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Sample records for cerdo sus scrofa

  1. Insectos asociados a fenómenos de descomposición cadavérica en cerdo blanco ( Sus scrofa en el municipio de Mosquera (Cundinamarca

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    Ospina Fonseca María Fernanda

    2002-11-01

    Full Text Available El estudio tuvo como objeto iniciar la investigación de los insectos asociados a fenómenos dedescomposición en una zona alta, específicamente en el municipio de Mosquera (Cundina-marca. Para lograrlo se usó como biomodelo cerdo blanco (Sus scrofa por su similitud conlos humanos, éste se sacrificó y se dejó al aire libre durante ocho meses. El proceso de des-composición se dividió en cinco fases: fresco (2 días, hinchado (13 días, descomposiciónactiva (15 días, descomposición avanzada (31 días y restos secos (160 días. Durante esteperiodo se recogieron 3.539 individuos, el 86.69% pertenecientes a Diptera y el 8.36% aColeoptera. Las familias más numerosas de Diptera fueron Muscidae (62.08% y Calliphoridae(14.44%, mientras que en Coleoptera fueron Silphidae (68.58% y Staphylinidae (14.86%.Se pudo observar la participación de los géneros Calliphora, Paralucilia y Chloroprocta, estosgéneros no aparecen en zonas bajas y podrían ser propios de zonas altas. No se presentarongrupos exclusivos para cada fase del proceso, aunque la presencia y abundancia de Diptera fuemayor al inicio de la descomposición, mientras que Coleoptera (exceptuando Silphidae fuemás abundante y activo al final del proceso. La lluvia ocasionó una "regresión" en la sucesiónde insectos. El presente trabajo no constituye una herramienta de carácter legal pero es elprimer trabajo sobre entomología forense realizado en la Sabana de Bogotá y es base paratrabajos posteriores en la zona.

  2. Caracterización etnozootécnica y potencial carnicero de Sus scrofacerdo criollo” en Latinoamérica.

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    Virginia Linares

    2011-01-01

    Full Text Available El objetivo de la presente revisión científica fue sistematizar información bibliográfica actualizada, concerniente a los aspectos genéticos, morfológicos, zootécnicos y potencial carnicero de los cerdos criollo s de Latinoamérica encontró una población de 73 millones de cerdos criollos en Latinoamérica, la mayoría de ellos criados bajo sistemas de producción extensivos, semiextensivos y agro pastoriles. Dado su origen europeo, la adaptación a los diferentes ec osistemas del continente y la introducción de razas ha devenido en una gran diversidad de cerdos criollos, lo que ha dado origen al aumento de variabilidad genética de los cerdos criollos latinos beneficiando su rusticidad, que involucra una mayor eficienc ia del sistema inmunológico. De la misma manera, su calidad de omnívoro le permitió una buena adaptación a regímenes alimentarios variados. Los cerdos criollos manifiestan un comportamiento reproductivo aceptable, tomando como referencia los indicadores de producción intensiva, precocidad sexual y alta viabilidad de los lechones al destete; sin embargo, su crecimiento es lento y su periodo de lactancia es largo. Con respecto a la calidad de sus cortes cárnicos, tomando en cuenta criterios subjetivos como la terneza y el valor nutricional, el cerdo criollo tiene mayor valor económico aventajando a los cerdos hipermagros.

  3. Bovine tuberculosis in a wild boar (Sus scrofa) in Poland.

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    Krajewska, Monika; Lipiec, Marek; Zabost, Anna; Augustynowicz-Kopeć, Ewa; Szulowski, Krzysztof

    2014-10-01

    Poland is officially tuberculosis free and bovine tuberculosis (BTB) cases are rarely found except in bovids. We found BTB in a wild boar (Sus scrofa) in the Bieszczady Mountains, southeastern Poland. Studies suggest possible transmission of infection between free-living European bison (Bison bonasus caucasicus) and wild boar in this area.

  4. The complete mitochondrial genome of European wild boar, Sus scrofa scrofa.

    Science.gov (United States)

    Hu, Xiao-Di; Yang, Xiao-Tian; Yang, En

    2016-09-01

    In this study, we report the complete mitochondrial genome sequence of the European wild boar, Sus scrofa scrofa for the first time. The genome is found to be 16,770 bp in length and has a base composition of A (34.63%), G (13.38%), C (26.21%), and T (25.78%), indicating that the percentage of A + T (60.41%) was higher than G + C (39.59%). Similar to other pigs, it contains a typically conserved structure including 13 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 1 control region (D-loop). Most of the genes were located on the H-strand except for the ND6 gene and eight tRNA genes. The complete mitochondrial genome sequence provided here would add a new genetic resource and new study on the evolution of the genus Sus.

  5. The Impact of Prophylactic Fasciotomy Following Porcine (Sus scrofa) Hind Limb Ischemia/Reperfusion Injury

    Science.gov (United States)

    2012-03-23

    ANSI Std. Z39.18 The Impact of Prophylactic Fasciotomy Following Porcine (Sus scrofa ) Hind Limb Ischemia/reperfusion Injury CAPT Thomas J...porcine model of hind limb ischemia. Method: Swine (Sus Scrofa ; 76 +/-6kg) were randomly assigned to no fasciotomy or prophylactic fasciotomy after...of ischemic intervals on neuromuscular recovery in a porcine (Sus scrofa ) survival model of extremity vascular injury. J Vasc Surg. 2011 Jan;53(1):165

  6. In Vitro Study of Caecal and Colon Microbial Fermentation Patterns in Wild Boar (Sus scrofa scrofa).

    Science.gov (United States)

    Pecka-Kiełb, Ewa; Bujok, Jolanta; Miśta, Dorota; Króliczewska, Bozena; Górecka, Justyna; Zawadzki, Wojciech

    2016-01-01

    The aim of this study was to evaluate wild boar (Sus scrofa scrofa) caecal and colon products of microbial activity including short chain fatty acids (SCFA), ammonia and methane concentrations. The in vitro method was applied to caecal and colon contents after 12 and 24-hour incubation with the substrate (wheat bran), or without any additive (control samples). The pH was also measured in each sample. In samples incubated with the substrate, a lower pH was noted as compared to the control (P < 0.001). In terms of the total SCFA concentration, the hindgut microbial fermentation pattern of wild boar was characterized by a high acetate level, followed by propionate and then butyrate at a ratio of 7:1.5:1. Substrate addition decreased acetate molar proportions (P < 0.001) and increased those of butyrate (P < 0.001) as well as propionate (P < 0.05). The total SCFA level in fresh, unincubated caecal samples (128 mmol/kg) was similar to that in the colon (111 mmol/kg). The ammonia concentrations were at the level of 0.8-1.5 mmol/kg of hindgut content and did not differ between the two investigated hindgut parts. Methanogenesis was also similar in the caecum and colon and after 24h was 2.69 mmol/kg and 2.27 for caecal colon control samples, respectively. The substrate increased total gas production and methane concentration (P < 0.001).

  7. Serological anthrax surveillance in wild boar (Sus scrofa) in Ukraine.

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    Bagamian, Karoun H; Skrypnyk, Artem; Rodina, Yana; Bezymennyi, Maksym; Nevolko, Oleg; Skrypnyk, Valeriy; Blackburn, Jason K

    2014-08-01

    Anthrax, caused by Bacillus anthracis, is an acute disease affecting wildlife, livestock, and humans worldwide, although its impact on these populations is underappreciated. In Ukraine, surveillance is passive, and anthrax is often detected in livestock. However, wildlife is not subject to surveillance, although anthrax deaths (such as in wild boar, Sus scrofa) have been documented. The wild boar is a plentiful and widespread species in Ukraine and is frequently hunted. We initiated a screening study testing Ukrainian wild boar blood samples for antibodies to B. anthracis. We mapped results relative to known livestock anthrax hotspots. We discovered evidence of exposure in wild boar up to 35 km from livestock anthrax hotspots and over 400 km from previous anthrax reports in boars. We make recommendations about using wildlife species as biosentinels for anthrax in Ukraine.

  8. Exome Capture with Heterologous Enrichment in Pig (Sus scrofa).

    Science.gov (United States)

    Guiatti, Denis; Pomari, Elena; Radovic, Slobodanka; Spadotto, Alessandro; Stefanon, Bruno

    2015-01-01

    The discovery of new protein-coding DNA variants related to carcass traits is very important for the Italian pig industry, which requires heavy pigs with higher thickness of subcutaneous fat for Protected Designation of Origin (PDO) productions. Exome capture techniques offer the opportunity to focus on the regions of DNA potentially related to the gene and protein expression. In this research a human commercial target enrichment kit was used to evaluate its performances for pig exome capture and for the identification of DNA variants suitable for comparative analysis. Two pools of 30 pigs each, crosses of Italian Duroc X Large White (DU) and Commercial hybrid X Large White (HY), were used and NGS libraries were prepared with the SureSelectXT Target Enrichment System for Illumina Paired-End Sequencing Library (Agilent). A total of 140.2 M and 162.5 M of raw reads were generated for DU and HY, respectively. Average coverage of all the exonic regions for Sus scrofa (ENSEMBL Sus_scrofa.Sscrofa10.2.73.gtf) was 89.33X for DU and 97.56X for HY; and 35% of aligned bases uniquely mapped to off-target regions. Comparison of sequencing data with the Sscrofa10.2 reference genome, after applying hard filtering criteria, revealed a total of 232,530 single nucleotide variants (SNVs) of which 20.6% mapped in exonic regions and 49.5% within intronic regions. The comparison of allele frequencies of 213 randomly selected SNVs from exome sequencing and the same SNVs analyzed with a Sequenom MassARRAY® system confirms that this "human-on-pig" approach offers new potentiality for the identification of DNA variants in protein-coding genes.

  9. Structural Classification of Wild Boar (Sus scrofa) Vocalizations.

    Science.gov (United States)

    Garcia, Maxime; Gingras, Bruno; Bowling, Daniel L; Herbst, Christian T; Boeckle, Markus; Locatelli, Yann; Fitch, W Tecumseh

    2016-04-01

    Determining whether a species' vocal communication system is graded or discrete requires definition of its vocal repertoire. In this context, research on domestic pig (Sus scrofa domesticus) vocalizations, for example, has led to significant advances in our understanding of communicative functions. Despite their close relation to domestic pigs, little is known about wild boar (Sus scrofa) vocalizations. The few existing studies, conducted in the 1970s, relied on visual inspections of spectrograms to quantify acoustic parameters and lacked statistical analysis. Here, we use objective signal processing techniques and advanced statistical approaches to classify 616 calls recorded from semi-free ranging animals. Based on four spectral and temporal acoustic parameters-quartile Q25, duration, spectral flux, and spectral flatness-extracted from a multivariate analysis, we refine and extend the conclusions drawn from previous work and present a statistically validated classification of the wild boar vocal repertoire into four call types: grunts, grunt-squeals, squeals, and trumpets. While the majority of calls could be sorted into these categories using objective criteria, we also found evidence supporting a graded interpretation of some wild boar vocalizations as acoustically continuous, with the extremes representing discrete call types. The use of objective criteria based on modern techniques and statistics in respect to acoustic continuity advances our understanding of vocal variation. Integrating our findings with recent studies on domestic pig vocal behavior and emotions, we emphasize the importance of grunt-squeals for acoustic approaches to animal welfare and underline the need of further research investigating the role of domestication on animal vocal communication.

  10. Differentiation of European wild boar (Sus scrofa scrofa) and domestic swine (Sus scrofa domestica) meats by PCR analysis targeting the mitochondrial D-loop and the nuclear melanocortin receptor 1 (MC1R) genes.

    Science.gov (United States)

    Fajardo, Violeta; González, Isabel; Martín, Irene; Rojas, Marı A; Hernández, Pablo E; Garcı A, Teresa; Martín, Rosario

    2008-03-01

    This work describes the differentiation of European wild boar (Sus scrofa scrofa) and domestic swine (Sus scrofa domestica) meats by PCR targeting sequences from two molecular markers: the mitochondrial displacement loop (D-loop) region and the nuclear melanocortin receptor 1 (MC1R) gene. A polymorphic D-loop fragment (∼270bp) was amplified and sequenced in a number of wild and domestic Sus scrofa meat samples, to find a nucleotide region suitable for PCR-RFLP analysis. Sequence data showed the presence of only a few point mutations across Sus scrofa D-loop sequences, not allowing direct discrimination between wild boar and domestic swine meats. Later, the MC1R gene was targeted and Sus scrofa-specific primers designed to amplify a 795bp MC1R fragment. Subsequent RFLP analysis of the MC1R swine-specific amplicons allowed selection of BspHI and BstUI endonucleases to carry out intraspecific Sus scrofa differentiation. Digestion of MC1R amplicons with the chosen enzymes generated characteristic PCR-RFLP profiles that allowed discrimination among meats from wild and domestic swine specimens. The technique also enabled the detection of samples that yielded heterozygous profiles, suggesting hybrids resulting from wild boar and domestic pig breeding. The PCR-RFLP reported here, targeting the MC1R gene may be routinely applied to verify the correct labelling of game products.

  11. Helmintos e protozoários em fezes de javalis (Sus scrofa scrofa criados em cativeiro Helminths and protozoa in wild boars (Sus scrofa scrofa feces raised in captivity

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    M.J.S. Mundim

    2004-12-01

    Full Text Available Faecal samples from 79 wild boars (Sus scrofa scrofa were examined by sedimentation method, zinc sulfate flotation method, and centrifugal flotation in sugar solution for endoparasites research. The results showed that 97.5% of the samples were positive for helminths and/or protozoa as follows: strongilides (70.9%, Ascaris suum (46.9%, Trichuris suis (29.1%, Metastrongylus sp. (12.6%, Strongyloides ransomi (3.8%, Balantidium coli (38.0%, Entamoeba spp. (15.2%, Giardia spp. (1.3%, Blastocystis sp. (12.6%. The frequency of Entamoeba was higher in young animals. Coccidian oocysts were observed in 59.5% samples and five species of Eimeria and one Isospora were recovered: Eimeria scabra (31.9%, E. deblieck (31.9%, E. perminuta (23.4%, E. cerdonis (17.0%, E. scrofae (12.8% and Isospora suis (12.8%.

  12. Development of a Physiologically Based Pharmacokinetic Model for the Anesthetics Halothane, Isoflurane, and Desflurane in the Pig (SUS SCROFA)

    Science.gov (United States)

    1999-08-01

    HALOTHANE, ISOFLURANE, AND DESFLURANE IN THE PIG ( SUS SCROFA ) / Allen Vinegar MANTECH-GEO CENTER JOINT VENTURE PO BOX 31009 ~ DAYTON, OH 45437-0009...Pharmacokinetic Model for the Anesthetics Contract F41624-96-C-9010 Halothane, Isoflurane, and Desfiurane in the Pig ( Sus Scrofa ) PE 62202F PR 7757 6. AUTHOR(S) TA...PFA) " CA Figure I - Physiologicallly Based Pharmacokinetic Model of the Pig ( Sus scrofa ). Abbreviations: CA, arterial concentration; CX, exhaled

  13. Does Glucagon Improve Survival in a Porcine (Sus Scrofa) of Adult Asphyxial Cardiac Arrest in Addition to Standard Epinephrine Therapy?

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    2012-01-17

    UDIIILI: oa. I..UN I ItA!.. I NUMDI:It Does Glucagon improve survival in a porcine (Sus Scrofa ) of adult asphyxial cardiac arrest in addition to...EXPIRATION DATE: 25 Mar 13 PROTOCOL TITLE: Does Glucagon Improve Survival in a Porcine (Sus scrofa ) Model of Adult Asphyxial Cardiac Arrest in Addition...Additions: Deletions: 2 Protocol No: A-2007-03 Protocol Title: Does Glucagon Improve Survival in a Porcine (Sus scrofa ) Model of Adult Asphyxial

  14. Sodium Thiosulfate Versus Hydroxocobalamin in the Treatment of Acute, Severe Cyanide Induced Cardiotoxicity in a Swine (Sus Scrofa) Model

    Science.gov (United States)

    2011-09-22

    IUIVIJ:lt:M Sodium thiosulfate versus hydroxocoba~amin in the treatment of acute, severe cyanide induced cardiotoxicity in a swine (Sus Scrofa )model on...treatment of acute, severe cyanide induced cardiotoxicity in a swine (Sus Scrofa ) model .. 4. Principal Investigator (PI): ’ ~ I ’ ·, .. Name Rank Date

  15. Mapping carcass and meat quality QTL on Sus Scrofa chromosome 2 in commercial finishing pigs

    NARCIS (Netherlands)

    Heuven, H.C.M.; van Wijk, R.H.J.; Dibbits, B.; van Kampen, T.A.; Knol, E.F.

    2009-01-01

    Genet Sel Evol. 2009 Jan 5;41:4. Mapping carcass and meat quality QTL on Sus Scrofa chromosome 2 in commercial finishing pigs. Heuven HC, van Wijk RH, Dibbits B, van Kampen TA, Knol EF, Bovenhuis H. Animal Breeding and Genomics Centre, Wageningen University, Wageningen, The Netherlands. h.c.m.heuven

  16. Absence of bovine tuberculosis in feral swine (Sus scrofa) from the Southern Texas border region

    Science.gov (United States)

    Free-ranging wildlife, like feral swine (Sus scrofa), harbor a variety of diseases that are infectious to livestock and could negatively impact agricultural production. Information is lacking regarding the exposure and infection rates for bovine tuberculosis (Mycobacterium bovis; bTB), and many othe...

  17. Comparison of methods of extracting messenger Ribonucleic Acid from ejaculated Porcine (Sus Scrofa) Spermatozoa

    Science.gov (United States)

    H. D. Guthrie, G.R. Welch, and L. A. Blomberg. Comparison of Methods of Extracting Messenger Ribonucleic Acid from Ejaculated Porcine (Sus Scrofa) Spermatozoa. Biotechnology and Germplasm Laboratory, Agricultural Research Service U. S. Department of Agriculture, Beltsville, MD 20705 The purpos...

  18. First isolation of Trichinella britovi from a wild boar (Sus scrofa) in Belgium.

    NARCIS (Netherlands)

    Schynts, F; Giessen, Joke van der; Tixhon, S; Pozio, E; Dorny, P; Borchgrave, J de

    2006-01-01

    Since 1992, when the European Union Council Directive requires that wild boars (Sus scrofa) hunted in EU for commercial purpose should be examined for Trichinella, the infection has not been detected in wild boars from Belgium, despite serological evidence of the presence of anti-Trichinella antibod

  19. Molekulargenetische und archäologische Untersuchungen zur Domestikation und Züchtung des Schweins (Sus scrofa)

    OpenAIRE

    Krause-Kyora, Ben

    2011-01-01

    Die Arbeit behandelt die Domestikation der Stammart des europäischen Wildschweins (Sus scrofa) und den Beginn und Umsetzung der Schweinehaltung im nordmitteleuropäischen Raum mit Hilfe von archäologischen und genetischen Analysemethoden.

  20. Detection of Echinococcus granulosus G3 in a Wild Boar ( Sus scrofa ) in Central Italy Using PCR and Sequencing.

    Science.gov (United States)

    Di Paolo, Antonella; Piseddu, Toni; Sebastianelli, Martina; Manuali, Elisabetta; Corneli, Sara; Paniccià, Marta; Papa, Paola; Viali, Selina; Mazzone, Piera

    2017-01-24

    We report cystic echinococcosis in a free-living wild boar ( Sus scrofa ) in Europe. Parasites were identified by histopathology and molecular techniques, revealing Echinococcus granulosus of the G3 genotype.

  1. On the evolutionary consequences of increasing litter size with multiple paternity in wild boar (Sus scrofa scrofa).

    Science.gov (United States)

    Gayet, Thibault; Devillard, Sébastien; Gamelon, Marlène; Brandt, Serge; Say, Ludovic; Baubet, Eric

    2016-06-01

    Understanding how some species may be able to evolve quickly enough to deal with anthropogenic pressure is of prime interest in evolutionary biology, conservation, and management. Wild boar (Sus scrofa scrofa) populations keep growing all over Europe despite increasing hunting pressure. In wild boar populations subject to male-selective harvesting, the initially described polygynous mating system may switch to a promiscuous/polyandrous one. Such a change in the mating system, where potentially more males sire a litter at one reproductive event, may be associated with the retention of high genetic diversity and an increase of litter size. We tested these hypotheses by estimating the number of sires per litter based on a six-year long monitoring of a wild boar population subject to particularly high harvesting pressure. Our results show a high and stable genetic diversity and high rates of multiple paternity compared to other populations, thus depicting a promiscuous/polyandrous mating system in this population. We also show that litter size is positively linked to the number of sires, suggesting that multiple paternity increases fecundity. We finally discuss that multiple paternity may be one of the factors allowing rapid evolution of this population by maintaining both genetic and phenotypic diversity.

  2. Development of molecular tools to differentiate Indian wild pig (Sus scrofa cristatus) meat from exotic and local domestic pig meat

    OpenAIRE

    Kajal Kumar Jadav; Avadh Bihari Shrivastav; Nidhi Rajput

    2013-01-01

    Aim: Identification of wild pig and domestic pig is essential to prevent illegal poaching of wild pig and to implement Wildlife (Protection) Act, 1972. PCR-RFLP was used to differentiate Wild pig (Sus scrofa cristatus) from Domestic pig (Sus scrofa domestica) meat. Materials and Methods: DNA was isolated from meat samples of both the sub species and a fragment of Cytochrome b gene was amplified using universal primers and the PCR products were subjected to restriction digestion. Results: All ...

  3. Genetic variability among the wild boars (Sus scrofa scrofa, crossbred animals and pigs using microsatellite markers (STRs

    Directory of Open Access Journals (Sweden)

    Paula Viana Correa da Silva

    2011-04-01

    Full Text Available The aim of this work was to study the genetic variability among the wild boars, crossbred animals and pigs using microsatellite markers. Five genetic groups were studied. The fragments of three microsatellites developed for Sus scrofa domestica - IGF1, ACTG2 and TNFB - were amplified through PCR technique to evaluate the expected intra populacion variability (He and observed (Ho heterozygosity, and endogamy coefficient (F IS within each population and inter population variability F IS , testing relationship among five genetic groups to establish the genetic distance among them. The high level of observed heterozygosity values varied between 0.537 and 0.7871. Generally, F IS was low, suggesting that the endogamy did not exist between the tested animals.

  4. Changes to soil bacterial profiles as a result of Sus scrofa domesticus decomposition.

    Science.gov (United States)

    Olakanye, Ayodeji O; Thompson, Tim; Komang Ralebitso-Senior, T

    2014-12-01

    The importance of cadaver decomposition knowledge for clandestine grave location cannot be over emphasised. Notwithstanding this, only a limited understanding is available on the resulting soil microbial community dynamics. To address this paucity, a pig leg (Sus scrofa domesticus; 5kg) was buried in freshly weighed (20kg) sandy loamy soil in a sealed microcosm (40cm height) in parallel with a soil only control. Both microcosms were perforated nine times at equal distances and maintained outside. Soil samples were collected through these perforations from the top (0-10cm), middle (10-20cm) and bottom (20-30cm) segments every three days for the first two weeks, and then weekly up to 14 weeks. PCR-DGGE gels quantified by 1D Phoretix showed increases in the cumulative soil community richness values of 43, 66 and 106 for the top, middle and bottom segments, respectively, in the presence of Sus scrofa domesticus. Shannon-Wiener's (H') and Simpon's (D) indices confirmed corresponding species diversity increases in the middle (H'=1.58-2.33; D=0.79-0.91) and bottom (H'=2.48-3.16; D=0.85-0.95) depths between days 10 and 71 compared with the control. In contrast, similar evenness was recorded for all segments in both the Sus scrofa domesticus and control soils.

  5. Cognitive testing of pigs (Sus scrofa) in translational biobehavioral research

    DEFF Research Database (Denmark)

    Kornum, Birgitte R; Knudsen, Gitte M

    2011-01-01

    Within neuroscience and biobehavioral research, the pig (Sus scrofus) is increasingly being acknowledged as a valuable large animal species. Compared to the rodent brain, the pig brain more closely resembles the human brain in terms of both anatomy and biochemistry, which associates the pig with ...

  6. Analysis of muscle and ovary transcriptome of Sus scrofa: assembly, annotation and marker discovery.

    Science.gov (United States)

    Nie, Qinghua; Fang, Meixia; Jia, Xinzheng; Zhang, Wei; Zhou, Xiaoning; He, Xiaomei; Zhang, Xiquan

    2011-10-01

    Pig (Sus scrofa) is an important organism for both agricultural and medical purpose. This study aims to investigate the S. scrofa transcriptome by the use of Roche 454 pyrosequencing. We obtained a total of 558 743 and 528 260 reads for the back-leg muscle and ovary tissue each. The overall 1 087 003 reads give rise to 421 767 341 bp total residues averaging 388 bp per read. The de novo assemblies yielded 11 057 contigs and 60 270 singletons for the back-leg muscle, 12 204 contigs and 70 192 singletons for the ovary and 18 938 contigs and 102 361 singletons for combined tissues. The overall GC content of S. scrofa transcriptome is 42.3% for assembled contigs. Alternative splicing was found within 4394 contigs, giving rise to 1267 isogroups or genes. A total of 56 589 transcripts are involved in molecular function (40 916), biological process (38 563), cellular component (35 787) by further gene ontology analyses. Comparison analyses showed that 336 and 553 genes had significant higher expression in the back-leg muscle and ovary each. In addition, we obtained a total of 24 214 single-nucleotide polymorphisms and 11 928 simple sequence repeats. These results contribute to the understanding of the genetic makeup of S. scrofa transcriptome and provide useful information for functional genomic research in future.

  7. Serologic tests for detecting antibodies against Mycobacterium bovis and Mycobacterium avium subspecies paratuberculosis in Eurasian wild boar (Sus scrofa scrofa).

    Science.gov (United States)

    Boadella, Mariana; Lyashchenko, Konstantin; Greenwald, Reena; Esfandiari, Javan; Jaroso, Raquel; Carta, Tania; Garrido, Joseba M; Vicente, Joaquín; de la Fuente, José; Gortázar, Christian

    2011-01-01

    New tools to detect exposure of free-range Eurasian wild boar (Sus scrofa scrofa) to pathogenic mycobacteria would be valuable for improved disease surveillance and wildlife management. Two hundred sera from wild boar of known Mycobacterium bovis infection status were used to evaluate test suitability for the detection of antibodies against M. bovis and Mycobacterium avium subsp. paratuberculosis (or cross-reacting members of the M. avium complex). Two traditional enzyme-linked immunosorbent assays were evaluated using M. bovis purified protein derivative (bPPD) and paratuberculosis protoplasmatic antigen 3 (PPA3) as antigens, respectively, and a new point-of-care test format for bovine tuberculosis (bTB) that uses the innovative dual-path platform (DPP TB) test. The effect of individual factors (sex, age, lesions) on the diagnostic performance of the serologic tests was also determined. Although the DPP had a sensitivity of 89.6% and a specificity of 90.4%, for bPPD, the sensitivity was 79.2% and the specificity 100%. Both tests had a kappa agreement of 0.80. Sixty-five of 68 (95.6%) wild boar sera with antibodies against the PPA3 antigen corresponded to known M. bovis-infected wild boar. Significant differences were not observed in the bPPD and DPP readings among lesion categories or between age classes. A slight sex-related difference in sensitivity toward males in the DPP was found, but it was not detected in the bPPD enzyme-linked immunosorbent assay. The results support the use of antibody-based diagnostic tests for both large-scale and individual bTB testing of Eurasian wild boar and suggest that wild boar cannot be used as sentinels for infections caused by M. avium complex members.

  8. Intravenous Cobinamide, a Novel Cyanide Antidote, versus Hydroxocobalamin in the Treatment of Acute Cyanide Toxicity and Apnea in a Swine (Sus scrofa) Model

    Science.gov (United States)

    2013-02-12

    treatment of acute, severe cyanide induced cardiotoxicity of severe hypotension and of cardiac arrest in a swine (Sus Scrofa ) model Intravenous...cobinamide, a novel cyanide antidote, versus hydroxocobalamin in the treatment of acute cyanide toxicity and apnea in a swine (Sus Scrofa ) model...hydroxocobalamin in the treatment of acute cyanide toxicity and apnea in a swine (Sus Scrofa ) model Background: Hydroxocobalamin (HOC) is an FDA approved

  9. Cognitive testing of pigs (Sus scrofa) in translational biobehavioral research

    DEFF Research Database (Denmark)

    Kornum, Birgitte R; Knudsen, Gitte M

    2011-01-01

    Within neuroscience and biobehavioral research, the pig (Sus scrofus) is increasingly being acknowledged as a valuable large animal species. Compared to the rodent brain, the pig brain more closely resembles the human brain in terms of both anatomy and biochemistry, which associates the pig...... with a higher translational value. Several brain disorders have been fully or partially modeled in the pig and this has further spurred an interest in having access to behavioral tasks for pigs, and in particular to cognitive tasks. Cognitive testing of pigs has been conducted for several years by a small group...... of farm animal welfare researchers, but it has only recently received interest in the wider neuroscience community. Several behavioral tasks have successfully been adapted to the pig, and valuable results have been produced. However, most tasks have only been established at a single research facility...

  10. Cloning and Sequence Analysis of Sus scrofa interferon epsilon-1 Gene%Sus scrofa interferon epsilon-1基因的克隆与序列分析

    Institute of Scientific and Technical Information of China (English)

    杜会坡; 台玉磊; 王伟杰; 杨国宇

    2012-01-01

    [目的]克隆分析Sus scrofa interferon epsilon-I基因,以期为其生物学功能研究奠定基础.[方法]利用Homo sapiens interferon eμsilon l(IFNEI)序列(NM_176891.3)对猪HTG库进行搜索,通过对获得的2个片断(CU074336、AC 127471)的序列分析,在5’- UTR和3’-UTR设计I对克隆引物,对7日龄仔猪的胃组织进行RT - PCR,将PCR产物克隆、测序,并进行相关分析.[结果]同源性分析结果表明,猪SIFNEI与人、小鼠interferon epsilon-1基因cDNA编码区(CDS)的同源性分别为83.6%和69 2%;蛋白序列同源性分别为76.2%和55.2%.推测其氨基酸序列信号肽为第1~21位氨基酸,IFabd结构域为第59~176位氨基酸,结构特征与人、小鼠的interferon epsilon-1相一致.[结论]该研究克隆了Sus scrofa interferon epsilon-1基因,为进一步研究SIFNE1基因的生物学功能奠定了基础。%[ Objective] To clone and analyze the Sus scrofa interfemn epsiton-I gene, so as to lay foundation for the study of its biological func-tions. [ Method] The swine HTG database was searched with the Homo sapiens interferon epsilon-I (1FNEI) sequence (NM_176891.3) , two fragments (CU074336 and AC127471) were obtained and analyzed by sequencing. One pair of primers was cloned at 5'-UTR and 3'-UTR to analyze the gastric tissue of seven-day-old piglets by RT-PCR, the PCR products were cloned, sequenced and correlation analyzed. [ Result] The homology analysts showed that the homology of swine SIFNEI with human and mice interferon epsilon-I gene cDNA CDS was 83.6% and 69.2% respectively, the homology between their protein sequences was 76. 2% and 55.2% respectively. The signal peptide of ami no acid se-quence was predicted to be the 1 - 12 position of amino acid, the protein domains of IFabd were the 59 - 176 position of amino acid, which was consistent with the interferon epsilon-I of human and mice. [Conclusion] The study cloned the Sus scrofa interferon epsilon-I gene, and laid foundation for

  11. Occurrence and first molecular characterization of Sarcocystis spp. in wild boars (Sus scrofa) and domestic pigs (Sus scrofa domesticus) in Romania: Public health significance of the isolates.

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    Imre, Kálmán; Sala, Claudia; Morar, Adriana; Imre, Mirela; Ciontu, Cătălin; Chisăliță, Ion; Dudu, Andreea; Matei, Marius; Dărăbuș, Gheorghe

    2017-03-01

    Domestic and wild pigs, as intermediate hosts, can harbor tissue cysts of three Sarcocystis species namely S. miescheriana, S. suihominis and S. porcifelis. Out of them, S. suihominis is zoonotic. Romania is a country with high consumption of raw and/or undercooked traditional pork products. This fact may greatly favor the acquiring of the zoonotic Sarcocystis infections by humans, as definitive host. Based on this consideration and in order to investigate the occurrence and public health significance of Sarcocystis spp. in two western counties (Caraş-Severin and Timiş) of Romania, a total of 165 heart samples from hunted wild boars (Sus scrofa, n=101) and home slaughtered domestic pigs (Sus scrofa domesticus, n=64) were screened using microscopic fresh examination and molecular methods. Microscopic examination revealed the presence of sarcocysts in 60.4% of wild boars, and 23.4% of domestic pigs. Genetic characterization of isolates through the PCR-RFLP procedure, targeting the 18S rRNA gene, was successfully achieved for all microscopically positive samples, indicating the presence of a single species, S. miescheriana, in both hosts. The identity of 13 selected S. miescheriana isolates was also confirmed through sequencing. The tested hosts older than 27 months were found to be significantly higher infected (p<0.05) with Sarcocystis than the 6 to ≤27months age group. Although the human infective S. suihominis has not been registered, for a more reliable epidemiological picture, further molecular studies enrolling a larger number of animals and diagnosis on human intestinal Sarcocystis infections are still necessary.

  12. Cognitive testing of pigs (Sus scrofa) in translational biobehavioral research.

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    Kornum, Birgitte R; Knudsen, Gitte M

    2011-01-01

    Within neuroscience and biobehavioral research, the pig (Sus scrofus) is increasingly being acknowledged as a valuable large animal species. Compared to the rodent brain, the pig brain more closely resembles the human brain in terms of both anatomy and biochemistry, which associates the pig with a higher translational value. Several brain disorders have been fully or partially modeled in the pig and this has further spurred an interest in having access to behavioral tasks for pigs, and in particular to cognitive tasks. Cognitive testing of pigs has been conducted for several years by a small group of farm animal welfare researchers, but it has only recently received interest in the wider neuroscience community. Several behavioral tasks have successfully been adapted to the pig, and valuable results have been produced. However, most tasks have only been established at a single research facility, and would benefit from further validation. This review presents the cognitive tasks that have been developed for pigs, their validation, and their current use.

  13. A Serosurvey for Brucellosis in Wild Boar (Sus scrofa) in Sardinia, Italy.

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    Pilo, Cristian; Addis, Giuseppe; Deidda, Manuela; Tedde, Maria Tania; Liciardi, Manuele

    2015-10-01

    Porcine brucellosis is a zoonotic disease caused by Brucella suis and hosted by pigs (Sus scrofa). Both domestic pigs and wild boars are affected. We measured the prevalence of antibody to Brucella spp. in wild boars in Sardinia, Italy. During 1 November 2009 to 31 January 2010, we collected 570 serum samples from legally hunted wild boars and tested them using a commercial competitive enzyme-linked immunosorbent assay. Sex and age class of the sampled wild boars were also recorded. Thirty-five samples were positive for an apparent antibody prevalence of 6.1%. Antibody prevalences did not differ between sexes or among age classes.

  14. Prevalence of antibodies to Toxoplasma gondii in South Korean wild boar (Sus scrofa coreanus).

    Science.gov (United States)

    Jeong, Wooseog; Yoon, Hachung; Kim, Yong Kwan; Moon, Oun-Kyong; Kim, Do-Soon; An, Dong-Jun

    2014-10-01

    Abstract Toxoplasma gondii is an obligate intracellular protozoan parasite and a commonly encountered pathogen in humans and animals. The wild boar (Sus scrofa coreanus) is considered a good indicator when monitoring environmental contamination by T. gondii. We surveyed the prevalence of antibodies against T. gondii in wild boars from South Korea. Blood samples were collected from 426 wild boars captured in eight provinces of South Korea during the hunting seasons in 2008-12. Antibodies against T. gondii were detected using an indirect enzyme-linked immunosorbent assay in samples from 152 of boars, indicating an overall antibody prevalence of 36% (95% confidence interval=31-40%).

  15. Cross-Reactivity of Porcine Immunoglobulin A Antibodies with Fecal Immunoglobulins of Wild Boar (Sus scrofa) and Other Animal Species.

    Science.gov (United States)

    Seo, Sang Won; Yoo, Sung J; Sunwoo, Sunyoung; Hyun, Bang Hun; Lyoo, Young S

    2016-06-01

    Fecal samples obtained from wild boar habitats are useful for the surveillance of diseases in wild boar populations; however, it is difficult to determine the species of origin of feces collected in natural habitats. In this study, a fecal IgA ELISA was evaluated as a method for identifying the porcine species from fecal samples. Both domestic pigs (Sus scrofa domestica) and wild boars (Sus scrofa coreanus) showed significantly higher levels of fecal IgA than other animal species. Additionally, age dependent changes in the level of Ig A in wild boars and domestic pigs were identified; Titers of Ig A were highest in suckling period and lowest in weanling period.

  16. Comparison of two codon optimization strategies enhancing recombinant Sus scrofa lysozyme production in Pichia pastoris.

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    Zhu, D; Cai, G; Wu, D; Lu, J

    2015-05-16

    Lysozyme has played an important role in animal feed additive industry, food additive industry and biological engineering. For improving expression efficiency of recombinant lysozyme from Sus scrofa, two genes respectively designed by the most used codon optimization strategies, "one amino acid one codon" and "codon randomization", were synthesized and expressed in Pichia pastoris X—33. At shaking flask level, Sus scrofa lysozyme (SSL) under two conditions had a highest activity of 153.33±10.41 and 538.33±15.18 U/mL after a 5 days induction of 1% methanol, with secreted protein concentration 80.03±1.94 and 239.60±4.16 mg/L, respectively. Compared with the original SSL gene, the expression of optimized SSL gene by the second strategy showed a 2.6 fold higher level, while the first method had no obvious improvement in production. In total secreted protein, the proportions of recombinant SSL encoded by the original gene, first method optimized gene and the second—strategy optimized one were 75.06±0.25%, 74.56±0.14% and 79.00±0.14%, respectively, with the same molecular weight about 18 kDa, optimum acidity pH 6.0 and optimum temperature 35degC.

  17. Cryptosporidium suis and Cryptosporidium scrofarum in Eurasian wild boars (Sus scrofa) in Central Europe.

    Science.gov (United States)

    Němejc, Karel; Sak, Bohumil; Květoňová, Dana; Hanzal, Vladimír; Janiszewski, Paweł; Forejtek, Pavel; Rajský, Dušan; Ravaszová, Petra; McEvoy, John; Kváč, Martin

    2013-11-08

    From 2011 to 2012, to identify Cryptosporidium spp. occurrence in Eurasian wild boars (Sus scrofa) 29 randomly selected localities (both forest areas and enclosures) across the Central European countries of Austria, the Czech Republic, Poland, and the Slovak Republic were investigated. Cryptosporidium oocysts were microscopicaly detected in 11 out of 460 faecal samples examined using aniline-carbol-methyl violet staining. Sixty-one Cryptosporidium infections, including the 11 infections that were detected by microscopy, were detected using genus- or species-specific nested PCR amplification of SSU rDNA. This represents a 5.5 fold greater sensitivity for PCR relative to microscopy. Combining genus- and species-specific PCR tools significantly changes the perspective on the occurrence of Cryptosporidium spp. in wild boars. While RFLP and direct sequencing of genus specific PCR-amplified products revealed 56 C. suis (20) and C. scrofarum (36) monoinfections and only 5 mixed infections of these species, species-specific molecular tools showed 44 monoinfections and 17 mixed infections with these species. PCR analysis of the gp60 gene did not reveal any other Cryptosporidium infections. Similar to domestic pigs, C. scrofarum was detected as a dominant species infecting adult Eurasian wild boars (Sus scrofa). Cryptosporidium infected wild boars did not show signs of clinical disease. This report is perhaps the most comprehensive survey of cryptosporidial infection in wild boars.

  18. Genetic characterization of the domestic pig (Sus scrofa domestica in Cerete-Colombia, using microsatellite markers

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    Iván Meléndez G.

    2014-06-01

    Full Text Available Objective. The purpose of this study was to characterize a population of domestic pig (Sus scrofa domestica in Cereté, Córdoba, using 20 microsatellite; calculate heterozygosity per locus and average heterozygosity. Materials and methods. Hair samples were collected from 62 specimens. DNA was extracted by proteinase K digestion and phenol-chloroform purification. Information from 20 microsatellites was selected out of those recommended for swine biodiversity studies. PCR products were separated by a vertical polyacrylamide gel electrophoresis. The bands were visualized by staining with silver nitrate. Results. All microsatellites used were polymorphic. Between 3 (SW1067 and 15 (IFNG alleles were detected with an average number of 6.7 and a total de 134 alleles. The average expected and observed heterozygosities were 0.5278 and 0.5479, respectively. PIC values ranged between 0.1999 and 0.8300 for loci SW1067 and SW911, respectively. Conclusions. Levels of observed and expected heterozygosity found in the present study indicate that the domestic pig (Sus scrofa domestica in Córdoba Cereté show high degree of genetic variability

  19. Cytochrome b based genetic differentiation of Indian wild pig (Sus scrofa cristatus) and domestic pig (Sus scrofa domestica) and its use in wildlife forensics.

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    Gupta, Sandeep Kumar; Kumar, Ajit; Hussain, Syed Ainul; Vipin; Singh, Lalji

    2013-06-01

    The Indian wild pig (Sus scrofa cristatus) is a protected species and listed in the Indian Wildlife (Protection) Act, 1972. The wild pig is often hunted illegally and sold in market as meat warranting punishment under law. To avoid confusion in identification of these two subspecies during wildlife forensic examinations, we describe genetic differentiation of Indian wild and domestic pigs using a molecular technique. Analysis of sequence generated from the partial fragment (421bp) of mitochondrial DNA (mtDNA) cytochrome b (Cyt b) gene exhibited unambiguous (>3%) genetic variation between Indian wild and domestic pigs. We observed nine forensically informative nucleotide sequence (FINS) variations between Indian wild and domestic pigs. The overall genetic variation described in this study is helpful in forensic identification of the biological samples of wild and domestic pigs. It also helped in differentiating the Indian wild pig from other wild pig races. This study indicates that domestic pigs in India are not descendent of the Indian wild pig, however; they are closer to the other wild pig races found in Asia and Europe.

  20. Exploring PTX3 expression in Sus scrofa cardiac tissue using RNA sequencing.

    Science.gov (United States)

    Cabiati, Manuela; Caselli, Chiara; Savelli, Sara; Prescimone, Tommaso; Lionetti, Vincenzo; Giannessi, Daniela; Del Ry, Silvia

    2012-02-10

    The prototypic long pentraxin PTX3 is a novel vascular inflammatory marker sharing similarities with the classic short pentraxin (C-reactive protein). PTX3 is rapidly produced and released by several cell types in response to local inflammation of the cardiovascular system. Plasma PTX3 levels are very low in normal conditions and increase in heart failure (HF) patients with advancing NYHA functional class, but its exact role during HF pathogenetic mechanisms is not yet established. No data about PTX3 cardiac expression in normal and pathological conditions are currently available, either in human or in large-size animals. Of the latter, the pig has a central role in "in vivo" clinical settings but its genome has not been completely sequenced and the PTX3 gene sequence is still lacking. The aim of this study was to sequence the PTX3 in Sus scrofa, whose sequence is not yet present in GenBank. Utilizing our knowledge of this sequence, PTX3 mRNA expression was evaluated in cardiac tissue of normal (n=6) and HF pigs (n=5), obtained from the four chambers. To sequence PTX3 gene in S. scrofa, the high homology between Homo sapiens and S. scrofa was exploited. Pig PTX3 mRNA was sequenced using polymerase chain reaction primers designed from human consensus sequences. The DNA, obtained from different RT-PCR reactions, was sequenced using the Sanger method. S. scrofa PTX3 mRNA, 1-336 bp, was submitted to GenBank (ID: GQ412351). The sequence obtained from pig cardiac tissue shared an 84% sequence identity with human homolog. The presence of PTX3 mRNA expression was detected in all the cardiac chambers sharing an increase after 3 weeks of pacing compared to controls (p=0.036 HF right atrium vs. N; p=0.022, HF left ventricle vs. N). Knowledge of the PTX3 sequence could be a useful starting point for future studies devoted to better understanding the specific role of this molecule in the pathogenesis of cardiovascular diseases.

  1. High prevalence of hepatitis E virus in wild boar (Sus scrofa) in Yamaguchi Prefecture, Japan.

    Science.gov (United States)

    Hara, Yuka; Terada, Yutaka; Yonemitsu, Kenzo; Shimoda, Hiroshi; Noguchi, Keita; Suzuki, Kazuo; Maeda, Ken

    2014-04-01

    Hepatitis E virus (HEV) causes a food- and water-borne disease in humans, and Japanese wild boar (Sus scrofa leucomystax) meat is one of the most important sources of infection in Japan. We tested 113 serum samples from wild boar captured in Shimonoseki City, Yamaguchi Prefecture, Japan from 2010 to 2012. Serum samples were tested by enzyme-linked immunosorbent assay (ELISA) using virus-like particles as antigen and nested reverse-transcription PCR (RT-PCR). Anti-HEV IgG antibodies were detected in 47 of the 113 wild boar serum samples (42%), and HEV RNA was detected in five samples (4%). Sequence analysis showed that the five HEV isolates belonged to genotype 4, forming a cluster with a previous isolate from a human hepatitis E case in this region in 2011. These results indicate that wild boar in this region are infected with potentially pathogenic HEV at a high prevalence.

  2. Congenital toxoplasmosis in wild boar (Sus scrofa) and identification of the Toxoplasma gondii types involved.

    Science.gov (United States)

    Calero-Bernal, Rafael; Gómez-Gordo, Luis; Saugar, José María; Frontera, Eva; Pérez-Martín, Juan Enrique; Reina, David; Serrano, Francisco Javier; Fuentes, Isabel

    2013-10-01

    Congenital toxoplasmosis has been little described in wild animals. We report a case of vertical transmission in wild boar (Sus scrofa). Necropsy and histopathologic examination of a pregnant female and her three fetuses revealed all to have lesions compatible with acute toxoplasmosis. Nested polymerase chain reaction B1 gene detected Toxoplasma gondii in maternal (heart and diaphragm) and fetal (central nervous system, retina, optic nerve, heart, lung, tongue, and diaphragm) samples. The mother had a mixed infection of T. gondii types I and III. One fetus with type III infection developed no malformations, but the others-one with type I infection and one infected by types I and III-showed bilateral ocular agenesis, prognathism, and agenesis of the nasal cartilage. These results suggest the pathogenicity of the various T. gondii types may differ in wild boars.

  3. Microsatellite markers for identification and parentage analysis in the European wild boar (Sus scrofa

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    Costa Vânia

    2012-09-01

    Full Text Available Abstract Background The wild boar (Sus scrofa is among the most widespread mammal species throughout the old world. Presently, studies concerning microsatellites in domestic pigs and wild boars have been carried out in order to investigate domestication, social behavior and general diversity patterns among either populations or breeds. The purpose of the current study is to develop a robust set of microsatellites markers for parentage analyses and individual identification. Findings A set of 14 previously reported microsatellites markers have been optimized and tested in three populations from Hungary, Portugal and Spain, in a total of 167 samples. The results indicate high probabilities of exclusion (0.99999, low probability of identity (2.0E-13 – 2.5E-9 and a parentage assignment of 100%. Conclusions Our results demonstrate that this set of markers is a useful and efficient tool for the individual identification and parentage assignment in wild boars.

  4. The Effect of Clothing on the Rate of Decomposition and Diptera Colonization on Sus scrofa Carcasses.

    Science.gov (United States)

    Card, Allison; Cross, Peter; Moffatt, Colin; Simmons, Tal

    2015-07-01

    Twenty Sus scrofa carcasses were used to study the effect the presence of clothing had on decomposition rate and colonization locations of Diptera species; 10 unclothed control carcasses were compared to 10 clothed experimental carcasses over 58 days. Data collection occurred at regular accumulated degree day intervals; the level of decomposition as Total Body Score (TBSsurf ), pattern of decomposition, and Diptera present was documented. Results indicated a statistically significant difference in the rate of decomposition, (t427  = 2.59, p = 0.010), with unclothed carcasses decomposing faster than clothed carcasses. However, the overall decomposition rates from each carcass group are too similar to separate when applying a 95% CI, which means that, although statistically significant, from a practical forensic point of view they are not sufficiently dissimilar as to warrant the application of different formulae to estimate the postmortem interval. Further results demonstrated clothing provided blow flies with additional colonization locations.

  5. Seroprevalence of Neospora caninum in feral swine (Sus scrofa) in the United States.

    Science.gov (United States)

    Cerqueira-Cézar, Camila K; Pedersen, Kerri; Calero-Bernal, Rafael; Kwok, Oliver C; Villena, Isabelle; Dubey, Jitender P

    2016-08-15

    The protozoon Neospora caninum is a major cause of abortion in cattle worldwide. Canids (Canis familiaris, Canis latrans, Canis lupus) are definitive hosts whereas many other animal species, including pigs, are intermediate hosts for the parasite. Between 2012 and 2014, serum samples from 1059 feral swine (Sus scrofa) from 29 states of the USA were tested for N. caninum antibodies, using the N. caninum agglutination test (NAT). Of these, 159 (15.0%) feral pigs from 21 states tested positive, with a range of titers of 1:25 (cut-off) (n=153), 1:200 (1), 1:400 (1), 1:800 (3) and 1:3200 (1). Results indicate widespread exposure of feral swine to N. caninum infection across the USA.

  6. Caries, Periodontal Disease, Supernumerary Teeth and Other Dental Disorders in Swedish Wild Boar (Sus scrofa).

    Science.gov (United States)

    Malmsten, A; Dalin, A-M; Pettersson, A

    2015-07-01

    Between January and December 2013, the dental and periodontal health of 99 Swedish wild boars (Sus scrofa) was investigated. Sampling occurred in conjunction with routine hunting at six large estates in the southern and middle parts of Sweden. All six of the estates use supplemental feeding. The weight of the animals, their sex and their dates of death were noted. Age was estimated using tooth eruption and tooth replacement patterns. The oral cavity was inspected and abnormalities were recorded on a dental chart modified for wild boars. The findings included supernumerary teeth, absence of teeth, mild class II malocclusion, severe tooth wear, periodontitis, calculus, caries, tooth fractures and the presence of enamel defects. Swedish wild boars suffer from different dental lesions and the impact of supplemental feeding on dental and periodontal health is still to be investigated.

  7. Shifts in soil biodiversity-A forensic comparison between Sus scrofa domesticus and vegetation decomposition.

    Science.gov (United States)

    Olakanye, Ayodeji O; Thompson, Tim; Ralebitso-Senior, T Komang

    2015-12-01

    In a forensic context, microbial-mediated cadaver decomposition and nutrient recycling cannot be overlooked. As a result, forensic ecogenomics research has intensified to gain a better understanding of cadaver/soil ecology interactions as a powerful potential tool for forensic practitioners. For this study, domestic pig (Sus scrofa domesticus) (4g) and grass (Agrostis/Festuca spp) cuttings (4g) were buried (July 2013 to July 2014) in sandy clay loam (80 g) triplicates in sealed microcosms (127 ml; 50 × 70 cm) with parallel soil only controls. The effects of the two carbon sources were determined by monitoring key environmental factors and changes in soil bacterial (16S rRNA gene) and fungal (18S rRNA gene) biodiversity. Soil pH changes showed statistically significant differences (pscrofa domesticus and grass trimming decomposition, respectively. In contrast, no statistically significant difference in evenness (p>0.05) was observed between the treatments.

  8. FATAL CASE OF STREPTOCOCCUS SUIS INFECTION IN A YOUNG WILD BOAR (SUS SCROFA) FROM SOUTHWESTERN SPAIN.

    Science.gov (United States)

    Risco, David; Fernández-Llario, Pedro; Cuesta, Jesús M; García-Jiménez, Waldo L; Gonçalves, Pilar; Martínez, Remigio; García, Alfredo; Rosales, Rubén; Gómez, Luis; de Mendoza, Javier Hermoso

    2015-06-01

    Streptococcus suis is a recognized pathogen that may cause important diseases in pigs and humans. This microorganism has been repeatedly isolated from wild boar (Sus scrofa). However, its health implications for this wild species are still unknown. This article reports a detailed description of a fatal case of septicemia by S. suis affecting a young wild boar. The affected animal, about 15 days old, was found near death and exhibiting neurologic signs at a wild boar estate in southwestern Spain. Postmortem examination showed generalized congestion, brain hemorrhages and lobular pneumonia. Histopathological evaluation demonstrated the presence of meningitis and encephalitis with marked congestion and suppurative bronchopneumonia. Streptococcus suis serotype 2 isolates exhibiting important virulence factors (extracellular factor, muramidase-released protein, and suylisin) were isolated from the affected animal. This study confirms the presence of potentially virulent and zoonotic strains of S. suis in wild boar from Spain.

  9. Lead and cadmium in wild boar (Sus scrofa) in the Sierra Nevada Natural Space (southern Spain).

    Science.gov (United States)

    Mulero, Rocío; Cano-Manuel, Javier; Ráez-Bravo, Arián; Pérez, Jesús M; Espinosa, José; Soriguer, Ramón; Fandos, Paulino; Granados, José E; Romero, Diego

    2016-08-01

    The aims of the present study were to investigate Pb and Cd levels in tissues of wild boar (Sus scrofa) from the Sierra Nevada Natural Space (SNNS) (southern Spain). Heavy metal concentrations in livers, kidneys and bones from 111 animals were determined by inductively coupled plasma optical emission spectrometry (ICP-OES). Bones and kidneys were the most Pb- and Cd-contaminated tissues, respectively; Cd concentrations were 5.6 times higher in kidneys than in livers. This is the first biomonitoring study of these pollutants in wild boar tissues in the SNNS, and findings indicate that this population is chronically exposed to these heavy metals. The detected Pb and Cd concentrations were lower than those found in many studies performed in Europe on the same species.

  10. Parasites of the respiratory tract of Sus scrofa scrofa (wild boar) from commercial breeder in southern Brazil and its relationship with Ascaris suum.

    Science.gov (United States)

    da Silva, Diego; Müller, Gertrud

    2013-03-01

    This study aimed to identify the species of helminths infecting the respiratory tract of Sus scrofa scrofa from commercial breeding and check the existence of a possible antagonistic relationship of these species with Ascaris suum. Forty wild boars were analyzed, and the genus Metastrongylus was recorded in the bronchi and bronchioles of 60 % of these, with the occurrence of the species Metastrongylus apri, Metastrongylus salmi, and Metastrongylus pudendotectus. The highest prevalence found was in M. apri (52.5 %), followed by M. salmi (20 %), and M. pudendotectus (7.5 %), registering the highest prevalence of Metastrongylus in wild boars from commercial breeding so far. M. apri was first reported parasitizing wild boars bred in captivity. There was no observed significant influence of A. suum in the mean intensity of Metastrongylus.

  11. Anatomia dos ramos linguais do nervo hipoglosso em Sus scrofa domesticus, L., 1758 = Anatomy of the lingual branches of the hypoglossal nerve in Sus scrofa domesticus, L., 1758

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    Patrícia Marques Fortes

    2009-01-01

    Full Text Available Estudamos as ramificações e distribuição do nervo hipoglosso após estepenetrar na raiz da língua (36 antímeros de suínos (Sus scrofa domesticus, L. 1758 adultos de ambos os sexos abatidos em frigorífico (região metropolitana de Goiânia, Estado de Goiás -Brasil. O material foi resfriado para transporte (4ºC, fixado em solução aquosa de formaldeído (7% por 72h, imerso em solução aquosa de ácido nítrico (15% por 72h, dissecado sob lupa (RASOR, II-20. Nos antímeros, observou-se o tronco e ramos nervosos primários, secundários e terciários (método de BITTENCOURT et al., 1987. Seguimos a topográfica do nervo, a partir da região retro mandibular, entre as estruturas miofaciais do milohioideo e hipoglosso. Na raiz da língua, o nervo cruza a artéria lingual, passando a sermedial em relação a esta. Subdivide-se em três ordens de grandeza em direção ao músculo longitudinal superior, como segue: antímero esquerdo, quatro a 13 ramos primários, quatro a 21 ramos secundários e zero a 16 ramos terciários; no antímero direito, oito a 18 ramosprimários, três a 13 ramos secundários e zero a 12 ramos terciários. Em 27,59% dos antímeros analisados, os ramos do nervo hipoglosso estabeleceram junções com fibras do nervo lingual do antímero correspondente.We studied the ramifications and distribution of the hypoglossalnerve after it penetrated the root of the tongue (36 antimeres of adult swine (Sus scrofa domesticus, L. 1758 from both sexes slaughtered in a slaughterhouse (metropolitan region of Goiânia, Goiás State - Brazil. The material was chilled for transportation (4°C; placed in aqueous solution of formaldehyde (7% for 72 hours; submerged in aqueous solution of nitric acid (15% for 72 hours; dissected under magnifying glass (RASOR, II-20. The torso and the primary, secondary and tertiary nervous branches were observed in the antimeres (method of BITTENCOURT et al., 1987. We followed the topography of the nerve

  12. Characterization of Sus scrofa small non-coding RNAs present in both female and male gonads.

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    Dorota Kowalczykiewicz

    Full Text Available Small non-coding RNAs (sncRNAs are indispensable for proper germ cell development, emphasizing the need for greater elucidation of the mechanisms of germline development and regulation of this process by sncRNAs. We used deep sequencing to characterize three families of small non-coding RNAs (piRNAs, miRNAs, and tRFs present in Sus scrofa gonads and focused on the small RNA fraction present in both male and female gonads. Although similar numbers of reads were obtained from both types of gonads, the number of unique RNA sequences in the ovaries was several times lower. Of the sequences detected in the testes, 2.6% of piRNAs, 9% of miRNAs, and 10% of tRFs were also present in the ovaries. Notably, the majority of the shared piRNAs mapped to ribosomal RNAs and were derived from clustered loci. In addition, the most abundant miRNAs present in the ovaries and testes are conserved and are involved in many biological processes such as the regulation of homeobox genes, the control of cell proliferation, and carcinogenesis. Unexpectedly, we detected a novel sncRNA type, the tRFs, which are 30-36-nt RNA fragments derived from tRNA molecules, in gonads. Analysis of S. scrofa piRNAs show that testes specific piRNAs are biased for 5' uracil but both testes and ovaries specific piRNAs are not biased for adenine at the 10th nucleotide position. These observations indicate that adult porcine piRNAs are predominantly produced by a primary processing pathway or other mechanisms and secondary piRNAs generated by ping-pong mechanism are absent.

  13. Characterization of Sus scrofa small non-coding RNAs present in both female and male gonads.

    Science.gov (United States)

    Kowalczykiewicz, Dorota; Świercz, Aleksandra; Handschuh, Luiza; Leśniak, Katarzyna; Figlerowicz, Marek; Wrzesinski, Jan

    2014-01-01

    Small non-coding RNAs (sncRNAs) are indispensable for proper germ cell development, emphasizing the need for greater elucidation of the mechanisms of germline development and regulation of this process by sncRNAs. We used deep sequencing to characterize three families of small non-coding RNAs (piRNAs, miRNAs, and tRFs) present in Sus scrofa gonads and focused on the small RNA fraction present in both male and female gonads. Although similar numbers of reads were obtained from both types of gonads, the number of unique RNA sequences in the ovaries was several times lower. Of the sequences detected in the testes, 2.6% of piRNAs, 9% of miRNAs, and 10% of tRFs were also present in the ovaries. Notably, the majority of the shared piRNAs mapped to ribosomal RNAs and were derived from clustered loci. In addition, the most abundant miRNAs present in the ovaries and testes are conserved and are involved in many biological processes such as the regulation of homeobox genes, the control of cell proliferation, and carcinogenesis. Unexpectedly, we detected a novel sncRNA type, the tRFs, which are 30-36-nt RNA fragments derived from tRNA molecules, in gonads. Analysis of S. scrofa piRNAs show that testes specific piRNAs are biased for 5' uracil but both testes and ovaries specific piRNAs are not biased for adenine at the 10th nucleotide position. These observations indicate that adult porcine piRNAs are predominantly produced by a primary processing pathway or other mechanisms and secondary piRNAs generated by ping-pong mechanism are absent.

  14. Differential anti-influenza activity among allelic variants at the Sus scrofa Mx1 locus.

    Science.gov (United States)

    Palm, M; Leroy, M; Thomas, A; Linden, A; Desmecht, D

    2007-02-01

    A promising way to oppose infectious challenges would be to improve the resistance of the target species through genetic selection. Theoretically, a candidate gene is available against influenza viruses since a resistance trait was fortuitously discovered in the A2G mouse strain. This trait was demonstrated to be correlated with the expression of a specific isoform of the type I interferon (IFN)-dependent protein MX, an isoform coded by a specific allele at the mouse Mx1 locus. Two allelic polymorphisms were described recently in the Sus scrofa homologous gene. In this study, the frequencies and distribution of both alleles were evaluated among European domestic pig and wild boar populations by PCR-RFLP, and the anti-influenza activity conferred by both MX1 isoforms was evaluated in vitro using transfection of Vero cells followed by flow cytometric determination of the fraction of influenza virus-infected cells among MX-producing and MX-nonproducing cell populations. A significant difference in the anti-influenza activity brought by the two MX1 isoforms was demonstrated, which suggests that a significant improvement of innate resistance of pigs by genetic selection might be feasible provided the differences found here in vitro are epidemiologically relevant in vivo.

  15. Genomic structure, promoter analysis, and expression of the porcine (Sus scrofa) Mx1 gene.

    Science.gov (United States)

    Thomas, Anne V; Palm, Melanie; Broers, Aurore D; Zezafoun, Hussein; Desmecht, Daniel J-M

    2006-06-01

    Allelic polymorphisms at the mouse Mx1 locus affect the probability of survival after experimental influenzal disease, raising the possibility that marker-assisted selection using the homologous locus could improve the innate resistance of pigs to natural influenza infections. Several issues need to be resolved before efficient large scale screening of the allelic polymorphism at the porcine (Sus scrofa) Mx1 locus can be implemented. First, the Mx1 genomic structure has to be established and sufficient flanking intronic sequences have to be gathered to enable simple PCR amplification of the coding portions of the gene. Then, a basic knowledge of the promoter region needs to be obtained as an allelic variation there can significantly alter absolute levels and/or tissue-specificity of MX protein expression. The results gathered here show that the porcine Mx1 gene and promoter share the major structural and functional characteristics displayed by their homologs described in cattle, mouse, chicken, and man. The crucial function of the proximal interferon-sensitive response elements motif for gene expression is also demonstrated. The sequence data compiled here will allow an extensive analysis of the polymorphisms present among the widest spectrum possible of porcine breeds with the aim to identify an Mx1 allele providing antiviral resistance.

  16. Cloning and functional characterization of the pig (Sus scrofa) organic anion transporting polypeptide 1a2.

    Science.gov (United States)

    Yu, Yejin; Liu, Xiaoxiao; Zhang, Zheren; Xiao, Yunpeng; Hong, Mei

    2013-08-01

    1. Organic anion transporting polypeptides (OATPs) are a family of transporter proteins that have been extensively recognized as key determinants of absorption, distribution, metabolism and excretion of various drugs. Human OATP1A2 has been demonstrated to transport wide spectrum of endogenous and exogenous compounds. Study on OATP1A2 orthologues of other species, however, is still limited. 2. Here, we described the cloning and functional characterization of a member of the OATP/Oatp family member obtained from pig (Sus scrofa) liver. Sequence analysis suggested that it has a high homology with human OATP1A2 and bovine Oatp1a2. Prototypic substrates estrone-3-sulfate (E-3-S) and taurocholic acid were transported by the protein. The transport of these two substrates is pH-dependent, with lower pH showing higher uptake function. Kinetic study showed the transport of these two substrates have a Km of 42.5 ± 12.1 and 33.1 ± 8.7 µM, respectively. Pig Slco1a2 has the highest expression level in the liver, and to a less extend in the brain and small intestine. 3. In conclusion, an OATP member was cloned from pig liver. Sequence analysis and phylogenic study revealed it as an orthologue of human OATP1A2. Its kinetic characteristic for prototypic substrates and organ distribution are similar with that of OATP1A2.

  17. Organization, complexity and allelic diversity of the porcine (Sus scrofa domestica) immunoglobulin lambda locus.

    Science.gov (United States)

    Schwartz, John C; Lefranc, Marie-Paule; Murtaugh, Michael P

    2012-05-01

    We have characterized the organization, complexity, and expression of the porcine (Sus scrofa domestica) immunoglobulin lambda (IGL) light chain locus, which accounts for about half of antibody light chain usage in swine, yet is nearly totally unknown. Twenty-two IGL variable (IGLV) genes were identified that belong to seven subgroups. Nine genes appear to be functional. Eight possess stop codons, frameshifts, or both, and one is missing the V-EXON. Two additional genes are missing an essential cysteine residue and are classified as ORF (open reading frame). The IGLV genes are organized in two distinct clusters, a constant (C)-proximal cluster dominated by genes similar to the human IGLV3 subgroup, and a C-distal cluster dominated by genes most similar to the human IGLV8 and IGLV5 subgroups. Phylogenetic analysis reveals that the porcine IGLV8 subgroup genes have recently expanded, suggesting a particularly effective role in immunity to porcine-specific pathogens. Moreover, expression of IGLV genes is nearly exclusively restricted to the IGLV3 and IGLV8 genes. The constant locus comprises three tandem cassettes comprised of a joining (IGLJ) gene and a constant (IGLC) gene, whereas a fourth downstream IGLJ gene has no corresponding associated IGLC gene. Comparison of individual BACs generated from the same individual revealed polymorphisms in IGLC2 and several IGLV genes, indicating that allelic variation in IGLV further expands the porcine antibody light chain repertoire.

  18. Radiocesium concentrations in two populations of naturally contaminated feral hogs (Sus scrofa domesticus). [/sup 137/Cs

    Energy Technology Data Exchange (ETDEWEB)

    Stribling, H.L.

    1978-12-01

    Two populations of feral hogs (Sus scrofa domesticus) were studied during the summer and winter seasons from 1976 to 1978 in order to determine concentrations, distributional patterns, and predictability of cesium-137 in the whole body and various body compartments of these animals. One population inhabited the Savannah River Plant (SRP) Aiken, South Carolina, an area contaminated by reactor waste and natural fallout; the other inhabited Ossabaw Island (OI), Georgia, an area contaminated by natural fallout alone. Whole-body burdens on the SRP averaged about 2 and about 1.5 times higher than those samples on OI during the summer and winter seasons, respectively. Radiocesium levels in feral hogs on SRP were not only influenced by the additional source of contamination but apparently fluctuated seasonally as well since the whole-body burdens of SRP hogs sampled were about 1.5 times higher in the summer than in the winter. Levels of cesium-137 in skeletal muscle have decreased in the SRP feral hog population since 1968. On the SRP, feces best predicted whole-body burdens with an r/sup 2/ of 0.67. All internal organs examined predicted levels in skeletal muscle well for SRP animals but best predictability of muscle (R/sup 2/ = 0.94) was achieved when brain, liver, and feces were combined in a multiple regression equation. Muscle was the best predictor of cesium-137 in all body compartments.

  19. Mandibular molar teeth and the development of mastication in the miniature pig (Sus scrofa).

    Science.gov (United States)

    Obrez, A

    1996-01-01

    The only components of the orofacial complex that are fully developed as soon as they establish function are the occlusal surfaces of teeth. It is usually assumed that the occlusal surface of the molar teeth influences the orientation of the power stroke in spite of the fact that the data supporting this claim are lacking. The purpose of this longitudinal study was therefore to determine whether or not this hypothetical form-function relationship existed during development and whether or not it was related to growth. Serial dorsoventral and lateral radiography, and dorsoventral cineradiography were performed during natural feeding of 5 Hanford miniature pigs (Sus scrofa) of both genders between their 8th and 18th weeks. Sequences of power strokes were analyzed frame by frame and compared between sessions, and related to the position of the fourth primary mandibular molar (dm4). The changes in direction of the power stroke and in position of the dm4 relative to the midline were subsequently related to growth. The results of this study indicate that changes in orientation of the power stroke, though significant only on the balancing side, occur independently of the repositioning working and balancing side mandibular molars, as well as of skeletal growth. The null hypothesis that the mandibular primary molars influence the transverse orientation of the power stroke during growth is therefore refuted.

  20. LIMITED ANTIBODY EVIDENCE OF EXPOSURE TO MYCOBACTERIUM BOVIS IN FERAL SWINE (SUS SCROFA) IN THE USA.

    Science.gov (United States)

    Pedersen, Kerri; Miller, Ryan S; Anderson, Theodore D; Pabilonia, Kristy L; Lewis, Jonathan R; Mihalco, Rebecca L; Gortázar, Christian; Gidlewski, Thomas

    2017-01-01

    Bovine tuberculosis is a chronic disease of cattle ( Bos taurus ) caused by the bacterium Mycobacterium bovis . Efforts have been made in the US to eradicate the disease in cattle, but spillover into wildlife and subsequent spillback have impeded progress in some states. In particular, infection in white-tailed deer ( Odocoileus virginianus ) has been followed by infection in cattle in some Midwestern states. Infection has also been documented in feral swine ( Sus scrofa ) on the Hawaiian island of Molokai and in various European countries, but no large-scale survey of antibody exposure to the bacteria has been conducted in feral swine in the US. We tested 488 sera from feral swine collected near previously documented outbreaks of bovine tuberculosis in cattle and captive cervids, in addition to 2,237 feral swine sera collected across the US from 1 October 2013 to 30 September 2014. While all but one of the samples were antibody negative, the results are important for establishing baseline negative data since feral swine are capable reservoirs and could be implicated in future outbreaks of the disease.

  1. 'Post-mortem examination of the reproductive organs of female wild boars (Sus scrofa) in Sweden'.

    Science.gov (United States)

    Malmsten, Anna; Jansson, Gunnar; Dalin, Anne-Marie

    2017-03-13

    In recent decades, wild boars (Sus scrofa) have increased in numbers and distribution in Europe. Compared to other wild ungulates of similar body size, wild boars have a high reproductive capacity. To increase the knowledge of wild boar reproduction, the objective of this study was to investigate characteristics of reproductive organs, and to provide information on the occurrence of abnormalities in reproductive organs from free-ranging female wild boars. Between December 2011 and December 2015, reproductive organs from female wild boars (>30 kg body weight), were collected during hunting in four Swedish counties at estates where supplementary feeding was applied. The organs were macroscopically examined and measured. The stage of the reproductive cycle was defined according to the ovarian structures and in relation to uterus characteristics. Observed abnormalities were noted. The results from 569 animals that met the requirements to be included in this study showed significant differences in weight and length of the uterus between the various reproductive stages. Sampling region had significant effect on these differences. Abnormalities in the reproductive organs were present in approximately 10% of the examined animals. The prevalence of abnormalities increased significantly with age and was significantly affected by sampling region.

  2. Seroprevalence of Toxoplasma gondii in hunted wild boars (Sus scrofa) from southeastern France.

    Science.gov (United States)

    Roqueplo, Cedric; Blaga, Radu; Jean-Lou, Marie; Vallee, Isabelle; Davoust, Bernard

    2017-01-25

    Toxoplasma gondii (Nicolle et Manceaux, 1908) is an obligate intracellular, parasitic protozoan within the phylum Apicomplexa that causes toxoplasmosis in mammalian hosts (including humans) and birds. Since meat of wild boar, Sus scrofa (Linnaeus), has been demonstrated to be a potential source of human infection, a careful evaluation of the prevalence of infection with T. gondii in hunted animals is needed to protect public health. In the Var area in southeastern France, we performed a spatio-temporal survey in order to investigate the prevalence of IgG antibodies in wild boars shot by hunters in the Canjuers military camp during two subsequent hunting seasons. Of 841 wild boars screened, antibodies (IgG) to T. gondii (modified agglutination test, cut-off 1 : 6) were found in 141 (16.8%) muscle extract samples. A significant association (p < 0.001) was found between positivity and age, but not gender, and hunting districts. The results obtained indicate that consumption of raw or undercooked meat from wild boars carries an important risk of infection with T. gondii. Wild boars may be considered as a bioindicator of parasite circulation in this ecosystem.

  3. The complete mitochondrial genome of Juema pig Sus scrofa (Suina: Suidae) from southern Gansu.

    Science.gov (United States)

    Xu, Yan-Yan; Tian, Xiao-Xiao; Chen, Lei-Lei; Pan, Hong-Chun

    2016-09-01

    Juema pig is a kind of rare and special pig which is well adapted to high altitude, cold climate and harsh natural environment. The complete mitochondrial genome of Juema pig Sus scrofa is a circular molecule of 16 532 bp in length, containing 13 protein-coding genes, two ribosomal RNAs, 22 transfer RNAs, and a control region. The A + T content of the overall base composition of H-strand is 60.7% (T: 26.2%; C: 26.0%; A: 34.5%; G: 13.3%). ND4L gene begins with GTG as start codon, ND2, ND3, and ND5 genes begin with ATA as a start codon, and other nine protein-coding genes start with ATG. Cyt b gene is terminated with AGA as stop codon, ND1 and ND2 genes are terminated with TAG as stop codon, COII, COIII, ND3, and ND4 end with T, while ATP6, ATP8, COI, ND4L, ND5, and ND6 end with TAA. In addition, the phylogenetic relationships from neighbor-joining analyses based on the 13 concatenated PCGs indicated (Tylopoda (Suina (Ruminantia (Hippopotamidae, Cetacea)))).

