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Sample records for cercosporiose cercospora coffeicola

  1. Elaboração e validação de escala diagramática para a cercosporiose do pimentão Development and validation of a diagrammatic key for Cercospora leaf spot of sweet pepper

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    Sami Jorge Michereff

    2006-09-01

    Full Text Available A cercosporiose do pimentão, causada pelo fungo Cercospora capsici, é uma importante doença que ocorre em condições tropicais. Devido à inexistência de métodos padronizados para quantificação desta doença em campo, uma escala diagramática com os níveis de 1,5; 3,5; 8,0; 16,5; 31,0 e 50% de área foliar lesionada foi elaborada e testada para a acurácia, a precisão e a reprodutibilidade das estimativas de severidade da cercosporiose do pimentão, sem e com a sua utilização. Na validação da escala diagramática, 50 folhas com diferentes níveis de severidade da doença, mensurados previamente com o programa AutoCAD®, foram avaliadas por 13 avaliadores, sem e com a utilização da escala diagramática. Foram realizadas duas avaliações com utilização da escala, com intervalo de sete dias, onde seqüências diferentes das mesmas folhas foram estimadas visualmente pelos mesmos avaliadores. A acurácia e a precisão de cada avaliador foi determinada por regressão linear simples, entre a severidade real e a estimada. Sem a escala, oito avaliadores superestimaram significativamente a severidade da doença. Com a escala, os avaliadores obtiveram melhores níveis de acurácia e precisão, embora quatro tendessem a superestimar a severidade, com os erros absolutos concentrando-se abaixo de 10%. Os avaliadores apresentaram boa repetibilidade e elevada reprodutibilidade das estimativas com a utilização da escala, o mesmo não sendo verificado sem a utilização desta. A escala diagramática proposta mostrou-se adequada para avaliação da severidade da cercosporiose do pimentão.Cercospora leaf spot of sweet pepper caused by the fungus Cercospora capsici is an important disease occurring in tropical conditions. Due to the inexistence of standard methods to quantify this disease in field conditions, a diagrammatic key including the levels 1.5, 3.5, 8.0,16.5, 31.0 and 50.0% of diseased leaf area was elaborated and tested for accuracy

  2. Potential of essential oils for the control of brown eye spot in coffee plants Potencial de óleos essenciais no controle da cercosporiose-do-cafeeiro

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    Ricardo Borges Pereira

    2011-02-01

    Full Text Available The objectives of this work were to assess the in vitro effect of essential oils extracted from cinnamon, citronella, lemon grass, India clove, tea tree, thyme, neem and eucalyptus on the conidia germination and on mycelial growth of Cercospora coffeicola, and their efficacy to control the brown eye spot in coffee seedlings (cultivars Catucaí 2SL, Catuaí IAC 62 and Mundo Novo 379/19 in a greenhouse, as well as their effects on the initial germination and infection events by scanning electron microscopy. All essential oils promoted the inhibition of conidia germination with increasing concentrations. India clove, cinnamon, neem, thyme and lemon grass oils inhibited the mycelial growth of C. coffeicola. The cinnamon and citronella oils were the most promising for brown eye spot control in all cultivars. In scanning electron microscopy, the cinnamon and citronella oils reduced germination and mycelial development of C. coffeicola in vivo, eight and 16 hours after inoculation, promoting, in some cases, the leakage of the cellular content. Essential oils of cinnamon and citronella reduced the incidence and severity of brown eye spot, in addition to presenting direct toxicity to the pathogen.Este trabalho avaliou o efeito in vitro de óleos essenciais extraídos de canela, citronela, capim-limão, cravo-da-índia, árvore-de-chá, tomilho, nim e eucalipto na germinação de conídios e no crescimento micelial de Cercospora coffeicola, a eficácia destes óleos no controle da cercosporiose-do-cafeeiro em mudas das cultivares Catucaí 2SL, Catuaí IAC 62 e Mundo Novo 379/19 em casa de vegetação; e seus efeitos sobre os eventos iniciais de germinação e infecção do patógeno in vivo por meio de microscopia eletrônica de varredura. Todos os óleos essenciais inibiram a germinação dos conídios com o aumento das concentrações. Os óleos de cravo-da-índia, canela, nim, tomilho e capim-limão inibiram o crescimento micelial de C. coffeicola. Os

  3. Intensidade da cercosporiose em mudas de cafeeiro em função de fontes e doses de silício Effect of silicon doses and sources on the intensity of the brown eye spot of coffee seedlings

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    Deila M. Santos Botelho; Edson A. Pozza; Adélia A. A. Pozza; Janice G. Carvalho; Cesar E. Botelho; Paulo E. Souza

    2005-01-01

    O objetivo deste estudo foi avaliar o efeito dos silicatos de cálcio e de sódio sobre a intensidade da cercosporiose (Cercospora coffeicola) em mudas de cafeeiro (Coffea arabica), cultivar Catuaí IAC 99, nas doses 0; 0,32; 0,64; 1,26 g de SiO2.kg-1 de substrato. Foram realizadas cinco avaliações quinzenais nas quais se quantificou o número de plantas doentes, o número de folhas lesionadas por planta, o número de lesões por folha e o número total de lesões por planta. Essas avaliações foram ut...

  4. Incidência e severidade da cercosporiose do cafeeiro em função do suprimento de potássio e cálcio em solução nutritiva Incidence and severity of the brown eye spot of coffee according to supply of potassium and calcium in nutrient solution

    OpenAIRE

    Daniel G. Júnior; Edson A. Pozza; Adélia A. A. Pozza; Paulo E. Souza; Janice G. Carvalho; Alexandre C. Balieiro

    2003-01-01

    O objetivo deste trabalho foi avaliar a incidência e a severidade da cercosporiose causada por Cercospora coffeicola em mudas de cafeeiro (Coffea arabica) cultivar Mundo Novo IAC 379-19, em função do suprimento de K e Ca em solução nutritiva. Instalou-se o experimento em DIC, com 16 tratamentos, três repetições e duas plantas por repetição. Utilizou-se esquema fatorial 4 x 4, com quatro doses de K (1, 3, 5 e 7 mmol/l) e quatro níveis de Ca (2, 4, 6 e 8 mmol/l). As mudas foram inoculadas seman...

  5. Incidência e severidade da cercosporiose do cafeeiro em função do suprimento de potássio e cálcio em solução nutritiva Incidence and severity of the brown eye spot of coffee according to supply of potassium and calcium in nutrient solution

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    Daniel G. Júnior

    2003-06-01

    Full Text Available O objetivo deste trabalho foi avaliar a incidência e a severidade da cercosporiose causada por Cercospora coffeicola em mudas de cafeeiro (Coffea arabica cultivar Mundo Novo IAC 379-19, em função do suprimento de K e Ca em solução nutritiva. Instalou-se o experimento em DIC, com 16 tratamentos, três repetições e duas plantas por repetição. Utilizou-se esquema fatorial 4 x 4, com quatro doses de K (1, 3, 5 e 7 mmol/l e quatro níveis de Ca (2, 4, 6 e 8 mmol/l. As mudas foram inoculadas semanalmente, durante 12 semanas, com suspensão de esporos de C. coffeicola na concentração de 15.000 conídios/ml. Foram realizadas sete avaliações quinzenais para a obtenção da área foliar total, número total de folhas e número total de lesões de cada planta. Após as avaliações determinou-se a porcentagem de área foliar lesionada e o peso da matéria seca total. A interação K x Ca influenciou a área abaixo da curva de progresso do total de lesões, do número total de folhas e a porcentagem de área foliar lesionada. As doses de K, isoladamente, influenciaram a matéria seca total, a área foliar total e a área abaixo da curva de progresso da incidência. As doses de Ca, isoladamente, influenciaram apenas a área foliar total e a área abaixo da curva de progresso da incidência.An experiment was carried out at UFLA to evaluate the incidence and severity of the brown eye spot caused by Cercospora coffeicola in coffee (Coffea arabica seedlings, in relation to different dosages of K and Ca in nutrient solution. The experimental design was completely randomized with 16 treatments, three replicates and two plants per replicate. Treatments were displayed in a 4 x 4 factorial scheme, with four levels of K (1, 3, 5 and 7 mmol/l and four levels of Ca (2, 4, 6 and 8 mmol/l. The seedlings were inoculated, every week, for three months, with a conidial suspension of C. coffeicola of 15,000 conidia/ml. Total leaf area, total number of leaves and

  6. ACTION OF SILICATO OF CALCIUM AND MAGNESIUM IN THE REDUCTION Cercospora

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    Franciane Diniz Cogo

    2011-07-01

    Full Text Available The aim of this study was to evaluate the response of coffee plants (Coffea arabica Catuaí IAC-44 with different doses of calcium silicate and magnesium in relation to incidence of Cercospora spot coffeicola and plant growth. The experiment was conducted in nursery seedling production of coffee from the Federal Institute of Southern Minas Gerais, Campus Machado, MG randomized blocks with five replications and the treatment used a source of silicon, calcium silicate and magnesium in doses - 0 (control, 75, 150, 225 300 g/m2. The application of silicon in the soil causes the reduction to Cercospora coffeicola. The application of calcium silicate and magnesium affect the growth characteristics and was effective in inducing gray leaf spot, via ground coffee seedlings in nurseries.

  7. ACTION OF SILICATO OF CALCIUM AND MAGNESIUM IN THE REDUCTION Cercospora

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    Franciane Diniz Cogo; Gabriel Sergio Graciano; Katia Alves Campos

    2011-01-01

    The aim of this study was to evaluate the response of coffee plants (Coffea arabica) Catuaí IAC-44 with different doses of calcium silicate and magnesium in relation to incidence of Cercospora spot coffeicola and plant growth. The experiment was conducted in nursery seedling production of coffee from the Federal Institute of Southern Minas Gerais, Campus Machado, MG randomized blocks with five replications and the treatment used a source of silicon, calcium silicate and magnesium in doses - 0...

  8. Intensidade da cercosporiose em mudas de cafeeiro em função de fontes e doses de silício Effect of silicon doses and sources on the intensity of the brown eye spot of coffee seedlings

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    Deila M. Santos Botelho

    2005-12-01

    Full Text Available O objetivo deste estudo foi avaliar o efeito dos silicatos de cálcio e de sódio sobre a intensidade da cercosporiose (Cercospora coffeicola em mudas de cafeeiro (Coffea arabica, cultivar Catuaí IAC 99, nas doses 0; 0,32; 0,64; 1,26 g de SiO2.kg-1 de substrato. Foram realizadas cinco avaliações quinzenais nas quais se quantificou o número de plantas doentes, o número de folhas lesionadas por planta, o número de lesões por folha e o número total de lesões por planta. Essas avaliações foram utilizadas para construir a área abaixo da curva de progresso da doença. Ao término das avaliações, foram determinados os teores de macro, micronutrientes, silício e lignina na parte aérea das mudas de cafeeiro. A menor área abaixo da curva de progresso do total de lesões foi obtida com a dose de 0,84 g.kg-1 de silicato de sódio. Observou-se decréscimo linear para área abaixo da curva de progresso do número de plantas doentes e aumento na concentração de lignina nas folhas até a dose de 0,52 g.kg-1 de silicato de sódio, enquanto no caule houve acúmulo de SiO2 até 0,53 g.kg.-1.The objective of this work was to evaluate the effect of calcium and sodium silicate on the intensity of brown eye spot (Cercospora coffeicola in coffee (Coffea arabica seedlings (Catuaí IAC 99 by using doses (0; 0.32; 0.63 and 1.26 g of SiO2.kg-1 of substrate. Five evaluations were done by counting the number of diseased plants, number of leaves with lesions, number of lesions per leaf and total number of lesions per plant. The concentration of macro, micronutrients, silicon and lignin in the leaves was determined at the end of the experiment. The lowest area under the disease progress curve of the total number of lesions was observed with 0.84 g.kg-1 of sodium silicate. Diseased plants were found to have a linear decrease of area under the disease progress curve and an increase in the concentration of lignin up to the dose of 0.52 g.kg-1 of sodium silicate

  9. Progresso temporal da cercosporiose da beterraba em diferentes genótipos e épocas de semeadura na primavera

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    Leandro Luiz Marcuzzo

    2015-09-01

    Full Text Available A cultura da beterraba tem se intensificado devido ao consumo in natura e também pelo processamento industrial de conservas. Na região do Alto Vale do Itajaí, SC, grande parte da sua produção vem de agricultores familiares que a usam em sucessão a cultura da cebola, com semeadura na primavera/verão. Entretanto, nesta época do ano a cercosporiose (Cercospora beticola tem comprometido a produtividade. O objetivo do trabalho foi avaliar o progresso desta doença nos genótipos: All Green, Stays Green, Early Wonder, Cabernet, Boro, Modana e Itapuã e avaliar a severidade em duas épocas de semeadura, na primavera. Os dois ensaios foram conduzidos na Epagri/Estação Experimental de Ituporanga, implantados com 30 dias de diferença entre as semeaduras. O delineamento experimental foi de blocos casualizados com quatro repetições. Para avaliação da severidade da doença foi utilizada uma escala diagramática em dez plantas ao acaso previamente demarcadas. Os dados de severidade foram submetidos à análise de modelos mistos, area abaixo da curva de progresso de doença, taxa de infecção e severidade final. Não houve diferenças no progresso da doença entre os genótipos avaliados nas duas épocas de semeadura de primavera nas condições do Alto Vale do Itajaí, SC.

  10. Aerobiologia de Cercospora kikuchii Aerobiology of Cercospora kikuchii

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    Ângela Sathiko Kudo

    2011-10-01

    Full Text Available Neste estudo, foram quantificados os conídios de Cercospora kikuchii [crestamento foliar em soja (Glycine max] presentes no ar, sua relação com a intensidade da doença e com os fatores ambientais [temperatura, umidade relativa do ar (UR, precipitação e molhamento foliar] no período de novembro a março de 2006/07 e 2007/08 em Brasília, DF. Para tanto, foi instalada uma armadilha volumétrica capta-esporos 'Burkard' de sete dias em campo experimental com soja. Houve maior captura de conídios no período diurno, com mais de 60% dos esporos coletados entre 8h e 15h. Os dados climáticos variaram de acordo com a safra, mas, de modo geral, em ambos os experimentos, o período de maior captura de esporos ocorreu quando houve redução da precipitação e do molhamento foliar. A UR acima de 80% e temperatura entre 20 e 24°C foram mais favoráveis para a captura de conídios e a intensidade do crestamento foliar. Nos dois anos de avaliação, a maior quantidade de esporo capturado ocorreu quando a soja se apresentava no estádio R6-R7, assim como a intensidade da doença foi maior após o início do estágio reprodutivo da cultura. Apesar da quantidade de conídio capturado em 2006/07 ter sido maior que em 2007/08, o padrão da flutuação aérea de conídios foi semelhante.The objective of this study was to evaluate the relation of airborne released conidia of Cercospora kikuchii [cercospora leaf blight of soybean (Glycine max] with the disease intensity and some environmental factors [temperature, relative humidity (RH, precipitation and leaf wetness period under experimental field conditions in Brasília, DF, Brazil. The evaluation was from November to March of 2006/07 and 2007/08. Data for the amount of airborne conidia were collected using a Burkard's seven-day volumetric spore trap set in a field with soybean. Most of the conidia were collected during the day, with over 60% of the spores collected between 8h and 15h. Climatic data varied

  11. Effect of crop density of two genotypes of maize in the severity of gray leaf spot and yield in the second season cropInfluência da densidade de cultivo de dois genótipos de milho na severidade da mancha de cercospora e no rendimento de grãos na safrinha

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    Luis Gustavo Amorim Pessoa

    2012-08-01

    Full Text Available Among the diseases that affect the corn crop and can reduce the yield, there is cercospora gray leaf, caused by Cercospora spp. This disease is very important in the second season crop or ‘safrinha’. The objective of this study was to evaluate the severity of cercospora leaf spot in transgenic corn hybrids Agroceres Yieldgard AG 9010 YG and Advance NK Agrisure TL (Bt11, using two plant densities: 78,000 and 100,000 plants per hectare. The results showed that the higher density of plants increased the severity of the disease and provided a lower yield. There was no significant interaction between hybrids and severity of the disease. Entre as doenças que incidem na cultura do milho e podem reduzir seu rendimento, há a mancha de cercospora, causada por Cercospora spp., especialmente importante em milho de segunda época, ou ‘safrinha’. O objetivo deste trabalho foi avaliar a severidade da cercosporiose em nos híbridos transgênicos de milho Agroceres Yieldgard AG 9010 YG e Advance NK Agrisure TL (Bt11, em duas densidades de cultivo: 78.000 e 100.000 plantas por hectare. Os resultados permitiram constatar que a menor densidade de plantas favoreceu o incremento da severidade da doença e proporcionou menor rendimento de grãos. Não houve interação significativa entre os híbridos e a severidade da doença.

  12. Efeito do meio de cultura e do regime de luz na esporulação de Cercospora zeae-maydis Effect of culture media and light exposure on the sporulation of Cercospora zeae-maydis

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    Kátia Regiane Brunelli

    2006-03-01

    Full Text Available Algumas espécies fúngicas não esporulam satisfatoriamente em meio de cultura, a exemplo de Cercospora zeae-maydis, agente causal da cercosporiose do milho. A esporulação deste patógeno foi avaliada em sete meios de cultura agarizados (V8, suco de tomate temperado, água de coco, aveia, BDA, extrato de folha de milho e extrato de folha de milho + CaCO3 sob dois regimes luminosos (fotoperíodo de 12 horas e seqüencial - 6 dias claro/3 dias escuro. O ensaio foi conduzido em esquema fatorial 7 x 2, com os tratamentos dispostos em delineamento inteiramente casualizado com cinco repetições. A parcela experimental compreendeu uma placa de petri contendo 20 mL de meio de cultura sobre o qual foram colocados 200 mL de uma suspensão de 8 x 10(4 esporos/mL. As culturas foram posteriormente incubadas a 27ºC durante nove dias. Os meios V8 e suco de tomate temperado (STT sob regime de fotoperíodo 12h/12h, foram aqueles que apresentaram melhor indução de esporulação, resultando na produção de 22,4 x 10(4 conídios/ mL e 28,62 x 10(4 conídios/mL, respectivamente.Some fungal species, like Cercospora zeae-maydis, causal agent of maize gray leaf spot, do not satisfactorily produce spores in artificial media. The conidial production of C. zeae-maydis was evaluated on seven culture media (V8, tomato juice, coconut water, oat, PDA, maize leaf extract and maize leaf extract plus CaCO3 under two light exposure regimens (12-hours photoperiod or six days under continuous light followed by three days of continuous darkness. The experiment was arranged as a 7 x 2 factorial design in a completely randomized design with five replicates. A single petri dish containing 20 mL of culture media inoculated with 200 mL of conidial suspension (8 x 10(4 conidia/mL comprised the experimental unit. Plates were incubated at 27ºC for nine days. The highest conidial production was obtained on V8 and tomato juice media under 12-hours photoperiod, resulting in the

  13. Controle genético da resistência da soja à raça 4 de Cercospora sojina

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    Martins Filho Sebastião

    2002-01-01

    Full Text Available O objetivo deste trabalho foi estudar o controle genético da resistência à Cercospora sojina, em populações segregantes derivadas do cruzamento entre as cultivares de soja Paraná e Bossier. Foram avaliadas nos genitores e nas gerações F1, F2, RC1 e RC2 seis características das plantas associadas com a doença: nota para infecção (NT; número de lesões por folíolo (NLF; diâmetro médio de lesão (DML; porcentagem de área foliar lesionada (PAFL; número de lesões por cm² (NLC e índice de doença (ID. A resistência da soja à cercosporiose comportou-se como um caráter quantitativo, e o efeito gênico aditivo o mais importante. As influências sobre NT foram as seguintes: efeito aditivo (62,05%, efeito da dominância (7,68% e as interações epistáticas aditiva x aditiva, aditiva x dominante e dominante x dominante (7,32%. O modelo aditivodominante foi suficiente para explicar as variações somente nos caracteres PAFL e NLC. A influência dos efeitos das interações epistáticas variaram de 2,22% no caráter PAFL e em até 30,78% no caráter DML. O modelo genético aditivo-dominante é satisfatório para explicar o comportamento da média das gerações em relação aos caracteres PAFL e NLC. Entretanto, quanto a NT, NLF, DML e ID, o modelo aditivo-dominante-epistático é o mais adequado.

  14. Controle químico da Cercosporiose, Mancha-Branca e dos Grãos Ardidos em milho

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    André Humberto Brito

    2013-10-01

    Full Text Available Este trabalho foi realizado com o objetivo de avaliar os efeitos da aplicação de fungicida, no controle da Cercosporiose e da Mancha-Branca do milho, e suas consequências na produtividade de grãos e na incidência de grãos ardidos, além de estabelecer a relação existente entre a produtividade de grãos e a severidade dessas doenças. Dois experimentos distintos (com Azoxystrobina + Cyproconazole e tratamento controle foram conduzidos em três locais, no ano agrícola de 2007/2008. Utilizaram-se 12 híbridos comerciais de milho, que foram avaliados em delineamento de blocos casualizados, com três repetições. Foram realizadas cinco avaliações da severidade das doenças Cercosporiose e Mancha-Branca, por meio de escala de notas, variando de 1 (altamente resistente a 9 (altamente susceptível. Estimou-se a área abaixo da curva de progresso da doença (AACPD. A aplicação de fungicida é eficiente no controle de doenças foliares e na redução da incidência de grãos ardidos, além de proporcionar 12% de aumento na produtividade de grãos, em relação à do tratamento controle. As doenças foliares Cercosporiose e Mancha-Branca reduzem a produtividade de grãos de milho e essa redução é maior quando as doenças ocorrem mais precocemente. A Cercosporiose provoca maior redução na produtividade de grãos, quando comparada com a Mancha-Branca.

  15. Novel species of Cercospora and Pseudocercospora (Capnodiales, Mycosphaerellaceae) from Australia.

    Science.gov (United States)

    Shivas, Roger Graham; Marney, Thomas Stephen; Tan, Yu Pei; McTaggart, Alistair Ross

    2015-05-01

    Novel species of Cercospora and Pseudocercospora are described from Australian native plant species. These taxa are Cercospora ischaemi sp. nov. on Ischaemum australe (Poaceae); Pseudocercospora airliensis sp. nov. on Polyalthia nitidissima (Annonaceae); Pseudocercospora proiphydis sp. nov. on Proiphys amboinensis (Amaryllidaceae); and Pseudocercospora jagerae sp. nov. on Jagera pseudorhus var. pseudorhus (Sapindaceae). These species were characterised by morphology and an analysis of partial nucleotide sequence data for the three gene loci, ITS, LSU and EF-1α. Recent divergence of closely related Australian species of Pseudocercospora on native plants is proposed. PMID:25937064

  16. Aggressiveness between genetic groups I and II of isolates of Cercospora zeae-maydis Agressividade entre isolados dos grupos genéticos I e II de Cercospora zeae-maydis

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    Sandra Marisa Mathioni

    2006-12-01

    Full Text Available For many years, the gray leaf spot disease (GLS caused by the fungus Cercospora zeae-maydis Tehon & Daniels, was not considered an important pathogen of maize (Zea mays, L. in Brazil. However, the recent adoption of agronomical practices such as no-tillage and cultivation under central pivot irrigation systems increased the incidence and severity to the extent that GLS is now one of the most important diseases of maize. Isolates of C. zeae-maydis can be distinguished by two genetic groups (I and II based on AFLP markers and on polymorphisms of the ITS and 5.8S rDNA regions. Until now, however, the biological implications of this distinction remain unclear. This study investigated whether isolates from the two genetic groups differ in aggressiveness towards maize. For this, symptoms of a susceptible hybrid were evaluated under greenhouse conditions with 9 and 11 isolates of C. zeae-maydis from groups I and II, respectively. Plants in the V3 growth stage were inoculated by placing sorghum seeds colonized with the pathogen in the leaf whorl and symptoms were evaluated with a visual rating scale 30 days later. On average, isolates of genetic group II were more aggressive than those of group I, with mean disease scores of 3.1 and 2.3, respectively. Differences were also observed between experiments, which suggested that group I and II might also differ in their fitness under different environments. This is the first report on differences in aggressiveness between the two genetic groups of C. zeae-maydis.Durante muitos anos, a cercosporiose, causada pelo fungo Cercospora zeae-maydis Tehon & Daniels, não foi considerada importante para a cultura do milho (Zea mays, L. no Brasil. Entretanto, a recente utilização de práticas culturais como o plantio direto e o cultivo sob pivôs centrais favoreceram o aumento de sua severidade e incidência, de forma que a doença é hoje considerada uma das mais importantes da cultura. Isolados de C. zeae

  17. A rapid diagnostic tool for detecting benzimidazole resistance in Cercospora beticola, the causal agent of Cercospora leaf spot in sugarbeet

    Science.gov (United States)

    Resistance in Cercospora beticola to benzimidazole fungicides was selected in many sugar beet regions of the world due to extensive use of this fungicide class. Practical resistance to benzimidazole in populations of C. beticola was first reported in Greece in 1973. Resistance has subsequently been ...

