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Sample records for ceratopogonidae

  1. New records of biting midges from Argentina and Chile (Diptera: Ceratopogonidae Nuevos registros de Ceratopogonidae (Diptera para Argentina y Chile

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    Gustavo R. Spinelli

    2010-06-01

    Full Text Available New records are provided for 22 species of biting midges (Diptera: Ceratopogonidae from Argentina and Chile, 12 belonging to Forcipomyia Meigen, two to Atrichopogon Kieffer and Culicoides Latreille, and one to Leptoconops Skuse, Dasyhelea Kieffer, Paradasyhelea Macfie, Alluaudomyia Kieffer, Macrurohelea Ingram & Macfie and Palpomyia Meigen. Forcipomyia (F. harpegonata Wirth & Soria, F. (F. pictoni Macfie, F. (F. sexvittata Wirth, F. (Lepidohelea annulatipes Macfie, F. (L. kuanoskeles Macfie, F. (Phytohelea jocosa Saunders and F. (Thyridomyia nana (Macfie are firstly recorded from Argentina, and Leptoconops ricardoi Ronderos & Spinelli, Atrichopogon endemicus Spinelli & Marino, Culicoides chacoensis Spinelli & Wirth, Paradasyhelea brevipalpis (Ingram & Macfie and Alluaudomyia schnacki Spinelli are firstly recorded from Chile. Forcipomyia calchaqui Spinelli & Marino is removed from the subgenus Thyridomyia Saunders and assigned to the subgenus Synthyridomyia Saunders.Se brindan nuevas citas de 22 especies de Ceratopogonidae para Argentina y Chile, de las cuales 12 pertenecen a Forcipomyia Meigen, dos a Atrichopogon Kieffer y Culicoides Latreille, y una a Leptoconops Skuse, Dasyhelea Kieffer, Paradasyhelea Macfie, Alluaudomyia Kieffer, Macrurohelea Ingram & Macfie y Palpomyia Meigen. Forcipomyia (F. harpegonata Wirth & Soria, F. (F. pictoni Macfie, F. (F. sexvittata Wirth, F. (Lepidohelea annulatipes Macfie, F. (L. kuanoskeles Macfie, F. (Phytohelea jocosa Saunders y F. (Thyridomyia nana (Macfie son registradas por primera vez para Argentina, mientras que Leptoconops ricardoi Ronderos & Spinelli, Atrichopogon endemicus Spinelli & Marino, Culicoides chacoensis Spinelli & Wirth, Paradasyhelea brevipalpis (Ingram & Macfie y Alluaudomyia schnacki Spinelli lo son para Chile. Se excluye Forcipomyia calchaqui Spinelli & Marino del subgénero Thyridomyia Saunders y se la asigna a Synthyridomyia Saunders.

  2. Gene discovery and differential expression analysis of humoral immune response elements in female Culicoides sonorensis (Diptera: Ceratopogonidae)

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    Background: Female Culicoides sonorensis midges (Diptera: Ceratopogonidae) are vectors of pathogens that impact livestock and wildlife in the United States. Little is known about their molecular functioning, including components of their immune system. Because the insect immune response is involved ...

  3. Vector competence of Culicoides sonorensis (Diptera: Ceratopogonidae) to epizootic hemorrhagic disease virus serotype 7

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    Background: Culicoides sonorensis (Diptera: Ceratopogonidae) is a vector of epizootic hemorrhagic disease virus (EHDV) serotypes 1 and 2 in North America, where these viruses are well-known pathogens of white-tailed deer (WTD) and other wild ruminants. Although historically rare, reports of clinica...

  4. Transcriptome analyses of blood and sugar digestive processes in female Culicoides sonorensis midges (Diptera: Ceratopogonidae)

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    Female Culicoides sonorensis Wirth & Jones (Diptera:Ceratopogonidae) midges vector numerous diseases impacting livestock and humans. The molecular physiology of this midge has been under-studied, so our approach was to gain an understanding of basic processes of blood and sucrose digestion using tra...

  5. First detection of Leishmania infantum (Kinetoplastida: Trypanosomatidae) in Culicoides spp. (Diptera: Ceratopogonidae)

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    Slama, Darine; Haouas, Najoua; Remadi, Latifa; Mezhoud, Habib; Babba, Hamouda; Chaker, Emna

    2014-01-01

    Background Culicoides (Diptera: Ceratopogonidae) species are known to be the vectors of Bluetongue virus and African Horses Sickness virus (AHSV) in different areas of the world. Nevertheless, other researchers have hypothesized that these arthropods could be involved in the transmission of other pathogens such as Schmallenberg virus, Plasmodium and Leishmania parasites. Identification of the Culicoides’ potential vector competence is crucial in understanding the worldwide Culicoides/Leishman...

  6. Two new species of the subgenus Acanthohelea of Stilobezzia from Brazilian Amazonia (Diptera: Ceratopogonidae).

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    Cazorla, Carla G; Spinelli, Gustavo R

    2016-01-01

    The predaceous genus Stilobezzia Kieffer is a large, diverse group of Ceratopogonidae that is worldwide in distribution except for Antarctica and some islands (Borkent 2014). Adult females are important predators on other small insects, and the immature stages are found in a wide variety of aquatic and semiaquatic habitats, including streams, lakes and pond margins, puddles, swamps, rice fields, rock pools, and tree holes (de Meillon and Wirth 1991; Cazorla et al. 2006). PMID:27395546

  7. Comparison of emergence traps of different shape and translucency in the trapping of Culicoides (Diptera: Ceratopogonidae).

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    Steinke, S; Lühken, R; Kroischke, F; Timmermann, E; Kiel, E

    2016-06-15

    Various types of emergence traps are available for investigations of the breeding habitats of Culicoides (Diptera: Ceratopogonidae). In order to assess the potential impact of the trap design on the trapping success, we compared the efficiency of opaque and white (more translucent) emergence traps and two trap shapes (cone-shaped and quadratic), to sample Culicoides emerging from cowpats. Significantly higher numbers of Culicoides chiopterus and Culicoides dewulfi were trapped with opaque traps, while there was no obvious effect of the trap shape. There were no distinct differences in the microclimate among different trap types. PMID:27198792

  8. Wing pattern variation in the Patagonian biting midge, Forcipomyia (Forcipomyia multipicta Ingram & Macfie (Diptera, Ceratopogonidae

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    Gustavo R. SPINELLI

    2012-01-01

    Full Text Available Examination of the type-series and non-type specimens of the Patagonian biting midge, Forcipomyia (Forcipomyia multipicta Ingram & Macfie (Diptera: Ceratopogonidae, revealed considerable variation in wing patterns of both sexes. One pattern includes several distinct light spot areas, whereas another pattern (e.g, in the holotype only features marginal light spots in cell r3, while other light spots are barely perceptible or absent. The cause(s of the differential lack of dark macrotrichia in certain areas of the wing membrane in specimens of some series could not be attributed either to their age, sex, or method of preservation.

  9. Culicoides hildebrandoi, a new species of the reticulatus species group from the Brazilian Amazon Region (Diptera, Ceratopogonidae).

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    Farias, Emanuelle de Sousa; Pereira Júnior, Antonio Marques; Felippe-Bauer, Maria Luiza; Pessoa, Felipe Arley Costa; Medeiros, Jansen Fernandes; Santarém, Maria Clara Alves

    2016-01-01

    A new species of biting midge (Diptera: Ceratopogonidae), Culicoides hildebrandoi sp. n., is described and illustrated based on female and male specimens from the states of Amazonas and Rondônia, Brazil. This new species belongs to the reticulatus species group and differs from the 24 other species of this group by the elongate slightly swollen 3(rd) palpal segment with scattered capitate sensilla but lacking a sensory pit. PMID:27110160

  10. 新疆吸血蠓虫的区系研究%Fauna of Ceratopogonidae in Xinjiang

    Institute of Scientific and Technical Information of China (English)

    田原; 刘增加; 贾蕾

    2011-01-01

    目的 研究新疆蠓科吸血种类的区系.方法 网捕法、畜诱法、灯诱法、人帐诱法和文献资料收集.结果 新疆67种吸血蠓的构成,细蠓属17种,蠛蠓属2种和库蠓属48种.以库蠓属种类最多,占已知种类的71.64%;细蠓属种类次之,占25.37%;蠛蠓属种类数最少,仅占已知种类的2.99%.地理分布,除乌鲁木齐市外,其余各地区均有蠓种分布,以伊犁地区和阿克苏市蠓种最多.已知67种蠓的区系属性,属于古北界51种,两界共有16种,未见东洋界种类.结论 为蠓科昆虫区系研究提供了科学依据.%Objective To study fauna distribution of Ceratopogonidae in Xinjiang. Methods Using bedent snars, lamp snars, animal snars and insect net to collect Ceratopogonidae from Xinjiang. Results Ceratopogonidae of Xinjiang belonging to 3 genera and a total of 67 species had been recorded, of which 17 species of Leptoconops Skuse, 2 species of Lasiohelea Kieffer and 48 species of Culicoides Latreille. Culicoides Latreille was superior in species number(71.64% ) ,the second was Leptoconops in species number(25. 37% )and the third was Lasiohelea Kieffer in species number(2. 99% ) . 67 species Ceratopogonidae of Xinjiang were known, 51 species were recorded in palae-arictic, 16 species of interrelational consists in palaearictic and oriental of Ningxia were recorded. Conclusion Scientific accordance is given for fauna study on Ceratopogonidae of Xinjiang .

  11. Estado actual del conocimiento de la familia Ceratopogonidae en la Patagonia (Diptera: Nematocera Current knowledge of the family Ceratopogonidae in Patagonia (Diptera: Nematocera

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    Gustavo R. Spinelli

    2009-06-01

    Full Text Available Ceratopogonidae incluye pequeños dípteros nematoceros que crían en hábitats acuáticos o semiacuáticos. Hasta los '80, el conocimiento de su taxonomía en la Patagonia se hallaba limitado a la contribución de los comienzos de los '30 de Ingram y Macfie, sobre la base de material capturado en 1926-27, en el área del Parque Nacional Nahuel Huapi y zonas vecinas de Chile. En los últimos 25 años, se pusieron en marcha diferentes proyectos, resultando la descripción o registro de numerosos taxones para la región. Si se contabilizan los datos publicados, 102 especies habitan en la Patagonia, de las cuales 86 (84,31% son endémicas para la región. Este alto porcentaje es evidente en los bosques de Nothofagus, con 71 especies endémicas de este bioma, nueve lo son de la estepa y seis presentan registros en bosque y estepa. Datos aún no publicados concuerdan con este esquema. Diecinueve géneros están representados en la Patagonia, diez de ellos con amplia distribución, tres de distribución transantártica y seis son endémicos para el área. Para la misma, se hallan publicadas las revisiones de Forcipomyia, Atrichopogon, Borkenthelea, Macrurohelea y Paradasyhelea; se han finalizado y aún no publicado aquellas de Dasyhelea y Palpomyia, está muy avanzada la de Stilobezzia y se prevé comenzar con las de Austrohelea, Austrosphaeromias, Physohelea y Bezzia. Se calcula que estos datos no divulgados contienen al menos 55 especies todavía no descriptas. Con respecto a los aspectos biogeográficos se pueden destacar estudios que tienden a establecer relaciones entre las áreas reconocidas en esquemas biogeográficos propuestos para la región andina.Ceratopogonidae includes small nematoceran Diptera which breed in aquatic and semiaquatic habitats. Until the 80´s its taxonomic knowledge in Patagonia was limited to the early 30´s contribution of Ingram and Macfie, from material collected in 1926-27 in the area of the Nahuel Huapi National

  12. Culicoides (Diptera: Ceratopogonidae) host preferences and biting rates in the Netherlands : comparing cattle, sheep and the black-light suction trap

    NARCIS (Netherlands)

    Elbers, A.R.W.; Meiswinkel, R.

    2014-01-01

    Host preference is an important determinant of feeding behaviour in biting insects and a critical component in the transmission of vector-borne diseases. The aim of the study was to quantify Culicoides (Diptera: Ceratopogonidae) host preferences and biting rates using tethered livestock at pasture (

  13. Towards the PCR-based identification of Palaearctic Culicoides biting midges (Diptera: Ceratopogonidae): results from an international ring trial targeting fourspecies of the subgenus Avaritia.

    NARCIS (Netherlands)

    Garros, C.; Balenghien, T.; Carpenter, S.; Delécolle, J.C.; Meiswinkel, R.; Pédarrieu, A.; Rakotoarivony, I.; Gardès, L.; Golding, N.; Barber, J.; Miranda, M.; Borràs Borràs, D.; Goffredo, M.; Monaco, F.; Pagès, N.; Sghaier, S.; Hammami, S.; Calvo, J.H.; Lucientes, J.; Geysen, D.; Deken, de G.; Sarto i Monteys, V.; Schwenkenbecher, J.; Kampen, H.; Hoffmann, B.; Lehman, K.; Werner, D.; Baldet, T.; Lancelot, R.; Cêtre-Sossah, C.

    2014-01-01

    Background Biting midges of the genus Culicoides (Diptera: Ceratopogonidae) are biological vectors of internationally important arboviruses. To understand the role of Culicoides in the transmission of these viruses, it is essential to correctly identify the species involved. Within the western Palae

  14. Biting rates and developmental substrates for biting midges (Diptera: Ceratopogonidae) in Iquitos, Peru.

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    Mercer, David R; Spinelli, Gustavo R; Watts, Douglas M; Tesh, Robert B

    2003-11-01

    Biting midges (Diptera: Ceratopogonidae) were collected at 16 periurban and rural sites around Iquitos, Peru, between 17 October 1996 and 26 May 1997. Culicoides paraensis (Goeldi), the principal vector of Oropouche virus, was the most commonly collected species (9,086 flies) with Culicoides insinuatus Wirth & Blanton second (7,229 flies). Although both species were collected at all sampling sites (linear (distance surveyed approximately 25 km), C. paraensis dominated at northern collection sites (> 90%), whereas C. insinuatus prevailed at southern collection sites (> 60%). C. paraensis were collected from human sentinels at a constant rate throughout daylight hours, at similar rates during wet and dry months, and regardless of rainfall. Larval developmental substrates for C. paraensis included decaying platano (Musa x paradisiaca L. [Musaceae]) stems, stumps, flowers, fruits, and debris beneath platano trees as well as from soil beneath a fruiting mamay (Syzygium malaccense Merr. & Perry [Myrtaceae] ) tree and organic-rich mud along a lake shoreline. C. insinuatus adults likewise emerged from decaying platano and organic-rich mud along a lake shoreline, but also from debris accumulated in the axils of aguaje (Mauritia flexuosa L. [Palmae]) fronds and decaying citrus fruit. Despite high numbers of biting adults near putative substrates, adults of neither species emerged from other decomposing plant material, soil, phytotelmata, or artificial containers. Because both species of biting midges emerged in high numbers from all parts of platano (ubiquitous in Iquitos), it will be challenging to control them through sanitation. PMID:14765657

  15. Occurrence of Culicoides species (Diptera: Ceratopogonidae in the Khomas region of Namibia during the winter months

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    Elbè Becker

    2012-03-01

    Full Text Available Although African horse sickness (AHS is considered to be endemic in Namibia, limited data on its Culicoides midge vector (Diptera: Ceratopogonidae are available. The principal study objective was to determine the presence, species composition and the richness and diversity of Culicoides adults during the colder and drier months in the Khomas region of central Namibia. Five sites were selected, ranging from relatively high to low altitudes with high to low annual rainfall. Onderstepoort suction UV-light traps were used for Culicoides species collection and were run during the winter from 6 July to 21 September 2009. A relatively high diversity of 25 species from 9 091 Culicoides individuals were collected in 34 collections. The abundance of the proven vector of AHS virus, Culicoides imicola, varied from 94% near Windhoek at a high altitude and relatively higher annual rainfall, to 12% at the site situated farthest south-west, with the lowest altitude and annual rainfall. This relatively high Culicoides midge abundance, coupled with the presence of a cycling host (zebra in the area, imply that AHS virus may overwinter in the Khomas region of Namibia.

  16. Notes on the family Ceratopogonidae in Xinjiang%新疆蠓科昆虫小志

    Institute of Scientific and Technical Information of China (English)

    刘增加; 张继军

    2010-01-01

    目的 研究新疆维吾尔自治区双翅目蠓科志.方法 采用帐诱法、网捕法、畜诱法、灯诱法和文献资料收集蠓种.结果 报道了新疆双翅目蠓科昆虫小志,记述了双翅目蠓科(Ceratopogonidae)4亚科10属106种,其中细蠓亚科(Leptoconopinae)1属,即细蠓属(Leptoconops Skuse)17种;毛蠓亚科(Dasyheleinae)1属,即毛蠓属(Dasyhelea Kieffer)16种;铗蠓亚科(Forcipomyiinae)3属,即裸蠓属(Atrichopogon Kieffer)1种,铗蠓属(Forcipomyia Meigen)8种和蠛蠓属(Lasiohelea Kieffer)2种;蠓亚科(Ceratopogoninae)5属,即库蠓属(Culicoides Latreille)48种,短蠓属(Brachypogon Kieffer)4种,贝蠓属(Bezzia Kieffer)8种,须蠓属(Palpomyia Meigen)和塞蠓属(Mallochohelea Wirth)各1种.结论 为新疆双翅目蠓科的进一步研究与防控提供了科学依据.

  17. Biting midges (Diptera: Ceratopogonidae) from Martín García Island, Argentina.

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    Ronderos, María M; Marino, Pablo I; Díaz, Florentina; Estévez, Ana L

    2011-09-01

    Nearly 230 species of biting midges have been recorded or described from Argentina; 38 of them are known from the Buenos Aires province and only one is cited from Martín García Island. This paper presents the results raised from six collecting trips which took place on the island during spring 2005, summer 2006 and autumn 2009. Diverse sampling sites including permanent and temporary aquatic environments were chosen, most of the ten sampling sites were ponds of diverse origin, some of these environments were covered with floating vegetation as Lemna gibba, Lemna minuscule, Salvinia biloba, Salvinia minima, Azolla filiculoides, Limnobium laevigatum, Pistia stratiotes, Spirodela intermedia, Wolffiella oblonga and Wolffia columbiana. Other sites were placed in urban and suburban areas. Adults were collected with sweep nets at sunrise and sunset and with light traps at intervals of four to five hours at night, depending on electricity availability on the island. Larvae and pupae were collected with different implements depending on characteristics of each surveyed aquatic habitat. In free standing water, they were captured with small sieves or hand pipettes and micropipettes, flotation techniques were utilized for sampling vegetated areas, free and rooted floating hydrophytes were extracted for removing insects among them. Thirteen species of Ceratopogonidae were collected, three of Atrichopogon Kieffer, three of Forcipomyia Meigen, two of Dasyhelea Kieffer, four of Culicoides Latreille, and one of Bezzia Kieffer, all representing new records from the island. PMID:22017124

  18. FAMILY CERATOPOGONIDAE.

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    Spinelli, Gustavo A; Wolff, Marta

    2016-01-01

    Two hundred and thirty five species of Ceratopogonids arranged in 28 genera are listed for Colombia, mostly Culicoides (112 species) and Forcipomyia (50 species). For each listed species, information on type locality, type category and sex, location of type material, species distribution, localities for Colombia and main references, is provided. Localities are not specified for 17 species in which the literature searching was negative. Forcipomyia squamitibia Lutz and Bezzia clavipennis are excluded from the list. PMID:27395263

  19. Culicoides spp. (Diptera: Ceratopogonidae) as vectors of bluetongue virus in South Africa - a review.

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    Venter, Gert Johannes

    2015-01-01

    The aim of this paper is to consolidate vector competence studies on Culicoides midges (Diptera: Ceratopogonidae) as vectors of bluetongue virus (BTV) done over a period 25 years at the ARC‑Onderstepoort Veterinary Institute in South Africa. In 1944, it was demonstrated for the first time in South Africa that Culicoides midges transmit BTV. In 1991, field‑collected Culicoides imicola were fed on blood containing BTV‑3 or ‑6 and the infection rates were established as being 31% and 24%, respectively. In 1998, Culicoides bolitinos was shown to have a higher infection prevalence and virus titre/midge than C. imicola. This species was then shown to have a higher transmission potential for BTV‑1 over a range of incubation temperatures wider than the one showed by C. imicola. Attenuation of BTV also does not reduce its ability to infect competent Culicoides species. Oral susceptibility studies, involving 29 BTV isolates of various serotypes, indicated differences between various geographic virus isolates and Culicoides populations evaluated. While low recovery rates of European BTV strains from South African Culicoides species suggest co‑adaptation between orbiviruses and vectors in a given locality, co‑adaption was shown not to be essential for virus transmission. Cumulative results since 1991 provide evidence that at least 13 livestock‑associated Culicoides species are susceptible to BTV. Susceptibility results are supported by field isolations from 5 of these species. This implies that multi‑vector potential for the transmission of BTV will complicate the epidemiology of BT. It must be emphasised that neither oral susceptibility nor virus isolation/detection from field‑collected specimens is proof that a species is a confirmed field vector. PMID:26741247

  20. Haemoproteus tartakovskyi (Haemoproteidae): Complete sporogony in Culicoides nubeculosus (Ceratopogonidae), with implications for avian haemoproteid experimental research.

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    Žiegytė, Rita; Bernotienė, Rasa; Palinauskas, Vaidas; Valkiūnas, Gediminas

    2016-01-01

    Numerous recent studies have addressed the molecular characterization, distribution and genetic diversity of Haemoproteus spp. (Haemoproteidae). Some species of these blood parasites cause severe disease in birds, and heavy infections are often lethal in biting midges (Ceratopogonidae) and other blood-sucking insects. However, information about the vectors of haemoproteids is scarce. This presents an obstacle for better understanding the mechanisms of host-parasite interactions and the epidemiology of haemoproteosis. Here we investigated the sporogonic development of Haemoproteus tartakovskyi, a widespread bird parasite, in experimentally infected biting midges, Culicoides nubeculosus. These biting midges are widespread in the Europe. The insects were cultivated under laboratory conditions. Unfed females were allowed to take blood meals on wild caught siskins Carduelis spinus naturally infected with H. tartakovskyi (lineage hSISKIN1). Engorged females were maintained at 22-23 °C, dissected at intervals, and examined for sporogonic stages. Mature ookinetes of H. tartakovskyi were seen in the midgut content between 6 and 48 h post infection, oocysts were observed in the midgut wall 3-4 days post infection (dpi). Sporozoites were first reported in the salivary gland preparations 7 dpi. In accordance with microscopy data, polymerase chain reaction amplification and sequencing confirmed presence of the corresponding parasite lineage in experimentally infected biting midges. This study indicates that C. nubeculosus willingly takes blood meals on birds and is a vector of H. tartakovskyi. These biting midges are readily amenable to cultivation under laboratory conditions. Culicoides nubeculosus transmits Haemoproteus parasites infecting parrots, owls and siskins, birds belonging to different families and orders. Thus, this vector provides a convenient model for experimental research with avian haemoproteids. PMID:26616347

  1. Lista dos exemplares tipos de Ceratopogonidae (Diptera: Nematocera depositados na Coleção Entomológica do Instituto Oswaldo Cruz, Rio de Janeiro, Brasil List of the type species of Ceratopogonidae (Diptera, Nematocera deposited in the Entomological Collection of Instituto Oswaldo Cruz, Rio de Janeiro, Brazil

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    ML Felippe-Bauer

    2001-11-01

    Full Text Available A list of all type specimens of the Family Ceratopogonidae, present in the Entomological Collection of Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brazil is presented. This list includes the genera Bahiahelea, Culicoides, Dasyhelea, Downeshelea, Forcipomyia, Leptoconops, Mallochohelea, Monohelea, Neobezzia, Palpomyia and Sphaerohelea.

  2. Delineation of Culicoides species by morphology and barcode exemplified by three new species of the subgenus Culicoides (Diptera: Ceratopogonidae) from Scandinavia

    OpenAIRE

    Nielsen, Søren Achim; Kristensen, Michael

    2015-01-01

    Background Culicoides biting midges (Diptera: Ceratopogonidae) cause biting nuisance to livestock and humans and are vectors of a range of pathogens of medical and veterinary importance. Despite their economic significance, the delineation and identification of species where only morphology is considered, as well as the evolutionary relationships between species within this genus remains problematic. In recent years molecular barcoding has assisted substantially in the identification of bitin...

  3. Les porcheries : réservoirs des Culicoides (Diptera : Ceratopogonidae), vecteurs des virus de la Maladie de la Langue bleue et de Schmallenberg ?

    OpenAIRE

    Zimmer, JY.; Saegerman, C; Martinelle, L.; Losson, B.; Leroy, P.; Haubruge, E.; Francis, F.

    2014-01-01

    Pig farms: reservoirs of vectors of Bluetongue and Schmallenberg viruses?. Bluetongue (BT) is a vector-borne disease that affects domestic and wild ruminants. Since its recent outbreak in northern Europe, this viral disease has caused considerable economic losses. The biological vectors of the bluetongue virus are biting midges belonging to the genus Culicoides (Diptera: Ceratopogonidae). Several light trapping campaigns targeting these adult midges have been previously conducted in Belgium w...

  4. Vector competence of Culicoides sonorensis (Diptera: Ceratopogonidae to epizootic hemorrhagic disease virus serotype 7

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    Ruder Mark G

    2012-10-01

    Full Text Available Abstract Background Culicoides sonorensis (Diptera: Ceratopogonidae is a vector of epizootic hemorrhagic disease virus (EHDV serotypes 1 and 2 in North America, where these viruses are well-known pathogens of white-tailed deer (WTD and other wild ruminants. Although historically rare, reports of clinical EHDV infection in cattle have increased in some parts of the world over the past decade. In 2006, an EHDV-7 epizootic in cattle resulted in economic loss for the Israeli dairy industry. White-tailed deer are susceptible to EHDV-7 infection and disease; however, this serotype is exotic to the US and the susceptibility of C. sonorensis to this cattle-virulent EHDV is not known. The objective of the study was to determine if C. sonorensis is susceptible to EHDV-7 infection and is a competent vector. Methods To evaluate the susceptibility of C. sonorensis, midges were fed on EHDV-7 infected WTD, held at 22 ± 1°C, and processed individually for virus isolation and titration on 4–16 days post feeding (dpf. Midges with a virus titer of ≥102.7 median tissue culture infective doses (TCID50/midge were considered potentially competent. To determine if infected C. sonorensis were capable of transmitting EHDV-7 to a host, a susceptible WTD was then fed on by a group of 14–16 dpf midges. Results From 4–16 dpf, 45% (156/350 of midges that fed on WTD with high titer viremia (>107 TCID50/ml were virus isolation-positive, and starting from 10–16 dpf, 32% (35/109 of these virus isolation-positive midges were potentially competent (≥102.7 TCID50/midge. Midges that fed on infected deer transmitted the virus to a susceptible WTD at 14–16 dpf. The WTD developed viremia and severe clinical disease. Conclusion This study demonstrates that C. sonorensis is susceptible to EHDV-7 infection and can transmit the virus to susceptible WTD, thus, C. sonorensis should be considered a potential vector of EHDV-7. Together with previous work, this study demonstrates

  5. Actualización del catálogo de Culicoides Latreille, 1809 (Diptera, Ceratopogonidae de España

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    Lucientes, J.

    2012-12-01

    Full Text Available The number of studies on arthropods of genus Culicoides Latreille (Diptera, Ceratopogonidae has increased considerably in Spain in recent decades. This is due to the role these insects play as vectors of arboviruses that cause disease in animals, but also in humans. This work undertakes an updated catalogue of the species of this genus in our country, through a critical review of the literature, clarifying chronological aspects of these publications carried out for over a century of research. This update shows a total of 81 species of Culicoides in Spain, among which are some to be considered as directly related to the transmission of diseases such as bluetongue and African horse sickness.El número de estudios acerca de los artrópodos del género Culicoides Latreille (Diptera, Ceratopogonidae en España ha experimentado un elevado incremento en las últimas décadas. Principalmente ello es debido al papel que estos dípteros juegan como vectores de arbovirus causantes de enfermedades en los animales, aunque también en humanos. Este trabajo acomete una actualización del catálogo de las especies que conforman este género en nuestro país, mediante una revisión crítica de la literatura existente, clarificando aspectos cronológicos sobre estas publicaciones llevadas a cabo durante más de un siglo de investigación. Esta actualización muestra un total de 81 especies de Culicoides para España, entre las que se encuentran algunas a tener en cuenta por estar directamente relacionadas con la trasmisión de enfermedades como la Lengua Azul o la Peste Equina Africana.

  6. PRELIMINARY REPORT OF SURVEY OF CERATOPOGONIDAE ON LAUTAU ISLAND, HONG KONG (DIPTERA, CERATOPOGONIDAE)%香港大屿山岛蠓科调查初报(双翅目,蠓科)

    Institute of Scientific and Technical Information of China (English)

    李明伟; 张家润; 梁志华; 霍明茵; 赖玉龙; 虞以新

    2008-01-01

    Eleven species from 5 genera belonging to 3 subfamilies of Ceratopogonidae were collected on Lautau Island in the first survey. Among them, two are new species and three are new record species in China, while the rest are recorded in the Hong Kong Special Administrative Region for the first time. This paper describes one of the two new species, Brachypogon halimos Yu et Li, sp. nov. The other has already been reported in another paper.%2006年5月在大屿山岛初次调查,采获一批蠓科昆虫,经分类鉴定共有蠓类3亚科5属11种,其中有1个新种,3个中国新纪录,其余蠓种除绿腹柱蠓Stilobezzia chlorogastrula Yu & Yuan外,都是香港特区的首次记录.描述了滨海短蠓Brachypogon halimos Yu et Li,sp.nov.,同时对环基库蠓Culicoides circumbasalis Tokunaga、短唇铗蠓Forcipomyia(Microhelea)brevilabellata和浅淡铗蠓Forcipomyia(Warmea)lesliei种中国3新纪录种做了简单的记述.

  7. Chemical composition of silage residues sustaining the larval development of the Culicoides obsoletus/Culicoides scoticus species (Diptera: Ceratopogonidae).

    Science.gov (United States)

    Zimmer, Jean-Yves; Saegerman, Claude; Losson, Bertrand; Beckers, Yves; Haubruge, Eric; Francis, Frédéric

    2013-01-16

    Culicoides (Diptera: Ceratopogonidae) are biological vectors of bluetongue virus (BTV). Bluetongue is a viral disease that affects domestic and wild ruminants. Since its recent emergence in northern Europe, this disease has caused considerable economic losses to the sheep and cattle industry. The biotopes, and more particularly the chemical characteristics which are suitable for larval development of the main vector species, are still relatively unknown. This study shows that the larvae of biting midges belonging to the species Culicoides obsoletus and Culicoides scoticus are able to breed in different types of silage residue (maize, grass, sugar beet pulp and their combinations). The chemical composition of substrates strongly influences the presence of the immature stages of these biting midges. Higher lignin and insoluble fibre contents seem to favour their presence and could play the role of a physical support for semi-aquatic larvae. In contrast, higher concentrations of magnesium and calcium are negatively correlated with the presence of these two species. These data will help to locate and monitor the breeding sites of these species and could contribute to the control of these insects on farms.

  8. Chemical composition of silage residues sustaining the larval development of the Culicoides obsoletus/Culicoides scoticus species (Diptera: Ceratopogonidae).

    Science.gov (United States)

    Zimmer, Jean-Yves; Saegerman, Claude; Losson, Bertrand; Beckers, Yves; Haubruge, Eric; Francis, Frédéric

    2013-01-16

    Culicoides (Diptera: Ceratopogonidae) are biological vectors of bluetongue virus (BTV). Bluetongue is a viral disease that affects domestic and wild ruminants. Since its recent emergence in northern Europe, this disease has caused considerable economic losses to the sheep and cattle industry. The biotopes, and more particularly the chemical characteristics which are suitable for larval development of the main vector species, are still relatively unknown. This study shows that the larvae of biting midges belonging to the species Culicoides obsoletus and Culicoides scoticus are able to breed in different types of silage residue (maize, grass, sugar beet pulp and their combinations). The chemical composition of substrates strongly influences the presence of the immature stages of these biting midges. Higher lignin and insoluble fibre contents seem to favour their presence and could play the role of a physical support for semi-aquatic larvae. In contrast, higher concentrations of magnesium and calcium are negatively correlated with the presence of these two species. These data will help to locate and monitor the breeding sites of these species and could contribute to the control of these insects on farms. PMID:22963713

  9. First report of 13 species of Culicoides (Diptera: Ceratopogonidae in mainland Portugal and Azores by morphological and molecular characterization.

    Directory of Open Access Journals (Sweden)

    David W Ramilo

    Full Text Available The genus Culicoides (Diptera: Ceratopogonidae contains important vectors of animal and human diseases, including bluetongue, African horse sickness and filariosis. A major outbreak of bluetongue occurred in mainland Portugal in 2004, forty eight years after the last recorded case. A national Entomological Surveillance Plan was initiated in mainland Portugal, Azores and the Madeira archipelagos in 2005 in order to better understand the disease and facilitate policy decisions. During the survey, the most prevalent Culicoides species in mainland Portugal was C. imicola (75.3% and species belonging to the Obsoletus group (6.5%. The latter were the most prevalent in Azores archipelago, accounting for 96.7% of the total species identified. The Obsoletus group was further characterized by multiplex Polymerase Chain Reaction to species level showing that only two species of this group were present: C. obsoletus sensu strictu (69.6% and C. scoticus (30.4%. Nine species of Culicoides were detected for the first time in mainland Portugal: C. alazanicus, C. bahrainensis, C. deltus, C. lupicaris, C. picturatus, C. santonicus, C. semimaculatus, C. simulator and C. subfagineus. In the Azores, C. newsteadi and C. circumscriptus were identified for the first time from some islands, and bluetongue vectors belonging to the Obsoletus group (C. obsoletus and C. scoticus were found to be widespread.

  10. Les porcheries : réservoirs des Culicoides (Diptera : Ceratopogonidae, vecteurs des virus de la Maladie de la Langue bleue et de Schmallenberg ?

    Directory of Open Access Journals (Sweden)

    Zimmer, JY.