  4. Evaluation of different soil parameters and wild boar (Sus scrofa [L.] grassland damage

    Directory of Open Access Journals (Sweden)

    Žiga Laznik

    2014-10-01

    Full Text Available Presented in this paper are the correlations between different soil parameters [presence of grubs, earthworms, pH, content of P2O5, K2O and organic matter (OM in soil] and wild boar (Sus scrofa [L.] damage to grasslands. The soil samples and damage assessments were performed at six locations in the Kočevje region, which is a densely wooded part of South East Slovenia. A significant positive correlation was discovered between the extent of damage due to wild boar rooting in grasslands and the number of grubs (r=0.73, the weight of grubs (r=0.69 and the content of P2O5 (r=0.87 in the soil. The quantity and weight of grubs in soil were significantly influenced by soil pH, the content of CaCl2 (r=0.71/0.72, P2O5 (r=0.90/0.91, and OM (r=0.74/0.77; while the quantity and weight of earthworms in soil were influenced by the content of K2O (r=0.81/-0.84. A moderate yet insignificant correlation (r=0.48/0.56 was discovered between the number and weight of earthworms in soil and the extent of grassland damage. Grubs represent a more important source of protein for wild boars than earthworms; consequently, reducing the quantity of grubs in soil could minimise the extent of damage caused by boars.

  5. Snaring to control feral pigs sus scrofa in a remote Hawaiian rain forest

    Science.gov (United States)

    Anderson, Stephen J.; Stone, Charles P.

    1993-01-01

    Feral pig Sus scrofa control in Kipahulu Valley, a remote rain forest in Haleakala National Park, Maui, Hawaiian Islands, has been achieved with snares over a 45-month period. Initial pig densities in fenced management units of 6·2 km2 and 7·8 km2were estimated at 6 animals/km2 and 14·3 animals/km2 for the two units, based on population reconstruction from animals killed and aged. During the 45 months of the study, 1978 snares were set, and 1·6 million snare nights were logged. Snare density reached 96/km2 and 200/km2 for the two management units by the end of the study. A mean effort of 43 worker hours/pig was used to remove 53 pigs from the upper management unit, and a mean of 7 worker hours/pig to remove 175 animals from the more densely populated lower unit. Pig activity monitoring along transects provided a good measure of control effectiveness until densities of about 1 pig/km2 were achieved, after which transects became less useful than scouting for determining pig activity.

  6. Serologic and molecular survey for hepatitis E virus in wild boar (Sus scrofa) in Central Italy.

    Science.gov (United States)

    Mazzei, M; Nardini, R; Verin, R; Forzan, M; Poli, A; Tolari, F

    2015-09-01

    The aim of this study was to further investigate the role of wild boar (Sus scrofa) as a reservoir for hepatitis E virus (HEV). Sixty-four blood and faecal samples collected from wild boar hunted in Central Italy in 2011-2012 were examined by indirect enzyme-linked immunosorbent assay and RT-PCR analysis. Positive RT-PCR samples were further examined by nucleotide sequence determination and subsequent phylogenetic analysis. Thirty-six sera (56.2%) were positive for HEV-specific antibodies, and six (9.4%) faecal samples scored RT-PCR-positive results. Four animals were positive by both enzyme-linked immunosorbent assay and RT-PCR. Phylogenetic analysis showed that the detected wild boar-derived HEV sequences clustered within genotype 3, with similarity to sequences of human origin collected in a nearby area in 2012. Our data confirm that HEV is endemic in the wild boar population in the research area and that these wild animals could play an important role in the epidemiology of HEV infection.

  7. Cholinergic profiles in the Goettingen miniature pig (Sus scrofa domesticus) brain.

    Science.gov (United States)

    Mahady, Laura J; Perez, Sylvia E; Emerich, Dwaine F; Wahlberg, Lars U; Mufson, Elliott J

    2017-02-15

    Central cholinergic structures within the brain of the even-toed hoofed Goettingen miniature domestic pig (Sus scrofa domesticus) were evaluated by immunohistochemical visualization of choline acetyltransferase (ChAT) and the low-affinity neurotrophin receptor, p75(NTR) . ChAT-immunoreactive (-ir) perikarya were seen in the olfactory tubercle, striatum, medial septal nucleus, vertical and horizontal limbs of the diagonal band of Broca, and the nucleus basalis of Meynert, medial habenular nucleus, zona incerta, neurosecretory arcuate nucleus, cranial motor nuclei III and IV, Edinger-Westphal nucleus, parabigeminal nucleus, pedunculopontine nucleus, and laterodorsal tegmental nucleus. Cholinergic ChAT-ir neurons were also found within transitional cortical areas (insular, cingulate, and piriform cortices) and hippocampus proper. ChAT-ir fibers were seen throughout the dentate gyrus and hippocampus, in the mediodorsal, laterodorsal, anteroventral, and parateanial thalamic nuclei, the fasciculus retroflexus of Meynert, basolateral and basomedial amygdaloid nuclei, anterior pretectal and interpeduncular nuclei, as well as select laminae of the superior colliculus. Double immunofluorescence demonstrated that virtually all ChAT-ir basal forebrain neurons were also p75(NTR) -positive. The present findings indicate that the central cholinergic system in the miniature pig is similar to other mammalian species. Therefore, the miniature pig may be an appropriate animal model for preclinical studies of neurodegenerative diseases where the cholinergic system is compromised. J. Comp. Neurol. 525:553-573, 2017. © 2016 Wiley Periodicals, Inc.

  8. Selected Biometric Characteristics of Wild Boar (Sus Scrofa Ferus in North-East Romania

    Directory of Open Access Journals (Sweden)

    Alina Narcisa Postolache

    2015-05-01

    Full Text Available Our study analyzed selected biometric characteristics from 117 wild boars (Sus scrofa ferus harvested during 2008 – 2014 in the Frasin and Marginea Forest hunting ground districts of Suceava County. Hunted boars were measured individually for head-body length (cm, height at withers (cm, length of metatarsus (cm, ear length (cm, tail length (cm and body weight (kg in accordance with their age-class and gender. These characteristics give information on the growth and development of wild boars and on the quality of their habitat. It was found that the average carcass weight was: piglets – 28.4 kg, yearling – 78.1 kg, subadults – 102.9 kg. The results show a faster body growth in females during their first year, while males make up for the weight difference in their 2nd and 3rd year. Statistical differences shown that males differentiate significantly to females by weight, body length, height at withers and length of metatarsus (P < 0.05 starting with their second year of life. The results regarding growth dynamic go along with the changes in boar’s social life, when the males are forced to leave and form smaller groups.

  9. Developmental changes affecting lectin binding in the vomeronasal organ of domestic pigs, Sus scrofa.

    Science.gov (United States)

    Park, Junwoo; Lee, Wonho; Jeong, Chanwoo; Kim, Hwangryong; Taniguchi, Kazumi; Shin, Taekyun

    2012-01-01

    This study investigated the developmental changes of glycoconjugate patterns in the porcine vomeronasal organs (VNOs) and associated glands (Jacobson's glands) from prenatal (9 weeks of gestation) and postnatal (2 days after birth) to the sexually mature stage (6 months old). The VNO of pigs (Sus scrofa) was examined using the following: Dolichos biflorus agglutinin (DBA), Bandeiraea simplicifolia agglutinin isolectin B4 (BSI-B4), Triticum vulgaris agglutinin (WGA), Ulex europaeus agglutinin I (UEA-I), and soybean agglutinin (SBA). At the fetal stage, all lectins examined were detected mainly in the free border of the vomeronasal epithelium, but few (WGA and UEA-I) and or absent in the VNO cell bodies. At the postnatal and sexually mature stages, the reactivity of some lectins, including WGA, UEA-I, DBA and SBA, were shown to increase in the VNO sensory epithelium as well as the free border. The increased reactivity of lectins as development progressed was also observed in Jacobson's gland acini. These findings suggest that binding sites of lectins, including those of WGA, UEA-I, DBA, and SBA, increase during development from fetal to postnatal growth, possibly contributing to the increased ability of chemoreception in the pig.

  10. Isolation and characterization of a novel Rhabdovirus from a wild boar (Sus scrofa) in Japan.

    Science.gov (United States)

    Sakai, Kouji; Hagiwara, Katsuro; Omatsu, Tsutomu; Hamasaki, Chinami; Kuwata, Ryusei; Shimoda, Hiroshi; Suzuki, Kazuo; Endoh, Daiji; Nagata, Noriyo; Nagai, Makoto; Katayama, Yukie; Oba, Mami; Kurane, Ichiro; Saijo, Masayuki; Morikawa, Shigeru; Mizutani, Tetsuya; Maeda, Ken

    2015-09-30

    A novel rhabdovirus was isolated from the serum of a healthy Japanese wild boar (Sus scrofa leucomystax) and identified using the rapid determination system for viral nucleic acid sequences (RDV), next-generation sequencing, and electron microscopy. The virus was tentatively named wild boar rhabdovirus 1 (WBRV1). Phylogenetic analysis of the entire genome sequence indicated that WBRV1 is closely related to Tupaia rhabdovirus (TRV), which was isolated from cultured cells of hepatocellular carcinoma tissue of tree shrew. TRV has not been assigned to any genus of Rhabdoviridae till date. Analysis of the L gene indicated that WBRV1 belongs to the genus Vesiculovirus. These observations suggest that both TRV and WBRV1 belong to a new genus of Rhabdoviridae. Next-generation genome sequencing of WBRV1 revealed 5 open reading frames of 1329, 765, 627, 1629, and 6336 bases in length. The WBRV1 gene sequences are similar to those of other rhabdoviruses. Epizootiological analysis of a population of wild boars in Wakayama prefecture in Japan indicated that 6.5% were positive for the WBRV1 gene and 52% were positive for WBRV1-neutralizing antibodies. Furthermore, such viral neutralizing antibodies were found in domestic pigs in another prefecture. WBRV1 was inoculated intranasally and intraperitoneally into SCID and BALB/c mice and viral RNA was detected in SCID mice, suggesting that WBRV1 can replicate in immunocompromised mice. These results indicate this novel virus is endemic in wild animals and livestock in Japan.

  11. Exposure of feral swine (Sus scrofa) in the United States to selected pathogens.

    Science.gov (United States)

    Baroch, John A; Gagnon, Carl A; Lacouture, Sonia; Gottschalk, Marcelo

    2015-01-01

    Feral swine (Sus scrofa) are widely distributed in the United States. In 2011 and 2012, serum samples and tonsils were recovered from 162 and 37 feral swine, respectively, in the US to evaluate exposure to important swine endemic pathogens. Antibodies against porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) were found in 2.5% and 25.3% of tested sera, respectively. Positive serological reactions against Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae have been detected in 19.7% and 69.7% of animals. More than 15% of animals presented antibodies against these 2 pathogens simultaneously. Most animals were also seropositive for Lawsonia intracellularis. Feral swine can also be involved in transmission of zoonotic agents. Almost 50% of animals possessed antibodies against Salmonella. In addition, 94.4% of animals were carriers of Streptococcus suis in their tonsils. In conclusion, feral swine may be considered as a potential reservoir for different endemic diseases in domestic pigs, as well as for important zoonotic agents.

  12. Spatiotemporal and Ecological Patterns of Mycobacterium microti Infection in Wild Boar (Sus scrofa).

    Science.gov (United States)

    Chiari, M; Ferrari, N; Giardiello, D; Avisani, D; Pacciarini, M L; Alborali, L; Zanoni, M; Boniotti, M B

    2016-10-01

    Mycobacterium microti has recently been described as the causative agent of tuberculosis-like lesions in wild boar (Sus scrofa), a reservoir specie of Mycobacterium tuberculosis complex (MTBC) in some European Mediterranean ecosystem. Through a five-year survey on tuberculosis in free-living wild boars, the epidemiological trend of M. microti infections and the host and population risk factors linked with its occurrence were described. Retropharyngeal and mandibular lymph nodes of 3041 hunted wild boars from six different districts were macroscopically inspected. The sex and age of each animal were registered, as well as the animal abundance in each district. Lesions compatible with tuberculosis (190) were collected and analysed using a gyrB PCR-RFLP assay. M. microti was identified directly in 99 tissue samples (Prev = 3.26%; 95% CI: 2.67-3.97%), while neither Mycobacterium bovis, nor other members of the MTBC were detected. The probability of being M. microti positive showed spatio-temporal variability, with 26% of increase of risk of being infected for each year. Moreover, a positive effect of wild boar abundance and age on the prevalence was detected. The generalized increase in the European wild boar population, coupled with its sensitivity to M. microti infection, poses a future concern for the identification and management of MTBC members in wild boar.

  13. Mitochondrial DNA sequence and phylogenetic evaluation of geographically disparate Sus scrofa breeds.

    Science.gov (United States)

    Cannon, M V; Brandebourg, T D; Kohn, M C; Ðikić, D; Irwin, M H; Pinkert, C A

    2015-01-01

    Next generation sequencing of mitochondrial DNA (mtDNA) facilitates studies into the metabolic characteristics of production animals and their relation to production traits. Sequence analysis of mtDNA from pure-bred swine with highly disparate production characteristics (Mangalica Blonde, Mangalica Swallow-bellied, Meishan, Turopolje, and Yorkshire) was initiated to evaluate the influence of mtDNA polymorphisms on mitochondrial function. Herein, we report the complete mtDNA sequences of five Sus scrofa breeds and evaluate their position within the phylogeny of domestic swine. Phenotypic traits of Yorkshire, Mangalica Blonde, and Swallow-belly swine are presented to demonstrate their metabolic characteristics. Our data support the division of European and Asian breeds noted previously and confirm European ancestry of Mangalica and Turopolje breeds. Furthermore, mtDNA differences between breeds suggest function-altering changes in proteins involved in oxidative phosphorylation such as ATP synthase 6 (MT-ATP6), cytochrome oxidase I (MT-CO1), cytochrome oxidase III (MT-CO3), and cytochrome b (MT-CYB), supporting the hypothesis that mtDNA polymorphisms contribute to differences in metabolic traits between swine breeds. Our sequence data form the basis for future research into the roles of mtDNA in determining production traits in domestic animals. Additionally, such studies should provide insight into how mtDNA haplotype influences the extreme adiposity observed in Mangalica breeds.

  14. Prevalence of Leptospira antibodies in wild boars (Sus scrofa) from Northern Portugal: risk factor analysis.

    Science.gov (United States)

    Vale-Gonçalves, H M; Cabral, J A; Faria, M C; Nunes-Pereira, M; Faria, A S; Veloso, O; Vieira, M L; Paiva-Cardoso, Md N

    2015-07-01

    Leptospirosis is a zoonosis of worldwide distribution, caused by infection with pathogenic spirochaetes of the genus Leptospira. The wild boar (Sus scrofa), an important hunting species in Europe, seems to play a significant role in the epidemiological cycle of leptospirosis. A total of 101 serum samples from wild boar hunted in Northern Portugal were analysed for leptospiral antibodies detection by microscopic agglutination test. Sera were collected during hunting seasons (2011-2013) and tested with 17 different pathogenic serovars of Leptospira. Antibodies against nine serovars were detected in 66 (65·4%) of these sera. Serovars Tarassovi and Altodouro exhibited the highest seroreactivity rates (23·8% and 16·8%, respectively), followed by Autumnalis (7·9%) and Bratislava (6·9%). Age and district of origin were found to be risk factors for the presence of leptospiral antibodies in contrast to gender. From a One Health perspective, this study revealed that wild boar should be considered as a potential source of leptospirosis dissemination for humans and animal species (domestic and wild) in shared environments, particularly in the Trás-os-Montes region.

  15. Establishing a DNA identification system for pigs (Sus scrofa) using a multiplex STR amplification.

    Science.gov (United States)

    Lin, Yu-Chih; Hsieh, Hsing-Mei; Lee, James Chun-I; Hsiao, Chung-Ting; Lin, Der-Yuh; Linacre, Adrian; Tsai, Li-Chin

    2014-03-01

    In this study we establish a novel STR multiplex using 13 tetra-nucleotide STRs and the amelogenin marker for the forensic identification of pigs. The genotypes and allele frequency were generated based on 341 samples from 11 pig breeds in Taiwan. Genetic variation was tested including Na, Ne, Ho, He, F-statistics, PIC, Pm and PE for each STR locus and for each breed. Based upon the 341 samples in this study, the CPm and CPEtrio of the 13 STR loci were 1.31 E-11 and 0.9996 respectively. The CPItrio based on ten family sets ranged from 4.012 E+4 to 4.332 E+6 for paternity test. Validation of the multiplex included: determining the sensitivity of the test, where reproducible full DNA profiles were obtained using an initial template of between 0.25 and 1 ng; a comprehensive range of tissue types generated the same genotype; and the specificity was confirmed as no DNA full profile was generated for any species other than Sus scrofa. Based on the phylogenetic analysis, the European domestic breeds clustered separately from the Asian breeds, as expected, and their hybrids formed unique clades respectively between the clades of Asian and European breeds. Eleven test samples, acting as unknown samples, matched all expected breeds. We demonstrate that this novel 14-plex PCR system is valuable in pig individualization, parentage testing, breed assessment, phylogenetic study and forensic applications.

  16. Experimental taphonomy: post-mortem microstructural modifications in Sus scrofa domesticus bone.

    Science.gov (United States)

    Kontopoulos, Ioannis; Nystrom, Pia; White, Lorraine

    2016-09-01

    Bone is a highly specialised form of hard and rigid connective tissue whose histological structure undergoes post-mortem modifications. In taphonomic research, histological examination of bone thin sections is used to investigate these post-mortem microstructural changes in skeletal tissues. In this study, diagenetic modifications in pig skeletal remains (Sus scrofa domesticus) which were exposed to different taphonomic conditions as part of a long-term, real-time experiment were examined under light microscope (i.e. plain and cross polarized light). This experiment demonstrated that macroscopic appearance and microscopic preservation of bone may significantly differ. Early microbial attack was identified as enlarged osteocyte lacunae that later coalesce to constitute larger foci. Additionally, microscopic preservation of different skeletal elements varied intra-individually, while within bone differential preservation (i.e. proximal versus distal ends) was also observed. However, no specific patterns of early histological attack (e.g. endosteal and periosteal destruction) and no clear relationship between histological preservation and proximity to the abdominal area were detected. Lastly, the presence and composition of protective textiles had a clear effect on bone preservation. This research project, therefore, provided important evidence for the better understanding of the diagenetic processes that occur within bones whilst buried or exposed on the ground surface.

  17. Identification and antimicrobial resistance of microflora colonizing feral pig (Sus scrofa) of Brazilian Pantanal.

    Science.gov (United States)

    Lessa, Ss; Paes, Rcs; Santoro, Pn; Mauro, Ra; Vieira-da-Motta, O

    2011-04-01

    Antimicrobial resistance of bacteria is a worldwide problem affecting wild life by living with resistant bacteria in the environment. This study presents a discussion of outside factors environment on microflora of feral pigs (Sus scrofa) from Brazilian Pantanal. Animals had samples collected from six different body sites coming from two separated geographic areas, Nhecolandia and Rio Negro regions. With routine biochemical tests and commercial kits 516 bacteria were identified, with 240 Gram-positive, predominantly staphylococci (36) and enterococci (186) strains. Among Gram-negative (GN) bacteria the predominant specimens of Enterobacteriaceae (247) mainly represented by Serratia spp. (105), Escherichia coli (50), and Enterobacter spp. (40) and specimens not identified (7). Antimicrobial susceptibility was tested against 17 drugs by agar diffusion method. Staphylococci were negative to production of enterotoxins and TSST-1, with all strains sensitive towards four drugs and highest resistance toward ampicillin (17%). Enterococci presented the highest sensitivity against vancomycin (98%), ampicillin (94%) and tetracycline (90%), and highest resistance pattern toward oxacillin (99%), clindamycin (83%), and cotrimoxazole (54%). In GN the highest resistance was observed with Serratia marcescens against CFL (98%), AMC (66%) and AMP (60%) and all drugs was most effective against E. coli SUT, TET (100%), AMP, TOB (98%), GEN, CLO (95%), CFO, CIP (93%). The results show a new profile of oxacillin-resistant enterococci from Brazilian feral pigs and suggest a limited residue and spreading of antimicrobials in the environment, possibly because of low anthropogenic impact reflected by the drug susceptibility profile of bacteria isolated.

  18. Genetic characterization and phylogeography of the wild boar Sus scrofa introduced into Uruguay

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    Graciela García

    2011-01-01

    Full Text Available The European wild boar Sus scrofa was first introduced into Uruguay, in southern South America during the early decades of the last century. Subsequently, and starting from founder populations, its range spread throughout the country and into the neighbouring Brazilian state Rio Grande do Sul. Due to the subsequent negative impact, it was officially declared a national pest. The main aim in the present study was to provide a more comprehensive scenario of wild boar differentiation in Uruguay, by using mtDNA markers to access the genetic characterization of populations at present undergoing rapid expansion. A high level of haplotype diversity, intermediate levels of nucleotide diversity and considerable population differentiation, were detected among sampled localities throughout major watercourses and catchment dams countrywide. Phylogenetic analysis revealed the existence of two different phylogroups, thereby reflecting two deliberate introduction events forming distantly genetic lineages in local wild boar populations. Our analysis lends support to the hypothesis that the invasive potential of populations emerge from introgressive hybridization with domestic pigs. On taking into account the appreciable differentiation and reduced migration between locales in wild boar populations, management strategies could be effective if each population were to be considered as a single management unit.

  19. Neospora caninum exposure in overlapping populations of coyotes (Canis latrans) and feral swine (Sus scrofa).

    Science.gov (United States)

    Bevins, Sarah; Blizzard, Emily; Bazan, Luis; Whitley, Pat

    2013-10-01

    Limited information exists on Neospora caninum transmission dynamics in wildlife. This coccidian parasite, whose presence can lead to substantial economic losses in cattle operations, requires a canid definitive host for reproduction. We examined exposure in a definitive host, coyotes (Canis latrans), and in overlapping populations of feral swine (Sus scrofa) to determine if spatial proximity between a definitive and incidental host influences the likelihood of parasite exposure. Eighteen percent of coyotes (95% confidence interval [CI] = 14.2-21.8) and 15.8% of feral swine (95% CI = 12.5-19.2) had been exposed to N. caninum, and this is the first report of exposure in US feral swine populations. Analyses suggest that the parasite is present throughout the environment and that exposure is not temporally or spatially linked to antibody-positive coyotes. Antibody-positive feral swine were found in an area where the only definitive host is domestic dogs (Canis familiaris), indicating that wild canids are not required to maintain the parasite in the environment.

  20. Identification and antimicrobial resistance of microflora colonizing feral pig (Sus scrofa of Brazilian Pantanal

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    SS Lessa

    2011-06-01

    Full Text Available Antimicrobial resistance of bacteria is a worldwide problem affecting wild life by living with resistant bacteria in the environment. This study presents a discussion of outside factors environment on microflora of feral pigs (Sus scrofa from Brazilian Pantanal. Animals had samples collected from six different body sites coming from two separated geographic areas, Nhecolandia and Rio Negro regions. With routine biochemical tests and commercial kits 516 bacteria were identified, with 240 Gram-positive, predominantly staphylococci (36 and enterococci (186 strains. Among Gram-negative (GN bacteria the predominant specimens of Enterobacteriaceae (247 mainly represented by Serratia spp. (105, Escherichia coli (50, and Enterobacter spp. (40 and specimens not identified (7. Antimicrobial susceptibility was tested against 17 drugs by agar diffusion method. Staphylococci were negative to production of enterotoxins and TSST-1, with all strains sensitive towards four drugs and highest resistance toward ampicillin (17%. Enterococci presented the highest sensitivity against vancomycin (98%, ampicillin (94% and tetracycline (90%, and highest resistance pattern toward oxacillin (99%, clindamycin (83%, and cotrimoxazole (54%. In GN the highest resistance was observed with Serratia marcescens against CFL (98%, AMC (66% and AMP (60% and all drugs was most effective against E. coli SUT, TET (100%, AMP, TOB (98%, GEN, CLO (95%, CFO, CIP (93%. The results show a new profile of oxacillin-resistant enterococci from Brazilian feral pigs and suggest a limited residue and spreading of antimicrobials in the environment, possibly because of low anthropogenic impact reflected by the drug susceptibility profile of bacteria isolated.

  1. Current distribution and population status of wild boar (Sus scrofa L. in Greece

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    TSACHALIDIS, Efstathios P.

    2009-01-01

    Full Text Available This is the first attempt to estimate wild boar (Sus scrofa L. population density anddistribution in Greece. The study took place in 2004, in all 38 Greek counties of the mainland, as wellas in the larger forested Greek islands: Crete, Rodos, Samos, Lesvos, Limnos, Thasos, Corfu,Kefalonia and Zakinthos. It was observed that the species was present in every county in continentalGreece, with the exception of the two counties Attica and Evia. Attica, which includes the capital cityof Athens, is densely inhabited not allowing much space for wildlife. Evian is an island connected tothe mainland with a bridge. Wild boar can be found in an area around 19,495 km2, which is about 14%of the country. The mean distribution altitude reaches 686 ± 266 m. The average population numberwas estimated at 19,033 (0.98 ± 0, 21 ind/km2 individuals with maximum population level 23,030animals, and a minimum of 16,536. The highest mean density was observed in the prefecture of StereaHellas (1.26 ind/km2 and the lowest in the prefecture of Thrace (0.89 ind/km2. The maximum densitywas found in Sterea Hellas (3.14 ind/km2 and the minimum density in Thrace (0.13 ind/km2. Theabove results are expected to provide valuable information for the management of wild boar in Greece.

  2. Morphometrical Analysis of Reproduction Traits for the Wild Boar (Sus scrofa L. in Croatia

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    Nikica Šprem

    2011-09-01

    Full Text Available The wild boar (Sus scrofa L. is native game in Croatia, whose population have tendency of increasing as well throughout the Europe. The wild boar is a natural inhabitant of Europe, Asia, and North Africa and is phylogenetically the ancestor of the domestic pig. Because of its phylogenetic and economic importance, this species is an interesting model for studying testis function. Therefore, the present study was performed to investigate the testis morphometry, and gonadosomatic index (GSI for 77 individuals. The mean live body weight was 75.03 kg, testis weight was 0.355 kg and with a gonadosomatic index (GSI of approximately 0.40%. The mean circumference for the left and right testes were not significant, but a significant and positive correlation was observed between testis weight and body weight (r = 0.88, p<0.05. A high reproductive contribution of juveniles is a likely consequence of a high hunting pressure rather than a species specific life history pattern characterizing wild boar. Generally, beside female seasonal reproductive activity knowledge of male reproduction cycle in wild boar is very important for established better management of free-ranging population.

  3. A Comparison of Proximal Tibia and Proximal Humerus Infusion Rates of Plasma Under High Pressure Using the EZ IO Intraosseous Device in the Adult Swine (Sus scrofa) Hypovolemic Model

    Science.gov (United States)

    2013-01-10

    plasma under high pressure using the EZ IO intraosseous device in the adult swine (Sus scrofa ) hypovolemic model. FWH20100171A FWH20100171ALairet...resuscitation, swine, sus scrofa U U U SAR Vikhyat Bebarta, MD 210-275-3794 WHASC- Animal Final Report 170ct2012 1. Protocol Number: FWH20100171A 2...the EZ 10 intraosseous device in the adult swine (Sus scrofa ) hypovolemic model. 4. Principal Investigator PI): Name Rank Date of Branch Staff

  4. A Comparison of Proximal Tibia, Proximal Humerus and Distal Femur Infusion Rates of Blood Under High Pressure Using the EX IO Intraosseous Device in the Adult Swine (Sus scrofa) Hypovolemic Model

    Science.gov (United States)

    2012-07-25

    of Blood Under High Pressure Using the EZ IO Intraosseous Device on the Adult Swine (Sus scrofa ) hypovolemic Model.” 4. Principal Investigator...femur infusion rates of blood under high pressure using the EZ 10 intraosseous device in the 00. UI1AIIII IIIUIVII:IC:I1 adult swine (Sus scrofa ...hypovolemic (sus scrofa ). METHOD:3 groups of animals; 14 per group were intubated and ventilated. Central vein and arterjallines were placed, blood

  5. Juvenile domestic pigs (Sus scrofa domestica) use human-given cues in an object choice task.

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    Nawroth, Christian; Ebersbach, Mirjam; von Borell, Eberhard

    2014-05-01

    Research on the comprehension of human-given cues by domesticated as well as non-domesticated species has received considerable attention over the last decade. While several species seem to be capable of utilizing these cues, former work with domestic pigs (Sus scrofa domestica) has shown inconclusive results. In this study, we investigated the use of human-given cues in an object choice task by young domestic pigs (N = 17; 7 weeks of age) who had very limited human contact prior to the experiments. Subjects had to choose between two bowls of which only one was baited with a reward. Over the course of five experiments, pigs were able to use proximal and, with some constraints, also distal pointing cues presented in both a dynamic-sustained and in a momentary manner. When the experimenter was pointing from the incorrect bowl towards the correct one, most of the subjects had problems solving the task-indicating that some form of stimulus/local enhancement affected pigs' decision making. Interestingly, pigs were able to utilize the body and head orientation of a human experimenter to locate the hidden reward but failed to co-orient when head or body orientation of the experimenter was directed into distant space with no bowls present. Control trials ruled out the possibility that other factors (e.g. odour cues) affected subjects' choice behaviour. Learning during experiments played a minor role and only occurred in three out of twelve test conditions. We conclude that domestic pigs, even at a very young age, are skilful in utilizing various human-given cues in an object choice task-raising the question whether pigs only used stimulus/local enhancement and associative learning processes or whether they were able to comprehend the communicative nature of at least some of these cues.

  6. Helminth Parasites of Wild Boars, Sus scrofa, in Bushehr Prov-ince, Southwestern Iran

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    Majid MANSOURI

    2016-10-01

    Full Text Available Background: Wild boars, Sus scrofa, of wide distribution considered as a potential source of zoonotic parasites. The current study aimed to assess the prevalence of helminth infections in wild boars in the Persian Gulf coastal area (Bushehr Province, Southwestern Iran.Methods: Twenty-five wild boars, including 11 males and 14 females, were collected during a course of vertebrate pest control in the Bushehr Province, southwestern Iran in 2013. The specimen were immediately dissected and carefully searched for the parasites. During necropsy, each organ was examined macroscopically for presence of any helminthic agents. Tissue samples were taken from each organ. Moreover, samples were taken from the content of digestive system. Blood samples were also collected from each boar. All the samples were evaluated for helminth infections by parasitological methods.Results: Twenty-two (88% of the wild boars were infected with at least one helminth. Out of 25 wild boars, 1 (4% were infected with Cysticercus tenuicollis, the larval stage of Taenia hydatigena, 13 (52% with Macracanthorhynchus hirudinaceus, 17 (68% with Metastrongylus spp, and 20 (80% with Ascarops spp. Hydatid cyst was detected in the lung of one of the wild boars. No Trichinella spp. larvae were detected in any of the tissues of the animals when evaluated by artificial digestion method. In addition, no contamination with microfilaria was detected in any of animals when the blood samples were tested with Knott’s method.Conclusion: Wild boars are contaminated by some helminthes including zoonotic ones. These animals could be involved in the epidemiology of zoonotic helminth by acting as reservoir hosts. This in turn may bring potential risk for locals and residents of the Bushehr Province, Southwestern Iran.

  7. Ultra structure of the denervated vocal muscle mechanically in hogs (sus scrofa domestica

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    Leão, Henrique Zaquia

    2010-03-01

    Full Text Available Introduction: The literature is not clear in the ultra-structural manifestations of the vocal wrinkles after neural wound. Objective: To verify the alterations that occur in a vocal fold mechanically denervated. Method: In this prospective study, it were utilized 15 hogs of commercial race (Sus scrofa domesticates, with age of 4 to 12 weeks. The animals were distributed in three groups, chosen at random. Everybody was submitted to the denervation of the right vocal fold, with surgical removal of a segment with three centimeters of the recurring right laryngeal nerve. After 45, 90 and 180 days of the operations, it was proceeded the biopsy of the vocal muscles, it was prosecuted the samples for transmission electron microscopy and, for the ultra-structural study, utilized the transmission electron microscopy Philips, model EM208S. Results: The biopsied groups with 45 and 90 days after operation of mechanical denervation, presented disorganization miofibrilar, only vestigial lines Z in many samples, as well like altered mithochondrions presenting limited sizes, and matrix mithocondrial rarefied with rare mithocondrial cristae present. The biopsied group with 180 days after operation of denervation, presented regular sarcomeres, mithocondrions with sizes and regular number with correct positioning between the sarcomerical units. Conclusion: The finds in the ultra-structure of the vocal muscles suggest to re enervation of the muscle being that the muscular mithochondrions were the most sensible structures to the denervated condition, successions by the cytoarchiteture of the miofibrilas; the finds in the ultra-structure of the vocal muscles suggests to reinervation of the muscle in the period of approximately six months.

  8. Gene expression profile suggests that pigs (Sus scrofa are susceptible to Anaplasma phagocytophilum but control infection

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    Galindo Ruth C

    2012-08-01

    Full Text Available Abstract Background Anaplasma phagocytophilum infects a wide variety of hosts and causes granulocytic anaplasmosis in humans, horses and dogs and tick-borne fever in ruminants. Infection with A. phagocytophilum results in the modification of host gene expression and immune response. The objective of this research was to characterize gene expression in pigs (Sus scrofa naturally and experimentally infected with A. phagocytophilum trying to identify mechanisms that help to explain low infection prevalence in this species. Results For gene expression analysis in naturally infected pigs, microarray hybridization was used. The expression of differentially expressed immune response genes was analyzed by real-time RT-PCR in naturally and experimentally infected pigs. Results suggested that A. phagocytophilum infection affected cytoskeleton rearrangement and increased both innate and adaptive immune responses by up regulation of interleukin 1 receptor accessory protein-like 1 (IL1RAPL1, T-cell receptor alpha chain (TCR-alpha, thrombospondin 4 (TSP-4 and Gap junction protein alpha 1 (GJA1 genes. Higher serum levels of IL-1 beta, IL-8 and TNF-alpha in infected pigs when compared to controls supported data obtained at the mRNA level. Conclusions These results suggested that pigs are susceptible to A. phagocytophilum but control infection, particularly through activation of innate immune responses, phagocytosis and autophagy. This fact may account for the low infection prevalence detected in pigs in some regions and thus their low or no impact as a reservoir host for this pathogen. These results advanced our understanding of the molecular mechanisms at the host-pathogen interface and suggested a role for newly reported genes in the protection of pigs against A. phagocytophilum.

  9. Habitat use by wild boar Sus scrofa in Moncayo Nature Park, Spain

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    Rodrigues, Patrícia

    2016-12-01

    Full Text Available Habitat use by wild boar Sus scrofaz was examined during a three-year period in Moncayo Nature Park, a protected mountain area in the Iberian mountain system, Spain. Tracking indirect signs of activity was used to collect data on the species occurrence, according to vegetation type, topography, hunting activity, and season. The data were analysed using binary logistic regression. Habitat used by wild boar differed according seasons, management practices, and vegetation. Main selected habitats were at medium elevations (1,101-1,600 m in areas dominated by holm oak (Quercus ilex, beech (Fagus sylvatica and oak woods of Q. robur, Q. petraea and Q. pyrenaica. Non-hunting areas were selected over hunting areas. We found a seasonal variation in the habitat use of wild boar, with areas dominated by holm oak being used disproportionately in spring, and areas at medium elevations selected only during summer. The results also support the view that non-hunting areas provide a refuge for this species inside the protected area.Estudiamos el uso del hábitat por parte del jabalí Sus scrofa a lo largo de tres años en el Parque Natural del Moncayo, un área protegida de montaña en el Sistema Ibérico, España. Para ello rastreamos las huellas y señales de su actividad en función de la vegetación, topografía, actividad cinegética y estacionalidad. Los datos fueron analizados utilizando regresiones logísticas binarias. El hábitat usado por el jabalí difiere según las estaciones, gestión y vegetación. Los hábitat mayoritariamente seleccionados fueron las altitudes medias (1101-1600 m en áreas dominadas por la encina (Quercus ilex, haya (Fagus sylvatica y robles (Q. robur, Q. petraea y Q. pirenaica. Las zonas no cinegéticas fueron seleccionadas frente a las cinegéticas. Encontramos diferencias estacionales en el uso del hábitat, con un uso mayor al esperado de los encinares en primavera así como de altitudes medias durante el verano. Los

  10. Preliminary study of molecular variability for neolithic pig (sus scrofa domesticus from romania using the cytochrome b

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    Lucian Gorgan

    2010-06-01

    Full Text Available The aim of the present study is to reveal molecular differences between old and actual populations of pig (Sus scrofa domesticus, based on the analysis of hyper variable first part of cytochrome b. Neolithic pig remains (bone fragments are sampled from Poduri-Dealul Ghindaru Tell (Bacau County. The Tell of Poduri-Dealul Ghindaru (positioned in the Eastern part of Romania has a complex stratigraphy and the archaeologists have carried out analyses on Chalcolithic (Precucuteni and Cucuteni and Bronze Age levels.

  11. Características morfológicas da distribuição vascular cerebral de Sus scrofa Linnaeus (Mammalia, Artiodactyla Morphological characteristics of the cerebral vascular distribution of Sus scrofa Linnaeus (Mammalia, Artiodactyla

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    Jurandyr de A. Câmara Filho

    2004-12-01

    Full Text Available O objetivo do presente estudo foi verificar a vascularização arterial do encéfalo do javali, Sus scrofa Linnaeus, 1758, Suidae, e comparar com outras espécies. Cinco machos e cinco fêmeas foram injetados com látex colorido. O suprimento arterial do encéfalo foi descrito e análises morfológicas foram feitas. Nesta espécie o circuito arterial do encéfalo é formado por ramos da artéria carótida interna, como: as artérias comunicantes caudais, o ramo rostral, as artérias cerebrais rostrais, e artérias comunicantes rostrais.The aim this study was to verify the arterial vascularization of the wild boar brain, Sus scrofa Linnaeus, 1758, Suidae, and compare with other species. Five male and five female were injected with colored latex. The arterial supply of the brain was described and were done morphological analyses. In this species the arterial circuit of the brain is formed by the internal carotid artery branches, such as: the caudal communicant arteries, rostral branch, rostral cerebral arteries and rostral communicant arteries.

  12. THE CHALLENGE OF DETECTING CLASSICAL SWINE FEVER VIRUS CIRCULATION IN WILD BOAR (SUS SCROFA): SIMULATION OF SAMPLING OPTIONS.

    Science.gov (United States)

    Sonnenburg, Jana; Schulz, Katja; Blome, Sandra; Staubach, Christoph

    2016-10-01

    Classical swine fever (CSF) is one of the most important viral diseases of domestic pigs ( Sus scrofa domesticus) and wild boar ( Sus scrofa ). For at least 4 decades, several European Union member states were confronted with outbreaks among wild boar and, as it had been shown that infected wild boar populations can be a major cause of primary outbreaks in domestic pigs, strict control measures for both species were implemented. To guarantee early detection and to demonstrate freedom from disease, intensive surveillance is carried out based on a hunting bag sample. In this context, virologic investigations play a major role in the early detection of new introductions and in regions immunized with a conventional vaccine. The required financial resources and personnel for reliable testing are often large, and sufficient sample sizes to detect low virus prevalences are difficult to obtain. We conducted a simulation to model the possible impact of changes in sample size and sampling intervals on the probability of CSF virus detection based on a study area of 65 German hunting grounds. A 5-yr period with 4,652 virologic investigations was considered. Results suggest that low prevalences could not be detected with a justifiable effort. The simulation of increased sample sizes per sampling interval showed only a slightly better performance but would be unrealistic in practice, especially outside the main hunting season. Further studies on other approaches such as targeted or risk-based sampling for virus detection in connection with (marker) antibody surveillance are needed.

  13. Heavy metals in wild boar (Sus scrofa and related lesions

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    A. Zaccaroni

    2003-10-01

    Full Text Available Heavy metals are toxic elements naturally present in the environment which can be bioconcentrated by plants and animals and incorporated into food cycles. Thus the use of wildlife species as monitors is a useful tool to assess environmental pollution. Because of its ethological characteristics, wild boar is highly probable to be exposed to pollution, mainly by heavy metals. These pollutants, when present at high enough concentrations, can induce histological lesion in various tissues. The purpose of the present work was to investigate the levels of five metals (cadmium, copper, iron, lead and zinc in wild boar, Sus scrofa, originating from an Apennine area of Emilia Romagna, Northern Italy, and to evaluate any possible correlation with histological lesions eventually observed. Samples of several organs (liver, heart, kidney, diaphragm, abductor muscle, masseter, eye, testis, brain, lung and spleen were collected from hunted animals during winter 2001. Levels of metals were determined on freeze dried tissues employing microwave wet digestion. Toxicological analysis were performed by atomic absorption spectrophotometry and data expressed on a dry weight basis. Histopatological analyses were performed on fixed samples of the same tissue. Highest mean values were found in kidney for cadmium and copper (7 ± 1 ppm and 30 ± 2.6 ppm respectively and in liver for copper (21.16 ± 3.28 ppm, lead (2139 ± 680.6 ppb, iron (415.92 ± 24.41 ppm and zinc (94.76 ± 6.11 ppm. In all other tissues, mean concentrations of each metal were at low levels, and were comparable one another. A statistical difference was found for iron (p<0.05, copper (p<0.05 and cadmium (p<0.01 in kidney, in testis (p<0.01 for zinc and in liver for copper (p<0.05 and cadmium (p<0.01 as function of age cohorts. When sex was considered, a statistical difference was found for iron in eye, for copper in heart and for cadmium in heart and kidney. Histopatological analysis allowed

  14. Molecular survey of porcine teschovirus, porcine sapelovirus, and enterovirus G in captive wild boars (Sus scrofa scrofa of Paraná state, Brazil

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    Daiane G. Donin

    2015-05-01

    Full Text Available AbstractPorcine teschovirus (PTV, porcine sapelovirus (PSV, and enterovirus G (EV-G are infectious agents specific to pig host species that are endemically spread worldwide. This study aimed to investigate the natural infection by these porcine enteric picornaviruses in wild boars (Sus scrofa scrofa of Paraná state, Brazil, and to evaluate peccaries (Pecari tajacu and Tayassu pecari as alternative host species for these viruses. Fecal samples (n=36 from asymptomatic wild boars (n=22 with ages ranging from 2 to 7 months old (young, n=14 and 2 to 4 years old (adult, n=8 and from peccaries (6 to 8 months old, n=14 were collected from a farm and a zoo, respectively, both located in Paraná state. Reverse transcription-polymerase chain reaction (RT-PCR and nested-PCR (n-PCR assays targeting the 5'non-translated region of the virus genome were used for screening the viruses. Porcine enteric picornaviruses were detected in 12 out of the 22 wild boar fecal samples. According to each of the viruses, EV-G was most frequently (11/22, 50% detected, followed by PTV (10/22, 45.5% and PSV (4/22, 18.2%. Regarding the age groups, young wild boars were more frequently (9/14, 64.3% infected with PTV, PSV, and EV-G than adult animals (3/8, 37.4%. One n-PCR amplified product for each of the viruses was submitted to sequencing analysis and the nucleotide sequences were compared with the related viruses, which showed similarities varying from 97.7% to 100% for PTV, 92.4% to 96.2% for PSV, and 87.1% to 100% for EV-G. Peccaries tested negative for the viruses and in this study they did not represent infection reservoirs. This study is the first to report the molecular detection of PTV, PSV, and EV-G from captive wild boars in a South American country and the first to screen peccaries as alternative host species for porcine enteric picornavirus.

  15. Substitution within erythropoietin receptor gene D1 domain associated with litter size in Beijing Black pig, Sus scrofa.

    Science.gov (United States)

    Zhang, Longchao; Wang, Ligang; Li, Yong; Li, Wen; Yan, Hua; Liu, Xin; Zhao, Kebin; Wang, Lixian

    2011-10-01

    Studies of uterine capacity and litter size in swine have suggested that erythropoietin receptor (EPOR) plays an important role in fetal survival through maturation of red blood cells. In this study, we screened the porcine EPOR gene for mutations and identified three single nucleotide polymorphisms (SNPs): two missense mutations and one synonymous mutation. We then genotyped 272 Beijing Black sows, Sus scrofa, and compared this data with litter sizes from a total of 1523 parities among the sows. The G allele of the nonsynonymous SNP, EPOR c.434A>G, was associated with greater litter size at both first parity (P G could be a useful genetic marker to improve litter size in swine.

  16. Spargana in a weasel, Mustela sibirica manchurica, and a wild boar, Sus scrofa, from Gangwon-do, Korea.

    Science.gov (United States)

    Lee, Seung-Ha; Choe, Eun-Yoon; Shin, Hyun-Duk; Seo, Min

    2013-06-01

    To know the status of sparganum (plerocercoid of Spirometra erinacei) infection in the Korean wild life, several species of wild animals were captured in Gangwon-do and examined for their status of infection with spargana. From February to December 2011, a total of 62 wild boars, 5 badgers, 1 weasel, 1 Siberian chipmunk, and 53 wild rodents were captured, and their whole muscles were examined with naked eyes for the presence of spargana worms. From the weasel and 1 wild boar, a total of 5 spargana specimens were extracted. The weasel was for the first time recorded as an intermediate or paratenic/transport host of S. erinacei in Korea, and both the weasel (Mustela sibirica manchurica) and wild boar (Sus scrofa) were added to the list of wild animals carrying spargana.

  17. Seroprevalence of Trichinella sp. in Wild Boars (Sus scrofa) from Yanggu-gun, Gangwon-do, Korea.

    Science.gov (United States)

    Lee, Hye-Jung; Chung, Ok-Sik; Kim, Jae-Lip; Lee, Seung-Ha; Yoo, Young-Bok; Seo, Min

    2015-04-01

    A total 7 outbreaks of trichinellosis have occurred in Korea, mostly as a result of consumption of raw wild boar (Sus scrofa) meat. Since only 1 serological survey on wild boars had yet been performed in Korea, the present study aimed to estimate the prevalence of trichinellosis in wild boars and some species of rodents by artificial digestion and serological examinations in Yanggu-gun, Gangwon-do, the endemic area of trichinellosis. Both the wild boar and rodent muscle samples revealed no Trichinella larvae by direct examination and artificial digestion method. However, serological examinations revealed that 4 wild boar sera samples out of 118 (3.4%) were positive to Trichinella antigen. Although the recovery of Trichinella larvae ended in a failure, it is proved for the first time that the sylvatic cycle of Trichinella has been maintained in wild boars of Gangwon-do, Korea.

  18. Volatile emission of decomposing pig carcasses (Sus scrofa domesticus L.) as an indicator for the postmortem interval.

    Science.gov (United States)

    Paczkowski, Sebastian; Nicke, Sara; Ziegenhagen, Henrik; Schütz, Stefan

    2015-01-01

    This study aimed at correlating selected carcass borne volatile organic compounds (VOCs) with the postmortem interval (PMI). Selected volatiles should 1st be reliably emitted during vertebrate decay, 2nd be emitted at high concentrations, and 3rd show a reproducible quantitative dynamic during the decaying process. Four pigs (Sus scrofa domesticus L.) were placed in a deciduous forest in different seasons and volatiles emitted during the decaying process were sampled. Seventeen compounds were identified and quantified by GC-MS. Electrophysiological experiments on the antenna of female Calliphora vicina and additional data of Dermestes maculans were used as an evolutionary tuned information filter to evaluate the 1st criterion. The relative quantitative emission of hexanal, nonanal, dimethyl disulfide, dimethyl trisulfide, 1-butanol, and phenol were correlated with the PMI, and the observed stages of decay and the limitations of this model were discussed.

  19. Multiple Origins and Admixture of Recently Expanding Japanese Wild Boar (Sus scrofa leucomystax) Populations in Toyama Prefecture of Japan.

    Science.gov (United States)

    Yamazaki, Yuji; Adachi, Fuminari; Sawamura, Akira

    2016-02-01

    Japanese wild boar (Sus scrofa leucomystax) populations have expanded drastically throughout the Japanese Archipelago in recent decades. To elucidate the dispersal patterns of Japanese wild boar in Toyama Prefecture in central Japan, we used a multi-locus microsatellite DNA analysis to determine its population structure and the degree of admixture. The deviation from Hardy-Weinberg equilibrium was detected in either total or separate regional wild boar samples from Toyama Prefecture. This result could be explained by the Wahlund effect resulting from the mixture of samples from different sources. Bayesian structure analysis, assignment test, and factorial correspondence analysis suggested that wild boars around Toyama Prefecture derive from at least two ancestral sources. The migration and possible mating of each individual may have occurred recently and continued in each geographically neighboring region. The present genetic results may be useful for prediction of future dispersal patterns of Japanese wild boar, as well as other animals in expansion.

  20. δ18O values of Sus scrofa blood water and bone phosphate; a marked discrepancy between domestic and wild specimens.

    Science.gov (United States)

    Longinelli, Antonio; Selmo, Enrico

    2011-12-30

    δ¹⁸O analyses of water in the blood of domestic and wild pigs indicated that large isotopic differences exist between domestic and wild specimens of the same species (Sus scrofa) living in the same area. Similar isotopic differences are found between the δ¹⁸O(PO₄³⁻) values of bones from the two groups of animals. When δ¹⁸O values obtained from recent wild boar bones are introduced in the equation of the isotopic scale determined for domestic pigs, totally unreliable δ¹⁸O values of local meteoric water are obtained. The δ¹⁸O(PO₄³⁻) values measured in three groups of modern wild boar specimens allow the calculation of a first approximate equation which is quite different from that of domestic pigs. This isotopic scale should be accurately re-calibrated for wild animals.

  1. Observation of Intestinal Nematodes and Protozoan Parasite in Sus Scrofa%秦岭北坡野猪肠道寄生虫感染情况调查

    Institute of Scientific and Technical Information of China (English)

    胡罕; 车利锋; 张洪峰; 刘艳; 乔继英; 吴晓民

    2013-01-01

      Focus on understanding and diagnosis intestinal parasitic infection in living Sus scrofa for the further treatment ,feces from Sus scrofa grown in northern slope of the Qinling mountain were examined in september 2012 .Wet mount smear and iodine staining were employed to check fecal samples from 5 Sus scrofa .Seven specices of parasites were detected ,including Balantidium coli cyst ,Eimeria ,Ascarts egg ,Globo-cephalus egg ,Metastrongylus apri egg ,Oesophagostomum dentatum egg and Stephanu- rusdentatus egg ,as the reference for disease prevention .%  为了了解秦岭北坡野猪肠道寄生虫感染情况,课题组于2012年9月在秦岭北坡采集5只野猪粪便并进行检查。通过生理盐水涂片、碘液染色法检出结肠小袋纤毛虫包囊、猪艾美耳球虫、蛔虫卵、球首线虫卵、食道口线虫卵、猪肾虫卵等寄生虫及虫卵,为野猪疾病的及早发现和确认提供依据,为野猪寄生虫病的防治提供参考。

  2. Prevalence and diversity of Encephalitozoon spp. and Enterocytozoon bieneusi in wild boars (Sus scrofa) in Central Europe.

    Science.gov (United States)

    Němejc, Karel; Sak, Bohumil; Květoňová, Dana; Hanzal, Vladimír; Janiszewski, Paweł; Forejtek, Pavel; Rajský, Dušan; Kotková, Michaela; Ravaszová, Petra; McEvoy, John; Kváč, Martin

    2014-02-01

    From 2011 to 2012, the occurrence of Enterocytozoon bieneusi and Encephalitozoon spp. was surveyed at 29 randomly selected localities (both forest areas and enclosures) across four Central European countries: Austria, the Czech Republic, Poland, and the Slovak Republic. Isolates were genotyped by PCR amplification and characterization of the internal transcribed spacer (ITS) region using Enterocytozoon and Encephalitozoon-specific protocols. PCR revealed 16 mono-infections of Encephalitozoon cuniculi, 33 mono-infections of Enterocytozoon bieneusi and 5 concurrent infections of both Encephalitozoon cuniculi and Enterocytozoon bieneusi out of 460 faecal samples. Two genotypes (I and II) were revealed by sequence analysis of the ITS region of Encephalitozoon cuniculi. Eleven genotypes, five previously found in other hosts including domestic pigs (D, EbpA, EbpC, G and Henan-I) and six novel (WildBoar1-6), were identified in Enterocytozoon bieneusi. No other microsporidia infection was found in the examined faecal samples. Prevalence of microsporidia at the locality level ranged from 0 to 58.8 %; the prevalence was less than 25 % at more than 86 % of localities. Enterocytozoon bieneusi was detected as a predominant species infecting Eurasian wild boars (Sus scrofa). The present report is the most comprehensive survey of microsporidia infections in wild boars within the Czech Republic and selected Central European countries.

  3. Arterial supply of the cervical thymus lobes in swine fetuses (Sus scrofa domesticus of the Camborough 25 lineage

    Directory of Open Access Journals (Sweden)

    Eduardo Maurício Mendes de Lima

    2009-09-01

    Full Text Available This study aims to discover more about the arterial supply of cervical lobes of the thymus, in relation to its origins, distribution and ordination in swine (Sus scrofa domesticus of Camborough 25 lineage. Thus, 29 swine (17 males and 12 females were studied. The arterial system of the models was colored with aqueous solution of Neoprene Latex “450” (50% and after the models were fixed in 10% formaldehyde aqueous solution. Great individual variations in the length and size of the thymus vessel supply, and also in the layout, size and thickness of the cervical lobes of the thymus, were observed. The superficial cervical artery (96.55%, common carotid artery (54.54% and occipital artery (55.17% contribute to the blood supply of the cervical lobes on both sides. In addition, branches from the lingual artery (3.45% and internal carotid artery (3.45% on both sides, as well as the subclavian artery (6.90% on the left side, were observed. No significant differences in vascularization of lobes in relation to antimerals or sexes were evidenced. Considering the findings, it was conclued that each animal had its own characteristics and peculiarities, thus enabling the establishment of a particular arrangement of the swine strain.

  4. Genetic variation of the East Balkan Swine (Sus scrofa) in Bulgaria, revealed by mitochondrial DNA and Y chromosomal DNA.

    Science.gov (United States)

    Hirata, D; Doichev, V D; Raichev, E G; Palova, N A; Nakev, J L; Yordanov, Y M; Kaneko, Y; Masuda, R

    2015-04-01

    East Balkan Swine (EBS) Sus scrofa is the only aboriginal domesticated pig breed in Bulgaria and is distributed on the western coast of the Black Sea in Bulgaria. To reveal the breed's genetic characteristics, we analysed mitochondrial DNA (mtDNA) and Y chromosomal DNA sequences of EBS in Bulgaria. Nucleotide diversity (πn ) of the mtDNA control region, including two newly found haplotypes, in 54 EBS was higher (0.014 ± 0.007) compared with that of European (0.005 ± 0.003) and Asian (0.006 ± 0.003) domestic pigs and wild boar. The median-joining network based on the mtDNA control region showed that the EBS and wild boar in Bulgaria comprised mainly two major mtDNA clades, European clade E1 (61.3%) and Asian clade A (38.7%). The coexistence of two mtDNA clades in EBS in Bulgaria may be the relict of historical pig translocation. Among the Bulgarian EBS colonies, the geographical differences in distribution of two mtDNA clades (E1 and A) could be attributed to the source pig populations and/or historical crossbreeding with imported pigs. In addition, analysis of the Y chromosomal DNA sequences for the EBS revealed that all of the EBS had haplotype HY1, which is dominant in European domestic pigs.

  5. A Taphonomic Study Exploring the Differences in Decomposition Rate and Manner between Frozen and Never Frozen Domestic Pigs (Sus scrofa).

    Science.gov (United States)

    Roberts, Lindsey G; Dabbs, Gretchen R

    2015-05-01

    This research examined differences in decomposition rate and manner of domestic pig subjects (Sus scrofa) in never frozen (control) and previously frozen (experimental) research conditions. Eight control and experimental subjects were placed in an identical outdoor research environment. Daily quantitative and qualitative measurements were collected: abdominal circumference, total body score (TBS), temperature, photographs, descriptive decomposition stages, and visual observations. Field necropsies were performed at accumulated degree days (ADD) between 50 and 300 (Celsius). Paired samples t-tests of ADD to TBS >3.0, TBS >9.5, and TBS >16.0 indicate the rate of decomposition of experimental subjects was significantly slower than controls at both TBS >3 and >9.5 (p = 0.003 and p = 0.002, respectively). A suite of qualitative indicators of predecomposition freezing is also reported. The differences between experimental and control subjects suggest previously frozen subjects should not be used in taphonomic research, as results do not accurately reflect the "normal" taphonomic condition.

  6. Evaluation of a Commercial ELISA for the Detection of Antibodies to Sarcoptes scabiei in Wild Boar (Sus scrofa).

    Science.gov (United States)

    Haas, Chloé; Rossi, Sophie; Meier, Roman; Ryser-Degiorgis, Marie-Pierre

    2015-07-01

    Sarcoptic mange occurs in free-ranging wild boar (Sus scrofa) but has been poorly described in this species. We evaluated the performance of a commercial indirect enzyme-linked immunosorbent assay (ELISA) for serodiagnosis of sarcoptic mange in domestic swine when applied to wild boar sera. We tested 96 sera from wild boar in populations without mange history ("truly noninfected") collected in Switzerland between December 2012 and February 2014, and 141 sera from free-ranging wild boar presenting mange-like lesions, including 50 live animals captured and sampled multiple times in France between May and August 2006 and three cases submitted to necropsy in Switzerland between April 2010 and February 2014. Mite infestation was confirmed by skin scraping in 20 of them ("truly infected"). We defined sensitivity of the test as the proportion of truly infected that were found ELISA-positive, and specificity as the proportion of truly noninfected that were found negative. Sensitivity and specificity were 75% and 80%, respectively. Success of antibody detection increased with the chronicity of lesions, and seroconversion was documented in 19 of 27 wild boar sampled multiple times that were initially negative or doubtful. In conclusion, the evaluated ELISA has been successfully applied to wild boar sera. It appears to be unreliable for early detection in individual animals but may represent a useful tool for population surveys.

  7. Associations of MYF5 gene polymorphisms with meat quality traits in different domestic pig (Sus scrofa populations

    Directory of Open Access Journals (Sweden)

    Min Liu

    2007-03-01

    Full Text Available The MYF5 gene is first inducibly expressed in muscle cell during embryonic muscle development and plays an important role in regulating the differentiation of skeletal muscle precursors. In this study we used PCR-RFLP to investigate two pig (Sus scrofa populations (n = 302 for two MYF5 gene polymorphisms, a previously unreported novel Met-Leu shift single nucleotide polymorphism (SNP MYF5/Hsp92II located on exon 1 and the previously identified intron 1 MYF5/HinfI SNP. Haplotype and association analysis showed that haplotypes of the two SNPs were significantly associated with drip loss rate (DLR, p < 0.05, water holding capacity (WHC, p < 0.05, biceps femoris meat color value (MCV2, p < 0.05, biceps femoris marbling score (MM2, p < 0.01, longissimus dorsi intramuscular fat percentage (IMF, p < 0.01 and longissimus dorsi Water moisture content (WM, p < 0.01 in the population 2. However, further studies are needed to confirm these preliminary results.

  8. Enhancing the antimicrobial activity of Sus scrofa lysozyme by N-terminal fusion of a sextuple unique homologous peptide.

    Science.gov (United States)

    Zhu, Dewei; Cai, Guolin; Li, Xiaomin; Lu, Jian; Zhang, Liang

    2017-02-10

    Sus scrofa lysozyme (SSL), an important component of the pig immune system, is a potential candidate to replace antibiotics in feed. However, there is little antimicrobial activity of natural SSL against gram-negative bacteria, which limits its application. In this study, a unique peptide (A-W-V-A-W-K) with antimicrobial activity against gram-negative bacteria was discovered and purified from trypsin hydrolysate of natural SSL. This unique peptide was fused to natural SSL and the recombinant fused SSL exhibited improved activity against gram-negative bacteria. The N-terminal fusion likely increased the membrane penetrability and induced programmed bacterial cell death. The recombinant fused SSL also showed higher activity against some gram-positive bacteria with O-acetylation. By N-terminal fusion of the sextuple peptide, the anti-microbial activity, either to gram-positive or negative bacteria, of the recombinant SSL was higher than the fusion of only one copy of the peptide. This study provides a general, feasible, and highly useful strategy to enhance the antimicrobial activity of lysozyme.

  9. Evidence of leptospirosis in the kidneys and serum of feral swine (Sus scrofa) in the United States.

    Science.gov (United States)

    Pedersen, K; Anderson, T D; Bevins, S N; Pabilonia, K L; Whitley, P N; Virchow, D R; Gidlewski, T

    2017-01-01

    Leptospirosis is the most widespread zoonosis in humans worldwide. In the United States, widespread detection of antibodies to leptospirosis have been identified in feral swine (Sus scrofa) with the highest detection of serovars, Bratislava, Icterohaemorrhagiae, and Pomona. Over the past few years, feral swine populations have expanded their geographical range and distribution in the United States with reports in at least 39 of 50 states. Since feral swine serve as reservoirs for serovars that can infect humans, it is important to understand the risk of transmission. In order to learn more about the probability that feral swine shed infectious leptospires, we collected kidneys and paired serum when possible from 677 feral swine in 124 counties of 29 states. These counties had previously been identified as antibody positive for Leptospira interrogans serovars Bratislava, Canicola, Grippotyphosa, Hardjo, Icterohaemorrhagiae or Pomona. Although exposure to these same six serovars of leptospirosis continued to be high (53% overall) in the counties we sampled, we detected leptospiral DNA in only 3·4% of feral swine kidneys tested. Based on these results, it appears that although feral swine can serve as a source of infection to humans, especially in those who are more likely to encounter them directly such as wildlife biologists, veterinarians, and hunters, the risk may be relatively low. However, further studies to examine the relationship between leptospiral shedding in the urine and kidneys in addition to culturing the organism are recommended in order to better understand the risk associated with feral swine.

  10. CHARACTERIZATION OF PORCINE PARVOVIRUS TYPE 3 AND PORCINE CIRCOVIRUS TYPE 2 IN WILD BOARS (SUS SCROFA) IN SLOVAKIA.

    Science.gov (United States)

    Sliz, Ivan; Vlasakova, Michaela; Jackova, Anna; Vilcek, Stefan

    2015-07-01

    As the number of free-living wild boars (Sus scrofa L.) continues to rise in Slovakia, the probability of pathogen transmission between susceptible species increases. We investigated the distribution and genetic characterization of porcine parvovirus type 3 (PPV3), porcine circovirus type 2 (PCV2), and their coinfection in wild boars. Among 194 animals tested, 19.1% were positive for PPV3 and 43.8% for PCV2. Similar rates of coinfection with both viruses reaching 11.0% and 11.8% were observed in juvenile and mature wild boars, respectively. Phylogenetic analysis of PPV3 sequences from VP1 and NS1 genomic regions revealed a close genetic relationship among isolates from Slovakia and those sampled worldwide. Prevalence of PCV2 in wild boars was lower than that reported in domestic pigs in Slovakia. The PCV2 variants originating from sylvatic and domestic hosts in Slovakia were grouped in the same clusters, namely PCV2b-1A/1B and PCV2a-2D.

  11. Non-tuberculous mycobacteria in wild boar (Sus scrofa) from Southern Spain: epidemiological, clinical and diagnostic concerns.

    Science.gov (United States)

    García-Jiménez, W L; Benítez-Medina, J M; Martínez, R; Carranza, J; Cerrato, R; García-Sánchez, A; Risco, D; Moreno, J C; Sequeda, M; Gómez, L; Fernández-Llario, P; Hermoso-de-Mendoza, J

    2015-02-01

    Non-tuberculous mycobacteria (NTM) are widely distributed in the environment, particularly in wet soil, marshland, rivers or streams, but also are causative agents of a wide variety of infections in animals and humans. Little information is available regarding the NTM prevalence in wildlife and their effects or significance in the bovine tuberculosis (bTB) epidemiology and diagnosis. This research shows the most frequently NTM isolated in lymph nodes of wild boar (Sus scrofa) from southern Spain, relating the NTM presence with the individual characteristics, the management of animals and the possible misdiagnosis of Mycobacterium bovis in concurrent infections. A total of 219 NTM isolates were obtained from 1249 wild boar mandibular lymph nodes sampled between 2007 and 2011. All but 75 isolates were identified by the PCR-restriction analysis-hsp65, and a partial sequencing of the 16S rDNA was carried out to identify the rest of the isolates. Results showed that Mycobacterium chelonae was the most frequently isolated NTM specie (133 isolates, 60.7%), followed by Mycobacterium avium (24 isolates, 11%). No relation was found regarding sex, body condition and management, but M. chelonae was more frequently detected in adults, whereas M. avium was more prevalent in subadults. The high NTM prevalence observed in the studied wild boar populations could make difficult the bTB diagnostic.

  12. Onchocerca takaokai n. sp. (Nematoda: Filarioidea) in Japanese wild boars (Sus scrofa leucomystax): Description and molecular identification of intradermal females.

    Science.gov (United States)

    Uni, Shigehiko; Fukuda, Masako; Agatsuma, Takeshi; Bain, Odile; Otsuka, Yasushi; Nakatani, Jun; Matsubayashi, Makoto; Harada, Masashi; Omar, Hasmahzaiti; Ramli, Rosli; Hashim, Rosli; Azirun, Mohd Sofian; Takaoka, Hiroyuki

    2015-12-01

    Human zoonotic onchocercosis is caused by Onchocerca dewittei japonica, parasitic in wild boars (Sus scrofa leucomystax) in Japan. Previously, microfilariae longer than those of Onchocerca dewittei japonica were observed in skin snips from wild boars during the study of O. dewittei japonica. Moreover, the third-stage larvae (L3) of these longer microfilariae were obtained from the blackfly Simulium bidentatum after experimental injections. Based on morphometric and molecular studies, similar L3 were found in blackflies during fieldwork in Oita, Japan. However, except for O. dewittei japonica, adult worms of Onchocerca have not been found in wild boars. In this study, we discovered adult females of a novel Onchocerca species in the skin of a wild boar in Oita, and named it Onchocerca takaokai n. sp. Females of this new species had longer microfilariae and differed from O. dewittei japonica in terms of their morphological characteristics and parasitic location. The molecular characteristics of the cytochrome c oxidase subunit 1 and 12S rRNA genes of the new species were identical to those of the longer microfilariae and L3 previously detected, but they differed from those of O. dewittei japonica at the species level. However, both species indicated a close affinity among their congeners and Onchocerca ramachandrini, parasitic in the warthog in Africa, was basal in the Suidae cluster of the 12S rRNA tree.

  13. Mesenteric lymph node granulomatous lesions in naturally infected wild boar (Sus scrofa) in Portugal--Histological, immunohistochemical and molecular aspects.

    Science.gov (United States)

    Matos, A C; Andrade, S; Figueira, L; Matos, M; Pires, M A; Coelho, A C; Pinto, M L

    2016-05-01

    Several studies have demonstrated that wildlife reservoirs of mycobacteria are responsible for the maintenance and spreading of the infection to livestock and wildlife counterparts. Recent data report the role of wild boar (Sus scrofa) as a reservoir for Mycobacterium bovis. This study was conducted to evaluate the chronic inflammatory response in the mesenteric lymph nodes (MLN) of wild boar with granulomatous lymphadenitis (n=30). Morphological parameters of the lesions were recorded. The expression of CD3 and CD79α molecules was evaluated by immunohistochemistry. Molecular genotyping and culture to identify mycobacteria were performed. The lesions consisted mainly of stage III and stage IV granulomas. CD3 and CD79α positive cells were observed in 15 (50%) and in 11 (36.6%) MLN, respectively. In these lesions, higher percentages of T lymphocytes were found and a limited number of animals exhibited a tendency for an increased percentage of B lymphocytes. Our results suggest that there are similar percentages and distribution patterns of CD3 and CD79α in the lesions, regardless of the presence of Mycobacterium avium subsp. paratuberculosis (Map), M. bovis or Map-M. bovis co-infection, and confirm that wild boar is both susceptible and could be an important Map and M. bovis wild reservoir in the study area.

  14. Characterization of glutathione S-transferases from Sus scrofa, Cydia pomonella and Triticum aestivum: their responses to cantharidin.

    Science.gov (United States)

    Yang, Xue-Qing; Zhang, Ya-Lin

    2015-02-01

    Glutathione S-transferases (GSTs) play a key role in detoxification of xenobiotics in organisms. However, their other functions, especially response to the natural toxin cantharidin produced by beetles in the Meloidae and Oedemeridae families, are less known. We obtained GST cDNAs from three sources: Cydia pomonella (CpGSTd1), Sus scrofa (SsGSTα1), and Triticum aestivum (TaGSTf3). The predicted molecular mass is 24.19, 25.28 and 24.49 kDa, respectively. These proteins contain typical N-terminal and C-terminal domains. Recombinant GSTs were heterologously expressed in Escherichia coli as soluble fusion proteins. Their optimal activities are exhibited at pH 7.0-7.5 at 30 °C. Activity of CpGSTd1 is strongly inhibited by cantharidin and cantharidic acid, but is only slightly suppressed by the demethylated analog of cantharidin and cantharidic acid. Enzymatic assays revealed that cantharidin has no effect on SsGSTα1 activity, while it significantly stimulates TaGSTf3 activity, with an EC50 value of 0.3852 mM. Activities of these proteins are potently inhibited by the known GST competitive inhibitor: S-hexylglutathione (GTX). Our results suggest that these GSTs from different sources share similar structural and biochemical characteristics. Our results also suggest that CpGSTd1 might act as a binding protein with cantharidin and its analogs.

  15. Survey of Feral Swine ( Sus scrofa ) Infection with the Agent of Chagas Disease ( Trypanosoma cruzi ) in Texas, 2013-14.

    Science.gov (United States)

    Comeaux, Juliette M; Curtis-Robles, Rachel; Lewis, Barbara C; Cummings, Kevin J; Mesenbrink, Brian T; Leland, Bruce R; Bodenchuk, Michael J; Hamer, Sarah A

    2016-07-01

    : Feral swine ( Sus scrofa ) are an invasive species and reservoir of numerous zoonotic pathogens in the US, and Texas leads the nation in the estimated population size of feral hogs. Texas also harbors enzootic transmission cycles of the protozoan parasite Trypanosoma cruzi , agent of Chagas disease. Given previous evidence that swine can serve as reservoirs of T. cruzi in Latin America and new evidence of triatomines (kissing bugs) feeding on swine in Texas, we measured the prevalence of T. cruzi infection in feral swine in Texas. From 2013 to 2014, we sampled blood and/or cardiac tissue from 78 feral swine across 14 Texas counties (seven with and seven without prior documentation of kissing bug occurrence) and used PCR and histopathology to detect T. cruzi infection. We determined an overall infection prevalence of 6% (3 of 54) based on PCR evaluation of cardiac tissue, and no blood samples were positive (n=72). All three positive pigs were from counties where kissing bugs are documented. No T. cruzi amastigotes were noted on histopathology (n=54). Sarcocysts were observed in 10 (18%) of the samples, five of which also had mild focal areas of degeneration and inflammatory cell infiltration. Eco-epidemiologic investigations can provide an assessment of contributions of feral hogs to maintenance of T. cruzi across a landscape to help protect human and animal health.

  16. Relative age of proviral porcine endogenous retrovirus sequences in Sus scrofa based on the molecular clock hypothesis.

    Science.gov (United States)

    Tönjes, Ralf R; Niebert, Marcus

    2003-11-01

    Porcine endogenous retroviruses (PERV) are discussed as putative infectious agents in xenotransplantation. PERV classes A, B, and C harbor different envelope proteins. Two different types of long terminal repeat (LTR) structures exist, of which both are present only in PERV-A. One type of LTR contains a distinct repeat structure in U3, while the other is repeatless, conferring a lower level of transcriptional activity. Since the different LTR structures are distributed unequally among the proviruses and, apparently, PERV is the only virus harboring two different LTR structures, we were interested in determining which LTR is the ancestor. Replication-competent viruses can still be found today, suggesting an evolutionary recent origin. Our studies revealed that the age of PERV is at most 7.6 x 10(6) years, whereas the repeatless LTR type evolved approximately 3.4 x 10(6) years ago, being the phylogenetically younger structure. The age determined for PERV correlates with the time of separation between pigs (Suidae, Sus scrofa) and their closest relatives, American-born peccaries (Tayassuidae, Pecari tajacu), 7.4 x 10(6) years ago.