  18. Multilocus Sequence Analysis of Cercospora spp. from Different Host Plant Families

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    Floreta Fiska Yuliarni; Wellyzar Sjamsuridzal; Iman Hidayat

    2014-01-01

    Identification of the genus Cercospora is still complicated due to the host preferences often being used as the main criteria to propose a new name. We determined the relationship between host plants and multilocus sequence variations (ITS rDNA including 5.8S rDNA, elongation factor 1-α, and calmodulin) in Cercospora spp. to investigate the host specificity. We used 53 strains of Cercospora spp. infecting 12 plant families for phylogenetic analysis. The sequences of 23 strains of ...

  19. Fungicide sensitivity testing of Cercospora beticola from sugarbeet in the Great Lakes Region

    Science.gov (United States)

    Cercospora leaf spot (CLS), caused by Cercospora beticola (Sacc.), is the most serious foliar disease of sugarbeet (Beta vulgaris L.) worldwide. CLS is problematic in most sugarbeet growing regions and can cause significant economic losses due to decreases in both yield and sugar purity in the harve...

  20. Assessment of strobilurin resistance in Cercospora beticola in on sugar beet in Michigan and Nebraska, USA

    Science.gov (United States)

    Cercospora leaf spot (CLS) caused by Cercospora beticola Sacc. is the most important foliar disease of sugar beet (Beta vulgaris) worldwide. CLS is controlled mainly with fungicides, including strobilurins (FRAC group 11). Resistance to strobilurins in C. beticola was first identified in 2011 from s...

  1. First report of QoI insensitive Cercospora beticola on sugarbeet in Ontario, Canada

    Science.gov (United States)

    Cercospora beticola causes Cercospora leaf spot (CLS) of sugarbeet (Beta vulgaris) and is the most destructive foliar disease of sugarbeet worldwide. The QoI fungicide pyraclostrobin has been an important management tool for CLS in Canada since 2003. Beginning in 2010 some growers reported unsatisf...

  2. Resistance to stem canker, frogeye leaf spot and powdery mildew of soybean lines lacking lipoxigenases in the seeds Resistência ao cancro-da-haste, à cercosporiose e ao oídio de linhagens de soja sem lipoxigenases nas sementes

    Directory of Open Access Journals (Sweden)

    Carlos Alberto Osório Martins

    2002-12-01

    ãos. A presença de gosto de feijão cru tem sido limitante para o consumo de derivados de soja pelos povos ocidentais. Esse sabor característico é proporcionado pelas enzimas lipoxigenases (Lox1, Lox2 e Lox3. A eliminação dessas enzimas, pela manipulação genética dos alelos que as codificam, é a maneira mais adequada de contornar os problemas associados ao sabor desagradável. Visando elucidar a participação das lipoxigenases, no processo de resistência da soja a patógenos, variedades normais de soja (FT-Cristalina RCH, Doko RC e IAC-12 e suas respectivas linhagens obtidas por retrocruzamentos, sem as três lipoxigenases nas sementes (triplo-nulas - TN e com as três lipoxigenases (triplo-positivas - TP, foram testadas quanto às suas resistências ao cancro-da-haste (Diaporthe phaseolorum f.sp. meridionalis, à cercosporiose (Cercospora sojina Hara e ao oídio (Microsphaera diffusa Cke. & Pk.. Todos os materiais genéticos foram resistentes ao cancro-da-haste. Com relação à cercosporiose, FT-Cristalina RCH e Doko-RC e suas respectivas linhagens com ou sem lipoxigenases mostraram-se resistentes, enquanto IAC-12 e suas linhagens derivadas mostraram índices de doença mais elevados, sendo que as linhagens IAC-12 TP e TN foram mais suscetíveis, indicando perda de gene de resistência nos retrocruzamentos. Não houve relação entre retirada dos genes que codificam lipoxigenases nas sementes com a resistência à cercosporiose. No caso do oídio, as linhagens TP ou TN apresentaram-se similares ou pouco mais resistentes que seus respectivos progenitores recorrentes, os quais se mostraram suscetíveis na seguinte ordem: IAC-12, menos suscetível, Doko-RC, intermediário e FT-Cristalina RCH, mais suscetível.

  3. Cercospora apii causing leaf spots on two Brazilian toxic weeds: solanum glaucophyllum and Xanthium strumarium

    OpenAIRE

    Fabiano Branco Rocha; Olinto Liparini Pereira; Robert Weingart Barreto

    2007-01-01

    Samples of two toxic weeds, Solanum glaucophyllum and Xanthium strumarium, bearing leaf spots symptoms were found. Cercospora apii was found associated to these leaf spots in both plants. These are the first reports of this fungus on these hosts in Brazil and its first report on S. glaucophyllum worldwide.Foram encontrados plantas das espécies Solanum glaucophyllum e Xanthium strumaruium apresentando sintomas de manchas foliares. Cercospora apii foi encontrado associado lesões foliares em amb...

  4. Morphological characteristic of Cercospora beticola (Sacc.) from infected sugar beet leaves and determination of its growth medium.

    OpenAIRE

    Göktürk, T.; Döken, M.T.

    2008-01-01

    This study was carried out at the sugar beet field in Oltu (Erzurum) about 30-50% of the area was infected by Cercospora beticola  (Sacc.). Isolates were collected from the infected sugar beet leaves. The collected isolates were transferred into the plates, which contain PDA (Potato Decroze Agar) and SBLA (Sugar Beet Leaves Extract Agar). After the germination, Cercospora beticola (Sacc.) showed differences between conidia and conidiophore sizes. Cercospora beticola sporulated in SBLEA...

  5. RNA-sequencing of Cercospora beticola DMI-sensitive and -resistant isolates after treatment with tetraconazole identifies common and contrasting pathway induction

    NARCIS (Netherlands)

    Bolton, Melvin D.; Ebert, Malaika K.; Faino, Luigi; Rivera-Varas, Viviana; Jonge, de Ronnie; Peer, Van de Yves; Thomma, Bart P.H.J.; Secor, Gary A.

    2016-01-01

    Cercospora beticola causes Cercospora leaf spot of sugar beet. Cercospora leaf spot management measures often include application of the sterol demethylation inhibitor (DMI) class of fungicides. The reliance on DMIs and the consequent selection pressures imposed by their widespread use has led to

  6. Progresso da ferrugem e da cercosporiose do cafeeiro consorciado com grevílea, com ingazeiro e a pleno sol em Lavras - MG Progress of rust and coffee plant cercosporiose mixed with grevílea, with ingazeiro and in the full sunshine in Lavras - MG

    Directory of Open Access Journals (Sweden)

    Bruno Grandi Salgado

    2007-08-01

    Full Text Available Com o presente trabalho, objetivou-se avaliar a incidência da ferrugem e da cercosporiose do cafeeiro em diferentes sistemas de cultivo, agroflorestais e a pleno sol, através da curva de progresso dessas doenças. Utilizou-se o delineamento inteiramente casualizado, com três tratamentos e sete repetições. Os tratamentos foram compostos por cafeeiros consorciados com ingazeiro, cafeeiros consorciados com grevílea e cafeeiros cultivados convencionalmente a pleno sol. As avaliações foram realizadas mensalmente no período de abril de 2001 a março de 2003. Realizou-se o cálculo da área abaixo da curva de progresso da doença (AACPD, a qual foi submetida à análise de variância. Para a incidência da ferrugem do cafeeiro o consórcio cafeeiro x ingazeiro mostrou-se com maiores índices da doença, sendo que os tratamentos consórcio cafeeiro x grevílea e cafeeiro a pleno sol não diferiram entre si e apresentaram menores incidências da doença. Diminuição na luz solar direta e maior umidade podem ter favorecido a ferrugem no sistema cafeeiro x ingazeiro. Para a incidência de cercosporiose, os cafeeiros a pleno sol obtiveram maiores valores de incidência da doença, seguidos por cafeeiros x grevílea, e menores taxas da doença foram observadas no consórcio cafeeiros x ingazeiro. A incidência de radiação solar direta pode ter favorecido a maior incidência de cercosporiose nos cafeeiros a pleno sol.The present work aimed to evaluate the incidence of rust and coffee plant cercosporiose in different systems of agroforest cultivation and in the full sunshine through the progress curve of those diseases. The completely randomized design with three treatments and seven replicates was utilized. The treatments were made up of coffee plants mixed with ingazeiro, coffee plants mixed with grevílea and coffee plants cultivated conventionally in the full sunshine. The evaluations were performed monthly during the period of April 2001 to

  7. Cercospora apii causing leaf spots on two Brazilian toxic weeds: solanum glaucophyllum and Xanthium strumarium

    Directory of Open Access Journals (Sweden)

    Fabiano Branco Rocha

    2007-03-01

    Full Text Available Samples of two toxic weeds, Solanum glaucophyllum and Xanthium strumarium, bearing leaf spots symptoms were found. Cercospora apii was found associated to these leaf spots in both plants. These are the first reports of this fungus on these hosts in Brazil and its first report on S. glaucophyllum worldwide.Foram encontrados plantas das espécies Solanum glaucophyllum e Xanthium strumaruium apresentando sintomas de manchas foliares. Cercospora apii foi encontrado associado lesões foliares em ambas as plantas. Estes são os primeiros relatos desse fungo nestes hospedeiros no Brasil e o primeiro relato de sua ocorrência em S. glaucophyllum no mundo.

  8. Development of polymorphic microsatellite and single nucleotide polymorphism markers for Cercospora beticola (Mycosphaerellaceae)

    NARCIS (Netherlands)

    Groenewald, M.; Groenewald, J.Z.; Linde, C.C.; Crous, P.W.

    2007-01-01

    The plant pathogenic fungus, Cercospora beticola, causes the most important foliage disease of sugar beet. A previous study has shown that isolates of opposite mating types are present in equal proportions in natural populations; therefore, the aim of this study was to develop highly reproducible po

  9. Draft Genome Sequence of Cercospora arachidicola, Causal Agent of Early Leaf Spot in Peanuts

    Science.gov (United States)

    Cercospora arachidicola, an economically important pathogen of peanut, is the cause of early leaf spot disease. Despite its significance, insufficient genetic information is available for utilization. Understanding the genetic diversity of this pathogen is crucial for peanut breeding programs to d...

  10. The disease prevalence and severity of Cercospora leaf spot in sugar beet cultivations in Kayseri

    Directory of Open Access Journals (Sweden)

    Hacer Handan ALTINOK

    2012-12-01

    Full Text Available Cercospora leaf spot disease (Cercospora beticola Sacc., is one of the most economically important fungal diseases in sugar beet growing. Under appropriate climatic conditions, the disease can reach epidemic levels. Although some fungicides exist for disease control, resistance development by pathogen against fungicides is creating difficulties. Besides, use of resistant varieties which is considered as the most efficient and environment-friendly method is adversely affected by pathogen’s ability to exhibit high genetic variations and varying resistance levels against different races of pathogen restricts the success of resistance breeding studies. In order to reveal status of this disease in Kayseri province, surveys were conducted in 2010 and 2011 in sugar beet growing areas and disease prevalence and severity were determined. Approximately, 1500 da area in 90 fields were examined and about 700 da of this area found as infected with Cercospora leaf spot disease in both years of the survey. Highest disease prevalence and severity were found as 80 % and 45 %, respectively, in Sarıoğlan district, which is followed by central district, Develi and Bünyan. Among surveyed districts, lowest prevalence and severity were detected as approx. 65 % and 35 %, respectively, in Yeşilhisar.

  11. First Record of Cercospora Leaf Spot Disease on Okra Plants and its Control in Egypt

    Directory of Open Access Journals (Sweden)

    Eman S.H. Farrag

    2011-01-01

    Full Text Available During June to September 2008, okra plants (Hibiscus esculentus L. in Kafr El-Sheikh Governorate exhibited typical symptoms of Cercospora Leaf Spot (CLS at different locations. Symptoms of infected okra leaves firstly started as light brown spots then turned to purple and varying in size. The spots spread to cover large areas of infected leaves. In case of severe infection, spots joined together and formed patches. Later, leaves were dry and remained intact with stem of plant. Samples of diseased leaves were collected to isolate the causal organisms. Isolated fungi were purified using single spore culture technique. Developed fungus was identified as Cercospora sp. Fresen based on cultural and morphological characteristics after light microscope examination. Also, Alternaria alternate and Aspergillus niger were isolated as associated fungi. Pathoginicity test confirmed efficiency of Cercospora sp. to induce typical symptoms on okra plants compared with other fungi. Foliar application using different concentrations of Topsin M-70WP and lemongrass oil was significantly reduced disease incidence compared with control. According to the available literature, this is the first record of CLS on okra in Egypt under natural infection in the field.

  12. Mating type idiomorphs distribution and their correlation to benzimidazole-resistance in Cercospora beticola from the Central High Plains Region, USA

    Science.gov (United States)

    Cercospora leaf spot (CLS) of sugarbeet is caused by Cercospora beticola Sacc., and is one of the most destructive foliar diseases worldwide. Fungicides used for disease suppression include benzimidazoles. Resistance to benzimidazoles can develop quickly, and was first reported in C. beticola in 197...

  13. Parâmetros genéticos da resistência da soja a Cercospora sojina Genetic parameters of soybean resistance to Cercospora sojina

    OpenAIRE

    Geraldo de Amaral Gravina; Sebastião Martins Filho; Carlos Sigueyuki Sediyama; Cosme Damião Cruz

    2004-01-01

    A herança da resistência da soja a Cercospora sojina Hara foi avaliada por meio de parâmetros genéticos, estimados pela análise de médias e de variâncias de um índice multivariado. Foram utilizados os cruzamentos de duas cultivares resistentes, Paraná (P) e Uberaba (U), com uma suscetível, Bossier (B). Foram avaliados cinco caracteres associados à doença, nos genitores e nas gerações F1, F2, RCR e RCS de cada cruzamento: nota do grau de infecção avaliado visualmente; diâmetro médio da lesão; ...

  14. Improvement of Lesion Phenotyping in Cercospora beticola-Sugar Beet Interaction by Hyperspectral Imaging.

    Science.gov (United States)

    Leucker, Marlene; Mahlein, Anne-Katrin; Steiner, Ulrike; Oerke, Erich-Christian

    2016-02-01

    Cercospora leaf spot (CLS) caused by Cercospora beticola is the most destructive leaf disease of sugar beet and may cause high losses in yield and quality. Breeding and cultivation of disease-resistant varieties is an important strategy to control this economically relevant plant disease. Reliable and robust resistance parameters are required to promote breeding progress. CLS lesions on five different sugar beet genotypes incubated under controlled conditions were analyzed for phenotypic differences related to field resistance to C. beticola. Lesions of CLS were rated by classical quantitative and qualitative methods in combination with noninvasive hyperspectral imaging. Calculating the ratio of lesion center to lesion margin, four CLS phenotypes were identified that vary in size and spatial composition. Lesions could be differentiated into subareas based on their spectral characteristics in the range of 400 to 900 nm. Sugar beet genotypes with lower disease severity typically had lesions with smaller centers compared with highly susceptible genotypes. Accordingly, the number of conidia per diseased leaf area on resistant plants was lower. The assessment of lesion phenotypes by hyperspectral imaging with regard to sporulation may be an appropriate method to identify subtle differences in disease resistance. The spectral and spatial analysis of the lesions has the potential to improve the screening process in breeding for CLS resistance. PMID:26506458

  15. Magnetic resonance imaging of sugar beet taproots in soil reveals growth reduction and morphological changes during foliar Cercospora beticola infestation.

    Science.gov (United States)

    Schmittgen, Simone; Metzner, Ralf; Van Dusschoten, Dagmar; Jansen, Marcus; Fiorani, Fabio; Jahnke, Siegfried; Rascher, Uwe; Schurr, Ulrich

    2015-09-01

    Cercospora leaf spot (CLS) infection can cause severe yield loss in sugar beet. Introduction of Cercospora-resistant varieties in breeding programmes is important for plant protection to reduce both fungicide applications and the risk of the development of fungal resistance. However, in vivo monitoring of the sugar-containing taproots at early stages of foliar symptoms and the characterization of the temporal development of disease progression has proven difficult. Non-invasive magnetic resonance imaging (MRI) measurements were conducted to quantify taproot development of genotypes with high (HS) and low (LS) levels of susceptibility after foliar Cercospora inoculation. Fourteen days post-inoculation (dpi) the ratio of infected leaf area was still low (~7%) in both the HS and LS genotypes. However, during this period, the volumetric growth of the taproot had already started to decrease. Additionally, inoculated plants showed a reduction of the increase in width of inner cambial rings while the width of outer rings increased slightly compared with non-inoculated plants. This response partly compensated for the reduced development of inner rings that had a vascular connection with Cercospora-inoculated leaves. Hence, alterations in taproot anatomical features such as volume and cambial ring development can be non-invasively detected already at 14 dpi, providing information on the early impact of the infection on whole-plant performance. All these findings show that MRI is a suitable tool to identify promising candidate parent lines with improved resistance to Cercospora, for example with comparatively lower taproot growth reduction at early stages of canopy infection, for future introduction into breeing programmes. PMID:25873673

  16. PENGENDALIAN PENYAKIT PATIK (Cercospora nicotianae PADA TEMBAKAU NA OOGST SECARA IN-VIVO DENGAN EKSTRAK DAUN GULMA KIPAHIT (Tithonia diversifolia

    Directory of Open Access Journals (Sweden)

    Aditya Reza Apriyadi

    2013-11-01

    Full Text Available [ENGLISH] Frog eyes diseases or leaf spot caused by Cercospora nicotinae that can reduce the quality of tobacco leaves, especially when used as a cigar deckblad. Affected leaves was easyly torn and the syhmptoms developed rapidly when processed in the storage. Therefore the alternative control of plant is by mexico sun flower leaf extracts. Mexico sunflower belonging to the broadleaf weeds. It suggested contains flavonoids, tannins, terpenoids, and saponins. The results showed mexico sunflower leaf extract concentration 50 g / L was effective to control this diseases, when compared mexico sunflower leaf extract concentration 25 g / L. However, there was no different between plant sprayed with mexico sun flower extract at concentration of 50 g/L and 75 g/L. Keywords: Mexico Sunflower leaf extract; Cercospora nicotianae; Tobacco. [INDONESIAN] Penyakit patik atau bercak daun Cercospora yang disebabkan oleh jamur Cercospora nicotinae dapat mengurangi mutu daun tembakau, terutama apabila digunakan sebagai daun pembalut cerutu. Biasanya penyakit ini dikendalikan dengan pestisida kimia tetapi karena ada batasan residu kimia maksimum sebesar 2,0 ppm, maka dicari alternatif pengendaliannya. Kipahit merupakan gulma berdaun lebar yang mempunyai potensi sebagai anti jamur patogen tanaman, karena mengandung senyawa flavonoid, tannin, terpenoid, dan saponin. Dengan penyemprotan empat kali dalam interval 15 hari, diketahui ekatrak daun kipahit dengan konsentrasi 50 g/l dan 75 g/L dapat menurunkan tingkat keparahan penyakit 1% sejak 60 hst sampai 70 hst. Akan tetapi, nilai insiden penyakit diketahui 100% pada semua perlakuan ekstrak daun kipahit. Ekstrak daun kipahit dengan konsentrasi 50 g/L sudah efektif mengendalikan penyakit patik jika dibandingkan dengan ekstrak daun kipahit dengan konsentrasi 25 g/L. Namun ekstrak daun kipahit dengan konsentrasi 75 g/L tidak berbeda efektifitasnya dengan ekstrak daun kipahit dengan konsentrasi 50 g/L. Kata Kunci: Ekstrak

  17. Selection of sugar-beet calli to obtain plants resistant to Cercospora beticola

    International Nuclear Information System (INIS)

    The classic double-layer technique used in microbiology was tentatively adapted to select sugar-beet calli resistant to different toxins of Cercospora beticola. For this purpose, calli were placed on a culture medium containing benomyl, which was poured on the C. beticola culture. Among the different fungal species studied (C. musae, C. medicaginis, C. carotae and C. beticola), only C. beticola and C. carotae induced a toxic effect, which was unrelated to the ability of the fungal strains to produce the known toxins cercosporin and/or C. beticola toxin. The toxic preparation, which causes the death of beet calli, also induced a loss of electrolytes from sugar-beet leaf discs. (author)

  18. Adubação orgânica, nutrição e progresso de cercosporiose e ferrugem-do-cafeeiro Organic fertilization, nutrition and the progress of brown eye spot and rust in coffee trees

    OpenAIRE

    Florisvalda da Silva Santos; Paulo Estevão de Souza; Edson Ampélio Pozza; Júlio César Miranda; Eudes de Arruda Carvalho; Luiz Henrique Monteiro Fernandes; Adélia Aziz Alexandre Pozza

    2008-01-01

    O objetivo deste trabalho foi avaliar o efeito de fontes nutricionais orgânicas, no progresso da cercosporiose e da ferrugem-do-cafeeiro, entre novembro/2003 e novembro/2005. Utilizou-se o delineamento de blocos ao acaso, com quatro repetições e seis tratamentos: palha de café + chorume suíno (PC+CS); torta de mamona + crotalária (TM+Cr); palha de café (PC); esterco bovino + crotalária (EB+Cr); palha de café + torta de mamona (PC+TM); e composto orgânico, torta de mamona, chorume suíno e crot...

  19. Uji Antagonisme Jamur Endofit Dari Tanaman Padi Terhadap Cercospora oryzae Miyake dan Curvularia lunata (Wakk) Boed. di Laboratorium

    OpenAIRE

    Manurung, Ida Rumia

    2015-01-01

    Ida Rumia Manurung. 2014. “Antagonism Test Between Endophytic Fungi From Rice Against Cercospora oryzae Miyake and Curvularia lunata (Wakk) Boed. in Laboratory”, supervised by Mukhtar Iskandar Pinem and Lahmuddin Lubis. This research aimed to know antagonism ability of endophytic fungi to control C. oryzae and C. lunata in laboratory. This research was held at Plant Disease Laboratory, Agroecotechnology Program Study, Faculty of Agriculture, University of Sumatera Utara with ...

