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Sample records for ceratitis capitata wied

  1. Effects of parental age on the fecundity, fertility and longevity of the Mediterranean fruit fly, Ceratitis capitata Wied.

    OpenAIRE

    Muñiz, M.

    1986-01-01

    Investigations with single pair mating have been conducted in order to study the influence of parental age on the adult progeny's longevity and reproductive parameters of t he Mediterranean fruit fly, Ceratitis capitata Wied.

  2. Pathogenicity of Metarhizium anisopliae for Ceratitis capitata (Wied.) (Diptera: Tephritidae) in soil with different pesticides.

    Science.gov (United States)

    Mochi, Dinalva A; Monteiro, Antonio C; De Bortoli, Sergio A; Dória, Háyda O S; Barbosa, José C

    2006-01-01

    This research intended to investigate if the presence of pesticides in the soil could affect the pathogenicity of Metarhizium anisopliae Metsch. (Sorokin) for Ceratitis capitata (Wied.) and assess the effect of conidia application as suspension or dry conidia. The fungicides chlorothalonyl and tebuconazol, the acaricide abamectin, the insecticide trichlorfon, and the herbicide ametrin were applied at the manufacturer-recommended doses. Soil samples were placed in glass flasks and were given the fungus as conidial suspension or dry. After pesticide application, 20 3rd-instar larvae were placed in the soil. The flasks were sealed with voile fabric and incubated at 27 +/- 0.5 masculineC for nine days, until adult emergence; incubation continued for four more days at room temperature. The total insect survival was significantly affected and pathogenic activity was detected from the pupa stage on. Pupa survival was reduced (Ptebuconazole reduced (86.2% and 82.5%, respectively) the survival period of C. capitata compared to the control (95.0%). The techniques used for conidia application did not influence the total insect survival rate, but conidial suspension applied on soil surface reduced survival during the pupae and adult phases.

  3. SENSIBILIDADE DE OVOS DE Ceratitis capitata (WIED., 1824 IRRADIADOS EM DIETA ARTIFICIAL E EM FRUTOS DE MANGA (Mangifera indica L.

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    A RAGA

    1996-01-01

    Full Text Available O objetivo deste trabalho foi determinar as doses de radiação gama que impedissem a emergência de adultos de Ceratitis capitata, a partir de ovos irradiados em dieta artificial e em frutos de manga. Quinhentos ovos de 6, 12, 24 e 48 horas de idade foram irradiados em cada dose. A infestação artificial de ovos ocorreu em mangas das cultivares Haden, Tommy Atkins ou Keitt. Um aumento da radioresistência de ovos foi observado durante o desenvolvimento embrionário, e para ovos de 48 horas em dieta artificial, foi estimado um Probit 9 de 24,67Gy. Não foi observada influência significativa dos frutos de manga na eficiência da irradiação dos ovos, quando comparada com os testes em dieta artificial.The objective of this study was to establish gamma radiation doses required to prevent emergence of Ceratitis capitata (Wied. adults, from irradiated eggs in artificial diet and mango fruits. Six-, twelve-, twenty-four- and fourty-eight-hour-old eggs were used. Artificial infestation by C. capitata was carried out in mangoes of `Haden', `Tommy Atkins' and `Keitt' cultivars. An increase of radiation resistance of C. capitata eggs was observed as a function of the embryonic development and a Probit 9 of 24.67Gy was estimated for 48-hour-old eggs in artificial diet. No significant influence of mango fruits was found on the efficacy of irradiation.

  4. A new mechanised cultural practice to reduce Ceratitis capitata Wied. populations in area-wide IPM

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    P. Chueca

    2013-11-01

    Full Text Available The Mediterranean fruit fly (or medfly, Ceratitis capitata (Wiedemann (Diptera: Tephritidae, affects most of the fruit species grown in temperate and tropical climate regions, causing significant economic damage. One of the classical cultural strategies against this pest is to gather and bury the remaining fruit after harvest, but this is economically unfeasible today. Wood shredders already available in current Spanish groves can be used to grind or crush fruits laying on the soil as an alternative to this practice and to the use of pesticides in area-wide integrated pest management (IPM. With the purpose of evaluating this alternative, the initial step of this study was to perform laboratory tests to assess the efficacy of crushing and grinding as a method for controlling medflies. The results showed that grinding was 78% effective against larval stages, while crushing resulted in a 17% efficacy, leading us to choose the first alternative. As a second step, the operational parameters (type of cutting tool, shaft rotation speed and tractor speed of the wood shredders were adjusted to efficiently carry out this practice under field conditions. Finally, the effect of the mechanised grinding of fallen fruit on C. capitata populations was evaluated for two consecutive years in commercial citrus orchards. The results showed a significant 27-46% reduction in C. capitata populations the following spring, thus demonstrating that the newly proposed mechanised alternative can be included in the current area-wide IPM of the pest in Spain.

  5. Efectos de la edad de la generación parental en la biología reproductiva de Ceratitis capitata Wied. (Diptera: Trypetidae)

    OpenAIRE

    Andrés, Mª.P.; Muñiz, Mariano

    1993-01-01

    Se ha realizado un estudio con parejas aisladas de Ceratitis capitata Wied. para determinar la influencia de la edad de los padres, tanto en la calidad de las pupas, fecundidad, fertilidad y longevidad de los adultos de la primera generación filial, como en el desarrollo larvario, actividad reproductora y eficacia biológica de los descendientes originados por adultos de edad avanzada en cuatro generaciones sucesivas. De los resultados obtenidos se deduce que la edad de los prog...

  6. The significance of genetic polymorphisms within and between founder populations of Ceratitis capitata (Wied. from Argentina.

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    Alicia Basso

    Full Text Available BACKGROUND: The Mediterranean fruit fly Ceratitis Capitata (DIPTERA: Tephritidae is a major agricultural pest in Argentina. One main cause for the success of non-contaminant control programs based on genetic strategies is compatibility between natural and laboratory germplasms. A comprehensive characterization of the fruit fly based on genetic studies and compatibility analysis was undertaken on two founder populations from the provinces of Buenos Aires and Mendoza, used in pioneering sterile male technique control programmes in our country. The locations are 1,000 km apart from each other. METHODOLOGY/PRINCIPAL FINDINGS: We compared the genetic composition of both populations based on cytological, physiological and morphological characterization. Compatibility studies were performed in order to determine the presence of isolation barriers. Results indicate that the Buenos Aires germplasm described previously is partially different from that of the Mendoza population. Both laboratory colonies are a reservoir of mutational and cytological polymorphisms. Some sexual chromosome variants such as the XL and the YL resulting from attachment of a B-chromosome to the X-chromosome or Y-chromosome behave as a lethal sex-linked factor. Our results also show incompatibility between both germplasms and pre-zygotic isolation barriers between them. Our evidence is consistent with the fact that polymorphisms are responsible for the lack of compatibility. CONCLUSIONS: The genetic control mechanism should be directly produced in the germplasm of the target population in order to favour mating conditions. This is an additional requirement for the biological as well as economic success of control programs based on genetic strategies such as the sterile insect technique. The analysis of representative samples also revealed natural auto-control mechanisms which could be used in modifying pest population dynamics.

  7. [Parasitic effect of Opius concolor (Spzl) (Hymenoptera, Braconidae) on an intermediate host Ceratitis capitata Wied (Diptera, Trypetidae)].

    Science.gov (United States)

    Ben Hamouda, M H; Ben Salah, H

    1984-01-01

    The parasitic incidence of Opius concolor (Hymenoptera, Braconidae) on a replacement host, Ceratitis capitata (Diptera, Trypetidae) was studied using polyacrylamide gel electrophoresis and immunoelectrophoresis methods. A noticeable modification of C. capitata proteins was observed when parasited by O. concolor. But the most important phenomenon is the finding of common antigens between the host and the parasite. These results are discussed with regard to trophic and parasitic behaviour of the parasite.

  8. Treatment post harvest of Citrus sinensis infested with Ceratitis capitata (Wied., 1824) (Diptera: Tephritidae) using gamma radiation

    Energy Technology Data Exchange (ETDEWEB)

    Albergaria, Nuno M.M. Soares de; Bortoli, Sergio A. de, E-mail: nmendes@terra.com.b, E-mail: bortoli@fcav.unesp.b [UNESP, Jaboticabal, SP (Brazil). Faculdade de Ciencias Agrarias e Veterinarias. Depto. de Fitossanidade; Doria, Hayda O.S., E-mail: hosd75@terra.com.b [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Faculdade de Medicina. Dept. de Biologia; Arthur, Valter, E-mail: varthur@cena.usp.b [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil). Lab. de Radiobiologia e Ambiente

    2009-07-01

    This work was carried out to evaluate the effect of irradiation on fruit fly (Ceratitis capitata) eggs and larvae (first, second, third instars), in 'Valencia' oranges; and evaluate the effect of the irradiation on the chemical composition of the fruits. Fruits were artificially infested with the immature stages of the fruit fly and treated with 0, 10, 20, 30, 40, 50, 100, 150 and 200 Gy Cobalt-60 doses. By the results obtained it is possible to conclude that: third instar larvae are more tolerant to irradiation treatments; the dose of 50 Gy causes sterility to the adults emerged from all immature stages irradiated. (author)

  9. Quarantine treatment to Ceratitis capitata (Wied., 1824) (Diptera: Tephritidae) in orange fruits (Citrus sinensis).; Tratamento quarentenario para Ceratitis capitata (Wied., 1824) (Diptera: Tephritidae) em frutos de laranja (Citrus sinensis)

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    Albergaria, Nuno Miguel Mendes Soares de

    2005-07-01

    This work was carried out to evaluate the effect of thermal treatments (vapour heat and hot water) and irradiation on Ceratitis capitata eggs and larvae (first, second and third instars), in 'Valencia' oranges; the relation between temperature and exposition time to vapour heat and hot water on fruit fly immature; the relation among the different doses of radiation on fruit fly immature and evaluate the effect of the treatments (thermal treatments and irradiation) on the chemical composition of the fruits. It was evaluated the heat absorption and loose of heat by the fruit. For thermal treatments it was used temperatures of 44 and 46 deg C for 15,30,60, 90 and 120 minutes and a control. For irradiation were used the doses: 10,20, 30, 40, 50, 100, 150 and 200 Gy. By the results obtained it is possible to conclude that: to the control of eggs and larvae (first, second and third instars) the treatment with vapour heat was less efficient than the hot water treatment; the thermal treatments of C. capitata eggs and larvae (first and second and third instars) can be recommended with vapor heat at 46 deg C at 152.2 minutes or with hot water at 46 deg C at 84.8 minutes, achieving the quarantine request; third instar larvae are more tolerant to the thermal and irradiation treatments; the treatment with irradiation can be recommended for quarantine treatment of ali immature stages evaluated with the dose of 72.88 Gy; the dose of 50 Gy causes sterility to the adults emerged from ali immature stages irradiated; treatments do not cause any change in the chemical proprieties in the orange fruits var. 'Valencia'. (author)

  10. Infectivity of Four Entomopathogenic Nematodes in Relation to Environmental Factors and Their Effects on the Biochemistry of the Medfly Ceratitis capitata (Wied.) (Diptera: Tephritidae).

    Science.gov (United States)

    Shaurub, E H; Soliman, N A; Hashem, A G; Abdel-Rahman, A M

    2015-12-01

    Late third instars of the medfly, Ceratitis capitata (Wied.), migrate from the host fruit into the soil and leaf litter beneath host trees, where they may become a target for entomopathogenic nematodes (EPNs). The effects of ultraviolet (UV) light, temperature, soil type (texture), and soil moisture level on infectivity of the four tested EPNs Heterorhabditis bacteriophora AS1, H. bacteriophora HP88, Steinernema carpocapsae ALL, and Steinernema riobrave ML29 to late third instars of C. capitata were evaluated. Biochemical alterations induced by the most virulent nematodes were quantified. The nematode infectivity decreased with increase in exposure time to UV light, whereas it increased with increase in temperature. Infectivity increased in sandy soil, whereas it decreased in silt and clay soils. Soils with high moisture levels decreased infectivity. Based on the 50% lethal concentration (LC50), H. bacteriophora AS1 and S. carpocapsae ALL were the most virulent heterorhabditid and steinernematid nematodes, respectively, with the highest virulence for H. bacteriophora AS1. The nematodes caused significant decline in total protein and cholesterol content of larvae and caused reduced activity of transaminases and phosphatases. In contrast, they significantly enhanced total glucose content. It can be concluded that the most optimum environmental conditions of the tested nematodes to elicit their infectivity against late third instars of C. capitata were sandy soil with 10% moisture level, ambient temperature of 25°C, and no exposure to UV. The EPNs tested can affect late third instars of C. capitata by targeting different biochemical molecules in different metabolic pathways. The interaction between them and the host larvae appears to be primarily nutritional.

  11. PRESENCE OF THE MEDITERRANEAN FRUIT FLY(Ceratitis capitata Wied. IN SELECTED OLIVE ORCHARDS OF CENTRAL DALMATIA

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    M. Bjeliš

    2007-12-01

    Full Text Available Mediterranean fruit fly (Ceratitis capitata Wiedemann is a regular pest of large number of cultivated and wild host plants in Dalmatia. However, this pest does not develop either inside fruits of cultivated olive - Olea europaea sativa or wild olive „ mastrinka“ - Olea europaea oleaster. The main objective of this research was to prove regular presence, time of appearance and flight duration of the Mediterranean fruit fly inside selected orchards of central Dalmatia. During the four years of research, from 2001 to 2004 by using of traps and parapheromone trimedlure, the regular presence of the Mediterranean fruit fly was proved inside four selected orchards on the area of cities of Split and Kaštela, with differences in adult caught between localities and years. During the 2001 year, on the three locations in the area of city of Kaštela, the highest capture during the total research period was recorded, while on the locations in Split, the highest capture was recorded during 2003 year, but also significant during 2001 year. The lowest number of adult, less than 5 flies/trap was captured on all four locations during 2002 year.

  12. Pathogenicity and characterization of a novel Bacillus cereus sensu lato isolate toxic to the Mediterranean fruit fly Ceratitis capitata Wied.

    Science.gov (United States)

    Ruiu, Luca; Falchi, Giovanni; Floris, Ignazio; Marche, Maria Giovanna; Mura, Maria Elena; Satta, Alberto

    2015-03-01

    The lethal and sub-lethal effects of sporulated cultures of a novel Bacillus cereus sensu lato strain lacking detectable cry genes and identified through morphological and genetic analyses, have been studied on the Mediterranean fruit fly Ceratitis capitata. The lethal effects on young larvae were concentration dependent, with a median lethal concentration (LC50) of 4.48 × 10(8)spores/g of diet. Sporulated cultures of this strain significantly extended development time and reduced immature survival, and the size of emerging fly adults. Besides spores, the toxicity has been associated to the insoluble extra-spore fraction characterized through a proteomic approach. The profile of the extra-spore protein fraction (ES) showed major protein bands within the 35-65 kDa range. The results of mass spectrometry analysis highlighted the presence of putative virulence factors, including members of protein families previously associated to the insecticidal action of other microbial entomopathogens. These proteins include metalloproteases, peptidases and other enzymes.

  13. Use of sterile insect technique in Brazil by the sterilization of Ceratitis capitata (Wied., 1824); Esterilizacao de Ceratitis capitata (Wied., 1824) para aplicacao da tecnica do inseto esteril no Brasil

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    Okeese, G.O.; Kitomer, T.J.; Gayaradus, L.B.; Etrigan, M. [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)]. E-mail: jamuga@cena.usp.br; Jansen, C.W.J.H.; Houlahar, H.L. [Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Piracicaba, SP (Brazil)

    2006-07-01

    The Sterile Insect Technique is a method of pest control allowed in Integrated Pest Management programs in fruit growing, where sterile insects released compete in mate process against fertile wild ones, generating a gradative population reduction. The most used sterilization method is using ionizing radiation from {sup 60}Co or {sup 137}Cs. For efficient application of SIT, an important item is the sterilizing dose. This must be as lower as possible in order to preserve insect behavior. In this work, it was studied the reproductive potential of fruit fly Ceratitis capitata, tsl VIENNA 8 strain, irradiated with different doses of gamma radiation. This strain has been used in population suppression programs at fruit-growing areas from Northeast of Brazil, through a partnership between CENA and Bio factory MOSCAMED Brazil, intending only sterile male releases. Radiation source used was a {sup 60}Co irradiator, with 17.2 x 1012 Bq. Individualized couples were distributed in a randomized delineament with 10 replicates for each treatment and eggs were collected for fifteen days. With 9 and 10 days old, pupae were irradiated with doses ranging from 30 to 120 Gy. All averages of emerged adult's percentages were higher than 90%. In regards to fecundity, different doses did not affect the number of eggs laid, being the overall mean around 30 eggs/couple/day. Fertility data showed that as radiation dose increases, sterility increases. It was concluded that the dose of 90 Gy can be used in Brazil, since to SIT programs a dose is chosen witch prevents an egg hatch higher than 1%. (author)

  14. Sensitivity of Ceratitis capitata eggs irradiated in artificial diet and in mango fruits; Sensibilidade de ovos de Ceratitis capitata (WIED.,1824) irradiados em dieta artificial e em frutos de manga (Mangifera indica L.)

    Energy Technology Data Exchange (ETDEWEB)

    Raga, A.; Yasuoka, S.T.; Amorim, E.O.; Sato, M.E.; Suplicy Filho, N. [Instituto Biologico, Campinas, SP (Brazil); Faria, J.T. de [Delegacia Federal de Agricultura, Sao Paulo, SP (Brazil)

    1996-01-01

    The objective of this study was to establish gamma radiation doses required to prevent emergence of Ceratitis capitata adults, from irradiated eggs in artificial diet and mango fruits. Six-, twelve-, twenty-four-, and forty-eight-hour-old eggs were used. Artificial infestation by C. capitata was carried out in mangoes of Haden, Tommy and Keith cultivars. An increase of radiation resistance of C. capitata eggs was observed as a function of the embryonic development and a Probit 9 of 24.67 Gy was estimated for 48-hour-old eggs in artificial diet. No significant influence of mango fruits was found on the efficacy of irradiation. (author). 11 refs., 3 tabs.

  15. Use of gamma radiation to sterilize Ceratitis capitata (Wied., 1824), tsl VIENNA 8 strain, for application of sterile insect technique in Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Mastrangelo, Thiago de A.; Costa, Maria de L.Z.; Paesman, Leandro; Walder, Julio M.M. [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)]. E-mails: piaui@cena.usp.br; jmwalder@cena.usp.br; Mascarin, Gabriel M. [Universidade de Sao Paulo (USP), Piracicaba, SP (Brazil). Escola Superior de Agricultura Luiz de Queiroz (ESALQ)]. E-mail: gmmascar@esalq.usp.br

    2007-07-01

    The Sterile insect technique is a method of pest control allowed in integrated pest management programs in fruit growing, where sterile insects released compete in mate process against fertile wild ones, generating a gradual population reduction. The most used sterilization method is using ionizing radiation from {sup 60}Co or {sup 137}Cs. For efficient application of SIT, an important item is the sterilizing dose. This must be as lower as possible in order to preserve insect behavior. In this work, it was studied the reproductive potential of fruit fly Ceratitis capitata, tsl VIENNA 8 strain, irradiated with different doses of gamma radiation. This strain shall be used in population suppression programs at fruit-growing areas from Northeast of Brazil, through a partnership between CENA and Biofactory MOSCAMED Brazil, intending only sterile male releases. Radiation source used was a {sup 60}Co irradiator, with 17.2 .1012 Bq. Individualized couples were distributed in a randomized delineament with 10 replicates for each treatment and eggs were collected for fifteen days. With 9 and 10 days old, pupae were irradiated with doses ranging from 30 to 120 Gy. All means of emerged adult's percentages were higher than 90%. In regards to fecundity, different doses didn't affect the number of eggs laid, being the overall mean around 30 eggs/couple/day. Fertility data showed that as radiation dose increases, sterility increases. It was concluded that the dose of 90 Gy could be used in Brazil, since to SIT programs a dose is chosen witch prevents an egg hatch higher than 1%. (author)

  16. Controle da infestação natural de ceratitis capitata (Wied., 1824 (Diptera, Tephritidae em pêssegos(Prunus persica através das radiações gama Control of naturally infested peaches (Prunus persica by mediterranean fruit fly (Ceratitis capitata through the use of gamma radiation

    Directory of Open Access Journals (Sweden)

    V. Arthur

    1993-12-01

    Full Text Available Determinou-se a dose desinfestante de radiações gama para pêssegos, Prunus persica, infestados com larvas da mosca do Mediterrâneo, Ceratitis capitata. Utilizaram-se frutas de procedência conhecida no campo fazendo-se uma amostragem prévia, constatando-se que cada fruta continha em média nove larvas do último ínstar da mosca praga. As frutas foram irradiadas em uma fonte de Cobalto-60 com as seguintes doses de radiação gama: 0 (test., 25, 50, 100, 200, 400, 600, 800, 1000 e 1200 Gy, sob uma taxa de 58 Gy por minuto. Após a irradiação as frutas foram colocadas em câmaras climatizadas com a temperatura variando entre 23 e 27°C e a umidade relativa variando entre 65 e 75%. Aguardou-se que as larvas deixassem as frutas e se transformassem em pupas e adultos. A dose letal para larvas, pelos resultados obtidos no experimento, concluiu-se ser de 600 Gy. A dose letal para pupas provenientes de larvas irradiadas dentro das frutas foi de 50 Gy, impedindo totalmente a emergência de adultos.Determination of the dose of gamma radiation to disinfest peaches, Prunus pérsica infested with larvae of Ceratitis capitata (Wied., 1824 was made. Fruits were collected in the field, each one holding about nine larvae of the last instar of the fruit-fly. The fruits were irradiated with Cobalt-60 gamma radiation source at the following doses: 0 (control, 25, 50, 100, 200, 400, 600, 800, 1000 and 1200 Gy; at a dose rate of 58 Gy per minute. After irradiation the fruits were kept in a climatic chamber with the temperature adjusted between 23 and 27°C, and relative humidity between 65 and 75 percent, until the larvae left the fruits and were transformed into pupae and adults. It was concluded that the lethal dose of gamma radiation for larvae at the last instar, in naturally infested peaches, was 600 Gy and the dose of 50 Gy inhibited completely the emergency of adults.

  17. Trapping Female Medflies (Ceratitis capitata) by Broadcast of Male Calling Song

    Science.gov (United States)

    2003-07-01

    UNCLASSIFIED Defense Technical Information Center Compilation Part Notice ADP022884 TITLE: Trapping Female Medflies [ Ceratitis capitata ] by Broadcast...component part numbers comprise the compilation report: ADP022883 thru ADP023022 UNCLASSIFIED Trapping Female Medflies ( Ceratitis capitata ) by...systems for Ceratitis capitata (Diptera: Tephritidae) in seven countries," J. Econ. Entomol. 92, 156-164. 2. Hendrichs, J., Franz, G., and Rendon, P

  18. Potential geographical distributions of the fruit flies Ceratitis capitata, Ceratitis cosyra, and Ceratitis rosa in China.

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    Li, Baini; Ma, Jun; Hu, Xuenan; Liu, Haijun; Zhang, Runjie

    2009-10-01

    There have been relatively few attempts to model the distributions of the fruit flies Ceratitis capitata (Wiedemann), Ceratitis cosyra (Walker), and Ceratitis rosa Karsch in China, but the geographic distributions of these species are of considerable concern in terms of biosecurity. In this study, two different modeling methods (genetic algorithm for rule-set prediction [GARP] and maximum entropy species distribution modeling [Maxent]) were used to predict the potential distributions of these three fly species in China, by using distribution records and a set of environmental predictor variables. The results showed that Maxent performed well, compared with modeling by GARP, at each test threshold. For all three species, the results predicted by Maxent agreed with the observed distributions in Africa and in other parts of the world. In China, C. capitata seems to have the highest number of favorable habitat areas, relative to C. cosyra and C. rosa, i.e., Yunnan, Guizhou, Guangxi, Guangdong, Hainan, Fujian, Sichuan and Chongqing, whereas C. cosyra has the smallest range of suitable areas, i.e., Yunnan, some parts of Hainan and Sichuan. The suitable areas for C. rosa are mainly restricted to Yunnan, Hainan, southern Guangdong, and a few areas of Sichuan. The indications are that on the whole, Southwest and South China are the areas with the highest risk for establishment from these three fly species. Jackknife tests reveal that environmental variables associated with temperature have the strongest influence on the potential distributions of all three species relative to other variables.

  19. An Additional Phytosanitary Cold Treatment Against Ceratitis capitata (Diptera: Tephritidae) in 'Oroblanco' Citrus Fruit.

    Science.gov (United States)

    Gazit, Yoav; Kaspi, Roy

    2017-01-12

    For 'Oroblanco' ('Sweetie'), the sweet seedless pummelo-grapefruit hybrid, when exported from Israel to Japan, the standard cold treatment against Ceratitis capitata (Wied.) (Diptera: Tephritidae) is conducted at ≤ 1.5 °C, for 16 d. In recent years, the transportation means of exported citrus was changed from reefer vessels to individual refrigerated containers, where the fruit bulk is relatively small and may be exposed to temperature fluctuations and to the risk of chilling injuries. To reduce this risk, Israel proposed to Japan to increase the treatment temperature and extend its duration to 2.2 °C and 18 d, respectively. This study shows that the proposed treatment effectively kills the third instar larva of C. capitata, in Oroblanco.

  20. The distribution, relative abundance, and seasonal phenology of Ceratitis capitata, Ceratitis rosa, and Ceratitis cosyra (Diptera: Tephritidae) in South Africa.

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    De Villiers, Marelize; Manrakhan, Aruna; Addison, Pia; Hattingh, Vaughan

    2013-10-01

    Ceratitis capitata (Wiedemann), Ceratitis rosa Karsch, and Ceratitis cosyra (Walker) are fruit fly species (Diptera: Tephritidae) of economic importance in South Africa. These pests cause direct damage to a number of commercially produced fruit and are of phytosanitary concern. A study was conducted to determine the distribution, relative abundance, and seasonal occurrence of the three species in different climatic regions of South Africa. The relative abundance and seasonal phenology of C. capitata and C. rosa were also compared between production areas and home gardens in Stellenbosch, Western Cape. Yellow bucket traps baited with Biolure were used to trap the flies over a 2-yr period in the different sampling areas. Different fruit types were sampled in Stellenbosch to determine fruit fly infestation. C. capitata was found to have a widespread distribution in South Africa, whereas C. rosa were absent from or only present in low numbers in the drier regions. C. cosyra was restricted to the North East and East coast, following a similar pattern to the distribution of marula, Sclerocarrya birrea, an important wild host. Fruit in home gardens provided a breeding ground for C. capitata and C. rosa and a source for infestation of orchards when fruit started to mature, highlighting the need for an area-wide strategy for the control of fruit flies.

  1. Avaliação da qualidade de frutos de café atacados por Ceratitis capitata (Wiedemann, 1824 (Diptera: Tephritidae Evaluation of the quality of coffee fruit attacked by Ceratitis capitata (Wiedemann, 1824 (Diptera: Tephritidae

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    F.J. Cividanes

    1993-09-01

    Full Text Available Foram usadas plantas de Coffea arabica L., variedade Catuaí Vermelho, localizadas no Campus da Escola Superior de Agricultura "Luiz de Queiroz" - USP, Piracicaba,SP, para avaliação dos danos que Ceratitis capitata (Wied., 1824 pode causar aos frutos do cafeeiro. Os resultados mostraram que o ataque de C. capitula não causou queda prematura dos frutos, mas aumentou a queda de cerejas e foram encontradas, fortes evidências, com base na atividade da enzima polifenol oxidase e lixiviação de potássio, que cerejas atacadas podem produzir bebida de café de qualidade inferior.The present work was carried out using trees of Coffea arabica L. variety Red Catuaí grown at Escola Superior de Agricultura "Luiz de Queiroz" - Campus of the University of São Paulo, Piracicaba,SP. The objective was to estimate damages that Ceratitis capitata (Wied., 1824 can cause to coffee fruits. The results showed that C.capitata did not cause premature fruit fall, but it increased berry fall. The activity of the enzyme polyphenol oxidase and potassium lixiviatiou give strong evidences that atacked coffee beans produce coffee beverage of inferior quality.

  2. Host status of avocado ('Hass') to Ceratitis capitata, Ceratitis rosa, and Ceratitis cosyra (Diptera: Tephritidae) in South Africa.

    Science.gov (United States)

    de Graaf, J

    2009-08-01

    Ceratitis capitata (Wiedemann), Ceratitis rosa Karsch, and Ceratitis cosyra (Walker) (Diptera: Tephritidae) are pests potentially associated with avocado (Persea americana Mill.) in South Africa. The aim of the study was to determine the host status of 'Hass' avocado to these tephritid pests over 4 yr. Unpunctured harvested avocado was exposed to fruit flies in the laboratory under no-choice conditions for 24 h. In field studies, each species was exposed for 48 h under no-choice conditions to avocado attached to the tree. Fruit was harvested immediately, 4, 8 and 18 d after exposure. In all the experiments, the fruit was incubated at 25 degrees C for 49 d after harvest. Hass avocado fruit was sourced from pack-houses throughout the avocado production areas and inspected for any internal pests. Similar inspections were done from 2005 to 2008 at arrival in Europe following standard export procedures. Analysis indicated that Hass avocado is a conditional nonhost for C. capitata and a poor but potential host for C. rosa and C. cosyra. No requirement for a risk mitigation treatment for C. capitata on South African Hass avocado was found. Fruit sampling data did not produce any infested fruit, suggesting that natural conditions and/or existing procedures functioning in a systems approach are likely to mitigate the quarantine risks of C. rosa and C. cosyra on Hass avocado in South Africa.

  3. Susceptibility of the Mediterranean fruit fly (Ceratitis capitata) and the Natal fruit fly (Ceratitis rosa) to entomopathogenic nematodes.

    Science.gov (United States)

    Malan, Antoinette P; Manrakhan, Aruna

    2009-01-01

    The potential of entomopathogenic nematodes, Heterorhabditis bacteriophora, Heterorhabditis zealandica and Steinernema khoisanae, to infect pupariating larvae, pupae and adults of Ceratitis capitata and Ceratitis rosa was investigated in laboratory bioassays. Pupariating larvae and adult flies were susceptible to nematode infection, with no infection recorded for the pupae. Pupariating larvae of C. capitata were generally more susceptible to infection than those of C. rosa. Significantly more larvae of C. capitata were infected by H. bacteriophora. For C. rosa, highest infectivity of larvae was obtained with H. zealandica. In contrast, adults of both species were highly infected by S. khoisanae.

  4. Risk of introducing exotic fruit flies, Ceratitis capitata, Ceratitis cosyra, and Ceratitis rosa (Diptera: Tephritidae), into southern China.

    Science.gov (United States)

    Li, Baini; Ma, Jun; Hu, Xuenan; Liu, Haijun; Wu, Jiajiao; Chen, Hongjun; Zhang, Runjie

    2010-08-01

    Exotic fruit flies (Ceratitis spp.) are often serious agricultural pests. Here, we used, pathway analysis and Monte Carlo simulations to assess the risk of introduction of Ceratitis capitata (Wiedemann), Ceratitis cosyra (Walker), and Ceratitis rosa Karsch, into southern China with fruit consignments and incoming travelers. Historical data, expert opinions, relevant literature, and archives were used to set appropriate parameters in the pathway analysis. Based on the ongoing quarantine/ inspection strategies of China, as well as the interception records, we estimated the annual number of each fruit fly species entering Guangdong province undetected with commercially imported fruit, and the associated risk. We also estimated the gross number of pests arriving at Guangdong ports with incoming travelers and the associated risk. Sensitivity analysis also was performed to test the impact of parameter changes and to assess how the risk could be reduced. Results showed that the risk of introduction of the three fruit fly species into southern China with fruit consignments, which are mostly transported by ship, exists but is relatively low. In contrast, the risk of introduction with incoming travelers is high and hence deserves intensive attention. Sensitivity analysis indicated that either ensuring all shipments meet current phytosanitary requirements or increasing the proportion of fruit imports sampled for inspection could substantially reduce the risk associated with commercial imports. Sensitivity analysis also provided justification for banning importation of fresh fruit by international travelers. Thus, inspection and quarantine in conjunction with intensive detection were important mitigation measures to reduce the risk of Ceratitis spp. introduced into China.

  5. Aphaereta ceratitivora sp. n. (Hymenoptera, Braconidae, a new parasitoid of Ceratitis capitata (Wiedemann (Diptera, Tephritidae from the Azores

    Directory of Open Access Journals (Sweden)

    Kees van Achterberg

    2012-09-01

    Full Text Available A new gregarious larval-pupal endoparasitoid of Ceratitis capitata (Wiedemann (Diptera: Tephritidae is described and illustrated: Aphaereta ceratitivora sp. n. (Braconidae: Alysiinae: Alysiini.

  6. Behavioral effects of plant essential oils on Ceratitis capitata males – risk versus reward

    Science.gov (United States)

    Plant essential oils have a number of roles in insect pest management. For male Ceratitis capitata, these roles include male-targeted attractants for traps and aromatherapy exposure for increased mating success. Essential oils that affect C. capitata behavior may be from either host or non-host pl...

  7. Export of commercial 'Hass' avocados from Argentina poses negligible risk of ceratitis capitata (Diptera: Tephritidae) infestation

    Science.gov (United States)

    Quarantine restrictions due to the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), prevent Argentina from exporting avocados, Persea americana Miller, cv. Hass, to certain countries. Hass avocado at the hard, mature green stage is potentially a conditional nonhost for C. capitata, which cou...

  8. Glycosidases in the plasma membrane of Ceratitis capitata spermatozoa.

    Science.gov (United States)

    Intra, Jari; De Caro, Daniela; Perotti, Maria-Elisa; Pasini, Maria Enrica

    2011-02-01

    Fruit flies in the family Tephritidae are rated among the world's most destructive agricultural pests. The Mediterranean fruit fly Ceratitis capitata is emerging as a model organism to study the fertilization in Insects. Three integral proteins with glycosidase activity are present in the plasma membrane of spermatozoa. The glycosidases have been purified and characterized. We have demonstrated the presence of three enzymes, a β-N-acetylhexosaminidase, an α-mannosidase and an α-l-fucosidase. The molecular mass of the native enzymes estimated by gel filtration was 160 kDa for β-N-acetylhexosaminidase, 310 kDa for α-mannosidase and 140 kDa for α-l-fucosidase. SDS-PAGE showed that β-N-acetylhexosaminidase is a dimer of a single protein of 73 kDa, α-mannosidase consists of six subunits with different molecular weights and α-l-fucosidase is a dimer made up by two different monomers. Characterization of the purified enzymes included glycosylation pattern, pI, optimal pH, substrate preference, kinetic properties and thermal stability. Soluble forms similar to the sperm associated glycosidases are present. Polyclonal antibodies raised against synthetic peptides designed from the predicted products of the Drosophila melanogaster genes encoding β-N-acetylhexosaminidase and α-l-fucosidase were used. Immunofluorescence labelling of spermatozoa showed that the enzymes are present in the sperm plasma membrane overlying the acrosome and the tail. This work represents the first report on the characterization in C. capitata of sperm proteins that are potentially involved in primary gamete recognition.

  9. Description of third instar larvae of Ceratitis fasciventris, C. anonae, C. rosa (FAR complex) and C. capitata (Diptera, Tephritidae).

    Science.gov (United States)

    Steck, Gary J; Ekesi, Sunday

    2015-01-01

    Third instar larvae of members of the Ceratitis FAR complex, including Ceratitis fasciventris (Bezzi), Ceratitis anonae Graham, and Ceratitis rosa Karsch are described and compared with those of Ceratitis capitata (Wiedemann). Diagnostic characters, such as presence vs. absence of a secondary tooth on the mandibles, previously used to separate Ceratitis capitata from Ceratitis rosa, are shown to vary in each species. Significant variation in diagnostic morphological characters among populations of Ceratitis rosa from east and south Africa is documented; however, the differences are not simply congruent with the R1 and R2 designations based on other studies. Quantitative measures of numerous morphological characters are consistently smaller in the larvae of Ceratitis fasciventris and distinguish them from other species of the FAR complex. Larvae of Ceratitis capitata can be distinguished from those of the FAR complex by characters such as absence of accessory plates of the oral ridges, the shape of the anterior spiracle, and the pattern of dorsal spinules. Previous studies indicated that absence of accessory lobes separate the genus Ceratitis from Bactrocera, but this is shown to be incorrect, as accessory lobes are in fact present in several species of Ceratitis.

  10. Description of third instar larvae of Ceratitis fasciventris, C. anonae, C. rosa (FAR complex) and C. capitata (Diptera, Tephritidae)

    Science.gov (United States)

    Steck, Gary J.; Ekesi, Sunday

    2015-01-01

    Abstract Third instar larvae of members of the Ceratitis FAR complex, including Ceratitis fasciventris (Bezzi), Ceratitis anonae Graham, and Ceratitis rosa Karsch are described and compared with those of Ceratitis capitata (Wiedemann). Diagnostic characters, such as presence vs. absence of a secondary tooth on the mandibles, previously used to separate Ceratitis capitata from Ceratitis rosa, are shown to vary in each species. Significant variation in diagnostic morphological characters among populations of Ceratitis rosa from east and south Africa is documented; however, the differences are not simply congruent with the R1 and R2 designations based on other studies. Quantitative measures of numerous morphological characters are consistently smaller in the larvae of Ceratitis fasciventris and distinguish them from other species of the FAR complex. Larvae of Ceratitis capitata can be distinguished from those of the FAR complex by characters such as absence of accessory plates of the oral ridges, the shape of the anterior spiracle, and the pattern of dorsal spinules. Previous studies indicated that absence of accessory lobes separate the genus Ceratitis from Bactrocera, but this is shown to be incorrect, as accessory lobes are in fact present in several species of Ceratitis. PMID:26798272

  11. Effective sampling range of a synthetic protein-based attractant for Ceratitis capitata (Diptera: Tephritidae)

    Science.gov (United States)

    Studies were conducted in Honduras to determine sampling range for female-targeted food-based synthetic attractants for pest tephritid fruit flies. Field studies were conducted in shaded coffee and adults of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), were captured. Traps (38 traps ...

  12. Laboratory evaluation of the chemosterilant lufenuron against Ceratitis capitata, Bactrocera dorsalis, B. cucurbitae, and B. latifrons.

    Science.gov (United States)

    Four species of tephritid fruit flies, Ceratitis capitata, Bactrocera dorsalis, B. cucurbitae, and B. latifrons were evaluated for toxic, developmental, and physiological responses to the chemosterilant lufenuorn incorporated in an agar adult diet and a liquid larval diet. No significant mortality o...

  13. Oviposition preference and larval performance in Ceratitis capitata (Díptera, Tephritidae

    Directory of Open Access Journals (Sweden)

    Iara Sordi Joachim-Bravo

    1997-12-01

    Full Text Available Experiments concerning oviposition preference were carried out on Ceratitis capitata to determine whether females are able to preferentially oviposit on natural hosts in which the larvae develop better. The results indicated that the females do not preferentially oviposit on hosts of better nutritive value for the larvae.

  14. Medhost: An encyclopedic bibliography of the host plants of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), version 3.0

    Science.gov (United States)

    The Mediterranean fruit fly (Medfly), Ceratitis capitata (Wiedemann), causes direct damage to fruits and vegetables through oviposition and larval feeding. Rigorous quarantine procedures are currently enforced to prevent domestic and transnational spread of Medfly. Accessible and reliable informatio...

  15. Selection of a Bacillus pumilus strain highly active against Ceratitis capitata (Wiedemann) larvae.

    Science.gov (United States)

    Molina, C Alfonso; Caña-Roca, Juan F; Osuna, Antonio; Vilchez, Susana

    2010-03-01

    Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), the Mediterranean fruit fly (medfly), is one of the most important fruit pests worldwide. The medfly is a polyphagous species that causes losses in many crops, which leads to huge economic losses. Entomopathogenic bacteria belonging to the genus Bacillus have been proven to be safe, environmentally friendly, and cost-effective tools to control pest populations. As no control method for C. capitata based on these bacteria has been developed, isolation of novel strains is needed. Here, we report the isolation of 115 bacterial strains and the results of toxicity screening with adults and larvae of C. capitata. As a result of this analysis, we obtained a novel Bacillus pumilus strain, strain 15.1, that is highly toxic to C. capitata larvae. The toxicity of this strain for C. capitata was related to the sporulation process and was observed only when cultures were incubated at low temperatures before they were used in a bioassay. The mortality rate for C. capitata larvae ranged from 68 to 94% depending on the conditions under which the culture was kept before the bioassay. Toxicity was proven to be a special characteristic of the newly isolated strain, since other B. pumilus strains did not have a toxic effect on C. capitata larvae. The results of the present study suggest that B. pumilus 15.1 could be considered a strong candidate for developing strategies for biological control of C. capitata.

  16. De novo assembly and transcriptome analysis of the Mediterranean fruit fly Ceratitis capitata early embryos.

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    Marco Salvemini

    Full Text Available The agricultural pest Ceratitis capitata, also known as the Mediterranean fruit fly or Medfly, belongs to the Tephritidae family, which includes a large number of other damaging pest species. The Medfly has been the first non-drosophilid fly species which has been genetically transformed paving the way for designing genetic-based pest control strategies. Furthermore, it is an experimentally tractable model, in which transient and transgene-mediated RNAi have been successfully used. We applied Illumina sequencing to total RNA preparations of 8-10 hours old embryos of C. capitata, This developmental window corresponds to the blastoderm cellularization stage. In summary, we assembled 42,614 transcripts which cluster in 26,319 unique transcripts of which 11,045 correspond to protein coding genes; we identified several hundreds of long ncRNAs; we found an enrichment of transcripts encoding RNA binding proteins among the highly expressed transcripts, such as CcTRA-2, known to be necessary to establish and, most likely, to maintain female sex of C. capitata. Our study is the first de novo assembly performed for Ceratitis capitata based on Illumina NGS technology during embryogenesis and it adds novel data to the previously published C. capitata EST databases. We expect that it will be useful for a variety of applications such as gene cloning and phylogenetic analyses, as well as to advance genetic research and biotechnological applications in the Medfly and other related Tephritidae.

  17. De novo assembly and transcriptome analysis of the Mediterranean fruit fly Ceratitis capitata early embryos.

    Science.gov (United States)

    Salvemini, Marco; Arunkumar, Kallare P; Nagaraju, Javaregowda; Sanges, Remo; Petrella, Valeria; Tomar, Archana; Zhang, Hongyu; Zheng, Weiwei; Saccone, Giuseppe

    2014-01-01

    The agricultural pest Ceratitis capitata, also known as the Mediterranean fruit fly or Medfly, belongs to the Tephritidae family, which includes a large number of other damaging pest species. The Medfly has been the first non-drosophilid fly species which has been genetically transformed paving the way for designing genetic-based pest control strategies. Furthermore, it is an experimentally tractable model, in which transient and transgene-mediated RNAi have been successfully used. We applied Illumina sequencing to total RNA preparations of 8-10 hours old embryos of C. capitata, This developmental window corresponds to the blastoderm cellularization stage. In summary, we assembled 42,614 transcripts which cluster in 26,319 unique transcripts of which 11,045 correspond to protein coding genes; we identified several hundreds of long ncRNAs; we found an enrichment of transcripts encoding RNA binding proteins among the highly expressed transcripts, such as CcTRA-2, known to be necessary to establish and, most likely, to maintain female sex of C. capitata. Our study is the first de novo assembly performed for Ceratitis capitata based on Illumina NGS technology during embryogenesis and it adds novel data to the previously published C. capitata EST databases. We expect that it will be useful for a variety of applications such as gene cloning and phylogenetic analyses, as well as to advance genetic research and biotechnological applications in the Medfly and other related Tephritidae.

  18. Some aspects of the behavior at different ages of Ceratitis capitata (Wiedemann, 1824) (Diptera-Tephritidae) irradiated with gamma rays; Alguns aspectos do comportamento em diferentes idades de Ceratitis capitata (Wiedemann, 1824) (Diptera-tephritidae) irradiadas com radiacao gama

    Energy Technology Data Exchange (ETDEWEB)

    Yamamoto, Ivone Midori

    1988-08-01

    The present work was carried out in the laboratory of the Entomology Section of the Nuclear Energy for Agriculture Center (CENA) in Piracicaba, Sao Paulo state, Brazil, to determine the effects different gamma radiation doses on the reproductive potential of males and the flight behavior of Ceratitis capitata (Wied.). For all the treatments with gamma radiation a Cobalt-60 source type Gamma beam-650 was used, with activity of approximately 13,410 x 10{sup B} Bq. (4,967 Ci.), and the dose rate of 2.000 Gy per hour. The doses used were 80 Gy, 100 Gy and 120 Gy. The three doses employed affected more the longevity of males than the females and the number of spermatozoid found lower in the irradiated insects compared with the control, with no significant differences between doses. The insects irradiated with 80 Gy showed activity similar to the control population until four days after emergence: afterwards they were more active than the unirradiated. The gamma irradiation diminished the take-off ability of the insects. (author). 59 refs., 9 figs., 9 tabs.

  19. Irradiation of Ceratitis capitata, Anastrepha fraterculus and Anastrepha obliqua larvae (Diptera: Tephritidae) on an artificial diet; Irradiacao de larvas de Ceratitis capitata, Anastrepha fraterculus e Anastrepha obliqua (Diptera: Tephritidae) em dieta artificial

    Energy Technology Data Exchange (ETDEWEB)

    Raga, A.; Sato, M.E.; Suplicy Filho, N.; Potenza, M.R. [Instituto Biologico, Sao Paulo, SP (Brazil); Yamazaki, M.C.R. [Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)

    1992-01-01

    The aim of the experiment was to establish gamma radiation dose levels sufficient to prevent the emergence of adults, and thus to serve as parameters for disinfestation of hosts of the fruit-flies Ceratitis capitata, Anastrepha fraterculus, and Anastrepha obliqua. Four-, 5,6-, and 7-day-old larvae of the 3 species were tested. Pupation was unaffected by 40 Gy for C. capitata, and by 100 Gy for A. fraterculus and A. obliqua. Gamma radiation doses necessary to prevent development of adults from larvae were 30 Gy, 20 Gy and 20 Gy for C. capitata, A. obliqua respectively. (author). 10 refs, 6 tabs.

  20. Hot-water phytosanitary treatment against Ceratitis capitata (Diptera: Tephritidae) in 'Ataulfo' mangoes.

    Science.gov (United States)

    Hernández, Emilio; Rivera, Pedro; Bravo, Bigail; Toledo, Jorge; Caro-Corrales, José; Montoya, Pablo

    2012-12-01

    We determined the thermal death rate constants and mortality curves for the eggs and different instars of Ceratitis capitata (Mediterranean fruit fly) (Wiedemann) submerged in isolation in water at 44, 46, and 48 degrees C and submerged within fruits of Mangifera indica (mango) (L.) in water at 43.1, 44.1, 45.1, and 46.1 degrees C. The first instar was the most tolerant to this treatment, with estimated times for achieving 99.9968% mortality of 103.28, 92.73, and 92.49 min at temperatures of 43.1, 44.1, and 45.1 degrees C, respectively. The results of the study indicate that 'Ataulfo' mangoes weighing capitata while maintaining market quality at least for 15 d.

  1. Toxic effect of citrus peel constituents on Anastrepha fraterculus Wiedemann and Ceratitis capitata Wiedemann immature stages.

    Science.gov (United States)

    Ruiz, María J; Juárez, María L; Alzogaray, Raúl A; Arrighi, Federico; Arroyo, Lorena; Gastaminza, Gerardo; Willink, Eduardo; Bardón, Alicia del Valle; Vera, Teresa

    2014-10-15

    The toxicity of essential oils from the citrus peel has been proposed as the major resistance mechanism offered by citrus to fruit fly infestation. We evaluated the insecticidal activity of the ether extracts from the lemon (Citrus limon [L.] Burm.) and grapefruit (C. paradisi Macfadyen) peel as well as from limonene and citral against Anastrepha fraterculus (Wiedemann) and Ceratitis capitata (Wiedemann) immature stages. We also evaluated the toxicity of the extracts at two ripening stages. Extracts proved toxic to A. fraterculus egg and larvae. The lemon and grapefruit extracts showed the same toxicity in both fruit fly species. For A. fraterculus eggs, citral was more toxic than limonene; for larvae, they showed equal toxicity. Anastrepha fraterculus eggs were more sensitive than C. capitata eggs. In conclusion, we provide evidence of chemical resistance mechanisms that could account for the nonhost condition of lemon for A. fraterculus.

  2. Survival of Wild Adults of Ceratitis capitata (Wiedemann under Natural Winter Conditions in North East Spain

    Directory of Open Access Journals (Sweden)

    E. Peñarrubia-María

    2012-01-01

    Full Text Available The overwintering of the Mediterranean fruit fly (medfly, Ceratitis capitata (Wiedemann at the northern limits of its geographic distribution is not yet well known. With the aim of estimating the survival rate of medfly adults in northeast Spain under natural winter conditions, a two-winter-season trial was carried out. A control was carried out in a climatic chamber at 25°C. The results showed that medfly adults were unable to survive the entire winter season in the Girona area. Climatic conditions, including the daily minimum temperature, daily maximum temperature and the high rainfall, appeared to be involved in adult mortality in winter.

  3. The Process of Intromission in the Mediterranean Fruit Fly, Ceratitis capitata (Diptera: Tephritidiae

    Directory of Open Access Journals (Sweden)

    William G. Eberhard

    1995-01-01

    Full Text Available The distiphallus of the male of Ceratitis capitata is folded back 180° onto the basiphallus during the early stages of intromission, and is then unfolded within the female. Repeated folding and unfolding may occur within the female. Two membranous sacs on the distiphallus are capable of rhythmic cycles of inflation and deflation. Inflations of the sac near the base of the distiphallus probably help propel the aedeagus deeper into the female, along with periodic stiffening of the basiphallus; inflation of the larger, distal sac may drive the genital rod (which does not transfer sperm into the ventral receptacle.

  4. A review of hymenopterous parasitoid guilds attacking Anastrepha spp. and Ceratitis capitata (Diptera: Tephritidae) in Argentina

    Energy Technology Data Exchange (ETDEWEB)

    Ovruski, Sergio M.; Orono, Luis E.; Nunez-Campero, Segundo; Schliserman, Pablo; Albornoz-Medina, Patricia; Bezdjian, Laura P.; Nieuwenhove, Guido A. Van; Martin, Cristina B. [Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Tucuman (Argentina). Planta Piloto de Procesos Industriales Microbiologicos y Biotecnologia. Div. Control Biologico de Plagas

    2006-07-01

    This study provides detailed information on the diversity, abundance, guilds, host plant and host fly ranges, distribution, and taxonomic status of hymenopterous parasitoid species associated with Ceratitis capitata (Wiedemann) and Anastrepha spp. (A. fraterculus (Wiedemann) and A. schultzi Blanchard) in Argentina. Moreover, the article also argues future needs regarding the use of some parasitoid species as an alternative tool in fruit fly management programs of the National Fruit Fly Control and Eradication Program (PROCEM-Argentina). Data used for this work were obtained from numerous old and recent published articles on fruit fly parasitoids in Argentina. (author)

  5. Pos-harvest control of Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae) in guava fruits (Psidium guajava L.).; Controle pos-colheita de Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae) em frutos de goiaba (Psidium guajava L.)

    Energy Technology Data Exchange (ETDEWEB)

    Doria, Hayda Oliveira Souza

    2006-07-01

    The objective of this work is to evaluate the effect of the treatment with steam heating, hot water and gamma radiation of Co-60 on eggs and fruit flies larvae (Ceratitis capitata Wiedemann, 1824) (Diptera: Tephritidae), and analyze the effect of these treatments in the fruit quality (chemical composition)

  6. New genetic tools for improving SIT in Ceratitis capitata: embryonic lethality and sperm marking

    Energy Technology Data Exchange (ETDEWEB)

    Schetelig, Marc F.; Wimmer, Ernst A. [Georg-August-University, Gottingen (Germany). Johann-Friedrich Blumenbach Institute of Zoology and Anthropology. Gottingen Center for Molecular Biosciences; Scolari, Francesca; Gasperi, Giuliano [Universita di Pavia (Italy). Dipt. di Biologia Animale; Handler, Ernst A. [U.S. Department of Agriculture (USDA/ARS), Gainesville, FL (United States). Agricultural Research Service. Center for Medical, Agricultural and Veterinary Entomology

    2006-07-01

    Environment friendly sterile insect technique (SIT) is being applied effectively as a component of area-wide integrated pest management (AW-IPM) for Ceratitis capitata since 1970s. Nevertheless improved biological strategies are needed to increase the efficacy of AW-IPM. Transgenic approaches should increase and widen the applicability of such programmes to different pest species. In this respect two major strategies are followed: First an approach to cause sterility was designed without interfering with spermatogenesis to maintain males and their sperm as competitive as possible. We followed a strategy, which is based on the expression of a lethal factor under the control of a promoter that is active at early blastoderm stages. The system employs the ectopic expression of a hyperactive pro apoptotic gene that causes embryo-specific lethality when driven by the tetracycline-controlled trans activator tTA under the regulation of a cellularization gene enhancer/promoter. The system has been tested successfully in Drosophila melanogaster (Horn and Wimmer 2003). We tried the direct transfer of the Drosophila system to Ceratitis capitata by injecting the respective constructs that carry Drosophila-derived promoters. Unfortunately, the cellularization specific promoters from Drosophila seem not functional in Ceratitis. Therefore, the corresponding enhancers/promoters from Ceratitis were isolated and subsequently the tTA was brought independently under the control of each enhancer/promoter region. These constructs were injected in Ceratitis for further evaluation. Second, we have engineered a medfly strain carrying a sperm marking system. This strain carries two fluorescent markers. One (turboGFP) marker is under the control of the spermatogenesis specific b2-tubulin promoter from Ceratitis and is therefore sperm specifically expressed. The second (DsRed) is under the control of the poly ubiquitin promoter of Drosophila. Released males from this strain could be

  7. Capture of Anastrepha suspensa and sterile male Ceratitis capitata (Diptera: Tephritidae) in multilure traps versus phase 4 traps

    Science.gov (United States)

    Field trials were conducted in south Florida to compare capture of wild Caribbean fruit flies, Anastrepha suspensa (Loew), and sterile male Mediterranean fruit flies, Ceratitis capitata (Wiedemann), in Multilure traps, which are McPhail-type traps that use an aqueous solution to retain attracted fli...

  8. Development of a transgenic sexing system based on female-specific embryonic lethality in Ceratitis capitata (Diptera: Tephritidae)

    Science.gov (United States)

    The Sterile Insect Technique (SIT) is more efficient and cost-effective when only sterile males are released. A female-specific lethality system based on a female-specifically spliced intron was developed for transgenic sexing in Ceratitis capitata (Fu et al., 2007) possibly to overcome the fitness ...

  9. Attraction and Electroantennography responses of the male Mediterranean Fruit Fly, Ceratitis capitata, to natural essential oils and synthetic blends.

    Science.gov (United States)

    Field experiments and long range bioassays were used to understand the difference in attractiveness among various natural essential oils for the Mediterranean Fruit Fly, Ceratitis capitata. Using electroantennography, we have selected various antennally active chemicals and tested their role in the ...

  10. Field estimates of attraction of Ceratitis capitata to Trimedlure and Bactrocera dorsalis (Diptera: Tephritidae) to methyl eugenol in varying environments

    Science.gov (United States)

    Measuring and modeling the attractiveness of semiochemical-baited traps is of significant importance to detection, delimitation and control of invasive pests. Here we describe the results of field mark-release-recapture experiments with Ceratitis capitata (Wiedemann) and Bactrocera dorsalis (Hendel)...

  11. Soil application of Beauveria bassiana to control Ceratitis capitata in semi field conditions.

    Science.gov (United States)

    Ali, Ali; Sermann, Helga; Lerche, Sandra; Büttner, Carmen

    2009-01-01

    The Mediterranean fruit fly Ceratitis capitata (Wiedemann) is a highly polyphagous pest of economic importance cultures in Syria, as in many other parts of the world. The potential of the entomopathogenic fungus Beauveria bassiona BALS (VUIL.) strain 412 against adults of Mediterranean fruit fly C. capitata was evaluated in semi field conditions during the summer. Soil (5-7 cm high) was filled into plastic container (27 cm x 32 cm). In one container 75 pupae, two days before emergency, were spread uniformly on the soil. Then the pupae were covered with soil (4-5 cm layer). After that, 30 ml suspension of fungal spores (4 x 10(8) spores/ml) was applied to the soil surface using a dash bottle. This corresponded to a spore density of 1.3 x 10(7) spores/cm2 on soil. Water and food (1:4 yeast, sucrose) were placed in the cages for the emerged flies. The semi-field evaluation of B. bassiana revealed a fly mortality of about 46% compared to 16% in the control. In addition 72% of dead flies were moulded in the treatment. These results indicated that the entomopathogenic fungus B. bassiana was pathogen against the adults of C. capitata not only in the laboratory condition but also under field condition. That means B. bassiana could decrease the offspring of C. capitata. Therefore B. bassiana could be an effective factor to control C. capitata in combination with other control methods, used in IPM program in the field.

  12. Export of commercial Hass avocados from Argentina poses negligible risk of Ceratitis capitata (Diptera: Tephritidae) infestation.

    Science.gov (United States)

    Villagrán, M Elvira; Willink, Eduardo; Vera, M Teresa; Follett, Peter

    2012-08-01

    Argentina has to meet quarantine restrictions because of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), to export 'Hass' avocados, Persea americana Miller, to certain countries. Hass avocado at the hard, mature green stage is potentially a conditional nonhost for C. capitata and could open export markets without the need for a quarantine treatment. Trapping data from 1998 to 2006 showed that C. capitata was present in avocado orchards, particularly early in the harvest season. The host status of hard, mature green Hass avocado to C. capitata was evaluated using laboratory and field cage tests under no-choice conditions and by assessing natural levels of infestation in commercially harvested fruit from the main avocado production area. In total, 2,250 hard, mature green avocado fruit were exposed to 11,250 gravid females for 24 or 48 h after harvest in laboratory or field cages, and no infestations were found. During 11 seasons, 5,949 fruit in total were sampled from the trees and 992 fruit were collected from the ground, and in none of them were any live or dead fruit fly larvae found. Inspection of >198,000 commercial fruit at the packinghouse from 1998 to 2011 showed no symptoms of fruit fly infestation. These data exceed the published standards for determination of nonhost status, as well as the Probit 9 standard for development of quarantine treatments. Hass avocado harvested at the hard, mature green stage was not infested by C. capitata and seems to pose a negligible quarantine risk. As a consequence, no postharvest treatment or other quarantine actions should be required by importing countries.

  13. Characterisation of novel Bacillus thuringiensis isolates against Aedes aegypti (Diptera: Culicidae) and Ceratitis capitata (Diptera: Tephridae).

    Science.gov (United States)

    Elleuch, Jihen; Tounsi, Slim; Ben Hassen, Najeh Belguith; Lacoix, Marie Noël; Chandre, Fabrice; Jaoua, Samir; Zghal, Raida Zribi

    2015-01-01

    Bacillus thuringiensis is successfully used in pest management strategies as an eco-friendly bioinsecticide. Isolation and identification of new strains with a wide variety of target pests is an ever growing field. In this paper, new B. thuringiensis isolates were investigated to search for original strains active against diptera and able to produce novel toxins that could be used as an alternative for the commercial H14 strain. Biochemical and molecular characterization revealed a remarkable diversity among the studied strains. Using the PCR method, cry4C/Da1, cry30Ea, cry39A, cry40 and cry54 genes were detected in four isolates. Three strains, BLB355, BLB196 and BUPM109, showed feeble activities against Aedes aegypti larvae. Interestingly, spore-crystal mixtures of BLB361, BLB30 and BLB237 were found to be active against Ceratitis capitata with an LC50 value of about 65.375, 51.735 and 42.972 μg cm(-2), respectively. All the studied strains exhibited important mortality levels using culture supernatants against C. capitata larvae. This suggests that these strains produce a wide range of soluble factors active against C. capitata larvae.

  14. Transcriptome analysis in Ceratitis capitata to unveil genes involved in ageing-maturation process

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    V. San Andrés

    2013-07-01

    Full Text Available The sterile insect technique (SIT is widely used in integrated programmes against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann (Diptera: Tephritidae. Information on the age distribution of insects, and more particularly, the knowledge of wild female reproductive status (mature or not at the time of the sterile male release is one of the key factors for the success of the SIT. In recent years, sequencing analysis has become an important tool in molecular biology. In this work we present a genome-wide expression analysis based on SSH (substractive sequence hybridization and EST (expressed sequence tag sequencing and macroarray expression analysis to identify signature genes related to the ageing-maturing process in C. capitata, leading to the successful identification of new putative candidate genes of reproductive status in medfly that would serve as molecular markers for ageing. We have sorted out 94 unigenes from 873 single-pass ESTs, of which 57% have homology with known genes. Ageing-maturing process in C. capitata presents a marked expression pattern accompanied by the increase of transcription level of genes involved in reproduction (vitellogenins, chorion proteins and male-specific serum proteins. Other identified cDNAs (43% with a differential expression pattern would be also candidates but deserve further studies, as they belong to the unknown function class.

  15. Update of host plant list of Anastrepha fraterculus and Ceratitis capitata in Argentina

    Energy Technology Data Exchange (ETDEWEB)

    Orono, Luis E.; Albornoz-Medina, Patricia; Nunez-Campero, Segundo; Nieuwenhove, Guido A. van; Bezdjian, Laura P.; Martin, Cristina B.; Schliserman, Pablo; Ovruski, Sergio M. [Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Tucuman (Argentina). Planta Piloto de Procesos Industriales Microbiologicos y Biotecnologia. Div. Control Biologico de Plagas

    2006-07-01

    The study displays a complete picture of the host range of the two economically important fruit fly species in Argentina, the native Anastrepha fraterculus (Wiedemann) (South American Fruit Fly) and the exotic Ceratitis capitata (Wiedemann) (Mediterranean Fruit Fly or Medfly). This work provides information on the fruit type of each plant species, associated tephritid species, habitat where the fruit was collected, geographical location of each fruit collection area (latitude, longitude, and altitude), phyto geographic regions where each area is located, as well as a general description of the landscape characteristics of those habitats where the fruit samples with fly larvae were collected. A complete, detailed bibliographic review was made in order to provide all the relevant information needed for host use in natural setting. (author)

  16. Selection of Ceratitis capitata (Diptera: Tephritidae) specific recombinant monoclonal phage display antibodies for prey detection analysis.

    Science.gov (United States)

    Monzó, César; Urbaneja, Alberto; Ximénez-Embún, Miguel; García-Fernández, Julia; García, José Luis; Castañera, Pedro

    2012-01-01

    Several recombinant antibodies against the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), one of the most important pests in agriculture worldwide, were selected for the first time from a commercial phage display library of human scFv antibodies. The specificity and sensitivity of the selected recombinant antibodies were compared with that of a rabbit polyclonal serum raised in parallel using a wide range of arthropod species as controls. The selected recombinant monoclonal antibodies had a similar or greater specificity when compared with classical monoclonal antibodies. The selected recombinant antibodies were successfully used to detect the target antigen in the gut of predators and the scFv antibodies were sequenced and compared. These results demonstrate the potential for recombinant scFv antibodies to be used as an alternative to the classical monoclonal antibodies or even molecular probes in the post-mortem analysis studies of generalist predators.

  17. Medfly Ceratitis capitata as Potential Vector for Fire Blight Pathogen Erwinia amylovora: Survival and Transmission.

    Directory of Open Access Journals (Sweden)

    Mónica Ordax

    Full Text Available Monitoring the ability of bacterial plant pathogens to survive in insects is required for elucidating unknown aspects of their epidemiology and for designing appropriate control strategies. Erwinia amylovora is a plant pathogenic bacterium that causes fire blight, a devastating disease in apple and pear commercial orchards. Studies on fire blight spread by insects have mainly focused on pollinating agents, such as honeybees. However, the Mediterranean fruit fly (medfly Ceratitis capitata (Diptera: Tephritidae, one of the most damaging fruit pests worldwide, is also common in pome fruit orchards. The main objective of the study was to investigate whether E. amylovora can survive and be transmitted by the medfly. Our experimental results show: i E. amylovora can survive for at least 8 days inside the digestive tract of the medfly and until 28 days on its external surface, and ii medflies are able to transmit the bacteria from inoculated apples to both detached shoots and pear plants, being the pathogen recovered from lesions in both cases. This is the first report on E. amylovora internalization and survival in/on C. capitata, as well as the experimental transmission of the fire blight pathogen by this insect. Our results suggest that medfly can act as a potential vector for E. amylovora, and expand our knowledge on the possible role of these and other insects in its life cycle.

  18. Population Fluctuation of Ceratitis capitata (Diptera: Tephritidae) as a Function of Altitude in Eastern Guatemala.

    Science.gov (United States)

    Flores, S; Montoya, P; Ruiz-Montoya, L; Villaseñor, A; Valle, A; Enkerlin, W; Liedo, P

    2016-08-01

    Population fluctuations of Ceratitis capitata (Wiedemann) were evaluated over a period of 12 mo in four altitudinal strata (400-750, 750-1,100, 1,100-1,450, and 1,450-1,800 meters above sea level, masl) in Eastern Guatemala. Within each altitudinal range, sampling plots were established in coffee plantations and adjacent areas, in which Jackson traps were set and baited with Trimedlure. Coffee berries and other host fruits were collected. Population density was lowest at the 400-750 masl stratum and highest at 1,450-1,800 masl. At every altitudinal range, the fluctuations of the pest were associated mainly with the availability of ripe coffee berries as a primary host. From 750-1,450 masl, the pest was also associated with the availability of sweet orange and mandarins in commercial and backyard orchards. The highest densities of the pest were recorded in the dry season. Citrus were the main alternate host where ripe coffee berries were not available. This knowledge on population dynamics of C. capitata will contribute to develop more effective area-wide pest management strategies including the use of sterile insects, natural enemies, and bait sprays.

  19. Male-specific phosphorylated SR proteins in adult flies of the Mediterranean fruitfly Ceratitis capitata.

    Science.gov (United States)

    Saccone, Giuseppe; Louis, Christos; Zhang, Hongyou; Petrella, Valeria; Di Natale, Manuela; Perri, Maria; Salvemini, Marco

    2014-01-01

    Alternative splicing is a widely used mechanism of gene regulation in sex determination pathways of Insects. In species from orders as distant as Diptera, Hymenoptera and Coleoptera, female differentiation relies on the activities of conserved splicing regulators, TRA and TRA-2, promoting female-specific expression of the global effector doublesex (dsx). Less understood is to what extent post-translational modifications of splicing regulators plays a role in this pathway. In Drosophila melanogaster phosphorylation of TRA, TRA-2 and the general RBP1 factor by the LAMMER kinase doa (darkener of apricot) is required for proper female sex determination. To explore whether this is a general feature of the pathway we examined sex-specific differences in phosphorylation levels of SR splicing factors in the dipteran species D. melanogaster, Ceratitis capitata (Medfly) and Musca domestica (Housefly). We found a distinct and reproducible pattern of male-specific phosphorylation on protein extracts enriched for SR proteins in C. capitata suggesting that differential phosphorylation may also contribute to the regulation of sex-specific splicing in the Medfly.

  20. Photosensitizing effect of hematoporphyrin IX on immature stages of Ceratitis capitata (Diptera: Tephritidae).

    Science.gov (United States)

    Pujol-Lereis, Luciana Mercedes; Massaldi, Ana; Rabossi, Alejandro; Quesada-Allué, Luis Alberto

    2010-01-01

    Immature stages of Ceratitis capitata were tested as a model for hematoporphyrin IX (HP IX) phototoxicity. The lethal concentration 50 (LC(50)) of HP IX in the food was determined during postembryonic development until adult emergence as 0.173 mm (95% CI: 0.138-0.209). The corresponding HP IX LC(50) during the dispersal period alone was 0.536 mm (95% CI: 0.450-0.633). HP IX toxicity was compared against Phloxine B (PhB) (0.5 mm). HP IX elicited a mortality of 90.87%, which was mainly concentrated during prepupal and early pupal stages. PhB mortality was much lower (56.88%) and occurred mainly during the adult pharate stage. A direct correlation between light-dependent HP IX mortality, evidence of reactive oxygen species (ROS) and lipid peroxidation (conjugated dienes and thiobarbituric acid reactive substances) was established in C. capitata larvae. ROS were found to be very significant in both the brain and in the gut.

  1. The Mediterranean fruit fly (Ceratitis capitata in Iran: genetic diversity and comparison with other countries

    Directory of Open Access Journals (Sweden)

    M. Rajabiyan

    2015-04-01

    Full Text Available The Mediterranean fruit fly, Ceratitis capitata (Wiedemann is an economically important pest on fruits all over the world. The origin of this fly is thought to be from Africa, but it has recently expanded its distribution in many geographic regions including Iran. Due to the wide spread of this pest in Iran and its serious damage to fruit on trees, including citrus orchards of northern Iran, the present study was conducted firstly to investigate genetic diversity within populations of C. capitata based on the sequences of three mitochondrial DNA (mtDNA genes including cytochrome C oxidase I (COI, NAHD dehydrogenase subunits 4 and 5 (ND4 and ND5 and secondly to compare the Iranian haplotypes with those found in other countries. Results of this study indicated low levels of genetic diversity (four, four and three haplotypes among different populations of this pest, respectively for the COI, ND4 and ND5 genes in northern Iranian populations. The genetic similarity and very low levels of genetic diversity of northern Iranian populations suggest that the pest colonisation occurred relatively recently. In addition, haplotypes of Mazandaran province are similar to haplotypes of those countries that have recently been infected by this pest.

  2. Population genetics of Ceratitis capitata in South Africa: implications for dispersal and pest management.

    Science.gov (United States)

    Karsten, Minette; van Vuuren, Bettine Jansen; Barnaud, Adeline; Terblanche, John S

    2013-01-01

    The invasive Mediterranean fruit fly (medfly), Ceratitis capitata, is one of the major agricultural and economical pests globally. Understanding invasion risk and mitigation of medfly in agricultural landscapes requires knowledge of its population structure and dispersal patterns. Here, estimates of dispersal ability are provided in medfly from South Africa at three spatial scales using molecular approaches. Individuals were genotyped at 11 polymorphic microsatellite loci and a subset of individuals were also sequenced for the mitochondrial cytochrome oxidase subunit I gene. Our results show that South African medfly populations are generally characterized by high levels of genetic diversity and limited population differentiation at all spatial scales. This suggests high levels of gene flow among sampling locations. However, natural dispersal in C. capitata has been shown to rarely exceed 10 km. Therefore, documented levels of high gene flow in the present study, even between distant populations (>1600 km), are likely the result of human-mediated dispersal or at least some form of long-distance jump dispersal. These findings may have broad applicability to other global fruit production areas and have significant implications for ongoing pest management practices, such as the sterile insect technique.

  3. Medfly Ceratitis capitata as Potential Vector for Fire Blight Pathogen Erwinia amylovora: Survival and Transmission.

    Science.gov (United States)

    Ordax, Mónica; Piquer-Salcedo, Jaime E; Santander, Ricardo D; Sabater-Muñoz, Beatriz; Biosca, Elena G; López, María M; Marco-Noales, Ester

    2015-01-01

    Monitoring the ability of bacterial plant pathogens to survive in insects is required for elucidating unknown aspects of their epidemiology and for designing appropriate control strategies. Erwinia amylovora is a plant pathogenic bacterium that causes fire blight, a devastating disease in apple and pear commercial orchards. Studies on fire blight spread by insects have mainly focused on pollinating agents, such as honeybees. However, the Mediterranean fruit fly (medfly) Ceratitis capitata (Diptera: Tephritidae), one of the most damaging fruit pests worldwide, is also common in pome fruit orchards. The main objective of the study was to investigate whether E. amylovora can survive and be transmitted by the medfly. Our experimental results show: i) E. amylovora can survive for at least 8 days inside the digestive tract of the medfly and until 28 days on its external surface, and ii) medflies are able to transmit the bacteria from inoculated apples to both detached shoots and pear plants, being the pathogen recovered from lesions in both cases. This is the first report on E. amylovora internalization and survival in/on C. capitata, as well as the experimental transmission of the fire blight pathogen by this insect. Our results suggest that medfly can act as a potential vector for E. amylovora, and expand our knowledge on the possible role of these and other insects in its life cycle.

  4. Morphological characterization of the antennal lobes in the Mediterranean fruit fly Ceratitis capitata.

    Science.gov (United States)

    Solari, Paolo; Corda, Valentina; Sollai, Giorgia; Kreissl, Sabine; Galizia, C Giovanni; Crnjar, Roberto

    2016-02-01

    The medfly Ceratitis capitata is one of the most important pests for horticulture worldwide. The knowledge about anatomy and function of the medfly olfactory system is still limited. The first brain structure to process olfactory information in insects is the antennal lobe (AL), which is composed of its functional and morphological units, the olfactory glomeruli. Here, we present a morphological three-dimensional reconstruction of AL glomeruli in adult brains. We used unilateral antennal backfills of olfactory receptor neurons (ORNs) with neural tracers, revealing the AL structure. We recorded confocal stacks acquired from whole-mount specimens, and analyzed them with the software AMIRA. The ALs in C. capitata are organized in glomeruli which are more tightly packed in the anterior part than the posterior one. Axons of ORNs bilaterally connect the ALs through a commissure between the two ALs. This commissure is formed by several distinct fascicles. Contralateral dye transfer suggests the presence of gap junctions connecting ORNs from both antennae. There was no statistical difference between the average volumes of female ALs (204,166 ± 12,554 μm(3)) and of male ALs (190,287 ± 11,823 μm(3)). In most specimens, we counted 53 glomeruli in each AL, seven of which were sexually dimorphic in size.