  17. Vegetable and animal food sorts found in the gastric content of Sardinian Wild Boar (Sus scrofa meridionalis).

    Science.gov (United States)

    Pinna, W; Nieddu, G; Moniello, G; Cappai, M G

    2007-06-01

    Authors report results emerging from gastric content analysis from n. 96 wild boars hunted in Sardinia isle, during the hunting tide (2001-2005), from November to January. Mean pH of the gastric content was 3.77 +/- 0.69. Mean total capacity (TC) of each stomach was 1702 +/- 680 g. Mean Stuff ratio (CW/TC) between the content weight (CW) and stomachs TC was 0.45. Food categories found in animal stomachs were: 19 categories of vegetal species (Allium spp., Arbutus unedo, Arisarum vulgare, Avena fatua, Avena sativa, Castanea sativa, Ceratonia siliqua, Chamaerops umilis, Cichorium intybus, Hordeum sativum, Juniperus oxycedrus, Myrtus communis, Olea europea, Pirus amygdaliformis, Pistacia lentiscus, Quercus spp., Rhamnus alaternus, Triticum durum, Zea mais); 11 categories of animal species (Agriotes lineatus, Apodemus sylvaticus dicrurus, Chalcides chalcides, Chalcides ocellatus tiligugu, Crematogaster scutellaris, Forficula auricularia, Helix aspersa, Lumbricus terrestris, Ovis aries, Podarcis tiliguerta tiliguerta, Scolopendra cingulata); three categories were identified in general terms (insects larvae, hairs of mammals, feathers of birds). Food categories found in the stomach contents of Sus scrofa meridionalis confirm observations by other researchers who report the prevalence of vegetables in spite of animal food sorts in the wild boar diet in Italian regions.

  18. Spatial distribution of Trichinella britovi, T. spiralis and T. pseudospiralis of domestic pigs and wild boars (Sus scrofa) in Hungary.

    Science.gov (United States)

    Széll, Z; Marucci, G; Ludovisi, A; Gómez-Morales, M A; Sréter, T; Pozio, E

    2012-02-10

    Trichinellosis is a foodborne disease caused by the consumption of raw meat and raw meat-derived products from swine, horse and some game animals infected with nematode worms of the genus Trichinella. Between June 2006 and February 2011, 16 million domestic pigs and 0.22 million wild boars (Sus scrofa) were tested for Trichinella sp. in Hungary. Trichinella infection was not found in any pigs slaughtered for public consumption. Nevertheless, Trichinella spiralis was detected in four backyard pigs when trace back was done following a family outbreak. Trichinella infection was demonstrated in 17 wild boars (0.0077%). Larvae from wild boars were identified as Trichinella britovi (64.7%), T. spiralis (29.4%) and Trichinella pseudospiralis (5.9%). Although the prevalence of Trichinella sp. infection in wild boars and domestic pigs is very low, the spatial analysis reveals that the level of risk differs by region in Hungary. Most of the T. britovi infected wild boars (63.6%) were shot in the north-eastern mountain area of Hungary; whereas domestic pigs and wild boars infected with T. spiralis were detected only in the southern counties bordering Croatia and Romania. In the north-western and central counties, the prevalence of Trichinella infection seems to be negligible.

  19. The first report on Cryptosporidium suis and Cryptosporidium pig genotype II in Eurasian wild boars (Sus scrofa) (Czech Republic).

    Science.gov (United States)

    Němejc, Karel; Sak, Bohumil; Květoňová, Dana; Hanzal, Vladimír; Jeníková, Martina; Kváč, Martin

    2012-03-23

    A total of 193 faecal samples of adult Eurasian wild boars were collected at 12 enclosures across the Czech Republic and examined for Cryptosporidium infection using both microscopic and molecular tools. Cryptosporidium oocysts were not detected in any of the 193 faecal samples examined using the aniline-carbol-methyl violet staining method. Thirty-two positive cases of Cryptosporidium infection were detected using either genus- or species-specific nested PCR. Mono-infection with Cryptosporidium suis and Cryptosporidium pig genotype II were found in 13 and 7 cases, respectively. Five mixed infections of C. suis and Cryptosporidium pig genotype II were detected using PCR/RFLP with genus specific primers. The number of detected mixed infections increased 2.4 fold when a species-specific PCR was employed. No other Cryptosporidium spp. was detected. Unlike cryptosporidiosis of domestic pigs, C. suis was detected as a dominant species infecting adult Eurasian wild boars. There was no association between diarrhoea and the presence of Cryptosporidium infection in the Eurasian wild boars studied. This is the first report on the Cryptosporidium infection caused by C. suis and Cryptosporidium pig genotype II in Eurasian wild boars (Sus scrofa).

  20. Pseudorabies Virus and Brucella abortus from an Expanding Wild Pig ( Sus scrofa ) Population in Southern Oklahoma, USA.

    Science.gov (United States)

    Gaskamp, Joshua A; Gee, Kenneth L; Campbell, Tyler A; Silvy, Nova J; Webb, Stephen L

    2016-04-28

    Wild pigs ( Sus scrofa ) are causing increasing ecologic and economic damage at a global scale. Because wild pigs can carry ≥65 diseases that affect livestock, their widespread expansion threatens native wildlife and livestock. We screened wild pigs from south-central Oklahoma, US for antibodies against Brucella abortus , pseudorabies virus (PRV), and porcine reproductive and respiratory syndrome virus (PRRS). These pathogens were chosen because they are part of eradication programs in the US and could have large economic impacts on domestic livestock if transmitted from wild animals. We tested 282 serum samples during spring 2010 (n=149) and 2011 (n=133) and found an overall exposure rate to PRV of 24.1% (n=68); PRV was detected at two of three study sites. Two wild pigs had detectable antibody to B. abortus , and one had detectable antibody to PRRS. On average, 27% of wild pigs within a sounder were positive for PRV antibody, with 44% of the sounders (16/36) having at least one positive individual. These data highlight that wild pigs could carry pathogens that affect domestic livestock. Because the US is free of these pathogens in commercial livestock operations, continued surveillance and vaccination of domestic livestock are needed. Commercial livestock producers at the wildlife-livestock interface may benefit from spatial prioritization of risk zones to facilitate strategic control efforts.

  1. Levantamento da fauna de Coleoptera que habita a carcaça de Sus scrofa L., em Curitiba, Paraná A study of the Coleoptera (Insecta fauna that inhabits Sus scrofa L. carcass in Curitiba, Paraná

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    Kleber Makoto Mise

    2007-09-01

    Full Text Available Este trabalho visou levantar a fauna de Coleoptera associada à carcaça de Sus scrofa L.,1758, espécie utilizada como modelo em Entomologia Forense. O acréscimo ou a substituição seqüencial das espécies de insetos pode ser utilizado para estimar o intervalo post mortem (IPM. O experimento foi realizado no Centro Politécnico (UFPR, de setembro de 2005 a setembro de 2006. A cada estação foi sacrificado um suíno de 15 kg no local, colocado em gaiola. A captura dos insetos foi realizada diariamente em bandeja posicionada abaixo da carcaça e em armadilha tipo Shannon modificada, e a cada 14 dias em cinco armadilhas do tipo pit-fall. Foram coletados 4.360 Coleoptera, pertencentes a 112 espécies de 26 famílias, 12 consideradas de importância forense. A coleta ativa realizada na bandeja foi responsável pela maior captura (2.023 espécimes, seguida pela armadilha Shannon modificada (2.016 espécimes e por último pelas do tipo pit-fall (324 espécimes. Staphylinidae foi mais coletada na bandeja e Shannon modificada, e Silphidae na armadilha pit-fall. Os principais hábitos encontrados foram predador/parasita (55%e onívoro (38,05%, com poucas espécies consideradas necrófagas (1,31%.This paper sought to assess the Coleoptera fauna associated with carcasses of Sus scrofa L., 1758, which is usually used as model in Forensic Entomology. The addition and sequential substitution of insect species could be used to estimate the post mortem interval (PMI. The present study took place in Centro Politécnico (UFPR, between september 2005 to september 2006. A pig weighting 15 kg was sacrificed each season and put inside a cage. Sampling is made daily in a tray placed below the carcass and in a Shannon modified trap, and each 14 days in five pit-fall traps. 4,360 beetles were collected, belonging to 112 species of 26 families, 12 were considered of forensic potential. The active collecting made in the tray was responsible for the largest number of

  2. Contemporary genetic structure, phylogeography and past demographic processes of wild boar Sus scrofa population in Central and Eastern Europe.

    Science.gov (United States)

    Kusza, Szilvia; Podgórski, Tomasz; Scandura, Massimo; Borowik, Tomasz; Jávor, András; Sidorovich, Vadim E; Bunevich, Aleksei N; Kolesnikov, Mikhail; Jędrzejewska, Bogumiła

    2014-01-01

    The wild boar (Sus scrofa) is one of the most widely distributed mammals in Europe. Its demography was affected by various events in the past and today populations are increasing throughout Europe. We examined genetic diversity, structure and population dynamics of wild boar in Central and Eastern Europe. MtDNA control region (664 bp) was sequenced in 254 wild boar from six countries (Poland, Hungary, Belarus, Ukraine, Moldova and the European part of Russia). We detected 16 haplotypes, all known from previous studies in Europe; 14 of them belonged to European 1 (E1) clade, including 13 haplotypes from E1-C and one from E1-A lineages. Two haplotypes belonged respectively to the East Asian and the Near Eastern clade. Both haplotypes were found in Russia and most probably originated from the documented translocations of wild boar. The studied populations showed moderate haplotype (0.714±0.023) and low nucleotide diversity (0.003±0.002). SAMOVA grouped the genetic structuring of Central and Eastern European wild boar into three subpopulations, comprising of: (1) north-eastern Belarus and the European part of Russia, (2) Poland, Ukraine, Moldova and most of Belarus, and (3) Hungary. The multimodal mismatch distribution, Fu's Fs index, Bayesian skyline plot and the high occurrence of shared haplotypes among populations did not suggest strong demographic fluctuations in wild boar numbers in the Holocene and pre-Holocene times. This study showed relatively weak genetic diversity and structure in Central and Eastern European wild boar populations and underlined gaps in our knowledge on the role of southern refugia and demographic processes shaping genetic diversity of wild boar in this part of Europe.

  3. Two agricultural production data libraries for risk assessment models. [Ovis aries; Capra hircus; Sus scrofa; Gallus domesticus; Meleagris gallopavo

    Energy Technology Data Exchange (ETDEWEB)

    Baes, C.F. III; Shor, R.W.; Sharp, R.D.; Sjoreen, A.L.

    1985-01-01

    Two data libraries based on the 1974 US Census of Agriculture are described. The data packages (AGDATC and AGDATG) are available from the Radiation Shielding Information Center (RSIC), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831. Agricultural production and land-use information by county (AGDATC) or by 1/2 by 1/2 degree longitude-latitude grid cell (AGDATG) provide geographical resolution of the data. The libraries were designed for use in risk assessment models that simulate the transport of radionuclides from sources of airborne release through food chains to man. However, they are also suitable for use in the assessment of other airborne pollutants that can affect man from a food ingestion pathway such as effluents from synfuels or coal-fired power plants. The principal significance of the data libraries is that they provide default location-specific food-chain transport parameters when site-specific information are unavailable. Plant food categories in the data libraries include leafy vegetables, vegetables and fruits exposed to direct deposition of airborne pollutants, vegetables and fruits protected from direct deposition, and grains. Livestock feeds are also tabulated in four categories: pasture, grain, hay, and silage. Pasture was estimated by a material balance of cattle and sheep inventories, forage feed requirements, and reported harvested forage. Cattle (Bos spp.), sheep (Ovis aries), goat (Capra hircus), hog (Sus scrofa), chicken (Gallus domesticus), and turkey (Meleagris gallopavo) inventories or sales are also tabulated in the data libraries and can be used to provide estimates of meat, eggs, and milk production. Honey production also is given. Population, irrigation, and meteorological information are also listed.

  4. Mercury and selenium binding biomolecules in terrestrial mammals (Cervus elaphus and Sus scrofa) from a mercury exposed area.

    Science.gov (United States)

    Ropero, M J Patiño; Fariñas, N Rodríguez; Krupp, E; Mateo, R; Nevado, J J Berzas; Martín-Doimeadios, R C Rodríguez

    2016-06-01

    Mercury (Hg) is likely bound to large biomolecules (e.g. proteins) in living organisms, and in order to assess Hg metabolic pathways and possible toxicological effects, it is essential to study these Hg containing biomolecules. However, the exact nature of most metal binding biomolecules is unknown. Such studies are still in their infancy and information on this topic is scarce because the analysis is challenging, mainly due to their lability upon digestion or extraction from the tissue. New analytical methods that allow complex Hg-biomolecules to be analysed intact are needed and only few very recent studies deal with this approach. Therefore, as an initial step towards the characterization of Hg containing biomolecules, an analytical procedure has been optimised using size-exclusion chromatography (SEC) with inductively coupled plasma mass spectrometry (ICP-MS) detection. We applied this technique to elucidate the distribution and elution profile of Hg and Se, and some physiological important elements such as Fe, Ni, Zn and Cu, to assess metal binding profiles in liver and kidney samples of red deer (Cervus elaphus) and wild boar (Sus scrofa) who roam freely within the largest Hg mining district on Earth, Almadén in Spain. Elemental fractionation profiles of the extracts from different tissues were obtained using two different SEC columns (BioSep-SEC-S2000 GL 300-1kDa and Superdex 75 10/300 GL 70-3kDa). Similar profiles of Hg were observed in red deer and wild boar; however, significant differences were evident for liver and kidney. Moreover, the profiles of Se showed a single peak at high-medium molecular weight in all investigated tissues, while co-elution of Hg with Fe, Ni, Zn and Cu was observed.

  5. Serologic, molecular, and pathologic survey of pseudorabies virus infection in hunted wild boars (Sus scrofa) in Italy.

    Science.gov (United States)

    Verin, Ranieri; Varuzza, Paolo; Mazzei, Maurizio; Poli, Alessandro

    2014-07-01

    To investigate pseudorabies-virus (PrV) -antibody and viral-DNA prevalence, we collected blood, nasal and genital swabs, and tonsillar and lymph-node tissue samples from 139 wild boars (Sus scrofa; 39 piglets, 30 juveniles, and 70 adults), during the hunting season of 2010-2011 in Tuscany, Central Italy. We performed immunohistochemistry with anti-PrV monoclonal antibodies on selected tissue samples. Forty-three of 139 (30.9%) boars were PrV-antibody positive and a 1,954-base-pair PrV-specific product was amplified from nine nasal (6.5%) and 26 genital (18.7%) swabs. Sequence analysis of PrV-positive PCR products revealed identity scores of 99-100% with Suid herpesvirus 1 strain Becker (JF797219) and confirmed the identification of PrV DNA in tested swabs. There was significantly higher antibody prevalence in adults than in juveniles and in piglets than in juveniles. The prevalence of viral DNA was significantly higher in genital swabs than in nasal specimens. The percentage of positive nasal swabs did not differ among age classes. Piglets had a higher percentage of PCR-positive genital swabs than juvenile and adult subjects (30.8% vs. 13.3% and 14.3%, respectively). Results confirmed that PrV infection is widespread in the wild boar population in the study area. The presence of anti-PrV antibodies and of the PrV virus in piglets could be related to vertical transmission of the virus. This hypothesis was also supported by a higher presence of viral genome in genital swabs than in nasal swabs. This field study supports the importance of vertical transmission of PrV, and the high prevalence of virus in genital swabs supports venereal transmission in adult feral boars.

  6. Response of pigmented porcine skin (Sus scrofa domestica) to single 3.8-micron laser radiation pulses

    Science.gov (United States)

    Bostick, Anthony C.; Johnson, Thomas E.; Randolph, Donald Q.; Winston, Golda C. H.

    2005-04-01

    Background and purpose: The purpose of this study is to determine the impact of melanin on skin response to single 3.8 micron, eight microsecond laser pulses and the difference in lesion formation thresholds for input into laser safety standards. Williams et al., performed a study examining laser tissue interaction from 3.8-micron lasers in lightly pigmented Yorkshire pigs (Sus scrofa domestica). However, studies performed by Eggleston et al comparing pigmented and lightly pigmented skin with human skin found that the Yucatan mini-pig is a superior model for laser skin exposures. Methods: Five Yucatan mini-pigs under general anesthesia were exposed to 3.8 micron laser pulses ranging from 0.8 J/cm2 to 93 J/cm2. Gross examinations were done acutely and 24 hours after laser exposure. Skin biopsies were then collected at various times post exposure, and histologic examinations were conducted. Results: The 24 hour ED50 was determined to be 4.5 J/cm2 with fiducial limits of 6.2 and 2.2 J/cm2. As deposited energy was increased, the lesion presentation ranged from whitening of the epidermis (4 J/cm2) to whitening with inflammatory centers (14 J/cm2), and at the highest energy levels inflammatory areas were replaced with an epidermal ulcerated central area (>21 J/cm2). Conclusion: Preliminary findings suggest pigmentation or melanin may play a minor role in the mechanism of laser-tissue damage. The ED50 of Yorkshire pigs was 2.6 J/cm2. The ED50 of the Yucatan mini-pig was found to be 3.6 J/cm2, and although it was higher, it is still within the 95% fiducial limits.

  7. Diagnosis of tuberculosis in the wild boar (Sus scrofa: a comparison of methods applicable to hunter-harvested animals.

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    Nuno Santos

    Full Text Available BACKGROUND: To obtain robust epidemiological information regarding tuberculosis (TB in wildlife species, appropriate diagnostic methods need to be used. Wild boar (Sus scrofa recently emerged as a major maintenance host for TB in some European countries. Nevertheless, no data is available to evaluate TB post-mortem diagnostic methods in hunter-harvested wild boar. METHODOLOGY/PRINCIPAL FINDINGS: Six different diagnostic methods for TB were evaluated in parallel in 167 hunter-harvested wild boar. Compared to bacteriological culture, estimates of sensitivity of histopathology was 77.8%, gross pathology 72.2%, PCR for the MPB70 gene 66.7%, detection of acid-fast bacilli (AFB in tissue contact smears 55.6% and in histopathology slides 16.7% (estimated specificity was 96.7%, 100%, 100%, 94.4% and 100%, respectively. Combining gross pathology with stained smears in parallel increased estimated sensitivity to 94.4% (94.4% specificity. Four probable bacteriological culture false-negative animals were identified by Discriminant Function Analysis. Recalculating the parameters considering these animals as infected generated estimated values for sensitivity of bacteriology and histopathology of 81.8%, gross pathology 72.7%, PCR for the MPB70 gene 63.6%, detection of AFB in tissue contact smears 54.5% and in histopathology slides 13.6% (estimated specificity was 100% for gross pathology, PCR, bacteriology and detection of AFB in histopathology slides, 96.7% for histopathology and 94.4% for stained smears. CONCLUSIONS/SIGNIFICANCE: These results show that surveys for TB in wild boar based exclusively on gross pathology considerably underestimate prevalence, while combination of tests in parallel much improves sensitivity and negative predictive values. This finding should thus be considered when planning future surveys and game meat inspection schemes. Although bacteriological culture is the reference test for TB diagnosis, it can generate false

  8. Identification and Prevalence of Globocephalus samoensis (Nematoda: Ancylostomatidae) among Wild Boars (Sus scrofa coreanus) from Southwestern Regions of Korea.

    Science.gov (United States)

    Ahn, Kyu-Sung; Ahn, Ah-Jin; Kim, Tae-Hoon; Suh, Guk-Hyun; Joo, Kyoung-Woong; Shin, Sung-Shik

    2015-10-01

    This study describes the first record of Globocephalus samoensis (Nematoda: Ancylostomatidae) recovered in wild boars from southwestern regions of Korea. Gastrointestinal tracts of 111 Korean wild boars (Sus scrofa coreanus) hunted from mountains in Suncheon-si, Gwangyang-si, and Boseong-gun between 2009 and 2012 were examined for their visceral helminths. G. samoensis, as identified by morphological characteristics of the head and tail, were recovered from the small intestine of 51 (45.9%) wild boars. Worms were found from 7 of 28 wild boars (25.0%) from Suncheon-si, 40 of 79 (50.6%) from Gwangyang-si, and all 4 (100%) from Boseong-gun. The length of adult females was 7.2±0.5 mm, and the thickest part of the body measured the average 0.47±0.03 mm, while those of males were 6.52±0.19 and 0.37±0.02 mm, respectively. The buccal cavity was equipped with a pair of large and bicuspid subventral lancets near the base of the capsule. The average length of spicules of males was 0.45±0.02 mm. By the present study, G. samoensis is recorded for the first time in southwestern regions of Korea. Additionally, morphological characteristics and identification keys provided in the present study will be helpful in the faunistic and taxonomic studies for strongylid nematodes in both domestic and wild pigs. The infection of G. samoensis apparently did not elicit pathologic lesions, as revealed by macroscopic observation during the autopsy of all wild boars in this study.

  9. Long-term monitoring of 10 selected pathogens in wild boar (Sus scrofa) in Sierra Nevada National Park, southern Spain.

    Science.gov (United States)

    Cano-Manuel, Francisco J; López-Olvera, Jorge; Fandos, Paulino; Soriguer, Ramón C; Pérez, Jesús M; Granados, José E

    2014-11-07

    Wild boar (Sus scrofa) populations are increasing in the Iberian Peninsula, and population management must include disease management and control. In this study, the epidemiology of 10 selected pathogens (Aujeszky's disease virus - ADV, porcine reproductive and respiratory syndrome virus - PRRSV, porcine influenza virus, porcine circovirus, porcine parvovirus, Erysipelotrix rhusiopathiae, Leptospira pomona, Chlamydia/Chlamydiaceae sp., Salmonella sp. and Mycobacterium bovis) in the wild boar population in Sierra Nevada National Park (SNNP), an open unfenced area, is reported, taking into account wild boar population abundance variation in space and time in an open unfenced environment. A total of 1103 wild boar were sampled in 141 hunting events randomly carried out for sampling in seven hunting seasons (October to February from 2002-2003 to 2009-2010 (except 2007-2008). Prevalence was overall lower than those previously reported for fenced wild boar populations in Spain, but all the pathogens analyzed except PRRSV were considered endemic in the SNNP. ADV, E. rhusiopathiae and total pathogen prevalence were positively correlated to wild boar density. Prevalence in the positive areas was significantly higher in females for ADV, E. rhusiopathiae, L. pomona, Chlamydia/Chlamydiaceae sp. and Salmonella sp., and in males for M. bovis. This longitudinal study provides the first data on the health status of the relatively unmanaged and low density wild boar population of SNNP. It is concluded that non-intensively managed wild boar populations are able to maintain the circulation of several pathogens, even in low prevalences and in open unfenced areas with natural density variation both in time and space.

  10. The stress response and exploratory behaviour in Yucatan minipigs (Sus scrofa): Relations to sex and social rank.

    Science.gov (United States)

    Adcock, Sarah J J; Martin, Gerard M; Walsh, Carolyn J

    2015-12-01

    According to the coping styles hypothesis, an individual demonstrates an integrated behavioural and physiological response to environmental challenge that is consistent over time and across situations. Individual consistency in behavioural responses to challenge has been documented across the animal kingdom. Comparatively few studies, however, have examined inter-individual variation in the physiological response, namely glucocorticoid and catecholamine levels, the stress hormones secreted by the hypothalamic-pituitary-adrenal axis and the sympathetic nervous system, respectively. Variation in coping styles between individuals may be explained in part by differences in social rank and sex. Using 20 Yucatan minipigs (Sus scrofa) we: (1) investigated the existence of consistent inter-individual variation in exploratory behaviour and the hormonal stress response, and tested for correlations as predicted by the coping styles hypothesis; and (2) evaluated whether inter-individual behavioural and hormonal variation is related to social rank and sex. Salivary stress biomarkers (cortisol, alpha-amylase, chromogranin A) were assessed in the presence and absence of a stressor consisting of social isolation in a crate for 10 min. Principal components analysis on a set of behavioural variables revealed two traits, which we labelled exploratory tendency and neophobia. Neither exploratory tendency nor neophobia predicted the physiological stress response. Subordinate pigs exhibited higher catecholamine levels compared to dominant conspecifics. We observed sex differences in the repeatability of salivary stress markers and reactivity of the stress systems. The results do not provide support for the existence of behavioural-physiological coping styles in pigs. Sex is an important determinant of the physiological stress response and warrants consideration in research addressing behavioural and hormonal variation.

  11. 应用RACE法分离和克隆猪GPX2基因研究%Study on the Cloning and Isolation of sus scrofa GPX2 Gene by RACE Method

    Institute of Scientific and Technical Information of China (English)

    赵华; 周继昌; 李俊刚; 赵莹; 王康宁

    2008-01-01

    [Objective]Using molecular biotechnology to clone the sus scrofa GPX2 gene.[Method]Using total RNA of sus scrofa duodenum as template,degenerated primer pairs were designed according to the homology alignment analysis of GPX2 gone of human,rat,mouse,dog and cattle.A sus scrofa GPX2 gone sequence of 330 bp was obtained by RT-PCR application method.Primes were designed respectively according to the known sequence,sus scrofa GPX2 gene was isolated and cloned by 3-RACE and 5-RACE method and analyzed the gene sequence.[Result]A mRNA sequence of 924 bp was successfully cloned and isolated in this research.This sequence contained complete 3' end and had higher sequence homology with human,monse,catth and dog GPX2 gene,and there was codon called TGA which encoding See on the position of No.114-116 gene.[Conclusion]Sequence alignment analysis showed that the cloned gene was sus scrofa GPX2 gene(NCBI GenBank database,the sequence number was DQ98982).

  12. Investigation on a focus of human trichinellosis revealed by an atypical clinical case after wild-boar (Sus scrofa pork consumption in northern Italy

    Directory of Open Access Journals (Sweden)

    Romano F.

    2011-02-01

    Full Text Available Trichinellosis is one of the most serious foodborne parasitic zoonoses in Europe. Wild carnivorous and omnivorous hosts are the main reservoirs of Trichinella spp. nematodes in nature. In the winter of 2008-2009, an atypical clinical case of trichinellosis occurred for the consumption of pork from a wild boar (Sus scrofa hunted in southwestern Alps in Italy. The symptomatic individual showed delayed development of oedemas in the lower limbs and eosinophilia, which appeared three months after infection. Muscle samples harboured 3.8 larvae/g, which were identified as Trichinella britovi. During the epidemiological investigation, anti-Trichinella IgG were detected in five hunters.

  13. An RNA-based analysis of changes in biodiversity indices in response to Sus scrofa domesticus decomposition.

    Science.gov (United States)

    Bergmann, R C; Ralebitso-Senior, T K; Thompson, T J U

    2014-08-01

    Despite emergent research initiatives, significant knowledge gaps remain of soil microbiology-associated cadaver decomposition. Nevertheless, preliminary studies have shown that the vast diversity and complex interactions of soil microbial communities have great potential for forensic applications such as clandestine grave location and postmortem interval estimation. This study investigated changes in soil bacterial communities during pig (Sus scrofa domesticus) leg decomposition. 16S rRNA, instead of the usually applied 16S rDNA marker, was used to compare the metabolically active bacteria. Total bacterial RNA was extracted from soil samples of three different layers on day 3, 28 and 77 after the shallow burial of a pig leg. The V3 region of the 16S rRNA was amplified, analysed by RT-PCR DGGE, and compared with control soil bacterial community profiles. Statistically significant differences in soil bacterial biodiversity were observed. For the control, bacterial diversity (H') and species richness (S) of the three layers averaged 2.48±0.14 (H') and 18.8±2.5 (S), respectively, while for the test soil increases (p=0.027) were recorded between day 3 (H'=2.71±0.02; S=21.3±2.0) and 28 (H'=3.46±0.32; S=60.3±16.9), particularly in the middle (10-20 cm) and bottom (20-30 cm) soil layers. Between day 28 and 77 the diversity and richness then decreased on average for all three layers (H'=3.43±0.20; S=60.0±17.3) but remained higher than on day 3. Thus, responses in soil bacterial profiles and activity to carcass decomposition, detected and characterised by RNA-based DGGE, could be used together with RNA sequencing data, changes in physico-chemical variables (carbon, nitrogen, phosphorus, temperature, redox potential, water activity and pH) and conventional macroecology markers (e.g. insects and vegetation), to develop a suite of analytical protocols for different forensic scenarios.

  14. Grubbing by wild boars (Sus scrofa L.) and its impact on hardwood forest soil carbon dioxide emissions in Switzerland.

    Science.gov (United States)

    Risch, Anita C; Wirthner, Sven; Busse, Matt D; Page-Dumroese, Deborah S; Schütz, Martin

    2010-11-01

    Interest in soil C storage and release has increased in recent years. In addition to factors such as climate/land-use change, vertebrate animals can have a considerable impact on soil CO(2) emissions. To date, most research has considered herbivores, while the impact of omnivorous animals has rarely been investigated. Our goal was to determine how European wild boars (Sus scrofa L.), large omnivores that consume soil-inhabiting animals and belowground plant parts by grubbing in the soil, affect soil C dynamics. We measured soil respiration (CO(2)), temperature, and moisture on paired grubbed and non-grubbed plots in six hardwood forest stands for a 3-year period and sampled fine root and microbial biomass at the beginning and after 2 years of the study. We also measured the percentage of freshly disturbed forest soil within the larger surroundings of each stand and used this information together with hunting statistics and forest cover data to model the total amount of CO(2) released from Swiss forest soils due to grubbing during 1 year. Soil CO(2) emissions were significantly higher on grubbed compared to non-grubbed plots during the study. On average 23.1% more CO(2) was released from these plots, which we associated with potential alterations in CO(2) diffusion rates, incorporation of litter into the mineral soil and higher fine root/microbial biomass. Thus, wild boars considerably increased the small-scale heterogeneity of soil properties. Roughly 1% of Switzerland's surface area is similar to our sites (boar density/forest cover). Given the range of forest soil disturbance of 27-54% at our sites, the geographic information system model predicted that boar grubbing would lead to the release of an additional 49,731.10-98,454.74 t CO(2) year(-1). These values are relatively small compared to total soil emissions estimated for Swiss hardwood forests and suggest that boars will have little effect on large-scale emissions unless their numbers increase and their

  15. The wild boar (Sus scrofa Linnaeus, 1758) as secondary reservoir of Fasciola hepatica in Galicia (NW Spain).

    Science.gov (United States)

    Mezo, Mercedes; González-Warleta, Marta; Castro-Hermida, José Antonio; Manga-González, M Yolanda; Peixoto, Raquel; Mas-Coma, Santiago; Valero, M Adela

    2013-12-06

    Fasciolosis is an emerging or reemerging human and animal disease in numerous parts of the world. In Galicia (NW, Spain), the wild boar (Sus scrofa) is the main wild ungulate in terms of abundance and distribution. Its population has continuously increased over the past decades and this population growth has been accompanied by a reduction of habitats, so that the wild boar populations encroach more and more frequently onto agricultural lands. The increase of the interface area between livestock and the wild boars frequently involves the sharing of pastures and water sources, so that the circulation of common pathogens is propitiated. This is the first report concerning the importance of the wild boar as a possible reservoir of Fasciola hepatica infection in Spain. Livers from 358 hunted wild boars were analyzed showing that 11.2% were parasitized by F. hepatica, with burdens ranging from 1 to 14 flukes (mean=2.3). Fecal analysis demonstrated that 40.0% of parasitized animals shed F. hepatica eggs with a mean excretion of 6.1 eggs per gram of feces (epg). The presence of coproantigens analyzed by MM3-COPRO ELISA was positive in 62.9% of infected wild boars. After incubation, the percentage of hatched eggs ranged between 41.0% and 90.0% suggesting that the wild boar is very likely to contribute to the environmental contamination with viable parasite eggs. Comparative morphometric data were obtained using a computer image analysis system (CIAS) on the basis of standardized measurements. F. hepatica from cattle, sheep and wild boars from the same geographical area presents a similar body development and gravidity. Our study shows for the first time that the F. hepatica uterus from the wild boar presents an intermediate size between that found in primary reservoir hosts such as cattle and sheep, i.e., the individual potential egg output capacity of the wild boar does not greatly differ from that detected in Galician livestock. These results show that F. hepatica in

  16. Toll-like receptor 4 polymorphism impairing lipopolysaccharide signaling in Sus scrofa, and its restricted distribution among Japanese wild boar populations.

    Science.gov (United States)

    Shinkai, Hiroki; Okumura, Naohiko; Suzuki, Rintaro; Muneta, Yoshihiro; Uenishi, Hirohide

    2012-04-01

    Toll-like receptor 4 (TLR4) responds to lipid A, the active moiety of lipopolysaccharide from gram-negative bacteria, in cooperation with myeloid differentiation protein-2 and plays a vital role in innate immunity. Polymorphisms in TLR4 are associated with changes in susceptibility to various infectious diseases. We previously found seven amino acid polymorphisms in Sus scrofa TLR4. In this study, we showed by luciferase reporter assay that an alteration from cysteine to tryptophan at position 506 (C506W) caused loss of ability to induce nuclear factor-κB activation after lipid A stimulation. This polymorphism was found only in Japanese wild boar (JWB) populations of S. scrofa. Genotyping of TLR4 in different JWB populations revealed that C506W polymorphism was under pressure from purifying selection in a local population (Tajima's D=-0.98; p<0.05). However, in another population, this polymorphism existed at a frequency such that homozygous animals with the W506 alleles seldom appeared. These findings suggest that the C506W polymorphism is under different types of pressure by natural selection between populations, which may reflect differences in residential pathogens or demographic factors.

  17. A Sealers Midden Provides Evidence a Live Pig ( Sus scrofa) was Taken Ashore at Heard Island During the "Elephanting" Industry (1855-1882)

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    van den Hoff, John; Burton, Harry; Robins, Judith

    2012-12-01

    Livestock was often released onto remote Southern Ocean islands as a food source for shipwreck survivors during the industrial whaling and sealing era. Although animals were put ashore at nearby Isles Kerguelen and Crozet, the historical records make no mention of domesticated livestock ever being set ashore at Heard Island between 1855 and 1882. Here we report a pig ( Sus scrofa) mandible discovered amongst other bones and artefacts in an `elephanters' midden found at Spit Bay, Heard Island. The find provides very strong evidence a live pig was shipped ashore and eaten as part of the sealers meagre provisions. Archaeological investigations of middens at other sealing locations could produce new insights into the dietary habits of these men.

  18. Novel Y-chromosome short tandem repeats in Sus scrofa and their variation in European wild boar and domestic pig populations.

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    Iacolina, L; Brajković, V; Canu, A; Šprem, N; Cubric-Curik, V; Fontanesi, L; Saarma, U; Apollonio, M; Scandura, M

    2016-12-01

    Y-chromosome markers are important tools for studying male-specific gene flow within and between populations, hybridization patterns and kinship. However, their use in non-human mammals is often hampered by the lack of Y-specific polymorphic markers. We identified new male-specific short tandem repeats (STRs) in Sus scrofa using the available genome sequence. We selected four polymorphic loci (5-10 alleles per locus), falling in one duplicated and two single-copy regions. A total of 32 haplotypes were found by screening 211 individuals from eight wild boar populations across Europe and five domestic pig populations. European wild boar were characterized by significantly higher levels of haplotype diversity compared to European domestic pigs (HD  = 0.904 ± 0.011 and HD  = 0.491 ± 0.077 respectively). Relationships among STR haplotypes were investigated by combining them with single nucleotide polymorphisms at two linked genes (AMELY and UTY) in a network analysis. A differentiation between wild and domestic populations was observed (FST  = 0.229), with commercial breeds sharing no Y haplotype with the sampled wild boar. Similarly, a certain degree of geographic differentiation was observed across Europe, with a number of local private haplotypes and high diversity in northern populations. The described Y-chromosome markers can be useful to track male inheritance and gene flow in wild and domestic populations, promising to provide insights into evolutionary and population genetics in Sus scrofa.

  19. Identification of mtDNA lineages of Sus scrofa by multiplex single base extension for the authentication of processed food products.

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    van Asch, Barbara; Silva Santos, Liliana; Carneiro, Joao; Pereira, Filipe; Amorim, Antonio

    2011-07-13

    A genetic method to identify the breed of origin could serve as a useful tool for inspecting the authenticity of the increasing number of monobreed foodstuffs, such as those derived from small local European pig breeds. Mitochondrial DNA (mtDNA) is practically the only reliable genomic target for PCR in processed products, and its haploid nature and strict maternal inheritance greatly facilitate genetic analysis. As a result of strategies that sought to improve the production traits of European pigs, most industrial breeds presently show a high frequency of Asian alleles, while the absence or low frequency of such Asian alleles has been observed in small rustic breeds from which highly prized dry-cured and other traditional products are derived. Therefore, the detection of Asian ancestry would indicate nonconformity in Protected Denomination of Origin products. This study presents a single base extension assay based on 15 diagnostic mtDNA single nucleotide polymorphisms to discriminate between Asian and European Sus scrofa lineages. The test was robust, sensitive and accurate in a wide range of processed foodstuffs and allowed accurate detection of pig genetic material and identification of maternal ancestry. A market survey suggested that nonconformity of products derived from Portuguese breeds is an unusual event at present, but regular surveys both in the local populations and in commercial products would be advisible. Taking into consideration the limitations presented by other methodologies, this mtDNA-based test probably attains the highest resolution for the direct genetic test for population of origin in Sus scrofa food products.

  20. Effect of Capreolus capreolus and Sus scrofa excreta on alanine aminotransferase activity in Glechoma hederacea leaves in conditions of Cd pollution

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    O. M. Vasilyuk

    2015-06-01

    Full Text Available The paper reflects the analysis of Cd impact on the total activity (nM pyruvic acid/ml s of alanine aminotransferase (ALT, EC 2.6.1.2 nitrogen metabolism and the content (mg/ml of water-soluble protein fraction (albumin in Glechoma hederacea L. leaves subject, which dominated in the research area (natural floodplain oak with Stellaria holostea L.. Cd was introduced in the form of salts Cd(NO32 in the concentrations of 0.25, 1.25 and 2.50 g/m2, equivalent to Cd in 1, 5 and 10 doses of MAC. The content of doses of MAC of Cd (5 mg/kg soil was taken into account during introduction. We observed the inhibition of the activity of ALT 3–4 times (with adding the Cd salts at a dose of 1 and 5 МAС compared to controls (area without pollution of Cd and excreta of mammals. This stress reaction took place in the protein complex as well. Thus, albumin content was equal to 72% and 80% (with Cd 1 and 5 MAC compared to control (the area without pollution and excretory functions of mammals. It proved nonspecific response to stress. Using excreta of Capreolus capreolus L. and Sus scrofa L. shows the reduction of Cd effects and increasing the metabolic activity of ALT by 41% and 105%, respectively (with adding of Cd 1 MAC compared to control (pollution by Cd at a dose 1 MAC. The effect of Cd 5 MAC is offset (only with the introduction of C. capreolus excreta compared to control (pollution by Cd at a dose 5 MAC. Normalization of the albumin content (with adding of Cd 1 and 5 MAC compared to control (сontrol of Cd at a dose 1 MAC and сontrol of Cd at a dose 5 MAC with using of excreta of C. capreolus was observed. In conditions of Cd at a doze 10 MAC the ALT activity was reduced 2 times with the introduction of excreta of C. capreolus as S. scrofa compared to control (Cd at a dose 10 MAC. The introduction of excreta of S. scrofa compared with C. capreolus restored the albumin content by 10% to the control. Thus, the feasibility of using different biological

  1. Identification of chromosomal locations associated with tail biting and being a victim of tail-biting behaviour in the domestic pig (Sus scrofa domesticus).

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    Wilson, Kaitlin; Zanella, Ricardo; Ventura, Carlos; Johansen, Hanne Lind; Framstad, Tore; Janczak, Andrew; Zanella, Adroaldo J; Neibergs, Holly Louise

    2012-11-01

    The objective of this study was to identify loci associated with tail biting or being a victim of tail biting in Norwegian crossbred pigs using a genome-wide association study with PLINK case-control analysis. DNA was extracted from hair or blood samples collected from 98 trios of crossbred pigs located across Norway. Each trio came from the same pen and consisted of one pig observed to initiate tail biting, one pig which was the victim of tail biting and a control pig which was not involved in either behaviour. DNA was genotyped using the Illumina PorcineSNP60 BeadChip whole-genome single-nucleotide polymorphism (SNP) assay. After quality assurance filtering, 53,952 SNPs remained comprising 74 animals (37 pairs) for the tail biter versus control comparison and 53,419 SNPs remained comprising 80 animals (40 pairs) for the victim of tail biting versus control comparison. An association with being a tail biter was observed on Sus scrofa chromosome 16 (SSC16; p = 1.6 × 10(-5)) and an unassigned chromosome (p = 3.9 × 10(-5)). An association with being the victim of tail biting was observed on Sus scrofa chromosomes 1 (SSC1; p = 4.7 × 10(-5)), 9 (SSC9; p = 3.9 × 10(-5)), 18 (SSC18; p = 7 × 10(-5) for 9,602,511 bp, p = 3.4 × 10(-5) for 9,653,881 bp and p = 5.3 × 10(-5) for 29,577,783 bp) and an unassigned chromosome (p = 6.1 × 10(-5)). An r(2) = 0.96 and a D' = 1 between the two SNPs at 9 Mb on SSC18 indicated extremely high linkage disequilibrium, suggesting that these two markers represent a single locus. These results provide evidence of a moderate genetic association between the propensity to participate in tail-biting behaviour and the likelihood of becoming a victim of this behaviour.

  2. El jabalí europeo (Sus scrofa: Un invasor biológico como presa reciente del puma (Puma concolor en el sur de Chile The European wild boar (Sus scrofa: A biological invader as a recent prey of the American puma (Puma concolor in southern Chile

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    OSCAR SKEWES

    2012-06-01

    Full Text Available Se estudió la dieta del puma (Puma concoloren los anos 1988 y 2004 en los faldeos de los volcanes Mocho y Choshuenco, pluviselva valdiviana, sur de Chile, a través de la identificación de ítemes-presas en sus heces y la búsqueda en terreno de carcasas de jabalí europeo (Sus scrofa.Se registra por primera vez al jabalí europeo entre los ítemes-presas del puma en Chile. El puma depredó predominantemente sobre juveniles y los porcentajes de consumo variaron entre un 17-37 % dependiendo del método empleado para analizar el contenido de presas presentes en sus heces.The diet of the American puma (Puma concolorwas studied in 1988 and 2004 in the foothills of the volcanoes Mocho and Choshuenco, Valdivian rainforest, southern Chile, through the identification of prey-items in their feces and field surveys of European wild boar (Sus scrofacarcasses. We reported for the first time the invader European wild boar as a puma's prey in Chile. The puma preys mainly on juveniles and its percentage of consumed prey ranges between 17 and 37 % according to the method employed to assess the analyses of their feces prey contents.

  3. Comprehensive analysis of long non-coding RNAs highlights their spatio-temporal expression patterns and evolutional conservation in Sus scrofa

    Science.gov (United States)

    Tang, Zhonglin; Wu, Yang; Yang, Yalan; Yang, Yu-Cheng T.; Wang, Zishuai; Yuan, Jiapei; Yang, Yang; Hua, Chaoju; Fan, Xinhao; Niu, Guanglin; Zhang, Yubo; Lu, Zhi John; Li, Kui

    2017-01-01

    Despite modest sequence conservation and rapid evolution, long non-coding RNAs (lncRNAs) appear to be conserved in expression pattern and function. However, analysis of lncRNAs across tissues and developmental stages remains largely uncharacterized in mammals. Here, we systematically investigated the lncRNAs of the Guizhou miniature pig (Sus scrofa), which was widely used as biomedical model. We performed RNA sequencing across 9 organs and 3 developmental skeletal muscle, and developed a filtering pipeline to identify 10,813 lncRNAs (9,075 novel). Conservation patterns analysis revealed that 57% of pig lncRNAs showed homology to humans and mice based on genome alignment. 5,455 lncRNAs exhibited typical hallmarks of regulatory molecules, such as high spatio-temporal specificity. Notably, conserved lncRNAs exhibited higher tissue specificity than pig-specific lncRNAs and were significantly enriched in testis and ovary. Weighted co-expression network analysis revealed a set of conserved lncRNAs that are likely involved in postnatal muscle development. Based on the high degree of similarity in the structure, organization, and dynamic expression of pig lncRNAs compared with human and mouse lncRNAs, we propose that these lncRNAs play an important role in organ physiology and development in mammals. Our results provide a resource for studying animal evolution, morphological complexity, breeding, and biomedical research. PMID:28233874

  4. First reports of pseudorabies and winter ticks (Dermacentor albipictus) associated with an emerging feral swine (Sus scrofa) population in New Hampshire.

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    Musante, Anthony R; Pedersen, Kerri; Hall, Parker

    2014-01-01

    The expansion of feral swine (Sus scrofa) populations into new geographic regions is of concern not only due to increased range but also because they carry diseases and parasites that pose a threat to humans, livestock, and wildlife into new areas. Recently, emerging feral swine populations have been reported in the northeastern US and due to their adaptive nature will likely continue to spread. During 2009-2012, 49 feral swine were removed from three counties in New Hampshire. Of these, serum samples were submitted from 34 for disease surveillance testing. One of the feral swine was antibody-positive for pseudorabies virus (PRV) making it the first documented infection in feral swine in New Hampshire. Infestations of winter tick (Dermacentor albipictus) were also documented on two of the feral swine which had only been reported previously on feral swine in Texas. Feral swine may not only serve as an important host for an economically important commercial swine pathogen like PRV, but they could also increase host diversity for parasites such as the winter tick, a species that can regionally impact moose (Alces alces) survival. These findings warrant further investigation of expanding and established feral swine populations in New Hampshire as pathogen hosts and support continued effort to reduce numbers or regionally eradicate feral swine.

  5. ORIGENS, RAMIFICAÇÕES E DISTRIBUIÇÕES DAS ARTÉRIAS FACIAIS EM SUÍNOS (SUS SCROFA DOMESTICUS – LINNAEUS, 1758 DA LINHAGEM SADIA

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    Rúbia Aparecida Castilho Maia

    2014-01-01

    Full Text Available The origins, ramifications and distributions of the left and right facial arteries were studied in 30 stillborn pigs (Sus scrofa domesticus of the Sadia lineage, 17 males and 13 females, with the aim of increasing morphological knowledge of these structures in terms of their localization and distribution. The species was chosen for its well known commercial importance. The specimen had its artery system filled with stained solution of neoprene latex 601 A and afterwards fixed in an aqueous solution of formaldehyde at 10%. It was observed that in 100% of the studied animals the facial arteries originated from the ventrolateral surface of the external carotid artery, rostrally to the lingual artery. The gland branches divided, supplying the mandibular, parotid, monostomática portion of the sublingual and part of the cervical thymus in muscular branches, irrigating the medial pterygoid, masseter, ventral aspect of the digastrics and cutaneous of the face muscles. Furthermore, the pharyngal branch irrigated the soft palate, tympanic bula and basihyoid, and the submentalis artery was directed towards the submental and the dorsal face of the digastrics muscles.

  6. Development of a rapid high-efficiency scalable process for acetylated Sus scrofa cationic trypsin production from Escherichia coli inclusion bodies.

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    Zhao, Mingzhi; Wu, Feilin; Xu, Ping

    2015-12-01

    Trypsin is one of the most important enzymatic tools in proteomics and biopharmaceutical studies. Here, we describe the complete recombinant expression and purification from a trypsinogen expression vector construct. The Sus scrofa cationic trypsin gene with a propeptide sequence was optimized according to Escherichia coli codon-usage bias and chemically synthesized. The gene was inserted into pET-11c plasmid to yield an expression vector. Using high-density E. coli fed-batch fermentation, trypsinogen was expressed in inclusion bodies at 1.47 g/L. The inclusion body was refolded with a high yield of 36%. The purified trypsinogen was then activated to produce trypsin. To address stability problems, the trypsin thus produced was acetylated. The final product was generated upon gel filtration. The final yield of acetylated trypsin was 182 mg/L from a 5-L fermenter. Our acetylated trypsin product demonstrated higher BAEE activity (30,100 BAEE unit/mg) than a commercial product (9500 BAEE unit/mg, Promega). It also demonstrated resistance to autolysis. This is the first report of production of acetylated recombinant trypsin that is stable and suitable for scale-up.

  7. Lung parasites of the genus Metastrongylus Molin, 1861 (Nematoda: Metastrongilidae) in wild boar (Sus scrofa L., 1758) in Central-Italy: An eco-epidemiological study.

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    Poglayen, Giovanni; Marchesi, Barbara; Dall'Oglio, Giulia; Barlozzari, Giulia; Galuppi, Roberta; Morandi, Benedetto

    2016-02-15

    The respiratory tracts of 57 wild boars (Sus scrofa L. 1758) hunted in central Italy during the 2011/2012 hunting season were examined to detect the presence of lung worms. Fifty-five out of 57 animals (96,5%) were positive. Five species of Metastrongylus were detected and their prevalence was as follows: Metastrongylus asymmetricus Noda, 1973 (91.2%), Metastrongylus confusus Jansen, 1964 and Metastrongylus salmi Gedoelst, 1923 (87.7%), Metastrongylus apri Gmelin, 1790 (80.7%), Metastrongylus pudendotectus Vostokov, 1905 (70.2%). In most cases multi-species infection was observed. The highest parasite load was found in young animals (<1 year old). The Metastrongylus genus sex ratio (M/F) had a range from 1:4.8 to 1:1.5 in favor of females. The Simpson and Shannon-Wiener indices showed a moderate uniformity in parasite community composition. The Fager index highlighted a high degree of affinity among all pairs of selected parasites. The whole parasite population showed an aggregate distribution. Our findings confirm that these parasites are widespread in the wild boar population. The establishment of outdoor domestic pig farming in the same area of the game preserve could pose the risk of infection to domestic animals. Further studies will be needed to understand the factors involved in the presence and prevalence of the intermediate host as well as the population dynamics of Metastrongylus spp.

  8. Comprehensive analysis of long non-coding RNAs highlights their spatio-temporal expression patterns and evolutional conservation in Sus scrofa.

    Science.gov (United States)

    Tang, Zhonglin; Wu, Yang; Yang, Yalan; Yang, Yu-Cheng T; Wang, Zishuai; Yuan, Jiapei; Yang, Yang; Hua, Chaoju; Fan, Xinhao; Niu, Guanglin; Zhang, Yubo; Lu, Zhi John; Li, Kui

    2017-02-24

    Despite modest sequence conservation and rapid evolution, long non-coding RNAs (lncRNAs) appear to be conserved in expression pattern and function. However, analysis of lncRNAs across tissues and developmental stages remains largely uncharacterized in mammals. Here, we systematically investigated the lncRNAs of the Guizhou miniature pig (Sus scrofa), which was widely used as biomedical model. We performed RNA sequencing across 9 organs and 3 developmental skeletal muscle, and developed a filtering pipeline to identify 10,813 lncRNAs (9,075 novel). Conservation patterns analysis revealed that 57% of pig lncRNAs showed homology to humans and mice based on genome alignment. 5,455 lncRNAs exhibited typical hallmarks of regulatory molecules, such as high spatio-temporal specificity. Notably, conserved lncRNAs exhibited higher tissue specificity than pig-specific lncRNAs and were significantly enriched in testis and ovary. Weighted co-expression network analysis revealed a set of conserved lncRNAs that are likely involved in postnatal muscle development. Based on the high degree of similarity in the structure, organization, and dynamic expression of pig lncRNAs compared with human and mouse lncRNAs, we propose that these lncRNAs play an important role in organ physiology and development in mammals. Our results provide a resource for studying animal evolution, morphological complexity, breeding, and biomedical research.

  9. Functional characterization of a novel lytic phage EcSw isolated from Sus scrofa domesticus and its potential for phage therapy.

    Science.gov (United States)

    Easwaran, Maheswaran; Paudel, Sarita; De Zoysa, Mahanama; Shin, Hyun-Jin

    2015-06-01

    In this study, multi-drug resistant Escherichia coli Sw1 (E. coli Sw1) and active lytic phage EcSw was isolated from feces samples of Sus scrofa domesticus (piglet) suffering from diarrhea. Transmission electron microscopy (TEM) indicated that isolated EcSw belongs to the Myoviridae family with an icosahedral head (80 ± 4) and a long tail (180 ± 5 nm). The EcSw phage genome size was estimated to be approximately 75 Kb of double-stranded DNA (dsDNA). Phage dynamic studies show that the latent period and burst size of EcSw were approximately 20 min and 28 PFU per cell, respectively. Interestingly, the EcSw phage can tolerate a wide range of environmental conditions, such as temperature, pH and ions (Ca(2+) and Mg(2+)). Furthermore, genome sequence analysis revealed that the lytic genes of the EcSw phage are notably similar to those of enterobacteria phages. In addition, phage-antibiotic synergy has notable effects compared with the effects of phages or antibiotics alone. Inhibition of E. coli Sw1 and 0157:H7 strains showed that the limitations of host specificity and infectivity of EcSw. Even though, it has considerable potential for phage therapy for handling the problem of the emergence of multidrug resistant pathogens.

  10. Development of a two-parameter slit-scan flow cytometer for screening of normal and aberrant chromosomes: application to a karyotype of Sus scrofa domestica (pig)

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    Hausmann, Michael; Doelle, Juergen; Arnold, Armin; Stepanow, Boris; Wickert, Burkhard; Boscher, Jeannine; Popescu, Paul C.; Cremer, Christoph

    1992-07-01

    Laser fluorescence activated slit-scan flow cytometry offers an approach to a fast, quantitative characterization of chromosomes due to morphological features. It can be applied for screening of chromosomal abnormalities. We give a preliminary report on the development of the Heidelberg slit-scan flow cytometer. Time-resolved measurement of the fluorescence intensity along the chromosome axis can be registered simultaneously for two parameters when the chromosome axis can be registered simultaneously for two parameters when the chromosome passes perpendicularly through a narrowly focused laser beam combined by a detection slit in the image plane. So far automated data analysis has been performed off-line on a PC. In its final performance, the Heidelberg slit-scan flow cytometer will achieve on-line data analysis that allows an electro-acoustical sorting of chromosomes of interest. Interest is high in the agriculture field to study chromosome aberrations that influence the size of litters in pig (Sus scrofa domestica) breeding. Slit-scan measurements have been performed to characterize chromosomes of pigs; we present results for chromosome 1 and a translocation chromosome 6/15.

  11. Exposures of Sus scrofa to a TASER(®) conducted electrical weapon: no effects on 2-dimensional gel electrophoresis patterns of plasma proteins.

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    Jauchem, James R; Cerna, Cesario Z; Lim, Tiffany Y; Seaman, Ronald L

    2014-12-01

    In an earlier study, we found significant changes in red-blood-cell, leukocyte, and platelet counts, and in red-blood-cell membrane proteins, following exposures of anesthetized pigs to a conducted electrical weapon. In the current study, we examined potential changes in plasma proteins [analyzed via two-dimensional gel electrophoresis (2-DGE)] following two 30 s exposures of anesthetized pigs (Sus scrofa) to a TASER (®) C2 conducted electrical weapon. Patterns of proteins, separated by 2-DGE, were consistent and reproducible between animals and between times of sampling. We determined that the blood plasma collection, handling, storage, and processing techniques we used are suitable for swine blood. There were no statistically significant changes in plasma proteins following the conducted-electrical-weapon exposures. Overall gel patterns of fibrinogen were similar to results of other studies of both pigs and humans (in control settings, not exposed to conducted electrical weapons). The lack of significant changes in plasma proteins may be added to the body of evidence regarding relative safety of TASER C2 device exposures.

  12. Genome-wide identification, classification and functional analyses of the bHLH transcription factor family in the pig, Sus scrofa.

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    Liu, Wuyi

    2015-08-01

    The basic helix-loop-helix (bHLH) transcription factors are one of the largest families of gene regulatory proteins and play crucial roles in genetic, developmental and physiological processes in eukaryotes. Here, we conducted a survey of the Sus scrofa genome and identified 109 putative bHLH transcription factor members belonging to super-groups A, B, C, D, E, and F, respectively, while four members were orphan genes. We identified 6 most significantly enriched KEGG pathways and 116 most significant GO annotation categories. Further comprehensive surveys in human genome and other 12 medical databases identified 72 significantly enriched biological pathways with these 113 pig bHLH transcription factors. From the functional protein association network analysis 93 hub proteins were identified and 55 hub proteins created a tight network or a functional module within their protein families. Especially, there were 20 hub proteins found highly connected in the functional interaction network. The present study deepens our understanding and provided insights into the evolution and functional aspects of animal bHLH proteins and should serve as a solid foundation for further for analyses of specific bHLH transcription factors in the pig and other mammals.

  13. Viral and Antibody Prevalence of Hepatitis E in European Wild Boars (Sus scrofa) and Hunters at Zoonotic Risk in the Latium Region.

    Science.gov (United States)

    Montagnaro, S; De Martinis, C; Sasso, S; Ciarcia, R; Damiano, S; Auletta, L; Iovane, V; Zottola, T; Pagnini, U

    2015-07-01

    Hepatitis E virus (HEV) is a member of the genus Hepevirus within the family Hepeviridae. Hepatitis E is recognized as a zoonosis, and swine and wild boars (Sus scrofa) are known reservoirs of HEV infection. The aim of this study was to investigate the presence of HEV in wild boars and hunters exposed to infection in central Italy (Latium region). During the hunting season, blood samples were collected from 228 wild boars and 20 hunters. The seroprevalence of HEV infection was determined using a commercial enzyme-linked immunosorbent assay, previously validated for use in man, pigs and wild boars. The estimated HEV seroprevalence in wild boars and in hunters was 40.7% (93/228; 95% confidence interval [CI] 34.4-47.1%) and 25% (5/20; 95% CI 6.1-43.9%), respectively. Liver samples were collected from the boars and HEV RNA was detected by nested reverse transcriptase polymerase chain reaction. Fifty-five of 164 tested wild boar liver samples (33.5%; 95% CI 26.2-40.7%) and three of 20 (15.0%; 95% CI 1.3-28.7%) tested human serum samples were positive for HEV RNA. Phylogenetic analysis of the nucleotide sequences obtained from PCR products indicated that the HEV strains present in wild boars and the human population all belonged to genotype 3, supporting the zoonotic role of wild boars in the spread of HEV infection.

  14. Dipterofauna Associated with Sus scrofa Linné, 1758, Carcasses in Urban and Coastal Regions of São Paulo State, Brazil

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    Maria Luiza Cavallari

    2015-01-01

    Full Text Available Cadaverous entomofauna successions vary according to the region, environment, and climate, and such differences may occur within the same country due to seasonal variations. The present study aimed to analyze and compare the dipterofauna that visit or colonize carcasses in the urban and coastal areas of São Paulo, Brazil, during summer and winter seasons. Four swine (Sus scrofa Linné, 1758 carcasses of approximately 12 kg were used. The animals were previously euthanized and then placed in metal cages covered with a flight intercept trap (Shannon, modified. In total, 10,495 flies from 39 families were collected, with 15 species belonging to the Calliphoridae family, 14 species belonging to the Fanniidae family, 43 species belonging to the Muscidae family, and 22 species belonging to the Sarcophagidae family. Flies from these four families visited all carcasses; however, they did not show the highest visitation frequencies in all of the trials. Species variations occurred between the experiments that were performed at different locations and in different seasons. Furthermore, difference in the number of insects attracted to each stage of decomposition was observed. In addition to the four families highlighted above, the families Phoridae, Sepsidae, Otitidae, and Piophilidae were observed in all carcasses.

  15. The CD11a partner in Sus scrofa lymphocyte function-associated antigen-1 (LFA-1: mRNA cloning, structure analysis and comparison with mammalian homologues

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    Thomas Anne VT

    2005-10-01

    Full Text Available Abstract Background Lymphocyte function-associated antigen-1 (LFA-1, CD11a/CD18, alphaLbeta2, the most abundant and widely expressed beta2-integrin, is required for many cellular adhesive interactions during the immune response. Many studies have shown that LFA-1 is centrally involved in the pathogenesis of several diseases caused by Repeats-in-toxin (RTX -producing bacteria. Results The porcine-LFA-1 CD11a (alpha subunit coding sequence was cloned, sequenced and compared with the available mammalian homologues in this study. Despite some focal differences, it shares all the main characteristics of these latter. Interestingly, as in sheep and humans, an allelic variant with a triplet insertion resulting in an additional Gln-744 was consistently identified, which suggests an allelic polymorphism that might be biologically relevant. Conclusion Together with the pig CD18-encoding cDNA, which has been available for a long time, the sequence data provided here will allow the successful expression of porcine CD11a, thus giving the first opportunity to express the Sus scrofa beta2-integrin LFA-1 in vitro as a tool to examine the specificities of inflammation in the porcine species.

  16. Spatio-temporal trends and risk factors for Trichinella species infection in wild boar (Sus scrofa) populations of central Spain: a long-term study.

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    Boadella, M; Barasona, J A; Pozio, E; Montoro, V; Vicente, J; Gortazar, C; Acevedo, P

    2012-07-01

    In south-central Spain, the harvest of Eurasian wild boar (Sus scrofa) has increased significantly during recent decades in association with more intensive management actions to increase hunting yields and with consequent effects on the health status of the wild boar populations. We investigated the spatio-temporal trends and the risk factors related to the prevalence of Trichinella spp. in wild boar in order to obtain the annual probability of occurrence for these parasites in the Ciudad Real province of south-central Spain. Based on muscle samples collected during the hunting seasons from 1998/1999 to 2009/2010, the mean prevalence for Trichinella spp. in 95,070 wild boar was 0.2% (95% confidence interval 0.17-0.23). A subsample of 1,432 wild boar was also tested by ELISA. No correlation was observed between the prevalence of infection detected by serology and by the artificial digestion of muscle. The presence of Trichinella infections in wild boar showed a decreasing trend during the study period and was negatively related with fenced wild boar populations. The predicted 'favourability' for Trichinella infections disappeared almost completely after the 2006/2007 hunting season. Risk maps based on biogeographical tools showed, however, that most hunting estates presented favourable risk factors for these parasites during at least one of the hunting seasons studied.

  17. Towards harmonised procedures in wildlife epidemiological investigations: a serosurvey of infection with Mycobacterium bovis and closely related agents in wild boar (Sus scrofa) in Switzerland.

    Science.gov (United States)

    Beerli, Olivia; Blatter, Sohvi; Boadella, Mariana; Schöning, Janne; Schmitt, Sarah; Ryser-Degiorgis, Marie-Pierre

    2015-01-01

    Bovine tuberculosis (bTB) is a (re-)emerging disease in European countries, including Switzerland. This study assesses the seroprevalence of infection with Mycobacterium bovis and closely related agents in wild boar (Sus scrofa) in Switzerland, because wild boar are potential maintenance hosts of these pathogens. The study employs harmonised laboratory methods to facilitate comparison with the situation in other countries. Eighteen out of 743 blood samples tested seropositive (2.4%, CI: 1.5-3.9%) by ELISA, and the results for 61 animals previously assessed using culture and PCR indicated that this serological test was not 100% specific for M. bovis, cross-reacting with M. microti. Nevertheless, serology appears to be an appropriate test methodology in the harmonisation of wild boar testing throughout Europe. In accordance with previous findings, the low seroprevalence found in wild boar suggests wildlife is an unlikely source of the M. bovis infections recently detected in cattle in Switzerland. This finding contrasts with the epidemiological situation pertaining in southern Spain.

  18. First detection of Sarcoptes scabiei from domesticated pig (Sus scrofa) and genetic characterization of S. scabiei from pet, farm and wild hosts in Israel.

    Science.gov (United States)

    Erster, Oran; Roth, Asael; Pozzi, Paolo S; Bouznach, Arieli; Shkap, Varda

    2015-08-01

    In this report we describe for the first time the detection of Sarcoptes scabiei type suis mites on domestic pigs in Israel and examine its genetic variation compared with S. sabiei from other hosts. Microscopic examination of skin samples from S. scabiei-infested pigs (Sus scrofa domesticus) revealed all developmental stages of S. scabiei. To detect genetic differences between S. scabiei from different hosts, samples obtained from pig, rabbits (Orictolagus cuniculus), fox (Vulpes vulpes), jackal (Canis aureus) and hedgehog (Erinaceus concolor) were compared with GenBank-annotated sequences of three genetic markers. Segments from the following genes were examined: cytochrome C oxidase subunit 1 (COX1), glutathione-S-transferase 1 (GST1), and voltage-sensitive sodium channel (VSSC). COX1 analysis did not show correlation between host preference and genetic identity. However, GST1 and VSSC had a higher percentage of identical sites within S. scabiei type suis sequences, compared with samples from other hosts. Taking into account the limited numbers of GST1 and VSSC sequences available for comparison, this high similarity between sequences of geographically-distant, but host-related populations, may suggest that different host preference is at least partially correlated with genetic differences. This finding may help in future studies of the factors that drive host preferences in this parasite.

  19. Quantitative trait loci for the number of vertebrae onSus scrofa chromosomes 1 and 7 independently inlfuence the numbers of thoracic and lumbar vertebrae in pigs

    Institute of Scientific and Technical Information of China (English)

    ZHANG Long-chao; WANG Li-gang; WANG Li-xian; LIU Xin; LIANG Jing; YAN Hua; ZHAO Ke-bin; LI Na; PU Lei; SHI Hui-bi; ZHANG Yue-bo

    2015-01-01

    Although quantitative trait loci (QTLs) for number of thoracic-lumbar vertebrae have been identiifed onSus scrofa chromo-somes (SSCs) 1 and 7, the inlfuence of these QTLs on the thoracic and lumbar vertebrae is not clear. The aim of this study was to identify single nucleotide polymorphisms (SNPs) associated with total number of thoracic-lumbar vertebrae and for each trait (number of thoracic and lumbar vertebrae) separately. A total of 581 individuals from an F2 Large White×Minzhu population were genotyped using an SNP60K chip. Performing a genome-wide association study (GWAS) for total number of thoracic-lumbar vertebrae, 38 signiifcant SNPs were identiifed in two QTL regions located on SSC1 and SSC7. Performing a GWAS for number of thoracic vertebrae only, 72 signiifcant SNPs were located on SSC7. While performing a GWAS for number of lumbar vertebrae only, 17 signiifcant SNPs were identiifed on SSC1. Gene mining suggested that the gene encoding orphan nuclear receptor, germ cel nuclear factor (NR6A1) on SSC1 was a strong candidate affecting the number of lumbar vertebrae in pigs. Additionaly, genes encoding vertnin (VRTN), prospero homeobox 2 (PROX2), Finkel-Biskis-Jinkins murine osteosarcoma viral oncogene homolog (FOS), and transforming growth factor beta 3 (TGFB3) may be important candidates affecting the number of thoracic vertebrae in pigs. QTLs on SSC1 and SSC7 independently inlfuenced the numbers of tho-racic and lumbar vertebrae. These results shed light on the complex genetic background of vertebrae development in pigs.

  20. Hypoxanthine-guanine phosphoribosyltransferase and inosine 5'-monophosphate dehydrogenase activities in three mammalian species: aquatic (Mirounga angustirostris), semi-aquatic (Lontra longicaudis annectens) and terrestrial (Sus scrofa).

    Science.gov (United States)

    Barjau Pérez-Milicua, Myrna; Zenteno-Savín, Tania; Crocker, Daniel E; Gallo-Reynoso, Juan P

    2015-01-01

    Aquatic and semiaquatic mammals have the capacity of breath hold (apnea) diving. Northern elephant seals (Mirounga angustirostris) have the ability to perform deep and long duration dives; during a routine dive, adults can hold their breath for 25 min. Neotropical river otters (Lontra longicaudis annectens) can hold their breath for about 30 s. Such periods of apnea may result in reduced oxygen concentration (hypoxia) and reduced blood supply (ischemia) to tissues. Production of adenosine 5'-triphosphate (ATP) requires oxygen, and most mammalian species, like the domestic pig (Sus scrofa), are not adapted to tolerate hypoxia and ischemia, conditions that result in ATP degradation. The objective of this study was to explore the differences in purine synthesis and recycling in erythrocytes and plasma of three mammalian species adapted to different environments: aquatic (northern elephant seal) (n = 11), semiaquatic (neotropical river otter) (n = 4), and terrestrial (domestic pig) (n = 11). Enzymatic activity of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) was determined by spectrophotometry, and activity of inosine 5'-monophosphate dehydrogenase (IMPDH) and the concentration of hypoxanthine (HX), inosine 5'-monophosphate (IMP), adenosine 5'-monophosphate (AMP), adenosine 5'-diphosphate (ADP), ATP, guanosine 5'-diphosphate (GDP), guanosine 5'-triphosphate (GTP), and xanthosine 5'-monophosphate (XMP) were determined by high-performance liquid chromatography (HPLC). The activities of HGPRT and IMPDH and the concentration of HX, IMP, AMP, ADP, ATP, GTP, and XMP in erythrocytes of domestic pigs were higher than in erythrocytes of northern elephant seals and river otters. These results suggest that under basal conditions (no diving, sleep apnea or exercise), aquatic, and semiaquatic mammals have less purine mobilization than their terrestrial counterparts.

  1. Hypoxanthine-guanine phosphoribosyltransferase and inosine 5’-monophosphate dehydrogenase activities in three mammalian species: aquatic (Mirounga angustirostris, semiaquatic (Lontra longicaudis annectens and terrestrial (Sus scrofa

    Directory of Open Access Journals (Sweden)

    Myrna eBarjau Perez-Milicua

    2015-07-01

    Full Text Available Aquatic and semiaquatic mammals have the capacity of breath hold (apnea diving. Northern elephant seals (Mirounga angustirostris have the ability to perform deep and long duration dives; during a routine dive, adults can hold their breath for 25 min. Neotropical river otters (Lontra longicaudis annectens can hold their breath for about 30 sec. Such periods of apnea may result in reduced oxygen concentration (hypoxia and reduced blood supply (ischemia to tissues. Production of adenosine 5’-triphosphate (ATP requires oxygen, and most mammalian species, like the domestic pig (Sus scrofa, are not adapted to tolerate hypoxia and ischemia, conditions that result in ATP degradation. The objective of this study was to explore the differences in purine synthesis and recycling in erythrocytes and plasma of three mammalian species adapted to different environments: aquatic (northern elephant seal (n=11, semiaquatic (neotropical river otter (n=4 and terrestrial (domestic pig (n=11. Enzymatic activity of hypoxanthine-guanine phosphoribosyltransferase (HGPRT was determined by spectrophotometry, and activity of inosine 5’-monophosphate dehydrogenase (IMPDH and the concentration of hypoxanthine (HX, inosine 5’-monophosphate (IMP, adenosine 5’-monophosphate (AMP, adenosine 5’-diphosphate (ADP, ATP, guanosine 5’-diphosphate (GDP, guanosine 5’-triphosphate (GTP, and xanthosine 5’-monophosphate (XMP were determined by high-performance liquid chromatography (HPLC. The activities of HGPRT and IMPDH and the concentration of HX, IMP, AMP, ADP, ATP, GTP and XMP in erythrocytes of domestic pigs were higher than in erythrocytes of northern elephant seals and river otters. These results suggest that under basal conditions (no diving, sleep apnea or exercise, aquatic and semiaquatic mammals have less purine mobilization than their terrestrial counterparts.

  2. Utilization of sugarcane habitat by feral pig (Sus scrofa in northern tropical Queensland: evidence from the stable isotope composition of hair.

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    Christopher M Wurster

    Full Text Available Feral pigs (Sus scrofa are an invasive species that disrupt ecosystem functioning throughout their introduced range. In tropical environments, feral pigs are associated with predation and displacement of endangered species, modification of habitat, and act as a vector for the spread of exotic vegetation and disease. Across many parts of their introduced range, the diet of feral pigs is poorly known. Although the remote location and difficult terrain of far north Queensland makes observing feral pig behavior difficult, feral pigs are perceived to seek refuge in World Heritage tropical rainforests and seasonally 'crop raid' into lowland sugarcane crops. Thus, identifying how feral pigs are using different components of the landscape is important to the design of management strategies. We used the stable isotope composition of captured feral pigs to determine the extent of rainforest and sugarcane habitat usage. Recently grown hair (basal hair from feral pigs captured in remote rainforest indicated pigs met their dietary needs solely within this habitat. Stable carbon and nitrogen isotope values of basal hair from feral pigs captured near sugarcane plantations were more variable, with some individuals estimated to consume over 85% of their diet within a sugarcane habitat, while a few consumed as much as 90% of their diet from adjacent forested environments. We estimated whether feral pigs switch habitats by sequentially sampling δ(13C and δ(15N values of long tail hair from a subset of seven captured animals, and demonstrate that four of these individuals moved between habitats. Our results indicate that feral pigs utilize both sugarcane and forest habitats, and can switch between these resources.

  3. Desarrollo y caracterización de cebos para la administración oral de medicamentos a jabalíes (sus scrofa): inmunización oral de rayones con la vacuna bcg frente a la tuberculosis bovina.