  20. Response of sugar beet plants to ultraviolet-B (280-320 nm) radiation and Cercospora leaf spot disease

    International Nuclear Information System (INIS)

    Sugar beet (Beta vulgaris L.) plants injected with Cercospora beticola Sacc. as well as non-infected plants were grown under visible light with or without ultraviolet-B (UV-B, 280-320 nm) radiation for 40 days. An interaction between UV-B radiation and Cercospora leaf spot disease was observed, resulting in a large reduction in leaf chlorophyll content, dry weight of leaf laminae, petioles and storage roots. Lipid peroxidation in leaves also increased the most under the combined treatments. This was also true for ultraweak luminescence from both adaxial and abaxial leaf surfaces. However, no correlation between lipid peroxidation and ultraweak luminescence was observed. Ultraviolet-B radiation given alone appeared to have either a stimulating effect, giving an increase in dry weight of laminac and reducing lipid peroxidation, or no effect. This lack of effect was seen in the absence of change in dry weight of storage roots and chlorophyll content relative to controls. The study demonstrated a harmful interaction between UV-B radiation and Cercospora leaf spot disease on sugar beet

  1. Herencia de la resistencia al complejo Cercospora spp. en líneas de maíz tropical

    Directory of Open Access Journals (Sweden)

    Narro L. Luis

    2005-09-01

    Full Text Available Se evaluó un diseño dialélico (Griffing Modelo 1 entre 10 líneas endogámicas de maíz tropical contrastantes en su reacción a Cercospora spp. (5 tolerantes y 5 susceptibles, en tres localidades en lotes bajo siembra directa, con presencia natural del patógeno e inoculación artificial, para la variable índice de enfermedad (escala 1 a 5. En el análisis de varianza, por localidad y combinado, los efectos de habilidad combinatoria general (hcg, habilidad combinatoria específica (hce, y recíprocos resultaron altamente significativos; siendo >10 veces mayor los efectos de la hcg que los de hce, indicando predominio de la acción genética aditiva. Resultados también sugieren que la resistencia está controlada por efectos maternos o de citoplasma. La acumulación de genes de resistencia y la conformación de variedades sintéticas tolerantes es una alternativa a implementar en programas del mejoramiento de resistencia a Cercospora de maíces tropicales. ABSTRACT Inheritance of resistance to Cercospora Complex in tropical maize (zea mays inbred lines. Inheritance of resistance to the maize disease complex caused by Cercospora spp on tropical maize was studied by generating and evaluating a diallel mating model (Griffing model I using ten corn inbred lines with contrasting levels of disease reaction (5 tolerant and 5 susceptible. Parental lines and their crosses, both direct and reciprocal, were evaluated in three locations using conservation tillage and inoculated artificially with inoculum prepared using a mixture of foliar maize diseases collected from several farmer’s fields. Analysis of variance for the variable index of disease (scale 1 to 5, both locality and combined, the general combining ability (hcg, specific combining ability (hce, and reciprocal effects were highly significant, with the hcg portion being >10 times greater than the hce component, indicating that the genetic action is predominantly additive. Data

  2. Influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina

    Directory of Open Access Journals (Sweden)

    Diana Erica Gómez

    2013-06-01

    Full Text Available Fungi require special substrates for their isolation, vegetative growth and sporulation. In experiments conducted in the laboratory, the influence of substrates, light, filter paper and pH on the sporulation of Cercospora sojina conidia, the causal agent of soybean frogeye leaf spot, was assessed. The media potato sucrose agar, V-8 agar, tomato extract agar, soybean leaf extract agar, soybean seed extract agar, soybean meal agar, soybean flour agar and wheat flour agar were tested, added on the surface, with and without filter paper and under two light regimes, with 12 h light at 25°± 2°C and in the dark. A triple factorial 8x2x2 (substrates x light/dark x with/without filter paper design with four replicates was used. V-8 agar medium was employed and the pH was adjusted with HCl 0.1N or NaOH 0.1N before autoclaving to the values: 3, 4, 5, 6, 7 and 8, and the pH of V-8 agar medium is 6.7. The evaluation was done on the seventh day of incubation. Data underwent regression analysis. Sporulation was maximized on the agar media V-8, seed extract, oat flour, tomato extract, and potato sucrose in the presence of filter paper and 12h light. On V-8 medium, maximal sporulation was obtained with pH 6.7.

  3. Adubação orgânica, nutrição e progresso de cercosporiose e ferrugem-do-cafeeiro Organic fertilization, nutrition and the progress of brown eye spot and rust in coffee trees

    Directory of Open Access Journals (Sweden)

    Florisvalda da Silva Santos

    2008-07-01

    Full Text Available O objetivo deste trabalho foi avaliar o efeito de fontes nutricionais orgânicas, no progresso da cercosporiose e da ferrugem-do-cafeeiro, entre novembro/2003 e novembro/2005. Utilizou-se o delineamento de blocos ao acaso, com quatro repetições e seis tratamentos: palha de café + chorume suíno (PC+CS; torta de mamona + crotalária (TM+Cr; palha de café (PC; esterco bovino + crotalária (EB+Cr; palha de café + torta de mamona (PC+TM; e composto orgânico, torta de mamona, chorume suíno e crotalária (testemunha. A maior área abaixo da curva de progresso da incidência da cercosporiose e da ferrugem foi registrada no tratamento PC (respectivamente 62 e 38% superiores à testemunha. O maior progresso das doenças coincidiu com a elevação no teor de K e redução nos teores de Ca foliares no tratamento PC, comparado às demais fontes de adubação, e resultou em maior desfolha e menor produtividade. Os tratamentos PC+TM e PC+CS reduziram a incidência da cercosporiose em 38% e da ferrugem em 31 e 21%, respectivamente, e aumentou o teor de Ca foliar ao final da fase de granação do cafeeiro, em comparação ao tratamento PC. O equilíbrio nutricional desses cafeeiros conferiu-lhes maior resistência e reduziu o efeito da bienalidade nas safras 2003/2004 e 2004/2005.The objective of this work was to evaluate the effect of organic nutrional sources on the progress of brown eye spot and coffee rust in coffee plants, between November 2003 and November 2005. The experimental design adopted was the randomized blocks, with four replications and six treatment plots: coffee fruit peel + swine waste (CP+SW; castor bean presscake + crotalaria (CBP+Cr; coffee fruit peel (CP; bovine manure + crotalaria (BM+Cr; coffee fruit peel + castor bean presscake (CP+CBP; and standard fertilization used in the farm, which combines compost, castor bean presscake, swine waste and crotalaria (control. The higher area under the incidence progress curves of brown eye

  4. Crescimento, esporulação e virulência do inóculo de Cercospora piaropi, agente de biocontrole do aguapé Growth, sporulation and virulence of Cercospora piaropi inoculum, biocontrol agent of water hyacinth

    Directory of Open Access Journals (Sweden)

    Zilá R. de Ávila

    2004-04-01

    Full Text Available O presente ensaio foi realizado com o objetivo de avaliar a produção de biomassa micelial bem como a esporulação de Cercospora piaropi, nos meios líquidos V8, ETD (Extrato de Tomate Diluído e BD (Batata - Dextrose, em períodos de cultivo de 96, 120, 144 e 168 h, sob agitação constante. Adicionalmente foi avaliado o efeito de períodos de desidratação da biomassa micelial (24, 48, 72, 96 e 120 h sobre a esporulação. Os inóculos obtidos foram avaliados quanto à severidade da doença em plantas de aguapé (Eichhornia crassipes. De acordo com os resultados, o meio ETD proporcionou maior crescimento micelial em relação aos meios BD e V8, destacando-se o período de 144 h de agitação. Entretanto, o meio V8 induziu esporulação superior do patógeno, quando cultivado por 120 h. Os inóculos obtidos nos meios V8 e ETD causaram maiores valores de severidade da doença. O período de desidratação da biomassa micelial a partir de 72 h favoreceu maior produção de conídios. Não houve efeito do período de desidratação sobre a severidade da doença.The current assay was carried out to evaluate the mycelial biomass production and sporulation of Cercospora piaropi in V8, ETD and BD liquid media at incubation periods of 96, 120, 144, and 168 h, under constant agitation. Additionally, the effect of dehydration periods on the sporulation of mycelial biomass at 24, 48, 72, 96 and 120 h was evaluated. The obtained inocula were evaluated according to the severity of the disease on water hyacinth (Eichhornia crassipes plants. In accordance with the results, the ETD media yielded more mycelial growth than the BD and V8 media, especially after 144 h of agitation. However, the V8 medium induced a higher pathogen sporulation rate, when cultivated for up to 120 h. The obtained inocula produced in V8 and ETD media caused higher values of disease severity. The dehydration period of the mycelial biomass from 72 h was favorable for a higher conidial

  5. Inibição do crescimento micelial de Cercospora calendulae Sacc. por extratos de plantas medicinais

    Directory of Open Access Journals (Sweden)

    J.M. Nascimento

    2013-01-01

    Full Text Available O uso de extratos e óleos essenciais de plantas medicinais tem sido amplamente estudado no controle de doenças de plantas. O objetivo da realização do presente trabalho foi avaliar o efeito de extratos de Ruta graveolens L., Mentha x villosa, Calendula officinalis L., Momordica charantia L., Symphytum officinale L., Ageratum conyzoides L. e Ricinus comunis L., nas concentrações de 0, 250, 500, 1000, 2000, 5000 e 10000 mg L-1, sobre a inibição do crescimento micelial de Cercospora calendulae Sacc. in vitro. Os extratos foram obtidos por infusão. O experimento foi desenvolvido no Laboratório de Fitopatologia da FCA/UFGD, estado de Mato Grosso do Sul, em delineamento inteiramente casualizado, esquema fatorial 7 extratos x 7 concentrações, com seis repetições. Foi detectado efeito dos extratos e suas concentrações sobre o crescimento do fungo, sendo a interação significativa. Os extratos de calêndula, arruda, hortelã e melão de São Caetano, nas maiores concentrações resultaram em maiores porcentagens de inibição, próximas de 100%, 30%, 35% e 40%, respectivamente, a 10000 mg L-1.

  6. RNA-sequencing of Cercospora beticola DMI-sensitive and -resistant isolates after treatment with tetraconazole identifies common and contrasting pathway induction.

    Science.gov (United States)

    Bolton, Melvin D; Ebert, Malaika K; Faino, Luigi; Rivera-Varas, Viviana; de Jonge, Ronnie; Van de Peer, Yves; Thomma, Bart P H J; Secor, Gary A

    2016-07-01

    Cercospora beticola causes Cercospora leaf spot of sugar beet. Cercospora leaf spot management measures often include application of the sterol demethylation inhibitor (DMI) class of fungicides. The reliance on DMIs and the consequent selection pressures imposed by their widespread use has led to the emergence of resistance in C. beticola populations. Insight into the molecular basis of tetraconazole resistance may lead to molecular tools to identify DMI-resistant strains for fungicide resistance management programs. Previous work has shown that expression of the gene encoding the DMI target enzyme (CYP51) is generally higher and inducible in DMI-resistant C. beticola field strains. In this study, we extended the molecular basis of DMI resistance in this pathosystem by profiling the transcriptional response of two C. beticola strains contrasting for resistance to tetraconazole. A majority of the genes in the ergosterol biosynthesis pathway were induced to similar levels in both strains with the exception of CbCyp51, which was induced several-fold higher in the DMI-resistant strain. In contrast, a secondary metabolite gene cluster was induced in the resistance strain, but repressed in the sensitive strain. Genes encoding proteins with various cell membrane fortification processes were induced in the resistance strain. Site-directed and ectopic mutants of candidate DMI-resistance genes all resulted in significantly higher EC50 values than the wild-type strain, suggesting that the cell wall and/or membrane modified as a result of the transformation process increased resistance to tetraconazole. Taken together, this study identifies important cell membrane components and provides insight into the molecular events underlying DMI resistance in C. beticola. PMID:27112724

  7. Limiares térmicos para a germinação de conídios de Cercospora sojina em dois regimes luminosos Temperature threshold for Cercospora sojina conidium germination under two light regimens

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    Juliane Nicolodi Camera

    2013-03-01

    Full Text Available O objetivo desse trabalho foi avaliar a germinação de conídios horas, verificou-se que a maior germinação ocorreu na temperatura de Cercospora sojina em diferentes temperaturas com luz contínua de 23,8 ºC, para 6 horas foi de 24,3 ºC, para 9 horas foi de 22,9 ºC e no escuro, visando a determinar os limiares térmicos inferior e e para 12 horas foi de 22,4ºC. Quando as placas foram incubadas superior e a temperatura ótima. Os conídios foram depositados na no escuro, verificou-se que a maior germinação dos conídios para superfície das placas de petri contendo substrato ágar-água. Em o tempo de exposição de 3 horas foi na temperatura de 23,8 ºC, seguida foram incubadas em DBO com luz contínua e no escuro para 6 horas foi de 23,4 ºC, para 9 horas foi de 22,9 ºC e para 12 nas temperaturas (0; 5; 10; 15; 20; 25; 30; 35 e 40 ºC e retiradas horas 22,7 ºC. A temperatura ótima para a germinação de conídios nos tempos de exposição (3, 6, 9 e 12 horas. Para os conídios de na luz contínua foi de 22,4 ºC e no escuro de 23 ºC, sendo o limiar C. sojina submetidos à luz contínua no tempo de exposição de 3 térmico inferior de 0 ºC e o superior de 40 ºC.The aim of this study was to evaluate the germination of exposure time of 3 hours, the best germination occurred at a Cercospora sojina conidia at different temperatures under continuous temperature of 23.8 ºC, for 6 hours at 24.3 ºC, for 9 hours at 22.9 ºC light and in the dark in order to determine the lower and the upper and for 12 hours at 22.4 ºC. When the plates were incubated in the temperature threshold, as well as the optimum temperature. Conidia dark, conidium germination was greater when exposure time was 3 were deposited on the surface of Petri plates containing agar-water hours at a temperature of 23.8 ºC 6 hours at 23.4 ºC, 9 hours at 22.9 substrate. They were then incubated in BOD under continuous light ºC and 12 hours at 22.7 ºC. The optimum

  8. In-vitro studies of some selected botanicals and baubiofungicide on mycelial growth and conidial germination of Cercospora arachidicola and Cercosporidium personatum

    Directory of Open Access Journals (Sweden)

    M.H. Hossain

    2013-12-01

    Full Text Available Efforts have been made to assess some plant extracts namely, Lycopersicon esculentum, Tagetus patula, Achras sapota, Azadirachta indica, Datura metel, Cymbopogon citrates, Polyalthia longifolia, Allium sativum and Allium cepa in vitro for the management of leaf spot (tikka disease of groundnut cultivar Dhaka-1 caused by Cercospora arachidicola and Cercosporidium personatum. Results indicated that all the tested plant extracts and BAUBiofungicide suppressed the growth of mycelium and inhibition of conidial germination of C. arachidicola and C. personatum. Among the treatments, the leaf extracts of L. esculentum showed the most effective followed by leaf extract of D. metel, A. indica and BAU-Biofungicide in case of mycelial growth and conidial germination. Other plant extracts also had inhibitory effects. In case of conidial germination and germination inhibition, the least effective plant extract was C. citrates. Leaf extract of A. sapota was the least effective in case of mycelial growth.

  9. Analyses of expressed sequence tags from the maize foliar pathogen Cercospora zeae-maydis identify novel genes expressed during vegetative, infectious, and reproductive growth

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    Kema Gert HJ

    2008-11-01

    Full Text Available Abstract Background The ascomycete fungus Cercospora zeae-maydis is an aggressive foliar pathogen of maize that causes substantial losses annually throughout the Western Hemisphere. Despite its impact on maize production, little is known about the regulation of pathogenesis in C. zeae-maydis at the molecular level. The objectives of this study were to generate a collection of expressed sequence tags (ESTs from C. zeae-maydis and evaluate their expression during vegetative, infectious, and reproductive growth. Results A total of 27,551 ESTs was obtained from five cDNA libraries constructed from vegetative and sporulating cultures of C. zeae-maydis. The ESTs, grouped into 4088 clusters and 531 singlets, represented 4619 putative unique genes. Of these, 36% encoded proteins similar (E value ≤ 10-05 to characterized or annotated proteins from the NCBI non-redundant database representing diverse molecular functions and biological processes based on Gene Ontology (GO classification. We identified numerous, previously undescribed genes with potential roles in photoreception, pathogenesis, and the regulation of development as well as Zephyr, a novel, actively transcribed transposable element. Differential expression of selected genes was demonstrated by real-time PCR, supporting their proposed roles in vegetative, infectious, and reproductive growth. Conclusion Novel genes that are potentially involved in regulating growth, development, and pathogenesis were identified in C. zeae-maydis, providing specific targets for characterization by molecular genetics and functional genomics. The EST data establish a foundation for future studies in evolutionary and comparative genomics among species of Cercospora and other groups of plant pathogenic fungi.

  10. PR Enzyme Activities of Cercospora theae Causing Bird’s Eye Spot Disease in Tea Plants (Camellia sinensis (L. O.kuntze

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    Rajendran Yazhini

    2011-01-01

    Full Text Available A total of three isolates of Cercospora theae namely KC10, MC24 and VC38 obtained from birds eye spot disease were used for the present study and subjected to various Pathogen Related (PR enzymatic secretion by the pathogen and the plant as a response to the disease attack. The enzyme secreted from the pathogen includes CMCase, amylase, invertase, pectinase and protease, its activity was studied at various incubation periods ranging from 15, 30, 60, 90 and 120 min. All enzymes except amylase, exhibited its maximal activity at 30 min incubation period whereas amylase exhibited its optimal activity at 60 min of incubation. The study revealed that C. theae was capable of producing the range of pathogenic enzymes and exhibited its maximal activity at 10th day harvest. The said enzyme activities was also studied in healthy and C. theae infected tea leaves including chitinase, DNase, Phenylalanine Ammonia Lyase (PAL, Tyrosine Ammonia Lyase (TAL, polyphenol oxidase (PPO, peroxidase (PO and catalase in order to detect the level of defensive response. The significance of the specific enzymatic activity detected in C. theae isolates and tea leaves were discussed by correlating to the plant pathogenesis. This study showed that phyopathogenic isolates of C. theae is capable of producing various carbohydrases, pectinases and protease and defensive enzymes along with phenolic and oxidative enzymes. The significance of correlating the enzymatic activity with the virulence of the isolates was ascertained and discussed. The enzyme profile studies of the pathogenic isolates assist in understanding and revealing of host-pathogen interaction.

  11. Limiares térmicos para a germinação de conídios de Cercospora sojina em dois regimes luminosos

    Directory of Open Access Journals (Sweden)

    Juliane Nicolodi Camera

    2013-03-01

    Full Text Available O objetivo desse trabalho foi avaliar a germinação de conídios horas, verificou-se que a maior germinação ocorreu na temperatura de Cercospora sojina em diferentes temperaturas com luz contínua de 23,8 ºC, para 6 horas foi de 24,3 ºC, para 9 horas foi de 22,9 ºC e no escuro, visando a determinar os limiares térmicos inferior e e para 12 horas foi de 22,4ºC. Quando as placas foram incubadas superior e a temperatura ótima. Os conídios foram depositados na no escuro, verificou-se que a maior germinação dos conídios para superfície das placas de petri contendo substrato ágar-água. Em o tempo de exposição de 3 horas foi na temperatura de 23,8 ºC, seguida foram incubadas em DBO com luz contínua e no escuro para 6 horas foi de 23,4 ºC, para 9 horas foi de 22,9 ºC e para 12 nas temperaturas (0; 5; 10; 15; 20; 25; 30; 35 e 40 ºC e retiradas horas 22,7 ºC. A temperatura ótima para a germinação de conídios nos tempos de exposição (3, 6, 9 e 12 horas. Para os conídios de na luz contínua foi de 22,4 ºC e no escuro de 23 ºC, sendo o limiar C. sojina submetidos à luz contínua no tempo de exposição de 3 térmico inferior de 0 ºC e o superior de 40 ºC.

  12. Effects of 12 beticolins, Cercospora beticola toxins, on proliferation of ras-transformed adrenocortical cell%真菌Cercospora beticola的12种毒素贝第高林对癌基因ras转导的肾上腺细胞生长的作用

    Institute of Scientific and Technical Information of China (English)

    丁国庆; Gabrielle MAUME; Hanan OSMAN; Martine PADIEU; Marie-Louise MILAT; Claude HUMBERT; Jean-Pierre BLEIN; Bernard F MAUME

    2001-01-01

    AIM: To explore different effects of 12 beticolins, Cercospora beticola toxins, on ras-transformed adrenocortical cell growth inhibition and their functional mechanism.METHODS: Beticolin-induced inhibition was measured with survival cell number determined by an automated photocolorimetric method. The penetration of beticolin was examined by confocal microscopy. Ras protein determined by Lowry method were separated by 14 % SDS-PAGE and electroblotted to Immobilon-P transfer membrane and detected with pan-Ras (Ab-3) monoclonal antibody. The Ca2+ chelation by beticolin was investigated using a calcium ionophore. RESULTS: Cell growth inhibition was found dose- and time-dependently at submicromolar level for beticolin-1, -2, and -13 (IC50 ≤250 nmol/L) and for beticolin-0, 6, and-11 (400 nmol/L < IC50 ≤ 500 nmol/L ). The inhibition by beticolin-1 was immediate, independent of cell culture step and not reversible for 3-day treatment. Beticolin-3 and -4 were slightly active (1 μmol/L < IC50 ≤ 2μmol/L) and beticolin-7, -9, -12, and -5 were inactive at micromolar level. The beticolin-induced cell growth inhibition was correlated with the hydrophobicity of these compounds. Beticolin-1 fluorescence in RTAC cells was detected by confocal microscopy whereas beticolin-3 and -12 were not even after a 24 h incubation period.Beticolin-1-induced cell growth inhibition was partially reverted by calcium ionophore suggesting a role of intracellular Ca2 + chelation by beticolin-1 on cell growth inhibition. Furthermore, beticolin-1 blocked up Ras p21 translocation to membrane and induced accumulation of Ras in the cytosol as an inactive form by different ways.

  13. Efeito do tipo de substrato e da presença de adubação suplementar sobre o crescimento vegetativo, nutrição mineral, custo de produção e intensidade de cercosporiose em mudas de cafeeiro formadas em tubetes Effects of substrate type and supplementary fertilization on vegetative growth, mineral nutrition, production costs, and brown eye spot of coffee seedlings in stiff plastic containers

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    Adélia Aziz Alexandre Pozza

    2007-06-01

    Full Text Available A utilização de tubetes para produzir mudas de cafeeiro (Coffea arabica L. tornou-se uma realidade. Objetivando avaliar o uso de substratos adubados ou não com fertilizante de liberação gradual e seus efeitos na nutrição, na formação, na cercosporiose e nos custos de produção de mudas de cafeeiro em tubetes, conduziu-se um experimento em viveiro, em esquema fatorial (4x2 com quatro substratos combinados com duas adubações, tendo quatro repetições. As parcelas foram constituídas por 16 tubetes de 120 mL, considerando-se como plantas úteis as seis centrais. Os tratamentos consistiram dos substratos S1= Substrato comercial I, S2 = Substrato comercial II, S3 = Substrato de eucalipto e S4 = 80% esterco de curral + 20% terra de subsolo, todos com e sem adubação com fertilizante de liberação gradual. No geral, os melhores substratos para formação de mudas de cafeeiro em tubetes foram os substratos não comerciais ou orgânicos, independentemente de receberem ou não adubação suplementar. Observou-se redução da cercosporiose com a nutrição mineral, principalmente com o cálcio. Com a utilização de todos os substratos houve ganho econômico em relação ao custo por milheiro de mudas. A maior economia foi obtida com o substrato S4, seguida do substrato S3, com melhor relação custo/benefício.The utilization of stiff plastic containers for producing coffee seedlings (Coffea arabica L. is a reality. Aiming to evaluate the effects of substrate type enriched or not with slow-release fertilizer on vegetative growth, mineral nutrition, production cost and, brown eye spot of coffee seedlings, an experiment was conducted in the plant nursery. The experimental design was a factorial scheme with four substrates in the presence and absence of supplementary fertilization, with four replicates. The plots were made of 16 containers of 120 ml, using six central plants for evaluation. The treatments consisted of S1 = Commercial Substrate

  14. Freqüência de ocorrência de agentes etiológicos, sintomas e origem de amostras do cafeeiro catalogados em 12 anos de clínica fitossanitária da UFLA Origin of coffee samples, frequency of ocurrence of ethiological agents and sympton in twelve years of the plant disease clinic at UFLA

    OpenAIRE

    Daniel Garcia Júnior; Edson Ampélio Pozza; Paulo Estevão de Souza; Viviane Talamini; Adélia Aziz Alexandre Pozza; Hilário Antônio de Castro; Ricardo Magela de Souza; Mário Sobral de Abreu; Ludwig Heinrich Pfenning

    2003-01-01

    Situada no sul de Minas Gerais, principal região cafeeira do país, a Clínica Fitossanitária da Universidade Federal de Lavras tem auxiliado os produtores, dando suporte na diagnose de doenças de origem biótica e abiótica, de modo a gerar subsídios para minimizar as perdas na produção. Nos últimos doze anos, foram analisadas 378 amostras de café. Em 78,6% das amostras, foram detectados fungos, destacando-se Colletotrichum sp. (29%), Rhizoctonia solani (18%), Cercospora coffeicola (13%), Phoma ...

  15. Diagnóstico e quantificação de doenças fúngicas da acerola no Estado da Paraíba Diagnostic and quantification of barbados cherry diseases in the State of Paraíba, Brazil

    OpenAIRE

    Fernandes A. Almeida; Egberto Araújo; Hermes Gonçalves Junior; Artur F. Barreto; Renato A. G. Carvalho

    2003-01-01

    Objetivando o diagnóstico e quantificação das doenças da acerola (Malpighia emarginata) no Estado da Paraíba, realizou-se um levantamento nas microrregiões do Sertão, Curimatau, Brejo e Litoral. As doenças identificadas foram a cercosporiose (Cercospora sp.), fusariose (Fusarium oxysporum), fumagina (Capnodium sp.), antracnose (Colletotrichum gloeosporioides) e mancha de Alternaria (Alternaria sp.). Além dessas, no teste de patogenicidade, verificou-se o desenvolvimento de lesões circulares, ...