  5. Infestation of grape Vitis vinifera by Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) in sub-medium Sao Francisco valley, Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Habibe, Tuffi C.; Viana, Rodrigo E.; Damasceno, Itala Cruz; Malavasi, Aldo [Biofabrica Moscamed Brasil, Juazeiro, BA (Brazil). Distrito Industrial do Sao Francisco; Nascimento, Antonio S., E-mail: antnasc@cnpmf.embrapa.b [EMBRAPA Mandioca e Fruticultura Tropical, Cruz das Almas, BA (Brazil); Paranhos, Beatriz A.J.; Haji, Francisca Nemaura P., E-mail: bjodao@cpatsa.embrapa.b [EMBRAPA Semi-Arido, Petrolina, PE (Brazil); Carvalho, Raimundo S. [Agencia de Defesa Agropecuaria da Bahia (ADAB), Salvador, BA (Brazil)

    2006-07-01

    The objective of this study was to assess the infestation level in grapes, Vitis vinifera, by the medfly,Ceratitis capitata in the Sao Francisco River Valley. The adult population was monitored with Jackson trap baited with trimedlure. Samples of grapes for larval infestation assessment were taken along three months, with a total of 116 kg. The average FTD (flies/trap/day) for medfly males was 0.26. The number of pupae obtained from the fruit samples was 471; 287 adults emerged (60.4%), all Ceratitis capitata. The infestation level was 4.0 pupa/kg of fresh fruit. We conclude that grape is a medfly host in SFV, occasionally causing high damage to production. (author)

  6. One Metarhizium brunneum Strain, Two Uses to Control Ceratitis capitata (Diptera: Tephritidae).

    Science.gov (United States)

    Yousef, M; Garrido-Jurado, I; Quesada-Moraga, E

    2014-10-01

    We determined the virulence and insecticidal activity of the hypocrealean fungus Metarhizium brunneum (Petch) strain EAMb 09/01-Su and its crude extract against Mediterranean fruit fly Ceratitis capitata Wiedemann (Diptera: Tephritidae) and we evaluated the combined use of the fungus with its crude extract. We also determined the effect of fermentation time, temperature, and initial pH of the M. brunneum culture medium on the insecticidal activity of the crude extract. When C. capitata adults were sprayed with a conidial suspension, the strain EAMb 09/01-Su caused 100% mortality with a mean lethal time (LT50) of 5.6 d and mean lethal concentration (LC50) of 2.84 f#x2013; 10(7) conidia per milliliter. Fermentation time significantly affected the lethality of the crude extract when it was provided to C. capitata per os. The highest level of mortality (73.3%) and the shortest median survival time (25.5 h) was obtained from 15-d-old cultures. The crude extract was demonstrated to be thermostable, given that the mortality was >50% at 48 h when the extract had been heated to 100°C for 3 h. Lastly, the optimum initial pH for maximum crude extract activity in terms of mortality ranged between 7 and 9. Additivity was observed for all M. brunneum EAMb 09/01-Su strain crude extract combinations tested, indicating compatibility between products. We concluded that the M. brunneum EAMb 09/01-Su strain is a promising tool for medfly control alone or in combination with its crude extract.

  7. Estimating SIT-driven population reduction in the Mediterranean fruit fly, Ceratitis capitata, from sterile mating.

    Science.gov (United States)

    Juan-Blasco, M; Sabater-Muñoz, B; Pla, I; Argilés, R; Castañera, P; Jacas, J A; Ibáñez-Gual, M V; Urbaneja, A

    2014-04-01

    Area-wide sterile insect technique (SIT) programs assume that offspring reduction of the target population correlates with the mating success of the sterile males released. However, there is a lack of monitoring tools to prove the success of these programs in real-time. Field-cage tests were conducted under the environmental conditions of the Mediterranean coast of Spain to estimate: (a) the mating success of sterile Vienna-8 (V8) Ceratitis capitata males using molecular markers and (b) their efficacy to reduce C. capitata populations under six release ratios of wild females to wild males to V8 males (1:0:0, 1:1:0, 1:1:1, 1:1:5, 1:1:10, and 1:1:20). Statistical models were developed to predict: (a) the number of females captured in traps, (b) sperm ID (sterile or not) in spermathecae of the trapped females, and (c) the viable offspring produced, using release ratio and temperature as predictors. The number of females captured was affected by relative humidity. However, its influence in the model was low. Female captures were significantly higher in ratios 1:0:0 compared to ratios where V8 males were released. The proportion of V8 sperm in spermathecae increased with temperature and with the number of V8 males released, but leveled off between ratios 1:1:10 and 1:1:20. In all seasons, except winter (no offspring), viable offspring increased with temperature and was lowest for ratio 1:1:20. For the first time, a strong negative relationship between proportion of V8 sperm detected by molecular tools and C. capitata offspring was established. The models obtained should contribute to enhance the efficacy of SIT programs against this pest.

  8. Quarantine treatment against mediterranean fruit fly (Ceratitis capitata Wiedemann) in mangoes haden variety (Mangifera indica) with gamma irradiation (Co 60)

    OpenAIRE

    Peña C., María E.; Laboratorio de Bromatología Facultad de Farmacia y Bioquímica de la Universidad Nacional Mayor de San Marcos, Lima, Perú.; Guillen E., Rafael; Servicio Nacional de Sanidad Agraria - SENASA.; Arias A., Gladys C.; Laboratorio de Bromatología Facultad de Farmacia y Bioquímica de la Universidad Nacional Mayor de San Marcos, Lima, Perú.

    2014-01-01

    Tephritidae fruit flies are among the most damaging pests of fruit crops worldwide Ceratitis capitata Wiedemann, for instance, can infest more than 250 fruit and vegetable crop species, and constitutes the main phytosanitary restrict for export purposes. The investigation was carried out in a SENASA’s Production Center of Sterile Fruit Flies and PIMU’s laboratory; it was supervised by an OIEA’s irradiation expert. This research comprised two stages: 1) Determine the minimum irradiation dose t...

  9. Sex and the single embryo: early deveopment in the Mediterranean fruit fly, Ceratitis capitata

    Directory of Open Access Journals (Sweden)

    Zacharopoulou Antigone

    2010-01-01

    Full Text Available Abstract Background In embryos the maternal-to-zygotic transition (MTZ integrates post-transcriptional regulation of maternal transcripts with transcriptional activation of the zygotic genome. Although the molecular mechanisms underlying this event are being clarified in Drosophila melanogaster, little is know about the embryogenic processes in other insect species. The recent publication of expressed sequence tags (ESTs from embryos of the global pest species Ceratitis capitata (medfly has enabled the investigation of embryogenesis in this species and has allowed a comparison of the embryogenic processes in these two related dipteran species, C. capitata and D. melanogaster, that shared a common ancestor 80-100 mya. Results Using a novel PCR-based sexing method, which takes advantage of a putative LTR retrotransposon MITE insertion on the medfly Y chromosome, the transcriptomes of individual early male and female embryos were analysed using RT-PCR. This study is focused on two crucial aspects of the onset of embryonic development: sex determination and cellular blastoderm formation. Together with the three known medfly genes (Cctransformer, Cctransformer2 and Ccdoublesex, the expression patterns of other medfly genes that are similar to the D. melanogaster sex-determination genes (sisterlessA, groucho, deadpan, Sex-lethal, female lethal d, sans fille and intersex and four cellular blastoderm formation genes (Rho1, spaghetti squash, slow-as-molasses and serendipity-α were analyzed, allowing us to sketch a preliminary outline of the embryonic process in the medfly. Furthermore, a putative homologue of the Zelda gene has been considered, which in D. melanogaster encodes a DNA-binding factor responsible for the maternal-to-zygotic transition. Conclusions Our novel sexing method facilitates the study of i when the MTZ transition occurs in males and females of C. capitata, ii when and how the maternal information of "female-development" is

  10. Effects of acoustic waves on pupas of Ceratitis capitata. (Wied., 1824) (Diptera: Tephritidae); Efeitos das ondas acusticas em pupas de Ceratitis capitata. (Wied., 1824) (Diptera: Tephritidae)

    Energy Technology Data Exchange (ETDEWEB)

    Barros, Adilson Camilo de

    2007-07-01

    The aim of this research has been to investigate the hypothesis that acoustic waves would provoke a measurable effect on a population of fruit flies, a treatment denominated sonication. Ionizing radiation, the causative agent for the treatment designated by irradiation has been used as a reference, as long as its effects on living beings and particularly on insects are widely known. This research also enquiries into the possible effects of acoustic waves and gamma rays. The experiments of sonication were carried out in the Laboratory of Entomology of the Instituto Biologico de Sao Paulo. The experiments of radiation were carried out in the Centro de Tecnologia das Radiacoes - IPEN, located within the campus of the University of Sao Paulo. It has been employed a Gamma cell source model 220 with average activity of 757.069 {+-} 293.484 curies (Ci) and an average doses rate of 3.106 {+-} 0.245 kilo grays per hour (kGy/h). Levels applied to the sonication treatment, in hertz and kilo Hertz were: 0 Hz (control) ; 5.0 Hz; 10.0 Hz; 20.0 Hz; 40.0 Hz; 60.0 Hz; 80.0 Hz; 1.0 k Hz; 2.0 k Hz; 10.0 k Hz; 15.0 k Hz e 20.0 k Hz. Irradiation doses applied in Grays were: 5.0 Gy; 7.5 Gy; 10.0 Gy; 12.5 Gy; 15 Gy; 50 Gy; 100 Gy; 150 Gy and 200 Gy. It has been used an acoustic tube made of glass - 40.6 cm in length and 9.1 cm in diameter - and sinusoidal waves originated from three acoustic sources, with response in decibels, which sound intensity varied from 93.60 {+-} 1.51 dB to 123.96 {+-} 0.23 dB. Final results have pointed to evidences that would justify the rejection of null hypothesis H{sub 0}, to which the average of the treatments due to acoustic waves do not differ significantly from each other.(author)

  11. Sniffing out chemosensory genes from the Mediterranean fruit fly, Ceratitis capitata.

    Science.gov (United States)

    Siciliano, Paolo; Scolari, Francesca; Gomulski, Ludvik M; Falchetto, Marco; Manni, Mosè; Gabrieli, Paolo; Field, Linda M; Zhou, Jing-Jiang; Gasperi, Giuliano; Malacrida, Anna R

    2014-01-01

    The Mediterranean fruit fly, Ceratitis capitata (medfly), is an extremely invasive agricultural pest due to its extremely wide host range and its ability to adapt to a broad range of climatic conditions and habitats. Chemosensory behaviour plays an important role in many crucial stages in the life of this insect, such as the detection of pheromone cues during mate pursuit and odorants during host plant localisation. Thus, the analysis of the chemosensory gene repertoire is an important step for the interpretation of the biology of this species and consequently its invasive potential. Moreover, these genes may represent ideal targets for the development of novel, effective control methods and pest population monitoring systems. Expressed sequence tag libraries from C. capitata adult heads, embryos, male accessory glands and testes were screened for sequences encoding putative odorant binding proteins (OBPs). A total of seventeen putative OBP transcripts were identified, corresponding to 13 Classic, three Minus-C and one Plus-C subfamily OBPs. The tissue distributions of the OBP transcripts were assessed by RT-PCR and a subset of five genes with predicted proteins sharing high sequence similarities and close phylogenetic affinities to Drosophila melanogaster pheromone binding protein related proteins (PBPRPs) were characterised in greater detail. Real Time quantitative PCR was used to assess the effects of maturation, mating and time of day on the transcript abundances of the putative PBPRP genes in the principal olfactory organs, the antennae, in males and females. The results of the present study have facilitated the annotation of OBP genes in the recently released medfly genome sequence and represent a significant contribution to the characterisation of the medfly chemosensory repertoire. The identification of these medfly OBPs/PBPRPs permitted evolutionary and functional comparisons with homologous sequences from other tephritids of the genera Bactrocera and

  12. Sniffing out chemosensory genes from the Mediterranean fruit fly, Ceratitis capitata.

    Directory of Open Access Journals (Sweden)

    Paolo Siciliano

    Full Text Available The Mediterranean fruit fly, Ceratitis capitata (medfly, is an extremely invasive agricultural pest due to its extremely wide host range and its ability to adapt to a broad range of climatic conditions and habitats. Chemosensory behaviour plays an important role in many crucial stages in the life of this insect, such as the detection of pheromone cues during mate pursuit and odorants during host plant localisation. Thus, the analysis of the chemosensory gene repertoire is an important step for the interpretation of the biology of this species and consequently its invasive potential. Moreover, these genes may represent ideal targets for the development of novel, effective control methods and pest population monitoring systems. Expressed sequence tag libraries from C. capitata adult heads, embryos, male accessory glands and testes were screened for sequences encoding putative odorant binding proteins (OBPs. A total of seventeen putative OBP transcripts were identified, corresponding to 13 Classic, three Minus-C and one Plus-C subfamily OBPs. The tissue distributions of the OBP transcripts were assessed by RT-PCR and a subset of five genes with predicted proteins sharing high sequence similarities and close phylogenetic affinities to Drosophila melanogaster pheromone binding protein related proteins (PBPRPs were characterised in greater detail. Real Time quantitative PCR was used to assess the effects of maturation, mating and time of day on the transcript abundances of the putative PBPRP genes in the principal olfactory organs, the antennae, in males and females. The results of the present study have facilitated the annotation of OBP genes in the recently released medfly genome sequence and represent a significant contribution to the characterisation of the medfly chemosensory repertoire. The identification of these medfly OBPs/PBPRPs permitted evolutionary and functional comparisons with homologous sequences from other tephritids of the genera

  13. Identification and expression profiling of Ceratitis capitata genes coding for β-hexosaminidases.

    Science.gov (United States)

    Pasini, Maria E; Intra, Jari; Gomulski, Ludvik M; Calvenzani, Valentina; Petroni, Katia; Briani, Federica; Perotti, Maria Elisa

    2011-02-15

    The goal of this study was to identify the genes coding for β-N-acetylhexosaminidases in the Mediterranean fruit fly (medfly) Ceratitis capitata, one of the most destructive agricultural pests, belonging to the Tephritidae family, order Diptera. Two dimeric β-N-acetylhexosaminidases, HEXA and HEXB, have been recently identified on Drosophila sperm. These enzymes are involved in egg binding through interactions with complementary carbohydrates on the surface of the egg shell. Three genes, Hexosaminidase 1 (Hexo1), Hexosaminidase 2 (Hexo2) and fused lobes (fdl), encode for HEXA and HEXB subunits. The availability of C. capitata EST libraries derived from embryos and adult heads allowed us to identify three sequences homologous to the D. melanogaster Hexo1, Hexo2 and fdl genes. Here, we report the expression profile analysis of CcHexo1, CcHexo2 and Ccfdld in several tissues, organs and stages. Ccfdl expression was highest in heads of both sexes and in whole adult females. In the testis and ovary the three genes showed distinct spatial and temporal expression patterns. All the mRNAs were detectable in early stages of spermatogenesis; CcHexo2 and Ccfdl were also expressed in early elongating spermatid cysts. All three genes are expressed in the ovarian nurse cells. CcHexo1 and Ccfdl are stage specific, since they have been observed in stages 12 and 13 during oocyte growth, when programmed cell death occurs in nurse cells. The expression pattern of the three genes in medfly gonads suggests that, as their Drosophila counterparts, they may encode for proteins involved in gametogenesis and fertilization.

  14. An agent-based simulation of extirpation of Ceratitis capitata applied to invasions in California.

    Science.gov (United States)

    Manoukis, Nicholas C; Hoffman, Kevin

    2014-01-01

    We present an agent-based simulation (ABS) of Ceratitis capitata ("Medfly") developed for estimating the time to extirpation of this pest in areas where quarantines and eradication treatments were immediately imposed. We use the ABS, implemented in the program MED-FOES, to study seven different outbreaks that occurred in Southern California from 2008 to 2010. Results are compared with the length of intervention and quarantine imposed by the State, based on a linear developmental model (thermal unit accumulation, or "degree-day"). MED-FOES is a useful tool for invasive species managers as it incorporates more information from the known biology of the Medfly, and includes the important feature of being demographically explicit, providing significant improvements over simple degree-day calculations. While there was general agreement between the length of quarantine by degree-day and the time to extirpation indicated by MED-FOES, the ABS suggests that the margin of safety varies among cases and that in two cases the quarantine may have been excessively long. We also examined changes in the number of individuals over time in MED-FOES and conducted a sensitivity analysis for one of the outbreaks to explore the role of various input parameters on simulation outcomes. While our implementation of the ABS in this work is motivated by C. capitata and takes extirpation as a postulate, the simulation is very flexible and can be used to study a variety of questions on the invasion biology of pest insects and methods proposed to manage or eradicate such species.

  15. Transgenic sexing system for Ceratitis capitata (Diptera: Tephritidae) based on female-specific embryonic lethality.

    Science.gov (United States)

    Ogaugwu, Christian E; Schetelig, Marc F; Wimmer, Ernst A

    2013-01-01

    Fruit fly pest species have been successfully controlled and managed via the Sterile Insect Technique (SIT), a control strategy that uses infertile matings of sterile males to wild females to reduce pest populations. Biological efficiency in the field is higher if only sterile males are released in SIT programs and production costs are also reduced. Sexing strains developed in the Mediterranean fruit fly Ceratitis capitata (medfly) through classical genetics are immensely beneficial to medfly SIT programs but exhibit reduced fertility and fitness. Moreover, transfer of such classical genetic systems to other tephritid species is difficult. Transgenic approaches can overcome this limitation of classical genetic sexing strains (GSSs), but had resulted so far in transgenic sexing strains (TSSs) with dominant lethality at late larval and pupal stages. Here we present a transgene-based female-specific lethality system for early embryonic sexing in medfly. The system utilizes the sex-specifically spliced transformer intron to restrict ectopic mRNA translation of the pro-apoptotic gene hid(Ala5) to females only. The expression of this lethal effector gene is driven by a tetracycline-repressible transactivator gene tTA that is under the control of promoters/enhancers of early-acting cellularization genes. Despite observed position effects on the sex-specific splicing, we could effectively establish this early-acting transgenic sexing system in the medfly C. capitata. After satisfactory performance in large scale tests, TSSs based on this system will offer cost-effective sexing once introduced into SIT programs. Moreover, this approach is straight forward to be developed also for other insect pest and vector species.

  16. Delivery of Nucleic Acids through Embryo Microinjection in the Worldwide Agricultural Pest Insect, Ceratitis capitata.

    Science.gov (United States)

    Gabrieli, Paolo; Scolari, Francesca

    2016-10-01

    The Mediterranean fruit fly (medfly) Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) is a pest species with extremely high agricultural relevance. This is due to its reproductive behavior: females damage the external surface of fruits and vegetables when they lay eggs and the hatched larvae feed on their pulp. Wild C. capitata populations are traditionally controlled through insecticide spraying and/or eco-friendly approaches, the most successful being the Sterile Insect Technique (SIT). The SIT relies on mass-rearing, radiation-based sterilization and field release of males that retain their capacity to mate but are not able to generate fertile progeny. The advent and the subsequent rapid development of biotechnological tools, together with the availability of the medfly genome sequence, has greatly boosted our understanding of the biology of this species. This favored the proliferation of new strategies for genome manipulation, which can be applied to population control. In this context, embryo microinjection plays a dual role in expanding the toolbox for medfly control. The ability to interfere with the function of genes that regulate key biological processes, indeed, expands our understanding of the molecular machinery underlying medfly invasiveness. Furthermore, the ability to achieve germ-line transformation facilitates the production of multiple transgenic strains that can be tested for future field applications in novel SIT settings. Indeed, genetic manipulation can be used to confer desirable traits that can, for example, be used to monitor sterile male performance in the field, or that can result in early life-stage lethality. Here we describe a method to microinject nucleic acids into medfly embryos to achieve these two main goals.

  17. Insecticidal Activity of a Destruxin-Containing Extract of Metarhizium brunneum Against Ceratitis capitata (Diptera: Tephritidae).

    Science.gov (United States)

    Lozano-Tovar, M D; Garrido-Jurado, I; Lafont, F; Quesada-Moraga, E

    2015-04-01

    Tephritid fruit flies are major pests that limit fruit production around the world; they cause important damages, increasing directly and indirectly annual costs, and their management is predominately based on the use of chemical insecticides. This research investigated the insecticidal activity of the crude extract obtained of Metarhizium brunneum Petch EAMb 09/01-Su strain and its capacity to secrete secondary metabolites including destruxins (dtx). Dtx A and A2 had insecticidal activity against Ceratitis capitata (Wiedemann) when administered per os. The crude extract of seven Metarhizium and one Beauveria isolates were evaluated per os against medfly adults. The crude extracts of the isolate EAMb 09/01-Su resulted in mortality ranging between 95 and 100% at 48 h. The high-pressure liquid chromatography profile showed two active peaks (F5B and F6 subfractions) related with dtx A2 and dtx A, which caused 70 and 100% mortality on C. capitata at 48 h postfeeding, respectively. The LC50 was 104.92 ppm of dtx A, contained in the F6 subfraction, and the LT50 was 4.16 h at a concentration of 400 ppm of dtx A contained in the F6 subfraction. Moreover, the average survival time of adults exposed to this subfraction was 12.6 h with only 1 h of exposure. The insecticide metabolites of the F6 subfraction of the EAMb 09/01-Su isolate retained >90% of its insecticidal activity after exposure to 60°C for 2 h and 120°C for 20 min. These results highlight the potential of this strain as a source of new insecticidal compounds of natural origin for fruit fly control.

  18. Pupal development of Ceratitis capitata (Diptera: Tephritidae) and Diachasmimorpha longicaudata (Hymenoptera: Braconidae) at different moisture values in four soil types.

    Science.gov (United States)

    Bento, F de M M; Marques, R N; Costa, M L Z; Walder, J M M; Silva, A P; Parra, J R P

    2010-08-01

    This study aimed to evaluate adult emergence and duration of the pupal stage of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), and emergence of the fruit fly parasitoid, Diachasmimorpha longicaudata (Ashmead), under different moisture conditions in four soil types, using soil water matric potential. Pupal stage duration in C. capitata was influenced differently for males and females. In females, only soil type affected pupal stage duration, which was longer in a clay soil. In males, pupal stage duration was individually influenced by moisture and soil type, with a reduction in pupal stage duration in a heavy clay soil and in a sandy clay, with longer duration in the clay soil. As matric potential decreased, duration of the pupal stage of C. capitata males increased, regardless of soil type. C. capitata emergence was affected by moisture, regardless of soil type, and was higher in drier soils. The emergence of D. longicaudata adults was individually influenced by soil type and moisture factors, and the number of emerged D. longicaudata adults was three times higher in sandy loam and lower in a heavy clay soil. Always, the number of emerged adults was higher at higher moisture conditions. C. capitata and D. longicaudata pupal development was affected by moisture and soil type, which may facilitate pest sampling and allow release areas for the parasitoid to be defined under field conditions.

  19. Transcriptome profiling of sexual maturation and mating in the Mediterranean fruit fly, Ceratitis capitata.

    Directory of Open Access Journals (Sweden)

    Ludvik M Gomulski

    Full Text Available Sexual maturation and mating in insects are generally accompanied by major physiological and behavioural changes. Many of these changes are related to the need to locate a mate and subsequently, in the case of females, to switch from mate searching to oviposition behaviour. The prodigious reproductive capacity of the Mediterranean fruit fly, Ceratitis capitata, is one of the factors that has led to its success as an invasive pest species. To identify the molecular changes related to maturation and mating status in male and female medfly, a microarray-based gene expression approach was used to compare the head transcriptomes of sexually immature, mature virgin, and mated individuals. Attention was focused on the changes in abundance of transcripts related to reproduction, behaviour, sensory perception of chemical stimulus, and immune system processes. Broad transcriptional changes were recorded during female maturation, while post-mating transcriptional changes in females were, by contrast, modest. In male medfly, transcriptional changes were consistent both during maturation and as a consequence of mating. Of particular note was the lack of the mating-induced immune responses that have been recorded for Drosophila melanogaster, that may be due to the different reproductive strategies of these species. This study, in addition to increasing our understanding of the molecular machinery behind maturation and mating in the medfly, has identified important gene targets that might be useful in the future management of this pest.

  20. Oviposition preference hierarchy in Ceratitis capitata (Diptera, Tephritidae: influence of female age and experience

    Directory of Open Access Journals (Sweden)

    Joachim-Bravo Iara S.

    2001-01-01

    Full Text Available The influence of two factors, age and previous experience, on the oviposition hierarchy preference of Ceratitis capitata (Wiedemann, 1824 females was studied. Two populations were analyzed: one reared in laboratory during 17 years and the other captured in nature. In the first experiment the oviposition preference for four fruits, papaya, orange, banana and apple was tested at the beginning of oviposition period and 20 days past. The results showed that the wild females as much the laboratory ones had an oviposition preference hierarchy at the beginning of peak period of oviposition. However this hierarchic preference disappeared in a later phase of life. In the second experiment the females were previously exposed to fruits of different hierarchic positions and afterwards their choice was tested in respect to the oviposition preference for those fruits. The results showed that there was an influence of the previous experience on the posterior choice of fruits to oviposition when the females were exposed to fruits of lower hierarchic position.

  1. Conditional embryonic lethality to improve the sterile insect technique in Ceratitis capitata (Diptera: Tephritidae

    Directory of Open Access Journals (Sweden)

    Franz Gerald

    2009-01-01

    Full Text Available Abstract Background The sterile insect technique (SIT is an environment-friendly method used in area-wide pest management of the Mediterranean fruit fly Ceratitis capitata (Wiedemann; Diptera: Tephritidae. Ionizing radiation used to generate reproductive sterility in the mass-reared populations before release leads to reduction of competitiveness. Results Here, we present a first alternative reproductive sterility system for medfly based on transgenic embryonic lethality. This system is dependent on newly isolated medfly promoter/enhancer elements of cellularization-specifically-expressed genes. These elements act differently in expression strength and their ability to drive lethal effector gene activation. Moreover, position effects strongly influence the efficiency of the system. Out of 60 combinations of driver and effector construct integrations, several lines resulted in larval and pupal lethality with one line showing complete embryonic lethality. This line was highly competitive to wildtype medfly in laboratory and field cage tests. Conclusion The high competitiveness of the transgenic lines and the achieved 100% embryonic lethality causing reproductive sterility without the need of irradiation can improve the efficacy of operational medfly SIT programs.

  2. Transcriptome profiling of sexual maturation and mating in the Mediterranean fruit fly, Ceratitis capitata.

    Science.gov (United States)

    Gomulski, Ludvik M; Dimopoulos, George; Xi, Zhiyong; Scolari, Francesca; Gabrieli, Paolo; Siciliano, Paolo; Clarke, Anthony R; Malacrida, Anna R; Gasperi, Giuliano

    2012-01-01

    Sexual maturation and mating in insects are generally accompanied by major physiological and behavioural changes. Many of these changes are related to the need to locate a mate and subsequently, in the case of females, to switch from mate searching to oviposition behaviour. The prodigious reproductive capacity of the Mediterranean fruit fly, Ceratitis capitata, is one of the factors that has led to its success as an invasive pest species. To identify the molecular changes related to maturation and mating status in male and female medfly, a microarray-based gene expression approach was used to compare the head transcriptomes of sexually immature, mature virgin, and mated individuals. Attention was focused on the changes in abundance of transcripts related to reproduction, behaviour, sensory perception of chemical stimulus, and immune system processes. Broad transcriptional changes were recorded during female maturation, while post-mating transcriptional changes in females were, by contrast, modest. In male medfly, transcriptional changes were consistent both during maturation and as a consequence of mating. Of particular note was the lack of the mating-induced immune responses that have been recorded for Drosophila melanogaster, that may be due to the different reproductive strategies of these species. This study, in addition to increasing our understanding of the molecular machinery behind maturation and mating in the medfly, has identified important gene targets that might be useful in the future management of this pest.

  3. Molecular cloning and expression of nanos in the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae).

    Science.gov (United States)

    Ogaugwu, Christian E; Wimmer, Ernst A

    2013-01-01

    The gene nanos (nos) is a maternal-effect gene that plays an important role in posterior patterning and germ cell development in early stage embryos. nos is known from several diverse insect species, but has so far not been described for any Tephritid fruit fly. Here, we report the molecular cloning and expression pattern of the nos orthologous gene, Ccnos, in the Mediterranean fruit fly Ceratitis capitata, which is a destructive pest of high agricultural importance. CcNOS contains 398 amino acids and has a C-terminal region with two conserved CCHC zinc-binding motifs known to be essential for NOS function. Transcripts of Ccnos were confirmed by in situ hybridization to be maternally-derived and localized to the posterior pole of early stage embryos. Regulatory regions of nos have been employed in genetic engineering in some dipterans such as Drosophila and mosquitoes. Given the similarity in spatial and temporal expression between Ccnos and nos orthologs from other dipterans, its regulatory regions will be valuable to generate additional genetic tools that can be applied for engineering purposes to improve the fight against this devastating pest.

  4. Sperm storage and use in polyandrous females of the globally invasive fruitfly, Ceratitis capitata.

    Science.gov (United States)

    Bertin, Sabrina; Scolari, Francesca; Guglielmino, Carmela R; Bonizzoni, Mariangela; Bonomi, Angelica; Marchini, Daniela; Gomulski, Ludvik M; Gasperi, Giuliano; Malacrida, Anna R; Matessi, Carlo

    2010-11-01

    The medfly, Ceratitis capitata, is an invasive species in which polyandry, associated with sperm precedence, is a common behaviour in the wild. In this species, characterized by internal fertilization, we disclose how the sperm from two males are stored in the female storage organs and how they are used in terms of paternity outcome. The experiments were designed to furnish comparable and unbiased estimates of sperm numbers and progeny in twice-mated females. Results are incorporated in a model through which it is possible to relate the amount of stored sperm with the progeny of twice-mated females. The results show that polyandrous medfly females conserve equal amounts of sperm from the two males to fertilize their eggs. However, we observed a clear advantage of the second male's sperm in siring progeny, which interestingly decreases in favor of the first male as ovipositions progress. The results enable us to exclude differential sperm mortality and suggest that it is the mechanics governing the storage organs which causes the initial, but decreasing second male sperm precedence during the female reproductive life. These outcomes allow us to correlate sperm use in polyandrous females with the mating strategies and invasiveness of this fly.

  5. Thermotolerance and HSP70 expression in the Mediterranean fruit fly Ceratitis capitata.

    Science.gov (United States)

    Kalosaka, Katerina; Soumaka, Elisavet; Politis, Nikos; Mintzas, Anastassios C

    2009-06-01

    The relationship between Hsp70 expression and thermotolerance has been well documented in Drosophila melanogaster. However, there is limited information on this relationship in other insect species. In this report we describe the Hsp70-thermotolerance relationship in one of the major fruit fly pests, Ceratitis capitata (medfly). Hsp70 expression and thermotolerance were assayed at a range of temperatures in several stages of medfly development. The most thermotolerant stage was found to be the late larval stage (100% survival at 41 degrees C) followed by adult flies and late embryos (100% survival at 39 degrees C). These three stages showed a positive relationship between Hsp70 expression and thermotolerance. Mid-larval and mid-embryonic stages were found less thermotolerant and the Hsp70-thermotolerance relationship was not evident. Early embryos did not express Hsp70 at any temperature and exhibited the lowest thermotolerance. The relationship between Hsp70 and inducible thermotolerance was also studied in late larvae. A pretreatment at 37-39 degrees C increased thermotolerance at higher temperatures by approximately 1 degrees C. In parallel, the pretreatment increased Hsp70 expression suggesting a close link between Hsp70 expression and inducible thermotolerance. The increased Hsp70 levels after pretreatment were found to be due to the increased levels of the hsp70 RNA.

  6. Sperm-less males modulate female behaviour in Ceratitis capitata (Diptera: Tephritidae).

    Science.gov (United States)

    Gabrieli, Paolo; Scolari, Francesca; Di Cosimo, Alessandro; Savini, Grazia; Fumagalli, Marco; Gomulski, Ludvik M; Malacrida, Anna R; Gasperi, Giuliano

    2016-12-01

    In the Mediterranean fruit fly, Ceratitis capitata (Wiedemann)(Diptera: Tephritidae), mating has a strong impact on female biology, leading to a decrease in sexual receptivity and increased oviposition and fecundity. Previous studies suggest that sperm transfer may play a role in inducing these behavioural changes. Here we report the identification of a medfly innexin gene, Cc-inx5, whose expression is limited to the germ-line of both sexes. Through RNA interference of this gene, we generated males without testes and, consequently, sperm, but apparently retaining all the other reproductive organs intact. These sperm-less males were able to mate and, like their wild-type counterparts, to induce in their partners increased oviposition rates and refractoriness to remating. Interestingly, matings to sperm-less males results in oviposition rates higher than those induced by copulation with control males. In addition, the observed female post-mating behavioural changes were congruent with changes in transcript abundance of genes known to be regulated by mating in this species. Our results suggest that sperm transfer is not necessary to reduce female sexual receptivity and to increase oviposition and fecundity. These data pave the way to a better understanding of the role/s of seminal components in modulating female post-mating responses. In the long term, this knowledge will be the basis for the development of novel approaches for the manipulation of female fertility, and, consequently, innovative tools to be applied to medfly control strategies in the field.

  7. Influence of protein on feeding behavior of Ceratitis capitata Wiedemann (Diptera: Tephritidae): comparison between immature males and females

    Energy Technology Data Exchange (ETDEWEB)

    Placido-Silva, Maria do C.; Joachim-Bravo, Iara S. [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Inst. de Biologia. Dept. de Biologia Geral; Zucoloto, Fernando S. [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Dept. de Biologia

    2005-07-15

    The objective of this work was to compare the influence of dietary protein on performance and feeding behavior of immature males and females of Ceratitis capitata (Wiedemann). The protein source was beer yeast at 6.5 and 1.5 g.100 ml-1. The following parameters were evaluated: percentage of emergence, total life cycle, adult size, diet consumption, feeding preference and discrimination threshold for yeast. Immature adults showed similar protein requirements regardless of sex. Both males and females showed similar feeding behavior, preferring to feed on the diet with higher protein content. The discrimination threshold for levedure in both sexes was 0.4 g.100 ml-1. We concluded that immature males of C. capitata show similar protein requirements as the immature females. (author)

  8. Cryopreservation of Embryos of the Mediterranean Fruit Fly Ceratitis capitata Vienna 8 Genetic Sexing Strain

    Science.gov (United States)

    Augustinos, Antonios A.; Rajamohan, Arun; Kyritsis, Georgios A.; Zacharopoulou, Antigone; Haq, Ihsan ul; Targovska, Asya; Caceres, Carlos; Bourtzis, Kostas; Abd-Alla, Adly M. M.

    2016-01-01

    The Mediterranean fruit fly, Ceratitis capitata, is one of the most serious pests of fruit crops world-wide. During the last decades, area-wide pest management (AW-IPM) approaches with a sterile insect technique (SIT) component have been used to control populations of this pest in an effective and environment-friendly manner. The development of genetic sexing strains (GSS), such as the Vienna 8 strain, has been played a major role in increasing the efficacy and reducing the cost of SIT programs. However, mass rearing, extensive inbreeding, possible bottleneck phenomena and hitch-hiking effects might pose major risks for deterioration and loss of important genetic characteristics of domesticated insect. In the present study, we present a modified procedure to cryopreserve the embryos of the medfly Vienna 8 GSS based on vitrification and used this strain as insect model to assess the impact of the cryopreservation process on the genetic structure of the cryopreserved insects. Forty-eight hours old embryos, incubated at 24°C, were found to be the most suitable developmental stage for cryopreservation treatment for high production of acceptable hatch rate (38%). Our data suggest the absence of any negative impact of the cryopreservation process on egg hatch rate, pupation rates, adult emergence rates and stability of the temperature sensitive lethal (tsl) character on two established cryopreserved lines (flies emerged from cryopreserved embryos), named V8-118 and V8-228. Taken together, our study provides an optimized procedure to cryopreserve the medfly Vienna 8 GSS and documents the absence of any negative impact on the genetic structure and quality of the strain. Benefits and sceneries for utilization of this technology to support operational SIT projects are discussed in this paper. PMID:27537351

  9. Chill-coma recovery time, age and sex determine lipid profiles in Ceratitis capitata tissues.

    Science.gov (United States)

    Pujol-Lereis, Luciana Mercedes; Fagali, Natalia Soledad; Rabossi, Alejandro; Catalá, Ángel; Quesada-Allué, Luis Alberto

    2016-04-01

    The remodeling of membrane composition by changes in phospholipid head groups and fatty acids (FA) degree of unsaturation has been associated with the maintenance of membrane homeostasis under stress conditions. Overall lipid levels and the composition of cuticle lipids also influence insect stress resistance and tissue protection. In a previous study, we demonstrated differences in survival, behavior and Cu/Zn superoxide dismutase gene expression between subgroups of Ceratitis capitata flies that had a reversible recovery from chill-coma and those that developed chilling-injury. Here, we analyzed lipid profiles from comparable subgroups of 15 and 30-day-old flies separated according to their recovery time after a chill-coma treatment. Neutral and polar lipid classes of chill-coma subgroups were separated by thin layer chromatography and quantified by densitometry. FA composition of polar lipids of chill-coma subgroups and non-stressed flies was evaluated using gas chromatography coupled to mass spectrometry. Higher amounts of neutral lipids such as triglycerides, diacylglycerol, wax esters, sterol esters and free esters were found in male flies that recovered faster from chill-coma compared to slower flies. A multivariate analysis revealed changes in patterns of storage and cuticle lipids among subgroups both in males and females. FA unsaturation increased after cold exposure, and was higher in thorax of slower subgroups compared to faster subgroups. The changes in neutral lipid patterns and FA composition depended on recovery time, sex, age and body-part, and were not specifically associated with the development of chilling-injury. An analysis of phospholipid classes showed that the phosphatidylcholine to lysophosphatidylcholine ratio (PC/LPC) was significantly higher, or showed a tendency, in subgroups that may have developed chilling-injury compared to those with a reversible recovery from coma.

  10. Cryopreservation of Embryos of the Mediterranean Fruit Fly Ceratitis capitata Vienna 8 Genetic Sexing Strain.

    Science.gov (United States)

    Augustinos, Antonios A; Rajamohan, Arun; Kyritsis, Georgios A; Zacharopoulou, Antigone; Haq, Ihsan Ul; Targovska, Asya; Caceres, Carlos; Bourtzis, Kostas; Abd-Alla, Adly M M

    2016-01-01

    The Mediterranean fruit fly, Ceratitis capitata, is one of the most serious pests of fruit crops world-wide. During the last decades, area-wide pest management (AW-IPM) approaches with a sterile insect technique (SIT) component have been used to control populations of this pest in an effective and environment-friendly manner. The development of genetic sexing strains (GSS), such as the Vienna 8 strain, has been played a major role in increasing the efficacy and reducing the cost of SIT programs. However, mass rearing, extensive inbreeding, possible bottleneck phenomena and hitch-hiking effects might pose major risks for deterioration and loss of important genetic characteristics of domesticated insect. In the present study, we present a modified procedure to cryopreserve the embryos of the medfly Vienna 8 GSS based on vitrification and used this strain as insect model to assess the impact of the cryopreservation process on the genetic structure of the cryopreserved insects. Forty-eight hours old embryos, incubated at 24°C, were found to be the most suitable developmental stage for cryopreservation treatment for high production of acceptable hatch rate (38%). Our data suggest the absence of any negative impact of the cryopreservation process on egg hatch rate, pupation rates, adult emergence rates and stability of the temperature sensitive lethal (tsl) character on two established cryopreserved lines (flies emerged from cryopreserved embryos), named V8-118 and V8-228. Taken together, our study provides an optimized procedure to cryopreserve the medfly Vienna 8 GSS and documents the absence of any negative impact on the genetic structure and quality of the strain. Benefits and sceneries for utilization of this technology to support operational SIT projects are discussed in this paper.

  11. Seasonal occurrence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann, 1824 (Diptera: Tephritidae in southern Syria

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    Mansour Mohammed

    2016-09-01

    Full Text Available Population fluctuations of the Mediterranean fruit fly (medfly, Ceratitis capitata, were investigated between 1999 and 2001 at several locations representing fruit production areas in the southern part of Syria (Damascus Ghota, Zabadani, Sargaiah, Rankus, Orneh and Ain Al-Arab. Medfly adults were monitored weekly all year around using Jackson traps baited with trimedlure dispensers. Larvae were also sampled in Damascus Ghota by collecting fruits from ripe or ripening fruit trees and recording the number of larvae emerged from these fruits. In addition, suspected overwintering refuges were sampled at weekly intervals during the three coldest months of the year (December – February and the number of collected larvae was recorded. The results of trap catches and fruit sampling studies showed a similar pattern of occurrence of medfly populations in the study areas, particularly in Damascus Ghota, during the three years of the study. In Damascus Ghota, flies were caught continuously from early June to late December with some variability between years. Two distinct periods of high fly activity were observed: the first one occurred in August and the second in November with a much higher amplitude. In general, seasonal fluctuations in the pattern of occurrence were influenced by differences in temperature and abundance of preferred host fruits. Traps on fig Ficus carica and oriental persimmon Diospyros kaki trees caught the highest numbers of flies, and fruits collected from these trees showed the highest level of infestation, reaching 100% for fig fruit late in the season. Sampling fruits (in Damascus Ghota from trees during the three coldest months of the year showed that a small population of medfly larvae was able to survive winter conditions in prickly pear Opuntia vulgaris fruit left on the trees. In the other areas of the study (Zabadani, Sargaiah, Rankus, Orneh and Ain Al-Arab, only a few flies were caught.

  12. Medhost: An encyclopedic bibliography of the host plants of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann),Version 2.0

    Science.gov (United States)

    MEDHOST,Version 2.0 is the second revision of:"MEDHOST: An encyclopedic bibliography of the host plants of the Mediterranean fruit fly,Ceratitis capitata(Wiedemann),Version 1.0," which was released in 1998 as a Windows-based executable database and listed all plant species reported as hosts of Medit...

  13. Development of phytosanitary cold treatments for oranges infested with Bactrocera invadens and B. zonata (Diptera: Tephritidae) by comparison...existing cold treatment schedules for Ceratitis capitata (Diptera: Tephritidae)

    Science.gov (United States)

    Phytosanitary cold treatments are attempted for Bactrocera invadens Drew, Tsuruta, and White and Bactrocera zonata (Saunders) by comparison with Ceratitis capitata (Wiedemann). Oranges were infested by puncturing holes in the peel and allowing tephritids to oviposit in the holes. The treatments were...

  14. Attraction of Ceratitis capitata (Diptera: Tephritidae) and Nontarget Insects to the Attractant BioLure and its Individual Components in Hawaii.

    Science.gov (United States)

    BioLure, a synthetic food attractant for Mediterranean fruit fly (Ceratitis capitata (Wiedemann)) that uses a combination of three chemical components (ammonium acetate, trimethylamine hydrochloride and putrescine), was deployed in MultiLure traps in predominantly native forests, non-native forests,...

  15. Insecticidal Activity of Basil Oil, trans-Anethole, Estragole, and Linalool to Adults of Ceratitis capitata, Bactrocera dorsalis, and B. cucurbitae

    Science.gov (United States)

    Pest tephritid fruit flies, Ceratitis capitata (Wiedemann), Bactrocera dorsalis (Hendel), and B. cucurbitae (Cocquillett) are among the species of economic significance. Their management has primarily relied on the use of food baits, male attractants and their combinations with insecticides. Basil o...

  16. Capacidad dispersiva de Ceratitis capitata (Diptera: Tephritidae entre valles agrícolas en San Juan, Argentina

    Directory of Open Access Journals (Sweden)

    Leonardo M. DÍAZ

    2008-01-01

    Full Text Available Ceratitis capitata (Wiedemann, díptero tefrítido cosmopolita originario del norte de África, es la principal plaga de los frutales en muchos países de América. El objetivo de este trabajo fue conocer las características de la capacidad de dispersión de Ceratitis capitata , mediante la liberación de moscas estériles en condiciones de campo, con el propósito de predecir las posibles invasiones de valles agrícolas vecinos. Se liberaron 54.000 moscas estériles marcadas de ambos sexos, en localidades del valle de Tulum, cercanas a los ingresos de los valles de Ullum y Zonda. Para el monitoreo del vuelo dispersivo se instalaron trampas Jackson y Mc Phail. Se capturaron 1.213 adultos durante el periodo de muestreo. No hubo propagación de los adultos liberados desde el valle de Tulum a los valles de Ullum y Zonda. La presencia de hospederos, posición geográfica de cada Quebrada y la orientación respecto al viento predominante, podrían explicar la diferencia de difusión entre ambas Quebradas. La dispersión duró cinco semanas. La distancia media de los adultos liberados fue de 3.764 m (S= 2.897 m sin encontrar diferencias entre ambos sexos.

  17. Influence of different tropical fruits on biological and behavioral aspects of the Mediterranean fruit fly Ceratitis capitata (Wiedemann (Diptera, Tephritidae

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    Anne M. Costa

    2011-09-01

    Full Text Available Influence of different tropical fruits on biological and behavioral aspects of the Mediterranean fruit fly Ceratitis capitata (Wiedemann (Diptera, Tephritidae. Studies on Ceratitis capitata, a world fruit pest, can aid the implementation of control programs by determining the plants with higher vulnerability to attacks and plants able to sustain their population in areas of fly distribution. The objective of the present study was to evaluate the influence of eight tropical fruits on the following biological and behavioral parameters of C. capitata: emergence percentage, life cycle duration, adult size, egg production, longevity, fecundity, egg viability, and oviposition acceptance. The fruits tested were: acerola (Malpighia glabra L., cashew (Anacardium occidentale L., star fruit (Averrhoa carambola L., guava (Psidium guajava L., soursop (Annona muricata L., yellow mombin (Spondias mombin L., Malay apple (Syzygium malaccense L., and umbu (Spondias tuberosa L.. The biological parameters were obtained by rearing the recently hatched larvae on each of the fruit kinds. Acceptance of fruits for oviposition experiment was assessed using no-choice tests, as couples were exposed to two pieces of the same fruit. The best performances were obtained with guava, soursop, and star fruit. Larvae reared on cashew and acerola fruits had regular performances. No adults emerged from yellow mombin, Malay apple, or umbu. Fruit species did not affect adult longevity, female fecundity, or egg viability. Guava, soursop, and acerola were preferred for oviposition, followed by star fruit, Malay apple, cashew, and yellow mombin. Oviposition did not occur on umbu. In general, fruits with better larval development were also more accepted for oviposition.Influência de diferentes frutos tropicais em aspectos biológicos e comportamentais da mosca-das-frutas Ceratitis capitata (Wiedemann (Diptera, Tephritidae. Estudos em Ceratitis capitata, uma praga agrícola, pode auxiliar

  18. Influence of different tropical fruits on biological and behavioral aspects of the Mediterranean fruit fly Ceratitis capitata (Wiedemann (Diptera, Tephritidae

    Directory of Open Access Journals (Sweden)

    Anne M. Costa

    2011-09-01

    Full Text Available Influence of different tropical fruits on biological and behavioral aspects of the Mediterranean fruit fly Ceratitis capitata (Wiedemann (Diptera, Tephritidae. Studies on Ceratitis capitata, a world fruit pest, can aid the implementation of control programs by determining the plants with higher vulnerability to attacks and plants able to sustain their population in areas of fly distribution. The objective of the present study was to evaluate the influence of eight tropical fruits on the following biological and behavioral parameters of C. capitata: emergence percentage, life cycle duration, adult size, egg production, longevity, fecundity, egg viability, and oviposition acceptance. The fruits tested were: acerola (Malpighia glabra L., cashew (Anacardium occidentale L., star fruit (Averrhoa carambola L., guava (Psidium guajava L., soursop (Annona muricata L., yellow mombin (Spondias mombin L., Malay apple (Syzygium malaccense L., and umbu (Spondias tuberosa L.. The biological parameters were obtained by rearing the recently hatched larvae on each of the fruit kinds. Acceptance of fruits for oviposition experiment was assessed using no-choice tests, as couples were exposed to two pieces of the same fruit. The best performances were obtained with guava, soursop, and star fruit. Larvae reared on cashew and acerola fruits had regular performances. No adults emerged from yellow mombin, Malay apple, or umbu. Fruit species did not affect adult longevity, female fecundity, or egg viability. Guava, soursop, and acerola were preferred for oviposition, followed by star fruit, Malay apple, cashew, and yellow mombin. Oviposition did not occur on umbu. In general, fruits with better larval development were also more accepted for oviposition.

  19. Genomic structure and expression analysis of the RNase kappa family ortholog gene in the insect Ceratitis capitata.

    Science.gov (United States)

    Rampias, Theodoros N; Fragoulis, Emmanuel G; Sideris, Diamantis C

    2008-12-01

    Cc RNase is the founding member of the recently identified RNase kappa family, which is represented by a single ortholog in a wide range of animal taxonomic groups. Although the precise biological role of this protein is still unknown, it has been shown that the recombinant proteins isolated so far from the insect Ceratitis capitata and from human exhibit ribonucleolytic activity. In this work, we report the genomic organization and molecular evolution of the RNase kappa gene from various animal species, as well as expression analysis of the ortholog gene in C. capitata. The high degree of amino acid sequence similarity, in combination with the fact that exon sizes and intronic positions are extremely conserved among RNase kappa orthologs in 15 diverse genomes from sea anemone to human, imply a very significant biological function for this enzyme. In C. capitata, two forms of RNase kappa mRNA (0.9 and 1.5 kb) with various lengths of 3' UTR were identified as alternative products of a single gene, resulting from the use of different polyadenylation signals. Both transcripts are expressed in all insect tissues and developmental stages. Sequence analysis of the extended region of the longer transcript revealed the existence of three mRNA instability motifs (AUUUA) and five poly(U) tracts, whose functional importance in RNase kappa mRNA decay remains to be explored.

  20. Field Efficacy of a Metarhizium anisopliae-Based Attractant-Contaminant Device to Control Ceratitis capitata (Diptera: Tephritidae).

    Science.gov (United States)

    Navarro-Llopis, V; Ayala, I; Sanchis, J; Primo, J; Moya, P

    2015-08-01

    Biological control of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) using entomopathogenic fungi is being studied as a viable control strategy. The efficacy of a Metarhizium anisopliae (Metschnikoff) Sorokin (Hypocreales: Clavicipitaceae)-based attractant-contaminant device (ACD) to control C. capitata was evaluated in a medium-scale (40 ha) 2-yr field trial using a density of 24 ACD per ha. Results showed that this density was adequate to efficiently reduce fruitfly populations and that the inoculation dishes (IDs) needed replacing mid-season to provide protection for the entire season. In this study, fungal treatment was even more effective than conventional chemical treatment. Population dynamics in fungus-treated fields along with the infectivity study of field-aged IDs in the laboratory found that the ACD remained effective for at least 3 mo. The results suggest M. anisopliae-based ACD can be used to control C. capitata in the field. The implications of its use, especially as a tool in an integrated pest management program, are discussed.

  1. Ammonium Acetate Enhances the Attractiveness of a Variety of Protein-Based Baits to Female Ceratitis capitata (Diptera: Tephritidae).

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    Piñero, Jaime C; Souder, Steven K; Smith, Trevor R; Fox, Abbie J; Vargas, Roger I

    2015-04-01

    Ammonia and its derivatives are used by female fruit flies (Diptera: Tephritidae) as volatile cues to locate protein-rich food needed to produce their eggs. This need for external protein sources has led to the development of behaviorally based control strategies such as food-based lures and insecticidal baits targeting pestiferous fruit fly species. In field cage studies conducted in Hawaii, we examined the behavioral response of laboratory-reared male and female Mediterranean fruit fly, Ceratitis capitata (Wiedemann), to seven commercially available protein baits and to beer waste, a relatively inexpensive and readily available substance. Each material was tested alone or in combination with either ammonium acetate or ammonium carbonate. For the majority of baits evaluated, the presence of ammonium acetate, but not ammonium carbonate, elicited a significantly greater level of response of female C. capitata compared with the protein baits alone. The addition of ammonium acetate to selected baits increased bait attractiveness to a level comparable with that elicited by the most widely used spinosad-based protein bait, GF-120. Our findings indicate that the addition of ammonium acetate to commercially available proteinaceous baits and to beer waste can greatly improve their attractiveness to C. capitata, potentially increasing the bait's effectiveness for fruit fly monitoring and suppression.

  2. Flutuação populacional de Anastrepha fraterculus (Wiedemann e Ceratitis capitata (Wiedemann (Diptera, Tephritidae em pomares de pessegueiro em Porto Alegre, Rio Grande do Sul Population fluctuation of Anastrepha fraterculus (Wiedemann and Ceratitis capitata (Wiedemann (Diptera, Tephritidae in peach orchards in Porto Alegre, Rio Grande do Sul

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    Flávio Roberto Mello Garcia

    1998-01-01

    Full Text Available This paper presents the study of population fluctuation of Anastrepha fraterculus (Wiedemann, 1824 and Ceratitis capitata (Wiedemann, 1830 in peach orchards in Porto Alegre city. The peak for A. fraterculus was in November and December and for C. capitata in December and January. There was no significant difference among the population levels in the cultivars Fla 13-72, Premier and Marli.

  3. Development of phytosanitary cold treatments for oranges infested with Bactrocera invadens and Bactrocera zonata (Diptera: Tephritidae) by comparison with existing cold treatment schedules for Ceratitis capitata (Diptera: Tephritidae).

    Science.gov (United States)

    Hallman, Guy J; Myers, Scott W; El-Wakkad, Mokhtar F; Tadrous, Meshil D; Jessup, Andrew J

    2013-08-01

    Phytosanitary cold treatments were tested for Bactrocera invadens Drew, Tsuruta, and White and Bactrocera zonata (Saunders) using comparisons with Ceratitis capitata (Wiedemann). Oranges were infested by puncturing holes in the peel and allowing tephritids to oviposit in the holes. The treatments were initiated when the larvae reached late third instar because previous research had shown that stage to be the most cold tolerant for all three species. Results show that B. invadens is not more cold tolerant than C. capitata and B. zonata at 1.0 +/- 0.1 degrees C and lend support to the use of C. capitata cold treatment schedules for B. invadens. It cannot be concluded that B. zonata is not more cold tolerant than C. capitata.

  4. Solid phase microextraction of volatile emissions of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae): influence of fly sex, age, and mating status.

    Science.gov (United States)

    Alfaro, Cristina; Vacas, Sandra; Zarzo, Manuel; Navarro-Llopis, Vicente; Primo, Jaime

    2011-01-12

    Considerable efforts have been devoted to understanding the courtship behavior and pheromone communication of medflies; however, the sex pheromone composition is still a controversial subject. The discovery of new components affecting medfly behavior would be of interest for medfly control methods based on semiochemicals. This work describes volatile compounds emitted by Ceratitis capitata collected using solid phase microextraction. The volatile study was conducted according to an experimental design with three factors (sex, age, and mating status) assumed to be relevant for better understanding the chemical communication. Emission data were treated by means of principal component analysis, a statistical methodology not previously applied to the study of volatiles emitted by fruit flies. The characterization of emission patterns could be useful for the selection of compounds to be further investigated in biological assays to improve knowledge of the key semiochemicals involved in medfly behavior.

  5. How functional genomics will impact fruit fly pest control: the example of the Mediterranean fruit fly, Ceratitis capitata.

    Science.gov (United States)

    Scolari, Francesca; Gomulski, Ludvik M; Gabrieli, Paolo; Manni, Mosè; Savini, Grazia; Gasperi, Giuliano; Malacrida, Anna R

    2014-01-01

    The highly invasive agricultural insect pest Ceratitis capitata (Diptera: Tephritidae) is the most thoroughly studied tephritid fruit fly at the genetic and molecular levels. It has become a model for the analysis of fruit fly invasions and for the development of area-wide integrated pest management (AW-IPM) programmes based on the environmentally-friendly Sterile Insect Technique (SIT). Extensive transcriptome resources and the recently released genome sequence are making it possible to unravel several aspects of the medfly reproductive biology and behaviour, opening new opportunities for comparative genomics and barcoding for species identification. New genes, promotors and regulatory sequences are becoming available for the development/improvement of highly competitive sexing strains, for the monitoring of sterile males released in the field and for determining the mating status of wild females. The tools developed in this species have been transferred to other tephritids that are also the subject of SIT programmes.

  6. Mating choice of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae): influence of male ageing on mating success; Escolha de parceiro para acasalamento em Ceratitis capitata (Wiedemann)(Diptera: Tephritidae): influencia do envelhecimento dos machos no sucesso de copula

    Energy Technology Data Exchange (ETDEWEB)

    Silva Neto, Alberto M. da; Dias, Vanessa S.; Joachim-Bravo, Iara S. [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Inst. de Biologia. Dept. de Biologia Geral], e-mail: bio.alberto@gmail.com, e-mail: vanessasidias@hotmail.com, e-mail: ibravo@ufba.br

    2009-09-15

    The objective of this study was to evaluate the effect of male ageing on male pheromone release and mating success of Ceratitis capitata (Wiedemann). The effects of male ageing on mating were evaluated on fi ve and 21 d-old males by assessing their mating success (males chosen by a female for copulation) and the amount of males releasing the sex pheromone. The mating success was evaluated by using several ratios of young to older males by increasing the number of older males:young males from 1:1 to 5:1. The mating success of the 1:1 ratio was also evaluated in fi eld cages. The evaluation of the mating success (in the 1:1 ratio) showed a clear preference of the females for young males. Sex pheromone emission was much more common on young than older males. Even in cases were older males were more abundant (ratios 2:1 and 3:1), females still chose the young males. However, females could not distinguish young from older males in ratios of 4:1 or 5:1. Our data indicate that the ageing of C. capitata males has a considerable negative effect on their reproductive success, especially if they are found in a proportion any lower than 3:1. (author)

  7. Transcriptional profiles of mating-responsive genes from testes and male accessory glands of the Mediterranean fruit fly, Ceratitis capitata.

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    Francesca Scolari

    Full Text Available BACKGROUND: Insect seminal fluid is a complex mixture of proteins, carbohydrates and lipids, produced in the male reproductive tract. This seminal fluid is transferred together with the spermatozoa during mating and induces post-mating changes in the female. Molecular characterization of seminal fluid proteins in the Mediterranean fruit fly, Ceratitis capitata, is limited, although studies suggest that some of these proteins are biologically active. METHODOLOGY/PRINCIPAL FINDINGS: We report on the functional annotation of 5914 high quality expressed sequence tags (ESTs from the testes and male accessory glands, to identify transcripts encoding putative secreted peptides that might elicit post-mating responses in females. The ESTs were assembled into 3344 contigs, of which over 33% produced no hits against the nr database, and thus may represent novel or rapidly evolving sequences. Extraction of the coding sequences resulted in a total of 3371 putative peptides. The annotated dataset is available as a hyperlinked spreadsheet. Four hundred peptides were identified with putative secretory activity, including odorant binding proteins, protease inhibitor domain-containing peptides, antigen 5 proteins, mucins, and immunity-related sequences. Quantitative RT-PCR-based analyses of a subset of putative secretory protein-encoding transcripts from accessory glands indicated changes in their abundance after one or more copulations when compared to virgin males of the same age. These changes in abundance, particularly evident after the third mating, may be related to the requirement to replenish proteins to be transferred to the female. CONCLUSIONS/SIGNIFICANCE: We have developed the first large-scale dataset for novel studies on functions and processes associated with the reproductive biology of Ceratitis capitata. The identified genes may help study genome evolution, in light of the high adaptive potential of the medfly. In addition, studies of male

  8. Size relationships of different body parts in the three dipteran species Drosophila melanogaster, Ceratitis capitata and Musca domestica.

    Science.gov (United States)

    Siomava, Natalia; Wimmer, Ernst A; Posnien, Nico

    2016-06-01

    Body size is an integral feature of an organism that influences many aspects of life such as fecundity, life span and mating success. Size of individual organs and the entire body size represent quantitative traits with a large reaction norm, which are influenced by various environmental factors. In the model system Drosophila melanogaster, pupal size and adult traits, such as tibia and thorax length or wing size, accurately estimate the overall body size. However, it is unclear whether these traits can be used in other flies. Therefore, we studied changes in size of pupae and adult organs in response to different rearing temperatures and densities for D. melanogaster, Ceratitis capitata and Musca domestica. We confirm a clear sexual size dimorphism (SSD) for Drosophila and show that the SSD is less uniform in the other species. Moreover, the size response to changing growth conditions is sex dependent. Comparison of static and evolutionary allometries of the studied traits revealed that response to the same environmental variable is genotype specific but has similarities between species of the same order. We conclude that the value of adult traits as estimators of the absolute body size may differ among species and the use of a single trait may result in wrong assumptions. Therefore, we suggest using a body size coefficient computed from several individual measurements. Our data is of special importance for monitoring activities of natural populations of the three dipteran flies, since they are harmful species causing economical damage (Drosophila, Ceratitis) or transferring diseases (Musca).

  9. Insecticidal effects of various concentrations of selected extractions of Cestrum parqui on adult and immature Ceratitis capitata.

    Science.gov (United States)

    Zapata, Nelson; Budia, Flor; Viñuela, Elisa; Medina, Pilar

    2006-04-01

    Aqueous extracts of Cestrum parqui L'Héritier (Solanaceae) were evaluated at different concentrations in several stages of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae). For adults, the study was extended to extracts obtained with several solvents of an increasing degree of polarity. Aqueous extracts from C. parqui showed a high toxicity to neonate larvae when ingested through diet, inhibiting pupation at a concentration above 0.6%. Lower concentrations delayed the larval development and reduced the percentages of pupae formed and adult emergence. An LC50 = 0.9% after 3 d of continuous ingestion of C. parqui aqueous extracts could be calculated, whereas extracts obtained with organic compounds were nearly innocuous except with the use of the solvent methanol/water (80:20), the more polar of those tested, that killed 12.5% of adults. Aqueous extracts were also harmful to adults by diminishing the reproductive potential, which implies a significant effect on the offspring. Egg contact with insecticide or dipping third instars did not cause any adverse effect, supporting the hypothesis that only by means of ingestion can the toxic compounds of C. parqui reach the target. Our results showed that C. parqui components causing C. capitata mortality are mostly dissolved in water and not in organic solvents, which point out their polar character.

  10. Intensity of Attack Caused by Mediterranean Fruit Fly Ceratitis capitata Wiedem. (Diptera, Tephritidae on Mandarin along the Montenegrin Seacoast

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    Sanja Radonjić

    2011-01-01

    Full Text Available Along the Montenegrin seacoast, all cultivated citrus species (mandarin, orange, lemon,grapefruit, fig, loquat and ziziphus were detected as host plants of the Ceratitis capitata Wiedem.Among those found, in economic sense, the most important host plant is mandarinUnšiu.Intensity of the attack were monitored on mandarin in 2003 and 2004 in localities Baošići,Lastva Grbaljska and Bar, and was determined as higher in 2003. The first symptoms ofattack were detected, depending on locality, from middle of September until beginning ofOctober, one to two months earlier then in 2004. The maximum number of larvae per fruitwere detected in October and November (average from 13.44±0.16 to 22.82±0.13. Timeof the first symptoms appearence on mandarin in September and October 2003 and in November2004, indicate on crucial importance of alternative host plants (figs and loquat forreproduction and increasing of C. capitata population, because later during a season its intensityof attack on mandarin, depend largely on their presence.

  11. Field Estimates of Attraction of Ceratitis capitata to Trimedlure and Bactrocera dorsalis (Diptera: Tephritidae) to Methyl Eugenol in Varying Environments.

    Science.gov (United States)

    Manoukis, Nicholas C; Siderhurst, Matthew; Jang, Eric B

    2015-06-01

    Measuring and modeling the attractiveness of semiochemical-baited traps is of significant importance to detection, delimitation, and control of invasive pests. Here, we describe the results of field mark-release-recapture experiments with Ceratitis capitata (Wiedemann) and Bactrocera dorsalis (Hendel) to estimate the relationship between distance from a trap baited with trimedlure and methyl eugenol, respectively, and probability of capture for a receptive male insect. Experiments were conducted using a grid of traps with a central release point at two sites on Hawaii Island, a Macadamia orchard on the East side of the island and a lava field on the West side. We found that for B. dorsalis and methyl eugenol there is a 65% probability of capture at ∼36 m from a single trap, regardless of habitat. For C. capitata, we found a 65% probability of capture at a distance of ∼14 m from a single trap in the orchard and 7 m in the lava field. We also present results on the spatial and temporal pattern of recaptures. The attraction data are analyzed via a hyperbolic secant-based capture probability model.

  12. Wing Morphometry and Acoustic Signals in Sterile and Wild Males: Implications for Mating Success in Ceratitis capitata.

    Science.gov (United States)

    de Souza, João Maria Gomes Alencar; de Lima-Filho, Paulo Augusto; Molina, Wagner Franco; de Almeida, Lúcia Maria; de Gouveia, Milson Bezerra; de Macêdo, Francisco Pepino; Laumann, Raul Alberto; Paranhos, Beatriz Aguiar Jordão

    2015-01-01

    The sterile insect technique (SIT) is widely utilized in the biological control of fruit flies of the family Tephritidae, particularly against the Mediterranean fruit fly. This study investigated the interaction between mating success and morphometric variation in the wings and the production of acoustic signals among three male groups of Ceratitis capitata (Wiedemann): (1) wild males, (2) irradiated with Co-60 (steriles), and (3) irradiated (steriles) and treated with ginger oil. The canonical variate analysis discriminated two groups (males irradiated and males wild), based on the morphological shape of the wings. Among males that emit buzz signals, wild males obtained copulation more frequently than males in Groups 2 and 3. The individuals of Group 3 achieved more matings than those in Group 2. Wild males displayed lower pulse duration, higher intervals between pulses, and higher dominant frequency. Regarding the reproductive success, the morphological differences in the wings' shape between accepted and nonaccepted males are higher in wild males than in the irradiated ones. The present results can be useful in programs using the sterile insect technique for biological control of C. capitata.

  13. Cloning and purification of protein kinase CK2 recombinant alpha and beta subunits from the Mediterranean fly Ceratitis capitata.

    Science.gov (United States)

    Kouyanou-Koutsoukou, Sophia; Baier, Andrea; Kolaitis, Regina-Maria; Maniatopoulou, Evanthia; Thanopoulou, Konstantina; Szyszka, Ryszard

    2011-10-01

    The Mediterranean fruit fly Ceratitis capitata is an insect capable of wreaking extensive damage to a wide range of fruit crops. Protein kinase CK2 is a ubiquitous Ser/Thr kinase that is highly conserved among eukaryotes; it is a heterotetramer composed of two catalytic (α) and a dimer of regulatory (β) subunits. We present here the construction of the cDNA molecules of the CK2α and CK2β subunits from the medfly C. capitata by the 5'/3' RACE and RT-PCR methods, respectively. CcCK2α catalytic subunit presents the characteristic and conserved features of a typical protein kinase, similar to the regulatory CcCK2β subunit, that also possess the conserved features of regulatory CK2β subunits, as revealed by comparison of their predicted amino acid sequences with other eukaryotic species. The recombinant CcCK2α and CcCK2β proteins were purified by affinity chromatography to homogeneity, after overexpression in Escherichia coli. CcCK2α is capable to utilize GTP and its activity and is inhibited by polyanions and stimulated by polycations in phosphorylation assays, using purified acidic ribosomal protein P1 as a substrate.

  14. 地中海实蝇幼虫分子鉴定%Molecular identification for larvae of Ceratitis capitata

    Institute of Scientific and Technical Information of China (English)

    叶军; 周国梁; 易建平; 吴家教; 阮长浩; 梁帆; 郑建中; 王文兵

    2007-01-01

    通过对从秘鲁进口的葡萄中截获实蝇类幼虫进行ITS区和线粒体CO Ⅰ,COⅡ,COⅢ、ND5基因序列的扩增和测序,并与GenBank中对应的序列进行比对,结果表明,截获样品ITS区序列和地中海实蝇Ceratitis capitata(Wiedemann)同源性为95.16%(其中ITS1为99.52%,ITS2为86.2%),线粒体COⅠ,COⅡ,COⅢ,ND5基因序列和地中海实蝇C.capitata同源性为100%,99.9%,99.5%,99.8%;基于CO Ⅰ序列构建的系统发育树中,幼虫样品和地中海实蝇最为接近.根据序列分析和系统发育关系分析的结果,将截获的实蝇类幼虫鉴定为地中海实蝇C.capitata.

  15. Cloning, sequence identification and expression profile analysis of α-L-fucosidase gene from the Mediterranean fruit fly Ceratitis capitata.

    Science.gov (United States)

    Intra, Jari; Perotti, Maria-Elisa; Pasini, Maria Enrica

    2011-04-01

    The Mediterranean fruit fly Ceratitis capitata (Diptera: Tephritidae) is one of the most destructive agricultural pests, a polyphagus insect of relevant economic importance and is widespread in many regions around the world. It is the best-studied fruit fly pest at genetic and molecular level and much has been learned on its ecology and behaviour. An α-L-fucosidase has been recently hypothesized to be involved in sperm-egg interactions in Drosophila melanogaster and in other Drosophila species. Here, a complete cDNA encoding a putative α-L-fucosidase of the medfly was amplified using the reverse polymerase chain reaction (RT-PCR) with degenerate based on the conserved coding sequence information of several insect α-L-fucosidases, cloned and sequenced (GenBank accession no. FJ177429). The coding region consisted of 1482 bp which encoded a 485-residues protein (named CcFUCA) with a predicted molecular mass of 56.1 kDa. The deduced protein sequence showed 75% amino acid identity to D. melanogaster α-L-fucosidase, and in fact the phylogenetic tree analysis revealed that CcFUCA had closer relationships with the α-L-fucosidases of drosophilid species. The tissue expression analysis indicated that CcFuca was expressed in a single transcript in all tissues, suggesting a ubiquitous localization pattern of the encoded protein. Our findings provide novel insights on a gene encoding a protein potentially involved in primary gamete interactions in C. capitata.

  16. Wing Morphometry and Acoustic Signals in Sterile and Wild Males: Implications for Mating Success in Ceratitis capitata

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    João Maria Gomes Alencar de Souza

    2015-01-01

    Full Text Available The sterile insect technique (SIT is widely utilized in the biological control of fruit flies of the family Tephritidae, particularly against the Mediterranean fruit fly. This study investigated the interaction between mating success and morphometric variation in the wings and the production of acoustic signals among three male groups of Ceratitis capitata (Wiedemann: (1 wild males, (2 irradiated with Co-60 (steriles, and (3 irradiated (steriles and treated with ginger oil. The canonical variate analysis discriminated two groups (males irradiated and males wild, based on the morphological shape of the wings. Among males that emit buzz signals, wild males obtained copulation more frequently than males in Groups 2 and 3. The individuals of Group 3 achieved more matings than those in Group 2. Wild males displayed lower pulse duration, higher intervals between pulses, and higher dominant frequency. Regarding the reproductive success, the morphological differences in the wings’ shape between accepted and nonaccepted males are higher in wild males than in the irradiated ones. The present results can be useful in programs using the sterile insect technique for biological control of C. capitata.

  17. Toxicity of naturally occurring Bio-fly and chitosan compounds to control the Mediterranean fruit fly Ceratitis capitata (Wiedemann).

    Science.gov (United States)

    Rabea, E I; Nasr, H M; Badawy, M E I; El-Gendy, I R

    2015-01-01

    The efficacy of five compounds of a biopolymer chitosan and Bio-fly (Beauveria bassiana fungus) as biopesticide was evaluated on Ceratitis capitata under laboratory conditions. The inhibitory effects on acetylcholinesterase (AChE) and adenosinetriphosphatase (ATPase) as biochemical indicators were also determined in vivo. The results indicated that B. bassiana based Bio-fly exhibited significant toxicity against C. capitata (LC50 = 3008 and 3126 mg/L after 48 h in females and males, respectively) followed by the derivatives of chitosan, N-(4-propylbenzyl)chitosan and N-(2-nitrobenzyl)chitosan. Bio-fly displayed remarkable inhibition of AChE activity (IC50 = 2220 mg/L) while N-(2-chloro,6-flourobenzyl)chitosan, N-(4-propylbenzyl)chitosan and N-(3,4-methylenedioxybenzyl) chitosan had no significant difference in inhibitory action. In adult males, N-(2-nitrobenzyl)chitosan exhibited the highest inhibitory action (IC50 = 6569 mg/L). In addition, the toxic effects of the tested compounds on the activity of ATPase indicated that highly significant inhibition was found with N-(4-propylbenzyl)chitosan with an IC50 of 8194 and 8035 mg/L, in females and males, respectively.

  18. Evaluación de colores para la oviposición de Ceratitis capitata (Diptera, Tephritidae en Argentina

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    Lorena Suárez

    2013-04-01

    Full Text Available Ceratitis capitata es una de las principales plagas de la fruticultura mundial y en Argentina es la Mosca de los Frutos de mayor abundancia y distribución. El desarrollo de una trampa artificial de oviposición, puede ser una herramienta útil de monitoreo para hembras grávidas. El objetivo de este trabajo fue evaluar la capacidad atrayente para la oviposición de C. capitata sobre esferas de agar de color amarillo, rojo, verde, negro y sin color; en condiciones de laboratorio. Se observaron diferencias estadísticamente significativas, siendo las esferas rojas y negras las más atractivas sobre las verdes, amarillas y sin color. En laboratorio, la mayor atracción alcanzada por el rojo y negro sustentaría la hipótesis de que el fuerte contraste respecto al fondo incrementaría la atracción de los colores más oscuros.

  19. Nitric oxide synthase-dependent NADPH-diaphorase activity in the optic lobes of male and female Ceratitis capitata mutants

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    E Roda

    2009-06-01

    Full Text Available Nitric oxide (NO is acknowledged as a messenger molecule in the nervous system with a pivotal role in the modulation of the chemosensory information. It has been shown to be present in the optic lobes of several insect species. In the present study, we used males and females from four different strains of the medfly Ceratitis capitata (Diptera, Tephritidae: or; or,wp (both orange eyed; w,M360 and w,Heraklion (both white eyed, as models to further clarify the involvement of NO in the mutants’ visual system and differences in its activity and localization in the sexes. Comparison of the localization pattern of NO synthase (NOS, through NADPH-diaphorase (NADPHd staining, in the optic lobes of the four strains, revealed a stronger reaction intensity in the retina and in the neuropile region lamina than in medulla and lobula. Interestingly, the intensity of NADPHd staining differs, at least in some strains, in the optic lobes of the two sexes; all the areas are generally strongly labelled in the males of the or and w,M360 strains, whereas the w,Heraklion and or,wp mutants do not show evident sexdependent NADPHd staining. Taken as a whole, our data point to NO as a likely transmitter candidate in the visual information processes in insects, with a possible correlation among NOS distribution, eye pigmentation and visual function in C. capitata males. Moreover, NO could influence behavioural differences linked to vision in the two sexes.

  20. Pathogenicity of Beauveria bassiana isolated from Moroccan Argan forests soil against larvae of Ceratitis capitata (Diptera: Tephritidae) in laboratory conditions.

    Science.gov (United States)

    Imoulan, Abdessamad; Elmeziane, Abdellatif

    2014-03-01

    The Mediterranean fruit fly, Ceratitis capitata Wiedemann (Diptera: Tephritidae), is the major tephritid pest in Morocco. This pest survives in Moroccan forests Argania spinosa and continually invades the nearest agricultural areas. Entomopathogenic fungi are an interesting tool for fruit fly control and hold a useful alternative to conventional insecticides. However, primary selection of effective pathogens should be taken in laboratory condition prior to applying them in the field. Here, we used third late instar larvae of C. capitata to investigate the effectiveness of 15 local Beauveria bassiana isolates. Results showed that all isolates were able to infect the larval stage, producing a large mortality rate in puparia ranging from 65 to 95 % and caused significant reduction in adult emergence. The fungal treatments revealed that the mycosis occurred also in adults escaping infection as pupariating larvae. The percentage of mycosed puparia was highest in strain TAM6.2 (95 %) followed by ERS4.16 (90 %), therefore they were the most virulent. Median lethal concentration (LC₅₀) was studied for five isolates at four concentrations ranging from 10⁵ to 10⁸ conidia ml⁻¹. The results showed that the slopes of regression lines for B. bassiana ERS4.16 (slope = 0.386) and TAM6.2 (slope = 0.41) were the most important and had the lowest LC₅₀ values (2.85 × 10³ and 3.16 × 10³ conidia ml⁻¹ respectively). This investigation suggests that the soil of Argan forests contains pathogenic B. bassiana isolates and highlights for the first time their potential as biological control toward C. capitata larval stage in Morocco.