    OpenAIRE

    Ballesteros Hurtado, Cristina

    2010-01-01

    El jabalí europeo (Sus scrofa) es un reservorio importante de patógenos como, por ejemplo, aquellos pertenecientes al complejo Mycobacterium tuberculosis que afectan a los seres humanos y a los animales domésticos. La erradicación de estas enfermedades requiere el desarrollo de estrategias de control que reduzcan la transmisión de patógenos entre los animales. Una estrategia adecuada, entre otras, es la vacunación oral. La vacunación efectiva de los jabalíes y otras especies silvestres requie...

  4. Accumulation and distribution of (137)Cs and (90)Sr in the body of the wild boar (Sus scrofa) found on the territory with radioactive contamination.

    Science.gov (United States)

    Gulakov, Andrey Vladimirovich

    2014-01-01

    We studied the concentration and distribution of (137)Cs and (90)Sr in the bodies of 188 wild boar (Sus scrofa Linnaeus, 1758) taken near the Chernobyl site. Of these, 111 animals were taken in the Alienation Zone, 41 animals were taken in the Permanent Control Zone and 36 animals were taken in the Periodic Control Zone. The samples included muscle and bone (rib) tissues and samples of heart, liver, lungs, kidneys, spleen, genitals and skin. The weight of the samples was 0.5 kg fresh weigh. The average concentration of (137)Cs in the muscles of the wild boar found in the Alienation Zone was 46 ± 10 kBq/kg, in the Permanent Control Zone - 13 ± 3.0 kBq/kg and in the Periodic Control Zone - 0.6 ± 0.1 kBq/kg. The largest concentration of (137)Cs was detected in the muscle tissue and kidneys taken animals. In some samples of muscle tissue it reached more than 660 kBq/kg. The (137)Cs concentrations were also high in heart and spleen up 64.3 kBq/kg and 67.5 kBq/kg - animals from the Alienation Zone and 10.3-10.6 kBq/kg - animals from the Permanent Control zone. The lowest concentration of (137)Cs was found in the lungs and skin of animals. The analyses of (90)Sr concentration in the organs and tissues of the wild boar showed that (90)Sr was concentrated mainly in the bone tissue. The average level of (90)Sr concentration in bone was 17.6 kBq/kg fresh weight animals from the Alienation Zone and 13.47 kBg/kg - animals from the Permanent Control zone. In muscle tissues and organs contained (90)Sr - 30.0-110.0 Bq/kg in the Alienation Zone and 11.0-30.0 Bq/kg in the Permanent Control zone.

  5. Simulating the Distribution of Individual Livestock Farms and Their Populations in the United States: An Example Using Domestic Swine (Sus scrofa domesticus) Farms.

    Science.gov (United States)

    Burdett, Christopher L; Kraus, Brian R; Garza, Sarah J; Miller, Ryan S; Bjork, Kathe E

    2015-01-01

    Livestock distribution in the United States (U.S.) can only be mapped at a county-level or worse resolution. We developed a spatial microsimulation model called the Farm Location and Agricultural Production Simulator (FLAPS) that simulated the distribution and populations of individual livestock farms throughout the conterminous U.S. Using domestic pigs (Sus scrofa domesticus) as an example species, we customized iterative proportional-fitting algorithms for the hierarchical structure of the U.S. Census of Agriculture and imputed unpublished state- or county-level livestock population totals that were redacted to ensure confidentiality. We used a weighted sampling design to collect data on the presence and absence of farms and used them to develop a national-scale distribution model that predicted the distribution of individual farms at a 100 m resolution. We implemented microsimulation algorithms that simulated the populations and locations of individual farms using output from our imputed Census of Agriculture dataset and distribution model. Approximately 19% of county-level pig population totals were unpublished in the 2012 Census of Agriculture and needed to be imputed. Using aerial photography, we confirmed the presence or absence of livestock farms at 10,238 locations and found livestock farms were correlated with open areas, cropland, and roads, and also areas with cooler temperatures and gentler topography. The distribution of swine farms was highly variable, but cross-validation of our distribution model produced an area under the receiver-operating characteristics curve value of 0.78, which indicated good predictive performance. Verification analyses showed FLAPS accurately imputed and simulated Census of Agriculture data based on absolute percent difference values of < 0.01% at the state-to-national scale, 3.26% for the county-to-state scale, and 0.03% for the individual farm-to-county scale. Our output data have many applications for risk management of

  6. Simulating the Distribution of Individual Livestock Farms and Their Populations in the United States: An Example Using Domestic Swine (Sus scrofa domesticus Farms.

    Directory of Open Access Journals (Sweden)

    Christopher L Burdett

    Full Text Available Livestock distribution in the United States (U.S. can only be mapped at a county-level or worse resolution. We developed a spatial microsimulation model called the Farm Location and Agricultural Production Simulator (FLAPS that simulated the distribution and populations of individual livestock farms throughout the conterminous U.S. Using domestic pigs (Sus scrofa domesticus as an example species, we customized iterative proportional-fitting algorithms for the hierarchical structure of the U.S. Census of Agriculture and imputed unpublished state- or county-level livestock population totals that were redacted to ensure confidentiality. We used a weighted sampling design to collect data on the presence and absence of farms and used them to develop a national-scale distribution model that predicted the distribution of individual farms at a 100 m resolution. We implemented microsimulation algorithms that simulated the populations and locations of individual farms using output from our imputed Census of Agriculture dataset and distribution model. Approximately 19% of county-level pig population totals were unpublished in the 2012 Census of Agriculture and needed to be imputed. Using aerial photography, we confirmed the presence or absence of livestock farms at 10,238 locations and found livestock farms were correlated with open areas, cropland, and roads, and also areas with cooler temperatures and gentler topography. The distribution of swine farms was highly variable, but cross-validation of our distribution model produced an area under the receiver-operating characteristics curve value of 0.78, which indicated good predictive performance. Verification analyses showed FLAPS accurately imputed and simulated Census of Agriculture data based on absolute percent difference values of < 0.01% at the state-to-national scale, 3.26% for the county-to-state scale, and 0.03% for the individual farm-to-county scale. Our output data have many applications for

  7. Anatomia dos ramos linguais do nervo hipoglosso em Sus scrofa domesticus, L., 1758 - DOI: 10.4025/actascibiolsci.v31i1.772 Anatomy of branches linguais of nerve hipoglosso in sus-scrofa domesticates, L., 1758 - DOI: 10.4025/actascibiolsci.v31i1.772

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    Alessandra Guerra Chaves Dias

    2009-04-01

    Full Text Available Estudamos as ramificações e distribuição do nervo hipoglosso após este penetrar na raiz da língua (36 antímeros de suínos (Sus scrofa domesticus, L. 1758 adultos de ambos os sexos abatidos em frigorífico (região metropolitana de Goiânia, Estado de Goiás - Brasil. O material foi resfriado para transporte (4ºC, fixado em solução aquosa de formaldeído (7% por 72h, imerso em solução aquosa de ácido nítrico (15% por 72h, dissecado sob lupa (RASOR, II-20. Nos antímeros, observou-se o tronco e ramos nervosos primários, secundários e terciários (método de BITTENCOURT et al., 1987. Seguimos a topográfica do nervo, a partir da região retro mandibular, entre as estruturas miofaciais do milohioideo e hipoglosso. Na raiz da língua, o nervo cruza a artéria lingual, passando a ser medial em relação a esta. Subdivide-se em três ordens de grandeza em direção ao músculo longitudinal superior, como segue: antímero esquerdo, quatro a 13 ramos primários, quatro a 21 ramos secundários e zero a 16 ramos terciários; no antímero direito, oito a 18 ramos primários, três a 13 ramos secundários e zero a 12 ramos terciários. Em 27,59% dos antímeros analisados, os ramos do nervo hipoglosso estabeleceram junções com fibras do nervo lingual do antímero correspondente.We studied the ramifications and distribution of the hypoglossal nerve after it penetrated the root of the tongue (36 antimeres of adult swines (Sus scrofa domestica, L. 1758 from both sexes slaughtered in frigorific (metropolitan region of Goiânia, Goiás - Brazil. The material was chilled for transportation (4°C; placed in aqueous solution of formaldehyde (7% for 72 hours; submerged in aqueous solution of nitric acid (15% for 72 hours; dissected under magnifying glass (RASOR, II-20. The trunk and the primary, secondary and tertiary nervous branches were observed in the antimeres (method of Bittencourt et al., 1987. We followed the topography of the nerve starting

  8. Presence of Cryptosporidium scrofarum, C. suis and C. parvum subtypes IIaA16G2R1 and IIaA13G1R1 in Eurasian wild boars (Sus scrofa).

    Science.gov (United States)

    García-Presedo, Ignacio; Pedraza-Díaz, Susana; González-Warleta, Marta; Mezo, Mercedes; Gómez-Bautista, Mercedes; Ortega-Mora, Luis Miguel; Castro-Hermida, José Antonio

    2013-09-23

    The aim of the present study was to identify the species of Cryptosporidium infecting Eurasian wild boars (Sus scrofa) in Galicia (NW, Spain). A sampling of 209 wild boars shot in different game preserves was carried out during the hunting season in 2009-2010. All samples were examined for Cryptosporidium infection, using both immunological and molecular tools. Cryptosporidium oocysts in faecal samples were identified using a direct immunofluorescence technique with monoclonal antibodies (DFA). The presence of Cryptosporidium DNA was determined using nested PCR involving amplification of a fragment of the small-subunit (SSU) ribosomal RNA gene (SSU rRNA). A total of 35 (16.7%) samples tested positive with both techniques. However, sequencing was only possible in 27 samples. Cryptosporidium scrofarum, Cryptosporidium suis and Cryptosporidium parvum oocysts were identified in 19, 5 and 3 of the samples, respectively. Moreover, C. scrofarum was detected as a dominant species infecting all age groups (juveniles, sub adults and adults). Sequence analyses of the glycoprotein (GP60) gene revealed the presence of C. parvum subtypes IIaA16G2R1 in 2 juveniles and IIaA13G1R1 in 1 sub adult wild boar. These species and subtypes have previously been described in human patients, indicating that isolates from asymptomatic wild boars might have zoonotic potential. This is the first report of the presence of C. scrofarum, C. suis and C. parvum subtypes IIaA16G2R1 and IIaA13G1R1 in wild boars (S. scrofa) in Spain.

  9. The role played by sympatric collared peccary (Tayassu tajacu), white-lipped peccary (Tayassu pecari), and feral pig (Sus scrofa) as maintenance hosts for Trypanosoma evansi and Trypanosoma cruzi in a sylvatic area of Brazil.

    Science.gov (United States)

    Herrera, H M; Abreu, U G P; Keuroghlian, A; Freitas, T P; Jansen, A M

    2008-08-01

    The Brazilian Pantanal has been considered one of the richest and most diverse wetland ecosystems in the world. It is occupied by cattle ranching, and a variety of wildlife species share the same habitats with domestic livestock. We investigated infections of Trypanosoma evansi and Trypanosoma cruzi in the sympatric suiformes-collared peccary (Tayassu tajacu), white-lipped peccary (Tayassu pecari), and feral pig (Sus scrofa) by parasitological, serological, and molecular tests. Additionally, we evaluated the health status of both positive and negative suiformes by hematological and biochemical parameters. The results show that peccaries and feral pigs play an important role on the maintenance of both T. evansi and T. cruzi in the Brazilian Pantanal. Health impairment was observed only in the white-lipped peccary infected with T. evansi. Despite presenting low T. evansi parasitemia, all infected white-lipped peccaries displayed low hematocrit values and marked leucopenia. The hematological values showed that the T. evansi infection is more severe in young white-lipped peccaries. The presented data show that feral pigs and peccaries are immersed in the transmission net of both trypanosome species, T. cruzi and T. evansi, in the Pantanal region.

  10. Comparative chromosome painting between the domestic pig (Sus scrofa) and two species of peccary, the collared peccary (Tayassu tajacu) and the white-lipped peccary (T. pecari): a phylogenetic perspective.

    Science.gov (United States)

    Bosma, A A; de Haan, N A; Arkesteijn, G J A; Yang, F; Yerle, M; Zijlstra, C

    2004-01-01

    The Suidae and the Dicotylidae (or Tayassuidae) are related mammalian families, both belonging to the artiodactyl suborder Suiformes, which diverged more than 37 million years ago. Cross-species chromosome painting was performed between the domestic pig (Sus scrofa; 2n = 38), a representative of the Suidae, and two species of the Dicotylidae: the collared peccary (Tayassu tajacu; 2n = 30) and the white-lipped peccary (T. pecari; 2n = 26). G-banded metaphase chromosomes of the two peccaries were hybridized with whole chromosome painting probes derived from domestic pig chromosomes 1-18 and X. For both peccary species, a total of 31 autosomal segments that are conserved between pig and peccary could be identified. The painting results confirm conclusions inferred from G-band analyses that the karyotypes of the collared peccary and the white-lipped peccary are largely different. The karyotypic heterogeneity of the Dicotylidae contrasts with the relative homogeneity among the karyotypes of the Suidae. For this difference between the Dicotylidae and the Suidae, a number of explanations are being postulated: 1) the extant peccaries are phylogenetically less closely related than is usually assumed; 2) the peccary genome is less stable than the genome of the pigs; and 3) special (e.g. biogeographical or biosocial) circumstances have facilitated the fixation of chromosome rearrangements in ancestral dicotylid populations.

  11. A comparative chromosome analysis of Thai wild boar (Sus scrofa jubatus and relationship to domestic pig (S. s. domestica by conventional staining, G-banding and high-resolution technique

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    Pornnarong Siripiyasing

    2007-01-01

    Full Text Available This research is the first comparative chromosome analysis report of Thai wild boar (Sus scrofa jubatus and its relationship to domestic pig (S. s. domestica by conventional staining, G-banding and high-resolution technique. Blood samples of the Thai wild boar were taken from two males and two females kept in Nakhon Ratchasima Zoo. After standard whole blood lymphocyte culture at 37 oC for 72 hr. in the presence of colchicine, the metaphase spreads were performed on microscopic slides and airdried. Conventional staining, G-banding and high-resolution technique were applied to stain the chromosomes. The results showed that the number of diploid chromosomes of Thai wild boar was 2n (diploid = 38, and the fundamental numbers (NF were 62 in the male and female. The type of autosomes were 12 metacentric, 14 submetacentric, 4 acrocentric and 6 telocentric chromosomes, with X and Y chromosomes being metacentric chromosomes. We found that chromosomes 1, 5, 7, 8, 10, 11, 12, 13, 14, 16, 17, 18, X and Y had the same Gbanding and high-resolution technique patterns as those of domestic pig chromosomes. Chromosomes 2, 3, 4, 6, 9 and 15 are similar to those of domestic pig chromosomes. These results show the evolutionary relationship between the Thai wild boar and the domestic pig.

  12. Cerdos y control social de pobres en la provincia de Antioquia, siglo XVIII

    Directory of Open Access Journals (Sweden)

    Mauricio Alejandro Gómez Gómez

    2016-01-01

    Full Text Available Los cabildos de Antioquia, durante el siglo xviii, instaban a los habitantes de ciudades y villas a matar los cerdos callejeros. Este rasgo de violencia permite acercarse a la confrontación cotidiana entre la élite y los criadores de cerdos, un grupo social bajo. Los cabildos defendían un modelo de civilidad basado en estrategias de control social impulsadas por la administración borbónica. Los criadores de puercos, pobres en su mayoría, actuaban como colectivo defendiendo esta actividad, de donde obtenían unos pesos para subsistir. Con el frecuente ataque a los cerdos urbanos, y a sus dueños, la élite quiso impedir que ese sector de pobres adquiriera independencia económica, pues convenía sujetarlos, ocupando su mano de obra en labores mineras, agrícolas y ganaderas.

  13. Efecto de dos sistemas de simulación de monta durante la I. A. en el comportamiento reproductivo de las cerdas (Sus scrofa domestica

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    J. Castañeda

    2004-01-01

    Full Text Available Los estímulos del verraco alrededor de la Inseminación Artificial (IA pueden afectar el desempeño reproductivo de la cerda. El propósito del presente estudio fue evaluar el efecto de alforjas de 25 kg sobre la grupa o un cinturón alrededor de la zona lumbar de las hembras simulando, respectivamente, la monta y presión de las extremidades delanteras del verraco durante la cópula, en el tiempo requerido para realizar la IA, tasa de parición y número de lechones nacidos vivos por cerda. Treinta y tres cerdas primerizas y 117 multíparas alojadas en jaulas individuales desde el momento de la IA hasta el parto, fueron asignadas de manera aleatoria a uno de tres tratamientos: (I animales inseminados artificialmente sin presión sobre sus grupas ni zona lumbar (testigo; (II individuos con alforjas sobre sus grupas durante la IA y (III animales con un cinturón ajustado alrededor de su zona lumbar durante la IA. No se encontró diferencia (P>0.05 en el tiempo requerido para la aplicación del semen durante cada una de las tres inseminaciones realizadas a cada hembra en celo. Las marranas primerizas en el grupo testigo tuvieron mayor (P<0.05 número de lechones nacidos vivos, en comparación con las sometidas a los tratamientos de alforja o cinturón. Se concluye que los aditamentos utilizados durante la IA bajo las condiciones del presente experimento, no mejoran el tiempo requerido para realizar la IA, la tasa de parición, ni el número de lechones nacidos vivos por cerda

  14. Hypoxanthine-guanine phosphoribosyltransferase and inosine 5′-monophosphate dehydrogenase activities in three mammalian species: aquatic (Mirounga angustirostris), semi-aquatic (Lontra longicaudis annectens) and terrestrial (Sus scrofa)

    Science.gov (United States)

    Barjau Pérez-Milicua, Myrna; Zenteno-Savín, Tania; Crocker, Daniel E.; Gallo-Reynoso, Juan P.

    2015-01-01

    Aquatic and semiaquatic mammals have the capacity of breath hold (apnea) diving. Northern elephant seals (Mirounga angustirostris) have the ability to perform deep and long duration dives; during a routine dive, adults can hold their breath for 25 min. Neotropical river otters (Lontra longicaudis annectens) can hold their breath for about 30 s. Such periods of apnea may result in reduced oxygen concentration (hypoxia) and reduced blood supply (ischemia) to tissues. Production of adenosine 5′-triphosphate (ATP) requires oxygen, and most mammalian species, like the domestic pig (Sus scrofa), are not adapted to tolerate hypoxia and ischemia, conditions that result in ATP degradation. The objective of this study was to explore the differences in purine synthesis and recycling in erythrocytes and plasma of three mammalian species adapted to different environments: aquatic (northern elephant seal) (n = 11), semiaquatic (neotropical river otter) (n = 4), and terrestrial (domestic pig) (n = 11). Enzymatic activity of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) was determined by spectrophotometry, and activity of inosine 5′-monophosphate dehydrogenase (IMPDH) and the concentration of hypoxanthine (HX), inosine 5′-monophosphate (IMP), adenosine 5′-monophosphate (AMP), adenosine 5′-diphosphate (ADP), ATP, guanosine 5′-diphosphate (GDP), guanosine 5′-triphosphate (GTP), and xanthosine 5′-monophosphate (XMP) were determined by high-performance liquid chromatography (HPLC). The activities of HGPRT and IMPDH and the concentration of HX, IMP, AMP, ADP, ATP, GTP, and XMP in erythrocytes of domestic pigs were higher than in erythrocytes of northern elephant seals and river otters. These results suggest that under basal conditions (no diving, sleep apnea or exercise), aquatic, and semiaquatic mammals have less purine mobilization than their terrestrial counterparts. PMID:26283971

  15. Reproductive physiology and ovarian folliculogenesis examined via 1H-NMR metabolomics signatures: a comparative study of large and small follicles in three mammalian species (Bos taurus, Sus scrofa domesticus and Equus ferus caballus).

    Science.gov (United States)

    Gérard, Nadine; Fahiminiya, Somayyeh; Grupen, Christopher G; Nadal-Desbarats, Lydie

    2015-01-01

    The aim of this study was to characterize the composition of follicular fluid (FF) collected from the small and large follicles of three mammalian species, Bos taurus, Sus scrofa domesticus, and Equus ferus caballus, that display distinct ovulatory properties. For each species, five large FF samples and five small FF samples were analyzed using 1H-NMR spectroscopy. The FF metabolic profiles of the three species were very distinct. In cows and mares, the metabolic profiles of large FF and small FF were also very distinct. The concentrations of seventeen identified metabolites differed significantly between the sample groups. In mares, fourteen metabolites were found at much greater concentrations in large FF than in small FF (p<0.05). In cows, four metabolites differed in concentration between the large FF and small FF samples (p<0.05). A common feature of the monovulatory species was that the concentrations of α- and β-glucose were much greater in large FF compared with small FF (p<0.05). Sow FF was characterized by the apparent absence of citrate (detected in cow and mare FF), and the presence of succinate (not detected in cow and mare FF). Another obvious difference between species was the concentration of lactate, which was minimal in mare FF compared with cow and sow FF (p<0.05). The findings provide valuable insights into reproductive physiology broadly, and indicate that the activities of central metabolic enzymes differ enormously between these species. Future investigations into species-specific differences in follicle metabolism would increase our understanding of the processes critical to folliculogenesis and the acquisition of oocyte developmental competence.

  16. Investigating the role of free-ranging wild boar (Sus scrofa) in the re-emergence of enzootic pneumonia in domestic pig herds: a pathological, prevalence and risk-factor study.

    Science.gov (United States)

    Batista Linhares, Mainity; Belloy, Luc; Origgi, Francesco C; Lechner, Isabel; Segner, Helmut; Ryser-Degiorgis, Marie-Pierre

    2015-01-01

    Enzootic pneumonia (EP) caused by Mycoplasma hyopneumoniae has a significant economic impact on domestic pig production. A control program carried out from 1999 to 2003 successfully reduced disease occurrence in domestic pigs in Switzerland, but recurrent outbreaks suggested a potential role of free-ranging wild boar (Sus scrofa) as a source of re-infection. Since little is known on the epidemiology of EP in wild boar populations, our aims were: (1) to estimate the prevalence of M. hyopneumoniae infections in wild boar in Switzerland; (2) to identify risk factors for infection in wild boar; and (3) to assess whether infection in wild boar is associated with the same gross and microscopic lesions typical of EP in domestic pigs. Nasal swabs, bronchial swabs and lung samples were collected from 978 wild boar from five study areas in Switzerland between October 2011 and May 2013. Swabs were analyzed by qualitative real time PCR and a histopathological study was conducted on lung tissues. Risk factor analysis was performed using multivariable logistic regression modeling. Overall prevalence in nasal swabs was 26.2% (95% CI 23.3-29.3%) but significant geographical differences were observed. Wild boar density, occurrence of EP outbreaks in domestic pigs and young age were identified as risk factors for infection. There was a significant association between infection and lesions consistent with EP in domestic pigs. We have concluded that M. hyopneumoniae is widespread in the Swiss wild boar population, that the same risk factors for infection of domestic pigs also act as risk factors for infection of wild boar, and that infected wild boar develop lesions similar to those found in domestic pigs. However, based on our data and the outbreak pattern in domestic pigs, we propose that spillover from domestic pigs to wild boar is more likely than transmission from wild boar to pigs.

  17. Wild boars (Sus scrofa scrofa seminiferous tubules morphometry

    Directory of Open Access Journals (Sweden)

    Deiler Sampaio Costa

    2006-09-01

    Full Text Available The aim of this data was to analyze morphology and function of the seminiferous tubule in adult wild boars. Testes removed by unilateral castration of five animals were used. The testicular parenchyma was composed by 82.1±2.2% of seminiferous tubule and 17.9±2.2% of intertubular tissue. The tubular diameter was 249.2±33.0 µm and the seminiferous tubule lenght per gram of testis was 19.3±4.9m. The spermatogonial mitoses efficiency coefficient, meiotic index and spermatogenesis efficiency were 10.34, 2.71 and 30.5 respectively. Each Sertoli cell supported about 13 germinatives cells. The hystometric parameters studied were very similar to those related for domestic boars, however, the wild boars intrinsic efficiency of spermatogenesis and Sertoli cells indexes were smaller than in domestic boars.Objetivou-se com esta pesquisa estudar as características morfométricas e funcionais dos túbulos seminíferos de javalis adultos. Utilizaram-se testículos de cinco animais submetidos a orquiectomia unilateral. O parênquima testicular foi composto por 82,1 ± 2,2% de túbulos seminíferos e 17,9 ± 2,2% de tecido intertubular. O diâmetro tubular foi de 249,2 ± 33,0µm e o comprimento dos túbulos seminíferos por grama de testículo foi de 19,3 ± 4,9m. O coeficiente de eficiência das mitoses espermatogônias, o rendimento meiótico e o rendimento geral da espermatogênese foram, respectivamente, 10,34, 2,71 e 30,50. Cada célula de Sértoli suportou cerca de 13 células germinativas. Conclui-se que os parâmetros histométricos estudados nesta pesquisa foram muito semelhantes aos valores relatados para suínos domésticos, entretanto, o rendimento intrínseco da espermatogênese e os índices de células de Sértoli de javalis foram relativamente baixos quando comparados com aqueles animais.

  18. Avaliação imunofenotípica de subpopulações linfocitárias no sangue do cordão umbilical e periférico de suínos neonatos (Sus scrofa

    Directory of Open Access Journals (Sweden)

    Cesaltina C.M. Tchamo

    2014-04-01

    Full Text Available Considerando a importância do uso do sangue do cordão umbilical como fonte potencial de células tronco hematopoiéticas e o uso do suíno doméstico (Sus scrofa como modelo para pesquisas biomédicas em medicina regenerativa, e por outro lado, visando dar um contributo sobre a quantificação das subpopulações linfocitárias no sangue do cordão umbilical e periférico, objetivou-se quantificar as células CD4+, CD5+ e CD8+ nas amostras de sangue de suínos neonatos. Analisaram-se as amostras do sangue do cordão umbilical e periférico de 48 leitões de linhagem Topigs, provenientes de porcas hígidas, inseminadas artificialmente e de parto natural. Foram coletadas amostras de sangue do cordão umbilical e periférico no momento do nascimento, por meio de venopunção da veia umbilical e seio venoso retro-oftálmico, respectivamente. As quantificações imunofenotípicas de células CD4+, CD5+ e CD8+ foram obtidas por citometria de fluxo. Os valores médios obtidos para as contagens das células CD4+, CD5+ e CD8+ do sangue do cordão umbilical e periférico apresentaram-se inferiores aos reportados para o sangue periférico de suínos adultos, sugerindo um componente imunológico imaturo. A proporção CD4+:CD8+ obtida no sangue do cordão umbilical (3,2±1,2% e no sangue periférico (3,2±1,7% ilustrou a predominância dos linfócitos TCD4+ com relação aos TCD8+. A quantidade relativa de células CD4+ e CD8+ no sangue do cordão umbilical e periférico foi de 1,37±0,86% e 1,15±0,57%, respectivamente.

  19. Chromosomal profile of indigenous pig (Sus scrofa

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    P. Guru Vishnu

    2015-02-01

    Full Text Available Aim: The objective of this study was to investigate the chromosomal profile of indigenous pigs by computing morphometric measurements. Materials and Methods: A cytogenetic study was carried out in 60 indigenous pigs to analyze the chromosomal profile by employing the short term peripheral blood lymphocyte culture technique. Results: The modal chromosome number (2n in indigenous pigs was found to be 38 and a fundamental number of 64 as in the exotic. First chromosome was the longest pair, and thirteenth pair was the second largest while Y-chromosome was the smallest in the karyotype of the pig. The mean relative length, arm ratio, centromeric indices and morphological indices of chromosomes varied from 1.99±0.01 to 11.23±0.09, 1.04±0.05 to 2.95±0.02, 0.51±0.14 to 0.75±0.09 and 2.08±0.07 to 8.08±0.15%, respectively in indigenous pigs. Sex had no significant effect (p>0.05 on all the morphometric measurements studied. Conclusion: The present study revealed that among autosomes first five pairs were sub metacentric, next two pairs were sub telocentric (6-7, subsequent five pairs were metacentric (8-12 and remaining six pairs were telocentric (13-18, while both allosomes were metacentric. The chromosomal number, morphology and various morphometric measurements of the chromosomes of the indigenous pigs were almost similar to those established breeds reported in the literature.

  20. Pneumonia enzoótica em javalis (Sus scrofa Enzootic pneumonia in wild boars (Sus scrofa

    Directory of Open Access Journals (Sweden)

    Roselene Ecco

    2009-06-01

    Full Text Available O objetivo desse estudo foi descrever o quadro clínico e epidemiológico, os achados patológicos, bacteriológicos e imuno-histoquímicos de um surto de pneumonia em uma granja de Javalis do Distrito Federal, Brasil. Em um período de cinco meses, morreram 90 javalis. Desses, 63 tinham lesões pulmonares. Clinicamente apresentavam atraso no desenvolvimento corporal, diminuição do apetite, letargia, tosse e dificuldade respiratória, principalmente quando movimentados. Constatou-se elevação da temperatura, 40ºC em média. Na auscultação, havia crepitações e estertores pulmonares de intensidade moderada. As alterações macroscópicas nos pulmões analisados eram típicas de broncopneumonia lobular. As lesões caracterizavam-se por consolidação crânio-ventral na maioria dos pulmões. A coloração variava de difusamente vermelho-escuro a um padrão mosaico (lóbulos vermelho-escuros intercalados por lóbulos cinzas ou difusamente acinzentados. Na maioria dos pulmões observou-se exsudato mucopurulento na luz dos brônquios e fluindo do parênquima. Histologicamente, as alterações eram de broncopneumonia purulenta e histiocitária com focos de necrose. Em alguns animais havia também hiperplasia do BALT e, na maioria dos animais, infiltração linfocítica perivascular e peribronquial. Bordetella bronchiseptica e Streptococcus spp. foram as principais bactérias isoladas. A imuno-histoquímica demonstrou a bactéria Mycoplasma hyopneumoniae no epitélio bronquiolar e bronquial e o DNA desta bactéria foi detectado pela PCR. Este é o primeiro relato de broncopneumonia em Javalis associado à infecção por M. hyopneumoniae.The aim of this paper is to describe the clinical, epidemiological, pathological, bacteriological and immunohistochemical aspects of a pneumonia outbreak in a wild pig farm in the Distrito Federal, Brazil. Ninety wild pigs died in a period of five months, and 63 of these had pulmonary lesions. Clinically, the pigs presented reduced growth rate, anorexia, lethargy, cough and dyspnea, especially after they were moved. High body temperature (40ºC in average was verified in some animals. Auscultation revealed moderate pulmonary crepitation and stertors. Pulmonary gross lesions were typical of lobular bronchopneumonia. Lung lesions were characterized by ventral-cranial consolidation in the majority of the cases. The color of affected pulmonary areas varied from diffuse dark red to mosaic pattern (dark red lobule intercalate by grayish lobule or diffusely grayish. The majority of the lungs had mucopurulent exsudate in the bronchial lumen that also drained from the parenchyma cut surface. Upon microscopy, the changes were characterized by purulent and histiocytic bronchopneumonia with necrotic foci. In some animals, there was BALT hyperplasia associated with perivascular and peribronchial plasma cells and lymphocytes infiltration in most of these cases. Bordetella bronchiseptica and Streptococcus spp. were the most frequently isolated bacteria. Immunohistochemistry evaluation demonstrated Mycoplasma hyopneumoniae on the luminal surface of bronchial and bronchiolar epithelial cells, and the DNA of bacteria was detected by PCR. This is the first report of bronchopneumonia in wild boars associated with M. hyopneumoniae infection.

  1. Comparative Testing of Hemostatic Dressing in a Large Animal Model (Sus Scorofa) with Severe hepatic Injuries

    Science.gov (United States)

    2013-12-02

    hemostatic dressings in a large animal model (Sus scrofa ) with severe hepatic injuries PRINCIPAL INVESTIGATOR (PI) / TRAINING COORDINATOR (TC): Capt...to Date Sus scrofa 36 18 18 Note. Many fewer animals than approved were used because one of the original treatment groups (Lypressin- soaked gauze

  2. Host-Parasite Relationship of Ticks (Acari: Ixodidae and Argasidae) and Feral Pigs (Sus scrofa) in the Nhecolândia Region of the Pantanal Wetlands in Mato Grosso do Sul.

    Science.gov (United States)

    Cançado, P H D; Faccini, J L H; Herrera, H M; Tavares, L E R; Mourão, G M; Piranda, E M; Paes, R C S; Ribeiro, C C D U; Borghesan, T C; Piacenti, A K; Kinas, M A; Santos, C C; Ono, T M; Paiva, F

    2013-01-01

    Feral pigs (S. scrofa) were introduced to the Pantanal region around 200 years ago and the population appears to be in expansion. Its eradication is considered to be impossible. The population of feral pigs in the Pantanal wetlands is currently estimated at one million. Two scientific excursions were organized. The first was conducted during the dry season, when 21 feral pigs were captured and the second was during the wet season, when 23 feral pigs were captured. Ticks were collected and the oviposition and hatching process were studied to confirm the biological success of each tick species. Three tick species were found to be feeding on feral pigs: Amblyomma cajennense, A. parvum, and Ornithodoros rostratus. During the dry season, 178 adult A. cajennense were collected, contrasting with 127 A. cajennense specimens in the wet season. This suggests that the seasonality of these ticks in the Brazilian Pantanal wetlands could be different from other regions. The results indicate that A. parvum and A. cajennense are biologically successful parasites in relation to feral pigs. A. cajennense appears to have adapted to this tick-host relationship, as well as the areas where feral pigs are abundant, and could play a role in the amplification of this tick population.

  3. DETECCIÓN DEL GENOMA DEL VIRUS DE LA HEPATITIS E (VHE EN MUESTRAS DE HECES DE CERDOS EN PLANTAS DE BENEFICIO DE ANTIOQUIA, COLOMBIA

    Directory of Open Access Journals (Sweden)

    J. E. Forero

    2014-01-01

    Full Text Available El Virus de la Hepatitis E (VHE es uno de los agentes causales de enfermedad hepáticaaguda en humanos, aunque también puede inducir hepatitis crónica en pacientes in-munocomprometidos. Existen cuatro genotipos que generan enfermedad en humanos:los genotipos 1 y 2 asociados con brotes epidémicos por consumo de aguas contami-nadas y los genotipos 3 y 4 de trasmisión zoonótica, implicados en brotes esporádicosen países desarrollados donde el cerdo es el principal reservorio. En Colombia existeevidencia serológica de la infección en humanos y cerdos: se ha detectado el genomaviral en hígados de cerdos en plantas de beneficio y expendios de carne; sin embargono se conoce lo suficiente sobre la infección en el país. Con el fin de determinar si loscerdos del departamento de Antioquia (Colombia están excretando VHE en la edad delbeneficio, se obtuvieron 152 muestras de heces de cerdos en cinco plantas de beneficiode distintas regiones del departamento en las que se determinó la presencia del genomaviral por medio RT-PCR. El porcentaje de positividad hallado fue del 26.9% (41/152;se encontró, además, que los cerdos que provenían de las subregiones Norte y Orientede Antioquia tuvieron el menor (11.6% y mayor (58.3% porcentaje de muestraspositivas, respectivamente. Estos resultados indican que los cerdos en el momento desacrificio están excretando el virus a través de sus heces y que el VHE está circulando enlas diferentes subregiones del departamento.

  4. ÓPTIMOS TÉCNICOS Y ECONÓMICOS EN CORTES DE CARNE DE CERDO EN DOS REGIONES DE MÉXICO

    Directory of Open Access Journals (Sweden)

    Samuel Rebollar-Rebollar

    2014-01-01

    Full Text Available El objetivo de este trabajo fue determinar el peso óptimo de venta de la canal de cerdo ( S. scrofa ssp, en función del rendimiento en cortes primarios, secundarios y terciarios. La información se obtu - vo de febrero a junio de 2012 y provino de 50 cerdos (PV 98,6 ± 7,2 kg, edad 150 ± 5 d, sacrificados en el Rastro Mu - nicipal, distribuidos en diez carnicerías, seleccionadas por intención, localizadas en dos regiones del Estado de México; cinco de ellas en Valle de Bravo y cinco en Tejupilco, ambos municipios limítrofes del Estado de México. Los datos se procesaron mediante tres modelos estadísticos no lineales y se ajustaron a funciones de producción con rendimientos decrecientes. Para el modelo de cortes primarios, los nive - les óptimos técnicos (NOT y niveles óptimos económicos (NOE se obtuvieron con un peso en canal de 94,47 y 90,96 kg, con una ganancia de US$ 162,1 y US$ 173,0. En cortes secundarios los NOT y NOE se alcanzaron en 85,4 y 85,3 kg de peso de la canal, con una ganancia de US$ 236,7 y US$ 236,9. Con base en el modelo de cortes terciarios, se logró un NOT y NOE de 82,38 y 82,26 kg, equivalente a una ganan - cia de US$ 217,6 y US$ 217,9. Un mayor peso de la canal de cerdo no necesariamente implicó una mejor ganancia en dinero, la cual se percibe en la venta de cortes secundarios, con un menor peso de la canal.

  5. Rho相关蛋白激酶抑制剂Y-27632对猪诱导多能干细胞冻存和传代效率的影响%Effected of Rho-associated Protein Kinase Inhibitor Y-27632 on Improving the Cryopreserved Survival and Passage of Porcine(Sus scrofa)Induced Pluripotent Stem Cells

    Institute of Scientific and Technical Information of China (English)

    成德; 高星; 李珍珍; 高祎; 刘亚军; 鲍建昌; 王华岩

    2012-01-01

    Pig is used as an important model animal, and induced pluripotent stem cells (iPS cells) have been established in pig now. However, the efficiency of cryopreserved survival and passage is very low. Rho-associated protein kinase (ROCK) inhibitor Y-27632 enhanced the survival of cryopreserved of human embryonic stem (ES) cells, and improved the ES colony formation. In this study, we used Y-27632 for porcine (Sus scrofa) induced pluripotent stem cells. 5 and 10μmol/L Y-27632 was used for cryopreservation and passage of porcine iPS cells, and it suggested that Y-27632 could greatly suppress cryopreserved-induced apoptosis and increase thaw-survival rates of porcine iPS cells. Meanwhile, it was able to help the adhesion of porcine iPS cell in passage when 5 and 10μmol/L Y-27632 was mixed into culture medium, and it also contributed to porcine iPS colonies formation in 24 h. Furthermore, 10 μmol/L Y-27632 would change the morphology of porcine iPS cells, and made the colonies become flat and loose, but it did not affect alkaline phosphatase (AP) activity and the expression level of pluripotenct genes, octamer-binding transcription factor-4 (Oct4), SRY-related high-mobility-group (HMG)-box protein-2 (Sox2) and homeobox transcription factor (Nanog) in porcine iPS cells treated with Y-27632. Besides, PB [Act-RFP] DS, the transposon reporter construct, was introduced into porcine iPS cells through electric transfected, and RFP positive cells were sorted by flow-cytometry. After treated with 10μmol/L Y-27632 in culture medium, these sorted cells were easier to grow. After these sorting porcine iPS cells injected into porcine early embryos through micromanipulation, the cells treated with Y-27632 were easier to integrate into porcine parthenogenetic embryos than that without treated cells. Above all Y-27632 were able to improve the cryopreserved survival and passage of porcine iPS cells, and suppress the apoptosis induced by single cell dissociated and fluorescence

  6. The complete mitochondrial genome of the Yorkshire pig (Sus scrofa).

    Science.gov (United States)

    Xu, Dong; Yang, Hu; Ma, Haiming

    2016-01-01

    This study aims to identify the complete nucleotide sequence of mitochondrial genome in the Yorkshire pig. Sequence analysis indicates that the genome structure is in accordance with other pig breeds, and it contains 22 tRNA genes, 2 ribosomal RNA genes, 13 protein-coding genes and 1 control region (D-loop region). The complete mitochondrial genome sequence of the Yorkshire pig provides an important record set for further study on genetic mechanism.

  7. Occurrences of ochratoxin A in slaughtered wild boar (Sus scrofa

    Directory of Open Access Journals (Sweden)

    Giancarlo Bozzo

    2013-10-01

    Full Text Available Ochratoxins are fungal secondary metabolites that may contaminate a broad variety of foodstuffs, such as grains, vegetables, coffee, dried fruits, beer, wine and meats. Ochratoxins are considered powerful nephrotoxins, carcinogens, teratogens in rats and likely in humans. In 2011, during a programme aimed to survey the presence of ochratoxin A in 35 regularly slaughtered wild boars in Calabria region (Southern Italy, ochratoxin A (OTA was detected in 35 kidneys, 33 urinary bladders, 33 livers and 32 muscles of 35 animals at the following levels: 1.05 ppb (0.1-3.9 ppb, 0.5 ppb [not detected (ND-2.6 ppb], 0.4 ppb (ND- 2 ppb, 0.2 ppb (ND-0.5 ppb, respectively. A total of 12 samples of kidney, 4 samples of liver, and 4 samples of urinary bladder showed levels of OTA higher than the level (1 ppb established by the guidelines of the Italian Ministry of Health circular No. 10.

  8. Genetic differences in recombination frequency in the pig (Sus scrofa).

    Science.gov (United States)

    Ollivier, L

    1995-10-01

    A comparison has been performed on 3 recently published linkage maps of the pig, hereafter designated as the American (A), European (E), and Swedish (S) maps. The cumulated distances between common markers in these 3 maps were in the ratio 1.00 (A):0.88 (E):0.77 (S), in keeping with the ratio of the percentages of domestic genome in the reference families used to build the corresponding maps, i.e., 1.00 (A):0.81 (E):0.50 (S). From further recombination frequencies reported in wild boars (in the S report), the wild pig genome length (in centimorgans) is expected to represent 66% of the domestic pig genome length. These observations tend to confirm a general result of Burt and Bell (Nature (London), 326: 803-805 (1987)), showing higher chiasma frequencies in domestic mammalian species compared with wild species. Consequences for mapping studies are discussed.

  9. Characterization of a polymorphic IGLV gene in pigs (Sus scrofa).

    Science.gov (United States)

    Schwartz, John C; Murtaugh, Michael P

    2014-08-01

    Swine, unlike other artiodactyls, but similar to humans, utilize both lambda and kappa light chain isotypes almost equally in the generation of their antibody repertoire. The porcine antibody light chain loci have previously been characterized in a single Duroc sow in which was seen extensive allelic variation between light chain genes on homologous chromosomes. However, the extent of variation between individuals is completely unknown. Using deep sequencing of cDNA-derived amplicons from five pigs, we report the identification and characterization of an IGLV gene that is functional and highly expressed in some animals, yet completely absent in others. Our findings provide a possible rationale for the known individual-to-individual variation in antibody responses to vaccination, infectious challenge, and subsequent disease outcome.

  10. Toepasbaarheid in Nederland van afweer- en lokmiddelen voor wilde zwijnen (Sus scrofa scrofa L.).)

    NARCIS (Netherlands)

    Groot Bruinderink, G.W.T.A.

    2008-01-01

    Het Faunafonds wil graag meer inzicht in de beschikbaarheid, kosten, toelaatbaarheid en ervaringen in Duitsland van afweer- en lokmiddelen voor wilde zwijnen, omdat in Nederland de overlast door wilde zwijnen in de afgelopen decennia is toegenomen en omdat traditionele afweermiddelen, zoals wildrast

  11. El cerdo vietnamita: un nuevo caso de mascotas asilvestradas

    OpenAIRE

    Delibes-Mateos, Miguel; Delibes, Adolfo

    2014-01-01

    En lo que es un episodio más de animales exóticos de compañía que acaban creando poblaciones asilvestradas, se ha confirmado que cada vez hay más cerdos vietnamitas viviendo libres en el medio natural español. Se han llegado a detectar hembras preñadas o con crías e incluso posibles híbridos con jabalíes.

  12. 阉割对金华猪肝脏miR-122和miR-378表达量和膻味性状的影响%Effect of Castration on the Boar Taint and the Expression Variation of miR-122 and miR-378 in Liver of Jinhua Pig (Sus scrofa)

    Institute of Scientific and Technical Information of China (English)

    马义涛; 李艳华; 周辉云; 王颖; 徐宁迎

    2013-01-01

    microRNA是一种小分子RNA,是细胞内复杂而精确的调控网络的组成部分.为了研究阉割对miR-122和miR-378表达量的影响以及miR-122和miR-378对雄烯酮和粪臭素代谢的调控作用,本研究利用荧光定量PCR检测了miR-122和miR-378在不同生长阶段金华猪(Sus scrofa)公猪肝脏中的表达量变化及其在阉割和非阉割公猪体内表达量的差异,利用高效液相色谱法(high performance liquid chromatography,HPLC)检测了金华猪皮下脂肪的粪臭素含量,并预测了调控pre-miR-122和pre-miR-378转录的相关转录因子及miR-122和miR-378与雄烯酮、粪臭素代谢相关基因的靶关系.结果发现,miR-122在胚胎期高表达,随着日龄的增加表达量逐渐下降;miR-378在胚胎期高表达,生长期呈现先增后减的态势.阉割后两者的表达量均较同期非阉割组表达下调.并且阉割后皮下脂肪中粪臭素的含量显著下降(P<0.01).根据研究结果推测,阉割后激素水平的变化通过相关转录因子影响microRNA的表达,直接或间接影响雄烯酮和粪臭素代谢而实现对公猪膻味性状的调控.而在这个调控网络中,microRNA可能发挥了重要作用,为深入研究公猪膻味性状提供了一个新的思路.%MicroRNA(miRNA) is a class of small RNA,it is involved in the intracellular complicated and precise regulatory networks.In order to study the effect of castration on the expression of miR-122 and miR-378 and the regulation effect of miR-122 and miR-378 on androstenone and skatole metabolism,we detected the skatole content in subcutaneous fat of boars and barrows with the help of high performance liquid chromatography (HPLC) and the expression of miR-122 and miR-378 in various growth stages in liver of Jinhua Pig (Sus scrofa) by quantitative Real-time PCR (qRT-PCR) and analysed the expression variation between boars and barrows.The results showed that the skatole content in adipose tissue was higher (P<0.01) in boars

  13. Development of a microsatellite-based method for the differentiation of European wild boar (Sus scrofa scrofa) from domestic pig breeds (Sus scrofa domestica) in food.

    Science.gov (United States)

    Conyers, Christine M; Allnutt, Theodore R; Hird, Heather J; Kaye, Joy; Chisholm, James

    2012-04-04

    Twenty microsatellites (simple sequence repeats, SSR) were used to discriminate wild boar from domestic pig and to identify mixtures of the two. Reference groups of wild boar and pig samples were collected from the UK and Europe for genetic assignment tests. Bayesian Analysis of Populations software (BAPs) gave 100% correct assignment for blind wild boar and pig samples and correctly identified mixed samples. DNA was extracted from 12 commercial food samples (11 labeled as containing wild boar) including patés, salamis, and sausage, and good SSR profiles were obtained. Eleven samples were correctly assigned as pig, and two as mixed meats. One sample sold as wild boar meat was clearly assigned as pig. A further 10 blind samples of meat cuts were analyzed, eight wild boar and two pig, and all were correctly assigned.

  14. Lesiones en órganos de cerdos posdestete, inducidas por el lipopolisacárido de E. coli

    Directory of Open Access Journals (Sweden)

    Cristian Gutiérrez V.

    2013-08-01

    Full Text Available Objetivo. Evaluar el efecto de la ingestión de varios niveles de Lipopolisacárido (LPS de E. coli sobre las manifestaciones clínicas y lesiones en órganos de cerdos recién destetados. Materiales y métodos. El trabajo de campo se realizó en el Centro San Pablo, perteneciente a la Universidad Nacional de Colombia. El estudio se realizó con 52 cerdos destetados (6.5±0.5 kg a los 21 días de edad. Los animales fueron alimentados durante 10 días con una dieta basal compuesta de leche y algunos de sus derivados, adicionada con cuatro niveles de LPS (0, 0.3, 0.5 y 1.0 μg/ml de alimento. Los cerdos se sacrificaron escalonadamente los días 1, 5, 7 y 10 posdestete y se tomaron muestras de intestino delgado, estómago, hígado, páncreas, corazón, pulmón, riñón y bazo. El monitoreo clínico y paraclínico se realizó diariamente durante la investigación. Para determinar la ganancia de peso, los animales fueron pesados el día del destete y el día del sacrificio. Resultados. Hubo diferencia (p<0.01 en las variables peso de los órganos y ganancia de peso, donde los animales que consumieron el mayor nivel de LPS presentaron los menores valores, llegando a su mínimo nivel el día 10 posdestete. Las variables presentación de: lesiones macroscópicas, diarreas, y temperatura rectal, aumentaron con el nivel de inclusión de LPS en la dieta, llegando a su máximo nivel el día 10 posdestete (p<0.01. Conclusiones. El LPS de E. coli provoca la inhibición del crecimiento corporal y de los órganos en estudio y una alta incidencia de diarreas.

  15. Ensayo Comparativo Entre el Maíz y la Torta de Copra en el Levante y Engorde de Cerdos

    Directory of Open Access Journals (Sweden)

    Calad U. José Fernando

    1959-06-01

    Full Text Available La necesidad de incrementar la producción de carne de cerdo en el país es de una palpitante actualidad, dado el bajo poder nutritivo de la dieta de nuestro pueblo, problema éste que tiende a agudizarse cada día debido a los altos costos de los elementos esenciales en la alimentación humana, tales como la carne, leche, etc. La industria porcina podría ser una buena solución a este grave problema ya que ella puede convertirse con un poco de estudio y de educación de nuestras gentes en una próspera y fuerte industria familiar, no tan restringida como se encuentra en la actualidad; sería necesario también racionalizar los sistemas de explotación con el fin de hacerlos más productivos. En esta forma, a ¡la par que se ayudaría a resolver el problema de la alimentación, se fortalecería en forma notable la economía nacional mediante el aprovechamiento completo de los productos y subproductos agrícolas que hoy en muchos casos se desperdician lamentablemente. Aparte del mercado nacional, susceptible de un aumento vertical en la demanda, Colombia puede pensar seriamente, por poseer grandes extensiones de terrenos aptos para esta explotación y contar con productos agrícolas de muy buenas características para la alimentación de cerdos, en convertirse en un emporio porcino de primer orden. En la actualidad nuestras explotaciones de ganado de cerda son rudimentarias y enfrentan problemas de diferente índole tales como enfermedades, deficiente alimentación, falta de selección, etc., problemas estos que hacen que la producción de cerdo sea inferior a nuestras necesidades. Pero este hecho que es una de las tantas demostraciones del retraso de nuestras industrias pecuarias, puede ser remediado fácilmente con un programa de capacitación de los productores actuales o potenciales del cerdo. Dadas las circunstancias de que un programa de educación campesina falla cuando es necesario hacer extensión con base en datos

  16. Yuca ensilada como fuente de energía para cerdos en crecimiento

    Directory of Open Access Journals (Sweden)

    Pedro Lezcano Perdigón

    2014-01-01

    Full Text Available Se condujeron dos experimentos para determi - nar el comportamiento de cerdos en crecimiento, comparando la dieta de maíz-soya con otra de raíz de yuca ensilada con agua o vinaza. Se utilizaron 16 cerdos de cruce comercial alojados en corrales individuales, mediante clasificación simple y dos tratamientos con ocho repeticiones/ tratamiento. En el segundo trabajo se utilizaron 16 animales en similares condiciones, con vina - za para ensilar. No fueron encontradas diferen - cias significativas para la ganancia diaria (g y conversión alimentaria (kg kg -1 . Se concluye que la raíz de yuca ensilada con agua y yogurt o vinaza, puede sustituir totalmente la energía del maíz para cerdos en crecimiento.

  17. Aspectos anatomopatológicos del linfosarcoma en el cerdo

    Directory of Open Access Journals (Sweden)

    A. RUIZ

    1997-01-01

    Full Text Available Se describen tres casos de linfosarcoma en cerdos de planteles de crianza industrial, dos de los cuales tenían un peso vivo de 60 kg y el tercer caso correspondió a una hembra reproductora de 280 kg de peso vivo. Los animales no presentaban signos ni síntomas clínicos específicos atribuibles a la enfermedad. A la necropsia se observó: aumento de tamaño de los ganglios linfáticos, bazo, hígado y placas de Peyer; presencia de un puntillado o nodulaciones blancas de tamaño variable en hígado y riñón. Histopatológicamente estos tejidos tenían infiltraciones de células de origen linfoide, y de aspecto de linfosarcoma.Three cases of lymphosarcoma in industrially-bred pigs are described, in which two of the pigs had a live weight of 60 kg, and the third, a reproductive female, had a live weight of 280 kg. The animals did not present any signs, nor specific clinical symptoms attributable to the disease. During the necropsy, an enlargement in the size of the lymphatic nodes, spleen, liver, and Peyer’s Plate was observed, as was the presence of white spots or nodules of various sizes on the liver and kidney. The histopathology carried out on the tissue revealed the presence of cells of lymphoid origin, and of a histological aspect of lymphosarcoma.

  18. CAMBIOS EN LA ACTIVIDAD ENZIMÁTICA EN DUODENO Y YEYUNO DE CERDOS DURANTE VARIOS PERIODOS POSDESTETE

    Directory of Open Access Journals (Sweden)

    D. Ospina

    2011-01-01

    Full Text Available Objetivos: evaluar los cambios en la actividad enzimática intestinal de algunos cerdosdurante varios periodos posdestete. Materiales y métodos: el trabajo de campo serealizó en el Centro San Pablo, perteneciente a la Universidad Nacional de Colombia,sede Medellín. El estudio se realizó con 16 cerdos destetados a los 21 días de edad. Losanimales experimentales fueron alimentados durante 10 días con una dieta basal (DBque tuvo como componentes leche y algunos de sus derivados y que además cumplíacon los requerimientos nutricionales. Luego, los cerdos fueron sacrificados escalonadamentelos días uno, cinco, siete y diez posdestete, y se realizó extracción completa delintestino delgado, el cual fue dividido en dos secciones (duodeno y yeyuno de igualtamaño. El análisis de la actividad enzimática (APN, DPP-IV, LPH, MGA y SI se realizómediante el método de velocidades iniciales y el diseño estadístico que se empleófue completamente al azar, no obstante, los datos se analizaron mediante la aplicaciónde los modelos lineales generales (GLM y de la prueba de duncan. Resultados:se evidencia que las enzimas presentaron diferencias (P < 0,01 durante los periodosposdestete. En el día cinco se observaron cambios negativos en todas las enzimas encomparación con su estado durante el primer día posdestete; sin embargo, entre el díauno y diez posdestete se presentaron diferencias entre APN, DPP-IV y LPH (P < 0,01.Además, se encontró que la sección proximal (duodeno presentó las mayores actividadesenzimáticas (P < 0,01. Conclusiones: el destete temprano disminuye la actividadenzimática a nivel intestinal, provocando la subutilización de los nutrientes de la dietay, por ende, alta incidencia de diarreas durante esta etapa.

  19. Cinética de la infección experimental en cerdos con Mycoplasma hyopneumoniae usando inmunofluorescencia

    OpenAIRE

    2003-01-01

    Se observó por medio de inmunofluorescencia el epitelio bronquial de cerdos infectados experimentalmente con Mycoplasma hyopneumoniae, el evento de colonización se analizó durante los primeros 20 días posinfección. Doce cerdos de un mes de edad fueron inoculados intratraquealmente con un homogeneizado pulmonar conteniendo M. hyopneumoniae. Los cerdos fueron sacrificados de dos en dos, cada uno de los días 1, 4, 8, 12, 16 y 20 posinoculación, así como otros seis animales testigo que fueron ino...

  20. Importancia del bienestar animal en la decisión de compra de carne fresca de cerdo

    OpenAIRE

    Cabana Villar, Jordi

    2010-01-01

    Actualmente en España el nivel de bienestar animal en la producción de cerdos es bajo, ya que al nacer los cerdos se les castra sin anestesia, causando gran dolor al animal. En España se castran 8 millones de cerdos al año, principalmente para mejorar la calidad de la carne, pero también para facilitar el manejo de los mismos. Si los granjeros castraran a los animales con anestesia implicaría costes de producción más elevados al tener que costear el material médico, y el bienes...

  1. Ecología trófica del jabalí europeo (Sus scrofa silvestre en Chile Trophic ecology of the wild boar (Sus scrofa in Chile

    Directory of Open Access Journals (Sweden)

    ÓSCAR SKEWES

    2007-09-01

    Full Text Available Documentamos la dieta del jabalí silvestre en el centro-sur de Chile basados en el examen de 20 estómagos colectados en los faldeos de volcán Mocho-Choshuenco (39°54' S, 72°02' O y en el Parque Nacional Vicente Pérez Rosales (41°03' S, 71°54' O. La dieta está representada por animales, vegetales y hongos. Entre los vegetales y hongos son consumidas tanto las partes epígeas como hipógeas, lo mismo que frutos y semillas. Hongos y rizomas de Gunnera tinctoria constituyen ítemes vegetales cuantitativamente importantes en la alimentación. El jabalí forrajea en general en ambiente boscoso, pero las especies vegetales más frecuentes en la dieta se encuentran en espacios abiertos o fuera del bosque. El ambiente dominado por Chusquea spp. constituye un importante sitio de alimentación. Los roedores de la familia Muridae dominan entre los animales determinados en la dieta del jabalí. Esta situación, sin embargo, parece obedecer a la ocurrencia de una ratada producto de la semillación masiva de Chusquea spp. durante la época de muestreo. Las aves de la familia Rhinocryptidae e invertebrados (en especial larvas del coleóptero Chiasognathus grantii constituyen parte frecuente de los ítemes animales de la dieta del jabalí. En comparación a estudios en otras partes del mundo, el jabalí silvestre en Chile consume hongos en porcentaje de ocurrencia similar (65 % a su dieta en Estados Unidos de América pero bastante más alta que su dieta en Europa (5-32 %. En relación al volumen del componente animal, el jabalí en Chile consume más (16,1 % que en Estados Unidos de América (6 % y que en Europa (7-13 %We document the diet of wild boar in south-central Chile based on analysis of 20 stomachs collected on the outskirts of Mocho-Choshuenco volcano (39°54' S, 72°02' W and of Vicente Pérez Rosales National Park (41°03' S, 71°54' W. The diet incorporates animals, plants and fungi. Among the latter two, both epigeal and hypogeal parts are consumed, together with fruits and seeds. Fungi and rhizomes of Gunnera tinctoria are the most abundant plant items in the diet. The wild boar in Chile forages chiefly in forested environments, but nonetheless the most frequent plant items in its diet are found in open spaces or outside the forest. Environments dominated by bamboos Chusquea spp. constitute important foraging sites. Rodents in the family Muridae prevail among animal items in the wild boar diet. This situation, however, is associated to the occurrence of a mouse outbreak (ratada resulting from the mast seeding of Chusquea spp. during our study. Birds in the family Rhinocryptidae together with invertebrates (specially Coleopteran larvae of Chiasognathus grantii made up a sizeable part of the animals in the wild boar diet. In comparison to elsewhere, the wild boar in Chile consumes fungi in a similar percentage of occurence (65 % as in the United States of America but much higher than in Europe (5-32 %. With regard to the animal component, the wild boar in Chile consumes more (20 % volume of such items than in the United States of America (6 % and in Europe (7-13 %

  2. Influence of nutrient substrates on the expression of cellulases in Cerambyx cerdo L. (Coleoptera: Cerambycidae larvae

    Directory of Open Access Journals (Sweden)

    Pavlović R.

    2012-01-01

    Full Text Available The expression and distribution of digestive cellulases along the midgut of Cerambyx cerdo larvae were analyzed for the first time and are presented in this article. Four groups of larvae were examined: larvae developed in the wild; larvae taken from the wild and successively reared on an artificial diet based on polenta; and larvae hatched in the laboratory and reared on two different artificial diets. Seven endocellulase and seven β-D-glucosidase isoforms were detected in all midgut extracts of C. cerdo with a zymogram after native PAGE. We observed that C. cerdo larvae are capable of producing cellulase isoforms with different PAGE mobilities depending on the nutrient substrate. From our findings it can be assumed that, depending on the distribution of endocellulase and β-D-glucosidase, cellulose molecules are first fragmented in the anterior and middle midgut by endo-β-1,4-glucanase; subsequently, the obtained fragments are broken down by β-D-glucosidase mostly in middle midgut.

  3. Artropofauna de importancia forense en un cadáver de cerdo en el Callao, Perú

    Directory of Open Access Journals (Sweden)

    Iannacone José

    2003-01-01

    Full Text Available This is the first report of an ongoing study on arthropofauna of forensic importance in Callao, Peru using a baby pig (Sus scrofa Linneaus, 1758 on land as a model to determine the arthropofauna over 84 days of weekly survey between 17 July and 02 October 2 000. A total of 4,405 specimens were collected belonging to five orders and eight families: Cochliomyia macellaria (Fabricius, 1775 (Diptera, Calliphoridae (81.62%; Dermestes maculatus (De Geer, 1774 (Coleoptera, Dermestidae (16.35%; Fannia canicularis (Linnaeus, 1761 (Diptera, Muscidae (0.04%; Saprinus aeneus (Fabricius, 1775 (Coleoptera, Histeridae (1.48%; Necrobia rufipes (De Geer, 1775 (Coleoptera, Cleridae (0.45%; Linepithema humile (Mayr, 1868 (Hymenoptera, Formicidae (0.02%; Porcellio laevis Latreille, 1804 (Isopoda, Porcellionidae (0.02% and Hadruroides lunatus (L. Koch, 1867 (Scorpionida, Iuridae (0.02%. Larvae accounted for 76%, pupae 14% and adults 10% of the total collected. The arthropods were into three: necrophages (98.01%, predators (1.95% and omnivorous (0.04%. C. macellaria were significantly higher during the decayed stage; by contrast D. maculatus was much higher in dry remains stage. The highest diversity with the Shannon-Weaver (H' and Pielou (J index were found during the advanced decayed stage. The absence of species of genus Chrysomyia Robineau-Desvoidy, 1830 is discussed.

  4. Interferon-induced Sus scrofa Mx1 blocks endocytic traffic of incoming influenza A virus particles.

    Science.gov (United States)

    Palm, Mélanie; Garigliany, Mutien-Marie; Cornet, François; Desmecht, Daniel

    2010-01-01

    The interferon-induced Mx proteins of vertebrates are dynamin-like GTPases, some isoforms of which can additionally inhibit the life cycle of certain RNA viruses. Here we show that the porcine Mx1 protein (poMx1) inhibits replication of influenza A virus and we attempt to identify the step at which the viral life cycle is blocked. In infected cells expressing poMx1, the level of transcripts encoding the viral nucleoprotein is significantly lower than normal, even when secondary transcription is prevented by exposure to cycloheximide. This reveals that a pretranscriptional block participates to the anti-influenza activity. Binding and internalization of incoming virus particles are normal in the presence of poMx1 but centripetal traffic to the late endosomes is interrupted. Surprisingly but decisively, poMx1 significantly alters binding of early endosome autoantigen 1 to early endosomes and/or early endosome size and spatial distribution. This is compatible with impairment of traffic of the endocytic vesicles to the late endosomes.

  5. Expression patterns and subcellular localization of porcine (Sus Scrofa) lectin,galactose-binding, soluble 1 gene

    Institute of Scientific and Technical Information of China (English)

    Haifang Qiu; Shuhong Zhao; Mei Yu; Bin Fan; Bang Liu

    2008-01-01

    Lectin,galactose-binding,soluble 1 (LGALS1) gene encodes galectin-1,an atypical secretory protein that plays an important role during myoblast proliferation and differentiation.In this study,the porcine LGALS1 gene was cloned and characterized from pig muscle.The predicted protein sequence shared a high identity with its mammalian counterparts.Reverse transcription-polymerase chain reaction revealed that porcine LGALS1 was expressed at 33 day post-coitus (dpc) and 65 dpc at a relatively high level,and then decreased to 90 dpc during fetal skeletal muscle development,suggesting that galectin-1 is a potent factor implicated in the formation of myofibers.LGALS1 was found widely expressed in all tissues and transient transfection indicated that galectin-1 locates both in cytoplasm and nucleus.Genomic sequences and analysis predicted a promoter region at approximately 1.279.1.529 kb,but dualluciferase reporter assay indicated that it has little promoter activity.

  6. First detection of sarcoptic mange in free-ranging wild boar (Sus scrofa) in Switzerland.

    Science.gov (United States)

    Haas, C; Origgi, F C; Akdesir, E; Batista Linhares, M; Giovannini, S; Mavrot, F; Casaubon, J; Ryser-Degiorgis, M-P

    2015-05-01

    In Switzerland sarcoptic mange is frequent in free-ranging wild carnivores but until recent years no cases had been recorded in wild ungulates. Since 2010, cases have been observed in wild boar in the cantons of Solothurn, Tessin and Thurgau. Here, we report the detection of mange-like skin lesions in wild boars by photo-trapping and the post-mortem findings in 6 culled animals presenting different stages of the disease. Potential sources of infection include mangy red foxes, outdoor domestic pigs and wild boars from surrounding countries. Disease spread in the wild boar population may become relevant not only for wildlife but also for domestic pig health in the future if piggeries' biosecurity is insufficient to prevent interactions with wild boar.

  7. The complete mitochondrial genome sequence of Diannan small-ear pig (Sus Scrofa).

    Science.gov (United States)

    Wang, Ling-Yu; Xu, Dong; Xiao, Ding-Fu; Ma, Hai-Ming

    2016-01-01

    In this study, the complete mitochondrial genome sequence of Diannan small-ear pig in Yunnan Province was firstly reported, which was determined through polymerase chain reaction (PCR) method. The total length of mitochondrial genome of Diannan small-ear pig was 16720 bp, including 34.77% A, 26.18% C, 25.81% T and 13.24% G, and in the order A > C > T > G. Mitochondrial genome contained a major non-coding control region (D-Loop region), 2 ribosomal RNA genes, 13 protein-coding genes (PCGs) and 22 transfer RNA genes. The mitochondrial genome of Diannan small-ear pig provides an important data set for the study on genetic mechanism.

  8. The complete sequence of mitochondrial genome of Wuzhishan pig (Sus Scrofa).

    Science.gov (United States)

    Chai, Yu-Lan; Xu, Dong; Ma, Hai-Ming

    2016-01-01

    In the present study, we sequenced the complete mitochondrial genome of Wuzhishan pig, which was 16,741 bp in size and had a nucleotide composition in A and T (60.46%). The genome consisted of a major non-coding control region (D-loop region) and 37 genes, including 2 ribosomal RNA (rRNA) genes, 13 protein-coding genes (PCGs), and 22 transfer RNA (tRNA) genes. The genes in the mitochondrial genomes of Wuzhishan pig used three kinds of initiation codons (ATA, ATG, and GTG) and four kinds of termination codons (TAA, AGA, TAG, and an incomplete termination codons T-). The complete mitochondrial genome sequence of Wuzhishan pig provides an important data set for further study on genetic mechanism.

  9. The complete sequence of the mitochondrial genome of Lantang pig (Sus scrofa).

    Science.gov (United States)

    Ran, Mao-Liang; Liu, Zhen; Yang, An-Qi; Li, Zhi; Chen, Bin

    2016-01-01

    Lantang pig is a native breed of Guangzhou Province in China. It is the first time that the complete mitochondrial genome sequence of Lantang pig is reported in this work, which is determined through the PCR-based method. The total length of the mitognome is 16,709 bp, which contains 2 ribosomal RNA genes, 22 tRNA genes, 13 PCGs and 1 conntrol region (D-loop region, Table 1). The total base composition of Lantang pig mitochondrial genome is 34.69% for A, 26.18% for C, 25.82% for T and 13.31% for G, in the order A>C>T>G. The complete mitochondrial genome of Lantang pig provides an important data in genetic mechanism and the evolution genomes.

  10. Spatiotemporal Effects of Supplementary Feeding of Wild Boar (Sus scrofa) on Artificial Ground Nest Depredation.

    Science.gov (United States)

    Oja, Ragne; Zilmer, Karoline; Valdmann, Harri

    2015-01-01

    Supplementary feeding of ungulates, being widely used in game management, may have unwanted consequences. Its role in agricultural damage is well-studied, but few studies have considered the potential for the practice to attract ground nest predators. Our goal was to identify the factors influencing ground nest predation in the vicinity of year-round supplementary feeding sites for wild boar and to characterise their spatiotemporal scope. We conducted two separate artificial ground nest experiments in five different hunting districts in south-eastern Estonia. The quantity of food provided and distance of a nest from the feeding site were the most important factors determining predation risk. Larger quantities of food resulted in higher predation risk, while predation risk responded in a non-linear fashion to distance from the feeding site. Although predation risk eventually decreases if supplementary feeding is ceased for at least four years, recently abandoned feeding sites still pose a high predation risk.

  11. The complete sequence of the mitochondrial genome of Duroc pig (Sus Scrofa).

    Science.gov (United States)

    Wang, Ling-Yu; Chai, Yu-Lan; Ma, Hai-Ming

    2016-01-01

    In this study, the total length of mitochondrial genome of Duroc pig is 16,731 bp, including 34.66% A, 26.27% C, 25.74% T and 13.33% G. Mitochondrial genome contains a major non-coding control region (D-Loop region), 2 ribosomal RNA genes, 13 protein-coding genes (PCGs) and 22 transfer RNA genes. ND2 selects ATT as the initiation codon, and ATA is chose as an initiation codon in ND3 and ND5, the nonstandard start codon is GTG in ND4L and the rest protein common start codon is ATG. The mitochondrial genome of Duroc pig provides an important data in genetic mechanism, which plays an important role in the three-way crossbred pigs.

  12. The complete sequence of the mitochondrial genome of Rongchang pig (Sus Scrofa).

    Science.gov (United States)

    Wang, Ling-Yu; Xu, Dong; Ma, Hai-Ming

    2016-01-01

    Rongchang pig is one of the native breeds in Sichuan province in China. The total length of mitochondrial genome of Rongchang pig is 16,710 bp, including 34.67% A, 26.18% C, 25.82% T and 13.33% G, and in the order A > C > T > G. Mitochondrial genome contains a major non-coding control region (D-Loop region), 2 ribosomal RNA genes, 13 protein-coding genes (PCGs) and 22 transfer RNA genes. This is the first report of the complete mitochondrial genome sequence about Rongchang pig. The mitochondrial genome of Rongchang pig subsequently provides an important information in genetic mechanism and the evolution genomes.

  13. [HELMINTH FAUNA OF WILD BOARS (SUS SCROFA L.1758) IN AZERBAIJAN].

    Science.gov (United States)

    Fataliev, Q H

    2015-01-01

    A total of 41 wild boar specimens, including 19, 10, 10, and 2 specimens from the Lesser-Caucasus, the Greater Caucasus, the Kura-Araks lowland, and Lankaran natural region were studied. On the whole, 16 helminth species were revealed, including 2, 2, 1, and 11 species of trematodes, cestodes, acanthocephalans, and nematodes. The distribution of helminths in landscape-ecological zones of Azerbaijan is analyzed.

  14. The complete sequence of mitochondrial genome of Laiwu Black pig (Sus Scrofa).

    Science.gov (United States)

    Yang, Hu; Xu, Xing-Li; Ma, Hai-Ming

    2016-01-01

    In the present study, the ear tissue of an adult Laiwu Black pig is from the Shandong province of China. The complete mitochondrial genome of Laiwu Black pig was determined by polymerase chain reaction (PCR). The complete mitochondrial genome is 16,710 bp, and it contains 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, a control region (D-loop), with the genome organization and gene order being identical to that of the typical vertebrates.

  15. Long-Term Surveillance of Aujeszky's Disease in the Alpine Wild Boar (Sus scrofa).

    Science.gov (United States)

    Chiari, Mario; Ferrari, Nicola; Bertoletti, Marco; Avisani, Dominga; Cerioli, Monica; Zanoni, Mariagrazia; Alborali, Loris G; Lanfranchi, Paolo; Lelli, Davide; Martin, Ana Moreno; Antonio, Lavazza

    2015-12-01

    Although wild boar can act as a persistent Aujeszky's disease (AD) reservoir, limited data are available on long-term epidemiology in free-ranging wild boar living in areas where industrial swine herds are limited. Hence, this study provides crucial information, which fills this knowledge gap, on the natural dynamics of AD infection. From 3260 sera sampled during eight hunting seasons, 162 (4.97%) were tested positive. Factors, including the animal's age class, and the sampling year, had significant effects on the probability of the wild boar being seropositive, while wild boar mean abundance per area, yearly abundance and the total number of pig farms, as well as interactions among age, year and sex, were not significant. In particular, a positive trend of seroprevalence was observed over the years, with values ranging from 2.1 to 10.8%. This long-term surveillance showed an increase in seroprevalence with a higher probability of being seropositive in older individuals and the independence of wild boar seropositivity from the likelihood of contact with pigs in the area.