  16. Research methodology for maize gray leaf spot:Ⅰ.sporulation of Cercospora zeae-maydis%玉米灰斑病研究方法:Ⅰ.玉米灰斑病菌孢子的培养

    Institute of Scientific and Technical Information of China (English)

    赵正龙; 吴兴兴; 毛自朝; 何月秋

    2011-01-01

    为了明确玉米灰斑病菌的产孢条件,将玉米灰斑病菌接种到马铃薯蔗糖(PSA),燕麦番茄碳酸钙(OTCA)、玉米叶粉碳酸钙(MLPCA)、燕麦粉(OA)、粗面粉(CWPA)等5种培养基上,于25℃下培养,测定该菌生长速度、产孢时间和产孢量.结果表明,菌丝在上述培养基上生长速度均较慢,每天平均生长0.33 nun左右,其中以在OA和PSA上生长速度相对较快.基于上述结果,构建了玉米灰斑病菌孢子培养的二级分段培养法,即将菌丝块接种于加有10~15粒直径为5 mm玻璃珠的马铃薯蔗糖(PS)液体培养基三角瓶里,在25℃,180 r/min条件下振荡培养15 d,形成菌丝悬液,再取菌丝悬浮液涂布在MLPCA培养基上,避光培养13 d左右,可获得大童供接种的分生孢子.长期光照促进菌丝生长,不利于孢子的形成,而长期黑暗不利于菌丝生长,但有利于孢子的形成.%The pathogen of maize gray leaf spot, Cercospora zeae-maydis, was inoculated on potato sucrose agar (PSA), oat tomato plus CaCO3 agar(OTCA), maize leaf powder plus CaCO3 agar(MLPCA), oatmeal agar(OA) and crude wheat powder agar (CWPA), and incubated at 25 ℃ for measuring the mycelial growth, sporulation time and number. The results showed that the mycelia grew very slow on the media, with an average of 0.33 mm per day. They grew fastest on OA and PSA, slower on CWPA and most slowly on MLPCA. Based on the results,a sporulation method was set up, namely two-step culturing method. The mycelia were inoculated into flask with PS and 10-15 glass balls of 5mm diameter, and shaken at 25 ℃ and 180 r/min for 15 days, to culture the mycelial fragment suspension. Then the suspension was smeared on MLPCA medium and incubated for about 13 days at 25 ℃ without light. By this way, a large number of spores could be produced for inoculation. Long illumination promoted the mycelial growth, but on the contrary, long darkness was conducive to sporulation.

  17. Main Diseases and Pests of Coffee and Their Prevention and Control in Yunnan%云南咖啡主要病虫害及防治调查研究①

    Institute of Scientific and Technical Information of China (English)

    刘树芳; 金桂梅; 杨艳鲜; 许叁卫

    2014-01-01

    对云南保山、德宏、普洱等地咖啡病虫害开展调查研究,结果表明院咖啡锈病(Hemileia vastatrix Berk et Br.)、炭疽病(Colletotrichum coffeanum Noack)、褐斑病(Cercospora coffeicola Berk. et Cooke)、幼苗立枯病(Rhizoctonia solani Kuhn.)、旋皮天牛(Acalolepta cervina Hope)、灭字虎天牛(Xylotrechus quadripes Chevr)和根粉蚧(Planococus lilacinus Cockrell)是云南咖啡主要病虫害,其危害较重。根据调查研究结果,提出相应防治方法,为云南咖啡主要病虫害的防治提供依据。%As increasing in cultivated area of coffee and its occurrence of diseases and insects in Yunnan, the study on diseases and insects of coffee and their prevention and control has important significance on sustainable production of coffee. The diseases and insects of coffee were investigated in Baoshan, Dehong, Puer cities in Yunnan of China from 2012-2013. The results showed that Hemileia vastatrix Berk et Br., Colletotrichum coffeanum Noack, Cercospora coffeicola Berk. et Cooke, Rhizoctonia solani Kuhn., Acalolepta cervina Hope, Xylotrechus quadripes Chevr, Planococus lilacinus Cockrell were the main insects and diseases and resulted into higher damage for coffee in Yunnan. According to occurrence and damage of coffee diseases and insects, some corresponding prevention and control methods have been proposed. All these will provide scientific basis for Yunnan coffee production.

  18. Freqüência de ocorrência de agentes etiológicos, sintomas e origem de amostras do cafeeiro catalogados em 12 anos de clínica fitossanitária da UFLA Origin of coffee samples, frequency of ocurrence of ethiological agents and sympton in twelve years of the plant disease clinic at UFLA

    Directory of Open Access Journals (Sweden)

    Daniel Garcia Júnior

    2003-02-01

    Full Text Available Situada no sul de Minas Gerais, principal região cafeeira do país, a Clínica Fitossanitária da Universidade Federal de Lavras tem auxiliado os produtores, dando suporte na diagnose de doenças de origem biótica e abiótica, de modo a gerar subsídios para minimizar as perdas na produção. Nos últimos doze anos, foram analisadas 378 amostras de café. Em 78,6% das amostras, foram detectados fungos, destacando-se Colletotrichum sp. (29%, Rhizoctonia solani (18%, Cercospora coffeicola (13%, Phoma sp. (13% e Fusarium sp (11%. Ocorreram ainda em números representativos casos de fitotoxidez, deficiência de nutrientes e problemas no sistema radicular. Devido à localização da Clínica, a maior parte das amostras foi proveniente de localidades na região sul do Estado (63%, seguido do Triângulo Mineiro (12% e Zona da Mata (10%.The Phytossanitary Clinic of the Federal University of Lavras, situated in the south of the State of Minas Gerais has given support to coffee producers providing diagnoses of biotic and abiotic diseases, to generate subsidies to minimize losses in production of this crop. During the last twelve years, 378 samples have been analyzed. Fungi were detected in 78,6% of the samples. The major fungi found were Colletotrichum sp. (29%, Rhizoctonia solani (18%, Cercospora coffeicola (13%, Phoma sp. (13%, e Fusarium sp (11%. Other incidences were caused by phytotoxity, mineral deficiency and bad formation of the roots. Due to the location of the Phytossanitary Clinic, most of the samples came from locations of South of Minas Gerais (63%, followed by Triângulo Mineiro (12% and Zona da Mata (10%.

  19. Incidência de pragas e doenças em agroecossistemas de café orgânico de agricultores familiares em Poço Fundo-MG Incidence of plagues and diseases in agroecossystems of organic coffee of familiar farmers in Poço Fundo-MG

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    Márcia Martins

    2004-12-01

    Full Text Available Objetivando-se determinar a incidência de pragas Leucoptera coffeella (GUÉRIN-MENÉVILLE, 1842 e Hypothenemus hampei (FERRARI, 1867 e doenças Hemileia vastatrix (BERK e BROOME, 1869 e Cercospora coffeicola (BERK e CURTIS, 1880, durante o período de janeiro de 2001 a dezembro de 2002, foram selecionados três agroecossistemas com produção de café orgânico conduzidos por agricultores familiares do município de Poço Fundo-MG. O agroecossistema I possui 2.200 pés da cultivar 'Catuaí Vermelho' em espaçamento 2,5 x 1,3 m (0,72 ha. O agroecossistema II possui 2.000 pés ('Catuaí Vermelho' em espaçamento 2,8 x 1,0 m (0,56 ha. O agroecossistema III possui 1.100 pés ('Icatu Amarelo' em espaçamento 3,0 x 0,9 m (0,36 ha. A determinação da incidência de L. coffeella, C. coffeicola e H. vastatrix foi realizada mediante levantamentos mensais. A determinação da infecção por C. coffeicola em frutos foi realizada nos meses de abril, maio e junho de 2001 e 2002. O levantamento da infestação por H. hampei foi realizado nos meses de janeiro a junho/julho de cada ano. Determinou-se que a infestação por L. coffeella ultrapassou 20% no terço superior (principalmente no período seco. A infestação por H. hampei atingiu o nível de dano somente no agroecossistema I, em 2001, e no agroecossistema III, em 2002. A infecção por H. vastatrix no agroecossistema III não atingiu nível de dano em conseqüência da tolerância da cultivar ('Icatu' à infecção por esse fungo; porém, nos agroecossistemas I e II ('Catuaí Vermelho', a infecção na lavoura ultrapassou o nível de 10% (principalmente no período seco. A infecção por C. coffeicola em folhas e frutos atingiu níveis elevados (período seco. A produtividade do agroecossistema I, em 2001, foi de 510 Kg ha-1 e, em 2002, de 2.340 Kg ha-1; no agroecossistema II, em 2001, foi de 420 Kg ha-1 e, em 2002, de 1.290 Kg ha-1; e, no agroecossistema III, foi praticamente zero, em 2001, e em

  20. Antifungal activity using medicinal plant extracts against pathogens of coffee tree

    Directory of Open Access Journals (Sweden)

    J.L. Silva

    2014-09-01

    Full Text Available Generally, the medicinal plants have antifungal substances that can be used for the plant protection against phytopathogens. The objective of this study was to know the efficiency of aqueous extracts from medicinal plants against the major etiological agents of coffee tree. The aqueous extracts used were extracted from bulbs of Allium sativum, leaves of Vernonia polysphaera, Cymbopogon citratus, Cymbopogon nardus, Cordia verbenacea, Eucalyptus citriodora, Ricinus communis, Azadirachta indica, Piper hispidinervum and flower buds of Syzygium aromaticum. The etiological agents considered for this study were Cercospora coffeicola, Colletotrichum gloeosporioides, Fusarium oxysporum, Phoma tarda, Rhizoctonia solani and Hemileia vastatrix. The screening for harmful extracts was done based on mycelial growth and conidial germination inhibition. All experiments performed were in vitro conditions. The inhibition of mycelial growth was performed mixing the extracts with the PDA. This mixture was poured in Petri dishes. On the center of the dishes was added one PDA disc with mycelium. It was incubated in a chamber set to 25ºC. The evaluation was done daily by measuring the mycelial growth. The germination assessment was also performed with Petri dishes containing agar-water medium at 2%. These were incubated at 25ºC for 24 hours. After this period the interruption of germination was performed using lactoglycerol. The experiments were conducted in a completely randomized design. The most effective plant extracts against the micelial growth and conidial germination were V. polysphaera, S. aromaticum and A. sativum.

  1. Rapid detection and quantification of Cercospora beticola in soil using PCR and ELISA assays

    OpenAIRE

    Ali, Abdallah Abdel-Megid Mohamad

    2012-01-01

    The objective of this research was to develop effective methods for qualitative and quantitative detection of C. beticola in soil. The soil borne inoculum is suggested to be an important factor for disease prediction. Therefore, these methods are aimed to improve integrated pest management systems (IPM) of sugar beet. In this study two diagnostic methods, polymerase chain reaction (PCR) and Enzyme linked immunoabsorbent ...

  2. Biological characteristics of Cercospora fagopyri%荞麦褐斑病菌及其生物学特性

    Institute of Scientific and Technical Information of China (English)

    孟有儒; 李万苍; 李文明

    2004-01-01

    荞麦(Fagopyrum esculentum Moench)富含生物类黄酮、酚类、亚油酸及钙、镁、铜、铁、锌、硒和丰富的维生素等特殊营养成分,具有降血脂、降血糖,软化血管和防病治病等效果。国内大部分省区都有种植,在甘肃历来作为复种作物种植,播种面积较小。随着人民对营养要求多样化和农业种植结构调整,荞麦种植面积逐年扩大,荞麦褐斑病的发生危害愈来愈重,

  3. The heterothallic sugarbeet pathogen Cercospora beticola contains exon fragments of both MAT genes that are homogenized by concerted evolution

    NARCIS (Netherlands)

    Bolton, M.D.; Jonge, de R.; Inderbitzin, P.; Liu, Z.; Birla, K.; Peer, Van de Y.; Subbarao, K.; Thomma, B.P.H.J.; Secor, G.

    2014-01-01

    Dothideomycetes is one of the most ecologically diverse and economically important classes of fungi. Sexual reproduction in this group is governed by mating type (MAT) genes at the MAT1 locus. Self-sterile (heterothallic) species contain one of two genes at MAT1 (MAT1-1-1 or MAT1-2-1) and only isola

  4. Caractérisation des populations de Mycosphaerella fijiensis et épidémiologie de la cercosporiose noire du bananier dans la région de Kisangani, RDC

    OpenAIRE

    Onautshu Odimba, Didy; Legrève, Anne; Dhed'a Djailo, Benoît

    2013-01-01

    The black leaf streak disease (MNR) , caused by the ascomycete fungus Mycosphaerella fijiensis , is one of the most devastating diseases banana ( Musa spp.) . It is a major constraint to production , particularly in countries of the humid tropics where banana is a staple food crop . The objective of this thesis was to characterize the M. fijiensis populations and to study the epidemiology of the MNR in region of Kisangani (DRC) to evaluate protection strategies integrated to develop in crops ...

  5. Influência da nutrição mineral na intensidade da mancha-de-olho-pardo em mudas de cafeeiro Influence of the mineral nutrition on intensity of brown-eye spot in young coffee plants

    Directory of Open Access Journals (Sweden)

    Adélia Aziz Alexandre Pozza

    2001-01-01

    Full Text Available Objetivando avaliar a intensidade da mancha-de-olho-pardo do cafeeiro, variedade Catuaí Vermelho, com relação ao estado nutricional das plantas quanto a N e K, realizou-se um experimento no viveiro da Universidade Federal de Viçosa, em Viçosa, MG, utilizando solução nutritiva circulante. Empregaram-se 16 tratamentos, em delineamento inteiramente casualizado, com três repetições e duas plantas por parcela, em esquema fatorial com quatro doses de K (3, 5, 7 e 9mmol/L e quatro doses de N (3, 7, 11 e 15mmol/L. Após sete inoculações de conídios, e avaliações, colheram-se as plantas. A produção de matéria seca total, a área foliar total, e a área abaixo da curva de progresso (AACP do número total de folhas não foram influenciadas pelas doses de K, mas aumentaram com o incremento das doses de N. Observou-se elevação, na AACP, do número de lesões por folha, e na desfolha com o aumento das doses de K e a redução das doses de N. A elevação nas doses de K promoveu redução nos teores foliares de Ca e Cu. Osteores foliares de P, Mg, Mn e Fe não foram influenciados pelas doses de K e tiveram pequena redução com o aumento de N na solução, elevando-se a seguir.The experiment was carried out at the coffee nursery of the Universide Federal de Viçosa, in Viçosa, MG, Brazil, using nutrient circulating solution to evaluate the intensity of brown-eye spot (Cercospora coffeicola, variety Catuaí Vermelho, as a function of N and K. Acompletely randomized design with 16 treatments, three replicates, and two plants per plot was used in a factorial with four levels of K (3, 5, 7 and 9mmol/L combined with four levels of N (3, 7, 11 and 15mmol/L. After seven inoculations and evaluations, the plants were picked. Total dry matter production, total leaf area, and the area below the curve of progress (AACP for the total number of leaves were not influenced by the levels of K, but increased with increasing levels of N. There was increase

  6. Citronella essential oil in the control and activation of coffee plants defense response against rust and brown eye spot Óleo essencial de citronela no controle e na ativação de respostas de defesa do cafeeiro contra a ferrugem e cercosporiose

    OpenAIRE

    Ricardo Borges Pereira; Gilvaine Ciavareli Lucas; Fabiano José Perina; Pedro Martins Ribeiro Júnior; Eduardo Alves

    2012-01-01

    The rust and brown eye spot are the main coffee diseases. The losses are due to intense defoliation of plants, which has reduced its production and longevity. The brown eye spot also occurs in fruits, with negative effects on the beverage quality. Some essential oils have presented promising results in the control of plant diseases, as an alternative to the use of fungicides. The objective of this study was to evaluate citronella essential oil in the control of rust and brown eye spot and in ...

  7. Improvement of mungbean by X-ray and thermal neutron irradiation

    International Nuclear Information System (INIS)

    With the aim of improving yield, resistance to Cercospora leaf spot and pod shattering, mungbean varieties Kyunggi No. 5 and M-317 were irradiated with X-rays and thermal neutrons. High yielding mutant lines are generally characterized by a higher number of pods per plant. Better Cercospora resistance appears often associated with later maturity. Satisfactory shattering resistance was not yet obtained. (author)

  8. Assessment of aflatoxigenicAspergillusand other fungi in millet and sesame from Plateau State, Nigeria

    DEFF Research Database (Denmark)

    Ezekiel, C.N.; Udom, I.E.; Frisvad, Jens Christian; Adetunji, M.C.; Houbraken, J.; Fapohunda, S.O.; Samson, R.A.; Atanda, O.O.; Agi-Otto, M.C.; Onashile, O.A.

    2014-01-01

    . Diverse moulds includingAlternaria,Aspergillus,Cercospora,Fusarium,Mucor,Penicillium,RhizopusandTrichodermawere isolated.Aspergilluswas predominantly present in both crops (46–48%), and amongst the potentially aflatoxigenicAspergillusspecies,A. flavusrecorded the highest incidence (68% in fonio millet; 86...

  9. Alternatives agro-écologiques à l’usage des intrants chimiques dans les bananeraies plantains - Le cas de deux régions de la Caraïbe : Guadeloupe et Haïti

    OpenAIRE

    Deloné, Brunise

    2014-01-01

    La banane plantain (Musa paradisiaca) est l’aliment de base de millions de personnes dans le monde et sa culture génère des revenus permanents pour un grand nombre d’agriculteurs, dans des plantations de taille petite ou moyenne. Comme dans d’autres régions tropicales, la culture du plantain en Guadeloupe et en Haïti est soumise à de fortes contraintes parasitaires aussi bien telluriques (i.e. du sol : nématodes phytoparasites et charançon du bananier) qu’aériennes (Cercosporiose noire notamm...

  10. BUSCANDO UM BIOHERBICIDA CONTRA Cyperus sp (tiririca

    Directory of Open Access Journals (Sweden)

    Cristine Benfatti Gonzalez

    2001-01-01

    Full Text Available As an alternative to conventional control of Cyperus sp (herbaceousnutsedge, with chemical products, it rises the biological control, making use of phytopathogenic microorganisms. As results of the whole production of Cercospora henningsii e Cercospora caricis by fermentation in potato-dextrose (PD liquid medium, it was observed that both species obtained the optimum growth at 36o C. C. henningsii attained the best value after a period of 72 h of fermentation and C. caricis attained it after 60 h. The field experiments, against Cyperus sp (nutsedge, with the obtained products by liquid fermentation of Cercospora sp (C. henningsii and/or C. caribaea, andwith the use of "wet chamber", proved the pathogenic potential bioherbicide of these microorganisms. = Como alternativa ao controle convencional do Cyperus sp (tiririca usando produtos químicos, surge o controle biológico, empregando-se microrganismos fitopatógenos. Como resultados da produção massal de Cercospora henningsii eCercospora caricis através de cultivo submerso em meio de cultura batata-dextrose (BD, observou-se que ambas espécies obtiveram um crescimento ótimo a 36o C, sendo que para C. henningsii se deu com 72 h de fermentação e para C. caricis com 60 h. Os experimentos de campo, bem como os de Câmara úmida, contra Cyperus sp (tiririca,com os produtos obtidos pelo cultivo submerso de Cercospora sp (C. henningsii e/ou C. caribaea, demonstraram a patogenicidade, ou seja, o potencial bioherbicida desses microrganismos.

  11. Permanent genetic resources added to molecular ecology resources database 1 January 2009-30 April 2009

    OpenAIRE

    Abercrombie, L. G.; Anderson, C M; Baldwin, B G; Bang, I. C.; Beldade, R.; Bernardi, G.; Boubou, A.; Branca, A; Bretagnolle, F.; Bruford, M. W.; Buonamici, A.; Burnett, R. K.; Canal, D.; Cardenas, H; Caullet, C.

    2009-01-01

    This article documents the addition of 283 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Agalinis acuta; Ambrosia artemisiifolia; Berula erecta; Casuarius casuarius; Cercospora zeae-maydis; Chorthippus parallelus; Conyza canadensis; Cotesia sesamiae; Epinephelus acanthistius; Ficedula hypoleuca; Grindelia hirsutula; Guadua angustifolia; Leucadendron rubrum; Maritrema novaezealandensis; Meretrix meretrix; Nilaparvata luge...

  12. Permanent Genetic Resources added to Molecular Ecology Resources database 1 January 2009-30 April 2009.

    Science.gov (United States)

    Abercrombie, L G; Anderson, C M; Baldwin, B G; Bang, I C; Beldade, R; Bernardi, G; Boubou, A; Branca, A; Bretagnolle, F; Bruford, M W; Buonamici, A; Burnett, R K; Canal, D; Cárdenas, H; Caullet, C; Chen, S Y; Chun, Y J; Cossu, C; Crane, C F; Cros-Arteil, S; Cudney-Bueno, R; Danti, R; Dávila, J A; Della Rocca, G; Dobata, S; Dunkle, L D; Dupas, S; Faure, N; Ferrero, M E; Fumanal, B; Gigot, G; González, I; Goodwin, S B; Groth, D; Hardesty, B D; Hasegawa, E; Hoffman, E A; Hou, M L; Jamsari, A F J; Ji, H J; Johnson, D H; Joseph, L; Justy, F; Kang, E J; Kaufmann, B; Kim, K S; Kim, W J; Koehler, A V; Laitung, B; Latch, P; Liu, Y D; Manjerovic, M B; Martel, E; Metcalfe, S S; Miller, J N; Midgley, J J; Migeon, A; Moore, A J; Moore, W L; Morris, V R F; Navajas, M; Navia, D; Neel, M C; De Nova, P J G; Olivieri, I; Omura, T; Othman, A S; Oudot-Canaff, J; Panthee, D R; Parkinson, C L; Patimah, I; Pérez-Galindo, C A; Pettengill, J B; Pfautsch, S; Piola, F; Potti, J; Poulin, R; Raimondi, P T; Rinehart, T A; Ruzainah, A; Sarver, S K; Scheffler, B E; Schneider, A R R; Silvain, J F; Siti Azizah, M N; Springer, Y P; Stewart, C N; Sun, W; Tiedemann, R; Tsuji, K; Trigiano, R N; Vendramin, G G; Wadl, P A; Wang, L; Wang, X; Watanabe, K; Waterman, J M; Weisser, W W; Westcott, D A; Wiesner, K R; Xu, X F; Yaegashi, S; Yuan, J S

    2009-09-01

    This article documents the addition of 283 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Agalinis acuta; Ambrosia artemisiifolia; Berula erecta; Casuarius casuarius; Cercospora zeae-maydis; Chorthippus parallelus; Conyza canadensis; Cotesia sesamiae; Epinephelus acanthistius; Ficedula hypoleuca; Grindelia hirsutula; Guadua angustifolia; Leucadendron rubrum; Maritrema novaezealandensis; Meretrix meretrix; Nilaparvata lugens; Oxyeleotris marmoratus; Phoxinus neogaeus; Pristomyrmex punctatus; Pseudobagrus brevicorpus; Seiridium cardinale; Stenopsyche marmorata; Tetranychus evansi and Xerus inauris. These loci were cross-tested on the following species: Agalinis decemloba; Agalinis tenella; Agalinis obtusifolia; Agalinis setacea; Agalinis skinneriana; Cercospora zeina; Cercospora kikuchii; Cercospora sorghi; Mycosphaerella graminicola; Setosphaeria turcica; Magnaporthe oryzae; Cotesia flavipes; Cotesia marginiventris; Grindelia Xpaludosa; Grindelia chiloensis; Grindelia fastigiata; Grindelia lanceolata; Grindelia squarrosa; Leucadendron coniferum; Leucadendron salicifolium; Leucadendron tinctum; Leucadendron meridianum; Laodelphax striatellus; Sogatella furcifera; Phoxinus eos; Phoxinus rigidus; Phoxinus brevispinosus; Phoxinus bicolor; Tetranychus urticae; Tetranychus turkestani; Tetranychus ludeni; Tetranychus neocaledonicus; Tetranychus amicus; Amphitetranychus viennensis; Eotetranychus rubiphilus; Eotetranychus tiliarium; Oligonychus perseae; Panonychus citri; Bryobia rubrioculus; Schizonobia bundi; Petrobia harti; Xerus princeps; Spermophilus tridecemlineatus and Sciurus carolinensis. PMID:21564911

  13. No Spray' Rose Cultivars for the Mid South

    Science.gov (United States)

    Sixty cultivars of roses, most having disease resistance claims associated with their descriptions in rose catalogs were planted in Tennessee. Plants were evaluated every two weeks from planting until frost for susceptibility to black spot and Cercospora leaf spot using the following scale: 0 = no ...

  14. Fungal Planet 91 – 31 May 2011

    OpenAIRE

    Crous, P.W.; Groenewald, J.Z.; Shivas, R.G.; Edwards, J.; Seifert, K.A.; Alfenas, A.C.; Alfenas, R.F.; Burgess, T.I.; Carnegie, A.J.; Hardy, G.E.St.J.; Hiscock, N.; Hüberli, D.; Jung, T; Louis-Seize, G.; Okada, G.