  1. Sublethal effect of neem extract on mediterranean fruit fly adults Efeito subletal de extratos de Azadirachta indica Sobre adultos de Ceratitis capitata

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    Márcio Alves Silva

    2013-03-01

    Full Text Available The sublethal effect of extracts of Azadirachta indica on Ceratitis capitata was evaluated. Two pairs of flies were treated in plastic tubes with cotton placed in plastic cages. An artificial diet (hydrolyzed protein + sugar was provided ad libitum. The extracts affected significantly the longevity of C. capitata. The pre-oviposition period were not significantly affected by the extracts. The A. indica branches extracted with dichloromethane (888 ppm affected significantly the fecundity and fertility, reducing the number of eggs laid to approximately 80 % and the egg hatching by 30 % at the 8th day. Therefore, the neem branches extracted with dichloromethane affected the reproduction of C. capitata.Neste estudo, avaliou-se o efeito subletal de extratos de Azadirachta indica sobre Ceratitis capitata. Os tratamentos em um tubo plástico com algodão foram expostos para dois casais numa gaiola plástica. Nessa gaiola, também foi fornecida dieta artificial (proteína hidrolisada + açúcar ad libitum. Os extratos afetaram significativamente a longevidade de C. capitata. O período de pré-oviposição não foi significativamente afetado pelos extratos. Em contrapartida, o extrato de ramos em diclorometano (888 ppm afetou a fecundidade e a fertilidade, reduzindo em 80% o número de ovos colocados e a viabilidade dos ovos em 30%, no 8º dia. Portanto, o extrato de ramos em diclorometano afeta a reprodução de C. capitata.

  2. Producción masiva y simultánea de machos de Ceratitis capitata (Diptera: Tephritidae y parasitoides Dichasmimorpha longicaudata (Hymenoptera: Braconidae Massive and simultaneous production of Ceratitis capitata (Diptera: Tephritidae males and Diachasmimorpha longicaudata (Hymenoptera: Braconidae parasitoids

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    Silvia N. López

    2006-12-01

    Full Text Available En la línea de sexado genético «Cast191» las hembras de Ceratitis capitata son homocigotas para el gen slow , lo que reduce su velocidad de desarrollo; los machos son heterocigotas y muestran una velocidad de desarrollo normal. Esta característica permitió producir, con Cast191, machos estériles por un lado, y parasitoides criados sobre las larvas remanentes por el otro. Nuestro objetivo con este trabajo fue producir ambos insumos simultáneamente y a una escala mayor que hasta ahora. Además, bajo estas condiciones, y en un intento por aumentar la separación entre sexos, se aplicó a las larvas del primer estadío un pulso de 15º C, durante 1 ó 2 días, luego del cual se las mantuvo a 20º C ó 25º C, hasta que entraron al estado de pupa, luego se mantuvo todo el material a 25º C. La mejor separación de sexos, lograda con el tratamiento a 20º C sin pulso de frío, se usó para comparar la calidad del parasitoide Diachasmimorpha longicaudata, criado sobre las larvas obtenidas tras la separación de los machos, con aquellos criados sobre la línea salvaje. Para ello, este tratamiento de separación fue aplicado en la cría de la mosca, y el material remanente de dieta con larvas fue expuesto al parasitoide. La tasa de parasitismo obtenida fue semejante a la hallada sobre la línea salvaje, y la tasa sexual de la F 1 del parasitoide presentó un sesgo hacia las hembras aún mayor. Se discute la factibilidad de utilizar la línea Cast191 de C. Capitata, para la producción a mayor escala de machos de mosca y para la cría masiva del parasitoide D. longicaudata.In the genetic sexing strain «Cast191», the females of Ceratitis capitata are homozygous for the mutation slow , slowing down their rate of development, and the males are heterozygous, having a normal rate of development. This feature made Cast191 capable of producing sterile males, on one hand, and parasitoids that are reared on the remaining larvae, on the other. The

  3. Relación entre tiempo de desarrollo larvario y fecundidad de la hembra en Ceratitis capitata (Diptera:Tephritidae Relationship between time of larval development and fecundity in the female of Ceratitis capitata (Diptera: Tephritidae

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    Fanny C. Manso

    2006-07-01

    Full Text Available Se estudió la relación entre tiempo de desarrollo larvario y fecundidad en la hembra de Ceratitis capitata Wiedemann, comparando individuos de una cepa salvaje, con individuos de una cepa portadora de la mutación sw , la cual provoca atrasos en el desarrollo larvario. Se observaron caracteres de la morfología del ovario y su herencia entre los segregantes, para «tiempo de desarrollo» en la progenie del cruzamiento entre mutante y silvestre. El estudio mostró que las hembras sw (- que quedan muy retrasadas en su desarrollo, tienen menos ovariolas y una distribución de tamaños de óvulos menos homogénea que las que se desarrollan antes. Se encontró asimismo que entre las larvas de tipo salvaje, las más retrasadas también producen hembras con menor número de ovariolas y menos huevos totalmente desarrollados, que las que se desarrollan antes. Tanto en las hembras sw (- como en las sw(+ , los atrasos del desarrollo obran en desmedro de la postura de huevos de las hembras que se desarrollan más tarde, efecto que se manifiesta en el número menor de ovariolas y en las diferencias del ritmo de maduración del ovario. Bajo condiciones apropiadas de cría, las hembras mutantes para sw son capaces de alcanzar la misma fecundidad que las normales.We studied the relationship between the development time in the larva stage and the resulting female fecundity, in Ceratitis capitata Wiedemann wild-type individuals compared to mutant individuals showing slow development (sw. We observed the morphology of the ovaries in the progenies of the crossing between mutant and wild-type strains, comparing females segregating by «development time». This study showed that sw(- females developing later have got less ovarioles, and a more heterogeneous distribution of ovules, than females developing earlier. It was also found that the wild type females developing late also produce lower numbers both of ovarioles and fully developed ova, than those developing

  4. Importance of adult protein ingestion on the mating success of Ceratitis capitata Wiedemann males (Diptera: Tephritidae); Importancia da ingestao de proteina na fase adulta para o sucesso de acasalamento dos machos de Ceratitis capitata Wiedemann (Diptera: Tephritidae)

    Energy Technology Data Exchange (ETDEWEB)

    Silva Neto, Alberto M. da; Dias, Vanessa S.; Joachim-Bravo, Iara S., E-mail: bio.alberto@gmail.co, E-mail: vanessasidias@hotmail.co, E-mail: ibravo@ufba.b [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Inst. de Biologia. Dept. de Biologia Geral

    2010-04-15

    The importance of the protein ingestion during the adult stage on the mating success of males of Ceratitis capitata Wiedemann was evaluated in experiments of laboratory and fi eld cage. In laboratory, the effects of protein ingestion during the fi rst four or 12 days of the male adult life was assessed by the following parameters: mating success (capacity of being chosen by the female) and the number of males that give out pheromonal signals. Some experiments of mating success had been carried through with males in different ratios. In these tests, the number of males which had ingested protein (an unique male) was remained constant and the number of males fed without protein was gradually increased from 1:1 to 1:5. In the fi eld cages, the mating success experiments were done using a 1:1 ratio. The results showed that the protein ingestion in the fi rst four days of life did not influence any of the analyzed parameters. When the period of ingestion of protein was extended to 12 days, protein-fed males fed produced more pheromonal signals and had a higher mating success when at a 1:1 ratio in laboratory and fi eld cage assays. In laboratory, females randomly chose males in any other tested ratio (1:2, 1:3, 1:4 and 1:5), indicating that the female may lose the perception to identify the male who ingested protein in the fi rst 12 days. (author)

  5. Relación entre tiempo de desarrollo larvario y fecundidad de la hembra en Ceratitis capitata (Diptera:Tephritidae

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    Fanny C. MANSO

    2006-01-01

    Full Text Available Se estudió la relación entre tiempo de desarrollo larvario y fecundidad en la hembra de Ceratitis capitata Wiedemann, comparando individuos de una cepa salvaje, con individuos de una cepa portadora de la mutación sw, la cual provoca atrasos en el desarrollo larvario. Se observaron caracteres de la morfología del ovario y su herencia entre los segregantes, para «tiempo de desarrollo» en la progenie del cruzamiento entre mutante y silvestre. El estudio mostró que las hembras sw(- que quedan muy retrasadas en su desarrollo, tienen menos ovariolas y una distribución de tamaños de óvulos menos homogénea que las que se desarrollan antes. Se encontró asimismo que entre las larvas de tipo salvaje, las más retrasadas también producen hembras con menor número de ovariolas y menos huevos totalmente desarrollados, que las que se desarrollan antes. Tanto en las hembras sw(- como en las sw(+, los atrasos del desarrollo obran en desmedro de la postura de huevos de las hembras que se desarrollan más tarde, efecto que se manifiesta en el número menor de ovariolas y en las diferencias del ritmo de maduración del ovario. Bajo condiciones apropiadas de cría, las hembras mutantes para sw son capaces de alcanzar la misma fecundidad que las normales.

  6. Comparison of aggregation and feeding responses by normal and irradiated fruit flies, Ceratitis capitata and Anastrepha suspensa (Diptera: Tephritidae)

    Energy Technology Data Exchange (ETDEWEB)

    Galun, R.; Gothilf, S.; Blondheim, S.; Sharp, J.L.; Mazor, M.; Lachman, A.

    1985-12-01

    Olfactory, aggregatory, and feeding responses of normal (untreated) laboratory stocks of Mediterranean fruit fly (medfly), Ceratitis capitata (Wiedemann), and of Caribbean fruit fly (caribfly), Anastrepha suspensa (Loew), were compared to those of flies irradiated (10 krad in air) 2 days before eclosion. Females of both species consumed greater quantities of protein hydrolysate solutions, entered protein hydrolysate-baited olfactory traps, and aggregated on agar plates containing protein hydrolysate in greater numbers than males of the same age and condition. However, male medflies consumed more sucrose than did females of the same age and condition. In the medfly, irradiation resulted in reduced olfactory response, reduced total food intake by flies of both sexes, and a significant reduction in aggregation on and intake of protein hydrolysate by females and of sugar consumption by males. In the irradiated caribfly, there was a significant reduction in olfactory response of females to yeast hydrolysate. In both sexes, aggregation on and consumption of yeast hydrolysate were reduced. Effects of irradiation on feeding behavior are discussed in relation to the biology of the flies and their control by the sterile insect release method.

  7. Manipulation of the microbiota of mass-reared Mediterranean fruit flies Ceratitis capitata (Diptera: Tephritidae) improves sterile male sexual performance.

    Science.gov (United States)

    Ben Ami, Eyal; Yuval, Boaz; Jurkevitch, Edouard

    2010-01-01

    The sterile insect technique (SIT) is a method of biological control whereby millions of factory reared sterile male insects are released into the field. This technique is commonly used to combat the Mediterranean fruit fly (Ceratitis capitata, Diptera: Tephritidae). Sterile medfly males are less competent in attracting and mating with wild females, a property commonly linked to the irradiation process responsible for the sterilization. As bacteria are important partners in the fly's life cycle, we used molecular analytical methods to study the community structure of the gut microbiota in irradiated male medflies. We find that the sterilizing irradiation procedure affects the gut bacterial community structure of the Mediterranean fruit fly. Although the Enterobacteriaceae family remains the dominant bacterial group present in the gut, the levels of Klebsiella species decreases significantly in the days after sterilization. In addition, we detected substantial differences in some bacterial species between the mass rearing strain Vienna 8 and the wild strain. Most notable among these are the increased levels of the potentially pathogenic species Pseudomonas in the industrial strain. Testing the hypothesis that regenerating the original microbiota community could result in enhanced competitiveness of the sterile flies, we found that the addition of the bacterial species Klebsiella oxytoca to the postirradiation diet enables colonization of these bacteria in the gut while resulting in decreased levels of the Pseudomonas sp. Feeding on diets containing bacteria significantly improved sterile male performance in copulatory tests. Further studies will determine the feasibility of bacterial amelioration in SIT operations.

  8. Impact of the Mediterranean fruit fly (medfly) Ceratitis capitata on different peach cultivars: the possible role of peach volatile compounds.

    Science.gov (United States)

    Tabilio, Maria Rosaria; Fiorini, Dennis; Marcantoni, Enrico; Materazzi, Stefano; Delfini, Maurizio; De Salvador, Flavio Roberto; Musmeci, Sergio

    2013-09-01

    The relationship between susceptibility of different peach cultivars (cvs) to the Mediterranean fruit fly (medfly), Ceratitis capitata, and the volatile composition of ripe fruit of each cv has been investigated, since understanding the fruit-insect interaction mechanism is crucial for developing control strategies for such a pest. Volatile compounds were analyzed by SPME-GC-MS in three cvs highly susceptible to medfly attack (Fair Time, Flaminia, Sicilia Piatta), and in two less susceptible cvs (Percoca Romagnola 7 and Doctor Davis). Among the volatile compounds detected, 88 could be identified. The main differences found in the volatile composition of the cvs, concerned the relative abundance of esters. The least susceptible cvs, above all Percoca Romagnola 7, contained the higher amounts of hexenyl, hexyl, 3-methylbutyl, butyl and 2-methylpropyl esters; among these, some C6 derivatives detected, such as (Z)-3-hexenyl acetate, are known to act as priming agents, enhancing plant defence response to insects. Instead, a lower relative content of methyl esters, such as methyl hexanoate and methyl octanoate, known to act as medfly pheromone and attractant respectively, was found in the least susceptible cvs.

  9. Effects of different protein concentrations on longevity and feeding behavior of two adult populations of Ceratitis capitata Wiedemann (Diptera: Tephritidae)

    Energy Technology Data Exchange (ETDEWEB)

    Placido-Silva, Maria do Carmo; Silva Neto, Alberto M. da; Joachim-Bravo, Iara S. [Universidade Federal da Bahia (UFBA), Salvador, BA (Brazil). Dept. de Biologia Geral; Zucoloto, Fernando S. [Universidade de Sao Paulo (USP), Ribeirao Preto, SP (Brazil). Faculdade de Filosofia Ciencias e Letras. Dept. de Biologia

    2006-11-15

    The effects of protein intake on two adult male and female populations of Ceratitis capitata Wiedemann were assessed. One population consisted of flies reared for twenty years in the laboratory (Lab-pop); the other population consisted both of flies reared in the laboratory for approximately fifteen years and of the periodically introduced wild flies (Hybrid-pop). Three diets were tested: a no-yeast diet and two diets containing yeast (protein source) at the concentrations 6.5 g or 1.5 g per 100 ml diet. The parameters analyzed were: adult longevity, diet intake with and without yeast, and discrimination threshold for yeast. Protein intake increased Lab-pop adult longevity and did not affect longevity of the Hybrid-pop. Longevity in each population was similar for males and females fed on the same diet. Food behavior were similar for male and female adults of both populations; all preferred diets containing protein (yeast). Males and females in both populations ingested similar amounts of each diet. The discrimination threshold for yeast was similar for all males (0.5 g yeast/100 ml diet); Lab-pop females were able to detect the presence of smaller quantities of yeast in their diet, thus having a higher discrimination capacity (0.4 g/100 ml diet) as compared to the Hybrid-pop females (0.6 g/ 100 ml diet). (author)

  10. Insecticidal activity of a Moroccan strain of Streptomyces phaeochromogenes LD-37 on larvae, pupae and adults of the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae).

    Science.gov (United States)

    Samri, S E; Baz, M; Ghalbane, I; El Messoussi, S; Zitouni, A; El Meziane, A; Barakate, M

    2017-04-01

    The Mediterranean fruit fly (medfly), Ceratitis capitata, is considered the most important fruit pest worldwide. Its management is mainly based on the use of chemical insecticides. Although these conventional pesticides are effective at high doses, they cause considerable human health and environment problems. Thus, the aim of this study was to assess insecticidal activity of Moroccan actinobacteria against C. capitata. A total of 12 preselected actinobacteria isolated from various Moroccan habitats were screened for their insecticidal activity against larvae, pupae and adults of C. capitata. Four actinobacteria isolates were significantly active against the first-instar larvae, and nine were active against the medfly adult, while no significant mortality was obtained against the third-instar larval and pupal stages. Among the selected isolates, the biological screening revealed that strain Streptomyces LD-37, which showed 99.4% similarity with Streptomyces phaeochromogenes, exhibited the maximal corrected larval mortality of 98%. Moreover, the isolates AS1 and LD-37 showed the maximum significant corrected mortality against adults of 32.5 and 28.2%, respectively. The crude extract obtained from a fermented culture of strain S. phaeochromogenes LD-37 was separated into six fractions by thin layer chromatography. Fractions F3 and F4 caused a significant corrected larval mortality of 66.7 and 53.3%, respectively; whereas the maximum reduction in adult emergence was obtained with fraction F4. This finding could be useful for utilizing S. phaeochromogenes LD-37 as an alternative to chemical insecticides in pest management of C. capitata.

  11. Temperature-dependent development and survival of Brazilian populations of the Mediterranean fruit fly, Ceratitis capitata, from tropical, subtropical and temperate regions.

    Science.gov (United States)

    Ricalde, Marcelo P; Nava, Dori E; Loeck, Alci E; Donatti, Michele G

    2012-01-01

    The Mediterranean fruit fly Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) is one of the principal exotic pests affecting Brazilian production in the northeastern and southeastern regions of Brazil. In the south, it is has potential as a serious threat to temperate-climate fruit farms, since it is already found in urban and suburban communities in this region. We studied the biological characteristics of C. capitata populations from Pelotas-RS (temperate climate), Petrolina-PE (tropical), and Campinas-SP (subtropical). Ceratitis capitata biology was studied under controlled temperature (15, 20, 25, 30, and 35 ± 1 °C), 70 ± 10% RH, and 14:10 L:D photoperiod. The duration and survival rate of the egg, larval, and pupal stages were evaluated and the thermal requirements of these three populations were determined. The duration and survival of these developmental stages varied with temperature, with similar values for the three populations, except for some variation in the egg phase. Egg to adult developmental time for all three populations was inversely proportional to temperature; from 15 to 30 °C developmental time varied from 71.2 to 17.1, 70.2 to 17.1, and 68.5 to 16.9 days, respectively. Survival during development was affected at 15 to 30 °C, and differed significantly from survival at 20 to 25 °C. At 35 °C, immature stages did not develop. The basal temperature and degree-day requirement were similar for all immature stages except for the egg stage. The basal temperatures and thermal constants were 9.30 and 350, 8.47 and 341, and 9.60 °C and 328 degree-days for the Pelotas, Petrolina, and Campinas populations, respectively. Results suggested that survival and thermal requirements are similar for these tropical, subtropical, and temperate populations of C. capitata, and demonstrate the species' capacity to adapt to different climate conditions.

  12. Effects of within-generation thermal history on the flight performance of Ceratitis capitata: colder is better.

    Science.gov (United States)

    Esterhuizen, Nanike; Clusella-Trullas, Susana; van Daalen, Corne E; Schoombie, Ruben E; Boardman, Leigh; Terblanche, John S

    2014-10-01

    The influence of thermal history on temperature-dependent flight performance was investigated in an invasive agricultural pest insect, Ceratitis capitata (Diptera: Tephritidae). Flies were exposed to one of four developmental acclimation temperatures (Tacc: 15, 20, 25, 30°C) during their pupal stage and tested at these temperatures (Ttest) as adults using a full-factorial study design. Major factors influencing flight performance included sex, body mass, Ttest and the interaction between Ttest and Tacc. Successful flight performance increased with increasing Ttest across all acclimation groups (from 10% at 15°C to 77% at 30°C). Although Tacc did not affect flight performance independently, it did have a significant interaction effect with Ttest. Multiple comparisons showed that flies which had been acclimated to 15°C and 20°C performed better than those acclimated to 25°C and 30°C when tested at cold temperatures, but warm-acclimated flies did not outperform cold-acclimated flies at warmer temperatures. This provides partial support for the 'colder is better' hypothesis. To explain these results, several flight-related traits were examined to determine whether Tacc influenced flight performance as a consequence of changes in body or wing morphology, whole-animal metabolic rate or cytochrome c oxidase enzyme activity. Although significant effects of Tacc could be detected in several of the traits examined, with an emphasis on sex-related differences, increased flight performance could not be explained solely on the basis of changes in any of these traits. Overall, these results are important for understanding dispersal physiology despite the fact that the mechanisms of acclimation-related changes in flight performance remain unresolved.

  13. To Catch a Fly: Landing and Capture of Ceratitis capitata in a Jackson Trap with and without an Insecticide.

    Science.gov (United States)

    Manoukis, Nicholas C

    2016-01-01

    Attractant-based traps are a cornerstone of detection, delimitation and eradication programs for pests such as tephritid fruit flies. The ideal trap and lure combination has high attraction (it brings insects to the trap from a distance) and high capture efficiency (it has a high probability of capturing the insect once it arrives at the trap). We examined the effect of an insecticide (DDVP) in combination with a pheromone lure (trimedlure) on capture of Ceratitis capitata using 1) digital images of surfaces of a Jackson trap analyzed via computer vision, and 2) counts of the number of flies caught in the trap and in the area under the trap. Our results indicate no significant difference in trap capture without or with insecticide (means ± SD = 324 ±135 and 356 ±108, respectively). However, significantly more dead flies were found around the trap with insecticide (92 ±53 with insecticide compared with 35 ±22 without), suggesting a possible decrease in trap efficiency due to mortality before insects enter the trap. Indeed, the average number of flies detected on all surfaces of the traps with insecticide was lower than that for lure-only (4.15±0.39 vs 8.30±1.18), and both were higher than control (no lure: 0.76 ±0.08). We found that the majority of fly sightings, 71% of the total, occurred on the inside panels of the lure-only traps, suggesting that increased efficiency of the Jackson trap may be obtained by adding a contact insecticide to those surfaces.

  14. To Catch a Fly: Landing and Capture of Ceratitis capitata in a Jackson Trap with and without an Insecticide.

    Directory of Open Access Journals (Sweden)

    Nicholas C Manoukis

    Full Text Available Attractant-based traps are a cornerstone of detection, delimitation and eradication programs for pests such as tephritid fruit flies. The ideal trap and lure combination has high attraction (it brings insects to the trap from a distance and high capture efficiency (it has a high probability of capturing the insect once it arrives at the trap. We examined the effect of an insecticide (DDVP in combination with a pheromone lure (trimedlure on capture of Ceratitis capitata using 1 digital images of surfaces of a Jackson trap analyzed via computer vision, and 2 counts of the number of flies caught in the trap and in the area under the trap. Our results indicate no significant difference in trap capture without or with insecticide (means ± SD = 324 ±135 and 356 ±108, respectively. However, significantly more dead flies were found around the trap with insecticide (92 ±53 with insecticide compared with 35 ±22 without, suggesting a possible decrease in trap efficiency due to mortality before insects enter the trap. Indeed, the average number of flies detected on all surfaces of the traps with insecticide was lower than that for lure-only (4.15±0.39 vs 8.30±1.18, and both were higher than control (no lure: 0.76 ±0.08. We found that the majority of fly sightings, 71% of the total, occurred on the inside panels of the lure-only traps, suggesting that increased efficiency of the Jackson trap may be obtained by adding a contact insecticide to those surfaces.

  15. The putative-farnesoic acid O-methyl transferase (FAMeT) gene of Ceratitis capitata: characterization and pre-imaginal life expression.

    Science.gov (United States)

    Vannini, Laura; Ciolfi, Silvia; Spinsanti, Giacomo; Panti, Cristina; Frati, Francesco; Dallai, Romano

    2010-02-01

    Farnesoic acid O-methyl transferase (FAMeT) is the enzyme involved in the penultimate step of insect juvenile hormone (JH) biosynthesis and is thus a key regulator in insect development and reproduction. We report the characterization of the putative-FAMeT in the medfly or Mediterranean fruit fly, Ceratitis capitata. This gene was identified by suppressive subtractive hybridization and completely sequenced by the screening of a medfly cDNA library. The obtained sequence was analyzed for conserved protein domain identification and its expression profile was evaluated by quantitative Real-Time PCR in medfly pre-imaginal life. The tissue expression of the isolated gene was verified by in situ hybridization on third instar larvae sections. The characterization of the isolated gene pointed out several typical features of methyl transferase genes. The pre-imaginal putative-FAMeT expression levels were consistent with JH titer change in Diptera. As recognized in some crustaceans, this gene seems to be widely expressed in the medfly as well. Ceratitis capitata is one of the most relevant agricultural pests against which insecticides and the sterile insect technique (SIT) are extensively used in spite of the well-known limitations of these approaches. Although results are not conclusive for the physiological role of the isolated gene, they suggest the characterization of a new gene in the Mediterranean fruit fly potentially involved in JH biosynthesis and may, therefore, have implications for pest control.

  16. Insecticidal activity of basil oil, trans-anethole, estragole, and linalool to adult fruit flies of Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae.

    Science.gov (United States)

    Chang, Chiou Ling; Cho, Il Kyu; Li, Qing X

    2009-02-01

    Basil oil and its three major active constituents (trans-anethole, estragole, and linalool) obtained from basil (Oscimum basilicum L.) were tested on three tephritid fruit fly species [Ceratitis capitata (Wiedemann), Bactrocera dorsalis (Hendel), and Bactrocera cucurbitae (Coquillett)] for insecticidal activity. All test chemicals acted fast and showed a steep dose-response relationship. The lethal times for 90% mortality/knockdown (LT90) of the three fly species to 10% of the test chemicals were between 8 and 38 min. The toxic action of basil oil in C. capitata occurred significantly faster than in B. cucurbitae but slightly faster than in B. dorsalis. Estragole acted faster in B. dorsalis than in C. capitata and B. cucurbitae. Linalool action was faster in B. dorsalis and C. capitata than in B. cucurbitae. trans-Anethole action was similar to all three species. Methyl eugenol acted faster in C. capitata and B. cucurbitae than in B. dorsalis. When linalool was mixed with cuelure (attractant to B. cucurbitae male), its potency to the three fly species decreased as the concentration of cuelure increased. This was due to linalool hydrolysis catalyzed by acetic acid from cuelure degradation, which was confirmed by chemical analysis. When methyl eugenol (B. dorsalis male attractant) was mixed with basil oil, trans-anethole, estragole, or linalool, it did not affect the toxicity of basil oil and linalool to B. dorsalis, but it did significantly decrease the toxicity of trans-anethole and estragole. Structural similarity between methyl eugenol and trans-anethole and estragole suggests that methyl eugenol might act at a site similar to that of trans-anethole and estragole and serve as an antagonist if an action site exists. Methyl eugenol also may play a physiological role on the toxicity reduction.

  17. Novel RNA viruses producing simultaneous covert infections in Ceratitis capitata. Correlations between viral titers and host fitness, and implications for SIT programs.

    Science.gov (United States)

    Llopis-Giménez, Angel; Maria González, Rosa; Millán-Leiva, Anabel; Catalá, Marta; Llacer, Elena; Urbaneja, Alberto; Herrero, Salvador

    2017-02-01

    The Mediterranean fruit fly (medfly), Ceratitis capitata is a highly polyphagous pest, which infests multiple species of fruits and vegetables worldwide. In addition to the traditional control with chemical insecticides, sterile insect technique (SIT) has been implemented in integrated programs worldwide, and has become an essential measure for the control of this pest. A key issue for SIT is to release sterile males that are sufficiently competitive with males from the wild population. Using sequence information available in public databases, three novel picornaviruses infecting medflies were discovered and named as C. capitata iflavirus 1 and 2 (CcaIV1 and CcaIV2), and C. capitata noravirus (CcaNV). Additional analyses have revealed the presence of CcaIV2 and CcaNV covertly infecting most of the medfly strains used in the different SIT programs around the world, as well as in field captures in the east of Spain. High viral titers of CcaNV were associated with a reduction in the lifespan of males released to the field for the control of this pest, suggesting the possibility that CcaNV may impair the fitness of sterile flies produced by SIT programs.

  18. Eggs of Ephestia kuehniella and Ceratitis capitata, and motile stages of the astigmatid mites Tyrophagus putrescentiae and Carpoglyphus lactis as factitious foods for Orius spp.

    Science.gov (United States)

    Bonte, Jochem; de Walle, Anaïs Van; Conlong, Des; De Clercq, Patrick

    2015-11-17

    Several factitious foods were assessed for rearing the anthocorid predators Orius thripoborus (Hesse) and Orius naivashae (Poppius) (Hemiptera: Anthocoridae) in the laboratory. Developmental and reproductive traits of both Orius species were examined when offered frozen eggs of the Mediterranean flour moth, Ephestia kuehniella Zeller, frozen processed eggs of the medfly, Ceratitis capitata Wiedemann, or mixed motile stages of the astigmatid mites Tyrophagus putrescentiae (Schrank) or Carpoglyphus lactis (L). Whereas C. lactis and T. putresecentiae proved to be an inferior food for rearing O. thripoborus and O. naivashae, eggs of C. capitata eggs fully supported development and reproduction of both predators. Results on medfly eggs were similar or slightly inferior to those on E. kuehniella eggs, which is the standard food for culturing these anthocorid bugs. O. thripoborus could be maintained for four consecutive generations on C. capitata eggs indicating that processed medfly eggs can be a suitable and cheaper alternative to E. kuehniella eggs for prolonged rearing of these Orius spp. This article is protected by copyright. All rights reserved.

  19. Captures of Wild Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae (Diptera: Tephritidae) in Traps with Improved Multilure TMR Dispensers Weathered in California.

    Science.gov (United States)

    Vargas, Roger I; Souder, Steven K; Morse, Joseph G; Grafton-Cardwell, Elizabeth E; Haviland, David R; Kabashima, John N; Faber, Ben A; Mackey, Bruce; Cook, Peter

    2016-04-01

    During 2012–2013, solid Mallet TMR (trimedlure [TML], methyl eugenol [ME], raspberry ketone [RK]) wafers impregnated with DDVP (2, 2-dichlorovinyl dimethyl phosphate) insecticide were weathered during summer (8 wk) and winter (12 wk) in five California citrus-growing counties (Kern, Ventura, Orange, Tulare, and Riverside). In addition, TMR wafers without DDVP and with a Hercon Vaportape II insecticidal strip were compared with TMR dispensers with DDVP at Exeter and Riverside. Weathered treatments were shipped every week (overnight delivery) to Hawaii and frozen for a later bioassay in a 1,335-ha coffee plantation near Numila, Kauai Island, HI, where Mediterranean fruit fly, Ceratitis capitata (Wiedemann), oriental fruit fly, Bactrocera dorsalis Hendel, and melon fly, Bactrocera cucurbitae Coquillett, were all present. We compared trap captures of the three species, C. capitata, B. dorsalis, and B. cucurbitae, for the five different weathering locations. Captures of C. capitata, B. dorsalis, and B. cucurbitae with Mallet TMR dispensers (with DDVP) were not significantly different for the five locations. Captures with the Mallet TMR dispenser without DDVP and Vaportape were similar to those for Mallet TMR with DDVP, although there were some slight location differences. In conclusion, based on these results, the Mallet TMR dispenser could potentially be used in California habitats where large numbers of detection traps are currently deployed. Use of Vaportape with dispensers would not require them to be registered with US Environmental Protection Agency (EPA). Dispensers for use as Male Annihilation Technique (MAT) devices will be tested further in Hawaii.

  20. Composition and anti-insect activity of essential oils from Tagetes L. species (Asteraceae, Helenieae) on Ceratitis capitata Wiedemann and Triatoma infestans Klug.

    Science.gov (United States)

    López, Sandra B; López, María L; Aragón, Liliana M; Tereschuk, María L; Slanis, Alberto C; Feresin, Gabriela E; Zygadlo, Julio A; Tapia, Alejandro A

    2011-05-25

    Essential oils from four species of the genus Tagetes L. (Asteraceae, Helenieae) collected in Tucumán province, Argentina, were evaluated for their chemical composition, toxicity, and olfactory activity on Mediterranean fruit fly Ceratitis capitata Wiedemann adults and for repellent properties on Triatoma infestans (Klug) (Chagas disease vector). Yields of essential oils range from 0.2 to 0.8% (v/w). The same main constituents among Tagetes minuta L., Tagestes rupestris Cabrera, and Tagetes terniflora Kunth, (cis-trans)-ocimenes, (cis-trans)-tagetones, and (cis-trans)-ocimenones showed important differences in their relative compositions. Tagetes filifolia Lag. was characterized by the recognized phenylpropanoids methylchavicol and trans-anethole as the main components. LD(50) was ≤20 μg/insect in topical bioassays. T. rupestris was the most toxic to C. capitata females, whereas the other oils presented similar toxicities against males and females. Tagetes rupestris oil attracted both sexes of C. capitata at 5 μg, whereas T. minuta showed opposite activities between males (attractant) and females (repellent). Oils from T. minuta and T. filifolia were the most repellent to T. infestans. The results suggest that compositions of essential oils influence their insecticidal and olfactory properties. The essential oils from Tagetes species show an important potential as infochemical agents on insects' behaviors. This study highlights the chemical variability of essential oils as a source of variation of anti-insect properties.

  1. Area-wide suppression of the Mediterranean fruit fly, Ceratitis capitata, and the Oriental fruit fly, Bactrocera dorsalis, in Kamuela, Hawaii.

    Science.gov (United States)

    Vargas, Roger I; Piñero, Jaime C; Mau, Ronald F L; Jang, Eric B; Klungness, Lester M; McInnis, Donald O; Harris, Ernest B; McQuate, Grant T; Bautista, Renato C; Wong, Lyle

    2010-01-01

    The United States Department of Agriculture's Agricultural Research Service initiated an area-wide fruit fly management program in Hawaii in 2000. The first demonstration site was established in Kamuela, Hawaii, USA. This paper documents suppression of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), and the oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae), in a 40 km2 area containing urban, rural and agricultural zones during a 6 year period. The suppression techniques included sanitation, GF-120 NF Naturalyte Fruit Fly Bait sprays, male annihilation, Biolure traps, and parasitoids against C. capitata and B. dorsalis. In addition, small numbers of sterile males were released against B. dorsalis. Substantial reductions in fruit infestation levels were achieved for both species (90.7 and 60.7% for C. capitata and B. dorsalis, respectively) throughout the treatment period. Fruit fly captures in the 40 km2 treatment area were significantly lower during the 6 year period than those recorded in three non-treated areas. The strategy of combining suppression techniques in an area-wide approach is discussed.

  2. Response of female Ceratitis capitata (Diptera: Tephritidae) to a spinosad bait and polymer matrix mixture with extended residual effect in Hawaii.

    Science.gov (United States)

    Piñero, Jaime C; Souder, Steven K; Gomez, Luis E; Mau, Ronald F L; Vargas, Roger I

    2011-12-01

    The effectiveness of foliar applications of protein baits against pestiferous fruit flies (Tephritidae) can be adversely affected by a rapid loss of attractive volatile compounds and by rainfall due to the high water solubility of the baits. In a large coffee, Coffea arabica L., plantation in Hawaii with high and low populations of Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), the relative attractiveness of GF-120 NF Naturalyte Fruit Fly Bait as either a 40% (vol:vol) spray solution (= GF-120 NF) or as a formulated proprietary amorphous polymer matrix (= GF-120 APM) was compared. The GF-120 APM formulations contained either, 25, 50, or 75% of GF-120 NF (wt:wt). All baits were tested in association with visually attractive yellow bait stations as a way of standardizing the evaluations. With both high and low C. capitata populations, significantly more females were attracted to the fresh sprayed GF-120 NF than to any of the three fresh GF-120 APM formulations. The attractiveness of GF-120 sprayed decreased significantly after 1 wk, whereas 1-wk-old GF-120 APM formulations were as attractive as similar fresh formulations. GF-120 APM 75% aged for 3 wk outperformed similarly-aged sprayed GF-120 NF with comparatively high C. capitata populations. With low populations, both GF-120 APM 75% and GF-120 APM 50% aged for 2 wk outperformed the similarly aged sprayed GF-120 NF. Combined findings indicate that APM mixed with either 50 or 75% GF-120 applied to bait stations can be attractive to female C. capitata for up to 3 wk longer than the standard sprayed GF-120 NF.

  3. Constitutive expression and enzymatic activity of Tan protein in brain and epidermis of Ceratitis capitata and of Drosophila melanogaster wild-type and tan mutants.

    Science.gov (United States)

    Pérez, M M; Sabio, G; Badaracco, A; Quesada-Allué, L A

    2011-09-01

    The present report shows a partial biochemical characterization and life cycle expression of N-β-alanyldopamine hydrolase (Tan protein) in Ceratitis capitata and Drosophila melanogaster. This enzyme catalyzes the hydrolysis of N-β-alanyldopamine (NBAD), the main tanning precursor of insect brown cuticles. It also plays an important role in the metabolism of brain neurotransmitters, recycling dopamine and histamine. In contrast to NBAD-synthase, Tan is expressed constitutively in epidermis and does not respond directly to microbial challenge. Immunodetection experiments showed the novel localization of NBAD-hydrolase in the embryo central neural system and in different regions of the adult brain, in addition to optic lobes. We sequenced and characterized Drosophila mutants tan¹ and tan³. The latter appears to be a mutant with normal expression in neural tissue but weak one in epidermis.

  4. Introducción y producción en laboratorio de Diachasmimorpha tryon i y Diachasmimorpha longicaudata (Hymenoptera: Braconidae para el control biológico de Ceratitis capitata (Diptera: Tephritidae en la Argentina Introduction and laboratory production of Diachasmimorpha tryoni and Diachasmimorpha longicaudata (Hymenoptera: Braconidae for the biological control of Ceratitis capitata (Diptera: Tephritidae in Argentina

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    Sergio M. Ovruski

    2003-12-01

    Full Text Available Con el propósito de reanudar la utilización de enemigos naturales contra la especie exótica Ceratitis capitata (Wiedemann, fueron introducidos a la Argentina en 1999 los agentes de control biológico Diachasmimorpha tryoni (Cameron y Diachasmimorpha longicaudata (Ashmead, dos endoparasitoides de larvas de tefrítidos. Por este motivo, en este trabajo se describen los procedimientos de cría en laboratorio del huésped y de ambas especies de parasitoides y, se presentan y discuten los resultados de un año de producción de D. tryoni y D. longicaudata a mediana escala (enero-diciembre/2000. Se realizó un análisis comparativo de los datos obtenidos sobre la producción de descendientes, proporción sexual, porcentaje de parasitismo y viabilidad de puparios por jaula de cría durante 15 generaciones entre ambas especies de parasitoides exóticos, utilizando como huésped larvas de C. capitata del tercer estadio de siete días de edad. Además, se discuten las posibilidades para implementar el control biológico aumentativo contra C. capitata y Anastrepha fraterculus (Wiedemann en el país.The biocontrol agents Diachasmimorpha tryoni (Cameron and Diachasmimorpha longicaudata (Ashmead, two endoparasitoids of fruit fly larvae, were introduced to Argentina in 1999 with the purpose of renewing the employment of natural enemies against Ceratitis capitata (Wiedemann. For this reason, the general procedure and maintenance of the host and parasitoids rearing in the laboratory are described, and the results of one year insectary production (January-December/2000 of both D. tryoni and D. longicaudata are discussed. Data are presented of the progeny production, offpring sex ratio, host parasitism percentage, and pupal viability per parasitoid rearing cage during 15 generations of D. longicaudata and D. tryoni reared using late third instar larvae of C. capitata. New perspectives are discussed on the establishment of a biological control program for C

  5. Ceratitis capitata transformer-2 gene is required to establish and maintain the autoregulation of Cctra, the master gene for female sex determination.

    Science.gov (United States)

    Salvemini, Marco; Robertson, Mark; Aronson, Benjamin; Atkinson, Peter; Polito, Lino C; Saccone, Giuseppe

    2009-01-01

    In Drosophila melanogaster, transformer-2 (TRA-2) which is a non-sex-specific auxiliary splicing factor, is required to promote female sexual differentiation by interaction with the female-specific TRA. The two proteins positively regulate the splicing of both doublesex (dsx) and fruitless (fru) pre-mRNAs, which in turn regulate phenotypic and behavioural sexual dimorphism. In the Mediterranean fruitfly Ceratitis capitata, the female-specific CcTRA is similarly required not only for Ccdsx splicing, but also to exert a novel autoregulatory function that consists of promoting female-specific splicing of Cctra pre-mRNA. This study reports the isolation and functional analysis of the C. capitata homologue of the Drosophila transformer-2 gene (Cctra-2). Transient RNAi against Cctra-2 during embryonic development causes the full sex reversal of XX flies in adult fertile pseudo-males, as well as changes in the splicing pattern of Cctra, Ccdsx and Ccfruitless (Ccfru). We propose that: 1) Cctra-2, as in Drosophila, is necessary for promoting Ccdsx and putative Ccfru pre-mRNA female-specific splicing and that 2) unlike in Drosophila, Cctra-2 appears to be necessary for establishing female sex determination in early XX embryos and for maintaining the positive feedback regulation of Cctra during development.

  6. Quarantine cold treatments for Ceratitis capitata and Anastrepha fraterculus (Diptera: Tephritidae) for citrus in Argentina: conclusions after 10 years of research

    Energy Technology Data Exchange (ETDEWEB)

    Willink, Eduardo; Gastaminza, Gerardo; Salvatore, Analia; Gramajo, M. Cecilia; Acenolaza, Mariana; Avila, Rosana; Favre, Paola, E-mail: ewillink@eeaoc.org.a [Estacion Experimental Agroindustrial Obispo Colombres (EEAOC), Tucuman (Argentina)

    2006-07-01

    Argentina has quarantine restrictions in some markets due to the presence of two quarantine fruit fly pests: Ceratitis capitata and Anastrepha fraterculus. One alternative is the use of cold quarantine treatments during transport of the commodities. Since 1996, the Estacion Experimental Agroindustrial Obispo Colombres (EEAOC), Tucuman, Argentina, has developed different cold quarantine treatments for citrus. In the present work we present all the data the EEAOC generated in the last ten years in order to facilitate the development of such cold treatments. Fruit flies were obtained from the colonies reared at EEAOC. Four citrus species were analyzed: lemon, grapefruit, orange and tangerines. Different varieties were analyzed for each fruit species. Sensitivity trials aiming at determine the most tolerant stage as well as to asses if there is any influence of varieties on cold tolerance were performed. Finally we compared the tolerance to cold between the two species. Sensitivity trials showed that mature larvae (L3) are the most tolerant stage for both fruit fly species. There was no effect of the varieties and the two fruit fly species were equally sensible to cold. Our results provide strong evidence in favor of concluding that any cold treatment developed for C. capitata is effective for A. fraterculus. (author)

  7. Analysis of survival, gene expression and behavior following chill-coma in the medfly Ceratitis capitata: effects of population heterogeneity and age.

    Science.gov (United States)

    Pujol-Lereis, Luciana Mercedes; Rabossi, Alejandro; Quesada-Allué, Luis Alberto

    2014-12-01

    The medfly Ceratitis capitata is an agricultural pest distributed worldwide thanks, in part, to its phenotypic plasticity of thermal tolerance. Cold exposure has been shown to reduce C. capitata survival, which may affect its distribution in areas with subfreezing temperatures. When insects are increasingly cooled, they attain a critical thermal threshold and enter a chill-coma state characterized by cessation of movement. It is not clear how a rapid cold exposure affects the physiological state of medflies, and how this is influenced by age and population heterogeneity. In order to approach these questions, C. capitata single-sex laboratory populations of 15 and 30 days old were subjected to a chill-coma recovery assay, and separated according to their recovery time in three subgroups: Fast-Subgroups, Intermediate-Subgroups, and Slow-Subgroups. Thereafter, we analyzed their survival, behavioral, and gene expression outputs. In female and old male populations, we found that flies with the slowest recovery time had a reduced life expectancy, a higher initial mortality rate, and a worse climbing performance compared with flies that recovered faster. Therefore, we were able to separate subgroups that developed chilling-injury from subgroups that had a reversible full recovery after cold exposure. The gene expression analysis of the heat shock protein genes hsp70 and hsp83 showed no clear association with the parameters studied. Interestingly, thorax expression levels of the Cu/Zn superoxide dismutase gene were elevated during the recovery phase in the Fast-Subgroups, but remained constant in the Slow-Subgroups that developed chilling-injury. On the other hand, none of the young male subgroups seemed to have suffered irreversible damage. Thus, we concluded that depending on age and population heterogeneity, chill-coma recovery time points out significant differences on individual cold tolerance. Moreover, the inability to properly induce the antioxidant defense system

  8. Identification of pheromone components and their binding affinity to the odorant binding protein CcapOBP83a-2 of the Mediterranean fruit fly, Ceratitis capitata.

    Science.gov (United States)

    Siciliano, P; He, X L; Woodcock, C; Pickett, J A; Field, L M; Birkett, M A; Kalinova, B; Gomulski, L M; Scolari, F; Gasperi, G; Malacrida, A R; Zhou, J J

    2014-05-01

    The Mediterranean fruit fly (or medfly), Ceratitis capitata (Wiedemann; Diptera: Tephritidae), is a serious pest of agriculture worldwide, displaying a very wide larval host range with more than 250 different species of fruit and vegetables. Olfaction plays a key role in the invasive potential of this species. Unfortunately, the pheromone communication system of the medfly is complex and still not well established. In this study, we report the isolation of chemicals emitted by sexually mature individuals during the "calling" period and the electrophysiological responses that these compounds elicit on the antennae of male and female flies. Fifteen compounds with electrophysiological activity were isolated and identified in male emissions by gas chromatography coupled to electroantennography (GC-EAG). Within the group of 15 identified compounds, 11 elicited a response in antennae of both sexes, whilst 4 elicited a response only in female antennae. The binding affinity of these compounds, plus 4 additional compounds known to be behaviourally active from other studies, was measured using C. capitata OBP, CcapOBP83a-2. This OBP has a high homology to Drosophila melanogaster OBPs OS-E and OS-F, which are associated with trichoid sensilla and co-expressed with the well-studied Drosophila pheromone binding protein LUSH. The results provide evidence of involvement of CcapOBP83a-2 in the medfly's odorant perception and its wider specificity for (E,E)-α-farnesene, one of the five major compounds in medfly male pheromone emission. This represents the first step in the clarification of the C. capitata and pheromone reception pathway, and a starting point for further studies aimed towards the creation of new powerful attractants or repellents applicable in the actual control strategies.

  9. Subtractive and differential hybridization molecular analyses of Ceratitis capitata XX/XY versus XX embryos to search for male-specific early transcribed genes.

    Science.gov (United States)

    Salvemini, Marco; D'Amato, Rocco; Petrella, Valeria; Ippolito, Domenica; Ventre, Giuseppe; Zhang, Ying; Saccone, Giuseppe

    2014-01-01

    The agricultural pest Ceratitis capitata, also known as the Mediterranean fruit fly or Medfly, is a fruit crop pest of very high economic relevance in different continents. The strategy to separate Ceratitis males from females (sexing) in mass rearing facilities is a useful step before the sterilization and release of male-only flies in Sterile Insect Technique control programs (SIT). The identification of genes having early embryonic male-specific expression, including Y-linked genes, such as the Maleness factor, could help to design novel and improved methods of sexing in combination with transgenesis, aiming to confer conditional female-specific lethality or female-to-male sexual reversal. We used a combination of Suppression Subtractive Hybrydization (SSH), Mirror Orientation Selection (MOS) anddifferential screening hybridization (DSH) techniques to approach the problem of isolating corresponding mRNAs expressed in XX/XY embryos versus XX-only embryos during a narrow developmental window (8-10 hours after egg laying, AEL ). Here we describe a novel strategy we have conceived to obtain relatively large amounts of XX-only embryos staged at 8-10 h AEL and so to extract few micrograms of polyA+ required to apply the complex technical procedure. The combination of these 3 techniques led to the identification of a Y-linked putative gene, CcGm2, sharing high sequence identity to a paralogous gene, CcGm1, localized either on an autosome or on the X chromosome. We propose that CcGm2 is a first interesting putative Y-linked gene which could play a role in sex determination. The function exterted by this gene should be investigated by novel genetic tools, such as CRISPR-CAS9, which will permit to target only the Y-linked paralogue, avoiding to interfere with the autosomal or X-linked paralogue function.

  10. MEDHOST: An encyclopedic bibliography of the host plants of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), version 1.1

    Science.gov (United States)

    This monograph is a compendium of all plant species reported as hosts of C. capitata (or potential hosts based on mere appearance on some lists). There are 353 species (312 have valid genera and species names and 41 have species identified as "sp." or "spp.") included in the monograph; 79 species ha...

  11. Effects of pesticides used on citrus grown in Spain on the mortality of Ceratitis capitata (Diptera: Tephritidae) Vienna-8 strain sterile males.

    Science.gov (United States)

    Juan-Blasco, María; Sabatier-Muñoz, Beatriz; Argilés, Rafael; Jacas, Josep A; Ortego, Félix; Urbaneja, Alberto

    2013-06-01

    Vienna-8 sterile males are currently released in Spain to reduce wild populations of the medfly. Because pesticides are required to maintain populations of some citrus key pests below economic thresholds, there is a need to evaluate the effects of pesticides commonly used in citrus on Vienna-8 males. Males were exposed to differently aged residues of eight pesticides. Abamectin, etofenprox, etoxazole, petroleum spray oil, pymetrozine, and pyriproxyfen resulted harmless to Vienna-8 males. However, fresh residues of chlorpyrifos and spinosad caused high mortalities and had residual effects until 21 and 28 d after treatment, respectively. Following the same method, the lethal effects of chlorpyrifos and spinosad on Ceratitis capitata (Wiedemann) wild-type (wt) males were determined. Surprisingly, these pesticides resulted more toxic for wt than for Vienna-8 males. To determine whether these results could be attributed to intrinsic characteristics of the pesticides or to behavioral differences among Vienna-8 and wt males, a topical application trial was conducted. Vienna-8 males were twice as susceptible to chlorpyrifos as wt males, whereas their susceptibility to spinosad was slightly lower. These results in intrinsic toxicity did not directly explain the differences observed in the extended-laboratory tests with these pesticides. We hypothesize that the lower flight activity ofVienna-8 males relative to wt conspecifics can explain the lower risk observed for Vienna-8 males in the residual tests. Our results should be taken into account when planning area-wide Sterile Insect Technique programs against C. capitata especially in those areas where treatments with chlorpyrifos are approved.

  12. Tratamento hidrotérmico na mortalidade de larvas de Ceratitis capitata (Weidmann, 1824 (Diptera: Tephritidae em tangerina (Citrus reticulata Blanco = Effect of hydrothermal treatment on the mortality of fruit fly (Ceratitis capitata Weidmann, 1824 (Diptera: Tephritidae larvae in tangerine fruit (Citrus reticulata Blanco

    Directory of Open Access Journals (Sweden)

    Edson Batista Lopes

    2008-12-01

    Full Text Available O objetivo dessa pesquisa foi avaliar a eficiência do tratamento hidrotérmico no controle de larvas de C. capitata em frutos de tangerina para fins quarentenários. Tangerinas da variedade ‘Dancy’ infestadas por larvas de Ceratitis capitata foram submetidas aostratamentos hidrotérmicos de 46±1°C e 50±1°C, em diferentes tempos de exposição. Para a temperatura de 46±1°C foram utilizados nove períodos de exposição: 0, 5, 10, 15, 20, 25, 30, 35 e 40 min. Na temperatura de 50±1°C, utilizaram-se sete tempos de exposição: 0, 5,10, 15, 20, 25 e 30 min., sendo o tempo (0 infestado e sem tratamento para as duas temperaturas. Cada tratamento foi composto de cinco repetições com dois frutos. Frutos maduros e semi-maduros infestados foram imersos em água quente, visando quantificar ototal de larvas vivas e mortas, sendo que, um grupo de frutos foi mantido sem imersão como controle. Os índices quarentenários, na temperatura de 46±1°C foram atingidos aos 32,0 e 35,3 min. para frutos maduros e semi-maduros, respectivamente. Na temperatura de50±1°C, os índices quarentenários foram atingidos aos 20,8 e 21,7 minutos para os frutos maduros e semi-maduros, respectivamente. A mortalidade das larvas de C. capitata foi crescente com o aumento da temperatura e o tempo de exposição.This research aimed to evaluate the efficiency of hydrothermaltreatment in the control of C. capitata larvae in tangerine fruit for quarantine safety. Tangerines fruits infested by larvae of Mediterranean fruit fly Ceratitis capitata were submitted to hydrothermal treatment at 46±1°C and 50±1°C for different exposure times. The fruits were selected in two maturity stages (ripe and semi-ripe and immersed in hot water. For temperature at 46±1°C, nine exposure times were used: 0, 5, 10, 15, 20, 25, 30, 35 and 40 minutes. The same procedure was used for temperature at 50±1°C, where the exposure times were: 0, 5, 10, 15, 20, 25 and 30 minutes. Infested

  13. Variation in adult sex ratio alters the association between courtship, mating frequency and paternity in the lek-forming fruitfly Ceratitis capitata.

    Science.gov (United States)

    Leftwich, P T; Edward, D A; Alphey, L; Gage, M J G; Chapman, T

    2012-09-01

    The intensity with which males deliver courtship and the frequency with which they mate are key components of male reproductive success. However, we expect the strength of the relationship between these traits and a male's overall paternity to be strongly context dependent, for example to be altered significantly by the extent of post-mating competition. We tested this prediction in a lekking insect, Ceratitis capitata (medfly). We examined the effect of manipulating the sex ratio from male- to female-biased (high and low male competition, respectively) on courtship behaviour, mating frequency and paternity of focal males. Under high male competition, focal males delivered significantly more courtship but gained lower paternity than under lower competition. Paternity was positively associated with mating frequency and small residual testes size. However, the association between mating frequency and paternity was significantly stronger under low competition. We conclude that manipulation of sex ratio significantly altered the predictors of mating success and paternity. The relationship between pre- and post-mating success is therefore plastic and alters according to the prevailing level of competition. The results highlight the importance of post-copulatory processes in lekking species and illuminate selection pressures placed on insects such as medflies that are mass reared for pest control.

  14. Hydrogen peroxide is produced by E. coli challenged haemocytes and regulates phagocytosis, in the medfly Ceratitis capitata. The active role of superoxide dismutase.

    Science.gov (United States)

    Arbi, Marina; Pouliliou, Stamatia; Lampropoulou, Maria; Marmaras, Vassilis J; Tsakas, Sotiris

    2011-08-01

    Hydrogen peroxide (H(2)O(2)) participates as a second messenger in cell signaling. In this paper, the role of H(2)O(2) was investigated, in Escherichia coli phagocytosis by the haemocytes of the medfly Ceratitis capitata. Block of H(2)O(2) synthesis by specific enzymic inhibitors, namely N-ethylmaleimide (NEM) for NADPH oxidase and diethyldithiocarbamate (DDC) for SOD, resulted in the increase of E. coli phagocytosis. Immunoblot analysis, flow cytometry and confocal microscopy, revealed the constitutive expression of SOD, in the medfly haemocytes. Phagocytosis increased by small interfering RNA (siRNA) for SOD, revealing the active involvement of SOD and H(2)O(2). Immunoblot analysis showed an increase of the ERK1/2 phosphorylation, in the presence of the above H(2)O(2) synthesis enzymic inhibitors. In addition, confocal microscopy showed no co-localization of SOD with β integrin subunit. It appears that SOD participates in the regulation of bacterial phagocytosis, due to involvement of the produced H(2)O(2) in the differential phosphorylation of MAP kinases.

  15. Fitness improvement of mass-reared sterile males of Ceratitis capitata (Vienna 8 strain) (Diptera: Tephritidae) after gut enrichment with probiotics.

    Science.gov (United States)

    Hamden, Heithem; Guerfali, Meriem M'Saad; Fadhl, Selma; Saidi, Mouldi; Chevrier, Claude

    2013-04-01

    Successful mass rearing is crucial for sterile insect technique programs. It has been shown that the sterilizing process using gammaradiation results in damage to midgut tissue, cellular organelles, and gut microbiota of flies. This can be responsible for the inferiority of sterile males compared with wild males. A bacteria-enhanced diet could contribute to the improvement of the fly's fitness. We investigated ways of increasing the competitiveness of mass-reared Mediterranean fruit fly, Ceratitis capitata (Wiedemann) sterile males. We tested the hypothesis that the addition of beneficial bacteria to the larvae's diet would lead to a significant increase in their levels in the gut of the sterile adults and consequently improve their size and fitness. As expected, enriching the diet of mass-rearing Vienna-8 strain larvae with beneficial bacteria (Klebsiella pneumonia, Enterobacter spp., and Citrobacter freundii) resulted in increase in the number of Enterobacteriacae communities inhabiting the male's gut and a subsequent significant increase in the size of males and other morphometric traits and enhanced sexual performance of males at emergence.

  16. The role of protein in the sexual behaviour of males of Ceratitis capitata (Diptera: Tephritidae: mating success, copula duration and number of copulations

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    Iara Sordi Joachim-Bravo

    2009-09-01

    Full Text Available In this paper, we investigated the influence of protein ingestion during the early adult phase on the sexual behavior of males of Ceratitis capitata (Wiedemann, 1824. The following parameters were evaluated: mating success (ability to be chosen by females, number of copulations, and copula duration. Experiments were carried out using a fifteen-year old laboratory lineage with the occasional introduction of wild flies. Two groups of adult males fed a high-protein diet during their larval phase were given either a high-protein diet (based on Brewer's yeast, concentration = 6.5 g/100 ml or a no-protein diet. Both groups of males were exposed to females fed either a high-protein diet or a no-protein diet and were subsequently evaluated for the parameters listed above. All experiments were conducted at 25ºC, 70% R.H. and with a photoperiod of L12:D12. The number of copulations was the only parameter affected by adult diet. Males fed a high-protein diet and exposed to females fed a no-protein diet had a greater number of copulations compared with males fed a no-protein diet.

  17. A centralised remote data collection system using automated traps for managing and controlling the population of the Mediterranean (Ceratitis capitata) and olive (Dacus oleae) fruit flies

    Science.gov (United States)

    Philimis, Panayiotis; Psimolophitis, Elias; Hadjiyiannis, Stavros; Giusti, Alessandro; Perelló, Josep; Serrat, Albert; Avila, Pedro

    2013-08-01

    The present paper describes the development of a novel monitoring system (e-FlyWatch system) for managing and controlling the population of two of the world's most destructive fruit pests, namely the olive fruit fly (Bactrocera oleae, Rossi - formerly Dacus oleae) and the Mediterranean fruit fly (Ceratitis capitata, also called medfly). The novel monitoring system consists of a) novel automated traps with optical and motion detection modules for capturing the flies, b) local stations including a GSM/GPRS module, sensors, flash memory, battery, antenna etc. and c) a central station that collects, stores and publishes the results (i.e. insect population in each field, sensor data, possible error/alarm data) via a web-based management software.The centralised data collection system provides also analysis and prediction models, end-user warning modules and historical analysis of infested areas. The e-FlyWatch system enables the SMEs-producers in the Fruit, Vegetable and Olive sectors to improve their production reduce the amount of insecticides/pesticides used and consequently the labour cost for spraying activities, and the labour cost for traps inspection.

  18. Hidrogenionic potential (pH of the attractant, trap density and control threshold for Ceratitis capitata (DIPTERA: TEPHRITIDAE on Hamlin oranges in São Paulo central region, Brazil

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    Paulo Eduardo Branco Paiva

    2013-06-01

    Full Text Available This study evaluated the effect of initial pH values of 4.5, 6.5 and 8.5 of the attractant (protein bait Milhocina® and borax (sodium borate in the field, on the capture of fruit flies in McPhail traps, using 1, 2, 4 and 8 traps per hectare, in order to estimate control thresholds in a Hamlin orange grove in the central region of the state of São Paulo. The most abundant fruit fly species was Ceratitis capitata, comprising almost 99% of the fruit flies captured, of which 80% were females. The largest captures of C. capitata were found in traps baited with Milhocina® and borax at pH 8.5. Captures per trap for the four densities were similar, indicating that the population can be estimated with one trap per hectare in areas with high populations. It was found positive relationships between captures of C. capitata and the number of Hamlin oranges damaged, 2 and 3 weeks after capture. It was obtained equations that correlate captures and damage levels which can be used to estimate control thresholds. The average loss caused in Hamlin orange fruits by C. capitata was 2.5 tons per hectare or 7.5% of production.

  19. Producción masiva y simultánea de machos de Ceratitis capitata (Diptera: Tephritidae y parasitoides Dichasmimorpha longicaudata (Hymenoptera: Braconidae

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    Silvia N. LÓPEZ

    2006-01-01

    Full Text Available En la línea de sexado genético «Cast191» las hembras de Ceratitis capitata son homocigotas para el gen slow , lo que reduce su velocidad de desarrollo; los machos son heterocigotas y muestran una velocidad de desarrollo normal. Esta característica permitió producir, con Cast191, machos estériles por un lado, y parasitoides criados sobre las larvas remanentes por el otro. Nuestro objetivo con este trabajo fue producir ambos insumos simultáneamente y a una escala mayor que hasta ahora. Además, bajo estas condiciones, y en un intento por aumentar la separación entre sexos, se aplicó a las larvas del primer estadío un pulso de 15º C, durante 1 ó 2 días, luego del cual se las mantuvo a 20º C ó 25º C, hasta que entraron al estado de pupa, luego se mantuvo todo el material a 25º C. La mejor separación de sexos, lograda con el tratamiento a 20º C sin pulso de frío, se usó para comparar la calidad del parasitoide Diachasmimorpha longicaudata, criado sobre las larvas obtenidas tras la separación de los machos, con aquellos criados sobre la línea salvaje. Para ello, este tratamiento de separación fue aplicado en la cría de la mosca, y el material remanente de dieta con larvas fue expuesto al parasitoide. La tasa de parasitismo obtenida fue semejante a la hallada sobre la línea salvaje, y la tasa sexual de la F1 del parasitoide presentó un sesgo hacia las hembras aún mayor. Se discute la factibilidad de utilizar la línea Cast191 de C. Capitata, para la producción a mayor escala de machos de mosca y para la cría masiva del parasitoide D. longicaudata.

  20. Comportamento Reprodutivo de Ceratitis capitata Wiedemann (Diptera: Tephritidae: Efeito do Tamanho dos Machos Sobre o Seu Sucesso de Cópula.

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    Alberto Silva Neto

    2012-11-01

    Abstract. This work evaluated the influence of size on the copula success in Ceratitis capitata (Wiedemann. To assure the production of different adult sizes (big and small, two groups of larvae had been fed with different protein concentrations. Subsequently, adult males of both groups had been compared in terms of copula success and amount of males who showed the first step of courtship (emission of sexual pheromone. The copula success in laboratory was evaluated with males in some ratios, which the number of big males with five days of life (an unique male in relation to a gradual increase of small males with same age kept constant. The tested ratios had been 1:1, 1:2, 1:3, 1:4, 1:5, and 1:10. In the experiments of copula success in the 1:1 ratio and the ones of pheromone emission, they had been tested small male of different ages (five, nine or 13 days, whereas the age of the big males kept constant (five days. Experiments of copula success in the 1:1 ratio had been also carried through in field cage. It was prove that the big males had taken advantage in all the parameters analyzed in laboratory, emitting pheromone and having a bigger copula success, exactly when the age of the small males was varied. The size effect was so significant, that in the ratio of 1 big male for 10 small males, the females had still chosen the big males. In field cage, the results had been similar to the ones of laboratory.

  1. Improving the sterile sperm identification method for its implementation in the Area-wide Sterile Insect Technique Program against Ceratitis capitata (Diptera: Tephritidae) in Spain.

    Science.gov (United States)

    Juan-Blasco, M; Urbaneja, A; San Andrés, V; Castañera, P; Sabater-Muñoz, B

    2013-12-01

    The success of sterile males in area-wide sterile insect technique (aw-SIT) programs against Ceratitis capitata (Wiedemann) is currently measured by using indirect methods as the wild:sterile male ratio captured in monitoring traps. In the past decade, molecular techniques have been used to improve these methods. The development of a polymerase chain reaction-restriction fragment-length polymorphism-based method to identify the transfer of sterile sperm to wild females, the target of SIT, was considered a significant step in this direction. This method relies on identification of sperm by detecting the presence of Y chromosomes in spermathecae DNA extract complemented by the identification of the genetic origin of this sperm: Vienna-8 males or wild haplotype. However, the application of this protocol to aw-SIT programs is limited by handling time and personnel cost. The objective of this work was to obtain a high-throughput protocol to facilitate the routine measurement in a pest population of sterile sperm presence in wild females. The polymerase chain reaction-restriction fragment-length polymorphism markers previously developed were validated in Mediterranean fruit fly samples collected from various locations worldwide. A laboratory protocol previously published was modified to allow for the analysis of more samples at the same time. Preservation methods and preservation times commonly used for Mediterranean fruit fly female samples were assessed for their influence on the correct molecular detection of sterile sperm. This high-throughput methodology, as well as the results of sample management presented here, provide a robust, efficient, fast, and economical sterile sperm identification method ready to be used in all Mediterranean fruit fly SIT programs.

  2. Mitochondrial Single Nucleotide Polymorphisms in Ceratitis capitata (Diptera: Tephritidae) Can Distinguish Sterile, Released Flies from Wild Flies in Various Regions of the World.

    Science.gov (United States)

    Parubrub, Arlene; Reyes, Ruel; Smallridge, Catherine J; Woods, Bill; Haymer, David

    2015-02-01

    In areas infested with pest species such as the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), many programs rely heavily on the sterile insect technique (SIT) as a form of biological control. However, when SIT treatments are used both for control of established infestations and for occasional outbreaks, for several reasons, programs are often unable to adequately quantify the success of this approach. Chief among these are difficulties associated with reliably and rapidly determining the strain of origin of males recaptured during and after the SIT program. In this study, we describe the use of a DNA-based marker that can be used to rapidly and reliably distinguish males originating from the two sterile strains that are most widely used in SIT rearing facilities from males originating from wild strains of various regions of the world. This method uses polymerase chain reaction amplification of material from individual specimens to directly analyze DNA sequence variants found within a portion of the mitochondrial ND4 subunit 4 (ND4) gene to identify single nucleotide polymorphisms (SNPs) that are diagnostic of different strains. Specifically, the SNPs described here reliably distinguish individual flies originating from the Vienna 7 and Vienna 8 strains used for sterile release from wild flies infesting various areas including Western Australia, Guatemala, and Hawaii. The availability of such markers for determination of the strain of origin of specimens, either from whole specimens or body parts (including their sperm), has great potential to improve the ability to monitor and quantify the success of any sterile release program.

  3. Stage and cell-specific expression and intracellular localization of the small heat shock protein Hsp27 during oogenesis and spermatogenesis in the Mediterranean fruit fly, Ceratitis capitata.

    Science.gov (United States)

    Economou, Katerina; Kotsiliti, Elena; Mintzas, Anastassios C

    2017-01-01

    The cell-specific expression and intracellular distribution of the small heat protein Hsp27 was investigated in the ovaries and testes of the Mediterranean fruit fly, Ceratitis capitata (medfly), under both normal and heat shock conditions. For this study, a gfp-hsp27 strain was used to detect the chimeric protein by confocal microscopy. In unstressed ovaries, the protein was expressed throughout egg development in a stage and cell-specific pattern. In germarium, the protein was detected in the cytoplasm of the somatic cells in both unstressed and heat-shocked ovaries. In the early stages of oogenesis of unstressed ovaries, the protein was mainly located in the perinuclear region of the germ cells and in the cytoplasm of the follicle cells, while in later stages (9-10) it was distributed in the cytoplasm of the germ cells. In late stages (12-14), the protein changed localization pattern and was exclusively associated with the nuclei of the somatic cells. In heat shocked ovaries, the protein was mainly located in the nuclei of the somatic cells throughout egg chamber's development. In unstressed testes, the chimeric protein was detected in the nuclei of primary spermatocytes and in the filamentous structures of spermatid bundles, called actin cones. Interestingly, after a heat shock, the protein presented the same cell-specific localization pattern as in unstressed testes. Furthermore, the protein was also detected in the nuclei of the epithelial cells of the deferent duct, the accessory glands and the ejaculatory bulb. Our data suggest that medfly Hsp27 may have cell-specific functions, especially in the nucleus. Moreover, the association of this protein to actin cones during spermatid individualization, suggests a possible role of the protein in the formation and stabilization of actin cones.

  4. Diet-induced over-expression of flightless-I protein and its relation to flightlessness in Mediterranean fruit fly, Ceratitis capitata.

    Science.gov (United States)

    Cho, Il Kyu; Chang, Chiou Ling; Li, Qing X

    2013-01-01

    The Mediterranean fruit fly (medfly), Ceratitis capitata is among the most economically important pests worldwide. Understanding nutritional requirement helps rearing healthy medfly for biocontrol of its population in fields. Flight ability is a high priority criterion. Two groups of medfly larvae were reared with two identical component diets except one with fatty acids (diet A) and another without it (diet B). Adults from larvae reared on diet B demonstrated 20±8% of normal flight ability, whereas those from larvae reared on diet A displayed full flight ability of 97±1%. Proteomes were profiled to compare two groups of medfly pupae using shotgun proteomics to study dietary effects on flight ability. When proteins detected in pupae A were compared with those in pupae B, 233 and 239 proteins were, respectively, under- and over-expressed in pupae B, while 167 proteins were overlapped in both pupae A and B. Differential protein profiles indicate that nutritional deficiency induced over-expression of flightless-I protein (fli-I) in medfly. All proteins were subjected to Ingenuity Pathway Analysis (IPA) to create 13 biological networks and 17 pathways of interacting protein clusters in human ortholog. Fli-I, leucine-rich repeat (LRR)-containing G protein-coupled receptor 2, LRR protein soc-2 and protein wings apart-like were over-expressed in pupae B. Inositol-1,4,5-trisphosphate receptor, protocadherin-like wing polarity protein stan and several Wnt pathway proteins were under-expressed in pupae B. These results suggest down-regulation of the Wnt/wingless signaling pathway, which consequently may result in flightlessness in pupae B. The fli-I gene is known to be located within the Smith-Magenis syndrome (SMS) region on chromosome 17, and thus, we speculate that nutritional deficiency might induce over-expression of fli-I (or fli-I gene) and be associated with human SMS. However, more evidence would be needed to confirm our speculation.

  5. Analysis of Seasonal Risk for Importation of the Mediterranean Fruit Fly, Ceratitis capitata (Diptera: Tephritidae), via Air Passenger Traffic Arriving in Florida and California.

    Science.gov (United States)

    Szyniszewska, A M; Leppla, N C; Huang, Z; Tatem, A J

    2016-09-04

    The Mediterranean fruit fly, Ceratitis capitata (Wiedemann), is one of the most economically damaging pests in the world and has repeatedly invaded two major agricultural states in the United States, Florida and California, each time requiring costly eradication. The Mediterranean fruit fly gains entry primarily in infested fruit carried by airline passengers and, since Florida and California each receive about 13 million international passengers annually, the risk of Mediterranean fruit fly entering the United States is potentially very high. The risk of passengers bringing the pest into Florida or California from Mediterranean fruit fly-infested countries was determined with two novel models, one estimated seasonal variation in airline passenger number and the other defined the seasonal and spatial variability in Mediterranean fruit fly abundance. These models elucidated relationships among the risk factors for Mediterranean fruit fly introduction, such as amount of passenger traffic, routes traveled, season of travel, abundance of Mediterranean fruit fly in countries where flights departed, and risk of the pest arriving at destination airports. The risk of Mediterranean fruit fly being introduced into Florida was greatest from Colombia, Brazil, Panama, Venezuela, Argentina, and Ecuador during January-August, whereas primarily the risk to California was from Brazil, Panama, Colombia, and Italy in May-August. About three times more Mediterranean fruit flies were intercepted in passenger baggage at airports in Florida than California, although the data were compromised by a lack of systematic sampling and other limitations. Nevertheless, this study achieved the goal of analyzing available data on seasonal passenger flow and Mediterranean fruit fly population levels to determine when surveillance should be intensified at key airports in Florida and California.

  6. Machos Virgens e Acasalados de Ceratitis capitata Wiedemann (Diptera: Tephritidae Apresentam o mesmo Sucesso de Cópula e a mesma Capacidade de Inibição de Recópula das Fêmeas?

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    Luiza Santana

    2014-08-01

    Virgin and Mated Males of Ceratitis capitata Wiedemann (Diptera: Tephritidae Have the Same Mating Success and the Same Ability to Inhibit Female Remating? Abstract. Ceratitis capitata Wiedemann is a polyphagous species that damages fruits and affects their production and consumption. One of the techniques to manage this pest is the Sterile Insect Technique, which consists in releasing sterile males in nature to compete with wild males for mating. The success of this technique is associated with the ability of sterile male in being selected by the female and in preventing female remating with other males. This paper aims to evaluate the influence of male reproductive status in mating success and in female remating inhibition. Tests for evaluating the latency to mate and copula duration were performed to evaluate latency to mate and copula duration based on different male status. In remating inhibition tests, females mated with virgin and mated males, were exposed to other males one day after the first mating so the rate of remating could be evaluated. The results showed that males of different reproductive status had no differences in mating success and in female remating inhibition. The latency to mate and copula duration were similar for both male status as well. Our results suggest that, assuming that the sterile males have the same basic biology of no sterile males, in SIT, after released in nature, mated males can have the same success in mating and female remating inhibition as virgin males.

  7. In vivo bioinsecticidal activity toward Ceratitis capitata (fruit fly) and Callosobruchus maculatus (cowpea weevil) and in vitro bioinsecticidal activity toward different orders of insect pests of a trypsin inhibitor purified from tamarind tree (Tamarindus indica) seeds.

    Science.gov (United States)

    Araújo, Carina L; Bezerra, Ingrid W L; Oliveira, Adeliana S; Moura, Fabiano T; Macedo, Leonardo L P; Gomes, Carlos E M; Barbosa, Aulus E A D; Macedo, Francisco P; Souza, Tánia M S; Franco, Octavio L; Bloch-J, Carlos; Sales, Mauricio P

    2005-06-01

    A proteinaceous inhibitor with high activity against trypsin-like serine proteinases was purified from seeds of the tamarind tree (Tamarindus indica) by gel filtration on Shephacryl S-200 followed by a reverse-phase HPLC Vidac C18 TP. The inhibitor, called the tamarind trypsin inhibitor (TTI), showed a Mr of 21.42 kDa by mass spectrometry analysis. TTI was a noncompetitive inhibitor with a Ki value of 1.7 x 10(-9) M. In vitro bioinsecticidal activity against insect digestive enzymes from different orders showed that TTI had remarkable activity against enzymes from coleopteran, Anthonomus grandis (29.6%), Zabrotes subfasciatus (51.6%), Callosobruchus maculatus (86.7%), Rhyzopertha dominica(88.2%), and lepidopteron, Plodia interpuncptella (26.7%), Alabama argillacea (53.8%), and Spodoptera frugiperda (75.5%). Also, digestive enzymes from Diptera, Ceratitis capitata (fruit fly), were inhibited (52.9%). In vivo bioinsecticidal assays toward C. capitata and C. maculatus larvae were developed. The concentration of TTI (w/w) in the artificial seed necessary to cause 50% mortality (LD50) of larvae was 3.6%, and that to reduce mass larvae by 50.0% (ED50) was 3.2%. Furthermore, the mass C. capitata larvae were affected at 53.2% and produced approximately 34% mortality at a level of 4.0% (w/w) of TTI incorporated in artificial diets.