  16. Developmental Stage-Specific Imprinting of IPL in Domestic Pigs (Sus scrofa

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    Shengping Hou

    2010-01-01

    Full Text Available Imprinted in placenta and liver (IPL gene has been identified as an imprinted gene in the mouse and human. Its sequence and imprinting status, however, have not been determined in the domestic pigs. In the present study, a 259 base pair-specific sequence for IPL gene of the domestic pig was obtained and a novel SNP, a T/C transition, was identified in IPL exon 1. The C allele of this polymorphism was found to be the predominant allele in Landrace,Yorkshire, and Duroc. The frequency of CC genotype and C allele are different in Duroc as compared with Yorkshire (P=.038 and P=.005, resp.. Variable imprinting status of this gene was observed in different developmental stages. For example, it is imprinted in 1-dayold newborns (expressed from the maternal allele, but imprinting was lost in 180-day-old adult (expressed from both parental alleles. Real-time PCR analysis showed the porcine IPL gene is expressed in all tested eight organ/tissues. The expression level was significantly higher in spleen, duodenum, lung, and bladder of 180-day-old Lantang adult compared to that in 1-day-old newborns Lantang pigs (P<.05. In conclusion, the imprinting of the porcine IPL gene is developmental stage and tissue specific.

  17. Occurrence of Ochratoxin A in the Wild Boar (Sus scrofa: Chemical and Histological Analysis

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    Elvira De Giglio

    2012-12-01

    Full Text Available Ochratoxins are fungal secondary metabolites that may contaminate a broad variety of foodstuffs, such as grains, vegetables, coffee, dried fruits, beer, wine and meats. Ochratoxins are nephrotoxins, carcinogens, teratogens and immunotoxins in rats and are also likely to be in humans. In 2009/2010, a survey of the presence of Ochratoxin A (OTA in regularly hunted wild boars in the Calabria region of southern Italy detected OTA in 23 animals in the kidney, urinary bladder, liver and muscles: 1.1 ± 1.15, 0.6 ± 0.58, 0.5 ± 0.54 and 0.3 ± 0.26 μg/kg, respectively. Twelve tissue samples showed levels of OTA higher than the guideline level (1 μg/kg established by the Italian Ministry of Health. In five wild boars, gross-microscopic lesions were described for the organs displaying the highest concentrations of OTA determined by HPLC-FLD analysis, i.e., the kidney, liver and urinary bladder.

  18. Sus scrofa: Population Structure, Reproduction and Condition in Tropical North Eastern Australia

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    NELSON, John E.

    2009-01-01

    Full Text Available Three feral pig populations inhabiting contrasting environments along the north easterncoast of Australia have been investigated with respect to population structure, individual condition andreproduction. The population on Prince of Wales Island contains a large proportion of juvenile andsub-adult pigs but lacks pigs in the higher age classes. Individuals also breed at an earlier age thananimals of the mainland populations. Pig populations on Cape York Peninsula show a largerproportion of older animals and feral pigs living in rainforest habitats show a low proportion ofanimals in very young and very old age classes. Pigs from the lowland rainforest population are inbetter condition than those of the other populations for most of the year, reflecting the availability offood all year round in this environment. Differences in the population structure of the threepopulations are discussed with respect to fecundity and several mortality factors such as predation anddiseases/parasites.

  19. Hybridization levels in European Sus scrofa, comparison between genetic and survey data

    DEFF Research Database (Denmark)

    Iacolina, Laura; Bakan, Jana; Cubric-Curik, Vlatka

    2016-01-01

    separation between WB and DP, with a limited number of hybrids in both populations. The introgression level varies considerably among populations, from non-detectable to very high. Perceived presence of hybrids, based on phenotypic characteristics or historic data, is usually higher and widespread than...

  20. Ethnic -Zootechnic characterization and meat potential of Sus scrofa “creole Pig” in Latin America.

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    Virginia Linares

    2011-06-01

    Full Text Available The objective of this systematic scientific review was updated bibliographic information concerning the genetic, morphological, zootechnical and meat potential of creole pigs in Central and South America. It was found a population of 73 million Creoles pigs in Latin America, most under extensive, and semi extensive production systems. Since its European origin, adaptation to different ecosystems on the continent and the introduction of new breeds has led into a wide variety of creole pigs. Adaptation and introduction of breeds have led also in a rise of genetic variability of pigs benefiting the rusticity that involves a more efficient immune system. In the same way as omnivore the creole pig had a good adaptation to different diets, getting better advantage of fibrous and fatty food than commercial breeds. On the other hand, creole pigs showed an acceptable reproductive performance, distinguished by sexual precocity and high feasibility of weaning, nevertheless its growth was slow and lactation periods were long. Regarding to the quality of meat cuts , it has not been disadvantaged, if we take into account subjective criteria as well as tenderness and the nutritional value, characteristics that give higher economic value outstripping commercial pig meat.

  1. Growth pattern of the maxillary sinus in the miniature pig (Sus scrofa).

    Science.gov (United States)

    Koppe, T; Klauke, T; Lee, S H; Schumacher, G

    2000-01-01

    The biological role of the paranasal sinuses is obscure, can be elucidated through a cross-sectional growth study of the maxillary sinus in miniature pigs. The maxillary sinus area was obtained from lateral cephalograms of left skull halves of 103 female miniature pigs of known ages, from newborn to 24 months. Out of several nonlinear models, the growth of the maxillary sinus was best described with the Gompertz model. The first derivative of the Gompertz curve revealed an increase in the growth rates of the maxillary sinus until 4 months, after which sinus growth slowed down. The eruption of the permanent molars did not seem to have a significant influence on this growth pattern. Furthermore, growth in maxillary sinus size in the miniature pig does not follow growth in skull size closely, which showed the highest growth rates in newborn animals. In addition, a correlation analysis revealed that the relationship between maxillary sinus area and different characteristics of the masticatory apparatus (including linear cranial dimensions, and the dry weight of the masseter and zygomatico-mandibularis muscles) were influenced greatly by skull size. These results suggest that the existence of pneumatic cavities within the mammalian skull is not satisfactorily explained solely by an architectural theory. Epigenetic factors are likely to influence the final shape of the maxillary sinus.

  2. Brain Mass and Encephalization Quotients in the Domestic Industrial Pig (Sus scrofa.

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    Serena Minervini

    Full Text Available In the present study we examined the brain of fetal, newborn, and adult pigs raised for meat production. The fresh and formalin-fixed weights of the brain have been recorded and used, together with body weight, to calculate the Encephalization Quotient (EQ. The weight of the cerebellum has been used to calculate the Cerebellar Quotient (CQ. The results have been discussed together with analogue data obtained in other terrestrial Cetartiodactyla (including the domestic bovine, sheep, goat, and camel, domesticated Carnivora, Proboscidata, and Primates. Our study, based on a relatively large experimental series, corrects former observations present in the literature based on smaller samples, and emphasizes that the domestic pig has a small brain relative to its body size (EQ = 0.38 for adults, possibly due to factors linked to the necessity of meat production and improved body weight. Comparison with other terrestrial Cetartiodactyla indicates a similar trend for all domesticated species.

  3. Meiotic recombination analyses of individual chromosomes in male domestic pigs (Sus scrofa domestica).

    Science.gov (United States)

    Mary, Nicolas; Barasc, Harmonie; Ferchaud, Stéphane; Billon, Yvon; Meslier, Frédéric; Robelin, David; Calgaro, Anne; Loustau-Dudez, Anne-Marie; Bonnet, Nathalie; Yerle, Martine; Acloque, Hervé; Ducos, Alain; Pinton, Alain

    2014-01-01

    For the first time in the domestic pig, meiotic recombination along the 18 porcine autosomes was directly studied by immunolocalization of MLH1 protein. In total, 7,848 synaptonemal complexes from 436 spermatocytes were analyzed, and 13,969 recombination sites were mapped. Individual chromosomes for 113 of the 436 cells (representing 2,034 synaptonemal complexes) were identified by immunostaining and fluorescence in situ hybridization (FISH). The average total length of autosomal synaptonemal complexes per cell was 190.3 µm, with 32.0 recombination sites (crossovers), on average, per cell. The number of crossovers and the lengths of the autosomal synaptonemal complexes showed significant intra- (i.e. between cells) and inter-individual variations. The distributions of recombination sites within each chromosomal category were similar: crossovers in metacentric and submetacentric chromosomes were concentrated in the telomeric regions of the p- and q-arms, whereas two hotspots were located near the centromere and in the telomeric region of acrocentrics. Lack of MLH1 foci was mainly observed in the smaller chromosomes, particularly chromosome 18 (SSC18) and the sex chromosomes. All autosomes displayed positive interference, with a large variability between the chromosomes.

  4. Cooperation improves the access of wild boars (Sus scrofa) to food sources.

    Science.gov (United States)

    Focardi, S; Morimando, F; Capriotti, S; Ahmed, A; Genov, P

    2015-12-01

    Wild boar is a highly polycotous ungulate species, characterized by a complex and dynamical social organization based on the maintenance of long-term bonds between mother and daughters. The roots of this social organization have to be researched at the individual level, considering adaptations that improve fitness in hostile environments. We used information collected by camera-traps at artificial feeding sites, in two contrasting environments in Bulgaria (mountain habitat) and Italy (sub-Mediterranean habitat). We recorded 417 and 885 distinct groups on 7 and 11 foraging sites in Bulgaria and Italy, respectively. We computed (controlling for time range, study area and supplementary feeding site) an index of effective foraging time of the different social groups. We observed a positive and significant effect of the number of conspecifics of the same social group on the effective foraging time. The impact of the other social classes on effective foraging time is also positive, and males, yearlings, and juveniles benefited more from the presence of other social classes, while females were less affected. The access of the different social groups to foraging sites is not random. Males and yearlings play producers (i.e., search for food) and are prone to attend foraging sites before adult females and subadults, so attaining a larger foraging efficiency with respect to a situation where other groups are already present on the feeding site. Wild boars exhibit a more complex social organisation than previously believed, where cooperation prevails largely on competition. A rough division of labour is also present: yearlings, males, and juveniles use to play producers and assume a significant amount of risk determined by the presence of predators or hunters.

  5. Evidence for Chlamydiaceae and Parachlamydiaceae in a wild boar (Sus scrofa population in Italy

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    Antonietta Di Francesco

    2013-03-01

    Full Text Available Conjunctival swabs from 44 free-living wild boars culled during a demographic control programme applied in a Regional Park located in the Northern Italy were examined by 16S rRNA encoding gene nested PCR. In total, 22 (50% wild boars were PCR positive. Sequencing of the amplicons identified Chlamydia suis and Chlamydia pecorum in 12 and 5 samples, respectively. For one sample found PCR positive, the nucleotide sequence could not be determined. Four conjunctival samples showed ≥ 92% sequence similarities to 16S rRNA sequences from Chlamydia-like organisms, as did large intestine, uterus, and vaginal swabs from the same four animals. Amoeba DNA was found in one Chlamydia-like organism positive conjunctival swab. To our knowledge, this is the first detection of members of the Parachlamydiaceae family in wild boars, confirming a large animal host range for Chlamydia-like organisms.

  6. Wild boar (Sus scrofa) - reservoir host of Toxoplasma gondii, Neospora caninum and Anaplasma phagocytophilum in Slovakia.

    Science.gov (United States)

    Reiterová, Katarína; Špilovská, Silvia; Blaňarová, Lucia; Derdáková, Markéta; Čobádiová, Andrea; Hisira, Vladimír

    2016-03-01

    In Central Europe the wild boar population is permanently growing and consequently Cf foodborne infections. In this study serological and molecular detection of Toxoplasma gondii and Neospora caninum in wild boars was evaluated. Moreover, same samples were screened for the presence and genetic variability of tick-borne bacterium Anaplasma phagocytophilum. Blood samples collected from 113 wild boars from Southern Slovakia were examined for antibodies to T. gondii by indirect and to N. caninum by competitive ELISA. The presence of parasitic DNA in blood samples was determined by standard or real time PCR techniques. Antibodies against T. gondii and N. caninum were detected in 45 (39.8%) and 38 (33.6%) animals, respectively. Females were more frequently infected for both pathogens than males. The high seropositivity against both coccidia indicates a permanent occurrence of these pathogens in the studied locality. T. gondii DNA was confirmed in five seropositive boars (4.4%) and N. caninum in 23 blood samples (20.4%). Three out of 23 N. caninum PCR positive animals did not show seropositivity. Three out of 113 blood samples of wild boars were positive for A. phagocytophilum (2.7%). The obtained A. phagocytophilum sequences were 100% identical with GenBankTM isolates from Slovak dog (KC985242); German horse (JF893938) or wild boar (EF143810) and red deer (EF143808) from Poland. Coinfections of T. gondii with N. caninum and N. caninum with A. phagocytophilum were detected in single cases. Results suggest a potential zoonotic risk of toxoplasmosis transmission to humans and the spread of neosporosis to farm animals.

  7. Wild boars (Sus scrofa) as bioindicators of environmental levels of selenium in Poland.

    Science.gov (United States)

    Nowakowska, E; Pilarczyk, B; Pilarczyk, R; Tomza-Marciniak, A; Bąkowska, M; Marciniak, A

    2016-12-01

    The objective of the study was to determine selenium content in selected organs (liver, kidney) of wild boars from different regions of Poland. Materials for the study were obtained from 28 sites located in 16 provinces of Poland. Selenium concentrations in organs were determined using spectrofluorometric methods after wet mineralization in HNO3 and HClO4 mixture. Mean selenium concentrations in the investigated wild boars from Poland were 0.230 μg/g wet weight in the liver and 1.327 μg/g w.w. in the kidneys. Hepatic and nephric Se concentrations ranged from 0.036-0.626 μg/g w.w. and 0.322-4.286 μg/g w.w., respectively. Selenium concentrations in the wild boars differed considerably according to geographical location. Concentrations of selenium were highest in wild boars from south-eastern provinces and lowest in animals from northern provinces. Most of Poland's area is environmentally deficient in this trace element, as evidenced by marginal selenium levels in the organs of the wild boars.

  8. The phenotypic characterization of wild boar population in Transylvania "Sus Scrofa Ferus"

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    Voichita Ana Maria Gavrila

    2016-01-01

    Full Text Available The surveys were conducted over the years 2013-2014. The biological material was represented by 43 females and 63 males, adults, with the age of over 3 years, harvested from three hunting grounds in Transylvania. Conformation measurements were made for the following characteristics: body length, height at the withers, the croup height, thorax perimeter, body weight, head length, forehead width between the ears. There were estimated average and dispersion factors for each characteristics and phenotypic correlations were estimated between concerned characteristics. There is a large variability in all studied characteristics, in both males and females, variability given by individual differences and higher performance limits for each characteristics, given by both individual variability and environmental condition and harvesting season.

  9. Prevalence and antimicrobial susceptibility of salmonella in European wild boar (Sus scrofa); Latium Region - Italy.

    Science.gov (United States)

    Zottola, T; Montagnaro, S; Magnapera, C; Sasso, S; De Martino, L; Bragagnolo, A; D'Amici, L; Condoleo, R; Pisanelli, G; Iovane, G; Pagnini, U

    2013-03-01

    The prevalence of Salmonella spp. infection was determined in 499 wild boars harvested during the 2010-2011 and 2011-2012 hunting seasons in the Latium Region of Italy. We conducted a microbiological assessment on faeces collected at slaughter and we examined serum samples for the presence of antibodies to Salmonella spp. by ELISA assay. Out of 383 serum samples examined, 255 (66.5%) were positive for Salmonella spp. antibodies. Overall, 10.8% (54/499) of the animals were positive by microbiological assessment. The Salmonellae most frequently isolated were S. enterica subsp. salamae II (24%), S. enterica subsp. Diarizonae III b (12.9%), S. enterica subsp. houtenae IV (11.1%) and S. Fischerhuette (7.4%); less common Salmonella isolates included S. Veneziana (5.5%), S. Napoli (5.5%), S. Kottbus (5.5%), S. Thompson (5.5%), S. enterica subsp. arizonae III a (3.7%), S. Toulon (3.7%), S. Burgas (1.8%), S. Tennelhone (1.8%), S. Ferruch (1.8%), S. choleraesuis (1.8%), S. Paratyphi (1.8%), S. Stanleyville (1.8%), S. Typhimurium (1.8%) and S. enterica subsp. enterica 4,5,12:1:- (1.8%). These isolates were tested against 16 antimicrobial agents and exhibited resistance to sulphonamides (92.5%), sulphonamides and thrimetroprim (14.8%), colistin (14.8%), streptomycin (18.5%), gentamycin (5.5%), tetracycline (5.5%), ceftiofur (3.7%), cefazoline (1.8%), cefotaxime (1.8%), nalidixic acid (1.8%), amoxicillin and clavulanic acid (1.8%) and ampicillin (3.7%). Our data, the first collected on this species in Italy, suggest that European wild boars are frequent carriers of antimicrobial-resistant Salmonellae and are likely involved in the transmission of antimicrobial resistance throughout the environment.

  10. Evidence of low prevalence of mycobacterial lymphadenitis in wild boars (Sus scrofa) in Poland.

    Science.gov (United States)

    Witkowski, Lucjan; Orłowska, Blanka; Rzewuska, Magdalena; Czopowicz, Michał; Welz, Mirosław; Anusz, Krzysztof; Kita, Jerzy

    2017-01-25

    Mycobacterium spp. and Rhodococcus equi are generally regarded as the main causes of lymphadenitis in pigs and wild boars. In Poland, mycobacterial submandibular lymphadenitis was first diagnosed in a wild boar in 2012 but Mycobacterium spp. infections are also present in the Polish population of European bison (Bison bonasus). The prevalence of lymphadenitis in Polish wild boars has been found to 8.4% (95% CI 6.2-11.3%) and it has been proved that R. equi is not an important cause of purulent lesions in these animals. The current study was carried out to assess the prevalence of mycobacterial lymphadenitis in the Polish wild boar population. Submandibular lymph nodes with purulent lesions collected from 38 wild boars in 2010/2011 and negative for R. equi were included. Calculations based on the hypergeometric approximation were used to determine the probability that at least one positive individual would be detected if the infection had been present at a prevalence greater than or equal to the design prevalence. All 38 samples were negative for Mycobacterium spp. [0% (95% CI 0, 9.2%)]. Epidemiological analysis showed that the true prevalence was 95% likely to be lower than 10%. In conclusion, mycobacterial lymphadenitis seems to occur rarely in wild boars in Poland. Due to the presence of Mycobacterium spp. infections in other wildlife, the surveillance of mycobacterial infections in wild animals in Poland remains an important issue.

  11. Effects of acute dietary iron overload in pigs (Sus scrofa) with induced type 2 diabetes mellitus.

    Science.gov (United States)

    Espinoza, A; Morales, S; Arredondo, M

    2014-06-01

    Epidemiological studies have reported an association between high iron (Fe) levels and elevated risk of developing type 2 diabetes mellitus (T2D). It is believed that the formation of Fe-catalyzed hydroxyl radicals may contribute to the development of diabetes. Our goal was to determine the effect of a diet with a high Fe content on type 2 diabetic pigs. Four groups of piglets were studied: (1) control group, basal diet; (2) Fe group, basal diet with 3,000 ppm ferrous sulfate; (3) diabetic group (streptozotocin-induced type 2 diabetes) with basal diet; (4) diabetic/Fe group, diabetic animals/3,000 ppm ferrous sulfate. For 2 months, biochemical and hematological parameters were evaluated. Tissue samples of liver and duodenum were obtained to determine mRNA relative abundance of DMT1, ferroportin (Fpn), ferritin (Fn), hepcidin (Hpc), and transferrin receptor by qRT-PCR. Fe group presented increased levels of hematological (erythrocytes, hematocrit, and hemoglobin) and iron parameters. Diabetic/Fe group showed similar behavior as Fe group but in lesser extent. The relative abundance of different genes in the four study groups yielded a different expression pattern. DMT1 showed a lower expression in the two iron groups compared with control and diabetic animals, and Hpc showed an increased on its expression in Fe and diabetic/Fe groups. Diabetic/Fe group presents greater expression of Fn and Fpn. These results suggest that there is an interaction between Fe nutrition, inflammation, and oxidative stress in the diabetes development.

  12. [Basic values of blood coagulation parameters in pigs (Sus scrofa domesticus)].

    Science.gov (United States)

    Hahn, N; Popov-Cenic, S; Dorer, A

    1996-01-01

    On 23 clinical healthy pigs (2-4 months of age, body weight 13-42 kg) under ketamin-pentobarbital anaesthesia blood plasma coagulation parameters have been investigated. To obtain basic values 26 parameters were measured: number of thrombocytes, parameters of thrombelastogram and resonance-thrombogram, prothrombin time, activated partial thromboplastin time, thrombin time, reptilase time, factors I, II, V, VII, VIII, X, antithrombin III, plasminogen, alpha 1-antitrypsin, alpha 2-antiplasmin, alpha 2-macroglobulin, fibrin degradation products D and E and euglobulin lysis-time. Parameters calculated in percent should be measured against a pig plasma pool. Measurement against a human plasma pool are hardly valid in values higher than 100%. In comparison to man the results indicate modifications of fibrinogenesis and fibrinolysis in pigs.

  13. Mycobacterium bovis infection in a wild sow (Sus scrofa): first case in Korea.

    Science.gov (United States)

    Ku, Bok Kyung; Jeon, Bo-Young; Kim, Jae Myung; Jang, Young-Boo; Jang, Yunho; Yu, So Yoon; Kim, Jiro; Moon, Oun Kyung; Jung, Suk Chan; Lee, Min Kwon; Jeong, Tae Nam

    2016-09-30

    Mycobacterium (M.) bovis causes tuberculosis and has a broad host range, including humans, livestock, and wild animals. M. bovis infection of wild boar has been reported in several European countries. We report here the first case of M. bovis infection in a domesticated wild sow in Korea. Granulomatous and necrotizing lesions with small numbers of acid-fast bacilli were observed in nodules of the lung of wild sow. Furthermore, the M. bovis isolate from the wild sow had spoligotype SB0140 and a novel MIRU-VNTR allelic profile, which is not found in cattle and deer in Korea.

  14. Dietary fiber stabilizes blood glucose and insulin levels and reduces physical activity in sows (Sus scrofa)

    NARCIS (Netherlands)

    Leeuw, de J.A.; Jongbloed, A.W.; Verstegen, M.W.A.

    2004-01-01

    The aim of this study was to test whether a diet with a high level of fermentable dietary fiber can stabilize interprandial blood glucose and insulin levels, prevent declines below basal levels, and reduce physical activity in limited-fed breeding sows. Stable levels of glucose and insulin may preve

  15. Implications of food patch distribution on social foraging in domestic pigs (Sus scrofa)

    DEFF Research Database (Denmark)

    Thomsen, Liat Romme; Nielsen, Birte Lindstrøm; Larsen, Ole Næsbye

    2010-01-01

    Feeding behaviour of social animals can be influenced by the spatial distribution of resources. In domestic housing facilities growing pigs will often be fed from feeding sites confined to a small area, i.e. effectively a clumping of food resources. In the present experiment we investigated how f...

  16. Induced pluripotent stem cells from swine (Sus scrofa): why they may prove to be important.

    Science.gov (United States)

    Roberts, R Michael; Telugu, Bhanu Prakash V L; Ezashi, Toshihiko

    2009-10-01

    Three recent papers, published almost simultaneously by different groups, have described the generation of induced pluripotent stem (iPS) cells from the pig, a species whose size, anatomy and physiology render them attractive as clinical models for the human. The approach used in each case was to infect somatic cells with integrating retroviral vectors designed to express four reprogramming genes (POU5F1, SOX2, cMYC and KLF4). The cell lines generated met the standard criteria for pluripotency, including the ability to differentiate along multiple tissue lineages. In most respects, the porcine iPS cells more resembled human embryonic stem cells and human iPS cells than their murine equivalents. Provided such porcine iPS cells can be "personalized" to specific pigs and then coaxed to differentiate along specific lineages, it should be possible to use such animals to test transplantation therapies with iPS cells for safety and efficacy before the procedures are applied to human patients.

  17. ABO and RH1 blood group phenotyping in pigs (Sus scrofa) using microtyping cards.

    Science.gov (United States)

    Martínez-Alarcón, L; Ramis, G; Majado, M J; Quereda, J J; Herrero-Medrano, J M; Ríos, A; Ramírez, P; Muñoz, A

    2010-01-01

    Transplantation or transfusion with ABO disparity is a cause for rejection or for severe hemodynamic alterations. ABO groups in pigs are commonly an unknown variable, which has been previously assessed by means of hemagglutination tests or immunohistochemical procedures on tissues. Herein, we have reported a simple method using commercial microcards for human ABO typing. However, the reagents directly derived from human sera included in these cards can result in false determinations due to alpha-gal interference. The ABO groups of 19 wild-type pigs (Landrace x Large White) were assessed using 2 commercial cards: Human sera-based and monoclonal antibody-based cards. The human sera cards determined that 8 pigs belonged to the AB group and 11 to the B group. The monoclonal antibody cards determined that 8 pigs belonged to the A group and 11 to the O group. None of the pigs showed reactions to Rh1 antibodies. Because the B group has not been described in pigs, the reaction in human sera cards represented an interference with alpha-gal antigen, a molecule structurally similar to the B blood antigen. Thus, microtyping cards based on monoclonal antibodies provided simple, quick way to assess ABO groups in pigs used for xenotransplantation. ABO concordance should always be investigated for these types of procedures.

  18. Mapping carcass and meat quality QTL on Sus Scrofa chromosome 2 in commercial finishing pigs

    Directory of Open Access Journals (Sweden)

    van Kampen Tony A

    2009-01-01

    Full Text Available Abstract Quantitative trait loci (QTL affecting carcass and meat quality located on SSC2 were identified using variance component methods. A large number of traits involved in meat and carcass quality was detected in a commercial crossbred population: 1855 pigs sired by 17 boars from a synthetic line, which where homozygous (A/A for IGF2. Using combined linkage and linkage disequilibrium mapping (LDLA, several QTL significantly affecting loin muscle mass, ham weight and ham muscles (outer ham and knuckle ham and meat quality traits, such as Minolta-L* and -b*, ultimate pH and Japanese colour score were detected. These results agreed well with previous QTL-studies involving SSC2. Since our study is carried out on crossbreds, different QTL may be segregating in the parental lines. To address this question, we compared models with a single QTL-variance component with models allowing for separate sire and dam QTL-variance components. The same QTL were identified using a single QTL variance component model compared to a model allowing for separate variances with minor differences with respect to QTL location. However, the variance component method made it possible to detect QTL segregating in the paternal line (e.g. HAMB, the maternal lines (e.g. Ham or in both (e.g. pHu. Combining association and linkage information among haplotypes improved slightly the significance of the QTL compared to an analysis using linkage information only.

  19. Scatter hoarding and cache pilferage by superior competitors: an experiment with wild boar (Sus scrofa)

    NARCIS (Netherlands)

    Suselbeek, L.; Adamczyk, V.M.A.P.; Bongers, F.; Nolet, B.A.; Prins, H.H.T.; van Wieren, S.E.; Jansen, P.A.

    2014-01-01

    Food-hoarding patterns range between larder hoarding (a few large caches) and scatter hoarding (many small caches), and are, in essence, the outcome of a hoard size–number trade-off in pilferage risk. Animals that scatter hoard are believed to do so, despite higher costs, to reduce loss of cached fo

  20. Genetic Resources, Genome Mapping and Evolutionary Genomics of the Pig (Sus scrofa)

    OpenAIRE

    Chen, K.; Baxter, T.; Muir, W. M.; Groenen, M.A.M.; Schook, L B

    2007-01-01

    The pig, a representative of the artiodactyla clade, is one of the first animals domesticated, and has become an important agriculture animal as one of the major human nutritional sources of animal based protein. The pig is also a valuable biomedical model organism for human health. The pig's importance to human health and nutrition is reflected in the decision to sequence its genome (3X). As an animal species with its wild ancestors present in the world, the pig provides a unique opportunity...

  1. Single nucleotide markers of D-loop for identification of Indian wild pig (Sus scrofa cristatus)

    OpenAIRE

    Gaurav Kumar Srivastava; Nidhi Rajput; Kajal Kumar Jadav; Avadh Bihari Shrivastav; Himanshu R. Joshi

    2015-01-01

    Aim: Partial fragment of D-loop region extending from 35 to 770 were compared with corresponding sequences of 16 wild pigs and 9 domestic pig breeds from different parts of the world for detection of single nucleotide polymorphism (SNP) markers in the region. The paper also reappraises SNP markers from two fragments of cytochrome b gene and a fragment 12S rRNA gene distinguishing the Indian wild pig from other pig species of the world. Materials and Methods: Deoxyribonucleic acid (DNA) was is...

  2. The establishment and distribution of feral wild boar (Sus scrofa in England

    Directory of Open Access Journals (Sweden)

    C. Wilson

    2014-03-01

    Full Text Available Until recently, geographic isolation has protected Britain from the widespread increases in wild boar populations seen elsewhere in Europe, but following the development of wild boar farming in the 1980s a number of escapes and releases have occurred, resulting in the re-establishment of the species in the wild in England. The present study monitored the establishment and presence of wild boar in England by collating reports of escapes or releases and ground-truthing evidence of animals in the wild. This data is used to give an up-to-date indication of the distribution of the species in England. In the twenty years from 1989/90 to 2008/9 an average of one to two escape/release incidents occurred each year, with individual incidents involving from one to more than 50 individuals. These have resulted in the establishment of at least four distinct populations, the largest of which probably has a pre-breeding population in excess of 200 animals. None of the escapes or releases involving five or fewer individuals is believed to have led to establishment of a population. Based on the availability of woodland, there is potential for a total population in England of around 30,000 – 40,000 animals. However, future development of local populations is likely to be constrained over much of the country because of low woodland cover and culling pressure, and it is likely to take many years for a population of this size to develop, if at all.

  3. Genetic Structure of the Wild Boar (Sus scrofa L. Population in Portugal

    Directory of Open Access Journals (Sweden)

    Fonseca, C.

    2006-06-01

    Full Text Available The main objective of this study was the assessment of the genetic structure and level of variability in the Portuguese wild boar population. A total of 65 wild boar blood samples were collected all over the continental territory, during 2002/03 and 2003/04 hunting seasons. A set of six microsatellite markers, developed for domestic pig, was used. Loci SW986 and SW828 presented a small number of alleles for the Portuguese population, whereas other l o c i, like SW1701 and SW1517, presented a high degree of polymorphism. From the six analysed l o c i, four presented significant deviation from Hardy-We i n b e rg equilibrium conditions, suggesting the existence of genetic structure in the population. Samples were divided into North, Centre and South groups according to the position of wild boar capture location in relation to rivers Douro and Tejo. All the FST estimates were statistically significant and the highest FST value was 0.08 (P<0.001, referring to the distance between Northern and Central groups. FCA analysis was also performed. The resulting bi-dimensional diagram suggests structuring of the Portuguese wild boar population.

  4. Genetic resources, genome mapping and evolutionary genomics of the pig (Sus scrofa)

    NARCIS (Netherlands)

    Chen, K.; Baxter, T.; Muir, W.M.; Groenen, M.A.M.; Schook, L.B.

    2007-01-01

    The pig, a representative of the artiodactyla clade, is one of the first animals domesticated, and has become an important agriculture animal as one of the major human nutritional sources of animal based protein. The pig is also a valuable biomedical model organism for human health. The pig's import

  5. Occurrence of Ochratoxin A in the wild boar (Sus scrofa): chemical and histological analysis.

    Science.gov (United States)

    Bozzo, Giancarlo; Ceci, Edmondo; Bonerba, Elisabetta; Di Pinto, Angela; Tantillo, Giuseppina; De Giglio, Elvira

    2012-12-04

    Ochratoxins are fungal secondary metabolites that may contaminate a broad variety of foodstuffs, such as grains, vegetables, coffee, dried fruits, beer, wine and meats. Ochratoxins are nephrotoxins, carcinogens, teratogens and immunotoxins in rats and are also likely to be in humans. In 2009/2010, a survey of the presence of Ochratoxin A (OTA) in regularly hunted wild boars in the Calabria region of southern Italy detected OTA in 23 animals in the kidney, urinary bladder, liver and muscles: 1.1 ± 1.15, 0.6 ± 0.58, 0.5 ± 0.54 and 0.3 ± 0.26 μg/kg, respectively. Twelve tissue samples showed levels of OTA higher than the guideline level (1 μg/kg) established by the Italian Ministry of Health. In five wild boars, gross-microscopic lesions were described for the organs displaying the highest concentrations of OTA determined by HPLC-FLD analysis, i.e., the kidney, liver and urinary bladder.

  6. DESARROLLO DE UN PROGRAMA PARA GARANTIZAR LA INOCUIDAD EN CARNES DE AVES Y CERDO, RESPECTO A DIOXINAS Y FURANOS

    OpenAIRE

    VALDOVINOS JELDES, CARLOS

    2007-01-01

    El presente proyecto respondió a la necesidad país de contar con un sistema para garantizar la inocuidad de carnes de aves y cerdos respeto de dioxinas y furanos e identificar factores de riesgo que pueden favorecer la contaminación de dichas carnes. Para lo cual se aplico un sistema de muestreo que abarcó todo el ciclo productivo) (nacimiento, crianza y beneficio) de los animales (pavos, pollos y cerdos) con el fin de desarrollar un programa de mejoramiento y control de la inocuidad...

  7. Hygiene and restraint of pigs is associated with absence of Taenia solium cysticercosis in a rural community of Mexico La higiene y el confinamiento de cerdos están asociados con la ausencia de cisticercosis por Taenia solium en una comunidad rural de México

    Directory of Open Access Journals (Sweden)

    Sonia Vázquez-Flores

    2001-12-01

    Full Text Available Objective. To determine the prevalence and risk factors associated to pig cysticercosis in a rural community of Veracruz, Mexico. Material and Methods. Swine cysticercosis was diagnosed by tongue palpation and circulating antibodies in pigs kept in 178 household backyards. Risk factors were assessed by interviewing owners to collect information on pig breeding conditions and demographic characteristics. Results. None of the 53 pigs studied showed cysts in the tongue, nor antibodies against Taenia solium in Western blot assays. Latrines were available in 91% of the houses and pigs were kept in restrained areas. Conclusions. The present study shows that pig breeding under restraint with basic hygiene and sanitary conditions, may be effective and practical interventions to restrain Taenia solium in rural communities. The English version of this paper is available too at: http://www.insp.mx/salud/index.html Objetivo. Determinar la prevalencia y factores de riesgo asociados con cisticercosis porcina en una población rural de Veracruz, México. Material y métodos. Se diagnosticó cisticercosis porcina por medio de palpación lingual y anticuerpos circulantes en cerdos de traspatio en 178 casas. Se analizaron los factores de riesgo mediante una encuesta a los dueños respecto a las condiciones de crianza de los cerdos y sus características demográficas. Resultados. Los 53 cerdos estudiados fueron negativos al metacéstodo de Taenia solium por palpación lingual y para la presencia de anticuerpos contra este agente por inmunoelectrotransferencia. El 91% de las casas contaban con letrinas y los cerdos estaban confinados en zonas restringidas. Conclusiones. Este estudio muestra que el confinamiento de cerdos puede explicar la ausencia de Taenia solium en comunidades rurales, y sugiere que es factible y práctico establecer medidas de intervención. El texto completo en inglés de este artículo también está disponible en: http://www.insp.mx/salud/index.html

  8. Dietas bajas en proteína adicionadas con ácido linoleico conjugado o aceites de soya acidulado para cerdos en engorda

    OpenAIRE

    Martínez Aispuro, Manuel

    2012-01-01

    Reducir la concentración de proteína en las dietas de cerdos adicionando aminoácidos sintéticos mantiene la respuesta productiva, pero la carne tiene un mayor contenido de grasa. El uso de distinto aceite en la dieta de cerdos puede modificar este aspecto; además, de cambiar el contenido de ácidos grasos en la carne por aumento en el consumo de ácidos grasos que no son sintetizados por los cerdos. Se realizaron tres experimentos para evaluar dietas bajas en proteína y dist...

  9. Pérdida de aminoácidos endógenos en cerdos con niveles variables de consumo de alimento

    OpenAIRE

    Miguel Cervantes Ramírez; Víctor González Vizcarra; Salvador Rodríguez Rubí; Gary Cromwell

    2000-01-01

    La pérdida o flujo de aminoácidos endógenos recolectados al final del intestino delgado de cerdos puede ser modificada por la cantidad diaria de alimento consumido. Este estudio se hizo para evaluar el efecto del nivel de consumo de alimento en la pérdida de aminoácidos endógenos por cerdos consumiendo una dieta purificada. Se usaron cuatro cerdos adaptados con una cánula en duodeno proximal y otra en íleon terminal. Se aplicaron cuatro periodos experimentales y en cada uno se evaluó de maner...

  10. Comparison of α-amylase isoforms from the midgut of Cerambyx cerdo L. (Coleoptera: Cerambycidae larvae developed in the wild and on an artificial diet

    Directory of Open Access Journals (Sweden)

    Dojnov Biljana

    2010-01-01

    Full Text Available α-Amylase isoforms of Cerambyx cerdo larvae from the wild (ML and SL and reared in the laboratory (ADL were compared. Three amylase isoforms were presented in the SL and ML extracts while two isoforms were presented in the ADL according to zymogram after isoelectric focusing (IEF. All C. cerdo amylase isoforms were acidic proteins (pI < 3.5. Seven amylase isoforms (ACC 1-7 from the midgut of C. cerdo larvae were found in the ML midgut extract, six in the SL extract, and four in the ADL extract according to native PAGE zymogram. The ADL amylase had the highest activity. All crude midgut extracts of C. cerdo larvae were fractionated on a Superose 12 HR column. The molecular mass of the ACC was estimated to be 34 kDa. .

  11. Paul Schreber, sus psiquiatras y sus manicomios.

    Directory of Open Access Journals (Sweden)

    José María Álvarez Martínez

    1999-01-01

    Full Text Available El magistrado Dr. Paul Schreber (1842-1911 estuvo gravemente trastornado al menos tres veces a lo largo de su vida. En todas sus crisis recibió atención médica; también en todas ellas precisó ser ingresado sin oponer a tal recomendación, en principio, la menor resistencia. Vivió y padeció pues en sus propias carnes tanto las drogas sedantes más empleadas en aquellos años (entre otras: yoduro de potasio, morfina, hidrato de cloral, bromuro, sulfonal e hidrato de amilo, como las habitaciones y celdas destinadas a este tipo de pacientes en clínicas neurológicas y en manicomios estatales; aulló, vociferó y vagó por sus pasillos y patios, forcejeó con los enfermos y respiró la sórdida y ruidosa atmósfera de los asilos de hace cien años, a pesar incluso de ciertos privilegios que su posición y dinero le permitieron.

  12. Jacinto de agua (Eichhornia crassipes una alternativa para la alimentación de cerdos en ceba

    Directory of Open Access Journals (Sweden)

    |MsC. Isaías Febrero Toussaint .- Centro Universitario Las Tuna

    2005-05-01

    Full Text Available Con el objetivo de evaluar el comportamiento productivo de cerdos en ceba alimentados con Jacinto de agua fresco o ensilado (con un 20% de afrecho de trigo, se utilizaron 21 cerdos Yorkshire - Landrace x Duroc de 23 kg de peso vivo promedio y 160 días de edad, distribuidos según diseño completamente aleatorizado en tres tratamientos, los que incluyeron King grass, Jacinto fresco y ensilaje. Estos alimentos se ofrecieron a voluntad y los animales se suplementaron en todos los casos con afrecho de trigo, harina de soya y miel final. Los cerdos fueron alojados en 3 corrales de 7 animales cada uno. La prueba tuvo una duración de 89 días de los cuales los 13 primero fueron de adaptación. Los cerdos fueron pesados a los 13; 24; 45; 59; 74 y 89 días en horas de la mañana antes de ser alimentados. También se determinó la ganancia media total, la ganancia media diaria, el consumo y la conversión alimentaria. No se encontraron diferencias significativas para la ganancia media diaria entre tratamientos a los 89 días de prueba. Los valores más altos fueron de 483,55 y 422,15 g para el Jacinto fresco y el ensilaje de Jacinto respectivamente. El consumo de alimento en base seca fue menor en el grupo que se alimentó con Jacinto fresco (1,01 kg respecto del ensilaje de Jacinto (1,22 kg. Es factible desde el punto de vista económico el uso de estas fuentes en la alimentación de cerdos en ceba, pues el costo de alimentación fue de 21,91; 16,74; y 17,59 $ el costo por peso fue de 0,16; 0,11; y 0,13 $ para el ensilaje de Jacinto, Jacinto fresco y King grass respectivamente. Se sugiere incluir en la dieta de cerdos en ceba el Jacinto de agua lo mismo fresco que ensilado.

  13. Microbiota de jamones de cerdo cocidos asociada al deterioro por abombamiento del empaque

    Directory of Open Access Journals (Sweden)

    Juliana Ossa

    2010-08-01

    Full Text Available Objetivo. Caracterizar la diversidad microbiana y calidad microbiológica e higiénico sanitaria de diferentes marcas de jamones de cerdo cocidos abombados y no abombados comprados en varios supermercados de Bogotá D.C. Materiales y métodos. Se analizaron 10 marcas diferentes de jamones de cerdo cocidos comprados en tres supermercados, refrigerados bajos las mismas condiciones del consumidor, durante 45 días. Se realizó los recuentos en placa de microorganismos de interés en la industria de alimentos y de inocuidad en las muestras con o sin distensión del empaque e identificación por pruebas bioquímicas y PCR para determinar la diversidad de la microbiota. Se aislaron cepas productoras de biopelículas provenientes de la superficie de una planta de alimentos de una de las marcas de los jamones investigadas. Resultados. Se identificaron un total de 139 cepas aisladas del producto terminado, de las cuales un 99% (137 cepas pertenecen al grupo de Bacterias Acido Lácticas (BAL, el 1% restante son levaduras. De las 31 cepas aisladas de las superficies en la zona de tajado, el 97% (30 cepas presentaron formación de biopelículas. Se determinó ausencia de patógenos tanto en el producto terminado como en las muestras de las superficies.Conclusiones. El deterioro causado por abombamiento del empaque fue asociado a la presencia de BAL, debido a que fueron encontradas en la zona de tajado y en el producto terminado, de este último únicamente se aislaron BAL, en mayor proporción cepas correspondientes al género de Lactobacillus sp.

  14. Lesiones en órganos de cerdos posdestete, inducidas por el lipopolisacárido de E. coli

    OpenAIRE

    2013-01-01

    RESUMENObjetivo. Evaluar el efecto de la ingestión de varios niveles de Lipopolisacárido (LPS) de E. coli sobre las manifestaciones clínicas y lesiones en órganos de cerdos recién destetados. Materiales y métodos. El trabajo de campo se realizó en el Centro San Pablo, perteneciente a la Universidad Nacional de Colombia. El estudio se realizó con 52 cerdos destetados (6.5±0.5 kg) a los 21 días de edad. Los animales fueron alimentados durante 10 días con una dieta basal compuesta de leche y alg...

  15. PCV2-DNA in formalin-fixed and paraffin embedded lymph nodes of wild boar (Sus scrofa ssp. scrofa: one sampling approach for two laboratory techniques

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    Morandi Federico

    2012-03-01

    Full Text Available Abstract Superficial inguinal lymph nodes from 72 wild boars examined in a previous immunohistochemical (IHC study on porcine circovirus type 2 (PCV2 were selected for a PCV2 polymerase chain reaction (PCR analysis. Four of these lymph nodes were PCV2-IHC strongly positive with PMWS histological lesions (outcome 1, 6 weak to mild PCV2-IHC positive without PMWS histological lesions (outcome 2 and 62 PCV2-IHC negative. Considering IHC the gold standard for diagnosis, the aims of the study were to evaluate the suitability of the PCV2-DNA extraction from formalin-fixed and paraffin-embedded (FFPE tissue and the sensitivity and specificity of PCR under two IHC interpretations criteria: (A the sample was considered positive if the result was outcome 1; (B the sample was considered positive if the result was outcome 1 or 2. Under (A criteria, sensitivity and specificity of PCR were 100% and 89.7%, respectively; the Cohen's Kappa coefficient was 0.49. Under (B criteria, sensitivity and specificity of PCR were 80.0% and 95.2%, respectively; the Cohen's Kappa coefficient was 0.72. The high Cohen's Kappa coefficient under the (B interpretative criteria indicates good agreement between the two methods. In conclusion, 1 DNA extracted from FFPE specimens of wild boar is suitable for PCR and further represents a screening test for PCV2/PCVD (PCV2 Diseases investigations in wild boar as well; 2 routine histological sampling can also be useful for PCV2 virological studies in wild boar.

  16. Origins and distributions of the cranial and caudal mesenteric arteries in fetuses of wild boars (Sus scrofa scrofa, Linnaeus – 1758

    Directory of Open Access Journals (Sweden)

    Tharlianne Alici Martins de Souza

    2013-03-01

    Full Text Available The anatomical behavior of the cranial and caudal mesenteric arteries was studied in 21 wild boars fetuses, female and male, in which one injected, through the thoracic aorta, a Neoprene Latex “450” solution, stained with a specific pigment and, then, they were fixed in a 10% formaldehyde solution. The analysis of results allowed one to state that the cranial mesenteric artery originated from the abdominal aorta, caudal to the celiac artery, issuing right and left adrenal branches, pancreatic, and the caudal pancreaticduodenal, jejunal, middle and right colic, and ileo-ceco-colic arteries. The caudal mesenteric artery originated from the abdominal aorta, from its caudal third, issuing the left colic and the cranial rectal arteries. Anastomoses were found between jejunal, left colic and cranial rectal, and left and middle colic arteries. In the three species, the cranial and caudal mesenteric, caudal pancreatic-duodenal, jejunal, right and middle colic, and ileo-cecocolic arteries showed similar vascular patterns, whereas the differences with regard to emission of branches or isolated emergence or emergence in common trunk didn’t change the blood supply.

  17. Detección de una mutación puntual en el gen receptor Ryanodina (Ryr 1 en cerdos criollos colombianos

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    Darwin Y Hernández

    2008-12-01

    Full Text Available El síndrome de estrés porcino (PSS es una enfermedad hereditaria monogénica recesiva relacionada con el gen receptor ryanodina (Ryr1. Utilizando PCR-SSCP y PCR-RFLP se tipificaron genéticamente 14 individuos de cerdos comerciales con el rasgo sindactilia (Casco de Mula-CM, 21 San Pedreños -SP y 100 Zungos- ZN. Las razas CM y SP tuvieron las mismas frecuencias alélicas (F(H = 0.79 y F(h = 0.21, mientras que en los cerdos ZN no se encontró el alelo recesivo (h. La heterocigosidad (He fue de 0.28% para los cerdos CM y 0.23% para los SP. La He para la muestra poblacional fue de 0.066.

  18. Planned Distribution Center for Cerdo Real Meat Products in District IV-Batangas Province, Philippines

    Directory of Open Access Journals (Sweden)

    Aile T. Amorado

    2015-11-01

    Full Text Available This study aimed to determine the optimal location for BUKLOD-UNLAD Multi-purpose Cooperative (BUKLOD’s CERDO Real Meat products distribution centers and to identify the market condition once the optimal location was identified. The said cooperative from Dagatan, a barangay of Taysan, Batangas, Philippines identified the need to create distribution center specifically in District IV of Batangas Province due to the growing demand for their real meat products in the area. Hence to address this need, a study to identify for its optimal location was requested by BUKLOD from the researchers. First, interviews with the management and members of the cooperative were done by the researchers to identify the requirements in establishing a distribution center. In the same way, consultations with the 7 municipalities of District IV were piloted to inform them accordingly about the said project. Moreover the researchers also referred to the Comprehensive Land Used Plan 2012-2022 of each municipality provided by the Provincial Planning and Development Office along with geographical maps gathered from the Provincial Engineers Office to acquire specific data on locations of the involved municipalities. Through various facility planning tools such as Factor Rating Method, Analytical Hierarchy Process and Center of Gravity, the researchers identified Rosario, Batangas as the best location for establishing the distribution center. After a market study was conducted, the researchers further proved the viability of establishing BUKLOD’s CERDO’s products’ distribution center in the area.

  19. Lípidos sanguíneos en cerdos alimentados con pijiguao (Bactris gasipaes Kunth y lisina sintética

    Directory of Open Access Journals (Sweden)

    Janeth Colina R.

    2011-12-01

    Full Text Available Objetivo. Evaluar el efecto de la harina de pijiguao y lisina sintética sobre los lípidos sanguíneos de cerdos en crecimiento y engorde. Materiales y métodos. El estudio se realizó en dos etapas. En la primera etapa se utilizaron 72 cerdos castrados en crecimientode 30 ± 0.5 kg, en un arreglo factorial 2x3: dos niveles de lisina sintética (0 y 2.70 g/kgy tres niveles de harina de pijiguao (0, 160 y 320 g/kg. En la segunda etapa se utilizaron 16 cerdos en engorde de 67.25 ± 1.17 kg, en un arreglo factorial 2x2: dos niveles de lisinasintética (0 y 2.70 g/kg y dos niveles de pijiguao (0 y 175 g/kg. Se determinaron las concentraciones séricas de triacilgliceroles, colesterol total y ácidos grasos. Resultados. Los cerdos en crecimiento que consumieron pijiguao presentaron menores (p<0.001concentraciones de colesterol que el grupo control (2.27 y 2.23 mmol/l vs 2.56 mmol/l y triacilgliceroles (0.34 y 0.28 mmol/l vs 0.42 mmol/l. El ácido oleico incrementó (p<0.01con el mayor nivel de pijiguao (20.78% a 28.84%, y la lisina aumentó (p<0.05 el ácidolinoleico (27.83% a 31.29%. Los cerdos alimentados con pijiguao y lisina mostraron menor (p<0.001 ácido palmítico que el grupo con pijiguao sin lisina (0.23 y 0.19% vs 0.45 y 0.62%, respectivamente. En la etapa de engorde los triacilgliceroles disminuyeron (p<0.05 en los cerdos alimentados con pijiguao y lisina (0.46 a 0.36 mmol/l. Los cerdos alimentados con pijiguao mostraron menor ácido linoleico y mayor ácido oleico (p<0.001. Conclusiones. Las dietas con pijiguao y lisina sintética no causaron efectos negativos sobre los lípidos sanguíneos de cerdos.

  20. Características endocrinas, moleculares y de parámetros de crecimiento asociados a la obesidad del cerdo Pelón Mexicano

    OpenAIRE

    Carmen Camacho-Rea; Carlos G. Gutiérrez; Miguel E. Arechavaleta-Velasco; Laura Díaz-Cueto; Fabián J. Arechavaleta-Velasco; Roberto Martínez Rodríguez; Clemente Lemus-Flores; Rogelio A. Alonso-Morales

    2008-01-01

    Para determinar el potencial del cerdo Pelón Mexicano (CPM) en el estudio de la obesidad, se realizó un estudio entre el CPM y cerdos Landrace-Yorkshire (CLY). Se midieron concentraciones séricas de leptina e insulina y se determinó su correlación con el espesor de grasa dorsal (EGD). Se evaluó el consumo de alimento (CA), el EGD, la ganancia diaria de peso y la expresión de genes que codifican para leptina, el receptor de leptina, adiponectina y el receptor activado por proliferadores de per...

  1. Evaluación de tres niveles de zeolita como promotor natural de crecimiento en dietas en las fases de inicio y acabado de cerdos confinados

    OpenAIRE

    Melendez Vera, Marcela; Rodriguez Alava, Johns

    2009-01-01

    Mediante un diseño de bloques completamente al azar, compuesto por cuatro repeticiones y cuatro tratamientos, se condujo un ensayo con el objetivo de evaluar el efecto de la incorporación de zeolita como promotor de crecimiento sobre el peso corporal, el aumento de peso, consumo de alimento, conversión alimenticia y espesor de grasa dorsal en dietas para cerdos. Se utilizaron 16 cerdos híbridos Landrace x Yorkshire machos castrados y hembras, con un peso promedio de 18 75 kg. Se evaluó ...

  2. Meta-análisis de dietas con baja proteína adicionadas con aminoácidos sintéticos para cerdos en engorda.

    OpenAIRE

    González Reyes, Mónica

    2013-01-01

    Se han reportado resultados inconsistentes utilizando dietas bajas en proteína cruda formuladas con sorgo-pasta de soya adicionadas con AA sintéticos que mantengan los resultados productivos y las características de la canal de cerdos alimentados con dieta estándar. Esto ha motivado a realizar un meta-análisis para combinar los resultados originales de estudios independientes. El conjunto de datos utilizados se basó en una línea de investigación sobre la alimentación de cerdos con dietas baja...

  3. Evaluación del suministro de agua residual tratada por separación-sedimentación-filtración en la salud de cerdos destetados

    OpenAIRE

    Abigail Bravo Alcántara; Aldo Mejía Ramos; Gerardo Ramírez Hernández; Marco Antonio Herradora Lozano; José Luis Pablos Hach; Roberto Martínez Gamba

    2008-01-01

    Los objetivos fueron la cuantificación de enterobacterias (Ent) y la identificación de Salmonella spp en agua residual obtenida a partir de un tratamiento de separación-sedimentación-filtración y evaluar su efecto en la salud de cerdos destetados. En la etapa 1 se obtuvieron dos muestras de la fosa de sedimentación y de tres filtros, por cinco semanas, dando un total de 40 muestras. En la etapa 2 se emplearon 24 cerdos sujetos a 3 tratamientos: (T1= agua filtrada y clorada; T2= agua filtrada;...

  4. Valoración de parámetros clínicos y lesiones en órganos de cerdos durante el período posdestete

    OpenAIRE

    2012-01-01

    El destete de cerdos estã asociado con multiples factores que generan respuestas infiamatorias tem- pranas en órganos internos y alta incidencia de diarreas. En el Centro de Investigación San Pablo de la Universidad Nacional de Colombia sede Medellín, se evaluaron los parámetros clínicos y las lesiones en órganos internos en dieciséis cerdos destetados a veintiún días de edad, que fueron alimentados durante diez días con una dieta a base de leche. Cada uno, cinco, siete y diez días posdestete...

  5. Diversidad genética en cerdos criollos mexicanos con genes candidatos asociados a características productivas

    Directory of Open Access Journals (Sweden)

    Alejandro Antonio González Sarabia

    2011-01-01

    Full Text Available El objetivo de este trabajo fue comparar la variabilidad, diversidad y distancias genéticas entre cerdos criollos, Pelón Mexicano (CPM y Cuinos (CC, con Yorkshire, cuanto a los genes candidatos CAST, DECR1, HAL, HFABP4, LEP, LIPE, MCR4, MYOG, RN y CHX, a través de analysis por PCR-RFLP. Se evaluaron 180 cerdos: 59 CPM, 65 CC y 56 Yorkshire. Se analizaron las frecuencias génicas y genotípicas, heterocigosidad, distancias genéticas y árboles filogenéticos entre grupos raciales. Para CAST, DECR1, HFABP4, LEP, MCR4 y CHX las frecuencias génicas y genotípicas fueron diferentes al comparar las tres razas. En LIPE, los CC fueron iguales a los Yorkshire; en cuanto a MYOG, los CPM fueron iguales a los Yorkshire. No hubo diferencias entre poblaciones criollas y Yorkshire en las frecuencias génicas y genotípicas para HAL y RN. Los cerdos Yorkshire presentaron mayor frecuencia en alelos favorables para CAST, LIPE, MCR4 y MYOG, menor frecuencia de DECR1, HFABP4, CHX, y moderada en LEP. La heterocigosidad promedio para todos los genes fue mayor en CPM (0,42±0,05 y similar en CC (0,33±0,06 y Yorkshire (0,35±0,05. Al calcular distancias genéticas con todos los genes, los CC se encuentran más distantes de los Yorkshire.

  6. Contenidos de isomeros trans de los acidos grasos en productos carnicos (II). Tejidos adiposo y grasa intramuscular del cerdo

    OpenAIRE

    Lluch, M. C.; Pascual, J.; Parcerisa Egea, Javier; Guardiola Ibarz, Francesc; Codony Salcedo, Rafael; Rafecas Martínez, Magdalena; Boatella Riera, Josep

    1993-01-01

    Se presentan los resultados obtenidos en la determinación de ácidos grasos trans en una serie de muestras de tejidos subcutáneo y muscular, procedentes de canales de cerdo, mediante cromatografía en fase gaseosa. Ambos tipos de grasas presentan valores similares y relativamente bajos, con un valor medio del 0.6% de 018:1 t. Se detecta una marcada influencia de la alimentación, puesta de manifiesto por las diferencias significativas obtenidas para los contenidos de C18:1 trans, en función del ...

  7. UTILIZACION AGRONOMICA DE PURINES DE CERDO EN BROCOLI Y SANDIA EN CONDICIONES MEDITERRANEAS SEMIARIDAS. INFLUENCIA EN SISTEMA SUELO-PLANTA

    OpenAIRE

    LLONA CARRASCO, MIRIAM

    2005-01-01

    El hecho de que los purines de cerdo actualmente constituyan un problema medio ambiental grave se debe principalmente al cambio en el sistema de explotación ganadera, ligado a su intensificación. Entre los años 60 y 90, hubo en España una reducción de las explotaciones de porcino con tierra del 75% (Coll, 1993). Esta tendencia a un sistema productivo de tipo Intensivo, con explotaciones sin tierra, alta mecanización, alimentación a base de piensos compuestos, etc., lleva consigo una serie ...

  8. EFECTO DE DIETAS CON ÁCIDOS GRASOS POLIINSATURADOS EN LAS PROPIEDADES SENSORIALES DE LA CARNE DE CERDO

    OpenAIRE

    Jarumi Aguilar-Guggembuhl; Daniel Mota-Rojas; Héctor Escalona-Buendía; M. Elena Trujillo-Ortega; Isabel Guerrero-Legarreta

    2014-01-01

    El consumo de algunos ácidos grasos poliinsaturados de ca - dena larga (AGPCL), en particular los ácidos docosahexa - noico (ADH), eicosapentanoico (AEP) y linoleico conjugado (ALC), es benéfico para la salud humana, por lo cual su in - corporación a la carne de abasto le da un valor agregado, pero las características sensoriales podrían alterarse ocasionando el rechazo del consumidor. El objetivo del presente estudio fue evaluar el efecto de adicionar estos tres AGPCL a la dieta de cerdos en...

  9. Harina o ensilaje de follajes de leguminosas tropicales como fuente de proteína para cerdos

    OpenAIRE

    Sarria Buenaventura, Patricia Isabel

    2013-01-01

    Bajo la hipótesis “Algunos follajes de leguminosas tropicales ensilados o secos, reemplazan por lo menos el 20% de la proteína de la soya en las dietas balanceadas para cerdos en crecimiento sin afectar el rendimiento productivo” se desarrolló la presente investigación. Dos especies fueron evaluadas: Canavalia brasiliensis CIAT 17009, y Vigna unguiculata CIAT 4555. En experimentos separados para cada leguminosa, la harina de follaje de Canavalia reemplazó hasta el 16% y la harina de Vigna has...

  10. Efecto de los tipos de alimentación durante el cebo de cerdos híbridos

    OpenAIRE

    Daza Andrada, Argimiro; García Jiménez, J. M.; Callejo Ramos, Antonio; Ovejero Rubio, Ismael; Buxade Carbo, Carlos

    1990-01-01

    En los últimos años la dinámica productiva del cerdo de abasto ha tendido a la consecución de animales con poca grasa y elevado porcentaje de músculo. La demanda de los consumidores de carnes magras por un lado y la acuciante necesidad de abaratar los costes de producción por otro, han originado que la selección, la alimentación y el manejo se hayan puesto al servicio de tal objetivo.

  11. NIVEL DE PROTEÍNA, FIBRA Y CULTIVO DE LEVADURA Saccharomyces cerevisiae EN DIETAS A BASE DE TRIGO PARA CERDOS

    OpenAIRE

    Ezequiel Reynoso-González; Miguel Cervantes-Ramírez; J. Luis Figueroa-Velasco; Adriana Morales-Trejo; Alfonso Araiza-Piña; Jorge Yáñez-Hernández

    2010-01-01

    La reducción en el contenido de proteína y la inclusión de salvado de trigo en la dieta mejora el perfil de aminoácidos (AA) pero incrementa el contenido de fibra. Al respecto, los cultivos de levadura pueden aumentar la digestión de la fibra. Por tanto, se realizaron tres experimentos para evaluar el efecto de cultivos de levaduras (CL) en dietas con un nivel de proteína cruda estándar (DEPC) o bajo (DBPC), sin o con AA libres, para cerdos (Landrace-Hampshire-Durc) en crecimiento-finalizació...

  12. Implicaciones digestivas y metabólicas del consumo de almidón resistente en el cerdo

    OpenAIRE

    Martínez Puig, Daniel

    2006-01-01

    Descripció del recurs: 4 setembre 2006 Consultable des del TDX La presente tesis doctoral tiene como objetivo general evaluar las principales consecuencias digestivas y metabólicas del consumo de almidón resistente utilizando el cerdo como modelo. Para la consecución de este objetivo se diseñaron tres experimentos. En los 2 primeros se estudiaron los mecanismos de adaptación digestiva y la lipogénesis a medio y largo plazo, y en el tercero el metabolismo postprandial. En todos los exper...

  13. Susceptibilidad antimicrobiana de cepas de Escherichia coli obtenidas de muestras de heces de cerdos destinados a Consumo Humano

    OpenAIRE

    Toledo, Enver; Falcon, Néstor; Flores, Cesar; Rebatta, Mónica; Guevara, José; Ramos, Daphne

    2016-01-01

    Escherichia coli es la especie bacteriana más común de la flora intestinal. La infección de cerdos por parte de cepas de E. coli es un problema frecuente, por lo tanto el conocimiento sobre la susceptibilidad antimicrobiana de estas cepas es importante, debido a que pueden llegar a infectar al ser humano produciendo enfermedades transmitidas por alimentos de difícil tratamiento. El objetivo del presente trabajo fue determinar la sensibilidad antimicrobiana de cepas de E. coli aisladas de cerd...

  14. Phylogeny and ancient DNA of Sus provides insights into neolithic expansion in Island Southeast Asia and Oceania

    Science.gov (United States)

    Larson, Greger; Cucchi, Thomas; Fujita, Masakatsu; Matisoo-Smith, Elizabeth; Robins, Judith; Anderson, Atholl; Rolett, Barry; Spriggs, Matthew; Dolman, Gaynor; Kim, Tae-Hun; Thuy, Nguyen Thi Dieu; Randi, Ettore; Doherty, Moira; Due, Rokus Awe; Bollt, Robert; Djubiantono, Tony; Griffin, Bion; Intoh, Michiko; Keane, Emile; Kirch, Patrick; Li, Kuang-Ti; Morwood, Michael; Pedriña, Lolita M.; Piper, Philip J.; Rabett, Ryan J.; Shooter, Peter; Van den Bergh, Gert; West, Eric; Wickler, Stephen; Yuan, Jing; Cooper, Alan; Dobney, Keith

    2007-01-01

    Human settlement of Oceania marked the culmination of a global colonization process that began when humans first left Africa at least 90,000 years ago. The precise origins and dispersal routes of the Austronesian peoples and the associated Lapita culture remain contentious, and numerous disparate models of dispersal (based primarily on linguistic, genetic, and archeological data) have been proposed. Here, through the use of mtDNA from 781 modern and ancient Sus specimens, we provide evidence for an early human-mediated translocation of the Sulawesi warty pig (Sus celebensis) to Flores and Timor and two later separate human-mediated dispersals of domestic pig (Sus scrofa) through Island Southeast Asia into Oceania. Of the later dispersal routes, one is unequivocally associated with the Neolithic (Lapita) and later Polynesian migrations and links modern and archeological Javan, Sumatran, Wallacean, and Oceanic pigs with mainland Southeast Asian S. scrofa. Archeological and genetic evidence shows these pigs were certainly introduced to islands east of the Wallace Line, including New Guinea, and that so-called “wild” pigs within this region are most likely feral descendants of domestic pigs introduced by early agriculturalists. The other later pig dispersal links mainland East Asian pigs to western Micronesia, Taiwan, and the Philippines. These results provide important data with which to test current models for human dispersal in the region. PMID:17360400

  15. Comportamiento productivo de cerdos en ceba alimentados con follaje fresco de Morus alba como sustituto parcial del concentrado

    Directory of Open Access Journals (Sweden)

    Contino Yuván| Ojeda Félix | Herrera Rafael | Altunaga Nancy | Pérez Guadalupe

    2008-08-01

    Full Text Available El objetivo de esta investigación consistió en evaluar la respuestaproductiva en la etapa de ceba en cerdos mestizos al emplear el follaje de morera fresca (hojas y tallos tiernos como sustituto parcial del concentrado comercial. La investigación se efectuó en el Módulo de Investigación-Producción Porcina de la EEPF ”Indio Hatuey” Se evaluaron las ganancias de peso vivo de cerdos en crecimiento desde los 30 kg hasta los 90 kg. El pesaje se realizó cada 14 días, con la determinación del peso vivo promedio, la ganancia media diaria, y la conversión alimentaría. Para el procesamiento se empleó el paquete estadístico SPSS versión 10.1. Se obtuvo un incremento de las ganancias medias diarias con el aumento del peso vivo y una mejor eficiencia en la dieta total por la utilización de lamorera. Los resultados demuestran la factibilidad económica de estaalternativa alimentaria

  16. PREVALENCIA SEROLÓGICA DEL SÍNDROME REPRODUCTIVO Y RESPIRATORIO PORCINO (PRRS EN CERDOS DE EXPLOTACIONES EXTENSIVAS DE COLOMBIA

    Directory of Open Access Journals (Sweden)

    Rincón M. A.

    2006-11-01

    Full Text Available El presente trabajo tuvo como objetivo actualizar en el país la situación de la infección por el virus del Síndrome reproductivo y respiratorio porcino (PRRS. Los departamentos incluidos en el estudio fueron aquellos donde se manejan producciones extensivas o de traspatio. Las muestras fueron tomadas de forma aleatoria en los mataderos de cada departamento, analizándose un total de 1.658 sueros, los cuales fueron clasiicados, hasta donde fue posible, de acuerdo a las categorías productivas (cerdos de descarte y cerdos de ceba. Las muestras obtenidas se analizaron a través de una prueba de ELISA, usando el kit comercial HerdChek PRRS 2XR (Laboratorios IDEXX. Al inal se obtuvo un total de 71 sueros reactores, lo que se traduce en una prevalencia del 4,3 ± 1,0% (p≤0,05. Los departamentos con mayor prevalencia fueron Norte de Santander y Arauca, mientras que los departamentos de La Guajira, Magdalena y Sucre mantuvieron su condición de no reactividad serológica en estos sistemas de producción.

  17. PREVALENCIA SEROLÓGICA DEL SÍNDROME REPRODUCTIVO Y RESPIRATORIO PORCINO (PRRS EN CERDOS DE EXPLOTACIONES EXTENSIVAS DE COLOMBIA

    Directory of Open Access Journals (Sweden)

    MC Cruz

    2006-01-01

    Full Text Available El presente trabajo tuvo como objetivo actualizar en el país la situación de la infección por el virus del Síndrome reproductivo y respiratorio porcino (PRRS. Los departamentos incluidos en el estudio fueron aquellos donde se manejan producciones extensivas o de traspatio. Las muestras fueron tomadas de forma aleatoria en los mataderos de cada departamento, analizándose un total de 1.658 sueros, los cuales fueron clasiicados, hasta donde fue posible, de acuerdo a las categorías productivas (cerdos de descarte y cerdos de ceba. Las muestras obtenidas se analizaron a través de una prueba de ELISA, usando el kit comercial HerdChek PRRS 2XR (Laboratorios IDEXX. Al inal se obtuvo un total de 71 sueros reactores, lo que se traduce en una prevalencia del 4,3 ± 1,0% (p≤0,05. Los departamentos con mayor prevalencia fueron Norte de Santander y Arauca, mientras que los departamentos de La Guajira, Magdalena y Sucre mantuvieron su condición de no reactividad serológica en estos sistemas de producción.

  18. Domestic pigs' (Sus scrofa domestica) use of direct and indirect visual and auditory cues in an object choice task.

    Science.gov (United States)

    Nawroth, Christian; von Borell, Eberhard

    2015-05-01

    Recently, foraging strategies have been linked to the ability to use indirect visual information. More selective feeders should express a higher aversion against losses compared to non-selective feeders and should therefore be more prone to avoid empty food locations. To extend these findings, in this study, we present a series of studies investigating the use of direct and indirect visual and auditory information by an omnivorous but selective feeder-the domestic pig. Subjects had to choose between two buckets, with only one containing a reward. Before making a choice, the subjects in Experiment 1 (N = 8) received full information regarding both the baited and non-baited location, either in a visual or auditory domain. In this experiment, the subjects were able to use visual but not auditory cues to infer the location of the reward spontaneously. Additionally, four individuals learned to use auditory cues after a period of training. In Experiment 2 (N = 8), the pigs were given different amounts of visual information about the content of the buckets-lifting either both of the buckets (full information), the baited bucket (direct information), the empty bucket (indirect information) or no bucket at all (no information). The subjects as a group were able to use direct and indirect visual cues. However, over the course of the experiment, the performance dropped to chance level when indirect information was provided. A final experiment (N = 3) provided preliminary results for pigs' use of indirect auditory information to infer the location of a reward. We conclude that pigs at a very young age are able to make decisions based on indirect information in the visual domain, whereas their performance in the use of indirect auditory information warrants further investigation.

  19. Molecular characterization and seroprevalence of Echinococcus granulosus in wild boars (Sus scrofa) in south-western Iran.

    Science.gov (United States)

    Sarkari, Bahador; Mansouri, Majid; Khabisi, Samaneh Abdolahi; Mowlavi, Gholamreza

    2015-01-01

    This study presents the first molecular and serological evaluation of Echinococcus granulosus infections in wild boars in Iran. Twenty five wild boars were collected in south-western Iran, during authorized hunting program, from March to October 2013, necropsied and examined for E. granulosus infection. Furthermore, seroprevalence of cystic echinococcosis in hunted boars was evaluated by an ELISA system. A fertile hydatid cyst due to E. granulosus was detected in the lung of one of the animals. Genotype analysis of the isolate was determined by analyzing a mitochondrial gene, cytochrome C oxidase subunit 1 (co1). DNA was extracted from the cyst sample and polymerase chain reaction amplification and DNA sequencing of the specific region of the co1 gene was performed. Molecular evaluation confirmed the presence of a sheep strain, the G1 genotype, in the wild boar in south-western Iran. This is the first report of the presence of G1 genotype of E. granulosus in wild boar in Iran. Serological evaluation of hydatid cyst by antigen-B ELISA revealed E. granulosus antibodies in 5 (20%) of 25 wild boars. A statistically significant difference was observed between the prevalence of E. granulosus antibodies and gender while the difference between the seroprevalence of E. granulosus and age was insignificant. Findings of this study might have important implications for the prevention and control of cystic echinococcosis.

  20. The characteristics of the porcine (Sus scrofa) liver miRNAome with the use of next generation sequencing.

    Science.gov (United States)

    Pawlina, Klaudia; Gurgul, Artur; Oczkowicz, Maria; Bugno-Poniewierska, Monika

    2015-05-01

    MicroRNAs (miRNAs) are a class of small, noncoding RNAs, which play a vital role in the regulation of gene expression by binding to the 3' untranslated region (3'UTR) of a target mRNA. Despite a significant improvement in the identification of miRNAs in a variety of species, the coverage of the porcine miRNAome is still scarce. To identify porcine miRNAs potentially regulating processes taking place in the liver, we applied next generation sequencing. As a result, we detected 206 distinct miRNAs, of which 68 represented potential novel miRNAs. Among these new miRNAs, there were miRNAs deriving from the opposite arm of a hairpin precursor of already known miRNAs. Moreover, we observed 3' and 5' length and sequence variants, probably constituting so called isomiRs, as well as differentially mapped precursor loci, alternative precursor sequences and clustering of miRNA encoding genes. On the basis of expression levels, reflected by the number of sequence reads, we identified the most abundant miRNAs followed by gene target prediction and pathway analysis. The enriched pathways were connected with cellular and metabolic processes, growth factors as well as enzymatic activity. The obtained results are the first ones to concern the porcine liver miRNAome. Consequently, they will increase the number of known porcine miRNAs and facilitate further research on gene regulation mechanisms as well as biological processes associated with the liver functioning in pigs.

  1. The effect of selected environmental Fusarium mycotoxins on the ovaries in the female wild boar (Sus scrofa).

    Science.gov (United States)

    Zielonka, Ł; Gajęcka, M; Żmudzki, J; Gajęcki, M

    2015-01-01

    The contamination of agricultural crops with Fusarium mycotoxins poses one of the greatest problems in food production. Wild boars live in specific habitats and are physiologically sensitive to Fusarium mycotoxins, therefore, they are an interesting model for studies investigating the effects of the discussed toxin, in particular under low-dose exposure. The objective of this study was to determine potential effects of Fusarium mycotoxins ingested with naturally contaminated food on reproductive function based on the proliferation and apoptotic indices of ovarian follicles in female wild boars. The experiment was conducted on 40 wild boars inhabiting north-eastern Poland. The effect of seasonal variations in the quantity and quality of ingested food on the concentrations of Fusarium mycotoxins and their metabolites in the blood of wild boars was analyzed. The observed differences in toxin levels were accompanied by changes in proliferation and apoptotic indices. Proliferation processes were most intense in autumn-winter and were least advanced in winter-spring. The intensity of apoptotic processes was inversely correlated with proliferation.