    2011-01-01

    Novel species of microfungi described in the present study include the following from Australia: Bagadiella victoriae and Bagadiella koalae on Eucalyptus spp., Catenulostroma eucalyptorum on Eucalyptus laevopinea, Cercospora eremochloae on Eremochloa bimaculata, Devriesia queenslandica on Scaevola taccada, Diaporthe musigena on Musa sp., Diaporthe acaciigena on Acacia retinodes, Leptoxyphium kurandae on Eucalyptus sp., Neofusicoccum grevilleae on Grevillea aurea, Phytophthora fluvialis from w...

  15. Processing, disulfide pattern, and biological activity of a sugar beet defensin, AX2, expressed in Pichia pastoris

    DEFF Research Database (Denmark)

    Kristensen, A K; Brunstedt, J; Nielsen, J E;

    1999-01-01

    AX2 is a 46-amino-acid cysteine-rich peptide isolated from sugar beet leaves infected with the fungus Cercospora beticola (Sacc.). AX2 strongly inhibits the growth of C. beticola and other filamentous fungi, but has little or no effect against bacteria. AX2 is produced in very low amounts in suga...

  16. Doenças da vinagreira no Estado do Maranhão

    Directory of Open Access Journals (Sweden)

    Gilson Soares da Silva

    2014-12-01

    Full Text Available Neste trabalho, são descritas as principais doenças da vinagreira (Hibiscus sabdariffa que ocorrem no Estado do Maranhão. Os patógenos identificados foram Cercospora malayensis, Corynespora cassiicola, Lasiodiplodia theobromae, Meloidogyne incognita, Phytophthora nicotianae e Sclerotium rolfsii.

  17. Doenças da vinagreira no Estado do Maranhão

    OpenAIRE

    Gilson Soares da Silva; Adriano Soares Rêgo; Raycenne Rosa Leite

    2014-01-01

    Neste trabalho, são descritas as principais doenças da vinagreira (Hibiscus sabdariffa) que ocorrem no Estado do Maranhão. Os patógenos identificados foram Cercospora malayensis, Corynespora cassiicola, Lasiodiplodia theobromae, Meloidogyne incognita, Phytophthora nicotianae e Sclerotium rolfsii.

  18. Use of bacillus biocontrol agents for disease management in organic rice

    Science.gov (United States)

    Organic rice production has significantly increased in the U. S. over the last decade. Organic rice growers lack effective tools to manage sheath blight, caused by Rhizoctonia solani, and narrow brown leaf spot, caused by Cercospora janseana, two major diseases affecting organic rice production. Thi...

  19. Design, Synthesis, and Fungicidal Activities of Novel 5-Methyl-1H-1,2,3- trizole-4-carboxyl Amide Analogues.

    Science.gov (United States)

    Wang, Zhen-Jun; Yang, Hui-Hui; Tian, Lei; Zhao, Wei-Guang

    2016-01-01

    Succinate dehydrogenase inhibitors (SDHIs) are fungicides with an amide bond widely used to control plant diseases caused by phytopathogenic fungi. Because of broad spectrum activity of new SDHIs, they have attracted wide attention from the research community. A series of structurally novel SDHIs with a bioactive 1,2,3-triazole moiety were designed and synthesized. Bioactivity screening showed that some of designed N-phenethyl-1,2,3-trizole-4-carboxyl amide analogues exhibited good fungicidal activities toward Sclerotinia sclerotiorum and Botrytis cinerea, while some of Nbenzyl- 1,2,3-trizole-4-carboxyl amide analogues exhibited good fungicidal activities toward Phytophthora capsici and Cercospora arachidicola. EC50 value of compound 5d against Cercospora arachidicola (6.6 µg/mL) was lower than that of chlorothalonil (12.3 µg/mL). PMID:26558376

  20. Effect of genotype on sugar beet yield and quality

    Directory of Open Access Journals (Sweden)

    Nenadić N.

    2003-01-01

    Full Text Available The effect of a considerable number of both domestic and foreign sugar beet genotypes on root yield and quality was investigated. The data demonstrated the most favorable results of some genotypes for root yield and sugar content. Trials were conducted on rhizomania infested soil, thus tolerant genotypes were used. Susceptible cultivars represented the control. In the trial root yield was high and sugar content low. On average, in the genotypes tested, root yield varied from 73.98 to 93.30 t/ha and sugar content from 11.90 to 13.36%, depending on weather conditions. Root yield of the genotypes investigated varied from 30.61 to 112.64 t/ha and sugar content from 10.60 to 14.20%. The Swedish cultivar Dorotea (tolerant to both rhizomania and cercospora was the most yielding. The least yielding (susceptible to both rhizomania and cercospora was the domestic cultivar Dana.

  1. Discrimination of Five Citrus Diseased Leaves by FTIR Spectroscopy

    OpenAIRE

    Zhao, Xingxiang; Liu, Gang; Li, Weixing; Wang, Xiaohua; Hao, Jianming; Zhou, Xiangping

    2014-01-01

    In this paper, citrus brown spot, huanglongbing, canker, fuliginous, cercospora sp. and healthy leaves were studied using Fourier transform infrared spectroscopy (FTIR) combined with statistical analysis. The results showed that the spectra of the samples were similar, whereas there were obvious differences in the second derivatives of infrared spectra in the range of 1500-700 cm-1. The correlative analysis were evaluated, results showed that the correlation coefficients were larger than 0.91...

  2. Identification of Immunity Related Genes to Study the Physalis peruviana – Fusarium oxysporum Pathosystem

    OpenAIRE

    Enciso-Rodríguez, Felix E.; Carolina González; Rodríguez, Edwin A.; Camilo E López; David Landsman; Luz Stella Barrero; Leonardo Mariño-Ramírez

    2013-01-01

    The Cape gooseberry ( Physalis peruviana L) is an Andean exotic fruit with high nutritional value and appealing medicinal properties. However, its cultivation faces important phytosanitary problems mainly due to pathogens like Fusarium oxysporum, Cercospora physalidis and Alternaria spp. Here we used the Cape gooseberry foliar transcriptome to search for proteins that encode conserved domains related to plant immunity including: NBS (Nucleotide Binding Site), CC (Coiled-Coil), TIR (Toll/Inter...

  3. Pathogenicity of seed-borne and seedling fungi of groundnut cv. Tatu/
    Patogenicidade de fungos associados às sementes e plântulas de amendoim cv. Tatu

    OpenAIRE

    Débora Cristina Santiago; Édison Miglioranza; Romeu Munashi Endo; Nair Mieko Takaki Bellettini

    2005-01-01

    Seeds and seedlings of groundnut (Arachis hypogaea) cv. Tatu were investigated under laboratory and green house conditions to detect and identify any seed-borne pathogenic fungi present, besides determining the pathogenicity and the seed damages. The fungi detected were Aspergillus niger, Fusarium oxysporum, Cercospora arachidicola, Rhizopus sp., Penicillium sp., Cladosporium sp. and Phoma sp.. The most frequently isolated fungi were Aspergillus niger and Penicillium sp.. These fungi were tra...

  4. Bladschimmelbestrijding suikerbieten

    OpenAIRE

    Meuffels, G.J.H.M.; Maassen, Jurgen

    2010-01-01

    Het heeft geen zin om bladschimmels te bestrijden als er nog geen aantasting door de ziekten Cercospora, roest, echte meeldauw of ramularia wordt aangetroffen. IRS ontwikkelde samen met Opticrop/Agrovision een bladschimmeladviesmodel dat telers helpt om de suikerbieten op kritieke momenten te inspecteren en zo het juiste moment van spuiten nauwkeurig te bepalen. Pas als er sprake is van aantasting, wordt overgegaan tot het uitvoeren van een bestrijding. Naar verwachting spuiten telers hierdoo...

  5. Desinfektion von Gemüsesamen mit belüftetem Dampf

    OpenAIRE

    Heller, Dr. Werner E.

    2009-01-01

    Healthy seeds are a necessary condition for economic production of high quality vegetables, not only in organic production. Seed health comprises not just high levels of germination and emergence, but also absence of fungal infections. More than 200 genera of vegetable seed borne pathogenic fungi are listed in the literature: e.g. Alternaria, Ascochyta, Botrytis, Bremia, Cercospora, Colletotrichum, Peronospora, Phoma, Plasmopara, Phytophthora, Stemphylium, Septoria. Hot wate...

  6. PARASITE MYCOPOPULATION OF SOYBEAN GRAIN

    OpenAIRE

    Jasenka Ćosić; Karolina Vrandečić; Draženka Jurković; Ivan Ereš; Jelena Poštić

    2008-01-01

    Disease appearance on soybean can influence quality and quantity of yield. Different spieces of saprophyte and parasite fungi can be isolated from stems, pods and grain of soybean. The aim of the research was to evaluate the incidence of important disease on natural soybean grain over the period of 4 years (2004-2007) of experiment held on the location Sopot-Vinkovci and included 9 cultivars of soybean. The following plant pathogenic fungi were identified: Peronospora, Sclerotinia, Cercospora...

  7. Potential biochemical markers for selection of disease resistance in Vigna radiata

    International Nuclear Information System (INIS)

    The Vigna radiata (L.) Wilczek (Green gram), a major pulse crop is prone to damaging diseases caused by Erysiphe polygoni, Cercospora canescens and Rhizoctonia sp. Therefore, the development of multiple resistance is a major breeding objective in green gram. Resistance to powdery mildew has already been developed, however, there are no reports on the development of resistance to Cercospora in green gram. Owing to limitation of conventional screening methods, the improvement for multiple disease resistance is inadequate, in this crop. It needs an efficient and quick selection method, for screening the plant population at an early stage. It is well established that the resistant interaction, in plants, involves accumulation of antibiotic compound phytoalexin (Genestein in Vigna radiata) and induction of enzymes such as β-1,3 gulcanase and Chitinases. These compounds are not only induced by pathogens but also pathogen-derived elicitors. These biochemical compounds can be used as resistance indicative biochemical markers for screening the natural or mutagen induced genetic diversity in populations of Vigna radiata in non-destructive manner. It, however, needs a systematic study of plant defense response. This paper deals with the response of resistant and susceptible cultivars of vigna radiata to Cercospora elicitor and development of non-destructive selection method for disease resistance. (author)

  8. TRATAMENTO DE SEMENTES DE ARROZ, TRIGO, FEIJÃO E SOJA COM UM PRODUTO FORMULADO À BASE DE CÉLULAS E DE METABÓLITOS DE Bacillus subtilis

    OpenAIRE

    LAZZARETTI E; W. Bettiol

    1997-01-01

    Um produto constituido de pó-molhável (PBBS) formulado à base de células (60g) e de metabólitos (60g) de Bacillus subtilis, argila (480g), espalhante (7,92g) e água (2400ml), moído e seco, mostrou-se semelhante aos tratamentos com fungicidas recomendados para o controle de Rhizoctonia solani, Aspergillus sp. e Sclerotinia sclerotiorum em sementes de feijão; Pyricularia oryzae e Rhinchosporium sativum em sementes de arroz; e Cercospora kikuchii, Phomopsis phaseoli e Fusarium spp. em sementes d...

  9. Occurrence of broad bean (Vicia faba L.) diseases in Olsztyn-Elbąg and Bydgoszcz Provinces

    OpenAIRE

    Stanisław Sadowski

    2013-01-01

    During the years 1981-1985, there were conducted studies of the healthiness of broad bean,'Nadwiślański' variety, cultivated in different soil and climate conditions of the two regions: i.e. Bydgoszcz - comparatively warmer and drier, and Olsztyn-Elbląg - colder and moister. It was found that the main reason for a premature broad bean leaves dry in up in the Olsztyn-Elbląg Region was caused by the fungi Cercospora and Botrytis, and in the Bydgoszcz Region - the root rot which occurs here to a...

  10. Synthesis and Biological Activities of Novel Anthranilic Diamides Analogues Containing Benzo[b]thiophene

    Institute of Scientific and Technical Information of China (English)

    ZHANG Ji-feng; LIU Chen; LIU Peng-fei; YAN Tao; WANG Bao-lei; XIONG Li-xia; LI Zheng-ming

    2013-01-01

    A series of novel anthranilic diamides analogues containing benzo[b]thiophenyl ring was designed and synthesized.Their structures were characterized by melting points,1H nuclear magnetic resonance(1H NMR) and high-resolution mass spectrometry(HRMS).The bioassay tests indicate that their insecticidal activities were weak to moderate.Antibacterial tests indicate that some of the compounds showed favourable activity in vitro against Physalospora piricola,Alternaria solani,Cercospora arachidicola,Gibberella sanbinetti and Phytophthora infestans at a dosage of 50 mg/L.

  11. Characterization of a new antifungal non-specific lipid transfer protein (nsLTP) from sugar beet leaves

    DEFF Research Database (Denmark)

    Kristensen, A K; Brunstedt, J; Madsen, M T;

    2000-01-01

    cysteines at conserved positions, the protein can be classified as a member of the plant family of non-specific lipid transfer proteins (nsLTPs). The protein is 47% identical to IWF1, an antifungal nsLTP previously isolated from leaves of sugar beet. A potential site for N-linked glycosylation present in...... sequence of 26 amino acid residues. The protein shows a strong in vitro antifungal activity against Cercospora beticola (causal agent of leaf spot disease in sugar beet) and inhibits fungal growth at concentrations below 10 µg ml(-1)....

  12. UFUS-Imperial: nova cultivar de soja para o Estado de Mato Grosso UFUS-Imperial: new soybean cultivar for the State of Mato Grosso, Brazil

    OpenAIRE

    Osvaldo Toshiyuki Hamawaki; Analy Castilho Polizel; Fernando Cézar Juliatti; Raphael Lemes Hamawaki; Patrícia Brunetta

    2007-01-01

    A fim de ampliar as opções ao cultivo da soja, a Universidade Federal de Uberlândia lançou a cultivar UFUS-Imperial, proveniente do cruzamento entre (Msoy 8411xMsoy 8914) x (Emgopa 313xTucano). A cultivar apresentou resistência ao acamamento, à deiscência da vagem e aos patógenos: Fusarium solani, Cercospora sojina, Peronospora manshurica, Xanthomonas campestris pv. glycines e Diaporthe phaseolorum f.sp. meridionalis, e resistência parcial a Septoria glycines, Erysiphe diffusa e Phakopsora pa...

  13. PARAZITNA MIKOPOPULACIJA ZRNA SOJE

    OpenAIRE

    Ćosić, Jasenka; Vrandečić, Karolina; Jurković, Draženka; Ereš, Ivan; Poštić, Jelena

    2008-01-01

    Pojava bolesti na soji može utjecati na kvalitetu i količinu uroda. Različite vrste saprofitnih i parazitnih gljiva mogu se izolirati sa stabljika, mahuna i sjemena soje. Cilj ovih istraživanja bio je ocijeniti pojavu značajnijih bolesti na naturalnom zrnu soje tijekom 4 godine (2004.-2007.), a provedeno je na lokalitetu Sopot-Vinkovci i obuhvaćalo je 9 sorata soje. Identificirani su uzročnici bolesti iz rodova: Peronospora, Sclerotinia, Cercospora, Fusarium i Diaporthe/Phomopsis. Najčešće iz...

  14. Occurrence of fungal diseases on angelica (Archangelica officinalis Hoffm. in the region of Małopolska

    Directory of Open Access Journals (Sweden)

    Stanisław Mazur

    2012-12-01

    Full Text Available The field experiment on angelica (Archangelica officinalis Hoffm. was carried out in the years 2001-2002 at the plantations situated in Małopolska (Mydlniki near Kraków and Królówka near Bochnia. The results of two years' investigations pointed out, that angelica plants during vegetation were infested by many pathogens. The most often noticed on the plants were angelica rust (Puccinia anglicae, powdery mildew (Erysiphe umbelliferarum and Cercospora leaf spot (Cercospora sp.. Moreover, many various types of disease symptoms, which were described and made a diagnosis. There were mainly spots on leaves and steams, from which there were isolated most often fungi from Alternaria genus. The investigations on the salubrity of the seed material (angelica fruits coming from different sources showed that fungi from genus Alternaria were likely to transfer by seeds and caused disease symptoms on plants during vegetation. Fungi from genus Alternaria were most often isolated from angelica fruits. Saprotrophic fungi (Epicoccum purpurascens, Penicillium spp., Mucor hiemalis were also often isolated from angelica fruits. Disinfection on fruits reduced number of isolated fungi and bacteria.

  15. Soybean Toxin (SBTX) Impairs Fungal Growth by Interfering with Molecular Transport, Carbohydrate/Amino Acid Metabolism and Drug/Stress Responses

    Science.gov (United States)

    Morais, Janne K. S.; Bader, Oliver; Weig, Michael; Oliveira, Jose Tadeu A.; Arantes, Mariana R.; Gomes, Valdirene M.; Da Cunha, Maura; Oliveira, Hermogenes D.; Sousa, Daniele O. B.; Lourencao, Andre L.; Vasconcelos, Ilka M.

    2013-01-01

    Soybean toxin (SBTX) is an antifungal protein from soybeans with broad inhibitory activity against the growth and filamentation of many fungi, including human and plant pathogenic species such as Candida albicans, Candida parapsilosis, Aspergillus niger, Penicillium herquei, Cercospora sojina and Cercospora kikuchii. Understanding the mechanism by which SBTX acts on fungi and yeasts may contribute to the design of novel antifungal drugs and/or the development of transgenic plants resistant to pathogens. To this end, the polymorphic yeast C. albicans was chosen as a model organism and changes in the gene expression profile of strain SC5314 upon exposure to SBTX were examined. Genes that were differentially regulated in the presence of SBTX were involved in glucose transport and starvation-associated stress responses as well as in the control of both the induction and repression of C. albicans hyphal formation. Transmission electron microscopy showed that C. albicans cells exposed to SBTX displayed severe signs of starvation and were heavily granulated. Our data were indicative of C. albicans cell starvation despite sufficient nutrient availability in the medium; therefore, it can be speculated that SBTX blocks nutrient uptake systems. Because neither the starvation signal nor the alkaline response pathway lead to the induction of hyphae, we hypothesise that conflicting signals are transmitted to the complex regulatory network controlling morphogenesis, eventually preventing the filamentation signal from reaching a significant threshold. PMID:23894655

  16. Pathogenicity of seed-borne and seedling fungi of groundnut cv. Tatu/ Patogenicidade de fungos associados às sementes e plântulas de amendoim cv. Tatu

    Directory of Open Access Journals (Sweden)

    Débora Cristina Santiago

    2005-06-01

    Full Text Available Seeds and seedlings of groundnut (Arachis hypogaea cv. Tatu were investigated under laboratory and green house conditions to detect and identify any seed-borne pathogenic fungi present, besides determining the pathogenicity and the seed damages. The fungi detected were Aspergillus niger, Fusarium oxysporum, Cercospora arachidicola, Rhizopus sp., Penicillium sp., Cladosporium sp. and Phoma sp.. The most frequently isolated fungi were Aspergillus niger and Penicillium sp.. These fungi were transmitted by seeds and caused damping-off before and after seedling emergence.Sementes e plântulas de amendoim (Arachis hypogaea cv. Tatu foram avaliadas em condições de laboratório e casa de vegetação com o objetivo de detectar e identificar a presença de fungos patogênicos associados às sementes, além de determinar a patogenicidade e os danos por eles causados. Os fungos detectados foram Aspergillus niger, Fusarium oxysporum, Cercospora arachidicola, Rhizopus sp., Penicillium sp., Cladosporium sp. e Phoma sp.. Os fungos mais freqüentes foram A. niger e Penicillium sp.. Estes fungos foram transmitidos através das sementes e causaram tombamento de pré e pósemergência nas plântulas.

  17. UFUS-Imperial: nova cultivar de soja para o Estado de Mato Grosso UFUS-Imperial: new soybean cultivar for the State of Mato Grosso, Brazil

    Directory of Open Access Journals (Sweden)

    Osvaldo Toshiyuki Hamawaki

    2007-01-01

    Full Text Available A fim de ampliar as opções ao cultivo da soja, a Universidade Federal de Uberlândia lançou a cultivar UFUS-Imperial, proveniente do cruzamento entre (Msoy 8411xMsoy 8914 x (Emgopa 313xTucano. A cultivar apresentou resistência ao acamamento, à deiscência da vagem e aos patógenos: Fusarium solani, Cercospora sojina, Peronospora manshurica, Xanthomonas campestris pv. glycines e Diaporthe phaseolorum f.sp. meridionalis, e resistência parcial a Septoria glycines, Erysiphe diffusa e Phakopsora pachyrhizi. O rendimento dessa cultivar, em ensaios regionais, foi 45% superior ao da testemunha Msoy 6101, e é indicada para o Estado de Mato Grosso.With the purpose of offering new options for soybean production, the Universidade Federal de Uberlândia released the cultivar UFUS-Imperial, originated from the crosses between (Msoy 8411xMsoy 8914 x (Emgopa 313xTucano. It showed resistance to lodging, to pod shattering, and to the pathogens Fusarium solani, Cercospora sojina, Peronospora manshurica, Xanthomonas campestris pv. glycines and Diaporthe phaseolorum f.sp. meridionalis, and partial resistance to Septoria glycines, Erysiphe diffusa, and Phakopsora pachyrhizi. Its average yield from uniform trials was 45% higher than that of the control Msoy 6101, and it is indicated for cultivation in the State of Mato Grosso.

  18. Impact of Poultry Manure on Growth Behaviour, Black Sigatoka Disease Response and Yield Attributes of Two Plantain (Musa spp. AAB Genotypes

    Directory of Open Access Journals (Sweden)

    Aba, SC.