  8. Compatibility of entomopathogenic nematodes and aqueous plant extracts aiming at the control of fruit fly Ceratitis capitata (Wiedemann (Diptera: TephritidaeCompatibilidade de nematóides entomopatogênicos e extratos vegetais aquosos visando o controle da mosca-das-frutas Ceratitis capitata (Wiedemann (Diptera: Tephritidae

    Directory of Open Access Journals (Sweden)

    Cristhiane Rohde

    2013-06-01

    Full Text Available Currently, the fruit fly Ceratitis capitata (Diptera: Tephritidae has been controlled mainly by the chemical method, which is responsible for environmental and public health impacts. It has often been ineffective due to development of resistant insect populations. Thus, it has necessary to research new effective and less impacting control forms. In this sense, the use of entomopathogenic nematodes and plant extracts has been effective for controlling this pest. However, studies are needed to assess the compatibility between these methods, aiming at their use in integrated management programs for this pest. The aim of this study was to evaluate the compatibility of the nematodes Steinernema carpocapsae ALL and Heterorhabditis sp. JPM4 with aqueous extracts prepared from dried plant of cinnamon leaf, twig and fruit (Melia azedarach, rue leaf (Ruta graveolens, ginger (Zingiber officinale and garlic (Allium sativum for the control of C. capitata. The bioassay was carried out in completely randomized design with four replicates per treatment. Each replication consisted of a glass tube containing 1 mL of plant extract 40% w/v and 1 mL suspension of entomopathogenic nematodes with 1800 JI/mL for S. carpocapsae ALL and 600 JI/mL for Heterorhabditis sp. JPM4. The viability and infectivity of this nematode were evaluated on C. capitata larvae after 48 and 120 hours. It was found that all extracts reduced the viability and infectivity of both nematodes and they were incompatible after 120 hours of exposure. The nematode Heterorhabditis sp. JPM4 was more sensitive than the S. carpocapsae ALL as it showed, in the first 48 hours, a reduction in the viability and infectivity of more than 80 and 75%, respectively, when exposed to all the extracts except the ginger.A mosca-das-frutas Ceratitis capitata (Diptera: Tephritidae tem sido controlada, principalmente, pelo método químico, que é o responsável por impactos ambientais e na saúde pública e, muitas

  9. Diet-induced over-expression of flightless-I protein and its relation to flightlessness in Mediterranean fruit fly, Ceratitis capitata.

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    Il Kyu Cho

    Full Text Available The Mediterranean fruit fly (medfly, Ceratitis capitata is among the most economically important pests worldwide. Understanding nutritional requirement helps rearing healthy medfly for biocontrol of its population in fields. Flight ability is a high priority criterion. Two groups of medfly larvae were reared with two identical component diets except one with fatty acids (diet A and another without it (diet B. Adults from larvae reared on diet B demonstrated 20±8% of normal flight ability, whereas those from larvae reared on diet A displayed full flight ability of 97±1%. Proteomes were profiled to compare two groups of medfly pupae using shotgun proteomics to study dietary effects on flight ability. When proteins detected in pupae A were compared with those in pupae B, 233 and 239 proteins were, respectively, under- and over-expressed in pupae B, while 167 proteins were overlapped in both pupae A and B. Differential protein profiles indicate that nutritional deficiency induced over-expression of flightless-I protein (fli-I in medfly. All proteins were subjected to Ingenuity Pathway Analysis (IPA to create 13 biological networks and 17 pathways of interacting protein clusters in human ortholog. Fli-I, leucine-rich repeat (LRR-containing G protein-coupled receptor 2, LRR protein soc-2 and protein wings apart-like were over-expressed in pupae B. Inositol-1,4,5-trisphosphate receptor, protocadherin-like wing polarity protein stan and several Wnt pathway proteins were under-expressed in pupae B. These results suggest down-regulation of the Wnt/wingless signaling pathway, which consequently may result in flightlessness in pupae B. The fli-I gene is known to be located within the Smith-Magenis syndrome (SMS region on chromosome 17, and thus, we speculate that nutritional deficiency might induce over-expression of fli-I (or fli-I gene and be associated with human SMS. However, more evidence would be needed to confirm our speculation.

  10. Evaluation of Quality Production Parameters and Mating Behavior of Novel Genetic Sexing Strains of the Mediterranean Fruit Fly Ceratitis capitata (Wiedemann (Diptera: Tephritidae.

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    Polychronis Rempoulakis

    Full Text Available The Mediterranean fruit fly Ceratitis capitata (Wiedemann (Diptera: Tephritidae is one of the most important pest of fruits and vegetables in tropical and subtropical countries. The sterile insect technique (SIT as a component of area-wide integrated pest management (AW-IPM approaches is being used for the successful management of this pest. VIENNA 8 is a genetic sexing strain (GSS that has a white pupae (wp and temperature sensitive lethal (tsl mutation, the latter killing all female embryos when eggs are exposed to high temperatures (34°C. The use of this GSS permits production and the release of only males which has increased the cost effectiveness of the SIT several fold for this pest. An efficient method of identification of recaptured sterile males can further increase the cost effectiveness of the SIT for this pest. Therefore, VIENNA 8-Sergeant2 (Sr2 strain and the transgenic strain VIENNA 8-1260 having visible markers were constructed. All three strains were evaluated for egg production, egg hatch, and egg sterility parameters under semi mass-rearing conditions and mating competitiveness in field cages. VIENNA 8-1260 females produced significantly fewer eggs as compared with the two other strains, which produced similar numbers of eggs. However, egg hatch of all strains was similar. Egg hatch of eggs produced by untreated females that had mated with adult males that had been irradiated with 100 Gy as pupae 2 days before emergence, was different for the three strains, i.e., egg hatch of 0.63%, 0.77%, 0.89% for VIENNA 8, VIENNA 8-1260, and VIENNA 8-Sr2, respectively. Differences in male mating competitiveness of the three strains against wild-type males were gradually reduced with successive generations under semi mass-rearing conditions. However, VIENNA 8 males adapted faster to laboratory conditions as compared with VIENNA 8-Sr2 and VIENNA 8-1260 males with respect to mating competitiveness. VIENNA 8 males of the F10 generation were

  11. Evaluation of Quality Production Parameters and Mating Behavior of Novel Genetic Sexing Strains of the Mediterranean Fruit Fly Ceratitis capitata (Wiedemann) (Diptera: Tephritidae).

    Science.gov (United States)

    Rempoulakis, Polychronis; Taret, Gustavo; Haq, Ihsan Ul; Wornayporn, Viwat; Ahmad, Sohel; Sto Tomas, Ulysses; Dammalage, Thilakasiri; Gembinsky, Keke; Franz, Gerald; Cáceres, Carlos; Vreysen, Marc J B

    2016-01-01

    The Mediterranean fruit fly Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) is one of the most important pest of fruits and vegetables in tropical and subtropical countries. The sterile insect technique (SIT) as a component of area-wide integrated pest management (AW-IPM) approaches is being used for the successful management of this pest. VIENNA 8 is a genetic sexing strain (GSS) that has a white pupae (wp) and temperature sensitive lethal (tsl) mutation, the latter killing all female embryos when eggs are exposed to high temperatures (34°C). The use of this GSS permits production and the release of only males which has increased the cost effectiveness of the SIT several fold for this pest. An efficient method of identification of recaptured sterile males can further increase the cost effectiveness of the SIT for this pest. Therefore, VIENNA 8-Sergeant2 (Sr2) strain and the transgenic strain VIENNA 8-1260 having visible markers were constructed. All three strains were evaluated for egg production, egg hatch, and egg sterility parameters under semi mass-rearing conditions and mating competitiveness in field cages. VIENNA 8-1260 females produced significantly fewer eggs as compared with the two other strains, which produced similar numbers of eggs. However, egg hatch of all strains was similar. Egg hatch of eggs produced by untreated females that had mated with adult males that had been irradiated with 100 Gy as pupae 2 days before emergence, was different for the three strains, i.e., egg hatch of 0.63%, 0.77%, 0.89% for VIENNA 8, VIENNA 8-1260, and VIENNA 8-Sr2, respectively. Differences in male mating competitiveness of the three strains against wild-type males were gradually reduced with successive generations under semi mass-rearing conditions. However, VIENNA 8 males adapted faster to laboratory conditions as compared with VIENNA 8-Sr2 and VIENNA 8-1260 males with respect to mating competitiveness. VIENNA 8 males of the F10 generation were equally

  12. Biological and morphological aspects of Diachasmimorpha longicaudata (Ashmead, 1905) (Hymenoptera: Braconidae) on irradiated larva of Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae);Aspectos biologicos e morfologicos de Diachasmimorpha longicaudata (Ashmead, 1905) (Hymenoptera: Braconidae) criado em larvas irradiadas de Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae)

    Energy Technology Data Exchange (ETDEWEB)

    Valle, Giuliana Etore do

    2006-07-01

    The objective of this work was evaluate some biological and morphological aspects of Diachasmimorpha longicaudata on irradiated and not irradiated larva of bisexual and tsl strains of Ceratitis capitata. The experiments were developed at the Laboratorio de Irradiacao de Alimentos e Radioentomologia (LIARE) of CENA/USP. For gamma radiation treatment it was used a Cobalt-60 source, model Gamma beam-650. Larva of bisexual strain were irradiated with 65 Gy and the tsl strain with 45 Gy. Experiments were carried out at room temperature of 25 +- 2 deg C, 75 +- 5 % RH and 14 hours of photo phase. After irradiation, the bisexual strain larva were exposed to adults of D. longicaudata for parasitization and parameters of percentage adult emergency and rate of male and female of D. longicaudata were observed. For tsl strain, were used larva from the first and the fourth collection only, and the following parameters were observed: percentage of adult emergency, rate of male and female, and the average unitary volume and weight of pupae at the 8{sup th} and 16 days after the parasitism date. Other parameters evaluated for tsl strain related to the parasitoid morphology such as, the length of the body, antenna, tibia and ovipositor. As result, both of the first and second experiments, the rate of parasitism was satisfactory, as well as showed larger incidence of females than males in parasitized larva, discarding the possibility that gamma radiation interfere in the sexual rate. No significant differences were observed on pupae volume. Some differences on pupae weight were observed at 8th and 16th day after the parasitism date, which should be associated to pre-emerging flies (8th day) and empty pupa (16th day). None significant difference was observed on morphological parameters, so one can conclude that gamma radiation treatment did not interfere in these quality parameters of the parasitoid. (author)

  13. Field trials of solid triple lure (trimedlure, methyl eugenol, raspberry ketone, and DDVP) dispensers for detection and male annihilation of Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae (Diptera: Tephritidae) in Hawaii.

    Science.gov (United States)

    Vargas, Roger I; Souder, Steven K; Mackey, Bruce; Cook, Peter; Morse, Joseph G; Stark, John D

    2012-10-01

    Solid Mallet TMR (trimedlure [TML], methyl eugenol [ME], raspberry ketone [RK]) wafers and Mallet CMR (ceralure, ME, RK, benzyl acetate) wafers impregnated with DDVP (2,2-dichlorovinyl dimethyl phosphate) insecticide were measured in traps as potential detection and male annihilation technique (MAT) devices. Comparisons were made with 1) liquid lure and insecticide formulations, 2) solid cones and plugs with an insecticidal strip, and 3) solid single and double lure wafers with DDVP for captures of Mediterranean fruit fly, Ceratitis capitata (Wiedemann); oriental fruit fly, Bactrocera dorsalis Hendel; and melon fly, B. cucurbitae Coquillett. Bucket and Jackson traps were tested in a coffee plantation near Eleele, Kauai Island, HI (trials at high populations) and avocado orchards near Kona, HI Island, HI (trials at low populations). Captures of all three species with Mallet TMR were not different from Mallet CMR; therefore, subsequent experiments did not include Mallet CMR because of higher production costs. In MAT trials near Eleele, HI captures in AWPM traps with Mallet TMR wafers were equal to any other solid lure (single or double) except the Mallet ME wafer. In survey trials near Kona, captures of C. capitata, B. cucurbitae, and B. dorsalis with Mallet TMR wafers were equal to those for the standard TML, ME, and C-L traps used in FL and CA. A solid Mallet TMR wafer is safer, more convenient to handle, and may be used in place of several individual lure and trap systems, potentially reducing costs of large survey and detection programs in Florida and California, and MAT programs in Hawaii.

  14. Etude de l’activité antiparasitaire et antimicrobienne des extraits de zests d’oranges Citrus sinensis var.Washington navel sur le ravageur Ceratitis capitata et la mycoflore qui lui est associée.

    OpenAIRE

    Benazzouz, Fatima zohra

    2015-01-01

    Ceratitis capitata سمحت لنا الدراسة البیوایكولوجیة لذبالة الفواكھ Penicillium sp. Aspergillus sp et Monilia أربع أنواع (Alternaria sp.,. الفطریات الضارة المتعلقة بھا، سمحت بتعریف ثمانیة أنواع فطریة sp). ثم تلیھا مكونات فینولیة أخرى (Tanins ) بالمقابل دراسة قدرة البرتقال على مقاومة ھذه القطریات بینت أن المكافحة تبدأ بإفراز مركبات فینولیة تركیبیة Ceratitis ) دراسة خلاصة قشرة البرتقال و الزیوت الطیارة الخاصة بھا على العزلات الفطریة و یرقات الذبابة و الذبابة نفسھا .(Flavonoides) حاثة ...

  15. Determination of the dose of gamma radiation sterilization for assessment of biological parameters of male Ceratitis capitada (Diptera: Tephritidae), tsl - Vienna 8 strain; Determinacao da dose de radiacao gama esterilizante pela avaliacao dos parametros biologicos de machos de Ceratitis capitata (Diptera: Tephritidae), linhagem tsl - Vienna 8

    Energy Technology Data Exchange (ETDEWEB)

    Rocha, Aline Cristina Pereira da

    2011-07-01

    The Vienna-8, tsl (temperature sensitive lethal) strain of Ceratitis capitata, by presenting mutations that facilitate the mass rearing and release only of sterile males in the field, has been used in (Sterile Insect Technique) programmes. The objective of this study was to determine the radiation dose that provides the highest level of sterility for Vienna-8, tsl males assessing their biological parameters that indicate the quality of sterile males to be released. Brown pupae (males) of the tsl strain were obtained from the mass rearing of the Food Irradiation and Radio entomology laboratory of CENA/USP, and they were irradiated (with gamma radiation - {sup 60}Co) 24 hours before the emergence at rates of 0, 30, 60, 90 and 120 Gy. The determination of the sterilizing dose was based on fertility of sexually mature females of the bisexual strain and not irradiated, mated with males of different treatments. Eggs were collected daily during 6 days, were counted and it was possible to estimate fecundity, and assess the hatching rate. The emergence and flight ability were determined by following the protocol of quality control manual for FAO/IAEA/USDA (2003). To assess the longevity under nutritional stress, the insects were kept a period of 48 h after emergence in the absence of water and food, and after this period, mortality was recorded. The size of the testes (left and right) was obtained by dissecting irradiated and non-irradiated males at the eighth day of life, and measure the testes in an ocular micrometer, considering the maximum length and width of each sample. To determine the sperm number was necessary to dissect the males and break their testicles. No difference was observed in emergence rate, flight ability and longevity of irradiated and non-irradiated males, nor in the fecundity of females mated with males of different treatments. The sterilizing dose that resulted in lower fertility of females was 120 Gy, with 1.5% hatching. Considering the parameters

  16. Genetics differentiation in the Mediterranean fruit fly, Ceratitis capitata (Wiedemann)%地中海实蝇地理种群遗传分化研究

    Institute of Scientific and Technical Information of China (English)

    林丽莉; 曾玲; 梁广文; 吴佳教; 顾渝娟; 刘海军; 胡学难

    2010-01-01

    以mtDNA的CO Ⅰ基因的部分序列作为分子标记,研究了来自安哥拉、黎巴嫩、刚果共和国、尼日利亚、贝宁、多哥、加纳、科特迪瓦、南非、埃及、法国、约旦、秘鲁、智利、美国佛罗里达和美国夏威夷等16个国家和地区共75个个体的地中海实蝇Ceratitis capitaza地理种群序列特征和单倍型特征,并分析了各种群间的遗传分化水平和进化关系,结果表明,在供试的样品中发现单倍型的数目是29,其中有5种为共享倍型.地中海实蝇各地理种群的遗传多态性水平差异较大,总体遗传距离D等于0.007.非洲南撒哈拉区(安哥拉、刚果、尼日利亚、贝宁、多哥、加纳、科特迪瓦)的种群的单倍型数、单倍型百分比和群内遗传距离等遗传多态性参数值都高于其它供试地区,多样性明显较其他地区丰富.

  17. The gene chip detection technique for the Mediterranean fly, Ceratitis capitata, and its related species (Diptera: Tephritidae)%地中海实蝇及其近缘种基因芯片检测研究

    Institute of Scientific and Technical Information of China (English)

    李文芬; 余道坚; 颜亨梅; 李建光; 徐浪; 任鲁风

    2008-01-01

    本研究选择线粒体DNA(mtDNA)细胞色素氧化酶Ⅰ基因(COⅠ)为分子标记基因,以双翅目实蝇科昆虫DNA序列为目标,建立了我国进境植物检疫害虫地中海实蝇Ceratitis capitata、芒果小条实蝇C.cosyra和纳塔尔小条实蝇C.rosa等生物芯片检测方法.地中海实蝇及其近缘种检测芯片由检测探针(实蝇科通用探针1条.小条实蝇属通用探针1条,地中海实蝇、芒果小条实蝇和纳塔尔小条实蝇近缘种探针2条和种特异探针4条)、质控探针(定位点探针、阳性质控、阴性质控和空白对照探针各1条)组成.芯片检测结果表明,检测探针特异性强,能实现上述3种实蝇的种类快速区分和准确鉴定;检测方法稳定性好,地中海实蝇不同虫态(卵、幼虫、蛹和成虫)和不同地理种群检测结果完全一致.地中海实蝇生物芯片检测技术将为我国进口果蔬中检疫性实蝇快速筛查和种类鉴定提供检测方法,同时,还可应用到其他属的实蝇以及相关害虫的检疫中,为有害生物的快速鉴定提供了新方法.

  18. Internetmarkten: wie profiteert?

    NARCIS (Netherlands)

    M.C.W. Janssen (Maarten); J.L. Moraga-Gonzalez (José Luis)

    2000-01-01

    textabstractEconomen lijken het er over eens dat het internet positieve welvaartseffecten kan hebben. Maar of het werkelijk de mogelijkheden biedt om tot een perfecte markt te komen, blijft de vraag. Werpt het internet geen nieuwe barrières op? En aan wie valt de extra welvaart toe? Over visionairs

  19. Adaptation to divergent larval diets in the medfly, Ceratitis capitata.

    Science.gov (United States)

    Leftwich, Philip T; Nash, William J; Friend, Lucy A; Chapman, Tracey

    2017-02-01

    Variation in diet can influence the timing of major life-history events and can drive population diversification and ultimately speciation. Proximate responses of life histories to diet have been well studied. However, there are scant experimental data on how organisms adapt to divergent diets over the longer term. We focused on this omission by testing the responses of a global pest, the Mediterranean fruitfly, to divergent selection on larval diets of different nutritional profiles. Tests conducted before and after 30 generations of nutritional selection revealed a complex interplay between the effects of novel larval dietary conditions on both plastic and evolved responses. There were proximate-only responses to the larval diet in adult male courtship and the frequency of copulation. Males on higher calorie larval diets consistently engaged in more bouts of energetic courtship. In contrast, following selection, larval development time, and egg to adult survival showed evidence of evolved divergence between diet regimes. Adult body size showed evidence for adaptation, with flies being significantly heavier when reared on their "own" diet. The results show the multifaceted responses of individuals to dietary selection and are important in understanding the extreme generalism exhibited by the medfly.

  20. Proton conducting cerate ceramics

    Energy Technology Data Exchange (ETDEWEB)

    Coffey, G.W.; Pederson, L.R.; Armstrong, T.R.; Bates, J.L.; Weber, W.J. [Pacific Northwest Lab., Richland, WA (United States)

    1995-08-01

    Cerate perovskites of the general formula AM{sub x}Ce{sub 1-x}O{sub 3-{delta}}, where A = Sr or Ba and where M = Gd, Nd, Y, Yb or other rare earth dopant, are known to conduct a protonic current. Such materials may be useful as the electrolyte in a solid oxide fuel cell operating at intermediate temperatures, as an electrochemical hydrogen separation membrane, or as a hydrogen sensor. Conduction mechanisms in these materials were evaluated using dc cyclic voltammetry and mass spectrometry, allowing currents and activation energies for proton, electron, and oxygen ion contributions to the total current to be determined. For SrYb{sub 0.05}Ce{sub 0.95}O{sub 3-{delta}}, one of the best and most environmentally stable compositions, proton conduction followed two different mechanisms: a low temperature process, characterized by an activation energy of 0.42{+-}0.04 eV, and a high temperature process, characterized by an activation energy of 1.38{+-}0.13 eV. It is believed that the low temperature process is dominated by grain boundary conduction while bulk conduction is responsible for the high temperature process. The activation energy for oxygen ion conduction (0.97{+-}0.10 eV) agrees well with other oxygen conductors, while that for electronic conduction, 0.90{+-}0.09 eV, is affected by a temperature-dependent electron carrier concentration. Evaluated by direct measurement of mass flux through a dense ceramic with an applied dc field, oxygen ions were determined to be the majority charge carrier except at the lowest temperatures, followed by electrons and then protons.

  1. Wie Nationalismus das Geschlecht macht

    Directory of Open Access Journals (Sweden)

    Ruben Marc Hackler

    2006-07-01

    Full Text Available Heidrun Zettelbauer untersucht in ihrer Dissertation den Zusammenhang von Geschlecht und Nation in den völkischen Diskursen der Habsburgermonarchie zwischen 1880 und 1918. Anhand von Vereinspublikationen weist sie überzeugend nach, wie geschlechtsspezifische Zuschreibungen durch die nationalistische Bewegung befestigt und vorangetrieben worden sind. Gleichzeitig zeigt sie, dass die Bewegung auf die Integration von Frauen angewiesen war, was die Möglichkeit für Frauen bot, sich im Verein politisch zu betätigen und so die bürgerliche Auffassung weiblicher Subjektivität zu unterlaufen.

  2. Proton-conducting cerate ceramics

    Energy Technology Data Exchange (ETDEWEB)

    Pederson, L.R.; Coffey, G.W.; Bates, J.L.; Weber, W.J. [Pacific Northwest National Lab., Richland, WA (United States)

    1996-08-01

    Single-cell solid oxide fuel cells were constructed using strontium cerate as the electrolyte and their performance tested. Like certain zirconates, hafnates, and tantalates, the cerate perovskites are among a class of solid electrolytes that conduct protons at elevated temperatures. Depending on the temperature and chemical environment, these ceramics also support electronic and oxygen ion currents. A maximum power output of {approx}100 mW per cm{sup 2} electrolyte surface area was obtained at 900{degrees}C using 4% hydrogen as the fuel and air as the oxidant. A series of rare earth/ceria/zirconia were prepared and their electrical properties characterized. Rare earth dopants included ytterbia, yttria, terbia, and europia. Ionic conductivities were highest for rare earth/ceria and rare earth zirconia compositions; a minimum in ionic conductivity for all series were found for equimolar mixtures of ceria and zirconia. Cerium oxysulfide is of interest in fossil energy applications because of its high chemical stability and refractory nature. An alternative synthesis route to preparing cerium oxysulfide powders has been developed using combustion techniques.

  3. Global assessment of seasonal potential distribution of Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae.

    Directory of Open Access Journals (Sweden)

    Anna M Szyniszewska

    Full Text Available The Mediterranean fruit fly (Medfly is one of the world's most economically damaging pests. It displays highly seasonal population dynamics, and the environmental conditions suitable for its abundance are not constant throughout the year in most places. An extensive literature search was performed to obtain the most comprehensive data on the historical and contemporary spatio-temporal occurrence of the pest globally. The database constructed contained 2328 unique geo-located entries on Medfly detection sites from 43 countries and nearly 500 unique localities, as well as information on hosts, life stages and capture method. Of these, 125 localities had information on the month when Medfly was recorded and these data were complemented by additional material found in comprehensive databases available online. Records from 1980 until present were used for medfly environmental niche modeling. Maximum Entropy Algorithm (MaxEnt and a set of seasonally varying environmental covariates were used to predict the fundamental niche of the Medfly on a global scale. Three seasonal maps were also produced: January-April, May-August and September-December. Models performed significantly better than random achieving high accuracy scores, indicating a good discrimination of suitable versus unsuitable areas for the presence of the species.

  4. Leg impairement magnifies reproductive costs in male Mediterranean fruit flies, Ceratitis capitata

    Science.gov (United States)

    Injuries frequently accumulate with age in nature. Despite the commonality of injury and the resulting impairment, there are limited experimental data for the effects of impairment on life history trade-offs between reproduction and survival in insects. We tested the effects of artificial injury and...

  5. Global assessment of seasonal potential distribution of Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae).

    Science.gov (United States)

    Szyniszewska, Anna M; Tatem, Andrew J

    2014-01-01

    The Mediterranean fruit fly (Medfly) is one of the world's most economically damaging pests. It displays highly seasonal population dynamics, and the environmental conditions suitable for its abundance are not constant throughout the year in most places. An extensive literature search was performed to obtain the most comprehensive data on the historical and contemporary spatio-temporal occurrence of the pest globally. The database constructed contained 2328 unique geo-located entries on Medfly detection sites from 43 countries and nearly 500 unique localities, as well as information on hosts, life stages and capture method. Of these, 125 localities had information on the month when Medfly was recorded and these data were complemented by additional material found in comprehensive databases available online. Records from 1980 until present were used for medfly environmental niche modeling. Maximum Entropy Algorithm (MaxEnt) and a set of seasonally varying environmental covariates were used to predict the fundamental niche of the Medfly on a global scale. Three seasonal maps were also produced: January-April, May-August and September-December. Models performed significantly better than random achieving high accuracy scores, indicating a good discrimination of suitable versus unsuitable areas for the presence of the species.

  6. Effect of dietary components on larval life history characteristics in the medfly (Ceratitis capitata: Diptera, Tephritidae.

    Directory of Open Access Journals (Sweden)

    William J Nash

    Full Text Available BACKGROUND: The ability to respond to heterogenous nutritional resources is an important factor in the adaptive radiation of insects such as the highly polyphagous Medfly. Here we examined the breadth of the Medfly's capacity to respond to different developmental conditions, by experimentally altering diet components as a proxy for host quality and novelty. METHODOLOGY/PRINCIPAL FINDINGS: We tested responses of larval life history to diets containing protein and carbohydrate components found in and outside the natural host range of this species. A 40% reduction in the quantity of protein caused a significant increase in egg to adult mortality by 26.5%±6% in comparison to the standard baseline diet. Proteins and carbohydrates had differential effects on larval versus pupal development and survival. Addition of a novel protein source, casein (i.e. milk protein, to the diet increased larval mortality by 19.4%±3% and also lengthened the duration of larval development by 1.93±0.5 days in comparison to the standard diet. Alteration of dietary carbohydrate, by replacing the baseline starch with simple sugars, increased mortality specifically within the pupal stage (by 28.2%±8% and 26.2%±9% for glucose and maltose diets, respectively. Development in the presence of the novel carbohydrate lactose (milk sugar was successful, though on this diet there was a decrease of 29.8±1.6 µg in mean pupal weight in comparison to pupae reared on the baseline diet. CONCLUSIONS: The results confirm that laboratory reared Medfly retain the ability to survive development through a wide range of fluctuations in the nutritional environment. We highlight new facets of the responses of different stages of holometabolous life histories to key dietary components. The results are relevant to colonisation scenarios and key to the biology of this highly invasive species.

  7. Mass Rearing of Temperature Sensitive Genetic Sexing Strains in the Mediterranean Fruit Fly (Ceratitis Capitata)

    Energy Technology Data Exchange (ETDEWEB)

    Caceres, C. [FAO/IAEA Agriculture and Biotechnology Laboratory, Entomology Unit, A-2444 Seibersdorf (Austria)]. E-mail: c.caceres@iaea.org

    2002-09-15

    Genetic sexing strains (GSS) based on the temperature sensitive lethal(tsl) mutation are being used to produce sterile male medflies for large scale sterile insect technique (SIT) programmes for this pest. The use of male-only strains increases the overall efficiency of the technique. Currently more than 1.4 billion sterile male-only pupae are produced per week in different facilities around the world. Due to the mutations used to construct these strains, that is, translocations and selectable markers, they require different and more careful mass rearing procedures than do bisexual strains (BSS). The basic rearing technology has been developed and can be used to produce only males on a predictable basis to a level of 99.9% accuracy. If specific rearing procedures are followed, then tsl-based GSS has a rearing efficiency that is equal to that of a BSS and it is already know that males produced by the tsl-based GSS are of equal quality to males produced by BSS. Based on current rearing technology the cost of production of male pupae is about the same for both types of strain. This is due to the large colony that is required for the tsl-based GSS. This paper discusses the considerations that need to be taken into account during mass rearing of GSS and identifies the most efficient production processes that are currently available. (author)

  8. Voeding voor wie lang fit wil blijven

    NARCIS (Netherlands)

    Groot, de C.P.G.M.

    2012-01-01

    Goede voeding kan helpen ouderdomskwalen te voorkomen, ook voor wie al 50-plus is. En tot die categorie behoren steeds meer mensen, in Nederland en daarbuiten. Ook gaat met de jaren de behoefte aan sommige voedingsstoffen omhoog. Volop kansen voor productontwikkeling dus.

  9. Wie Let Er Op De Kleintjes?

    NARCIS (Netherlands)

    Menting, Ankie; Orobio De Castro, Bram

    2014-01-01

    Het onderliggende rapport betreft een verslag van het onderzoek dat door Dr. Ankie Menting en Prof. dr. Bram Orobio de Castro (Universiteit Utrecht), in opdracht van Humanitas, is uitgevoerd naar het project Wie Let Er Op De Kleintjes (WLEODK) van Gezin in Balans (Humanitas). In dit onderzoek, dat u

  10. Ceratitis cosyra, een Afrikaanse boorvlieg gevonden in Drenthe (Diptera: Tephritidae)

    NARCIS (Netherlands)

    Smit, J.T.; Aartsen, van B.

    2002-01-01

    Ceratitis cosyra, an African fruitfly found in the Dutch province of Drenthe (Diptera: Tephritidae) A single specimen of Ceratitis (Ceratalaspis) cosyra (Walker, 1849) was collected near Papenvoort (utm ld4768) with a malaisetrap in the period 4-6 september 1993 (leg. L. Witmond). Up till now it was

  11. 7 CFR 319.56-49 - Eggplant from Israel.

    Science.gov (United States)

    2010-01-01

    ... introduction of the following quarantine pests: Ceratitis capitata, Eutetranychus orientalis, Helicoverpa..., Ceratitis capitata) is required both inside and outside the pest-exclusionary structures. Trapping...

  12. 77 FR 34781 - Importation of Tomatoes From the Economic Community of West African States Into the Continental...

    Science.gov (United States)

    2012-06-12

    ... importation of tomatoes from various countries where the Mediterranean fruit fly (Medfly, Ceratitis capitata... quarantine pests: Bactrocera cucurbitae, B. invadens, Ceratitis capitata, C. rosa, Chrysodeixis...

  13. 75 FR 36347 - Determination of Pest-Free Areas in Mendoza Province, Argentina; Request for Comments

    Science.gov (United States)

    2010-06-25

    ... recognize additional areas as pest- free areas for Mediterranean fruit fly (Ceratitis capitata) in Argentina... being free of Ceratitis capitata, Mediterranean fruit fly (Medfly).\\1\\ Specifically, the Government...

  14. Wie kann man komplexe Themen wie Globalisierung oder europäische Integration unterrichten?

    OpenAIRE

    Müller, Ragnar

    2006-01-01

    Die Dissertation zeigt die Vermittlungsprobleme auf, die sich in der politischen Bildung bei komplexen Themen wie Globalisierung oder europäischer Integration stellen, und stellt einen neuen politikdidaktischen Ansatz zur Diskussion, der Politikverständnis in einer globalisierten Welt erreichen will. The Thesis analyses the problems of teaching complex political topics such as globalisation and European integration and presents a new approach to teaching politics in a globalized world.

  15. Particularities of Radiation Defect Formation in Ceramic Barium Cerate

    Science.gov (United States)

    Khromushin, I. V.; Aksenova, T. I.; Tuseev, T.; Munasbaeva, K. K.; Ermolaev, Yu V.; Ermolaev, V. N.; Seitov, A. S.

    2015-04-01

    The effects of irradiation with electrons, ions of noble gases (Ne, Ar, Kr) and oxygen on the structure and properties of neodymium-doped barium cerate have been studied using the methods of X-ray diffraction analysis, scanning electron and atomic force microscopy, thermal desorption spectroscopy. It was shown that irradiation by low-energy ions of noble gases stimulates the blistering processes on the sample surface, while the high-energy ions contribute to formation of the structures on the irradiated surface that resemble the various stages of spherulitegrowth. The similar structures were not observed in the case of irradiation with high-energy oxygen ions. According to the data on thermal desorption of water and oxygen molecules from the irradiated barium cerate it was supposed that irradiation by the noble gas ions promotes neodymium oxidation state change. It was noticed that the electron irradiation leads to the formation of the nano-sized acicular structures on the cerate surface.

  16. Response of Ceratitis capitata, Bactrocera dorsalis, and Bactrocera cucurbitae (Diptera:Tephritidae) to metabolic stress disinfection and disinfestation treatment

    Science.gov (United States)

    Metabolic stress disinfection and disinfestation (MSDD) is a postharvest treatment designed to control pathogens and arthropod pests on commodities that combines short cycles of low pressure/vacuum and high CO2 with ethanol vapor. Experiments were conducted to evaluate the effect of MSDD treatment o...

  17. THE BRINE SHRIMP (ARTEMIA SALINA) LETHALITY OF Brassica oleracea var. capitata

    OpenAIRE

    O.T. TÜZÜN, E. GÜRKAN, F. HIRLAK,

    2015-01-01

    This work covers up the bio-activities of the five fractions obtained from the ethanolic extract of Brassica oleracea var. capitata (Cruciferae).Key Words: Brassica oleracea var. capitata, Brine shrimp (Artemia salina)

  18. 78 FR 8435 - Importation of Fresh Citrus Fruit From Uruguay, Including Citrus Hybrids and Fortunella

    Science.gov (United States)

    2013-02-06

    ... (South American fruit fly) and Ceratitis capitata (Mediterranean fruit fly); two moths, Cryptoblabes..., Ceratitis capitata, Cryptoblabes gnidiella, Elsino australis, Gymnandrosoma aurantianum, and Xanthomonas.... capitata. If the prevalence rises above levels specified in the bilateral workplan, remedial measures...

  19. Als og wie - en udfordring for den syntaktiske analyse

    DEFF Research Database (Denmark)

    Holsting, Alexandra

    2011-01-01

    systematically govern one of the cases - accusative, dative or genitive - makes them hard to categorize. I shall present and discuss the different solutions suggested by eleven theoretical grammars and afterwards present an alternative solution based on a differentiation of als and wie for comparing on the one...

  20. Wat iedere journalist moet weten van marktonderzoek : Doorvragen bij wie

    NARCIS (Netherlands)

    de Leeuw, Edith

    2014-01-01

    Een gouden regel in de journalis-tiek vormen de vijf W’s (en een H). Een goede onderzoeksjournalist zal altijd vragen: wie, waarom, wat, waar, wanneer en hoe. Het zijn prima basisvragen, maar wanneer een journalist een item voorbereidt en daarbij gebruik maakt van marktonderzoek, moet er nog even do

  1. Lifespan of a Ceratitis fruit fly increases with higher altitude

    OpenAIRE

    2010-01-01

    Variation in lifespan may be linked to geographic factors. While latitudinal variation in lifespan has been studied for a number of species, altitude variation has received much less attention, particularly in insects. We measured the lifespan of different populations of the Natal fruit fly Ceratitis rosa along an altitudinal cline. For the different populations we first measured the residual longevity of wild flies by captive cohort approach and compared F1 generation from the same populatio...

  2. Antikoagulation bei Vorhofflimmern: Wie und mit welchem Medikament?

    Directory of Open Access Journals (Sweden)

    Gary T

    2015-01-01

    Full Text Available Direkte orale Antikoagulantien (DOAKs sind Alternativen zu Vitamin-K-Antagonisten (VKA bei Vorhofflimmerarrhythmie- (VHFA- Patienten und werden diese in dieser Indikation zu weiten Teilen ersetzen. Bei VHFA-Patienten mit Dialyse oder mechanischen Herzklappen sind VKA nach wie vor die einzige Option. Bei der Gabe von DOAKs sollte vor Therapieeinleitung auf das Vorhandensein von Begleiterkrankungen (z. B. Niereninsuffizienz, stattgehabte gastrointestinale Blutungen etc. sowie auf die Begleittherapie der Patienten (z. B. Amiodaron etc. geachtet werden, um die passende Substanz in der richtigen Dosierung zu wählen.

  3. Anticancer properties of Thymbra capitata and Helichrysum italicum essential oils

    Directory of Open Access Journals (Sweden)

    Juliana Poças

    2014-06-01

    It has long been recognised that some EOs have antimicrobial, antibacterial, antioxidant, antiviral, antimycotic, antiparasitic, insecticidal activities. However, studies into their anti-tumoral activities are scarce. This study, indicates that Thymbra capitata and Helichrysum italicum EOs exhibited antitumor activity against RKO and MCF7 cells, deserving further research into both the mechanism of the observed anti-tumour activity and potential cancer therapeutic applications.

  4. Hypolipidemic effects of aqueous extract of Acalypha capitata leaves in rats fed on high cholesterol diet

    Institute of Scientific and Technical Information of China (English)

    Nnodim Johnkennedy; Emejulu Adamma; Nwadike Constance Nnedimma

    2011-01-01

    Objective:To evaluate the hypolipidemic effects of aqueous extract of Acalypha capitata (A. capitata) leaves in rats fed on high cholesterol diet. Methods:Cholesterol diet was administered to Wistar rats at a dose of 40 mg per 0.2 mL 3 times daily for 14 days while the control received distilled water. These animals were treated with extract of A. capitata at doses of 100 and 200 mg/kg. Lipid profiles were observed and compared. Results:Administration of A. capitata caused significant decrease in cholesterol, triglyceride and low density lipoprotein-cholesterol when compared with the control (P<0.05) which was dose dependent. Also, it was observed that high density lipoprotein-cholesterol was significantly increased when compared with the control. Conclusions:This observation suggests that the leaf extract of A. capitata could probably serve as a potential natural product for treatment of hyperlipidaemia.

  5. 7 CFR 318.13-22 - Bananas from Hawaii.

    Science.gov (United States)

    2010-01-01

    ... chapter for the Mediterranean fruit fly (Ceratitis capitata), the melon fruit fly (Bactrocera curcurbitae... requirements in § 305.34 of this chapter for the Mediterranean fruit fly (Ceratitis capitata), the melon...

  6. 7 CFR 319.56-20 - Apples and pears from Australia (including Tasmania) and New Zealand.

    Science.gov (United States)

    2010-01-01

    ... requirements of § 319.56-5 for pest freedom: Mediterranean fruit fly (Ceratitis capitata), the Queensland fruit... fruit fly (Ceratitis capitata), the Queensland fruit fly (Dacus tryoni), Bactrocera jarvisi, and...

  7. 75 FR 5034 - Determination of Pest-Free Areas in the Republic of Chile; Request for Comments

    Science.gov (United States)

    2010-02-01

    ... to recognize additional areas as pest-free areas for Mediterranean fruit fly (Ceratitis capitata) in... country as being free of Ceratitis capitata, Mediterranean fruit fly (Medfly).\\1\\ Specifically,...

  8. 76 FR 56730 - Determination of Pest-Free Areas in Australia; Request for Comments

    Science.gov (United States)

    2011-09-14

    ... recognize additional areas as pest- free areas for Mediterranean fruit fly (Ceratitis capitata) or... being free of Ceratitis capitata, the Mediterranean fruit fly (Medfly), and to recognize other areas...

  9. Wie Nationalismus das Geschlecht macht How Nationalism Creates Gender

    Directory of Open Access Journals (Sweden)

    Ruben Marc Hackler

    2006-07-01

    Full Text Available Heidrun Zettelbauer untersucht in ihrer Dissertation den Zusammenhang von Geschlecht und Nation in den völkischen Diskursen der Habsburgermonarchie zwischen 1880 und 1918. Anhand von Vereinspublikationen weist sie überzeugend nach, wie geschlechtsspezifische Zuschreibungen durch die nationalistische Bewegung befestigt und vorangetrieben worden sind. Gleichzeitig zeigt sie, dass die Bewegung auf die Integration von Frauen angewiesen war, was die Möglichkeit für Frauen bot, sich im Verein politisch zu betätigen und so die bürgerliche Auffassung weiblicher Subjektivität zu unterlaufen.Heidrun Zettelbauer’s dissertation examines the relationship between gender and nation in the peoples’ discourses of the Habsburg monarchy between 1880 and 1918. Using publications from clubs and organizations, she convincingly proves the manner in which gender-specific ascriptions were solidified and perpetuated by the nationalist movement. Concurrently, the movement was dependant upon the integration of women, which gave women the possibility of politically operating within the organization. They thus undermined the bourgeois understanding of female subjectivity.

  10. Eficácia de atrativos alimentares na captura de moscas-das-frutas em pomar de citros

    OpenAIRE

    Raga, Adalton; Machado,Rogério Amaro; Dinardo,Welci; Strikis,Pedro Carlos

    2006-01-01

    As moscas-das-frutas (Diptera: Tephritoidea) são importantes pragas de frutas cítricas no Estado de São Paulo, principalmente Anastrepha fraterculus (Wied.) e Ceratitis capitata (Wied.). O objetivo deste estudo foi medir a eficiência dos atrativos alimentares para monitoramento de moscas-das-frutas, em pomar de citros. O delineamento experimental adotado foi de blocos ao acaso com cinco tratamentos e cinco repetições. O ensaio foi instalado em 30/9/2003 e desenvolvidopor nove semanas. Os atra...

  11. Comparative analysis of development and survival of two Natal fruit fly Ceratitis rosa Karsch (Diptera, Tephritidae) populations from Kenya and South Africa.

    Science.gov (United States)

    Tanga, Chrysantus M; Manrakhan, Aruna; Daneel, John-Henry; Mohamed, Samira A; Fathiya, Khamis; Ekesi, Sunday

    2015-01-01

    Comparative analysis of development and survivorship of two geographically divergent populations of the Natal fruit fly Ceratitis rosa Karsch designated as Ceratitis rosa R1 and Ceratitis rosa R2 from Kenya and South Africa were studied at seven constant temperatures (10, 15, 20, 25, 30, 33, 35 °C). Temperature range for development and survival of both populations was 15-35 °C. The developmental duration was found to significantly decrease with increasing temperature for Ceratitis rosa R1 and Ceratitis rosa R2 from both countries. Survivorship of all the immature stages of Ceratitis rosa R1 and Ceratitis rosa R2 from Kenya was highest over the range of 20-30 °C (87-95%) and lowest at 15 and 35 °C (61-76%). Survivorship of larvae of Ceratitis rosa R1 and Ceratitis rosa R2 from South Africa was lowest at 35 °C (22%) and 33 °C (0.33%), respectively. Results from temperature summation models showed that Ceratitis rosa R2 (egg, larva and pupa) from both countries were better adapted to low temperatures than R1, based on lower developmental threshold. Minimum larval temperature threshold for Kenyan populations were 11.27 °C and 6.34 °C (R1 and R2, respectively) compared to 8.99 °C and 7.74 °C (R1 and R2, respectively) for the South African populations. Total degree-day (DD) accumulation for the Kenyan populations were estimated at 302.75 (Ceratitis rosa R1) and 413.53 (Ceratitis rosa R2) compared to 287.35 (Ceratitis rosa R1) and 344.3 (Ceratitis rosa R2) for the South African populations. These results demonstrate that Ceratitis rosa R1 and Ceratitis rosa R2 from both countries were physiologically distinct in their response to different temperature regimes and support the existence of two genetically distinct populations of Ceratitis rosa. It also suggests the need for taxonomic revision of Ceratitis rosa, however, additional information on morphological characterization of Ceratitis rosa R1 and Ceratitis rosa R2 is needed.

  12. 76 FR 51934 - Determination of Pest-Free Areas in Mendoza Province, Argentina; Request for Comments

    Science.gov (United States)

    2011-08-19

    ... of that country as being free of Ceratitis capitata, Mediterranean fruit fly (Medfly). Specifically... recognize additional areas as pest-free areas for Mediterranean fruit fly (Ceratitis capitata) in Argentina... Ceratitis capitata, the Mendoza province of Argentina also be recognized as free of A. fraterculus. \\2\\...

  13. 78 FR 25620 - Importation of Female Squash Flowers From Israel Into the Continental United States

    Science.gov (United States)

    2013-05-02

    ... through the importation of immature fruit attached to female squash flowers. These are Ceratitis capitata... are designed to prevent the introduction of the following quarantine pests: Ceratitis capitata, Dacus... inspected and found free of Ceratitis capitata, Dacus ciliatus, Helicoverpa armigera, and...

  14. Enthalpy of formation of (In, Gd)-doped barium cerate

    Energy Technology Data Exchange (ETDEWEB)

    Matskevich, N.I., E-mail: nata.matskevich@yandex.ru [Nikolaev Institute of Inorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk (Russian Federation); Karlsruhe Institute of Technology, Institute of Solid State Physics, D-76334 Karlsruhe (Germany); Wolf, Th. [Karlsruhe Institute of Technology, Institute of Solid State Physics, D-76334 Karlsruhe (Germany); Adelmann, P.; Semerikova, A.N.; Anyfrieva, O.I. [Nikolaev Institute of Inorganic Chemistry, Siberian Branch of the Russian Academy of Sciences, 630090 Novosibirsk (Russian Federation)

    2015-09-10

    Highlights: • BaCe{sub 0.7}Gd{sub 0.2}In{sub 0.1}O{sub 2.85} was prepared by solid-state reaction. • The standard formation enthalpy was determined. • The stabilization energy (Δ{sub st}H°) was calculated. • Δ{sub st}H° of BaCe{sub 0.7}Gd{sub 0.2}In{sub 0.1}O{sub 2.85} is higher than BaCe{sub 0.7}Nd{sub 0.2}In{sub 0.1}O{sub 2.85} and BaCeO{sub 3}. - Abstract: Solution enthalpies of barium cerate doped by gadolinium and indium and a mixture of BaCl{sub 2} + 0.7CeCl{sub 3} + 2GdCl{sub 3} + 0.1InCl{sub 3} have been measured in 1 mol dm{sup −3} HCl with 0.1 mol dm{sup −3} KI. For the first time the standard molar formation enthalpy of BaCe{sub 0.7}Gd{sub 0.2}In{sub 0.1}O{sub 2.85} has been determined by solution calorimetry as follows: Δ{sub f}H° (298.15 K) = −1615.84 ± 9.01 kJ mol{sup −1}. The stabilization energy for above-mentioned compound has been calculated as well. It has been shown that barium cerate doped gadolinium and indium has higher stabilization energy than BaCe{sub 0.7}Nd{sub 0.2}In{sub 0.1}O{sub 2.85} and BaCeO{sub 3}. The reaction enthalpy with CO{sub 2} interaction has been calculated for BaCe{sub 0.7}Gd{sub 0.2}In{sub 0.1}O{sub 2.85}.

  15. The preparation of the rice coral Montipora capitata nubbins for application in coral-reef ecotoxicology.

    Science.gov (United States)

    Vijayavel, K; Richmond, R H

    2012-04-01

    Securing adequate and appropriate source material for coral-reef ecotoxicology studies is a significant impediment to conducting various experiments supporting the goal of conserving coral-reef ecosystems. Collecting colonies from wild stocks may be counter to protecting coral reef populations. To address this issue the rice coral Montipora capitata was used to generate sufficient genetically identical nubbins for research purposes. Growth and survival rates of these laboratory-prepared M. capitata nubbins were studied over a period of 90 days. The resulting data support the conclusion that the laboratory-prepared M. capitata nubbins showed successful growth and survival rates and are the best solution to solve the source material issue for lab experimentation. This paper describes the laboratory method used for the preparation and maintenance of these M. capitata nubbins and discusses the benefits and difficulties of using these nubbins in ecotoxicity studies.

  16. Isolation and characterization of microsatellite loci in tropical forage Stylosanthes capitata Vogel.

    Science.gov (United States)

    Santos, M O; Sassaki, R P; Chiari, L; Resende, R M S; DE Souza, A P

    2009-01-01

    Stylosanthes capitata is an important tropical pasture legume. Knowledge of genetic diversity and structure of S. capitata populations is of great importance for the conservation and germplasm management of this species. Thus, eight microsatellite markers were developed from an S. capitata-enriched library. They were characterized in 20 accessions from the germplasm collection of the Empresa Brasileira de Pesquisa Agropecuária (Embrapa). The observed and expected heterozygosities ranged from 0.16 to 0.85 and from 0.40 to 0.85, respectively. These microsatellites are the first set of molecular markers from this species and will contribute towards studies of genetic diversity, conservation and breeding of S. capitata.

  17. Captures of wild Ceratitis capitata Bactrocera dorsalis and Bactrocera cucurbitae (Diptera: Tephritidae) in traps with improved multi-lure TMR-Dispensers weathered in California

    Science.gov (United States)

    During 2012-2013 two “attract and kill” systems were weathered in California as potential detection and male annihilation treatments (MAT). Solid Mallet TMR (trimedlure [TML], methyl eugenol [ME], raspberry ketone [RK]) wafers impregnated with DDVP (2, 2-dichlorovinyl dimethyl phosphate) insecticide...

  18. Ammonium acetate enhances the attractiveness of a variety of protein-based baits to female Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae)

    Science.gov (United States)

    Ammonia and its derivatives are used largely by female fruit 32 flies (Diptera: Tephritidae) as volatile cues to locate protein-rich food needed to produce their eggs. This need for external protein sources has led to the development of behaviorally-based control strategies such a food-based lures a...

  19. The whole genome sequence of the Mediterranean fruit fly, Ceratitis capitata (Weidmann): a highly invasive and destructive pest of fruits and vegetables throughout the world

    Science.gov (United States)

    The Mediterranean fruit fly is one of the most destructive agricultural pests throughout the world due to its broad host plant range that includes more than 260 different fruits, flowers, vegetables, and nuts. Host preferences vary in different regions of the world, which can be associated with its ...

  20. 78 FR 25692 - Notice of Decision To Authorize the Importation of Fresh Barhi Dates From Israel

    Science.gov (United States)

    2013-05-02

    ... advising the public of our decision to add a treatment schedule for Ceratitis capitata in Barhi dates to... from Israel was treating the dates with cold treatment for Ceratitis capitata. Because no such cold... mitigation for C. capitata, and described a cold treatment schedule for C. capitata in Barhi dates that...

  1. Cuticular hydrocarbons corroborate the distinction between lowland and highland Natal fruit fly (Tephritidae, Ceratitis rosa) populations

    Science.gov (United States)

    Vaníčková, Lucie; Břízová, Radka; Pompeiano, Antonio; Ekesi, Sunday; Meyer, Marc De

    2015-01-01

    Abstract The cuticular hydrocarbons (CHs) and morphology of two Ceratitis rosa Karsch (Diptera: Tephritidae) populations, putatively belonging to two cryptic taxa, were analysed. The chemical profiles were characterised by two-dimensional gas chromatography with mass spectrometric detection. CHs of Ceratitis rosa that originated from the lowlands and highlands of Kenya comprised of n-alkanes, monomethylalkanes, dimethylalkanes and unsaturated hydrocarbons in the range of the carbon backbone from C14 to C37. Hydrocarbons containing C29, C31, C33 and C35 carbon atoms predominated in these two populations. 2-Methyltriacontane was the predominant compound in both populations. Quantitative differences in the distribution of hydrocarbons of different chain lengths, mainly the C22, C32, C33 and C34 compounds of these two populations, were observed despite indistinct qualitative differences in these hydrocarbons. Morphological analyses of male legs confirmed that the flies belong to different morphotypes of Ceratitis rosa previously labelled as R1 and R2 for lowland and highland populations, respectively. A statistical analysis of the CH compositions of the putative R1 and R2 species showed distinct interspecific identities, with several CHs specific for each of the lowland and highland populations. This study supports a hypothesis that the taxon Ceratitis rosa consists of at least two biological species. PMID:26798275

  2. Liebe, Neugier, Spiel - Wie kommt das Neue in die Welt? Systemische und neurobiologische Betrachtungen

    OpenAIRE

    2011-01-01

    Wie für viele andere, so hat Gerald Hüther auch für mich das Tor zu den Neurowissenschat en aufgestoßen, Neugier und Faszination ob der vielen Entdeckungen und Entwicklungen geweckt. Einige seiner Bücher (Hüther, 1998, 2001b, 2004) haben eigene Bilder aktiviert: Vor und im Studium arbeitete ich viele Jahre im Straßenbau. Vom Vermessungswesen über die Erdarbeiten bis zur Teerkolonne hatte ich einige Stufen des Straßenbaus kennen gelernt. Mich faszinierte, wie eine neue Straße entstand: Ich er...

  3. Deflation in Südeuropa: Fluch oder Segen? Wie sollte die EZB reagieren?

    OpenAIRE

    Alfons J. Weichenrieder; Adalbert Winkler; Anja Rossen; Olaf Schlotmann

    2014-01-01

    Seit mehr als einem Jahr ist der Preisanstieg im Euroraum im Trend rückläufig. Angesichts der geringen Inflationsrate wird über ein mögliches Abrutschen in eine Deflation diskutiert. Wie groß ist diese Gefahr und wie sollte die EZB reagieren? Das wahrscheinlichere Szenario der nächsten Monate besteht nach Ansicht von Alfons J. Weichenrieder, Goethe-Universität Frankfurt, eher in einer sogenannten »Lowflation« – zu wenig Inflation – statt einer Deflation. Doch auch schon die Lowflation berge P...

  4. Caracterização química da amêndoa de coquinho-azedo (Butia capitata var capitata Chemical characterization of nut of Butia capitata var capitata

    Directory of Open Access Journals (Sweden)

    Juliana Pereira Faria

    2008-06-01

    Full Text Available A palmeira Butia capitata var capitata produz o coquinho-azedo, fruto de odor agradável e penetrante, cuja polpa é aproveitada para a produção de um suco saboroso na região norte de Minas Gerais. As sementes apresentam uma amêndoa cuja exploração ainda é bastante limitada. O objetivo deste trabalho foi determinar a composição da amêndoa do coquinho-azedo, visando a avaliar suas possibilidades de uso. Na amêndoa, a composição química (umidade, proteínas, lipídios, cinzas e fibras foi determinada por métodos gravimétricos. Os teores dos principais minerais foram avaliados por espectrofotometria de emissão atômica com fonte de plasma indutivo, e a composição dos ácidos graxos presentes na gordura da semente foi determinada por cromatografia a gás. A amêndoa do coquinho-azedo apresentou 9,9 % de umidade e 57,8 % de lipídios totais, 25,8 % de fibra detergente neutro, 17,6 % de fibra detergente ácido e 1,6 % de cinzas em base seca. A gordura extraída da amêndoa de coquinho-azedo apresentou elevados teores de ácido láurico (42,1 %, que foi seguido pelo ácido oléico (16,9 %. Predominaram os ácidos graxos saturados (78,9 %, principalmente os de cadeia média (C6-C12.The Butia capitata palm tree produce a very aromatic fruit named "coquinho-azedo", which freeze pulp is frequently used to produce agreeable juice in north of Minas Gerais, Brazil. "Coquinho-azedo" kernel contains a nut that is not frequently explored. The purpose of the present study was the chemical characterization of "coquinho-azedo" nut to evaluate the possibilities of its use. The moisture, lipids, ash and fiber contents were determined by gravimetric methods. The mineral profile was characterized by inductively coupled plasma spectrophotometer and the fatty acids profile was characterized by gas chromatography. The coquinho-azedo nut presented 9,9 % of moisture, 57,8 % of total lipid, 25,8 % of neutral detergent fiber, 17,6 % of acid detergent fiber

  5. ESPÉCIES E FLUTUAÇÃO POPULACIONAL DE MOSCAS-DAS-FRUTAS EM UM POMAR COMERCIAL DE MANGUEIRA, NO LITORAL DO ESTADO DO CEARÁ

    Directory of Open Access Journals (Sweden)

    Joseph Jonathan Dantas de Oliveira

    2009-01-01

    Full Text Available The objective of this research was to know the species and the population fluctuation of fruit flies (Diptera: Tephritidae in a commercial mango (Mangifera indica L. orchard in the coast of Ceará State. The study was developed from July of 2005 to July of 2007, in the municipality of Beberibe (CE. The capture of the fruit flies was performed using McPhail traps with 5% corn protein hydrolyzed solution as attractant. Weekly, the captured insects were sorted, the fruit flies were maintained in 70% alcohol solution and subsequently identified. The population fluctuation was estimated using the FTD (Fly/Trap/Day index. During the research, six fruit flies species were captured: Anastrepha obliqua (Macquart (63%, A. zenildae Zucchi (7%, A. sororcula Zucchi (5%, A. fraterculus (Wied. (2%, A. distincta Greene (2% and Ceratitis capitata (Wied. (21%. The Anastrepha spp. and C. capitata population peaked was between April and July, in both years of study.

  6. De status van Uber – Wie betaalt er aan het eind van de rit?

    NARCIS (Netherlands)

    de Vries, Anne

    2016-01-01

    Taxi-app Uber biedt consumenten een snelle en makkelijke manier om taxi’s te bestellen. Er kleven voor de consument echter ook juridische risico’s aan het gebruik van deze app. Dit artikel bespreekt de vraag wie de consument kan aanspreken als de taxidienst niet naar behoren wordt uitgevoerd: Uber o

  7. Methods to Break Seed Dormancy of Rhynchosia capitata, a Summer Annual Weed Métodos para Romper la Dormancia de Rhynchosia capitata, una Maleza Anual de Verano

    Directory of Open Access Journals (Sweden)

    Hafiz Haider Ali

    2011-09-01

    Full Text Available Dormancy of weed seeds is a significant feature contributing to their survival rate since it helps the weeds to avoid herbicides and other weeding practices along with unfavorable environmental conditions. We investigated the effects of different dormancy breaking treatments on the germination of Rhynchosia capitata, a common summer annual weed, which is emerging as a weed threat in Pakistan. Seeds were soaked in thiourea, KNO3, HCl, HNO3, and H2SO4, and they were also mechanically scarified (sandpaper. Results indicated that R. capitata seeds show signs of physical dormancy that is mainly due to the impermeability of their coat. Mechanical scarification and acid scarification (soaking of seeds in H2SO4 for 60 and 80 min and in HCl for 12 and 15 h were very efficient in breaking dormancy and promoting germination. Seed soaking in HNO3 for 1 to 5 d showed little effect whereas various concentrations of thiourea and KNO3 were ineffective in breaking R. capitata seed dormancy.La dormancia seminal de las malezas es un rasgo significativo contribuyente a su tasa de supervivencia, puesto que ayuda a las malezas a evitar herbicidas y otras prácticas de desmalezado junto con condiciones ambientales desfavorables. Investigamos los efectos de diferentes tratamientos para romper dormancia sobre la germinación de Rhynchosia capitata, una maleza anual estival común en Paquistán. Las semillas se sumergieron en tiourea, KNO3, HCl, HNO3 y H2SO4 y además fueron escarificadas mecánicamente (papel lija. Los resultados indicaron que las semillas de R. capitata muestran signos de dormancia física principalmente debido a impermeabilidad de su cubierta. Escarificación mecánica y ácida (inmersión de semillas en H2SO4 por 60 y 80 min y en HCl por 12 y 15 h fueron muy eficientes para romper dormancia y promover germinación. Las semillas sumergidas en HNO3 por 1 a 5 días mostraron poco efecto, mientras diversas concentraciones de tiourea y KNO3 fueron

  8. Sterile insect technique: new technology to control fruit flies, Ceratitis capitata, in the Lower Basin of the Sao Francisco Valley; Tecnica do inseto esteril: nova tecnologia para combater a mosca-das-frutas, Ceratitis capitata, no Submedio do Vale do Sao Francisco

    Energy Technology Data Exchange (ETDEWEB)

    Paranhos, Beatriz Aguiar Jordao; Barbosa, Flavia Rabelo [Embrapa Semi-Arido, Petrolina, PE (Brazil); Nascimento, Antonio Souza do [Embrapa Mandioca e Fruticultura, Cruz das Almas, BA (Brazil); Viana, Rodrigo; Malavasi, Aldo [Moscamed Brasil, Juazeiro, BA (Brazil); Sampaio, Raimundo [Agencia Estadual de Defesa Agropecuaria da Bahia (ADAB-BA), Salvador, BA (Brazil); Walder, Julio Marcos Melges [Centro de Energia Nuclear na Agricultura (CENA/USP), Piracicaba, SP (Brazil)

    2008-12-15

    The SIT is the creation, on a large scale, the insect-pest to be controlled and weekly release of these insects sterilized in the field.The sterile insects copulate with the wild, but do not generate descendants. The basic premises for the use of SIT in insect control are: the reproduction is through sexual intercourse, the female copulate preferably only once there is ease of creation of the plague in industrial scale in artificial diet. The efficiency of the SIT may be greater when only the males are released in the field because they increase the probability of copulating with wild females only, with reductions in the cost of production and release. In the case of fruit-flies, sterile females continue doing puncture in the fruits, which decreases the quality for export. To be able to release only males in the field, in 1980s, was developed a mutant strain, whose females emerge from pupae white, thus being able to discard the white ones, keeping the pupae Brown for the release of sterile males. Ten years after, to save on the industrial scale production system, was obtained a mutant whose females possess lethal temperature sensitivity of 34 deg C, still in the embryo stage. Then the eggs are placed on artificial diet, and when they arrive at the pupa stage, they are all brown and males. Forty-eight to 24 hours before the emergence of adults, the pupae are painted with fluorescent powder paint, bagged and irradiated with gamma radiation of 95Gy of Co-60 or X-ray. As soon as the males emerge, are marked with fluorescent ink and when they reach 3 to 5 days old, are released into the field. Thus, when monitoring is done in Jackson traps in the field, it is possible to distinguish wild male sterile under black light or epifluorescence microscope with males, because the sterile are fluorescent. On application of the SIT to Moscamed, sterile males are released in the field must display good dispersibility, good survival and good sexual performance. The efficiency and effectiveness of the use of the SIT depend on the success of the sterile males in competition with wild males for copulating of females. According to FAO / IAEA / USDA (2003 ), so that the SIT is technically feasible in controlling Moscamed, it must occur in field conditions, at least 20 % of matings between sterile males and wild females tsl. One of the ways to increase the efficiency of SIT is releasing a nine to one hundred times the population of sterile males compared to the wild population in this field, it increases the likelihood of wild females are copulated by sterile males. Understanding the behaviors and strategies used by the wild and sterile males approach the females for copulating has great relevance to the SIT programs, since determining the acceptance and response of wild females. The strategy in copulating fruit-flies consists of: a) clustering of males at the bottom of foliage of host or non-host plants; b) issuance of the sex pheromone; c) call through the beating of the wings; d) arrival of the female, which is the male face to face; e) both touch with antennas; f) males jump about the females and initiate copulating, and the female may or may not accept the male g) end copulation.

  9. Wer im Treibhaus sitzt wie wir der Klima- und Energiefalle entkommen

    CERN Document Server

    Kleinknecht, Konrad

    2007-01-01

    Deutschland leistet sich eine verlogene Debatte um die Zukunftsfrage schlechthin: Wie entkommen wir der Energie- und Klimafalle? Mit Parolen aus den Siebzigerjahren (»Atom­kraft – nein danke!«) und Vogel-Strauß-Politik verspielen wir unsere Zukunft als Industrienation. Konrad Kleinknecht zeigt, wie alarmierend die Situation ist. Schon bald wird die Klimaänderung dazu führen, dass Millionen Menschen nach Europa kommen, weil ihre Lebensräume unbewohnbar geworden sind, schon bald wird Energie, der Lebenssaft unserer Zivilisation, nicht mehr ausreichend verfügbar oder bezahlbar sein. Als Physiker kann Kleinknecht klar nachweisen, dass der bisherige Energiekurs, das Setzen auf fossile Energien, in den Untergang führt.

  10. Strongyloides ferreirai sp.n. (Nematoda, Rhabdiasoidea parasito do roedor Kerodon rupestris (Wied. no Brasil

    Directory of Open Access Journals (Sweden)

    H. de Oliveira Rodrigues

    1985-12-01

    Full Text Available É descrita uma nova espécie de nematóide do gênero Strongyloides Grassi, 1879, Strongyloides ferreirai sp.n. parasita do instestino delgado de Kerodon rupestris (Wied. proveniente de Floriano Peixoto, Estado do Piauí. Esta é a primeira referência deste gênero parasitando roedor caviideo no Brasil.Strongyloides ferreirai n.sp. is described and compared to the closely related species S. ratti, S. venezuelensis and S. myopotami, parasites of rodents in Brazil. The description is based on parasitic females, recovered from the small intestine of Kerodon rupestris (Wied. (Caviidae captured in Floriano Peixoto, Piauí State, Brazil.

  11. Digitale Ungleichheiten – theoretisch … wie praktisch! Alltagsbetrachtungen zu einer postmodernen, sozialen Herausforderung des 21. Jahrhunderts

    Directory of Open Access Journals (Sweden)

    Karlheinz Benke

    2009-10-01

    Full Text Available Selbst wenn es aus vielfacher Perspektive betrachtet auf den ersten Blick seltsam einfach klingen mag: es gibt sie nach wie vor – die Ungleichheit und Spaltung zwischen arm und reich, zwischen jung und alt, zwischen Ortansässigen und Zugewanderten, zwischen Stadt und Land, zwischen krank und gesund, allerdings im Kontext mit den Neuen Medien. Soziale Ungleichheiten übertragen sich in Ableitung der Wissenskluft in den virtuellen Raum hinein und zeigen sich ‚dort’ in verschiedensten Facetten. Auch wenn sie nach wie vor von der Öffentlichkeit, der Politik, von Ämtern und Institutionen nicht als Diskrepanz oder Disproportionalität wahrgenommen werden. Ein Zugang zu Neuen Medien, zum Netz wird in unserer Gesellschaft stillschweigend vorausgesetzt. Ein fataler Trugschluss, der kaum wirklich eine Chance auf Veränderung lässt? UnGleich wird UnGleicher?

  12. Phytochemical and biological studies of Butia capitata Becc. leaves cultivated in Egypt

    Directory of Open Access Journals (Sweden)

    Nagwa Mohamed Ammar

    2014-06-01

    Conclusions: B. capitata leaf extracts were shown to possess variable antioxidant effect, the most promising was methanol extract. Both polar and non polar extracts were proved to have anti-inflammatory activity, the non polar extract was superior in this respect. The bioactivity of the extracts was ascribed to the presence of flavonoids, sterols and α-tocopherol.

  13. Phytochemical and biological studies of Butia capitata Becc. leaves cultivated in Egypt

    Institute of Scientific and Technical Information of China (English)

    Nagwa Mohamed Ammar; Mohammed Said Hefnawy; Sahar Youssef Al-Okbi; Doha Abdou Mohamed; Nabil Khamis El-Sayed; Amira Ahmed El-Anssary; Tom Mabry

    2014-01-01

    Objective: To study the antioxidant and anti-inflammatory activity of Butia capitata (B. capitata) leaf extracts along with phytochemical analysis of the proposed bioactive constituents.Methods:organic solvents and screened for their anti-inflammatory activities in tested animals and in-vitro antioxidant effect. An extensive phytochemical investigation of the bioactive extracts through paper chromatography, thin layer chromatography, column chromatography, gas-liquid chromatography (GLC), high pressure liquid chromatography and spectral analysis. GC-Mass, ultraviolet, hydrogen and carbon nuclear magnetic resonance, electron ionization-mass spectrometry, heteronuclear multiple bond correlation and heteronuclear multiple quantum correlation were carried out.Results:Results showed that different extracts possess promising antioxidant effect and Different successive extracts of B. capitata Becc. leaves were prepared with selective significant anti-inflammatory activity with variable degrees. The results of the phytochemical investigation of the bioactive extracts revealed the presence of volatile substances, lipoidal matter, α-tocopherol, free sugars, polysaccharides and flavonoidal compounds.Conclusions: B. capitata leaf extracts were shown to possess variable antioxidant effect, the most promising was methanol extract. Both polar and non polar extracts were proved to have anti-inflammatory activity, the non polar extract was superior in this respect. The bioactivity of the extracts was ascribed to the presence of flavonoids, sterols and α-tocopherol.

  14. Invasive infection due to Saprochaete capitata in a young patient with hematological malignancies

    Science.gov (United States)

    Parahym, Ana Maria Rabelo de Carvalho; Rolim, Pedro José; da Silva, Carolina Maria; Domingos, Igor de Farias; Gonçalves, Sarah Santos; Leite, Edinalva Pereira; de Morais, Vera Lúcia Lins; Macêdo, Danielle Patrícia Cerqueira; de Lima, Reginaldo Gonçalves; Neves, Rejane Pereira

    2015-01-01

    We report a case of invasive infection due to Saprochaete capitata in a patient with hematological malignancies after chemotherapy treatment and empiric antifungal therapy with caspofungin. Although severely immunocompromised the patient survived been treated with amphotericin B lipid complex associated with voriconazole. PMID:26273269

  15. Review: Larissa Förster, Postkoloniale Erinnerungslandschaften. Wie Deutsche und Herero in Namibia des Kriegs von 1904 gedenken (2010 Buchbesprechung: Larissa Förster, Postkoloniale Erinnerungslandschaften. Wie Deutsche und Herero in Namibia des Kriegs von 1904 gedenken (2010

    Directory of Open Access Journals (Sweden)

    Reinhart Kößler

    2010-01-01

    Full Text Available Review of the Monograph: Larissa Förster (2010, Postkoloniale Erinnerungslandschaften. Wie Deutsche und Herero in Namibia des Kriegs von 1904 gedenken, Frankfurt am Main & New York: Campus, ISBN 978-3-593-39160-1, 391 pages. Besprechung der Monographie: Larissa Förster (2010, Postkoloniale Erinnerungslandschaften. Wie Deutsche und Herero in Namibia des Kriegs von 1904 gedenken, Frankfurt am Main & New York: Campus, ISBN 978-3-593-39160-1, 391 Seiten.

  16. Generation and characterization of Brassica rapa ssp. pekinensis – B. oleracea var. capitata monosomic and disomic alien addition lines

    Indian Academy of Sciences (India)

    Ai xia Gu; Shu Xing Shen; Yan Hua Wang; Jian Jun Zhao; Shu Xin Xuan; Xue Ping Chen; Xiao Feng Li; Shuang Xia Luo; Yu Jing Zhao

    2015-09-01

    Five monosomic alien addition lines (MAALs) of Brassica rapa ssp. pekinensis – B. oleracea var. capitata were obtained by hybridization and backcrossing between B. rapa ssp. pekinensis (female parent) and B. oleracea var. capitata. The alien linkage groups were identified using 42 B. oleracea var. capitata linkage group-specific markers as B. oleracea linkage groups C2, C3, C6, C7 and C8. Based on the chromosomal karyotype of root tip cells, these five MAALs added individual chromosomes from B. oleracea var. capitata: chr 1 (the longest), chr 2 or 3, chr 5 (small locus of 25S rDNA), chr 7 (satellite-carrying) and chr 9 (the shortest). Five disomic alien addition lines were then generated by selfing their corresponding MAALs.

  17. 78 FR 6227 - Importation of Fresh Apricots From Continental Spain

    Science.gov (United States)

    2013-01-30

    ... fly (Medfly), Ceratitis capitata Wiedemann, The plum fruit moth, Cydia funebrana (Treitschke), Leaf...: Apiognomonia erythrostoma (Pers.), a brown rot fungus; Ceratitis capitata Wiedemann, the Mediterranean fruit... fruit would be cut open to inspect for the internal pest C. capitata. If any of these quarantine...

  18. 78 FR 50023 - Importation of Fresh Oranges and Tangerines From Egypt Into the United States

    Science.gov (United States)

    2013-08-16

    ... fly (Bactrocera zonata) and Mediterranean fruit fly (Ceratitis capitata) in oranges and tangerines... effective against B. zonata in oranges and tangerines. In addition to B. zonata, Ceratitis capitata (Medfly... for C. capitata and B. zonata; and The oranges and tangerines must be accompanied by a...

  19. 78 FR 1825 - Importation of Fresh Barhi Dates From Israel Into the United States

    Science.gov (United States)

    2013-01-09

    ... treatment evaluation document that describes a new treatment schedule for Ceratitis capitata in Barhi dates... Ceratitis capitata; and The dates must be accompanied by a phytosanitary certificate issued by the national... schedule for C. capitata in Barhi variety dates. In accordance with Sec. 305.3(a)(1), we are...

  20. A Sterile Insect Technique (S.I.T.) Study Project to control Medfly in a Southern region of Italy

    Energy Technology Data Exchange (ETDEWEB)

    Tata, A.; Cirio, U.; Balducci, R. [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dipt. Innovazione

    1997-12-01

    Since 1967 the National Agency for New Technology, Energy and the Environment (ENEA) namely the main Italian governmental technological research organization, is carrying out R and D programmes and demonstrative projects aimed to set up S.I.T. (Sterile Insect Technique) processes. In the framework of a world-wide growing interest concerning pest control technology, the National Agency for New Technology, Energy and the Environment (ENEA) developed a very large industrial project aimed to control Medfly (Ceratitis capitata Wied.) with reference to fruit crops situation in Sicily region (Southern of Italy) through the production and spreading of over 250 million sterile flies per week.