  2. An Effective and Reproducible Model of Ventricular Fibrillation in Crossbred Yorkshire Swine (Sus scrofa) for Use in Physiologic Research.

    Science.gov (United States)

    Burgert, James M; Johnson, Arthur D; Garcia-Blanco, Jose C; Craig, W John; O'Sullivan, Joseph C

    2015-10-01

    Transcutaneous electrical induction (TCEI) has been used to induce ventricular fibrillation (VF) in laboratory swine for physiologic and resuscitation research. Many studies do not describe the method of TCEI in detail, thus making replication by future investigators difficult. Here we describe a detailed method of electrically inducing VF that was used successfully in a prospective, experimental resuscitation study. Specifically, an electrical current was passed through the heart to induce VF in crossbred Yorkshire swine (n = 30); the current was generated by using two 22-gauge spinal needles, with one placed above and one below the heart, and three 9V batteries connected in series. VF developed in 28 of the 30 pigs (93%) within 10 s of beginning the procedure. In the remaining 2 swine, VF was induced successfully after medial redirection of the superior parasternal needle. The TCEI method is simple, reproducible, and cost-effective. TCEI may be especially valuable to researchers with limited access to funding, sophisticated equipment, or colleagues experienced in interventional cardiology techniques. The TCEI method might be most appropriate for pharmacologic studies requiring VF, VF resulting from the R-on-T phenomenon (as in prolonged QT syndrome), and VF arising from other ectopic or reentrant causes. However, the TCEI method does not accurately model the most common cause of VF, acute coronary occlusive disease. Researchers must consider the limitations of TCEI that may affect internal and external validity of collected data, when designing experiments using this model of VF.

  3. [Genetic components and the uncertainty of the phenotypic realization of the mass of newborns in domestic pigs Sus scrofa L].

    Science.gov (United States)

    Nikitin, S V; Kniazev, S P; Ermolaev, V I

    2014-01-01

    In this article, we discuss the features of the genetic determination of a continuous quantitative trait, the mass of newborn offspring in populations of the domestic pig. We defined several components that determine the phenotypic trait, such as the maternal effect, complete dominance, interaction of the parental alleles in the genotype of the offspring, and the uncertainty of phenotypic realization of genotype. We found that a phenotypic trait of high genetic determinacy can also have a maximum range in phenotypic realization, in which case each genotype encountered in the population can realize within the entire range of possible phenotypes.

  4. Echinococcus granulosus genotypes circulating in alpacas (Lama pacos and pigs (Sus scrofa from an endemic region in Peru

    Directory of Open Access Journals (Sweden)

    Elizabeth Sánchez

    2012-03-01

    Full Text Available The identification of the genotypes of Echinococcus granulosus present in livestock and wild animals within regions endemic for cystic echinococcosis (CE is epidemiologically important. Individual strains display different biological characteristics that contribute to outbreaks of CE and that must be taken into account in the design of intervention programs. In this study, samples of hydatid cysts due to E. granulosus were collected from alpacas (4 in Puno and pigs (8 in Ayacucho in Peru, an endemic region for CE. Polymerase chain reaction amplification and DNA sequencing of specific regions of the mitochondrial cytochrome C oxidase subunit 1 and NADH dehydrogenase subunit 1 genes confirmed the presence of a strain common to sheep, the G1 genotype, in alpacas. Two different strains of E. granulosus were identified in pigs: the G1 and the G7 genotypes. This is the first report of the G1 genotype of E. granulosus in alpacas in endemic regions of CE in Peru.

  5. The first pathogenic Yersinia enterocolitica bioserotype 4/O:3 strain isolated from a hunted wild boar (Sus scrofa) in Poland.

    Science.gov (United States)

    Bancerz-Kisiel, A; Platt-Samoraj, A; Szczerba-Turek, A; Syczyło, K; Szweda, W

    2015-10-01

    The objective of this study was to identify the bioserotypes and virulence markers of Yersinia enterocolitica strains isolated from wild boars in Poland. Bacteriological examination of 302 rectal swabs from 151 wild boars resulted in the isolation of 40 Y. enterocolitica strains. The majority of the examined strains (n = 30), belonged to bioserotype 1A/NI. The presence of individual Y. enterocolitica strains belonging to bioserotypes 1B/NI (3), 1A/O:8 (2), 1A/O:27 (2), 2/NI (1), 2/O:9 (1) and 4/O:3 (1) was also demonstrated. Amplicons corresponding to ail and ystA genes were observed only in one Y. enterocolitica strain--bioserotype 4/O:3. The ail and ystB gene amplicons were noted in 11 Y. enterocolitica biotype 1A strains, although single amplicons of ystB gene were found in 28 of the tested samples. In four out of eight cases when two Y. enterocolitica strains were isolated from the same animal, the strains differed in biotype, serotype or virulence markers. The European population of wild boars continues to grow and spread to new areas, therefore, wild boars harbouring potentially pathogenic Y. enterocolitica 4/O:3 strains pose a challenge to public health.

  6. An in vivo three-dimensional magnetic resonance imaging-based averaged brain collection of the neonatal piglet (Sus scrofa).

    Science.gov (United States)

    Conrad, Matthew S; Sutton, Bradley P; Dilger, Ryan N; Johnson, Rodney W

    2014-01-01

    Due to the fact that morphology and perinatal growth of the piglet brain is similar to humans, use of the piglet as a translational animal model for neurodevelopmental studies is increasing. Magnetic resonance imaging (MRI) can be a powerful tool to study neurodevelopment in piglets, but many of the MRI resources have been produced for adult humans. Here, we present an average in vivo MRI-based atlas specific for the 4-week-old piglet. In addition, we have developed probabilistic tissue classification maps. These tools can be used with brain mapping software packages (e.g. SPM and FSL) to aid in voxel-based morphometry and image analysis techniques. The atlas enables efficient study of neurodevelopment in a highly tractable translational animal with brain growth and development similar to humans.

  7. Novel Techniques for Retroperitoneal Implantation of Telemetry Transmitters for Physiologic Monitoring in Gottingen Minipigs (Sus scrofa domesticus)

    Science.gov (United States)

    2014-12-01

    interference from the impedance leads. The subcutaneous layer, tacked down to the external sheath of the linea alba to reduce dead space , was closed...2014 468 to paramedian implantation between the external and internal abdominal musculature, as typically is performed in our African green monkeys ...McMonagle JD, McGinley MJ, Evans J. 2007. The toxicity of soman in the African green monkey (Chlorocebus aethiops). Toxicol Mech Methods 17:255–264

  8. Imprinting Analysis of RTL1 and DI03 Genes and Their Association with Carcass Traits in Pigs (Sus scrofa)

    Institute of Scientific and Technical Information of China (English)

    YANG Zong-lin; CHENG Huan-chen; XIA Qing-you; JIANG Cao-de; DENG Chang-yan; LI Yue-min

    2009-01-01

    Imprinted genes play significant roles in the regulation of fetal growth,development,function of the placenta and postnatal behavior in mammals,but little is known in pigs.In order to investigate the imprinting status of porcine retro-transposon like 1 (RTL1) and type 3 iodothyronine deiodinase (D103) genes,DNA samples of the parents and F1 animals,generated with reciprocal crosses between Large White and Meishan breeds,were isolated,and analyzed by reverse transcription polymerase chain reaction restriction fragment length polymorphism (RT-PCR-RFLP).The results demonstrated that the RTL1 gene was paternally expressed in 10 tissues,such as the skeletal muscle,heart,spleen,liver,kidney,lung,stomach,fat,small intestine and brain,and DI03 gene exhibited paternal expression in the skeletal muscle,heart,spleen,lung,stomach,and brain,in 2-month-old pigs.The association of RTL1 and DIO3 with carcass traits was further analyzed in the F2 population of Large White × Meishan pigs.The statistical results showed that the RTL1 A1101G polymorphism (EU781029) was significantly associated with lean meat percentage (LMP) and fat meat percentage (FMP) (P<0.05),while the DIO3 A744C polymorphism (AY533208) was not significantly associated with any carcass traits.These results indicate that the imprinting status of RTL1 and DIO3 is well kept across the mammalian species,and porcine RTL1 may have important roles in muscle growth and fat deposition.

  9. Untangling the hybrid nature of modern pig genomes: a mosaic derived from biogeographically distinct and highly divergent Sus scrofa populations.

    Science.gov (United States)

    Bosse, Mirte; Megens, Hendrik-Jan; Madsen, Ole; Frantz, Laurent A F; Paudel, Yogesh; Crooijmans, Richard P M A; Groenen, Martien A M

    2014-08-01

    The merging of populations after an extended period of isolation and divergence is a common phenomenon, in natural settings as well as due to human interference. Individuals with such hybrid origins contain genomes that essentially form a mosaic of different histories and demographies. Pigs are an excellent model species to study hybridization because European and Asian wild boars diverged ~1.2 Mya, and pigs were domesticated independently in Europe and Asia. During the Industrial Revolution in England, pigs were imported from China to improve the local pigs. This study utilizes the latest genomics tools to identify the origin of haplotypes in European domesticated pigs that are descendant from Asian and European populations. Our results reveal fine-scale haplotype structure representing different ancient demographic events, as well as a mosaic composition of those distinct histories due to recently introgressed haplotypes in the pig genome. As a consequence, nucleotide diversity in the genome of European domesticated pigs is higher when at least one haplotype of Asian origin is present, and haplotype length correlates negatively with recombination frequency and nucleotide diversity. Another consequence is that the inference of past effective population size is influenced by the background of the haplotypes in an individual, but we demonstrate that by careful sorting based on the origin of haplotypes, both distinct demographic histories can be reconstructed. Future detailed mapping of the genomic distribution of variation will enable a targeted approach to increase genetic diversity of captive and wild populations, thus facilitating conservation efforts in the near future.

  10. Proteomic analysis of pig (Sus scrofa olfactory soluble proteome reveals O-GlcNAcylation of secreted odorant-binding proteins

    Directory of Open Access Journals (Sweden)

    Patricia eNAGNAN-LE MEILLOUR

    2014-12-01

    Full Text Available The diversity of olfactory binding proteins (OBPs is a key point to understand their role in molecular olfaction. Since only few different sequences were characterized in each mammalian species, they have been considered as passive carriers of odors and pheromones. We have explored the soluble proteome of pig nasal mucus, taking benefit of the powerful tools of proteomics. Combining two-dimensional electrophoresis, mass spectrometry and western-blot with specific antibodies, our analyses revealed for the first time that the pig nasal mucus is mainly composed of secreted OBP isoforms, some of them being potentially modified by O-GlcNAcylation. An ortholog gene of the glycosyltransferase responsible of the O-GlcNAc linking on extracellular proteins in Drosophila and Mouse (EOGT was amplified from tissues of pigs of different ages and sex. The sequence was used in a phylogenetic analysis, which evidenced conservation of EOGT in insect and mammalian models studied in molecular olfaction. Extracellular O-GlcNAcylation of secreted OBPs could finely modulate their binding specificities to odors and pheromones. This constitutes a new mechanism for extracellular signaling by OBPs, suggesting that they act as the first step of odor discrimination.

  11. An in vivo three-dimensional magnetic resonance imaging-based averaged brain collection of the neonatal piglet (Sus scrofa.

    Directory of Open Access Journals (Sweden)

    Matthew S Conrad

    Full Text Available Due to the fact that morphology and perinatal growth of the piglet brain is similar to humans, use of the piglet as a translational animal model for neurodevelopmental studies is increasing. Magnetic resonance imaging (MRI can be a powerful tool to study neurodevelopment in piglets, but many of the MRI resources have been produced for adult humans. Here, we present an average in vivo MRI-based atlas specific for the 4-week-old piglet. In addition, we have developed probabilistic tissue classification maps. These tools can be used with brain mapping software packages (e.g. SPM and FSL to aid in voxel-based morphometry and image analysis techniques. The atlas enables efficient study of neurodevelopment in a highly tractable translational animal with brain growth and development similar to humans.

  12. EPINEPHRINE CONCENTRATION IN WILD BOAR (SUS SCROFA L. SERUM AFTER REPEATED ELISA TESTED FREEZE-THAWING CYCLES

    Directory of Open Access Journals (Sweden)

    Neška Vukšić

    2013-06-01

    Full Text Available Blood samples for determining of epinephrine concentration and biochemical parameters in the blood serum of wild boars were taken from 42 healthy wild boars, both sexes, during the hunting season. All animals in good condition, body weight 20 to 95 kg, were divided into two groups up to 50 kg (group A and up to 95 kg (Group B. Epinephrine concentration was determined by ELISA twice: one week after taking samples and a month after repeated freezing at -80°C. It was higher in relation to the reference value of domestic pigs and human (109.45 pg/ml in A and 119.54 pg/ml in B group. Repeated freezing and re-analysis after a month were resulted in lower concentrations of epinephrine (12% in young and 11.17% in adult animal, but without statistical significance (P>0.05. Biochemical analysis results’ show increased glucose and triglycerides concentrations compared to the reference values, while other indicators were observed within or slightly increased referring to the normal range. The correlation between glucose and epinephrine was not determined.

  13. A statistical GIS-based analysis of Wild boar (Sus scrofa traffic collisions in a Mediterranean area

    Directory of Open Access Journals (Sweden)

    Andrea Amici

    2010-01-01

    Full Text Available vThis study was aimed at analysing the most relevant environmental variables involved in vehicle–wild boar road collisions in a Mediterranean area, starting from a territorial information system implemented in a GIS environment. Landscape structure indices and local qualitative and quantitative variables were correlated to identify the most frequent predisposing factors of collisions. Statistical tests of the considered parameters indicated a higher frequency of collisions in the evening hours of late summer and autumn (P<0.05 compared with daytime and night-time, localized nearness to attraction points (feeding or resting sites; P<0.001 and closeness to no-hunting areas (P<0.001. In addition, local road parameters (shape of road, visibility at road sides, etc. considerably increased the risk of collisions.

  14. Damage caused by red deer (Cervus elaphus & wild boar (Sus scrofa in forest hunting grounds in Serbia

    Directory of Open Access Journals (Sweden)

    Gačić Dragan P.

    2009-01-01

    Full Text Available The systematic study and assessment of the damage by big game in forest hunting grounds in Serbia was infrequent, although the damage was evident. The objective of this paper is to identify the rates and types of damage by red deer and wild boar at three localities: (1 fenced part of the hunting ground 'Crni Lug' (Srem, (2 fenced part of the hunting ground 'Podunavsko Lovište Plavna' (Southwestern Bačka, and (3 fenced rearing centre 'Lomnička Reka' (Mt. Veliki Jastrebac. The damage was not recorded on locality (1. The damage on locality (2 (new polar plantations and locality (3 (beech forests was caused by red deer. The main causes of the damage were excessive density and disturbed population structure (sex and age, nonharmonised forest and hunting management, shortage of natural food, especially of pasture areas.

  15. Seroprevalence and parasite load of Toxoplasma gondii in Mexican hairless pig (Sus scrofa) tissues from the Southeast of Mexico.

    Science.gov (United States)

    Dzib-Paredes, G F; Rosado-Aguilar, J A; Acosta-Viana, K Y; Ortega-Pacheco, A; Hernández-Cortázar, I B; Guzman-Marín, E; Jiménez-Coello, M

    2016-10-15

    This study aimed to estimate the seroprevalence and determine the parasite load of Toxoplasma gondii (T. gondii) in the heart and tongue tissues and the semimembranosus/gracilis muscles in a population of Mexican hairless pig (MHP). A cross-sectional study was conducted in 81 MHP, 9-12 weeks of age, from 10 municipalities in Yucatan, Mexico. The prevalence was estimated by the detection of T. gondii IgG antibodies via an indirect ELISA assay. The parasite burden was also determined by testing genomic DNA from sampled tissues using quantitative real-time PCR (qPCR), which amplified a 62bp product from the B1 gene of T. gondii. The seroprevalence was 53% (43/81), ranging from 0 to 100% seropositivity rate depending on the municipalitie of origin. The qPCR allowed detection of T. gondii in 5.3% (9/168) of the tissue samples, with an average of 2.5±2.71 parasites per gram (0.17±0.18 parasite equivalent) for the leg muscle tissue, 0.26±0.39 (0.01±0.02 parasite equivalent) for the heart tissue and 0.31±0.37 (0.021±0.025 parasite equivalent) for the tongue tissue. No significant difference (p>0.05) was observed in the proportion and parasite burden among the different types of tissues evaluated. As shown by the ELISA, a high seroprevalence of T. gondii exists in MHP from the Yucatán Peninsula. The parasite burden found in the tissues showed similar tropism; therefore, the consumption of these tissues involves the same risk of acquiring infection if not properly cooked.

  16. Hydroxocobalamin Versus Sodium Thiosulfate for the Treatment of Acute Cyanide Toxicity in a Swine (Sus scrofa) Model

    Science.gov (United States)

    2012-06-01

    treatment of acute cyanide poisoning in adult beagle dogs . Clin Toxicol (Phila). 2006;44(suppl 1):5-15. 15. Posner MA, Tobey RE, McElroy H...cobalamine and acute cyanide poisoning in dogs . Life Sci. 1965;4:1785-1789. 18. Borron SW, Baud FJ, Barriot P, et al. Prospective study of hydroxocobalamin... Cardiology , the European Resuscitation Council, the Heart and Stroke Foundation of Canada, the Institute of Critical Care Medicine, the Safar Center for

  17. Comparison of Microchip Transponder and Noncontact Infrared Thermometry with Rectal Thermometry in Domestic Swine (Sus scrofa domestica)

    Science.gov (United States)

    Jara, Amanda L; Hanson, Jarod M; Gabbard, Jon D; Johnson, Scott K; Register, Emery T; He, Biao

    2016-01-01

    During disease outbreaks, core temperature is a useful health metric in swine, due to the presence of pyrexia especially during the acute phase of infection. Despite technologic advances in other facets of swine production and health management, rectal thermometry continues to be the ‘gold standard’ for measuring core body temperature. However, for various reasons, collecting rectal temperatures can be difficult and unsafe depending on the housing modality. In addition, the delay between insertion of the rectal thermometer and obtaining a reading can affect measurement accuracy, especially when the pig requires physical restraint. Clearly safer, faster, and more accurate and precise temperature acquisition methods that necessitate minimal or no handling of swine are needed. We therefore compared rectal thermometers, subcutaneous microchips, and an inexpensive handheld infrared thermometer by measuring the core body temperature of 24 male castrated piglets at random intervals over a 5-wk period. The core body temperature (mean ± 1 SD) was 39.3 ± 0.5 °C by rectal thermometry, 39.0 ± 0.7 °C by microchip transponder, and 34.3 ± 1.0 °C by infrared thermometry; these 3 values differed significantly. Although the readings obtain by using infrared thermometry were numerically lower than those from the other methods, it is arguably the safest method for assessing the core temperature of swine and showed strong relative correlation with rectal temperature. PMID:27657715

  18. Wildlife genetics and disease: allozyme evolution in the wild boar (Sus scrofa caused by a swine fever epidemy

    Directory of Open Access Journals (Sweden)

    Schreiber Arnd

    2000-05-01

    Full Text Available Abstract Enzyme polymorphism at 42 loci was compared before and after a major epidemy of swine fever in wild boars from northern Vosges (France. No change was observed in the 38 monomorphic loci, but allele frequencies at the phosphoglucomutase locus PGM-2* changed significantly. Possible causes for this observation are discussed, and it appears that PGM-2 locus could be a genetic marker of resistance to this viral disease.

  19. Comparison of Microchip Transponder and Noncontact Infrared Thermometry with Rectal Thermometry in Domestic Swine (Sus scrofa domestica).

    Science.gov (United States)

    Jara, Amanda L; Hanson, Jarod M; Gabbard, Jon D; Johnson, Scott K; Register, Emery T; He, Biao; Tompkins, S Mark

    2016-01-01

    During disease outbreaks, core temperature is a useful health metric in swine, due to the presence of pyrexia especially during the acute phase of infection. Despite technologic advances in other facets of swine production and health management, rectal thermometry continues to be the 'gold standard' for measuring core body temperature. However, for various reasons, collecting rectal temperatures can be difficult and unsafe depending on the housing modality. In addition, the delay between insertion of the rectal thermometer and obtaining a reading can affect measurement accuracy, especially when the pig requires physical restraint. Clearly safer, faster, and more accurate and precise temperature acquisition methods that necessitate minimal or no handling of swine are needed. We therefore compared rectal thermometers, subcutaneous microchips, and an inexpensive handheld infrared thermometer by measuring the core body temperature of 24 male castrated piglets at random intervals over a 5-wk period. The core body temperature (mean ± 1 SD) was 39.3±0.5 °C by rectal thermometry, 39.0±0.7 °C by microchip transponder, and 34.3±1.0 °C by infrared thermometry; these 3 values differed significantly. Although the readings obtain by using infrared thermometry were numerically lower than those from the other methods, it is arguably the safest method for assessing the core temperature of swine and showed strong relative correlation with rectal temperature.

  20. A Comparative Study of Melanin Content and Skin Morphology for Three Commonly Used Laboratory Swine (Sus scrofa domestica)

    Science.gov (United States)

    2012-09-01

    that immune cells (i.e., phagocytes) can move into this layer to respond to bacteria and/or other foreign bodies, which could penetrate the skin. The...mirror images) from the bisected skin biopsy were usually available on each slide. A coin was tossed to determine which section would be imaged. If...was aligned so that the epidermal surface of the skin was approximately perpendicular to the long axis of the image. A second coin was tossed to

  1. Evaluation of ELISA coupled with Western blot as a surveillance tool for Trichinella infection in wild boar (Sus scrofa).

    Science.gov (United States)

    Cuttell, Leigh; Gómez-Morales, Maria Angeles; Cookson, Beth; Adams, Peter J; Reid, Simon A; Vanderlinde, Paul B; Jackson, Louise A; Gray, C; Traub, Rebecca J

    2014-01-31

    Trichinella surveillance in wildlife relies on muscle digestion of large samples which are logistically difficult to store and transport in remote and tropical regions as well as labour-intensive to process. Serological methods such as enzyme-linked immunosorbent assays (ELISAs) offer rapid, cost-effective alternatives for surveillance but should be paired with additional tests because of the high false-positive rates encountered in wildlife. We investigated the utility of ELISAs coupled with Western blot (WB) in providing evidence of Trichinella exposure or infection in wild boar. Serum samples were collected from 673 wild boar from a high- and low-risk region for Trichinella introduction within mainland Australia, which is considered Trichinella-free. Sera were examined using both an 'in-house' and a commercially available indirect-ELISA that used excretory-secretory (E/S) antigens. Cut-off values for positive results were determined using sera from the low-risk population. All wild boar from the high-risk region (352) and 139/321 (43.3%) of the wild boar from the low-risk region were tested by artificial digestion. Testing by Western blot using E/S antigens, and a Trichinella-specific real-time PCR was also carried out on all ELISA-positive samples. The two ELISAs correctly classified all positive controls as well as one naturally infected wild boar from Gabba Island in the Torres Strait. In both the high- and low-risk populations, the ELISA results showed substantial agreement (k-value=0.66) that increased to very good (k-value=0.82) when WB-positive only samples were compared. The results of testing sera collected from the Australian mainland showed the Trichinella seroprevalence was 3.5% (95% C.I. 0.0-8.0) and 2.3% (95% C.I. 0.0-5.6) using the in-house and commercial ELISA coupled with WB respectively. These estimates were significantly higher (PTrichinella DNA in any mainland animals, but did reveal the presence of a second larvae-positive wild boar on Gabba Island, supporting its utility as an alternative, highly sensitive method in muscle examination. The serology results suggest Australian wildlife may have been exposed to Trichinella parasites. However, because of the possibility of non-specific reactions with other parasitic infections, more work using well-defined cohorts of positive and negative samples is required. Even if the specificity of the ELISAs is proven to be low, their ability to correctly classify the small number of true positive sera in this study indicates utility in screening wild boar populations for reactive sera which can be followed up with additional testing.

  2. Detection of Rickettsia tamurae DNA in ticks and wild boar (Sus scrofa leucomystax) skins in Shimane Prefecture, Japan.

    Science.gov (United States)

    Motoi, Yuta; Asano, Makoto; Inokuma, Hisashi; Ando, Shuji; Kawabata, Hiroki; Takano, Ai; Suzuki, Masatsugu

    2013-01-01

    We used 24 wild boars trapped from December 2009 to January 2010 and a further 65 from July 2010 to August 2010 in Misato Town, Shimane Prefecture, Japan. We collected blood, spleens, skins and ticks from the wild boars, which were examined for rickettsial infections using polymerase chain reaction (PCR) primers for the genes rickettsial 17-kDa antigen and citrate synthase (gltA). We amplified Rickettsia tamurae AT-1 DNA from the tick Amblyomma testudinarium and from wild boar skins where ticks attached. Antibodies against spotted fever group Rickettsia were detected in wild boar sera using immunofluorescence, whereas blood and spleen samples contained no rickettsial DNA. This study suggests that wild boars have a role as an amplifier and a transporter of A. testudinarium, which harbor R. tamurae. One case of R. tamurae infection in humans was reported in Shimane Prefecture. Therefore, R. tamurae infections in humans might increase, if wild boar populations and their habitats expand.

  3. Association of Oxelytrum cayennense (Silphidae, Coleoptera with Pig Carcasses (Sus scrofa, Suidae in Terra Firme Areas in Manaus, Amazonas, Brazil

    Directory of Open Access Journals (Sweden)

    Alexandre Ururahy-Rodrigues

    2010-07-01

    Resumo. Aspectos da colonização do silfídeo Oxelytrum cayennense (Sturm em carcaças de porcos do tamanho de um homem adulto na Reserva Florestal Adolpho Ducke são apresentados. Esta espécie colonizou as carcaças de porcos do estágio enfisematoso à esqueletização. Adultos foram observados eventualmente predando larvas de moscas a partir do segundo dia de intervalo pós-morte e suas larvas foram observadas a partir do quinto dia se alimentando em áreas esqueletizadas, provavelmente limpando os ossos dos tecidos moles remanescentes. O comportamento predatório dos adultos nas larvas de dípteros mais velhas podem impactar suas populações e complicar estimativas de intervalo pós-morte, uma vez que estas são freqüentemente usadas para esta estimativa. Além disso, adultos e larvas têm potencial uso como indicadores de intervalo pós-morte (IPM porque o adulto pode colonizar carcaças do segundo dia em diante e as larvas do quinto dia em diante.

  4. Untangling the hybrid nature of modern pig genomes: a mosaic derived from biogeographically distinct and highly divergent Sus scrofa populations

    NARCIS (Netherlands)

    Bosse, M.; Megens, H.J.W.C.; Madsen, O.; Frantz, L.A.F.; Paudel, Y.; Crooijmans, R.P.M.A.; Groenen, M.

    2014-01-01

    The merging of populations after an extended period of isolation and divergence is a common phenomenon, in natural settings as well as due to human interference. Individuals with such hybrid origins contain genomes that essentially form a mosaic of different histories and demographies. Pigs are an e

  5. The challenge of detecting classical swine fever virus circulation in wild boar (Sus scrofa): Simulation of sampling options

    DEFF Research Database (Denmark)

    Schulz, Jana; Schulz, Katja; Blome, Sandra;

    2016-01-01

    populations can be a major cause of primary outbreaks in domestic pigs, strict control measures for both species were implemented. To guarantee early detection and to demonstrate freedom from disease, intensive surveillance is carried out based on a hunting bag sample. In this context, virologic...... investigations play a major role in the early detection of new introductions and in regions immunized with a conventional vaccine. The required financial resources and personnel for reliable testing are often large, and sufficient sample sizes to detect low virus prevalences are difficult to obtain. We conducted...... a simulation to model the possible impact of changes in sample size and sampling intervals on the probability of CSF virus detection based on a study area of 65 German hunting grounds. A 5-yr period with 4,652 virologic investigations was considered. Results suggest that low prevalences could not be detected...

  6. Social Complexification and Pig (Sus scrofa) Husbandry in Ancient China: A Combined Geometric Morphometric and Isotopic Approach.

    Science.gov (United States)

    Cucchi, Thomas; Dai, Lingling; Balasse, Marie; Zhao, Chunqing; Gao, Jiangtao; Hu, Yaowu; Yuan, Jing; Vigne, Jean-Denis

    2016-01-01

    Pigs have played a major role in the economic, social and symbolic systems of China since the Early Neolithic more than 8,000 years ago. However, the interaction between the history of pig domestication and transformations in Chinese society since then, have not been fully explored. In this paper, we investigated the co-evolution from the earliest farming communities through to the new political and economic models of state-like societies, up to the Chinese Empire, using 5,000 years of archaeological records from the Xiawanggang (XWG) and Xinzhai (XZ) sites (Henan Province). To trace the changes of pig populations against husbandry practices, we combined the geometric morphometric analysis of dental traits with a study of the stable carbon and nitrogen isotope ratios from bone collagen. The domestication process intensified during the Neolithic Yangshao, prompted by greater selective pressure and/or better herd control against wild introgression. After that, pig farming, in XWG, relied on local livestock and a gradual change of husbandry practices overtime. This was characterized by a gentle increase in millet foddering and animal protein intake, until a complete change over to household management during the Han dynasty. The only rupture in this steady trend of husbandry occurred during the Longshan period, with the appearance of small sized and idiosyncratic pigs with specific feeding practices (relying on millet and household scraps). From three exploratory hypothesis, we explored the possibility of anti-elite pig production in XWG during the Longshan period, as a means to resist incorporation into a new economic model promoting intensified domestic production. This exploratory hypothesis is the most suitable to our dataset; however, numerous areas need to be explored further in order to adequately document the role of pigs in the rise of China's complex societies.

  7. SUS – 20 anos

    Directory of Open Access Journals (Sweden)

    Carlos Eduardo Aguilera Campos

    2010-11-01

    Full Text Available A RBMFC não poderia deixar de fazer alusão ao aniversário de 20 anos da promulgação do Sistema Único de Saúde (SUS. Este momento tem sido motivo de reflexão por todos aqueles que acompanham e militam, como nós, na sua construção. Em primeiro lugar cremos ter mais motivos a comemorar do que a lamentar. Em nosso campo de atuação, a Atenção Primária à Saúde e a Medicina de Família e Comunidade, alcançaram-se resultados extraordinários nestas duas décadas assim como estão lançadas para o futuro bases para o seu completo desenvolvimento. A ênfase dada à política de universalização com eqüidade irá requerer de todos os profissionais de saúde um crescente e renovado compromisso. Este esforço persistente deve ser destacado como um dos grandes ganhos em nossa sociedade que se reafirma e se aprimora como democrática. Por outro lado isto não se realizará sem esforços e investimentos em ciência e tecnologia, formação de recursos humanos e melhoria da qualidade em todas as suas dimensões, técnica e administrativa. Não se pode ignorar porém que resultados serão alcançados a médio e longo prazos e que só o trabalho árduo e permanente é capaz de realizar as mudanças almejadas. Assim a continuidade das políticas de Estado como a municipalização, a estabilidade dos mecanismos de financiamento, a capacitação de recursos humanos, a prioridade às ações de promoção e prevenção são aquelas que resultarão em melhores resultados em termos de indicadores de saúde. Não há como negar que algumas das questões consideradas problemáticas para o cidadão e usuário do Sistema Único de Saúde retornam diversas vezes à pauta sem que haja uma clareza de como serão resolvidas pelos gestores, profissionais e a sociedade brasileira em geral. É importante persistir com a decisão política por um modelo que garanta a territorialidade da assistência e que esta seja contínua, com vínculo, tal como

  8. Caracterización de bacterias probióticas aisladas del intestino grueso de cerdos como alternativa al uso de antibióticos

    OpenAIRE

    Henry Jurado G.; Diana Aguirre F; Cristina Ramírez T.

    2009-01-01

    Objetivos. Caracterizar bacterias ácido lácticas probióticas aisladas del intestino grueso de cerdos adultos como posible alternativa al uso de antibióticos. Materiales y métodos. Se incluyeron muestras de intestino grueso de 20 cerdos adultos, para el aislamiento de bacterias lácticas (BAL). La caracterización de los parámetros bioquímicos-fisiológicos de las BAL contemplo la evaluación del pH del fermento, determinación de ácidos orgánicos por HPLC y detección de isómeros de ácido láctico. ...

  9. Detección de una mutación puntual en el gen receptor Ryanodina (Ryr 1 en cerdos criollos colombianos

    Directory of Open Access Journals (Sweden)

    Muñoz Flórez Jaime Eduardo

    2008-12-01

    Full Text Available El síndrome de estrés porcino (PSS es una enfermedad hereditaria monogénica recesiva relacionada con el gen receptor ryanodina (Ryr1. Utilizando PCR-SSCP y PCR-RFLP se tipificaron genéticamente 14 individuos de cerdos comerciales con el rasgo sindactilia (Casco de Mula-CM, 21 San Pedreños -SP y 100 Zungos- ZN. Las razas CM y SP tuvieron las mismas frecuencias alélicas (F(H = 0.79 y F(h = 0.21, mientras que en los cerdos ZN no se encontró el alelo recesivo (h. La heterocigosidad (He fue de 0.28% para los cerdos CM y 0.23% para los SP. La He para la muestra poblacional fue de 0.066.

  10. Durabilidad de morteros de cemento en contacto con purines de cerdo

    OpenAIRE

    Massana Guitart, Jordi

    2010-01-01

    En el medio agrícola-ganadero el cemento y sus derivados, morteros y hormigones, son materiales ampliamente utilizados. Su uso se extiende desde la parte estructural de las edificaciones a elementos tales como revestimiento de paredes, comederos, o pavimentos tanto continuos como discontinuos o balsas de almacenamiento de purines, de hormigón en masa o armado o incluso de fábrica de ladrillo con revestimiento de mortero. Todos estos elementos se encuentran en ambientes de diversa agresividad,...

  11. Producción Fúngica de Proteasas Inducidas con Pelo de Cerdo Fungal Production of Proteases Induced with Pig Hair

    Directory of Open Access Journals (Sweden)

    Imir R Vázquez

    2008-01-01

    Full Text Available Se evaluaron las condiciones de producción de proteasas fúngicas inducidas con pelo de cerdo usando dos sistemas de cultivo, el proceso de cultivo en estado sólido y el cultivo líquido sumergido. El medio de cultivo fue Czapek-Dox en ambos casos. La cinética fue monitoreada cada 24 horas durante un periodo de incubación de 120 horas. La actividad proteolítica fue evaluada por hidrólisis de azocaseína y la producción de la biomasa fue estimada por el método gravimétrico. Rhizopus oryzae fue el microorganismo capaz de utilizar el pelo de cerdo como única fuente de carbono-nitrógeno, produciendo en cultivo sumergido los mayores títulos de actividad queratinasa. Se determinó el efecto que tiene el pH, el pretratamiento del sustrato y la adición de diferentes niveles de material queratinoso sobre el crecimiento fúngico y la actividad enzimática. Los resultados permitieron obtener títulos de actividad proteolítica de 975 U/L.The production of new proteases induced with pig hair was evaluated using two systems, submerged culture and solid state culture. Culture medium was Czapek-Dox for both systems. Enzyme production kinetic was monitored each 24 hours during 120 hours. Protelytic activity was evaluated with the azocaseín method and biomass production was determined by the gravimetric method. Rhizopus oryzae was the microorganism with the capacity of using the pig hair as the single carbon-nitrogen source, producing in submerged fermentation the highest enzymatic titers. The effect of pH, the substrate pretreatment and addition of different levels of substrate on proteolyitic activity and biomass production were determined. Results permitted to obtain titers of proteolytic activity of 975 U/L.

  12. Bienestar Animal: Comportamiento productivo y salud de las crías de cerdos alojadas en tecnología Flat Deck - Animal Well-being: Productive behaviour and health of the breeding of pigs housed in technology Flat Deck

    OpenAIRE

    Maikel Díaz Gutierrez; Yunier Hernández Cruz

    2010-01-01

    ResumenCon el objetivo de evaluar comportamiento productivo y de salud de las crías de cerdos alojados en tecnología Flat Deck se realiza el trabajo Bienestar Animal: Comportamiento productivo y de salud de las crías de cerdos alojadas en tecnología Flat Deck. Para ello se utilizaron 24 bóxer de maternidad de tecnología Flat Deck, de estos 12 de origen chino y 12 de origen español con características similares. Se analizaron un total de 1433 crías mestizas (cruce de hembras Yorkland entre la ...

  13. Estudio de los procesos autofágicos en la maduración de la carne de cerdo. Búsqueda de marcadores de bienestar animal y su efecto sobre la calidad del producto final

    OpenAIRE

    Potes Ochoa, Yaiza

    2013-01-01

    Los productos cárnicos y en concreto la carne de cerdo son uno de los principales sustentos alimenticios en los hogares europeos. Sin embargo este sustento se puede ver afectado por la alta mortalidad y/o alteración del producto que provoca el estrés en estos animales. En este proyecto se utilizaron 12 cerdos macho de cruce comercial (negativos al gen halotano), de los cuales 6 fueron expuestos a situaciones de estrés. Los animales se criaron en una granja experimental desde el destete y h...

  14. Comparación del cerdo criollo vs mejorado en la capacidad de digestión y fermentación de dietas con diferentes tipos de materias primas fibrosas

    OpenAIRE

    2008-01-01

    El presente trabajo tuvo como objetivo determinar la habilidad de los cerdos criollos colombianos en la capacidad de digerir y fermentar los nutrientes en dietas con altos niveles de fibra en comparación con los cerdos de razas mejoradas y utilizando cuatro diferentes fuentes de fibra: Micelio Seco, Torta de Palmiste, Cascarilla de Café y Cascarilla de Algodón. Para la cual se realizaron pruebas de digestibilidad fecal aparente a los diferentes nutrientes. De manera paralela se utilizo la téc...

  15. Detección de tetraciclinas en nueve lotes de alimentos para cerdos, tilapias y pollos producidos en Costa Rica: incumplimiento de normativas y disconformidades con el etiquetado oficial de garantía

    OpenAIRE

    Gutiérrez, Karla; Alfaro, Margarita; Granados, Fabio; Sánchez, Jorge; GARCÍA, Fernando; Rodríguez, César

    2010-01-01

    Sobre criterios de riesgos ambientales y sanitarios asociados al uso de antibióticos en la producción animal, se utilizó un método microbiológico y un método analítico por HPLC para cuantificar tetraciclinas y determinar su identidad, respectivamente, en lotes de alimento para cerdos (n=4), tilapia (n=3) y pollos de engorde (n=2) producidos en Costa Rica. Tres alimentos para consumo de cerdos y 2 alimentos para consumo de tilapias dieron resultados positivos por ambas técnicas. Los alimentos ...

  16. El azar y sus problemas

    OpenAIRE

    Fernández, Santiago

    2006-01-01

    Estamos en la Francia del siglo XVII. Su sociedad gira en torno a una vigorosa monarquía. Allí viven grandes pensadores y literatos: Descartes, Fermat, Pascal, Moliere, Racine, etc. Los juegos de dados, cartas y tableros con fichas son los entretenimientos más frecuentes. Pero, los juegos, cada vez más complicados, y las apuestas cada vez más elevadas crean la necesidad de calcular sus probabilidades de manera racional.

  17. Valoración de parámetros clínicos y lesiones en órganos de cerdos durante el período posdestete

    Directory of Open Access Journals (Sweden)

    Julián Mejía-Medina

    2012-01-01

    Full Text Available El destete de cerdos está asociado con múltiples factores que generan respuestas inflamatorias tempranas en órganos internos y alta incidencia de diarreas. En el Centro de Investigación San Pablo de la Universidad Nacional de Colombia sede Medellín, se evaluaron los parámetros clínicos y las lesiones en órganos internos en dieciséis cerdos destetados a veintiún días de edad, que fueron alimentados durante diez días con una dieta a base de leche. Cada uno, cinco, siete y diez días posdestete, se sacrificaron cuatro cerdos y se tomaron para estudio muestras de intestino delgado, estómago, hígado, páncreas, corazón, pulmones, riñones y bazo. Las lesiones determinadas fueron congestión, edema, y hemorragia; se asignó un valor según el grado de presentación: ausente (0, leve (1, leve-moderada (2, moderada-severa (3, severa (4. Los animales fueron pesados al destete y en el momento de sacrificio. Se encontraron diferencias (P < 0.01 en la aparición macroscópica de lesiones, peso de órganos, temperatura rectal y ganancia de peso. Los mayores valores se encontraron en el día uno posdestete y los menores en el día cinco; no obstante, para el día diez posdestete se observó una recuperación de las lesiones. La variable ocurrencia de diarreas presentó un comportamiento posdestete diferente y tendió a disminuir (P < 0.01.

  18. Valoración de parámetros clínicos y lesiones en órganos de cerdos durante el período posdestete

    Directory of Open Access Journals (Sweden)

    Rincón Ruiz Juan

    2012-03-01

    Full Text Available El destete de cerdos estã asociado con multiples factores que generan respuestas infiamatorias tem- pranas en órganos internos y alta incidencia de diarreas. En el Centro de Investigación San Pablo de la Universidad Nacional de Colombia sede Medellín, se evaluaron los parámetros clínicos y las lesiones en órganos internos en dieciséis cerdos destetados a veintiún días de edad, que fueron alimentados durante diez días con una dieta a base de leche. Cada uno, cinco, siete y diez días posdestete, se sa- crificaron cuatro cerdos y se tomaron para estudio muestras de intestino delgado, est%mago, hIgado, páncreas, corazón, pulmones, riñones y bazo. Las lesiones determinadas fueron congestión, edema, y hemorragia; se asignó un valor según el grado de presentación: ausente (0, leve (1, leve-moderada (2, moderada+severa 53<, severa 5=<. >os animales fueron pesados al destete y en el momento de sacrificio. Se encontraron diferencias (P < 0.01 en la aparición macroscópica de lesiones, peso de órganos, tem- peratura rectal y ganancia de peso. Los mayores valores se encontraron en el día uno posdestete y los menores en el día cinco; no obstante, para el día diez posdestete se observó una recuperación de las lesiones. La variable ocurrencia de diarreas presentó un comportamiento posdestete diferente y tendió a disminuir (P < 0.01.

  19. Evaluación de tres dosis de un preparado microbiano, obtenido en Ecuador, en la respuesta productiva y sanitaria de cerdos en posdestete

    Directory of Open Access Journals (Sweden)

    Luis Gerardo Flores-Mancheno

    2015-07-01

    Full Text Available Para evaluar tres dosis de un preparado microbiano obtenido en el Ecuador (4x106 UFC de bacterias lácticas, 1,5x105 UFC de levaduras por cada mililitro, en el comportamiento productivo y de salud en cerdos durante la etapa post-destete, se aplicó un diseño completamente aleatorizado, con cuatro tratamientos y cuatro repeticiones por tratamiento: T1 concentrado, T2, T3, T4 (concentrado más 5 mL/kg de peso vivo (PV, 10 mL/kg PV 15 mL/kg PV del preparado microbiano, respectivamente. Se utilizaron 160 cerdos castrados del cruce Landrace x Large White con Blanco Belga x Pietrain, de 28 d de edad y con 6,99 kg de PV. El mayor peso vivo final y la mejor ganancia de peso total y diaria (P < 0,0001 se obtuvo en el grupo de cerdos donde se agregó 15 mL/kg PV del preparado microbiano, con valores de 25,78 kg, 18,78 kg y 447,25 g, respectivamente. En el mismo grupo experimental se encontraron las conversiones de materia seca, proteína bruta y energía metabolizable más eficientes 1,54 kg /kg PV y aumento de peso vivo de 383,92 g/kg PV y 23,16 MJ/kg PV, respectivamente. También en este grupo se registró la menor presencia de animales con diarrea (P < 0,0001 6,85%. Se concluye que la adición de 15 mL/kg PV mejora el comportamiento productivo y sanitario de cerditos destetados.

  20. Desarrollo de un atlas interactivo de la histología del cerdo, como soporte en el aprendizaje de la morfología animal

    OpenAIRE

    2015-01-01

    1. Marco teórico.-- 2. Metodología.-- 3. Discusión, análisis y validación de los resultados.-- 4. Propuesta.-- 5. Conclusiones y Recomendaciones.-- La presente investigación tuvo como objetivo desarrollar un atlas interactivo de la histología del cerdo, como soporte en el aprendizaje de la morfología animal y la caracterización celular de los tejidos, se encuentra conformado por doce capítulos Tejido epitelial, conectivo, muscular, genital del macho, genital de la hembra, respiratorio, san...

  1. Mycoplasma hyopneumoniae y su relación con los procesos respiratorios del cerdo (Mycoplasma hyopneumoniae and its relation with the respiratory processes in swine

    Directory of Open Access Journals (Sweden)

    Dra. Evelyn Lobo

    2005-07-01

    Full Text Available Dentro de los problemas infecciosos más graves que afectan a la porcicultura intensiva en todo el mundo se encuentran los trastornos respiratorios, los cuales producen efectos adversos sobre la ganancia de peso, la eficiencia alimenticia y la mortalidad. Las enfermedades respiratorias en el cerdo son muy comunes y distribuidas en todos los países y climas donde existe producción porcina intensiva con valores de prevalencia que pueden llegar hasta el 100%. Existen múltiples micoorganismos que pueden afectar al aparato respiratorio del cerdo pero hoy día se acepta que todo proceso respiratorio es el resultado de una compleja interacción en la que pueden verse involucrados gran número de agentes por lo que habitualmente se ha hablado de un "Complejo Respiratorio Porcino" para referirnos a este tipo de problemas pulmonares de etiología múltiple, no obstante, actualmente se maneja el término de “Suis-Ide Diseases” para aludir a la complejidad de las etiologías y cuadros que intervienen en el tracto respiratorio del cerdo. Con relación a los agentes de naturaleza infecciosa se relacionan a los virus, las bacterias y los micoplasmas. En tal sentido algunos autores han señalado al virus del Síndrome Respiratorio y Reproductivo Porcino (PRRS y la Neumonía Enzoótica, cuyo agente etiológico principal es Mycoplasma hyopneumoniae, como los principales objetivos a tener en cuenta a la hora de plantear un programa vacunal. En el presente trabajo tiene como objetivo tratar algunos elementos que ayudaran a ampliar el conocimiento sobre Mycoplasma hyopneumoniae y su relación con los procesos respiratorios del cerdo a partir de la experiencia cubana en esta temática. Pretendiendo con este texto enriquecer algunos aspectos relacionados con este microorganismo y que a su vez sea útil para aquellos que se interesen en el tema.

  2. Mycoplasma hyopneumoniae y su relación con los procesos respiratorios del cerdo (Mycoplasma hyopneumoniae and its relation with the respiratory processes in swine)

    OpenAIRE

    Dra. Evelyn Lobo,

    2005-01-01

    Dentro de los problemas infecciosos más graves que afectan a la porcicultura intensiva en todo el mundo se encuentran los trastornos respiratorios, los cuales producen efectos adversos sobre la ganancia de peso, la eficiencia alimenticia y la mortalidad. Las enfermedades respiratorias en el cerdo son muy comunes y distribuidas en todos los países y climas donde existe producción porcina intensiva con valores de prevalencia que pueden llegar hasta el 100%. Existen múltiples micoorganismos que ...

  3. Eliminación de lawsonia intracellularis por cerdos de engorde en dos sistemas de producción en Yucatán, México

    OpenAIRE

    Jorge Carlos Rodríguez-Buenfil; Lourdes Rodríguez-Guzmán; José López-Espinoza; Mario Álvarez-Fleites; José C. Segura-Correa

    2008-01-01

    Se realizó un estudio longitudinal para determinar la frecuencia e incidencia de Lawsonia intracellularis en cerdos de engorde en una granja de múltiples sitios y otra de un sitio en Yucatán, México. Se utilizaron 59 y 58 lechones negativos a L. intracellularis de las granjas de múltiples sitios y un sitio, respectivamente, los cuales se monitorearon cada 14 días de la semana, 1 a la 21 de edad. La bacteria en heces se detectó utilizando la prueba de la reacción en cadena de la polimerasa. To...

  4. EVIDENCIA DE LA PRESENCIA DE ANTICUERPOS DE Mycoplasma hyopneumoniae EN CERDOS PROVENIENTES DE GRANJAS DE CRIANZA ARTESANAL DEL SUR DE LIMA.

    OpenAIRE

    Ibarra C., Marco; Practica privada.; Noé M., Norma; Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima; Alvarado S., Arnaldo; Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima. Apdo. 41-0068; Perales C., Rosa; Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima. Apdo. 41-0068

    2014-01-01

    Se realizó un estudio para determinar la presencia de anticuerpos de Mycoplasma hyopneumoniae en cerdos provenientes de granjas de crianza artesanal, mediante la detección de anticuerpos, utilizando el ensayo inmuno absorbente ligado a enzimas(ELISA). Ciento sesenta animales de 8 a 9 meses de edad procedentes de granjas de 4 distritos ubicados al Sur de Lima, fueron muestreados al momento de ser beneficiados en 2 carmales frigoríficos del valle de Lima. Se obtuvo una frecuencia de animales po...

  5. Eficiencia de depuración de purines de cerdo mediante humedales artificiales, con dos tiempos de retención hidráulica

    OpenAIRE

    González Ángel, Laura; Muñoz García, María Ángeles; Gómez Garrido, Melisa; Terrero, María Angélica; Faz Cano, Ángel

    2015-01-01

    [ESP] El sector agropecuario europeo y español ha experimentado una intensificación incrementando la presión global derivada de la sobreutilización y producción de nitrógeno. La normativa europea, refleja la necesidad de proteger las aguas de la contaminación causada por los nitratos Esta investigación tiene como objetivo determinar la eficiencia de la eliminación del nitrógeno y otros contaminantes en el purín de cerdo, a través de humedales artificiales, para dos tiempos de retención hid...

  6. Efecto del derivado de la lactosa sobre la población microbiana de muestras fecales de cerdos posdestete

    Directory of Open Access Journals (Sweden)

    Rosero Olga Lucía

    2006-03-01

    Full Text Available COMPENDIO Se estudió el efecto prebiótico del derivado de la lactosa (DL en la población microbiana presente en las muestras de heces (inóculo fecal de los cerdos posdestete. Se utilizó la técnica de producción de gas in vitro en 2 sustratos: el control pulpa de remolacha (PR y DL. Se desarrollaron dos etapas. En la primera se determinó la producción del gas y las proporciones de ácidos grasos volátiles (AGV, evaluando la actividad microbiana en los niveles (0, 0.2, 2, 20 y 100% de DL reemplazando a la PR, durante la fermentación de 144h. El tiempo medio de la producción del gas potencial se alcanzó a las 12h de la fermentación. Este tiempo se utilizó para la segunda etapa con tres niveles de DL (0, 20 y 100%. Se determinó el efecto del DL sobre la composición de la población microbiana (E. coli, lactobacillus y anaerobios facultativos totales, concentración de AGV y sobre el pH, como resultado de la fermentación bacteriana . Se incrementó la concentración del butirato (P < 0.001 y la acidificación (P < 0.001 del medio. El DL modificó la composición microbiana, que se reflejó en la disminución (P < 0.001 del número de colonias microbianas de E. coli. En conclusión el DL en niveles altos presentó efectos prebióticos. Palabras claves: Lactosa, prebiótico, fermentación in vitro, cerdo, butirato, Escherichia coli ABSTRACT Effect of Lactose Derivative (LD on the present microbial populationʼs in the sample faeces of weaned piglets The pre­biotic effect of Lactose Derivative (LD on the present microbial populationʼs in the sample faeces (faecal inocula of weaned piglets was studied . The in vitro gas-production technique was used. Using 2 substrates: sugarbeet pulp (SBP as control and LD as evaluation. Two stages were developed. In the first it the gas production and the Volatile Fatty Acids (VFA proportions, during 144h fermentation, to evaluate the effect of the microbial activity with different

  7. The biological traits of the large red scorpionfish, Scorpaena scrofa: Temporal and ontogenetic dynamics

    Science.gov (United States)

    Matić-Skoko, Sanja; Stagličić, Nika; Kraljević, Miro; Pallaoro, Armin; Dulčić, Jakov

    2015-01-01

    Large red scorpionfish, Scorpaena scrofa, is a prevalent, important and highly valued commercial species throughout the rocky coastal areas of the Mediterranean Sea. Despite this, there is a surprising scarcity of biological and ecological information for this species. As artisanal fisheries have a very long tradition in the whole Mediterranean, a considerable impact of continuous fishing pressure is expected on this valuable rockfish. To elucidate some biological parameters that indicate the status of S. scrofa in the Adriatic Sea and to show which consequences high fishery effort may have on its age, growth and reproduction, sampling was carried out in the middle Adriatic using trammel nets. Temporal trends in body size/weight of S. scrofa were also assessed using long-term data including specimens caught from 1960 to 2010. Temporal trends of S. scrofa in the middle Adriatic indicated significant decreases of 19% in length and 43% in total weight over time. In contrast, official landings for the last five years showed an increase of 13%. However, this increasing trend most likely reflects the continuous change in national policies for reporting the catches. Both catch data and landings statistics showed peak in catch per unit effort during summer which coincides with spawning period of S. scrofa. Length at first sexual maturity was observed at 29.0 cm for females and 24.9 cm for males. All specimens larger than 32 cm were mature. Age analysis revealed 15 age classes with a 25 year old female as the oldest specimen. However, age classes 3+ and 4+ were predominant in the total catch. The growth rate is relatively high during the first four years of life and afterwards it considerably slows down, with females growing at slightly slower rate and attaining slightly larger sizes than males. Given the identified biological implications that confirm our assumptions of inherent vulnerabilities and negative effects arising from continued artisanal fisheries practice

  8. Taxonomy Icon Data: pig [Taxonomy Icon

    Lifescience Database Archive (English)

    Full Text Available pig Sus scrofa domestica Chordata/Vertebrata/Mammalia/Theria/Eutheria/Artiodactyla Sus_scrofa_domestic...a_L.png Sus_scrofa_domestica_NL.png Sus_scrofa_domestica_S.png Sus_scrofa_domestica_NS.pn...g http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Sus+scrofa+domestica&t=L http://biosciencedbc.jp/taxonomy..._icon/icon.cgi?i=Sus+scrofa+domestica&t=NL http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Sus+scrofa+dom...estica&t=S http://biosciencedbc.jp/taxonomy_icon/icon.cgi?i=Sus+scrofa+domestica&t=

  9. Efecto de la Adición ex vivo del Lipopolisacárido de Escherichia coli sobre la Absorción de Lisina en Cerdos Destete Effect of Adding ex vivo Lipopolysaccharide from Escherichia coli on the Absorption of Lysine in Weaned Pigs

    Directory of Open Access Journals (Sweden)

    Cristian Gutiérrez Vergara

    2012-06-01

    Full Text Available Resumen. Con el destete brusco se induce la mortalidad de lactobacilos y el aumento de la población de E. coli, que liberan desde su pared productos proinflamatorios como el lipopolisacárido (LPS. El LPS activa una serie coordinada de señales que involucran a la familia de las MAPK, iniciando una cascada de reacciones que interrumpen los procesos de transporte de nutrientes y pueden progresar a fallo orgánico múltiple. El objetivo de este estudio fue determinar el efecto de la adición ex vivo de diferentes niveles de lipopolisacárido de E. coli sobre la absorción de lisina en cerdos destete. El trabajo de campo se realizó con 16 cerdos destetados a los 21 días de edad. Los animales fueron alimentados durante 10 días con una dieta basal que tuvo como componentes leche y algunos de sus derivados. Los cerdos se sacrificaron escalonadamente los días 1, 5, 7 y 10 posdestete y se realizó extracción del yeyuno, al que se le adicionaron las sustancias: Lisina 0,91 mg mL-1; LPS de E. coli (0,3 µg y dos inhibidores de la ruta MAPK (a SB-203580 (10, 20 y 50 mM (b U-0126 (10, 20 y 50 mM. La adición de 20 Mm de cualquiera de los inhibidores de MAPK aumentó significativamente la absorción de lisina (PAbstract. With the abrupt weaning mortality of lactobacillus is induced and E. coli population is increased, which release proinflammatory products from their wall cell such as lipopolysaccharide (LPS. LPS activates a coordinated series of signs that involve the MAPK family, initiating a cascade of reactions that interrupt nutrient transport processes and can progress to multiple organic failure. The aim of this study was to determine the effect of adding ex vivo different levels of lipopolysaccharide of E. coli on lysine absorption in weaned pigs. The experiment was carried out with 16 weaned pigs 21 days old. Animals were fed during 10 days with a basal diet with milk and some of its derivatives. Pigs were sequentially slaughtered on days one

  10. Characterization of Colombian creole pigs by RAMs Caracterización de cerdos criollos colombianos mediante la técnica molecular RAMs

    Directory of Open Access Journals (Sweden)

    Posso Andrés

    2006-12-01

    Full Text Available

    Using the molecular technique RAMs (Random Amplified Microsatellites, the genetic diversity and the existent genetic relationships were determined among 35 Colombian Creole pigs (Zungo, San Pedreño, Mule Foot and a sample of commercial type individuals. For ty six polymorphic loci were found and the heterozygocity values for the RAM markers fluctuated among 0.22 for CCA and 0.19 for CTand CGA, with 92.30% of polymorphic loci for CCA, 82.3 % for CT and 68.75% for CGA. The heterozygocity was of 0.2016 and the Fst was 0.3058 ±0.0433. The Nei distances tree established a very well defined group formed by the Zungo and San Pedreño breeds, which moved away from the rest of the individuals; the Mule foot pigs and Zungo (CLEM were grouped with the commercial ones, indicating introgression with foreign breeds.

    Key words: Swine; genetic variation; microsatellites; RAMs; Colombia.

    Mediante la técnica molecular RAMs (Random Amplified Microsatellites se determinó la diversidad y las relaciones genéticas en 35 cerdos criollos colombianos (Zungos, San Pedreño, Casco de Mula y en una muestra de animales de tipo comercial. Se encontraron 46 loci polimórficos; los valores de heterocigosidad por marcador RAMs fluctuaron entre 0.22 para CCA y 0.19 tanto para CT como para CGA, con 93.20% de loci polimórficos para CCA, 82.3% para CT y 68.75% para CGA. La heterocigosidad fue de 0.2016 y de 0.3058 ±0.0433 el Fst. El árbol de distancias de Nei definió bien las razas Zungo ySan Pedreño, las cuales se alejaron del resto de los individuos. Los cerdos Casco de Mula y Zungo (CLEM se agruparon con los comerciales, lo que indicó la presencia de introgresión con razas foráneas.

    Palabras claves: Cerdo; variación genética; microsatélites; RAMs; Colombia.

  11. El historiador y sus retos

    Directory of Open Access Journals (Sweden)

    Francisca López Civeira

    2014-01-01

    Full Text Available Para quienes ejercemos el oficio de historiadores constituye una labor cotidiana el manejo de diversas fuentes históricas como las bibliográficas, las publicaciones periódicas y, especialmente, los documentos de archivo a los que se les atribuye una función probatoria fundamental. Actualmente es notable la ampliación y diversidad de las fuentes para el trabajo histórico cuya validez es aceptada, entre las que han alcanzado un lugar de privilegio en tiempos recientes las orales, lo que implica la aplicación de técnicas adecuadas a su naturaleza. Sin embargo, ¿cuántas veces nos hemos preguntado cuáles son los fundamentos teóricos de este oficio? Sin dudas, la historia requiere del trabajo empírico para el estudio de un proceso, coyuntura o acontecimiento específico, es decir, la investigación histórica concreta tiene sus requerimientos en la indagación de lo factual, pero esto resulta insuficiente si no partimos de conocer las características y bases teóricas del área del saber a la que nos dedicamos.

  12. Utilización de gallinas enteras, crudas o cocidas en la alimentación de cerdos de levante-ceba

    Directory of Open Access Journals (Sweden)

    Reyes G.

    1990-12-01

    Full Text Available At the facilities of "El Verdal" poultry farm, located 1.6 miles away from Cali in the road to Jamundí, Valle del Cauca department, one research was conducted using 20 crossbred Yorkshire x Landrace pigs with 22 kg average initial weight, which were 127 days until they reached an average final weight of 86.4 kg. They were distributed at random in three treatments with different number of replications (one for the control and two for the experimental. The treatments were as follows: Boiled hens + Energy supplement (T1, Raw hens + Energy supplement (T2and Control or Commercial type feed according to phase (T . The main aim of this experiment was to look for the biological and economical feasibility of using whole hens (plus supplement comming from the normal daily mortality or discard, from a commercial poultry farm, as an alternative feeding source for growing finishing pigs. There were no statistical significant differences for daily weight gain, feed efficiency and feed consumption. There was a slight higher feed consumption (dry matter for T3 (2.14 kg, followed for TI and T2 (2.13 kg. In relation to daily weight gain, T had 515 g; TI' 507 g and T2, 497 g. The best feed efficiency was for T (4.16, followed for TI (4.2 and for T2 (4.28. Related to the economical results, higher feeding costs were for T ($ 52 167 and lower for TI and T2 ($ 42 167. Profits for fattening pig were better for TI ($ 15 415, followed for T2 ($ 14587 and for T3 ($ 8208.En las instalaciones de la granja avícola "El Verdal", localizada a un Km del perímetro urbano en la vía que de Cali conduce a Jamundí, Valle del Cauca se realizó un trabajo de investigación empleando 20 cerdos cruzados de las razas Yorkshire y Landrace, con un peso inicial promedio de 22 kg, los cuales fueron llevados hasta un peso final promedio de 86.4 kg a los 127 días y se distribuyeron al azar en tres tratamientos con diferente número de repeticiones (dos para los experimentales y una para

  13. Rendimiento de la canal y desarrollo de los órganos torácicos y abdominales de los 25 a los 45 kg en cerdos criollos pelones

    OpenAIRE

    Ronald Herve Santos Ricalde; Wilberth Trejo Lizama; Walter Osorto Hernández

    2011-01-01

    Se evaluó el crecimiento de la canal, de los cortes primarios y de los órganos torácicos y abdominales en 39 cerdos criollos pelones de Yucatán, México, con un peso vivo (pv) inicial de 6,9 ± 2,38 kg y sacrificados a diferentes pesos. Cuando alcanzaron el pv correspondiente para ser sacrificados (25; 30; 35; 40 y 45 kg) se les midió la grasa dorsal con un medidor de grasa dorsal, posteriormente se registró el peso y longitud de la canal y el porcentaje de la grasa dorsal a la altura de la déc...

  14. El cerdo. Historia de un elemento esencial de la cultura castellana en la conquista y colonización de América (siglo XVI

    Directory of Open Access Journals (Sweden)

    del Río Moreno, Justo L.

    1996-06-01

    Full Text Available Not available.

    El cerdo ibérico fue uno de los principales representantes de una cultura, la castellana, eminentemente ganadera. En la conquista y colonización de América tuvo un papel básico en la nutrición, siendo su adaptación al medio la principal garantía de supervivencia para soldados y colonos. Con el avance de la colonización, la crianza se vinculó al tributo indio, prosperando con tal prodigalidad que no tardó en perder protagonismo frente a otros ramos pecuarios con mayores expectativas comerciales, si bien los diferentes productos porcinos tuvieron siempre una gran aceptación en los mercados locales, especialmente en los indígenas.

  15. Estudio serológico preliminar de hepatitis E en cerdos en Chile Swine hepatitis E preliminary serological study in Chile

    Directory of Open Access Journals (Sweden)

    G. REINHARDT

    2003-12-01

    Full Text Available El virus de la hepatitis E (VHE en el hombre puede provocar epidemias masivas de hepatitis aguda. Se transmite vía oro-fecal, especialmente por agua contaminada. Se ha demostrado que el cerdo puede ser infectado con el VHE humano. Se han detectado anticuerpos y ARN del VHE en cerdos y otras especies animales, lo que sugiere un reservorio animal. Por otro lado, se identificó un agente nuevo denominado VHE porcino, distinto del VHE humano. En Chile, en seres humanos se ha encontrado prevalencia de anti- HEV de hasta 7%, pero no se ha determinado su estudio por RT-PCR ni tampoco se ha investigado su significado en especies animales. Mediante ELISA se determinaron anti-VHE (IgG, en 200 sueros de cerdos de planteles de la zona central de Chile y en 175 de la provincia de Valdivia, de diferentes edades y ambos sexos. De estos se seleccionaron 4 sueros anti-VHE positivos para estudios de VHE por RT-PCR, usando secuencias de VHE humano. Por ELISA se detectaron 9.5% anti-VHE positivos (19/200 en planteles de la zona central y 0.6% (1/175 de la provincia de Valdivia, Chile. El estudio por RT-PCR de los 4 sueros anti-VHE positivos no visualizó las bandas de precipitación esperadas. Estos resultados preliminares indican, por primera vez en Chile, que el virus E está presente en poblaciones de cerdos. Sin embargo, se necesitan de estudios más acabados para evaluar la magnitud del problema, usando secuencias de VHE porcinos y lograr la determinación del genoma viralThe hepatitis E virus (HEV can cause acute epidemic hepatitis outbreak in humans. The path of transmission is oro-fecal, especially through contaminated water.It has been demonstrated that pigs can be infected with the human form of HEV. HEV antibodies and RNA from HEV have been found in pigs and other animals. Recently a new strain, called porcine HEV, was identified which differs from the human HEV. In Chile prevalence of anti-HEV in human general population reach 7%. However no studies

  16. Modificación de biomarcadores histopatológicos e inmunohistoquímicos en las fibras musculares de cerdos tratados con clenbuterol

    Directory of Open Access Journals (Sweden)

    AM Molina

    2012-01-01

    Full Text Available El clenbuterol frecuentemente se ha utilizado de forma ilícita en el engorde del ganado, suponiendo ello un riesgo para la salud pública. La actual normativa prohíbe por tanto su uso en la cría del ganado. En este estudio se utilizaron 14 cerdos distribuidos al azar en dos grupos de estudio (n=7, uno control, y otro expuesto a 1 mg/kg de clenbuterol durante tres meses. Se utilizó el músculo longissimus lumbaris para la determinación de la concentración de clenbuterol, así como para la evaluación de los parámetros inmunohistoquímicos y el análisis estructural y ultraestructural de las fibras. El estudio de los distintos biomarcadores histopatológicos e inmunohistoquímicos de las fibras musculares, tras la exposición de los animales al clenbuterol, indicó la presencia de hipertrofia muscular, miofibrolisis y degeneración zenkeriana, observada tanto por microscopía óptica como electrónica. Mediante la técnica miosina ATP-asa se identificó un tipo de fibra denominada alterada en el grupo tratado que no apareció en el grupo control. Por tanto, se comprueba que el músculo longissimus lumbaris resulta una buena matriz para la investigación de la exposición a largo plazo a este compuesto en cerdos.

  17. Digestive Alkaline Proteases from Zosterisessor ophiocephalus, Raja clavata, and Scorpaena scrofa: Characteristics and Application in Chitin Extraction.

    Science.gov (United States)

    Nasri, Rim; Younes, Islem; Lassoued, Imen; Ghorbel, Sofiane; Ghorbel-Bellaaj, Olfa; Nasri, Moncef

    2011-01-01

    The aim of this work was to study some biochemical characteristics of crude alkaline protease extracts from the viscera of goby (Zosterisessor ophiocephalus), thornback ray (Raja clavata), and scorpionfish (Scorpaena scrofa), and to investigate their applications in the deproteinization of shrimp wastes. At least four caseinolytic proteases bands were observed in zymogram of each enzyme preparation. The optimum pH for enzymatic extracts activities of Z. ophiocephalus, R. clavata, and S. scrofa were 8.0-9.0, 8.0, and 10.0, respectively. Interestingly, all the enzyme preparations were highly stable over a wide range of pH from 6.0 to 11.0. The optimum temperatures for enzyme activity were 50°C for Z. ophiocephalus and R. clavata and 55°C for S. scrofa crude alkaline proteases. Proteolytic enzymes showed high stability towards non-ionic surfactants (5% Tween 20, Tween 80, and Triton X-100). In addition, crude proteases of S. scrofa, R. clavata, and Z. ophiocephalus were found to be highly stable towards oxidizing agents, retaining 100%, 70%, and 66%, respectively, of their initial activity after incubation for 1 h in the presence of 1% sodium perborate. They were, however, highly affected by the anionic surfactant SDS. The crude alkaline proteases were tested for the deproteinization of shrimp waste in the preparation of chitin. All proteases were found to be effective in the deproteinization of shrimp waste. The protein removals after 3 h of hydrolysis at 45°C with an enzyme/substrate ratio (E/S) of 10 were about 76%, 76%, and 80%, for Z. ophiocephalus, R. clavata, and S. scrofa crude proteases, respectively. These results suggest that enzymatic deproteinization of shrimp wastes by fish endogenous alkaline proteases could be applicable to the chitin production process.

  18. Evaluación del bienestar animal de cerdos en crecimiento-ceba alojados en sistema de cama profunda (Assessment of the animal welfare of growing-fattening pigs housed in deep bedding system

    Directory of Open Access Journals (Sweden)

    Cruz Martínez, Elizabeth

    2011-07-01

    Full Text Available ResumenSe evaluó el bienestar animal de cerdos en crecimiento-ceba al utilizar la tecnología de crianza en cama profunda comparado con el sistema tradicional sobre piso de concreto en el Instituto de Investigaciones Porcinas de Cuba.SummaryIt was evaluated the animal welfare of growing-fattening pigs using the deep bedding technology compared with the traditional system on the concrete floor in the Swine Research Institute of Cuba.

  19. Toxoplasma gondii Infection in Hunted Wild Boars (Sus scrofa): Heart Meat Juice as an Alternative Sample to Serum for the Detection of Antibodies.

    Science.gov (United States)

    Coelho, Catarina; Lopes, Ana Patrícia; Mesquita, João Rodrigo; Cardoso, Luís; Vieira-Pinto, Madalena

    2015-12-01

    Toxoplasmosis is a global zoonosis caused by the protozoan parasite Toxoplasma gondii. Detection of antibodies to T. gondii in serum samples from hunted animals may represent a key step for public health protection. It is also important to assess the circulation of this parasite in wild boar population. However, in hunted animals, collection of blood is not feasible and meat juice may represent an alternative sample. The purpose of the present study was to evaluate heart meat juice of hunted wild boars as an alternative sample for post-mortem detection of antibodies to T. gondii by modified agglutination test (MAT). The agreement beyond chance between results from meat juice assessed with Cohen's kappa coefficient revealed that the 1:20 meat juice dilution provided the highest agreement. McNemars's test further revealed 1:10 as the most suitable meat juice dilution, as the proportion of positive paired samples (serum and meat juice from the same animal) did not differ at this dilution. All together, these results suggest a reasonable accuracy of heart meat juice to detect antibodies to T. gondii by MAT and support it as an alternative sample in post-mortem analysis in hunted wild boars.

  20. Galanin is Co-Expressed with Substance P, Calbindin and Corticotropin-Releasing Factor (CRF) in The Enteric Nervous System of the Wild Boar (Sus scrofa) Small Intestine.

    Science.gov (United States)

    Czujkowska, A; Arciszewski, M B

    2016-04-01

    Galanin is a neuropeptide widely present in the enteric nervous system of numerous animal species and exhibiting neurotransmittery/neuromodulatory roles. Colocalization patterns of galanin with substance P (SP), corticotropin-releasing factor (CRF) and calbindin were studied in the small intestine of the wild boar using immunofluorescence technique. We demonstrated the presence of SP in substantial populations of galanin-immunoreactive (IR) submucous neurons. Additionally, different amounts of nerve fibres exhibiting simultaneous presence of galanin and SP were noted in the small intestinal smooth musculature, submucous ganglia, lamina muscularis mucosae and mucosa. In the wild boar duodenum, jejunum and ileum, the co-expression of galanin and calbindin was limited to minor populations of submucous neurons only. Single galanin-/CRF-IR nerve fibres were exclusively present in the duodenal and jejunal (but not ileal) mucosa. These results strongly suggest that galanin participates in neuronal control of the wild boar small intestine also by functional co-operation with other biologically active neuropeptides.

  1. The influence of environmental and physiological factors on the litter size of wild boar (Sus scrofa) in an agriculture dominated area in Germany.

    Science.gov (United States)

    Frauendorf, Magali; Gethöffer, Friederike; Siebert, Ursula; Keuling, Oliver

    2016-01-15

    The wild boar population has increased enormously in all of Europe over the last decades and caused problems like crop damage, transmission of diseases, and vehicle accidents. Therefore, it is necessary to investigate the underlying causes of this increase in order to be able to manage populations effectively. The purpose of this study was to analyse how environmental (food and climate) and physiological factors (maternal weight and age) as well as hunting and population density influence the litter size of wild boar populations in Northern Germany. The mean litter size in the studied population for the whole period was 6.6 (range 1–12), which is one of the highest in all of Europe. Litter size was positively influenced by maternal body weight, higher mast yield of oak as well as higher temperature in combination with higher precipitation in summer. Only higher temperature or only higher precipitation in summer however had a negative effect on litter size production. Probably,weather and food conditions act via maternal bodyweight on the litter size variation in wild boar. Hunting as well a s population density did not affect the litter size variation in this study which might indicate that wild boar population did not reach carrying capacity yet.