    2011-01-01

    Full Text Available La baisse de rendement en culture de plantain est un phénomène fréquent après quelques cycles de production; elle est due à de faibles niveaux de matière organique du sol et à une sensibilité accrue à des stress biotiques. L'utilisation de variétés améliorées est une option bon marché et respectueuse de l'environnement pour lutter contre les maladies des plantes, mais le maintien du rendement d'un nouveau cultivar en bananeraie nécessite une bonne gestion de la fertilité des sols qui pourrait être obtenue par une utilisation judicieuse de fumier de volaille. Les effets des trois doses [(0, 10 et 20 t.ha-1] de fumier de volaille décomposé sur la croissance, la réponse à la cercosporiose noire et le rendement de PITA 24' (un hybride de plantain et de son grand-parent maternel 'Mbi-Egome' (une banane plantain locale ont été évalués à Onne (Nigeria dans une station à fortes précipitations de l'IITA. L'expérience a été menée selon un dispositif split plot en blocs aléatoires complets. Les résultats ont montré que les deux clones étudiés avaient des variables de croissance similaires, sauf pour la surface foliaire et la teneur en chlorophylle. Malgré la sensibilité du génotype hybride à la cercosporiose foliaire d'origine atmosphérique, les données sur les composantes du rendement ont montré que 'PITA 24' produisait significativement plus de mains et de doigts par régime, un meilleur rendement du régime et un meilleur rendement végétatif global, toutefois, les deux génotypes ont présenté un schéma similaire pour la répartition de la biomasse. Les plants fumés ont eu un rendement significativement amélioré par rapport aux plants témoins, mais il y a eu un rendement moindre pour la dose de 20 t.ha-1. L'augmentation des doses d'engrais a réduit de plus de 30 jours le temps de récolte; de même, la taille des plants, le drageonnage, la teneur en chlorophylle des feuilles, l'indice des feuilles non

  19. Permanent genetic resources added to Molecular Ecology Resources Database 1 February 2012 - 31 March 2012.

    Science.gov (United States)

    Andris, Malvina; Arias, M C; Barthel, Brandon L; Bluhm, Burton H; Bried, Joël; Canal, D; Chen, X M; Cheng, P; Chiappero, Marina B; Coelho, Manuela M; Collins, Angela B; Dash, M; Davis, Michelle C; Duarte, Margarida; Dubois, Marie-Pierre; Françoso, E; Galmes, M A; Gopal, Keshni; Jarne, Philippe; Kalbe, Martin; Karczmarski, Leszek; Kim, Hun; Martella, Mónica B; McBride, Richard S; Negri, Valeria; Negro, J J; Newell, Annakay D; Piedade, Ana F; Puchulutegui, Cecilia; Raggi, Lorenzo; Samonte, Irene E; Sarasola, J H; See, D R; Seyoum, Seifu; Silva, Mónica C; Solaro, C; Tolley, Krystal A; Tringali, Michael D; Vasemägi, A; Xu, L S; Zanón-Martínez, J I

    2012-07-01

    This article documents the addition of 171 microsatellite marker loci and 27 pairs of single nucleotide polymorphism (SNP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Bombus pauloensis, Cephalorhynchus heavisidii, Cercospora sojina, Harpyhaliaetus coronatus, Hordeum vulgare, Lachnolaimus maximus, Oceanodroma monteiroi, Puccinia striiformis f. sp. tritici, Rhea americana, Salmo salar, Salmo trutta, Schistocephalus solidus, Sousa plumbea and Tursiops aduncus. These loci were cross-tested on the following species: Aquila heliaca, Bulweria bulwerii, Buteo buteo, Buteo swainsoni, Falco rusticolus, Haliaeetus albicilla, Halobaena caerulea, Hieraaetus fasciatus, Oceanodroma castro, Puccinia graminis f. sp. Tritici, Puccinia triticina, Rhea pennata and Schistocephalus pungitii. This article also documents the addition of 27 sequencing primer pairs for Puffinus baroli and Bulweria bulwerii and cross-testing of these loci in Oceanodroma castro, Pelagodroma marina, Pelecanoides georgicus, Pelecanoides urinatrix, Thalassarche chrysostoma and Thalassarche melanophrys. PMID:22642264

  20. Bambara groundnut improvement through mutation breeding in Ghana

    International Nuclear Information System (INIS)

    A study was initiated to improve bambara groundnut productivity using mutation breeding. Two landraces of varieties Tom and Nav were treated with gamma rays ranging from 50 to 400 Gy and with EMS at concentrations from 0.2 to 1.2%. Pooled mean LD50 for gamma irradiation were 178.5 Gy and 0.78% for EMS treatment respectively. Gamma irradiation gave effective dosages for mutation frequency at 150 Gy for Tom and 200 Gy for Nav, while EMS treatment gave 0.6% for both Tom and Nav. Genetic variation of up to four times the control was estimated for both gamma irradiation and EMS treatment compared to the untreated controls. Selected mutants outperformed the untreated control plants in most of the agronomic traits and reaction to diseases like Cercospora leaf spot, anthracnose and viruses. The results indicate that mutation breeding could be used to improve bambara groundnut productivity. (author)

  1. A novel small antifungal peptide from Bacillus strain B-TL2 isolated from tobacco stems.

    Science.gov (United States)

    Zhang, Beibei; Xie, Chengjian; Yang, Xingyong

    2008-03-01

    A novel small antifungal peptide produced by a Bacillus strain B-TL2 isolated from tobacco stems was purified. The purification procedure consisted of ammonium sulfate precipitation, cation exchange chromatography on CM-Sepharose Fast Flow column and reverse-phase HPLC on SOURCE 5RPC column. After the final isolation step, one peptide with antifungal activity, designated as BTL, was obtained. The molecular mass of the purified BTL was determined as 2500 Da and 2237.7 Da by SDS-PAGE and matrix-assisted laser desorption/ionization time of flight mass spectrometry, respectively. The N-amino acid sequence of BTL was determined to be NH(2)-KQQLATEAESAGPIL, which shows relatively low identity to other antimicrobial peptides from bacteria. The peptide exhibited strong inhibitory activity against mycelial growth of Bipolaris maydis, Alternaria brassicae, Aspergillus niger, Cercospora personata. The purified BTL displayed thermostability, almost retaining 100% activity at 100 degrees C for 15 min. PMID:18241956

  2. Influence of He-Ne laser irradiation of soybean seeds on seed mycoflora, growth, nodulation, and resistance to Fusarium solani

    International Nuclear Information System (INIS)

    Laser irradiation of soybean seeds for 3 min caused a clear reduction in the number of seed-borne fungi which became more pronounced as the irradiation time was extended. Pretreatment of the seeds with methylene blue, methyl red and carmine enhanced the effect of laser. Rhizoctonia solani, Alternaria tenuissima, Cercospora kikuchii and Colletotrichum truncatum were completely eliminated when the seeds were pretreated with a dye and irradiated for 10 min. Seed germination was stimulated on exposure of the seed to 1-min irradiation. Chlorophyll a, chlorophyll b and carotenoid content of developed plants differed, depending on the irradiation dose and dye treatment of the seeds. The number and dry mass of nodules were mostly greater (as compared to the corresponding control), when the seeds irradiated for 1 or 3 min were pretreated with methyl red, chlorophenol red, crystal violet and methylene blue. Irradiation of pre-sowing seeds greatly protected soybean stands against F. solani

  3. PARASITE MYCOPOPULATION OF SOYBEAN GRAIN

    Directory of Open Access Journals (Sweden)

    Jasenka Ćosić

    2008-06-01

    Full Text Available Disease appearance on soybean can influence quality and quantity of yield. Different spieces of saprophyte and parasite fungi can be isolated from stems, pods and grain of soybean. The aim of the research was to evaluate the incidence of important disease on natural soybean grain over the period of 4 years (2004-2007 of experiment held on the location Sopot-Vinkovci and included 9 cultivars of soybean. The following plant pathogenic fungi were identified: Peronospora, Sclerotinia, Cercospora, Fusarium and Diaporthe/Phomopsis. The most frequent fungi on soybean grains were: Cladosporium, Alternaria, Penicillium, Aspergillus and Epicoccum. The health condition of the natural soybean grains over the four years period on all cultivars was good.

  4. Synthesis and antifungal activity of 2-hydroxy-4,5-methylenedioxyaryl ketones as analogues of kakuol.

    Science.gov (United States)

    Musso, Loana; Dallavalle, Sabrina; Merlini, Lucio; Farina, Gandolfina

    2010-04-01

    In a study aiming to determine the structural elements essential to the antifungal activity of kakuol, we synthesized a series of 2-hydroxy-4,5-methylenedioxyaryl ketones, and we assayed their in vitro antifungal activity. The most sensitive target organisms to the action of these class of compounds were Phytophthora infestans, Phytium ultimum, Cercospora beticola, Cladosporium cucumerinum, and Rhizoctonia solani. Most of the analogs showed a remarkable in vitro activity, and some of them appeared significantly more effective than the natural product. The biological activity was mainly affected by introducing structural modification on the carbonyl moiety of the natural-product molecule. In particular, compound 5a, bearing a C=C bond conjugated to the C=O group, was found active with a MIC value of 10 microg ml(-1) against Cladosporium cucumerinum. The results suggest that 2-hydroxy-4,5-methylenedioxyaryl ketones can be considered promising candidates in the development of new antifungal compounds. PMID:20397224

  5. Breeding of improved grain legume genotypes for Bangladesh through nuclear and conventional techniques

    International Nuclear Information System (INIS)

    Grain legumes are important in Bangladesh for human diet, as cattle feed, for cropping pattern and soil fertility. But their production has been steadily declining owing to severe competition from cereals. Development of improved genotypes is problematic because of the lack of adequate genetic variability in these legumes. Mutation breeding programmes on chickpea (Cicer arietinum L.), mungbean (Vigna radiata (L.) Wilczek) and blackgram (Vigna mungo (L.) Hepper) were therefore attempted in order to create adequate variability and later selection of mutans. Adaptability studies with exotic genotypes were also carried out. In chick-pea, one high yielding, high protein variety named Hyprosola, with 20% higher yield and 4% increased protein per unit seed weight, has been released from the gamma irradiated material. Three mutants and one exotic germplasm with higher yield and desirable agronomic characters are in advanced stages of development. Sodium azide treatment has resulted in isolation and development of genotypes with an increased number of pods/plant and higher seed yield. Gamma irradiation of mungbean has yielded mutants with 15-17% higher yield and resistance/tolerance to mungbean yellow mosaic virus (MYMV) and Cercospora leaf spot diseases. These are ready for release. Mutants with erect growth habit and synchrony in pod maturity were selected from the sodium azide treated population. Adaptation studies of the exotic and local germplasm have resulted in identification of genotypes suitable for growing during various seasons of the year. It has been possible to isolate and develop one blackgram mutant through irradiation with 15% higher seed yield and tolerance/resistance to Cercospora leaf spot disease and yellow mosaic virus (YMV). 41 refs, 1 fig., 17 tabs

  6. Environmental Factors in the Growth of Jatropha at Potorono Village, Yogyakarta

    Directory of Open Access Journals (Sweden)

    Mohammad Nurcholis

    2015-01-01

    Full Text Available Jatropha curcas is a perennial crop that has been known by Indonesian people for more than seven decades as a plant that produces renewable biofuel. In the present decade, plants producing biofuel are expected to be developed to overcome the lowering nonrenewable fuel reserves. There is a myth that jatropha can grow well on marginal lands and draught condition, perform well on non-fertile soils, no need for agronomic management and is resistant to plant pests and diseases. This study was conducted to identify the environmental factors that influenced the growth of jatropha on the marginal land at Potorono village, Yogyakarta Province, Indonesia. Jatropha has been planted by local people at the village road sides and on the marginal land field at the local governmental land in this village. They grew jatropha on these areas with the purpose of preventing competition of area utilization with food crops. The results showed that the growth of jatropha was restricted by low content of organic matter, plant nutrition and poor soil drainage. Applications of manure and macro nutrients (N, P and K to this crop were able to increase crop performance. The number of shoots, flowers and fruit bunches increased by manure and nutrients treatments. Field observation showed that there were several plant pests, such as Aspidiotus sp., Paracoccus marginatus, Poliphagotarsonemus latus, Selenothrips rubrocinctus, Chrysochoris javanus, Valanga nigricornis, Chloracris prasina, and Helicoverpa armigera that attacked plant leaves and fruits. There were plant leaf necrotic symptoms that caused by plant pathogens were also observed. The diseases are bacterial leaf spot (Xanthomonas ricinicola, cercospora leaf spot (Cercospora ricinella and rust (Phakopsora jatrophicola. Thus, jatropha is like any other plants that need a good agro-ecological condition to grow well and produce high yield.

  7. Severidade da mela da soja causada por Rhizoctonia solani AG-1 IA em função de doses de potássio Severity of hte foliar blight of the soylean caused by Rhizoctonia solani AG-1 IA infunction of doses of potassium

    Directory of Open Access Journals (Sweden)

    Marco Antonio Basseto

    2007-03-01

    Full Text Available O fungo Rhizoctonia solani pertencente ao grupo de anastomose 1 IA (AG-1 IA é um dos patógenos mais importantes afetando a cultura da soja no Brasil. Este fungo causa queima da folha e/ou mela em soja, para a qual medidas de manejo cultural são consideradas alternativas importantes para controle antes do estabelecimento da doença. Há evidências de que a adubação potássica diminui substancialmente a severidade dos sintomas de várias doenças da soja como a queima foliar (Cercospora kikuchii, a seca da haste e da vagem (Phomopsis phaseoli var. sojae e o cancro da haste (Diaporthe phaseolorum f. sp. meridionalis. Apesar das evidências do efeito do potássio no controle de várias doenças da soja, não há informação na literatura sobre o efeito desse nutriente no controle da mela. A hipótese testada foi que a mela da soja pode ser controlada através de incrementos na adubação potássica. De maneira geral, concluiu-se que, sob condições de casa de vegetação, o incremento de K no solo não resultou no controle da mela da soja. É necessário, entretanto, confirmar esta observação conduzindo-se experimentos sob condições de campo, podendo-se incluir a avaliação do efeito da doença sob aspectos da produção.The fungus Rhizoctonia solani, belonging to anastomosis group 1IA (AG-1 IA is one of the most important pathogens affecting soybean in Brazil. This fungus causes aerial or foliar blight of soybean, and cultural measures are thought as important choices for the control before the establishment of the disease. Based on evidences that potassium amendments can substantially reduce the severity of several soybean diseases such as Cercospora leaf blight (Cercospora kikuchii, pod and stem blight (Phomopsis phaseoli var. sojae and stem canker (Diaporthe phaseolorum f. sp. meridionalis. Despite all evidence, there is no information in the literature about the effect of potassium controlling the soybean foliar blight. The

  8. TRATAMENTO DE SEMENTES DE ARROZ, TRIGO, FEIJÃO E SOJA COM UM PRODUTO FORMULADO À BASE DE CÉLULAS E DE METABÓLITOS DE Bacillus subtilis

    Directory of Open Access Journals (Sweden)

    E LAZZARETTI

    1997-01-01

    Full Text Available Um produto constituido de pó-molhável (PBBS formulado à base de células (60g e de metabólitos (60g de Bacillus subtilis, argila (480g, espalhante (7,92g e água (2400ml, moído e seco, mostrou-se semelhante aos tratamentos com fungicidas recomendados para o controle de Rhizoctonia solani, Aspergillus sp. e Sclerotinia sclerotiorum em sementes de feijão; Pyricularia oryzae e Rhinchosporium sativum em sementes de arroz; e Cercospora kikuchii, Phomopsis phaseoli e Fusarium spp. em sementes de soja. Para Dreschlera oryzae em arroz e Bipolaris sorokiniana, P. oryzae e Alternaria tenuis em sementes de trigo, o tratamento com o PBBS, embora não tenha se igualado ao tratamento com o fungicida padrão, diferiu estatisticamente do tratamento testemunha. A nodulação das raízes de feijão e soja por bactérias simbióticas, fixadoras de nitrogênio, não foi influenciada quando o PBBS foi aplicado simultaneamente ao inoculante contendo Rhyzobium sp. e Bradyrhizobium japonicum, respectivamente. O PBBS também não afetou a emergência das plântulas das culturas testadas.A biological fungicide product containing B. subtilis cells (60 g and metabolites (60g was transformed into a wettable powder formulated with, clay (480 g, surfactant (7,92 g and water (2400 ml.The product showed to be similar to the conventional fungicides used in the control of Rhizoctonia solani, Aspergillus sp. and Sclerotinia sclerotiorum in bean seeds. The control of Pyricularia oryzae and Rhinchosporium sativum in rice seeds and Cercospora kikuchii, Phomopsis phaseoli and Fusarium spp. in soybean seeds was also as efficient as the comercial fungicides. For Dreschlera oryzae in rice seeds and Bipolaris sorokiniana, P. oryzae and Alternaria tenuis in wheat seeds, the treatment with the product, althought not as efficient as the chemical treatment, was statistically distinct from the control. The root nodulation of bean and soybean by nitrogen fixing bacteria, was not affected

  9. Ocorrência de doenças em plantas ornamentais tropicais no Estado de Pernambuco Occurrence of diseases in ornamental tropical flowers in the State of Pernambuco

    Directory of Open Access Journals (Sweden)

    Severina R. O. Lins

    2004-06-01

    Full Text Available As condições de cultivo das plantas ornamentais tropicais, relacionadas aos fatores precipitação, umidade, temperatura e densidade de plantio, favorecem a ocorrência de doenças que limitam a produção e reduzem a qualidade das flores. Destacaram-se as doenças causadas por fungos e nematóides, sendo assinaladas a antracnose (Colletotrichum gloeosporioides em Heliconia spp., Etlingera elatior, Tapeinochilos ananassae, causando lesões em folhas e inflorescências; manchas foliares (Bipolaris spp., Cercospora sp., Curvularia lunata, Glomerella cingulata, Guignardia sp. e Deigthoniella torulosa em Heliconia spp., Calathea burle marx e Musa coccinea; podridão de rizomas e raízes (Rhizoctonia solani e Fusarium oxysporum f. sp. cubense em E. elatior e Heliconia chartacea cv. Sex Pink. As fitonematoses, causadas por espécies dos gêneros Meloidogyne, Radopholus e Helicotylenchus, constituem um dos principais problemas sanitários em ornamentais tropicais em Pernambuco, ocorrendo comumente em Alpinia purpurata, E. elatior, Zingiber espectabiles, Heliconia spp. e Musa spp. A espécie A. purpurata foi a mais suscetível a M. incognita. Em função dos trabalhos de erradicação pelos produtores, a murcha bacteriana (Ralstonia solanacearum raça 2 foi assinalada com baixa incidência nas áreas de cultivo de flores tropicais.The climatic conditions in which tropical flowers are grown in Northeast Brazil, related to rainfall, humidity, temperature and cropping density factors, favor disease occurrence that limit and reduce the quality and yield of flowers. In this survey several diseases caused by fungi and nematodes were observed. Among them were the anthracnose (Colletotrichum gloeosporioides on Heliconia spp, Etlingera elatior, Tapeinochilos ananassae, causing leaf and inflorescence lesions; leaf spots (Bipolaris spp., Cercospora sp., Curvularia lunata, Glomerella cingulata, Guignardia sp and Deighthoniela torulosa on Heliconia app, Calathea

  10. Resistance to gray leaf spot of maize: genetic architecture and mechanisms elucidated through nested association mapping and near-isogenic line analysis.

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    Jacqueline M Benson

    2015-03-01

    Full Text Available Gray leaf spot (GLS, caused by Cercospora zeae-maydis and Cercospora zeina, is one of the most important diseases of maize worldwide. The pathogen has a necrotrophic lifestyle and no major genes are known for GLS. Quantitative resistance, although poorly understood, is important for GLS management. We used genetic mapping to refine understanding of the genetic architecture of GLS resistance and to develop hypotheses regarding the mechanisms underlying quantitative disease resistance (QDR loci. Nested association mapping (NAM was used to identify 16 quantitative trait loci (QTL for QDR to GLS, including seven novel QTL, each of which demonstrated allelic series with significant effects above and below the magnitude of the B73 reference allele. Alleles at three QTL, qGLS1.04, qGLS2.09, and qGLS4.05, conferred disease reductions of greater than 10%. Interactions between loci were detected for three pairs of loci, including an interaction between iqGLS4.05 and qGLS7.03. Near-isogenic lines (NILs were developed to confirm and fine-map three of the 16 QTL, and to develop hypotheses regarding mechanisms of resistance. qGLS1.04 was fine-mapped from an interval of 27.0 Mb to two intervals of 6.5 Mb and 5.2 Mb, consistent with the hypothesis that multiple genes underlie highly significant QTL identified by NAM. qGLS2.09, which was also associated with maturity (days to anthesis and with resistance to southern leaf blight, was narrowed to a 4-Mb interval. The distance between major leaf veins was strongly associated with resistance to GLS at qGLS4.05. NILs for qGLS1.04 were treated with the C. zeae-maydis toxin cercosporin to test the role of host-specific toxin in QDR. Cercosporin exposure increased expression of a putative flavin-monooxygenase (FMO gene, a candidate detoxification-related gene underlying qGLS1.04. This integrated approach to confirming QTL and characterizing the potential underlying mechanisms advances the understanding of QDR and will

  11. Ocorrência de fungos em sementes de soja produzidas sob calagem e adubação potássica residuais The residual effect of liming and potassium fertilization on the occurrence of fungi in soybean seeds

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    H.A.A. Mascarenhas

    1995-12-01

    Full Text Available Estudou-se o efeito residual da adubação potássica e da calagem sobre a ocorrência de fungos em sementes de soja. O experimento foi instalado com a cultivar IAC-17, no ano agrícola de 1991/92, aplicando doses a lanço de O, 3,5 e 7 t/ha de calcário dolomítico e de 0, 150, 300, 450 e 600 kg/ha de K2O. As sementes colhidas no terceiro ano de cultivo, (1993/94 foram submetidas ao teste de sanidade, pelo método do papel de filtro. Observou-se que a calagem reduziu significativamente a incidência de Phomopsis sp., favorecendo, no entanto, a presença de Aspergillus sp. e de Fusarium sp.. Embora não tenham sido detectadas diferenças significativas, nos tratamentos com calagem, verificou-se redução da incidência de Cercospora kikuchii, Colltotrichum dematium var. truncata e Peronospora manshurica. Apenas a incidência de Phomopsis sp. diminuiu significativamente devido à adubação potássica, sendo menor quando se utilizou a dose de 450 kg/ha de K2O.To study the residual effect of liming and potassium fertilizer on the occurrence of fungi in soybean seeds, an experiment was installed during the 1991/92 growing season, using 0, 3.5 and 7.0 t/ha of dolomitic lime and 0, 150, 300, 450 and 600 kg/ha of K2O in the form of potassium cloride. In 1993/94 (the third year, the harvested seeds were submitted to a seed health test (blotter method. The results showed that liming reduced significantly the incidence of Phomopsis sp. and increased the presence of Aspergillus sp. and Fusarium sp.. Although no significant differences were observed with liming, there was a reduction in the incidence of Cercospora kikuchii, Colletotrichum dematium var. truncala and Peronospora manshurica. Among the fungi found in the seeds, only Phomopsis sp. was significantly reduced by potassium fertilization, the least incidence being observed at the rate of 450 kg/ha.

  12. Survival of pathogens on soybean debris under no-tillage and conventional tillage systems Sobrevivência de patógenos em restos de cultura de soja mantidos em sistema de semeadura direta e convencional

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    Álvaro Manuel Rodrigues Almeida

    2001-10-01

    Full Text Available A study was conducted in the subtropical area of Southern Brazil to determine the survival of pathogens in soybean residues under conventional and no-tillage cultivation systems from March to September of 1998 and 1999. The pathogens most frequently isolated were Colletotrichum truncatum, Phomopsis spp., Cercospora kikuchii, Fusarium spp., Macrophomina phaseolina, and Rhizoctonia solani. Other fungi isolated were Myrothecium roridum, Penicillium sp., Chaetomium sp., Epicoccum sp., Corynespora cassiicola and Trichoderma sp. The percent of survival of each pathogen varied according to the month and the year. Survival of C. truncatum, Phomopsis spp. and C. kikuchii were significantly reduced (pAvaliou-se a sobrevivência de patógenos em restos de soja, em sistema de semeadura direta e convencional, entre março e setembro de 1998 e 1999, em Londrina, PR. Os patógenos mais freqüentemente isolados foram Colletotrichum truncatum, Phomopsis spp., Cercospora kikuchii, Fusarium spp., Macrophomina phaseolina e Rhizoctonia solani. Outros fungos isolados foram Myrothecium roridum, Penicillium sp., Chaetomium sp., Epicoccum sp., Corynespora cassiicola e Trichoderma sp. A porcentagem de sobrevivência variou com o mês e o ano. A sobrevivência de C. truncatum, Phomopsis spp. e C. kikuchii foi significativamente reduzida (P<0,05 entre a primeira e última avaliação nos resíduos mantidos sobre ou sob o solo. M. phaseolina e Fusarium spp. não foram afetados, ou foram favorecidos pelo enterro dos resíduos. A freqüência de isolamento de Fusarium spp. aumentou em resíduos enterrados no solo. A perda de biomassa mostrou redução de 44,4% no sistema convencional e 34,9% no sistema de semeadura direta, em 1998, quando a distribuição de chuvas foi mais regular. Em 1999, a redução foi de 48,2% e 39,0% para os sistemas convencional e de semeadura direta, respectivamente.

  13. A disease resistant and high yielding blackgram variety, BINAMASH-1, derived through induced mutation

    International Nuclear Information System (INIS)

    Blackgram (Vigna mungo L.), the second most important pulse crop of Bangladesh, is low yielding as well as susceptible to Cercospora leaf spote and yellow mosai virus. Air dried seeds of the local cultivar, BINA accession No. B-10, were irradiated with a 500 Gy dose of 60Co gamam rays and 1382 M1 plants were chosen for collecting the M2 seeds. Macromutants were selected from the M2 population and plant progeny rows were grown during the M3 generation for identifying true breding lines. The true breeding lines were further tested in the M4 generation. Observation trials, preliminary yield trials, yield trials, advanced yield trials, zonal yield trials and on-farm trials followed in the succeeding generations. In all these trials, one mutant (M-25) showed its superiority with respect to seed yield. On average, it produced a 31% higher yield than its parental cultivar, B-10. The mutant also showed a moderately resistant to resistant reaction against Cercorspora leaf spot and yellow mosaic virus diseases. The mutant M-25 was released as a national variety in 1994 and named BINAMASH-1. 1 ref., 3 tabs

  14. Inventory of Green Spaces and Woody Plants in the Urban Landscape in Ariogala

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    Lina Straigytė

    2012-12-01

    Full Text Available Background and Purpose: Regulation of urban greenery design, management and protection was approved in 2008 in Lithuania after the Green Space Law was passed, allowing protection of public green spaces and woody plants. Protection of these resources first requires an inventory, and we have created a digital database that will help in management of urban green spaces. Material and Methods: An inventory of green spaces and woody plants was conducted in the public urban territory of Ariogala, using GIS technology. A digital cartographic database was created using ArcGis 9.1 software. Results and Conclusion: Most of the woody plants in the survey area are deciduous trees, and the survey results highlighted the major green space management problems. Often, planted trees grow under power lines, and their crowns touch the power cables. Near blocks of flats, trees are often in the wrong place-planted too close to buildings, trees shade windows and their roots heave pavers and penetrate building foundations. According to the inventory, street trees sustain the most damage, most commonly showing injuries on their trunks and roots. Leaves of Aesculus hipocastanum L. show massive damage from Cameraria ohridella Deschka & Dimić, and Tilia cordata Mill. are damaged by Cercospora microsora Sacc. T. cordata is a favourite city tree, but is susceptible to infestation and when damaged appears unsightly, ending its vegetation period very early. The inventory of green spaces also showed that there are sufficient public parks.