    2014-01-01

    Full Text Available Pig farms: reservoirs of vectors of Bluetongue and Schmallenberg viruses?. Bluetongue (BT is a vector-borne disease that affects domestic and wild ruminants. Since its recent outbreak in northern Europe, this viral disease has caused considerable economic losses. The biological vectors of the bluetongue virus are biting midges belonging to the genus Culicoides (Diptera: Ceratopogonidae. Several light trapping campaigns targeting these adult midges have been previously conducted in Belgium within cattle and sheep farms, but none have been performed inside pig farms. This study therefore aims to assess, using light traps, the levels of Culicoides populations that may have been present inside two Belgian pig farms during the fall and winter of 2008. The presence of (potential Culicoides vector species was demonstrated inside the pig buildings during the fall: 8 and 749 specimens belonging to 2 and 7 species were respectively trapped inside the pigsties, with the majority being Obsoletus complex females. The opening up of the buildings seemed to strongly influence their presence. Observation of the females' nutritional status suggests that these midges were likely to have fed or to have laid eggs inside the pig farms, despite the fact that pig's blood could not be identified in the abdomen of engorged females and that pig manure did not reveal the presence of larvae. Pigs could thus be involved in the maintenance of potential vector species populations of the BT virus, or of the new Schmallenberg virus.

  11. First record of Culicoides oxystoma Kieffer and diversity of species within the Schultzei group of Culicoides Latreille (Diptera: Ceratopogonidae biting midges in Senegal.

    Directory of Open Access Journals (Sweden)

    Mame T Bakhoum

    Full Text Available The Schultzei group of Culicoides Latreille (Diptera: Ceratopogonidae is distributed throughout Africa to northern Asia and Australasia and includes several potential vector species of livestock pathogens. The taxonomy of the species belonging to this species group is confounded by the wide geographical distribution and morphological variation exhibited by many species. In this work, morphological and molecular approaches were combined to assess the taxonomic validity of the species and morphological variants of the Schultzei group found in Senegal by comparing their genetic diversity with that of specimens from other geographical regions. The species list for Senegal was updated with four species: Culicoides kingi, C. oxystoma, C. enderleini and C. nevilli being recorded. This is the first record of C. oxystoma from Africa south of Sahara, and its genetic relationship with samples from Israel, Japan and Australia is presented. This work provides a basis for ecological studies of the seasonal and spatial dynamics of species of this species group that will contribute to better understanding of the epidemiology of the viruses they transmit.

  12. Culicoides (Diptera: Ceratopogonidae) and livestock in the Netherlands: comparing host preference and attack rates on a Shetland pony, a dairy cow, and a sheep.

    Science.gov (United States)

    Elbers, A R W; Meiswinkel, R

    2015-12-01

    Culicoides (Diptera: Ceratopogonidae) host preferences and attack rates were quantified in early summer at a dairy farm in the Netherlands using livestock tethered at pasture. Midges were aspirated hourly over seven consecutive hours (17:00-23:00) from a dairy cow, a Shetland pony, and a sheep and correspondingly yielded seventeen, thirteen, and nine species. Of the 14,181 midges obtained, approximately 95% belonged to the C. obsoletus complex, C. dewulfi, C. chiopterus, and C. punctatus that together include all proven or potential vectors for arboviral diseases in livestock in northwestern Europe. On average, 7.6 and 3.5 times more Culicoides were collected, respectively, from the cow and the Shetland pony than from the sheep. In descending order of abundance, the C. obsoletus complex, C. dewulfi, and C. chiopterus dominated attacks on all three hosts, whereas C. punctatus and C. pulicaris favored only the two larger hosts. Irrespective of the host species involved, the three body regions attracted the same component species, C. chiopterus favoring the legs, C. punctatus and C. achrayi the belly, and the C. obsoletus complex, C. dewulfi, and C. pulicaris the head, back, and flanks. That known and potential vectors for animal diseases feed indiscriminately on a broad range of mammal hosts means that all major livestock species, including equines, are rendered susceptible to one or more Culicoides-borne pathogens. PMID:26611966

  13. Seasonal Dynamics, Parity Rate, and Composition of Culicoides (Diptera: Ceratopogonidae) Occurring in the Vicinity of Wild and Domestic Ruminants in the Czech Republic.

    Science.gov (United States)

    Rádrová, Jana; Mračková, Marie; Galková, Zdenka; Lamka, Jírí; Račka, Karol; Barták, Pavel; Votýpka, Jan

    2016-03-01

    In the light of the emergence of bluetongue and Schmallenberg viruses in northern and central Europe, an extensive entomological survey within the framework of a bluetongue control program was undertaken from 2008 to 2013 in the Czech Republic to investigate Culicoides biting midges (Diptera: Ceratopogonidae) collected in close proximity of domestic livestock and semiwild ruminants. Insects were sampled using CDC black-light suction traps placed overnight near ruminants in farms or in forest game preserves to provide data on Culicoides fauna collected near these two groups of hosts inhabiting different environments. From almost a half million biting midge specimens collected at 41 sampling sites, 34 species were identified including three species newly recorded for the Czech Republic: Culicoides (Oecacta) clastrieri Callot, Kremer & Deduit, Culicoides (Oecacta) odiatus Austen, and Culicoides (Pontoculicoides) saevus Kieffer. The Culicoides obsoletus species group, incriminated as a bluetongue virus vector, was predominant in both domestic livestock (91%) and semiwild game (52%). A relatively high proportion (around 30%) of C. obsoletus Meigen females with pigmented abdomen (= more likely parous) was observed from spring till autumn. In contrast, adult biting midges were found to be largely absent during at least three winter months, approximately December till March, which could be considered as the biting midge vector-free period. PMID:26701798

  14. Assessment of vector/host contact: comparison of animal-baited traps and UV-light/suction trap for collecting Culicoides biting midges (Diptera: Ceratopogonidae, vectors of Orbiviruses

    Directory of Open Access Journals (Sweden)

    Delécolle Jean-Claude

    2011-06-01

    Full Text Available Abstract Background The emergence and massive spread of bluetongue in Western Europe during 2006-2008 had disastrous consequences for sheep and cattle production and confirmed the ability of Palaearctic Culicoides (Diptera: Ceratopogonidae to transmit the virus. Some aspects of Culicoides ecology, especially host-seeking and feeding behaviors, remain insufficiently described due to the difficulty of collecting them directly on a bait animal, the most reliable method to evaluate biting rates. Our aim was to compare typical animal-baited traps (drop trap and direct aspiration to both a new sticky cover trap and a UV-light/suction trap (the most commonly used method to collect Culicoides. Methods/results Collections were made from 1.45 hours before sunset to 1.45 hours after sunset in June/July 2009 at an experimental sheep farm (INRA, Nouzilly, Western France, with 3 replicates of a 4 sites × 4 traps randomized Latin square using one sheep per site. Collected Culicoides individuals were sorted morphologically to species, sex and physiological stages for females. Sibling species were identified using a molecular assay. A total of 534 Culicoides belonging to 17 species was collected. Abundance was maximal in the drop trap (232 females and 4 males from 10 species whereas the diversity was the highest in the UV-light/suction trap (136 females and 5 males from 15 species. Significant between-trap differences abundance and parity rates were observed. Conclusions Only the direct aspiration collected exclusively host-seeking females, despite a concern that human manipulation may influence estimation of the biting rate. The sticky cover trap assessed accurately the biting rate of abundant species even if it might act as an interception trap. The drop trap collected the highest abundance of Culicoides and may have caught individuals not attracted by sheep but by its structure. Finally, abundances obtained using the UV-light/suction trap did not estimate

  15. Catálogo das coleções entomológicas da Faculdade de Saúde Pública da Universidade de São Paulo (1.a série: Ceratopogonidae, Psychodidae, Simuliidae

    Directory of Open Access Journals (Sweden)

    Oswaldo Paulo Forattini

    1971-12-01

    Full Text Available Família CERATOPOGONIDAE: Gênero Alluaudomyia Kieffer, 1913; Gênero Atrichopogon Kieffer, 1906; Gênero Bezzia Kieffer, 1899; Gênero Calyptopogon Kieffer, 1910; Gênero Ceratobezzia Kieffer, 1917; Gênero Clinohelea Kieffer, 1917; Gênero Culicoides Latreille, 1809; Gênero Dasyhelea Kieffer, 1911; Gênero Dicrobezzia Kieffer, 1919; Gênero Dicrohele Lane & Wirth, 1961; Gênero Echinohele Macfie, 1942; Gênero Forcipomya Meigen, 1818; Gênero Heteromyia Say, 1825; Gênero Lasiohelea (Kieffer, 1921; Gênero Leptocanops Skuse, 1889; Gênero Macfiehelea Lane, 1946; Gênero Macropeza Meigen, 1818; Gênero Monohelea Kieffer, 1917; Gênero Nilobezzia Kieffer, 1921; Gênero Pachyhelea Wirth, 1959; Gênero Palpomyia Meigen, 1818; Gênero Parabezzia Malloch, 1915; Gênero Paryphoconus Enderlein, 1912; Gênero Sphaeromias Curtis, 1829; Gênero Stenoxenus Coquillett, 1899; Gênero Stilobezzia Kieffer, 1911; Familia PSYCHODIDAE: Gênero Bruchomyia Alexander, 1920; Gênero Phlebotomus Rondani, 1840; Família SIMULIIDAE: Gênero Eusimulium Roubaud, 1906; Gênero Lutzsimulium d'Andretta & d'Andretta, 1947; Gênero Prosimulium Roubaud, 1906; Gênero Simulium Latreille, 1802.

  16. Larval development sites of the main Culicoides species (Diptera: Ceratopogonidae) in northern Europe and distribution of coprophilic species larvae in Belgian pastures.

    Science.gov (United States)

    Zimmer, Jean-Yves; Brostaux, Yves; Haubruge, Eric; Francis, Frédéric

    2014-10-15

    Some Culicoides species of biting midges (Diptera: Ceratopogonidae) are biological virus vectors worldwide and have indeed been associated with outbreaks of important epizoonoses in recent years, such as bluetongue and Schmallenberg disease in northern Europe. These diseases, which affect domestic and wild ruminants, have caused considerable economic losses. Knowledge of substrates suitable for Culicoides larval development is important, particularly for the main vector temperate species. This study, realized during two years, aimed to highlight the larval development sites of these biting midge species in the immediate surroundings of ten Belgian cattle farms. Moreover, spatial distribution of the coprophilic Culicoides larvae (C. chiopterus and C. dewulfi) within pastures was studied with increasing distance from farms along linear transects (farm-pasture-woodland). A total of 4347 adult specimens belonging to 13 Culicoides species were obtained by incubation of 2131 soil samples belonging to 102 different substrates; 18 of these substrates were suitable for larval development. The Obsoletus complex (formed by two species) was observed in a wide range of substrates, including silage residues, components of a chicken coop, dung adhering to walls inside stables, leftover feed along the feed bunk, a compost pile of sugar beet residues, soil of a livestock trampling area, and decaying wood, while the following served as substrates for the other specimens: C. chiopterus, mainly cow dung; C. dewulfi, cow dung and molehill soil; C. circumscriptus, algae; C. festivipennis, algae and soil in stagnant water; C. nubeculosus, algae and silt specifically from the edge of a pond; C. punctatus, mainly wet soil between silage reserves; C. salinarius, algae; and C. stigma, algae and wet soil between silage reserves. We also recorded significantly higher densities of coprophilic larvae within pastures in cow dung located near forests, which is likely due to the localization of

  17. Catalogue and a new species of family Ceratopogonidae from Heilongjiang province in China%黑龙江省蠓科小志及一新种(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    刘国平; 邢安辉; 任清明; 王旭; 王峰

    2011-01-01

    Objective To determine the species distribution of Ceratopogonidae from Heilongjiang province. Methods Biting midges were collected using human-baited net traps, light traps, animal-baited traps and insect nets to generate data on the species distribution in Heilongjiang province. Results A total of 421 000 biting midges were collected, which belonged to 3 subfamilies, 13 genera and 89 species, including one new species recorded Culicoides heilongjiangensis Liu et Ren sp. Nov. The subfamily Dasyheleinae belonging to one genus and 5 species, subfamily Forcipomyiinae belonging to 3 genera and 23 species, subfamily Ceratopogoninae belonging to 9 genera and 61 species, and bloodsucking midges of 2 genera and 47 species were recorded. The distribution of 89 known species of biting midges in various cities of Heilongjiang was recorded. Conclusion The investigation informed the faunal distribution of Ceratopogonidae in Heilongjiang, China.C. Heilongjiangensis Liu et Ren sp. Nov. Is alike to C. Duddingstoni Kettle et Lawson, 1955 in the wing spots, but the latter is noticeably distinguishable by shape of male hypopygium. Shape of male hypopygium of this new species is also alike to C. Poperinghensis Coetghebuer, 1960, but the latter is distinctly characterized by shapes of ninth sternum, ventral root of basistyle, and distal portion of aedeagus. The type specimen is stored at the Shenyang Command Center for Disease Control and Prevention, Shenyang 110034, Liaoning Province, China%目的 调查黑龙江省蠓科昆虫的种类分布.方法 采用人帐诱、诱虫灯诱、动物诱和网捕法采集蠓类;参考相关文献.结果 在黑龙江省采获蠓科昆虫421 000只,计3亚科13属89种,其中包括一新种:黑龙江库蠓(Culicoides heilongiangensis Liu et Ben sp.nov.);毛蠓亚科1属5种,铗蠓亚科3属23种,蠓亚科9属61种;吸血蠓2属47种.记述了黑龙江省蠓科已知89种的市、县分布.结论 为黑龙江省蠓科昆虫的区系分布研

  18. Mosquito repellent attracts Culicoides imicola (Diptera: Ceratopogonidae).

    Science.gov (United States)

    Braverman, Y; Chizov-Ginzburg, A; Mullens, B A

    1999-01-01

    A plant-derived mosquito repellent, based on the oil of Eucalyptus maculata var. citriodora Hook, was evaluated against the biting midge Culicoides imicola Kieffer. Suction black light-traps covered with repellent-impregnated polyester mesh and deployed near horses attracted large numbers of C. imicola, which were seen near the treated net within a few minutes of the start of the experiment. Initial collections in the traps were approximately 3 times as large as those in control traps with untreated mesh. Numbers collected in treated traps were similar to untreated control traps after 4 h. Traps with mesh treated with DEET or another plant-derived (Meliaceae) proprietary product, AG1000, acted as repellents relative to the control. The differential activity of repellents against blood-feeding Diptera is discussed. PMID:10071502

  19. Hyaluronidase Activity in Saliva of European Culicoides (Diptera: Ceratopogonidae)

    OpenAIRE

    Vlkova, Michaela; Volfova, Vera; Sumova, Petra; Cetre-Sossah, Catherine; Carpenter, Simon; Darpel, Karin; Rakotoarivony, Ignace; Allene, Xavier; Votypka, Jan; Volf, Petr

    2015-01-01

    Biting midges of the genus Culicoides transmit pathogens of veterinary importance such as bluetongue virus (Reoviridae: Orbivirus). The saliva of Culicoides is known to contain bioactive molecules including peptides and proteins with vasodilatory and immunomodulative properties. In this study, we detected activity of enzyme hyaluronidase in six Culicoides species that commonly occur in Europe and that are putative vectors of arboviruses. Hyaluronidase was present in all species studied, altho...

  20. The bloodsucking biting midges of Argentina (Diptera: Ceratopogonidae

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    Gustavo R Spinelli

    2005-04-01

    Full Text Available A key is presented for the identification of the adults of 54 species of bloodsucking ceratopogonids, 51 of which are known inhabitants of Argentina, and Culicoides uruguayensis Ronderos, C. pifanoi Ortiz, and C. trilineatus Fox, which are known to occur in bordering Uruguay and Paraguay. Wing photographs are provided of females of the 45 species of Culicoides. Three new species of Culicoides Latreille from Northeastern Argentina are described and illustrated: C. austroparaensis Spinelli, C. bachmanni Spinelli, and C. williamsi Spinelli. The following six species are recorded for the first time from Argentina and/or bordering localities in Paraguay: Leptoconops brasiliensis (Lutz, C. gabaldoni Ortiz, C. ginesi Ortiz, C. pifanoi Ortiz, C. pseudocrescentis Tavares and Luna Dias, and C. trilineatus; and C. estevezae Ronderos and Spinelli is newly recorded from Misiones province of Argentina. C. lopesi Barretto is excluded from the Argentinean ceratopogonid fauna.

  1. Description of the male of Culicoides pampaensis (Diptera: Ceratopogonidae

    Directory of Open Access Journals (Sweden)

    Gustavo R. SPINELLI

    2005-01-01

    Full Text Available Se describe e ilustra el macho de Culicoides pampaensis, y se brinda una diagnosis de la hembra. El ejemplar descripto fue capturado a la luz junto a una hembra, en la provincia de Catamarca, Argentina. La genitalia se compara con la de Culicoides bambusicola Lutz.

  2. Biting Midges (Ceratopogonidae: Culicoides Latreille) Recorded from Farms in Sweden

    DEFF Research Database (Denmark)

    Nielsen, S. A.; Nielsen, B.O.; Chirico, J.

    2009-01-01

    In light of the emergence of bluetongue in Northern Europe, populations of Culicoides species were monitored in and around several Swedish livestock farms (surveillance in 2007 and 2008). The position of the sampling sites ranged from about latitude 55° N to about 68° N. Thirty-three Culicoides...... species were recorded, of which 30 were new to Sweden. The species recorded, and their relative abundance and spatial distribution on sites are detailed. Species incriminated as vectors of bluetongue virus were predominant. (Texte intégral)...

  3. Culicoides midges (Diptera: Ceratopogonidae as vectors of orbiviruses in Slovakia

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    Adela Sarvašová

    2014-09-01

    Full Text Available In recent years, rapid spread of Culicoides-borne pathogens such as bluetongue (BT and Schmallenberg viruses have been reported in Europe. In this study we examined the Culicoides populations in farms with wild and domestic ruminants in Eastern Slovakia with the aim to confirm the presence of biting midges serving as potential vectors of important pathogens. The main vector complexes were the Obsoletus complex (54%; n=4,209 and the Pulicaris complex (23%; n=1,796. To estimate the relative abundance of the cryptic species of the Obsoletus complex (Culicoides obsoletus, Culicoides scoticus and Culicoides montanus, we performed the multiplex polymerase chain reaction (PCR based on ITS-2 and ITS-1 segments, on 125 midges randomly sampled. The relative abundance of C. obsoletus ranged from 5.26% in the farm with wild ruminants to 85.71% in another farm with cattle and sheep. A total of 112 pools of parous and gravid females belonging to the Obsoletus and Pulicaris complexes were tested for virus detection by the real-time reverse transcription polymerase chain reaction (RT-PCR for BT virus, as well as for the Epizootic Hemorrhagic Disease Virus (EHDV, with negative results.

  4. Description of the male of Culicoides dureti (Diptera: Ceratopogonidae Descripción del macho de Culicoides dureti (Diptera: Ceratopogonidae

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    Gustavo R. Spinelli

    2007-12-01

    Full Text Available The male of Culicoides dureti Ronderos & Spinelli is described and illustrated from specimens collected associated with females in the province of Misiones, Argentina, and a brief description of the female is provided. The species is compared with Culicoides barbosai Wirth & Blanton.Se describe e ilustra el macho de Culicoides dureti Ronderos & Spinelli y se brinda una breve descripción de la hembra sobre la base de ejemplares capturados, asociados con hembras en la Provincia de Misiones, Argentina. La especie es comparada con Culicoides barbosai Wirth & Blanton.

  5. Description of the male of Culicoides dureti (Diptera: Ceratopogonidae) Descripción del macho de Culicoides dureti (Diptera: Ceratopogonidae)

    OpenAIRE

    Gustavo R. Spinelli; Ronderos, María M.

    2007-01-01

    The male of Culicoides dureti Ronderos & Spinelli is described and illustrated from specimens collected associated with females in the province of Misiones, Argentina, and a brief description of the female is provided. The species is compared with Culicoides barbosai Wirth & Blanton.Se describe e ilustra el macho de Culicoides dureti Ronderos & Spinelli y se brinda una breve descripción de la hembra sobre la base de ejemplares capturados, asociados con hembras en la Provincia de Misiones, Arg...

  6. Morphological alterations in Neotropical Ceratopogonidae (Diptera Alterações morfológicas em Ceratopogonidae (Diptera Neotropicais

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    Maria L. Felippe-Bauer

    2006-09-01

    Full Text Available Morphological alterations in six different species of females Culicoides Latreille, 1809 and one of Monohelea Kieffer, 1917 from Brazil, Mexico, Panama and Peru are described. The correlation of the morphological changes with the taxonomy and behavior of the species is discussed.São descritas as alterações morfológicas em fêmeas de seis espécies de Culicoides Latreille, 1809 e uma de Monohelea Kieffer, 1917 provenientes do Brasil, México, Panamá e Peru. É discutida a correlação das alterações morfológicas com a taxonomia e as atividades das espécies.

  7. Descripción del macho de Culicoides pampaensis (Diptera: Ceratopogonidae) Description of the male of Culicoides pampaensis (Diptera: Ceratopogonidae)

    OpenAIRE

    Gustavo R. Spinelli; Ronderos, María M.

    2005-01-01

    RESUMEN. Se describe e ilustra el macho de Culicoides pampaensis, y se brinda una diagnosis de la hembra. El ejemplar descripto fue capturado a la luz junto a una hembra, en la provincia de Catamarca, Argentina. La genitalia se compara con la de Culicoides bambusicola Lutz.ABSTRACT. The male of Culicoides pampaensis Spinelli & Wirth is described and illustrated and female diagnosis is also provided. The described male specimen was captured at light, associated with a female, in Catamarca prov...

  8. A new Culicoides of the stigmalis group (Diptera: Ceratopogonidae Um novo Culicoides do grupo stigmalis (Diptera: Ceratopogonidae

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    Maria Luiza Felippe-Bauer

    1987-09-01

    Full Text Available Notes are presented on the three known Neotropical species of the Culicoides stigmalis group of boodsucking midges: alvarezi Ortiz, fluviatilis (Lutz, stigmalis Wirth, and on deanei n.sp., which is described from the state of Rio de Janeiro, Brazil. A diagnosis is given for the group, as well as a key for identification and comparative phtographs of the female wings.São apresentadas notas sobre as três espécies conhecidas de Culicoides neotropicais do grupo stigmalis: alvarezi Ortiz, fluviatilis (Lutz, stigmalis Wirth e sobre deanei sp.n., a qual é descrita para o Estado do Rio de Janeiro, Brasil. São apresentadas diagnose do grupo, chave sistemática para a identificação e fotografias das asas das fêmeas das espécies do grupo.

  9. Los Ceratopogonidae (Diptera: Culicomorpha del Sistema Serrano de Ventania (Buenos Aires, Argentina The Ceratopogonidae (Diptera: Culicomorphafrom the Sistema Serrano de Ventania (Buenos Aires, Argentina

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    Pablo I. Marino

    2011-12-01

    Full Text Available Se brinda un listado de especies de ceratopogónidos presentes en el Sistema Serrano de Ventania, Buenos Aires, Argentina. Se realizaron seis viajes de estudio y se relevaron nueve sitios de muestreo. Se identificaron 25 especies pertenecientes a ocho géneros, de las cuales sólo Culicoides venezuelensis y Brachypogon (Brachypogon bonaerensis han sido citadas previamente para el área. Ocho especies se registran por primera vez para la provincia de Buenos Aires y una para Argentina.A list of the ceratopogonid species from the Sistema Serrano de Ventania, Buenos Aires, Argentina is presented. Six field trips were carried out sampling nine different localities. Twenty five species of ceratopogonids belonging to eight genera were recorded; only Culicoides venezuelensis and Brachypogon (Brachypogon bonaerensis were known from the area. Eight species are reported for the first time from Buenos Aires province and one from Argentina.

  10. Identity and diversity of blood meal hosts of biting midges (Dipterea: Ceratopogonidae: Culicoides Latreille) in Denmark

    DEFF Research Database (Denmark)

    Lassen, Sandra; Nielsen, Søren Achim; Kristensen, Michael

    2012-01-01

    BACKGROUND: Host preference studies in haematophagous insects e.g. Culicoides biting midges are pivotal to assess transmission routes of vector-borne diseases and critical for the development of veterinary contingency plans to identify which species should be included due to their risk potential....... Species of Culicoides have been found in almost all parts of the world and known to live in a variety of habitats. Several parasites and viruses are transmitted by Culicoides biting midges including Bluetongue virus and Schmallenberg virus. The aim of the present study was to determine the identity and...... the species of the collected biting midges (GenBank accessions JQ683259-JQ683374). The blood meals were first screened with a species-specific cytochrome b primer pair for cow and if negative with a universal cytochrome b primer pair followed by sequencing to identify mammal or avian blood meal hosts...

  11. Association between land cover and Culicoides (Diptera: Ceratopogonidae) breeding sites on four Danish cattle farms

    DEFF Research Database (Denmark)

    Kirkeby, Carsten; Bødker, Rene; Stockmarr, Anders;

    2009-01-01

    Biting midges of the genus Culicoides are vectors of bluetongue virus. Their larval habitats are poorly known in Northern Europe. Three classes of the CORINE land cover index, found within 300 in of four farms in Denmark, were used to stratify sampling sites for a total of 360 soil core samples...... from 30 sampling points. Soil samples were set up in emergence chambers for hatching adult Culicoides. Two species of Culicoides (C punctatus and C pulicaris) emerged from nine of 12 soil samples from a wet, grazed field with manure. Seventy-two other samples from similar land cover on the three other...... farms were negative. Seven sampling points from pastures were incorrectly classified by CORINE. The remaining 23 sampling points were classified correctly. The visually observed land use was not sufficiently detailed to adequately predict Culicoides breeding sites in this study. The CORINE index failed...

  12. Two new biting midges from Pará, Brazil (Diptera: Ceratopogonidae

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    Rosimeire Lopes da Trindade

    2011-02-01

    Full Text Available Two new species of Culicoides Latreille of the subgenus Haematomyidium Goeldi are described and illustrated based on female specimens from Pará, Brazil. The new species are compared with their similar congeners.

  13. Ten species of Culicoides Latreille (Diptera: Ceratopogonidae) newly recorded from Thailand.

    Science.gov (United States)

    Thepparat, Arunrat; Bellis, Glenn; Ketavan, Chitapa; Ruangsittichai, Jiraporn; Sumruayphol, Suchada; Apiwathnasorn, Chamnarn

    2015-01-01

    A survey of biting midges in animal sheds, mangroves and beaches along the Andaman coastal region in southern Thailand between April 2012 and May 2013 collected 10 species of Culicoides which were not previously known from Thailand. These new records are C. arenicola, C. flavipunctatus, C. hui, C. kinari, C. kusaiensis, C. parabubalus, C. quatei, C. spiculae, C. pseudocordiger and C. tamada. An updated checklist of species of Culicoides reported from Thailand is provided. PMID:26624391

  14. Dermatozoonosis by Culicoides bite (Diptera, Ceratopogonidae in Salvador, State of Bahia, Brazil

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    Italo A. Sherlock

    1964-01-01

    Full Text Available Os autores iniciam com êste, uma série de trabalhos sôbre a Dermatozoonose provocada pela picada de Culicoides, em Salvador, Bahia, Brasil. No presente, tratam das espécies de Culicoides encontradas em Salvador, baseados na coleta de 2.947 exemplares, durante os anos de 1959 a 1963. Encontraram as 4 espécies seguintes; C. (O. paraensis (Goeldi, 1905 C. (O. limonensis Ortiz & Leon, 1955 C. (C. insignis Lutz, 1913 C. (C. flavivenula Costa Lima, 1937. Não reencontraram o C. (C. maruim, Lutz, 1913 assinalado por fox (1948 e WIRTH & BLANTON (1956 para Salvador. Dessas espécies o C. (C. paraensis se mostrou a predominante, abrangendo 98% de exemplares coletados. Apresentam a distribuição das espécies por bairros e respectivas quantidades coletadas. Finalizando, dão as principais características morfológicas das espécies, ilustrando essas com desenhos do material por êles estudado.

  15. Prevalence of Culicoides imicola and other species (Diptera: Ceratopogonidae ateight sites in Zimbabwe : to the editor

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    G.N. Musuka

    2001-07-01

    Full Text Available Presents some prevalence data on Culicoides imicola and Culicoides bolitinos in Zimbabwe. Includes data on other common species, as revealed by collections made over 2 years at 8 widely-separated study sites

  16. Culicoides huaynacapaci, a new species from the Department of Cajamarca, Peru (Diptera, Ceratopogonidae

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    Maria L. Felippe-Bauer

    2008-09-01

    Full Text Available A new species of Culicoides of the subgenus Mataemyia Vargas, Culicoides huaynacapaci Felippe-Bauer, is described and illustrated based on female and male specimens collected biting humans in Department of Cajamarca, in Peruvian Amazonia. The new species is compared with its similar congener C. albuquerquei Wirth & Blanton.Uma nova espécie de Culicoides do subgênero Mataemyia Vargas, Culicoides huaynacapaci Felippe-Bauer, é descrita e ilustrada baseada em exemplares fêmeas e machos coletados picando humanos no Estado de Cajamarca, na Amazônia Peruana. A nova espécie é comparada com a espécie afim, C. albuquerquei Wirth & Blanton.

  17. New Culicoides Latreille of the subgenus Mataemyia Vargas from Pará, Brazil (Diptera: Ceratopogonidae

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    Maria Luiza Felippe-Bauer

    2013-02-01

    Full Text Available Two new species of Culicoides Latreille of the Neotropical subgenus Mataemyia Vargas are described and illustrated based on female specimens from Juruti, Pará, Brazil and compared with their similar congeners. This paper also presents a diagnosis of the subgenus Mataemyia and a systematic key for the identification of the 19 species of the subgenus.

  18. A new Culicoides (Diptera: Ceratopogonidae of the Reticulatus species group from Brazilian Amazon Region

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    Maria Luiza Felippe-Bauer

    2010-11-01

    Full Text Available A new species of the Culicoides reticulatus species group, Culicoides kuripako Felippe-Bauer, is described and illustrated based on females and male specimens from the states of Amazonas and Pará, Brazil. The new species is compared with its similar sympatric congener, Culicoides paucienfuscatus Barbosa.

  19. Effect of temperature on replication of epizootic hemorrhagic disease viruses in Culicoides sonorensis (Diptera: Ceratopogonidae)

    Science.gov (United States)

    Replication of many arboviruses, including some orbiviruses, within the vector has been shown to be temperature-dependent. In general, cooler ambient temperatures slow virus replication in arthropod vectors, whereas viruses replicate faster and to higher titers at warmer ambient temperatures. Prev...

  20. Description of Culicoides lisicarruni (Diptera: Ceratopogonidae), a new species from Cundinamarca, Colombia

    OpenAIRE

    Ligia Moncada; María Cristina Carrasquilla; Gustavo Spinelli; Ingrid Lotta; Nubia Matta

    2010-01-01

    A new species of Culicoides of the subgenus Diphaomyia Vargas from high altitudes of the Andes in Colombia is described and photographied. The species is compared with its similar congener Culicoides marinkellei Wirth & Lee. Data on the collecting site and notes on the species daily activity are also provided.

  1. 陕西省病媒蠓虫研究%Research on Ceratopogonidae of Shaanxi Province

    Institute of Scientific and Technical Information of China (English)

    韩雪玲; 史锋庆; 高建宏; 温煜; 胡淑芳; 刘增加; 何静; 罗晓红

    2009-01-01

    目的 研究掌握陕西省双翅目蠓科Ceratopogonidae昆虫.方法 人帐诱法、畜诱法、灯诱法和网捕法.结果 研究获得蠓科3亚科8属30种,即细蠓亚科Subfamily Leptoconopinae,细蠓属Leptoconops Skuse 2种;毛蠓亚科Subfamily Dasyheleinae,毛蠓属Dasyhelea Kieffer 2种;蠓亚科Subfamily Ceratopogoninae,库蠓属Culicoides Latreille 21种,铗蠓属Forcipomyia Meigen 1种、阿蠓属Alluaudomyia Kieffer 1种,柱蠓属Stilobezzia Kieffer 1种,须蠓属Palpomyia Meigen 1种和绒蠓属Mallochohelea Wirth 1种.库蠓属种类最多,是陕西省双翅目蠓科的主要构成者.陕西省蠓虫白天几乎都有活动,但不同蠓种活动时域不同,库蠓主要是在日出前后和日落前后形成一日活动高峰时域,尤其日落前后活动为甚,而细蠓和非吸血蠓主要是在白天活动.结论 库蠓是陕西省双翅目蠓科的主要构成者,在不同地理区域蠓科种类的组成与优势种群不同,其活动的时域不同,为陕西省双翅目蠓科提供了本底资料.

  2. A new Culicoides (Diptera:Ceratopogonidae of the subgenus Diphaomyia from Peru

    Directory of Open Access Journals (Sweden)

    Maria Luiza Felippe-Bauer

    2005-02-01

    Full Text Available A new species of Culicoides of the subgenus Diphaomyia, Culicoides jurbergi Felippe-Bauer, is described and illustrated based on female specimens collected biting man and with light traps in Peruvian Amazonia. The species is compared with its similar congener mirsae Ortiz.

  3. Description of breeding sites of Culicoides species (Diptera: Ceratopogonidae in Turkey

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    Uslu U.

    2007-06-01

    Full Text Available The aim of this study was to describe the breeding sites of Culicoides species in Konya province, Turkey. The samples taken from 11 different habitats in Konya province were examined for Culicoides species. The collected adult Culicoides specimens were reared in plastic buckets in our laboratory. Among 2,798 specimens reared, 18 species were identified. Culicoides circumscriptus Kieffer, 1918 was the most abundant species reared in the samples taken from mud rich in organic matters near the water reservoirs; C. circumscriptus, C. nubeculosus (Meigen, 1830 and C. shaklawensis Khalaf, 1957 in moist soil with organic matter; C. gejgelensis Dzhafarov, 1964 in moist soils, along watering channels and dripping waters; C. festivipennis Kieffer, 1914 and C. circumscriptus along sewage channel; C. festivipennis in reed sites and along garden watering channels; C. circumscriptus in rain pools; C. odiatus Austen, 1921 and C. circumscriptus in mud near the dams; C. gejgelensis and C. kibunensis Tokunaga, 1937 from sites along the stream. No Culicoides was reared in the samples obtained from livestock dung and tree holes. It was observed that C. circumscriptus, C. festivipennis and C. shaklawensis preferred mud rich in organic matters near the water reservoirs. Culicoides imicola Kieffer, 1913, which is the main vector of bluetongue, was not detected in Konya province. The others vectors of bluetongue, C. obsoletus gr. (Meigen, 1818, C. schultzei gr. (Enderlein, 1908 and C. pulicaris (Linnaeus, 1758 were only obtained in little numbers.