  2. How to survey classical swine fever in wild boar (Sus scrofa) after the completion of oral vaccination? Chasing away the ghost of infection at different spatial scales.

    Science.gov (United States)

    Saubusse, Thibault; Masson, Jean-Daniel; Le Dimma, Mireille; Abrial, David; Marcé, Clara; Martin-Schaller, Regine; Dupire, Anne; Le Potier, Marie-Frédérique; Rossi, Sophie

    2016-01-25

    Oral mass vaccination (OMV) is considered as an efficient strategy for controlling classical swine fever (CSF) in wild boar. After the completion of vaccination, the presence of antibodies in 6-12 month-old hunted wild boars was expected to reflect a recent CSF circulation. Nevertheless, antibodies could also correspond to the long-lasting of maternal antibodies. This paper relates an experience of surveillance which lasted 4 years after the completion of OMV in a formerly vaccinated area, in north-eastern France (2010-2014). First, we conducted a retrospective analysis of the serological data collected in 6-12 month-old hunted wild boars from 2010 up to 2013, using a spatial Bayesian model accounting for hunting data autocorrelation and heterogeneity. At the level of the whole area, seroprevalence in juvenile boars decreased from 28% in 2010-2011 down to 1% in 2012-2013, but remained locally high (above 5%). The model revealed the existence of one particular seroprevalence hot-spot where a longitudinal survey of marked animals was conducted in 2013-2014, for deciphering the origin of antibodies. Eleven out of 107 captured piglets were seropositive when 3-4 months-old, but their antibody titres progressively decreased until 6-7 months of age. These results suggest piglets were carrying maternal antibodies, few of them carrying maternal antibodies lasting until the hunting season. Our study shows that OMV may generate confusion in the CSF surveillance several years after the completion of vaccination. We recommend using quantitative serological tools, hunting data modelling and capture approaches for better interpreting serological results after vaccination completion. Surveillance perspectives are further discussed.

  3. Identification and differential expression of microRNAs in the ovaries of pigs (Sus scrofa) with high and low litter sizes.

    Science.gov (United States)

    Huang, L; Yin, Z J; Feng, Y F; Zhang, X D; Wu, T; Ding, Y Y; Ye, P F; Fu, K; Zhang, M Q

    2016-10-01

    Litter size affects profitability in the swine industry. Mammalian ovaries play important roles during reproduction, including ovulation and hormone secretion, which are tightly regulated by specific microRNAs (miRNAs). In this study, we investigated the effects of specific miRNAs on porcine litter size. We compared the ovarian miRNAs of Yorkshire pigs with high (YH) and low (YL) litter sizes using Solexa sequencing technology. We identified 327 and 320 miRNAs in the ovaries of YH and YL pigs respectively. A total of 297 miRNAs were co-expressed; 30 and 23 miRNAs respectively were specifically expressed in the two libraries. A total of 83 novel miRNAs were predicted; 37 specific miRNAs were obtained, of which 21 miRNAs were upregulated and 16 miRNAs were downregulated in YH compared with YL. Additionally, 19 628 and 19 250 target genes were predicted in the two libraries respectively. The results revealed that specific miRNAs (i.e., miR-224, miR-99a, let-7c, miR-181c, miR-214 and miR-21) may affect porcine litter size. The results of this study will help in gaining understanding of the role of miRNAs in porcine litter size regulation.

  4. Biologia i ecologia del senglar ("Sus scrofa" L., 1758) a dues poblacions del nordest ibèric. Aplicació a la gestió

    OpenAIRE

    Rosell Pagès, Carme

    1998-01-01

    El senglar és un dels mamífers més grans de Catalunya, és objecte d'una intensa activitat cinegètica i planteja importants reptes de gestió a causa de l'expansió demogràfica i geogràfica que manifesten les seves poblacions i pels problemes que comporten els danys a I'agricultura. Malgrat això no s'havia realitzat cap estudi aprofundit de la seva biologia en poblacions catalanes. En altres localitats de la Península Ibèrica es disposa en canvi de la informació elaborada en el marc de diverses ...

  5. Intraosseous Hydroxocobalamin in the Treatment of Acute, Severe Cyanide Induced Cardiotoxicity in a Swine (Sus Scrofa) Model - An Alternate Administration Route for Chemical Mass Casualties

    Science.gov (United States)

    2012-04-05

    methods – LICOX (PbO2), near infrared spectrophotometry (NIRS), and microdialysis. We will use three devices to assess neurologic function after...antidotal treatment. These measures are near infrared spectroscopy, brain oxygen tension, and brain microdialysis. Hypothesis We hypothesize...cerebral near infrared spectrometry (NIRS) oxygenation, and inflammatory markers. Methods 24 swine (48-52 kg) were intubated, anesthetized, and

  6. Annotation Of Novel And Conserved MicroRNA Genes In The Build 10 Sus scrofa Reference Genome And Determination Of Their Expression Levels In Ten Different Tissues

    DEFF Research Database (Denmark)

    Thomsen, Bo; Nielsen, Mathilde; Hedegaard, Jakob

    The DNA template used in the pig genome sequencing project was provided by a Duroc pig named TJ Tabasco. In an effort to annotate microRNA (miRNA) genes in the reference genome we have conducted deep sequencing to determine the miRNA transcriptomes in ten different tissues isolated from Pinky......, a genetically identical clone of TJ Tabasco. The purpose was to generate miRNA sequences that are highly homologous to the reference genome sequence, which along with computational prediction will improve confidence in the genomic annotation of miRNA genes. Based on homology searches of the sequence data...

  7. DNA extraction from skins of wild (Hydrochoerus hydrochaeris and Pecari tajacu) and domestic (Sus scrofa domestica) species using a novel protocol.

    Science.gov (United States)

    Ojeda, G N; Amavet, P S; Rueda, E C; Siroski, P A

    2012-03-19

    Sometimes, commercial products obtained from wild animals are sold as if they were from domestic animals and vice versa. At this point of the productive chain, legal control of possible wildlife products is difficult. Common in the commerce of northern Argentina, skins of two wild species, the carpincho and the collared peccary, look very similar to each other and to those of the domestic pig; it is extremely difficult to differentiate them after they have been tanned. Because there was no an adequate methodology to discriminate between leather of these three species, we developed a new methodology of DNA extraction from skin and leather. This new method involves digesting a leather sample using proteinase K, followed by precipitation of proteins with 5 M NaCl, cleaning with absolute isopropanol and DNA precipitation with 70% ethanol. DNA is hydrated in Tris-EDTA buffer. This protocol provided good-quality DNA suitable for analysis with molecular markers. This new protocol has potential for use in identifying leather products of these species using molecular markers based on RAPDs.

  8. Expression profiles of miRNA-122 and its target CAT1 in minipigs (Sus scrofa) fed a high-cholesterol diet

    DEFF Research Database (Denmark)

    Cirera Salicio, Susanna; Birck, Malene Muusfeldt; Busk, Peter Kamp

    2010-01-01

    The Göttingen minipig is an excellent model for studying effects of dietary high-fat intake on obesity. In this study, we analyzed the expression level of microRNA-122 (miRNA-122) and its target mRNA, CAT1, in intact young male minipigs fed either high-cholesterol or standard diet for 11 wk. Mi......RNA-122 and CAT1 are known to be important regulators of lipid metabolism. The weight of the young minipigs was monitored once a week during the feeding period; measurements of total cholesterol, triglycerides, high-density lipoproteins, and low-density lipoproteins were recorded at 4 time points (8, 14...... with a decrease in the expression of miRNA-122, confirming the implication of this microRNA in obesity. Gene expression levels of CAT1 did not differ between groups....

  9. Effects of a diet high in salt, fat, and sugar on telemetric blood pressure measurements in conscious, unrestrained adult Yucatan miniature swine (Sus scrofa).

    Science.gov (United States)

    Myrie, Semone B; McKnight, Leslie L; King, J Christopher; McGuire, John J; Van Vliet, Bruce N; Bertolo, Robert F

    2012-08-01

    Radiotelemetry was used to evaluate diet-related elevation of blood pressure in adult Yucatan miniature swine. Systolic arterial blood pressure (SAP), diastolic atrial blood pressure (DAP), heart rate, and locomotor activity were assessed in 9- or 11-mo-old Yucatan miniature pigs fed a standard diet or a North American-type diet high in salt, fat, and sugar (HSFS). Compared with pigs fed standard diet, pigs fed HSFS diet showed markedly elevated SAP (132 ± 3 compared with 156 ± 6 mm Hg), whereas DAP was unchanged (92 ± 2 compared with 99 ± 5 mm Hg). In addition, all pigs were modestly sensitive to short-term changes in dietary salt, as indicated by a 6% to 7% response in blood pressure parameters. According to these data, the increase in SAP for pigs on the HSFS diet was too large to be explained by the NaCl content of the diet alone. We found no evidence of endothelial dysfunction, and the relaxation responses of isolated coronary arteries actually were enhanced in the HSFS group. In conclusion, in a Yucatan miniature pigs model chronically fed a HSFS diet, DAP did not increase, but SAP and pulse pressure appeared to be affected by high dietary levels of fat or sugar (or both).

  10. Effects of a Taser: Conducted Energy Weapon on the Circulating Red-Blood-Cell Population and Other Factors in Sus scrofa

    Science.gov (United States)

    2013-03-30

    width, and hemoglobin) were measured with an automated hematology analyzer Micros 60 (Horiba ABX, Montpelier, France). Samples were refrigerated and...Koeller, U.S. Army, Attending Veterinarian , Bio-Effects Division, Human Effectiveness Directorate, 711th Human Performance Wing, U.S. Air Force Research...3. 21. Bell A. Morphologic evaluation of erythrocytes. In: Lotspeich- Steininger CA, Stiene-Martin EA, Koepke JA, editors. Clinical hematology

  11. A preliminary study of the effects of individual response to challenge tests and stress induced by humans on learning performance of weaned piglets (Sus scrofa).

    Science.gov (United States)

    Brajon, Sophie; Laforest, Jean-Paul; Schmitt, Océane; Devillers, Nicolas

    2016-08-01

    This study investigated whether individual behavioural characteristics of piglets and stress induced by experience with humans can influence learning performance. After weaning, piglets received a chronic experience with humans to modulate their emotional state: rough (ROU), gentle (GEN), or minimal (MIN) experience. Simultaneously, they were trained on a discrimination task. Afterward, their behaviour during challenge tests was assessed. The first learning step of the task involved associating a positive sound cue with a response (approach a trough) and success of piglets depended mostly on motivation to seek for reward. Although the experience with humans did not have direct effect, the degree of fear of handler, measured based on their reactivity to a human approach test, was related to motivation to seek rewards and learning speed of this first step in stressed ROU piglets, but not in MIN and GEN piglets. In contrast, the second learning step was more cognitively challenging, since it involved discrimination learning, including negative cues during which piglets had to learn to avoid the trough. Locomotion activity, measured during an open-field test, was associated with performance of the discrimination learning. To conclude, fearfulness towards humans and locomotion activity are linked with learning performance in relation to task complexity, highlighting the necessity to take into account these factors in animal research and management.

  12. Use of a line intercept snow track index and plot sampling for estimating densities of wild boar (Sus scrofa in southwestern Poland

    Directory of Open Access Journals (Sweden)

    B. Bobek

    2014-03-01

    Full Text Available n Bory Dolnośląskie Forest (southwestern Poland, in 8 forest districts with a total of 121,783 hectares (ha of forest area, 16 sampling plots of a total area of 7,100 ha were marked. Using the block count method, the presence of 157 wild boars was found in 12 sampling plots where the population density ranged from 2.2 to 52.9 individuals/1000 ha. By counting snow tracks along 16 line transects situated within the sampling plots (total length – 70.9 km, the presence of 103 wild boars was found. In places where tracks of wild boars were found, the relative population density of this species ranged from 0.10 to 5.6 individuals/km of transect. Using non-linear regression, a mathematical formula was derived where the relative population density index (N/km was the independent variable, and the absolute population density (N/1000 ha was the dependent variable. A high correlation was found between these variables (R2=0.85. It is suggested that the population numbers evaluated by snow tracks of wild boars found on line transects, and the regression equation derived in this study be used in the management of this species.

  13. Serological Survey of Porcine circovirus-2 in Captive Wild Boars (Sus scrofa) from Registered Farms of South and South-east Regions of Brazil.

    Science.gov (United States)

    Barbosa, C N; Martins, N R S; Freitas, T R P; Lobato, Z I P

    2016-04-01

    This study aimed to survey captive wild boars for antibodies against Porcine circovirus-2 (PCV-2) in registered farms. Serum samples (n = 1305) were collected from 90-day-old wild boars from 118 farms of the Brazilian South-east region, including the states of Minas Gerais and São Paulo, and South region, including the states of Paraná, Rio Grande do Sul and Santa Catarina. All herds (100%) presented reactive animals, in varying numbers and from low-to-high antibody titres, with the occurrence ranging from 82 to 89%. Considering farms, the average prevalence was of 84.9% (P < 0.05) and ranged from 54.1 to 94.95%. Regarding the geographic regions studied, the prevalence was of 100%, with PCV2 antibodies detected in wild boars of all regions. This study provides the first evidence of PCV2 antibodies in captive wild boars in Brazil.

  14. First Detection of Borrelia burgdorferi sensu lato DNA in Serum of the Wild Boar (Sus scrofa) in Northern Portugal by Nested-PCR.

    Science.gov (United States)

    Faria, Ana S; Paiva-Cardoso, Maria das Neves; Nunes, Mónica; Carreira, Teresa; Vale-Gonçalves, Hélia M; Veloso, Octávia; Coelho, Catarina; Cabral, João A; Vieira-Pinto, Madalena; Vieira, Maria L

    2015-03-01

    Lyme borreliosis is the most common tick-borne zoonosis in the northern hemisphere. Several vertebrates are crucial in the epidemiological cycle of Borrelia burgdorferi sensu lato, but the role of wild boar as a reservoir is still unknown. Sera were collected from 90 wild boars shot in the Trás-os-Montes region, Northern Portugal (hunting season 2011/2012). In this study, Borrelia DNA was detected for the first time by nested-PCR in three different sera, suggesting that the wild boar may be a potential reservoir for this spirochete. Sequencing results show 100% similarity with Borrelia afzelii. Further studies are needed to evaluate the public health risks associated with boar hunting.

  15. Isolation of Campylobacter from feral swine (Sus scrofa) on the ranch associated with the 2006 Escherichia coli O157:H7 spinach outbreak investigation in California

    Science.gov (United States)

    We report the isolation of Campylobacter species from the same population of feral swine that was investigated in San Benito County, California during the 2006 spinach-related Escherichia coli O157:H7 outbreak. This is the first survey of Campylobacter in a free-ranging feral swine population in the...

  16. {sup 137}Cs concentration in meat of wild boars (Sus scrofa) in Croatia a decade and half after the Chernobyl accident

    Energy Technology Data Exchange (ETDEWEB)

    Vilic, M. [Department of Physiology and Radiobiology, The Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb (Croatia)]. E-mail: marinko.vilic@vef.hr; Barisic, D. [Laboratory for Radioecology, Centre for Marine and Environmental Research, Ruder Boskovic Institute, PO Box 160, Bijenicka 54, 10002 Zagreb (Croatia)]. E-mail: dbarisic@irb.hr; Kraljevic, P. [Department of Physiology and Radiobiology, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb (Croatia)]. E-mail: petar.kraljevic@vef.hr; Lulic, S. [Laboratory for Radioecology, Centre for Marine and Environmental Research, Ruder Boskovic Institute, PO Box 160, Bijenicka 54, 10002 Zagreb (Croatia)]. E-mail: lulic@irb.hr

    2005-07-01

    Gamma-spectrometric measurements of {sup 137}Cs activities in meat of wild boars collected in Croatia at several locations with different levels of {sup 137}Cs contamination are presented. Samples were collected during the period between 2000 and 2002, about 15 years after the Chernobyl accident. {sup 137}Cs concentrations ranged over three orders of magnitude: 0.4-611.5 Bq kg{sup -1}. On the basis of these results, {sup 137}Cs concentrations at researched areas could be categorized into three groups: (i) the area of Slavonski Brod, Lipik and Slunj with {sup 137}Cs concentrations in meat of only a few Bq kg{sup -1}; (ii) the area of Vrbovsko and Sirac with {sup 137}Cs concentrations of a few tens of Bq kg{sup -1}; and (iii) the Fuzine area with {sup 137}Cs values in wild boar meat of a few hundreds of Bq kg{sup -1}. In areas with approximately equal contamination level, {sup 137}Cs concentrations in wild boar meat varied over two orders of magnitude. This fact suggests that the main reason for high {sup 137}Cs values in wild boar meat could be due to food consumed by wild boars, and only secondarily in contamination level of area where they live. Intensive mushroom consumption during autumn months could be one of the factors responsible for high {sup 137}Cs values in wild boar meat. An average dose arising from {sup 137}Cs due to ingestion of wild boar meat in Croatia is below radiological health concern except in the area of Fuzine, and only in cases of high annual wild boar meat intake, probably by hunters or members of their families.

  17. A Review of the Current Status of Relevant Zoonotic Pathogens in Wild Swine (Sus scrofa) Populations: Changes Modulating the Risk of Transmission to Humans.

    Science.gov (United States)

    Ruiz-Fons, F

    2017-02-01

    Many wild swine populations in different parts of the World have experienced an unprecedented demographic explosion that may result in increased exposure of humans to wild swine zoonotic pathogens. Interactions between humans and wild swine leading to pathogen transmission could come from different ways, being hunters and game professionals the most exposed to acquiring infections from wild swine. However, increasing human settlements in semi-natural areas, outdoor activities, socio-economic changes and food habits may increase the rate of exposure to wild swine zoonotic pathogens and to potentially emerging pathogens from wild swine. Frequent and increasing contact rate between humans and wild swine points to an increasing chance of zoonotic pathogens arising from wild swine to be transmitted to humans. Whether this frequent contact could lead to new zoonotic pathogens emerging from wild swine to cause human epidemics or emerging disease outbreaks is difficult to predict, and assessment should be based on thorough epidemiologic surveillance. Additionally, several gaps in knowledge on wild swine global population dynamics trends and wild swine-zoonotic pathogen interactions should be addressed to correctly assess the potential role of wild swine in the emergence of diseases in humans. In this work, viruses such as hepatitis E virus, Japanese encephalitis virus, Influenza virus and Nipah virus, and bacteria such as Salmonella spp., Shiga toxin-producing Escherichia coli, Campylobacter spp. and Leptospira spp. have been identified as the most prone to be transmitted from wild swine to humans on the basis of geographic spread in wild swine populations worldwide, pathogen circulation rates in wild swine populations, wild swine population trends in endemic areas, susceptibility of humans to infection, transmissibility from wild swine to humans and existing evidence of wild swine-human transmission events.

  18. Origin and distribution of the sciatic nerve in pig fetuses (Sus scrofa domesticus – Linnaeus, 1758 from the lineage Pen Ar Lan

    Directory of Open Access Journals (Sweden)

    Lázaro Antônio dos Santos

    2013-03-01

    Full Text Available The origin and distribution of the sciatic nerve was studied in 31 pig fetuses from the lineage Pen Ar Lan, being 22 males and 9 females, after being fixed in a 10% formaldehyde aqueous solution. In these animals, the lumbar vertebrae ranged from 5 to 7, with a predominance of 6, something which contributed to variations in the origin of this nerve. The sciatic nerve originated in 80.64% of the antimeres from the ventral branch of the fifth lumbar spinal nerve (L5; in 96.77 % of L6; in 6.45% of L7; in 100% of S1; and in 64.51% of S2. The composition of this nerve took place in 45.16% of the animals due to the union of L5, L6, S1 and S2; in 32.25% of L5, L6 and S1; in 12.90% of L6, S1 and S2; in 6.45% of L6, L7 and S1; and in 3.22% L5, S1 and S2. There was symmetry in the origin of the sciatic nerve in all specimens under study. This nerve sent branches to the superficial gluteal, semitendinosus, semimembranosus and biceps femoris muscles in all specimens, and, in a lesser frequency, to the twins, quadratus femoris, adductors, as well as the middle, accessory, and deep gluteal muscles. In 74.19% of the sample, a branch communicating to the pudendal nerve was observed.

  19. The Influence of Antral Ulcers on Intramural Gastric Nerve Projections Supplying the Pyloric Sphincter in the Pig (Sus scrofa domestica-Neuronal Tracing Studies.

    Directory of Open Access Journals (Sweden)

    Michal Zalecki

    Full Text Available Gastric ulcerations in the region of antrum pylori represent a serious medical problem in humans and animals. Such localization of ulcers can influence the intrinsic descending nerve supply to the pyloric sphincter. The pyloric function is precisely regulated by intrinsic and extrinsic nerves. Impaired neural regulation could result in pyloric sphincter dysfunction and gastric emptying malfunction. The aim of the study was to determine the effect of gastric antral ulcerations on the density and distribution of intramural gastric descending neurons supplying the pyloric sphincter in pigs.The experiment was performed on 2 groups of pigs: healthy gilts (n=6 and gilts with experimentally induced peptic ulcers in the region of antrum pylori (n=6. Gastric neurons supplying pyloric sphincter were labeled using the retrograde neuronal tracing technique (20μl of Fast Blue tracer injected into the pyloric sphincter muscle. After a week survival period the animals were sacrificed and the stomachs were collected. Then, the stomach wall was cross-cut into 0.5cm thick sections taken in specified intervals (section I - 1.5cm; section II - 3.5cm; section III - 5.5cm; section IV - 7.5cm starting from the sphincter. Consecutive microscopic slices prepared from each section were analyzed under fluorescent microscope to count traced neurons. Obtained data were statistically analyzed. The total number of FB-positive perikarya observed within all studied sections significantly decreased from 903.3 ± 130.7 in control to 243.8 ± 67.3 in experimental animals. In healthy pigs 76.1 ± 6.7% of labeled neurons were observed within the section I, 23.53 ± 6.5% in section II and only occasional cells in section III. In experimental animals, as many as 93.8 ± 2.1% of labeled cells were observed within the section I and only 6.2 ± 2.2% in section II, while section III was devoid of such neurons. There were no traced perikarya in section IV observed in both groups of pigs.Obtained results revealed for the first time significant impact of antral ulcerations on intramural descending nerve pathways supplying the pyloric sphincter in pigs, animals of increasing value in biomedical research and great economic importance.

  20. Effect of cattle on Salmonella carriage, diversity and antimicrobial resistance in free-ranging wild boar (Sus scrofa) in northeastern Spain.

    Science.gov (United States)

    Navarro-Gonzalez, Nora; Mentaberre, Gregorio; Porrero, Concepción M; Serrano, Emmanuel; Mateos, Ana; López-Martín, José M; Lavín, Santiago; Domínguez, Lucas

    2012-01-01

    Salmonella is distributed worldwide and is a pathogen of economic and public health importance. As a multi-host pathogen with a long environmental persistence, it is a suitable model for the study of wildlife-livestock interactions. In this work, we aim to explore the spill-over of Salmonella between free-ranging wild boar and livestock in a protected natural area in NE Spain and the presence of antimicrobial resistance. Salmonella prevalence, serotypes and diversity were compared between wild boars, sympatric cattle and wild boars from cattle-free areas. The effect of age, sex, cattle presence and cattle herd size on Salmonella probability of infection in wild boars was explored by means of Generalized Linear Models and a model selection based on the Akaike's Information Criterion. Prevalence was higher in wild boars co-habiting with cattle (35.67%, CI 95% 28.19-43.70) than in wild boar from cattle-free areas (17.54%, CI 95% 8.74-29.91). Probability of a wild boar being a Salmonella carrier increased with cattle herd size but decreased with the host age. Serotypes Meleagridis, Anatum and Othmarschen were isolated concurrently from cattle and sympatric wild boars. Apart from serotypes shared with cattle, wild boars appear to have their own serotypes, which are also found in wild boars from cattle-free areas (Enteritidis, Mikawasima, 4:b:- and 35:r:z35). Serotype richness (diversity) was higher in wild boars co-habiting with cattle, but evenness was not altered by the introduction of serotypes from cattle. The finding of a S. Mbandaka strain resistant to sulfamethoxazole, streptomycin and chloramphenicol and a S. Enteritidis strain resistant to ciprofloxacin and nalidixic acid in wild boars is cause for public health concern.

  1. Effect of cattle on Salmonella carriage, diversity and antimicrobial resistance in free-ranging wild boar (Sus scrofa in northeastern Spain.

    Directory of Open Access Journals (Sweden)

    Nora Navarro-Gonzalez

    Full Text Available Salmonella is distributed worldwide and is a pathogen of economic and public health importance. As a multi-host pathogen with a long environmental persistence, it is a suitable model for the study of wildlife-livestock interactions. In this work, we aim to explore the spill-over of Salmonella between free-ranging wild boar and livestock in a protected natural area in NE Spain and the presence of antimicrobial resistance. Salmonella prevalence, serotypes and diversity were compared between wild boars, sympatric cattle and wild boars from cattle-free areas. The effect of age, sex, cattle presence and cattle herd size on Salmonella probability of infection in wild boars was explored by means of Generalized Linear Models and a model selection based on the Akaike's Information Criterion. Prevalence was higher in wild boars co-habiting with cattle (35.67%, CI 95% 28.19-43.70 than in wild boar from cattle-free areas (17.54%, CI 95% 8.74-29.91. Probability of a wild boar being a Salmonella carrier increased with cattle herd size but decreased with the host age. Serotypes Meleagridis, Anatum and Othmarschen were isolated concurrently from cattle and sympatric wild boars. Apart from serotypes shared with cattle, wild boars appear to have their own serotypes, which are also found in wild boars from cattle-free areas (Enteritidis, Mikawasima, 4:b:- and 35:r:z35. Serotype richness (diversity was higher in wild boars co-habiting with cattle, but evenness was not altered by the introduction of serotypes from cattle. The finding of a S. Mbandaka strain resistant to sulfamethoxazole, streptomycin and chloramphenicol and a S. Enteritidis strain resistant to ciprofloxacin and nalidixic acid in wild boars is cause for public health concern.

  2. The prey pathway: a regional history of cattle (Bos taurus) and pig (Sus scrofa) domestication in the northern Jordan Valley, Israel.

    Science.gov (United States)

    Marom, Nimrod; Bar-Oz, Guy

    2013-01-01

    The faunal assemblage from the 9(th)-8(th) millennium BP site at Sha'ar Hagolan, Israel, is used to study human interaction with wild suids and cattle in a time period just before the appearance of domesticated animals of these species in the Jordan Valley. Our results, based on demographic and osteometric data, indicate that full domestication of both cattle and suids occurred at the site during the 8(th) millennium. Importantly, domestication was preceded in both taxa by demographic and metric population parameters indicating severe overhunting. The possible role of overhunting in shaping the characteristics of domesticated animals and the social infrastructure to ownership of herds is then explored.

  3. Serological and molecular investigation of the prevalence of Aujeszky's disease in feral swine (Sus scrofa) in the subregions of the Pantanal wetland, Brazil.

    Science.gov (United States)

    Paes, Rita de Cássia da Silva; Fonseca, A A; Monteiro, L A R C; Jardim, G C; Piovezan, U; Herrera, H M; Mauro, R A; Vieira-da-Motta, O

    2013-08-30

    The feral swine (FS) originated from the domestic pig and is present throughout the Brazilian wetland plain (the Pantanal). Aujeszky's disease (AD) was first serologically confirmed in the state of Mato Grosso do Sul (MS) in 2001; however, there was no viral confirmation. The aim of this study was to investigate antibodies against-SuHV-1 in the sera of feral swine in the studied areas, detect SuHV-1 through PCR and classify the viral genome. Among the 218 animals sampled, 186 were analyzed by ELISA, resulting in 88 (47.3%) reactive samples. In the serum neutralization test (SN), 57/179 (31.8%) samples presented antibodies against the AD virus (SuHV-1). By nested PCR, 104 DNA samples were extracted for analysis and confirmed with amplification of a fragment of glycoprotein B (gB) in five samples. The SuHV-1 was detected in 12 samples by using primers for glycoprotein E (gE) and viral genome was classified as Type I by ul44 partial sequencing. The amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows indicate that the vertical transmission contribute to maintain SuHV-1 in a free-living feral swine population. The origin of AD in the feral swine populations of the Pantanal is unknown, however, the determination of viral latency, the vertical transmission of the antigen by the amplification of SuHV-1 glycoprotein fragments in the fetuses of seropositive sows and genome typing contribute to the elucidation of the epidemiology of this disease in the wetlands of MS, Brazil.

  4. Efficacy of Intravenous Cobinamide Versus Hydroxocobalamin or Control for Treatment of Severe Hydrogen Sulfide Toxicity in a Swine (Sus Scrofa) Model

    Science.gov (United States)

    2016-05-18

    been entered into our computer file. Please advise us (by phone or mail) that your presentation was given. At that time, we will need the date...Publications and Presentations Section will route the request form to clinical investigations. S02 ISG/JAC ( Ethics Review) and Public Affairs (S9 MOW/PA) for

  5. Tissue concentrations of sulfamethazine and tetracycline hydrochloride of swine (Sus scrofa domestica) as it relates to withdrawal methods for international export.

    Science.gov (United States)

    Mason, Sharon E; Wu, Huali; Yeatts, Jim E; Baynes, Ronald E

    2015-04-01

    The use of water medications is a common practice in the US swine industry to treat and prevent infections in swine herds with minimal labor and without risk of needle breakage. There are concerns that FDA-approved withdrawal times (WDT) may be inadequate for several water medications when exporting pork products to countries where MRLs (maximum residue limits) are lower than US tolerance levels. In this study, withdrawal intervals (WDI) were estimated for pigs when dosed with tetracycline and sulfamethazine in water. The WDI were calculated using the FDA tolerance method (TLM) and a population-based pharmacokinetic method (PopPK). The estimated WDIs (14-16 days using TLM) were similar to the approved WDT of 15 days for sulfamethazine. However, the PopPK method extended WDIs for both sulfamethazine (19-20 days) and tetracycline (12 days) compared to the currently approved WDTs in the U.S. This study also identified potential differences in WDI between weanling and finisher pigs. In conclusion, the TLM may not always provide adequate WDT for foreign export markets especially when MRLs differ from tolerance levels approved for US markets. However, PopPK methods can provide conservative WDIs in situations with considerable variability in medication exposure such as with administration in water.

  6. Efecto de dos sistemas de simulación de monta durante la I. A. en el comportamiento reproductivo de las cerdas (Sus scrofa domestica)

    OpenAIRE

    2004-01-01

    Los estímulos del verraco alrededor de la Inseminación Artificial (IA) pueden afectar el desempeño reproductivo de la cerda. El propósito del presente estudio fue evaluar el efecto de alforjas de 25 kg sobre la grupa o un cinturón alrededor de la zona lumbar de las hembras simulando, respectivamente, la monta y presión de las extremidades delanteras del verraco durante la cópula, en el tiempo requerido para realizar la IA, tasa de parición y número de lechones nacidos vivos por cerda. Treinta...

  7. Quality of Cardiopulmonary Resuscitation When Directing the Area of maximal Compression by Transesophageal Echocardiography During Cardiac Arrest in Swine (Sus scrofa)

    Science.gov (United States)

    2013-12-30

    at 20mins CPP was measured over the duration of the experiment ROSC was defined as a regular cardiac rhythm with SBP >60 for 1min...69.2) 0 (0.00) No ROSC 4 (30.8) 13 ( 100 ) * * * p=0.032 p=0.002 pɘ.001 Figure 1. CPP at baseline, during VF arrest, BLS, and ACLS

  8. First report of a Trichinella papuae infection in a wild pig (Sus scrofa) from an Australian island in the Torres Strait region.

    Science.gov (United States)

    Cuttell, L; Cookson, B; Jackson, L A; Gray, C; Traub, R J

    2012-04-30

    Multiple Trichinella species are reported from the Australasian region although mainland Australia has never confirmed an indigenous case of Trichinella infection in humans or animals. Wildlife surveys in high-risk regions are essential to truly determine the presence or absence of Trichinella, but in mainland Australia are largely lacking. In this study, a survey was conducted in wild pigs from mainland Australia's Cape York Peninsula and Torres Strait region for the presence of Trichinella, given the proximity of a Trichinella papuae reservoir in nearby PNG. We report the detection of a Trichinella infection in a pig from an Australian island in the Torres Strait, a narrow waterway that separates the islands of New Guinea and continental Australia. The larvae were characterised as T. papuae (Kikori strain) by PCR and sequence analysis. No Trichinella parasites were found in any pigs from the Cape York Peninsula. These results highlight the link the Torres Strait may play in providing a passage for introduction of Trichinella parasites from the Australasian region to the Australian mainland.

  9. Positive effects of treatment of donor cells with aphidicolin on the preimplantation development of somatic cell nuclear transfer embryos in Chinese Bama mini-pig (Sus Scrofa).

    Science.gov (United States)

    Zhang, Ting-Yu; Dai, Jian-Jun; Wu, Cai-Feng; Gu, Xiao-Long; Liu, Liang; Wu, Zhi-Qiang; Xie, Yi-Ni; Wu, Bin; Chen, Hui-Lan; Li, Yao; Chen, Xue-Jin; Zhang, De-Fu

    2012-02-01

    To optimize somatic cell nuclear transfer (SCNT) procedures in mini-pigs, the present study was designed to examine the effects of donor cell types and aphidicolin (APC) treatment on in vitro development of reconstructed embryos. Oviduct epithelial cells (OEC), ear fibroblast cells (EFC) and cumulus cells (CC) derived from mini-pigs were treated with serum starvation only or serum starvation followed by treatment of 0.1 µg/mL APC. The reconstructed embryos were cultured for 7 days to evaluate their developmental competency. Cleavage and blastocyst formation rates of reconstructed embryos derived from the OEC by APC treatment were significantly higher than the serum starvation (61.82% vs. 56.25%, 24.55% vs. 17.86%; P cell types. Therefore, our results suggest that treatment of CC with serum starvation plus APC prior to nuclear transfer is more suitable in SCNT of mini-pigs.

  10. Intoxicação espontânea por Senna occidentalis em javalis (Sus scrofa ferus no estado de Goiás

    Directory of Open Access Journals (Sweden)

    Fabiano J.F. de Sant'Ana

    2011-08-01

    Full Text Available Descreve-se um surto de intoxicação por Senna occidentalis em javalis no Estado de Goiás. De um rebanho de 80 javalis, 15 adoeceram e um morreu. Os sinais clínicos observados foram apatia, prostração, ataxia, tremores musculares, incoordenação, relutância em mover-se, decúbito esternal ou lateral, paresia e paralisia espástica, principalmente dos membros pélvicos. As principais alterações macroscópicas consistiam de palidez moderada a acentuada da musculatura esquelética dos membros pélvicos e torácicos e no lombo, especialmente nos músculos da coxa. Microscopicamente, observou-se degeneração e necrose flocular, multifocal, leve a moderada, monofásica, com fragmentação de fibras na musculatura esquelética. Nos cortes transversais, havia fibras musculares tumefeitas e hipereosinofílicas. Adicionalmente, havia degeneração microvacuolar hepatocelular difusa, leve a moderada. A atividade sérica da CK estava acentuadamente elevada em dois javalis avaliados e da TGO aumentou em um javali afetado.

  11. The prey pathway: a regional history of cattle (Bos taurus and pig (Sus scrofa domestication in the northern Jordan Valley, Israel.

    Directory of Open Access Journals (Sweden)

    Nimrod Marom

    Full Text Available The faunal assemblage from the 9(th-8(th millennium BP site at Sha'ar Hagolan, Israel, is used to study human interaction with wild suids and cattle in a time period just before the appearance of domesticated animals of these species in the Jordan Valley. Our results, based on demographic and osteometric data, indicate that full domestication of both cattle and suids occurred at the site during the 8(th millennium. Importantly, domestication was preceded in both taxa by demographic and metric population parameters indicating severe overhunting. The possible role of overhunting in shaping the characteristics of domesticated animals and the social infrastructure to ownership of herds is then explored.

  12. Novel Y-chromosome short tandem repeats in Sus scrofa and their variation in European wild boar and domestic pig populations

    DEFF Research Database (Denmark)

    Iacolina, Laura; Brajkovic, Vladimir; Canu, Antonio;

    2016-01-01

    Y-chromosome markers are important tools for studying male-specific gene flow within and between populations, hybridization patterns and kinship. However, their use in non-human mammals is often hampered by the lack of Y-specific polymorphic markers. We identified new male-specific short tandem r...

  13. Identificación de fimbrias de Escherichiacoli enteropatógeno mediante inmunohistoquímica en cerdos lactantes con diarrea Inmunohistochemical identification of enteropathogenic E. coli fimbriae in suckling pigs with diarrhoe

    Directory of Open Access Journals (Sweden)

    A.M. CANAL

    1999-01-01

    Full Text Available El presente trabajo se realizó con el propósito de considerar a las técnicas inmunohistoquímicas como método de diagnóstico seguro y rápido para la identificación de antígenos fimbriales de E. coli en intestino delgado de cerdos lactantes con cuadro diarreico. Se realizó aislamiento microbiológico e inmunohistoquímica en duodeno, yeyuno e íleon de 50 cerdos lactantes infectados naturalmente. E. coli sola (34% así como asociada (24% a otros microorganismos fue el agente etiológico mas frecuentemente diagnosticado en cerdos lactantes con diarrea, seguido de Salmonella sp., Rotavirus y Campylobacter sp. Mediante IHQ se identificó antígenos fimbriales en 10 cerdos con aislamiento de E. coli, en 1 con Salmonella sp. y en 5 sin diagnóstico etiológico. El antígeno fimbrial más frecuentemente encontrado fue F41 en 8 cerdos entre 1 y 35 días de edad, seguido de la asociación de F4-F41 en 5 animales de 15 a 28 días y, finalmente, F4 en 3 cerdos entre 8 y 35 días. E. coli F4 fue identificado en los tres segmentos intestinales, mientras que E. coli F41 colonizó principalmente íleon y yeyuno. Los resultados de la presente investigación confirman la importancia de E. coli en los cuadros de diarrea en cerdos lactantes, así como la efectividad de las técnicas IHQ para la identificación de antígenos fimbriales en cortes de intestino embebidos en parafina. Sin embargo, se debe resaltar la posible presentación de otros factores de adhesión de E. coli a los enterocitos, lo que permite proyectar futuras investigacionesA study to evaluated the inmunohistochemistry techniques (IHC as dignosis the E. coli fimbrial antigens in intestines of sucklings pigs with diarrhea, was carried out. Fifty suckling pigs, naturally infected, with diarrhoea were analyzed to microbiologically isolate and identify the antigen fimbrial in the small intestine with Inmunohistochemistry (Indirect Inmunofluorencence and Streptavidin-Biotin-Peroxidase and

  14. CARACTERIZACIÓN DE BACTERIAS PROBIÓTICAS AISLADAS DEL INTESTINO GRUESO DE CERDOS COMO ALTERNATIVA AL USO DE ANTIBIÓTICOS

    Directory of Open Access Journals (Sweden)

    Henry Jurado G

    2009-08-01

    Full Text Available Objetivos. Caracterizar bacterias ácido lácticas probióticas aisladas del intestino grueso de cerdos adultos como posible alternativa al uso de antibióticos. Materiales y métodos. Se incluyeron muestras de intestino grueso de 20 cerdos adultos, para el aislamiento de bacterias lácticas (BAL. La caracterización de los parámetros bioquímicos-fisiológicos de las BAL contemplo la evaluación del pH del fermento, determinación de ácidos orgánicos por HPLC y detección de isómeros de ácido láctico. Los procedimientos microbiológicos evaluaron la capacidad de inhibición del crecimiento de patógenos y la actividad antagónica del probiótico vs antibiótico con su interacción competitiva. Se utilizaron pruebas API CHL 50 para la identificación de las BAL que se complementaron con la producción de gas, actividad de catalasa y viabilidad a diferentes concentraciones de sales biliares y temperaturas. Resultados. El plan de análisis permitió seleccionar dos aislados que se identificaron como: Lactobacillus plantarum 1 H1 y Lb. plantarum 1 H2. Las pruebas microbiológicas evidenciaron que las BAL seleccionadas inhibieron principalmente los enteropatógenos: Escherichia coli, Salmonella typhimurium y Clostridium perfringens. Las pruebas bioquímicas fueron compatibles con el género Lactobacillus, mostrando el siguiente perfil: Catalasa negativas; no productoras de gas; productoras de isómeros DL; resistentes a sales biliares y pH óptimo de crecimiento 3.5 a 38oC. Conclusiones. Los dos aislados de Lactobacillus plantarum 1 reunieron las características para el uso potencial como probióticos. Se recomienda su uso como complemento de terapias con antibióticos y en la elaboración de inóculos para aplicación en raciones alimenticias de lechones destetos, sin embargo, estudios complementarios se requieren para evaluar el efecto directo del probiótico como sustituto del uso de antibióticos in vivo.

  15. Dicty_cDB: Contig-U08982-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 365185. 44 6.6 1 ( EU333163 ) Sus scrofa breed Chinese northeast wildboar mitoc... 44 6.6 1 ( EU117375 ) Sus...F304203 ) Sus scrofa breed Swedish wild boar mitochondrion,... 44 6.6 1 ( AF30420...2 ) Sus scrofa breed Landrace mitochondrion, partial ... 44 6.6 1 ( AF304201 ) Sus scrofa breed Italian wild boar

  16. Contenidos de isómeros trans de los ácidos grasos en productos cárnicos, (II Tejido adiposo y grasa intramuscular del cerdo

    Directory of Open Access Journals (Sweden)

    Lluch, M. C.

    1993-04-01

    Full Text Available We give the results obtained for the determination of trans fatty acids in subcutaneous and intramuscular fats from different pork samples, using gas chromatography. Values obtained are low and similar in both kind of fats, with a mean value of 0.6% of C18:1 trans. A clear influence of animal feeding is detected due to the significant differences of this isomer content between samples from different origin. Also, correlations have been observed between C18:1 t. content and saturated, mono, polinsaturated fatty acids and fat intramuscular contents.

    Se presentan los resultados obtenidos en la determinación de ácidos grasos trans en una serie de muestras de tejidos subcutáneo y muscular, procedentes de canales de cerdo, mediante cromatografía en fase gaseosa. Ambos tipos de grasas presentan valores similares y relativamente bajos, con un valor medio del 0.6% de 018:1 t. Se detecta una marcada influencia de la alimentación, puesta de manifiesto por las diferencias significativas obtenidas para los contenidos de C18:1 trans, en función del origen de las muestras. Igualmente, se recogen las correlaciones obtenidas entre los contenidos de C18:1 t. y los de ácidos grasos saturados, monoinsaturados y poliinsaturados, así como con el contenido graso intramuscular.

  17. Neoliberalismo e intervencionismo: sus fuentes y razones

    Directory of Open Access Journals (Sweden)

    Salomón Kalmanovitz

    1998-08-01

    Full Text Available A partir de una reseña sobre el papel del intervencionismo estatal en la conducción de la economía y de la pugna con el neoliberalismo, se hace una revisión de los modelos económicos aplicados a lo largo del siglo y se muestran sus rasgos principales, sus problemas y sus paradojas. A partir de alfí, se examina lo sucedido en América Latina, para dar cuenta de las particularidades del caso colombiano, cuyo modelo en esta década muestra una distancia grande del modelo neoliberal, mientras sus problemas lo acercan a los dilemas y sin salidas del intervencionismo, en un contexto que requeriría otras fórmulas para hacer posible la paz. Frente a ello, se examinan las posiciones y propuestas de los economistas, vistas bajo la aguda polarización entre intervencionismo y neoliberalismo, pero en circunstancias críticas que alejan los logros en torno a la modernización, la estabilidad de precios, la sólida fundamentación macroeconómica y la democratización política.

  18. El grafeno y sus propiedades especiales

    Directory of Open Access Journals (Sweden)

    F. Guinea

    2011-03-01

    Full Text Available El grafeno, formado por una capa de átomos de carbono, es un nuevo material con interesantes propiedades, desde un punto de vista fundamental, y también por sus posibles aplicaciones. Se describen algunas de estas propiedades, y se discuten algunos avances recientes en la investigación en este material.

  19. Efecto de la fermentación microbiana en el intestino grueso sobre la digestión, absorción y utilización de nutrientes : comparación entre el cerdo landrace y el ibérico /

    OpenAIRE

    2004-01-01

    Descripció del recurs: el 13 de juliol de 2004 La presente tesis doctoral tiene como objetivos principales valorar el efecto del tipo de carbohidratos en el alimento sobre el engrasamiento de la canal, en dos razas porcinas: el cerdo ibérico y el landrace. En una primera fase experimental se utilizaron 48 cerdos (88,4 kg PV), 24 Ibéricos y 24 Landrace, a los que se administraron dos raciones que diferían en las principales fuentes de carbohidratos: maíz (Dieta MZ), altamente digestible, o ...

  20. Determinación de las características anatómicas de las arterias coronarias de cerdo mediante técnica de inyección con látex

    Directory of Open Access Journals (Sweden)

    Guillermo Rivera

    2015-06-01

    Full Text Available Objetivo: Determinar las características anatómicas de las arterias coronarias del cerdo, mediante inyección con látex. Materiales y métodos: Se usaron 100 corazones de cerdo. Se insertaron sondas Nelaton a través de las arterias coronarias y se les inyectó solución de látex y tinta china. Se determinó el diámetro y el punto de origen para la coronaria derecha, coronaria izquierda, interventricular paraconal y circunfleja. Se cuantificaron el número de ramas diagonales, marginales ventriculares izquierdas y postero laterales. Se determinó frecuencia de ramus intermedius y tipo de dominancia. Se realizó análisis estadístico con un error estándar del 5%, con el Epidat 3,1. Resultados: El 100% de los orígenes anatómicos de las arterias coronarias fueron normales. La dominancia mas frecuente fue la derecha, 73%. El promedio de número de ramas marginales izquierdas, diagonales y posterolaterales fue de 3. La frecuencia de ramus intermedius fue de 12%. El calculo de chi cuadrado de Pearson mostró significancia entre el ramus intermedius y número de ramas marginales izquierdas (p=0,0009. Conclusiones: Las características anatómicas de las arterias coronarias del cerdo son similares a las del humano. El ramus intermedius se comporta como una primera rama marginal izquierda y no como una primera rama diagonal como lo afirman algunos autores.

  1. Identificación de Actinobacillus pleuropneumoniae biotipo 1, serotipo 1, de pulmones de cerdo con y sin lesiones neumónicas utilizando la técnica de inmunohistoquímica

    OpenAIRE

    Rigoberto Hernández Castro; Gilberto Chávez Gris; José Ángel Gutiérrez Pabello

    2002-01-01

    En el presente estudio se comparó el aislamiento bacteriológico y una técnica de inmunohistoquímica con la variedad del complejo avidina-biotina-peroxidasa para la identificación de Actinobacillus pleuropneumnoniae biotipo 1, serotipo 1. Se utilizaron 100 pulmones de cerdos clínicamente sanos sacrificados en rastro, 50 con lesiones macroscópicas aparentes y 50 sin lesiones macroscópicas. Todas las muestras se analizaron mediante histopatología, bacteriología e inmunohistoquímica. Las lesiones...

  2. Influencia de la alimentación sobre los ácidos grasos, el colesterol y el retinol en distintos depósitos grasos del cerdo de raza celta

    OpenAIRE

    Dominguez Valencia, Ruben

    2012-01-01

    Se cebarán tres lotes de cerdos de raza Celta castrados (machos y hembras), hasta una edad de 16 meses. Al primero de ellos (lote A) se le suministrará pienso compuesto como único alimento. Al segundo (lote B) se le suministrará pienso compuesto hasta los 12 meses y una dieta mixta pienso/castaña durante los 4 meses previos al sacrificio. Al tercer lote (C) se le suministrará una dieta mixta pienso/castaña durante el mes 13, y únicamente castaña durante los tres meses previos al sacrificio. ...

  3. Selección de microorganismos indígenas mediante pruebas in vitro, para mejorar el estado sanitario y la performance de crecimiento durante la crianza intensiva de cerdos

    OpenAIRE

    Bertozzi, Ezequiel

    2012-01-01

    Los probióticos representan una de las alternativas en la producción porcina para disminuir el uso desmedido de antimicrobianos y reducir la presencia de patógenos bacterianos y xenobióticos de uso veterinario en los alimentos. El objetivo de este trabajo fue seleccionar un inóculo microbiano con capacidades probióticas mediante pruebas in vitro, integrado por bacterias indígenas de la microbiota intestinal de cerdos, para mejorar el estado sanitario y la performance de crecimiento durante su...

  4. Determinación de la presencia de Helicobacter pylori mediante un dispositivo cromatográfico específico, en cerdos sacrificados en el Frigorífico Vijagual.

    OpenAIRE

    Gonzalez Gomez, Ana; Rodriguez Suarez, Elena; Gomez Celis, Joaquin

    2005-01-01

    En las unidades de producción porcina se han presentado patologías de etiología incierta que indican la presencia de lesiones gástricas compatibles con infección por Helicobacter sp. Ante la necesidad de determinar la asociación entre los hallazgos clínicos y la infección con Helicobacter sp., el trabajo se propuso confirmar por serología la presencia de la bacteria. El desarrollo de la investigación utilizó una muestra de 100 cerdos de sacrificio del Frigorífico Vijagual, de la ciudad de Buc...

  5. Estudio clínico, anatomopatológico y de biología molecular de cuadros entéricos en cerdos de crecimiento y terminación

    OpenAIRE

    Pérez, Estefanía Marisol

    2016-01-01

    Se evaluó la participación de Lawsonia intracellularis, Brachyspira hyodysenteriae, Brachyspira pilosicoli y Salmonella spp. en el desarrollo de infecciones clínicas y subclínicas en cerdos de la etapa de engorde. Se realizó un estudio retrospectivo histopatológico de casos de archivo en el que se relacionaron las lesiones histopatológicas con los agentes etiológicos identificados por IHQ, WS y PCR a partir de tejidos embebidos en parafina. Se detectó asociación estadística entre las lesiones...

  6. Regionalismo sudamericano: sus características

    Directory of Open Access Journals (Sweden)

    Noemí B. Mellado

    2013-03-01

    Full Text Available En el espacio suramericano coexisten distintos procesos: a nivel subregional –CAN, MERCOSUR, ALBA- y regional –UNASUR, CELAC-; algunos de ellos son manifestación de un regionalismo "nuevo", "en construcción", "en transición", "posliberal", según los autores. Tienen como rasgo común y distintivo su diferente naturaleza, la amplitud temática y la no exclusividad de sus miembros. Es propósito de este trabajo describir al regionalismo actual en sus características principales y contribuir de algún modo al debate académico-científico sobre esta categoría de análisis, desde una perspectiva latinoamericana.

  7. Histological data concerning the pig (sus scrofa) for use in radiobiology. I. Normal histology of certain systems. 2. Injuries caused by total {gamma} irradiation; Donnees histologiques sur le porc (sus scrofa), utilisables en radiobiologie. 1. histologie normale de certains appareils. 2. etude des lesions consecutives a une irradiation {gamma} totale

    Energy Technology Data Exchange (ETDEWEB)

    Guenet, J.L.; Vaiman, M.; Nizza, P. [Commissariat a L' Energie Atomique, Fontenay-aux-Roses (France). Centre d' Etudes Nucleaires

    1966-06-01

    The domestic pig lately become a laboratory whose anatomical and physiological characteristics appear to be appreciated for certain radiological studies. In this report the authors describe research which they have carried out (in particular in the field of histology) on domestic pigs of different species with a view to compiling a record which might be of help to future workers in this field. The second part of their work deals with anatomical and histological injuries caused by total exposure to gamma radiation from cobalt 60 at doses of around 285 rads. (authors) [French] Le porc domestique est devenu depuis peu un animal de laboratoire, dont les caracteristiques anatomiques et physiologiques paraissent fort appreciees pour certaines etudes de radiobiologie. Dans ce rapport les auteurs font part des recherches qu'ils ont effectuees (notamment dans le domaine de l'histologie) sur des porcs domestiques de differentes races, de maniere a constituer un document capable de venir en aide aux utilisateurs eventuels. La deuxieme partie de leur travail traite de lesions anatomiques et histologiques, consecutives a des expositions totales aux rayonnements gamma du cobalt 60 a des doses voisines de 285 rads. (auteurs)

  8. Occurrence of Hymenoptera on Sus scrofa carcasses during summer and winter seasons in southeastern Brazil Ocorrência de Hymenoptera em carcaças de Sus scrofa durante as estações de inverno e verão do sudeste do Brasil

    Directory of Open Access Journals (Sweden)

    Leonardo Gomes

    2007-09-01

    Full Text Available Considerable importance has been given to nest construction and larval food transport to the nest as a precondition for the eusociality of insects. Most adult hymenopterans feed on liquids, although bees and a few wasps may also feed on pollen. Carrion represents an additional source of protein for some species and they will scavenge for dead animals in the wild. This paper aims at analyzing Hymenoptera visitors on a pig carcass during the process of decomposition, in the summer of 2005 and the winter of 2006 in Brazil, and comparing the results with other studies in the Neotropical region. To our knowledge, this is the first study which described the occurence of Agelaia pallipes, Polybia paulista and Scaptotrigona depilis on decomposing carcasses in southeastern Brazil. It also raises the hypothesis of possible applications of Hymenoptera to achieve more precise PMI estimations, apart from other insects already known as having great importance in such estimates.Considerável importância tem sido dada às construções de ninhos e transporte de alimento larval para o ninho como uma pré-condição para a eusociabilidade dos insetos. Muitos adultos de himenópteros alimentam-se em líquidos, embora as abelhas e poucas vespas podem também se alimentarem de pólen. Carcaças representam uma fonte adicional de proteína para algumas espécies e elas foram uma vez observadas se alimentando de animais mortos na natureza. Este trabalho tem por objetivo analisar Hymenoptera visitantes em carcaças de porcos durante o verão de 2005 e inverno de 2006 no Brasil, ao longo dos estágios de decomposição, comparando com resultados de outros estudos na região Neotropical. Pelo nosso conhecimento, esse é o primeiro estudo que descreveu a ocorrência de Agelaia pallipes, Polybia paulista e Scaptotrigona depilis em carcaças em decomposição no sudeste do Brasil. Isso também aumenta a hipótese na possibilidade de aplicação de Hymenoptera em auxiliar a estimar o IPM de maneira mais precisa, sem levar em conta insetos que já possuem grande importância forense.

  9. Efecto de la dieta de cerdos en crecimiento sobre el valor nutritivo y la aptitud tecnológica de la carne y grasa

    Directory of Open Access Journals (Sweden)

    Gustavo Capra

    2011-12-01

    Full Text Available Se sometieron cerdos con peso medio inicial de 53 kg a tres tratamientos: T1 dieta testigo conteniendo suplementos proteicos de origen vegetal y animal, T2 dieta basada exclusivamente en suplementos proteicos de origen vegetal y T3 dieta testigo restringida al 90 % de la cantidad ofrecida al T1 más libre acceso a pasturas. Se determinaron parámetros de comportamiento productivo y características de la canal en planta de faena. Se determinaron contenido de grasa intramuscular del Longissimus dorsi, perfil lipídico de la grasa subcutánea e intramuscular, contenido de hierro, zinc, magnesio, sodio y vitamina E. Se determinó también el punto de fusión de la grasa dorsal como indicador de aptitud tecnológica para la elaboración de productos fermentados. Con grasa procedente de cada tratamiento se elaboraron salames que fueron evaluados sensorialmente. En los parámetros de calidad de canal evaluados no hubo diferencias estadísticamente significativas. El acceso a pasturas determinó un incremento en el contenido de ácido linolénico y una mejora de la relación n6/n3 en la grasa subcutánea. No hubo diferencias significativas en el punto de fusión de la grasa. Las muestras de salame resultaron ser distintas, con un nivel de confianza del 95 % para los atributos textura e intención de compra.

  10. Desarrollo de una metodología in vitro para estimar la tasa de fermentación de los forrajes en el intestino grueso del cerdo

    Directory of Open Access Journals (Sweden)

    Leterme Pascal

    2005-12-01

    Full Text Available La metodología para estimar la tasa de fermentación de los forrajes se desarrolló en dos etapas. La primera, de digestibilidad enzimática, simula la digestión ocurrida en el estómago e intestino delgado, mediante el empleo de pepsina y pancreatina; en esta se determina la digestibilidad de la materia seca. La segunda etapa de fermentación simula la fermentación que ocurre en el intestino grueso utilizando como inóculo heces de cerdo; en esta se determina la cantidad de gas producido durante la fermentación de los forrajes y la degradación de la fibra detergente neutra (FDN. Las dos etapas presentaron buena repetibilidad en el tiempo. Abstract Develop of an in vitro methodology to determine the forages fermentation in to the large intestine. The methodology was developing in two phases. The first phase (enzymatic digestibility simulates the digestion occurred in the stomach and small intestine, by means of the employment of pepsin and pancreatin; and the digestibility of the dry matter is determined. The second phase (in vitro fermentation simulates the fermentation that occurs in the large intestine utilizing pig faeces; the quantity of gas produced during the fermentation of the feedstuff is determined and determine the degradation of neutral detergent fiber (NDF. All the phases show a good repeatability throw the time. Key words: digestibility in vitro, pigs, fermentación, forages (Azolla filiculoides, Xanthosoma saggitifolium, Morus alba, Trichanthera gigantea y Salvinia molesta.

  11. Jose carlos mariategui: sus articulos sobre arte

    OpenAIRE

    Henríquez, Cecilia

    2014-01-01

    En la prolífica obra que colma la corta vida del pensador latinoamericano,se encuentran profundas reflexiones sobre el arte que valela pena recordar en e1 año del centenario de su nacimiento. Los juiciosque propone sobre el arte están n1ediados por su pensamiento marxista.Aunque gran conocedor del arte de sus contemporáneos, sucompromiso socialista, cada vez mayor, reduce el espacio del análisisestilístico.Un punto de partida para observar el pensamiento estético deJosé Carlos Mariátegui es s...

  12. Estudio sobre el mercado laboral yerbatero, sus protagonistas, y sus luchas

    Directory of Open Access Journals (Sweden)

    María Angélica Jordán

    2013-12-01

    Full Text Available Reseña bibliográfica acerca del libro “Cosechando yerba mate. Estructuras sociales de un mercado agrario en el Nordeste argentino” (2012 de Victor Rau. Dicho estudio analiza el mercado laboral agrario yerbatero, recuperando su historicidad en la región, atendiendo a las estructuras sociales que han condicionando su funcionamiento, como las variables que permitieron la reconfiguración de la estructura del mercado actual, sobretodo a partir de los años ’90. Para ello, se enfoca en los asalariados agrícolas, sus condiciones de venta de fuerza de trabajo, y sus acciones colectiva de protesta.

  13. ESTUDIO DE LA DIVERSIDAD GENÉTICA DEL CERDO DOMÉSTICO EN EL DEPARTAMENTO DE CÓRDOBA (COLOMBIA UTILIZANDO MARCADORES MICROSATÉLITES

    Directory of Open Access Journals (Sweden)

    E. Pardo

    2015-01-01

    Full Text Available En este estudio se analizaron los niveles de diversidad y estructura genética de 161 cerdos domésticos pertenecientes a tres poblaciones del departamento de Córdoba, mediante 20 marcadores microsatélites. Todos los microsatélites utilizados resultaron polimórficos. Para todos los loci , el valor promedio de la heterocigosidad esperada fue mayor al valor promedio de la heterocigosidad observada, lo cual puede sugerir una posible endogamia en el sistema de apareamiento. e l índice F s T (0,12 ± 0,08 mostró un 88% de la varianza en las frecuencias alélicas reportadas dentro de cada población y solo el 12% de la varianza atribuible a diferencias entre poblaciones. Los valores de F is (0,079 y F i T (0,13, indican deficiencia de heterocigotos dentro de cada población y a nivel global. d esviaciones significativas del equilibrio Hardy-Weinberg ( p < 0,05 fueron observadas en ocho de los marcadores utilizados. e l árbol n eighbor-Joining obtenido reveló que Momil estuvo más estrechamente relacionada con Cereté, mientras Tierralta se mostró más alejada. e l análisis de componentes principales ( a CoP genera la individualización geográfica de cada población, siendo distante la población de Tierralta de las poblaciones Momil y Cereté, resultados similares a los obtenidos con la metodología n eighbor-Joining. e l programa s tructure con un K = 3, confirma la existencia de tres grupos o poblaciones distintas, generándose un patrón filogeográfico observado en la relación entre Momil, Cereté y Tierralta. e s importante señalar que son 3 grupos raciales diferentes, valiosos y deben conservarse.

  14. Eficacia de la moxibustión y el Arsenicum Álbum a la 30ch, como terapia alternativa en el tratamiento del síndrome diarreico en el cerdo

    Directory of Open Access Journals (Sweden)

    Ms.C. Florángel Vidal Fernández; Dr.C. Roberto Vázquez Montes de Oca; Dr. Rafael Leandro González; Msc. Erlán Pereira Pargas; Dr. Iván Peña García; Dr. Arnaldo del Toro Ramírez.

    2005-02-01

    Full Text Available Se realizó un ensayo clínico fase II, abierto, no secuencial y aleatorizado en 45 cerdos de preceba que padecían del síndrome diarreico en el complejo agroindustrial “Camujiro” en la provincia de Camagüey. La muestra se dividió en tres grupos, dos de estudio (A y B y un grupo control (C; al grupo control se le administró enrocilina con el objetivo de valorar la acción terapéutica de esta, respecto a las técnicas de medicina natural que fueron sometidos los dos restantes grupos, con vista a reducir el uso del antibiótico antes mencionado. Los resultados estadísticos prueban la efectividad de estos esquemas de tratamientos, existiendo diferencia significativas en cuanto a la proporción de cerdos recuperados (P< 0,05. Quedo demostrado que las técnicas de medicina natural ensayadas, la moxibustion (Grupo A y el Arsenicum Álbum (Grupo B resultó más efectivo y más económico que la terapia convencional permitiéndonos reducir el uso de la Enrocilina utilizada para combatir el síndrome diarreico en el cerdo. He was carried out a rehearsal clinical phase II, open, not sequential and randomised in 45 Prefeeds pigs that suffered of the syndrome diarrheic in the agroindustrial complex "Camujiro" in the county of Camagüey. The sample was divided in three groups, two of study (A and B and a group control (C; to the group control he/she was administered Enrocilina with the objective of valuing the therapeutic action of this, regarding the techniques of natural medicine that were subjected the two remaining groups, with view to reduce the use of the antibiotic before mentioned. The statistical results prove the effectiveness of these outlines of treatments, existing significant difference as for the proportion of recovered pigs (P <0,05. I am demonstrated that the rehearsed techniques of natural medicine, the moxibustion (Group TO and the Arsenicum Album (Group B it was more effective and more economic than the conventional therapy

  15. La estructura del fullereno C60 y sus aplicaciones

    OpenAIRE

    VASILIEVNA KHARISSOVA, OXANA; Ortiz Méndez, Ubaldo

    2002-01-01

    Se revisan los datos reportados de una nueva clase de materiales basados en los fullerenos. Se explica la estructura del fullereno C60 y sus compuestos con metales alcalinos, sus propiedades y aplicaciones. Se señala que estos compuestos tienen propiedades de superconducción comparable con la de los tradicionales cupratos. Se indica que esta modificación del carbono puede formar nanotubos que tienen varias aplicaciones prácticas debido a sus propiedades.

  16. Viscocidad de la Dieta y su Efecto sobre la Pérdidad de Aminoácidos Endógenos Recuperados en Íleon Terminal de Cerdos

    OpenAIRE

    Edgar Chi-Moreno; Miguel Cervantes-Ramírez; J. Luis Figueroa-Velasco; Jorge Baeza-López; Manuel Cuca-García; Fernando Copado-Bueno; Jorge L. Yáñez-Hernández; Noemí Torrentera-Olivera

    2005-01-01

    La viscosidad de la dieta provocada por algunos compuestos antinutricionales en los cereales puede afectar la pérdida de aminoácidos (AA) endógenos. Por tanto, se realizó un estudio para evaluar el efecto de la viscosidad de la dieta, producida por la adición de goma Guar, en la pérdida de AA endógenos recuperados al final del intestino delgado. Seis cerdos (62.0±2.5 kg peso) con cánulas en íleon terminal se utilizaron en tres periodos experimentales en un diseño en Cuadro Latino 3×3 repetido...

  17. La calidad del suelo y sus indicadores

    Directory of Open Access Journals (Sweden)

    Angélica Bautista Cruz

    2004-01-01

    Full Text Available A pesar de la importancia para la vida, el suelo no ha recibido de la sociedad la atención que merece. Su degradación es una seria amenaza para el futuro de la humanidad. Por lo tanto, los científicos se enfrentan al triple desafío de intensificar, preservar e incrementar la calidad de la tierra. Para ello, es necesario contar con una sólida concepción de la calidad y con indicadores de calidad o salud de la tierra y de manejo sostenible de la misma, tal como se cuenta para dar seguimiento a variables sociales y económicas. En este trabajo se realiza una revisión de los principales conceptos relacionados con la calidad del suelo y sus indicadores. El adecuado manejo de los conceptos sobre estos temas debe redundar en un mejor manejo de la sostenibilidad del recurso, de la agricultura sostenible y en la toma de decisiones de políticas de uso del suelo. El desarrollo de indicadores de calidad del suelo debería basarse en el uso de este recurso y en la relación entre los indicadores y la función del suelo que se esté evaluando. Deben considerarse propiedades edáficas que cambien en un periodo de tiempo relativamente corto.

  18. El bienestar animal en la reproducción y producción de cerdos - The animal welfare in the reproduction and production of pigs

    Directory of Open Access Journals (Sweden)

    Alejandro Córdova Izquierdo

    2007-12-01

    Full Text Available RESUMENEl bienestar animal es una condición ideal, resultado de la aplicación de normas específicas, adecuadas y posibles, sobre los sistemas y procesos involucrados a lo largo de toda la cadenaproductiva, que permiten a los animales vivir en las mejores condiciones posibles, sin padecer sufrimientos físicos o psicológicos innecesarios. Para todos los animales y en especial para aquellos cuyo destino será servir de fuente de alimentos al hombre, se antensifica el compromiso ético de brindarles a lo largo de su vida productiva las mejores condiciones posibles de hábitat, sanidad, manejo, alimentación y cuidados en general. En la actualidad,conceptos de bienestar animal, son cuestión de interés público complejo y multifacético que incluye importantes dimensiones científicas, éticas, económicas y políticas. Por ser un temade importancia creciente en la sociedad, el bienestar animal ha de abordarse sobre bases científicas verdaderas. Las causas de los problemas de bienestar animal, se deben a la percepción errónea acerca de los animales, como seres que no sienten y que por lo tanto, no son capaces de sufrir. Es fácil que se desarrollen actitudes negativas hacia los animales, lo cual se refleja en conductas de negligencia, crueldad o trato irrespetuoso. Los productores,médicos veterinarios, así como la sociedad en general, concientes del cuidado de los animales, saben la importancia de conocer los aspectos del confort de los animales ya que la fisiología, el desarrollo y el comportamiento del animal, son afectados por las malas condiciones ambientales, de producción y de manejo en general. En esta revisión, se presentan aspectos relacionados con el bienestar animal en la reproducción y producción de cerdos.ABSTRACTThe animal wefare is an ideal condition, result of the application of specific, appropriate and possible norms, on the systems and processes involved along the whole productive chain thatyou/they allow to the

  19. Los directores aprendiendo de sus maestros

    Directory of Open Access Journals (Sweden)

    Manuel Ortega Muñoz

    2016-07-01

    Full Text Available El presente artículo de investigación está enmarcado dentro de la línea de indagación Aprendizaje para la Gestión y versa sobre los principales aprendizajes que han adquirido los directivos de educación básica del estado de Durango, México, para el mejor ejercicio de su función. Desde un estudio de narrativa, se recopilaron historias de 16 directores de educación primaria estatal pertenecientes a la zona escolar 24 del sector educativo no. 1 de la Secretaría de Educación Pública. El análisis de los datos se llevó a cabo mediante la herramienta de análisis de información cualitativa Atlas ti versión 7.5. Los hallazgos de la investigación muestran que los principales aprendizajes que los directivos de educación básica del estado de Durango, México han adquirido de sus maestros para el mejor ejercicio de su función son dos: a el trabajo en equipo, del cual se desprenden componentes como una eficaz y eficiente organización del trabajo, el trabajar siempre en colaboración y tener la mejor de las actitudes para trabajar; y b el liderazgo compartido, aspecto en que se perciben elementos como el óptimo manejo y resolución de conflictos, el liderazgo y la toma de decisiones en conjunto por parte del colectivo escolar. Abstract This research paper, framed within the line of inquiry Learning Management, deals with the main lessons that have acquired the management of basic education in the state of Durango, Mexico, for the best performance of their duties, from a study of narrative histories of 16 directors of state primary education outside the school zone 24 the education sector 1 of the Ministry of Education. The data analysis was performed using the computer statistical package you Atlas ti version 7.5. The research findings show that there are two main lessons that have acquired the management of basic education in the state of Durango, Mexico from their teachers for the better performance of their function: a teamwork

  20. Laser bendability of SUS430/C11000/SUS430 laminated composite and its constituent layers

    Science.gov (United States)

    Hossein Seyedkashi, S. M.; Gollo, Mohammad Hoseinpour; Biao, Jin; Moon, Young Hoon

    2016-05-01

    Laminated composites are of great interest in different industries while having the advantages of all base metals. In this research, the laser bending of a three-layered SUS430/C11000/SUS430 laminated composite is characterized both experimentally and numerically. This composite can be used in the microelectronics industry since it has the anti-corrosion and strength capability of stainless steel, and the electrical superiority of copper. The specimens are bent using a Ytterbium fiber laser irradiated on a straight path along the sheet width. The effects of bending parameters including the number of passes, scanning velocity, beam diameter, laser power and delay time between passes are examined for a three-layered laminated sheet, and compared with its constituent steel and copper layers. It is found that the thin copper mid-layer strongly affects the rate of bending per pass. Heat distribution and plastic strain along the thickness during the process are characterized by using the finite element method. The Cu mid-layer decreases the bending angle, but also postpones the onset of melting, and thus can be compensated by the application of higher laser powers. It is shown that the bending angle increases with an increase in laser power and delay time, and a decrease in laser velocity and beam diameter.

  1. Ocho diferentes especies de parásitos gastrointestinales fueron identificadas en cerdos de traspatio en El Municipio de El Sauce - León. Nicaragua (Eight different species gastrointestinal parasites were identified in free roaming pigs in EL Sauce - Leon. Nicaragua

    Directory of Open Access Journals (Sweden)

    Luz A. Luna

    2005-10-01

    Full Text Available Este trabajo se realizó con cerdos criados en traspatio en el municipio de El Sauce, Departamento de León, Nicaragua En el primer estudio se determinó la prevalencia de Parásitos Gastrointestinales (PGI en 60 cerdos de patio sacrificados en matadero. Se identificaron 6 tipos de especies de parásitos gastrointestinales: Macrachantorinchus hirudinaceu, Oesophagustomun spp, Áscaris suum, Trichuris suis e Hyostrongylus rubidus, siendo este ultimo el de mayor prevalencia. Se determinó la prevalencia de PGI en heces de cerdos en dos grupos de edades. Se identificaron los helmintos Ascaris suum, Hyostrongylus, rubidus, Strongiloides ransomi, Oesophagostomun spp, y Trichuris suis. Los protozoos encontrados fueron Isospora suis y Eimeria sp. Con una mayor frecuencia se encontró Ascaris suum (42.86% e Hyostrongylus (39.80%, en el grupo mayor de seis meses, en el grupos menor de seis meses los más frecuentes eran Áscaris suum (48.98% y Trichuris (45.92%. La intensidad de infestación de H. rubidus fue significativamente más alto en grupo de cerdos mayores de seis y T. suis e Isospora suis tuvieron diferencia significativa en el grupo menor de seis meses. En resumen identificamos ocho especies de parásitos gastrointestinales en los cerdos de traspatio, que son de importancia económica. This work was done with free roaming pigs in El Sauce, Department of Leon, Nicaragua. In the first studies the prevalence of gastrointestinal parasites were found in 60 pigs in the slaughterhouse where there was 6 types of gastrointestinal parasites identified: Macrachantorinchus hirudinaceus, Oesophagostomun spp, Ascaris suum, Trichuris suis, Hyostrongylus rubidus, the last one with the biggest prevalence. Later the prevalence of gastrointestinal parasites in feces was found in two groups with different age, where the helminths Ascaris suum, Hyostrongylus rubidus, Strongyloides ransomi, Oesophagostomun spp, and Trichuris suis were identified. The protozoo that

  2. Proteja a sus seres queridos de un ahogamiento

    Centers for Disease Control (CDC) Podcasts

    2008-12-10

    Este podcast, que forma parte de la iniciativa Proteja a sus seres queridos, aborda las medidas que pueden tomar los padres de familia para ayudar a proteger a sus niños de un ahogamiento, una de las principales causas de lesiones infantiles.  Created: 12/10/2008 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 4/1/2009.