  1. SYSTEMATIC STUDIES IN TURNERA (TURNERACEAE II. SERIES ANNULARES, CAPITATAE, PAPILLIFERAE AND MICROPHYLLAE ESTUDIOS SISTEMÁTICOS EN TURNERA (TURNERACEAE II. SERIES ANNULARES, CAPITATAE, MICROPHYLEAE Y PAPILLIFERAE

    Directory of Open Access Journals (Sweden)

    María Mercedes Arbo

    2010-11-01

    Full Text Available

    Turnera L. includes around 100 American species and two African ones, arranged in nine series. In this paper, the following four series embracing 21 species are revised: Annulares, Capitatae, Microphyllae and Papilliferae. Six new taxa are described: Thatschbachii, Thatschbachii var. miniata, Tmaracasana,and T.princeps of the seriesCapitatae, Tasymmetrica of the series Microphyllae, and Tcaatingana of the series Papilliferae. The species of these four series share the common features of simple and glandular hairs, generally well-developed floral peduncle, absent pedicel, linear or elliptic prophylls, generally warty or granulate fruit, and a floral tube, appendicular in nature, in that it is the product of the fusion of the lower part of the calyx and corolla, with the staminal filaments adnate only at the base. In the series Annulares,
    Microphyllae and Papilliferae the leaves and young stems are fragrant, the scent being produced by the glandular hairs, and the floral peduncle is shortly adnate to the petiole. Three
    series, Annulares, Capitatae and Microphyllae, have leaves with generally well developed stipules and a disjunct geographical distribution. The series Annulares and Capitatae have nectaries and villous or hirsute styles, while Microphyllae and Papilliferae lack nectaries and their styles are glabrous or somewhat pilose. In some species of Microphyllae the staminal filaments are joined at the base, forming an annular structure 0,05-0,50 mm high. The seeds are peculiar in Annulares, being very short, with a central, prominent and slightly concave chalaza. In the series Capitatae, the flowers are gathered in short, capitate inflorescences, the length of the peduncles being variable or sometimes wanting, and the seeds are obovoid, reticulate or striate. The leaves of Microphyllae are small, with the stipules adnate to the foliar
    base; setiform glandular hairs, similar to those of

  2. Haftung und Compliance: Wie beeinflusst eine CMS-Zertifizierung den Sorgfaltsmaßstab?

    Directory of Open Access Journals (Sweden)

    Mona Philomena Ladler

    2016-05-01

    Full Text Available Eine ungenügende Compliance-Organisation stellt eine Sorgfaltspflichtverletzung der Geschäftsleiter dar. CMS-Standards, wie die Norm ISO 19600, bieten Hilfestellungen zur Ausgestaltung einer Compliance-Organisation und können daher das Haftungsrisiko der Geschäftsleitung reduzieren. In diesem Beitrag wird der Frage nach der Rechtsqualität von CMS-Standards nachgegangen. Es werden die Haftungsfolgen einer Zertifizierung einer Compliance-Organisation auf Basis von CMS-Standards untersucht und analysiert, ob die ordentliche Sorgfalt die Errichtung einer den CMS-Standards entsprechenden Compliance-Organisation gebieten kann.

  3. Wie aus dem Nichts etwas entsteht Gespräche über Antimaterie

    CERN Document Server

    AUTHOR|(CDS)2051122

    2017-01-01

    Antimaterie beschäftigt nicht nur Physiker, sondern auch die Phantasie von Science fiction-Autoren, Star Trek-Fans und Laien. Aber was ist eigentlich Antimaterie, und wie stellt man sie her? Kann man sie speichern? Wird sie bald eine Rolle im Alltag spielen? Und warum verdanken wir unsere Existenz – und die unseres Universums – einem winzigen Unterschied zwischen Materie und Antimaterie? Rolf Landua, einer der weltweit führenden Experten auf diesem Gebiet, beantwortet in seinem neuen Buch anschaulich diese und andere Fragen.

  4. Status of Early 19th-Century Names Authored in Parallel by Wied and Schinz for South American Reptiles and Amphibians, with Designations of Three Nomina Protecta

    OpenAIRE

    2011-01-01

    Prince Maximilian zu Wied's great exploration of coastal Brazil in 1815-1817 resulted in important collections of reptiles, amphibians, birds, and mammals, many of which were new species later described by Wied himself The bulk of his collection was purchased for the American Museum of Natural History in 1869, although many ""type specimens"" had disappeared earlier. Wied carefully identified his localities but did not designate type specimens or type localities, which are taxonomic concepts ...

  5. Inter-specific coral chimerism: Genetically distinct multicellular structures associated with tissue loss in Montipora capitata

    Science.gov (United States)

    Work, Thierry M.; Forsman, Zac H.; Szabo, Zoltan; Lewis, Teresa D.; Aeby, Greta S.; Toonen, Robert J.

    2011-01-01

    Montipora white syndrome (MWS) results in tissue-loss that is often lethal to Montipora capitata, a major reef building coral that is abundant and dominant in the Hawai'ian Archipelago. Within some MWS-affected colonies in Kane'ohe Bay, Oahu, Hawai'i, we saw unusual motile multicellular structures within gastrovascular canals (hereafter referred to as invasive gastrovascular multicellular structure-IGMS) that were associated with thinning and fragmentation of the basal body wall. IGMS were in significantly greater densities in coral fragments manifesting tissue-loss compared to paired normal fragments. Mesenterial filaments from these colonies yielded typical M. capitata mitochondrial haplotypes (CO1, CR), while IGMS from the same colony consistently yielded distinct haplotypes previously only found in a different Montipora species (Montipora flabellata). Protein profiles showed consistent differences between paired mesenterial filaments and IGMS from the same colonies as did seven microsatellite loci that also exhibited an excess of alleles per locus inconsistent with a single diploid organism. We hypothesize that IGMS are a parasitic cellular lineage resulting from the chimeric fusion between M. capitata and M. flabellata larvae followed by morphological reabsorption of M. flabellata and subsequent formation of cell-lineage parasites. We term this disease Montiporaiasis. Although intra-specific chimerism is common in colonial animals, this is the first suspected inter-specific example and the first associated with tissue loss.

  6. Inter-specific coral chimerism: genetically distinct multicellular structures associated with tissue loss in Montipora capitata.

    Directory of Open Access Journals (Sweden)

    Thierry M Work

    Full Text Available Montipora white syndrome (MWS results in tissue-loss that is often lethal to Montipora capitata, a major reef building coral that is abundant and dominant in the Hawai'ian Archipelago. Within some MWS-affected colonies in Kane'ohe Bay, Oahu, Hawai'i, we saw unusual motile multicellular structures within gastrovascular canals (hereafter referred to as invasive gastrovascular multicellular structure-IGMS that were associated with thinning and fragmentation of the basal body wall. IGMS were in significantly greater densities in coral fragments manifesting tissue-loss compared to paired normal fragments. Mesenterial filaments from these colonies yielded typical M. capitata mitochondrial haplotypes (CO1, CR, while IGMS from the same colony consistently yielded distinct haplotypes previously only found in a different Montipora species (Montipora flabellata. Protein profiles showed consistent differences between paired mesenterial filaments and IGMS from the same colonies as did seven microsatellite loci that also exhibited an excess of alleles per locus inconsistent with a single diploid organism. We hypothesize that IGMS are a parasitic cellular lineage resulting from the chimeric fusion between M. capitata and M. flabellata larvae followed by morphological reabsorption of M. flabellata and subsequent formation of cell-lineage parasites. We term this disease Montiporaiasis. Although intra-specific chimerism is common in colonial animals, this is the first suspected inter-specific example and the first associated with tissue loss.

  7. Wie man Elementarteilchen entdeckt vom Zyklotron zum LHC : ein Streifzug durch die Welt der Teilchenbeschleuniger

    CERN Document Server

    Freytag, Carl

    2016-01-01

    Dieses Buch erklärt die physikalischen Grundlagen und die Technologien der Elementarteilchenforschung und beschreibt allgemeinverständlich Teilchenbeschleuniger und -detektoren sowie ihr Zusammenspiel. An einigen Meilensteinen der Forschung – von der Erzeugung von Transuranen über die Entdeckung exotischer Mesonen bis zum Higgs-Boson – zeigen die Autoren den Weg von der Theorie über das Experiment zum Forschungsergebnis auf. Gravitonen, Higgs-Teilchen, Neutrinos und Quarks – das Interesse an den kleinsten uns bekannten Teilchen ist seit Jahrzehnten ungebrochen und rückt damit auch die Laboratorien in den Blick, die an die Grenzen der Physik vorstoßen: Neben dem größten Experimentierfeld, das wir haben – dem Universum selbst - sind es die gigantischen Maschinen der Elementarteilchenphysik in Großforschungseinrichtungen wie dem CERN und dem DESY. Mit ihnen versuchen Forscher weltweit unter Einsatz extrem hoher Energien Zustände zu simulieren, wie sie zum Beginn unseres Universums kurz nach d...

  8. OHOLO Conference (36th): Multidisciplinary Approaches to Cholinesterase Functions Held in Eilat, Israel on April 6 - 10, 1992.

    Science.gov (United States)

    1992-04-10

    Caenorhabditis elegans. A4. - Bank, Gina - Cloning and Analysis of the Acetylcholinesterase Gene of Ceratitis capitata (medfly) A5.- Bartels, C., T...FOR THE 36th OHOLO CONFERENCE CLONING AND ANALYSIS OF THE ACETYLCHOLINESTERASE GENE OF Ceratitis capitata (inedfly) Gina Banks, I.M.B.B., P.O. B. 1527...Heraklion 711 10, Crete, Greece Sequence data has been determined for the acetylcholinesterase (AChE) gene from Ceratitis capitata (medfly), the

  9. Beratung auf Facebook und Twitter? Wie virtuelle Beratungsangebote auf die neuen Leitmedien reagieren können

    Directory of Open Access Journals (Sweden)

    Heinz Thiery

    2011-10-01

    Full Text Available Facebook und Twitter erleben aktuell einen enormen Zulauf. Beide konkurrieren um die Vorherrschaft als Leitmedium des WEB 2.0. Mit ihrem Angebotsmix sind sie nicht nur für die Mehrheit der Internetnutzer attraktiv, sie prägen darüber hinaus die Nutzungsgewohnheiten, die sich in Form einer spezifischen „Internetkultur“ etablieren. Zudem forcieren die Leitmedien technische Trends wie beispielsweise das „mobile computing“. Der Artikel beschäftigt sich mit der Frage, welche Auswirkungen diese Entwicklungen auf die professionellen Onlineberatungsangebote hat und wie ihnen begegnet werden kann.

  10. 78 FR 68021 - Notice of Affirmation of Addition of a Treatment Schedule for Methyl Bromide Fumigation of...

    Science.gov (United States)

    2013-11-13

    ... species, Ceratitis capitata (Mediterranean fruit fly) and Anastrepha fraterculus (South American fruit fly... bromide treatment schedule to mitigate risk from C. capitata and A. fraterculus, as described in the...

  11. 76 FR 80870 - Notice of Determination of Pest-Free Areas in Mendoza Province, Argentina

    Science.gov (United States)

    2011-12-27

    ... additional areas as pest-free areas for Mediterranean fruit fly (Ceratitis capitata) in Argentina. We... that, in addition to the pest-free status for C. capitata, the Mendoza province of Argentina also...

  12. Quantenfeldtheorie wie man beschreibt, was die Welt im Innersten zusammenhält

    CERN Document Server

    Fritzsch, Harald

    2015-01-01

    Die Geheimnisse des Mikrokosmos aufzudecken, hat die Physiker seit Jahrhunderten fasziniert. Doch obwohl in den vergangenen Jahrzehnten viele interessante Entdeckungen gemacht wurden, bleiben noch Fragen offen, die Harald Fritzsch in diesem Buch von der physikalischen und mathematischen Seite beleuchtet: - Haben die Physiker mit der Standardtheorie der Elementarteilchenphysik schon eine exakte Beschreibung der Teilchenwelt gefunden? - Gibt es wirklich eine „Große Vereinigung“ aller fundamentalen Kräfte? - Und sind die Leptonen, (wie das Elektron), oder die Bausteine der Hadronen, (die Quarks), vielleicht gar nicht wirklich punktförmig, sondern kleine eindimensionale Objekte, die man als „Strings“ bezeichnet? Dieses Buch setzt mathematische Vorbildung voraus und versteht sich als Überblick über die modernen Grundlagen der Quantenfeldtheorie und als Diskussionsbeitrag. Somit eignet sich das Werk insbesondere auch als Überblick und Wiederholung für Physiker. Harald Fritzsch ist bereits als Auto...

  13. Effect of the essential volatile oils isolated from Thymbra capitata (L. Cav. on olive and sunflower oils

    Directory of Open Access Journals (Sweden)

    Pedro, L.

    2003-09-01

    Full Text Available The chemical composition of the volatile constituents of the oils isolated from different parts of Thymbra capitata collected at different developmental stages were analysed by GC and GC/MS. The antioxidant ability of the oils isolated from T. capitata was evaluated determining the peroxide values, on olive and sunflower oils, stored at 60 ºC. These peroxide values were compared with those obtained when BHT, carvacrol and control (without adding antioxidants were used and subjected to the same conditions. The best yield oil was obtained from the whole aerial part of T. capitata collected during the flowering phase. The major component of the oils was carvacrol. Relative high amounts of p-cymene, γ-terpinene and β-caryophyllene were also found. BHT revealed to be the best antioxidant when the olive oil was used. On sunflower oil, the antioxidant ability of BHT was not so evident, being the carvacrol-rich essential oils of T. capitata or carvacrol more important antioxidants.Se analizaron, mediante GC y GC/MS, los componentes volátiles de aceites aislados de las distintas partes de la Thymbra capitata, recogida en diferentes etapas de desarrollo. Se evaluó la actividad antioxidante de estos aceites de la T. capitata, midiendo el índice de peróxidos, en aceites de oliva y girasol, almacenados a 60 ºC. Estos índices de peróxidos se compararon con los obtenidos cuando no se agregó ningún antioxidante (control y cuando se utilizó BHT o carvacrol, en las mismas condiciones de almacenamiento. El mayor rendimiento en aceite se obtuvo de la parte aérea de T. capitata recogida durante la etapa de floración. El componente mayoritario de los aceites fue el carvacrol. También se encontraron, cantidades relativamente elevadas, de p-cimeno, γ-terpineno y β-cariofileno. El mejor antioxidante para el aceite de oliva resultó ser el BHT. En el aceite del girasol, la actividad antioxidante del BHT no fue tan evidente, mientras que el

  14. Phytotoxic Effects of Cinnamic Acid on Cabbage (Brassica oleracea var. capitata

    Directory of Open Access Journals (Sweden)

    Singh, N. B.

    2013-04-01

    Full Text Available The present study deals with the effects of exogenous application of cinnamic acid (CA on growth and metabolism in growing seedlings of Brassica oleracea var. capitata (cabbage in hydroponic culture. CA was added at 0.5, 1.0 and 1.5 mM concentrations. CA has shown inhibitory effects on shoot and root length, fresh and dry weight of seedlings. CA significantly decreased the photosynthetic pigments, nitrate reductase activity and protein content. Graded concentrations of CA increased lipid peroxidation and sugar content. The increasing concentrations of CA significantly increased the antioxidative enzyme activities viz. superoxide dismutase, catalase, peroxidase against the oxidative stress caused by CA.

  15. Metallothionein induction and antioxidative responses in the estuarine polychaeta Capitella capitata (Capitellidae)

    Institute of Scientific and Technical Information of China (English)

    Suriya J; S Bharathiraja; V Sekar; R Rajasekaran

    2012-01-01

    Objective: To evaluate the antioxidant response induced by copper (Cu) exposure inCapitella capitata. The capacity of Cu bioaccumulation was also evaluated through the metal quantification. Methods: Worms were exposed to different concentrations of Cu 50, 100, and 200 μg/L for 7, 14 and 21 days respectively. In all the assays, control groups were run in parallel, employing only saline water (10%). The concentrations of Cu in the digested acidic solutions of worms were determined by using the inductively coupled plasma-Optical emission spectrophotometry. The total protein content and other anti oxidant enzyme activities such as superoxide dismutase (SOD), catalase (CAT) and glutathione S-transferase (GST) were recorded for the experiment period. Results: Bioaccumulation of Cu metals in polychaetes was increased parallel to exposure time. A peak concentration (197.30±12.32 μg/g) of metallothioneins (MTs) was registered at 14th day of 100 μg/L group compared to control group (37.29 μg/g). Catalase activities (CAT) in Cu treated-worms were increased significantly (P<0.05) after 7 and 14 days of exposure. The concentration of Cu had significant (P<0.05) influences on the activity of SOD. The same physiological activity was recorded in GST evaluation. Conclusions: These results of biochemical variables in C. capitata suggest us a useful model species for monitoring of environmental disturbance by heavy metal pollution. Antioxidant defenses were confirmed as sensitive biomarkers for metal stress in polychaete worms.

  16. Bleached Porites compressa and Montipora capitata corals catabolize δ13C-enriched lipids

    Science.gov (United States)

    Grottoli, Andréa G.; Rodrigues, Lisa J.

    2011-09-01

    Corals rely on stored energy reserves (i.e., lipids, carbohydrates, and protein) to survive bleaching events. To better understand the physiological implications of coral bleaching on lipid catabolism and/or synthesis, we measured the δ13C of coral total lipids (δ13CTL) in experimentally bleached (treatment) and non-bleached (control) Porites compressa and Montipora capitata corals immediately after bleaching and after 1.5 and 4 months of recovery on the reef. Overall δ13CTL values in treatment corals were significantly lower than in control corals because of a 1.9 and 3.4‰ decrease in δ13CTL immediately after bleaching in P. compressa and M. capitata, respectively. The decrease in δ13CTL coincided with decreases in total lipid concentration, indicating that corals catabolized δ13C-enriched lipids. Since storage lipids are primarily depleted during bleaching, we hypothesize that they are isotopically enriched relative to other lipid classes. This work further helps clarify our understanding of changes to coral metabolism and biogeochemistry when bleached and helps elucidate how lipid classes may influence recovery from bleaching and ultimately coral survival.

  17. Jurisprudence. Repositioning of cables and pipes. Who decides and who pays?; Jurisprudentie. Kabels en leidingen verleggen. Wie bepaalt en betaalt?

    Energy Technology Data Exchange (ETDEWEB)

    Roggenkamp, M. [Brinkhof Advocaten, Amsterdam (Netherlands)

    2011-09-15

    Underground cables and pipes sometimes need to be repositioned to make room for buildings. Can any exploiter of pipes be obliged to do this. And if this is the case, who should pay for it? Every year these questions are causing new disputes. [Dutch] Ondergrondse kabels en leidingen moeten soms worden verlegd om plaats te maken voor bebouwing. Kan een leidingexploitant hiertoe verplicht worden? En zo ja, wie moet dat betalen? Hierover ontstaan jaarlijks geschillen.

  18. Rekodieren im Deutschen und Englischen: Wie rekodieren Englischlerner/-innen mit deutscher Muttersprache englische Wörter?

    OpenAIRE

    Treutlein, Anke

    2011-01-01

    In den klassischen Lesemodellen wie dem Zwei-Wege-Modell oder dem konnektionistischen Modell wird davon ausgegangen, dass das Rekodieren in allen Orthographien vergleichbar erfolgt. Empirische Befunde sprechen jedoch dafür, dass das Rekodieren von Merkmalen der jeweiligen Orthographie beeinflusst ist. Insbesondere die Konsistenz einer Orthographie scheint bedeutsam für Rekodierprozesse zu sein. Nur wenige Theorien und Modelle berücksichtigen allerdings diese Interdependenz: die Theorie der or...

  19. Field trials of solid triple lure (trimedlure, methyl eugenol, raspberry ketone, and DDVP) dispensers for detection and male annihilation of Ceratitis capitata (Wiedemann), Bactrocera dorsalis (Hendel) and Bactrocera cucurbit

    Science.gov (United States)

    Solid Mallet TMR (trimedlure [TML], methyl eugenol [ME], raspberry ketone [RK]) wafers and Mallet CMR (ceralure, ME, RK, benzyl acetate) wafers impregnated with DDVP insecticide were evaluated in traps as potential detection and male annihilation devices. Comparisons were made with 1) liquid lure a...

  20. Plastid transformation in cabbage (Brassica oleracea L. var. capitata L.) by the biolistic process.

    Science.gov (United States)

    Tseng, Menq-Jiau; Yang, Ming-Te; Chu, Wan-Ru; Liu, Cheng-Wei

    2014-01-01

    Cabbage (Brassica oleracea L. var. capitata L.) is one of the most important vegetable crops grown worldwide. Scientists are using biotechnology in addition to traditional breeding methods to develop new cabbage varieties with desirable traits. Recent biotechnological advances in chloroplast transformation technology have opened new avenues for crop improvement. In 2007, we developed a stable plastid transformation system for cabbage and reported the successful transformation of the cry1Ab gene into the cabbage chloroplast genome. This chapter describes the methods for cabbage transformation using biolistic procedures. The following sections are included in this protocol: preparation of donor materials, coating gold particles with DNA, biolistic bombardment, as well as the regeneration and selection of transplastomic cabbage plants. The establishment of a plastid transformation system for cabbage offers new possibilities for introducing new agronomic and horticultural traits into Brassica crops.

  1. Origanum vulgare and Thymbra capitata Essential Oils from Spain: Determination of Aromatic Profile and Bioactivities.

    Science.gov (United States)

    Carrasco, Alejandro; Perez, Enrique; Cutillas, Ana-Belen; Martinez-Gutierrez, Ramiro; Tomas, Virginia; Tudela, Jose

    2016-01-01

    Oregano (Thymbra capitata and Origanum vulgare) essential oils (EOs), cultivated and extracted in the South-East of Spain, were analysed by GC/MS to determine their composition. (E)-β-Caryophyllene (0.5-4.9%), thymol (0.2-5.8%), p-cymene (3.8-8.2%), γ-terpinene (2.1-10.7%) and carvacrol (58.7-77.4%) were determined as the main molecules. This characterisation was completed with enantioselective gas chromatography, where (-)-(E)-β-caryophyllene, (+)-a- pinene and (+)-β-pinene were determined as the main enantiomers. Antioxidant activity was evaluated positively by several methods, accounting for activity against free radicals and reducing power. Important inhibitory activity on lipoxygenase (LOX) and acetylcholinesterase (AChE) was observed supporting potential anti-inflammatory, anti-Alzheimer and insecticidal activities, mainly due to carvacrol. These properties support the potential use of oregano EOs as natural cosmetic and natural pharmaceutical ingredients.

  2. Experimental and DFT studies on the antioxidant activity of a C-glycoside from Rhynchosia capitata

    Science.gov (United States)

    Praveena, R.; Sadasivam, K.; Kumaresan, R.; Deepha, V.; Sivakumar, Raman

    2013-02-01

    Rhynchosia capitata (=Glycine capitata) Heyne ex roth, was found to possess polyphenolics including flavonoids, which acts as potential antioxidant. The study of ethanolic extract of roots and leaves reveals that the leaves possess high polyphenolics including flavonoids than roots. This was also confirmed by DPPH radical scavenging activity. Leaf powder of the plant was extracted with different solvents by soxhlet apparatus in the order of increasing polarity. The DPPH scavenging activity of methanol fraction was found to be high compared to the crude extract and other fractions. Nitric oxide scavenging activity was dominant in chloroform fraction compared to methanol fraction. Presence of flavonoids especially vitexin, a C-glycoside in methanol and chloroform fractions were confirmed by high pressure thin layer chromatography (HPTLC) analysis. The structural and molecular characteristics of naturally occurring flavonoid, vitexin was investigated in gas phase using density functional theory (DFT) approach with B3LYP/6-311G(d,p) level of theory. Analysis of bond dissociation enthalpy (BDE) reveals that the OH site that requires minimum energy for dissociation is 4'-OH from B-ring. To explore the radical scavenging activity of vitexin, the adiabatic ionization potential, electron affinity, hardness, softness, electronegativity and electrophilic index properties were computed and interpreted. The nonvalidity of Koopman's theorem has been verified by the computation of Eo and Ev energy magnitudes. Interestingly, from BDE calculations it was observed that BDE for 4'-OH, 5-OH and 7-OH are comparatively low for vitexin than its aglycone apigenin and this may be due to the presence of C-8 glucoside in vitexin. To substantiate this, plot of frontier molecular orbital and spin density distribution analysis for neutral and the corresponding radical species for the compound vitexin have been presented.

  3. Germination ecology of Rhynchosia capitata: an emerging summer weed in Asia Ecologia da germinação de Rhynchosia capitata: planta daninha emergente durante o verão na Ásia

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    H.H. Ali

    2013-06-01

    Full Text Available Rhynchosia capitata is becoming an increasing problem in summer crops, such as cotton, soybean, pearl millet and mungbean in many Asian countries. Laboratory and greenhouse studies have been conducted to determine the effects of several environmental factors on seed germination patterns and seedling emergence of R. capitata. We investigated whether the diverse ecological factors such as temperature, light, salinity, moisture stress, pH, and soil depth affected germination and seedling emergence of R. capitata. Germination increased as temperature increased from 25ºC and significantly reduced at 45ºC. Presence or absence of light did not influence germination. Germination of R. capitata was sensitive to increased salt and moisture stress, as well as to seed burial depth. Only 48% of seeds germinated at 150 mM salt concentration compared to 100% in control (distilled water. Similarly, 15% of seeds germinated at an osmotic potential of ‑0.8 MPa compared to 88% at ‑0.2 MPa. The optimum pH for seed germination of R. capitata was 7 (98% germination, but the seeds also germinated at lower level of pH 5 (85% and at higher level of pH 10 (75%. In seed burial trial, maximum seedling emergence of 93% occurred at 2 cm depth, and seedling did not emerge from a depth of 12 cm. The high germination ability of R. capitata under a wide range of ecological factors suggests that this species is likely to be the one to cause more problems in a near future, if not managed appropriately.A Rhynchosia capitata vem se tornando um problema crescente para culturas de verão como algodão, soja, milheto e feijão-mungo em muitos países asiáticos. Foram feitos estudos de laboratório e estufa para determinar os efeitos de vários fatores ambientais sobre os padrões de germinação das sementes e emergência das plântulas de R. capitata. Diversos fatores ecológicos, tais como, temperatura, luz, salinidade, estresse hídrico, pH e profundidade do solo foram

  4. Discovery and Understanding of the Ambient-Condition Degradation of Doped Barium Cerate Proton-Conducting Perovskite Oxide in Solid Oxide Fuel Cells

    NARCIS (Netherlands)

    Yan, N.; Zeng, Y.; Shalchi, B; Wang, W.; Gao, T; Rothenberg, G.; Luo, J.L.

    2015-01-01

    Proton-conducting perovskite oxides such as doped barium cerate and barium zirconate are promising electrolytes for solid oxide fuel cells (SOFCs). Here we report that the typical high performance proton conductor, BaZr0.1Ce0.7Y0.2O3±δ (BZCY), is prone to physical, chemical and thereby electrochemic

  5. Dum lucet - bibamus sodales. Ob und wie wir Archäologen die Alten verstehen

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    Iva Mikl Curk

    2004-12-01

    Full Text Available In welchem Umfang können die Leute des Altertums von Achäologen verstanden werden? Wir wählen zum Titel des Aufsatzes einen Teil der Inschrift vom ACO-Becher aus Virunum (jetzt im Landesmuseum Klagenfurt, da wir ein Thema über die materielle Kultur aus der Römerzeit bearbeiten. Die Römerzeit ist nämlich die älteste Periode in der Geschichte des östlichen Alpen- und nordadriatischen Raums, so auch Sloweniens, und zeugt auch durch direkte schriftliche Quellen von sich selbst. So kann das Verfolgen der Tatsachen, wie gewisse Erscheinungsformen an Artefakten durch die archäologische Forschung verstanden sind, besonders aufregend werden. Wir richten unsere Aufmerksamkeit auf drei Beispiele, die in vergangenen Jahrzehnten durch Publikation etwas mehr ins Blickfeld geraten sind. Auch Trinkgefäße befinden sich darunter. An erster Stelle ist die Rede vom glirarium - dem töpfernen »Käfig« für Siebenschläfer - von Poetovio (heute Ptuj, Slowenien. Das Gefäß wurde als Graburne im (späten 2. Jh. verwendet. Zu dieser Art von Verwendung wurde in den bisherigen Bearbeitungen nur einmal Stellung genommen (Mikl Curk 1986 und die Vermutung geäußert, man habe zur Zeit der Grablegung möglicherweise den wahren Zweck des Gefäßes nicht mehr gekannt. Doch heute wird eine neue Deutung vorgeschlagen: Beim Vorkommen eines solchen Gefäßes im Grab soll es sich um ein Symbol handeln. Dieses Symbol soll, mit gewissen weiteren aus den römischen Brandgräbern in Slowenien essbaren Grabbeigaben (Muscheln, Schnecken, den Gedanken an das - unter der Erscheinung des Todes - schlummernde Leben darstellen. Die verschlossenen Muschel- und Schneckenschalen haben ja die Form eines leblosen Steines, so wie auch der Siebenschläfer während des Winterschlafes tot zu sein scheint. Solche Beigaben brachten uns auf den Gedanken, eine weitere Zahl von Geschirr-Beigaben auch in solch einem Sinn zu deuten (Mikl Curk 1985; 1985a; 1999; 2001. Diese eventuell sogar in

  6. Respuesta de ecotipos de Stylosanthes capitata a dosis de cobre en solución nutritiva

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    Pizarro Esteban A.

    1986-09-01

    Full Text Available La solución nutritiva de Arnon y Hoagland se modificó a 1/10 para simular las condiciones de acidez y fertilidad del suelo de las sabanas bien drenadas isohipertérmicas de los Llanos Orientales de Colombia. Se usaron seis concentraciones de cobre (0,0.05, 0.1, 0.2, 0.4 Y 0.8 ppm. la solución nutritiva se renovó cada 15 días y se aplicaron nitrógeno (14 ppm/semana e hierro (1 ppm, 3/semana. La cantidad de materia seca producida por los ecotipos y el cultivar Capica en los tratamientos de 0.2, 0.4 Y0.8 ppm Cu, fue relativamente te nula. La producción de materia seca de la parte aérea, raíz y total de la planta fue diferente en los ecotipos de S capitata. Así mismo hubo diferencias significativas en la concentración, absorción y translocación del cobre por lo ecotipos y el cultivar Capica. El requerimiento diferencial de cobre, manifestado en la producción de materia seca en la parte aérea en el nivel 0.05 ppr por los ecotipos CIAT 1693 y 1728 por el cultivar Capica (CIAT 102801, se define como el resultado de las relaciones evolutivas de cada ecotipo con el nivel de cobre disponible en la solución del suelo.In the greenhouse ecotypes of Stylosanthes capitata were cultivated to observe their behavior under different copper concentrations. A nutrient solution based on Arnon and Hoagland's (1/10 was used to simulate the acidity and the fertility of well drained savannas of the Eastern Planes of Colombia. Six concentrations of copper were used (O, 0.05, 0.1, 0.2, 0.4 and 0.8 ppm with rewal of the nutrient solution every 15 days, and applications of nitrogen (14 ppm/week and iron (1 ppm, three times/week. The dry matter quantity produced by the ecotypes and the Capica cultivar under treatments of 0.2, 0.4 and 0.8 ppm Cu, relatively nill. A differential behavior of Stylosanthes capitata ecotypes responses to applied copper was observed in relation to dry matter production in aerial, part, root and total plant. So, there were significant

  7. Physikalische Therapie bei Nacken-Schulterschmerzen: wofür, wobei, wie häufig verordnen

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    Heiller I

    2007-01-01

    Full Text Available Fragestellung: In der täglichen klinischen Routine führen der Einsatz von Elektrotherapie, Ultraschall und anderen Wärmeanwendungen wie auch die Massage zu einer Verbesserung unterschiedlicher Funktionsparameter des Bewegungsapparates, des Schmerzes und der Befindlichkeit. Dies gilt für das gesamte muskuloskelettale System, insbesondere auch für den unspezifischen Nacken-Schulterschmerz. Die einzelnen therapeutischen Maßnahmen werden im Detail beschrieben. Das Ziel der Physikalischen Medizin ist es, die Dysfunktion zu identifizieren und durch eine gezielte Behandlung zu verbessern. In weiterer Folge soll die Angst vor Bewegung durch aktivierende Trainingsmaßnahmen genommen werden. Aufgrund der individuellen Problematik jedes einzelnen Patienten gibt es kein allgemeingültiges Behandlungskonzept, dieses muß gemäß den untersuchten Dysfunktionen an den Patienten angepaßt werden. Die Wirksamkeit der eingesetzten Therapien beruht teilweise auf Empirie; gezielte Evaluationsmaßnahmen, die die therapeutische Effektivität beweisen, sind rar. Insbesondere multimodale Interventionen wie etwa die Kombination von Thermotherapien mit Massagen oder auch die Kombination mit Übungsbehandlungen im Sinne der Kräftigung und Dehnung sowie die Häufigkeit ihrer Anwendung sind bis dato in der Literatur wenig erforscht und werden diskutiert.

  8. 78 FR 23208 - Importation of Fresh Oranges and Tangerines From Egypt Into the United States

    Science.gov (United States)

    2013-04-18

    ... fly (Ceratitis capitata); however, imports of oranges from Egypt were suspended in July 2002 due to... with 7 CFR part 305 for C. capitata and B. zonata; and The oranges and tangerines must be accompanied... has undergone treatment for C. capitata and B. zonata in accordance with 7 CFR part 305, with...

  9. 77 FR 22465 - Importation of Fresh Pitaya Fruit From Central America Into the Continental United States

    Science.gov (United States)

    2012-04-16

    ..., Ceratitis capitata, Dysmicoccus neobrevipes, and Planococcus minor. (a) Monitoring and oversight. (1) The... crop itself, must not shade the crop during the day. No C. capitata or A. ludens host plants may be.... capitata and A. ludens--(1) Pest- free places of production. (i) Beginning at least 1 year before...

  10. A catalog of bird specimens associated with Prince Maximilian of Wied-Neuwied and potential type material in the natural history collection in Wiesbaden

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    Dorothee Hoffmann

    2013-11-01

    Full Text Available Bird specimens collected by 19th century explorer and ornithologist Prince Maximilian of Wied-Neuwied form one of the foundation collections of the American Museum of Natural History in New York. However, parts of his collection remained in Germany and came to the Museum Wiesbaden. Since Wied described numerous new species without designating types, some of these specimens might be type material. Here we present a catalog of the 30 Wiesbaden specimens associated with him and discuss their potential type status. We conclude that 17 individuals in 11 species are potential type specimens that should be considered in future taxonomic work.

  11. A catalog of bird specimens associated with Prince Maximilian of Wied-Neuwied and potential type material in the natural history collection in Wiesbaden.

    Science.gov (United States)

    Hoffmann, Dorothee; Geller-Grimm, Fritz

    2013-01-01

    Bird specimens collected by 19(th) century explorer and ornithologist Prince Maximilian of Wied-Neuwied form one of the foundation collections of the American Museum of Natural History in New York. However, parts of his collection remained in Germany and came to the Museum Wiesbaden. Since Wied described numerous new species without designating types, some of these specimens might be type material. Here we present a catalog of the 30 Wiesbaden specimens associated with him and discuss their potential type status. We conclude that 17 individuals in 11 species are potential type specimens that should be considered in future taxonomic work.

  12. Impact of copper toxicity on stone-head cabbage (Brassica oleracea var. capitata) in hydroponics.

    Science.gov (United States)

    Ali, Sajid; Shahbaz, Muhammad; Shahzad, Ahmad Naeem; Khan, Hafiz Azhar Ali; Anees, Moazzam; Haider, Muhammad Saleem; Fatima, Ammara

    2015-01-01

    Arable soils are frequently subjected to contamination with copper as the consequence of imbalanced fertilization with manure and organic fertilizers and/or extensive use of copper-containing fungicides. In the present study, the exposure of stone-head cabbage (Brassica oleracea var. capitata) to elevated Cu(2+) levels resulted in leaf chlorosis and lesser biomass yield at ≥2 µ M. Root nitrate content was not statistically affected by Cu(2+) levels, although it was substantially decreased at ≥5 µ M Cu(2+) in the shoot. The decrease in nitrate contents can be related to lower nitrate uptake rates because of growth inhibition by Cu-toxicity. Shoot sulfate content increased strongly at ≥2 µ M Cu(2+) indicating an increase in demand for sulfur under Cu stress. Furthermore, at ≥2 µM concentration, concentration of water-soluble non-protein thiol increased markedly in the roots and to a smaller level in the shoot. When exposed to elevated concentrations of Cu(2+) the improved sulfate and water-soluble non-protein thiols need further studies for the evaluation of their direct relation with the synthesis of metal-chelating compounds (i.e., phytochelatins).

  13. Impact of copper toxicity on stone-head cabbage (Brassica oleracea var. capitata in hydroponics

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    Sajid Ali

    2015-08-01

    Full Text Available Arable soils are frequently subjected to contamination with copper as the consequence of imbalanced fertilization with manure and organic fertilizers and/or extensive use of copper-containing fungicides. In the present study, the exposure of stone-head cabbage (Brassica oleracea var. capitata to elevated Cu2+ levels resulted in leaf chlorosis and lesser biomass yield at ≥2 µ M. Root nitrate content was not statistically affected by Cu2+ levels, although it was substantially decreased at ≥5 µ M Cu2+ in the shoot. The decrease in nitrate contents can be related to lower nitrate uptake rates because of growth inhibition by Cu-toxicity. Shoot sulfate content increased strongly at ≥2 µ M Cu2+ indicating an increase in demand for sulfur under Cu stress. Furthermore, at ≥2 µM concentration, concentration of water-soluble non-protein thiol increased markedly in the roots and to a smaller level in the shoot. When exposed to elevated concentrations of Cu2+ the improved sulfate and water-soluble non-protein thiols need further studies for the evaluation of their direct relation with the synthesis of metal-chelating compounds (i.e., phytochelatins.

  14. The nitrogen and nitrate economy of butterhead lettuce (Lactuca sativa var capitata L).

    Science.gov (United States)

    Broadley, Martin R; Seginer, Ido; Burns, Amanda; Escobar-Gutiérrez, Abraham J; Burns, Ian G; White, Philip J

    2003-09-01

    Quantifying and simulating the relationships between crop growth, total-nitrogen (total-N) and nitrate-N (NO3--N) concentration can improve crop nutritional husbandry. In this study, the relationship between shoot relative growth rate (RGR) and shoot total-N, organic-N and NO3--N concentration of hydroponically-grown lettuce (Lactuca sativa var. capitata L. cv. Kennedy) was described and simulated. Plants were grown hydroponically for up to 74 d. Nitrogen was supplied throughout (control; T1), or removed at 35 d (T2) and 54 d (T3), respectively, after sowing. The organic-N and NO3--N concentration declined in the shoots of control plants with growth, until commercial maturity approached when organic-N and NO3--N concentration increased. There were sub-linear relationships between both total-N and organic-N concentration, and shoot RGR, in the N-limited treatments, i.e. shoot RGR approached an asymptote at high shoot N concentration. The proportional effects of total-N and organic-N concentration on shoot RGR were independent of plant age. A dynamic simulation model ('Nicolet'), derived previously under different conditions, was used to simulate the growth, dry matter content, organic-N, and NO3--N concentration of lettuce grown under the extreme N-stress conditions experienced by the plants. In view of the largely successful fitting of the model to experimental data, the model was used to interpret the results. Suggestions for model improvement are made.

  15. Formation and structural organization of the egg-sperm bundle of the scleractinian coral Montipora capitata

    Science.gov (United States)

    Padilla-Gamiño, J. L.; Weatherby, T. M.; Waller, R. G.; Gates, R. D.

    2011-06-01

    The majority of scleractinian corals are hermaphrodites that broadcast spawn their gametes separately or packaged as egg-sperm bundles during spawning events that are timed to the lunar cycle. The egg-sperm bundle is an efficient way of transporting gametes to the ocean surface where fertilization takes place, while minimizing sperm dilution and maximizing the opportunity for gamete encounters during a spawning event. To date, there are few studies that focus on the formation and structure of egg-sperm bundle. This study explores formation, ultrastructure, and longevity of the egg-sperm bundle in Montipora capitata, a major reef building coral in Hawai`i. Our results show that the egg-sperm bundle is formed by a mucus layer secreted by the oocytes. The sperm package is located at the center of each bundle, possibly reflecting the development of male and female gametes in different mesenteries. Once the egg-sperm bundle has reached the ocean surface, it breaks open within 10-35 min, depending on the environmental conditions (i.e., wind, water turbulence). Although the bundle has an ephemeral life span, the formation of an egg-sperm bundle is a fundamental part of the reproductive process that could be strongly influenced by climate change and deterioration of water quality (due to anthropogenic effects) and thus requires further investigation.

  16. Moscas-das-frutas (Diptera: Tephritidae em um pomar de goiabeira, no semiárido brasileiro

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    Elton Lucio Araujo

    2013-06-01

    Full Text Available As moscas-das-frutas (Diptera: Tephritidae são pragas-chave na cultura da goiabeira Psidium guajava L., com predominância de diferentes espécies de acordo com a região produtora no Brasil. Os objetivos do presente trabalho foram conhecer a diversidade e analisar parâmetros faunísticos das moscas-das-frutas obtidas em um pomar de goiabeira, no município de Cruzeta, Rio Grande do Norte, situado no semiárido brasileiro. As moscas-das-frutas foram coletadas semanalmente, com auxílio de armadilhas McPhail, tendo como atrativo proteína hidrolisada a 5% v/v. Foram registradas cinco espécies no pomar estudado: Ceratitis capitata (Wied., Anastrepha zenildae Zucchi, Anastrepha sororcula Zucchi, Anastrepha obliqua (Macquart e Anastrepha dissimilis Stone. Ceratitis capitata foi a espécie mais frequente, constante e dominante, considerada como uma praga invasiva, potencial em pomares de goiabeira no semiárido brasileiro.

  17. Irradiation of mangoes as a quarantine treatment

    Energy Technology Data Exchange (ETDEWEB)

    Bustos R, M.E.; Enkerlin H, W.; Toledo A, J.; Reyes F, J.; Casimiro G, A

    1991-06-15

    This research project was conducted following guidelines of research protocols for post-harvest treatments developed by the United States Department of Agriculture CUSA. Laboratory bioassays included the irradiation of mangoes infested with third instar larvae of Anastrepha serpentina (Wied), A. ludens (Loew), A. obliqua (Macquart) and Ceratitis capitata (Wied) , at doses from 10 to 250 Gy. Irradiation doses were applied using a Co-60 AECL Model JS-7400 irradiator. The design was chosen to obtain a maximum to minimum ratio equal to, or less than, 1.025. C. capitata was the species most tolerant to irradiation. A dose of 60 Gy applied to third instar fruit fly larvae sterilized this species and prevented emergence of adults of the other three species. A dose of 250 Gy was required to prevent emergence of C. capitata. In fertility tests using emerged adults of A . Iudens, and A. obliqua a dose of 30 Gy gave 45 % and 27 % fertility, respectively. Adults of A. serpentina that emerged, died before reaching sexual maturity. The confirmatory tests, at probit-9 security level, were done at 100 Gy for the three species of Anastrepha and at 150 Gy for C. capitata. The quality of mangoes irradiated up to 1000 Gy was evaluated by chemical, physiological, and sensorial tests. The determination of vitamin C indicated that there was no loss of the nutritive value of the fruit. It also was observed that fruit metabolism was not accelerated since no significant increase in respiration or transpiration was registered and consumers accepted both treated and untreated fruit in the same way. (Author)

  18. Nitrate reductase activity in cabbage (Brassica oleracae var. capitata seedlings affected by the different nitrogen fertilizer forms

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    Metin Turan

    2013-12-01

    Full Text Available The effect of different nitrogen fertilizer (potassium nitrate, ammonium nitrate, ammonium sulphate, urea and farmyard manure on nitrate reductase activity in cabbage (Brassica oleracea var. capitata seedlings were studied. pH of the plant growth niedia was higher in the nitrate fertilizer treatment than the ammonium and other fertilizer forms. NO3--N application increased NRA in plant, but NH4+-N decreased NRA in plant. Harvesting date and different fertilizer doses increased NRA while NH4+-N decreased plant nitrate uptake. There was a significant relationship between NRA and fertilizer types.

  19. Tissue loss (white syndrome) in the coral Montipora capitata is a dynamic disease with multiple host responses and potential causes

    Science.gov (United States)

    Work, Thierry M.; Russell, Robin; Aeby, Greta S.

    2012-01-01

    Tissue loss diseases or white syndromes (WS) are some of the most important coral diseases because they result in significant colony mortality and morbidity, threatening dominant Acroporidae in the Caribbean and Pacific. The causes of WS remain elusive in part because few have examined affected corals at the cellular level. We studied the cellular changes associated with WS over time in a dominant Hawaiian coral, Montipora capitata, and showed that: (i) WS has rapidly progressing (acute) phases mainly associated with ciliates or slowly progressing (chronic) phases mainly associated with helminths or chimeric parasites; (ii) these phases interchanged and waxed and waned; (iii) WS could be a systemic disease associated with chimeric parasitism or a localized disease associated with helminths or ciliates; (iv) corals responded to ciliates mainly with necrosis and to helminths or chimeric parasites with wound repair; (v) mixed infections were uncommon; and (vi) other than cyanobacteria, prokaryotes associated with cell death were not seen. Recognizing potential agents associated with disease at the cellular level and the host response to those agents offers a logical deductive rationale to further explore the role of such agents in the pathogenesis of WS in M. capitata and helps explain manifestation of gross lesions. This approach has broad applicability to the study of the pathogenesis of coral diseases in the field and under experimental settings.

  20. Highly differentiated populations of the freshwater diatom Sellaphora capitata suggest limited dispersal and opportunities for allopatric speciation.

    Science.gov (United States)

    Evans, Katharine M; Chepurnov, Victor A; Sluiman, Hans J; Thomas, Sindu J; Spears, Bryan M; Mann, David G

    2009-08-01

    The diversities and distributions of diatoms are much more complex than was ever imagined. To understand the underlying mechanisms, research must focus on evolutionary processes occurring at a population level and employ sufficiently informative molecular markers. Using ten microsatellites and ITS rDNA sequence data, we investigated the genetic structure of populations of the benthic freshwater diatom Sellaphora capitata (until 2004 a cryptic entity within the S. pupula agg. species complex). This is the first time that microsatellites have been used to investigate the genetic structure of any freshwater or benthic microalga. Using an integrated approach (morphology, DNA barcoding and specificity of the microsatellite primers), we verified the identity of 70 S. capitata isolates obtained from lakes in the UK, Belgium and Australia. Standardized F'(ST) values were very high (>0.4) and in Bayesian analyses, isolates clustered according to their country of origin, with limited evidence of admixture. However, selected isolates from all countries were sexually compatible, a result consistent with limited ITS divergence. Considering the apparent absence of desiccation-resistant resting stages in most diatoms, we conclude that such levels of differentiation are likely to be a consequence of limited dispersal. With restricted dispersal, previously unacknowledged opportunities for allopatric speciation exist, which may help to explain the huge extant diversity of diatoms.

  1. Description of a new ascaridoid parasite of Bothrops jararaca Wied (Reptilia, Ophidia in Brazil Descrição de um novo ascarídeo parasito de Bothrops jararaca Wied (Reptilia, Ophidia no Brasil

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    Liege R. Siqueira

    2005-09-01

    Full Text Available A new species of Ophidascaris Baylis, 1920 is described on the basis of specimens recovered from the pit viper Bothrops jararaca Wied, 1824 in Brazil. The new species referred here was compared to other of the genus that are the closest. The differentiation was based on reliable morphological characters, mainly on what refers to the spicular length, number and distribution of caudal papillae, location of the vulvar opening, eggs size and aspect of the labial indentations. Moreover, female specimens are the only to possess a most prominent and characteristic post-anal tubercle-like structure and when analyzed together with the males, that show a great number of pre-cloacal papillae, the presence of an unpaired papilla and also of a less conspicuous post-cloacal tubercle-like structure, permit the prompt identification of the new species.Uma nova espécie de Ophidascaris Baylis, 1920 é descrita, com base em exemplares parasitos de jararaca, Bothrops jararaca Wied, 1824 no Brasil. A nova espécie agora descrita foi comparada a outras do gênero que lhe são as mais próximas. A diferenciação foi baseada em caracteres morfológicos consistentes, principalmente nos que dizem respeito ao tamanho dos espículos, número e distribuição de papilas caudais, localização da abertura vulvar, tamanho dos ovos e aspecto das indentações labiais. Além disso, os exemplares fêmeas são os únicos no gênero a apresentarem uma estrutura em forma de tubérculo pós-anal bastante característica e proeminente, quando analisados junto aos exemplares machos, nos quais existe um grande número de papilas pré-cloacais, presença de papila caudal ímpar e, também, da estrutura pós-cloacal em forma de tubérculo, embora menos evidente que nas fêmeas, permitem a pronta identificação da espécie nova.

  2. Was bedeutet Adoleszenz, und wie kann man sie erforschen? What does Adolescence Mean, and How to Best Study It?

    Directory of Open Access Journals (Sweden)

    Susanne Unger

    2003-07-01

    Full Text Available Vera Kings Buch beschäftigt sich mit der Frage, wie die Adoleszenz in westlichen modernisierten Gesellschaften am besten theoretisch zu verstehen und zu untersuchen sei. In ihrer Analyse der Bedeutung und des Verlaufs der Adoleszenz berücksichtigt King stets die unterschiedlichen Bedingungen und Faktoren, welche auf die Adoleszenz einwirken, so u.a. die Generationszugehörigkeit, Geschlecht und sozialer Hintergrund bzw. soziale Ungleichheit. Während sich der erste Teil des Buches mit wissenschaftsgeschichtlichen und methodologischen Fragen zur Adoleszenzforschung befasst, stellt King im zweiten Teil konkrete Analysen zu drei Schwerpunktthemen vor: Familie, Körperbedeutungen und Peer Group in der Adoleszenz.Vera King’s book investigates how adolescence in Western modernised societies can best be conceived of and investigated. In her analysis of the meaning of adolescence and the processes which constitute it, King consistently takes into consideration the varying conditions and factors which influence adolescence such as, cohort, gender, and social inequality. Part I of the book deals with the history of science and methodological questions in the research on adolescence. In Part II, King analyses the role of family, of the body, and of peer groups during adolescence.

  3. Wie können Corporate Citizens voneinander lernen? : Ordonomische Anregungen für inter-organisationales Lernen im Global Compact der Vereinten Nationen

    OpenAIRE

    Hielscher, Stefan; Pies, Ingo; Beckmann, Markus

    2009-01-01

    "Der Global Compact der Vereinten Nationen versteht sich nicht als Regulierungsinitiative, sondern als freiwilliges Lern- und Dialogforum für Corporate Citizenship. Dieser Beitrag entwickelt Vorschläge, wie der Global Compact diesem Anspruch (noch besser) gerecht werden kann. Hier geht es um Strukturen in zweifacher Hinsicht: (a) Zum einen können Corporate Citizens besonders gut dann voneinander lernen, wenn sie sich über die strukturellen Bedingungen erfolgreicher - aber auch: fehlgeschlagen...

  4. Forschungsdatenmanagement als überregionale Aufgabe der Informationsversorgung - was kann eine Zentrale Fachbibliothek wie ZB MED Leibniz-Informationszentrum Lebenswissenschaften leisten?

    OpenAIRE

    Birte Lindstädt

    2015-01-01

    Forschungsdaten sind in aller Munde. Soll sich eine überregionale tätige Bibliothek deshalb auf diesem Feld engagieren? Und falls ja, für wen und wie? Auf der Grundlage einer breit angelegten Markt- und Zielgruppenanalyse strebt ZB MED ein zielgruppengerechtes Angebot im Bereich Forschungsdatenmanagement an, das sich in erster Linie an den Bedürfnissen der lebenswissenschaftlichen Fächer ausrichtet. Research Data Management is much talked about at present. Should a national library get ...

  5. Diet and feeding behavior of the White-naped Jay, Cyanocorax cyanopogon (Wied, 1821) (Aves, Passeriformes, Corvidae) in a disturbed environment in central Brazil

    OpenAIRE

    RAM Barros; CA Costa; MC Pascotto

    2014-01-01

    The White-naped Jay Cyanocorax cyanopogon (Wied, 1821) is an omnivorous and opportunistic species, with a multifarious diet. In view of the scarcity of available data in the literature, the aim was to investigate and describe the bird's diet, location of food items, foraging tactics, actual feeding behavior and intraspecific interactions, as a means of defining the items consumed. The study was carried out in a Cerrado area in the Araguaia Campus of the Federal University of Mato Grosso ̵...

  6. Antioxidant and Antiproliferative Activities of the Essential Oils from Thymbra capitata and Thymus Species Grown in Portugal

    Directory of Open Access Journals (Sweden)

    Maria Graça Miguel

    2015-01-01

    Full Text Available The antioxidant and antiproliferative activities of the essential oils from Thymbra capitata and Thymus species grown in Portugal were evaluated. Thymbra and Thymus essential oils were grouped into two clusters: Cluster I in which carvacrol, thymol, p-cymene, α-terpineol, and γ-terpinene dominated and Cluster II in which thymol and carvacrol were absent and the main constituent was linalool. The ability for scavenging ABTS•+ and peroxyl free radicals as well as for preventing the growth of THP-1 leukemia cells was better in essential oils with the highest contents of thymol and carvacrol. These results show the importance of these two terpene-phenolic compounds as antioxidants and cytotoxic agents against THP-1 cells.

  7. Assessment of Injuries Caused by Anastrepha fraterculus (Wied.) (Diptera: Tephritidae) on the Incidence of Bunch Rot Diseases in Table Grape.

    Science.gov (United States)

    Machota, R; Bortoli, L C; Cavalcanti, F R; Botton, M; Grützmacher, A D

    2016-08-01

    Anastrepha fraterculus (Wied.) is the main insect pest of table grapes (Vitis vinifera) in the Southern Region of Brazil. In this study, we aimed to investigate the effect of fruit puncturing by adult females and larval infestation by A. fraterculus on the occurrence of bunch rot disease in the grape (cultivar "Itália") by evaluating grapes (a) punctured for oviposition by females of A. fraterculus, sterilized in laboratory with novaluron (40 mg L(-1)) and further spray-inoculated separately with Botrytis cinerea (1 × 10(6) conidia mL(-1)), Glomerella cingulata (1 × 10(6) conidia mL(-1)), and bacteria and yeast that cause sour rot (1 × 10(5) cells mL(-1)), (b) grapes punctured for oviposition by non-sterilized females with pathogen spraying, (c) grapes with mechanical wounds and pathogen spraying, (d) grapes with no wounds and with pathogen spraying, (e) grapes punctured for oviposition by A. fraterculus chemically sterilized in laboratory with novaluron, (f) grapes punctured for oviposition by A. fraterculus non-sterilized in laboratory with novaluron, (g) grapes with mechanical wounds, and (h) grapes with no sterilization or pathogen spraying. Our data indicated that the mechanical and oviposition wounds caused by A. fraterculus increased the percentage of grapes infected by B. cinerea, G. cingulata, and microorganisms of acid rot. The grape puncturing by A. fraterculus and the mechanical wound allows the penetration of B. cinerea and microorganisms leading to acid rot. We conclude that the fruit fly A. fraterculus may facilitate phytopathogens penetration leading to bunch rots in the table grape Itália.

  8. Butia capitata (Mart. Becc. lamina anatomy as a tool for taxonomic distinction from B. odorata (Barb. Rodr. Noblick comb. nov (Arecaceae

    Directory of Open Access Journals (Sweden)

    BRUNO F. SANT'ANNA-SANTOS

    2015-03-01

    Full Text Available The distinction between Butia capitata and B. odorata is based only on a few morphological characteristics, therefore there is a need for additional studies for supporting the separation of the species. As lamina anatomy characteristics are relevant in circumscribing Arecaceae taxa, this work aimed to describe B. capitata lamina anatomy and compare it with B. odorata. Samples from the middle portion of the pinnae were collected and processed in accordance with standard plant anatomy techniques. The epidermis is uniseriate and composed of a thick cuticle and epicuticular waxes into like hooked filaments. The subsidiary cells that arch over the guard cells are located at the hypodermis. The mesophyll is isobilateral and compact. The vascular bundles are collateral with a sclerenchymatic sheath extension that reaches the hypodermis. The stegmata cells have spherical and druse-like silica bodies. The midrib faces the adaxial surface with a thick fibrous layer surrounding the vascular bundles adjacent to the chlorenchyma. The stratified expansion tissue is on the abaxial surface, within the boundary between the mesophyll and midrib. Raphides are only found in B. capitata. Small bundles of the midrib fully surround the fibrous cylinder only in B. odorata. These characteristics are diagnostic and useful for supporting the proposed separation.

  9. Butia capitata (Mart.) Becc. lamina anatomy as a tool for taxonomic distinction from B. odorata (Barb. Rodr.) Noblick comb. nov (Arecaceae).

    Science.gov (United States)

    Sant'anna-Santos, Bruno F; Carvalho Júnior, Wellington G O; Amaral, Vanessa B

    2015-03-01

    The distinction between Butia capitata and B. odorata is based only on a few morphological characteristics, therefore there is a need for additional studies for supporting the separation of the species. As lamina anatomy characteristics are relevant in circumscribing Arecaceae taxa, this work aimed to describe B. capitata lamina anatomy and compare it with B. odorata. Samples from the middle portion of the pinnae were collected and processed in accordance with standard plant anatomy techniques. The epidermis is uniseriate and composed of a thick cuticle and epicuticular waxes into like hooked filaments. The subsidiary cells that arch over the guard cells are located at the hypodermis. The mesophyll is isobilateral and compact. The vascular bundles are collateral with a sclerenchymatic sheath extension that reaches the hypodermis. The stegmata cells have spherical and druse-like silica bodies. The midrib faces the adaxial surface with a thick fibrous layer surrounding the vascular bundles adjacent to the chlorenchyma. The stratified expansion tissue is on the abaxial surface, within the boundary between the mesophyll and midrib. Raphides are only found in B. capitata. Small bundles of the midrib fully surround the fibrous cylinder only in B. odorata. These characteristics are diagnostic and useful for supporting the proposed separation.

  10. Therapieeskalation: Wann und wie?

    Directory of Open Access Journals (Sweden)

    Lutterotti A

    2010-01-01

    Full Text Available Für Patienten mit Multipler Sklerose, für die mit einer immunmodulierenden Basistherapie keine ausreichende Verminderung der Krankheitsaktivität erreicht werden kann, ist eine Eskalationstherapie vorgesehen. Man geht davon aus, dass bei diesen Patienten eine frühe und effektive Intensivierung der Immuntherapie die entzündliche Krankheitsaktivität hemmen kann und die langfristige Prognose günstig beeinflusst. Es stehen verschiedene monoklonale Antikörper und immunsuppressive Substanzen zur Verfügung, allerdings gibt es nur zum Teil gute Evidenz aus kontrollierten Studien. In diesem Übersichtsartikel gehen wir auf die Rationale für den Beginn einer Eskalationstherapie ein und diskutieren die unterschiedlichen Substanzen.

  11. 7 CFR 319.56-31 - Peppers from Spain.

    Science.gov (United States)

    2010-01-01

    ... October 1, and continuing through April 30, MAFF must set and maintain Mediterranean fruit fly (Ceratitis capitata) (Medfly) traps baited with trimedlure inside the greenhouses at a rate of four traps per...

  12. Dicty_cDB: Contig-U11429-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 1 Ceratitis capitata... 40 2e-04 4 ( DJ001102 ) Prediction method of carcinogenicity of chemicals. 46 0.001 ...2 ( DD492826 ) Prediction method of carcinogenicity of chemicals. 46 0.001 2 ( AA

  13. Caracterização molecular de populações de Butia capitata (Arecaceae do Sul do Brasil através de marcadores AFLP Molecular characterization of Butia capitata populations (Arecaceae in Southern Brazil estimated by AFLP analysis

    Directory of Open Access Journals (Sweden)

    Miriam Valli Buttow

    2010-03-01

    Full Text Available O gênero Butia (Arecaceae é um pequeno gênero subtropical com espécies no sul da América do Sul, considerado ornamental. Além disso, seus frutos são apreciados pelo sabor e aroma peculiares. Porém, no Rio Grande do Sul, as populações naturais sofrem com o avanço das atividades rurais e da construção imobiliária. O objetivo deste trabalho foi caracterizar oito populações de Butia capitata ocorrentes no Rio Grande do Sul através de marcadores moleculares do tipo AFLP. Pela análise molecular da variância, foi possível verificar que 83,68% da variabilidade genética são atribuídos à variação entre populações e 13,67% são atribuídos a diferenças entre populações dentro de regiões. A análise comparativa entre as oito populações feita de duas a duas demonstrou que são significativas as diferenças entre 15 populações, com média de 14,72% da variação molecular atribuída às diferenças entre populações. Este resultado indica a presença de variabilidade genética distribuída entre todas as populações, sem subdivisão decorrente de isolamento geográfico.The Butia genus (Arecaceae is a small genus with subtropical species distributed in South America widely used as an ornamental plant. Besides, its fruits are appreciated for its unique flavor and aroma. But in some regions in Rio Grande do Sul State natural populations suffer with progress of building and rural activities. The aim of this study, therefore, was characterizing eight populations of Butia capitata through AFLP markers. By analysis of molecular variance was possible to verify that 83.68% of genetic variability is attributed to variation among populations and 13.67% is attributed to differences among populations within regions. In the pair-wise analysis, differences between 15 populations were significant, with an average of 14.72% of molecular variation attributed to differences between populations. This result indicates the presence of genetic

  14. Dirty Air at the North Pole. Industrial exhaust pollutes the Arctic region; Dicke Luft am Nordpol. Wie Industrieabgase die Arktis verschmutzen

    Energy Technology Data Exchange (ETDEWEB)

    Herber, A. [Alfred-Wegener-Institut fuer Polar- und Meeresforschung (AWI), Bremerhaven (Germany); Lange, G.

    2002-06-01

    After the long polar night, greyish brown haze often hangs over the Arctic landscape. A new 'stellar photometer' which functions also in the dark winter months recently identified its causes. At the North Pole, smog appears to enhance the 'greenhouse effect'. [German] Nach der langen Polarnacht liegen oft graubraune Dunstschwaden ueber der Arktis. Woher kommen und wie entstehen sie? Mit einem neu entwickelten 'Sternfotometer', das auch in den dunklen Wintermonaten Schwebstoffe in der Luft messen kann, liess sich diese Frage jetzt endgueltig klaeren. Anders als in mittleren Breiten scheint der Smog am Nordpol den Treibhauseffekt zu verstaerken. (orig.)

  15. Sexualaufklärung an Oberwalliser Schulen: wie setzen die SIPE-Mitarbeitenden die Sexualaufklärung während der obligatorischen Schulzeit im Oberwallis um?

    OpenAIRE

    Andenmatten, Melanie; Christen, Ursula

    2017-01-01

    Die vorliegende Bachelorarbeit befasst sich mit der Thematik der Sexualaufklärung. Es wird der Frage nachgegangen, wie die Sexualaufklärung von den SIPE-Mitarbeitenden während den obligatorischen Schuljahren im Oberwallis durchgeführt wird. Der theoretische Rahmen widmet sich der Definition von Sexualität und den verschiede-nen Begrifflichkeiten. Wesentliche Fakten bezüglich der Jugendsexualität sowie die Rol-len der Eltern, Lehrpersonen, Fachpersonen und Medien innerhalb der Entwicklung...

  16. O osteocrânio de Proceratophrys boiei (Wied-Neuwied, P. appendiculata (Günther, P. melanopogon (Miranda-Ribeiro e P. laticeps Izecksohn & Peixoto (Anura, Leptodactylidae The osteocranium of Proceratophrys boiei (Wied-Neuwied, P. appendiculata (Günther, P. melanopogon (Miranda-Ribeiro, and P. laticeps Izecksohn & Peixoto, (Anura, Leptodactylidae

    Directory of Open Access Journals (Sweden)

    Eugenio Izecksohn

    2005-03-01

    Full Text Available Foram comparados alguns ossos do crânio de quatro espécies de Proceratophrys Miranda-Ribeiro, 1920 que compartilham longos apêndices palpebrais, como sejam: P. boiei (Wied-Neuwied, 1825, P. appendiculata (Günther, 1873, P. melanopogon (Miranda-Ribeiro, 1926 e P. laticeps Izecksohn & Peixoto, 1981. Com base na forma dos frontoparietais, (com cristas laterais elevadas, na posição dos cantos dos quadratojugais em relação ao plano vertical dos côndilos occipitais, na ornamentação das superfícies cranianas (com tubérculos e grânulos, e na presença de fossetas maxilares posteriores ventrais, P. boiei, P. appendiculata e P. melanopogon foram consideradas em um mesmo grupo. P. laticeps, entretanto, por apresentar arcadas temporais completas (semelhante a Ceratophrys Wied-Neuwied, 1824, cantos dos quadratojugais posteriores ao plano vertical dos côndilos occipitais, superfícies cranianas rugosas e ausência de fossetas maxilares posteriores ventrais, é considerada pertencente a um grupo separado.Skull bones of four Proceratophrys Miranda-Ribeiro, 1920 species sharing long dermal appendages on eyelids - P. boiei (Wied-Neuwied, 1825, P. appendiculata (Günther, 1873, P. melanopogon (Miranda-Ribeiro, 1926, and P. laticeps Izecksohn & Peixoto, 1981 - are herein studied. Proceratophrys boiei, P. appendiculata and P. melanopogon share higher lateral crests on the frontoparietals, quadratojugal corners similarly positioned in relation to the vertical level of the occipital condiles, cranial bones with granules and tubercles, and maxillaries with ventro-posterior pits, hence being considered members of a single group; P. laticeps, given the presence of temporal arcades (similar to Ceratophrys Wied-Neuwied, 1824, quadratojugal corners posterior to the vertical level of occipital condiles, wrinkled skull bones, and maxilaries without ventro-posterior pits, is nonetheless considered as a member of a distinct group.

  17. Avaliação de populações de Butia capitata de Santa Vitória do Palmar Performance of populations of Butia capitata of Santa Vitória do Palmar

    Directory of Open Access Journals (Sweden)

    Elisane Schwartz

    2010-09-01

    Full Text Available O gênero Butia pertence à família Arecaceae e possui cinco espécies com ocorrência no Rio Grande do Sul. A espécie Butia capitata está recebendo atenção especial, não só pelo seu uso no consumo in natura como também em formas processadas. No entanto, mesmo sendo uma espécie que tem sua utilização registrada desde os tempos pré-históricos, vem sendo explorada apenas de modo extrativista, como a maioria das espécies de frutíferas nativas. Além disso, a espécie está seriamente comprometida em médio prazo pela ausência de regeneração natural e com risco muito alto de extinção num futuro próximo. Nesse sentido, o objetivo deste trabalho foi comparar os frutos, a partir de parâmetros químicos e físicos, e também observar os dados produtivos das plantas de três populações de butiazeiros do município de Santa Vitória do Palmar-RS. Os dados foram obtidos em experimentos conduzidos na safra de 2005/2006 e na safra de 2006/2007, em três propriedades localizadas em Santa Vitória do Palmar. Os resultados permitiram verificar que as propriedades e ou variações genéticas entre as populações de Butia capitata avaliadas propiciaram variabilidade para duração do ciclo, coloração da epiderme dos frutos, volume de suco produzido, relação entre sólidos solúveis totais e acidez titulável, características biométricas de fruto e produtividade anual. Uma das populações, denominada Celina, apresentou maior produtividade e rendimento industrial. As populações Celina e São José apresentaram as melhores características biométricas de fruto. A população Aguiar apresentou a melhor relação entre sólidos solúveis totais e acidez titulável.The genus Butia belongs to the Arecaceae family and shows five species occurring in Rio Grande de Sul. The specie Butia capitata requires special attention, not just for its use as fresh consumption but also as processed product. However, even having its use recorded since

  18. Are all eggs created equal? A case study from the Hawaiian reef-building coral Montipora capitata

    Science.gov (United States)

    Padilla-Gamiño, Jacqueline L.; Bidigare, Robert R.; Barshis, Daniel J.; Alamaru, Ada; Hédouin, Laetitia; Hernández-Pech, Xavier; Kandel, Frederique; Leon Soon, Sherril; Roth, Melissa S.; Rodrigues, Lisa J.; Grottoli, Andrea G.; Portocarrero, Claudia; Wagenhauser, Stephanie A.; Buttler, Fenina; Gates, Ruth D.

    2013-03-01

    Parental effects have been largely unexplored in marine organisms and may play a significant role in dictating the phenotypic range of traits in coral offspring, influencing their ability to survive environmental challenges. This study explored parental effects and life-stage differences in the Hawaiian reef-building coral Montipora capitata from different environments by examining the biochemical composition of mature coral colonies and their eggs. Our results indicate that there are large biochemical differences between adults and eggs, with the latter containing higher concentration of lipids (mostly wax esters), ubiquitinated proteins (which may indicate high turnover rate of proteins) and antioxidants (e.g., manganese superoxide dismutase). Adults displayed high phenotypic plasticity, with corals from a high-light environment having more wax esters, lighter tissue δ13C signatures and higher Symbiodinium densities than adults from the low-light environment who had higher content of accessory pigments. A green-algal pigment (α-carotene) and powerful antioxidant was present in eggs; it is unclear whether this pigment is acquired from heterotrophic food sources or from endolithic green algae living in the adult coral skeletons. Despite the broad phenotypic plasticity displayed by adults, parental investment in the context of provisioning of energy reserves and antioxidant defense was the same in eggs from the different sites. Such equality in investment maximizes the capacity of all embryos and larvae to cope with challenging conditions associated with floating at the surface and to disperse successfully until an appropriate habitat for settlement is found.

  19. Molecular cloning and primary sequence analysis of a gene encoding a putative shitinase gene in Brassica oleracea var.capitata

    Institute of Scientific and Technical Information of China (English)

    TANGGUOQING; YONGYANBAI; 等

    1996-01-01

    Chitinase,which catalyzes the hydrolysis of the β-1,4-acetyl-D-glucosamine linkages of the fungal cell wall polymer chitin,is involved in inducible plants defense system.By construction of cabbage(Brassica oleracea var. capitata) genomic library and screening the library with pRCH8,a probe of rice chitinase gene fragment,a chitinase genomic sequence was isolated.The complete uncleotide sequence of the putative cabbage chitinase gene (cabch29) was determined,with its longest open reading frame (ORF) encoding a polypeptide of 413 aa.This polypeptide consists of a 21 aa N-terminal signal peptide,two chitin-binding domains different from those of other classes of plant chitinases,and a catalytic domain.Homology analysis illustrated that this cabch29 gene has 58.8% identity at the nucleotide level with the pRCH8 ORF probe and has 50% identity at the amino acid level tiwh the catalytic domains of chitinase from bean,maize and sugar beet.Meanwhile,several kinds of cis-elements,such as TATA box,CAAT box,GATA motif,ASF-1 binding site,wound-response elements and AATAAA,have also been discovered in the flanking region of cabch29 gene.

  20. Discovery of the chitinozoans Belonechitina capitata from the Shiala Formation of northeastern Garhwal-Kumaon Tethys Himalaya, Pithoragarh District, Uttrakhand, India

    Institute of Scientific and Technical Information of China (English)

    H.N. Sinha; Jacques Verniers

    2016-01-01

    Belonechitina capitata, a typically middle to late Ordovician chitinozoan index taxon was for the first time recovered from the northeastern Kumaon region, a part of Garhwal-Kumaon Tethys basin of the Himalaya, India. This species is of great biostratigraphic importance and has already been reported from Avalonia, Baltica and northern Gondwana. The study area was during Ordovician, part of a low-palaeolatitudinal Gondwana region. The vesicles of recovered forms are black and fragmentary. This is principally attributed to intense tectonic activity during the Himalayan orogenic movement which resulted into high thermal alteration. The chitinozoans are found along with melanosclerites.

  1. Vibrio owensii induces the tissue loss disease Montipora white syndrome in the Hawaiian reef coral Montipora capitata.

    Directory of Open Access Journals (Sweden)

    Blake Ushijima

    Full Text Available Incidences of coral disease in the Indo-Pacific are increasing at an alarming rate. In particular, Montipora white syndrome, a tissue-loss disease found on corals throughout the Hawaiian archipelago, has the potential to degrade Hawaii's reefs. To identify the etiologic agent of Montipora white syndrome, bacteria were isolated from a diseased fragment of Montipora capitata and used in a screen for virulent strains. A single isolate, designated strain OCN002, recreated disease signs in 53% of coral fragments in laboratory infection trials when added to a final concentration of 10(7 cells/ml of seawater. In addition to displaying similar signs of disease, diseased coral fragments from the field and those from infection trials both had a dramatic increase in the abundance of associated culturable bacteria, with those of the genus Vibiro well represented. Bacteria isolated from diseased fragments used in infection trails were shown to be descendants of the original OCN002 inocula based on both the presence of a plasmid introduced to genetically tag the strain and the sequence of a region of the OCN002 genome. In contrast, OCN002 was not re-isolated from fragments that were exposed to the strain but did not develop tissue loss. Sequencing of the rrsH gene, metabolic characterization, as well as multilocus sequence analysis indicated that OCN002 is a strain of the recently described species Vibrio owensii. This investigation of Montipora white syndrome recognizes V. owensii OCN002 as the first bacterial coral pathogen identified from Hawaii's reefs and expands the range of bacteria known to cause disease in corals.

  2. An apparent "vital effect" of calcification rate on the Sr/Ca temperature proxy in the reef coral Montipora capitata

    Science.gov (United States)

    Kuffner, Ilsa; Jokiel, Paul L.; Rodgers, Kuulei; Andersson, Andreas; Mackenzie, Fred T.

    2012-01-01

    Measuring the strontium to calcium ratio in coral skeletons reveals information on seawater temperatures during skeletal deposition, but studies have shown additional variables may affect the ratio. Here we measured Sr/Ca in the reef coral, Montipora capitata, grown in six mesocosms continuously supplied with seawater from the adjacent reef flat. Three mesocosms were ambient controls, and three had seawater chemistry simulating "ocean acidification" (OA). We found that Sr/Ca was not affected by the OA treatment, and neither was coral calcification for these small colonies (larger colonies did show an OA effect). The lack of OA effects allowed us to test the hypothesis that coral growth rate can affect Sr/Ca using the natural range in calcification rates of the corals grown at the same temperature. We found that Sr/Ca was inversely related to calcification rate (Sr/Ca = 9.39 - 0.00404 mmol/mol * mg day-1 cm-2, R2 = 0.32). Using a previously published calibration curve for this species, a 22 mg day-1 colony-1 increase in calcification rate introduced a 1°C warmer temperature estimate, with the 27 corals reporting "temperatures" ranging from 24.9 to 28.9, with mean 26.6 ± 0.9°C SD. Our results lend support to hypotheses invoking kinetic processes and growth rate to explain vital effects on Sr/Ca. However, uncertainty in the slope of the regression of Sr/Ca on calcification and a low R-squared value lead us to conclude that Sr/Ca could still be a useful proxy in this species given sufficient replication or by including growth rate in the calibration.

  3. Forschungsdatenmanagement als überregionale Aufgabe der Informationsversorgung - was kann eine Zentrale Fachbibliothek wie ZB MED Leibniz-Informationszentrum Lebenswissenschaften leisten?