  4. Description of Culicoides lisicarruni (Diptera: Ceratopogonidae, a new species from Cundinamarca, Colombia

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    Ligia Moncada

    2010-12-01

    Full Text Available A new species of Culicoides of the subgenus Diphaomyia Vargas from high altitudes of the Andes in Colombia is described and photographied. The species is compared with its similar congener Culicoides marinkellei Wirth & Lee. Data on the collecting site and notes on the species daily activity are also provided.

  5. Modelling the Abundances of Two Major Culicoides (Diptera: Ceratopogonidae Species in the Niayes Area of Senegal.

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    Maryam Diarra

    Full Text Available In Senegal, considerable mortality in the equine population and hence major economic losses were caused by the African horse sickness (AHS epizootic in 2007. Culicoides oxystoma and Culicoides imicola, known or suspected of being vectors of bluetongue and AHS viruses are two predominant species in the vicinity of horses and are present all year-round in Niayes area, Senegal. The aim of this study was to better understand the environmental and climatic drivers of the dynamics of these two species. Culicoides collections were obtained using OVI (Onderstepoort Veterinary Institute light traps at each of the 5 sites for three nights of consecutive collection per month over one year. Cross Correlation Map analysis was performed to determine the time-lags for which environmental variables and abundance data were the most correlated. C. oxystoma and C. imicola count data were highly variable and overdispersed. Despite modelling large Culicoides counts (over 220,000 Culicoides captured in 354 night-traps, using on-site climate measures, overdispersion persisted in Poisson, negative binomial, Poisson regression mixed-effect with random effect at the site of capture models. The only model able to take into account overdispersion was the Poisson regression mixed-effect model with nested random effects at the site and date of capture levels. According to this model, meteorological variables that contribute to explaining the dynamics of C. oxystoma and C. imicola abundances were: mean temperature and relative humidity of the capture day, mean humidity between 21 and 19 days prior a capture event, density of ruminants, percentage cover of water bodies within a 2 km radius and interaction between temperature and humidity for C. oxystoma; mean rainfall and NDVI of the capture day and percentage cover of water bodies for C. imicola. Other variables such as soil moisture, wind speed, degree days, land cover or landscape metrics could be tested to improve the models. Further work should also assess whether other trapping methods such as host-baited traps help reduce overdispersion.

  6. Morphological and molecular identification of species of the Obsoletus group (Diptera: Ceratopogonidae) in Scandinavia

    DEFF Research Database (Denmark)

    Nielsen, Søren Achim; Kristensen, Michael

    2011-01-01

    in a stereomicroscope. Culicoides obsoletus, Culicoides scoticus, Culicoides chiopterus, and Culicoides dewulfi all belonging to subgenus Avaritia are common in collections in northern Europe. C. obsoletus and C. scoticus often make up more than 50% of the total catch of biting midges. Separation of the females...... identification of the Obsoletus group females. In conclusion, the females of all four species of the Obsoletus group can be separated by a quick morphological method under the stereomicroscope....

  7. Dermatozoonosis by Culicoides' bite (Diptera, Ceratopogonidae in Salvador, State of Bahia, Brazil: IV - A clinical study

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    Italo A. Sherlock

    1965-01-01

    Full Text Available A observação de 211 pacientes com reação intensa à picada do Culicoides, que procuraram tratamento na Clínica dermatológica do Hospital das Clínicas da Universidade da Bahia, durante os anos de 1959 e 1962, permitiu o estudo clínico dessa Dermatozoonose, cujos dados são aqui apresentados. A lesão parece ser de natureza alérgica e devido ao aspecto polimorfo pelo qual se apresenta, essa Dermatose pode lembrar o Prorigo, a Escabiose, as Lesões liquenoide; quando a manifestação é mais intensa torna-se uma verdadeira eczematização; quando há infecção secundária, lembra o impetigo folicular. O estudo histológico da lesão revelou ser ela a de uma inflamação crônica, com vascularites e preivascularites dermo-epidérmica, provàvelmente de natureza alérgica. Para que haja a formação da lesão, são necessários: a substância inoculada pelo inseto e o componente alérgico do indivíduo. Não se conhece a natureza da substância inoculada pelo inseto e as seguintes hipóteses são apresentadas para explicá-la: substâncias enzimáticas ou a histamina existentes nas glândulas salivares do Culicoides. Após a picada do Culicoisdes forma-se no local uma pequena área eritematosa que logo após se transforma em pápula; as pápulas podem desaparecer ou transformarem-se em vesículas; estas ao se romperem dilaceram a superfície cutânea, descamam-na ou pode advir uma infecção secundária e transformam-se em pústulas.

  8. Dermatozoonosis by Culicoides' bite (Diptera, Ceratopogonidae in Salvador, State of Bahia, Brazil: III - Epidemiological aspects

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    Italo A. Sherlock

    1965-01-01

    Full Text Available Nesta terceira contribuiçãos os Autores apresentam os aspectos Epídemiológicos da Dermatozoonose pela picada de Culicoides em Salvador. Salientam que embora a densidade de insetos outros de hábitos antropófilos seja elevada na cidade, as seguintes evidências os conduziram a responsabilizar os Culicoides: conincidência do aparecimento de casos de Dermatozoonose após um período de maior densidade de Culicoides; maior número de casos, desde que a densidade de Culicoides aumentou nos últimos anos; proveni~encia de maior número de casos dos bairros onde há maior infestação de Culicoides. A Dermatozoonose é acentuadamente mais freqüente no sexo feminino. Houve maior número de casos entre os negros, talvez devido a maior freqüencia de negros que procuram tratamento no Hospital das Clínicas. Não há predominância acentuada para determinado grupo etário. Num levantamento que fizeram sôbre a incomodidade do Culicoides observaram que 81% de 593 residências visitadas em diferentes bairros, são incomodadas, sendo o inverno a época de maior incômodo. As horas de maior incômodo, coincidem com a ocorrência horária máxima do Culicoides. Observaram que as medidas usadas pela população para combate ao inseto são inadequadas pois, em 56% das residências não se obtém qualquer resultado. Considerando que nesses último cinco anos a densidade de Culicoides aumentou inexplicàvelmente em Salvador, julgam que os seguintes fatôres participara para que êsse fenômeno ocorresse: a extinção do Serviço de Profilaxia da Febre Amarela em 1956, o qual, indiretamente, por meio de sua "polícia de fócos" combatendo o Aedes aegypti, controlava os Culicoides; o crescimento da cidade, aumentando o número de fossas, já que não existe um sistema de esgotos adequado; e a deficiência do Serviço de Limpeza Pública da Cidade, ocasionando o acúmulo de lixo nos quintais, terrenos baldios e mesmo em logradouros públicos. Essas condições permitiram a existência de uma extensa rêde de "focos potenciais" para a proliferação dos Culicoides que agora infestam a cidade.

  9. Atypical mechanoreceptors in larvae of biting midges Forcipomyia nigra (Diptera: Ceratopogonidae).

    Science.gov (United States)

    Urbanek, Aleksandra; Kapusta, Małgorzata

    2016-09-01

    The dorsolateral setae of Forcipomyia nigra have been investigated. They are mechanoreceptors, sensilla trichoidea, innerved by a single neuron. The tubular body is located at the base of the hair shaft. Although the fine structure of the dorsolateral setae is similar to dorsal setae that perform a double function: secretion of a hygroscopic viscous substance through the pores and mechanoreception dorsolateral setae, they do not secrete any fluid. In both types of setae, trichogen cells (hair forming cells) produce the hair shafts and thereafter do not retract from the cavity of the setae. They contain a large polyploid nucleus and expanded bundles of microtubules. In dorsolateral mechanoreceptors, the microtubules form a network around the nucleus of the trichogen cell and are especially numerous in the cytoplasm invading the interior of the seta, which is evidenced by immunofluorescence light microscopy. No tormogen cell, responsible for the production of the setal socket, was found. Our observations indicate that the dorsolateral setae are solely mechanoreceptors but their trichogen cells reveal some glandular activity. PMID:27428285

  10. Molecular identification of bloodmeals from biting midges (Diptera: Ceratopogonidae; Culicoides Latreille) in Denmark

    DEFF Research Database (Denmark)

    Lassen, Sandra Boline; Nielsen, Søren A; Skovgård, Henrik;

    2011-01-01

    engorged biting midges, and hosts were identified in 115 of 125 analysed specimens (90%). Cow, roe deer, horse, mallard and wood pigeon were identified as hosts. The most abundant host species was cow, which constituted 73.9% of the total identified bloodmeals, but the common wood pigeon was found...

  11. Stilobezzia (Acanthohelea curvistyla (Diptera: Ceratopogonidae, new association of sexes and redescription of male

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    Carla G. CAZORLA

    2015-01-01

    Full Text Available El estudio del material tipo, y de ejemplares de Stilobezzia (Acanthohelea curvistyla y de Stilobezzia (A. megatheca, colectados recientemente, demostró una incorrecta asociación de sexos en ambas especies. El alotipo hembra de S. (A. megatheca pertenece a Stilobezzia (A. curvistyla. Se realiza la correcta asociación de sexos de S. (A. curvistyla, se redescribe el macho de esta especie, se expande su diagnosis y se amplía su distribución geográfica.

  12. Note faunistique sur les Culicoides (Diptera, Ceratopogonidae du Gouvernorat de Monastir (Tunisie

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    Chaker E.

    2005-12-01

    Full Text Available À la suite de l’arrivée de la fièvre catarrhale ovine (FCO en Tunisie, les auteurs rapportent les résultats de la première enquête effectuée dans le Gouvernorat de Monastir. Ils signalent la présence de neuf espèces de Culicoides dont trois sont nouvelles pour le pays (C. paolae, C. imicola, C. newsteadi, ce qui porte à 22 le nombre d’espèces actuellement connues.

  13. The range of attraction for light traps catching Culicoides biting midges (Diptera: Ceratopogonidae)

    DEFF Research Database (Denmark)

    Kirkeby, Carsten; Græsbøll, Kaare; Stockmarr, Anders;

    2013-01-01

    Background Culicoides are vectors of e.g. bluetongue virus and Schmallenberg virus in northern Europe. Light trapping is an important tool for detecting the presence and quantifying the abundance of vectors in the field. Until now, few studies have investigated the range of attraction of light tr...... light trap was estimated to be approximately 15.25 meters. The attraction towards light traps is different from the attraction to host animals and thus light trap catches may not represent the vector species and numbers attracted to hosts.......Background Culicoides are vectors of e.g. bluetongue virus and Schmallenberg virus in northern Europe. Light trapping is an important tool for detecting the presence and quantifying the abundance of vectors in the field. Until now, few studies have investigated the range of attraction of light...... traps. Methods Here we test a previously described mathematical model (Model I) and two novel models for the attraction of vectors to light traps (Model II and III). In Model I, Culicoides fly to the nearest trap from within a fixed range of attraction. In Model II Culicoides fly towards areas...

  14. Control of Culicoides Sonorensis (Diptera: Ceratopogonidae)blood feeding on sheep with long lasting repellent pesticides

    Science.gov (United States)

    Culicoides sonorensis is the primary vector of bluetongue and epizootic hemorrhagic disease viruses in North America. Bluetongue disease is considered one of the most economically important arthropod-borne diseases of sheep in North America because it causes significant morbidity and mortality and ...

  15. Papular dermatitis induced in guinea pigs by the biting midge Culicoides sonorensis (Diptera: Ceratopogonidae)

    Science.gov (United States)

    Histological, ultrastructural, and virological examinations were performed on abdominal skin from guinea pigs after a blood meal by colony-bred biting midges, Culicoides sonorensis. Small, superficial, cutaneous, crateriform ulcers with necrosis of superficial dermis developed at feeding sites and ...

  16. Evaluation of naled applied as a thermal fog against Culicoides furens (Diptera: Ceratopogonidae).

    Science.gov (United States)

    Linley, J R; Parsons, R E; Winner, R A

    1987-09-01

    Naled/diesel oil (1:99), applied as a thermal fog, was tested against the biting midge Culicoides furens. The insects were confined in small cages suspended at 4 heights on poles at progressively greater distances from the fog release point. In terms of population survival 24 hr after treatment, a parabolic equation accurately described the regression of percent survival on distance from the release point. If 10% survival is considered as the maximum acceptable, then the equation predicts adequate control up to 19.6 m (64.3 ft) from the fog release point. PMID:3504923

  17. Abundance of biting midge species (Diptera: Ceratopogonidae, Culicoides spp.) on cattle farms in Korea

    Science.gov (United States)

    Oem, Jae-Ku; Chung, Joon-Yee; Kwon, Mee-Soon; Kim, Toh-Kyung; Lee, Tae-Uk

    2013-01-01

    Culicoides biting midges were collected on three cattle farms weekly using light traps overnight from May to October between 2010 and 2011 in the southern part of Korea. The seasonal and geographical abundance of Culicodes spp. were measured. A total of 16,538 biting midges were collected from 2010 to 2011, including seven species of Culicoides, four of which represented 98.42% of the collected specimens. These four species were Culicodes (C.) punctatus (n = 14,413), C. arakawae (n = 1,120), C. oxystoma (n = 427), and C. maculatus (n = 318). C. punctatus was the predominant species (87.15%). PMID:23388441

  18. Identity and diversity of blood meal hosts of biting midges (Diptera: Ceratopogonidae: Culicoides Latreille in Denmark

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    Lassen Sandra B

    2012-07-01

    Full Text Available Abstract Background Host preference studies in haematophagous insects e.g. Culicoides biting midges are pivotal to assess transmission routes of vector-borne diseases and critical for the development of veterinary contingency plans to identify which species should be included due to their risk potential. Species of Culicoides have been found in almost all parts of the world and known to live in a variety of habitats. Several parasites and viruses are transmitted by Culicoides biting midges including Bluetongue virus and Schmallenberg virus. The aim of the present study was to determine the identity and diversity of blood meals taken from vertebrate hosts in wild-caught Culicoides biting midges near livestock farms. Methods Biting midges were collected at weekly intervals for 20 weeks from May to October 2009 using light traps at four collection sites on the island Sealand, Denmark. Blood-fed female biting midges were sorted and head and wings were removed for morphological species identification. The thoraxes and abdomens including the blood meals of the individual females were subsequently subjected to DNA isolation. The molecular marker cytochrome oxidase I (COI barcode was applied to identify the species of the collected biting midges (GenBank accessions JQ683259-JQ683374. The blood meals were first screened with a species-specific cytochrome b primer pair for cow and if negative with a universal cytochrome b primer pair followed by sequencing to identify mammal or avian blood meal hosts. Results Twenty-four species of biting midges were identified from the four study sites. A total of 111,356 Culicoides biting midges were collected, of which 2,164 were blood-fed. Specimens of twenty species were identified with blood in their abdomens. Blood meal sources were successfully identified by DNA sequencing from 242 (76% out of 320 Culicoides specimens. Eight species of mammals and seven species of birds were identified as blood meal hosts. The most common host species was the cow, which constituted 77% of the identified blood meals. The second most numerous host species was the common wood pigeon, which constituted 6% of the identified blood meals. Conclusions Our results suggest that some Culicoides species are opportunistic and readily feed on a variety of mammals and birds, while others seems to be strictly mammalophilic or ornithophilic. Based on their number, dispersal potential and blood feeding behaviour, we conclude that Culicoides biting midges are potential vectors for many pathogens not yet introduced to Denmark.

  19. Identity and diversity of blood meal hosts of biting midges (Dipterea: Ceratopogonidae: Culicoides Latreille) in Denmark

    DEFF Research Database (Denmark)

    Lassen, Sandra; Nielsen, Søren Achim; Kristensen, Michael

    2012-01-01

    . Species of Culicoides have been found in almost all parts of the world and known to live in a variety of habitats. Several parasites and viruses are transmitted by Culicoides biting midges including Bluetongue virus and Schmallenberg virus. The aim of the present study was to determine the identity...

  20. Fungal biological control agents for integrated management of Culicoides spp. (Diptera: Ceratopogonidae of livestock

    Directory of Open Access Journals (Sweden)

    B. W. Narladkar

    2015-02-01

    Full Text Available Aim: Entomopathogenic fungi Metarhizium anisopliae and Beauveria bassiana had wide host range against insects and hence these are being exploited as fungal bio-pesticide on a large scale. Both fungi are proved pesticides against many crop pests and farmers are well acquainted with their use on the field. Thus, research was aimed to explore the potency of these fungal spores against larval and adult Culicoides midges, a pest of livestock. Materials and Methods: In-vitro testing of both fungal biological control agents was undertaken in Petri dishes against field collected Culicoides larvae, while in plastic beakers against field collected blood-engorged female Culicoides midges. In-vivo testing was undertaken by spraying requisite concentration of fungal spores on the drainage channel against larvae and resting sites of adult Culicoides midges in the cattle shed. Lethal concentration 50 (LC50 values and regression equations were drawn by following probit analysis using SPSS statistical computerized program. Results: The results of this study revealed LC50 values of 2692 mg and 3837 mg (108 cfu/g for B. bassiana and M. anisopliae, respectively, against Culicoides spp. larvae. Death of Culicoides larvae due to B. bassiana showed greenish coloration in the middle of the body with head and tail showed intense blackish changes, while infection of M. anisopliae resulted in death of Culicoides larvae with greenish and blackish coloration of body along with total destruction, followed by desquamation of intestinal channel. The death of adult Culicoides midges were caused by both the fungi and after death growth of fungus were very well observed on the dead cadavers proving the efficacy of the fungus. Conclusion: Preliminary trials with both funguses (M. anisopliae, B. bassiana showed encouraging results against larvae and adults of Culicoides spp. Hence, it was ascertained that, these two fungal molecules can form a part of biological control and alternative to chemical control and, therefore, can be inducted in integrated management programs.

  1. Spatial abundance and clustering of Culicoides (Diptera: Ceratopogonidae) on a local scale

    DEFF Research Database (Denmark)

    Kirkeby, Carsten; Bødker, Rene; Stockmarr, Anders;

    2013-01-01

    Background Biting midges, Culicoides, of the Obsoletus group and the Pulicaris group have been involved in recent outbreaks of bluetongue virus and the former was also involved in the Schmallenberg virus outbreak in northern Europe. Methods For the first time, here we investigate the local...... abundance pattern of these two species groups in the field by intensive sampling with a grid of light traps on 16 catch nights. Neighboring trap catches can be spatially dependent on each other, hence we developed a conditional autoregressive (CAR) model framework to test a number of spatial and non......, and cluster locations shifted between catch nights. No significant temporal autocorrelation was detected. CAR models for both species groups identified a significant positive impact of humidity and significant negative impacts of precipitation and wind turbulence. Temperature was also found to be significant...

  2. The ceratopogonidae and a new species from Liaoning province in China(Diptera:Ceratopogonidae)%辽宁省的蠓类及一新种(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    刘国平; 周旭; 武春光

    2007-01-01

    目的 研究辽宁省蠓类的分布.方法 采用帐诱、灯诱和挥网法采集蠓;收集辽宁省蠓类的文献.结果 辽宁省的蠓科已知7属54种,其中吸血蠓2属49种,即:库蠓属48种,包括1新种,东港库蠓(Culicoides donggangensis sp.nov.),蠛蠓属1种;非吸血蠓4属5种,即:贝蠓属1种,毛蠓属1种,铗蠓属2种和须蠓属1种.记述了已知种在辽宁省的分布.结论 对深入开展辽宁省蠓类的区系分布研究提供了依据.新种模式标本保存在沈阳军区疾病预防控制中心.

  3. Scanning electron microscopy of the antennal sensilla in female Culicoides paraensis (Diptera: Ceratopogonidae Microscopia eletrônica de varredura das sensilas antenais em fêmeas de Culicoides paraensis (Diptera: Ceratopogonidae

    Directory of Open Access Journals (Sweden)

    M. L. Felippe-Bauer

    1989-12-01

    Full Text Available We studied by sanning electron microscopy the number, types, structure and distribution of the antennal sensilla of the medical important ceratopogonid Culicoides paraensis (Goeldi. There are about 174 sense organs on the antenmal flagellum which are classified as sensilla chaetica; sharp-tipped and blunt-tipped (type I and II sensilla trichodea; sensilla basiconica; sensilla coeloconica; sensilla ampullacea and styloconic-type sensilla. The role of antennal sensory organs are discussed regarding the host preference of the biting midges.Estudos sobre o número, tipo, estrutura e distribuição das sensilas antenais do ceratopogonídeo de importância médica, Culicoides paraensis (Goeldi, são realizados com microscopia eletrônica de varredura. Encontram-se aproximadamente 174 órgãos sensoriais no flagelo, os quais são classificados em sensila caética; sensila trichoidea, de ápice afilado e de ápice curvo (tipoI e II; sensila basicônica; sensila ampulácea e sensila do tipo estilocônica. É discutido o papel dos órgãos sensoriais da antena na relação Culicoides/hospedeiro.

  4. A new species and new records of Paryphoconus from the Amazon region of Peru (Diptera: Ceratopogonidae Una nueva especie y nuevos registros de Paryphoconus de la región amazónica de Perú (Diptera: Ceratopogonidae

    Directory of Open Access Journals (Sweden)

    Melina Mauad

    2011-06-01

    Full Text Available Paryphoconus peruvianus, a new species from Peruvian Amazonia is described and illustrated from female and male specimens collected at light in the Department of Cuzco. The species is compared with its similar congener P. anomalicornis Kieffer. Furthermore, P. anomalicornis, P. fusciradialis Spinelli y Wirth and P. oliveirai Lane are firstly recorded from Peru.Se describe e ilustra a Paryphoconus peruvianus, una especie nueva de la Amazonia peruana, a partir de hembras y machos capturados a la luz en el Departamento de Cuzco. Esta especie es comparada con su congénere más similar, P. anomalicornis Kieffer. Además, se registran por primera vez para Perú P. anomalicornis, P. fusciradialis Spinelli y Wirth y P. oliveirai Lane.

  5. Two new species and new records of biting midges of the genus Culicoides from northwestern Argentina (Diptera: Ceratopogonidae)

    OpenAIRE

    Gustavo Ricardo Spinelli; Cecilia Veggiani Aybar; María Julia Dantur Juri; Mercedes Lizarralde de Grosso; Pablo Ignacio Marino

    2013-01-01

    The following two new species of Culicoides from the Argentinean Yungas are described, illustrated and placed to subgenus or species group and compared with related congeners: Culicoides calchaqui Spinelli & Veggiani Aybar and Culicoides willinki Spinelli & Veggiani Aybar. Culicoides daedaloides Wirth & Blanton is recorded for the first time for Argentina and Culicoides pseudoheliconiae Felippe-Bauer is firstly mentioned from the northwestern region of the country.

  6. A redescription of Culicoides griseidorsum Kieffer, 1918, with comments on subgeneric position of some European taxa (Diptera: Ceratopogonidae).

    Science.gov (United States)

    Szadziewski, Ryszard; Filatov, Serhii; Dominiak, Patrycja

    2016-01-01

    Culicoides griseidorsum Kieffer, 1918 is formally redescribed, and the male described and illustrated for the first time. The species is placed within Sensiculicoides Shevchenko, 1977 the subgenus restored from the synonymy with Oecacta Poey, 1853. A checklist of European species placed in subgenera Sensiculicoides and Oecacta is provided. PMID:27394830

  7. Two new Culicoides of the paraensis species group (Diptera:Ceratopogonidae from the Amazonian region of Peru

    Directory of Open Access Journals (Sweden)

    Felippe-Bauer Maria Luiza

    2003-01-01

    Full Text Available Two new species of the Culicoides paraensis species group, C. diversus Felippe-Bauer and C. peruvianus Felippe-Bauer, are described and illustrated based on female specimens from Amazonian region of Peru. A systematic key, table with numerical characters of females, and distribution of species of the C. paraensis group are given.

  8. 陕西省病媒蠓虫小志%Study on Small-Sinica of Ceratopogonidae in Shanxi Province

    Institute of Scientific and Technical Information of China (English)

    史锋庆; 韩雪玲; 胡淑芳; 温煜; 高建宏; 刘增加; 何静; 贺春艳

    2009-01-01

    目的 研究陕西省病媒蠓虫志.方法 帐诱、网捕法、畜诱法、灯诱法和文献资料收集.结果 报道了陕西省双翅目蠓科小志,记述了双翅目蠓科8属30种,即细蠓属Leptoconops Skuse 2种,铗蠓属Forcipomyia Meigen 1种,毛蠓属Dasyhelea Kieffer2种,库蠓属Culicoides Latreille 21种,阿蠓属Alluaudomyia Kieffer 1种,柱蠓属Stilobezzia Kieffer 1种,须蠓属Palpomyia Meigen 1种和绒蠓属Mallochohelea Wirth 1种.结论 为陕西省病媒蠓虫研究提供了科学依据.

  9. The Biting Midge Culicoides sonorensis (Diptera: Ceratopogonidae Is Capable of Developing Late Stage Infections of Leishmania enriettii.

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    Veronika Seblova

    Full Text Available Despite their importance in animal and human health, the epidemiology of species of the Leishmania enriettii complex remains poorly understood, including the identity of their biological vectors. Biting midges of the genus Forcipomyia (Lasiohelea have been implicated in the transmission of a member of the L. enriettii complex in Australia, but the far larger and more widespread genus Culicoides has not been investigated for the potential to include vectors to date.Females from colonies of the midges Culicoides nubeculosus Meigen and C. sonorensis Wirth & Jones and the sand fly Lutzomyia longipalpis Lutz & Nevia (Diptera: Psychodidae were experimentally infected with two different species of Leishmania, originating from Australia (Leishmania sp. AM-2004 and Brazil (Leishmania enriettii. In addition, the infectivity of L. enriettii infections generated in guinea pigs and golden hamsters for Lu. longipalpis and C. sonorensis was tested by xenodiagnosis. Development of L. enriettii in Lu. longipalpis was relatively poor compared to other Leishmania species in this permissive vector. Culicoides nubeculosus was not susceptible to infection by parasites from the L. enriettii complex. In contrast, C. sonorensis developed late stage infections with colonization of the thoracic midgut and the stomodeal valve. In hamsters, experimental infection with L. enriettii led only to mild symptoms, while in guinea pigs L. enriettii grew aggressively, producing large, ulcerated, tumour-like lesions. A high proportion of C. sonorensis (up to 80% feeding on the ears and nose of these guinea pigs became infected.We demonstrate that L. enriettii can develop late stage infections in the biting midge Culicoides sonorensis. This midge was found to be susceptible to L. enriettii to a similar degree as Lutzomyia longipalpis, the vector of Leishmania infantum in South America. Our results support the hypothesis that some biting midges could be natural vectors of the L. enriettii complex because of their vector competence, although not Culicoides sonorensis itself, which is not sympatric, and midges should be assessed in the field while searching for vectors of related Leishmania species including L. martiniquensis and "L. siamensis".

  10. Quantifying dispersal of european culicoides (Diptera: Ceratopogonidae vectors between farms using a novel mark-release-recapture technique.

    Directory of Open Access Journals (Sweden)

    Carsten Kirkeby

    Full Text Available Studying the dispersal of small flying insects such as Culicoides constitutes a great challenge due to huge population sizes and lack of a method to efficiently mark and objectively detect many specimens at a time. We here describe a novel mark-release-recapture method for Culicoides in the field using fluorescein isothiocyanate (FITC as marking agent without anaesthesia. Using a plate scanner, this detection technique can be used to analyse thousands of individual Culicoides specimens per day at a reasonable cost. We marked and released an estimated 853 specimens of the Pulicaris group and 607 specimens of the Obsoletus group on a cattle farm in Denmark. An estimated 9,090 (8,918-9,260 Obsoletus group specimens and 14,272 (14,194-14,448 Pulicaris group specimens were captured in the surroundings and subsequently analysed. Two (0.3% Obsoletus group specimens and 28 (4.6% Pulicaris group specimens were recaptured. The two recaptured Obsoletus group specimens were caught at the release point on the night following release. Eight (29% of the recaptured Pulicaris group specimens were caught at a pig farm 1,750 m upwind from the release point. Five of these were recaptured on the night following release and the three other were recaptured on the second night after release. This is the first time that movement of Culicoides vectors between farms in Europe has been directly quantified. The findings suggest an extensive and rapid exchange of disease vectors between farms. Rapid movement of vectors between neighboring farms may explain the the high rate of spatial spread of Schmallenberg and bluetongue virus (BTV in northern Europe.

  11. 陕西省蠓类调查(双翅目)%Study on Ceratopogonidae(Diptera) species of Shaanxi province

    Institute of Scientific and Technical Information of China (English)

    石淑珍; 刘增加; 宫占威; 张继军

    2009-01-01

    目的 调查了解陕西省蠓科昆虫.方法 网捕法、灯光诱捕、畜诱法和人帐诱法.结果 获得蠓科7属29种,即细蠓属Leptoconops Skuse 2种,毛蠓属Dasyhelea Kieffer 2种,库蠓属 Culicoides Latreille 21种,阿蠓属 Alluaudomyia Kieffer 1种,柱蠓属 Stilobezzia Kieffer 1种,须蠓属 Palpomyia Meigen 1种和绒蠓属 Mallochohelea Wirth 1种.结论 初步了解了陕西省蠓种构成.

  12. Five new species of Culicoides Latreille described from Colombia, yielding a new species list and country records (Diptera: Ceratopogonidae

    Directory of Open Access Journals (Sweden)

    Gustavo R Spinelli

    2009-02-01

    Full Text Available The following five new species of Culicoides from Colombia are described, illustrated and placed to subgenus or species group: Culicoides antioquiensis, Culicoides gabrieli, Culicoides inermis, Culicoides micayensis and Culicoides nigrifemur. C. gabrieli is also known from Peru. When possible, their position in previously published keys is indicated and their features discussed in light of the most recent revisions. A list of 180 Culicoides species known (114 or suspected of being in Colombia (66 is given in a Table. Of these, 12 including the new species are recorded from Colombia for the first time.

  13. Population Genetic Structure and Potential Incursion Pathways of the Bluetongue Virus Vector Culicoides brevitarsis (Diptera: Ceratopogonidae) in Australia.

    Science.gov (United States)

    Tay, W T; Kerr, P J; Jermiin, L S

    2016-01-01

    Culicoides brevitarsis is a vector of the bluetongue virus (BTV), which infects sheep and cattle. It is an invasive species in Australia with an assumed Asian/South East Asian origin. Using one mitochondrial marker (i.e., part of the cytochrome oxidase subunit I gene) and six nuclear markers, we inferred population genetic structure and possible incursion pathways for Australian C. brevitarsis. Nine mitochondrial haplotypes, with low nucleotide sequence diversity (0.0-0.7%) among these, were identified in a sample of 70 individuals from seven sites. Both sets of markers revealed a homogeneous population structure, albeit with evidence of isolation by distance and two genetically distinct clusters distributed along a north-to-south cline. No evidence of a cryptic species complex was found. The geographical distribution of the mitochondrial haplotypes is consistent with at least two incursion pathways into Australia since the arrival of suitable livestock hosts. By contrast, 15 mitochondrial haplotypes, with up to four times greater nucleotide sequence diversity (0.0-2.9%) among these, were identified in a sample of 16 individuals of the endemic C. marksi (sampled from a site in South Australia and another in New South Wales). A phylogenetic tree inferred using the mitochondrial marker revealed that the Australian and Japanese samples of C. brevitarsis are as evolutionarily different from one another as some of the other Australian species (e.g., C. marksi, C. henryi, C. pallidothorax) are. The phylogenetic tree placed four of the species endemic to Australia (C. pallidothorax, C. bundyensis, C. marksi, C. henryi) in a clade, with a fifth such species (C. bunrooensis) sharing a common ancestor with that clade and a clade comprising two Japanese species (C. verbosus, C. kibunensis). PMID:26771743

  14. Population Genetic Structure and Potential Incursion Pathways of the Bluetongue Virus Vector Culicoides brevitarsis (Diptera: Ceratopogonidae in Australia.

    Directory of Open Access Journals (Sweden)

    W T Tay

    Full Text Available Culicoides brevitarsis is a vector of the bluetongue virus (BTV, which infects sheep and cattle. It is an invasive species in Australia with an assumed Asian/South East Asian origin. Using one mitochondrial marker (i.e., part of the cytochrome oxidase subunit I gene and six nuclear markers, we inferred population genetic structure and possible incursion pathways for Australian C. brevitarsis. Nine mitochondrial haplotypes, with low nucleotide sequence diversity (0.0-0.7% among these, were identified in a sample of 70 individuals from seven sites. Both sets of markers revealed a homogeneous population structure, albeit with evidence of isolation by distance and two genetically distinct clusters distributed along a north-to-south cline. No evidence of a cryptic species complex was found. The geographical distribution of the mitochondrial haplotypes is consistent with at least two incursion pathways into Australia since the arrival of suitable livestock hosts. By contrast, 15 mitochondrial haplotypes, with up to four times greater nucleotide sequence diversity (0.0-2.9% among these, were identified in a sample of 16 individuals of the endemic C. marksi (sampled from a site in South Australia and another in New South Wales. A phylogenetic tree inferred using the mitochondrial marker revealed that the Australian and Japanese samples of C. brevitarsis are as evolutionarily different from one another as some of the other Australian species (e.g., C. marksi, C. henryi, C. pallidothorax are. The phylogenetic tree placed four of the species endemic to Australia (C. pallidothorax, C. bundyensis, C. marksi, C. henryi in a clade, with a fifth such species (C. bunrooensis sharing a common ancestor with that clade and a clade comprising two Japanese species (C. verbosus, C. kibunensis.