  15. Genetic improvement of sesame by induced mutations

    International Nuclear Information System (INIS)

    Full text: This project started in 1983 with assistance by IAEA at the Agricultural Department of Zulia University and with co-operation of FONALI. The main objective was the development of mutants with early ripening, between 85 and 95 days, which are drought and disease resistant. During experimentation in Portuguesa and Oriente, with materials produced under this project researchers identified about 20 mutants that show promise in terms of high yield, earliness and disease resistance. Among them are two mutants of the Piritu variety, P-10-7412 (early), and P-10-7412 (medium); four mutants of Criollo Falcon, CF 6-N3H494, CF 53-8874, CF 35-9306 and CF 25-9382, and a Venezuela 44 mutant, III-8408. They exhibit early flowering at 35-45 days, are resistant to the problematic leaf diseases (Cylindrosporium and Cercospora, are resistant to Macrophomina, are drought tolerant and possibly also virus resistant. These new potential varieties coincide in ripening time, have a good appearance, and show little or no branching, good root development and a good average yield of at least 1000 kg/ha, with a potential of 1500-2000 kg/ha. (author)

  16. Present status of the development of mycoherbicides against water hyacinth: successes and challenges. A review

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    Jijakli, MH.

    2012-01-01

    Full Text Available Recent trends in the implementation of bioherbicide use in the control of water hyacinth (Eichhornia crassipes [Martius] Solms Laubach have depended primarily on several strategies. The use of bioherbicides has been stimulated as part of the search for alternatives to chemical control, as the use of these more environmentally-friendly formulations minimizes hazards resulting from herbicide residue to both human and animal health, and to the ecology. In addition, one of the major strategies in the concept of biological control is the attempt to incorporate biological weed control methods as a component of integrated weed management, in order to achieve satisfactory results while reducing herbicide application to a minimum. Several fungal pathogens with mycoherbicide potential (Sclerotinia sclerotiorum in Hyakillä and Cercospora rodmanii, named ABG-5003 have been discovered on diseased water hyacinth plants, but none has become commercially available in the market. Biological, technological, and commercial constraints have hindered progress in this area. Many of these constraints are being addressed, but there is a critical need to better understand the biochemical and physiological data regarding the pathogenesis of these new bioherbicides. Oil emulsions are recognized as a way to increase both efficiency of application and efficacy of biocontrol agents.

  17. Development of three groundnut varieties with improved quantitative and qualitative traits through induced mutation

    International Nuclear Information System (INIS)

    With a view to develop high yielding, bold seeded and disease resistant varieties of groundnut, 200 dry seeds of an established mutant, Mut-6, was irradiated with 200 Gy gamma rays. All the M1 plants were harvested and kept separately. In M2-M4 generations, selection and evaluation were made following high mean and high/low variances compared to the check cultivar Dhaka-1 (the parent of Mut-6). In M4 generation, 16 true breeding lines were obtained. Through preliminary, advance, zonal and farmers' field trials during 1997-1999, three mutant lines M6/20/42-M(2), M6/20/44-3 and M6/20/62-4, were identified to be dwarf, high yielding, bold podded and seeded and moderately resistant to cercospora leafspot, rust and collar rot diseases, compared to the check variety, Dhaka-1. Moreover, these mutants have higher oil contents and higher/similar protein contents. The National Seed Board has registered these mutant families in 2000 as BINAchinabadam-1, BINAchinabadam- 2 and BINAchinabadam-3, respectively, for commercial cultivation by the farmers. (author)

  18. Detection of Disease Symptoms on Hyperspectral 3d Plant Models

    Science.gov (United States)

    Roscher, Ribana; Behmann, Jan; Mahlein, Anne-Katrin; Dupuis, Jan; Kuhlmann, Heiner; Plümer, Lutz

    2016-06-01

    We analyze the benefit of combining hyperspectral images information with 3D geometry information for the detection of Cercospora leaf spot disease symptoms on sugar beet plants. Besides commonly used one-class Support Vector Machines, we utilize an unsupervised sparse representation-based approach with group sparsity prior. Geometry information is incorporated by representing each sample of interest with an inclination-sorted dictionary, which can be seen as an 1D topographic dictionary. We compare this approach with a sparse representation based approach without geometry information and One-Class Support Vector Machines. One-Class Support Vector Machines are applied to hyperspectral data without geometry information as well as to hyperspectral images with additional pixelwise inclination information. Our results show a gain in accuracy when using geometry information beside spectral information regardless of the used approach. However, both methods have different demands on the data when applied to new test data sets. One-Class Support Vector Machines require full inclination information on test and training data whereas the topographic dictionary approach only need spectral information for reconstruction of test data once the dictionary is build by spectra with inclination.

  19. Evaluation of native and collected Germplasm for earliness Seed traits and resistance to rust, CMV and leaf spot in Cowpea [Vigna unguiculata (L. Walp].

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    K Sanjeev. Deshpand, B.R.Patil, P. M. Salimath , J.M. Nidagundi and S. Karthigeyan

    2010-07-01

    Full Text Available In the present study, 225 germplasm collections of cowpea including local types were evaluated in augmented design alongwith C152 (check for high test weight, desirable seed and pod features, earliness and resistance to Cowpea rust (Uromycesvignae, Cowpea Mosaic Virus (CMV and Cercospora leaf spot for two years ( kharif 2008 and kharif 2009 at Dharwad. Theresults of the investigation revealed that fifteen of the accessions showed bold seeds (test weight > 10g. Accession numberIC202932 and IC247435 showed extra bold seeds (test weight >14g and also bold pods. About 15 germplasm accessions werefound to be highly resistant to rust; 10 accessions displayed HR reaction to CMV and about 5 accessions showed highlyresistant reaction against leafspot. With respect to Multiple Disease Resistance, accession numbers IC201095, IC257406,IC257435 showed highly resistant reaction against leaf spot and CMV and accessions IC257410 and IC214753 showedresistance against rust and leaf spot. Some of the accessions identified against earliness are IC201087, IC201099,IC202707and IC202709 (68-75 days maturity. Such of the superior germplasm lines identified for earliness, multiple diseaseresistance, bold seed (100 seed weight > 12g and desirable seed features would be further useful in Cowpea breeding programas parents.

  20. Status of disease and insect in alfalfa and their controls in Linxia Regions, Gansu province%临夏地区紫花苜蓿病虫害发生现状调查及其防治对策

    Institute of Scientific and Technical Information of China (English)

    鲁鸿佩; 孙爱华

    2008-01-01

    经过3年多的田间调查和实验室鉴定,初步查清了临夏地区紫花苜蓿Medicago sativa病虫害发生的种类、分布、特点和危害程度.临夏地区苜蓿病害主要有褐斑病Pseudopeziza medicaginis、锈病Uromyecsstriatus、白粉病Leueillula leguminosarum、霜霉病Peronos pora estioalis、黄斑病Cercospora mesicaginis,炭疽病Colletotrichum trifolii、根腐病Rhizoctonia cyocorum 7种.苜蓿虫害主要有斜纹夜蛾、潜叶蝇、苜蓿蚜虫、蓟马4种.并在干旱半干旱、高寒阴湿和川塬灌区,根据不同地区苜蓿病虫害的发生及危害,开展了防治试验,提出了结合临夏地区生产实际防治苜蓿病虫害的对策.

  1. Decomposition of corn and soybean residues under field conditions and their role as inoculum source

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    E.M. Reis

    2011-03-01

    Full Text Available Necrotrophic parasites of above-ground plant parts survive saprophytically, between growing seasons in host crop residues. In an experiment conducted under field conditions, the time required in months for corn and soybean residues to be completely decomposed was quantified. Residues were laid on the soil surface to simulate no-till farming. Crop debris of the two plant species collected on the harvesting day cut into pieces of 5.0cm-long and a 200g mass was added to nylon mesh bags. At monthly intervals, bags were taken to the laboratory for weighing. Corn residues were decomposed within 37.0 months and those of soybean, within 34.5 months. Hw main necrotrophic fungi diagnosed in the corn residues were Colletotrichum gramicola, Diplodia spp. and Gibberella zeae, and those in soybeans residues were Cercospora kikuchii, Colletotrichum spp, Glomerella sp. and Phomopsis spp. Thus, those periods shoulb be observed in crop rotation aimed at to eliminating contaminated residues and, consequently, the inoculum from the cultivated area.

  2. Influence of Cultural and Pest Management Practices on Performance of Runner, Spanish, and Virginia Market Types in North Carolina

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    Bridget R. Lassiter

    2016-01-01

    Full Text Available Virginia market type peanut (Arachis hypogaea L. cultivars are grown primarily in North Carolina, South Carolina, and Virginia in the US, although growers in these states often plant other market types if marketing opportunities are available. Information on yield potential and management strategies comparing these market types is limited in North Carolina. In separate experiments, research was conducted to determine response of runner, Spanish, and Virginia market types to calcium sulfate and inoculation with Bradyrhizobium at planting, planting and digging dates, planting patterns, and seeding rates. In other experiments, control of thrips (Frankliniella spp. using aldicarb, southern corn rootworm (Diabrotica undecimpunctata Howardi using chlorpyrifos, eclipta (Eclipta prostrata L. using threshold-based postemergence herbicides, and leaf spot disease (caused by the fungi Cercospora arachidicola and Cercosporidium personatum fungicide programs was compared in these market types. Results showed that management practice and market types interacted for peanut pod yield in only the planting date experiment. Yield of runner and Virginia market types was similar and exceeded yield of the Spanish market type in most experiments.

  3. Utilization of induced mutations for groundnut breeding in Uganda

    International Nuclear Information System (INIS)

    Groundnuts (Arachis hypogaea L.) are on high demand in Uganda. There is, therefore, an urgent need to improve groundnut yields through breeding. The main objectives besides yield are the following: 1. To improve disease resistance: (a) rosette virus transmitted by aphids (Aphis craccivora); (b) leafspot caused by Cercospora arachidicola (early) and Cercosporidium personatum (late). 2. To advance the maturity period of high yielding varieties so as to fit better into the rainfall pattern of the main growing areas. 3. To improve seed uniformity, seed size and quality (protein, oil). 4. To reduce plant height by shortening the internodes so as to have more flower production near the ground. For mutation breeding three erect groundnut cultivars were used, Roxo a recommended commercial variety; Red Beauty (Bl) a recommended local variety and No. 534 a tan skinned variety. Seeds of the three varieties were irradiated in 1976 at the FAO/IAEA Agricultural Section of the IAEA Laboratory Seibersdorf, with 1500 rad of fast neutrons (Nf) or 20 krad of 60Co gamma rays. The pedigree method of selection was used until M9. During 1985 and 1986, seven mutant selections of Red Beauty and one from Roxo were tested in replicated yield trials. Results are given. On the basis of plot yields some of the Red Beauty mutant lines outyielded the parent but not the commercial variety Roxo

  4. Genetic characterization and linkage disequilibrium mapping of resistance to gray leaf spot in maize (Zea mays L.

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    Liyu Shi

    2014-04-01

    Full Text Available Gray leaf spot (GLS, caused by Cercospora zeae-maydis, is an important foliar disease of maize (Zea mays L. worldwide, resistance to which is controlled by multiple quantitative trait loci (QTL. To gain insights into the genetic architecture underlying the resistance to this disease, an association mapping population consisting of 161 inbred lines was evaluated for resistance to GLS in a plant pathology nursery at Shenyang in 2010 and 2011. Subsequently, a genome-wide association study, using 41,101 single-nucleotide polymorphisms (SNPs, identified 51 SNPs significantly (P < 0.001 associated with GLS resistance, which could be converted into 31 QTL. In addition, three candidate genes related to plant defense were identified, including nucleotide-binding-site/leucine-rich repeat, receptor-like kinase genes similar to those involved in basal defense. Two genic SNPs, PZE-103142893 and PZE-109119001, associated with GLS resistance in chromosome bins 3.07 and 9.07, can be used for marker-assisted selection (MAS of GLS resistance. These results provide an important resource for developing molecular markers closely linked with the target trait, enhancing breeding efficiency.

  5. Genetic characterization and linkage disequilibrium mapping of resistance to gray leaf spot in maize(Zea mays L.)

    Institute of Scientific and Technical Information of China (English)

    Liyu; Shi; Xiangling; Lv; Jianfeng; Weng; Hanyong; Zhu; Changlin; Liu; Zhuanfang; Hao; Yu; Zhou; Degui; Zhang; Mingshun; Li; Xiaoke; Ci; Xinhai; Li; Shihuang; Zhang

    2014-01-01

    Gray leaf spot(GLS),caused by Cercospora zeae-maydis,is an important foliar disease of maize(Zea mays L.)worldwide,resistance to which is controlled by multiple quantitative trait loci(QTL).To gain insights into the genetic architecture underlying the resistance to this disease,an association mapping population consisting of 161 inbred lines was evaluated for resistance to GLS in a plant pathology nursery at Shenyang in 2010 and 2011.Subsequently,a genome-wide association study,using 41,101 single-nucleotide polymorphisms(SNPs),identified 51 SNPs significantly(P<0.001)associated with GLS resistance,which could be converted into 31 QTL.In addition,three candidate genes related to plant defense were identified,including nucleotidebinding-site/leucine-rich repeat,receptor-like kinase genes similar to those involved in basal defense.Two genic SNPs,PZE-103142893 and PZE-109119001,associated with GLS resistance in chromosome bins 3.07 and 9.07,can be used for marker-assisted selection(MAS)of GLS resistance.These results provide an important resource for developing molecular markers closely linked with the target trait,enhancing breeding efficiency.

  6. Nuevo cultivar de soja de la EEAOC: Yanasu RR New soybean cultivar released by the EEAOC: Yanasu RR

    Directory of Open Access Journals (Sweden)

    José R. Sánchez

    2012-06-01

    Full Text Available Durante las últimas décadas, el cultivo de la soja a nivel mundial tuvo un crecimiento tanto en superficie, como en productividad. En la Argentina sucedió de igual manera, particularmente a comienzos de este siglo, como resultante del uso de alta tecnología, convirtiendo a este cultivo y a sus subproductos en el pilar del sistema granario y exportador. El desarrollo del Noroeste Argentino (NOA coincidió con el proceso a nivel nacional. La Estación Experimental Agroindustrial Obispo Colombres (EEAOC de Tucumán buscó permanentemente producir cultivares adaptados a esta zona, siendo este el principal objetivo del subprograma Mejoramiento Genético del Proyecto Soja. Yanasu es una nueva variedad de soja liberada por la EEAOC, que completa su madurez fisiológica a los 123 días de su siembra (pertenece al grupo de madurez VIII corto y que logra excelentes potenciales de rendimiento. Fue obtenida a través de cruzamiento y posterior avance generacional en la subestación de Monte Redondo (San Agustín, Tucumán. Yanasu RR fue sometida a ensayos comparativos de rendimiento (ECR en cuatro diferentes sitios del área sojera tucumana durante la campañas 2005/2006 y 2006/2007, alcanzando rendimientos superiores a los testigos en la mayoría de los ensayos. Exhibe, entre otras características, baja o nula dehiscencia de vainas y un peso de 1000 semillas de 162,6 gramos. En cuanto a su sanidad, se destaca su tolerancia a las enfermedades cancro del tallo y mancha ojo de rana, así como ante patógenos de semillas como Cercospora kikuchii, Alternaria spp. y Macrophomina spp. Desde 2009/2010 se la incluyó en la Red de Evaluación de Variedades Comerciales del NOA, destacándose por encima del testigo en 12 localidades de la región, con un excelente potencial de rendimiento.In recent decades, soybean planted area, production, and productivity have increased worldwide. This has also occurred in Argentina, especially during the last century, based on

  7. Potencial produtivo de linhagens de amendoim do grupo ereto precoce com e sem controle de doenças foliares Yield potential of upright early maturing peanut lines with and without control of foliar diseases

    Directory of Open Access Journals (Sweden)

    IGNÁCIO JOSÉ DE GODOY

    2001-01-01

    Full Text Available Avaliaram-se o potencial produtivo, o rendimento de grãos após descascamento e a massa de grãos de onze linhagens de amendoim (Arachis hypogaea L. do programa de melhoramento do Instituto Agronômico e da cultivar IAC-Tatu-ST, com e sem controle de doenças foliares. O material genético classifica-se no grupo ereto precoce (A. hypogaea subsp. fastigiata, Waldron. Seis experimentos foram desenvolvidos na época das águas, no Núcleo de Agronomia da Alta Mogiana e na Estação Experimental de Agronomia de Pindorama, em 1994/95, 1995/96 e 1996/97, em esquema com duas parcelas subdivididas, em blocos ao acaso com quatro repetições, em que as parcelas principais consistiram em tratamentos com e sem o controle químico das doenças. As parcelas tratadas foram pulverizadas periodicamente com 3,0 L.ha-1 do fungicida chlorothalonil para controle da mancha-castanha e da mancha-preta (Cercospora arachidicola e Cercosporidium personatum e da verrugose (Sphaceloma arachidis. A ausência de controle químico resultou em significativas reduções, até de 60%, na produção de todos os genótipos. Com as doenças controladas, as linhagens IAC 5, IAC 21, IAC 22 e IAC 81-12 apresentaram maior potencial produtivo, atingindo 5.000 kg.ha-1 em dois dos experimentos. As linhagens IAC 81-7 e IAC 88-1 apresentaram os melhores desempenhos relativos entre as médias de ambientes com e sem controle químico. As doenças causaram reduções até de 14% no peso médio dos grãos, sobretudo nas linhagens IAC 5, IAC 21 e IAC 22, cujos grãos são de maior tamanho médio. Não foram observadas diferenças significativas em rendimento de grãos entre os tratamentos, na maioria dos ambientes; essa característica mostrou, também, ser pouco influenciada pela presença das doenças.Eleven peanut lines and the cultivar IAC-Tatu-ST, of the upright early maturing group (Arachis hypogae subsp. fastigiata, Waldron, were evaluated for pod yield, shelling percentage and average

  8. Synthesis and Antifungal Activity of Camphoric Acid-based Thiourea Derivatives%樟脑酸基硫脲类化合物的合成及抑菌活性研究

    Institute of Scientific and Technical Information of China (English)

    马献力; 刘陆智; 段文贵; 石贤春; 李芳耀; 华忠泽

    2015-01-01

    Ten novel camphoric acid-based thiourea compounds (5a 5j) were prepared using camphoric acid and aminoethyl substituted phenyl thioureas as starting materials. The target compounds were characterized by FT-IR,1H NMR,13 C NMR,LC-MS,and elemental analysis. The antifungal activities of the compounds 5a 5j were tested. The preliminary bioassay showed that most of the target compounds exhibited certain antifungal activities,in which camphoric acid-based (o-methoxy)phenyl thiourea 5c (R=o-CH3O) and camphoric acid-based (m-methoxy)phenyl thiourea 5d (R=m-CH3O) had inhibition rate of 86. 9 % against Alternaria solani,and camphoric acid-based (m-methoxy)phenyl thiourea 5d,camphoric acid-based (m-methyl)phenyl thiourea 5g (R=m-CH3) and camphoric acid-based (p-bromo)phenyl thiourea 4i ( R=p-Br) had inhibition rate of 86. 1% against Physalospora piricola at the concentration of 50 mg/L. In addition,compound 5i also had the best inhibitory activity against cercospora arachidicola(73. 6%).%以樟脑酸和氨乙基取代苯基硫脲为原料,合成了10个新型的樟脑酸基硫脲类化合物(5a 5j). 利用FT-IR、1 H NMR、13 C NMR、LC-MS和元素分析等手段对目标产物进行了结构表征. 同时对化合物5a 5j的抑菌活性进行了测试. 初步的生物活性测试表明,在质量浓度为50 mg/L时,大部分化合物具有一定的抑菌活性,其中化合物樟脑酸基邻甲氧基苯基硫脲5c(R=o-CH3O)和樟脑酸基间甲氧基苯基硫脲5d(R=m-CH3O)对番茄早疫病菌(Alternaria solani)的抑制率达86. 9 %,化合物樟脑酸基间甲氧基苯基硫脲5d、樟脑酸基间甲基苯基硫脲5g( R=m-CH3 )和樟脑酸基对溴苯基硫脲5i(R=p-Br)对苹果轮纹病菌(Physalospora piricola)的抑制率达86. 1 %. 此外,化合物5i还对花生褐斑病毒(Cercospora arachidicola)具有最好的抑制率(73. 6%).

  9. Improvement of pigenonpea and cowpea for drought, disease and insect pest tolerance through induced mutations

    International Nuclear Information System (INIS)

    Pigeonpea and cowpea are widely grown in the semi-arid and arid regions of Kenya by small scale farmers. The average yields are usually low due to insect pests, diseases and long growth duration of the local land races. Little success has been achieved through conventional breeding methods for tolerance to insect pests and diseases despite the development of high yielding and early maturing lines. Therefore, mutation induction was initiated to widen the genetic variability in the improved lines. Seeds of three promising pigeonpea cultivars KAT 60/8, KAT 777 and KAT E31/4 and of cowpea KAT 419, K80 and M66 were subjected to three doses of gamma rays; 80, 120 and 150 Gy for pigeonpea and 160, 200 and 250 Gy for cowpea. In M1 generation, doses of 150 Gy and 250 Gy reduced emergence by about 50% and increased seedling deformities in both crops. In M2 generation of KAT 60/8, high yielding mutants with oval shaped seeds (T1 P58) and branching (T3 P28) were identified. Two progenies of KAT 777 (T1 P7 and T1 P11) had small slender leaves. Selected plant progenies in M3, M4 and M5 generation gave some promising high yielding variants. Although, the difference in days to flower and maturity of mutant progenies and untreated bulk were small, some mutant progenies of KAT 777 and KAT 60/8 showed tolerance to Fusarium wilt. None of the progenies of KAT E31/4 gave better score for Cercospora leaf-spot compared to the check. (author). 2 refs, 4 tabs

  10. Unravelling Mycosphaerella: do you believe in genera?

    Science.gov (United States)

    Crous, P W; Summerell, B A; Carnegie, A J; Wingfield, M J; Hunter, G C; Burgess, T I; Andjic, V; Barber, P A; Groenewald, J Z

    2009-12-01

    Many fungal genera have been defined based on single characters considered to be informative at the generic level. In addition, many unrelated taxa have been aggregated in genera because they shared apparently similar morphological characters arising from adaptation to similar niches and convergent evolution. This problem is aptly illustrated in Mycosphaerella. In its broadest definition, this genus of mainly leaf infecting fungi incorporates more than 30 form genera that share similar phenotypic characters mostly associated with structures produced on plant tissue or in culture. DNA sequence data derived from the LSU gene in the present study distinguish several clades and families in what has hitherto been considered to represent the Mycosphaerellaceae. In some cases, these clades represent recognisable monophyletic lineages linked to well circumscribed anamorphs. This association is complicated, however, by the fact that morphologically similar form genera are scattered throughout the order (Capnodiales), and for some species more than one morph is expressed depending on cultural conditions and media employed for cultivation. The present study shows that Mycosphaerella s.s. should best be limited to taxa with Ramularia anamorphs, with other well defined clades in the Mycosphaerellaceae representing Cercospora, Cercosporella, Dothistroma, Lecanosticta, Phaeophleospora, Polythrincium, Pseudocercospora, Ramulispora, Septoria and Sonderhenia. The genus Teratosphaeria accommodates taxa with Kirramyces anamorphs, while other clades supported in the Teratosphaeriaceae include Baudoinea, Capnobotryella, Devriesia, Penidiella, Phaeothecoidea, Readeriella, Staninwardia and Stenella. The genus Schizothyrium with Zygophiala anamorphs is supported as belonging to the Schizothyriaceae, while Dissoconium and Ramichloridium appear to represent a distinct family. Several clades remain unresolved due to limited sampling. Mycosphaerella, which has hitherto been used as a term of

  11. 国内木薯病害普查及细菌性萎蔫病安全性评估%General Survey on Cassava Diseases and Safety Assessment of Cassava Bacterial Blight

    Institute of Scientific and Technical Information of China (English)

    李超萍; 时涛; 刘先宝; 蔡吉苗; 裴月令; 黄贵修

    2011-01-01

    2007~2010年,对广西、海南、云南等木薯主产区进行病害普查.结果发现,至今危害国内木薯的主要病害有7种,包括真菌病害6种[褐斑病(Cercosporidium henningsii)、炭疽病(colletotrichum gloeosPorioides)、离孺孢叶斑病(Bipolaris setariae)、棒孢霉叶斑病(Corynespora cassiicola)、白点病(phaeoramularia manihotis)和萎蔫叶斑病(Cercospora vicosae)],细菌病害1种[细菌性萎蔫病(Xanthomonas axonopodis pv manihotis)],其中离孺孢叶斑病和棒孢霉叶斑病为新发病害;7种病害中褐斑病和细菌性萎蔫病发生面积最大,危害最为严重.风险评估结果表明,细菌性萎蔫病菌为高度危险性有害生物.%During 2007 to 2010, cassava(Manihot esculenta Crantz)diseases survey was conducted in Guangxi, Hainan and Yunnan, the main cassava planting areas of China. The results showed that only seven diseases, six fungal diseases and one bacterial disease, were found. The cassava brown leaf spot caused by Cercosporidium henningsii and the bacterial blight caused by Xanthomonas axonopodis pv Manihotis prevailed in most cassava planting areas and caused serious damage. The safety assessment indicated that the cassava bacterial blight was a high risk disease. At present, cassava diseases are becoming one of the most important limiting factors for the development of cassava industry and would have a very serious,potential economic risk on correlative industry and the ecology of the tropic area.