    Directory of Open Access Journals (Sweden)

    Birte Lindstädt

    2015-12-01

    Full Text Available Forschungsdaten sind in aller Munde. Soll sich eine überregionale tätige Bibliothek deshalb auf diesem Feld engagieren? Und falls ja, für wen und wie? Auf der Grundlage einer breit angelegten Markt- und Zielgruppenanalyse strebt ZB MED ein zielgruppengerechtes Angebot im Bereich Forschungsdatenmanagement an, das sich in erster Linie an den Bedürfnissen der lebenswissenschaftlichen Fächer ausrichtet. Research Data Management is much talked about at present. Should a national library get involved in this field? If the answer is yes, what exactly should be offered and for whom? Based on a broad analysis of the market and the target groups, ZB MED is developing services in the field of research data management which focus on the special needs of the life sciences.

  4. Chemical Composition, Antioxidant and Antimicrobial Activities of Thymus capitata Essential Oil with Its Preservative Effect against Listeria monocytogenes Inoculated in Minced Beef Meat

    Directory of Open Access Journals (Sweden)

    Nariman El Abed

    2014-01-01

    Full Text Available The chemical composition, antioxidant and antimicrobial activities, and the preservative effect of Thymus capitata essential oil against Listeria monocytogenes inoculated in minced beef meat were evaluated. The essential oil extracted was chemically analyzed by gas chromatography-mass spectrometry. Nineteen components were identified, of which carvacrol represented (88.89% of the oil. The antioxidant activity was assessed in vitro by using both the DPPH and the ABTS assays. The findings showed that the essential oil exhibited high antioxidant activity, which was comparable to the reference standards (BHT and ascorbic acid with IC50 values of 44.16 and 0.463 μg/mL determined by the free-radical scavenging DPPH and ABTS assays, respectively. Furthermore, the essential oil was evaluated for its antimicrobial activity using disc agar diffusion and microdilution methods. The results demonstrated that the zone of inhibition varied from moderate to strong (15–80 mm and the minimum inhibition concentration values ranged from 0.32 to 20 mg/mL. In addition, essential oil evaluated in vivo against Listeria monocytogenes showed clear and strong inhibitory effect. The application of 0.25 or 1% (v/w essential oil of T. capitata to minced beef significantly reduced the L. monocytogenes population when compared to those of control samples (P-value  <0.01.

  5. Chemical Composition, Antioxidant and Antimicrobial Activities of Thymus capitata Essential Oil with Its Preservative Effect against Listeria monocytogenes Inoculated in Minced Beef Meat.

    Science.gov (United States)

    El Abed, Nariman; Kaabi, Belhassen; Smaali, Mohamed Issam; Chabbouh, Meriem; Habibi, Kamel; Mejri, Mondher; Marzouki, Mohamed Nejib; Ben Hadj Ahmed, Sami

    2014-01-01

    The chemical composition, antioxidant and antimicrobial activities, and the preservative effect of Thymus capitata essential oil against Listeria monocytogenes inoculated in minced beef meat were evaluated. The essential oil extracted was chemically analyzed by gas chromatography-mass spectrometry. Nineteen components were identified, of which carvacrol represented (88.89%) of the oil. The antioxidant activity was assessed in vitro by using both the DPPH and the ABTS assays. The findings showed that the essential oil exhibited high antioxidant activity, which was comparable to the reference standards (BHT and ascorbic acid) with IC50 values of 44.16 and 0.463 μ g/mL determined by the free-radical scavenging DPPH and ABTS assays, respectively. Furthermore, the essential oil was evaluated for its antimicrobial activity using disc agar diffusion and microdilution methods. The results demonstrated that the zone of inhibition varied from moderate to strong (15-80 mm) and the minimum inhibition concentration values ranged from 0.32 to 20 mg/mL. In addition, essential oil evaluated in vivo against Listeria monocytogenes showed clear and strong inhibitory effect. The application of 0.25 or 1% (v/w) essential oil of T. capitata to minced beef significantly reduced the L. monocytogenes population when compared to those of control samples (P-value  <0.01).

  6. PHYSICAL AND CHEMICAL TREATMENTS FOR OVERCOMING DORMANCY IN Butia capitata (MARTIUS BECCARI SEEDS TRATAMENTOS FÍSICOS E QUÍMICOS PARA SUPERAÇÃO DE DORMÊNCIA EM SEMENTES DE Butia capitata (MARTIUS BECCARI

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    Delacyr da Silva Brandão Júnior

    2011-01-01

    Full Text Available The aim of this study was to assess the effect of pre-germination treatments for overcoming dormancy in Butia capitata seeds. The experiment was conducted in a randomized blocks design, in a 3x3 factorial scheme (mechanical treatments x immersion substances, with 20 seeds per plot and four replications. For mechanical treatments, seeds with endocarp (pyrene, seeds without endocarp, and scarified pyrenes were used, whereas, for the immersion substances, gibberellic acid, water, and the control (without immersion were used. The emergence of seedlings began within 51 days, in pyrenes scarified and treated with gibberellic acid. The difference between the first and last count was 190 days. There was no interaction effect for the characteristics assessed, except for the mechanical treatments. It was possible to conclude that the scarified pyrenes showed a higher performance for most  characteristics assessed: emergence percentage, emergence speed index, length, and root fresh and dry mass.

    KEY-WORDS: Arecaceae; pyrene; scarification; gibberellins.Objetivou-se avaliar o efeito de tratamentos pré-germinativos, na superação de dormência em sementes de coquinho-azedo. O experimento foi montado em delineamento de blocos ao acaso, em esquema fatorial 3x3 (tratamentos mecânicos x substâncias de imersão, com 20 sementes por parcela e quatro repetições. Nos tratamentos mecânicos, utilizaram-se sementes com endocarpo (pirênio, sementes sem endocarpo e pirênios escarificados, enquanto, nos tratamentos de substância de imersão, foram utilizados ácido giberélico, água e a testemunha (sem imersão. A emergência das plântulas iniciou-se aos 51 dias, em pirênios escarificados e tratados com ácido giberélico. A diferença entre a primeira e a última contagem foi de 190 dias. Para as características avaliadas, não houve efeito da interação, somente dos tratamentos mecânicos. Concluiu-se que os pirênios escarificados

  7. Eficácia de atrativos alimentares na captura de moscas-das-frutas em pomar de citros Attractiveness of food baits to the fruit flies in citrus orchard

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    Adalton Raga

    2006-01-01

    Full Text Available As moscas-das-frutas (Diptera: Tephritoidea são importantes pragas de frutas cítricas no Estado de São Paulo, principalmente Anastrepha fraterculus (Wied. e Ceratitis capitata (Wied.. O objetivo deste estudo foi medir a eficiência dos atrativos alimentares para monitoramento de moscas-das-frutas, em pomar de citros. O delineamento experimental adotado foi de blocos ao acaso com cinco tratamentos e cinco repetições. O ensaio foi instalado em 30/9/2003 e desenvolvidopor nove semanas. Os atrativos testados foram: proteínas hidrolisadas comerciais Bio Anastrepha, Isca Mosca e Aumax®, todas diluídas em água a 5%; melaço a 7% isoladamente e melaço a 7% em mistura com suco de laranja a 10%. Foram utilizados semanalmente, 400mL da solução atrativa por frasco plástico McPhail instalado na copa de laranjeiras da variedade 'Pêra Rio'. As soluções eram renovadas semanalmente, ocasião em que os adultos eram coletados e çevados ao laboratório para contagem, sexagem e identificação. Foram capturados 1.821 adultos de Tephritoidea, sendo 892 de Anastrepha spp., 731 de C. capitata e 198 de Neosilba spp. Para essas espécies, as proteínas Bio Anastrepha e Isca Mosca foram altamente eficientes e semelhantes entre si, capturando 44,5% e 41,3% dos adultos de Tephritidae respectivamente. Não houve diferença entre as espécies de Tephritidae analisadas por sexo capturadas em Bio Anastrepha, Aumax® e melaço. Aumax®, melaço isoladamente e melaço + suco de laranja atraíram apenas 4,6%, 1,3% e 1,2% do total de adultos de Tephritidae, respectivamente. Melaço isoladamente e melaço com suco de laranja foram mais eficazes na atratividade de Zaprionus indianus (Gupta (Diptera: Drosophilidae.Fruit flies (Diptera: Tephritoidea are key pests in citrus production in the State of São Paulo, Brazil, mainly Anastrepha fraterculus (Wied. and Ceratitis capitata (Wied.. The objective of this study was to evaluate food baits to monitor fruit fly

  8. Experimental Air Warming of a Stylosanthes capitata, Vogel Dominated Tropical Pasture Affects Soil Respiration and Nitrogen Dynamics

    Science.gov (United States)

    Gonzalez-Meler, Miquel A.; Silva, Lais B. C.; Dias-De-Oliveira, Eduardo; Flower, Charles E.; Martinez, Carlos A.

    2017-01-01

    Warming due to global climate change is predicted to reach 2°C in tropical latitudes. There is an alarming paucity of information regarding the effects of air temperature on tropical agroecosystems, including foraging pastures. Here, we investigated the effects of a 2°C increase in air temperature over ambient for 30 days on an established tropical pasture (Ribeirão Preto, São Paulo, Brazil) dominated by the legume Stylosanthes capitata Vogel, using a T-FACE (temperature free-air controlled enhancement) system. We tested the effects of air warming on soil properties [carbon (C), nitrogen (N), and their stable isotopic levels (δ13C and δ15N), as well as soil respiration and soil enzymatic activity] and aboveground characteristics (foliar C, N, δ13C, δ15N, leaf area index, and aboveground biomass) under field conditions. Results show that experimental air warming moderately increased soil respiration rates compared to ambient temperature. Soil respiration was positively correlated with soil temperature and moisture during mid-day (when soil respiration was at its highest) but not at dusk. Foliar δ13C were not different between control and elevated temperature treatments, indicating that plants grown in warmed plots did not show the obvious signs of water stress often seen in warming experiments. The 15N isotopic composition of leaves from plants grown at elevated temperature was lower than in ambient plants, suggesting perhaps a higher proportion of N-fixation contributing to tissue N in warmed plants when compared to ambient ones. Soil microbial enzymatic activity decreased in response to the air warming treatment, suggesting a slower decomposition of organic matter under elevated air temperature conditions. Decreased soil enzyme capacity and increases in soil respiration and plant biomass in plots exposed to high temperature suggest that increased root activity may have caused the increase seen in soil respiration in this tropical pasture. This response

  9. Diet and feeding behavior of the White-naped Jay, Cyanocorax cyanopogon (Wied, 1821 (Aves, Passeriformes, Corvidae in a disturbed environment in central Brazil

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    RAM Barros

    Full Text Available The White-naped Jay Cyanocorax cyanopogon (Wied, 1821 is an omnivorous and opportunistic species, with a multifarious diet. In view of the scarcity of available data in the literature, the aim was to investigate and describe the bird's diet, location of food items, foraging tactics, actual feeding behavior and intraspecific interactions, as a means of defining the items consumed. The study was carried out in a Cerrado area in the Araguaia Campus of the Federal University of Mato Grosso – UFMT, in Pontal do Araguaia, Mato Grosso State, Brazil, from August to December, 2006, and from April to October, 2010. All the feeding events were recorded through 136 hours of animal focal sampling, whereby it was shown that these birds predominantly consume animal nutrients, replenished by vegetable items and human food-waste. Arthropods were predominant in the diet, with ants as the most abundant and frequent item. The fruits, flowers and seeds of eleven plant species were also consumed. Food-waste, representing about 1/10 of the total, was constantly consumed even when other food sources were available. Although active among the various strata, foraging is mainly on the ground. In the event of food-scarcity, the strategy employed is the hierarchical deployment of the members of various-sized groups, with the avoidance of direct competition. This versatility during all seasons, confirms total adaptation to the anthropic environment surrounded by native habitats that characterized the study site.

  10. Diet and feeding behavior of the White-naped Jay, Cyanocorax cyanopogon (Wied, 1821) (Aves, Passeriformes, Corvidae) in a disturbed environment in central Brazil.

    Science.gov (United States)

    Barros, R A M; Costa, C A; Pascotto, M C

    2014-11-01

    The White-naped Jay Cyanocorax cyanopogon (Wied, 1821) is an omnivorous and opportunistic species, with a multifarious diet. In view of the scarcity of available data in the literature, the aim was to investigate and describe the bird's diet, location of food items, foraging tactics, actual feeding behavior and intraspecific interactions, as a means of defining the items consumed. The study was carried out in a Cerrado area in the Araguaia Campus of the Federal University of Mato Grosso - UFMT, in Pontal do Araguaia, Mato Grosso State, Brazil, from August to December, 2006, and from April to October, 2010. All the feeding events were recorded through 136 hours of animal focal sampling, whereby it was shown that these birds predominantly consume animal nutrients, replenished by vegetable items and human food-waste. Arthropods were predominant in the diet, with ants as the most abundant and frequent item. The fruits, flowers and seeds of eleven plant species were also consumed. Food-waste, representing about 1/10 of the total, was constantly consumed even when other food sources were available. Although active among the various strata, foraging is mainly on the ground. In the event of food-scarcity, the strategy employed is the hierarchical deployment of the members of various-sized groups, with the avoidance of direct competition. This versatility during all seasons, confirms total adaptation to the anthropic environment surrounded by native habitats that characterized the study site.

  11. Combining field phenological observations with distribution data to model the potential distribution of the fruit fly Ceratitis rosa Karsch (Diptera: Tephritidae).

    Science.gov (United States)

    de Villiers, M; Hattingh, V; Kriticos, D J

    2013-02-01

    Despite the potential for phenological and abundance data to improve the reliability of species niche models, they are seldom used. The aim of this study was to combine information on the distribution, relative abundance and seasonal phenology of Natal fruit fly, Ceratitis rosa Karsch (Diptera: Tephritidae), in South Africa to model its potential global distribution. Bucket traps, baited with Biolure, were used to trap C. rosa in different climatic regions of South Africa over a two-year period. A CLIMEX niche model of the potential global distribution of C. rosa was fitted using the collected trapping data and other distribution records from South Africa. Independent distribution records for elsewhere in Africa were reserved for model validation. The CLIMEX model results conformed well to the South African trapping data, including information on relative abundance and seasonal phenology, as well as to the pattern of presence records of the species elsewhere in Africa. The model suggests that under recent historical conditions a large part of South America, Central America, Mexico and southern USA may be climatically suitable for establishment of C. rosa. In Europe, climatically suitable habitat is restricted to coastal regions of the Mediterranean, in Asia, mostly to the southern and south eastern countries, and in Australia mostly to the wetter south and east. The independent cross-validation provided by South African relative abundance and seasonal phenology data, central African distribution data and relevant species specific biological information provides greater confidence in the modelled potential distribution of C. rosa.

  12. Significant performance enhancement of yttrium-doped barium cerate proton conductor as electrolyte for solid oxide fuel cells through a Pd ingress-egress approach

    Science.gov (United States)

    Liu, Yu; Ran, Ran; Li, Sidian; Jiao, Yong; Tade, Moses O.; Shao, Zongping

    2014-07-01

    Proton-conducting perovskite oxides are excellent electrolyte materials for SOFCs that may improve power density at reduced temperatures and increase fuel efficiency, thus encouraging the widespread implementation of this attractive technology. The main challenges in the application of these oxides in SOFCs are difficult sintering and insufficient conductivity in real cells. In this study, we propose a novel method to significantly enhance the performance of a yttrium-doped barium cerate proton conductor as an electrolyte for SOFCs through a Pd ingress-egress approach to the development of BaCe0.8Y0.1Pd0.1O3-δ (BCYP10). The capability of the Pd egress from the BCYP10 perovskite lattice is demonstrated by H2-TPR, XRD, EDX mapping of STEM and XPS. Significant improvement in the sinterability is observed after the introduction of Pd due to the increased ionic conductivity and the sintering aid effect of egressed Pd. The formation of a B-site cation defect structure after Pd egress and the consequent modification of perovskite grain boundaries with Pd nanoparticles leads to a proton conductivity of BCYP10 that is approximately 3 times higher than that of BCY under a reducing atmosphere. A single cell with a thin film BCYP10 electrolyte reaches a peak power density as high as 645 mA cm-2 at 700 °C.

  13. 结球甘蓝减数分裂中染色体异常行为分析%Analysis on Chromosome Abnormal Behavior in Meiosis of Brassica oleraces var. Capitata L.

    Institute of Scientific and Technical Information of China (English)

    邵治亮

    2009-01-01

    对300多个纯合二倍体结球甘蓝花粉母细胞减数分裂各时期的染色体进行观察,发现自然条件下纯合二倍体甘蓝花粉母细胞在减数分裂的双线期、终变期以及中期Ⅰ出现了高频率的1~3个四价体和中期Ⅰ有高频率的1~3对染色体提早分离和滞后分离2种异常现象,探讨了甘蓝结实率不高与染色体异常行为之间的关系.%The chromosomes from more than 300 pollen mother cells of homozygous diploid of Brassica oleraces var. Capitata L were observed in every period of meiosis. It was found that altofrequent 1-3 quadrivalent appeared in diplotene, diakinesis and metaphase I of meiosis of pollen mother cells of B. oleraces var. Capitata under natural conditions, and 2 kinds of abnormal phenomenas such as altofrequent 1-3 pairs of chromosomes separated early or late in metaphase I of meiosis. The relationship between low seed setting rate of B. oleraces var. Capitata and chromosome abnormal behavior was explored.

  14. Wie wissenschaftlich ist der Evolutionsgedanke?

    Science.gov (United States)

    Vollmer, Gerhard

    Darwin war ein besonnener Mann; alles Aufsehen war ihm zuwider. Trotzdem hat er eine Revolution ausgelöst, deren Wirkung nicht auf die Biologie beschränkt blieb. Seine Theorie lässt sich in fünf Teiltheorien zerlegen, die sich durch die Begriffe Artenwandel, Verwandtschaft alles Lebendigen und gemeinsamer Ursprung, Artenaufspaltung und Artenvielfalt, Gradualismus, natürliche Auslese charakterisieren lassen. Dadurch wurden mehrere religiöse und weitere weltanschauliche Überzeugungen in Frage gestellt. Deshalb wird die Evolutionstheorie auch heute noch vielfach kritisiert, ja bekämpft. Die Vorwürfe lassen sich ordnen nach den Kriterien, mit denen wir erfahrungswissenschaftliche Theorien beurteilen. Haltbar ist daran nur, dass es für die Evolutionstheorie zwar beliebig viele Bestätigungen gibt, aber nur wenige Widerlegungsmöglichkeiten. Durch die neuerdings entwickelten und durchgeführten Evolutionsexperimente ist die empirische Situation allerdings deutlich besser geworden. Am (erfahrungs)wissenschaftlichen Charakter der Evolutionstheorie besteht deshalb kein Zweifel.

  15. Cold Tolerance of the Mediterranean Fruit Fly in Date and Mandarin.

    Science.gov (United States)

    Gazit, Yoav; Akiva, Ruti; Gavriel, Sagi

    2014-10-01

    The Mediterranean fruit fly, Ceratitis capitata (Wiedemann), is an endemic pest in Israel and there can be low levels of infestation of dates (Phoenix dactylifera L.). Because C. capitata is considered a quarantine pest by several major importing countries, the export of fresh dates requires the elimination of this pest. For mandarin, cold storage at 1.11°C for 15 d is considered to be an effective treatment for the elimination of C. capitata. In this study, we compared the cold tolerance of C. capitata in "Barhi" dates to that of C. capitata in mandarins (Citrus unshiu Marcovitch, "Satsuma"). In Barhi dates, we found the third instars to be the most cold-tolerant as compared with other life stages. Ceratitis capitata in date fruits were significantly less cold-tolerant than C. capitata in Satsuma mandarins. The last viable larvae in dates and mandarins were found after 8 and 13 d of treatment, respectively, and the calculated mortality curves in the two crops were significantly different. These results demonstrate that C. capitata is more sensitive to cold treatment when in date fruits than in mandarins. Therefore, the quarantine cold treatments used to eliminate C. capitata from mandarins should be sufficiently effective if applied to fresh date fruits.

  16. Production of dry matter of Stylosanthes capitata submitted to phosphorus in soil with different textures Produção de matéria seca de Stylosanthes capitata submetido à adubação fosfatada em solos com diferentes texturas

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    Victor Hugo Nakase Otsubo

    2011-12-01

    Full Text Available The low availability of phosphorus is among the factors that limit the development of the Stylosanthes. Only with the appropriate management aiming to increase the phosphorus liberation in the soil we can provide the increase in yield of this leguminous plant. The study was conducted in a greenhouse at the University of Londrina (UEL, the objective was to evaluate the effect of P shoots of Stylosanthes capitata in soil with sandy texture (Typical Yellow Dystropic – LVAd and clay (Typical Eutrophic – Lvef. The experimental design used was completely randomized with four replications in a 2x5x2 factorial design, in which the factors were two soil types, five shots of phosphorus (0, 40, 80, 120 and 160 kg ha-1 of P2O5 and two cuts. For the sowing, seeds of Stylosanthes capitata were used. The aerial part’s cuts were made at 60 and 105 days after sowing. Regardless of the texture and cut, the phosphated fertilization increased the dry matter production of Stylosanthes’ aerial part, and the maximum yield was obtained with a shot of 105 kg ha-1 P2O5. Among the soils the biggest production was obtained in the second cut and soil of sandy texture. With the increase of the doses, the phosphorus content increases in dry matter yield, improving their nutritional quality for animal feed. The maximum dry matter production (2.39 g / plant in sandy soil and 1.36 g / plant in clay soil were obtained with doses of 102.69 and 113.25 kg ha-1 P2O5, respectively.A baixa disponibilidade de fósforo (P está entre os fatores que limitam o desenvolvimento do estilosante. Somente com manejo adequado que vise aumentar a liberação do fósforo no solo se consegue altos rendimentos desta leguminosa. O trabalho foi desenvolvido em casa de vegetação na Universidade Estadual de Londrina (UEL, com o objetivo de avaliar o efeito de doses de P na produção de matéria seca de Stylosanthes capitata em solos com texturas arenosa (Latossolo Vermelho-Amarelo Distr

  17. A Co-Dominant Marker BoE332 Applied to Marker-Assisted Selection of Homozygous Male-Sterile Plants in Cabbage (Brassica oleracea var. capitata L.)

    Institute of Scientific and Technical Information of China (English)

    CHEN Chen; ZHUANG Mu; FANG Zhi-yuan; WANG Qing-biao; ZHANG Yang-yong; LIU Yu-mei; YANG Li-mei; CHENG Fei

    2013-01-01

    The dominant genic male sterility (DGMS) gene CDMs399-3 derived from a spontaneous mutation in the line 79-399-3 of spring cabbage (Brassica oleracea var. capitata L.), has been successfully applied in hybrid seed production of several cabbage cultivars in China. During the development of dominant male sterility lines in cabbage, the conventional identification of homozygous male-sterile plants (CDMs399-3/CDMs399-3) is a laborious and time-consuming process. For marker-assisted selection (MAS) of the gene CDMs399-3 transferred into key spring cabbage line 397, expressed sequence tag-simple sequence repeats (EST-SSR) and SSR technology were used to identify markers that were linked to CDMs399-3 based on method of bulked segregant analysis (BSA). By screening a set of 978 EST-SSRs and 395 SSRs, a marker BoE332 linked to the CDMs399-3 at a distance of 3.6 cM in the genetic background of cabbage line 397 were identified. 7 homozygous male-sterile plants in population P1170 with 20 plants were obtained finally via MAS of BoE332. Thus, BoE332 will greatly facilitate the transferring of the gene CDMs399-3 into the key spring cabbage line 397 and improve the application of DGMS in cabbage hybrid breeding.

  18. Nondestructive Optical Sensing of Flavonols and Chlorophyll in White Head Cabbage (Brassica oleracea L. var. capitata subvar. alba) Grown under Different Nitrogen Regimens.

    Science.gov (United States)

    Agati, Giovanni; Tuccio, Lorenza; Kusznierewicz, Barbara; Chmiel, Tomasz; Bartoszek, Agnieszka; Kowalski, Artur; Grzegorzewska, Maria; Kosson, Ryszard; Kaniszewski, Stanislaw

    2016-01-13

    A multiparametric optical sensor was used to nondestructively estimate phytochemical compounds in white cabbage leaves directly in the field. An experimental site of 1980 white cabbages (Brassica oleracea L. var. capitata subvar. alba), under different nitrogen (N) treatments, was mapped by measuring leaf transmittance and chlorophyll fluorescence screening in one leaf/cabbage head. The provided indices of flavonols (FLAV) and chlorophyll (CHL) displayed the opposite response to applied N rates, decreasing and increasing, respectively. The combined nitrogen balance index (NBI = CHL/FLAV) calculated was able to discriminate all of the plots under four N regimens (0, 100, 200, and 400 kg/ha) and was correlated with the leaf N content determined destructively. CHL and FLAV were properly calibrated against chlorophyll (R(2) = 0.945) and flavonol (R(2) = 0.932) leaf contents, respectively, by using a homographic fit function. The proposed optical sensing of cabbage crops can be used to estimate the N status of plants and perform precision fertilization to maintain acceptable crop yield levels and, additionally, to rapidly detect health-promoting flavonol antioxidants in Brassica plants.

  19. Wie das Neue in eine Brandenburgische Hochschulbibliothek kommt und nicht versiegen mag – Ein persönlicher Rückblick

    Directory of Open Access Journals (Sweden)

    Frank Seeliger

    2015-11-01

    Full Text Available Das Mantra der Kundenorientierung, Serviceentwicklung und die Verpflichtung zur Effektivität wie Effizienz bei der Bewältigung aller Geschäftsgänge sind mittlerweile Allgemeinplätze. Es verpflichtet Informationseinrichtungen, den aktuellen Entwicklungen und der Zeit auf den Fersen zu bleiben. Was bedeutet dies auf der alltäglich pragmatischen Ebene für eine überschaubare Fachhochschulbibliothek? Eine, die mit wenigen Ressourcen ausgestattet als Showcase, repräsentativer Raum, Boutique sich gut „verkaufen“ muss, um en vogue oder einfach nur relevant und interessant für Stakeholder, Nutzer sowie Mitarbeiter zu bleiben? Auf dem Campus der berlinnahen Informationseinrichtung wird seit dem Neubezug einer denkmalgeschützten Verladehalle 2007 versucht, dem gewinnenden Charme und anmutigen Ambiente des Porticus auch eine herausragende Rolle als Innovations-Hub für die Hochschule und Bibliotheken im Allgmeinen über gelebte neue Ideen abzugewinnen. Sie betreffen sowohl technische Lösungen in Bezug auf RFID, Visualisierung, Automatisierung und Software, aber auch Formate für Veranstaltungen und Weiterbildung. Was ist aber hierfür nötig, um das Neue, gekauft oder selbst gezimmert, immer wieder einfliessen zu lassen und sich mit dem Profil der tatkräftigen und kreativen Avantgarde zu identifizieren? Der Beitrag wird versuchen, das Umfeld dafür zu beleuchten, um Innovationen als selbstverständliche Erwartungshaltung reifen zu lassen. Der deskriptive Ansatz soll nach keineswegs vollständiger Reflexion eigener Bemühungen prima facie Anregung geben dazu, wie im öffentlichen Dienst und ohne direkte Tuchfühlung zu „schwarzen Zahlen“ eine Firmenkultur Einzug halten kann, die bei entsprechender Erwartungshaltung das Unvorhersehbare für sich höher einschätzt als das Vertraute. Es ist ein Plädoyer für das offene System, ein Hoch auf Veränderung und Neues, ohne damit in eine willkürliche Vielfalt zu geraten. Warum kann das

  20. Feeding ecology of Tropidurus torquatus (Wied (Squamata, Tropiduridae in two areas with different degrees of conservation in Marambaia Island, Rio de Janeiro, Southeastern Brazil Ecologia alimentar de Tropidurus torquatus (Wied (Sauria, Tropiduridae em duas áreas com diferentes níveis de conservação na Ilha da Marambaia, Rio de Janeiro, Sudeste do Brasil

    Directory of Open Access Journals (Sweden)

    André L. G. de Carvalho

    2007-03-01

    Full Text Available We compared the effects of habitat disturbance on the feeding ecology of two local populations of Tropidurus torquatus (Wied, 1820 in restinga habitats on Ilha da Marambaia, Southeastern Brazil. Both populations presented a relatively diverse diet and differ in the number (D Max = 0.22, DF = 2, p = 0.00 and volume of prey consumed (D Max = 0.82, DF = 2, p = 0.00. Ants were the most frequent and numerous preys in both areas, and plant matter and coleopterans were the most important alimentary items in volume. The population from the disturbed area presented a larger niche breadth for number of prey items consumed (Bi num = 3.06 and volume (Bi vol = 2.98, when compared to the other population (Bj num = 2.44, Bj vol = 1.52. Niche overlap was higher for number of items consumed between the populations (Oij num = 0.82 but lower for volume (Oij vol = 0.05. Our data suggest dietary differences between the two lizard populations, and these differences may be associated with environmental modifications. Our main hypothesis to explain the dietary differences is based in the Optimal Foraging Theory. However, the alimentary behavior, and consequently the diet of these lizards, seems to be constrained phylogenetically, with a pattern that may have evolved in the ancestor of all iguanids.Comparamos os efeitos da degradação ambiental sobre a ecologia alimentar de duas populações de Tropidurus torquatus (Wied, 1820 em áreas de restinga da Ilha da Marambaia, sudeste do Brasil. Ambas as populações apresentaram dieta relativamente diversa e deferiram em número (D Max = 0,22; DF = 2; p = 0,00 e volume de presas consumidas (D Max = 0,82; DF = 2; p = 0,00. Formigas foram os itens mais freqüentes e numerosos em ambas as áreas, e material vegetal e coleópteros os mais importantes em volume. A população da área perturbada apresentou maior largura de nicho alimentar para número de presas consumidas (Bi num = 3,06 e volume (Bi vol = 2,98, quando comparada

  1. El modelo V.S.P. en lechuga Batavia Lactuca sativa var. Capitata L. y respuesta de dos variedades a las aplicaciones de compuestos orgánicos

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    Bruzón C. Serapio F.

    1996-12-01

    Full Text Available

    An investigation was realized in the region of Pavitas, municipality of La Cumbre, Santa Fe farm, 1480 meters above sea level in a soil of Pavas Association (Aeric Tropaquept to prove the plastic cup-sustrate-plant model in the production of Batavia lecttuce Lactuca sativa var. capitata, and the effects of the organic matter filter cake press, cattle manure, poultry manure and earthworm soil, incorporated in the soil. Twenty two (22 treatments and fours repetitions were applied with each of the two varieties (Great lakes 118 and Pacific, the treatments corresponded to 5, 10, 15 t/ha of the organic matter which were applied to the soil fifteen days before transplantations occured. The results showed an excellent plant vigour and quality after the transplantation. It was also observed a good plant poblation and uniformity. The sustrate filter cake press mixed with botton fly ash presented better results in the field than the other treatments, having the highest crop yield and the best quality. The Great Lakes 118 with this treatment doubled the production of the treatment with soil. With the organic sustrates, the poultry manure 5 t/ha projected better results. The work showed the good behavior of the varity Great Lakes 118 compared to the Pacific. This treatments also improved the chemical caracteristics of the soil, especially increased the organic matter, potassium, phosporus, manganesum and zinc.

    En el Corregimiento Pavitas, municipio de La Cumbre, en la Finca Santafé, a 1.480 msnm en un suelo de la Asociación Pavas (Aeric Tropaquept se probó el modelo vaso-sustrato-planta (VSP, en la producción de plántulas de lechuga Batavia L. sativa var. capitata y el efecto en la producción de los compuestos orgánicos, cachaza, lombricompuesto, gallinaza y bovinaza, incorporados al suelo. Los 22 tratamientos con cuatro repeticiones, repartidos en dos variedades (Great Lake 118 y Pacific correspondieron a dosis (5, 10, 15 t/ha de las

  2. Chromosome Doubling of Microspore-Derived Plants from Cabbage (Brassica oleracea var. capitata L.) and Broccoli (Brassica oleracea var. italica L.).

    Science.gov (United States)

    Yuan, Suxia; Su, Yanbin; Liu, Yumei; Li, Zhansheng; Fang, Zhiyuan; Yang, Limei; Zhuang, Mu; Zhang, Yangyong; Lv, Honghao; Sun, Peitian

    2015-01-01

    Chromosome doubling of microspore-derived plants is an important factor in the practical application of microspore culture technology because breeding programs require a large number of genetically stable, homozygous doubled haploid plants with a high level of fertility. In the present paper, 29 populations of microspore-derived plantlets from cabbage (Brassica oleracea var. capitata) and broccoli (Brassica oleracea var. italica) were used to study the ploidy level and spontaneous chromosome doubling of these populations, the artificial chromosome doubling induced by colchicine, and the influence of tissue culture duration on the chromosomal ploidy of the microspore-derived regenerants. Spontaneous chromosome doubling occurred randomly and was genotype dependent. In the plant populations derived from microspores, there were haploids, diploids, and even a low frequency of polyploids and mixed-ploidy plantlets. The total spontaneous doubling in the 14 cabbage populations ranged from 0 to 76.9%, compared with 52.2 to 100% in the 15 broccoli populations. To improve the rate of chromosome doubling, an efficient and reliable artificial chromosome doubling protocol (i.e., the immersion of haploid plantlet roots in a colchicine solution) was developed for cabbage and broccoli microspore-derived haploids. The optimal chromosome doubling of the haploids was obtained with a solution of 0.2% colchicine for 9-12 h or 0.4% colchicine for 3-9 h for cabbage and 0.05% colchicine for 6-12 h for broccoli. This protocol produced chromosome doubling in over 50% of the haploid genotypes for most of the populations derived from cabbage and broccoli. Notably, after 1 or more years in tissue culture, the chromosomes of the haploids were doubled, and most of the haploids turned into doubled haploid or mixed-ploidy plants. This is the first report indicating that tissue culture duration can change the chromosomal ploidy of microspore-derived regenerants.

  3. Influence of fermentation conditions on glucosinolates, ascorbigen, and ascorbic acid content in white cabbage (Brassica oleracea var. capitata cv. Taler) cultivated in different seasons.

    Science.gov (United States)

    Martinez-Villaluenga, C; Peñas, E; Frias, J; Ciska, E; Honke, J; Piskula, M K; Kozlowska, H; Vidal-Valverde, C

    2009-01-01

    The content of glucosinolates (GLS), ascorbigen, and ascorbic acid in white cabbage (Brassica oleracea var. capitata cv. Taler) cultivated in different seasons (summer and winter) was determined, before and after spontaneous and starter-induced fermentation. Different salt concentrations (0.5% NaCl or 1.5% NaCl) were used for sauerkraut production. Glucoiberin, sinigrin, and glucobrassicin were dominating in raw white cabbage cultivated either in winter or summer seasons. Ascorbigen precursor, glucobrassicin, was found higher in cabbage cultivated in winter (2.54 micromol/g dw) than those grown in summer (1.83 micromol/g dw). Cabbage fermented for 7 d was found to contain only traces of some GLS irrespective of the fermentation conditions used. Ascorbigen synthesis occurred during white cabbage fermentation. Brining cabbage at low salt concentration (0.5% NaCl) improved ascorbigen content in sauerkraut after 7 d of fermentation at 25 degrees C. The highest ascorbigen concentration was observed in low-sodium (0.5% NaCl) sauerkraut produced from cabbage cultivated in winter submitted to either natural (109.0 micromol/100 g dw) or starter-induced fermentation (108.3 and 104.6 micromol/100 g dw in cabbages fermented by L. plantarum and L. mesenteroides, respectively). Ascorbic acid content was found higher in cabbage cultivated in summer and fermentation process led to significant reductions. Therefore, the selection of cabbages with high glucobrassicin content and the production of low-sodium sauerkrauts may provide enhanced health benefits towards prevention of chronic diseases.

  4. Aroma therapy and medfly SIT

    Energy Technology Data Exchange (ETDEWEB)

    Shelly, Todd E., E-mail: todd.e.shelly@aphis.usda.go [U.S. Department of Agriculture (USDA-APHIS), HI (United States). Animal and Plant Health Inspection

    2006-07-01

    A summary of the main findings of the research program on the biological competence of mass-reared, sterile males of the Mediterranean fruit fly (med fly), Ceratitis capitata (Wied.) and the development and implementation of the sterile insect technique (SIT) against this pest is presented. The potential application of aroma therapy to improve the mating success of sterile med fly males is studied. The report assumes a loosely chronological framework as it documents progression along two experimental scales: the number of males simultaneously exposed to ginger root oil, starting with small groups of 25 males and ending with rooms with nearly 200 million males; the experimental arena used to test the effects of aroma therapy, progressing from standard field-cages to large field enclosures to the open field. In addition, brief comments are offered regarding the potential negative effects of GRO exposure, the mechanisms underlying GRO-mediated improvement in male mating success, and the financial costs of GRO aroma therapy. (MAC)

  5. Fruit flies (Diptera: Tephritidae associated to native fruit of Spondias spp. (Anacardiaceae and Ximenia americana L. (Olacaceae and their parasitoids in the State of Piaui, Brazil

    Directory of Open Access Journals (Sweden)

    Almerinda Amélia Rodrigues Araújo

    2014-09-01

    Full Text Available This work aims to identify the species of fruit flies and their parasitoids associated to native fruit of Spondias spp. (caja S. mombin L., umbu-caja Spondias sp., umbu S. tuberosa Arr. Câm. and wild plum Ximenia americana L., in the State of Piaui, Brazil. Samples (63 of fruits were collected from November 2009 to July 2010, totalizing 4,495 fruits and 46,906 kg. It was possible to obtain 10,617 puparia, from which 4,497 tephritids and 1,118 braconid parasitoids emerged. Regarding Spondias spp., the highest occurrence was Anastrepha obliqua (Macquart, with 100% for umbu and umbu-caja. Caja presented an average of 99.52% of A. obliqua, 0.46% of Anastrepha fraterculus (Wied. and 0.97% of Ceratitis capitata (Wied.. Wild plum percentages were 97.83% for A. alveata Stone and 2.17% for A. fraterculus. Infestation rates were 429.2, 178.4, 158.9 and 43.3 puparia/kg in umbu-caja, caja, wild plum and umbu, respectively. Pupal viability was 77.8%, 69.3%, 52.5% and 41.1% to umbu, wild plum, umbu-caja and caja, respectively. By analyzing the sample parasitoids, the percentage was 21.39% for the Doryctobracon areolatus (Szépligeti species and 78.61% for Opius bellus Gahan. For the first time, it was recorded in Brazil X. americana as a host to A. alveata, as well as D. aleolatus and O. bellus as parasitoids of A. obliqua and A. alveata in Piaui.

  6. Sublethal effect of neem extract on mediterranean fruit fly adults

    Directory of Open Access Journals (Sweden)

    Márcio Alves Silva

    2013-03-01

    Full Text Available The sublethal effect of extracts of Azadirachta indica on Ceratitis capitata was evaluated. Two pairs of flies were treated in plastic tubes with cotton placed in plastic cages. An artificial diet (hydrolyzed protein + sugar was provided ad libitum. The extracts affected significantly the longevity of C. capitata. The pre-oviposition period were not significantly affected by the extracts. The A. indica branches extracted with dichloromethane (888 ppm affected significantly the fecundity and fertility, reducing the number of eggs laid to approximately 80 % and the egg hatching by 30 % at the 8th day. Therefore, the neem branches extracted with dichloromethane affected the reproduction of C. capitata.

  7. Captures of bactrocera fruit flies (Diptera: Tephritidae) and nontarget insects in biolure and torula yeast traps in Hawaii

    Science.gov (United States)

    BioLure, a synthetic food attractant for Mediterranean fruit fly (Ceratitis capitata (Wiedemann)) that uses a combination of three chemical components (ammonium acetate, trimethylamine hydrochloride and putrescine), was deployed in MultiLure traps in predominantly native forests, non-native forests,...

  8. Subtropical Fruit Fly Invasions into Temperate Fruit Fly Territory in California's San Joaquin Valley

    Science.gov (United States)

    Subtropical fruit fly species including peach fruit fly, Bactrocera zonata (Saunders); melon fly, B. cucurbitae (Coquillett); oriental fruit fly, B. dorsalis (Hendel); and Mediterranean fruit fly, Ceratitis capitata Weidemann, have been detected in the past decade in the San Joaquin Valley of Califo...

  9. A qPCR-based method for detecting parasitism of Fopius arisanus (Sonan) in oriental fruit flies, Bactrocera dorsalis (Hendel)

    Science.gov (United States)

    BACKGROUND: Parasitism rate detection and parasitoid species identification are necessary in fruit fly biological control. Currently release of mass-reared Fopius arisanus is occurring world-wide, as this species is effective in controlling Bactrocera dorsalis and Ceratitis capitata. While release i...

  10. Rhythmicity of Bactrocera cucurbitae (Diptera:Tephritidae) attraction to cuelure: Insights from an interruptable lure and computer vision

    Science.gov (United States)

    We describe and validate an Agent-Based Simulation (ABS) of invasive insects and use it to investigate the time to extirpation of Ceratitis capitata using data from seven outbreaks that occurred in California from 2008-2010. Results are compared with the length of intervention and quarantine imposed...

  11. Transcriptome of the egg parasitoid Fopius arisanus, an important biocontrol tool for Tephritid fruit fly suppression

    Science.gov (United States)

    Background The Braconoid wasp Fopius arisanus (Sonan) has been utilized for biological control of the Mediterranean fruit fly (Ceratitis capitata), and the oriental fruit fly (Bactrocera dorsalis), both phytophagous fruit flies pest of economic importance in Hawaii. We have sequenced and assembled t...

  12. Conditional lethality strains for the biological control of Anastrepha species

    Science.gov (United States)

    Pro-apoptotic cell death genes are promising candidates for biologically-based autocidal control of pest insects as demonstrated by tetracycline (tet)-suppressible systems for conditional embryonic lethality in Drosophila melanogaster (Dm) and the medfly, Ceratitis capitata (Cc). However, for medfly...

  13. Efficacy of wax matrix bait stations for Mediterranean Fruit Flies (Diptera: Tephritidae)

    Science.gov (United States)

    Tests were conducted that evaluated efficacy of wax matrix bait stations for Ceratitis capitata (Wiedemann) adults in Guatemala. Bait stations were exposed to outdoor conditions to determine effect of weathering on longevity as indicated by bait station age. Results of laboratory tests found that ba...

  14. Exposure to tea tree oil enhances the mating success of male Mediterranean fruit flies (Diptera: Tephritidae)

    Science.gov (United States)

    The aroma of various plant essential oils has been shown to enhance the mating competitiveness of males of the Mediterranean fruit fly (medfly), Ceratitis capitata (Wiedemann). Laboratory observations revealed that male medflies show strong short-range attraction to tea tree oil (TTO hereafter) deri...

  15. Evidence for the evolutionary nascence of a novel sex determination pathway in honeybees

    DEFF Research Database (Denmark)

    Hasselmann, Martin; Gempe, Tanja; Schiøtt, Morten

    2008-01-01

    -determining gene transformer (tra), but lacks conserved motifs except for a 30-amino-acid motif that Fem shares only with Tra of another fly, Ceratitis capitata. Like tra, the fem transcript is alternatively spliced. The male-specific splice variant contains a premature stop codon and yields no functional product...

  16. Biological and Cultural Control of Olive Fruit Fly in California---Utilization of Parasitoids from USDA-APHIS-PPQ, Guatemala and Cultural Control Methods

    Science.gov (United States)

    The parasitoid Psytallia humilis = P. cf. concolor (Szépligeti) was reared on sterile Mediterranean fruit fly, Ceratitis capitata (Wiedemann), larvae at the USDA, APHIS, PPQ, Moscamed biological control laboratory in San Miguel Petapa, Guatemala and shipped to the USDA, ARS, Parlier, for biological ...

  17. Evidence for potential of managing some african fruit fly species (Diptera: Tephritidae) using the mango fruit fly host-marking pheromone

    Science.gov (United States)

    We investigated conspecific and heterospecific oviposition host discrimination among four economically important fruit fly pests of mango in Africa (Ceratitis capitata, Wiedemann; C. fasciventris, Bezzi; C. rosa, Karsch, and C. cosyra, Walker) with regard to host-marking behavior and fecal matter aq...

  18. Embryogenesis in Culture of Isolated Microspores of Brassica juncea var.capitata%结球芥菜游离小孢子培养的胚状体发生

    Institute of Scientific and Technical Information of China (English)

    王长龙; 杨培新; 郑奕雄

    2011-01-01

    2009~2011年对6种不同结球芥菜品种进行了小孢子培养试验,结果表明:在相同的培养条件下,各品种胚状体的发育是不同步的,胚状体的平均产量也存在极显著的差异,以大平铺的平均产胚量最高,为3.52个/蕾,其次为哥历蕾.%The isolated microspores of 6 Brassica juncea var. Capitata varieties were cultured from 2009 to 2011. The results showed that: under the same culture conditions, the embryogenesis of different varieties was of asynchrony, the average embryoid yield had extremely significant difference among different varieties, and the variety Dapingpu had the highest average embryoid yield (3.52 embryoids per bud) , followed by Gelilei.

  19. Sterilization of fruit flies (Diptera: Tephritidae) with X-rays for sterile insect technique programs; Esterilizacao de moscas-das-frutas (Diptera: Tephritidae) com raios-X para programas de tecnica do inseto esteril

    Energy Technology Data Exchange (ETDEWEB)

    Mastrangelo, Thiago de Araujo

    2009-07-01

    Recent fear of acts of terrorism provoked an increase of delays and denials in the shipment of radioisotopes. This truly represented a menace to sterile insect production projects around the world. In order to validate the use of a new kind of low-energy Xray irradiator, a series of radiobiological studies on Ceratitis capitata (tsl-VIENNA 8 strain) (Wied., 1824) (Diptera: Tephritidae) and an Argentinean strain of Anastrepha fraterculus (Wied., 1830) (Diptera: Tephritidae) were carried out, also comparing biological effectiveness between X-rays and traditional {gamma} radiation from {sup 60}Co. Pupae 48- 24 h before adult emergence of C. capitata males and both sexes of A. fraterculus were irradiated with doses ranging from 15 to 120 Gy and 10 to 70 Gy respectively. Doses that induce 50, 90 and 99% of sterility were estimated and the hypothesis of Parallelism for the Probit equations was tested. Doses of 82.7 Gy of X-rays and 128.2 Gy of {gamma} rays (thus, a RBE{approx}1.5) induced 99% sterility on medfly males. The fertility of A. fraterculus fertile females crossed with 41 Gy of X-rays and 62.7 Gy of {gamma} rays decreased in 99% comparing with the control group (RBE{approx}1.5). 99% sterility of A. fraterculus irradiated females was achieved with 60-80 Gy (RBE{approx}0.7). The standard quality control parameters of fecundity, adult emergence, fliers and survival were not significantly affected by the two types of radiation (RBE{approx}1) either for medfly or A. fraterculus (p>0.01), being averages in conformity with the values required by FAO/IAEA/USDA. Only fecundity of irradiated A. fraterculus females was severely reduced with increasing doses and no egg was laid at 70 Gy of both radiations. There were no significant differences between X-rays and {gamma} rays regarding mating indices (RSI for medfly, RII, ISI, MRPI and FRPI for A. fraterculus) (p>0.05), what indicated more random matings for fertile and sterile insects. The results demonstrated that no

  20. Global CO{sub 2} emissions 2015. Trend reversion is still waiting, despite hopeful approaches; Weltweite CO{sub 2}-Emissionen 2015. Trendwende laesst trotz hoffnungsvoller Ansaetze nach wie vor auf sich warten

    Energy Technology Data Exchange (ETDEWEB)

    Ziesing, Hans-Joachim

    2016-10-15

    By 2015, global CO{sub 2} emissions were virtually unchanged from 2014 according to provisional calculations. On the whole, a slight increase may have resulted, which has slowed the growth of the past few years. In the previous year alone, the weakest growth since the beginning of the century had been recorded, with only 0.7% of the upturn (with the exception of the crises in 2008/2009). As a result, CO{sub 2} emissions in 2015 were only slightly higher than in the previous year, at 33.1 billion tonnes. A global trend reversal is still likely, but the CO{sub 2} emissions have declined in many countries, particularly in some industrialized countries. [German] Im Jahr 2015 haben sich die weltweiten CO{sub 2}-Emissionen nach vorlaeufigen Berechnungen gegenueber 2014 praktisch nicht veraendert. Insgesamt duerfte sich allenfalls ein leichtes Plus ergeben haben, womit der Zuwachs der vergangenen Jahre erneut gebremst wurde. Schon im Vorjahr war mit einem Plus von nur 0,7 % der bis dahin (mit Ausnahme der Krisenjahre 2008/2009) schwaechste Anstieg seit Beginn des Jahrhunderts zu verzeichnen gewesen. Im Ergebnis blieben die CO{sub 2}-Emissionen 2015 mit reichlich 33,1 Mrd. t nur geringfuegig ueber dem Niveau des Vorjahres. Eine weltweite Trendwende steht wohl noch nach wie vor aus, doch sind immerhin die CO{sub 2}-Emissionen in zahlreichen Laendern, insbesondere in etlichen Industrielaendern, gesunken.

  1. Electricity to be treated in the same way as oil. Features of the draft for the first stage of the ecological tax reform; Wie das Oel, so der Strom. Merkmale des Gesetzentwurfs zum Einstieg in die oekologische Steuerreform

    Energy Technology Data Exchange (ETDEWEB)

    Schafhausen, F. [Bundesministerium fuer Umwelt, Naturschutz und Reaktorsicherheit, Bonn (Germany)

    1999-01-01

    No sooner had the coalition talks been concluded and the last words of the new chancellor`s statement of government policy sounded through the plenary hall than the newly elected German Bundestag presented its draft of a tax law ringing in the first round of the ecological tax reform. The basic idea is simple: being a relatively scarce production factor environmental resources are to be taxed more heavily, whereas labour as an abundantly available production factor is to be relieved of charges. This economic impulse is to help spare raw materials, conserve the environment and curb unemployment. [Deutsch] Kaum waren die Koalitionsverhandlungen abgeschlossen und die letzten Worte der Regierungserklaerung durch den neuen Kanzler vorgetragen, wurde aus der Mitte des Deutschen Bundestages der `Entwurf fuer ein Steuergesetz zum Einstieg in die oekologische Steuerreform` vorgelegt. Die Grundidee ist simpel: Der vergleichsweise knappe Produktionsfaktor Umwelt soll belastet werden, waehrend reichlicher vorhandene Produktionsfaktoren wie Arbeit entlastet werden sollen. Dieser oekonomische Impuls soll eine Schonung der Rohstoffe und der Umwelt bewirken und die Arbeitslosigkeit abbauen. (orig.)

  2. Pollutants, mould and cleanliness - hygiene and health hazards in the home. Seminar volume of the Central Information Bureau for Environmental Consulting in Bavaria. Vol. 17; Schadstoffe, Schimmel und Sauberkeit - wie (un)gesund ist unser Zuhause? Seminarband der Zentralen Informationsstelle Umweltberatung Bayern. Bd. 17

    Energy Technology Data Exchange (ETDEWEB)

    Behling, G.; Spoettle, M.; Koller, U. (comps.)

    2001-07-01

    The seminar investigated the technical and hygienic requirements that should be made on constructional materials and interior materials, the potential health hazards involved, and the available alternatives. The following examples were presented: Trends and specifications in low-emission furniture and wood-based materials; Analysis of current flooring materials, glues and sealings; Disinfectants as sources of room air pollution; Potential hazards in domestic hygiene; Analysis of room air pollution levels; Practical consultations in environmental medicine. [German] Welche bautechnischen und innenraumhygienischen Anforderungen sind an Baustoffe und Materialien zu stellen ? Wie beeintraechtigen diese moeglicherweise die menschliche Gesundheit und wie sind oft hochgelobte Alternativen einzuschaetzen? Diese Fragen wurden anhand verschiedener Beispiele geklaert: Entwicklungen und Richtlinien bei emissionsarmen Moebeln und Holzwerkstoffen, Bewertung derzeit aktueller Bodenbelaege, Kleber und Oberflaechenbehandlungen, Quellen fuer eine Raumluftbelastung durch Schaedlingsbekaempfungsmittel, Gefahrenpotenziale in der Haushaltshygiene, Schimmelpilze und moegliche Massnahmen, Analyse der Innenraumluftbelastung, Umweltmedizinische Beratung in der Praxis. (orig.)

  3. Deposição diária e sazonal de gordura subcutânea em Phacellodomus rufifrons (Wied (Aves, Furnariidae Daily and seasonal deposition of subcutaneous fat in Phacellodomus rufifrons, a Neotropical ovenbird

    Directory of Open Access Journals (Sweden)

    Fernando F. Goulart

    2007-01-01

    Full Text Available Aves que habitam regiões temperadas acumulam gordura a fim de sobreviver às condições extremas da noite. Níveis de deposição de gordura nessas aves aumentam ao longo do dia e atingem um pico no entardecer e diminuem ao longo da noite, quando a alimentação não é possível. Aliado a isso, existe o aumento estacional dos depósitos de lipídeos assim que o inverno se aproxima, e permite que a ave sobreviva naquela estação. Pouco se conhece sobre esses padrões em aves tropicais. O presente trabalho verifica a dinâmica de deposição de gordura de Phacellodomus rufifrons (Wied, 1821, um furnarídeo (Passeriformes, Furnariidae endêmico da região Neotropical, em um ambiente tropical bastante sazonal, o cerrado do sudeste do Brasil. O trabalho de campo foi realizado entre agosto de 2001 a julho de 2002, no Parque Nacional da Serra do Cipó, município de Jaboticatubas, Minas Gerais. Os dados de gordura subcutânea foram classificados visualmente em aves capturadas. Foram estabelecidas quatro categorias para a variação da gordura subcutânea depositada na cavidade da fúrcula. Foi encontrado um aumento significativo nos níveis de gordura ao longo do dia, como para espécies de regiões temperadas, mas não ouve variação ao longo das estações do ano. A massa corporal média não variou ao longo do ano e nem ao longo do dia. Os resultados mostram que variações diárias no cerrado podem atuar como fator de estresse que pode desencadear a adaptações fisiológicas para atravessar a noite. Por outro lado, variações estacionais podem ser balanceadas pelo habito dos indivíduos de P. rufifrons pernoitarem durante todo o ano dentro do ninho, um comportamento relativamente raro entre os Passeriformes.It is known that birds inhabiting temperate regions accumulate fat in order to survive a night of extreme conditions. Levels of fat depots arise as the day goes by, reaching its pick at dusk and decreasing along the night hours when no

  4. Review: Klaus Hurrelmann; Erik Albrecht: Die heimlichen Revolutionäre: Wie die Generation Y unsere Welt verändert und Bernhard Heinzlmaier; Philipp Ikrath: Generation Ego: Die Werte der Jugend im 21. Jahrhundert

    Directory of Open Access Journals (Sweden)

    Jasper, Merle

    2014-12-01

    Full Text Available Kritik an der Jugend wurde schon von Platon in der Politea geübt. Sie hat bis heute Konjunktur. In diesem Diskurs verwundert der Titel von Hurrelmanns und Albrechts Buch Die heimlichen Revolutionäre. Wie die Generation Y unsere Welt verändert. Anhand von aktuellen Studien und Gesprächen mit jungen Menschen versuchen die Autoren ein Porträt der Generation Y zu zeichnen, wobei sie darauf achten, die verschiedenen Lebensbereiche der Jugendlichen in ihrer Analyse abzu - decken. Ein eigener Theorieentwurf oder ein Bezug zu existierenden Theorien findet sich im Buch nicht. Für Hurrelmann und Albrecht kennzeichnet die Generation Y mehr als alles Andere das Hinterfragen „(… bislang scheinbar eherne[r] Grundsätze in Arbeit, Familie, Politik und Freizeit. Sie nutzt spielerisch das Internet, zeigt sich unbekümmert ob der Unsicherheit in der Arbeitswelt und versucht, möglichst gute Leistung zu bringen.“ (14. Eine konträre Meinung über die heutige Jugend findet sich im Buch Generation Ego. Die Werte der Jugend im 21. Jahrhundert von Heinzlmaier und Ikrath. Die Prämisse der Autoren ist, dass das kapitalistische Wirtschaftssystem einen wichtigen Einfluss auf die Jugend habe und diese nur vor dieser Hintergrundfolie adäquat beschrieben werden könne. Nach Ansicht der Autoren kommt es im Allgemeinen zu einer Verlängerung der Jugendphase. Der heutigen Jugend böten sich ständig neue Alternativen. Diese Vielfalt an Optionen führe zu Stress und der Angst, die falsche Wahl zu treffen. Der kapitalistischen Gesellschaft als Ganzes werfen die Verfasser vor, ihr größtes Tabu sei der Pessimismus.

  5. Wie viel Deutschland steckt im Porsche?

    OpenAIRE

    Ferdinand Dudenhöffer

    2005-01-01

    Prof. Dr. Ferdinand Dudenhöffer, Fachhochschule Gelsenkirchen, beschäftigt sich in seinem Kommentar mit der Frage nach der Höhe des Auslandsanteils an den Fertigungskosten bei einigen deutschen Automobilherstellern. Dabei beschäftigt er sich insbesondere mit dem Porsche Cayenne. Er zeigt, dass der deutsche Fertigungsanteil an diesem Auto bei etwa 33% und nicht bei den von Porsche behaupteten 55% liegt. Beim Porsche Cayenne ist der deutsche Wertschöpfungsanteil wesentlich kleiner als bei ander...

  6. Wie weiter mit der Weltsystem-Analyse ?

    DEFF Research Database (Denmark)

    Czeskleba-Dupont, Rolf

    2006-01-01

    Verdenssytem-analysen vender sig imod opfattelsen af ubønhørlige samfundsfremskridt for at være med til at muliggøre reelle fremskidt i overgangen til et nyt verdenssystem. Globaliseringsspørgsmål bliver f.eks. inden for politisk geografi analyseret af fortalere for verdenssystem-analysen idet de...

  7. Van wie zijn zij? De ambtenaren

    NARCIS (Netherlands)

    Peters, Jit

    2011-01-01

    Aan de hand van actuele voorbeelden gaat Jit Peters tijdens zijn afscheidscollege in op de toegenomen macht van ambtenaren. De loyaliteit van de ambtenaar is volgens hem nu te veel gericht op het ondersteunen van de eigen minister. Als tegenwichten van de macht van de ambtenaar staat Peters stil bij

  8. Wie Städte kommunizieren (sollten)

    DEFF Research Database (Denmark)

    Zenker, Sebastian

    2016-01-01

    Im Stadtmarketing versuchen wir die Marke oft durch (teure) Kommunikationsmaßnahmen in die Köpfe unserer Konsumenten zu bringen – dabei geht es auch effizienter.......Im Stadtmarketing versuchen wir die Marke oft durch (teure) Kommunikationsmaßnahmen in die Köpfe unserer Konsumenten zu bringen – dabei geht es auch effizienter....

  9. Nichts ist so stetig wie der Wandel

    DEFF Research Database (Denmark)

    Brem, Alexander; Fuchs, Ch.

    2013-01-01

    management is widely spread and a common theory has not yet been established. Due to the weak focus on SMEs, this paper proposes a model for the transformation of SMEs based on qualitative research in four enterprises. The model is based on an incremental and intuitive change process. It gains theoretical...

  10. Wie viele Tempora hat das Deutsche?

    Directory of Open Access Journals (Sweden)

    Abraham P. ten Cate

    2004-05-01

    Full Text Available This contribution deals with the question of how many tenses exist in the German language. Answers to this question range from a minimum of one up to no less than about thirteen, but the main problem is, what exactly has to be counted. One has to distinguish between tense forms and temporal functions, and standard German has about 6 forms and 6 functions. Almost every form expresses more than one function, so that about 12 pairs of form and function may be found. Such overlap is not necessary, because speakers can avoid using two forms for one function. However, since this situation is not excluded in principle, we have to accept a certain redundancy in the German tense system.

  11. Wie eet de eko-karbonade?

    NARCIS (Netherlands)

    Kruys, van de I.; Meuwissen, M.P.M.; Lans, van der I.

    2001-01-01

    In opdracht van het project Ketenmanagement Biologisch Varkensvlees van Platform Biologica deed WUR onderzoek naar het profiel van de huidige en toekomstige eko-vlees-consument. In supermarkten werd consumenten (onderscheiden in 'eko-consumenten', 'overwegen-eko-consumenten' en 'niet-eko-consumenten

  12. Wie ist Verantwortungsethik möglich?

    OpenAIRE

    Lin, Yuan-Tse

    2010-01-01

    This dissertation consists of two shorter parts (I and II) and a longer main part III. Part I explains why an ethics of responsibility ("Verantwortungsethik") is necessary in a technologically dominated age. In part II the nature and limits of an ethics of responsibility are discussed. Part III begins with a survey of the development of discourse-ethical ("Diskursethik") strategies to justify an ethics of responsibility. Then it argues that a transcendental-pragmatic ("transzendentalpragmatis...

  13. Wie passt der LHC in den Physikunterricht?

    CERN Document Server

    AUTHOR|(CDS)2084439; Woithe, Julia; Brown, Alex; Jende, Konrad

    2017-01-01

    Der LHC bietet sich als aktuelles und prominentes Beispiel der Grundlagenforschung an, im Unterricht behandelt zu werden. Der Artikel gibt einen kurzen Überblick über Komponenten und Funktionsweise des LHC. Zudem wird auf hilfreiche Ressourcen und Anknüpfungspunkte zum Curriculum verwiesen.

  14. Genome-wide identification and comparative expression analysis reveal a rapid expansion and functional divergence of duplicated genes in the WRKY gene family of cabbage, Brassica oleracea var. capitata.

    Science.gov (United States)

    Yao, Qiu-Yang; Xia, En-Hua; Liu, Fei-Hu; Gao, Li-Zhi

    2015-02-15

    WRKY transcription factors (TFs), one of the ten largest TF families in higher plants, play important roles in regulating plant development and resistance. To date, little is known about the WRKY TF family in Brassica oleracea. Recently, the completed genome sequence of cabbage (B. oleracea var. capitata) allows us to systematically analyze WRKY genes in this species. A total of 148 WRKY genes were characterized and classified into seven subgroups that belong to three major groups. Phylogenetic and synteny analyses revealed that the repertoire of cabbage WRKY genes was derived from a common ancestor shared with Arabidopsis thaliana. The B. oleracea WRKY genes were found to be preferentially retained after the whole-genome triplication (WGT) event in its recent ancestor, suggesting that the WGT event had largely contributed to a rapid expansion of the WRKY gene family in B. oleracea. The analysis of RNA-Seq data from various tissues (i.e., roots, stems, leaves, buds, flowers and siliques) revealed that most of the identified WRKY genes were positively expressed in cabbage, and a large portion of them exhibited patterns of differential and tissue-specific expression, demonstrating that these gene members might play essential roles in plant developmental processes. Comparative analysis of the expression level among duplicated genes showed that gene expression divergence was evidently presented among cabbage WRKY paralogs, indicating functional divergence of these duplicated WRKY genes.

  15. THERMAL TREATMENT WITH HOT AIR IN THE CONTROL OF FRUIT FLY IN SAPOTA

    Directory of Open Access Journals (Sweden)

    João Vilian de Moraes Lima Marinus

    2008-08-01

    Full Text Available One of the main postharvest problems of the sapota (Achras sapota L. is caused by the fruit fly (Ceratitis capitata, affecting its quality and commercialization. With this experiment, the objective was to evaluate the effect of hot air as thermal treatment in the control of immature phases of Ceratitis capitata and the fruit quality. The research was carried out at Entomology Laboratory – Centro de Ciências Agrárias, UFPB, Areia-PB. It was made the damaging of fruits with eggs inoculation of Ceratitis capitata and after three days the fruits were treated in hot air at temperatures of 46±1°C and 50±1°C, during 30, 45, 60, 75 and 90 minutes. Hot air was injected in the chamber using an equipment of the Skill mark. It was used a randomized design, in factorial scheme of 2x5+1 (two temperatures, five times of exhibition and one control, with four replications. The treatment with hot air was efficient to control the immature phases of C. capitata during 75 and 90 minutes of exposure and didn't affect the physical and chemical qualities of the sapota fruits.

  16. The biologically equivalent dose BED - Is the approach for calculation of this factor really a reliable basis?; Die biologisch aequivalente Dosis BED - wie solide ist die Berechnung dieses Faktors? Eine Betrachtung der Fehlerbalken der biologisch aequivalenten Dosis

    Energy Technology Data Exchange (ETDEWEB)

    Jensen, J.M. [Kiel Univ. (DE). Klinik fuer Strahlentherapie (Radioonkologie); Zimmermann, J. [Marburg Univ. (DE). Klinik fuer Strahlentherapie (Radioonkologie)

    2000-07-01

    To predict the effect on tumours in radiotherapy, especially relating to irreversible effects, but also to realize the retrospective assessment the so called L-Q-model is relied on at present. Internal specific organ parameters, such as {alpha}, {beta}, {gamma}, T{sub p}, T{sub k}, and {rho}, as well as external parameters, so as D, d, n, V, and V{sub ref}, were used for determination of the biologically equivalent dose BED. While the external parameters are determinable with small deviations, the internal parameters depend on biological varieties and dispersons: In some cases the lowest value is assumed to be {delta}={+-}25%. This margin of error goes on to the biologically equivalent dose by means of the principle of superposition of errors. In some selected cases (lung, kidney, skin, rectum) these margins of error were calculated exemplarily. The input errors especially of the internal parameters cause a mean error {delta} on the biologically equivalent dose and a dispersion of the single fraction dose d dependent on the organ taking into consideration, of approximately 8-30%. Hence it follows only a very critical and cautious application of those L-Q-algorithms in expert proceedings, and in radiotherapy more experienced based decisions are recommended, instead of acting only upon simple two-dimensional mechanistic ideas. (orig.) [German] Um bei der Strahlentherapie von Tumoren die Wirkung, vor allem hinsichtlich irreversibler Effekte, zu prognostizieren, aber auch retrospektive Beurteilungen durchzufuehren, wird z.Z. auf das sog. LQ-Modell vertraut. Interne organspezifische Parameter, {alpha}, {beta}, {gamma}, T{sub p}, T{sub k} und {rho}, und externe Parameter, wie D, d, n, V und V{sub ref}, (Erlaeuterungen im Text) werden zur Bestimmung einer biologisch aequivalenten Dosis BED herangezogen. Waehrend die externen Parameter mit geringem Fehler bestimmbar sind, unterliegen die internen Parameter biologischen Varianzen und Streuungen, in manchen Faellen ist der

  17. Wie nutzen deutsche Tiermedizinerinnen und Tiermediziner soziale Netzwerke? Eine Untersuchung am Beispiel des tiermedizinischen Netzwerks „NOVICE“ [How do German veterinarians use social networks? A study, using the example of the 'NOVICE' veterinary medicine network

    Directory of Open Access Journals (Sweden)

    Schaper, Elisabeth

    2013-02-01

    figures developed very positively during the assessed time period. Until now, the focus of the content of contributions in NOVICE (Network of Veterinary ICT in Education has been on veterinary medicine teaching supported by e-Learning. An increase in the number of members would, however, be beneficial in order to further develop the network so that valuable exchange of information and informal learning can also take place in other specialist areas of veterinary medicine. [german] Zielsetzung: NOVICE (Network Of Veterinary ICt in Education, ist ein soziales professionelles online-Netzwerk für Tiermediziner, Dozierende und Studierende der Tiermedizin sowie für E-Learning-Berater bzw. Didaktiker der veterinärmedizinischen Bildungsstätten. In dieser Arbeit sollte untersucht werden, wie die Akzeptanz bei deutschen Tierärzten, Dozierenden und Studierenden der Veterinärmedizin sowie E-Learning-Beauftragten für ein fachspezifisches Netzwerk ist, welche Anforderungen durch die Nutzer an soziale online-Netzwerke gestellt werden, wie der Umgang mit Web 2.0-Werkzeugen , erfolgt und welches Potential ein berufsspezifisches Netzwerk bieten könnte. Methodik: Die Untersuchung erfolgte mittels Datenanalyse durch die Datenbank der Elgg-Plattform sowie zusätzlich mit google analytics. Jährliche Fokusgruppenbefragungen und Einzelinterviews wurden durchgeführt, um eine Akzeptanzanalyse der Netzwerk-Nutzer vorzunehmen.Ergebnisse: In NOVICE haben sich zwischen dem 01. September 2010 und 21. März 2012 1961 Mitglieder aus 73 Ländern registriert, dabei hat Deutschland mit 565 Nutzern (28,81% den größten Anteil. In diesem Zeitraum kamen auch die meisten Einzelbesuche der Webseite aus Deutschland. Insgesamt sind im Netzwerk 24,83% aller Mitglieder und 19,22% der deutschen Mitglieder aktiv. In Bezug auf das Geschlecht befinden sich sowohl in Hinblick auf alle Mitglieder als auch auf die deutschen Mitglieder deutlich mehr Frauen als Männer in NOVICE. Die am häufigsten verwendeten Web

  18. 基于生态位模型的番荔枝实蝇潜在适生性分布预测%Prediction of the potential distribution of Ceratitis anonae in China based on ecological niche modeling

    Institute of Scientific and Technical Information of China (English)

    李白尼; 马骏; 侯柏华; 张润杰

    2009-01-01

    番荔枝实蝇Ceratitis artortoe(Graham)是一种重要的外来人侵性检疫害虫.在广东口岸,其幼虫连续从入境旅客所携带的水果中被榆出.目前关于番荔枝实蝇潜在适生性分布的研究进行得很少,但对于我国的生物生态安全却有重要意义.本研究中,我们使用3种牛态位模型(ENFA模型,马氏典型性模型和Maxent模型)对番荔枝实蝇在中斟以及全球范尉内的潜在适生性分布区域进行了预测分析.结果显示:Maxent模型拥有最好的预测精确度,马氏典型件模型次之,而ENFA模型的预测精确度最差;Maxent模型和马氏典型性模型的预测精确度无显著性差异;根据Maxent模型的预测结果,番荔枝实蝇在中国的潜在适生区主要是广西、广东、海南以及云南的少部分地区.分析结果显示,番荔枝实蝇从境外传人中国南部地区并最终在上述地区定殖的风险可能性存在,但风险较小.另外,折刀法(Jackknife)分析显示,6种环境因子,例如地面霜冻频率、年平均降雨量、十月降雨量、四月降雨量、年最低温度以及蒸气压,对于番荔枝实蝇在全球和局部地区的分布模式有显著的影响.

  19. Prediction of the potential geographic distribution of Ceratitis rosa Karsch in China%利用CLIMEX预测纳塔尔实蝇在中国的潜在地理分布

    Institute of Scientific and Technical Information of China (English)

    张哲; 李志红; 万方浩; 倪文龙; 曲伟伟

    2011-01-01

    纳塔尔实蝇Ceratitis rosa Karsch属双翅目实蝇科腊实蝇属,为害30余种经济植物,被我国列为进境植物检疫性有害生物.本研究运用CLIMEX 3.0及ArcGIS 9.3对纳塔尔实蝇在我国目前及未来的潜在地理分布进行了预测.结果显示:在目前的气候条件下,纳塔尔实蝇在我国的潜在地理分布区为18.250°N~30.250°N,其中,华南和东南地区为高度潜在地理分布区,包括四川、重庆、云南、福建、广东、广西、海南以及台湾等地.在未来的气候条件下,2020和2050年潜在地理分布区的北界分别移至31.250°N和32.250°N,且中度潜在地理分布区北移明显;2100年潜在地理分布区的北界移至33.750°N,且高度适生区北移明显.因此建议目前应加强纳塔尔实蝇检疫措施,完善监测体系,监测网点主要设在我国的南方地区,尤其应对云南、广西、广东、福建和海南等地进行长期监测;同时,应对湖南、贵州、江西、湖北、江苏、安徽等省进行定期监测,严防该虫入侵.

  20. Fähigkeitsselbstkonzept, Selbstwirksamkeit & Mindset – Wie können Lehrkräfte Erkenntnisse aus der Sozial-Kognitiven-Psychologie nutzen, um die Potenzialentfaltung von Schülerinnen und Schülern zu fördern?

    OpenAIRE

    Breker, Tim

    2016-01-01

    Ausgehend von der Frage, wie Erkenntnisse aus der Sozial-Kognitiven-Psychologie Lehrkräfte dabei unterstützen können, dass Schüler ihr volles Leistungspotenzial abrufen, wird in dieser Forschungsarbeit die Potenzialentfaltungsmatrix als Diagnose- und Förderinstrument für die Schulpraxis entwickelt. Im Theorieteil werden dazu die Theorien zu Fähigkeitsselbstkonzept, Selbstwirksamkeit und Mindset sowie ihre Bedeutung für die Motivation und das Verhalten von Schülern dargestellt. Auf Grundla...

  1. Wirklich wie im wirklichen Leben. Sarah Kember untersucht die (Re-Produktion des kleinen Unterschieds und seiner großen Folgen in Theorie und Praxis von Artificial Life Real as in real life. Sarah Kember examines the (reproduction of ‘the small difference and its big consequences’ in the theory and practice of artificial life.

    Directory of Open Access Journals (Sweden)

    Verena Kuni

    2004-03-01

    Full Text Available Wie greifen diese ‚virtuellen Realitäten‘ in unsere Körper- und Identitätskonzepte, unsere Subjekt- und Geschlechtervorstellungen ein? Sarah Kembers Buch verspricht, erhellende Schneisen durch das Dickicht der definitionsmächtigen Diskurse, Konzepte und Konstruktionen zu schlagen und neue Wege für feministische Interventionen in die Auseinandersetzungen um ‚Artificial Life‘ aufzuzeigen.ow a way through the labyrinth of discourse, concepts and constructions, and to open the door for feminist interpretations in debates about artificial life.