  15. Description of Culicoides pseudoheliconiae sp.n. from Peruvian Amazon and revalidation of Culicoides contubernalis Ortiz & Leon (Diptera: Ceratopogonidae)

    OpenAIRE

    Maria Luiza Felippe-Bauer; Abraham G. Cáceres; Cristiane S Silva; William Valderrama-Bazan; Antero Gonzales-Perez; Janira M. Costa

    2008-01-01

    A new species of the Culicoides hylas species group, Culicoides pseudoheliconiae Felippe-Bauer is described and illustrated based on female specimens from Peruvian Amazon, and Culicoides contubernalis Ortiz & Leon from Ecuador is resurrected and redescribed as a valid species. A systematic key, table with numerical characters of females of species of the Culicoides hylas group are given.

  16. Two new species and new records of biting midges of the genus Culicoides from northwestern Argentina (Diptera: Ceratopogonidae

    Directory of Open Access Journals (Sweden)

    Gustavo Ricardo Spinelli

    2013-08-01

    Full Text Available The following two new species of Culicoides from the Argentinean Yungas are described, illustrated and placed to subgenus or species group and compared with related congeners: Culicoides calchaqui Spinelli & Veggiani Aybar and Culicoides willinki Spinelli & Veggiani Aybar. Culicoides daedaloides Wirth & Blanton is recorded for the first time for Argentina and Culicoides pseudoheliconiae Felippe-Bauer is firstly mentioned from the northwestern region of the country.

  17. Fly proof net shed for livestock: A novel concept of physical barrier for integrated management of Culicoides spp. (Diptera: Ceratopogonidae

    Directory of Open Access Journals (Sweden)

    B. W. Narladkar

    2014-11-01

    Full Text Available Aim: An age old and time tested technique of mosquito net requiring no energy, used by humans since prehistoric period was the inspiration behind this novel technique of fly proof net shed for livestock. With the aim to develop similar type of net shed for animals, which will protect them at night from biting of range of insects from Culicoides midges to mosquitoes, research was undertaken. Materials and Methods: Net shed with pitch roof (gable type was erected for use of livestock. The open inlet area was covered with 40 mesh size wire net. The roof at attic level was fitted with hurricane type of ventilator. Shed was used for animals at night hours only. vane anemometer was used for estimation of temperature and wind related parameters. Thermal humidity index (THI and air changes were calculated as per the standard formulas. Based on these parameters suitability of shed was judged. Results: It was observed that, due to netting of the shed population of Culicoides and other flies and incidences of their bites at night hours were considerably lowered. As a result, animals were found comfortable, and their body movements undertaken for wiping off these flies were significantly reduced from 196.50 to 22.16. All it accrued to increased milk yield to the tune of 18.97% in the net shed buffaloes as against control shed. Studies on suitability and comfort to animals were tested by estimating THI and air changes per hour in the net shed, which also revealed the estimates in comfortable regimen and ventilation, remained not much affected despite of netting. Other parameters studied for testing its more accuracy by taking other species of animals as kids, for them also, shed was found suitable through estimation of various physiological and behavioral parameters. Finally, the efficacy of shed was judged on the basis of cost effectiveness. Highly encouraging results on the above said parameters endorsed the effectiveness of the technique. Conclusion: A net shed with pitch roof (gable type fitted with hurricane type ventilator at its top serves the purpose of a physical barrier to minimize host-pest contact. Observations recorded in the experiment are sound enough to conclude and to recommend the use of net shed for livestock.

  18. 宁夏双翅目蠓科昆虫研究%STUDY ON CERATOPOGONIDAE OF NINGXIA

    Institute of Scientific and Technical Information of China (English)

    刘增加; 石淑珍; 何静; 高琨; 高丽莉

    2009-01-01

    为了研究掌握宁夏双翅目蠓科Ceratopogonidae昆虫,采用人帐诱法、畜诱法、灯诱法和网捕法对其种群组成和数量动态做了调查研究.研究获得宁夏蠓科4亚科6属40种,即细蠓亚科 Subfamily Leptoconopinae,细蠓属 Leptoconops Skuse 17种;毛蠓亚科 Subfamily Dasyheleinae,毛蠓属 Dasyhelea Kieffer 2种;铗蠓亚科 Subfamily Forcipomyiinae,铗蠓属 Forcipomyia Meigen 3种;蠓亚科 Subfamily Ceratopogoninae,库蠓属 Culicoides Latreille 16种,尼蠓属 Nilobezzia (Kieffer 1种和贝蠓属 Bezzia Kieffer 1种.在采获的标本中以细蠓属和库蠓属种类最多.是宁夏双翅目蠓科的主要构成者.银川平吉堡地区细蠓活动的盛季是在5月中旬至7月上旬,6月中旬为一年活动之最高峰;库蠓活动的盛季是在6月中旬至8月下旬,7月中、下旬为一年活动之最高峰;非吸血蠓活动盛季同库蠓,但一年中的活动高峰是在8月上旬.蠓虫几乎全天都有活动,但不同蠓种活动时域不同.库蠓是日出前和日落后1 h活动高峰的主要构成者,而白天蠓虫活动的高峰,确是以细蠓为主.细蠓的婚舞活动以上午为主,自然界细蠓受精率较低(53.85%),而马厩内受精率较高(94.34%).细蠓平均产卵30枚,产卵后平均寿命4.2天;孳生地主要为陆栖型和水陆过渡型,在地表湿润处其幼虫主要活动于2 cm厚范围.

  19. Limited attractant range of the black-light suction trap for the capture of Culicoides biting midges (Dipetera: Ceratopogonidae)

    NARCIS (Netherlands)

    Elbers, A.R.W.; Meiswinkel, R.

    2016-01-01

    The suction light trap (LT) is a standard tool used to capture Culicoides biting midges, when estimating abundances, and mapping species ranges. The exact range of attraction of the LT is in dispute, however, with several studies indicating the range to vary widely, between 4 and 50 m. In this study

  20. Five new species of Culicoides Latreille described from Colombia, yielding a new species list and country records (Diptera: Ceratopogonidae)

    OpenAIRE

    Gustavo R. Spinelli; Erika Santamaría; Olga L Cabrera; Ronderos, María M.; Suárez, Marco F.

    2009-01-01

    The following five new species of Culicoides from Colombia are described, illustrated and placed to subgenus or species group: Culicoides antioquiensis, Culicoides gabrieli, Culicoides inermis, Culicoides micayensis and Culicoides nigrifemur. C. gabrieli is also known from Peru. When possible, their position in previously published keys is indicated and their features discussed in light of the most recent revisions. A list of 180 Culicoides species known (114) or suspected of being in Colombi...

  1. Foraging range of arthropods with veterinary interest: New insights for Afrotropical Culicoides biting midges (Diptera: Ceratopogonidae) using the ring method.

    Science.gov (United States)

    Bakhoum, M T; Fall, M; Seck, M T; Gardès, L; Fall, A G; Diop, M; Mall, I; Balenghien, T; Baldet, T; Gimonneau, G; Garros, C; Bouyer, J

    2016-05-01

    The identification of blood meal source of arthropod vector species contributes to the understanding of host-vector-pathogen interactions. The aim of the current work was to identify blood meal source in Culicoides biting midge species, biological vectors of internationally important arboviruses of livestock and equids, using a new ecological approach. We examined the correlation between blood meal source identified in engorged Culicoides females collected in a suction light trap and the available vertebrate hosts along four rings (200, 500, 1000 and 2000 m) centered at the trap site and described the foraging range of the three main vector species of veterinary interest present in the study area, Culicoides imicola, Culicoides kingi and Culicoides oxystoma. The study was performed in four sites localized in the Niayes region of Senegal (West Africa) where recent outbreaks of African horse sickness occurred. Blood meal source identification was carried out by species-specific multiplex PCRs with genomic DNA extracted from the abdomen of engorged females collected during nine night collections for twenty-six collections. The four most abundant hosts present in the studied area (horse, cattle, goat and sheep) were surveyed in each ring zone. The blood meal source varied according to Culicoides species and host availability in each site. C. oxystoma and C. imicola females mainly fed on horses readily available at 200 m maximum from the trap location whereas females of C. kingi fed mainly on cattle, at variable distances from the traps (200 to 2000 m). C. oxystoma may also feed on other vertebrates. We discuss the results in relation with the transmission of Culicoides-borne arboviruses and the species dispersion capacities. PMID:26826391

  2. Molecular differentiation of the Old World Culicoides imicola species complex (Diptera, Ceratopogonidae), inferred using random amplified polymorphic DNA markers.

    Science.gov (United States)

    Sebastiani, F; Meiswinkel, R; Gomulski, L M; Guglielmino, C R; Mellor, P S; Malacrida, A R; Gasperi, G

    2001-07-01

    Samples of seven of the 10 morphological species of midges of the Culicoides imicola complex were considered. The importance of this species complex is connected to its vectorial capacity for African horse sickness virus (AHSV) and bluetongue virus (BTV). Consequently, the risk of transmission may vary dramatically, depending upon the particular cryptic species present in a given area. The species complex is confined to the Old World and our samples were collected in Southern Africa, Madagascar and the Ivory Coast. Genomic DNA of 350 randomly sampled individual midges from 19 populations was amplified using four 20-mer primers by the random amplified polymorphic DNA (RAPD) technique. One hundred and ninety-six interpretable polymorphic bands were obtained. Species-specific RAPD profiles were defined and for five species diagnostic RAPD fragments were identified. A high degree of polymorphism was detected in the species complex, most of which was observed within populations (from 64 to 76%). Principal coordinate analysis (PCO) and cluster analysis provided an estimate of the degree of variation between and within populations and species. There was substantial concordance between the taxonomies derived from morphological and molecular data. The amount and the different distributions of genetic (RAPD) variation among the taxa can be associated to their life histories, i.e. the abundance and distribution of the larval breeding sites and their seasonality.

  3. Culicoides (Avaritia) gornostaevae Mirzaeva, 1984 (Diptera: Ceratopogonidae) a possible vector species of the Obsoletus group new to the European fauna

    DEFF Research Database (Denmark)

    Kirkeby, Carsten; Dominiak, Patrycja

    2014-01-01

    Culicoides gornostaevae Mirzaeva, 1984, known previously only from Siberia, is a boreal species included into the Obsoletus group of Culicoides sg. Avaritia. Members of the subgenus can act as vectors of various diseases. In Europe they are involved in the transmission of the Schmallenberg virus...... and bluetongue virus. Culicoides gornostaevae Mirzaeva, 1984 is reported for the first time in Europe with new country records from Norway, Poland and Sweden. Culicoides gornostaevae Mirzaeva, 1984 has not been previously mentioned from Europe, even though there has been an extensive monitoring of Culicoides...

  4. Primera cita de Culicoides paradoxalis Ramilo & Delécolle, 2013 (Diptera, Ceratopogonidae en España

    Directory of Open Access Journals (Sweden)

    Sánchez Murillo, J. M.

    2015-12-01

    Full Text Available The ceratopogonid Culicoides paradoxalis Ramilo & Delécolle, 2013 is recorded for the first time in Spain based on reliable morphological evidence according to the previous descriptions of other authors. A total of 438 females (349 nulliparous and 89 parous and a single male were collected with CDC miniature light traps at three different livestock-associated locations in Extremadura Autonomous Community (Spain in 2014. Most specimens were captured between June and August, suggesting a univoltine pattern for this species extended over summer and early autumn. Although the number of collections of C. paradoxalis is low in comparison with the dominant species, the occurrence of this species in monitoring surveillance programs should deserve specific attention in order to estimate the accurate ratio of potential vectors unmistakably. Interesting information about the period of flight and illustrated morphological features are presented for C. paradoxalis in the current paper.Se cita por primera vez en España el ceratopogónido Culicoides paradoxalis Ramilo & Delécolle, 2013, basándose en evidencias morfológicas de acuerdo a las descripciones previas de otros autores. Un total de 438 hembras (349 nulíparas y 89 paras y un macho se recolectaron con minitrampas de luz CDC en tres localidades ganaderas en la Comunidad Autónoma de Extremadura (España en 2014. La mayor parte de los especímenes fueron capturados entre junio y agosto, mostrando un único período de vuelo que se extendió durante todo el verano y principios del otoño. Aunque el número de capturas de C. paradoxalis es reducido en comparación con los Culicoides dominantes, la aparición de esta nueva especie merece especial atención en los programas de vigilancia entomológica con el fin de estimar inequívocamente la proporción exacta de vectores potenciales. Se presenta en este artículo información de interés sobre el período de vuelo así como fotografías de las características morfológicas de C. paradoxalis.

  5. Quantifying Dispersal of European Culicoides (Diptera: Ceratopogonidae) Vectors between Farms Using a Novel Mark-Release-Recapture Technique

    DEFF Research Database (Denmark)

    Kirkeby, Carsten; Bødker, Rene; Stockmarr, Anders;

    2013-01-01

    following release and the three other were recaptured on the second night after release. This is the first time that movement of Culicoides vectors between farms in Europe has been directly quantified. The findings suggest an extensive and rapid exchange of disease vectors between farms. Rapid movement...... specimens of the Obsoletus group on a cattle farm in Denmark. An estimated 9,090 (8,918–9,260) Obsoletus group specimens and 14,272 (14,194–14,448) Pulicaris group specimens were captured in the surroundings and subsequently analysed. Two (0.3%) Obsoletus group specimens and 28 (4.6%) Pulicaris group...... specimens were recaptured. The two recaptured Obsoletus group specimens were caught at the release point on the night following release. Eight (29%) of the recaptured Pulicaris group specimens were caught at a pig farm 1,750 m upwind from the release point. Five of these were recaptured on the night...

  6. Culicoides sonorensis (Diptera: Ceratopogonidae) is not a competent vector of Cache Valley virus (family Bunyaviridae, genus Orthobunyavirus)

    Science.gov (United States)

    We investigated the susceptibility of Culicoides sonorensis to Cache Valley virus (CVV) (family Bunyaviridae, genus Orthobunyavirus) infection and the potential that it could be a vector or site of virus reassortment. CVV is native to the New World and causes disease in livestock. Infected blood mea...

  7. Prevalence, population dynamics and host preferences of Culicoides spp. (Diptera: Ceratopogonidae of livestock in Marathwada region of Maharashtra State

    Directory of Open Access Journals (Sweden)

    B. W. Narladkar

    2014-09-01

    Full Text Available Aim: The present study is a part of a research project on integrated pest management of livestock pests with reference to Culicoides spp. Study of prevalence, population dynamics and host preferences are the important benchmarks essential for chalking out the strategies of integrated pest management of Culicoides, thus the study was aimed. Materials and Methods: Light trap collections of Culicoides midges and other tiny flies from animal shed from seventeen centers representing entire Maharashtra state were conducted. Similarly, year round collections from host sheds were envisaged to work out host preferences and population dynamics of Culicoides spp. locally prevalent. Multiple regression analysis was employed to define the environmental predictors responsible for ups and downs during different seasons occurring in the geographic region of the present study. Results: Study revealed the prevalence of Culicoides spp., Phlebotomus spp. and Simulium spp. Simultaneous study undertaken by the aid of hand net, collections of fly species from Marathwada region of Maharashtra state yielded additionally, Tabanus spp., Pangonia spp., mosquitoes and other cyclorrhaphan flies. Some of the species are vectors of livestock diseases hence map of the distribution of these pest species is for to reckon risk areas. Population dynamics study on Culicoides spp. in Marathwada region indicated that, (a Culicoides population were persistent throughout the year; (b Two peaks of population, one in the monsoon (August-September and another minor peak occurred during post monsoon/beginning of winter (November of the year. Drastic reduction in the population occurred during the month of May, which is the hottest month in the year. Culicoides collections from the sheds of different host species indicated the preferences for feeding in the ascending order of preference as cattle, sheep, buffaloes and then goats. Conclusion: Prevalence of Culicoides schultzei, Culicoides peregrinus and Culicoides actoni was occurred in the Marathwada region of Maharashtra along with other haematophagus flies. Seasonal population dynamic studies depicted two peaks in the Culicoides population, and peak population observed during the monsoon season. Study on the parameters is essential for the preparation of prediction models and forecasting.

  8. PCR identification of culicoid biting midges (Diptera, Ceratopogonidae of the Obsoletus complex including putative vectors of bluetongue and Schmallenberg viruses

    Directory of Open Access Journals (Sweden)

    Lehmann Kathrin

    2012-09-01

    Full Text Available Abstract Background Biting midges of the Obsoletus species complex of the ceratopogonid genus Culicoides were assumed to be the major vectors of bluetongue virus (BTV in northern and central Europe during the 2006 outbreak of bluetongue disease (BT. Most recently, field specimens of the same group of species have also been shown to be infected with the newly emerged Schmallenberg virus (SBV in Europe. A reliable identification of the cryptic species of this group is fundamental for both understanding the epidemiology of the diseases and for targeted vector control. In the absence of classical morphological characters unambiguously identifying the species, DNA sequence-based tests have been established for the distinction of selected species in some parts of Europe. Since specificity and sensitivity of these tests have been shown to be in need of improvement, an alternative PCR assay targeting the mitochondrial cytochrome oxidase subunit I (COI gene was developed for the identification of the three Obsoletus complex species endemic to Germany (C. obsoletus, C. scoticus, C. chiopterus plus the isomorphic species C. dewulfi. Methods Biting midges of the genus Culicoides caught by UV light traps all over Germany were morphologically pre-identified to species or complex level. The COI region was amplified from their extracted DNA and sequenced. Final species assignment was done by sequence comparison to GenBank entries and to morphologically identified males. Species-specific consensus sequences were aligned and polymorphisms were utilized to design species-specific primers to PCR-identify specimens when combined with a universal primer. Results The newly developed multiplex PCR assay was successfully tested on genetically defined Obsoletus complex material as well as on morphologically pre-identified field material. The intended major advantage of the assay as compared to other PCR approaches, namely the production of only one single characteristic band for each species, could be realized with high specificity and sensitivity. Conclusion To elucidate the biological characteristics of potential vectors of disease agents, such as ecology, behaviour and vector competence, and the role of these haematophagous arthropods in the epidemiology of the diseases, simple, cost-effective and, most importantly, reliable identification techniques are necessary. The PCR assay presented will help to identify culicoid vector species and therefore add to bluetongue and Schmallenberg disease research including vector control and monitoring.

  9. Simuliid blackflies (Diptera: Simuliidae and ceratopogonid midges (Diptera: Ceratopogonidae as vectors of Mansonella ozzardi (Nematoda: Onchocercidae in northern Argentina

    Directory of Open Access Journals (Sweden)

    AJ Shelley

    2001-05-01

    Full Text Available Mansonella ozzardi, a relatively non pathogenic filarial parasite of man in Latin America, is transmitted by either ceratopogonid midges or simuliid blackflies. In the only known focus of the disease in north-western Argentina the vectors have never been incriminated. This study investigated the potential vectors of M. ozzardi in this area. The only anthropophilic species of these Diptera families biting man at the time of the investigation were Simulium exiguum, S. dinellii, Culicoides lahillei and C. paraensis. Using experimentally infected flies S. exiguum and both species of Culicoides allowed full development of microfilariae to the infective stage, with C. lahillei being a more competent host than S. exiguum. Based on these data, biting rates and natural infectivity rates it is probable that at the begininning of the wet season C. lahillei is the main vector of M. ozzardi and both C. paraensis and S. exiguum secondary vectors. Additionally, it was found that a single dose of ivermectin was ineffectual in eradicating M. ozzardi from infected individuals in this area.

  10. New records of predaceous midges from the Middle East, with the description of two new species (Diptera: Ceratopogonidae).

    Science.gov (United States)

    Dominiak, Patrycja; Alwin, Alicja; Giłka, Wojciech

    2014-01-01

    Two new distinctive species of predaceous biting midges of the tribe Ceratopogonini are described and illustrated from the Middle East. Brachypogon freidbergi sp. nov., with a unique Y-shaped gonostylus, is recorded from Israel. We also provide the first records of Brachypogon vitiosus (Winnertz) and B. aethiopicus (Clastrier, Rioux & Descous) from this country, and a key to the adult males of the genus Brachypogon Kieffer from the Middle East. Ceratopogon azari sp. nov., described from Lebanon, shows a distinctive structure of the male genital apparatus, and is the southernmost species of that genus in the Western Palaearctic. PMID:24872285

  11. Three new Scandinavian species of Culicoides (Culicoides): Culicoides boyi sp. nov., Culicoides selandicus sp. nov. and Culicoides kalix sp. nov. (Diptera: Ceratopogonidae)

    Science.gov (United States)

    Kristensen, Michael; Pape, Thomas

    2015-01-01

    Abstract Background In the context of a major monitoring program of Culicoides in Denmark and Sweden due to the appearance of bluetongue disease in 2007–2008, a large number of specimens were collected by light traps and sorted morphologically, with COI barcodes generated for selected specimens. New information Three species are described as new to science based on both morphological and molecular data: Culicoides (Culicoides) boyi sp. nov. (Denmark: Jutland), C. (C.) selandicus sp. nov. (Denmark: Zealand) and C. (C.) kalix sp. nov. (Sweden: Norrbotten). All are diagnosed morphologically as well as by molecular barcoding. A key to slide-mounted females of all Scandinavian species of Culicoides (Culicoides) is presented. PMID:26696760

  12. 库蠓属中国四新纪录种(双翅目,蠓科)%FOUR NEW RECORD SPECIES OF THE GENUS CULICOIDES (DIPTERA,CERATOPOGONIDAE) FROM CHINA

    Institute of Scientific and Technical Information of China (English)

    王飞鹏; 虞以新; 欧阳明安

    2013-01-01

    记述了蠓亚科库蠓属中国4新纪录种:三囊亚属Trithecoides Wirth et Hubert,1959的老挝库蠓Culicoides laoensis Howarth和淡黄库蠓C.luteolus Wirth et Hubert;霍蠓亚属Hoffmania Fox,1948的叮鸟库蠓C.parabubalus Wirth et Hubert 和安酋库蠓C.andrewsi Causey.研究标本收藏于军事医学科学院微生物流行病研究所医学昆虫标本馆.

  13. Study on Culicoides ( Trithecoides ) in China (Diptera:Ceratopogonidae)%中国库蠓属三囊亚属的研究(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    刘国平; 郝宝善; 刘金华; 王春梅; 虞以新

    2004-01-01

    目的调查研究中国库蠓属三囊亚属的种类分布和分类.方法用帐诱、灯诱和挥网采集蠓.结果记述了中国库蠓属三囊亚属的鉴别特征,其在国内的分布现已知有33种,报道了主要鉴别特征的测量值,编制了雌虫和雄虫的分种检索表.结论为我国库蠓属三囊亚属的区系分布和分类研究提供了依据.

  14. A New Species of Culicoides (Beltranmyia) from China (Diptera:Ceratopogonidae)%我国库蠓属带纹亚属一新种(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    瞿逢伊; 曹敏; 刘荣兴

    2010-01-01

    目的 调查上海地区吸血蠓的种类.方法 根据上海浦东机场(芦苇湿地)2007-2009年夜间紫外线灯诱获得的蠓类材料,经初步分类后用树胶酚法制作玻片标本,镜检其形态和量度特征,鉴定蠓种.结果 发现库蠓属一新种,命名为上海库蠓Culicoides (Beltranmyia) shanghaiensis sp. nov..该新种鉴别特征为:①翅无斑,基室具0~7根大毛;②雌蠓复眼分离,小眼间具细毛,喙头比 (P/H) 0.7, 头喙比 (H/P) 1.45,触角比(AR)1.18,触角第3~14节具感器(其中第4~10节的感器出现频率分别为:0.7、0.5、0.5、0.7、0.5、0.8和0.7,呈无规律的缺失),具一个发达的受精囊;③雄蠓阳基侧突基部窄连.新种形态酷似Culicoides homochrous Remm,但该种翅基室密布大毛;雌蠓复眼分离、光裸,头喙比为1.03~1.04;雄蠓阳基侧突分离.此外,沟栖库蠓Culicoides charadraeus Arnaud和稀见库蠓Culicoides rarus Das Gupta与本新种亦相似,但前2种雌蠓复眼光裸、密接(或窄离),触角比>1.61,翅基室无大毛,雄蠓尾器有明显不同.结论 描述采自上海的库蠓属带纹亚属一新种,并与近似种的鉴别作了分析讨论.

  15. Description and comparison of the pupae of a further two Culicoides (Avaritia species from the dung of large herbivores in South Africa (Diptera: Ceratopogonidae

    Directory of Open Access Journals (Sweden)

    Hilda Nevill

    2009-09-01

    Full Text Available In 2007 Nevill, Venter, Meiswinkel & Nevill demonstrated that the pupae of five Culicoides species belonging to the Imicola complex of the subgenus Avaritia could readily be differentiated from one another using various morphological characters. Three of the described species, Culicoides bolitinos Meiswinkel 1989; Culicoides loxodontis Meiswinkel 1992 and Culicoides sp. # 107 (= C. kwagga, Meiswinkel, unpublished thesis 1995, were reared from the dung of large herbivores, which included buffaloes, elephants, white and black rhinoceroses and zebras. However, during that study a further two Avaritia species, neither of which belonged to the Imicola complex, were reared from dung and these are the subject of the present study. For the past 20 years the adults of these two new closely related species have been known as Culicoides sp. # 54 pale form (p.f. Meiswinkel and Culicoides sp. # 54 dark form (d.f. Meiswinkel. The taxonomic description and formal naming of the adults of these two species has yet to be done. The present description and comparison of their pupae show that they are two clearly distinct species; that there is no group of morphological characters that can be used to differentiate these two species from the previously described five species of the Imicola complex; and finally that there was no difference between the pupae of C. sp. # 54 d.f. nor C. sp. # 54 p.f. reared from the dung of different host animals.

  16. A new species of Alluaudomyia (Diptera: Ceratopogonidae) from Jiangxi province, China%江西省阿蠓属一新种(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    刘仰青; 柳小青; 虞以新

    2011-01-01

    2009年10月在江西省井冈山市采获170余只蠓科昆虫,包括阿蠓、库蠓、毛蠓、铗蠓和柱蠓5属,其中发现阿蠓属一新种,命名为海婴阿蠓(Alluaudomyia haiyingi Liu,Liu et Yu,sp.nov.).该新种模式标本收藏于军事医学科学院微生物流行病研究所医学昆虫标本馆.%In October 2009, approximately 170 midegs (including specimens of the genera Alluaudomyia, Culicoides, Dasyhelea, Forcipomyia, and Stilobezzia) were collected in Jinggangshan, Jiangxi province. Among them, a new species of Alluaudomyia was found and named A. Haiyingi. The specimens of the new species were deposited in the Medical Entomology Collection Gallery, Academy of Military Medical Sciences, Beijing, China (20 Dongda Jie, Fengtai, Beijing 100071). Alluaudomyia haiyingi Liu, Liu et Yu, sp. Nov. (Fig. 1)Male, eyes bare, palpus segments 1-5 in propotion of 4:10:12:9:15, with sensory pit on segment 3.Wing length 1.12 mm width 0.37 mm, CR 0.509.The wings of A. Haiyingi Liu, Liu et Yu, sp.nov are similar to A. Marginalis Wirth and Delfinado, 1964, but it clearly differs from A. Marginalis Wirth and Delfinado, 1964 by its hypopygium (Fig. 1-5). The hypopygium of this new species is similar to A streptomera Remm, 1980, but the wings are obviously different between them.Holotype (♂). 2009-10-28, collected from Jinggangshan, Jiangxi province, China. Deposited in the Medical Entomology Collection Gallery, Academy of Military Medical Sciences, Beijing, China (20 Dongda Jie, Fengtai, Beijing 100071).

  17. Integrative taxonomy to investigate species boundaries within Culicoides (Diptera: Ceratopogonidae): a case study using subgenus Avaritia from Australasia and Eastern Asia.

    Science.gov (United States)

    Gopurenko, David; Bellis, Glenn Adam; Yanase, Tohru; Wardhana, April Hari; Thepparat, Arunrat; Wang, Jinglin; Cai, Ducheng; Mitchell, Andrew

    2015-01-01

    In this study, species boundaries were examined for 15 described and 2 undescribed species within the economically important Culicoides subg. Avaritia Fox from Australasia and Eastern Asia. We used an integrative taxonomic approach incorporating DNA barcoding, nuclear gene sequencing, and retrospective morphological analyses. Some arbovirus vector species such as Culicoides fulvus Sen and Das Gupta and Culicoides wadai Kitaoka were genetically and morphologically uniform across sampled distributions, but others including Culicoides actoni Smith and Culicoides brevipalpis Delfinado contained 2 or more genetically independent populations of 'cryptic species' that in some cases were sympatric. Some of these 'cryptic species' exhibited consistent morphological differences, while differences are yet to be found for others species. Additionally, an undescribed species, C. Avaritia sp. No. 3, was found to be synonymous with C. fulvus. These results refine our understanding of the distribution of individual species of C. subg. Avaritia and demonstrate that species descriptions and distribution records need revision for part of the Culicoides fauna. Furthermore, because vector competence studies for most of these species are based entirely on Australian populations, the competence of the putative cryptic species identified elsewhere will require independent assessment. Finally, integrative taxonomic assessment requires genetic and morphological assessment of material from the type localities in order to clarify the status and distribution of species, especially for clades containing cryptic species. International collaboration is needed to facilitate this research. PMID:26741249

  18. 宁夏双翅目蠓科区系分布%Study on the Fauna of Ceratopogonidae in Ningxia Hui Autonomous Region

    Institute of Scientific and Technical Information of China (English)

    贾蕾; 田原; 周林; 施耀勇; 刘增加

    2010-01-01

    目的 研究宁夏蠓科区系分布.方法 帐诱、灯诱、动物诱和网捕法;收集整理宁夏蠓科研究文献资料.结果 宁夏蠓科昆虫已知6属39种,其中细蠓属Leptoconops Skues 17种,毛蠓属Dasyhelea Kieffer 2种,铗蠓属Forcipomyia Meigen 2种,库蠓属Culicoides Latreille 16种,尼蠓属Nilobezzia Kieffer 1种,贝蠓属Bezzia Kieffer 1种.宁夏已知蠓科39种,属于古北界29种,两界共有10种,无东洋界的种类.结论 为宁夏双翅目蠓科区系分布研究提供了依据.

  19. Is the morphology of Culicoides intersexes parasitized by mermithid nematodes a parasite adaptation? A morphometric approach to Culicoides circumscriptus (Diptera: Ceratopogonidae).

    Science.gov (United States)

    Muñoz-Muñoz, Francesc; Ramoneda, Josep; Pagès, Nonito; Pujol, Nuria; Talavera, Sandra

    2016-03-01

    Mermithidae is a family of endoparasitic nematodes known to cause intersexuality in arthropods. Intersexes of the genus Culicoides parasitized by mermithids have been the object of several studies aiming to describe their particular morphology. Culicoides intersexes are specimens with male genitalia and feminized sexually dimorphic structures, i.e. antennae, mouthparts and wings. To date, these specimens have only been described qualitatively and a quantitative approach supported by statistical analysis is lacking. Here we conduct morphometric analyses of sexually dimorphic structures in a sample of Culicoides circumscriptus that includes 34 intersexes with the aim of describing precisely the intersexual morphology. The morphology of antennae and the mouthparts was studied by multivariate statistical analysis of linear measures, and wing form by implementing geometric morphometrics techniques. While intersex wings proved to have a similar size to male wings, their shape was intermediate between males and females. However, when allometric shape variation was removed, the wing shape of intersexes was almost identical to that of females. The intersex antennae were morphometrically of the female type, especially when size variation was considered. In contrast, the measured mouthparts (the labrum and the third palpal segment) were halfway between males and females, even when body size was considered. Overall, the antennae and the wings showed a higher degree of feminization than the mouthparts. These findings indicate that the degree of feminization depends both on the morphological structure and on body size. Moreover, we propose that the feminization of the wings and antennae has an adaptive meaning for the parasite, which would favor female-like traits in order to access more easily its breeding sites, where the parasite has plenty of new hosts to infect. Female-like antennae would be beneficial to detect these sites, while having female-like wings would favor the host's capacity of dispersal to these sites. PMID:26809123

  20. A NEW SPECIES OF CULICOIDES (DIPTERA:CERATOPOGONIDAE) FROM EMEI MOUNTAIN, CHINA%峨眉山的库蠓及一新种描述

    Institute of Scientific and Technical Information of China (English)

    廖忠友; 王飞鹏; 虞以新

    2015-01-01

    本文描述了采自中国四川省峨眉山的库蠓属一新种:蓝腹库蠓Culicoides cyancus Liao, Wang et Yu sp nov.和3种四川省新纪录:棒须库蠓Culicoides clavipalpes Mukerji,1931,冲绳库蠓Culicoides okinawensis Mukerji,1931,东方库蠓Culicoides orientalis Mukerji,1931的描述。所有标本均保存于军事医学科学院微生物流行病研究所医学昆虫标本馆。%In the present paper, eleven species ( including a new species) of the Culicoides Latreille are recognized from Emei Mountain, as listed below:Culicoides arakawaeArakawa, 1910 Culicoides clavipalpis Mukerji, 1931 Culicoides cyancus Liao, Wang et Yu sp nov. Culicoides homotomus Kieffer, 1922 Culicoides huffi Causey, 1938 Culicoides nipponensis Tokonaga, 1955 Culicides obsoletus ( Meigen) , 1818 Culicoides okinawensis Arnaud, 1956 Culicoides orientalis Macfie, 1932 Culicoides oxystoma Kieffer, 1910 Culicoides pictimargo Tokunaga et Shogaki, 1953 Culicoides cyancus Liao, Wang et Yu sp nov. Female Wing length 1.7 mm, wing with 12 pale spots.Eyes bare, third segment of maxillary palpus with a large sensory pit.Antennal AR 1.47.Mandible with 22 teeth.Abdomen blue, with a oval spermatheca. Holotype female, Emei Mountain (29.5N, 103.3E) March 7, 2014, collected with light trap. All specimens are deposited in the Medical Entomology Collection Gallery (20 Dongda Jie, Fengtai, Beijing 100071, China) .

  1. TWO NEW SPECIES OF CULICOIDES (DIPTERA, CERATOPOGONIDAE ) FROM CHINA%库蠓属二新种记述(双翅目,蠓科)

    Institute of Scientific and Technical Information of China (English)

    刘增加; 宫占威; 张继军; 石淑珍

    2003-01-01

    报道了我国西北地区库蠓属Culicoides 2新种,多色库蠓Culicoides multifarious Liu,Gong et Zhang,sp.nov.和崎岖库蠓Cuficoides salebrosus Liu ET Shi,sp.nov..正模标本保存在北京医学昆虫标本馆,副模标本保存在兰州军区军事医学研究所.