  3. Proteja a sus seres queridos de las intoxicaciones

    Centers for Disease Control (CDC) Podcasts

    2008-12-10

    Este podcast, que forma parte de la iniciativa Proteja a sus seres queridos, aborda las medidas que pueden tomar los padres de familia para ayudar a proteger a sus niños de una intoxicación, una de las principales causas de lesiones infantiles.  Created: 12/10/2008 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 4/1/2009.

  4. Proteja a sus seres queridos de las quemaduras

    Centers for Disease Control (CDC) Podcasts

    2008-12-10

    Este podcast, que forma parte de la iniciativa Proteja a sus seres queridos, aborda las medidas que pueden tomar los padres para ayudar a proteger a sus niños de las quemaduras, una de las principales causas de lesiones infantiles.  Created: 12/10/2008 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 4/1/2009.

  5. Proteja a sus seres queridos de las lesiones viales

    Centers for Disease Control (CDC) Podcasts

    2008-12-10

    Este podcast, que forma parte de la iniciativa Proteja a sus seres queridos, aborda las medidas que pueden tomar los padres de familia para ayudar a proteger a sus niños de las lesiones viales, una de las principales causas de lesiones infantiles.  Created: 12/10/2008 by National Center for Injury Prevention and Control (NCIPC).   Date Released: 4/1/2009.

  6. DESCRIPCIÓN DE COMPONENTES QUÍMICOS Y COEFICIENTES ALOMÉTRICOS EN CERDOS CASTRADOS HASTA LOS 273 DÍAS EN EL TRÓPICO ALTO COLOMBIANO

    Directory of Open Access Journals (Sweden)

    GA Casas

    2007-01-01

    Full Text Available Las relaciones entre la ingestión de nutrientes y la composición química y física corporal son afectadas a nivel práctico por un rango de factores asociados con la nutrición, el genotipo, el ambiente y el estado de madurez, y constituyen la base para manipular la calidad de la carne y la eÀciencia en un contexto especíÀco de producción. El objetivo de esta investigación fue describir los componentes químicos de un genotipo de cerdos, y las relaciones de tipo alométrico que se generan entre los diferentes componentes, teniendo como referencia la proteína en la canal y en el peso corporal vacío, en un contexto especíÀco y comercial de producción. Se utilizaron 39 cerdos castrados del cruce Duroc-Landrace x Yorkshire, desde el nacimiento hasta los 273 días de edad, los cuales fueron sometidos a prácticas convencio-nales comerciales de manejo. Después de los 70 días los animales fueron trasladados a 2600 msnm (14 ºC. Cuatro cerdos fueron sacriÀcados a 1, 21, 63, 99, 126, 154, 175, 210, 239 y 273 días de. Se estimaron los contenidos de agua, proteína, lípidos y cenizas en canal, vísceras rojas y vísceras blancas, mediante análisis proximal. Los animales fueron alimentados con dietas comerciales cuya composición fue determinada. Se evaluaron seis ecuaciones para estimar la predicción de las relaciones alométricas entre los componentes químicos y la pro-teína. Se conÀrmó que la proteína, los lípidos y el agua se incrementaron de forma lineal y cuadrática mientras que la ceniza lo hizo de forma lineal, tanto en la canal como en el cuerpo vacío. La proporción de los componentes químicos en la canal y cuerpo vacío mostraron diferentes comportamientos a lo largo del ciclo de crecimiento. Se obtuvieron relaciones lípido:proteína de 2,14, agua:proteína de 3,22, y cenizas:proteína de 0,16. Las ecuaciones alométricas aumentadas y linealizadas fueron las que mejor describieron los datos, mostran-do coe

  7. Estudio digital radiográfico y densitométrico en mandíbulas de cerdos sometidos a osteotomía con alta y baja velocidad, con refrigeración líquida Digital and densitometric radiographic study in swine jaws subjected to osteotomy using high and low drilling speeds with liquid cooling

    Directory of Open Access Journals (Sweden)

    Lira Marcela Monti

    2010-09-01

    Full Text Available Se evaluó por medio de la radiografía digital y la densitometría, la influencia de un dispositivo de rotación para el corte en el proceso de curación del hueso después de osteotomías en mandíbulas de cerdos, a tiempos controlados. Se realizaron dos secciones transversales en la base de la mandíbula derecha, una con una alta velocidad de rotación y otra por la acción de baja velocidad, ambos con el líquido de irrigación. Tras los tiempos preestablecidos, los animales fueron sacrificados y sus mandíbulas retiradas para los estudios radiográficos y de densitometría radiológica con los sistemas digitales Cromox, Digora y ODR. En los períodos iniciales (7 y 28 días el equivalente a la densitometría en milímetro de aluminio y la media de niveles de gris fueron más altos en la región de osteotomías hechas con alta rotación, y en los períodos finales (60 y 90 días las mayores densitometrías fueron para osteotomías hechas con baja velocidad, lo que indica una mejor cicatrización final del hueso cuando se hace uso de la baja velocidad. El análisis cualitativo del proceso de reparación fue realizado por el ODR, lo que permitió la recopilación de imágenes digitales en color y en tres dimensiones, para la comparación del espesor del hueso con una cuña de aluminio, indicando que en los 28 días de curación del hueso, ya estaba evidente la completa reparación.The influence of the type of rotary cutting instrument on bone repair after osteotomy in swine jaw, was evaluated using digital and densitometric radiographic examinations, at controlled times. Two cross-sectional dissections were made through the base of the right jaw, one using a high speed rotary instrument and the other using low speed, both with liquid cooling. After established periods the animals were sacrificed and their jaws removed for direct and indirect digital radiographic and densitometric studies using the CROMOX, DIGORA and ODR systems. In the initial

  8. La violencia de hijos adolescentes contra sus progenitores La violencia de hijos adolescentes contra sus progenitores

    Directory of Open Access Journals (Sweden)

    Concepción Aroca Montolío

    2013-10-01

    Full Text Available According to Prosecutor’s Office of the Minor, the accusation interposed by mothers and/or fathers victims by theirs children, along 2007 were 2603, in 2008 amounted 4.211, in 2009 there were 5.209 and in 2010 there were 8.000 accusations. Suede this worrying increase, the principal aim of our article is to check the scientific international and national documentation, from 1957 until the year 2010 that analyses the phenomenon of the adolescent violence against parents, to achieve an approximation to its keys that there allows us the comprehension and analysis of this serious familiar problem. For it we will analyse: (a the importance of this crime by means of criminological mediators: prevalence and incidence; (b the age and sex variables’ aggressors to be able to establish a basic profile about theirs and, (c the violence types that the teenagers wield to damage, prejudice and suffering against their progenitors, with the aim to obtain what they want. The information obtained in this research review and qualitative analysis, change in base to the methodology used and the type of sample under study to obtain conclusions. Even though, we wantto do research into needs to investigate this type of familiar violence, and from there, to do researches with rigorous scientific methodologies, unifying criteria and variables to be investigating, to be able to anticipate in this increasing problem that the parents have. Según la Fiscalía del Menor en el año 2007, las denuncias interpuestas por madres y/o padres, víctimas de malos tratos por sus hijos e hijas menores de edad, fueron 2.683. En 2008 ascendieron a 4.211, en 2009 se presentaron 5.209 y en el año 2010 se registraron 8.000 denuncias. Ante éste preocupante incremento, el objetivo principal de nuestro artículo es revisar la documentación científica que analiza la violencia filio-parental,  desde 1957 hasta el año 2011, para lograr una aproximación a sus claves que nos permita la

  9. Evaluación de técnicas de procesamiento y almacenamiento de piel cadavérica para bancos de tejidos

    OpenAIRE

    2015-01-01

    En el presente trabajo se reproducen y analizan algunas de las técnicas más comunes a nivel internacional para el manejo de piel cadavérica para bancos de tejidos. Para ello, se llevó a cabo la ablación, el procesamiento, la desinfección y el almacenamiento de piel utilizando un modelo animal (cerdo, Sus scrofa domesticus). Para verificar la calidad microbiológica del tejido se compararon dos diferentes métodos de desinfección (antibióticos y desinfección química con cloro). Además, se evaluó...

  10. Inmigrantes andinos en Madrid: Sus danzas y sus músicas tradicionales

    Directory of Open Access Journals (Sweden)

    Lourdes Morales

    2007-01-01

    Full Text Available In our urban context we get in touch with people that have been forced to leave their countries under different circumstances in order to live in Spain. This article analyses their wish to maintain a group identity and how this identity is materialized by some individuals through traditional dance and music. The study is intended as a framework for subsequent educational investigations that relate that cultural reality with the situation in secondary schools where children of Latin American immigrants study.Nuestro entorno ciudadano inmediato nos pone en contacto diario con personas que por diferentes razones se han visto obligadas a dejar sus países de origen para instalarse en España. Este artículo analiza el deseo que surge en ellas de mantener una identidad de grupo, y cómo ésta se concreta en ciertos individuos mediante manifestaciones de danza y música popular tradicional. El estudio pretende servir de marco para ulteriores investigaciones educativas que vinculen esa realidad cultural con la situación de institutos de enseñanza secundaria en los que estudian hijos de inmigrantes latinoamericanos.

  11. Internações no sistema de serviços hospitalares, SUS e não SUS: Brasil, 2006 Hospital admissions in the hospital health care system, SUS and non-SUS: Brazil, 2006

    Directory of Open Access Journals (Sweden)

    Marizélia Leão Moreira

    2011-09-01

    Full Text Available O objetivo deste estudo foi descrever as admissões, SUS e não SUS, no Sistema de Serviços Hospitalares no Brasil no ano de 2006, adotando o indivíduo internado como unidade de análise. As fontes dos dados foram o Sistema de Informação Hospitalar (SIH/SUS e de Comunicação de Internação Hospitalar (CIH referentes a 2006 e 2007. A identificação do indivíduo foi feita pelo método probabilístico de associações de registros (linkage e para a composição dos dados da internação aplicaram-se algoritmos de composição nos registros de cobranças e de comunicações das internações. Foram analisadas 12.391.990 internações com ao menos um dia de duração no ano de 2006. A natureza do método probabilístico que encerra certo grau de imprecisão, a adoção de parâmetros conservadores a fim de evitar a inclusão de falsos positivos, tanto quanto a subnotificação da CIH representam as possíveis limitações do estudo. O Sistema de Serviços Hospitalares no Brasil apresentou taxa de hospitalização de 5,6%, sendo 5,0% SUS e 1,6% não SUS, diferenciando-se segundo Unidade da Federação de ocorrência. Os dados das internações não financiadas pelo SUS coletados pela CIH agregam informações importantes para a análise da assistência hospitalar no país. Os dados administrativos do SUS são válidos para análises de internações e os algoritmos de composição dos dados de internação, a partir da cobrança, aprimoram a análise do Sistema de Serviços Hospitalares no Brasil.The objective of this study was to analyze admissions in the hospital health care system in Brazil in the year of 2006, starting from the admitted subjects. The initial data were the admissions SUS (SIH, non-SUS (CIH in 2006 and 2007. The identification of the individual was by probabilistic method of associations of registrations (linkage and, for the composition of the admission data, specific algorithms were applied to the data. A total of 12

  12. Parámetros diferenciadores del tipo de alimentación en el cerdo ibérico y aplicación de tecnologías inteligentes para su clasificación

    Directory of Open Access Journals (Sweden)

    Guerrero Molina, M. A.

    1996-06-01

    Full Text Available In this work a significant Extremadura South-east Iberian pigs fatty acids, and his capacity of differentiate qualities was studied. Furthermore, and based on this capacity the definition of confidence intervals and also differentiate parameters was obtained.
    They were used to develop a computer program system which allow the automatically classification of pigs. This system uses one of the most powerful sample classification tools existing nowadays: Artificial Neuronal Networks (A.N.N..

    En el presente trabajo se realiza un estudio sobre los ácidos grasos y su capacidad de diferenciar calidades en una población significativa de cerdos ibéricos en el sudeste de Extremadura (Monesterio-Badajoz. Basándonos en esta capacidad diferenciadora se han obtenido los intervalos de confianza y por lo tanto, unos parámetros diferenciadores.
    Con ellos desarrolla un sistema informático que permite la clasificación automática de estas canales. Este programa utiliza una de las herramientas más potentes que existen hoy en día para la clasificación de cualquier tipo de muestras: Las Redes de Neuronas Artificiales (RNA.

  13. Efecto del tiempo de almacenamiento, el tipo de músculo y el genotipo del animal sobre las pérdidas por goteo en carne cruda de cerdo

    Directory of Open Access Journals (Sweden)

    Bermúdez M Fanhor

    2009-09-01

    Full Text Available En un diseño factorial con arreglo en parcelas subdivididas se analizó el efecto del tiempo de almacenamiento (24, 48 y 72 h a 6 °C, el tipo de músculo (Longissimus dorsi, Tríceps brachii, Bíceps femoris y el genotipo de animal (tres razas puras: Yorkshire, Landrace, Duroc; dos genotipos: F1 por el cruce Yorkshire x Landrace (YL, cruce de la F1 x Duroc (F1D sobre las pérdidas por goteo en carne cruda de cerdo. Las muestras se tomaron a las 24 h postmortem y se almacenaron durante 72 h. El mayor porcentaje de pérdida se presentó para la carne obtenida a partir del músculo Bíceps femoris proveniente de los animales pertenecientes al genotipo F1. El porcentaje de pérdida por goteo más alto se presentó durante las primeras 24 h. Los resultados indicaron diferencias estadísticas altamente significativas (P < 0.01 para los efectos individuales genotipo, tipo de músculo y tiempo de almacenamiento sobre el porcentaje de pérdida por goteo.

  14. Lorna Goodison y sus representaciones caribeñas

    Directory of Open Access Journals (Sweden)

    Inmaculada de Jesús Arboleda Guirao

    2016-12-01

    Full Text Available A través de sus poemas y relatos breves, Lorna Goodison refleja la evolución de la mujer caribeña: la aceptación de su pasado y su apariencia, en definitiva, la aceptación de su identidad. La esclavitud, belleza y maternidad son temas recurrentes en sus trabajos. Goodison representa una esclavitud física pero también mental (esclavitud a la belleza de la sociedad americana como contraposición a la caribeña y final libertad y aceptación de su identidad como mujer y madre.

  15. Lorna Goodison y sus representaciones caribeñas

    OpenAIRE

    Inmaculada de Jesús Arboleda Guirao; M. Esther Mediero Durán

    2016-01-01

    A través de sus poemas y relatos breves, Lorna Goodison refleja la evolución de la mujer caribeña: la aceptación de su pasado y su apariencia, en definitiva, la aceptación de su identidad. La esclavitud, belleza y maternidad son temas recurrentes en sus trabajos. Goodison representa una esclavitud física pero también mental (esclavitud a la belleza de la sociedad americana como contraposición a la caribeña) y final libertad y aceptación de su identidad como mujer y madre.

  16. Origem e distribuição do nervo axilar em fetos de suínos (Sus scrofa domesticus - Linnaeus, 1758) do cruzamento das linhagens Dan Bred e AGPIC337

    OpenAIRE

    França, Gustavo Lucio Monteiro de

    2015-01-01

    Investigou-se a origem e distribuição do nervo axilar em 30 fetos de suínos do cruzamento das linhagens Dan Bred e AGPIC337, sendo 15 fêmeas e 15 machos, após a fixação do material em solução aquosa de formaldeído a 10%. O nervo axilar originou dos ramos ventrais de c6 e c7 em 22 exemplares (73,33%), sendo 10 fêmeas (33,3%) e 12 machos (40,00%) e de c5, c6 e c7 nos oito exemplares restantes (26,66%), sendo cinco fêmeas (16,66%) e três machos (10,00%). Quanto à distribuição, o nervo axilar emi...

  17. Survey and Control of Wild Boar Sus scrofa Damage in Qingyun Forest, Heilongjiang Province%黑龙江青云林场野猪危害调查及防治

    Institute of Scientific and Technical Information of China (English)

    张丹; 刘丙万

    2012-01-01

    人和野猪的冲突已经成为日益关注的问题。2010年6-10月,在黑龙江省尚志市青云林场利用走访和问卷调查对野猪危害进行了研究,并通过散布东北虎粪便、放置东北虎图像、播放东北虎声音的方法对野猪危害进行了防治并与当地居民防治野猪危害的措施进行了比较。结果如下:(1)野猪危害多在夏初至秋末,主要危害玉米和西葫芦,受危害的农田多是林缘地和林间地。(2)把野猪第2次进入农田与第1次进入农田的间隔天数作为防治措施的有效性评估指标,研究发现利用东北虎粪便、图像、声音防治野猪危害的有效期分别是3.00±0.58d、1.33±0.33d、5.25±0.63d。(3)林场84.09%的居民会采取防治措施,防治措施有效期一般是3-7d。(4)利用东北虎粪便和图像防治野猪危害的效果与林场大多数居民采取的防治措施效果相比差异不显著(P〉0.05)。利用声音防治危害的效果只与利用塑料绳围栏(P=0.029)和干扰物(P=0.029)的防治效果相比差异显著。利用东北虎粪便、图像和声音防治野猪危害效果不理想可能和这个地区东北虎消失时间比较长有关。%In recent years, the frequency of conflicts between wild boar and humans has increased. From June to October 2010, we surveyed crop damage caused by wild boar in Qingyun forest in Shangzhi County, Heilongjiang Province using questionnaires and field investigations. Wild boar damage took place almost from early summer to late autumn and the main crops damaged by wild boar were corn and squash, mainly in fields located near the forest edge or in the forest. We conducted field experiments to evaluate the effectiveness of various measures intended to deter wild boar from damaging crops. Measures includ-ed placement of tiger faeces at the perimeter of crop areas, playing of recorded tiger vocalizations, placement of life - sized photos of tiger faces at crop perimeters, and placement of life-sized models of Siberian tigers at crop perimeters. We defined an index for the effectiveness of these measures as the interval between the first entry of boar to a given field ( the date when the preventive measure was installed) and the next entry of boar to the same field. The mean number of effective days when using faeces, image models and vocalizations of Siberian tiger were 3.00±0. 58 days, 1.33±0. 33 days and 5.25 ±0. 63 days, re-spectively. Most of the local people in Qingyun ( 84. 09% ) implemented other measures whose effectiveness lasted from 3 to 7 days. The mean number of effective days was not significantly different between faeces and image models of the tiger and the traditional prevention measures implemented by the inost of the local residents ( P 〉 0. 05 ) . While the mean effective days showed significant difference between sounds of tiger and plastic fence ( P = 0. 029 ) and disruptors ( P = 0. 029 ) implemented by the forest residents. This might be explained in part by the fact that the native Siberian tiger disappeared many years earlier from Qingyun forest farm, Heilongjiang.

  18. Hypoxanthine-guanine phosphoribosyltransferase and inosine 5′-monophosphate dehydrogenase activities in three mammalian species: aquatic (Mirounga angustirostris), semi-aquatic (Lontra longicaudis annectens) and terrestrial (Sus scrofa)

    OpenAIRE

    Barjau Pérez-Milicua, Myrna; Zenteno-Savín, Tania; Crocker, Daniel E.; Gallo-Reynoso, Juan P.

    2015-01-01

    Aquatic and semiaquatic mammals have the capacity of breath hold (apnea) diving. Northern elephant seals (Mirounga angustirostris) have the ability to perform deep and long duration dives; during a routine dive, adults can hold their breath for 25 min. Neotropical river otters (Lontra longicaudis annectens) can hold their breath for about 30 s. Such periods of apnea may result in reduced oxygen concentration (hypoxia) and reduced blood supply (ischemia) to tissues. Production of adenosine 5′-...

  19. Analysis of Codon Bias of MC1R Gene in Pig (Sus scrofa)%猪黑皮质素受体1基因密码子偏好分析

    Institute of Scientific and Technical Information of China (English)

    肖红卫; 刘西梅; 郑新民; 华文君; 李莉; 张立苹; 毕延震; 华再东

    2013-01-01

    遗传密码子是生命信息的基本遗传单位,每种氨基酸对应1~6个同义密码子。特定物种在长期进化中形成了适应自身基因环境的密码子使用偏好。运用CHIPS、CUPS和CodonW程序分析猪黑皮质素受体1基因密码子偏好,并与牛、羊、小鼠、人等多种动物的黑皮质素受体1基因密码子偏好进行比较,以期为转基因动物育种提供依据。结果表明,猪偏好使用以C、G结尾的密码子(96.88%),且在整个编码区序列中G+C含量(67.81%)大于A+T(32.19%),该基因在猪体内表达水平很高(CAI=0.849),并且发现,猪的密码子偏好性与牛、犬等动物类似,明显不同于鲀、雀、獾、大猩猩等动物。要实现目的基因在猪MC1R基因中进行定点整合并成功表达和尽可能地提高其表达量,需对目的基因的部分密码子进行改造。%Genetic codon is the basic hereditary unit of life information , and each amino acid is corresponding to 1~6 synony-mous codons.Specific organisms formed codon bias in long -term evolution to adapt their genetic environment .In this paper, the codon bias of MC1R gene in the pig was analyzed by Codon W , CHIPS and CUSP programs , and it was compared with that in vari-ous animals, such as cattle, sheep, mice, human etc., so as to provide a basis for transgenic animals breeding .The results showed that the pig preferred to use the codons ending with C and G (96.88%), the content of G+C (67.81%) was higher than that of A+T (32.19%) in the whole coding sequence , and MC1R gene in the pig had very high expression level (CAI=0.849).It was also found that the codon usage bias of the pig was similar to that of cattle , dog and so on , while it was obviously different from that of Takifugu rubripes, Taeniopygia guttata, Sarcophilus harrisii, Gorilla gorilla etc..In order to realize the site-specific integration of target gene into pig MC1R gene as well as its successful and high -efficient expression , the alteration of some codons should be conducted in target gene .

  20. The effects of colloids or crystalloids on acute respiratory distress syndrome in swine (Sus scrofa models with severe sepsis: analysis on extravascular lung water, IL-8, and VCAM-1

    Directory of Open Access Journals (Sweden)

    Rismala Dewi

    2016-04-01

    Full Text Available Background: Acute respiratory distress syndrome (ARDS is a fatal complication of severe sepsis. Due to its higher molecular weight, the use of colloids in fluid resuscitation may be associated with fewer cases of ARDS compared to crystalloids. Extravascular lung water (EVLW elevation and levels of interleukin-8 (IL-8 and vascular cell adhesion molecule-1 (VCAM-1 have been studied as indicators playing a role in the pathogenesis of ARDS. The aim of the study was to determine the effects of colloid or crystalloid on the incidence of ARDS, elevation of EVLW, and levels of IL-8 and VCAM-1, in swine models with severe sepsis.Methods: This was a randomized trial conducted at the Laboratory of Experimental Surgery, School of Veterinary Medicine, IPB, using 22 healthy swine models with a body weight of 8 to 12 kg. Subjects were randomly allocated to receive either colloid or crystalloid fluid resuscitation. After administration of endotoxin, clinical signs of ARDS, EVLW, IL-8, and VCAM-1 were monitored during sepsis, severe sepsis, and one- and three hours after fluid resuscitation. Analysis of data using the Wilcoxon test , Kolmogorov-Smirnov test, Mann-Whitney test, unpaired t test.Results: Mild ARDS was more prevalent in the colloid group, while moderate ARDS was more frequent in the crystalloid group. EVLW elevation was lower in the colloid compared to the crystalloid group. There was no significant difference in IL-8 and VCAM-1 levels between the two groups.Conclusion: The use of colloids in fluid resuscitation does not decrease the probability of ARDS events compared to crystalloids. Compared to crystalloids, colloids are associated with a lower increase in EVLWI, but not with IL-8 or VCAM-1 levels.

  1. Identification and Analysis of Alternative Splicing Events in Sus Scrofa Using RNA-Seq Data%基于RNA-seq测序数据鉴定和分析猪基因组可变剪接事件

    Institute of Scientific and Technical Information of China (English)

    冉茂良; 陈斌; 李智; 董莲花; 贺长青; 柳小春

    2016-01-01

    可交剪接作为一种增加基因组和蛋白质组多样性的重要机制,广泛发生于真核生物基因转录的过程中.本文采用Illumina Hiseq 2500测序平台对猪睾丸组织4个不同发育时期(60胚龄、90胚龄、30日龄和180日龄)进行转录组测序,以猪基因组数据为参考,鉴定和分析了猪基因组可变剪接事件.从猪基因组中鉴定出20398个基因(80.6%)对应的92738个可变剪接事件.在不同的可变剪接类型中,以第一个外显子可变剪切(alternative 5' first exon,TSS)、最后一个外显子可变剪切(alternative 3'last exon,TTS)、单外显子跳跃(skipped exon,SKIP)和可变5'或3'端剪切(alternative exon ends,AE)4种类型为主,分别占所有可变剪接事件的41.0%,32.9%,7.6%和7.4%.GO功能富集分析显示,发生可变剪接的基因主要富集于物质合成、物质结合及酶活性相关的GO项中,而各发育时期特异的可变剪接基因与发育时期的生理状态密切相关,60胚龄时主要与酶活性和组织形成相关,30日龄时主要与抗环境应激和离子通道活性相关,180日龄时则主要与循环系统相关.此外,在筛选出64个与睾丸素代谢相关的基因中,63个基因发生可变剪接,且以TSS和TTS为主,表明这两种可变剪接类型与睾丸素合成和分泌密切相关.通过对猪基因组可变剪接的分析,为深入研究可变剪接生物学功能及进一步开展分子育种工作提供理论依据.

  2. NCBI nr-aa BLAST: CBRC-PCAP-01-0571 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PCAP-01-0571 ref|NP_001001618.1| chemokine (C-C motif) receptor 5 [Sus scrofa]... dbj|BAD08649.1| chemokine (C-C motif) receptor 5 [Sus scrofa] dbj|BAD08656.1| chemokine (C-C motif) receptor 5 [Sus scrofa...] dbj|BAD12135.1| chemokine C-C motif eceptor 5 [Sus scrofa] NP_001001618.1 1e-168 79% ...

  3. NCBI nr-aa BLAST: CBRC-ETEL-01-1350 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ETEL-01-1350 ref|NP_001001620.1| CC chemokine receptor 3 [Sus scrofa] dbj|BAD0...8659.1| chemokine (C-C motif) receptor 3 [Sus scrofa] dbj|BAD12132.1| chemokine C-C motif receptor 3 [Sus scrofa...] dbj|BAD12133.1| chemokine C-C motif receptor 3 [Sus scrofa] NP_001001620.1 1e-144 71% ...

  4. NCBI nr-aa BLAST: CBRC-CFAM-20-0021 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CFAM-20-0021 ref|NP_001001619.1| chemokine (C-C motif) receptor 2 [Sus scrofa]... dbj|BAD08648.1| chemokine (C-C motif) receptor 2 [Sus scrofa] dbj|BAD08655.1| chemokine (C-C motif) receptor 2 [Sus scrofa...] dbj|BAD12134.1| chemokine C-C motif receptor 2 [Sus scrofa] NP_001001619.1 1e-177 82% ...

  5. NCBI nr-aa BLAST: CBRC-EEUR-01-0018 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-EEUR-01-0018 ref|NP_001001620.1| CC chemokine receptor 3 [Sus scrofa] dbj|BAD0...8659.1| chemokine (C-C motif) receptor 3 [Sus scrofa] dbj|BAD12132.1| chemokine C-C motif receptor 3 [Sus scrofa...] dbj|BAD12133.1| chemokine C-C motif receptor 3 [Sus scrofa] NP_001001620.1 1e-105 75% ...

  6. NCBI nr-aa BLAST: CBRC-CPOR-01-1319 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CPOR-01-1319 ref|NP_001001619.1| chemokine (C-C motif) receptor 2 [Sus scrofa]... dbj|BAD08648.1| chemokine (C-C motif) receptor 2 [Sus scrofa] dbj|BAD08655.1| chemokine (C-C motif) receptor 2 [Sus scrofa...] dbj|BAD12134.1| chemokine C-C motif receptor 2 [Sus scrofa] NP_001001619.1 1e-154 77% ...

  7. NCBI nr-aa BLAST: CBRC-PVAM-01-1059 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PVAM-01-1059 ref|NP_001001619.1| chemokine (C-C motif) receptor 2 [Sus scrofa]... dbj|BAD08648.1| chemokine (C-C motif) receptor 2 [Sus scrofa] dbj|BAD08655.1| chemokine (C-C motif) receptor 2 [Sus scrofa...] dbj|BAD12134.1| chemokine C-C motif receptor 2 [Sus scrofa] NP_001001619.1 5e-40 69% ...

  8. NCBI nr-aa BLAST: CBRC-FCAT-01-1101 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-1101 ref|NP_001001532.1| chemokine (C-C motif) receptor 7 [Sus scrofa]... dbj|BAC57561.1| chemokine receptor 7 [Sus scrofa] dbj|BAC57929.1| chemokine receptor 7 [Sus scrofa] dbj|BAD...06309.1| chemokine (C-C motif) receptor 7 [Sus scrofa] NP_001001532.1 1e-145 94% ...

  9. NCBI nr-aa BLAST: CBRC-EEUR-01-0995 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-EEUR-01-0995 ref|NP_001001619.1| chemokine (C-C motif) receptor 2 [Sus scrofa]... dbj|BAD08648.1| chemokine (C-C motif) receptor 2 [Sus scrofa] dbj|BAD08655.1| chemokine (C-C motif) receptor 2 [Sus scrofa...] dbj|BAD12134.1| chemokine C-C motif receptor 2 [Sus scrofa] NP_001001619.1 3e-96 77% ...

  10. NCBI nr-aa BLAST: CBRC-GACU-05-0028 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GACU-05-0028 ref|NP_001001619.1| chemokine (C-C motif) receptor 2 [Sus scrofa]... dbj|BAD08648.1| chemokine (C-C motif) receptor 2 [Sus scrofa] dbj|BAD08655.1| chemokine (C-C motif) receptor 2 [Sus scrofa...] dbj|BAD12134.1| chemokine C-C motif receptor 2 [Sus scrofa] NP_001001619.1 2e-11 24% ...

  11. NCBI nr-aa BLAST: CBRC-VPAC-01-1268 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-VPAC-01-1268 ref|NP_001001618.1| chemokine (C-C motif) receptor 5 [Sus scrofa]... dbj|BAD08649.1| chemokine (C-C motif) receptor 5 [Sus scrofa] dbj|BAD08656.1| chemokine (C-C motif) receptor 5 [Sus scrofa...] dbj|BAD12135.1| chemokine C-C motif eceptor 5 [Sus scrofa] NP_001001618.1 1e-151 76% ...

  12. NCBI nr-aa BLAST: CBRC-ETEL-01-0875 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ETEL-01-0875 ref|NP_001001618.1| chemokine (C-C motif) receptor 5 [Sus scrofa]... dbj|BAD08649.1| chemokine (C-C motif) receptor 5 [Sus scrofa] dbj|BAD08656.1| chemokine (C-C motif) receptor 5 [Sus scrofa...] dbj|BAD12135.1| chemokine C-C motif eceptor 5 [Sus scrofa] NP_001001618.1 1e-155 74% ...

  13. NCBI nr-aa BLAST: CBRC-TGUT-04-0033 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TGUT-04-0033 ref|NP_001001618.1| chemokine (C-C motif) receptor 5 [Sus scrofa]... dbj|BAD08649.1| chemokine (C-C motif) receptor 5 [Sus scrofa] dbj|BAD08656.1| chemokine (C-C motif) receptor 5 [Sus scrofa...] dbj|BAD12135.1| chemokine C-C motif eceptor 5 [Sus scrofa] NP_001001618.1 1e-128 62% ...

  14. NCBI nr-aa BLAST: CBRC-MEUG-01-0804 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MEUG-01-0804 ref|NP_001001618.1| chemokine (C-C motif) receptor 5 [Sus scrofa]... dbj|BAD08649.1| chemokine (C-C motif) receptor 5 [Sus scrofa] dbj|BAD08656.1| chemokine (C-C motif) receptor 5 [Sus scrofa...] dbj|BAD12135.1| chemokine C-C motif eceptor 5 [Sus scrofa] NP_001001618.1 1e-159 77% ...

  15. NCBI nr-aa BLAST: CBRC-MMUS-09-0220 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-09-0220 ref|NP_001001619.1| chemokine (C-C motif) receptor 2 [Sus scrofa]... dbj|BAD08648.1| chemokine (C-C motif) receptor 2 [Sus scrofa] dbj|BAD08655.1| chemokine (C-C motif) receptor 2 [Sus scrofa...] dbj|BAD12134.1| chemokine C-C motif receptor 2 [Sus scrofa] NP_001001619.1 1e-171 77% ...

  16. NCBI nr-aa BLAST: CBRC-DNOV-01-0440 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-0440 ref|NP_001001619.1| chemokine (C-C motif) receptor 2 [Sus scrofa]... dbj|BAD08648.1| chemokine (C-C motif) receptor 2 [Sus scrofa] dbj|BAD08655.1| chemokine (C-C motif) receptor 2 [Sus scrofa...] dbj|BAD12134.1| chemokine C-C motif receptor 2 [Sus scrofa] NP_001001619.1 1e-156 72% ...

  17. NCBI nr-aa BLAST: CBRC-DNOV-01-2689 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-2689 ref|NP_001001532.1| chemokine (C-C motif) receptor 7 [Sus scrofa]... dbj|BAC57561.1| chemokine receptor 7 [Sus scrofa] dbj|BAC57929.1| chemokine receptor 7 [Sus scrofa] dbj|BAD...06309.1| chemokine (C-C motif) receptor 7 [Sus scrofa] NP_001001532.1 4e-76 89% ...

  18. NCBI nr-aa BLAST: CBRC-ETEL-01-1529 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-ETEL-01-1529 ref|NP_001001532.1| chemokine (C-C motif) receptor 7 [Sus scrofa]... dbj|BAC57561.1| chemokine receptor 7 [Sus scrofa] dbj|BAC57929.1| chemokine receptor 7 [Sus scrofa] dbj|BAD...06309.1| chemokine (C-C motif) receptor 7 [Sus scrofa] NP_001001532.1 0.0 89% ...

  19. NCBI nr-aa BLAST: CBRC-OLAT-24-0006 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OLAT-24-0006 ref|NP_001001619.1| chemokine (C-C motif) receptor 2 [Sus scrofa]... dbj|BAD08648.1| chemokine (C-C motif) receptor 2 [Sus scrofa] dbj|BAD08655.1| chemokine (C-C motif) receptor 2 [Sus scrofa...] dbj|BAD12134.1| chemokine C-C motif receptor 2 [Sus scrofa] NP_001001619.1 1e-24 25% ...

  20. NCBI nr-aa BLAST: CBRC-PTRO-04-0020 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PTRO-04-0020 ref|NP_001001619.1| chemokine (C-C motif) receptor 2 [Sus scrofa]... dbj|BAD08648.1| chemokine (C-C motif) receptor 2 [Sus scrofa] dbj|BAD08655.1| chemokine (C-C motif) receptor 2 [Sus scrofa...] dbj|BAD12134.1| chemokine C-C motif receptor 2 [Sus scrofa] NP_001001619.1 1e-144 76% ...

  1. NCBI nr-aa BLAST: CBRC-RMAC-16-0027 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RMAC-16-0027 ref|NP_001001532.1| chemokine (C-C motif) receptor 7 [Sus scrofa]... dbj|BAC57561.1| chemokine receptor 7 [Sus scrofa] dbj|BAC57929.1| chemokine receptor 7 [Sus scrofa] dbj|BAD...06309.1| chemokine (C-C motif) receptor 7 [Sus scrofa] NP_001001532.1 0.0 92% ...

  2. NCBI nr-aa BLAST: CBRC-TBEL-01-2403 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TBEL-01-2403 ref|NP_001001619.1| chemokine (C-C motif) receptor 2 [Sus scrofa]... dbj|BAD08648.1| chemokine (C-C motif) receptor 2 [Sus scrofa] dbj|BAD08655.1| chemokine (C-C motif) receptor 2 [Sus scrofa...] dbj|BAD12134.1| chemokine C-C motif receptor 2 [Sus scrofa] NP_001001619.1 1e-158 75% ...

  3. NCBI nr-aa BLAST: CBRC-SARA-01-0123 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-SARA-01-0123 dbj|BAF32759.1| taste receptor, type 1, member 3 [Sus scrofa domestic...a] dbj|BAF32761.1| taste receptor, type 1, member 3 [Sus scrofa domestica] dbj|BAF32762.1| taste recepto...r, type 1, member 3 [Sus scrofa domestica] dbj|BAF32763.1| taste receptor, type 1, member 3 [Sus scrofa domestica] BAF32759.1 1e-120 70% ...

  4. NCBI nr-aa BLAST: CBRC-EEUR-01-0679 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-EEUR-01-0679 dbj|BAF32759.1| taste receptor, type 1, member 3 [Sus scrofa domestic...a] dbj|BAF32761.1| taste receptor, type 1, member 3 [Sus scrofa domestica] dbj|BAF32762.1| taste recepto...r, type 1, member 3 [Sus scrofa domestica] dbj|BAF32763.1| taste receptor, type 1, member 3 [Sus scrofa domestica] BAF32759.1 1e-173 77% ...

  5. NCBI nr-aa BLAST: CBRC-DNOV-01-1921 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-1921 dbj|BAF32759.1| taste receptor, type 1, member 3 [Sus scrofa domestic...a] dbj|BAF32761.1| taste receptor, type 1, member 3 [Sus scrofa domestica] dbj|BAF32762.1| taste recepto...r, type 1, member 3 [Sus scrofa domestica] dbj|BAF32763.1| taste receptor, type 1, member 3 [Sus scrofa domestica] BAF32759.1 1e-123 55% ...

  6. NCBI nr-aa BLAST: CBRC-EEUR-01-1404 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-EEUR-01-1404 dbj|BAF32759.1| taste receptor, type 1, member 3 [Sus scrofa domestic...a] dbj|BAF32761.1| taste receptor, type 1, member 3 [Sus scrofa domestica] dbj|BAF32762.1| taste recepto...r, type 1, member 3 [Sus scrofa domestica] dbj|BAF32763.1| taste receptor, type 1, member 3 [Sus scrofa domestica] BAF32759.1 3e-89 71% ...

  7. NCBI nr-aa BLAST: CBRC-BTAU-01-2354 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-BTAU-01-2354 dbj|BAF32759.1| taste receptor, type 1, member 3 [Sus scrofa domestic...a] dbj|BAF32761.1| taste receptor, type 1, member 3 [Sus scrofa domestica] dbj|BAF32762.1| taste recepto...r, type 1, member 3 [Sus scrofa domestica] dbj|BAF32763.1| taste receptor, type 1, member 3 [Sus scrofa domestica] BAF32759.1 0.0 77% ...

  8. NCBI nr-aa BLAST: CBRC-CPOR-01-1833 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CPOR-01-1833 dbj|BAF32759.1| taste receptor, type 1, member 3 [Sus scrofa domestic...a] dbj|BAF32761.1| taste receptor, type 1, member 3 [Sus scrofa domestica] dbj|BAF32762.1| taste recepto...r, type 1, member 3 [Sus scrofa domestica] dbj|BAF32763.1| taste receptor, type 1, member 3 [Sus scrofa domestica] BAF32759.1 2e-93 56% ...

  9. NCBI nr-aa BLAST: CBRC-CFAM-05-0060 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CFAM-05-0060 dbj|BAF32759.1| taste receptor, type 1, member 3 [Sus scrofa domestic...a] dbj|BAF32761.1| taste receptor, type 1, member 3 [Sus scrofa domestica] dbj|BAF32762.1| taste recepto...r, type 1, member 3 [Sus scrofa domestica] dbj|BAF32763.1| taste receptor, type 1, member 3 [Sus scrofa domestica] BAF32759.1 0.0 82% ...

  10. NCBI nr-aa BLAST: CBRC-FCAT-01-0620 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-FCAT-01-0620 dbj|BAF32759.1| taste receptor, type 1, member 3 [Sus scrofa domestic...a] dbj|BAF32761.1| taste receptor, type 1, member 3 [Sus scrofa domestica] dbj|BAF32762.1| taste recepto...r, type 1, member 3 [Sus scrofa domestica] dbj|BAF32763.1| taste receptor, type 1, member 3 [Sus scrofa domestica] BAF32759.1 0.0 76% ...

  11. NCBI nr-aa BLAST: CBRC-SARA-01-0466 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-SARA-01-0466 dbj|BAF32759.1| taste receptor, type 1, member 3 [Sus scrofa domestic...a] dbj|BAF32761.1| taste receptor, type 1, member 3 [Sus scrofa domestica] dbj|BAF32762.1| taste recepto...r, type 1, member 3 [Sus scrofa domestica] dbj|BAF32763.1| taste receptor, type 1, member 3 [Sus scrofa domestica] BAF32759.1 3e-32 71% ...

  12. Santa Teresa y sus cartas, historia de los sentimientos

    Directory of Open Access Journals (Sweden)

    Egido, Teófanes

    2015-12-01

    Full Text Available Historical reflection on a peculiar dimension of St. Teresa of Jesus: the expression of her feelings in her writings, particularly in her abundant letters. The article focuses on the sense of humor, the joyfulness, and the importance of laughter in St. Teresa language, and also on the feeling of endearment with her family, with her order, with fray Juan de la Cruz. Ample space is dedicated to the tenderness towards girls in her convents. St. Teresa of Jesus appears as transgressor of 16th century social behaviours.Reflexión histórica sobre una dimensión peculiar de santa Teresa de Jesús: la expresión de sus sentimientos en sus escritos, de forma más especial en sus cartas abundantes. El artículo se centra en el sentido del humor, de la alegría, en la importancia de la risa en el lenguaje de santa Teresa y en el sentimiento de ternura con su familia, con su orden, con fray Juan de la Cruz. Se dedica un espacio amplio a la ternura hacia las niñas en sus conventos. Aparece santa Teresa de Jesús como trasgresora de los comportamientos sociales del siglo XVI.

  13. Identificación de cepas de salmonella spp resistentes a antimicrobianos, y factores de riesgo para su circulación, en aves y cerdos mantenidos en sistemas productivos de traspatio de la región del libertador general Bernardo O'Higgins, Chile

    OpenAIRE

    Gómez Vega, Elizabeth

    2013-01-01

    El actual aumento de la incidencia de Salmonella spp, ha provocado un gran impacto, tanto en la salud pública como en salud animal. Se considera que el incremento de la diseminación de los microorganismos a través de las cadenas productivas animales (bovinos, cerdos, pollos y en especial gallinas ponedoras) tiene un rol fundamental en este evento. Los principales reservorios de Salmonella spp son animales portadores asintomáticos y las fuentes de infección más frecuente son los alimentos o...

  14. Evaluación de la respuesta postvacunal a peste porcina clásica por medio de diferentes pruebas diagnósticas en cerdos desafiados experimentalmente / Assessment of response to classical swine fever following immunization by different diagnostic tests in experimentally challenged pigs.

    OpenAIRE

    Piñeros Duque, Ricardo Javier

    2011-01-01

    El propósito de este trabajo fue evaluar dos vacunas comerciales de Peste Porcina Clásica (PPC) utilizadas en Colombia elaboradas con la cepa China. El diseño experimental consistió en un grupo de treinta (30) cerdos provenientes de una explotación intensiva tecnificada convencional, de 50 días de edad en promedio, no vacunados a PPC, con una distribución homogénea de sexos, lo cual permitió la conformación de tres grupos: 12 animales para el grupo ¨A¨, 12 animales para el grupo ¨B¨ ...

  15. Estudio histomorfológico de la capacidad de regeneración ósea del plasma rico en plaquetas, médula ósea y fosfato tricálcico: estudio experimental en cerdos “regeneración ósea en cirugía experimental en cerdos”

    OpenAIRE

    Castañeda Vega, Paul; López López, José, 1958-; Chimenos Küstner, Eduardo; Manzanares Céspedes, Cristina; Muñoz Sánchez, Juan; Jané Salas, Enric; Álvarez López, José; Gimeno Sanding, Alvaro

    2010-01-01

    Objetivo: Se valora histomorfológicamente la capacidad de regeneración ósea del plasma rico en plaquetas, a distintas concentraciones, y el extracto de médula ósea rico en plaquetas, en comparación con β-fosfato tricálcico. Material y Metodo: Se realiza un trabajo experimental en 8 cerdos, a los que se les practican trepanaciones mandibulares para colocar los materiales a estudio. Las muestras obtenidas se observan mediante microscopio electrónico y se realizan fotografías sistemá...

  16. NCBI nr-aa BLAST: CBRC-MMUS-10-0067 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUS-10-0067 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 0.0 84% ...

  17. NCBI nr-aa BLAST: CBRC-MLUC-01-0476 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MLUC-01-0476 ref|NP_001116609.1| melanocortin 3 receptor [Sus scrofa] emb|CAN1...3145.1| melanocortin 3 receptor [Sus scrofa] gb|ABU62784.1| melanocortin-3 receptor [Sus scrofa] NP_001116609.1 1e-107 64% ...

  18. NCBI nr-aa BLAST: CBRC-SARA-01-0253 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-SARA-01-0253 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 3e-51 67% ...

  19. NCBI nr-aa BLAST: CBRC-OGAR-01-0646 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OGAR-01-0646 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 9e-63 60% ...

  20. NCBI nr-aa BLAST: CBRC-PTRO-20-0002 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PTRO-20-0002 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 1e-175 76% ...

  1. NCBI nr-aa BLAST: CBRC-CFAM-20-0127 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-CFAM-20-0127 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 1e-164 77% ...

  2. NCBI nr-aa BLAST: CBRC-HSAP-19-0005 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-HSAP-19-0005 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 0.0 86% ...

  3. NCBI nr-aa BLAST: CBRC-PMAR-01-0754 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PMAR-01-0754 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 1e-100 55% ...

  4. NCBI nr-aa BLAST: CBRC-BTAU-01-1994 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-BTAU-01-1994 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 0.0 83% ...

  5. NCBI nr-aa BLAST: CBRC-TTRU-01-0593 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0593 ref|NP_001116572.1| chemokine-like receptor 1 [Sus scrofa] gb|ACA...42765.1| chemokine-like receptor 1 [Sus scrofa] gb|ACD31687.1| chemokine-like receptor 1 [Sus scrofa] NP_001116572.1 0.0 92% ...

  6. NCBI nr-aa BLAST: CBRC-VPAC-01-0595 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-VPAC-01-0595 ref|NP_001116674.1| Toll-like receptor 5 [Sus scrofa] dbj|BAD9180...0.1| Toll-like receptor 5 [Sus scrofa] gb|ACN71220.1| toll-like receptor 5 [Sus scrofa] NP_001116674.1 0.0 83% ...

  7. NCBI nr-aa BLAST: CBRC-VPAC-01-1573 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-VPAC-01-1573 ref|NP_999519.1| calcitonin receptor [Sus scrofa] gb|AAA31023.1| ...calcitonin receptor [Sus scrofa] emb|CAA83232.1| calcitonin receptor-1a [Sus scrofa] NP_999519.1 1e-152 79% ...

  8. NCBI nr-aa BLAST: CBRC-VPAC-01-1099 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-VPAC-01-1099 ref|NP_001116572.1| chemokine-like receptor 1 [Sus scrofa] gb|ACA...42765.1| chemokine-like receptor 1 [Sus scrofa] gb|ACD31687.1| chemokine-like receptor 1 [Sus scrofa] NP_001116572.1 4e-98 85% ...

  9. NCBI nr-aa BLAST: CBRC-PVAM-01-1006 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PVAM-01-1006 ref|NP_001116572.1| chemokine-like receptor 1 [Sus scrofa] gb|ACA...42765.1| chemokine-like receptor 1 [Sus scrofa] gb|ACD31687.1| chemokine-like receptor 1 [Sus scrofa] NP_001116572.1 0.0 84% ...

  10. NCBI nr-aa BLAST: CBRC-TSYR-01-0189 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TSYR-01-0189 ref|NP_001116609.1| melanocortin 3 receptor [Sus scrofa] emb|CAN1...3145.1| melanocortin 3 receptor [Sus scrofa] gb|ABU62784.1| melanocortin-3 receptor [Sus scrofa] NP_001116609.1 2e-86 86% ...

  11. NCBI nr-aa BLAST: CBRC-PABE-20-0001 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PABE-20-0001 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 1e-159 73% ...

  12. NCBI nr-aa BLAST: CBRC-RNOR-07-0201 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RNOR-07-0201 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 0.0 83% ...

  13. NCBI nr-aa BLAST: CBRC-PVAM-01-0946 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-PVAM-01-0946 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 0.0 83% ...

  14. NCBI nr-aa BLAST: CBRC-MMUR-01-0983 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MMUR-01-0983 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 9e-91 71% ...

  15. NCBI nr-aa BLAST: CBRC-TTRU-01-0910 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-TTRU-01-0910 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 0.0 89% ...

  16. NCBI nr-aa BLAST: CBRC-OANA-01-1928 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OANA-01-1928 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 1e-120 63% ...

  17. NCBI nr-aa BLAST: CBRC-OPRI-01-0091 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-OPRI-01-0091 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 5e-58 54% ...

  18. NCBI nr-aa BLAST: CBRC-RMAC-19-0001 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-RMAC-19-0001 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 1e-127 63% ...

  19. NCBI nr-aa BLAST: CBRC-MLUC-01-0822 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-MLUC-01-0822 ref|NP_001038089.1| G protein-coupled receptor 54 [Sus scrofa] gb...|ABE73452.1| G protein-coupled receptor 54 [Sus scrofa] gb|ABE73453.1| G protein-coupled receptor 54 [Sus scrofa] NP_001038089.1 1e-180 84% ...

  20. NCBI nr-aa BLAST: CBRC-DNOV-01-0663 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-DNOV-01-0663 ref|NP_001090897.1| interferon regulatory factor 7 [Sus scrofa] g...b|ABM69124.1| interferon regulatory factor 7 [Sus scrofa] dbj|BAF42541.2| interferon regulatory factor 7 [Sus scrofa] NP_001090897.1 2e-51 49% ...

  1. Bienestar Animal: Comportamiento productivo y salud de las crías de cerdos alojadas en tecnología Flat Deck - Animal Well-being: Productive behaviour and health of the breeding of pigs housed in technology Flat Deck

    Directory of Open Access Journals (Sweden)

    Maikel Díaz Gutierrez

    2010-08-01

    Full Text Available ResumenCon el objetivo de evaluar comportamiento productivo y de salud de las crías de cerdos alojados en tecnología Flat Deck se realiza el trabajo Bienestar Animal: Comportamiento productivo y de salud de las crías de cerdos alojadas en tecnología Flat Deck. Para ello se utilizaron 24 bóxer de maternidad de tecnología Flat Deck, de estos 12 de origen chino y 12 de origen español con características similares. Se analizaron un total de 1433 crías mestizas (cruce de hembras Yorkland entre la tercera y cuarta paridad con machos CC-21, L-35 y Duroc. De estas, 715 corresponden a la tecnología de origen chino y 718 a la tecnología de origen español, alojadas aproximadamente a 10 crías por bóxer. Se tuvo en cuenta el tamaño de la camada al nacer (TCN y peso al nacer (PN, peso al destete(PD y ganancia media diaria (GMD; además de los indicadores de salud(enfermos y muertes por tecnología. En los indicadores evaluadosinicialmente que no son influenciados por las tecnologías no sepresentaron diferencias estadísticamente significativas (p>0,05. En los indicadores productivos por tecnología no se encontraron diferencia estadísticamente significativa, encontrándose las medias del peso al destete entre 7.08 y 7.15 y una ganancia media diaria de 219.11 y 221.09 respectivamente. No siendo así para los indicadores de salud, quien fue desfavorable para la tecnología de origen chino donde se presentaron 27 animales enfermos por encima de los enfermos en la tecnología de origen español.SummaryWith the objective of evaluating productive behaviour and of health of the breeding of pigs housed in technology Flat Deck he/she is carried out the work Animal Well-being: Productive behaviour and of health of the breeding of pigs housed in technology Flat Deck. For they were used it 24 boxers of technology maternity Flat Deck, of these 12 of Chinese origin and 12 of Spanish origin with characteristic similar. They were analyzed a total of 1433

  2. Detección mediante RT-PCR en tiempo real del virus vacunal en cerdos inmunizados con la vacuna cubana contra la peste porcina clásica

    Directory of Open Access Journals (Sweden)

    Tania Campos-Cuello

    2016-08-01

    Full Text Available La peste porcina clásica (PPC es una enfermedad viral infectocontagiosa, producida por un virus ARN del género Pestivirus, familia Flaviviridae. En la actualidad es una de las causas de pérdidas económicas en la industria porcina a nivel mundial. En su prevención se han utilizado vacunas vivas atenuadas, empleando la cepa China lapinizada. La Reacción en Cadena de la Polimerasa Reverso Transcriptasa (RT-PCR ha sido uno de los métodos más sensibles aplicado en Medicina Veterinaria para la detección de virus ARN. En el caso del virus de la PPC es muy útil porque el ácido nucleico se puede detectar desde muy temprano en la infección y en periodos más largos en aquellos animales que se recuperan. El objetivo de este estudio fue aplicar la técnica de RT-PCR en tiempo real para la detección de la cepa China lapinizada de la vacuna cubana contra la PPC. Las tonsilas de los cerdos vacunados fueron el órgano más positivo en la detección del ARN del virus vacunal. Los resultados obtenidos evidenciaron una interferencia del virus vacunal en el diagnóstico, siendo el día 12 posvacunación en el que se obtiene una emisión umbral de fluorescencia más bajo.

  3. El tren de alta velocidad y sus efectos espaciales

    Directory of Open Access Journals (Sweden)

    Javier Gutiérrez Puebla

    2004-01-01

    Full Text Available Este trabajo aporta datos, reflexiones e ideas sobre el tren de alta velocidad desde una perspectiva espacial. Se estudian las características básicas de este nuevo modo de transporte, la evolución de las redes en los países pioneros y la conformación de la red transeuropea de alta velocidad ferroviaria. Asimismo se analizan el proceso de contracción del espacio y sus consecuencias sobre la competitividad de las ciudades y la cohesión territorial; el efecto túnel y sus consecuencias polarizadoras sobre el territorio; los impactos de la alta velocidad sobre la movilidad, al captar demanda de otros modos de transporte y generar una demanda nueva; y los impactos en la ciudad (conformación de áreas metropolitanas discontinuas y transformaciones en el entorno de las estaciones.

  4. NETWORKS OF HEALTH CARE: A CHALLENGE TO SUS MANAGEMENT

    Directory of Open Access Journals (Sweden)

    Camila Dubow

    2013-09-01

    Full Text Available The article proposes a critical reflection, based on national law, scholarly, scientific, on the current development of Networks of Health Care, as a strategy for strengthening the Single Health System (SUS. Are weighted inefficiency of traditional ways of organizing care and management, the challenge of Network Health Care for comprehensive care and management mechanisms used in this process. The work provides subsidies for the care practices and health management are reflected, pointing strategies that result in disruptions of paradigms through a refocusing of attention in existing models. For networks of health care can be consolidated, is fundamental to political sensitivity of health managers with a commitment to build a new model of care, through the struggle to consolidate the SUS and the realization of the principles of universality, comprehensiveness and equity.

  5. Quince Duncan y sus aportes a la literatura costarricense

    OpenAIRE

    2016-01-01

    Este ensayo hace un recorrido por la obra de Quince Duncan, escritor afrocostarricense, y la importancia de sus cuentos y novelas para la literatura producida en Costa Rica en los últimos treinta y cinco años.This paper looks through the literary work of Quince Duncan, an AfroCosta Rican writer, and the importance of his short stories and novels to the literature produced in Costa Rica during the last thirty-five years.

  6. Material test data of SUS304 welded joints

    Energy Technology Data Exchange (ETDEWEB)

    Asayama, Tai [Japan Nuclear Cycle Development Inst., Oarai, Ibaraki (Japan). Oarai Engineering Center; Kawakami, Tomohiro [Nuclear Energy System Incorporation, Tokyo (Japan)

    1999-10-01

    This report summarizes the material test data of SUS304 welded joints. Numbers of the data are as follows: Tensile tests 71 (Post-irradiation: 39, Others: 32), Creep tests 77 (Post-irradiation: 20, Others: 57), Fatigue tests 50 (Post-irradiation: 0), Creep-fatigue tests 14 (Post-irradiation: 0). This report consists of the printouts from 'the structural material data processing system'. (author)

  7. Las comunidades y sus revistas científicas

    Directory of Open Access Journals (Sweden)

    Leonardo Romero

    2014-03-01

    Full Text Available Las investigaciones realizadas y expuestas ante las sociedades científicas, tuvieron en las revistas científicas un medio ex professode comunicación y diseminación de sus memorias y descubrimientos. Sin embargo en el escenario actual los investigadores y las revistas científicas adoptan nuevas características y se ven influenciadas por diferentes tendencias que requieren una estrategia de desarrollo editorial adecuada.

  8. NCBI nr-aa BLAST: CBRC-GGAL-20-0005 [SEVENS

    Lifescience Database Archive (English)

    Full Text Available CBRC-GGAL-20-0005 ref|NP_001008687.1| prion protein [Sus scrofa] sp|P49927|PRIO_PIG Major prion... protein precursor (PrP) (CD230 antigen) gb|AAA92862.1| prion protein gb|AAV83492.1| prion prote...in [Sus scrofa] gb|AAV83495.1| prion protein [Sus scrofa] gb|ABL75507.1| prion protein [Sus scrofa] gb|ABO21764.1| prion protein [Sus scrofa] NP_001008687.1 1.3 27% ...

  9. LA PSICOLOGÍA POSITIVA Y SUS AMIGOS: EN EVIDENCIA

    OpenAIRE

    Marino Pérez-Álvarez

    2013-01-01

    La Psicología Positiva está en debate. Ha recibido importantes críticas, a las que reaccionan sus defensores, como es natural. El presente artículo trata, más que nada, de establecer las razones de una crítica dirigida a los fundamentos mismos de la Psicología Positiva: la positividad y la cientificidad. Frente al marcado énfasis en los beneficios de los afectos positivos, los estudios muestran que los afectos negativos pueden ser tan positivos, como negativos pueden ser los afectos positivos...

  10. LA CRISIS FINANCIERA EN ESTADOS UNIDOS Y SUS CONSECUENCIAS INTERNACIONALES

    OpenAIRE

    2009-01-01

    El presente artículo considera la crisis internacional basada en el sistema financiero estadounidense desde una perspectiva analítica que trata de unir tres aspectos íntimamente ligados en la generación y el desenlace de la misma, 1) La naturaleza de la crisis situada esencialmente en el sistema financiero de Estados Unidos, su relación con la especulación inmobiliaria y sus consecuencias internacionales, 2) El deterioro preexistente de la competitividad sistémica y la hegemonía de Estados Un...

  11. La ciudad islámica: sus referencias culturales

    OpenAIRE

    Nakhli Mtiri, AbdelKader

    2006-01-01

    La ciudad islámica o llamada la-medina, siempre ha sido criticada por su forma de ser al compararla con la ciudad romana o griega. Pero este espacio urbano es el resultado de una civilización conocida en el tiempo y en el espacio. Tiene sus propias referencias culturales como filosóficas. Su estructura urbana ha sido compatible con las creencias y las perspectivas sociológicas, económicas y políticas de la sociedad musulmana. Es una imagen de marca. [ABSTRACT]The Islamic city, or what is ca...

  12. In vivo body composition in autochthonous and conventional pig breeding groups by dual-energy X-ray absorptiometry and magnetic resonance imaging under special consideration of Cerdo Ibérico.

    Science.gov (United States)

    Kremer, P V; Fernández-Fígares, I; Förster, M; Scholz, A M

    2012-12-01

    The improvement of carcass quality is one of the main breeding goals in pig production. To select appropriate breeding animals, it is of major concern to exactly and reliably analyze the body composition in vivo. Therefore, the objective of the study was to examine whether the combination of dual-energy X-ray absorptiometry (DXA) and magnetic resonance imaging (MRI) offers the opportunity to reliably analyze quantitative and qualitative body composition characteristics of different pig breeding groups in vivo. In this study, a total of 77 pigs were studied by DXA and MRI at an average age of 154 days. The pigs originated from different autochthonous or conventional breeds or crossbreeds and were grouped into six breed types: Cerdo Ibérico (Ib); Duroc × Ib (Du_Ib); White Sow Lines (WSL, including German Landrace and German Large White); Hampshire/Pietrain (Pi_Ha, including Hampshire, Pietrain × Hampshire (PiHa) and Pietrain × PiHa); Pietrain/Duroc (Pi_Du, including Pietrain × Duroc (PiDu) and Pietrain × PiDu); crossbred WSL (PiDu_WSL, including Pietrain × WSL and PiDu × WSL). A whole-body scan was performed by DXA with a GE Lunar DPX-IQ in order to measure the amount and percentage of fat tissue (FM; %FM), lean tissue (LM; %LM) and bone mineral, whereas a Siemens Magnetom Open with a large body coil was used for MRI in the thorax region between 13th and 14th vertebrae in order to measure the area of the loin (LA) and the above back fat area (FA) of both body sides. A GLM procedure using SAS 9.2 was used to analyze the data. As expected, the native breed Ib followed by Du_Ib crossbreeds showed the highest %FM (27.2%, 25.0%) combined with the smallest LA (46.2 cm2, 73.6 cm2), whereas Ib had the lowest BW at an average age of 154 days. Pigs with Pi_Ha origin presented the least %FM (12.4%) and largest LA (99.5 cm2). The WSL and PiDu_WSL showed an intermediate body composition. Therefore, it could be concluded that DXA and MRI and especially their combination

  13. Entusiastas, aficionados y espectadores: sus valores, motivaciones y compromiso

    Directory of Open Access Journals (Sweden)

    Miquel Torregrosa

    2009-01-01

    Full Text Available El interés de las ciencias aplicadas al deporte en los espectadores y el espectáculo deportivo ha sido más bien escaso y en muchos casos sobrecentrado en la violencia. El objetivo de este trabajo es proponer una clasificación alternativa de los aficionados al fútbol a partir del tipo de contacto que tienen con el espectáculo y describir sus valores, motivaciones y compromiso. Mil setecientas diecinueve personas asistentes a partidos de fútbol organizados por la Liga de Fútbol Profesional contestaron cuestionarios sobre sus valores, motivaciones y relación con el fútbol. Los resultados muestranque los entusiastas son los aficionados más motivados y comprometidos con el fútbol al mismo tiempo que participan significativamente más de valores relacionados con el conservadurismo y la autopromoción que las otras tres categorías (espectadores, aficionados presenciales y aficionados virtuales. La clasificaciónpropuesta en base al tipo de contacto con el fútbol permite conceptualizar el fútbol como algo más que lo que ocurre en los estadios y abre las puertas a intervencionesdesde lo positivo para promover el fair play y la deportividad en los aficionados.

  14. LA PSICOLOGÍA POSITIVA Y SUS AMIGOS: EN EVIDENCIA

    Directory of Open Access Journals (Sweden)

    Marino Pérez-Álvarez

    2013-09-01

    Full Text Available La Psicología Positiva está en debate. Ha recibido importantes críticas, a las que reaccionan sus defensores, como es natural. El presente artículo trata, más que nada, de establecer las razones de una crítica dirigida a los fundamentos mismos de la Psicología Positiva: la positividad y la cientificidad. Frente al marcado énfasis en los beneficios de los afectos positivos, los estudios muestran que los afectos negativos pueden ser tan positivos, como negativos pueden ser los afectos positivos. La positividad no está del lado positivo (porque no hay tal lado. Tocante a lo que la Psicología Positiva dice saber científicamente de la felicidad, no parece ser nada que no se supiera. Incluso las mejores teorías, como el modelo de actividad positiva y la teoría de la extensión-y-construcción impresionan por sus trivialidades según están plagadas de tautologías. Notable es también la falacia demostrada de la ratio de positividad. Finalmente, se plantea que la felicidad no es sostenible como principio de vida (su búsqueda no es universal ni lo mejor que hacer en la vida, ni como objeto científico, como no sea acosta de hipostasiar la experiencia subjetiva.

  15. Cicatrici: El secreto de sus ojos di Sacheri e Campanella

    Directory of Open Access Journals (Sweden)

    Sandro Volpe

    2012-12-01

    Full Text Available In 2005 the Argentinian writer Eduardo Sacheri published The Question in Their Eyes (La pregunta de sus ojos.  The novel narrates on a double level, both fictional and temporal, the decision of a retired prosecutor to come to terms with a case of rape and murder which had taken place years earlier: his life had been shattered by the consequences of that tragedy, and writing about it would be a way to start living again.  The director Juan José Campanella - widely famous for having directed episodes of the television series Law & Order, but especially as the director of successful films such as El hijo de la novia - decided to adapt the novel and involved Sacheri in the project: the close collaboration between writer and director produced a bold screenplay that adds to the noir thriller a more romantic dimension.  Disproving the commonplace of the writer jealous of his work, they wrote together a new version which retains certain elements of the original text, but also introduces a number of relevant changes to the structure, characters and ending of the novel.  The Secret in Their Eyes  (El secreto de sus ojos, shot in 2008, was released the following year and in 2010 won the Academy Award for best foreign language film.

  16. Valoración de parámetros clínicos y lesiones en órganos de cerdos durante el período posdestete Evaluation of clinical parameters and lesions in pig organs during post-weaning period

    Directory of Open Access Journals (Sweden)

    Julián Mejía-Medina

    2012-01-01

    Full Text Available El destete de cerdos está asociado con múltiples factores que generan respuestas inflamatorias tempranas en órganos internos y alta incidencia de diarreas. En el Centro de Investigación San Pablo de la Universidad Nacional de Colombia sede Medellín, se evaluaron los parámetros clínicos y las lesiones en órganos internos en dieciséis cerdos destetados a veintiún días de edad, que fueron alimentados durante diez días con una dieta a base de leche. Cada uno, cinco, siete y diez días posdestete, se sacrificaron cuatro cerdos y se tomaron para estudio muestras de intestino delgado, estómago, hígado, páncreas, corazón, pulmones, riñones y bazo. Las lesiones determinadas fueron congestión, edema, y hemorragia; se asignó un valor según el grado de presentación: ausente (0, leve (1, leve-moderada (2, moderada-severa (3, severa (4. Los animales fueron pesados al destete y en el momento de sacrificio. Se encontraron diferencias (P To evaluate the effect of early weaning on clinical parameters, development and occurrence of lesions in organs of systemic importance, and weight gain in pigs evaluations were carried out. The experiment was conducted in the San Pablo Production Research Center of the Universidad Nacional de Colombia (Medellín. We used 16 weaned pigs at 21 days of age. The animals were fed for 10 days with a basal diet (milk. Four pigs were slaughtered on days 1, 5, 7 and 10 post-weaning and samples of intestine, stomach, liver, pancreas, heart, lungs, kidneys and spleen were extracted. Congestion, edema, and hemorrhage were the lesions determined; a value according to the degree of presentation was assigned: absent (0, mild (1, mild-moderate (2, moderate-severe (3, severe (4. The animals were weighed on weaning day, and the day of slaughter. Statistical difference (P < 0.01 was found in macroscopic appearance of lesions, organ weight, rectal temperature, and weight gain. On the first day of post-weaning the highest values

  17. Características hematológicas y patológicas de cerdos inoculados experimentalmente con el aislado chileno del virus síndrome respiratorio y reproductivo porcino Haematological and pathological findings of pigs experimentally inoculated with a Chilean isolate of porcine reproductive and respiratory syndrome virus

    Directory of Open Access Journals (Sweden)

    E Ramírez

    2006-01-01

    Full Text Available El presente trabajo se planteó con el fin de caracterizar las manifestaciones hematológicas y lesiones de cerdos inoculados con el aislado chileno del vPRRS. Se utilizaron 12 cerdos de 3 semanas de edad divididos en 4 grupos de 3 animales cada uno, uno de los cuales correspondió al grupo control que fueron sacrificados a los 0 días postinoculación (dpi, y los 3 grupos restantes a los cerdos inoculados que fueron sacrificados a los 7, 14 y 21 dpi. Durante cada muestreo se recolectó sangre para hemogramas y al sacrificio se determinaron las lesiones macroscópicas y se tomaron muestras para frotis de médula ósea y para histopatología. Los resultados de este estudio revelaron alteraciones hematológicas caracterizadas por descenso del volumen globular (PThe aims of this study were to characterize the haematological and bone marrow changes, gross and microscopic lesions of pigs experimentally inoculated with the Chilean isolate of porcine reproductive and respiratory syndrome virus. Twelve 3-week-old pigs were divided in 4 groups of 3, one of which corresponded to the negative control group sacrificed at 0 days post-inoculation (dpi, and the 3 remaining groups corresponded to the inoculated pigs sacrificed at 7, 14 and 21 dpi. For each sampling period blood was collected for complete haemograme and at the necropsy time gross lesions were registered and samples for both bone marrow smears and histopathology were taken. The results of this study revealed haematological alterations characterized by a significant reduction (P<0.05 in the haematocrit and a significant increase (P<0.05 in the total leukocyte count associated with an increase in the monocytes and baciliforms. The bone marrow did not show significant variations in the ratio of myeloid to erythroid cells (P0.05. At the same time, the gross lesions were mild and mainly characterized by the presence of conjunctivitis, periocular edema and a slight increase in the size of the lymph

  18. Effects of pork vs veal consumption on serum lipids in healthy subjects Efecto del consumo de cerdo y de ternera sobre el perfil lipídico de sujetos sanos

    Directory of Open Access Journals (Sweden)

    J. A. Rubio

    2006-02-01

    Full Text Available AIMS: To analyse the influence of lean pork (P and veal (V consumption on the lipid profile of healthy subjects within the framework of a healthy diet comprising low levels of total fat (TF, saturated fatty acids (SFA and cholesterol. DESIGN: Double-crossover, randomized and controlled trial SUBJECTS: 44 healthy individuals (22 male and 22 female, recruited voluntarily from the University Complutense of Madrid. The weight and lipid profiles of these volunteers were normal and their dietary patterns were typical for people in our area. INTERVENTIONS: The study comprised 4 phases: stabilisation phase (5 weeks, the participants followed their normal diet; second phase (6 weeks, half of the subjects, were randomised to lean pork or veal consumption, 150 g per day, for their main meal of the day; washout period (5 weeks and final phase, which was the second phase of intervention (6 weeks. During the intervention stages, only the main meal of the day was taken in the Hospital. The rest of the subjects' diets consisted of different fortnightly menus designed in accordance with the recommendations of the Spanish Society of Arteriosclerosis (SEA. RESULTS: After both stages of intervention had been completed, there was a mean reduction of 5.5% in lowdensity lipoprotein cholesterol. However, after each intervention there were no significant differences between those who had consumed P, 2.62 (0.55 mmol/L and those who had consumed V, 2.71 (0.47 mmol/L. No differences were observed in any of the other parameters between those who had consumed P and those who had consumed V. CONCLUSIONS: Lean pork and veal produces similar effects on the lipid profiles of healthy subjects. Its consumption, as part of the saturated fat and cholesterolcontrolled diet, could therefore be included in food guidelines, both for normal and therapeutic diets.OBJETIVO: Analizar la influencia del consumo de carne magra de cerdo (P y de ternera (V en el perfil lipídico de sujetos sanos

  19. La prostitución, una mirada desde sus actores

    OpenAIRE

    Luisa Fernanda Montoya Restrepo; Santiago Alberto Morales Mesa

    2015-01-01

    Este artículo surge de la investigación Aspectos sociales y familiares que conllevan al ejercicio de la prostitución a 14 jóvenes de la ciudad de Medellín, a partir de sus relatos de vida, que en su proceso de generación de información implementó entre otras estrategias, una serie de talleres con población –hombres y mujeres– en ejercicio de la prostitución y con el equipo profesional que les brinda atención psicosocial, como parte del desarrollo del diseño metodológico planteado para la gene...

  20. LA CRISIS FINANCIERA EN ESTADOS UNIDOS Y SUS CONSECUENCIAS INTERNACIONALES

    Directory of Open Access Journals (Sweden)

    Alejandro Dabat

    2009-01-01

    Full Text Available El presente artículo considera la crisis internacional basada en el sistema financiero estadounidense desde una perspectiva analítica que trata de unir tres aspectos íntimamente ligados en la generación y el desenlace de la misma, 1 La naturaleza de la crisis situada esencialmente en el sistema financiero de Estados Unidos, su relación con la especulación inmobiliaria y sus consecuencias internacionales, 2 El deterioro preexistente de la competitividad sistémica y la hegemonía de Estados Unidos desde comienzos del siglo actual, y 3 Los cambios neoeconómicos y geopolíticos mundiales de los últimos que determinan el actual reordenamiento del contexto internacional. En ese sentido, trata de unir los aspectos financieros, tecnoeconómicos y sociopolíticos de la crisis con la naturaleza de los grandes cambios mundiales que la misma implica.