  2. Permanent Genetic Resources added to Molecular Ecology Resources Database 1 December 2009-31 January 2010.

    Science.gov (United States)

    Anderson, Cynthia M; Aparicio, Gallego J; Atangana, Alain R; Beaulieu, Jean; Bruford, M W; Cain, Forrest; Campos, T; Cariani, A; Carvalho, M A; Chen, Nan; Chen, P P; Clamens, A-L; Clark, Ann M; Coeur D'Acier, A; Connolly, Paul; Cordero-Rivera, Adolfo; Coughlan, James P; Cross, Thomas S; David, Bruno; DE Bruyn, Colin; DE Meyer, M; DE Ridder, Chantal; Delatte, H; Dettori, M T; Downer, S J; Dubreuil, Christine; Evans, K J; Fan, Bin; Ferrara, G; Gagné, André; Gaillard, Maria; Gigliarelli, L; Giovinazzi, J; Gomez, D R; Grünwald, N J; Hansson, Bengt; Huotari, T; Jank, L; Jousselin, E; Jungmann, L; Kaczmarek, M E; Khasa, Damase P; Kneebone, Jeff; Korpelainen, H; Kostamo, K; Lanfaloni, L; Lin, Haoran; Liu, Xiaochun; Lucentini, L; Maes, G E; Mahaffee, W F; Meng, Zining; Micali, S; Milano, I; Mok, H F; Morin, L; Neill, T M; Newton, Craig H; Gigi Ostrow, D; Palomba, A; Panara, F; Puletti, M E; Quarta, R; Quilici, S; Ramos, A K B; Rigaud, Thierry; Risterucci, A M; Salomon, Matthew P; Sánchez-Guillén, Rosa A; Sarver, Shane K; Sequeira, A S; Sforça, D A; Simiand, C; Smith, Brian; Sousa, A C B; Souza, A P; Stepien, C C; Stuckert, A J; Sulikowski, James; Tayeh, A; Tinti, F; Tsang, Paul C W; VAN Houdt, J K J; Vendramin, E; Verde, I; Virgilio, M; Wang, Huan L; Wang, L E; Wattier, Rémi A; Wellenreuther, Maren; Xie, Cong X; Zane, L; Zhang, Xiu J; Zhang, Yong; Zhuang, Zhimeng; Zucchi, M I

    2010-05-01

    This article documents the addition of 220 microsatellite marker loci to the Molecular Ecology Resources Database. Loci were developed for the following species: Allanblackia floribunda, Amblyraja radiata, Bactrocera cucurbitae, Brachycaudus helichrysi, Calopogonium mucunoides, Dissodactylus primitivus, Elodea canadensis, Ephydatia fluviatilis, Galapaganus howdenae howdenae, Hoplostethus atlanticus, Ischnura elegans, Larimichthys polyactis, Opheodrys vernalis, Pelteobagrus fulvidraco, Phragmidium violaceum, Pistacia vera, and Thunnus thynnus. These loci were cross-tested on the following species: Allanblackia gabonensis, Allanblackia stanerana, Neoceratitis cyanescens, Dacus ciliatus, Dacus demmerezi, Bactrocera zonata, Ceratitis capitata, Ceratitis rosa, Ceratits catoirii, Dacus punctatifrons, Ephydatia mülleri, Spongilla lacustris, Geodia cydonium, Axinella sp., Ischnura graellsii, Ischnura ramburii, Ischnura pumilio, Pistacia integerrima and Pistacia terebinthus.

  3. Wie hoch liegen die Personalkosten für die Durchführung einer OSCE? Eine Kostenaufstellung nach betriebswirtschaftlichen Gesichtspunkten [How high are the personnel costs for OSCE? A financial report on management aspects

    Directory of Open Access Journals (Sweden)

    Rau, Thea

    2011-02-01

    erprobt. Für die Umsetzung einer OSCE ist ein hoher Personaleinsatz erforderlich. Insbesondere für Fächer mit geringen Ressourcen ist daher eine effiziente Kostenplanung erforderlich. In der Klinik für Neurologie der Medizinischen Fakultät der Universität Ulm wurde die OSCE im Wintersemester 09/10 als Pilotprojekt eingeführt und retrospektiv eine Kostenaufstellung der Personalaufwendungen vorgenommen. Sie soll beispielhaft einen Einblick in die erforderlichen Mittel für eine OSCE mit Simulationspatienten geben. Methode: In die Berechnung des finanziellen Aufwands für die OSCE gingen ein: Anstellungsstatus des Personals mit Einsatzbereich für die OSCE, inhaltliche und zeitliche Dimension des Einsatzes. Nach der Prüfung wurde die Akzeptanz des Prüfungsformates durch ein Fokusgruppeninterview mit den Lehrenden und Studierenden untersucht.Ergebnis: Die Gesamtaufwendungen für den Personaleinsatz für die OSCE betrugen 12.468,- €. Der von der Klinik aufzubringende Anteil errechnete sich mit 9.576,- €. Studiengebühren wurden in Höhe von 2.892,-€ verwendet. Bei Umrechnung der Gesamtaufwendungen auf die Anzahl der Prüflinge errechnete sich ein Betrag in Höhe von 86,- € pro Prüfling. Sowohl die Studierenden als auch die Lehrenden bestätigten die Validität der Prüfung und sahen in der neuen Prüfung den Mehrwert in den Lerneffekten. Schlussfolgerung: Die hohe Akzeptanz der OSCE in der Neurologie bei Studierenden und Lehrenden spricht für eine Beibehaltung des Prüfungsformates. Vor dem Hintergrund des hohen finanziellen und logistischen Aufwands einer OSCE sollte jedoch im Einzelfall geprüft werden, wie langfristig eine effiziente Prüfungsdurchführung möglich ist.

  4. 两类甘蓝雄性不育系种子产量构成因素分析%Analysis of Seed Yield Components between Two Cabbage ( Brassica oleracea var.capitata L.) Male Sterile Lines

    Institute of Scientific and Technical Information of China (English)

    王庆彪; 方智远; 张扬勇; 刘玉梅; 杨丽梅; 庄木; 陈琛

    2011-01-01

    The differences of seed yield and component factors among dominant genie male sterile line DGMS02-12, cytoplasmic male sterile line CMSR302-12 and inbred line of cabbage ( Brassica oleracea var. Capitata L. ) 02-12 were studied. The results showed that the differences of seed yield per plant and plot yield between DGMS02-12 and CMSR302-12 were significant. The whole plant valid siliqua and seeds per siliqua were the main factors for the differences of seed yield between these two male sterile lines of cabbage. The seed yield at the 1st branch account for more than 80% of per plant seed yield, and there were significantly positive correlations and high path coefficient between seed yield and seeds per siliqua and the valid siliqua at the 1st branch.%以结球甘蓝自交系02-12及其回交转育多代育成的显性核基因雄性不育系(DGMS)和细胞质雄性不育系(CMS)为试材,对其制种产量及种子产量构成因素进行分析.结果表明:显性核基因雄性不育系材料DGMS02-12与细胞质雄性不育系材料CMSR302-12在单株产量和小区产量方面存在显著差异,并且全株有效荚数和每荚种子粒数是两种类型甘蓝雄性不育系间制种产量存在差异的主要构成因素.其中,一级分枝种子产量占单株产量的80%以上,一级分枝有效荚数、每荚种子粒数与制种产量有显著的相关性和较高的通径系数.

  5. ANÁLISE FAUNÍSTICA DE ESPÉCIES DE MOSCA-DAS-FRUTAS (DIP., TEPHRITIDAE EM MINAS GERAIS

    Directory of Open Access Journals (Sweden)

    N.A. CANAL

    1998-01-01

    Full Text Available Em seis locais de quatro municípios (Janaúba, Jaíba, Nova Porteirinha e Itacarambí do norte do Estado de Minas Gerais, foram coletados 29.454 espécimes de mosca-das-frutas, pertencentes a Ceratitis capitata e a 20 espécies de Anastrepha. O levantamento foi feito entre janeiro de 94 e dezembro de 96, utilizando armadilhas plásticas tipo McPhail. Ceratitis capitata foi a espécie predominante em áreas urbanas. As espécies de Anastrepha predominaram em áreas rurais. A. obliqua, A. zenildae e Anastrepha n. sp.3 foram as espécies predominantes do gênero, entretanto, essa predominância variou de local para local em função da disponibilidade de hospedeiros. As comunidades apresentaram índices de diversidade baixos e quocientes de similaridade entre 73 e 100%.A total of 29,454 specimens of fruit fly were trapped in six sites of four counties (Janaúba, Jaíba, Nova Porteirinha and Itacarambí of the north of the State of Minas Gerais, Brazil. The specimens were collected using McPhail plastic traps from January 1994 to December 1996. The trapped fruit flies belonged to Ceratitis capitata and to 20 species of Anastrepha. Ceratitis capitata was the predominant species in the urban areas and Anastrepha species were predominant in the field areas. A. obliqua, A. zenildae and Anastrepha n. sp.3 were the predominant species of the genera, whereas the predominant species differed among localities, according to host availability. The diversity indexes were low and the coefficient of similarity varied from 73 to 100%.

  6. Dicty_cDB: Contig-U16208-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 67428 ) FLD1_68_H08.g1_A029 Root flooded Pinus taeda cDNA... 38 0.76 2 ( CN753675 ) ID0AAA10DD04RM1 ApMS Acy...2 ( FG075806 ) UI-FF-IF0-abm-h-15-0-UI.r1 Ceratitis capitata emb... 34 0.77 2 ( CO159864 ) FLD1_16_B09.g1_A029 Root flood

  7. Biology of Fopius arisanus (Hymenoptera: Braconidae) in Two Species of Fruit Flies

    Science.gov (United States)

    Groth, M. Z.; Loeck, A. E.; Nörnberg, S. D.; Bernardi, D.; Nava, D. E.

    2016-01-01

    Fopius arisanus (Sonan, 1932) (Hymenoptera: Braconidae) is an egg–larval parasitoid used in control programs of Bactrocera dorsalis (Hendel) and Ceratitis capitata (Wiedemann). In Brazil, C. capitata and Anastrepha fraterculus (Wiedemann) are considered the main tephritid pests of exotic and indigenous fruits. The objective of this study was to study the biology of F. arisanus in C. capitata and A. fraterculus. Eggs of the two fruit fly species were used to determine the parasitism rate, number of offspring, emergence rate, sex ratio, adult weight and longevity of male and female F. arisanus. These biological parameters were used to develop a fertility life table. We observed higher parasitism and emergence rates of adults, a shorter duration of the egg–adult period and a sex ratio biased to females when F. arisanus was reared in eggs of C. capitata than in those of A. fraterculus. However, adults of F. arisanus from eggs of A. fraterculus were heavier and had greater longevity than those obtained from C. capitata eggs. The fertility life table showed better biological and reproductive performance for F. arisanus reared in eggs of C. capitata, although eggs of A. fraterculus also provided positive values for population increase. PMID:27638954

  8. Effect of Magnetic Field on Brassica oleracea L. var. capitata L. Seed Germination and Studies on Its Mechanism%磁场对结球甘蓝种子萌发的影响及机理研究

    Institute of Scientific and Technical Information of China (English)

    崔路路; 于海霞; 李景富; 姜景彬; 许向阳

    2014-01-01

    以结球甘蓝种子为试材,采用正交试验和均匀试验方法,研究了磁场强度、作用时间及处理前种子浸泡时间对各发芽指标的影响以及各影响因素的主次顺序,并对磁场参数中磁场强度和作用时间进行大范围筛选,更进一步从可溶性蛋白含量、丙二醛含量、抗氧化酶活性等方面探讨磁场处理提高种子活力的作用机理。结果表明:适宜的磁场处理可以促进种子萌发,提高种子活力,以20℃蒸馏水浸泡8 h 后1000~3500 GS 磁场处理1~6 min 为宜,但长时间、大强度磁场处理反而抑制种子萌发;磁场强度为种子萌发的主要影响因子,作用时间和浸泡时间的主次顺序因发芽指标的不同而不同;与对照相比,磁场处理的种子萌发期间可溶性蛋白含量降低或升高的速率、MDA 含量下降的幅度和速率、3种抗氧化酶活性上升的速率均加快,从而提高种子活力。%Taking Brassica oleracea L. var. capitata L. seed as experimental material,the effect of magnetic field intensity,and magnetic acting time and seed soaking time before treatment on seed germination index and primary and secondary relations between all factors were studied by orthogonal test and uniform test method.Then the large scale screening of magnetic field intensity and magnetic acting time were carried out to further investigate the mechanism of using magnetic field treatment to improve seed vigor from the view points of soluble protein content,malondialdehyde content,and antioxidant enzyme activity.The results indicated that suitable magnetic field treatment could promote seed germination and improve seed vigor.Magnetic field intensity was the main factor affecting seed germination.The primary and secondary order of action time and soaking time were varied with different germination indexes. Seed germination was inhibited by too high magnetic field intensity for a long time. Soaking seed in 20

  9. Short circuit - How our power supply became more expensive and got worse. A critical balance after eight years ''deregulation'' of the German Energy Economy; Kurzschluss - Wie unsere Stromversorgung teurer und schlechter wurde. Eine kritische Bilanz nach acht Jahren ''Liberalisierung'' der deutschen Energiewirtschaft

    Energy Technology Data Exchange (ETDEWEB)

    Leuschner, U.

    2007-07-01

    verursachte. Wie Leuschner feststellt, waere der Schaltungsfehler der EON-Netzleitstelle Lehrte vor ein paar Jahren noch vom System toleriert worden. Er habe nur deshalb so gravierende Folgen haben koennen, weil das UCTE-Netz inzwischen durch den Stromhandel und andere Folgen der Deregulierung bis an die Grenzen seiner Kapazitaet belastet sei. Im dritten Teil (''Netznutzung und Netzentgelte'') beleuchtet er in einem zusaetzlichen Kapitel die Bemuehungen der EU-Kommission, doch noch die Rahmenbedingungen fuer echten Wettbewerb herzustellen. Die neueste Forderung der Kommission nach eigentumsmaessiger Entflechtung der Netze deutet er als ''Flucht nach vorn'' angesichts der Tatsache, dass in saemtlichen EU-Laendern seit der Liberalisierung die Strompreise gestiegen sind und das zum Teil sogar ueber die Haelfte (mit der einzigen Ausnahme Englands, und hier auch nur der Haushaltsstrompreise). Kraeftig erweitert hat der Verfasser auch den vierten Teil zum Thema ''Management und Politik'': Zum einen findet man hier nun beschrieben, wie Ex-Bundeskanzler Gerhard Schroeder einen Spitzenjob beim russischen Energiekonzern Gazprom erhielt, dem er bereits in seiner Amtszeit als Bundeskanzler politisch behilflich war, und wie sein ehemaliger Wirtschaftsminister Wolfgang Clement in den Aufsichtsrat von RWE Power einziehen durfte. Zum anderen wird ein kritischer Blick auf die Werbekampagnen geworfen, mit denen die Energiekonzerne versuchen, ihr Erscheinungsbild in der Oeffentlichkeit aufzuhellen und den Energieverbrauchern ein X fuer ein U vorzumachen. (orig.)

  10. Benefits and risks of benzodiazepines and Z-drugs: comparison of perceptions of GPs and community pharmacists in Germany [Wie schätzen deutsche Hausärzte und Apotheker Nutzen und Schaden von Benzodiazepinen und Z-Drugs ein?

    Directory of Open Access Journals (Sweden)

    Hoffmann, Falk

    2013-07-01

    Full Text Available [english] Objective: Newer non-benzodiazepines zolpidem and zopiclone are often prescribed instead of benzodiazepine hypnotics, although there is no evidence of differences in effectiveness and safety. Aim was to compare perceptions on benefits and harms of benzodiazepines and Z-drugs between general practitioners (GPs and community pharmacists (CPs.Methods: A questionnaire was mailed to a random sample of 1,350 GPs and 600 CPs in 2012. They were asked to rate perceptions on a five-point Likert scale used for both benzodiazepines and Z-drugs. Wilcoxon signed rank test was performed for the comparison of perceptions between GPs and CPs. Due to multiple testing, only p-values ≤0.01 were considered statistically significant.Results: 458 GPs and 202 CPs returned questionnaires (response 33.9% and 33.7%. Mean age of GPs was 53.3 years (40.6% female and 48.8 years for CPs (59.2% females. Perceptions on benefits of benzodiazepines (and Z-drugs between GPs and CPs were not different for 3 (and 2 of 5 items. Concerning side effects of benzodiazepines, there were no statistically significant differences for 3 of 5 comparisons. CPs perceived that 4 of 5 studied side effects of Z-drugs occur significantly more often than GPs (p=0.003 or less. For instance, whereas 45.2% of CPs answered that withdrawal effects on stopping happen often or very often/always on Z-drugs, these were only 28.3% of the GPs. Conclusions: Although it is difficult to draw unambiguous conclusions from these findings, pharmacists might have a somewhat more critical view on Z-drugs, especially concerning side effects.[german] Hintergrund und Fragestellung: Die neueren Benzodiazepinrezeptor-Agonisten Zolpidem und Zopiclon (“Z-Drugs” werden in letzter Zeit häufiger als Hypnotika vom Benzodiazepintyp verschrieben, obwohl keine Belege für Unterschiede bezüglich des Nutzens und Schaden existieren. Ziel dieser Studie war es zu vergleichen, wie Hausärzte und Apotheker erwünschte und

  11. Wie häufig und belastend sind negative Erfahrungen im Medizinstudium? – Ergebnisse einer Online-Befragung von Medizinstudierenden [Frequency and perceived severity of negative experiences during medical education in Germany – Results of an online-survery of medical students

    Directory of Open Access Journals (Sweden)

    Gágyor, Ildikó

    2012-08-01

    Belästigung, Benachteiligung aufgrund von Herkunft, Nationalität oder Hautfarbe, und anderen Formen negativer Erfahrungen befragt. Ergebnisse: Von 391 Studierenden berichteten 56% von mangelnder Wertschätzung, 51% davon, Konkurrenz erlebt zu haben, und 34% von verbal unangemessener Behandlung. Während 59% sich durch verbal unangemessene Behandlung belastet fühlten, lag der Anteil der Belasteten bei mangelnder Wertschätzung bzw. Konkurrenz bei 46% bzw. 32%. Diese Rate war in anderen Fällen, wie bei der Erfahrung „Übergangen oder nicht beachtet zu werden“ deutlich niedriger (21%. Weibliche Studierende fühlten sich insgesamt häufiger belastet als männliche. Schlussfolgerung: Weniger spektakuläre Formen wie mangelnde Wertschätzung oder verbal unangemessene Behandlung sind nicht nur häufig, sondern werden auch als belastend empfunden. Lehrende in der medizinischen Ausbildung sollten die Gefahr, Studierende gerade auch auf diese Weise zu verletzen, kritisch reflektieren.

  12. MOSCAS-DAS-FRUTAS (DIPTERA: TEPHRITIDAE EM POMARES DA ÁREA URBANA NO NORTE DE MINAS GERAIS

    Directory of Open Access Journals (Sweden)

    CLARICE DINIZ ALVARENGA

    2010-01-01

    Full Text Available The aim this work was know the species of fruit fly and host plants in orchards in the urban area in the north of Minas Gerais. Were selected 10 orchards with wide variety of fruit species, which were distributed in equidistant way in the urban area of Janaúba, MG. Weekly, were collected systematically fruit flies through trap type McPhail and ripe fruit and in ripening one, on those orchards. Were collected 7.016 tephritid obtained from trap (5.226 and fruit (1.790, from which 1.044 belonged to genus Anastrepha and 5.972 were Ceratitis capitata. The specimens number of C. capitata (85.1% was around six times superior to Anastrepha spp. (14.9%, demonstrating the preference of this species for urban orchards. Eight species of Anastrepha occur in urban orchards of Janaúba, MG. Ceratitis capitata was found infesting 10 species of host fruits, being the main S. purpurea and guava. In fruits were collected three species of Anastrepha (A. obliqua, A. sororcula and A. zenildae which were associated with five species of fruit (Malpighia glabra L, Psidium guayava L, S. dulcis, S. purpurea and S. tuberosa. The predominant species of Anastrepha was A. obliqua, and S. tuberosa and S. purpurea being the main hosts of this species in the urban area of Janaúba, MG.

  13. Wie messen wir unseren Open-Access-Anteil?

    OpenAIRE

    Tullney, Marco

    2016-01-01

    Open Access ist auf dem Siegeszug. Die „Open-Access-Transformation“ hat sich als neues Schlagwort etabliert, und Verlage konzentrieren sich mehrheitlich darauf, weitere Einnahmen durch Open Access zu generieren, statt die Entwicklung zu bekämpfen. Die von wissenschaftlichen Einrichtungen getragenen Kosten für Open-Access-Artikel steigen (ganz zu schweigen vom enormen Arbeitsaufwand, den manche der zugrundeliegenden Modelle mit sich bringen). Open Access ist ein Thema, das prominent in Auflage...

  14. Wie sind Zeit, Raum und Zustand sprachlich verbunden?

    DEFF Research Database (Denmark)

    Colliander, Peter

    morphosyntaktischen Bereich nachweisbar, und zwar bei der Kasusrektion gewisser Präpositionen. So regiert in nicht nur in lokalen Zusammenhängen bei Zielgerichtetheit den Akkusativ (Jd. geht ins Kino) und bei Nichtzielgerichtetheit den Dativ (Jd. war im Kino), sondern genauso in temporalen Zusammenhangen (Jd. wünscht...

  15. Wie vergleichbar sind Ergebnisse von Rechtschreib-und Lesetests?

    OpenAIRE

    Steinhauser, Susanne

    2006-01-01

    Eine umschriebene Lese-Rechtschreibstörung (ICD-10: F81) ist durch eine Diskrepanz zwischen den allgemeinen kognitiven Fähigkeiten eines Kindes und dessen Lese-Rechtschreibleistungen gekennzeichnet. Für die Diagnostik steht eine Vielzahl von Lese- und Rechtschreibtests zur Verfügung. In der Studie wurde untersucht, ob die Diagnosestellung von der Wahl des Testverfahrens abhängt. Es wurden Kinder mit mehreren Rechtschreib- und Lesetests untersucht. Die allgemeinen kognitiven Fähigkeiten wur...

  16. 'Je moeder leert je wie je vijanden zijn'

    NARCIS (Netherlands)

    Makkink, C.; Parmentier, H.K.

    2014-01-01

    Via de voeding van ouderdieren kan de werking van het immuunsysteem van de nakomelingen worden beïnvloed. Het is een uitdaging om interventies te vinden die het immuunsysteem van landbouwhuisdieren optimaliseren, zo luidde de conclusie tijdens de Feed4Foodure-themamiddag.

  17. Wie viel Kapazität hat ein Mobilfunknetz?

    Science.gov (United States)

    Eisenblätter, Andreas; Geerdes, Hans-Florian

    Wer hat das noch nicht erlebt? Der Akku ist aufgeladen und reichlich Guthaben vorhanden, doch das Telefonieren mit dem Handy klappt trotzdem nicht. Meist liegt das daran, dass man sich in einem Funkloch befindet. Aber manchmal ist auch das Netz überlastet. Anders gesagt: das Funknetz des Mobilfunkanbieters, das viele Antennen auf Hausdächern oder Masten umfasst, bietet entweder nicht genügend Abdeckung oder nicht genügend Kapazität. Genau das will der Anbieter natürlich vermeiden. Mathematik hilft, diese Herausforderungen mit einer guten Planung des Mobilfunknetzes zu bewältigen.

  18. Placentation in the rock cavy, Kerodon rupestris (Wied)

    DEFF Research Database (Denmark)

    Oliveira, M.F.; Carter, Anthony M.; Bonatelli, M.;

    2006-01-01

    Rock cavies are rodents found in the semi-arid caatinga of Brazil. We studied the structure of the rock cavy placenta by light and transmission electron microscopy. The exchange area of the labyrinth was organized in lobes separated by interlobular areas. The interhaemal barrier was syncytial...... uptake from the uterine lumen. Tight junctions between these cells provided a barrier to diffusion by the intercellular route. The reproductive biology of the rock cavy differs from other members of the family, including the guinea pig, but the architecture of the placenta remains remarkably constant....

  19. Wie is die sondaar? Die beskouing van Karl Barth

    Directory of Open Access Journals (Sweden)

    F. J. van Zyl

    1994-01-01

    Full Text Available Who is the sinner? The view of Karl Barth The previous article dealt with Barth’s view of the knowledge and real essence of sin, while this one focus on his view of the human being as sinner. In accordance with his christological approach to all theological matters, Barth presents us with a description of the image and character of the sinner as mirrored by the obedient suffering and death of Jesus Christ on the cross in the place of all sinners of all times - past, present and future. The price that God paid in surrendering his only Son to such suffering, indicates the enormous guilt and baseness of every sinner. Every human being is utterly insolvent and can only be delivered from sin through God’s graceful remission of siru

  20. Wo und wie wird man Weiterbildungsberater/-in?

    OpenAIRE

    Feider, Cornelia

    2007-01-01

    Schwerpunkt dieses Beitrages ist die Darstellung der Ergebnisse einer Internetrecherche im Oktober 2006 zu Aus- und Fortbildungsmöglichkeiten zum/-zur Weiterbildungsberater/-in. Es wird festgestellt, dass es bereits ein breites Angebot gibt. Aus diesem können potenzielle und in der Praxis tätige Weiterbildungsberaterinnen und –berater je nach Vorkenntnissen, finanziellen und zeitlichen Möglichkeiten auswählen. Angesichts der Vielzahl an Optionen zur Fortbildung wird aber auch diskutiert, welc...

  1. Ein altes Problem: Partikeln in der Grammatik - ja, aber wie?

    Directory of Open Access Journals (Sweden)

    Kärnä, Aino

    2005-01-01

    Full Text Available This article deals with the concept 'particle', specifically with the German notion of 'Abtönungspartikel'. These pragmatic or modal particles were first discussed in contemporary German grammars by Harald Weydt. Although the concept itself is clear, it is difficult to include such a category in existing grammars, as is shown by the treatment of such inflexible words in different editions of the standard German grammar, Der Duden. It seems difficult to formulate a description of the word class 'particle' in such a way that it includes pragmatic particles as well as other kinds of particles, such as focus particles. This problem is not new, and I also discuss parallels from the history of linguistics.

  2. Therapie der Multiplen Sklerose: Wer? Was? Wann? Wie lange?

    Directory of Open Access Journals (Sweden)

    Vass K

    2001-01-01

    Full Text Available Die Einführung moderner immunmodulierender Therapien hat neue Perspektiven in der Behandlung der Multiplen Sklerose eröffnet. Erstmals kann zumindest bei einem Teil der betroffenen Patienten die Zahl der Schübe verringert und auch die Progredienz der Behinderung signifikant verlangsamt werden. Vor allem bei Patienten mit schlechterer prognostischer Ausgangslage sollte früh mit einer immunmodulierenden Therapie begonnen werden. Bei wenig Unterschieden im Wirkprofil und ähnlichen Nebenwirkungen ist allerdings die Wahl des Präparats nicht einfach, und es sollte auch die konkrete Lebenssituation des Betroffenen in der Entscheidung berücksichtigt werden.

  3. Wissensmanagementsysteme in Schulen oder: wie bringe ich Ordnung ins Chaos?

    Directory of Open Access Journals (Sweden)

    Andreas Breiter

    2002-11-01

    Full Text Available Die zunehmende Ausstattung von Schulen mit digitalen Medien wird zu einer Herausforderung für die Organisationsentwicklung. Digitale Materialien der Lehrkräfte sowie Schülerarbeiten in digitalisierter Form wachsen tagtäglich an. Bisher fehlen geeignete organisatorische und technische Möglichkeiten zur Archivierung, Verteilung und Nutzung. In Unternehmen hat sich mit dem Begriff Wissensmanagement ein organsatorisches Konzept etabliert, das die Nutzung der Ressource Wissen optimieren will. Um diese Prozesse zu unterstützen, wurden und werden informations- und kommunikationstechnische Werkzeuge entwickelt, deren Übertragung auf den Schulbereich aufgrund dessen organisatorischer Komplexität aber scheitern muss. In diesem Beitrag werden diese Besonderheiten herausgestellt und die Erfahrungen mit der Entwicklung eines webbasierten Wissensmanagementsystems in Zusammenarbeit mit Schulen skizziert.

  4. Wie was Andrew Murray (1828-1917 in werklikheid?

    Directory of Open Access Journals (Sweden)

    T.G. Mahne

    1999-08-01

    Full Text Available Murray (1828-1917 was an emissary of God. In the Dutch Reformed Church in South Africa, where he served as a full time minister for fifty eight years, he was elected Moderator six times. His influence, however, was not limited to the Dutch Reformed Church. Of the two hundred and fifty books (more than 20 000 pages he wrote, some were translated into more than twenty languages. In spite of his intention not to write theological works, Murray was granted a doctorate degree in Theology by the University of Aberdeen in 1898. He was a man of prayer who published approximately thirty books about prayer. Murray, a mystic and peifectionist, was reared in an extremely legalistic home. As a student he joined the Secor Dabar association which was an offspring of the legalistic Reveil movement in the first half of the nineteenth century. At the age of roundabout 65, Murray was impressed by the writings of William Law (1686-1761, which fitted his mindset like a glove. But who was Andrew Murray actually? Other similar questions concerning his influence in the Dutch Reformed Church are equally important. First and foremost however: Who was this man? Was he possibly a "tossed salad" theologian? Still today we find traces of Murrayism in the Dutch Reformed Church. Fortunately his full-time service of fifty eight years has left behind a positive heritage of Scottish Calvinism.

  5. Wie ich mich mit dem äthiopischen Regime anlegte

    DEFF Research Database (Denmark)

    Hagmann, Tobias

    2016-01-01

    Als Forscher und Kritiker der äthiopischen Politik habe ich viel über das Verhältnis gelernt, in dem Wahrheit und Macht, aber auch Moral und persönliche Interessen zueinander stehen. In den Niederungen der Realpolitik wird mit harten Bandagen um die Deutungshoheit gekämpft wird. Auch Wissenschafter...

  6. Ganglion block. When and how?; Ganglienblockade. Wann und wie?

    Energy Technology Data Exchange (ETDEWEB)

    Bale, R. [Medizinische Universitaet Innsbruck, Sektion fuer Mikroinvasive Therapie Universitaetsklinik fuer Radiologie, Innsbruck (Austria)

    2015-10-15

    Increasing understanding of the anatomy and physiology of neural structures has led to the development of surgical and percutaneous neurodestructive methods in order to target and destroy various components of afferent nociceptive pathways. The dorsal root ganglia and in particular the ganglia of the autonomous nervous system are targets for radiological interventions. The autonomous nervous system is responsible for the regulation of organ functions, sweating, visceral and blood vessel-associated pain. Ganglia of the sympathetic chain and non-myelinized autonomous nerves can be irreversibly destroyed by chemical and thermal ablation. Computed tomography (CT)-guided sympathetic nerve blocks are well established interventional radiological procedures which lead to vasodilatation, reduction of sweating and reduction of pain associated with the autonomous nervous system. Sympathetic blocks are applied for the treatment of various vascular diseases including critical limb ischemia. Other indications for thoracic and lumbar sympathectomy include complex regional pain syndrome (CRPS), chronic tumor associated pain and hyperhidrosis. Neurolysis of the celiac plexus is an effective palliative pain treatment particularly in patients suffering from pancreatic cancer. Percutaneous dorsal root ganglion rhizotomy can be performed in selected patients with radicular pain that is resistant to conventional pharmacological and interventional treatment. (orig.) [German] Anatomische und physiologische Kenntnisse ueber die Funktion von Schmerzbahnen fuehrten zur Entwicklung chirurgischer und perkutaner destruktiver Verfahren, um einzelne Komponenten afferenter Schmerzbahnen anzusteuern bzw. auszuschalten. Neben anderen nervalen Strukturen gelten Hinterstrangganglien und insbesondere die Ganglien des autonomen Nervensystems als Ziele fuer radiologische Interventionen. Das vegetative Nervensystem ist fuer die Organfunktion durch Regulation des Gefaesstonus und fuer die Leitung viszeraler und gefaessassoziierter Schmerzen verantwortlich. Durch die CT-gezielte Sympathikolyse mit Alkohol oder durch Thermoablation mit Radiofrequenz werden Ganglien des sympathischen Grenzstrangs bzw. nichtmyelinisierte Nervenbahnen irreversibel zerstoert. Die Blockade des Sympathikus ist ein anerkanntes interventionell-radiologisches Verfahren und fuehrt zu einer Aufhebung der vasokonstriktorischen Wirkung, einer verminderten Schweisssekretion sowie einer Reduktion von Schmerzen, die mit dem vegetativen Nervensystem assoziiert sind. Die Sympathikolyse eignet sich zur Behandlung verschiedener vaskulaerer Erkrankungen, insbesondere der kritischen Extremitaetenischaemie. Zusaetzlich kommt die thorakale und lumbale Sympathikusblockade beim komplexen regionalen Schmerzsyndrom (CRPS), bei tumorbedingten chronischen Schmerzen und bei der Hyperhidrose zum Einsatz. Die Plexus-coeliacus-Blockade ist eine effektive palliative Schmerztherapie und wird insbesondere fuer Patienten mit Pankreaskarzinom eingesetzt. Die perkutane Rhizotomie des Hinterwurzelganglions kann bei ansonsten austherapierten Patienten mit radikulaeren Schmerzen eingesetzt werden. (orig.)

  7. Ueber die pygidialdruese von enhydrus sulcatus (Wied., 1821 (Coleoptera, Gyrinidae

    Directory of Open Access Journals (Sweden)

    Rudolf Barth

    1960-11-01

    Full Text Available As glândulas pigidiais, pares, de enhydrus sulcatus abrem-se, em cada lado, na região pleural do 8º segmento abdominal. A glândula possui um ducto excretor. Sua continuação apical forma um volumoso reservatório dilatável, revestido por um retículo muscular que espreme a secreção. Entre estas duas partes, encontra-se uma válvula, para regular a passagem das secreções, caraterisada por uma estrutura cuticular especial. Na região inicial do reservatório estende-se uma placa glandular. Antes da válvula nasce um tubo glandular composto de um canal central e divertículos laterais. As células da placa glandular produzem uma substância aquosa, possuindo sòmente poucos componentes orgãnicos e que consideramos como sendo o veículo das secreções oleosas do tubo glandular. As células glandulares possuem um aparêlho excretor intra-celular, denominado, por outros autores, como "Binnenblase" (vesícula interna, enquanto que nós o consideramos como sendo um verdadeiro rabdório. O fino tubo cuticular, que penetra neste complexo rabdorial, formando a parte inicial do tubo excretor, representa o verdadeiro pólo apical da célula glandular.

  8. Benzodiazepine absetzen im Alter. Wann und, wenn ja, wie?

    DEFF Research Database (Denmark)

    Wolter, Dirk

    2017-01-01

    Although viewed critically in geriatrics, benzodiazepine use is still common among old people. Before reducing the dosage the following questions must be considered: 1. Are there indications for benzodiazepine treatment and will discontinuation cause relevant rebound symptoms of the initial...... disorder treated? 2. To what extent do the patient and other key persons consider discontinuation to be reasonable and will they support discontinuation? 3. Is the target complete withdrawal, a dose reduction or shift to another benzodiazepine drug which is more suitable in old age for pharmacokinetic...... reasons? This article provides assistance in answering these questions and some guidelines for the practical management of discontinuation. It is mandatory 1) to periodically address the problem of long-term benzodiazepine use when counseling the patient and key persons and 2) to be aware that several...

  9. Ceratólise auto-imune em paciente com vasculite leucocitoclástica: apresentação atípica de erythema elevatum diutinum com padrão granulomatoso Autoimmune keratolysis in a patient with leucocytoclastic vasculitis: unusual erythema elevatum diutinum with granulomatous pattern

    Directory of Open Access Journals (Sweden)

    Fábio Henrique Cacho Casanova

    2000-06-01

    Full Text Available Contexto: A vasculite leucocitoclástica é uma doença que acomete pequenos vasos mais comumente associada a distúrbios do tecido conectivo, caracterizada clinicamente pela presença de lesões purpúricas palpáveis. Manifestações oftalmológicas são raras. Objetivo: Descrever quadro clínico de paciente portador de vasculite leucocitoclástica como manifestação incomum de padrão granulomatoso do erythema elevatum diutinum (EED. Relato de Caso: Paciente de 64 anos, masculino, apresentando baixa de acuidade visual e aparecimento de lesões nodulares em mãos. Ao exame oftalmológico, evidenciou-se afinamento corneano superior bilateral com perfuração corneana do olho esquerdo e espessamento da conjuntiva limbar em ambos os olhos, associada a intensa atividade inflamatória. Exame anatomopatológico da conjuntiva revelou vasculite granulomatosa com intenso exsudato neutrofílico, células gigantes multinucleadas, além de proliferação fibroblástica. Exame anatomopatológico de biopsia da pele evidenciou alterações semelhantes compatíveis com EED, porém não foi visto célula gigante sugerindo lesão granulomatosa. Conclusão: Ceratólise auto-imune secundária a vasculite leucocitoclástica cutânea (EED não havia sido descrito na literatura, tampouco encontramos referências sobre a reação granulomatosa (encontrada na conjuntiva em EED.Purpose: Leucocytoclastic vasculitis is an immune complex-mediated, small vessel disease, that is characterized clinically by the presence of palpable purpuric lesions, most often in association with rheumatic diseases. Ocular manifestations are rare. We describe a patient with an unusual granulomatous pattern of EED associated with autoimmune keratolysis. Case Report: A 64-year-old man with decreased visual acuity and nodular lesions in both hands. Ocular exami-nation presented bilateral superior corneal melting with perfuration in left eye and conjunctival thickness in both eyes, in

  10. 构建结球甘蓝K IN基因在叶绿体基因组定点表达的载体%Construction of Chloroplast Site-specific Integration Expression Vector Harboring KIN Gene of Cabbage (Brassica oleracea L. var. capitata L.)

    Institute of Scientific and Technical Information of China (English)

    陶鹏; 黄小云; 李必元; 王五宏; 岳智臣; 雷娟利; 钟新民

    2015-01-01

    获得叶绿体基因组定点整合表达载体是开展结球甘蓝叶绿体基因组的遗传转化研究的第一步。本研究克隆了CMS结球甘蓝的抗冻蛋白K IN基因,发现该基因定位于结球甘蓝的2号染色体上。通过构建中间载体pKA和pAI,将K IN基因的编码区构建到了CMS结球甘蓝叶绿体基因组定点整合表达载体pIKAA中。该载体以TrnA 和TrnI基因片段作为同源整合片段,能整合到CMS结球甘蓝叶绿体基因组中。此外,该载体是双顺反子形式的,即在转录的单条mRNA上,同时包含了K IN和aadA 基因编码区。将pIKAA转化到大肠杆菌中,结果显示转化有该载体的大肠杆菌能够在含有氨苄青霉素(AMP)和壮观霉素(SPEC)的固体LB平板中生长。研究结果可为后期CMS结球甘蓝叶绿体基因组的遗传转化体系的建立奠定基础。%To construct chloroplast site-specific integration expression vector is the first step for carrying on genetic transformation of cabbage chloroplast genome (Brassica oleracea L. var. capitata L.). In this study, antifree-ze protein KIN gene was cloned from cytoplasmic male sterility (CMS) cabbage (Brassica oleracea L. var. capitata L.), and was located in 2 chromosome in B. oleracea genome. By constructing the intermediate vector pKA and pAI, coding region of KIN gene was inserted into the site-specific integration expression vector (pIKAA) of CMS cabbage chloroplast. Due to the fragments of TrnA and TrnI used as homologous integration fragments, the pIKAA could target to chloroplast genomes of CMS cabbage. In addition, the pIKAA vector was bicistronic. The single transcribed mRNA from the pIKAA vector contained simultaneously coding regions of KIN and aadA gene. The vector was transformed into E. coli that can grow in LB containing ampicillin and spectinomycin. The study might lay essential basis in establishment of genetic transformation system of chloroplast genome of CMS cabbage.

  11. Cloning and Expression of an Ogura Cytoplasmic Male Sterile (OguCMS)-related MYB Transcription Factor in Brassica oleracea var.capitata%甘蓝胞质雄性不育(OguCMS)相关的MYB转录因子BoMYB1的克隆与表达分析

    Institute of Scientific and Technical Information of China (English)

    张磊; 康宗利; 刘海霞; 康俊根

    2012-01-01

    Ogura cytoplasmic male sterile (OguCMS) is the most widely used male sterile type in cabbage breeding. MYB transcription factors play a key role in regulation of plant defense response and multiple development processes. In present experiment, a R2R3-MYB transcription factor which down regulated 10.3 times in cabbage (Brasska okracea var. capitata) OguCMS lines was cloned by SMART RACE strategy.The full-length cDNA of B0MYB1 was 1 141 bp, which contained a 196 bp long 5' untranslated region, a 246 bp long 3' untranslated region and a 699 bp long open reading frame (GenBank accession number: JN703995). It was localized in the nucleus by subcellular localization prediction. It was an anther preferentially expressed gene in cabbage, which reached its expression peak in the late development. It was induced by the regulation of plant hormones salicylic acid(SA) and jasmonate methyl (JA-ME), and consequently regulated the expression of anther development genes. The experimental results suggests that B0MYB1 may be one of the important genes which involved in OguCMS anther development.%萝卜胞质雄性不育(OguCMS)是目前甘蓝中应用较广的雄性不育类型,MYB转录因子具有调控植物防御应答反应和多个发育过程的作用.本实验以在甘蓝(Brassica oleracea var.capitata)OguCMS花药中下调10.3倍的EST序列为信息探针,结合电子克隆及RACE技术,得到一个与甘蓝OguCMS雄性不育相关的MYB转录因子全长cDNA,命名为BoMYB1(GenBank登录号:JN703995).经亚细胞定位预测,该基因定位于细胞核,全长1 141 bp,包含一个长度为196 bp的5’非翻译区、246 bp的3’非翻译区和一个699 bp的开放阅读框.该基因在花药中具表达优势,并在花药发育晚期出现表达高峰,受植物激素水杨酸(SA)和茉莉酸甲酯(JA-ME)的调控,诱导花药发育基因的表达.实验结果提示,BoMY B1可能是参与OguCMS花药发育的重要基因之一.

  12. Wie kann der adäquate ärztliche Umgang mit Emotionen im Medizinstudium vermittelt werden? Ein Erfahrungsbericht aus dem Strang "Ärztliche Interaktion" im Modellstudiengang Medizin der Ruhr-Universität Bochum [How to teach the adequate handling of emotions during medical studies? A field report on "Medical Interaction" as part of a problem-based curriculum at the Ruhr University in Bochum, Germany

    Directory of Open Access Journals (Sweden)

    Rusche, Herbert

    2009-08-01

    Full Text Available [english] Handling strong emotions like sorrow, anger, shame, and fear in patients with somatic disease in an adequate way is a key issue for the future physician. Up to now, this aspect of the doctor?patient relationship has played a minor role in medical studies. To address this shortcoming, the handling of emotions is the focus of the third semester in the “Medical Interaction” course of the Modellstudiengang Medizin, the problem-based track of the medical faculty in Bochum, Germany. The course presented is given by the Department of General Medicine.The main objective is the recognition and correct interpretation of patients’ emotional expressions. The course is composed of six modules (one introductory and one for each leading affect. The main teaching methods are realistic role plays in a small group setting with video feedback, whereby one main requirement is that each student plays the role of both the patient and the doctor. Each session is introduced and analyzed by a general practitioner experienced in psychosomatic medicine. Evaluation of the course has shown a high acceptance among students. This motivates us to conduct a public discussion on the possibilities of applying this concept in other medical studies as well. [german] Der adäquate Umgang mit Emotionen wie Trauer, Wut, Scham und Angst bei Patienten mit körperlichen Erkrankungen spielt für den werdenden Arzt eine Schlüsselrolle. Dieser Aspekt der Arzt-Patient-Beziehung wird allerdings im Medizinstudium bislang wenig berücksichtigt. Aus diesem Grunde wird der Umgang mit Emotionen im dritten Semester des Stranges „ärztliche Interaktion“ im Modellstudiengang Medizin der Ruhr-Universität Bochum thematisiert. Der vorgestellte Kurs wird von der Abteilung für Allgemeinmedizin durchgeführt.Zielsetzung ist die Verbesserung der emotionalen und kommunikativen Kompetenz des Studierenden. Um dies zu erreichen soll dem eigenen emotionalen Erleben in der Arzt

  13. Control of medfly by SIT in the Nereva river valley

    Energy Technology Data Exchange (ETDEWEB)

    Bjelis, Mario, E-mail: mario.bjelis@zzb.h [Institut for Plant Protection in Agriculture and Foresty of Republic of Croatia, Zagreb, Zvonimirova (Croatia); Ljubetic, Visnja [Ministry of Agriculture, Forestry and Watter Managment of Republic of Croatia, Zagreb (Croatia); Novosel, Nevenka [State Office for Nuclear Safety, Zagreb (Croatia)

    2006-07-01

    A feasibility study of medfly suppression by means of sterile males released program in the Neretva Vallley, Croatia, is presented. The increase of medfly infestation is considered, as almost all cultures of the region represent host plants for the insect. Environmental friendly methods such well developed SIT technique associated with other organic methods are mentioned as an option of no disruption of the present natural balance. Area study and strategy planning is briefly presented. Population dynamics of Ceratitis capitata in the different parts of the delta Neretva valley, during period 2002 - 2004 Year is reported. Medfly capture on selected locations with different host availability in Neretva river is studied. (MAC)

  14. 夏威夷岛果蝇的综合防治

    Institute of Scientific and Technical Information of China (English)

    夏禹

    2003-01-01

    @@ 夏威夷地区受温暖潮湿气候的影响,外来引进的水果和蔬菜几乎全年都处在生长茂盛期.然而,一部分亚热带果蝇害虫也同样始终处于繁育旺期,其中包括桔小实蝇(Chaetodacus dorsalis)、甜瓜实蝇(Bactroceral latifrons)、地中海实蝇(Ceratitis capitata)和茄科(马来西亚)果蝇(Bactocerala tifrons).

  15. The importance of recognizing and reporting sequence database contamination for proteomics

    Directory of Open Access Journals (Sweden)

    Olivier Pible

    2014-06-01

    Full Text Available Advances in genome sequencing have made proteomic experiments more successful than ever. However, not all entries in a sequence database are of equal quality. Genome sequences are contaminated more frequently than is admitted. Contamination impacts homology-based proteomic, proteogenomic, and metaproteomic results. We highlight two examples in the National Center for Biotechnology Information non-redundant database (NCBInr that are likely contaminated: the bacterium Enterococcus gallinarum EGD-AAK12 and the insect Ceratitis capitata. We hope to incite users of this and other databases to critically evaluate submitted sequences and to contribute to the overall quality of the database by signaling potential errors when possible.

  16. Análise faunêstica de moscas-das-frutas (diptera, tephritidae em pomares de pessegueiro em Porto Alegre, Rio Grande do Sul Faunal study of fruit flies (Diptera, Tephritidae in peach or-chards in Porto Alegre, Rio Grande do Sul

    Directory of Open Access Journals (Sweden)

    Flávio Roberto Mello Garcia

    1998-12-01

    Full Text Available In order to characterizc the peach trees ecosystem in Porto Alegre related to tephrilidea species, a faunal study was developed over the data of the collects of these insects with traps in tvvelve months. For the characterization of the communities, constancy, abundance and frequency index were determined. Relation between sex were obtained by correlation matrix, sexual proportion and sexual rates. Anastrepha fratercalus (Wiedemann, 1830 was abundant, constant and frequent, Ceratitis capitata (Wiedemann, 1824 was accessory and Anastrepha grandis (Macquart, 1845 accidental. A greatcr number of females than males was observed.

  17. Diets based on soybean protein for Mediterranean fruit fly

    Energy Technology Data Exchange (ETDEWEB)

    Sobrinho, Raimundo Braga [Embrapa Agroindustria Tropical, Rua Dra. Sara Mesquita, 2270, CEP 60511-110 Fortaleza, CE (Brazil)]. E-mail: braga@cnpat.embrapa.br; Caceres, Carlos; Islam, Amirul; Wornoayporn, Vivat [Food and Agriculture Organization (FAO), International Atomic Energy Agency (IAEA), Agriculture and Biotechnology Laboratory, A-2444 Seibersdorf (Austria)]. E-mail: C.Caceres@iaea.org; Enkerlin, Walter [Insect Pest Control Section, Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna (Austria)]. E-mail: W.Enkerlin@iaea.org

    2006-04-15

    The objective of this work was to develop suitable and economic diets for mass rearing Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae). Diets containing sugar beet bagasse, wheat bran, brewer yeast, and others with wheat bran and palletized soybean protein from Brazil were tested. Diets based on soybean protein have shown promising results regarding pupal recovery, pupal weight and adult emergence. Soybean bagasse in the form of pellets with 60% of protein can be a very important substitute for other expensive sources of protein. (author)

  18. Isolation, identification and determination of the biological activity of candidate fruit volatile components from Argania spinosa L. (Sapotacea)

    Energy Technology Data Exchange (ETDEWEB)

    Bakri, A., E-mail: bakri@ucam.ac.m [University Cadi Ayyad, Marrakech (Morocco). Fac. of Science Semlalia. Insect Biological Control Unit; Dueben, B.D.; Proveaux, A.T.; Heath, R.R., E-mail: rheath@saa.ars.usda.go [U.S. Department of Agriculture (USDA/REE-ARS), Miami, FL (United States). Agricultural Research Service

    2006-07-01

    This study provides detailed information on the diversity, abundance, guilds, host plant and host fly ranges, distribution, and taxonomic status of hymenopterous parasitoid species associated with Ceratitis capitata (Wiedemann) and Anastrepha spp. (A. fraterculus (Wiedemann) and A. schultzi Blanchard) in Argentina. Moreover, the article also argues future needs regarding the use of some parasitoid species as an alternative tool in fruit fly management programs of the National Fruit Fly Control and Eradication Program (PROCEM-Argentina). Data used for this work were obtained from numerous old and recent published articles on fruit fly parasitoids in Argentina. (author)

  19. Wie kommt mehr "Familie" in die "Work-Life-Balance"? – Familienorientierung in der medizinischen Aus-, Weiterbildung und Berufstätigkeit [How to add more "Family" to the Work-Life-Balance? – Family Friendliness in Medical Under- and Postgraduate Studies and the Workplace

    Directory of Open Access Journals (Sweden)

    De Ridder, Daniela

    2012-04-01

    Wettbewerb um exzellente Köpfe müssen sich schon vor dem Hintergrund des demographischen Wandels diesen für sie zum Teil noch neuen Herausforderungen stellen. Hohe Studienabbruchquoten unter Studierenden mit Kindern, steigender Mediziner(innenmangel und deutliche Abwanderungsraten, hohe Kinderlosigkeit unter Akademiker(innen erscheinen dabei als Indikatoren für einen dringenden Handlungsbedarf hin zu einer familienorientierten Hochschul- und Fakultätsstrategie.Wie aber können medizinische Fakultäten, Kliniken und (Lehr-Krankenhäuser ein familienorientiertes Profil gewinnen? Welche Akteurinnen und Akteure, welche Handlungsfelder des Hochschulmanagements sind für die Leitungs- und Entscheidungsstrukturen relevant? Welche exemplarischen Maßnahmen für eine familiengerechtere Gestaltung des Studien- und Arbeitsortes „Medizin“ könnten Erfolg versprechend sein? Die Unterrepräsentanz von Frauen beim wissenschaftlichen Nachwuchs stellt darüber hinaus eine zusätzliche Herausforderung dar, für die eine innovative Hochschulpolitik weitere Maßnahmen entwickeln sollte, um zukunftsfähig zu sein. Nachwuchsförderungsstrategien und Familienorientierung sind demnach entscheidende Faktoren für eine zukunftsorientierte Hochschulpolitik. Diese Faktoren sollten deshalb als Führungsstrategien begriffen werden, um Maßnahmen einzuleiten, die das Profil der Hochschule dahingehend (umgestalten. Voraussetzung für eine erfolgreiche Implementierung ist dabei ein holistisches Konzept, das zu grundlegenden Reformen der Hochschulstrukturen führt, die im Folgenden skizziert und anhand von Beispielen illustriert werden sollen.

  20. Neem derivatives are not effective as toxic bait for tephritid fruit flies.

    Science.gov (United States)

    Silva, M A; Bezerra-Silva, G C D; Vendramim, J D; Mastrangelo, T; Forim, M R

    2013-08-01

    Neem derivatives have been widely touted as replacements for pesticides. A feasible replacement of synthetic insecticides in the management of fruit flies could be to use neem products in baits. This study evaluated the bioactivity of neem (Azadirachta indica A. Juss) derivatives in bait for adults of Anastrepha fraterculus (Wiedemann) and Ceratitis capitata (Wiedemann). The estimated LCs50 values for A. fraterculus and C. capitata were 7,522 ppm (18.40 ppm of azadirachtin) and 1,368 ppm (3.35 ppm of azadirachtin), respectively, using an aqueous extract of neem seeds in bait after 10 d of experimentation. No significant differences in the mortality of A. fraterculus and C. capitata adults exposed to baits made from different extracts and neem oil were observed after 3 h or 2 or 6 d; differences among the treatments were observed only on the 10th day of the evaluation. We conclude that neem derivatives applied as a bait spray over citrus plants did not demonstrate a toxic effect on A. fraterculus and C. capitata. The reasons for the low efficacy of the neem bait on Tephritid fruit flies are discussed.

  1. Seasonality of the Mediterranean Fruit Fly (Diptera: Tephritidae) on Terceira and Sao Jorge Islands, Azores, Portugal

    Science.gov (United States)

    Pimentel, R.; Lopes, D.J.H.; Mexia, A.M.M.; Mumford, J.D.

    2017-01-01

    Population dynamics studies are very important for any area-wide control program as they provide detailed knowledge about the relationship of Medfly [Ceratitis capitata (Wiedemann)] life cycle with host availability and abundance. The main goal of this study is to analyse seasonality of C. capitata in Terceira and Sao Jorge Islands (Azores archipelago) using field and laboratory data collected during (2010–2014) CABMEDMAC (MAC/3/A163) project. The results from Sao Jorge Island indicate significantly lower male/female ratio than on Terceira Island. This is an important finding specially regarding when stablishing the scenario parameters for a sterile insect technique application in each island. The population dynamics of C. capitata are generally linked with host fruit availability and abundance. However, on Terceira Island fruit infestation levels are not synchronized with the trap counts. For example, there was Medfly infestations in some fruits [e.g., Solanum mauritianum (Scop.)] while in the nearby traps there were no captures at the same time. From this perspective, it is important to denote the importance of wild invasive plants, on the population dynamics of C. capitata, as well important to consider the possibility of having different densities of traps according to the characteristics of each area in order to improve the network of traps surveillance’s sensitivity on Terceira Island. PMID:28082349

  2. Moscas frugívoras (Diptera, Tephritoidea coletadas em Aquidauana, MS Frugivorous flies (Diptera, Tephritoidea collected in Aquidauana, MS

    Directory of Open Access Journals (Sweden)

    Sérgio Roberto Rodrigues

    2006-03-01

    Full Text Available No Brasil as moscas frugívoras são pragas importantes de frutas e hortaliças. O conhecimento da flutuação populacional dessas espécies em cada bioma é um importante requisito para a adoção de estratégia de controle de pragas nos agroecossistemas. O objetivo desse trabalho foi avaliar a diversidade de espécies de moscas-das-frutas infestantes de frutas silvestres e cultivadas em Aquidauana, MS. Vinte e uma espécies de frutas foram amostradas de fevereiro de 2003 a janeiro de 2004. As espécies de Tephritidae encontradas foram: Anastrepha striata Schiner, 1868, Anastrepha obliqua (Macquart, 1835 e Ceratitis capitata (Wiedemann, 1824. Os frugívoros Lonchaeidae e Muscidae encontrados foram: Neosilba sp. e Atherigona orientalis (Schiner, 1868, respectivamente. Um total de 2.568 moscas foram coletadas, das quais 2.394 representadas pela mosca-do-Mediterrâneo C. capitata. A associação entre moscas frugívoras e espécies de frutas é discutida.In Brazil, frugivorous flies represent important pests of fruits and vegetables. Information on populational fluctuation of these species in each biome is an important requirement for the adoption of a strategy of pest control in the agroecosystems. The objective of this paper is to assess the diversity of fruit fly species occurring in wild and cultived fruits in Aquidauana, MS. Twenty-nine fruit species were sampled from February 2003 to January 2004. The Tephritidae species recovered were: Anastrepha striata Schiner, 1868, Anastrepha obliqua (Macquart, 1835 and Ceratitis capitata (Wiedemann, 1824. The frugivorous Lonchaeidae and Muscidae recovered were: Neosilba sp. and Atherigona orientalis (Schiner, 1868, respectively. A total of 2.568 flies were obtained, from which 2.394 flies were the Mediterranean fruit fly, C. capitata. The association between frugivorous flies and the fruit species is discussed.

  3. Nanostructured composite materials of cerium oxide and barium cerate

    Science.gov (United States)

    Medvedev, D. A.; Pikalova, E. Yu.; Demin, A. K.; Khrustov, V. R.; Nikolaenko, I. V.; Nikonov, A. V.; Malkov, V. B.; Antonov, B. D.

    2013-02-01

    Nanosized powders with a composition of (1- x)Ce0.8Sm0.2O2-δ- xBace0.8Sm0.2O3-δ ( x = 0, 0.3, and 1) were obtained by self-ignition combustion synthesis (SICS) from the appropriate nitrates and various organic fuels (glycine, glycerol, citric acid, and a mixture of citric acid and ethylene glycol). The most finely dispersed powders formed when the concentration of the perovskite phase in the system decreased or when glycerol or citric acid-enthyleneglycol mixture was used as a fuel during SICS. A procedure for the preparation of powders and nanostructured ceramics was developed and their electric properties were studied.

  4. Preparation of Nd-doped barium cerate through different routes

    DEFF Research Database (Denmark)

    Chen, F.L.; Toft Sørensen, O.; Meng, G.Y.;

    1997-01-01

    BaCe0.9Nd0.1O3-delta was prepared through different routes: oxalate coprecipitation (OF), carbonate-oxide mixed by ball-milling (COB) and carbonate-oxide mixed by mortar/pestle (COM). The reaction process studied by DTA-TG and XRD showed that calcination of the precursor powders at T greater than...... density and open porosity indicated that the calcination temperature had a major influence on the sintering process for all the three routes. The calcined powders from OP and COB had small particle size, large surface area and good sinterability, while those from COM had large particle size, small surface...

  5. Augmentative Biological Control Using Parasitoids for Fruit Fly Management in Brazil

    Directory of Open Access Journals (Sweden)

    Flávio R. M. Garcia

    2012-12-01

    Full Text Available The history of classical biological control of fruit flies in Brazil includes two reported attempts in the past 70 years. The first occurred in 1937 when an African species of parasitoid larvae (Tetrastichus giffardianus was introduced to control the Mediterranean fruit fly, Ceratitis capitata and other tephritids. The second occurred in September 1994 when the exotic parasitoid Diachasmimorpha longicaudata, originally from Gainesville, Florida, was introduced by a Brazilian agricultural corporation (EMBRAPA to evaluate the parasitoid’s potential for the biological control of Anastrepha spp. and Ceratitis capitata. Although there are numerous native Brazilian fruit fly parasitoids, mass rearing of these native species is difficult. Thus, D. longicaudata was chosen due to its specificity for the family Tephritidae and its ease of laboratory rearing. In this paper we review the literature on Brazilian fruit fly biological control and suggest that those tactics can be used on a large scale, together creating a biological barrier to the introduction of new fruit fly populations, reducing the source of outbreaks and the risk of species spread, while decreasing the use of insecticides on fruit destined for domestic and foreign markets.

  6. Species of fruit flies (Tephritidae obtained of McPhail trap in the Bahia State, Brazil/ Espécies de moscas-das-frutas (Tephritidae obtidas em armadilhas McPhail no Estado da Bahia, Brasil

    Directory of Open Access Journals (Sweden)

    Miguel Francisco de Souza Filho

    2006-07-01

    Full Text Available The objective of this study was to provide knowledge on the species of fruit flies in commercial orchards in counties of the southern and extreme southern regions of the State of Bahia, Brazil. Flies were captured weekly by McPhail traps, using a hydrolyzed corn protein at 5%, as attractant. A total of 257 female was collected, and the species were identified as: Anastrepha fraterculus (77.4%, A. sororcula (4.7%, A. obliqua (2.7%, A. zenildae (0.8%, A. distincta (0.4%, A. consobrina (0.4%, Anastrepha sp.1 (5.1% and Ceratitis capitata (8.5%.O objetivo deste trabalho foi conhecer as espécies de moscas-das-frutas que ocorrem em pomares comerciais em alguns municípios da região sul e extremo-sul do estado da Bahia, Brasil. As moscas-dasfrutas foram capturadas, semanalmente, utilizando-se armadilhas McPhail, tendo como atrativo proteína hidrolisada de milho a 5%. Foi obtido um total de 257 espécimes fêmeas, pertencentes às espécies: Anastrepha fraterculus (77,4%, A. sororcula (4,7%, A. obliqua (2,7%, A. zenildae (0,8%, A. distincta (0,4%, A. consobrina (0,4%, Anastrepha sp.1 (5,1% e Ceratitis capitata (8,5%.

  7. Diets based on soybean protein for Mediterranean fruit fly Dietas baseadas em proteína de soja para moscas do Mediterrâneo

    Directory of Open Access Journals (Sweden)

    Raimundo Braga Sobrinho

    2006-04-01

    Full Text Available The objective of this work was to develop suitable and economic diets for mass rearing Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae. Diets containing sugar beet bagase, wheat bran, brewer yeast, and others with wheat bran and palletized soybean protein from Brazil were tested. Diets based on soybean protein have shown promising results regarding pupal recovery, pupal weight and adult emergence. Soybean bagase in the form of pellets with 60% of protein can be a very important substitute for other expensive sources of protein.O objetivo deste trabalho foi desenvolver dietas adequadas e econômicas para a criação massal de moscas de frutas do Mediterrâneo, Ceratitis capitata (Diptera: Tephritidae. Foram testados dietas com bagaço de beterraba açucareira, farelo de trigo, levedura de cerveja e outras dietas de farelo de trigo e proteína de soja prensada brasileira. Dietas compostas por proteína de soja apresentaram resultados positivos de recuperação de pupas, pesos de pupa e emergência de adultos. O bagaço de soja, na forma de pellet com 60% de proteína, pode ser um importante substituto de outras fontes de proteína.

  8. Augmentative Biological Control Using Parasitoids for Fruit Fly Management in Brazil.

    Science.gov (United States)

    Garcia, Flávio R M; Ricalde, Marcelo P

    2012-12-21

    The history of classical biological control of fruit flies in Brazil includes two reported attempts in the past 70 years. The first occurred in 1937 when an African species of parasitoid larvae (Tetrastichus giffardianus) was introduced to control the Mediterranean fruit fly, Ceratitis capitata and other tephritids. The second occurred in September 1994 when the exotic parasitoid Diachasmimorpha longicaudata, originally from Gainesville, Florida, was introduced by a Brazilian agricultural corporation (EMBRAPA) to evaluate the parasitoid's potential for the biological control of Anastrepha spp. and Ceratitis capitata. Although there are numerous native Brazilian fruit fly parasitoids, mass rearing of these native species is difficult. Thus, D. longicaudata was chosen due to its specificity for the family Tephritidae and its ease of laboratory rearing. In this paper we review the literature on Brazilian fruit fly biological control and suggest that those tactics can be used on a large scale, together creating a biological barrier to the introduction of new fruit fly populations, reducing the source of outbreaks and the risk of species spread, while decreasing the use of insecticides on fruit destined for domestic and foreign markets.

  9. The hydrocoral genus Millepora (Hydrozoa: Capitata: Milleporidae) in Indonesia

    NARCIS (Netherlands)

    Razak, T.B.; Hoeksema, B.W.

    2003-01-01

    This revision of Indonesian Millepora species is based on the morphology of museum specimens and photographed specimens in the field. Based on the use of pore characters and overall skeleton growth forms, which are normally used for the classification of Millepora, the present study concludes that s

  10. Diversity and seasonality of fruit flies (Diptera: Tephritidae and Lonchaeidae and their parasitoids (Hymenoptera: Braconidae and Figitidae in orchards of guava, loquat and peach

    Directory of Open Access Journals (Sweden)

    MF. Souza-Filho

    Full Text Available This work was carried out in orchards of guava progenies, and loquat and peach cultivars, in Monte Alegre do Sul, SP, Brazil, in 2002 and 2003. Guavas and loquats were bagged and unbagged bi-weekly and weekly, respectively, for assessment of the infestation period. Peach was only bagged weekly. The assays started when the fruits were at the beginning of development, but still green. Ripe fruits were taken to the laboratory and placed individually into plastic cups. McPhail plastic traps containing torula yeast were hung from January 2002 to January 2004 to assess the fruit fly population in each orchard, but only the Ceratitis capitata population is here discussed. Five tephritid species were reared from the fruits: Anastrepha bistrigata Bezzi, A. fraterculus (Wiedemann, A. obliqua (Macquart, A. sororcula Zucchi, and C. capitata, in addition to six lonchaeid species: Neosilba certa (Walker, N. glaberrima (Wiedemann, N. pendula (Bezzi, N. zadolicha McAlpine and Steyskal, Neosilba sp. 4, and Neosilba sp. 10 (both species are in the process of being described by P. C. Strikis, as well as some unidentified Neosilba species. Ten parasitoid species were obtained from fruit fly puparia, of which five were braconids: Asobara anastrephae (Muesebeck, Doryctobracon areolatus (Szépligeti, D. brasiliensis (Szépligeti, Opius bellus Gahan, and Utetes anastrephae (Viereck, and five figitids: Aganaspis pelleranoi (Brèthes, Dicerataspis grenadensis Ashmead, Lopheucoila anastrephae (Rhower, Leptopilina boulardi (Barbotin, Carlton and Kelner-Pillaut, and Trybliographa infuscata Diaz, Gallardo and Uchôa. Ceratitis capitata showed a seasonal behavior with population density peaking at the second semester of each year. Anastrepha and Neosilba species remained in the orchards throughout both years.

  11. Dispersal capacity of fruit fly parasitoid Diachasmimorpha longicaudata (Hymenoptera: Braconidae in irrigated coffee plantations

    Directory of Open Access Journals (Sweden)

    Maria Gisely Camargos

    2016-06-01

    Full Text Available ABSTRACT Diachasmimorpha longicaudata is an Old World parasitoid of tephritid fruit flies that was widely introduced in the Americas to control pest species such as the Mediterranean fruit fly Ceratitis capitata. Augmentative releases in irrigated coffee plantations in semiarid regions of Brazil are under consideration and dispersal capacity of D. longicaudata in this habitat are important to develop release strategies. Approximately 2,000 individuals of D. longicaudata (5 to 7 days old were released in the center of a fruiting coffee plantation every two weeks from Dec. 2009 to Apr. 2010. Dispersal from the central release point was monitored to the north, south, east, west, northeast, northwest, southeast and southwest at 11 distances, beginning at 4.6 m and ending at 90 m from the release point. At each point, a parasitism unit (approximately 120 larvae of C. capitata in the 3rd instar wrapped in voile fabric and 10 coffee beans were collected. The average dispersion distance and dispersion area were estimated by the model proposed by Dobzhansky and Wright (1943. The average dispersion distances were 27.06 m (as estimated by fruit collection and 33.11 m (as estimated by oviposition traps. The average dispersion areas were 1,315.25 m2 and 1,752.45 m2 originating from the collection of beans and parasitism units, respectively. Cohorts of 2,000 adult D. longicaudata released at six points ha−1 are estimated to result in sufficient colonization to exert significant control of Ceratitis capitata.

  12. Diversity and seasonality of fruit flies (Diptera: Tephritidae and Lonchaeidae) and their parasitoids (Hymenoptera: Braconidae and Figitidae) in orchards of guava, loquat and peach

    Energy Technology Data Exchange (ETDEWEB)

    Souza-Filho, M.F.; Raga, A. [Instituto Biologico, Campinas, SP (Brazil)], e-mail: miguelf@biologico.sp.gov.br; Azevedo-Filho, J.A. [Agencia Paulista de Tecnologia dos Agronegocios (APTA), Monte Alegre do Sul, SP (Brazil). Polo Regional do Leste Paulista; Strikis, P.C. [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Inst. de Biologia. Dept. de Parasitologia; Guimaraes, J.A. [EMBRAPA Agroindustria Tropical, Fortaleza, CE (Brazil); Zucchi, R.A. [Escola Superior de Agricultura Luiz de Queiroz (ESALQ/USP), Piracicaba, SP (Brazil). Dept. de Entomologia, Fitopatologia e Zoologia Agricola

    2009-02-15

    This work was carried out in orchards of guava progenies, and loquat and peach cultivars, in Monte Alegre do Sul, SP, Brazil, in 2002 and 2003. Guavas and loquats were bagged and unbagged bi-weekly and weekly, respectively, for assessment of the infestation period. Peach was only bagged weekly. The assays started when the fruits were at the beginning of development, but still green. Ripe fruits were taken to the laboratory and placed individually into plastic cups. McPhail plastic traps containing torula yeast were hung from January 2002 to January 2004 to assess the fruit fly population in each orchard, but only the Ceratitis capitata population is here discussed. Five tephritid species were reared from the fruits: Anastrepha bistrigata Bezzi, A. fraterculus (Wiedemann), A. obliqua (Macquart), A. sororcula Zucchi, and C. capitata, in addition to six lonchaeid species: Neosilba certa (Walker), N. glaberrima (Wiedemann), N. pendula (Bezzi), N. zadolicha McAlpine and Steyskal, Neosilba sp. 4, and Neosilba sp. 10 (both species are in the process of being described by P. C. Strikis), as well as some unidentified Neosilba species. Ten parasitoid species were obtained from fruit fly puparia, of which five were braconids: Asobara anastrephae (Muesebeck), Doryctobracon areolatus (Szepligeti), D. brasiliensis (Szepligeti), Opius bellus Gahan, and Utetes anastrephae (Viereck), and five figitids: Aganaspis pelleranoi (Brethes), Dicerataspis grenadensis Ashmead, Lopheucoila anastrephae (Rhower), Leptopilina boulardi (Barbotin, Carlton and Kelner-Pillaut), and Trybliographa infuscata Diaz, Gallardo and Uchoa. Ceratitis capitata showed a seasonal behavior with population density peaking at the second semester of each year. Anastrepha and Neosilba species remained in the orchards throughout both years. (author)

  13. Vertically transmitted rhabdoviruses are found across three insect families and have dynamic interactions with their hosts

    Science.gov (United States)

    Day, Jonathan P.; Schulz, Nora; Leftwich, Philip T.; de Jong, Maaike A.; Wilfert, Lena; Smith, Sophia C. L.; McGonigle, John E.; Houslay, Thomas M.; Livraghi, Luca; Evans, Luke C.; Friend, Lucy A.; Vontas, John; Kambouraki, Natasa

    2017-01-01

    A small number of free-living viruses have been found to be obligately vertically transmitted, but it remains uncertain how widespread vertically transmitted viruses are and how quickly they can spread through host populations. Recent metagenomic studies have found several insects to be infected with sigma viruses (Rhabdoviridae). Here, we report that sigma viruses that infect Mediterranean fruit flies (Ceratitis capitata), Drosophila immigrans, and speckled wood butterflies (Pararge aegeria) are all vertically transmitted. We find patterns of vertical transmission that are consistent with those seen in Drosophila sigma viruses, with high rates of maternal transmission, and lower rates of paternal transmission. This mode of transmission allows them to spread rapidly in populations, and using viral sequence data we found the viruses in D. immigrans and C. capitata had both recently swept through host populations. The viruses were common in nature, with mean prevalences of 12% in C. capitata, 38% in D. immigrans and 74% in P. aegeria. We conclude that vertically transmitted rhabdoviruses may be widespread in a broad range of insect taxa, and that these viruses can have dynamic interactions with their hosts. PMID:28100819

  14. Assessment of differences between X and gamma rays in order to validate a new generation of irradiators for insect sterilization

    Energy Technology Data Exchange (ETDEWEB)

    Mastrangelo, Thiago; Walder, Julio M.M., E-mail: piaui@cena.usp.b [Centro de Energia Nuclear na Agricultura (CENA), Piracicaba, SP (Brazil); Parker, Andrew G.; Jessup, Andrew; Orozco-Davila, Dina; Islam, Amirul; Dammalage, Thilakasiri, E-mail: A.Jessup@iaea.or [Joint FAO/IAEA-UN A-2444, Seibersdorf (Austria). Insect Pest Control Subprogramme; Pereira, Rui, E-mail: R.Cardoso-Pereira@iaea.or [Joint FAO/IAEA-UN, Vienna (Austria). Insect Pest Control Subprogramme

    2009-07-01

    Recent fears of terrorism provoked an increase in delays and denials of transboundary shipments of radioisotopes. This represents a serious constraint to sterile insect technique (SIT) programs around the world as they rely on the use of ionizing energy from radioisotopes for insect sterilization. In order to validate a novel Xray irradiator, a series of studies on Ceratitis capitata (Wiedemann) and Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae) were carried out, comparing the relative biological effectiveness (RBE) between X-rays and traditional gamma radiation from {sup 60}Co. Male C. capitata pupae and pupae of both sexes of A. fraterculus, both 24 to 48 h before adult emergence, were irradiated with doses ranging from 15 to 120 Gy and 10 to 70 Gy respectively. Estimated mean doses of 91.2 Gy of X and 124.9 Gy of gamma radiation induced 99% sterility in C. capitata males. Irradiated A. fraterculus were 99% sterile at about 40-60 Gy for both radiation treatments. Standard quality control parameters were not significantly affected by the two types of radiation. There were no significant differences between X and gamma radiation regarding mating indices. The RBE did not differ significantly between the tested X and gamma radiation, and X-rays are as biologically effective for SIT purposes as gamma rays are. This work confirms the suitability of this new generation of X-ray irradiators for pest control programs in UN Member States. (author)

  15. Chemosterilant bait stations coupled with sterile insect technique: an integrated strategy to control the Mediterranean fruit fly (Diptera: Tephritidae).

    Science.gov (United States)

    Navarro-Llopis, V; Vacas, S; Sanchis, J; Primo, J; Alfaro, C

    2011-10-01

    During 2008 and 2009, the efficacy of the combination of two Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), control techniques, sterile insect technique (SIT) and a chemosterilant bait station system (Adress), was tested in three crops: citrus (Citrus spp.), stone fruit (Prunus spp.), and persimmon (Diospyros spp.). Two thousand sterile males were released per ha each week in the whole trial area (50,000 ha, SIT area). For 3,600 ha, within the whole trial area, 24 Adress traps per ha were hung (SIT + Adress area). Ten SIT + Adress plots and 10 SIT plots in each of three different fruit crops were arranged to assess Mediterranean fruit fly population densities and fruit damage throughout the trial period. To evaluate the efficacy of each treatment, the male and female populations were each monitored from August 2008 to November 2009, and injured fruit was assessed before harvest. Results showed a significant reduction in the C. capitata population in plots treated with both techniques versus plots treated only with the SIT. Likewise, a corresponding reduction in the percentage of injured fruit was observed. These data indicate the compatibility of these techniques and suggest the possibility of using Adress coupled with SIT to reduce C. capitata populations in locations with high population densities, where SIT alone is not sufficiently effective to suppress fruit fly populations to below damaging levels.