  2. Two new species of bloodsucking Culicoides in Fujian Province ( Diptera, Ceratopogonidae )%福建省吸血库蠓二新种(双翅目蠓科)

    Institute of Scientific and Technical Information of China (English)

    陈亢川

    2011-01-01

    目的 报道可能成为人兽共患病传媒的2个吸血库蠓新种--莆田库蠓和渠桥库蠓.方法 对1978年乙脑流调中采集到的吸血蠓样本定种鉴定.结果 莆田库蠓触角第3、11~15节具感觉器,触须感觉器分散在节端半部内侧,无感觉窝形成.翅有明显的淡暗斑,第3缘淡斑明显宽阔,呈纵条形,上缘接触翅前缘,下缘邻接m1脉,腹部有2个发达的受精囊,梨形,略不等大.渠桥库蠓触角第3、5、7、9、11~15节端部感觉器成丛,但无形成感觉器窝.翅有明显淡暗色斑,翅前缘有3淡色斑,第3缘淡斑为一独立的小圆形斑,位于R5室内侧,靠近m1脉,远离翅缘与翅端,长毛仅分布在翅端1/3处,腹部有2个发达的受精囊,梨形,略不等大,有较长的颈.结论 中科院动物研究所确认为新种.

  3. 中国华库蠓亚属及一新种(双翅目:蠓科)%Culicoides (Sinocoides) and A New Species from China (Diptera: Ceratopogonidae)

    Institute of Scientific and Technical Information of China (English)

    刘国平; 周旭

    2006-01-01

    目的 研究中国华库蠓亚属的分布和分类.方法 采用帐诱、灯诱和挥网采集蠓.结果 中国华库蠓亚属已知6种,包括1新种,孔庙库蠓(Culicoides kongmiaoensis sp.nov.).记述了该亚属的鉴别特征及主要鉴别特征的测量值,已知种在国内的分布及分种检索表.结论 为我国华库蠓亚属的分布和分类提供了依据.新种模式标本保存于沈阳军区疾病预防控制中心.

  4. PRELIMINARY REPORT OF BLOOD-SUCKING MIDGES IN HONG KONG (DIPTERA:CERATOPOGONIDAE)%香港地区吸血蠓类初记(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    陈家龙; 陈汝达; 梁志华; 何臻敏; 虞以新

    2007-01-01

    2006年5~6月,在香港大帽山到大屿山的18个不同生境地点进行了蠓科昆虫的调查采集,共采获库蠓、蠛蠓和细蠓3吸血蠓属24种.本文报道了全国新纪录库蠓4种,并描述了潮湿蠛蠓Lasiohelea hygroecia Yu,Liang et Chen sp.nov.和扭曲蠛蠓Lasiohelea thyesta Yu,Chen et He sp.nov.二新种.调查结果显示台湾蠛蠓是香港地区白天多见的吸血缘种,而珠海库蠓Culicoides zhuhaiensis Yu et Hao是从市区绿化地带到沿海林缘都有活动的吸血蠓种.

  5. Response of Culicoides sonorensis (Diptera: Ceratopogonidae) to 1-octen-3-ol and three plant-derived repellent formulations in the field.

    Science.gov (United States)

    Braverman, Y; Wegis, M C; Mullens, B A

    2000-06-01

    The potential attractant 1-octen-3-ol and 3 potential repellents were assayed for activity for Culicoides sonorensis, the primary vector of bluetongue virus in North America. Collections using octenol were low, but numbers in suction traps were greater in the high-octenol treatment (11.5 mg/h) than in the low-octenol treatment (1.2 mg/h) or unbaited control for both sexes. Collections using high octenol, CO2 (approximately 1,000 ml/min), or both showed octenol alone to be significantly less attractive than either of the CO2 treatments and that octenol did not act synergistically with this level of CO2. A plant-derived (Meliaceae) extract with 4.5% of active ingredient (AI) (Ag1000), heptanone solvent, Lice free (2% AI from plant extracts in water), Mosi-guard with 50% Eucalyptus maculata var. citriodora Hook extract, and N,N-diethyl-m-toluamide (deet) were applied to polyester-cotton coarse mesh nets and deployed in conjunction with suction light traps plus CO2. Collections in the trap with deet were 66% lower (P 0.05) less than the untreated (negative) control. Relative to deet, collections in the traps with the lice repellent, Ag1000, and Mosi-guard were reduced by 15, 34, and 39%, respectively (P > 0.05). The method has promise for field screening of potential repellents before on-animal testing. PMID:10901641

  6. Molecular differentiation of Culicoides biting midges (Diptera: Ceratopogonidae) from the subgenus Culicoides Latreille in Denmark

    DEFF Research Database (Denmark)

    Lassen, S. B.; Nielsen, S. Achim; Skovgård, H.;

    2012-01-01

    complexes are hard to distinguish. We evaluated the use of the mitochondrial DNA cytochrome oxidase I gene (COI) barcode region in the identification of species within the subgenus Culicoides. COI barcode sequence divergence within species was <1%, whereas it ranged from 12.5% to 19.8% between subgenus...... impunctatus, and Culicoides grisescens. Additionally, this study confirms the existence of Culicoides halophilus as a valid taxon and presents the first Culicoides deltus barcode sequences. Three additional groups of specimens were identified: Culicoides dk1 with a COI barcode diverging by 14.3% to 17.2% from...... other subgenus Culicoides species and Culicoides Kalix and Culicoides dk3, which diverged by 5.9% from each other and showed 12.5% to 17.6% divergence in COI barcode to subgenus Culicoides specimens....

  7. Delineation of Culicoides species by morphology and barcode exemplified by three new species of the subgenus Culicoides (Diptera: Ceratopogonidae) from Scandinavia

    DEFF Research Database (Denmark)

    Nielsen, Søren Achim; Kristensen, Michael

    2015-01-01

    considered, as well as the evolutionary relationships between species within this genus remains problematic. In recent years molecular barcoding has assisted substantially in the identification of biting midges in the multiple entomological survey projects which were initiated in many European countries...... following the bluetongue outbreak in 2006–2009. These studies revealed potentially new species and “species-complexes” with large genetic and morphological variability. Here we use molecular barcoding, together with morphological analysis, to study subgenus Culicoides Latreille from Scandinavia with focus...... three new species initially separated by DNA barcoding with mitochondrial COI can be distinguished by morphological characters. In this context a key to Scandinavian subgenus Culicoides using wing and maxillary palp characters is presented. The key is including the three new species Culicoides boyi...

  8. Report on Insects of Ceratopogonidae(DIPTERA:CERATOPOGONIDAE)--Results on Siting and First-run Preliminary Survey%海南蠓科昆虫(双翅目:蠓科)本底研究报告(一)——选点和首轮初步调查的结果

    Institute of Scientific and Technical Information of China (English)

    王崇财; 蒙中秋; 谭润谦; 余章汉; 徐选举

    2009-01-01

    [目的]建立海南省虫媒传染病及其传播媒介的监测平台,以便迅速检测媒介生物,防止外来媒介生物的入侵.[方法]选择海口美兰国际机场和三亚凤凰机场附近地带和琼海博鳌一迈汤为重点调查地区,采用挥网和帐诱2种方法,于2009年1月开始调查海南岛蠓科昆虫本底.[结果]采获蠓类590余只,经鉴定为4亚科8属,其中包括库蠓(Culicoides)、蠛蠓(Lasiohelea)和细蠓(Leptoconops)三大吸血蠓属.在各采集地中以凤凰机场采获的蠓类最多,占采获蠓类总数的89.51%;在采获的各种蠓类中,以库蠓所占比例最高,为59.05%.在采集蠓类的过程中同时发现在海南岛蠓、蚊和蚋3科吸血双翅目昆虫在旱冬1月的活动种类.在三亚凤凰机场附近调查发现尖喙库蠓(Culicoides oxystoma Kieffer,1910)、异域库蠓(Culicoides peregrinus Kieffer,1910)以及裸蠓属(Atrichopogon)的蠓类在大气相对湿度低于50%的条件下群舞,其成虫雌雄比例达到1:3和1:6.[结论]本次调查第1次显示海南岛在旱冬季节不同生境内仍然有多属蠓类活动,首次获得的旱冬季节吸血双翅目昆虫同时活动的生态信息;提供了异域库蠓和尖喙库蠓这2种重要吸血蠓虫的生态信息;获得与热带作物播授花粉有关裸蠓在海南岛旱冬季节仍可进行群舞活动繁育种群的新启示.

  9. The salivary secretome of the biting midge, Culicoides sonorensis

    Science.gov (United States)

    Culicoides biting midges (Diptera: Ceratopogonidae) are hematophagous insects with over 1400 species distributed throughout the world. Many of these species are of particular agricultural importance as primary vectors of bluetongue, epizootic hemorrhagic disease and Schmallenberg viruses. Detailed s...

  10. Three new Scandinavian species of Culicoides (Culicoides): “C.boyi sp. nov., C.selandicus sp. nov. and C.kalix sp. nov. (Diptera: Ceratopogonidae)

    DEFF Research Database (Denmark)

    Nielsen, Søren Achim; Kristensen, Michael; Pape, Thomas

    2015-01-01

    BACKGROUND: In the context of a major monitoring program of Culicoides in Denmark and Sweden due to the appearance of bluetongue disease in 2007-2008, a large number of specimens were collected by light traps and sorted morphologically, with COI barcodes generated for selected specimens. NEW INFO...

  11. Toxicity of 5 insecticides to Culicoides oxystoma Kieffer (Diptera:Ceratopogonidae)%5种常用杀虫剂对尖喙库蠓的毒性测定

    Institute of Scientific and Technical Information of China (English)

    陈敏; 王飞鹏; 黄响珠; 黄恩炯

    2016-01-01

    Field-collected Culicoides oxystoma were exposed to different concentrations of commercial insecticides, including chlor-pyrifos, DDVP, deltamethyrin, beta-cypermethrin and beta-cypermethrin��emamectin benzoate via filter paper method, so that the toxicity effects of different insecticides on C.oxystoma were determined. Results showed that toxicities varied significantly among insec-ticides. Biting midges were the most susceptible to deltamethyrin (0.060 mg��L-1), and followed by chlorpyrifos (0.588 mg��L-1), beta-cypermethrin (1.741 mg��L-1), beta-cypermethrin��emamectin benzoate (99.670 mg��L-1), DDVP (600.496 mg��L-1) in a descending order. After exposed to insecticides for 1 h, tested midges were transferred to recovery chamber and observed for anoth-er 24 hours, increased mortality within this period was different under the different concentrations of each insecticide. Relative toxic-ity index was maximal when using deltamethrin as standard reagent.%以野外采集的尖喙库蠓成虫为试验对象,通过滤纸接触法,测定了毒死蜱、敌敌畏、溴氰菊酯、高效氯氰菊酯和高氯��甲维盐等5种常用杀虫剂对尖喙库蠓的毒杀效果.结果表明:5种杀虫剂对蠓的毒性差异显著,毒力大小依次为溴氰菊酯>毒死蜱>高效氯氰菊酯>高氯��甲维盐>敌敌畏,试虫接触药剂1 h的LC50分别为0.060、0.588、1.741、99.670和600.496 mg��L-1;试虫经药剂处理1 h后在恢复筒观察24 h,其在各种药剂不同处理浓度下增加的死亡数不同;以溴氰菊酯为标准药剂,其相对毒力指数最大.

  12. 吉林省吸血蠓的分类研究(双翅目:蠓科)%Classification of blood-sucking midges in Jilin province(Ceratopogonidae:Diptera)

    Institute of Scientific and Technical Information of China (English)

    刘国平; 武春光

    2010-01-01

    目的 研究吉林省吸血蠓的分类.方法 采用帐诱、灯诱和挥网法采集蠓;收集吉林省吸血蠓的文献.结果 记述吉林省的吸血蠓已知2属41种,其中库蠓属(Culicoides)39种;蠛蠓属(Lasiohelea)2种.记述吉林省吸血蠓的主要鉴别特征测量值及库蠓属的雌雄虫分种检索表.结论 为深入开展吉林省吸血蠓的生态学、媒介作用和防治等提供了理论依据.

  13. 安徽淮南南塘风景区蠓种类初步调查(双翅目:蠓科)%Prime research of midge species in Nantang scenic spot of Huainan area in Anhui Province (Diptera: Ceratopogonidae)

    Institute of Scientific and Technical Information of China (English)

    宋福春; 沈静; 田晔

    2013-01-01

    目的 初步调查淮南南塘风景区蠓科昆虫的种群组成情况.方法 采用挥网法采集标本,并制片鉴定标本.结果 采集的48只蠓类标本中,经鉴定隶属于3亚科4属8种,即毛蠓亚科(Subfamily Dasyheleintae)1属,即毛蠓属(Dasyhelea Kieffer)2种;铗蠓亚科(Subfamily Forcipomyiinae)2属,即裸蠓属(Atrichopogon Kieffer)1种和铗蠓属(Forcipomyia Meigen)3种;蠓亚科(Subfamily Ceratopogoninae)1属,即库蠓属(Culicoides Latreille)2种.结论 本研究可为该风景区蠓科昆虫的防治提供科学依据.

  14. 四种吸血蠓类雄虫阳茎中叶的扫描电镜观察(双翅目:蠓科)%Observation of aedeagus of four male biting miges with scanning electron microscope (Diptera:Ceratopogonidae)

    Institute of Scientific and Technical Information of China (English)

    严格; 李豫川; 曹军田

    2005-01-01

    在常见的吸血蠓类中,尖喙库蠓Culicoides oxystoma,台湾蠛蠓Lasiohelea taiwano,低飞蠛蠓Lasiohelea humilauolita和郧县细蠓Leptoconops yunhsienensis四种是我国南方江河流域的常见刺叮骚扰的吸血蠓类,在分类鉴定中维性蠓的尾器是蠓种鉴别的重要依据,为此对这四种吸血蠓的阳茎中叶进行扫描电镜观察,以弥补在普通光学显微镜下观察的不足。

  15. NOTES ON FOREIGN SPECIES AND A NEW SPECIES OF BITING MIDGES FROM QINHUANGDAO PORT, CHINA (DIPTERA: CERATOPOGONIDAE)%秦皇岛港采获外国蠓种及一新种的记述(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    聂维忠; 李俊成; 李德昕; 虞以新

    2003-01-01

    本文报道在渤海湾秦皇岛港锚地从来自美国、澳大利亚以及东南亚地区的船舶上检获外国蠓种,其中有狂怒库蠓Culicoides furens (Poey)、马克库蠓Culicoides marksi Lee et Reye,细齿蠛蠓Lasiohelea tenuidentis Yu et Wirth和丰硕细蠓Leptoconops grandis Carter等国外蠓种,以及海神蠛蠓新种Lasiohelea cymodocea Yu,Li et Nie sp.nov.,这是我国在入境国际航船上发现蠓类的首次报道.由此提示,要重视外国蠓种的入侵.

  16. 中国新疆库蠓属一新种描述(双翅目:蠓科)%DESCRIPTION OF A NEW SPECIES OF THE GENUS CULICOIDES (DIPTERA: CERATOPOGONIDAE) FROM XINGJIANG UYGUR AUT. REG. CHINA

    Institute of Scientific and Technical Information of China (English)

    刘国平; 马德新

    2001-01-01

    中国的库蠓属已知有11个亚属281种.在新疆发现的库蠓属l新种:塔合曼库蠓Culicoides(Culi coides)tahemanensis sp.nov.,正模♀,副模3♂,1♀,1988-07-20,新疆塔什库尔干县塔合曼.本新种与渐灰库蠓Culicoides grisescens Edwards相近似,但后者雌虫翅面径5室和臀室淡斑的形状,雄虫尾器阳茎中叶端部的形状与本新种明显不相同.本新种雄虫尾器第9背板的后缘与日本库蠓C.nipponensis相近似,但后者雌虫翅面径5室、中2室和臀室淡斑的形状,雄虫尾器阳茎中叶的形状与本新种有明显区别.模式标本保存在沈阳军区军事医学研究所,沈阳 110034.%A new species of Culicoides, C. tahemanensis sp. nov. is described from Xingjiang Uygur Aut. Reg.in China. The new species is closely allied to Culicoides grisescens Edwards, but both of them are distinctly different in pale spots of cell R5 and cell A of wring of female, and shape of distal portion of aedeagus of male .The ninth tergum of new species is somewhat allied to C. nipponensis Tokunaga, but distinctly different in pale spots of cell R5, cell M2 and cell A of wing, and shape of aedeagus of male. The type speciemens are deposited in the Institue of Military Medical Sciences, Shenyang Military District, Shengyang 110034, China.

  17. Two New Species of Culicoides from Mt.Bawangling, Hainan, China ( Diptera: Ceratopogonidae )%海南霸王岭发现库蠓属二新种(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    王崇财; 陈星恒; 谭润谦; 虞以新

    2012-01-01

    2009年在海南霸王岭(19.0°N,109.0°E)的昆虫调查采集中,采获许多蠓类,经分类研究,发现其中有二种库蠓新种,分别命名为霸王岭库蠓Culicoides bawanglingensis Yu,Wang and Chen,sp.nov.和曲囊库蠓Culicoides reduncutheca Yu,Wang and Tan,sp.nov..这2种库蠓属新种的模式标本珍藏于医学昆虫标本馆(北京丰台东大街20号,100071).

  18. 中国单囊库蠓亚属的种类分布及分类研究(双翅目:蠓科)%Species Distribution and Taxonomic Study on Subgenus Monoculicoides of Genus Culicoides in China (Diptera:Ceratopogonidae)

    Institute of Scientific and Technical Information of China (English)

    王春梅; 刘国平

    2002-01-01

    目的:调查研究中国单囊库蠓亚属种类分布和分类.方法:采用帐诱、灯诱和挥网法采集蠓.结果:我国单囊库蠓亚属Culicoides (Monoculicoides)已知10种,其中翅痣库蠓C.(M.)stigma为中国新记录,记述了该亚属的种类分布,亚属鉴别特征,主要特征测量值和雌、雄虫分种检索表.结论:为我国单囊库蠓亚属的区系分布和分类研究提供了依据.

  19. 吉林省库蠓属傲蠓亚属二新种(双翅目:蠓科)%Two new species of Culicoides (Fastus) from Jilin province of China (Diptera:Ceratopogonidae)

    Institute of Scientific and Technical Information of China (English)

    刘国平; 任清明; 王峰

    2010-01-01

    报道了采自吉林省库蠓属傲蠓亚属二新种:哈尔巴岭库蠓[Culicoides(Fastus)haerbalingensis LAu et Wang,sp.nov.],该新种与Culicoides pallidicornis Kieffer,1919相近似,但Culicoides pallidicornis触须第3节有大而浅的感觉器窝,臀室有1个淡斑与本新种明显不同;类长白库蠓[Culicoides(Fastas)subchangbaiensis Liu et Ren,sp.nov.]与长白库蠓[Culicoides(Fastus)changbaiensis Qu et Ye,1995)相近似,但Culicoides(Fastus)changbaiensis口甲有棘刺约90个,PR 3.45,2个受精囊等大,与类长白库蠓明显不同.新种模式标本保存于沈阳军区疾病预防控制中心.

  20. A new species of Culicoides (Oecacta) (Diptera: Ceratopogonidae) in China%中国库蠓属屋室亚属新种及新纪录(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    赵宇; 刘国平

    2012-01-01

    A new species of Culicoides (Oecacta) was detected in the identification of the specimens of bloodsucking midges from Shandong province, C. (0.) shandongensis sp. nov. The new species was somewhat allied to C. (0.) zhuhaiensis Yu et Hao, 1988, but distinctly different in the shape of the third segment of the palpus, the pale sport of the distal portion of cell R2, and the female spermathecae. C. (0.) turanicus Gutsevich et Smatov, 1971 was recorded for the first time in China. The type specimen is deposited in the Center for Disease Control and Prevention of Shenyang PLA Command, Shenyang 110034, China.%在鉴定采自我国山东省和西藏自治区的吸血蠓标本中发现1个新种和1个中国新纪录,山东库蠓,新种Culicoides(Oecacta)shandongensis sp.nov..该新种与珠海库蠓[Culicoides (Oecacta) zhuhaiensis Yu et Hao,1988]相近似,但后者触须第3节、翅径端淡斑和受精囊的形状与本新种明显不同.都兰库蠓[Culicoides(Oecacta)turanicus Gutsevich et Smatov,1971]中国新纪录.模式标本保存于沈阳军区疾病预防控制中心(沈阳110034).

  1. Detection of bluetongue virus RNA in field-collected Culicoides spp. (Diptera: Ceratopogonidae) following the discovery of bluetongue virus serotype 1 in white-tailed deer and cattle in Louisiana

    Science.gov (United States)

    In November 2004, bluetongue virus (family Reoviridae, genus Orbivirus, BTV) serotype 1 (BTV-1) was detected for the first time in the United States from a hunter-killed deer in St. Mary Parish, LA. In 2005, sera surveys were conducted on three cattle farms near the area where the deer was found, an...

  2. A NEW SPECIES OF CULICOIDES (FASTUS) (DIPTERA:CERATOPOGONIDAE) FROM CHINA%中国库蠓属傲蠓亚属一新种(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    刘国平; 郭文泽

    2009-01-01

    A new species of Culicoides (Fastus),C.(F.) taipingshanensis sp.nov.from Qingdao Shandong Province,China is described.The new species is closely allied to Culicoides okinawensis Arnaud 1956,but is distinctly different in the sensilla coeloconica of antenna situated on segments 3-14,the third segment of palpus with sensory pit,the lacinia with 27 teeth.The type specimens are deposited in the Centre for Disease Control and Prevention of Shenyang Command,Shenyang 110034,China.%报道了采自山东省青岛库蠓属傲蠓亚属一新种:太平山库蠓Culicoides (Fastus) taipingshanensis sp.nov.本新种与冲绳库蠓 Culicoides okinawensis Amaud 1956相近似,但后者触角嗅觉器分布在3~14节,触须有感觉器窝,小颚有齿27枚与本新种明显不同.模式标本保存于沈阳军区疾病预防控制中心,沈阳110034.

  3. Study on classification and geographic distribution of Culicoides in Xinjiang (Diptera: Ceratopogonidae)%新疆库蠓属种类及地理分布研究(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    马合木提; 郜振国; 张建; 邓红; 李新兰

    2016-01-01

    目的调查新疆库蠓的地理分布.方法采用网捕法、灯诱法采集蠓;参考新疆库蠓地理分布的相关文献.结果2014年7~9月间在新疆22县市采获大量蠓类(biting midges),经鉴定有15种库蠓(Culicoides),其中有朝鲜库蠓(Culicoides koreensis Arnaud,1956),大和库蠓(Culicoides japonicas Arnaud,1956),青海库蠓(Culicoides qinghaiensis Fei et Lee,1984)和类三囊库蠓(Culicoides subsejfadinei Liu,Wang et Hao,1999)4种是新疆首次纪录;度定库蠓(Culicoides dudingstoni Kettle et Lawson,1955)、库轮库蠓(Culicoides kurensis Dzhafarov,1960)、长茎库蠓(Culicoides longipennis Khalaf,1957)3种是中国新纪录.新疆的库蠓属经研究整理现知有66种.结论 本项研究为新疆双翅目蠓科的进一步研究与防控提供了科学依据.

  4. Species Distribution and Taxonomic Study on Culicoides fascipennis Section in China (Diptera: Ceratopogonidae)%中国单带库蠓组的分布与分类研究

    Institute of Scientific and Technical Information of China (English)

    武春光; 刘国平

    2005-01-01

    目的研究中国单带库蠓组的分布和分类.方法采用帐诱、灯诱和挥网法采集蠓,收集中国单带库蠓组的文献.结果我国库蠓属屋室亚属单带库蠓组[Culicoides(Oecacta)fascipennis section]已知13种,分别记述了该组的鉴别特征、主要鉴别特征的测量值、已知种在国内的分布和雌雄虫分种检索表.结论为我国库蠓属屋室亚属单带库蠓组的分布和分类提供了依据.

  5. Culicoides (Sinocoides) and two new species from China (Diptera: Ceratopogonidae)%中国库蠓属华蠓亚属及二新种(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    刘国平; 任清明; 王旭

    2011-01-01

    Objective To determine the species distribution and taxonomy of Culicoides (Sinocoides) in China.Methods Bed nets, light traps and insect nets were used to collect the subjects.Results Eight species of Culicoides (Sinocoides) have been recorded in China, including Culicoides (Sinocoides) malipoensis Liu et Ren, sp.nov.and Culicoides (Sinocoides) nanniwanensis Liu et Wang, sp.nov.The distribution of the known species in China and measurements of the main differential characteristics were described to compile a key list of species.Conclusion The investigation clarified the distribution and composition of Culicoides (Sinocoides) in China.Culicoides (Sinocoides) malipoensis Liu et Ren, sp.nov.is alike to Culicoides subpalpifier Wirth et Hubert, 1989.The latter is noticeably distinguishable by contiguous eyes, palpal ratio (PR) of 2.5 and unequal spermathecae of 3.Culicoides (Sinocoides) nanniwanensis Liu et Wang, sp.nov.is alike to Culicoides opertus Liu et Yu, 1990, but the latter is distinctly characterized by the absence of sensory pits on the third palpus, absence of sensilla coeloconica in the 15th antenna segment, and wings with two pale spots.The type specimens are preserved in the Center for Disease Control and Prevention of Shenyang PLA Command, Shenyang 110034, China.%目的 研究中国库蠓属(Genus Culicoides)华蠓亚属(Subgenus Sinocoides)的种类分布和分类.方法 采用人帐诱、诱虫灯诱和网捕法采集蠓.结果 中国库蠓属华蠓亚属现知8种,其中包括2新种:麻栗坡库蠓[Culicoides (Sinocoides)malipoensis Liu et Ren,sp.nov.]和南泥湾库蠓[Culicoides(Sinocoides)nanniwanensis Liu et Wang,sp.nov.].记述了库蠓属华蠓亚属已知种在国内的分布,主要鉴别特征的测量值,编制分种检索表.结论 为中国库蠓属华蠓亚属的分布和分类研究提供了依据.新种模式标本保存在沈阳军区疾病预防控制中心.

  6. A NEW SPECIES OF CULICOIDES (OECACTA) (DIPTERA,CERATOPOGONIDAE) FROM CHINA%中国库蠓属屋室亚属一新种(双翅目,蠓科)

    Institute of Scientific and Technical Information of China (English)

    刘国平; 马德新

    2011-01-01

    A new species of Culicoides ( Oecacta),Culicoides (Oecacta) balikunensis sp.nov.is described from Xinjiang Uygur Autonomous Region in China.The type specimens are deposited in the Center for Disease Control and Prevention of Shenyang PLA Command,Shenyang.%记述了采自新疆的库蠓属屋室亚Culicoides(Oecacta)1 新种,巴里坤库蠓Culicoides(Oecacta)balikmensis sp.nov,新种模式标本保存在沈阳军区疾病预防控制中心.

  7. 黑龙江省库蠓属一新种(双翅目:蠓科)%A new species of the genus Culicoides (Diptera: Ceratopogonidae)from Heilongjiang province, China

    Institute of Scientific and Technical Information of China (English)

    李明; 张波; 刘国平

    2011-01-01

    报道采自黑龙江省黑河市库蠓属1新种,黑河库蠓,新种Culicoides heiheensis sp.nov..该新种与隆林库蠓(Culicoides longlinensis Yu,1982)雄虫相近似,但隆林库蠓中1室、臀室的淡斑数,第9腹板后缘中部宽凹,第9背板后缘无突起,阳基侧突的形状与该新种明显不同.模式标本保存在黑龙江出入境检验检疫局昆虫标本室(哈尔滨150001).%A new species of the genus Culicoides, C. Heiheensis sp. Nov. Was described in Heihe, Heilongjiang, China. The new species was closely allied to male C. Longlinensis (Yu, 1982), but distinctly different in the number of pale spots in its wing cell Ml and cell A. The ninth sternum had wide caudomdian excavation, its ninth tergum had no apical lobes, and the parameres shape was different from male C. Longlinensis (Yu, 1982). The type specimen was deposited in the Entomology Collection Gallery of Heilongjiang Entry-Exit Inspection and Quarantine Bureau, Harbin 150001,China.

  8. Description of a New Species and a New Record of Culicoides (Diptera: Ceratopogonidae) in China%中国库蠓属新种和新纪录的描述(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    刘国平; 曹毓存; 王旭; 陈继寅

    2011-01-01

    A new species of Culicoides, C. jiulongensis Liu et Wang sp. nov. is described from Sichuan Province, China. The new species is closely allied to Culicoides sublatifrontis Smatov et Isimbekov 1971, but is distinctly different from the spermathecae 2 subequal, antennal sensilla coeloconica present on segments 3, 7, (10)11-14, mandible with 16 teeth, and maxilla with 21 teeth, and H/P 1.03 of female C. sublatifrontis Smatov et Isimbekov, 1971. The new species is also similar to Culicoides latifrontis Shakrizjanova, 1962, but is distinctly different from the AR 1.00(0.94-1.10),PR 1.84 (1.33-2.00), mandible with 15-17 teeth of female C. latifrontis shakriajanova, 1962. A new record in China C.abchazicus Dzhafarov 1964 is described from Liaoning, Jiling Province and Inner Mongolia Autonomous Region. All specimens are deposited in the Centre for Disease Control and Prevention of Shenyang Command, Shenyang 110034, China.%报道了采自四川省九龙县库蠓属一新种:九龙库蠓Culicoides jiulongensis Liu et Wang sp.nov.,本新种与Culicoides sublatifrontis Smatov et Isimbekov,1971 相近似,但后者雌虫2个受精囊等大,触角嗅觉器见于第3,7,(10),11~14,大颚齿16枚,小颚齿21枚,头喙比(H/P)1.03与本新种明显不相同;本新种与Culicoides latifrontis Shakrizjanova,1962也相近似,但后者触角比(AR)1.00 (0.94~1.10),触须比(PR)1.84 (1.33~2.00),大颚齿15~17枚与本新种明显不同.新发现了中国新纪录种:切割库蠓Culicoides abchazicus Dzhafarov,1964.模式标本保存在沈阳军区疾病预防控制中心,沈阳 110034.

  9. 中国库蠓属傲蠓亚属及一新种(双翅目:蠓科)%Culicoides (Fastus) including a new species in China (Diptera: Ceratopogonidae)

    Institute of Scientific and Technical Information of China (English)

    周旭; 刘国平

    2015-01-01

    目的 研究中国库蠓属傲蠓亚属的种类分布和分类.方法 采用人帐诱、诱虫灯诱和挥网法采集蠓.结果 中国库蠓属傲蠓亚属现知12种,其中包括1新种:名山库蠓Culicoides(Fastus)mingshanensis sp.nov..记述了中国库蠓属傲蠓亚属已知12种在国内的分布,主要鉴别特征的测量值,编制分种检索表.结论 为中国库蠓属傲蠓亚属的种类分布和分类研究提供了科学依据.名山库蠓的翅班与秀茎库蠓(Culicoides festivipennis Kieffer,1914)相近似,但后者触角嗅觉器分布在3~15节,触须第3节有感觉器窝与本新种明显不同.新种模式标本保存在沈阳军区疾病预防控制中心(沈阳110034).

  10. A NEW SPECIES OF THE GENUS CULICOIDES (JILINOCOIDES)(DIPTERA: CERATOPOGONIDAE) FROM THE GUANGXI ZHUANG AUTON.REG.,CHINA%广西库蠓属吉林亚属一新种(双翅:蠓科)

    Institute of Scientific and Technical Information of China (English)

    刘国平; 郝宝善

    2003-01-01

    A new species of Culicoides (Jilinocoides ),C.(J.) guangxiensis sp. nov. from Guangxi Zhuang Autonomous Region, China is described. The new species is closely allied to Culicoides qianweiensis Yu 1982, but is distinctly different in the presentation of the sensilla coeloconica, sensory pit of the palpus third segment, number of mandible teeth,pale spot on basal portion of wing and r-m pale spot on wing of female. The type specimen is deposited in the Institute of Military Medical Sciences, Shenyang Military District, Shenyang 110034, China.%中国库蠓属吉林亚属已知有2种.在广西发现的库蠓属吉林亚属一新种:Culicoides(Jilinocoides)guanxiensis sp.nov.,正模♀,1974-08-29,广西壮族自治区凭祥市,郝宝善采.本新种与犍为库蠓Culicoides qianweiensisYu 1982相近似,但后者触角第7、9节有嗅觉器,触须第3节无感觉器窝,大颚齿14枚,翅基淡斑和径中淡斑的形状与本新种明显不同.模式标本保存在沈阳军区军事医学研究所,沈阳110034.

  11. 黑龙江省吸血蠓一新种描述(双翅目:蠓科)%A new species of bloodsucking midge from Heilongjiang province of China(Diptera: Ceratopogonidae)

    Institute of Scientific and Technical Information of China (English)

    杨军; 李明; 刘国平

    2011-01-01

    在鉴定采自黑龙江省逊克口岸的吸血蠓标本期间,发现库蠓属一新种,逊克库蠓Culicoides xunkeeasis sp.nov..该新种的翅斑与Culicoides corniculus Liu et Chu,1981和Culicoides koreensis Arnaud,1956相近似,但二者雄虫尾器阳茎中叶和阳基侧突的形状与本新种明显不同.新种模式标本保存于黑龙江出入境检验检疫局标本馆.%A new species of Culicoides was described during the identification specimens of bloodsucking midges from Xunke ports, Heilongjiang province, C.xunkeensis sp.nov.The new species is somewhat allied to C.corniculus Liu et Chu, 1981 and C.koreensis Arnaud, 1956 in the wing spots, but they are distinctly different in the shapes of aedeagus and parameres of male genitalia.The type specimen is deposited in the Entomology Collection Gallery of Heilongjiang Entry-Exit Inspection and Quarantine Bureau of the P.R.China, Haerbin 150001, China.