  12. Características agronómicas y sanidad de germoplasma promisorio de maní (Arachis hypogaea L. en Quevedo, Ecuador

    Directory of Open Access Journals (Sweden)

    Felipe Rafael Garcés-Fiallos

    2014-12-01

    Full Text Available Durante las épocas secas de 2010 y 2011, en la finca experimental La María de la Universidad Técnica Estatal de Quevedo, Ecuador, localizada en el km. 7.5 vía Quevedo-El Empalme, a 79° 30' 08" O y 01° 00' 35" S, se evaluaron las características agronómicas y las condiciones sanitarias de líneas y variedades de maní (Arachis hypogaea L.. Se utilizaron las líneas promisorias de maní CB-02, CB-05, CB-12, CB-13, CB-15, CB-16 y CB-23 de la Universidad y como testigos las variedades comerciales Diamante, Polachi, INIAP-380 e INIAP-381. Se midieron la emergencia de plántulas, días a floración, altura de planta, algunos componentes productivos (número de granos, frutos llenos y estériles, peso de frutos y granos y la presencia de cercosporiosis (Cercospora arachidicola y Cercosporidium personatum y roya (Puccinia arachidis. Se utilizó un diseño de bloques completos al azar (DBCA con 11 tratamientos y tres repeticiones en 2010 y cuatro repeticiones en 2011. Para las comparaciones entre las medias de los tratamientos se empleó la prueba de rangos múltiples de Duncan (P < 0.05. La línea CB-23 se destacó entre el germoplasma promisorio por su mayor productividad; esta misma línea junto con la variedad comercial INIAP-380, presentaron los menores ataques de cercosporiosis. La línea CB-15 y la variedad Polachi no presentaron lesiones de la roya.

  13. Purification and characterisation of an antifungal protein, MCha-Pr, from the intercellular fluid of bitter gourd (Momordica charantia) leaves.

    Science.gov (United States)

    Zhang, Beibei; Xie, Chengjian; Wei, Yunming; Li, Jing; Yang, Xingyong

    2015-03-01

    An antifungal protein, designated MCha-Pr, was isolated from the intercellular fluid of bitter gourd (Momordica charantia) leaves during a screen for potent antimicrobial proteins from plants. The isolation procedure involved a combination of extraction, ammonium sulphate precipitation, gel filtration on Bio-Gel P-6, ion exchange chromatography on CM-Sephadex, an additional gel filtration on HiLoad 16/60 Superdex 30, and finally, HPLC on a SOURCE 5RPC column. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry indicated that the protein had a molecular mass of 25733.46Da. Automated Edman degradation was used to determine the N-terminal sequence of MCha-Pr, and the amino acid sequence was identified as V-E-Y-T-I-T-G-N-A-G-N-T-P-G-G. The MCha-Pr protein has some similarity to the pathogenesis-related proteins from Atropa belladonna (deadly nightshade), Solanum tuberosum (potato), Ricinus communis (castor bean), and Nicotiana tabacum (tobacco). Analysis of the circular dichroism spectra indicated that MCha-Pr predominantly contains α-helix and β-sheet structures. MCha-Pr had inhibitory effects towards a variety of fungal species and the 50% inhibition of fungal growth (IC50) for Alternaria brassicae, Cercospora personata, Fusarium oxysporum, Mucor sp., and Rhizoctonia solani are 33 μM, 42 μM, 37 μM, 40 μM, and 48 μM, respectively. In addition, this antifungal protein can inhibit the germination of A. brassicae spores at 12.5 μM. These results suggest that MCha-Pr in bitter gourd leaves plays a protective role against phytopathogens and has a wide antimicrobial spectrum. PMID:25245535

  14. Increased levels of ambient fungal spores in Taiwan are associated with dust events from China

    Science.gov (United States)

    Wu, Pei-Chih; Tsai, Jui-Chen; Li, Fang-Chun; Lung, Shih-Chun; Su, Huey-Jen

    2004-09-01

    Fungi are ubiquitous in nature and their spores are often dispersed into the atmosphere through turbulent airstreams. As yellow sandstorm blown from deserts in China had affected the ambient air quality with increasing levels of ambient particulates, often including significant amounts of biologically active particles has therefore become imperative for concerns of their health implications. Our study was aimed to examine the effects of yellow sandstorm events on the fungal composition and concentrations in ambient air. Atmospheric fungal spores were continuously collected using Burkard Volumetric Spore Trap. Samples collected between December 2000 and April 2001 were selected for priority analysis from days when the yellow sandstorms were reported to affect Taiwan according to the Central Weather Bureau in Taiwan. The composition of dominant spores such as Basidiospore, Penicillium/Aspergillus, Nigrospora, Arthrinium, Curvularia, Rusts, Stemphylium, Cercospora, Pithomyces, and unidentified fungi were significantly higher than those of background days. The increase of Basidiospore, Penicillium/Aspergillus, Nigrospora, and those unidentified fungi seems to be significantly associated with the increase of ambient particulate levels with regression coefficients ranging from 0.887 to 31.98. Our study has identified increasing ambient concentrations during sandstorm episodes are observed for some major fungi, Basidiospore, Penicillium, Aspergillus, and those unidentified fungi and the trends of the increase seems to associate with ambient particulate levels. Further efforts to clarify the relationship between those high fungal spore exposures and clinical adverse health effects are suggested in the future. In addition, effects of climatic factors and other particulate levels on the variation of ambient fungal spore levels are also desired in further study. Additional monitoring of ambient fungal spores in the first line of west coastline is hoped to assist in

  15. Influência da adubação nitrogenada, época de plantio e aerosporos sobre a severidade da mancha de grãos em arroz de terras altas

    Directory of Open Access Journals (Sweden)

    Valácia Lemes da Silva-Lobo

    2011-09-01

    Full Text Available A mancha de grãos (MG ocupa o segundo lugar em importância econômica entre as doenças do arroz. Foi estudada a influência de níveis de adubação nitrogenada, época de plantio e concentração de esporos no ar sobre a severidade da doença no campo. A severidade de MG, na cultivar BRS Bonança, foi avaliada em duas épocas de plantio (30/11/2006 e 21/12/2006 e cinco doses de adubação nitrogenada (0, 30, 60, 120 e 240 kg de N.ha-1 utilizando o delineamento experimental de blocos ao acaso em esquema de parcelas subdivididas com três repetições. O efeito de dose de N sobre a severidade de MG não foi significativo. A correlação entre a severidade de MG e espiguetas vazias foi positiva e significativa. A população de fungos no ar (aerosporos foi quantificada utilizando armadilhas volumétricas, Rotorod Sampler, desde a emissão até o amadurecimento das panículas. A mancha de grãos aumentou linearmente com tempo (r = 0,98; P < 0,01, o mesmo não ocorreu com o aumento total de fungos que variou de 0,23 a 2,97 esporos/litro de ar/minuto. Os fungos presentes no ar em ordem decrescente de concentração foram Nigrospora sp., Pyricularia oryzae, Pithomyces sp., Alternaria sp., Cercospora sp., Fusarium sp., Curvularia sp. e Bipolaris sp. Estes fungos e Phoma sp. entre outros também foram detectados no teste de sanidade de sementes. A correlação entre a quantidade de esporos de P. oryzae e outros fungos foi linear e positiva (r = 0,80, P < 0,01. O número de esporos aumentou com o aumento da umidade relativa e diminuiu com o aumento da temperatura máxima de maneira exponencial.

  16. Validação de dois sistemas de previsão para a queima das folhas da cenoura

    Directory of Open Access Journals (Sweden)

    SOUZA ROSEMARI T.

    2002-01-01

    Full Text Available Através de quatro experimentos realizados em campo, no período de setembro/98 a dezembro/99, avaliou-se a aplicabilidade de dois sistemas de previsão no controle da queima das folhas da cenoura (Daucus carotae. Seis esquemas de aplicação de fungicida (protetor ou sistêmico, baseados no acúmulo de valores diários de severidade (VDS para Alternaria dauci ou equivalentes de infecção (EI para Cercospora carotae, foram comparados a dois calendários fixos de tratamento e uma testemunha sem fungicida. Enquanto o número de VDS registrados por um coletor eletrônico (Colpam variou de 25 a 94 por ciclo da cultura, nenhum EI foi detectado ao longo dos quatro experimentos. Com a adoção do sistema de VDS, o número de aplicações do fungicida protetor clorotalonil diminuiu de 7,5 (fixo, sete dias para 3,5 (VDS 3-5, 2,7 (VDS 6-8 e dois (VDS 9-11 por cultivo. Nas mesmas condições, o fungicida sistêmico metconazole foi aplicado 3,7 (fixo, 14 dias, 2,5 (VDS 3-5, 1,7 (VDS 6-8 e 1,5 (VDS 9-11 vezes. A relação entre a área abaixo da curva de progresso de doença (AACPD e o peso de raiz foi significativa em dois experimentos (P = 0,005 e 0,016. Quanto ao rendimento de raízes, o limiar de 3-5 VDS igualou-se ao esquema de calendário fixo em 3/4 das situações. Portanto, nas condições locais, o controle da queima das folhas pelo sistema de VDSs está condicionado ao limite de 3-5 VDS e à utilização de fungicidas sistêmicos com eficácia igual ou superior à do metconazole.

  17. Effects of ultraviolet radiation on Hibiscus rosa-sinensis, Beta vulgaris and Helianthus annuus

    International Nuclear Information System (INIS)

    It is believed that increased levels of ultraviolet B-radiation (UV-B;280-320 nm) will result in serious threat to plant. In the present study the effects of UV (particularly UV-B) were studied on chlorophyll fluorescence, ultraweak luminescence (UL) and plant growth. Parameters related to light emission were determined, and the effects of UV-B on hypocotyl elongation and levels of free IAA were examined. The plants were grown in greenhouse or in growth chambers and exposed to short or long term UV-B simulating different levels of ozone depletion. Short exposure of Hibiscus leaves to UV resulted in a gradual increase in both UL and peroxidase activity followed by a decline after 72 h and a decrease in variable chlorophyll fluorescence. The action of UV-B on sugar beet plants depended on light quality and irradiance and infection by Cercospora beticola Sacc. The interaction between UV-B and the disease resulted in a large reduction of dry weight and enhanced UL. The lowest Chl a and growth was found in plants grown under low irradiance and exposed to UV-B supplemented with UV-A (320-400 nm). UVB also inhibited photosystem II, increased UL and peroxidase activity. Under relatively high PAR, UV-B increased dry weight of laminae and UL but no effect on Chl content. Sugar beet plants grown with light depleted in the 320-400 nm region of the spectrum and exposed to UV-B died. Low levels of UV-B did neither affected hypocotyl elongation nor amounts of free IAA in sunflower plants grown under low (LL; 143 μmol m-2s-1) or high PAR (HL; 800 μmol m-2s-1). Three times more daily UV-B increased the amount of free IAA, but inhibited hypocotyl elongation. Higher Fv/Fmax and F690/F735, Chl a and carotenoids were found in plants exposed to low UV-B. Indeed, UV-B can be harmful but may also have enhancing effects on plants. (au) (114 refs.)

  18. The phytosanitary form and fighting measures diseases and pests of sugar beet from Republic of Moldova

    Directory of Open Access Journals (Sweden)

    Timus Asea M.

    2006-01-01

    Full Text Available Sugar beet is one of the most important agricultural crops in the Republic of Moldova. The North and Central regions have good enough pedoclimatic conditions. The genetic potential of sorts and hybrids can be created through the application of modern technologies in order to grow at least 32-35 tons/ha of sugar beet roots. In the Republic of Moldova, sugar beet vegetates between 160 and 180 days in the first year and needs approximately an amount of 2400-2900°C, average of 15.3-15.4°C. Each phenological phase needs different temperatures: at least 4C° is necessary for planting and springing an amount of 650°C is necessary for foliar apparatus; an amount of 1150- 1800°C is necessary to grow the volume of roots and for sugar depositing the average of 2400 to 2600°C is necessary. The mentioned temperatures ensure a normal development of sugar beet plants. If these temperatures fluctuate, the pathogens and pests are stimulated to develop. The most frequent diseases of sugar beet are: Pythium de baryanum Hesse., Aphanomyces cochlioides Dresch. Peronospora schachtii Fuck., Phoma betae Fr. Cercospora beticola Sacc. Erysiphe communis Grev. f. betae Jacz., virosis - Beta virus 2, 3 si 4 etc. The main pests belong to the following categories: Homoptera: Aphis fabae Scop. (fam. Aphididae, Pemphigus fuscicornis Koch. (fam. Pemphigidae Coleoptera: Agriotes sp. (fam. Elateridae, Chaetocnema concinna M. Ch. breviuscula Fld., Cassida nebulosa L. (fam. Chrysomelidae, Atomaria linearis Step. (fam. Cryptophagidae; Bothynoderes punctiventris Germ., Tanymechus dilaticollis Gyll., T. palliatus F., Psalidium maxillosum F. (Curculionidae; Lepidoptera: Agrotis segetum Den. et Schiff., Authographa gamma L. Mamestra (Barathra brassicae L. (Noctuidae, Loxostege sticticalis L. (Pyralidae, Gnorimoschema ocellatella Boyd.; Diptera Pegomyia betae Curtis. (fam. Anthomyidae. Heterodera schachtii Schmidt (Heteroderidae. The most recommended insecticides for fighting the

  19. Produtividade, florescimento prematuro e queima-das-folhas em cenoura cultivada em sistema orgânico e convencional Yield, bolting and leaf blight in carrots grown under organic and conventional conditions

    Directory of Open Access Journals (Sweden)

    Assis M. Carvalho

    2005-06-01

    Full Text Available Quatro ensaios foram implantados no verão para avaliar a produtividade, o florescimento prematuro e a queima-das-folhas em genótipos de cenoura conduzidos em sistema orgânico e convencional. Os ensaios foram instalados em Brazlândia e na Embrapa Hortaliças. O delineamento experimental foi de blocos ao acaso, com oito tratamentos (genótipos Alvorada, Brasília RL, Brasília Bionatur, Kuronan, Nantes 3 Tip Top, Carandaí AG 106, Brazlândia e Pop. 0212246 e cinco repetições. Os nutrientes foram incorporados ao solo através de composto orgânico, no sistema orgânico e, fertilizantes químicos, no sistema convencional. Aos 70 dias da semeadura as plantas foram avaliadas no campo para incidência de doenças. Foi também identificada a prevalência de patógenos. A colheita foi realizada 95 dias após a semeadura. O florescimento prematuro foi mais freqüente no genótipo Brasília Bionatur, no sistema convencional, em Brazlândia. Foi observada diferença entre genótipos para queima-das-folhas nos dois sistemas. Nantes foi a mais suscetível, enquanto a Pop. 0212246 foi uma das mais resistentes nos dois sistemas de cultivo. Alternaria dauci prevaleceu em Brazlândia, enquanto Cercospora carotae foi observado em ambas as localidades, no sistema orgânico. Quanto à produção comercial e total os genótipos Pop. 0212246 e Brazlândia estiveram entre os mais produtivos, independente do sistema de cultivo. Para produtividade comercial e total, número e peso de raízes refugadas o sistema convencional apresentou resultados superiores ao orgânico. As cultivares Brasília RL, Brazlândia e a Pop. 0212246 podem ser recomendadas para plantio no período de verão, no DF, independente do sistema de cultivo. A Pop. 0212246 apresenta características agronômicas desejadas pelo mercado e poderá futuramente ser disponibilizada para produtores da região.Four experiments were carried out with carrots genotypes during summer season in Distrito

  20. Doenças da mandioquinha-salsa e sua situação atual no Brasil Present situation of arracacha (Arracacia xanthorrhiza diseases in Brazil

    Directory of Open Access Journals (Sweden)

    Gilmar P. Henz

    2002-06-01

    Full Text Available Este artigo tem como objetivo principal relatar as doenças registradas para a mandioquinha-salsa até a data presente e descrever sua situação atual no País. Na literatura, foram registrados para a cultura 27 gêneros de fungos, três de bactérias, doze de nematóides e cinco espécies de vírus. Destes, já foram relatados no Brasil treze fungos, e todos nematóides e bactérias, enquanto nenhum vírus foi oficialmente registrado, embora já tenham sido observadas plantas com sintomas típicos de viroses a campo. A maior parte dos registros estão na forma de resumos, capítulos de livros ou publicações genéricas, desprovidos de uma série de dados relevantes, como provas de patogenicidade, importância da doença, perdas, identificação mais acurada, entre outras. A cultura da mandioquinha-salsa ainda é tida como rústica, sendo poucas as doenças consideradas limitantes. O nematóide das galhas (Meloidogyne spp. e a podridão-mole pós-colheita causada por Erwinia spp. são os principais problemas atualmente, e causam perdas significativas. Também ocorrem com muita frequência manchas foliares causadas por Septoria, Cercospora e Xanthomonas campestris pv. arracaciae, e em algumas regiões podridão de plantas a campo causadas por Sclerotinia sclerotiorum e Sclerotium rolfsii. Como não existe nenhum produto químico oficialmente registrado para a mandioquinha-salsa no Ministério da Agricultura, Pecuária e Abastecimento, as medidas de controle passíveis de recomendação incluem ações preventivas, como o uso de material propagativo sadio (preferencialmente mudas pré-enraizadas de origem conhecida, a adoção de rotação de culturas, a eliminação de plantas ou partes doentes, e a adubação e a irrigação adequadas. O estudo sistematizado das doenças e o registro cuidadoso de surtos ou novas enfermidades para a cultura são importantes para subsidiar futuras medidas de controle.The diseases of arracacha that have already

  1. Efeito da aplicação de fungicida sobre caracteres agronômicos e severidade das doenças de final de ciclo na cultura da soja. = Effect of fungicide application on Agronomic characteristics and final cycle diseases severity in soybean.

    Directory of Open Access Journals (Sweden)

    Everton Luis Finoto

    2011-04-01

    Full Text Available Nas últimas safras, um complexo de doenças, chamadas de doenças de final de ciclo da soja (DFC, vem provocando reduções consideráveis no rendimento da cultura. Atualmente, não há variedades resistentes a todos os patógenos causadores das DFC. Objetivou-se no presente trabalho avaliar o efeito da aplicação de fungicida em diferentes estádios reprodutivos, nos caracteres agronômicos e severidade das DFC em três cultivares de soja de diferentes grupos de maturação. O trabalho foi conduzido em área experimental da Universidade Federal de Viçosa. Foram realizados quatro tratamentos com fungicida sendotrês aplicações em estádios reprodutivos diferentes (R5, R5,5 e R6 e a testemunha sem aplicação. As cultivares utilizadas foram: BRSMG Liderança, UFVS 2001 e UFVS 2003. Foi avaliado: altura da planta, altura da inserção da primeira vagem, número de nós por planta, número de vagens por planta, peso total da planta e peso de sementes. A severidade das DFC foi avaliada no estado R6 através de escala de notas. Os dados das avaliações foram submetidos aos testes de normalidade e homogeneidade constatando-se a desnecessidade de transformações. Realizou-se as análises de variância e as médias foram comparadas pelo teste de Tukey, a 5% de probabilidade de erro. Na avaliação da severidade das doenças, foi constatada a presença dos seguintes patógenos: Septoria glycines, Cercospora kikuchii e Peronospora manshurica. Para a avaliação das características agronômicas foi observado que a cultivar UFVS 2001 apresentou maiores alturas de planta. A cultivar UFVS 2003 apresentou maior númerode nós e também maior produtividade de grãos, seguida pela cultivar Liderança. A aplicação de fungicida proporcionou o controle satisfatório de Septoria glycines no folíolo, quando realizada no estádio R5. A aplicação do fungicida proporcionou também aumento na produtividade de grãos, sendo os melhores resultados obtidos

  2. Fungal pathogens intercepted in imported germplasm of medicinal and aromatic plants and their quarantine significance

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    Usha Dev

    2011-01-01

    -borne pathogen and potential mycoherbicide for opium poppy crop. Alternaria zinniae Pape ex. Ellis and Botrytis alli Munn having limited geographical distribution in the country; Fusarium oxysporum Schlecht., known to possess a number of physiological races; B. cinerea Pers. Fr., Botryodiplodia theobromae Pat., Colletotrichum dematium (Pers. Grove, C. gloeosporioides (Penz. Sacc., Drechslera sorokiniana (Sacc. Subram. & Jain, F moniliforme Sheldon, F solani (Mart. Sacc., Macrophomina phaseolina (Tassi Goid and Rhizoctonia solani Kuhn etc. having wide host range. Further Alternaria brassicicola (Schw. Wiltshire and F solani (Mart. Sacco on non-host crops and Ascochyta sp., A. linicola, A. ricini Groves & Skolko, Curvularia lunata (Wakker Boedjin, Cercospora traversciana Sacc., D. papaveris, Pestalotia sp. and Phomopsis phaseoli Sacco causing damage of economic significance also were intercepted. Introduction of these exotic pathogens or their more virulent strains along with germplasm could pose a serious threat to the indigenous crops. Interception of such a large number of pathogens signifies the role of quarantine in regulating the safe introduction of plant genetic resources needed for the crop improvement programmes of medicinal and aromatic plants of the country.

  3. 惠州西湖景区古树名木病虫害调查及保护建议%Investigations on the diseases and insect pests of the ancient and famous trees of West Lake scenic area in Huizhou and suggestions for their protection

    Institute of Scientific and Technical Information of China (English)

    钟平生; 汤玉娟; 张颂声

    2011-01-01

    [目的]了解广东惠州西湖景区古树名木病虫害发生情况,为西湖景区古树名木养护管理提供参考.[方法]采用普查和抽查相结合的方法,并参考相关文献,对惠州西湖景区内古树名木主要病虫害种类及危害程度进行调查和鉴定.[结果]西湖景区内古树名木共有8科8属9种31棵,病害共12种,其中危害叶片的病害主要有灰斑病、炭疽病、褐斑病、藻斑病、煤污病等,危害树干的病害主要有枝枯病、干腐病;虫害有6目14科19种,其中刺吸性害虫和食叶性害虫主要有榕管蓟马、绵蚧、荔蝽、榕卵痣木虱、天蛾、中国绿刺蛾、棉大卷叶蛾和丽绿刺蛾等,蛀干害虫主要有家白蚁、眉斑楔天牛和云斑天牛等.叶部病害、刺吸性害虫和食叶性害虫对植株的危害较轻,枝枯病、干腐病和家白蚁等对植株的危害较严重.[建议]加强宣传,提高人们对古树名木的保护意识;加强对古树名木的复壮修补及病虫害防治工作;采用科学技术,防控园林植物病虫害.%[Objective]The objective of the current study was to investigate the occurrence of diseases and insect pests of the ancient and famous trees in West Lake scenic area in Huizhou, and to provide reference for their scientific conservation and management. [Method]The main diseases and insect pests in the old trees and the grade of damages caused by them were investigated using methods of general investigation and random inspection and referring to the relevant literatures.[Result]Thirty--one old trees corresponding to 9 species, 8 genus, 8 families were found in West Lake scenic area. Twelve kinds of diseases, identified in these trees, which damaged leaves of old trees were Cercospora Sojina Hara, anthracnose,brown patch, Cephaleuros spp, sooty blotch, while the diseases which damaged stem were branch rot and dry rot. Nineteen species of insects corresponding to 14 families of 6 orders were found in scenic area, of