  12. A new species and new record of midges from Anning, Yunnan (Diptera: Ceratopogonidae)%云南省安宁蠓类一新种及一新纪录(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    余静; 王飞鹏; 石清明; 陈锚锚; 虞以新; 张富强; 范泉水

    2015-01-01

    该文报道了采自云南省安宁疗养院库蠓一新纪录和铗蠓一新种:兴安库蠓(Culicoides sinanoensis Tokunaga)为云南省新纪录,也是我国东洋区的首次记录.该新种的翅面、触须及阳基侧突与Forcipomyia(F.)declivisDebenham,1987相近似,但后者足有环斑,阳茎中叶呈盾形,体无蓝绿色泽与本新种明显不同.本新种与Forcipomyia(F.)adjectaTokunaga,1959也很近似,但其尾器腹板中部无凹陷,阳茎中叶宽可与本新种鉴别.模式标本收藏于医学昆虫标本馆(北京市丰台区东大街20号,100071).

  13. Two new species and a new record of biting midges from Emei Mountain, China (Ceratopogonidae, Diptera)%峨嵋山发现蠓类二新种及一新纪录(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    邓成玉; 廖忠友; 张有植; 莫东平; 虞以新

    2011-01-01

    The present paper deals with two new species and a new record of biting midges from Mt. Emei, Emei County,Sichuan Province, China. All specimens were collected with net trap during Sept. 15-29 , 2010 from Mt. Emei ( 29. 5°N,103. 3°E ), Sichuan Province, and are deposited in the Collection of Medical Entomology of Beijing Institution of Microbiology and Epidemiology ( 20 Dongdajie Ave. , Fengtai Dis. , Beijing 100071 , China ).1 Atrichopogon emeiensis Deng, Liao et Yu, sp. nov. ♂ ( Fig. 1 )Compound eyes bare, wing length 2. 07 mm, width 0. 55 mm. Scutellum with 6 long bristles, hind tibia with 9 terminal bristles.This new species is quite specific in very short antenna segment 15 , differing distinctly from that of other species of the genus. The new species is closely allied to Atrichopogon schizonyx Giles et Wirth, 1982 and Atrichopogon setosilateralis Borkent et Picado, 2004 , but eyes are pubescent in the allied species, distinctly differing from the new species.Type: holotype ♂ .2 Stilobezzia lijiangi Yu, Zhang et Mo, sp. nov. ♂♀ ( Fig. 2 )Compound eyes bare, wing unmarked, surface without macrotrichia; length ♂ 1. 09 mm, ♀ 1. 15 mm;width ♂ 0. 32 mm ♀ 0. 45 mm, Scutellum with 4 long bristles, hind tibia with 5 terminal bristles.The male of new species is somewhat related to Stilobezzia blaesopira Yu et Deng, 2005. and St. unifaciatus Tokunaga, 1963 , especially in the aedeagus, but distinctly different from the related species in the wing surface without macrotrichia and mid leg TR >3. Types: holotype ♂ , paratype ♀ .3 Stilobezzia robusta Das Gupta et Wirth, 1968 ( Fig. 3 ) New record in China. Material exanmined : 1 ♂ .Distribution : Malaysia ; China.%2010年9月中下旬在四川省峨眉县峨眉山(29.5°N,103.3°E)西南麓进行了挥网采集,获得蠓类2亚科10属11种,其中有2个新种:峨眉裸蠓(Atrichopogon emeiensis Deng,Liao et Yu sp.nov.)和李江柱蠓(Stilobezzia lijiangi Yu,Zhang et Mo,sp.nov.);以及1种我国新纪录:柔软柱蠓(Stilobezzia robusta Das Gupta et Wirth).同时发现趋光蠛蠓(La.phototropia Yu et Liu)雄虫和泸定毛蠓(Dasyhelea ludingensis)的雌虫在黄昏时分参与雄性摇蚊的群舞团.本文描述新种模式标本都收藏于军事医学科学院微生物流行病研究所医学昆虫标本馆.

  14. Principal climatic and edaphic determinants of Culicoides biting midge abundance during the 2007–2008 bluetongue epidemic in the Netherlands, based on OVI light trap data

    NARCIS (Netherlands)

    Scolamacchia, F.; van den Broek, J.; Meiswinkel, R.; Heesterbeek, J.A.P.; Elbers, A.R.W.

    2014-01-01

    Palaearctic Culicoides midges (Diptera: Ceratopogonidae) represent a vital link in the northward advance of certain arboviral pathogens of livestock such as that caused by bluetongue virus. The effects of relevant ecological factors on weekly Culicoides vector abundances during the bluetongue virus

  15. The Mondrian Matrix: Culicoides prevalence and seasonal abundance during the 2006-2008 epizootic of bluetongue in the Netherlands

    NARCIS (Netherlands)

    Meiswinkel, R.; Scolamacchia, F.; Dik, M.; Mudde, J.; Dijkstra, E.; Ven, van der I.J.K.; Elbers, A.R.W.

    2014-01-01

    During the northern Europe epidemic of bluetongue (BT), Onderstepoort-type blacklight traps were used to capture Culicoides Latreille (Diptera: Ceratopogonidae) biting midges weekly between November 2006 and December 2008 on 21 livestock farms in the Netherlands. Proven and potential vectors for the

  16. The dying of the light: crepuscular activity in Culicoides and impact on light trap efficacy at temperate latitudes

    NARCIS (Netherlands)

    Meiswinkel, R.; Elbers, A.R.W.

    2016-01-01

    The light trap is the tool of choice for conducting large-scale Culicoides (Diptera: Ceratopogonidae) vector surveillance programmes. Its efficacy is in doubt, however. To assess this, hourly changes in Culicoides activity over the 24-h diel were determined comparatively by way of light trapping and

  17. Management of North American Culicoides biting midges: Current knowledge and research needs

    Science.gov (United States)

    Culicoides biting midges (Diptera: Ceratopogonidae) are biological vectors of two important viruses infecting North American ruminants: bluetongue (BTV) and epizootic hemorrhagic disease (EHDV). While these viruses have been identified for over 60 years, we still lack an adequate understanding of t...

  18. Studying Culicoides vectors of BTV in the post-genomic era: resources, bottlenecks to progress and future directions

    Science.gov (United States)

    Culicoides biting midges (Diptera: Ceratopogonidae) are a major vector group responsible for the biological transmission of a wide variety of globally significant arboviruses, including bluetongue virus (BTV). In this review we examine current biological resources for the study of this genus, with a...

  19. Understanding and exploiting olfaction for the surveillance and control of Culicoides biting midges

    Science.gov (United States)

    Culicoides midges (Diptera: Ceratopogonidae) are found worldwide with the exception of only a few countries including New Zealand, Patagonia, the Hawaiian Isles and Antarctica. They are a nuisance pest to human beings, but transmit a number of diseases that mainly affect livestock. Like many haema...

  20. A New Species of Culicoides and Newly Found Male of Culicoi des pelius Liu et Yu (Diptera: Ceratopogonidae) from Tibet,China%西藏库蠓一新种及黑色库蠓雄虫的发现(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    刘国平; 邓成玉

    2000-01-01

    本文报道了西藏库蠓一新种,聂拉木库蠓C ulicoides nielamensis Liu et Den,sp.nov.;黑色库蠓Culicoides pelius Liu et Yu 1990雄虫的发现.新种模式标本保存在医学昆虫标本馆(北京100071).

  1. Sucking-blood Midges and Description of Newly Found Male of Culicoides yadongensis from Southern Frontiers of Tibet in China (Diptera:Ceratopogonidae)%西藏南部边境地区的吸血蠓及亚东库蠓雄虫描述(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    张有植; 邓成玉; 薛群力; 陈川; 刘国平

    2003-01-01

    目的研究西藏南部边境地区吸血蠓的种类分布.方法人帐诱、诱虫灯诱和挥网法采集蠓.结果在西藏南部边境5县采获吸血蠓3属65种,其中库蠓属62种,蠛蠓属2种,细蠓属1种.首次发现了亚东库蠓的雄虫;首次证实了西藏有蠛蠓的存在.记述了已知吸血蠓种的县区分布.结论为西藏南部边境地区吸血蠓的区系分布研究提供了依据.

  2. 青海毛蠓属一新种记述及三种主要蠓虫日活动节律的初步观察(双翅目:蠓科)%Description of a New Species of the Genus Dasyhelea and Observations on Day Action Rhythm of Three Chief Species of Midges (Diptera: Ceratopogonidae)in Qinghai Province,China

    Institute of Scientific and Technical Information of China (English)

    刘增加; 石淑珍; 宫占威; 张继军

    2005-01-01

    描述了采自青海省共和县江西沟青海湖岸边毛蠓属Dasyhelea 1新种:青海毛蠓Dasyhelea qinghaiensis Liu et Shi,sp.nov.,并初步观察了青海毛蠓 Dasyhelea qinghajensis Liu et Shi,sp.nov.、格尔木细蠓 Leptoconops(Holoconops) germuensis Liu et Yu和原野库蠓Culicoides homotomus Kieffer的一日飞舞活动规律.模式标本保存于中国医学昆虫标本馆和兰州军区军事医学研究所标本室.

  3. 库蠓属一新种及瑞丽库蠓雄虫的描述(双翅目:蠓科)%Description of a species of Culicoides and a male Culicoides ruiliensis Lee newly found from China (Diptera:Ceratopogonidae)

    Institute of Scientific and Technical Information of China (English)

    刘国平; 邓成玉

    2010-01-01

    在鉴定采自我国西藏自治区和云南省的库蠓标本期间,新发现库蠓属一新种和瑞丽库蠓(Culicoides ruliensis Lee,1980)雄虫.易贡库蠓,新种Culicoides yigongensis sp.nov..该新种的翅斑与Culicoides chengduensis Zhuo et Lee,1984和Culicoides liubaensis Liu et Wu 2005相近似,但这二者雄虫阳茎中叶和阳基侧突的形状与本新种明显不同.瑞丽库蠓新发现雄虫,新种模式标本保存于沈阳军区疾病预防控制中心.

  4. Notes on the introduced living midges and a new species of genus Bezzia (Diptera: Ceratopogonidae) collected on entry ships arrived at Qinhuangdao port%秦皇岛港入境船舶截获输入性活蠓及贝蠓属一新种(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    聂维忠; 薄景信; 杨晨光; 朱世营; 虞以新

    2015-01-01

    目的 调查了解入境国际航行船舶携带输入性蠓类的情况.方法 在秦皇岛港检疫锚地停泊的入境船舶生活区内采集输入性活蠓并进行分类鉴定.结果 2014年4-6月在5艘入境船舶上捕获33只输入性活蠓,经分类鉴定隶属于蠓科的蠓科库蠓属Culicoides和贝蠓属Bezzia及其他属部分种类.其中在来自朝鲜大安港(DAE AN)的塞拉利昂籍“龙岗7号”入境船舶上采获一只雄性贝蠓经鉴定为新种,遂以该船舶名称命名为龙岗贝蠓(Bezzia longgangi Yu,Nie et Yang sp.nov.),这是我国第三次报道在入境船舶上捕获输入性贝蠓新种.所携带的部分库蠓分别为环斑库蠓(C.circumscriptus Kieffer,1918)、荒川库蠓(C.arakawae Arakawa,1910)和尖喙库蠓(C.oxystoma Kieffer,1910).结论 此次发现再次提示我们应高度重视国外蠓类等外来生物的入侵.

  5. Three new Scandinavian species of Culicoides (Culicoides):Culicoides boyi sp. nov., Culicoides selandicus sp. nov. and Culicoides kalix sp. nov. (Diptera: Ceratopogonidae)

    OpenAIRE

    Nielsen, Søren Achim; Kristensen, Michael; Pape, Thomas

    2015-01-01

    Background In the context of a major monitoring program of Culicoides in Denmark and Sweden due to the appearance of bluetongue disease in 2007–2008, a large number of specimens were collected by light traps and sorted morphologically, with COI barcodes generated for selected specimens. New information Three species are described as new to science based on both morphological and molecular data: Culicoides (Culicoides) boyi sp. nov. (Denmark: Jutland), C. (C.) selandicus sp. nov. (Denmark: Zea...

  6. 吉林省吸血蠓类名录及一新种描述(双翅目:蠓科)%Catalogue of Blood-sucking Midges of Jilin Province in China with Description of A New Species(Diptera: Ceratopogonidae)

    Institute of Scientific and Technical Information of China (English)

    刘国平; 武春光; 王兴亚

    2006-01-01

    目的 调查吉林省的吸血蠓.方法 采用帐诱、灯诱和挥网法采集蠓;收集吉林省吸血蠓的文献.结果 记述延边库蠓1新种(Culicoides yanbianensis);给出吉林省分布的2属39种吸血蠓名录及其分布.新种模式标本保存在沈阳军区疾病预防控制中心.结论 为深入开展吉林省吸血蠓的研究提供了依据.

  7. A STUDY OF THE MORPHOLOGICAL VARIABILITY AND DESCRIPTION OF THREE SUBSPECIES OF CULICOIDES CIRCUMSCRIPTUS KIEFFER IN DIFFERENT AREAS OF CHINA (DIPTERA:CERATOPOGONIDAE)%我国不同地区环斑库蠓Culicoides circumscriptus Kieffer形态变异的研究及3亚种描述(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    薛健; 刘国平; 虞以新

    2003-01-01

    根据采自我国不同地区的环斑库蠓形态特征与地理分布的比较研究,描述了3个不同地区分布的亚种,即分布于海岛与海岸的环斑库蠓马祖亚种Culicoides circumscriptus matsuensis,分布于我国北纬35°以北地区的环斑库蠓典型亚种C.c.circumscriptius和分布于我国北纬30°以南地区的环斑库蠓南方亚种C.c.meridionalis subsp.nov.,而北纬30°~35°之间为后2亚种的过渡带.

  8. Catalogue of hematophagous midges in Luobei county, China: a new species and a new record of genus Culicoides (Diptera: Ceratopogonidae)%萝北县吸血蠓名录及一新种和一中国新纪录(双翅目:蠓科)

    Institute of Scientific and Technical Information of China (English)

    梁慧杰; 麻惠新; 富英群; 刘国平

    2014-01-01

    目的 调查黑龙江省萝北县吸血蠓种类分布.方法 采用诱虫灯和挥网法采集吸血蠓.结果 2009-2013年在萝北县采获吸血蠓26 827只,计1属30种,其中包括一新种和一中国新纪录:萝北库蠓,新种Culicoides luobeiensis Liang et Liu,sp.nov..该新种与类环斑库蠓(C.subcircumscriputus Yu,1982)相近似,但类环斑库蠓翅径5室和中1脉端部各有1个小淡斑;雌虫触角嗅觉器分别在3、11~14节;雄虫尾器第9腹板膜有微毛,第9背板后缘侧突细长,抱器基节腹踝缺如,阳基侧突基部内侧无小刺突与本新种明显不同.阿拉库蠓[C.alazanicus Dzhafarov,1961(♀)]为中国新纪录.结论 为黑龙江省萝北县吸血蠓的种类分布提供了科学依据.模式标本保存在沈阳军区疾病预防控制中心(沈阳110034).

  9. 黑龙江省萝北口岸库蠓属一新种及—中国新纪录(双翅目:蠓科)%Genus Culicoides (Diptera: Ceratopogonidae) from Luobei port of Heilongjiang province in China: a new species and a new record in China

    Institute of Scientific and Technical Information of China (English)

    梁慧杰; 富英群; 刘国平

    2012-01-01

    报道采自黑龙江省萝北口岸库蠓属一新种及一中国新纪录:丽炜库蠓,新种Culicoides liweiae sp.nov..该新种与戈氏库蠓相近似,但戈氏库蠓翅径2室端部1/2淡色,径端淡斑近三角形,基室无大毛,雄虫两侧抱器基节的腹踝不连接,第9腹板后缘中部凹陷呈“V”形与该新种明显不同.该新种雄虫第9腹板与兴安库蠓也相近似,但二者雄虫抱器基节的腹踝、第9背板和阳茎中叶的形状明显不同.似蕨库蠓(雌虫)(C.filicinus Gornostaeva et Gachegova,1972)为中国新纪录.模式标本保存在黑龙江出入境检验检疫局昆虫标本室(哈尔滨150001).%A new species of the genus Culicoides, C. liweiae sp. nov. from Luobei port, Heilongjiang province, China is reported. The new species is closely allied to male C. gornostaevae Mirzaeva, 1984, but what is distinctly different between them is that the latter is pale in the distal half of cell R2 with a nearly triangle pale spot in the distal portion of cell R wing, no mocrotrichia in the basal cell of wing, the ventra root of two lateral basistly not continuous, and the ninth sternum in "V" caudomedion excavation. The ninth sternum of the male of this new species is also similar to that of C. sinanoensis Tolcunaga, 1937, but they are distinctly different in the shapes of ventra root basistly, ninth tergum, and aedeagus. C. filicinus Gornostaeva el Gachegova, 1972 ( 9) is recorded for the first time in China. The type specimen is deposited in the Entomology Collection Gallection Gallery of Heilongjiang Entry-Exit Inspection and Quarantine Brueau of the P. R. China (Haerbin 150001, China).

  10. Assessment of the repellent effect of citronella and lemon eucalyptus oil against South African Culicoides species

    OpenAIRE

    Gert J. Venter; Karien Labuschagne; Solomon N.B. Boikanyo; Liesl Morey

    2014-01-01

    The use of insect repellents to reduce the attack rate of Culicoides species (Diptera:Ceratopogonidae) should form part of an integrated control programme to combat Africanhorse sickness and other diseases transmitted by these blood-feeding midges. In the presentstudy the repellent effects of a commercially available mosquito repellent, a combinationof citronella and lemon eucalyptus oils, on Culicoides midges was determined. The numberof midges collected with two 220 V Onderstepoort traps f...

  11. A comparison of commercial light-emitting diode baited suction traps for surveillance of Culicoides in northern Europe

    OpenAIRE

    Hope, Andrew; Gubbins, Simon; Sanders, Christopher; Denison, Eric; Barber, James; Stubbins, Francesca; Baylis, Matthew; Carpenter, Simon

    2015-01-01

    Background The response of Culicoides biting midges (Diptera: Ceratopogonidae) to artificial light sources has led to the use of light-suction traps in surveillance programmes. Recent integration of light emitting diodes (LED) in traps improves flexibility in trapping through reduced power requirements and also allows the wavelength of light used for trapping to be customized. This study investigates the responses of Culicoides to LED light-suction traps emitting different wavelengths of ligh...

  12. Freshwater invertebrate fauna of Nuku Hiva island (French Polynesia) : data during a rainy season

    OpenAIRE

    Fossati, Odile; Gibon, François-Marie; Danigo, Anne-Hélène

    1992-01-01

    The streams of Nuku-Hiva Island, Marquesas Archipelago, were sampled during the rainy season in June 1990. The invertebrate fauna was scarce and had a low diversity. Gastropoda and Decapoda accounted for the major part of the biomass. Insects were represented by #Simuliidae$, #Chironomidae$ and a few #Ceratopogonidae$, #Coleoptera$ and #Zygoptera$. #Oligochaeta$ were numerically important. The role of insularity and the effects of hydraulic conditions on this fauna are briefly discussed. (Rés...

  13. Schmallenberg virus in Germany 2011–2014: searching for the vectors

    OpenAIRE

    Kameke, Daniela; Werner, Doreen; Hoffmann, Bernd; Lutz, Walburga; Kampen, Helge

    2015-01-01

    Following the emergence of Schmallenberg virus (SBV) in 2011, 21,397 culicoid biting midges (Diptera: Ceratopogonidae) from targeted and non-targeted sampling activities carried out during the summer months of 2011 to 2013 and in late 2014 in various regions in Germany were analyzed for the virus by real-time RT-PCR. While no SBV was found in biting midges collected during 2011 and 2013, 2 out of 334 pools including 20 and 22 non-engorged females of the Obsoletus complex sampled in 2012 teste...

  14. Insectos acuáticos de la Meseta del Somuncura, Patagonia, Argentina. Inventario preliminar Aquatic insects from Somuncura plateau, Patagonia, Argentina. Preliminary inventory

    Directory of Open Access Journals (Sweden)

    Javier Muzón

    2005-12-01

    Full Text Available RESUMEN. Se brinda un inventario preliminar de los insectos acuáticos de la Meseta del Somuncura y su área de influencia (Patagonia, Argentina realizado sobre la base de colecciones y registros previos de especies pertenecientes a los órdenes Ephemeroptera, Odonata, Hemiptera (Heteroptera, Trichoptera, Diptera (familias Ceratopogonidae, Culicidae y Psychodidae y Coleoptera. Se han relevado diversos tipos de ambientes en 14 localidades. El número de especies registrado asciende a 78, agrupadas en 51 géneros y 26 familias, de las cuales 33 se citan por primera vez del área. De los taxa registrados 83% de los géneros corresponden a grupos de amplia distribución (neotropicales, americanos o cosmopolitas, mientras que 41% de las especies presentan una distribución patagónica o andina.ABSTRACT. A preliminary inventory of the aquatic insects from the Somuncura plateau and its area of influence (Patagonia, Argentina is presented. It was done on the basis of the study of collections and previous records of species belonging to the orders Ephemeroptera, Odonata, Hemiptera (Heteroptera, Trichoptera, Diptera (families Ceratopogonidae, Culicidae, and Psychodidae and Coleoptera. Different kinds of environments were surveyed in 14 localities. Seventy eigth species grouped in 51 genera and 26 families were registered, and 33 species are new records for the area. Eighty three % of the registered genera are widely distributed (neotropical, american or cosmopolitan, while 41 % of the species exhibit patagonic or andean distribution.

  15. Development and evaluation of real-time PCR assays for bloodmeal identification in Culicoides midges.

    Science.gov (United States)

    VAN DER Saag, M R; Gu, X; Ward, M P; Kirkland, P D

    2016-06-01

    Culicoides (Diptera: Ceratopogonidae) midges are the biological vectors of a number of arboviruses of veterinary importance. However, knowledge relating to the basic biology of some species, including their host-feeding preferences, is limited. Identification of host-feeding preferences in haematophagous insects can help to elucidate the transmission dynamics of the arboviruses they may transmit. In this study, a series of semi-quantitative real-time polymerase chain reaction (qPCR) assays to identify the vertebrate host sources of bloodmeals of Culicoides midges was developed. Two pan-reactive species group and seven species-specific qPCR assays were developed and evaluated. The assays are quick to perform and less expensive than nucleic acid sequencing of bloodmeals. Using these assays, it was possible to rapidly test nearly 700 blood-fed midges of various species from several geographic locations in Australia. PMID:26854008

  16. Evaluation of in vitro methods for assessment of infection of Australian Culicoides spp. with bluetongue viruses.

    Science.gov (United States)

    Van der Saag, Matthew; Nicholas, Adrian; Ward, Michael; Kirkland, Peter

    2015-01-01

    Biting midges from the genus Culicoides (Diptera: Ceratopogonidae) are the vectors of several globally important arboviruses that affect livestock. These include orbiviruses from the bluetongue virus (BTV) and African horse sickness virus (AHSV) groups and members of the Simbu serogroup of orthobunyaviruses, such as the recently emerged Schmallenberg virus. In this article, the authors evaluate several methods for feeding wild‑caught Australian Culicoides on BTV infected preparations of blood and sucrose. Feeding Culicoides on the membrane of embryonated chicken eggs was identified as the preferred feeding method. Although, cotton wool pads soaked in either virus‑infected blood or virus‑sucrose mixtures were also successful. A non‑destructive nucleic acid extraction technique for the detection of viral RNA in Culicoides was also evaluated as it allows for readily differentiating infected from non‑infected Culicoides. PMID:26741248

  17. Culicoides biting midges at the National Zoological Gardens of South Africa : research communication

    Directory of Open Access Journals (Sweden)

    K. Labuschagne

    2007-09-01

    Full Text Available Culicoides biting midges (Diptera: Ceratopogonidae are responsible for the transmission of a large number of pathogens to livestock and wild animals. In this study the presence of the genus, using light traps based at four different sites within the National Zoological Gardens of South Africa, was investigated during 2002-2004. In total, 37 species were recorded, including large numbers of Culicoides imicola Kieffer, 1913, which is responsible for the transmission of economically important arboviruses in South Africa, Europe, Middle and Far East. These results are discussed with reference to the wider Culicoides fauna in the Onderstepoort area of South Africa, their vector competence as well as biosecurity at the National Zoological Gardens.

  18. Insects as vectors: systematics and biology.

    Science.gov (United States)

    Rodhain, F

    2015-04-01

    Among the many complex relationships between insects and microorganisms such as viruses, bacteria and parasites, some have resulted in the establishment of biological systems within which the insects act as a biological vector for infectious agents. It is therefore advisable to understand the identity and biology of these vectors in depth, in order to define procedures for epidemiological surveillance and anti-vector control. The following are successively reviewed in this article: Anoplura (lice), Siphonaptera (fleas), Heteroptera (bugs: Cimicidae, Triatoma, Belostomatidae), Psychodidae (sandflies), Simuliidae (black flies), Ceratopogonidae (biting midges), Culicidae (mosquitoes), Tabanidae (horseflies) and Muscidae (tsetse flies, stable flies and pupipara). The authors provide a rapid overview of the morphology, systematics, development cycle and bio-ecology of each of these groups of vectors. Finally, their medical and veterinary importance is briefly reviewed.

  19. Conteúdo dos criadouros larvais e comportamento de adultos de Toxorhynchites (Lynchiella haemorrhoidalis haemorrhoidalis (Fabricius (Diptera, Culicidae numa floresta de terra-firme da Amazônia central Larval breeding site contents and adult behavior of toxorhynchites (Lynchiella haemorrhoidalis haemorrhoidalis (Fabricius (Diptera, Culicidae in an upland forest of the central amazon

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    Rosa Sá Gomes Hutchings

    1995-01-01

    Full Text Available The natural breeding sites of Toxorhynchites (Lynchiella haemorrhoidalis haemorihoidalis (Fabricius, 1794, in two study areas, were sampled monthly, during a period of one year, in an upland "terra-firme" forest of the Central Amazon. These natural breeding sites, consisting of water filled palm bracts on the ground, contained invertobrates and vertebrates along with palm inflorescences, leaves and twigs. The inhabitants of the non-submersed area of the bracts include Diplopoda, Acarina, Araneae, Pseudoscorpiones, Isopoda, Blattodea, Coleoptera (Carabidae, Curculionidae, Scolytidae, Staphilinidae. Collembola, Dermaptera, Diptera (Cecidomyidae, Drosophilidae, Mycetophilidae, Tipulidae, Hemiptera, Hymenoptera and Trichoptera. The submersed areas of the bracts were inhabited by Oligochaeta, Coleoptera (Dysticidae, Helodidae, Histeridae, Hydrophilidae, Limnebiidae, Diptera (Ceratopogonidae, Chirononiidae, Culicidae, Psychodidae, Stratiomyidae, Syrphidae. Odonata, along with immature Dendrobatidae e Hylidae. The ovipositing, resting and feeding behaviors of T. h. haemorrhoidalis adults are described.

  20. MACROZOOBENTHIC COMMUNITIES STRUCTURE CHARACTERISTIC OF CERTAIN TRIBUTARIES OF THE SIRET RIVER FROM HARGHITA, MARAMUREŞ AND VRANCEA MOUNTAINS AND MOLDOVEI PLATEAU

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    Elena-Andreea GHIBUŞI

    2012-01-01

    Full Text Available 35 qualitative macrozoobentonic samples were collected in 2011 from many Siret river tributaries coming from the Harghita Mountains (5 stations, Maramureş Mountains (14 stations, Moldavian Plateau (4 stations and Vrancea Mountains (12 stations. Laboratory analysis of samples revealed the existence of the following 15 groups of benthic invertebrates: Ephemeroptera, Plecoptera, Trichoptera, Oligochaeta, Diptera (Chironomidae, Simuliidae, Ceratopogonidae, Limoniidae, Gastropoda, Bivalva, Coleoptera, Acarina, Odonata, Hirudinea, Isopoda, Heteroptera, Turbellariata and Collembola. Groups that have the highest frequencies were mayflies and dipterans (each with a frequency of 97.1%, followed by caddisflies (80%, amphipods (68.6%, oligochaetes (57.1% and stoneflies (54.3%. Presence of sensitive groups to water quality degradation (Ephemeroptera, Trichoptera and Plecoptera with high frequency shows good quality water at most stations investigated.

  1. Insects as vectors: systematics and biology.

    Science.gov (United States)

    Rodhain, F

    2015-04-01

    Among the many complex relationships between insects and microorganisms such as viruses, bacteria and parasites, some have resulted in the establishment of biological systems within which the insects act as a biological vector for infectious agents. It is therefore advisable to understand the identity and biology of these vectors in depth, in order to define procedures for epidemiological surveillance and anti-vector control. The following are successively reviewed in this article: Anoplura (lice), Siphonaptera (fleas), Heteroptera (bugs: Cimicidae, Triatoma, Belostomatidae), Psychodidae (sandflies), Simuliidae (black flies), Ceratopogonidae (biting midges), Culicidae (mosquitoes), Tabanidae (horseflies) and Muscidae (tsetse flies, stable flies and pupipara). The authors provide a rapid overview of the morphology, systematics, development cycle and bio-ecology of each of these groups of vectors. Finally, their medical and veterinary importance is briefly reviewed. PMID:26470450

  2. Seasonality, parity rates and transmission indices of Mansonella ozzardi (Manson (Nematoda: Onchocercidae by Cerqueirellum argentiscutum (Shelley & Luna Dias (Diptera: Simulidae in a lower Solimões River community, Amazonas, Brazil Sazonalidade, taxa de paridade e índices de transmissão de Mansonella ozzardi (Manson (Nematoda: Onchocercidae por Cerqueirellum argentiscutum (Shelley & Luna Dias (Diptera: Simuliidae em uma comunidade do baixo rio Solimões, Amazonas, Brasil

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    Jansen Fernandes de Medeiros

    2004-01-01

    Full Text Available Mansonella ozzardi is transmitted by two dipterian families, Ceratopogonidae (midges and Simuliidae (black flies. In Brazil, black flies are vectors for this filariasis until now. In this paper, we determined the seasonality, parity capacity and parasitic infection rate of Cerqueirellum argentiscutum. The work was carried out in the Porto Japão community, Lower Solimões River, Amazonas, Brazil. Results show that the black flies were more abundant during the rainy season (from December to May. The number of parous flies was higher in every sampling during the course of year. Monthly Biting Rate (MBR1 123742.00, MBR2 86701.50 was high, although Parasitic Infection Rate (PIR1 0.06, PIR2 0.08 and Annual Transmission Potential (ATP 7.25 were low in numbers.Mansonella ozzardi é transmitida por dois grupos distintos de insetos, Ceratopogonidae e Simuliidae. No Brasil, os simulídeos são os vetores dessa filariose. Neste trabalho, o objetivo foi determinar a sazonalidade, a paridade e a taxa de infecção parasitária de Cerqueirellum argentiscutum. O experimento foi realizado na comunidade Porto do Japão, Baixo rio Solimões, Amazonas, Brasil. Os simulídeos foram mais abundantes no período de chuva (dezembro a maio. Em todos os meses de coletas o número de paríparas foi maior. A taxa mensal de picada (TMP1 123742.00, TMP2 86701.50 foi elevada, já a taxa de infecção parasitária (TIP1 0.06, TIP2 0.08 e o potencial de transmissão anual (PTA 7.25 foram considerados baixos.

  3. Macroinvertebrates associated with bryophyta in a first-order Atlantic Forest stream

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    Beatriz F. J. V. Rosa

    2011-06-01

    Full Text Available This study describes the composition and structure of the benthic community associated with bryophytes in a first-order stream, located in a biological reserve of the Atlantic Forest, during two seasons. During three months of the dry season of 2007 and three months of the rainy season of 2008, samples of bryophytes attached to stones were collected randomly, along a 100 m stream reach. The structure of the community was analyzed through the mean density of individuals, Shannon's diversity index, Pielou's evenness, family richness, dominance index, and the percentage of Ephemeroptera, Plecoptera and Trichoptera (% EPT. Chironomidae larvae were dominant in the two periods of study, followed by Ceratopogonidae in the rainy season, and Naididae in the dry season. The orders EPT contributed 14 families. The results showed that bryophytes constitute suitable habitat which is able to shelter an abundant and diversified benthic fauna in a small extension of the stream. This habitat provides refuge during spates, and thus minimizes downstream transport of the macroinvertebrate fauna.

  4. In situ effects of titanium dioxide nanoparticles on community structure of freshwater benthic macroinvertebrates.

    Science.gov (United States)

    Jovanović, Boris; Milošević, Djuradj; Piperac, Milica Stojković; Savić, Ana

    2016-06-01

    For the first time in the current literature, the effect of titanium dioxide (TiO2) nanoparticles on the community structure of macroinvertebrates has been investigated in situ. Macroinvertebrates were exposed for 100 days to an environmentally relevant concentration of TiO2 nanoparticles, 25 mg kg(-1) in sediment. Czekanowski's index was 0.61, meaning 39% of the macroinvertebrate community structure was affected by the TiO2 treatment. Non-metric multidimensional scaling (NMDS) visualized the qualitative and quantitative variability of macroinvertebrates at the community level among all samples. A distance-based permutational multivariate analysis of variance (PERMANOVA) revealed the significant effect of TiO2 on the macroinvertebrate community structure. The indicator value analysis showed that the relative frequency and abundance of Planorbarius corneus and Radix labiata were significantly lower in the TiO2 treatment than in the control. Meanwhile, Ceratopogonidae, showed a significantly higher relative frequency and abundance in the TiO2 treatment than in the control.

  5. Identification of human-derived volatile chemicals that interfere with attraction of the Scottish biting midge and their potential use as repellents.

    Science.gov (United States)

    Logan, James G; Seal, Nicola J; Cook, James I; Stanczyk, Nina M; Birkett, Michael A; Clark, Suzanne J; Gezan, Salvador A; Wadhams, Lester J; Pickett, John A; Mordue, A Jennifer

    2009-03-01

    The Scottish biting midge, Culicoides impunctatus (Diptera: Ceratopogonidae), is a major pest in Scotland, causing a significant impact to the Scottish tourist and forestry industries. C. impunctatus is a generalist feeder, preferring to feed on large mammals, and is notorious for its attacks on humans. Until now, there was anecdotal evidence for differential attraction of female host-seeking C. impunctatus to individual human hosts, and the mechanism for this phenomenon was unknown. Using extracts of human odor collected by air entrainment, electroantennogram recordings to identify the physiologically active components, followed by behavioral assays, we show, for the first time, the differential attraction of female C. impunctatus to human odors and the chemical basis for this phenomenon. Certain chemicals, found in greater amounts in extracts that cause low attractiveness to midges, elicit a repellent effect in laboratory assays and repellency trials in the field. Differences in the production of these natural human-derived compounds could help to explain differential "attractiveness" between different human hosts. A mixture of two compounds in particular, 6-methyl-5-hepten-2-one and geranylacetone [(E)-6,10-dimethylundeca-5,9-dien-2-one], showed significant repellency (87, 77.4, 74.2, and 31.6% at hours 0, 1, 2, and 3, respectively) in the field and have the potential to be developed as novel repellents. PMID:19351071

  6. Macroinvertebrate communities associated with macrophyte habitats in a tropical man-made lake (Lake Taabo, Côte d’Ivoire

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    Kouamé M. K.

    2011-02-01

    Full Text Available An ecological study was done on Lake Taabo with the main objective of characterising macroinvertebrate communities associated with the microhabitats created mainly by Eichhornia crassipes and other littoral native macrophytes. We sampled organisms in patches of those aquatic macrophytes. Also, some abiotic variables (temperature, transparency, turbidity, pH, TDS, conductivity, dissolved oxygen, NH$_{4}^{+}$+4, NO$_{3}^{-}$−3, NO$_{2}^{-}$−2, PO$_{4}^{3-}$3−4 and SiO$_{2}^{-}$−2 were measured. Overall, forty-three taxa of macroinvertebrates were identified. Ten of them were exclusively associated with water hyacinth while five were only associated with littoral macrophytes. Macroinvertebrate taxa with some of the highest family richness were Gastropoda, Coleoptera, Heteroptera, Odonata and Diptera. The taxon with highest density in both microhabitats was Chironomidae. Although higher values of taxonomic richness (Rs, the Shannon index (H′ and evenness (J were obtained with the water hyacinth habitat, significant differences between the two microhabitats were not observed. Canonical Correspondence Analysis revealed that samples of E. crassipes collected in the dry season were characterised by Gastropoda and Odonata, as well as higher values of transparency and ammonia-nitrogen. Baetidae, Hydrophilidae, Chironomidae, Ceratopogonidae, Coenagrionidae, Naucoridae and Ostracoda were most abundant in both E. crassipes and littoral macrophyte habitats during the rainy season. This season was characterised by higher levels of nitrates and conductivity.

  7. In situ effects of titanium dioxide nanoparticles on community structure of freshwater benthic macroinvertebrates.

    Science.gov (United States)

    Jovanović, Boris; Milošević, Djuradj; Piperac, Milica Stojković; Savić, Ana

    2016-06-01

    For the first time in the current literature, the effect of titanium dioxide (TiO2) nanoparticles on the community structure of macroinvertebrates has been investigated in situ. Macroinvertebrates were exposed for 100 days to an environmentally relevant concentration of TiO2 nanoparticles, 25 mg kg(-1) in sediment. Czekanowski's index was 0.61, meaning 39% of the macroinvertebrate community structure was affected by the TiO2 treatment. Non-metric multidimensional scaling (NMDS) visualized the qualitative and quantitative variability of macroinvertebrates at the community level among all samples. A distance-based permutational multivariate analysis of variance (PERMANOVA) revealed the significant effect of TiO2 on the macroinvertebrate community structure. The indicator value analysis showed that the relative frequency and abundance of Planorbarius corneus and Radix labiata were significantly lower in the TiO2 treatment than in the control. Meanwhile, Ceratopogonidae, showed a significantly higher relative frequency and abundance in the TiO2 treatment than in the control. PMID:26924756

  8. Assessment of the repellent effect of citronella and lemon eucalyptus oil against South African Culicoides species

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    Gert J. Venter

    2014-02-01

    Full Text Available The use of insect repellents to reduce the attack rate of Culicoides species (Diptera:Ceratopogonidae should form part of an integrated control programme to combat Africanhorse sickness and other diseases transmitted by these blood-feeding midges. In the presentstudy the repellent effects of a commercially available mosquito repellent, a combinationof citronella and lemon eucalyptus oils, on Culicoides midges was determined. The numberof midges collected with two 220 V Onderstepoort traps fitted with 8 W 23 cm white lighttubes and baited with peel-stick patches, each containing 40 mg of active ingredient, wascompared with that of two unbaited traps. Two trials were conducted and in each trial thefour traps were rotated in two replicates of a 4 x 4 randomised Latin square design. Althoughmore midges were collected in the baited traps, the mean number in the baited and unbaitedtraps was not significantly different. This mosquito repellent did not influence either thespecies composition or the physiological groups of Culicoides imicola Kieffer. The highermean numbers in the baited traps, although not statistically significant, may indicate that thismosquito repellent might even attract Culicoides midges under certain conditions.

  9. Molecular Taxonomy of a Phantom Midge Species (Chaoborus flavicans in Korea

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    Haein An

    2012-01-01

    Full Text Available The larvae of Chaoborus are widely distributed in lakes, ponds, and reservoirs. These omnivorous Chaoborus larvae are crucial predators and play a role in structuring zooplankton communities, especially for small-sized prey. Larvae of Chaoborus are commonly known to produce predator-induced polyphenism in Daphnia sp. Nevertheless, their taxonomy and molecular phylogeny are very poorly understood. As a fundamental study for understanding the role of Chaoborus in predator-prey interactions in a freshwater ecosystem, the molecular identification and phylogenetic relationship of Chaoborus were analyzed in this study. A molecular comparison based on partial mitochondrial cytochrome oxidase I (COI between species in Chaoborus was carried out for the identification of Chaoborus larvae collected from 2 localities in Korea. According to the results, the Chaoborus species examined here was identified as C. flavicans, which is a lake-dwelling species. Furthermore, partial mitochondrial genome including COI, COII, ATP6, ATP8, COIII, and ND3 were also newly sequenced from the species and concatenated 5 gene sequences excluding ATP8 with another 9 dipteran species were compared to examine phylogenetic relationships of C. flavicans. The results suggested that Chaoborus was more related to the Ceratopogonidae than to the Culicidae. Further analysis based on complete mitochondrial DNA sequences and nuclear gene sequences will provide a more robust validation of the phylogenetic relationships of Chaoborus within dipteran lineages.

  10. Vector competence of Culicoides for arboviruses: three major periods of research, their influence on current studies and future directions.

    Science.gov (United States)

    Carpenter, S; Veronesi, E; Mullens, B; Venter, G

    2015-04-01

    The spectacular and unprecedented outbreaks of bluetongue virus (BTV) that have occurred in Europe since 1998 have led to increased interest in those factors that determine competence of Culicoides biting midges (Diptera: Ceratopogonidae) for arboviruses. In this review the authors critically examine three major periods of research into the biological transmission by Culicoides of two economically important arboviruses ofthefamily Reoviridae: African horse sicknessvirus (AHSV) and BTV. First they examine early studies, largely conducted in southern Africa, that played a key role in initially implicating Culicoides as agents of AHSV and BTV transmission. Then they examine advances in understanding made following the establishment of colonies of the BTV vector species Culicoides sonorensis, which have largely shaped our current understanding of BTV and AHSV transmission. They then consider attempts in recent years to implicate vectors of BTV in the European Union during what has become the most economically damaging series of outbreaks in recorded history. In some cases the origin of these outbreaks was uncertain and unexpected, particularly in northern Europe, where BTV had not previously occurred. Limitations imposed on studies of vector competence by the biology of Culicoides are then discussed, along with advances in the technologies now available and the logistics of working upon agents requiring biosecure containment outside their endemic range. Finally, the authors suggest areas that have either been poorly addressed to date or entirely ignored and ways in which studies could be conducted to provide standardised data for comparison worldwide.

  11. Detection of African horse sickness virus in Culicoides imicola pools using RT-qPCR.

    Science.gov (United States)

    de Waal, Tania; Liebenberg, Danica; Venter, Gert J; Mienie, Charlotte Ms; van Hamburg, Huib

    2016-06-01

    African horse sickness (AHS) is an infectious, non-contagious arthropod-borne disease of equids, caused by the African horse sickness virus (AHSV), an orbivirus of the Reoviridae family. It is endemic in sub-Saharan Africa and thought to be the most lethal viral disease of horses. This study focused on detection of AHSV in Culicoides imicola (Diptera: Ceratopogonidae) pools by the application of a RT-qPCR. Midges were fed on AHSV-infected blood. A single blood-engorged female was allocated to pools of unfed nulliparous female midges. Pool sizes varied from 1 to 200. RNA was extracted and prepared for RT-qPCR. The virus was successfully detected and the optimal pool size for the limit of detection of the virus was determined at a range between 1 to 25. Results from this investigation highlight the need for a standardized protocol for AHSV investigation in Culicoides midges especially for comparison among different studies and for the determination of infection rate. PMID:27232141

  12. The suitability of the Triple trap for the collection of South African livestock-associated Culicoides species

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    Gert J. Venter

    2013-02-01

    Full Text Available The relatively large number of Culicoides midges (Diptera: Ceratopogonidae that can be collected with a light trap makes it the most widely used tool for this purpose. However, the majority of these traps were originally designed for collecting mosquitoes. The evaluation and improvement of traps to increase their effectiveness in collecting Culicoides midges will unavoidably form part of research on these insects. In the present study the efficiency of the Triple trap for collecting livestock-associated Culicoides midges was compared with that of the Onderstepoort 220 V, the BG-sentinel and the mini-CDC traps. A unique feature of the Triple trap is that selected surfaces are coated with TiO2 (titanium dioxide which, in the presence of ultra violet light, acts as a photo-catalyser to produce CO2, which in turn may attract blood-feeding insects. Overall, the Onderstepoort trap collected significantly higher numbers of midges than the others. Relative efficiency varied between different occasions and under some conditions, for example periods with low midge abundance during the winter, the mean numbers collected with the Triple trap did not differ significantly from those of the Onderstepoort or BG-sentinel traps. By replacing the collection chamber of the Triple trap with a sock and beaker, similar to that of the Onderstepoort trap, it can effectively be used for the collection of Culicoides midges.

  13. Sugar-feeding status alters biting midge photoattraction.

    Science.gov (United States)

    Snyder, D; Cernicchiaro, N; Cohnstaedt, L W

    2016-03-01

    The biting midge Culicoides sonorensis Wirth and Jones (Diptera: Ceratopogonidae) transmits pathogens to both livestock and wildlife. Biting midge surveillance relies heavily on light traps for collection; however, little is known about the light spectra preferences of C. sonorensis midges. A light assay arena was constructed and light-emitting diodes (LEDs) of various light spectra were used as light sources to evaluate midge photoattraction. A comparison of responses to light spectra indicated the highest proportions of C. sonorensis were attracted to ultraviolet (UV) light and that midges differentiated 10-nm differences in wavelength. Stronger intensities of UV light resulted in greater attraction. Midges exhibited both sugar-seeking and escape behaviours under different conditions of sugar supplementation before and during the experiment. These behaviours occurred with lights of 355 nm and 365 nm in wavelength. Based on the results of this study, the attraction of C. sonorensis to light traps can be improved through the use of bright LEDs at 355 nm or 365 nm. PMID:26555011

  14. The dying of the light: crepuscular activity in Culicoides and impact on light trap efficacy at temperate latitudes.

    Science.gov (United States)

    Meiswinkel, R; Elbers, A R W

    2016-03-01

    The light trap is the tool of choice for conducting large-scale Culicoides (Diptera: Ceratopogonidae) vector surveillance programmes. Its efficacy is in doubt, however. To assess this, hourly changes in Culicoides activity over the 24-h diel were determined comparatively by way of light trapping and aerial sweeping, and correlated against light intensity. In the Netherlands, sweeping around cattle at pasture revealed that, in early summer, Culicoides are active throughout the diel, and that their abundance peaks during the crepuscular period and falls to a low during the brightest hours of the day. By contrast, the light trap was able to accumulate Culicoides only at night (i.e. after illuminance levels had dropped to 0 lux and midge activity had begun to decline). Although Culicoides chiopterus and species of the Culicoides obsoletus complex were similarly abundant around livestock, they differed critically in their hours of peak activity, being largely diurnal and nocturnal, respectively. This polarity helps to explain why, routinely, the C. obsoletus complex dominates light trap collections and C. chiopterus does not. Inability to accumulate Culicoides at light intensity levels above 0 lux means that, at ever-higher latitudes, particularly beyond 45° N, the progressive northward lengthening of the twilight period will have an increasingly adverse impact upon the efficacy of the light trap as a vector surveillance tool. PMID:26555116

  15. Trap placement and attractant choice affect capture and create sex and parity biases in collections of the biting midge, Culicoides sonorensis.

    Science.gov (United States)

    McDermott, E G; Mayo, C E; Gerry, A C; Mullens, B A

    2016-09-01

    Culicoides sonorensis Wirth & Jones (Diptera: Ceratopogonidae) is the primary North American vector of bluetongue virus (BTV), which can cause high morbidity and mortality in ruminant livestock or wildlife. Worldwide, most Culicoides surveillance relies on light (usually UV) traps typically placed near animals or larval development sites. However, the trapping method can cause sex, species and parity biases in collections. We collected C. sonorensis from three dairies in California using suction traps baited with CO2 , UV light or CO2  + UV placed near animals, wastewater ponds, or in fields. Higher numbers of parous females were collected using CO2  + UV traps, although this difference was only significant on one dairy. UV traps were poor at collecting nulliparous females, but the addition of UV to a trap increased the abundance of males in a collection. Traps set in open fields collected significantly higher numbers of males and females than in either of the other two locations. In some cases, there was a significant interaction between the trap type and site. We discuss the limitations of traditional trapping methodologies for C. sonorensis and make suggestions for vector surveillance. PMID:27257164

  16. Insecticidal sugar baits for adult biting midges.

    Science.gov (United States)

    Snyder, D; Cernicchiaro, N; Allan, S A; Cohnstaedt, L W

    2016-06-01

    The mixing of an insecticide with sugar solution creates an oral toxin or insecticidal sugar bait (ISB) useful for reducing adult insect populations. The ability of ISBs to kill the biting midge Culicoides sonorensis Wirth and Jones (Diptera: Ceratopogonidae), a vector of bluetongue virus, epizootic hemorrhagic disease and vesicular stomatitis viruses, was tested. The commercial insecticide formulations (percentage active ingredient) tested included bifenthrin, cyfluthrin, deltamethrin, permethrin, dinotefuran, imidacloprid, thiamethoxam and spinosad. Mortality rates were determined for various concentrations of commercial formulations (0.01, 0.05, 0.1, 1, 2 and 3%) and observed at 1, 4, 10 and 24 h post-exposure to the ISB. In the first set of assays, laboratory-reared midges were fed sugar ad libitum and then exposed to insecticide-treated sugar solutions to measure mortality. The second assay assessed competitive feeding: midges were provided with a control sugar solution (10% sucrose) in one vial, and a sugar and insecticide solution in another. Pyrethroid treatments resulted in the greatest mortality in the first hour at the lowest concentrations and spinosad consumption resulted in the least mortality. Biting midges were not deterred from feeding on the 1% ISB solutions despite the presence of an insecticide-free alternative source of sugar. PMID:26789534

  17. Comparing the effect of modeled climatic variables on the distribution of African horse sickness in South Africa and Namibia.

    Science.gov (United States)

    Liebenberg, Danica; van Hamburg, Huib; Piketh, Stuart; Burger, Roelof

    2015-12-01

    Africa horse sickness (AHS) is a lethal disease of horses with a seasonal occurrence that is influenced by environmental conditions that favor the development of Culicoides midges (Diptera: Ceratopogonidae). This study compared and evaluated the relationship of various modeled climatic variables with the distribution and abundance of AHS in South Africa and Namibia. A comprehensive literature review of the historical AHS reported data collected from the Windhoek archives as well as annual reports from the Directorate of Veterinary services in Namibia were conducted. South African AHS reported data were collected from the South African Department of Agriculture, Forestry, and Fisheries. Daily climatic data were extracted for the time period 1993-2011 from the ERA-interim re-analysis dataset. The principal component analysis of the complete dataset indicated a significant statistical difference between Namibia and South Africa for the various climate variables and the outbreaks of AHS. The most influential parameters in the distribution of AHS included humidity, precipitation, evaporation, and minimum temperature. In South Africa, temperature had the most significant effect on the outbreaks of AHS, whereas in Namibia, humidity and precipitation were the main drivers. The maximum AHS cases in South Africa occurred at temperatures of 20-22° C and relative humidity between 50-70%. Furthermore, anthropogenic effects must be taken into account when trying to understand the distribution of AHS. PMID:26611969

  18. Characterization of Viral Communities of Biting Midges and Identification of Novel Thogotovirus Species and Rhabdovirus Genus.

    Science.gov (United States)

    Temmam, Sarah; Monteil-Bouchard, Sonia; Robert, Catherine; Baudoin, Jean-Pierre; Sambou, Masse; Aubadie-Ladrix, Maxence; Labas, Noémie; Raoult, Didier; Mediannikov, Oleg; Desnues, Christelle

    2016-03-01

    More than two thirds of emerging viruses are of zoonotic origin, and among them RNA viruses represent the majority. Ceratopogonidae (genus Culicoides) are well-known vectors of several viruses responsible for epizooties (bluetongue, epizootic haemorrhagic disease, etc.). They are also vectors of the only known virus infecting humans: the Oropouche virus. Female midges usually feed on a variety of hosts, leading to possible transmission of emerging viruses from animals to humans. In this context, we report here the analysis of RNA viral communities of Senegalese biting midges using next-generation sequencing techniques as a preliminary step toward the identification of potential viral biohazards. Sequencing of the RNA virome of three pools of Culicoides revealed the presence of a significant diversity of viruses infecting plants, insects and mammals. Several novel viruses were detected, including a novel Thogotovirus species, related but genetically distant from previously described tick-borne thogotoviruses. Novel rhabdoviruses were also detected, possibly constituting a novel Rhabdoviridae genus, and putatively restricted to insects. Sequences related to the major viruses transmitted by Culicoides, i.e., African horse sickness, bluetongue and epizootic haemorrhagic disease viruses were also detected. This study highlights the interest in monitoring the emergence and circulation of zoonoses and epizooties using their arthropod vectors. PMID:26978389

  19. Predicting the abundance of African horse sickness vectors in South Africa using GIS and artificial neural networks

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    Sanet Eksteen

    2011-07-01

    Full Text Available African horse sickness (AHS is a disease that is endemic to sub-Saharan Africa and is caused by a virus potentially transmitted by a number of Culicoides species (Diptera: Ceratopogonidae including Culicoides imicola and Culicoides bolitinos. The strong association between outbreaks of AHS and the occurrence in abundance of these two Culicoides species has enabled researchers to develop models to predict potential outbreaks. A weakness of current models is their inability to determine the relationships that occur amongst the large number of variables potentially influencing the population density of the Culicoides species. It is this limitation that prompted the development of a predictive model with the capacity to make such determinations. The model proposed here combines a geographic information system (GIS with an artificial neural network (ANN. The overall accuracy of the ANN model is 83%, which is similar to other stand-alone GIS models. Our predictive model is made accessible to a wide range of practitioners by the accompanying C. imicola and C. bolitinos distribution maps, which facilitate the visualisation of the model’s predictions. The model also demonstrates how ANN can assist GIS in decision-making, especially where the data sets incorporate uncertainty or if the relationships between the variables are not yet known.

  20. Influence of carbon dioxide on numbers of Culicoides midges collected with suction light traps in South Africa.

    Science.gov (United States)

    Venter, G J; Boikanyo, S N B; Majatladi, D M; Morey, L

    2016-03-01

    To implement risk management against diseases transmitted by species of Culicoides Latreille, 1809 (Diptera: Ceratopogonidae), it is essential to identify all potential vectors. Light traps are the most commonly used tool for the collection of Culicoides midges. Given the indiscriminate artificial attraction of light, traps will collect all night-flying insects rather than only livestock-associated Culicoides midges. Factors that may increase the efficacy of traps, especially for livestock-associated Culicoides midges, require investigation. In the present study, results obtained with Centers for Disease Control (CDC) and Onderstepoort light traps baited with carbon dioxide (CO2 ) were compared with those of unbaited controls. Comparisons were made using two replicates of a 4 × 4 randomized Latin square design. With both trap types, the mean numbers of Culicoides midges collected in 16 baited traps were higher than those caught in 16 unbaited traps. Although exceptionally low numbers were collected with the CDC traps, the increases in the numbers and frequency of collection of Culicoides imicola Kieffer, 1913 were more pronounced in the CDC traps compared with the Onderstepoort traps. These results indicate that the addition of CO2 may increase the efficiency of these traps for the collection of C. imicola and other livestock-associated Culicoides species. PMID:26522279

  1. Emergence of Culicoides obsoletus group species from farm-associated habitats in Germany.

    Science.gov (United States)

    Steinke, S; Lühken, R; Balczun, C; Kiel, E

    2016-06-01

    Biting midges of the genus Culicoides (Diptera: Ceratopogonidae) may transmit several arboviruses to ruminant livestock. The species of the Obsoletus group are considered to be among the most important vectors of bluetongue virus (BTV) in northern Europe. As agricultural environments offer suitable habitats for the development of their immature stages, the emergence of adult Culicoides from potential breeding sites was investigated at 20 cattle farms throughout Germany in 2012 and 2013. In analyses of species-specific habitat preferences and relationships between Culicoides abundance in breeding substrates and their physicochemical characteristics, dungheaps emerged as the most important substrate for the development of Culicoides obsoletus sensu stricto (s.s.) (Meigen), whereas Culicoides chiopterus (Meigen) and Culicoides dewulfi Goetghebuer were generally restricted to cowpats. A decreasing pH value was associated with a higher abundance or a higher probability of observing these three species. Furthermore, the abundance of C. obsoletus s.s. was positively related to increasing moisture. Dungheaps were very productive breeding sites for this species and are therefore suggested as a target for potential control measures. PMID:26744290

  2. Schmallenberg virus in Germany 2011-2014: searching for the vectors.

    Science.gov (United States)

    Kameke, Daniela; Werner, Doreen; Hoffmann, Bernd; Lutz, Walburga; Kampen, Helge

    2016-02-01

    Following the emergence of Schmallenberg virus (SBV) in 2011, 21,397 culicoid biting midges (Diptera: Ceratopogonidae) from targeted and non-targeted sampling activities carried out during the summer months of 2011 to 2013 and in late 2014 in various regions in Germany were analyzed for the virus by real-time RT-PCR. While no SBV was found in biting midges collected during 2011 and 2013, 2 out of 334 pools including 20 and 22 non-engorged females of the Obsoletus complex sampled in 2012 tested positive for the SBV S-segment with C(t) values of 42.46 and 35.45. In addition, 673 black flies (Diptera: Simuliidae) captured during the same studies were screened for the presence of SBV and proved negative. In late autumn 2014, biting midges were collected again in a limited study in eastern Germany after some cases of SBV infection had occurred in a quarantine station for cattle. Due to the unfavorable seasonal weather conditions, only few specimens were caught, and these were also negative for SBV. The German experience suggests that biting midge collections launched only after an outbreak and are not locally targeted may be ineffective as to virus detection. It rather might be advisable to collect biting midges at sentinel farms on a permanent basis so to have material available to be examined in the case of a disease outbreak. PMID:26462800

  3. Structure and function of the cibarial armature in Simuliidae.

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    Reid, G D

    1994-07-01

    Cibarial armature morphology in adult female blackflies (Diptera: Simuliidae) is described using scanning electron microscopy. Three distinct types of armature are recognized, comprising those with teeth, e.g. Simulium ochraceum, S. ornatum, S. veracruzanum and S. vorax, those with spicules, e.g. Austrosimulium bancrofti, S. damnosum, S. exiguum, S. metallicum and S. neavei; and those lacking these projections, e.g. Prosimulium rufipes and S. lineatum. Whereas the armature is poorly developed in vectors of human onchocerciasis such as S. damnosum, S. exiguum, S. metallicum and S. neavei, the well-developed armature in S. ochraceum, S. veracruzanum and S. vorax does not prevent these species becoming infected with Onchocerca spp. (Nematoda: Onchocercidae). Hence the armature is not primarily a mechanism to counteract microfilaria superinfection. Since cibarial armatures are more developed in the haematophagous females than in the males of certain Families of flies, e.g. Ceratopogonidae, Culicidae, Phlebotominae and Simuliidae in the sub-order Nematocera, evidently the armature has evolved in response to the blood-feeding habit. As the suction of imbibed blood by the cibarial pump may require a valve mechanism to prevent back-flow, it is suggested that the armature is primarily for this purpose. Secondarily, the cibarial armature presents a damaging barrier against ingested microfilariae.

  4. Composition and Longitudinal Patterns of Aquatic Insect Emergence in Small Rivers of Palawan Island, the Philippines

    Science.gov (United States)

    Freitag, Hendrik

    2004-09-01

    This study presents the first emergence trap samples from streams in the Philippines and Greater Sunda. Aquatic insect emergence from two small rivers and longitudinal patterns including estuaries are compared. A decline of total emergence towards estuaries was observed, affecting all major orders. Diptera, namely Chironomidae, dominated all sites. High abundances in Ceratopogonidae, Odonata, and Coleoptera were found, compared to other emergence studies from tropical and temperate latitudes. Ephemeroptera displayed a highly variable contribution to the emergence from Palawan as well as in other comparative studies either supported by the appropriate conditions for certain functional groups or limited by environmental variables such as pH. Trichoptera are likely to tolerate a wider range of environmental conditions and they are consequently able to fill further niches where Ephemeroptera are under-represented. Except for scarce abundances of Plecoptera observed in this and other studies from the tropics, no substantial differences in emergence composition at order level existed between temperate and tropical rivers, however, with a remarkable local variation. Components of riparian and non-aquatic insects and non-emergent fauna contributing to the collections are discussed based on trap features. (

  5. Influence of biotic and abiotic factors on the distribution and abundance of Culicoides imicola and the Obsoletus Complex in Italy.

    Science.gov (United States)

    Conte, A; Goffredo, M; Ippoliti, C; Meiswinkel, R

    2007-12-25

    Culicoides imicola Kieffer (Culicoides, Diptera: Ceratopogonidae) is the principal vector of bluetongue virus (BTV) to ruminant livestock in southern Europe. The secondary potential vectors are Culicoides obsoletus (Meigen) and Culicoides scoticus Downes and Kettle of the Obsoletus Complex, Culicoides pulicaris (Linnaeus) of the Pulicaris Complex and Culicoides dewulfi Goetghebuer of the subgenus Avaritia Fox. Between 2000 and 2004 >38,000 light-trap collections were made for Culicoides across Italy including the islands of Sardinia and Sicily. Mapping of the 100 largest collections of C. imicola and of the Obsoletus Complex showed them to be disjunct overlapping in only 2% of the 200 municipalities selected. For each municipality the average values were calculated for minimum temperature, aridity index, altitude, terrain slope, normalised difference vegetation index (NDVI) and percentage forest cover. A factor analysis identified two principal factors ('biotic' and 'abiotic') and explained 84% of the total variability; a discriminant analysis classified correctly 87.5% of the observations. The results indicate adult populations of C. imicola to occur in more sparsely vegetated habitats that are exposed to full sunlight, whereas species of the Obsoletus Complex favour a more shaded habitat, with increased green leaf density. Heliophily and umbrophily, by shortening or lengthening the respective adult life cycles of these two vectors, will likely impact on the ability of each to transmit BTV and is discussed in the light of the current outbreak of BTV across the Mediterranean Basin. PMID:17997043

  6. The effect of high frequency sound on Culicoides numbers collected with suction light traps

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    Gert J. Venter

    2012-04-01

    Full Text Available Culicoides midges (Diptera: Ceratopogonidae, are involved in the transmission of various pathogens that cause important diseases of livestock worldwide. The use of insect repellents to reduce the attack rate of these insects on livestock could play an important role as part of an integrated control programme against diseases transmitted by these midges. The objective of this study was to determine whether high frequency sound has any repellent effect on Culicoides midges. The number of midges collected with 220 V Onderstepoort white light traps fitted with electronic mosquito repellents (EMRs, emitting 5-20 KHz multi-frequency sound waves, was compared with that of two untreated traps. Treatments were rotated in two replicates of a 4 x 4 randomised Latin square design. Although fewer midges were collected in the two traps fitted with EMRs, the average number collected over eight consecutive nights was not significantly different. The EMRs also had no influence on any of the physiological groups of Culicoides imicola Kieffer or the species composition of the Culicoides population as determined with light traps. The results indicate that high frequency sound has no repellent effect on Culicoides midges. There is therefore no evidence to support their promotion or use in the protection of animals against pathogens transmitted by Culicoides midges.

  7. The repellent effect of organic fatty acids on Culicoides midges as determined with suction light traps in South Africa.

    Science.gov (United States)

    Venter, G J; Labuschagne, K; Boikanyo, S N B; Morey, L; Snyman, M G

    2011-09-27

    The efficacy of a 15% (w/w) mixture of octanoic, nonanoic and decanoic acids in light mineral oil to repel Culicoides biting midges (Diptera; Ceratopogonidae) was determined in three replicates of a 4 × 4 Latin square design under South African field conditions. The fatty acids were applied to ± 0.07 m(2) polyester meshes with a mesh size 2-3mm fitted to 220 V 8 W Onderstepoort downdraught light traps. To reduce the relatively strong attraction of the light trap, the black light tubes in the Onderstepoort trap were replaced with 8 W 23 cm white light tubes. The traps were operating overnight next to cattle. Two traps treated with the mixture of fatty acids collected 1.7 times fewer midges than two untreated traps. Although this mixture of fatty acids had shown a repellent effect against a number of blood-feeding insects this is the first indication that it also has a significant repellent effect against Culicoides species and especially Culicoides (Avaritia) imicola Kieffer when applied to polyester mesh. PMID:21592665

  8. Efficacy of aerial spray applications using fuselage booms on Air Force C-130H aircraft against mosquitoes and biting midges.

    Science.gov (United States)

    Breidenbaugh, Mark S; Haagsma, Karl A; Wojcik, George M; De Szalay, Ferenc A

    2009-12-01

    The effectiveness of a novel fuselage boom configuration was tested with flat-fan nozzles on U.S. Air Force C-130H aircraft to create ultra-low volume sprays to control mosquitoes (Culicidae) and biting midges (Ceratopogonidae). The mortality of mosquitoes and biting midges in bioassay cages and natural populations, using the organophosphate adulticide, naled, was measured. Mosquitoes in bioassay cages had 100% mortality at 639 m downwind in all single-pass spray trials, and most trials had >90% mortality up to 1491 m downwind. Mosquito mortality was negatively correlated with distance from the spray release point (r2 = 0.38, P 90%). In wide-area operational applications, numbers of mosquitoes from natural populations 1 wk postspray were 83% (range 55%-95%), lower than prespray numbers (P < 0.05). Biting midge numbers were reduced by 86% (range 53%-97%) on average (P = 0.051) after 7 days. The results of these field trials indicate that the fuselage boom configuration on C-130H aircraft are an effective method to conduct large-scale aerial sprays during military operations and public health emergencies. PMID:20099594

  9. Preemptive Circular Defence of Immature Insects: Definition and Occurrences of Cycloalexy Revisited

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    Guillaume J. Dury

    2014-01-01

    Full Text Available Cycloalexy was coined by Vasconcellos-Neto and Jolivet in 1988 and further defined by Jolivet and collaborators in 1990 in reference to a specific type of circular defence. The term has been applied to numerous organisms, including adult insects, nymphs, and even vertebrates, but has lost precision with the accumulation of anecdotal reports not addressing key elements of the behaviour as first defined. We review the literature and propose three criteria that are sufficient and necessary to define the behaviour: (1 individuals form a circle; (2 defensive attributes of the individuals are positioned on the periphery of the circle, and as a result, the periphery of the circle uniformly contains either heads or abdomens; (3 animals preemptively adopt the circle as a resting formation, meaning it is not necessary to observe predation. When these considerations are taken into account, cycloalexy appears less common in nature than the literature suggests. We argue that unequivocal cases of cycloalexy have been found only in sawflies (Tenthredinoidea: Pergidae, Argidae, leaf beetles (Chrysolemidae: Galerucinae, Cassidinae, Chrysomelinae, Criocerinae, weevils (Curculionidae: Phelypera distigma, and midges (Diptera: Ceratopogonidae, Forcipomyia. Reports of cycloalexy in caterpillars (Saturniidae: Hemileucinae: Lonomia, Papilionidae require further documentation. We report one new case of cycloalexy in thrips (Thysanoptera and question reports of cycloalexic behaviour in other taxa.

  10. Entomopathogenic fungus as a biological control for an important vector of livestock disease: the Culicoides biting midge.

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    Minshad Ali Ansari

    Full Text Available BACKGROUND: The recent outbreak of bluetongue virus in northern Europe has led to an urgent need to identify control measures for the Culicoides (Diptera: Ceratopogonidae biting midges that transmit it. Following successful use of the entomopathogenic fungus Metarhizium anisopliae against larval stages of biting midge Culicoides nubeculosus Meigen, we investigated the efficacy of this strain and other fungi (Beauveria bassiana, Isaria fumosorosea and Lecanicillium longisporum as biocontrol agents against adult C. nubeculosus in laboratory and greenhouse studies. METHODOLOGY/FINDINGS: Exposure of midges to 'dry' conidia of all fungal isolates caused significant reductions in survival compared to untreated controls. Metarhizium anisopliae strain V275 was the most virulent, causing a significantly decrease in midge survival compared to all other fungal strains tested. The LT(50 value for strain V275 was 1.42 days compared to 2.21-3.22 days for the other isolates. The virulence of this strain was then further evaluated by exposing C. nubeculosus to varying doses (10(8-10(11 conidia m(-2 using different substrates (horse manure, damp peat, leaf litter as a resting site. All exposed adults were found to be infected with the strain V275 four days after exposure. A further study exposed C. nubeculosus adults to 'dry' conidia and 'wet' conidia (conidia suspended in 0.03% aq. Tween 80 of strain V275 applied to damp peat and leaf litter in cages within a greenhouse. 'Dry' conidia were more effective than 'wet' conidia, causing 100% mortality after 5 days. CONCLUSION/SIGNIFICANCE: This is the first study to demonstrate that entomopathogenic fungi are potential biocontrol agents against adult Culicoides, through the application of 'dry' conidia on surfaces (e.g., manure, leaf litter, livestock where the midges tend to rest. Subsequent conidial transmission between males and females may cause an increased level of fungi-induced mortality in midges thus

  11. Environmental drivers of Culicoides phenology: how important is species-specific variation when determining disease policy?

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    Kate R Searle

    Full Text Available Since 2006, arboviruses transmitted by Culicoides biting midges (Diptera: Ceratopogonidae have caused significant disruption to ruminant production in northern Europe. The most serious incursions involved strains of bluetongue virus (BTV, which cause bluetongue (BT disease. To control spread of BTV, movement of susceptible livestock is restricted with economic and animal welfare impacts. The timing of BTV transmission in temperate regions is partly determined by the seasonal presence of adult Culicoides females. Legislative measures therefore allow for the relaxation of ruminant movement restrictions during winter, when nightly light-suction trap catches of Culicoides fall below a threshold (the 'seasonally vector free period': SVFP. We analysed five years of time-series surveillance data from light-suction trapping in the UK to investigate whether significant inter-specific and yearly variation in adult phenology exists, and whether the SVFP is predictable from environmental factors. Because female vector Culicoides are not easily morphologically separated, inter-specific comparisons in phenology were drawn from male populations. We demonstrate significant inter-specific differences in Culicoides adult phenology with the season of Culicoides scoticus approximately eight weeks shorter than Culicoides obsoletus. Species-specific differences in the length of the SVFP were related to host density and local variation in landscape habitat. When the Avaritia Culicoides females were modelled as a group (as utilised in the SFVP, we were unable to detect links between environmental drivers and phenological metrics. We conclude that the current treatment of Avaritia Culicoides as a single group inhibits understanding of environmentally-driven spatial variation in species phenology and hinders the development of models for predicting the SVFP from environmental factors. Culicoides surveillance methods should be adapted to focus on concentrated assessments

  12. Algal-mediated ecosystem exchanges in the Eel River drainage network: towards photogrammetric mapping of color to function

    Science.gov (United States)

    Power, M. E.; Welter, J.; Furey, P.; Lowe, R.; Finlay, J. C.; Hondzo, M.; Limm, M.; Bode, C.; Dietrich, W. E.

    2009-12-01

    Seasonal algal proliferations in river networks are typically short-lived (weeks-months) but spatially extensive. They mediate important ecological and biogeochemical exchanges within and between ecosystems. We are investigating correspondence of assemblage color with ecosystem function in the nitrogen-limited Eel River of northern California. During summer base flow following winter floods, Eel algal assemblages are dominated by the green macroalga Cladophora glomerata. New growths are green, but blooms turn yellow as Cladophora filaments are colonized by epiphytic diatoms (Cocconeis spp.). Later, proliferations turn rust colored as epiphytic assemblages became dominated by Epithemia spp., diatoms that contain nitrogen-fixing cyanobacterial endosymbionts. Epithemia-encrusted Cladophora occurs at and downstream of reaches draining > 100 km2 (where summer inundated average channel widths > 25 m), coinciding with a threshold increase in concentration of total dissolved nitrogen. Areal nitrogen fixation rates are 14x higher in rusty algal proliferations than in green, and 3-4x higher than in yellow Cladophora mats. Corresponding increases in insect emergence suggest that nitrogen fixed by cyanobacterial endosymbionts is highly edible. Rates of biomass emergence from rusty Cladophora mats are 12-17 times greater than from green mats, and 8-10 times greater from rusty than from yellow Cladophora mats, because larger taxa emerge from rusty mats (Chironominae versus Ceratopogonidae in yellow mats). Photogrammetric detection of spatial coverage and color changes in algal proliferations may help us track nitrogen fluxes they mediate (riverine loading from the atmosphere via fixation, river to the watershed return via insect emergence) that link riverine to aerial, watershed, and potentially nearshore marine ecosystems at reach to basin scales.

  13. Hyaluronidase of bloodsucking insects and its enhancing effect on leishmania infection in mice.

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    Vera Volfova

    hyaluronidase activity, namely blackflies (Simuliidae, biting midges (Ceratopogonidae and horse flies (Tabanidae.

  14. Culicoides species composition and environmental factors influencing African horse sickness distribution at three sites in Namibia.

    Science.gov (United States)

    Liebenberg, Danica; Piketh, Stuart; Labuschagne, Karien; Venter, Gert; Greyling, Telane; Mienie, Charlotte; de Waal, Tania; van Hamburg, Huib

    2016-11-01

    African horse sickness (AHS) is one of the most lethal infectious, non-contagious, vector-borne disease of equids. The causative agent, African horse sickness virus (AHSV) is transmitted via Culicoides midges (Diptera: Ceratopogonidae). AHS is endemic to Namibia but detailed studies of Culicoides communities and influencing environmental parameters are limited. This study aims to determine the Culicoides species composition at three different sites and to assess environmental parameters influencing the geographical distribution of AHS in Namibia. Weekly collections of Culicoides were made during the AHS peak season from January to May for 2013 and 2014 using the Onderstepoort 220V UV-light trap. Out of 397 collections made, 124 collections (3287 Culicoides) were analysed for AHSV presence with RT-qPCR. A total of 295 collections were analysed for total Culicoides (all collected Culicoides individuals) and in 75% of these collections the Culicoides were identified to species level. C. imicola was the dominant species with proportional representation of 29.9%. C. subschultzei, C. exspectator and C. ravus each contribute more than 10% to the species composition. The lowest number of Culicoides was collected at Aus 9980, a total of 21819 at Windhoek and the highest number at Okahandja 47343. AHSV was present at all three sites during 2013 but only in Windhoek and Okahandja during 2014. Multivariate analyses of data from the two year survey indicate the environmental parameters in order of importance for the distribution of AHS in Namibia as precipitation>temperature>clay>relative humidity>NDVI. The implication of these findings is that any precipitation event increases Culicoides numbers significantly. Together with these results the high number of species found of which little is known regarding their vector competence, add to the complexity of the distribution of AHS in Namibia. PMID:27491343

  15. [Agricultural land use impacts on aquatic macroinvertebrates in small streams from La Vieja river (Valle del Cauca, Colombia].

    Science.gov (United States)

    Giraldo, Lina Paola; Chará, Julián; Zúñiga, Maria del Carmen; Chará-Serna, Ana Marcela; Pedraza, Gloria

    2014-04-01

    The expansion of the agricultural frontier in Colombia has exerted significant pressure on its aquatic ecosystems during the last few decades. In order to determine the impacts of different agricultural land uses on the biotic and abiotic characteristics of first and second order streams of La Vieja river watershed, we evaluated 21 streams located between 1,060 and 1,534 m asl in the municipalities of Alcalá, Ulloa, and Cartago (Valle del Cauca, Colombia). Seven streams were protected by native vegetation buffers, eight had influence of coffee and plantain crops, and six were influenced by cattle ranching. Habitat conditions, channel dimensions, water quality, and aquatic macroinvertebrates were studied in each stream. Streams draining cattle ranching areas had significantly higher dissolved solids, higher phosphorus, higher alkalinity, higher conductivity, and lower dissolved oxygen than those covered by cropland and forests. Coarse substrates and diversity of flow regimes were significantly higher in cropland and protected streams when compared to streams affected by cattle ranching, whereas the percent of silt and slow currents was significantly higher in the latter. A total of 26,777 macroinvertebrates belonging to 17 orders, 72 families and 95 genera were collected. The most abundant groups were Diptera 62.8%, (Chironomidae 49.6%, Ceratopogonidae 6.7%), Mollusca 18.8% (Hydrobiidae 7.2%, Sphaeriidae 9.6%) and Trichoptera 5.7% (Hydropsychidae 3.7%). The Ephemeroptera, Trichoptera, and Plecoptera orders, known for their low tolerance to habitat perturbation, had high abundance in cropland and forested streams, whereas Diptera and Mollusca were more abundant in those impacted by cattle ranching. Results indicate that streams draining forests and croplands have better physical and biological conditions than those draining pastures, and highlight the need to implement protective measures to restore the latter. PMID:25189079

  16. First phase of macroinvertebrate repopulation of Lake Orta (Buccione Basin after liming

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    Angela BOGGERO

    2001-02-01

    Full Text Available The research described here was performed during 1993 and 1994 in the context of studies on the recovery of Lake Orta from acidification after the liming conducted from May 1989 to June 1990. The causes of the pollution were due to the oxidation of ammonium sulphate discharged from a factory producing rayon by a cupro-ammoniacal method and to the loads of heavy metals (Cu, Cr, Ni, Zn derived from plating factories. One year after liming, an increase in pH and a marked decrease in metal concentrations were observed, and as a consequence some qualitative and quantitative changes were observed in 1992-93 in the planctonic communities and in the littoral macrobenthic fauna. This paper presents qualitative and quantitative data on macrobenthos collected along a transect between the littoral and the maximal depth in the Buccione Basin, which was directly affected by the effluents of the rayon factory. The data revealed some quantitative repopulation of this community, especially along the littoral. Qualitatively, however, the macrobenthos was still quite poor, consisting essentially of Oligochaeta and Diptera Chironomidae. The former were rare and mainly represented by Naididae between 15 and 22 m, while the Tubificidae were well represented in the littoral and the profundal zones. As regards the Chironomidae, the genera Procladius and Chironomus gr. thummi extended as far as the maximum depth, while others, such as Ablabesmyia, Parakiefferiella bathophila and Dicrotendipes gr. nervosus were mostly distributed in the littoral layers. Among the other taxonomic groups only the Trichoptera and the Diptera Ceratopogonidae were quantitatively significant. However, many systematic entities typical of the less polluted Italian subalpine lakes are still absent. In fact, the colonisation of Lake Orta by macrobenthic organisms is still influenced by the increased concentrations of heavy metals in the sediment precipitated from the water column. The research

  17. Culicoides species abundance and potential over-wintering of African horse sickness virus in the Onderstepoort area, Gauteng, South Africa

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    Gert J. Venter

    2014-02-01

    Full Text Available In South Africa, outbreaks of African horse sickness (AHS occur in summer; no cases are reported in winter, from July to September. The AHS virus (AHSV is transmitted almost exclusively by Culicoides midges (Diptera: Ceratopogonidae, of which Culicoides imicola is considered to be the most important vector. The over-wintering mechanism of AHSV is unknown. In this study, more than 500 000 Culicoides midges belonging to at least 26 species were collected in 88 light traps at weekly intervals between July 2010 and September 2011 near horses in the Onderstepoort area of South Africa. The dominant species was C. imicola. Despite relatively low temperatures and frost, at least 17 species, including C. imicola, were collected throughout winter (June–August. Although the mean number of midges per night fell from > 50 000 (March to < 100 (July and August, no midge-free periods were found. This study, using virus isolation on cell cultures and a reverse transcriptase polymerase chain reaction (RT-PCR assay, confirmed low infection prevalence in field midges and that the detection of virus correlated to high numbers. Although no virus was detected during this winter period, continuous adult activity indicated that transmission can potentially occur. The absence of AHSV in the midges during winter can be ascribed to the relatively low numbers collected coupled to low infection prevalence, low virus replication rates and low virus titres in the potentially infected midges. Cases of AHS in susceptible animals are likely to start as soon as Culicoides populations reach a critical level.

  18. Temporal and altitudinal variations in benthic macroinvertebrate assemblages in an Andean river basin of Argentina

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    Erica E. Scheibler

    2014-02-01

    Full Text Available Environmental variables and benthic macroinvertebrate assemblages were spatially and seasonally examined over two consecutive years (2000-2002 along a glacier and snowmelt river in the central-west of Argentina where lies the highest peak in America, Mount Aconcagua (6956 m elevation. The goal was to assess seasonal and altitudinal variability in benthic community structure and to define whether physical-chemical variables affect distribution of aquatic insects. The Mendoza river basin was characterised by high variability in flow and transparency, high conductivity, hard calcium sulphate water, neutral and alkaline pH, and dominant substrate composed of small blocks, cobbles, pebbles, and sand-silt. Richness of invertebrates was low, with the lowest taxonomic richness being recorded at the mouth. The dominant group with highest taxonomic richness was Diptera, although caddisflies, mayflies, beetles, and stoneflies were present. Seasonal and spatial variations in biotic and abiotic variables were detected. Maximal densities and taxonomic richness were recorded in autumn and winter. From Modified Morisita’s Cluster analysis it was found that the system is divided into two groupings of sites related to each other by faunal composition. INDVAL revealed species turnover along the altitudinal gradient of some taxa: Andesiops, Massartellopsis, Edwarsina, Chelifera, and Ceratopogonidae had preference for the headwaters (2835-2425 m elevation, Smicridea murina and Baetodes for the lower section (1413-1085 m elevation, and Austrelmis for the middle and lower sections. The middle section (1846-1727 m elevation was a transition area where taxa from the headwaters and the lower section coexisted. Generalised Linear Models evidenced that altitude was the major factor determining macroinvertebrate assemblages along the large arid Mendoza River and that the physical-chemical variables that most influenced variation in community structure were: transparency

  19. Culicoides species abundance and potential over-wintering of African horse sickness virus in the Onderstepoort area, Gauteng, South Africa.

    Science.gov (United States)

    Venter, Gert J; Labuschagne, Karien; Majatladi, Daphney; Boikanyo, Solomon N B; Lourens, Carina; Ebersohn, Karen; Venter, Estelle H

    2014-01-01

    In South Africa, outbreaks of African horse sickness (AHS) occur in summer; no cases are reported in winter, from July to September. The AHS virus (AHSV) is transmitted almost exclusively by Culicoides midges (Diptera: Ceratopogonidae), of which Culicoides imicola is considered to be the most important vector. The over-wintering mechanism of AHSV is unknown. In this study, more than 500 000 Culicoides midges belonging to at least 26 species were collected in 88 light traps at weekly intervals between July 2010 and September 2011 near horses in the Onderstepoort area of South Africa. The dominant species was C. imicola. Despite relatively low temperatures and frost, at least 17 species, including C. imicola, were collected throughout winter (June-August). Although the mean number of midges per night fell from > 50 000 (March) to < 100 (July and August), no midge-free periods were found. This study, using virus isolation on cell cultures and a reverse transcriptase polymerase chain reaction (RT-PCR) assay, confirmed low infection prevalence in field midges and that the detection of virus correlated to high numbers. Although no virus was detected during this winter period, continuous adult activity indicated that transmission can potentially occur. The absence of AHSV in the midges during winter can be ascribed to the relatively low numbers collected coupled to low infection prevalence, low virus replication rates and low virus titres in the potentially infected midges. Cases of AHS in susceptible animals are likely to start as soon as Culicoides populations reach a critical level. PMID:25686125

  20. Aquatic insects of New York salt marsh associated with mosquito larval habitat and their potential utility as bioindicators.

    Science.gov (United States)

    Rochlin, Ilia; Dempsey, Mary E; Iwanejko, Tom; Ninivaggi, Dominick V

    2011-01-01

    The aquatic insect fauna of salt marshes is poorly characterized, with the possible exception of biting Diptera. Aquatic insects play a vital role in salt marsh ecology, and have great potential importance as biological indicators for assessing marsh health. In addition, they may be impacted by measures to control mosquitoes such as changes to the marsh habitat, altered hydrology, or the application of pesticides. Given these concerns, the goals of this study were to conduct the first taxonomic survey of salt marsh aquatic insects on Long Island, New York, USA and to evaluate their utility for non-target pesticide impacts and environmental biomonitoring. A total of 18 species from 11 families and five orders were collected repeatedly during the five month study period. Diptera was the most diverse order with nine species from four families, followed by Coleoptera with four species from two families, Heteroptera with three species from three families, then Odonata and the hexapod Collembola with one species each. Water boatmen, Trichocorixa verticalis Fieber (Heteroptera: Corixidae) and a shore fly, Ephydra subopaca Loew (Diptera: Ephydridae), were the two most commonly encountered species. An additional six species; Anurida maritima Guérin-Méneville (Collembola: Neanuridae), Mesovelia mulsanti White (Heteroptera: Mesovelidae), Enochrus hamiltoni Horn (Coleoptera: Hydrophilidae), Tropisternus quadristriatus Horn (Coleoptera: Hydrophilidae), Dasyhelea pseudocincta Waugh and Wirth (Diptera: Ceratopogonidae), and Brachydeutera argentata Walker (Diptera: Ephydridae), were found regularly. Together with the less common Erythrodiplax berenice Drury (Odonata: Libellulidae), these nine species were identified as the most suitable candidates for pesticide and environmental impact monitoring due to abundance, position in the food chain, and extended seasonal occurrence. This study represents a first step towards developing an insect-based index of biological integrity for

  1. Testing of UK Populations of Culex pipiens L. for Schmallenberg Virus Vector Competence and Their Colonization.

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    Robyn Manley

    Full Text Available Schmallenberg virus (SBV, an arboviral pathogen of ruminants, emerged in northern Europe during 2011 and has subsequently spread across a vast geographic area. While Culicoides biting midges (Diptera: Ceratopogonidae have been identified as a biological transmission agent of SBV, the role of mosquitoes (Diptera: Culicidae as potential vectors has not been defined beyond small-scale field collections in affected areas. Culex pipiens L. are one of the most widespread mosquitoes in northern Europe; they are present on farms across the region and have previously been implicated as vectors of several other arboviruses. We assessed the ability of three colony lines of Cx. pipiens, originating from geographically diverse field populations, to become fully infected by SBV using semi-quantitative real-time RT-PCR (sqPCR.Two colony lines of Cx. pipiens were created in the UK ('Brookwood' and 'Caldbeck' from field collections of larvae and pupae and characterised using genetic markers. A third strain of Cx. pipiens from CVI Wageningen, The Netherlands, was also screened during experiments. Intrathoracic inoculation of the Brookwood line resulted in infections after 14 days that were characterised by high levels of RNA throughout individuals, but which demonstrated indirect evidence of salivary gland barriers. Feeding of 322 individuals across the three colony lines on a membrane based infection system resulted in no evidence of full dissemination of SBV, although infections did occur in a small proportion of Cx. pipiens from each line.This study established two novel lines of Cx. pipiens mosquitoes of UK origin in the laboratory and subsequently tested their competence for SBV. Schmallenberg virus replication and dissemination was restricted, demonstrating that Cx. pipiens is unlikely to be an epidemiologically important vector of the virus in northern Europe.

  2. Trypanosomes and haemosporidia in the buzzard (Buteo buteo) and sparrowhawk (Accipiter nisus): factors affecting the prevalence of parasites.

    Science.gov (United States)

    Svobodová, Milena; Weidinger, Karel; Peške, Lubomír; Volf, Petr; Votýpka, Jan; Voříšek, Petr

    2015-02-01

    The prevalences of heteroxenous parasites are influenced by the interplay of three main actors: hosts, vectors, and the parasites themselves. We studied blood protists in the nesting populations of raptors in two different areas of the Czech Republic. Altogether, 788 nestlings and 258 adult Eurasian sparrowhawks (Accipiter nisus) and 321 nestlings and 86 adult common buzzards (Buteo buteo) were screened for parasites by the microscopic examination of blood smears and by cultivation. We examined the role of shared vectors and parasite phylogenetic relationships on the occurrence of parasites. In different years and hosts, trypanosome prevalence ranged between 1.9 and 87.2 %, that of Leucocytozoon between 1.9 and 100 %, and Haemoproteus between 0 and 72.7 %. Coinfections with Leucocytozoon and Trypanosoma, phylogenetically distant parasites but both transmitted by blackflies (Simuliidae), were more frequent than coinfections with Leucocytozoon and Haemoproteus, phylogenetically closely related parasites transmitted by different vectors (blackflies and biting midges (Ceratopogonidae), respectively). For example, 16.6 % buzzard nestlings were coinfected with Trypanosoma and Leucocytozoon, while only 4.8 % with Leucocytozoon and Haemoproteus and 0.3 % with Trypanosoma and Haemoproteus. Nestlings in the same nest tended to have the same infection status. Furthermore, prevalence increased with the age of nestlings and with Julian date, while brood size had only a weak negative/positive effect on prevalence at the individual/brood level. Prevalences in a particular avian host species also varied between study sites and years. All these factors should thus be considered while comparing prevalences from different studies, the impact of vectors being the most important. We conclude that phylogenetically unrelated parasites that share the same vectors tend to have similar distributions within the host populations of two different raptor species.

  3. Culicoides vector species on three South American camelid farms seropositive for bluetongue virus serotype 8 in Germany 2008/2009.

    Science.gov (United States)

    Schulz, Claudia; Ziller, Mario; Kampen, Helge; Gauly, Matthias; Beer, Martin; Grevelding, Christoph G; Hoffmann, Bernd; Bauer, Christian; Werner, Doreen

    2015-12-15

    Palearctic species of Culicoides (Diptera, Ceratopogonidae), in particular of the Obsoletus and Pulicaris complexes, were identified as putative vectors of bluetongue virus serotype 8 (BTV-8) on ruminant farms during the epizootic in Germany from 2006 to 2009. BTV may cause severe morbidity and mortality in ruminants and sporadically in South American camelids (SAC). However, the fauna of Culicoides spp. on SAC farms has not been investigated. Therefore, the ceratopogonid fauna was monitored on three farms with BTV-seropositive SAC in Germany. Black-light traps were set up on pastures and in stables from summer 2008 to autumn 2009. Additionally, ceratopogonids were caught in emergence traps mounted on llama dung and dung-free pasture from spring to autumn 2009. After morphological identification, selected Culicoides samples were analysed for BTV-RNA by real-time RT-PCR. The effects of the variables 'location', 'temperature' and 'humidity' on the number of Culicoides caught in black-light traps were modelled using multivariable Poisson regression. In total, 26 species of Culicoides and six other genera of biting midges were identified. The most abundant Culicoides spp. collected both outdoors and indoors with black-light traps belonged to the Obsoletus (77.4%) and Pulicaris (16.0%) complexes. The number of Culicoides peaked in summer, while no biting midges were caught during the winter months. Daily collections of Culicoides were mainly influenced by the location and depended on the interaction of temperature and humidity. In the emergence traps, species of the Obsoletus complex predominated the collections. In summary, the absence of BTV-RNA in any of the analysed Culicoides midges and in the BTV-seropositive SAC on the three farms together with the differences in the pathogenesis of BTV-8 in SAC compared to ruminants suggests a negligible role of SAC in the spread of the virus. Although SAC farms may provide similar suitable habitats for putative Culicoides

  4. 饶河口岸吸血蚊蠓本底调查%Baseline investigation on bloodsucking mosquitoes and midges at Raohe port in China

    Institute of Scientific and Technical Information of China (English)

    李永久; 于巍; 张阳; 刘国平

    2011-01-01

    目的 调查黑龙江省饶河口岸吸血蚊、蠓的种类分布和种群组成.方法 采用人帐诱、诱虫灯诱和网捕法采集吸血蚊、蠓,收集相关文献.结果 2008-2009年在饶河口岸采获吸血蚊、蠓90214只,计6属63种,其中蚊类4属28种;蠓类2属35种,哈尔巴岭库蠓为饶河口岸首次记录的蠓种;优势蚊种为刺扰伊蚊(84.94%);吸血蠓的主要种类是兴安库蠓(37.19%)、不显库蠓(20.03%)和刺螫库蠓(20.04%).不同生境、场所的主要蚊、蠓种类组成有所不同.结论 为黑龙江省饶河口岸吸血蚊、蠓本底、虫媒病和防制研究提供了科学依据.%Objective To investigate the species distribution and population composition of bloodsucking mosquitoes and midges at Raohe port in Heilongjiang province. Methods Bed curtains, light traps and insect nets were used to collect mosquitoes and bloodsucking midges. Results From 2008 to 2009, 90 214 mosquitoes and bloodsucking midges were captured in the region, which belonged to 63 species of 6 genera, 2 families, including 28 species, 4 genera of Culicoidae and 35 species, 2 genera of Ceratopogonidae. Culicoides haerbalingeruis were recorded for the first time at Raohe port. The predominant species of mosquitoes was Aedes vaxans (84.94%). The predominant species of bloodsucking midges were Culicoides sinanoennis (37.19%), C. Obsoletus (20.03%) and C. Punctatus (20.04%). The population composition of mosquitoes and bloodsucking midges varied in different habitats. Conclusion This investigation provided baseline data on bloodsucking mosquitoes and midges for formulation of the prevention and control strategies.

  5. A survey of Culicoides developmental sites on a farm in northern Spain, with a brief review of immature habitats of European species.

    Science.gov (United States)

    González, Mikel; López, Sergio; Mullens, Bradley A; Baldet, Thierry; Goldarazena, Arturo

    2013-01-16

    Culicoides species (Diptera: Ceratopogonidae) belonging to the Obsoletus and Pulicaris groups are considered to be the main vectors of bluetongue virus (BTV) in non Mediterranean Europe. Selected terrestrial microhabitats (n=17) on a farm in northern Spain were sampled repeatedly over a year-long period and characterized for use by Culicoides species for immature development. Concurrent use of CDC light traps showed the presence of 37 species and 66,575 specimens of adult Culicoides. A total of 28 species and 11,396 individuals emerged from laboratory-maintained soil samples. Culicoides obsoletus and Culicoides scoticus (pooled as Obsoletus complex) were particularly abundant (comprising 58.6% and 74.5% of the total collections in light traps and emergence traps respectively). Potential key vectors of animal viruses (such as BTV) were found in two main terrestrial types of microhabitats. In the case of C. obsoletus, different types of manure (old and composted manure, manure mixed with organic matter, and fresh manure) produced most of the specimens. In contrast, larvae of C. scoticus and Culicoides lupicaris were associated with soil substantially comprised of rotting leaf litter that included the parasitic plant Lathraea clandestina. Several species, Culicoides festivipennis, Culicoides punctatus and Culicoides brunnicans, were very common in mud at pond margins. Indeed, pond microhabitats and runoff below barn rooflines supported the greatest species richness. In the pond habitat, 49.4% of Culicoides specimens emerged from mud at the water edge, as opposed to 50 cm above (32.4%) and 1 meter above waterline (18%). Similar species richness, but statistically significant differences in abundance, were observed among the four pond microhabitats. Overall, the majority of the specimens were found in the upper layer (0-3 cm), except in manure, where they preferred deeper layers (>6 cm). Maximum peaks of abundance occurred in both light traps and soil samples in summer

  6. Arboviruses pathogenic for domestic and wild animals.

    Science.gov (United States)

    Hubálek, Zdenek; Rudolf, Ivo; Nowotny, Norbert

    2014-01-01

    The objective of this chapter is to provide an updated and concise systematic review on taxonomy, history, arthropod vectors, vertebrate hosts, animal disease, and geographic distribution of all arboviruses known to date to cause disease in homeotherm (endotherm) vertebrates, except those affecting exclusively man. Fifty arboviruses pathogenic for animals have been documented worldwide, belonging to seven families: Togaviridae (mosquito-borne Eastern, Western, and Venezuelan equine encephalilitis viruses; Sindbis, Middelburg, Getah, and Semliki Forest viruses), Flaviviridae (mosquito-borne yellow fever, Japanese encephalitis, Murray Valley encephalitis, West Nile, Usutu, Israel turkey meningoencephalitis, Tembusu and Wesselsbron viruses; tick-borne encephalitis, louping ill, Omsk hemorrhagic fever, Kyasanur Forest disease, and Tyuleniy viruses), Bunyaviridae (tick-borne Nairobi sheep disease, Soldado, and Bhanja viruses; mosquito-borne Rift Valley fever, La Crosse, Snowshoe hare, and Cache Valley viruses; biting midges-borne Main Drain, Akabane, Aino, Shuni, and Schmallenberg viruses), Reoviridae (biting midges-borne African horse sickness, Kasba, bluetongue, epizootic hemorrhagic disease of deer, Ibaraki, equine encephalosis, Peruvian horse sickness, and Yunnan viruses), Rhabdoviridae (sandfly/mosquito-borne bovine ephemeral fever, vesicular stomatitis-Indiana, vesicular stomatitis-New Jersey, vesicular stomatitis-Alagoas, and Coccal viruses), Orthomyxoviridae (tick-borne Thogoto virus), and Asfarviridae (tick-borne African swine fever virus). They are transmitted to animals by five groups of hematophagous arthropods of the subphyllum Chelicerata (order Acarina, families Ixodidae and Argasidae-ticks) or members of the class Insecta: mosquitoes (family Culicidae); biting midges (family Ceratopogonidae); sandflies (subfamily Phlebotominae); and cimicid bugs (family Cimicidae). Arboviral diseases in endotherm animals may therefore be classified as: tick

  7. Health and sanitary status in 1970 of Tubu nomads dwelling in Northeastern Niger

    Institute of Scientific and Technical Information of China (English)

    Jean-Franois Magnaval; Christian Oosterbosch; Michel Mandl; MABN group

    2014-01-01

    -fluke eggs were found. The stool samples were mixed with a preservative MIF solution and then stored to be examined later in Toulouse. Three subjects (2%) passedE. histolytica/E. dispar cysts in stools, 16 (10.6%) were parasitized with Giardia sp. and 4 (2.65%) were parasitized with Hymelepis nana. Two specimens of scorpions captured in the camp were subsequently identified as belonging to the harmful genus Androctonus or Leiurus. An investigation into the freshwater fauna was conducted in the marshy ponds surrounding the ghost city of Djado, and no intermediate snail hosts for schistosomiasis haematobium were found. Numerous nymphs of Ceratopogonidae, which are possible vectors for arboviruses of veterinarian importance, were collected, as were larvae and nymphs from two anopheline species, Anopheles hispaniola and An. multicolor, which are not efficient vectors for malaria. Conclusions: Infection-related blindness and trachoma, along with acute pulmonary infections and probably tuberculosis were the major health burden in this tribe. The harsh dry and hot climate may explain the low prevalence of soil-transmitted protozoan diseases or helminthiases.

  8. The test report of monitoring equine infectious anemia and eliminate the blood-sucking insects in Baise%百色市马传染性贫血监测及扑灭吸血昆虫试验报告

    Institute of Scientific and Technical Information of China (English)

    黄百花; 覃勇; 蒙振亩; 陆春礼; 赵聪; 卢少坤

    2015-01-01

    In order to eliminate the epidemic of Equine infectious anemia and strengthen the results of prevention and control in baise.From 1990 to 2014, successively using AGID test, single spots of ELISA and immune strip rapid test monitoring the 9844 copies of Equine infectious anemia antibodies, the results are all negative;To find out the media of the disease as Ceratopogonidae , Mosquito that blood-sucking insects with virus, from 2011 to 2014, RT-PCR method were used to detect the 370 samples of blood sucking insects in the boundary village with Yunnan province,all the results are negative;To carried out using three drugs of efficient cypermethrin, bromine cypermethrin and double sulphur phosphorous pesticide particles for blood sucking insects killing test, the results show that the efficient cypermethrin disinsection effect is best, the double sulfur phosphorus pesticide particles drug delivery using more convenient and simple, vigorously promote the use after test, effectively control the spread of the Equine infectious anemia by blood-sucking insects in Baise .To through a series of monitoring and control measures for Equine infectious anemia in 25 years, the test results are negative, have reached the target of eliminating Equine infectious anemia.%为了消灭百色市马传贫疫情并巩固防治成果,1990年至2014年先后使用马传贫琼脂凝胶免疫扩散试验配合斑点单抗ELISA和抗体快速检测卡等对采自辖区9844份马属动物血清进行检测,结果全部为阴性;为了探明该病的传播媒介蠓、蚊、刺蝇及虻等吸血昆虫带毒情况,2011年至2014年,使用RT-PCR法对采自与疫区云南省交界村屯370份吸血昆虫样品进行检测,结果全部为阴性;开展使用高效氯氰菊酯、溴氯氰菊酯和双硫磷杀虫颗粒等三种药物对吸血昆虫灭杀效果试验,结果显示高效氯氰菊酯的灭虫效果最好,双硫磷杀虫颗粒投药使用比较方便简单,试验后大力推广使用,有效

  9. Investigation on diptera insect of bloodsucking in Heixiazi Island%中国黑瞎子岛吸血双翅目昆虫调查研究

    Institute of Scientific and Technical Information of China (English)

    富英群; 侯咏; 王晓杰; 李明; 王红霞; 周春燕; 刘国平; 于永军

    2013-01-01

    Objective To investigate the community composition and numbers variation of predominan community of mosquitoes, midges and horseflies in Heixiazi Island of Fuyuan county in Heilongjiang province. Methods Human-baited net traps, light traps and insect nets were used to capture mosquitoes, midges and horseflies. Results A total of 37 272 diptera insects of bloodsucking were captured and identified, which belonged to family Culicidae 4 genera 10 species, family Ceratopogonidae 2 genera 5 species.and family Tabanidae 5 genera 15 species. The predominan community were Aedes vaxans (94.68%), Culicoides punctatus (84.69%), Atylotus miser (29.84%) and Tabanus griseinus (23.90%), respectively. The predominant community composition of mosquitoes varied in different months. Numbers variation of 4 species mosquitoes within 24 h showed that active houres were Aedes vexans within 24 h, Aedes sticticus at 3:00AM to 21:00PM, Culex modestus at 6:00AM to 20:00PM, and Anopheles sinensis at 19:00PM to 3:00AM; Two peaks were appeared for Aedes vexans at 3:00AM and 20:00PM, only one peak for Culex modestus at 17: 00PM, Aedes sticticus at 18:00PM, and Anopheles sinensis at 21:00PM within 24 h. Active hours of 6 species horseflies were during 4:00AM~ 19:00PM in a day. Only one peak showed at 14:00PM for Tabanus griseinus, Atylotus miser and Atylotus rusticus; at 15:00PM for Atylotus horvathi; at 16:00PM for Tabanus pleskei; and at 18:00PM for Tabanus amaenus. Conclusion The investigation provides the referencing basis for the study on community composition, numbers variation of community, and control of diptera insect of bloodsucking in Heixiazi Island of Heilongjiang province.%目的 调查黑龙江省抚远县黑瞎子岛吸血双翅目昆虫蚊、蠓、虻的种群构成,主要种群的昼夜数量动态.方法 采用人帐诱、诱虫灯诱和网捕法采集蚊、蠓、虻.结果 在黑瞎子岛采获蚊、蠓、虻37272只,经分类鉴定,其中蚊科4属10