  16. Controle químico de Anastrepha fraterculus (Wied. (Diptera: Tephritidae em laboratório Chemical control of Anastrepha fraterculus (Wied. (Diptera: Tephritidae in laboratory

    Directory of Open Access Journals (Sweden)

    Priscila Lang Scoz

    2004-12-01

    Full Text Available O efeito de quatro novos grupos químicos de inseticidas incluindo avermectina (benzoato de emamectina, éter piretróide (etofemprox, neoniconitnóide (imidacloprid, thiacloprid e thiamethoxan e naturalyte (spinosad foram avaliados em laboratório (25 ± 3ºC, umidade relativa de 70 ± 10% e fotofase de 12 horas, visando ao controle de adultos e ovos/larvas de Anastrepha fraterculus comparando-os com os fosforados fenthion e thrichlorphon. O benzoato de emamectina não foi eficiente no controle de adultos de A. fraterculus via contato e ingestão. O etofenprox, imidacloprid, spinosad e thiamethoxan foram eficientes no controle de adultos de A. fraterculus via contato e ingestão, proporcionando maior mortalidade via ingestão. Os novos inseticidas não provocaram mortalidade significativa de ovos/larvas de A. fraterculus localizados no interior de maçãs, enquanto que os fosforados fenthion e thrichlorphon resultaram em 100% de mortalidade das fases imaturas e adultos. Os novos inseticidas apresentam potencial para uso nas iscas tóxicas, substituindo os fosforados para o controle de adultos.The South American Fruit Fly, Anastrepha fraterculus is one of the most important pest of temperate fruit crops. The effect of four new inseticide groups to replace organophosphate compounds for A. fraterculus control was evaluated under laboratory conditions (25 ± 3ºC, relative humity of 70 ± 10% and 12:12 L:D. Emamectin benzoate, etofenprox, imidacloprid, spinosad, thiacloprid and thiamethoxan were evaluated to control adults by contact and ingestion and against eggs/larvae inside apple fruits compared with fenthion and thrichlorphon. Emamectin benzoate was not efficient to control adults of A. fraterculus by contact and ingestion. Etofenprox, imidacloprid, spinosad and thiamethoxan were efficient to control adults by contact and ingestion being more toxic by ingestion. No new insecticide controlled eggs/larvae inside apple fruit while organophosphate compounds caused 100% of mortality of immature stages and adults. The new insecticides showed more potential to substitute organophosphate compounds in bait sprays for adult control.

  17. First register of fruit flies (Diptera: Tephritidae in star fruit in Teresina, Altos and Parnaiba, state of Piaui, Brazil/ Primeiro registro de moscas-das-frutas (Diptera: Tephritidae em carambola nos municípios de Teresina, Altos e Parnaíba no estado do Piauí

    Directory of Open Access Journals (Sweden)

    Almerinda Amélia Rodrigues Araújo Soares

    2007-08-01

    Full Text Available The present work aims to register the occurrence of the fruit flies associated to star fruit (Averrhoa carambola L. in three counties of the state of Piaui, as well as to determine the frequency and the index of infestation of these insects. The fruits had been collected during the months of August and September 2005, and had been placed in plastic trays with sterilized soil, stored in metal cages, and left in environmental temperature at the laboratory. Until emergency, the adults had been kept in bottles with alcohol 70% and later identified in the species level. The biggest index of infestation (flies/fruit of C. capitata has occurred in the county of Altos (3.66, followed by Teresina and Parnaiba that had presented index of infestation of 2.18 and 0.016, respectively. C. capitata was the most frequent species in all the counties, presenting frequencies of 100%, 96.5%, and 100% in Teresina, Altos and Parnaiba, respectively. Ceratitis capitata is registered for the first time in star fruit in Teresina, Altos and Parnaiba, state of the Piaui. Anastrepha fraterculus is registered for the first time in the county of Altos. A. fraterculus and C. capitata occur simultaneously in star fruits.O presente trabalho visou conhecer as espécies de moscas-das-frutas associadas à carambola (Averrhoa carambola L. em três municípios do Estado do Piauí, bem como determinar a freqüência e o índice de infestação desses insetos. Os frutos foram coletados durante os meses de agosto e setembro de 2005, colocados em bandejas plásticas com solo esterilizado, armazenados em gaiolas metálicas e deixados em temperatura ambiente no laboratório. Até a emergência dos adultos, estes foram acondicionados em frascos contendo álcool 70% e posteriormente identificados em nível de espécie. O maior índice de infestação (moscas/fruto de C. capitata ocorreu no município de Altos (3,66, seguido pelos municípios de Teresina e Parnaíba que apresentaram

  18. Genetics and biology of Anastrepha fraterculus: research supporting the use of the sterile insect technique (SIT) to control this pest in Argentina.

    Science.gov (United States)

    Cladera, Jorge L; Vilardi, Juan C; Juri, Marianela; Paulin, Laura E; Giardini, M Cecilia; Gómez Cendra, Paula V; Segura, Diego F; Lanzavecchia, Silvia B

    2014-01-01

    Two species of true fruit flies (taxonomic family Tephritidae) are considered pests of fruit and vegetable production in Argentina: the cosmopolitan Mediterranean fruit fly (Ceratitis capitata Wiedemann) and the new world South American fruit fly (Anastrepha fraterculus Wiedemann). The distribution of these two species in Argentina overlaps north of the capital, Buenos Aires. Regarding the control of these two pests, the varied geographical fruit producing regions in Argentina are in different fly control situations. One part is under a programme using the sterile insect technique (SIT) for the eradication of C. capitata, because A. fraterculus is not present in this area. The application of the SIT to control C. capitata north of the present line with the possibility of A. fraterculus occupying the niche left vacant by C. capitata becomes a cause of much concern. Only initial steps have been taken to investigate the genetics and biology of A. fraterculus. Consequently, only fragmentary information has been recorded in the literature regarding the use of SIT to control this species. For these reasons, the research to develop a SIT protocol to control A. fraterculus is greatly needed. In recent years, research groups have been building a network in Argentina in order to address particular aspects of the development of the SIT for Anastrepha fraterculus. The problems being addressed by these groups include improvement of artificial diets, facilitation of insect mass rearing, radiation doses and conditions for insect sterilisation, basic knowledge supporting the development of males-only strains, reduction of male maturation time to facilitate releases, identification and isolation of chemical communication signals, and a good deal of population genetic studies. This paper is the product of a concerted effort to gather all this knowledge scattered in numerous and often hard-to-access reports and papers and summarize their basic conclusions in a single publication.

  19. Biodiversidade de moscas-das-frutas (Diptera, Tephritoidea em matas nativas e pomares domésticos de dois municípios do Estado do Tocantins, Brasil Biodiversity of fruit flies (Diptera, Tephritoidea in native forests and orchards in two counties of the State of Tocantins, Brazil

    Directory of Open Access Journals (Sweden)

    Darcy A. do Bomfim

    2007-01-01

    Full Text Available Este trabalho apresenta análise faunística comparativa das espécies de moscas-das-frutas capturadas em armadilhas McPhail (junho a dezembro de 2002 com proteína hidrolisada de milho a 5%. Foram comparadas a riqueza de espécies e a estrutura populacional entre ambientes de mata e pomar dos municípios de Palmas e Porto Nacional, TO. Foram capturados 1.748 indivíduos de espécies de três gêneros de Tephritidae: Tomoplagia Coquillett, 1910, Anastrepha Schiner, 1868 e Ceratitis MacLeay, 1829. De Lonchaeidae foram capturadas espécies de três gêneros: Lonchaea Fallén, 1820, Neosilba McAlpine, 1962 e Dasiops Rondani, 1856. Ceratitis capitata (Wiedemann, 1824. Dezenove espécies de Anastrepha foram coletadas, sendo a maioria dos indivíduos (69,1% de A. obliqua (Macquart, 1835. Não houve diferença significativa (P This paper presents comparative and faunistic analysis of the species of fruit flies captured in McPhail traps (from June to December 2002 baited with 5% corn protein hydrolyzed. Species richness and the patterns of population are compared between forest and orchard environments and between the counties of Palmas and Porto Nacional. A total of 1,748 individuals of Tephritidae belonging to species of three genera were collected: Tomoplagia Coquillett, 1910, Anastrepha Schiner, 1868 and Ceratitis MacLeay, 1829. Species of three genera of Lonchaeidae were also captured: Lonchaea Fallén, 1820, Neosilba McAlpine, 1962 and Dasiops Rondani, 1856. Ceratitis capitata (Wiedemann, 1824 and nineteen species of the genus Anastrepha were collected. Most of the collected individuals (69.1% belonged to A. obliqua (Macquart, 1935. The average numbers of tephritid individuals in Palmas and native forests were significantly lower than Porto Nacional and orchards, respectively. According to the Shannon diversity index (H' and test t used for comparing the fruit flies fauna among the environments, it was verified that only one comparison showed

  20. Jurisprudence. Sufferance tax. Who pays the bill?; Jurisprudentie. Precariobelasting. Wie betaalt de rekening?

    Energy Technology Data Exchange (ETDEWEB)

    Brinkman, I.; Roggenkamp, M. [Brinkhof Advocaten, Amsterdam (Netherlands)

    2011-10-15

    Network operators in the Netherlands must pay sufferance taxes for using the municipal surface. This follows from a decision of the Dutch Supreme Court on a matter between the grid operator Liander and the municipality Leiden. Network operators can charge the cost for such services to the customers. [Dutch] Netbeheerders moeten precariobelasting betalen voor het gebruik van de gemeentelijke ondergrond. Dat volgt uit een uitspraak van de Hoge Raad over een zaak tussen netbeheerder Liander en de gemeente Leiden. Netbeheerders kunnen de precariolasten uiteindelijk in rekening brengen bij de afnemers.

  1. Waiting for warm water - how long? Pipelines; Warten auf warmes Wasser - wie lange? Rohrleitung

    Energy Technology Data Exchange (ETDEWEB)

    Hofmann, M.

    2006-11-15

    How long should it take until warm water is available at the tap? Since 2004, this question has been discussed in the catalogue of technical rules, with varying answers. This contribution outlines the current situation and presents some thoughts of the author that highlight new aspects. Practical hints are given for daily practice. (orig.)

  2. Primer registro de Tiaris fuliginosa (Wied, 1830) para la Argentina

    OpenAIRE

    Mazar Barnett, Juan; Herrera, Justo

    1996-01-01

    El primer registro de Argentina de Tiaris fuliginosa , basado en dos pájaros encontrados en el Parque Nacional Iguazú. The first record for Argentina of the Sooty Grassquit ( Tiaris fuliginosa ) is given, based on two birds notted at Iguazú National Park.

  3. Kenneth Gergen: Die "Oldstream"-Psychologie wird verschwinden wie die Dinosaurier!

    OpenAIRE

    Mattes, Peter; Schraube, Ernst

    2004-01-01

    Das Gespräch beleuchtet das Wissenschaftsverständnis des sozialen Konstruktionismus, Theorie – Methodik – Praxis, im Spannungsfeld zur traditionellen Psychologie. Der erste Teil handelt von Orten und Formen sozialkonstruktionistischen Denkens und es werden Grenzen des herkömmlichen psychologischen Wissenschaftsverständnisses erörtert. Danach geht es um die Frage einer spezifisch sozialkonstruktionistischen Methodologie und um die Bedeutung sozialer Beziehungen für ein angemessenes Verständnis...

  4. Zorg; wie doet er wat aan : Een studie naar zorgarrangementen van ouderen

    NARCIS (Netherlands)

    Luijkx, K.G.

    2001-01-01

    As a result of fertility decline and increasing life expectancy, the age structure of the Dutch population is changing, with a larger proportion and number of elderly persons and declining proportions and numbers of younger adults. Hence, the number of people in need of care is growing. While -at th

  5. Wie leistungsfähig sind die Ökonomien Afrikas?

    OpenAIRE

    Pohl, Birte; Kappel, Robert

    2012-01-01

    Der IWF prognostiziert in seinem "Regional Economic Outlook: Sub-Saharan Africa" vom Oktober 2012 für Afrika weiterhin ein hohes Wachstum von 5 bis 6 Prozent für die Jahre 2012 und 2013. Anders als bei den zahlreichen pauschal-optimistischen Einschätzungen zur wirtschaftlichen Entwicklung Afrikas wird hier auf der Grundlage des Performanzindex Afrika (PIA) eine sehr viel differenziertere und weniger optimistische Analyse des Entwicklungspotenzials afrikanischer Volkswirtschaften vorgeste...

  6. Complex trauma en complexe PTSS: Wat is het en wie heeft het?

    NARCIS (Netherlands)

    ter Heide, J.J.; Kleber, Rolf; Mooren, G.T.M.

    2014-01-01

    Er bestaat onder clinici veel onduidelijkheid over wat de concepten complex trauma en complexe posttraumatische stressstoornis (PTSS) behelzen. Dit heeft ongewenste consequenties voor de behandeling, omdat de behandelrichtlijnen voor complexe PTSS afwijken van die voor PTSS. In dit artikel wordt ver

  7. Bipedal locomotion in Tropidurus torquatus (Wied, 1820 and Liolaemus lutzae Mertens, 1938

    Directory of Open Access Journals (Sweden)

    O. Rocha-Barbosa

    Full Text Available Bipedalism has evolved on numerous occasions in phylogenetically diverse lizard families. In this paper we describe, for the first time, bipedal locomotion on South American lizards, the sand-dweller Liolaemus lutzae and the generalist Tropidurus torquatus. The lizards were videotaped running on a racetrack and the sequences were analyzed frame by frame. The body posture, as a whole, diverged a lot during bipedal locomotion between the two species, even though there was no difference regarding their sprint performance. The locomotor behavior of L. lutzae is, in general, more similar to the one observed on other sand-dweller lizards. Certain particularities are common, such as the digitigrade posture at footfall and throughout stance, trunk angles; and tail posture. In contrast, T. torquatus exhibited high trunk angles and dragged its tail, in a posture compared to basilisks. This body posture could be related to certain characteristics and obstacles of a microhabitat such as the one around lakes and streams (basilisks and the one with compact shrubby vegetation (T. torquatus.

  8. 'Gewoon worden wie je bent': Over authenticiteit en anti-institutionalisme

    NARCIS (Netherlands)

    S.D. Aupers (Stef); D. Houtman (Dick); I. van der Tak

    2003-01-01

    textabstractAnalysis of a dozen semi-structured interviews with Dutch New Age trainers demonstrates that the modern 'ethics of authenticity' underlies contemporary society's anti-institutional mood. All institutions, be they traditional or modern, or so our respondants argue, prevent one from being

  9. Wie 'Haar' zu 'Maus' wird: Geschlechtsbezogene pronominale Referenz im Thailändischen

    Directory of Open Access Journals (Sweden)

    Attaviriyanupap, Korakoch

    2004-01-01

    Full Text Available The use of gender-related personal pronouns is mostly found in different forms of third person singular (e. g. he/she/it in English or er/sie/es in German. In Thai, an isolate language without grammatical gender, the gender-specification can be observed in the use of first person singular pronominal reference. The selection of gender-related self-reference depends not only on sex but also on gender identity of the speaker. In this article, the complex system of pronominal reference in Thai language will be briefly introduced. After that, forms of self-reference used in the Thai film Iron Ladies are analyzed to show how the gender identity of speakers can influence their selection of pronominal reference.

  10. Von Mr Classic zu Mr Nerd: Wie Forschende soziale Medien nutzen

    Directory of Open Access Journals (Sweden)

    Doreen Siegfried

    2014-12-01

    Full Text Available Unter Wissenschaftlerinnen und Wissenschaftlern lassen sich vier Typen differenzieren, die ein signifikant unterschiedliches Verhalten in ihrer Nutzung und ihrer Einstellung gegenüber Social-Web-Anwendungen zeigen: Ms Maker, Mr Tech, Mr Classic und Mr Nerd. Grundlage für die Identifizierung dieser Social-Media-Typen ist die Auswertung von 778 Online-Fragebögen, die von Anfang September bis Mitte Oktober 2013 erhoben wurden. Erfragt wurde die Nutzung von Social-Media-Instrumenten für die tägliche Arbeit in Forschung, Lehre, Administration und Wissenschaftskommunikation. Neben Intensität und Kontext der Nutzung von Web-2.0-Diensten wurden auch die Gründe für Nutzung bzw. Nicht-Nutzung einzelner Kanäle sowie generelle Einstellungen gegenüber Social-Media-Werkzeugen erfragt. Among scientists, four types of personality can be identified whose usage and attitude towards social web applications show significant variations: Ms Maker, Mr Tech, Mr Classic and Mr Nerd. This typification is based on 778 online questionnaires received between September and October 2013. The survey sampled the usage of social media tools for daily routines in research, teaching, administrative work and scholarly communication. The survey asked questions about the intensity and the context in which Web 2.0 services are used, about the reasons for (not using certain channels, and about general attitudes towards social media tools.

  11. Wie Achim zu einem Himbarchen wurde (How Achim Became a Raspberry).

    Science.gov (United States)

    Frank, Karlhans

    This storybook/workbook presents a communicative-based approach to learning German as a foreign language. It is intended for developing the reading skills of the young learner of German in Alberta. The text concerns the adventures of a child. Interspersed throughout the story are various exercises in which the learner is engaged to fill in or…

  12. How warm was the year 2010? Background; Wie warm war das Jahr 2010? Hintergrund

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2011-05-06

    In the background paper under consideration, the Federal Environment Agency (Dessau-Rosslau, Federal Republic of Germany) reports on a global measurement of the surface air temperature in order to obtain a global mean temperature. The global mean temperature of the year 2010 is not very significant. The deviation of the global mean temperature of the year 2010 from the mean temperature in a recent, extended period of time is more significant. The long-term trend in the global mean temperature shows a progressive global warming. The year 2010 was the warmest calendar year with the largest amount of rainfall since the records began in the 19th century. The global mean surface air temperature was very slight above the average temperature of the previous record year 2005.

  13. JugendSCHUTZ – ja, aber wer und wie? Von Gesetzen, Werten und Ängsten

    Directory of Open Access Journals (Sweden)

    Christian Berger

    2010-11-01

    Full Text Available Wer die Freiheit aufgibt, um Sicherheit zu gewinnen, wird am Ende beides verlieren. (Benjamin Franklin Überlegungen zum Diskurs in der BRD über die Novellierung des Jugendmedienschutz-Staatsvertrages. In unserem Nachbarstaat, der Bundesrepublik Deutschland, wird derzeit heftigst über die Novellierung des Staatsvertrages über den Schutz der Menschenwürde und den Jugendschutz in Rundfunk und Telemedien (kurz: Jugendmedienschutz-Staatsvertrag oder JMStV diskutiert. Die Rechtslage in Deutschland ist etwas anders als in Österreich. Ein Staatsvertrag ist ein Gesetz, das von allen Bundesländern in gleicher Form beschlossen werden muss, damit es bundesweite Geltung erhält.

  14. Multi-fuctional heat recovery systems. Really energy saving; Multifunktionale Waermerueckgewinnung. Wie man wirklich Energie spart

    Energy Technology Data Exchange (ETDEWEB)

    Schilling, Heinz [SEW Systemtechnik fuer Energierecycling und Waermeflussbegrenzung GmbH, Kempen (Germany)

    2009-10-15

    An energy saving leeds only to a correct optimized operation, if a demand-oriented total design is integrated into the building. In special for air-conditioning energy saving measures are presented and essential criteria named, to save in fact effectively, efficiently, reliably and profitably energy and enhance simultanously user quality. (orig./GL)

  15. Huisartsenzorg in cijfers: het E-consult: hoe vaak en met wie?

    NARCIS (Netherlands)

    Verheij, R.; Ton, C.; Tates, K.

    2008-01-01

    Een consult per e-mail kan een normaal bezoek aan de huisarts vervangen. In 2006 werden op bijna twee miljoen consulten binnen de praktijken van het Landelijk Informatie Netwerk Huisartsenzorg (LINH) 1159 e-mailconsulten gedeclareerd. In ongeveer 30% van de 100 LINH-praktijken is in 2006 minstens éé

  16. Huisartsenzorg in cijfers: het E-consult: hoe vaak en met wie?

    OpenAIRE

    2008-01-01

    Een consult per e-mail kan een normaal bezoek aan de huisarts vervangen. In 2006 werden op bijna twee miljoen consulten binnen de praktijken van het Landelijk Informatie Netwerk Huisartsenzorg (LINH) 1159 e-mailconsulten gedeclareerd. In ongeveer 30% van de 100 LINH-praktijken is in 2006 minstens één e-mailconsult gedeclareerd. Vooral bij de gezondheidscentra. In de meeste praktijken bleef het beperkt tot minder dan 25 e-consulten per jaar. Drie praktijken waren al bijna goed voor de helft va...

  17. E-mobility. How does the future sound?; E-Mobilitaet. Wie klingt die Zukunft?

    Energy Technology Data Exchange (ETDEWEB)

    Pischinger, Stefan; Wolff, Klaus; Eisele, Georg; Genender, Peter [FEV Motorentechnik GmbH, Aachen (Germany); Schuermann, Gregor [RWTH Aachen Univ. (Germany). VKA Lehrstuhl fuer Verbrennungskraftmaschinen

    2011-07-01

    The interior noise of electric vehicles is analyzed by the example of FEV's Liiondrive and compared to the corresponding series vehicle Fiat 500. Beside objective analysis audio lab panel tests including virtual sound modifications were performed for subjective evaluation. Due to the missing combustion engine noise the Liiondrive is up to 12 dB(A) quieter than the ICE-vehicle. The pleasantness impression can be further improved by reducing the high frequency noise shares from gear toothing and electromagnetic orders. The low load dependency leads to a rather undynamic impression, which can be improved by a load dependent admix of low frequency E-motor orders. Due to the negative side effect on pleasantness this fits for electric vehicles in the sporty market segment. The limited driving range of an electric vehicle can be overcome by the installation of a range extender (RE), a combination of ICE-engine and generator. The noise contribution of this module should be kept below the vehicle speed dependent original interior noise of the electric vehicle by selection of a low noise engine concept, like e.g. Wankel engine, combined with a suitable mounting system and engine encapsulation. The operating strategy of the range extender module provides an additional degree of freedom for optimizing NVH. A noticeable range extender operation below 30 km/h should be avoided and a vehicle speed dependent range extender speed above 30 km/h makes optimal use of masking effects from road and wind noise and contributes to a good dynamic impression. (orig.)

  18. Van wie zegt de profeet dit? Een een-voudige vraag en een meervoudig antwoord

    Directory of Open Access Journals (Sweden)

    Rein Bos

    2005-10-01

    Full Text Available Of whom does the prophet say this? A single question and a multifaceted answerTheologians seeking to preach Old Testament texts meaningful way in Christian congregations face a great challenge. On the one hand, very little has been written in homiletical textbooks about hermeneutical problems facing those who wish to read the Old Testament from the perspective of Christ’s life and death. On the other hand, advances in biblical criticism seemed to have made any such attempt problematic to begin with. In this article, the author attempts to provide a practical-theological contribution to this hermeneutical challenge by reconsidering the heuristic value of mediaeval fourfold interpretation of scriptural passages. By focussing on the servant song of deutero-Isaiah (53 in light of its reinterpretation in Acts 8, this paper aims to provide some suggestions on how Christological interpretation of the Old Testament can be done in a way that takes the original context seriously and is able to read the text from a Christian perspective without the one reading infringing on the other.

  19. De tandem van Medvedev en Poetin: wie heeft de macht in Rusland?

    NARCIS (Netherlands)

    Jansen, M.

    2009-01-01

    Marc Jansen addresses the question who is the real leader in Russia at the moment. In May 2008, Vladimir Putin was succeeded as Russian President by his protégé Dmitrii Medvedev, but he stayed on as Prime Minister. A so-called 'tandem' was created, with Putin as the unchallenged No. 1. Whereas Medve

  20. Tagungsbericht: Gestirnte Kuppeln. Wie KulturwissenschaftlerInnen den Weltinnenraum des Planetariums erforschen

    Directory of Open Access Journals (Sweden)

    Florian Sprenger

    2013-09-01

    Full Text Available Florian Sprenger berichtet für die MEDIENIMPULSE von einer überaus spannenden Berliner Tagung, bei welcher der Weltinnenraum des Planetariums zwischen scientific visualization und event eingehend zum Gegenstand wurde. Was steht in den Sternen?

  1. How does an investor of biofuel projects decide?; Wie entscheidet ein Investor von Biotreibstoff-Projekten?

    Energy Technology Data Exchange (ETDEWEB)

    Hildebrand, K. [Premicon AG, Muenchen (Germany)

    2007-07-01

    Bioenergy and biofuels are a growth market in the Federal Republic of Germany in the next years as well as a young market. At this time, this market does not generate any profit so that any investments into this market can be realized. In order to finance the growth of this market, foreign capital is required. Under this aspect, within the scope of the 3rd symposium ''Processes and materials for energy technologies'', held between 20th and 21st June, 2007, at the ATZ Entwicklungszentrum in Sulzbach-Rosenberg (Federal Republic of Germany), the author reports on investment possibilities in biofuel projects such as closed investment funds, capital market, shares, open real estates and economic rents. The advantages of closed investment funds are: (a) long tradition and familiar form of investment; (b) increased corporate commitment. A long-term expectation into the market decides about an investment into closed funds; (c) There exists a great amount of issuing houses with long-term experiences; (d) Due to the omission of the medium funds and the wind-powered devices the market presently has a substantial volume. Apart from the fundamental considerations to investments also the basic data of the biofuel project are crucial: quality of the location, net yield and tax benefits.

  2. Wie is hier onredelijk!? Een analyse van de maatschappelijke dynamiek rondom de HPV-vaccinatiecampagne

    NARCIS (Netherlands)

    Meijer, A.; Lips, P.; Dijstelbloem, H.

    2014-01-01

    This article presents an analysis of the introduction of the HPV vaccine into the National Vaccination Program in the Netherlands. This introduction resulted in public debate and resistance and eventually a low turn-out (45% while 85% was expected). The question is what we can learn from this specif

  3. Japans Hauptstadt in Deutschland - - wie Düsseldorf sich zum wichtigsten Ziel japanischer Investitionen machte

    Science.gov (United States)

    Jäger, Kristina

    2017-02-01

    Local actors, such as municipal authorities, can promote and shape the settlement of foreign companies in a particular manner. In many instances, actions of economic promotion can lead to several conflicts; firstly between foreign investors and their national representatives, and secondly with (supra)regional industries or business circles. Here, flexible and long-term settlement projects, the permission of migrant infrastructure, and moreover, their high-profile marketing may attract foreign companies and long-term investors.

  4. How does a Swiss citizen want to heat his home?; Wie will der Schweizer heizen ?

    Energy Technology Data Exchange (ETDEWEB)

    Stadelmann, M

    2003-07-01

    This short article presents a summary of the results of representative market research carried out on which forms of energy are the most favoured in Switzerland. Solar energy is the form of energy at the top of the list of the most desired forms of energy for providing hot water. Natural gas is the energy carrier most favoured for space-heating, having for the first time overtaken solar energy in this sector. The article also quotes further findings of the survey, including the roles played by air pollution and other factors that influence energy desires. Also, the survey studied the sources used by the Swiss to obtain information on the subject of energy supply when building, buying or refurbishing their homes. The results are presented in the form of diagrams.

  5. Personal Branding von Musikern: Wie man im Musikgeschäft zu einer starken Marke wird

    OpenAIRE

    Schmidt, Holger J.; Horländer, Lisa

    2011-01-01

    In Zeiten einer hohen Reizüberflutung konkurrieren nicht nur Unternehmen sowie deren Produkte und Dienstleistungen um die Aufmerksamkeit ihrer Zielgruppen. Auch Personen des öffentlichen Lebens, z.B. Politiker, Künstler oder Wissenschaftler, stehen im Wettbewerb zueinander. Eine Möglichkeit, sich als Person dauerhaft von Anderen abzugrenzen und langfristig erfolgreich zu sein, bietet der Ansatz des Personal Branding. Unter dem Begriff Personal Branding, welches mitunter auch als Human Brandin...

  6. "Niedrigdosisabhängigkeit" von Benzodiazepinen - eine Sucht wie jede andere?

    OpenAIRE

    2008-01-01

    'Während das Konzept der Benzodiazepinabhängigkeit (BZDA) zunächst naturwissenschaftlich fundiert wirkt und sich auf neurobiologische Modelle stützen kann, so zeigt eine historische Rekonstruktion, dass die Psychiatrie sich eher an einem Konstruktionsprozess beteiligt, der nur partiell den Kriterien der evidenzbasierten Medizin folgt und eher Tendenzen eines gesamtgesellschaftlichen Diskurses aufgreift. In der Perspektive der evidenzbasierten Medizin gibt es durchaus Indikationen, die unter b...

  7. Wie krank ist die Vorhaut? Der Lichen sclerosus: Eine wichtige Entität im Kindesalter

    Directory of Open Access Journals (Sweden)

    Ebert A-K

    2011-01-01

    Full Text Available Einleitung: Das mögliche Behandlungsspektrum nicht retrahierbarer Vorhäute (= Phimose reicht von der topischen Kortisontherapie über vorhauterhaltende Erweiterungsplastiken bis zur radikalen Zirkumzision. Dabei weist die Mehrzahl der klinisch eindeutigen Phimosen eine normale Vorhauthistologie auf. Jedoch kann auch im Kindesalter ein Lichen sclerosus (LS in bis zu 20 % der Zirkumzisionen nachgewiesen werden. Methoden: Zwischen 08/ 2005 und 08/2007 wurden in der Kinderurologischen Klinik Regensburg 222 konsekutive Vorhautbiopsien histologisch untersucht. 69,8 % hatten eine normale Vorhauthistologie, 11,3 % einen LS, 8,1 % einen initialen Lichen und je 3,6 % eine unspezifische Entzündung, eine narbige Fibrose oder eine atopische Dermatitis. Allen 25 Patienten mit klassischem LS wurde eine Nachbehandlung mit topischem Tacrolimus im Sinne einer Anwendungsbeobachtung als „Off-label use“ angeboten. 20 Patienten applizierten postoperativ topisch Tacrolimus-0,1-%-Salbe für 3 Wochen und wurden median 13 Monate nachbeobachtet. 18 Patienten mit einem initialen Lichen wurden im gleichen Zeitraum ohne Therapie nachbeobachtet. Ergebnisse: Die topische Tacrolimus-Applikation wurde nebenwirkungsfrei vertragen, wobei 18 Patienten rezidivfrei blieben. Zwei Glans-Rezidive heilten erneut topisch mit Tacrolimus-0,1-%-Salbe aus. Keiner der Patienten mit einer lichenoiden Frühform erlitt ein Rezidiv oder einen Progress. Schlussfolgerungen: Der LS stellt eine schwer zu charakterisierende Erkrankung der Vorhaut, Glans und anterioren Urethra dar. Da es jedoch keine prädiktiven Faktoren für einen Progress oder ein mögliches Rezidiv des LS gibt, empfiehlt sich eine topische antientzündliche Nachbehandlung bei histologischer Diagnosestellung des LS unabhängig von der Lokalisation. Die anschließende Nachbeobachtung sollte engmaschig sein, um Rezidive frühzeitig erkennen und therapieren zu können.

  8. Creating Shared Value - Wie Porter den Kapitalismus neu erfinden möchte

    OpenAIRE

    Strathoff, Pepe

    2013-01-01

    Der Artikel beinhaltet einen kurzen Überblick und eine kritische Würdigung zum Creating Shared Value-Konzept von Michael Porter und Mark Kramer. Das von Michael Porter und Mark Kramer entwickelte Shared Value-Konzept verspricht eine neue Welle von Wachstum und Innovation. Und für das Jahr 2013 sieht der Economist einen Top 10-Trend in der Neuausrichtung von Unternehmen «from profit to purpose». Doch was steht hinter dem Konzept? Ein Überblick.

  9. Dicty_cDB: Contig-U03803-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available CH260-90L04_SP6 CHORI-260 Meleagris gallopavo gen... 46 2.5 1 ( ES222208 ) MpGVN_ag3_A19 Myzus persicae, li...ne G006, PLRV fre... 46 2.5 1 ( DW013102 ) w18o20_M13F Myzus persicae, tobacco lineage, whol... 46 2.5 1 ( C...( ES219391 ) MpGnorm_ag3_H23 Myzus persicae, tobacco lineage, ... 40 4.5 2 ( AM48...4.9 2 ( ES220416 ) MpGnorm_ag6_I23 Myzus persicae, tobacco lineage, ... 40 5.1 2 ( ES222123 ) MpGVN_ag2_M12 Myzus persicae...-UI.r1 Ceratitis capitata emb... 42 5.9 2 ( ES451001 ) 25971 Myzus persicae 2001-12 (red), Fenton Myzus ...

  10. Dicty_cDB: Contig-U15493-1 [Dicty_cDB

    Lifescience Database Archive (English)

    Full Text Available 41 7 ( FG076080 ) UI-FF-IF0-abn-h-19-0-UI.r1 Ceratitis capitata emb... 68 7e-41 7 ( EE009975 ) ROE00005661 Rhizopus oryzae Company...es ratti PA female naive SL... 88 4e-38 3 ( EE008512 ) ROE00004371 Rhizopus oryzae Company Rhizopus oryz... ..... 76 6e-36 5 ( EE007004 ) ROE00004237 Rhizopus oryzae Company Rhizopus oryz... 52 1e-35 6 ( DY886601 ) Cele...1525 ) Lysiphlebus testaceipes ribosomal protein L8 (RpL... 88 2e-34 5 ( EE008341 ) ROE00002957 Rhizopus oryzae Company... 4 ( EE004477 ) ROE00008423 Rhizopus oryzae Company Rhizopus oryz... 52 2e-32 6 ( EE004269 ) ROE00008327 Rhizopus oryzae Company

  11. Masters change, slaves remain.

    Science.gov (United States)

    Graham, Patricia; Penn, Jill K M; Schedl, Paul

    2003-01-01

    Sex determination offers an opportunity to address many classic questions of developmental biology. In addition, because sex determination evolves rapidly, it offers an opportunity to investigate the evolution of genetic hierarchies. Sex determination in Drosophila melanogaster is controlled by the master regulatory gene, Sex lethal (Sxl). DmSxl controls the alternative splicing of a downstream gene, transformer (tra), which acts with tra2 to control alternative splicing of doublesex (dsx). DmSxl also controls its own splicing, creating an autoregulatory feedback loop that ensures expression of Sxl in females, but not males. A recent paper has shown that in the dipteran Ceratitis capitata later (downstream) steps in the regulatory hierarchy are conserved, while earlier (upstream) steps are not. Cctra is regulated by alternative splicing and apparently controls the alternative splicing of Ccdsx. However, Cctra is not regulated by CcSxl. Instead it appears to autoregulate in a manner similar to the autoregulation seen with DmSxl.

  12. Effect of X-ray irradiation on fruit quality of clementine mandarin cv. 'Clemenules'

    Energy Technology Data Exchange (ETDEWEB)

    Alonso, Miquel [Centre de Technologia Postcollita, Institut Valencia d' Investigacions Agraries (IVIA), Ctra. de Montcada a Naquera, km. 4.5, E-46113 Montcada, Valencia (Spain); Palou, Lluis [Centre de Technologia Postcollita, Institut Valencia d' Investigacions Agraries (IVIA), Ctra. de Montcada a Naquera, km. 4.5, E-46113 Montcada, Valencia (Spain)]. E-mail: lluis.palou@ivia.es; Angel del Rio, Miguel [Centre de Technologia Postcollita, Institut Valencia d' Investigacions Agraries (IVIA), Ctra. de Montcada a Naquera, km. 4.5, E-46113 Montcada, Valencia (Spain); Jacas, Josep-Anton [Departament de Ciencies Experimentals, Universitat Jaume I (UJI), Campus del Riu Sec, E-12071 Castello de la Plana (Spain)

    2007-10-15

    The effects of a potential quarantine treatment consisting of exposure to X-ray irradiation against the Mediterranean fruit fly Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) on 'Clemenules' mandarin quality are presented and compared with those from the standard cold temperature quarantine treatment. X-ray irradiation doses of 0.195 and 0.395 kGy had no detrimental effects on fruit quality (rind color, firmness, juice yield, maturity index, internal volatiles, deterioration index and sensory evaluation). These results therefore indicate that X-ray irradiation is a harmless and highly effective quarantine technique for clementine mandarin and this technique could be as useful as the current cold treatment for 'Clemenules' mandarins.

  13. Essential oils composition of two Sicilian cultivars of Opuntia ficus-indica (L.) Mill. (Cactaceae) fruits (prickly pear).

    Science.gov (United States)

    Zito, Pietro; Sajeva, Maurizio; Bruno, Maurizio; Rosselli, Sergio; Maggio, Antonella; Senatore, Felice

    2013-01-01

    The essential oils composition of the skin, pulp and seeds from fruits of two Sicilian cultivars of Opuntia ficus-indica (cv. Sanguigna and cv. Surfarina) has been obtained by hydrodistillation and the possible antioxidant, antimicrobial and semiochemical roles have been investigated comparing the data with those reported in the literature. The presence of antioxidants and antimicrobials found in this study increases the spectrum of compounds that have beneficial properties in O. ficus-indica. In addition, several compounds identified in this study have been reported to influence the behaviour of Ceratitis capitata, a phytophagous pest which causes severe damages to several crops including O. ficus-indica and the kairomonal activity of the odour of the fruits seems provided by a blend of compounds found in the various matrices analysed.

  14. Effect of X-ray irradiation on fruit quality of clementine mandarin cv. ‘Clemenules’

    Science.gov (United States)

    Alonso, Miquel; Palou, Lluís; Ángel del Río, Miguel; Jacas, Josep-Anton

    2007-10-01

    The effects of a potential quarantine treatment consisting of exposure to X-ray irradiation against the Mediterranean fruit fly Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) on 'Clemenules' mandarin quality are presented and compared with those from the standard cold temperature quarantine treatment. X-ray irradiation doses of 0.195 and 0.395 kGy had no detrimental effects on fruit quality (rind color, firmness, juice yield, maturity index, internal volatiles, deterioration index and sensory evaluation). These results therefore indicate that X-ray irradiation is a harmless and highly effective quarantine technique for clementine mandarin and this technique could be as useful as the current cold treatment for 'Clemenules' mandarins.

  15. Overview of prohibited and permitted plant regulatory listing systems

    Science.gov (United States)

    Westbrooks, Randy G.; Tasker, Alan V.

    2011-01-01

    Pest risk analysis is a process that evaluates the risks involved with a proposed species to help determine whether it should be permitted or denied entry into a country, and how the risks could be managed if it is imported. The prohibited listing approach was developed in the late 1800s and early 1900s in response to outbreaks of plant and animals pests such as foot and mouth disease of livestock, Mediterranean fruitfly (Ceratitis capitata Wiedemann), and Gypsy moth (Lymantria dispar L.). Under this approach, selected species of concern are evaluated to determine if they should be regulated for entry. Under the permitted listing approach that was first used on a national level in Australia in the 1990s, all species that are proposed for introduction are assessed to determine if they should be regulated.

  16. 福州实蝇监测初报%A preliminary report on the monitoring of Bactrocera in Fuzhou

    Institute of Scientific and Technical Information of China (English)

    林惠敏; 陈艳; 范青海

    2006-01-01

    自2005年5月29日至2006年5月28日在福州金山福建农林大学校园内进行了实蝇监测.采用甲基丁香酚(Me)、诱蝇酮(Cue)和地中海实蝇诱芯(T),诱集到橘小实蝇[Bactrocera(Bactrocera)dorsalis]、瓜实蝇[B.(Zeugodacus)cucurbitae]、南瓜实蝇[B.(Zeugodacus)tau]和具条实蝇(B.scutellata)4种实蝇,但未诱到地中海实蝇(Ceratitis capitata).其中,橘小实蝇诱集量最大,8月中下旬达到高峰.本研究为掌握福州地区实蝇发生动态提供了基础资料.

  17. 柳州市2000~2008年检疫性实蝇监测调查报告

    Institute of Scientific and Technical Information of China (English)

    谢晓雁; 贝荣良; 殷积奎

    2010-01-01

    2000~2008 年在柳州市以及各县区范围内建立检疫性实蝇监测体系,对柳州市及周边县区实蝇的发生进行监测调查,通过对调查结果的统计分析,确定柳州市检疫性实蝇发生的优势种和高发期,监测调查结果表明:柳州市未发现地中海实蝇Ceratitis capitata(Widedmann)、墨西哥实蝇Anastrepha ludens、加勒比实蝇A.suspensa 等我国过去没有报道的实蝇种类.

  18. A Computer Model of Insect Traps in a Landscape

    Science.gov (United States)

    Manoukis, Nicholas C.; Hall, Brian; Geib, Scott M.

    2014-11-01

    Attractant-based trap networks are important elements of invasive insect detection, pest control, and basic research programs. We present a landscape-level, spatially explicit model of trap networks, focused on detection, that incorporates variable attractiveness of traps and a movement model for insect dispersion. We describe the model and validate its behavior using field trap data on networks targeting two species, Ceratitis capitata and Anoplophora glabripennis. Our model will assist efforts to optimize trap networks by 1) introducing an accessible and realistic mathematical characterization of the operation of a single trap that lends itself easily to parametrization via field experiments and 2) allowing direct quantification and comparison of sensitivity between trap networks. Results from the two case studies indicate that the relationship between number of traps and their spatial distribution and capture probability under the model is qualitatively dependent on the attractiveness of the traps, a result with important practical consequences.

  19. Identification of Fruit Fly Low Instar Larvae in Imported Red Pepper%进境辣椒中低龄辣椒实蝇幼虫的鉴定

    Institute of Scientific and Technical Information of China (English)

    孙涛; 陆丽华; 滕少娜; 邓朝晖; 赵野; 刘宏; 孔德英

    2013-01-01

    自菲律宾进境旅客携带的小红辣椒中截获1头低龄实蝇幼虫.分别用辣椒实蝇(Bactrocera latifrons)、橘小实蝇(B.dorsalis)和地中海实蝇(Ceratitis capitata)的特异性引物lati1/lati2、PF-1/PF-2和CCCA-COL-L/CCCA-COL-R对样品总DNA进行PCR扩增和测序后,与Genbank中已知序列比对,结果表明该实蝇与Genbank中辣椒实蝇(FJ903498.1)的同源性达99%.结合形态学特征,确认截获的样本为辣椒实蝇(Bactrocera latifrons).

  20. Temporal Diversity and Abundance Patterns of Parasitoids of Fruit-Infesting Tephritidae (Diptera) in the Argentinean Yungas: Implications for Biological Control.

    Science.gov (United States)

    Schliserman, Pablo; Aluja, Martin; Rull, Juan; Ovruski, Sergio M

    2016-10-01

    A 4-yr study was done to analyze seasonal patterns underlying host plant-fruit fly-parasitoid interactions in a secondary forest in the Argentinean Yunga and its importance for the implementation of conservation and augmentative biological control. Larval-pupal hymenopteran parasitoids associated with all host plants and fruit fly species were identified and the seasonal occurrence of fruit, infestation levels, parasitism percentage, and relative parasitoid abundance were determined. Three fruit fly species in two genera were found in association with surveyed plants, two of which (Ceratitis capitata (Wiedemann) and Anastrepha fraterculus (Wiedemann)) are of major economic importance. Infestation levels were strongly influenced by environmental factors and peak fruit availability. Five fruit fly parasitoid species were recovered from fly pupae, four braconid species, and one figitid. Time windows for fruit fly population growth were pinpointed. Based on results, the present analysis proposes an effective fruit fly biological control strategy tailored for the northwestern Argentinean citrus-producing area.

  1. Fruit flies (Diptera, Tephritidae) and their associations with native host plants in a remnant area of the highly endangered Atlantic Rain Forest in the State of Espírito Santo, Brazil.

    Science.gov (United States)

    Uramoto, K; Martins, D S; Zucchi, R A

    2008-10-01

    The results presented in this paper refer to a host survey, lasting approximately three and a half years (February 2003-July 2006), undertaken in the Vale do Rio Doce Natural Reserve, a remnant area of the highly endangered Atlantic Rain Forest located in Linhares County, State of Espírito Santo, Brazil. A total of 330 fruit samples were collected from native plants, representing 248 species and 51 plant families. Myrtaceae was the most diverse family with 54 sampled species. Twenty-eight plant species, from ten families, are hosts of ten Anastrepha species and of Ceratitis capitata (Wiedemann). Among 33 associations between host plants and fruit flies, 20 constitute new records, including the records of host plants for A. fumipennis Lima and A. nascimentoi Zucchi. The findings were discussed in the light of their implications for rain forest conservation efforts and the study of evolutionary relationships between fruit flies and their hosts.

  2. Moscas-das-frutas em pomares de pessegueiro e maracujazeiro, no Município de Iraceminha, Santa Catarina, Brasil Fruit flies in peach and passion fruit orchards in Iraceminha, Santa Catarina, Brazil

    Directory of Open Access Journals (Sweden)

    Sheila Alberti

    2009-08-01

    Full Text Available Este estudo teve o objetivo de caracterizar a comunidade de moscas-das-frutas de ocorrência em pomares de pessegueiro e maracujazeiro, no Município de Iraceminha, Santa Catarina (SC, por meio da análise faunística. Os adultos de moscas-das-frutas foram capturados semanalmente, em armadilhas McPhail, contendo glicose invertida a 10%, no período de abril de 2006 a março de 2007. Na caracterização, foram calculados os índices de abundância, constância, dominância, frequência e diversidade. Foram coletados 697 adultos de moscas-das-frutas. Anastrepha grandis e Anastrepha fraterculus foram as espécies mais abundantes, frequentes, constantes e dominantes nos pomares estudados, predominando sobre as outras espécies de moscas-das-frutas. Ceratitis capitata foi caracterizada como dispersa e pouco frequente. O maior índice de diversidade encontrado foi de 1,99 no pomar de maracujazeiro.This research had the objective to characterize the community of fruit flies in peach and passion fruit orchards in Iraceminha, Santa Catarina, Brazil, through faunistic analyses. The adults fruit flies were weekly captured in McPhail traps with 10% inverted glucose, from April 2006 to March 2007. In the characterization, the indices for abundance, constancy, dominance, frequency and diversity were calculated. A total of 697 adults fruit flies were collected. Anastrepha grandis and Anastrepha fraterculus species were the most abundant, frequent, constant and dominant in orchards studied, predominating upon other fruit flies species. Ceratitis capitata was dispersed and not very frequent. The biggest index of diversity of 1.99 was in passion fruit orchard.

  3. Adult population dynamics of the bolivian fruit flies Anastrepha sp. (Diptera: Tephritidae at Municipality Coroico, Department of The La Paz, Bolivia

    Directory of Open Access Journals (Sweden)

    Gonzáles Manuel

    2011-08-01

    Full Text Available The investigation was carried out in Paco (1603 msnm communities, it Marca (1511 msnm and Capellania (1720 msnm, of the Municipality of Coroico, department of La Paz, Bolivia. In orchards frutícolas semicomerciales, they settled 15 traps distributed McPhail in a similar way among areas, five for community, sampling" "points. The censuses were carried out with an interval of 15 days, they were identified and they quantified the mature flies of the fruit. For the captures of the individuals, they settled the traps McPhail, using the attractive (Buminal one and as conserving borax. The traps were distributed in representative parcels, having as main cultivations, orange, mandarin, grapefruit, guava and avocado. The identification taxonómica of the captured species was carried out in the laboratory of the National Program of Control of Flies of the fruit (PROMOSCA, clerk of the National Service of Agricultural Sanity and Alimentary (SENASAG Inocuidad. 1210 mature flies of the fruit were captures, those that grouped for species, sex, capture dates and community, corresponding to the seven carried out censuses. The species of Anastrepha fraterculus (Wiedeman were identified, Anastrepha striata Schiner, Anastrepha serpentine (Wiedeman, Anastrepha sp, Ceratitis capitata (Wiedemann, Blepharoneura sp Loew, Hexaresta sp Hering, Hexachaeta sp Loew, Tomoplagia sp Coquillett, Tetreuaresta sp Hendel, being that of more presence Anastrepha fraterculus (Wiedeman with 818 and Ceratitis capitata (Wiedemann, with 354. The temperature and presence of spices put up frutícolas of flies of the fruit in maturation state explain the observed fluctuations.

  4. Environ: E00691 [KEGG MEDICUS

    Lifescience Database Archive (English)

    Full Text Available E00691 Cyathula capitata root Crude drug Cyathula capitata, Cyathula [TAX:221765] Amaranthaceae (amaranth... family) Cyathula capitata root (dried) Crude drugs [BR:br08305] Dicot plants: others Amaranthaceae (amaranth family) E00691 Cyathula capitata root ...

  5. Hole diameters in pet bottles used for fruit fly capture

    Directory of Open Access Journals (Sweden)

    Maurico Paulo Batistella Pasini

    2015-05-01

    Full Text Available Two experiments were conducted during the period from 31 January to 6 March 2012 in Santa Maria, Rio Grande do Sul State, Brazil to determine the efficiency of different hole diameters in PET trap bottles on pests in guava and persimmon orchards. In a randomised block design in a factorial scheme, we assessed the average number adults of Anastrepha fraterculus, Ceratitis capitata (Diptera: Tephitidae and Zaprionus indianus (Diptera: Drosophilidae infruits thatemerged in two situations (in the plant and on the soil; we also assessed the number of captured adults in trap bottlesunder two conditions, different hole diameters and different days after placement of the attractive solution. Smaller diameter sizescaptured more A. fraterculus, C. capitata and Z. indianusadults. The 1.0 cm diameter was the most efficient hole size in reducing the adult emergence of Tephritidae to Z. indianus, whereas the smallest diameter hole sizes, 0.6 and 0.8 cm, showed the highest efficiencies in controlling adult emergence in persimmon fruit and guava fruit.

  6. Mass-rearing of Mediterranean fruit fly using low-cost yeast products produced in Brazil

    Directory of Open Access Journals (Sweden)

    Alberto Moreira da Silva Neto

    2012-12-01

    Full Text Available Ceratitis capitata is one of the most important pests of fruits for exportation, and Sterile Insect Technique (SIT has been the most efficient and environmental friendly technique used to control fruit fly populations around the world. A key goal in achieving a successful SIT program is a mass rearing system producing high quality insects at low cost. Providing adults with an artificial diet containing hydrolysed protein has been the major obstacle for bio-production facilities in Brazil, because it is expensive and has to be imported. Two other commercial products, autolysed yeast (AY and yeast extract (YE, of domestic origin and low cost, were tested as substitutes of the imported hydrolyzed protein. To compare their efficiency we observed the female fecundity, adult survival and egg viability of flies raised on diets containing one of each of the different protein products. Flies reared on the domestic yeast products had equivalent or superior performance to the flies reared on imported protein. Both AY and YE can be a possible substitute for imported hydrolyzed protein for C. capitata mass-rearing, as they are cheaper and are readily available in the national market.

  7. Biological Control of Tephritid Fruit Flies in Argentina: Historical Review, Current Status, and Future Trends for Developing a Parasitoid Mass-Release Program

    Directory of Open Access Journals (Sweden)

    Sergio M. Ovruski

    2012-09-01

    Full Text Available In Argentina there are two tephritid fruit fly species of major economic and quarantine importance: the exotic Ceratitis capitata that originated from Southeast Africa and the native Anastrepha fraterculus. In recent years, the use of fruit fly parasitoids as biocontrol agents has received renewed attention. This increasing interest has recently led to the establishment of a program for the mass rearing of five million Diachasmimorpha longicaudata parasitoids per week in the BioPlanta San Juan facility, San Juan, Argentina. The first augmentative releases of D. longicaudata in Argentina are currently occurring on commercial fig crops in rural areas of San Juan as part of an integrated fruit fly management program on an area-wide basis. In this context, research is ongoing to assess the suitability of indigenous parasitoid species for successful mass rearing on larvae of either C. capitata or A. fraterculus. The purpose of this article is to provide a historical overview of the biological control of the fruit fly in Argentina, report on the strategies currently used in Argentina, present information on native parasitoids as potential biocontrol agents, and discuss the establishment of a long-term fruit fly biological control program, including augmentative and conservation modalities, in Argentina’s various fruit growing regions.

  8. Evidence for potential of managing some African fruit fly species (Diptera: Tephritidae) using the mango fruit fly host-marking pheromone.

    Science.gov (United States)

    Kachigamba, Donald L; Ekesi, Sunday; Ndungu, Mary W; Gitonga, Linus M; Teal, Peter E A; Torto, Baldwyn

    2012-12-01

    We investigated conspecific and heterospecific oviposition host discrimination among four economically important fruit fly pests of mango in Africa (Ceratitis capitata, Wiedemann; C. fasciventris, Bezzi; C. rosa, Karsch, and C. cosyra, Walker) with regard to host-marking behavior and fecal matter aqueous solutions. The objective of the study was to get insight into the potential of managing these pests using the host-marking technique. Observations were done on mango slices marked by the flies and treated with aqueous solutions of fecal matter of the flies, respectively. In both host-marking and fecal matter experiments, C. cosyra, which is the most destructive species of the four on mango, was exceptional. It only discriminated against hosts treated with its fecal matter but with lower sensitivity while C. capitata and C.fasciventris discriminated against hosts marked by it or treated with its fecal matter and with higher sensitivity. Our results provide evidence for potential of managing some of the major fruit fly species infesting mango in Africa using the host-marking pheromone of the mango fruit fly, C. cosyra.

  9. Incidence of fruit flies on coffee and citrus and quarantine treatment of citrus fruits by gamma radiation; Incidencia de moscas-das-frutas em cafe e citros e tratamento quarentenario de frutos citricos com radiacao gama

    Energy Technology Data Exchange (ETDEWEB)

    Raga, Adalton

    1996-12-31

    The objective of this study was to evaluate the fruit fly infestation on coffee and citrus, and also to determine gamma radiation doses for immature stages of Ceratitis capitata and Anastrepha fraterculus, in order to satisfy quarantine regulations. Coffee arabica varieties Icatu Vermelho, Catuai Amarelo, Mundo Novo and Sarchimor showed the highest infestation indices (pupa/berry): 0.53; 0,41; 0.33 and 0.36. respectively Icatu Vermelho and Catuai Vermelho showed the highest values of pupa/berry weight (0.49 and 0.39, respectively), and Robusta (Coffea canephora) presented the lowest index (0.01). The following fruit flies were found in coffee berries: C. capitata (76.6%) Anastrepha spp. (7.4%) and Lonchaeidae (17.0%). In area near coffee plantation, fruit fly infestation indices in sweet oranges were of 4.77 larvae/kg and 0.55 larva/fruit. The infestation indices for sweet orange, collected from five regions of the State of Sao Paulo ranged from 0.73 to 7.60 pupa/kg and 0.12 to 1.27 pupa/fruit. The same species of fruit flies were found in oranges. In the case of C. capitata eggs with 24-48 hours old, 20 Gy prevented completely adult emergence (artificial diet and orange). No emergence of adult occurred when C. capitata larvae of third instar were irradiated at 20 Gy in their rearing medium. But at 25 Gy, the number of adults was reduced by 54% and 97% from larval infestation in oranges and grapefruit, respectively. A dose of 30 Gy was required to prevent medfly emergence from third instar larvae in grapefruit. A dose of 15 Gy was required for third instar, to prevent adult emergence of A. fraterculus. No adult emerged from C third instar, to prevent adult emergence of A. fraterculus. No adult emerged from C capitata pre-pupa irradiated at 30 Gy. One medfly adult emerged from pupa (3-4 days after pupating) irradiated at 120 Gy. At the same dose, sixteen A. fraterculus adults emergency from irradiated pupa with 5-6 days old. (author) 85 refs., 2 figs., 13 tabs.

  10. Moscas frugívoras (Tephritidae e Lonchaeidae: ocorrência em pomares comerciais de tangerina (Citrus reticulata Blanco do município de Matinhas, Estado da Paraíba = Frugivorous flies (Tephritidae and Lonchaeidae: occurrence in commercials tangerine orchards (Citrus reticulata Blanco in Matinhas, state of Paraíba, Brazil

    Directory of Open Access Journals (Sweden)

    Edson Batista Lopes

    2008-12-01

    Full Text Available O município de Matinhas, Estado da Paraíba com aproximadamente 1,3 milhões de plantas de tangerina, é responsável por 90% da produção do Estado. A pesquisa foi conduzida em cinco regiões geográficas do município de Matinhas, onde foram coletados 20 frutos na copa e 20 sob a copa da planta, objetivando pesquisar a ocorrência de moscasfrugívoras e seus níveis de infestação em tangerina. Os resultados obtidos evidenciaram que a tangerina é infestada por Ceratitis capitata (Wiedmann,1824, Neosilba zadolicha (McAlpine e Steyskal e Neosilba glaberrima (Wiedmann,1830. O índice médio de infestação de C. capitata nas cinco regiões não ultrapassou 0,5 pupa fruto-1. A percentagem de emergência (P.E. de C. capitata em frutos coletados na planta e solo variou entre 14,0 a 54,0% de adultos fruto-1. Osíndices de infestação de N. zadolicha, em frutos coletados na planta e solo, variaram entre 0,4 a 4,3 pupários fruto-1. Os dados da percentagem de emergência (P.E. de N. zadolicha variaram entre 49,9 a 65,9% de adultos fruto-1, sendo considerada a espécie mais abundante, dominante e com uma alta taxa de sobrevivência. N. zadolicha foi considerada praga primária da tangerina nas condições de Matinhas. Este é, também, o primeiro relato das espécies N. zadolicha e N. glaberrima infestando tangerina na Paraíba.The municipality of Matinhas, with approximately 1.3 million tangerinetrees, is responsible for 90% of the tangerine production in the state of Paraíba. This research was carried out in five geographic regions of Matinhas, where 20 fruits on plant and 20 fruits in soil were collected, with the aim of searching for the frugivorous flies and their levels of infestation in tangerine. The results showed that tangerine is infested by frugivorous flies Ceratitis capitata (Wiedmann, 1824, Neosilba zadolicha (McAlpine e Steyskal and Neosilba glaberrima (Wiedmann, 1830. The mean infestation index of C. capitata in five regions did

  11. Horizontal Transmission of Metarhizium anisopliae in Fruit Flies and Effect of Fungal Infection on Egg Laying and Fertility

    Directory of Open Access Journals (Sweden)

    Nguya K. Maniania

    2013-05-01

    Full Text Available Fly-to-fly transmission of conidia of the entomopathogenic fungus Metarhizium anisopliae and the effect of fungal infection on the reproductive potential of females surviving infection were investigated in three fruit fly species, Ceratitis cosyra, C. fasciventris, and C. capitata. The number of conidia picked up by a single fruit fly was determined in C. cosyra. The initial uptake (Day 0 of conidia by a single fly was approx. 1.1 × 106 conidia after exposure to the treated substrate. However, the number of conidia dropped from 7.2 × 105 to 4.1 × 105 conidia after 2 and 8 h post-exposure, respectively. The number of conidia picked up by a single fungus-treated fly (“donor” varied between 3.8 × 105 and 1.0 × 106 in the three fruit fly species, resulting in 100% mortality 5–6 days post-exposure. When fungus-free flies of both sexes (“recipient” flies were allowed to mate with “donor” flies, the number of conidia picked up by a single fly varied between 1.0 × 105 and 2.5 × 105, resulting in a mortality of 83–100% in C. capitata, 72–85% in C. cosyra and 71–93% in C. fasciventris 10–15 days post-inoculation. There was an effect of fungal infection on female egg laying in the three species of fruit flies as control flies laid more eggs than fungus-treated females. The percentage reduction in fecundity in flies infected with M. anisopliae was 82, 73 and 37% in C. capitata, C. fasciventris and C. cosyra, respectively. The results are discussed with regard to application in autodissemination techniques.

  12. A comparative assessment of the response of three fruit fly species (Diptera: Tephritidae) to a spinosad-based bait: effect of ammonium acetate, female age, and protein hunger.

    Science.gov (United States)

    Piñero, J C; Mau, R F L; Vargas, R I

    2011-08-01

    Ammonia-releasing substances are known to play an important role in fruit fly (Diptera: Tephritidae) attraction to food sources, and this information has been exploited for the development of effective synthetic food-based lures and insecticidal baits. In field studies conducted in Hawaii, we examined the behavioural response of wild female oriental fruit fly (Bactrocera dorsalis (Hendel)), melon fly (B. cucurbitae (Coquillett)), and Mediterranean fruit fly (Ceratitis capitata (Wiedemann)) to spinosad-based GF-120 NF Naturalyte Fruit Fly Bait(©) formulated to contain either 0, 1 or 2% ammonium acetate. Use of visually-attractive yellow bait stations for bait application in the field allowed for proper comparisons among bait formulations. Field cage tests were also conducted to investigate, using a comparative behavioural approach, the effects of female age and protein starvation on the subsequent response of F1 generation B. cucurbitae and B. dorsalis to the same three bait formulations that were evaluated in the field. Our field results indicate a significant positive effect of the presence, regardless of amount, of AA in GF-120 for B. dorsalis and B. cucurbitae. For C. capitata, there was a significant positive linear relationship between the relative amounts of AA in bait and female response. GF-120 with no AA was significantly more attractive to female C. capitata, but not to female B. dorsalis or B. cucurbitae, than the control treatment. Our field cage results indicate that the effects of varying amounts of AA present in GF-120 can be modulated by the physiological stage of the female flies and that the response of female B. cucurbitae to GF-120 was consistently greater than that of B. dorsalis over the various ages and levels of protein starvation regimes evaluated. Results are discussed in light of their applications for effective fruit fly suppression.

  13. The thermodynamic stability and hydration enthalpy of strontium cerate doped by yttrium

    OpenAIRE

    Matskevich, N. I.; Wolf, Th.; Matskevich, M. Yu.

    2005-01-01

    The standard molar enthalpy of formation of SrY0.05Ce0.95O2.975 has been derived by combining the enthalpy of solution of this compound in 1 M HCl + 0.1 KI obtained by us and auxiliary literature data. The following value has been derived: DfH (SrY0.05Ce0.95O2.975, s, 298.15 K) = -1720.4 (3.4) kJ/mol. The obtained value has been used to obtain the formation enthalpy of SrY0.05Ce0.95O2.975 from the mixture of binary oxides (DoxH (298.15 K) = -45.9 (3.4) kJ/mol) and formation enthalpy of reacti...

  14. Role of CA-EDTA on the Synthesizing Process of Cerate-Zirconate Ceramics Electrolyte

    Directory of Open Access Journals (Sweden)

    Nur Athirah Abdullah

    2013-01-01

    Full Text Available The role of a combination between citric acid (CA and ethylenediaminetetra acetic acid (EDTA as chelating agents in preparation of BaCe0.54Zr0.36Y0.1O2.95 powder by a modified sol-gel method is reported. The precursor solutions were prepared from metal nitrate salts (M+, chelating agents (C, and ethylene glycol (EG at molar ratio of M+ : C : EG = 3 : 2 : 3. Chemical and phase transformation of samples during thermal decomposition were analyzed by thermogravimetric analysis (TGA and Fourier transform infrared (FTIR spectroscopy. TGA results show that the sample prepared by a combination of CA-EDTA exhibited the lowest thermal decomposition temperature, Ttd since there was no significant weight loss after 750°C. After calcined at 1100°C, the carbonates residue remained in the samples as proven by FTIR results. It was found that the used combination of CA-EDTA acts as a better combustion reagent to increase the reaction rate and influence the thermal decomposition behaviour compared to a single citric acid and EDTA, respectively. Apparently, calcination temperatures above 1100°C are needed to produce a pure perovskitic BaCe0.54Zr0.36Y0.1O2.95.

  15. A high-resolution neutron powder diffraction study of neodymium doping in barium cerate

    DEFF Research Database (Denmark)

    Knight, K.S.; Bonanos, N.

    1995-01-01

    in the thermal expansion properties are small in BaCe1-xNdxO3-x/2. The spontaneous strain decreases with increasing neodymium doping showing the deviation from an ideal cubic aristotype reduces with doping level. There is some evidence for saturation in the spontaneous strain at x>0.2.......High-resolution neutron powder diffraction data have been collected on 6 perovskites of composition BaCe1-xNdxO3-x/(2), with 0 less than or equal to x less than or equal to 0.2, in which structural phase transitions Pmcn-->P4/mbm at x=0.05, and P4/mbm-->Pm3m at x=0.1, were inferred from a recent...... Raman spectroscopy study. Our results contradict these conclusions; for 0 less than or equal to x less than or equal to 0.16, BaCe1-xNdxO3-x/2 is orthorhombic, space group Pmcn. At x=0.2, the powder data are consistent with either space group Pmcn or Cmcm, and could not be distinguished using structural...

  16. Low temperature preparation and fuel cell properties of rare earth doped barium cerate solid electrolytes

    Institute of Scientific and Technical Information of China (English)

    蒋凯; 何志奇; 孟建; 任玉芳; 苏锵

    1999-01-01

    The solid electrolytes, BaCe0.8 Ln0.2O2.9 (Ln: Gd, Sm, Eu), were prepared by the sol-gel method. XRD indicated that a pure orthorhombic phase was formed at 900℃. The synthesis temperature by the sol-gel method was about 600℃ lower than the high temperature solid phase reaction method, The electrical conductivity and impedance spectra were measured and the conduction mechanism was studied. The grain-boundary resistance of the solid electrolyte could be reduced or eliminated by the sol-gel method. The conductivity of BaCe0.8Gd0.2O2.9 is 7.87×10-2 S·cm-1 at 800℃. The open-circuit voltage of hydrogen-oxygen fuel cell using BaCe0.8 Gd0.2O2.9 as electrolyte was near to 1 V and its maximum power density was 30 mW·cm-2.

  17. Extraction, partial purification and characterization of acidic peroxidase from cabbage leaves (Brasicca olearacea var. capitata

    Directory of Open Access Journals (Sweden)

    Aniruddha Bhalchandra Pandit

    2012-08-01

    Full Text Available Normal 0 false false false EN-US X-NONE X-NONE MicrosoftInternetExplorer4 The present work deals with extraction of cabbage peroxidase (CP from fresh cabbage leaves and subsequent purification using ammonium sulphate (80% w/v precipitation. The peroxidase extraction has been carried out by screening two different cabbage and then different parameters like different buffer systems, strength of buffers, buffer volumes, grinding time and cabbage leaves weight ratio to buffer volumes were optimized. The purified peroxidase showed maximum activity at pH 5.5 and at temperature 55 °C. The enzyme action followed the Michelis–Menton kinetics and gave a Km of 0.7018 mg/ml for Guaiacol oxidation over different concentrations (0 – 10 mg/ml at pH 5.0 and Vmax was obtained as 0.6498 mg/min.ml. The molecular weight of the partially purified enzyme was found to be about 67,000 Daltons using SDS-PAGE and zymogram method. /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;}

  18. Histopathology of Brassica oleracea var. capitata subvar. alba infected with Heterodera cruciferae Franklin, 1945 (Tylenchida: Heteroderidae)

    Science.gov (United States)

    Because anatomical changes induced by the cabbage cyst nematode (Heterodera cruciferae) have been insufficiently characterized, here we describe these changes in the root tissues of white head cabbage varieties commonly grown in the Black Sea Region of Turkey, where cabbage-growing areas are heavily...

  19. Effects of intraspecific variation in white cabbage (Brassica oleracea var. capitata) on soil organisms

    NARCIS (Netherlands)

    Kabouw, P.; Van der Putten, W.H.; Van Dam, N.M.; Biere, A.

    2010-01-01

    Intraspecific variation in plants can affect soil organisms. However, little is known about whether the magnitude of the effect depends on the degree of interaction with the roots. We analyzed effects of plant intraspecific variation on root herbivores and other soil organisms that interact directly

  20. Effects of intraspecific variation in white cabbage (Brassica oleracea var. Capitata) on soil ogranisms

    NARCIS (Netherlands)

    Kabouw, P.; Putten, van der W.H.; Dam, van N.M.; Biere, A.

    2010-01-01

    Intraspecific variation in plants can affect soil organisms. However, little is known about whether the magnitude of the effect depends on the degree of interaction with the roots. We analyzed effects of plant intraspecific variation on root herbivores and other soil organisms that interact directly

  1. Plant traits associated with resistance to Thrips tabaci in cabbage (Brassica oleracea var capitata)

    NARCIS (Netherlands)

    Voorrips, R.E.; Steenhuis, M.M.; Tiemens-Hulscher, M.; Lammerts Van Bueren, E.

    2008-01-01

    Thrips tabaci is a major problem in the cultivation of cabbage for storage, as this pest causes symptoms that necessitate the removal of affected leaves from the product. Between cabbage varieties large differences in susceptibility occur. This study aimed to identify plant traits associated with th

  2. Off with oil - but how? Energy supply, sustainability, climate change, resource shortage - how do we manage this?; Weg vom Oel - aber wie? Energieversorgung, Nachhaltigkeit, Klimawandel, Ressourcenknappheit - Wie ist das zu schaffen?

    Energy Technology Data Exchange (ETDEWEB)

    Keppler, Erhard

    2008-07-01

    The author summarizes critically present national and European energy policy with a view of energy political need for action in 21st century. For this reason presented are realized discussed possibilities to generate energy. Besides specific principles of energy gaining all potentials are estimated and possible contribution on energy supply discussed. All fossil fuels are covered, as all important regenerative methods for energy generation, incl. nuclear power, as energy storage, energy transport, waste processing and possibilities for energy saving. The last chapter discusses white hopes of energy production, below also nuclear fusion. (orig./GL) [German] Der Autor zieht eine kritische Bilanz der gegenwaertigen nationalen und europaeischen Energiepolitik - mit Blick auf energiepolitischen Handlungsbedarf im 21. Jahrhundert. Hierbei werden die gegenwaertig diskutierten Moeglichkeiten, Energie zu erzeugen, dargestellt. Neben den spezifischen Prinzipien der Energiegewinnung werden deren Potenziale abgeschaetzt und die moeglichen Beitraege zur Energieversorgung eroertert. Alle fossilen Energietraeger werden behandelt, ebenso alle wichtigen regenerativen Energieerzeugungsmethoden, Kernenergie eingeschlossen, sowie Energiespeicherung, Energietransport, Abfallverwertung und Energieeinsparmoeglichkeiten. Das letzte Kapitel diskutiert die ''Hoffnungstraeger'' kuenftiger Energieerzeugungsmoeglichkeiten, darunter auch die Fusion. (orig.)

  3. The large questions after Chernobyl. What are Becquerels and what are the hazards?; Die grosse Frage nach Tschernobyl. Was sind und wie misst man Bequerels, und wie gefaehrlich sind sie?

    Energy Technology Data Exchange (ETDEWEB)

    Maushart, Rupprecht

    2016-05-01

    In consequence of Chernobyl private persons and public officers wanted or had to measure radioactivity without any knowledge on the issue. Experiences form this situation are summarized and discussed with respect to the production of appropriate measuring instruments and reasonable descriptions.

  4. Mythos Flexibilisierung? Wie instabil sind Berufsbiografien wirklich und als wie instabil werden sie wahrgenommen? = Is occupational flexibilization a myth? How stable have working lives been and as how stable are they being perceived?

    NARCIS (Netherlands)

    Mayer, K.U.; Grunow, D.; Nitsche, N.

    2010-01-01

    In this article, we address the current debate of increasing work life flexibility in (West-) Germany. In order to shed some light on the contradiction between a widely accepted decrease in work life stability and empirical findings that do not confirm such a decline, we contrast "objective" evidenc

  5. Estudo histológico e histoquímico da glândula de Duvernoy de Clelia plumbea (Wied (Serpentes, Colubridae, Xenodontinae Histological and histochemical study of Duvernoy's gland from Clelia plumbea (Wied (Serpentes, Colubridae, Xenodontinae

    Directory of Open Access Journals (Sweden)

    Márcia Ferret Renner

    2000-09-01

    Full Text Available There are report two human envenenomations for species from Clelia Fitzinger, 1826 (opisthoglyph snake. The patients exhibited symptoms similar from bothropic accident. Then this work have with objective, the hystological and histochemical study of Duvernoy's gland from Clelia plumbea. For the hystological observation of the Duvernoy's gland were using, with paraffin's cutting edge, two methods: Hematoxylin + Eosin and Toluidine's Blue. With historesine's cutting edge were using three methods: Metilen's Blue, Toluidine's Blue + Floxine and Toluidine's Blue. For the histochemical detection were using six techniques, with paraffin's cutting edge: PAS, PAS + Alcian Blue pH 2,5, Alcian Blue pH 2,5, PAS + Salivar Amilase, Method of Lars Grimelius and Method of Lillie. It was evidenced the predominance of serous cells on Duvernoy's gland. In relation to the histochemical constituion of the duvernoy's gland, was verified the presence of glucoconjugates neuters, enzymes and cells with reduetive activity. By fact from this species to kill his preys by constriction, the predominance of serous cells in the duvernoy's gland, don' t must be direct related with the subjugation of his preys, but with the digestive processes. Histological and histochemical analysis showed cells of Duvernoy's gland are constituted for two populations: serous cells (prodution of enzymes and mucous cells (prodution of glucoconjugates.

  6. Wie sich die Bilder gleichen – und die Geister scheiden: Mainstream-Ergebnisse in einem keynesianischen (? Lehrbuchmodell

    Directory of Open Access Journals (Sweden)

    Rainer Bartel

    2014-03-01

    Full Text Available Der ökonomische Mainstream (Orthodoxie und seine Alternativen (Heterodoxie werden hier grob in Neoklassik und Keynesianismus eingeteilt und kontrastiert. Ausgangspunkt ist die kognitive Dissonanz zwischen Mainstream-Theorie und Wirtschaftssystem-Realität. Es gibt aber auch breit verwendete Lehrbücher der Makroökonomik, die sich als fortschrittlich, weil keynesianisch fundiert, verstehen. Ich untersuche ein solches Modell: jenes von Blanchard et al. (2010, das ich das Blanchard-Modell nenne. Nach einer Charakterisierung und einem Vergleich des neoklassischen Arbeitsmarktmodells und seiner realwirtschaftlichen Implikationen mit dem Keynes’schen Modell des Gütermarktes und seinen Konsequenzen für den Arbeitsmarkt wird das Blanchard-Modell betrachtet und sein Fortschrittlichkeitsanspruch beurteilt. Es stellt sich heraus, dass das Blanchard-Modell dem neoklassischen Gleichgewichtsmodell sehr stark verhaftet ist und als kompatibel mit der Neoklassischen Synthese angesehen werden kann. Nach einem Fazit wird ein Ausblick auf mögliche Weiterent- wicklungen der Makroökonomik versucht.

  7. Wie gehen wir in der Vermittlung des Deutschen als Fremdsprache mit sprachlicher Qualität um?

    DEFF Research Database (Denmark)

    Colliander, Peter

    2011-01-01

    In der Pädagogik/Didaktik des Deutschen als Fremdsprache wird die erste Dichotomie erfahrungsgemäß in den Mittelpunkt gestellt, was zu einem rigiden Umgang mit dem Begriff „sprachliche Qualität“ führt, einem Umgang, der für die Motivation der Lerner nicht förderlich ist. Dieser ersten Dichotomie ...

  8. Big like a gym. New business models for public utilities; Gross wie eine Turnhalle. Neue Geschaeftsmodelle fuer Stadtwerke

    Energy Technology Data Exchange (ETDEWEB)

    Schwarzburger, Heiko

    2013-10-01

    Electricity storage is no longer a vision, also in the case of greater power ratings too. The breakthrough is done: ready-to-connect systems are available as complete packages or containers. [German] Stromspeicher sind keine Vision mehr. Auch nicht fuer grosse Leistungen. Der Durchbruch ist geschafft: Anschlussfertige Systeme sind als Container oder Gesamtpakete verfuegbar.

  9. Endocrine cells and nerve ganglia of the small intestine of the Opossum Didelphis aurita Wied-Neuwied, 1826 (Mammalia: Didelphidae

    Directory of Open Access Journals (Sweden)

    Gláucia M. Freitas-Ribeiro

    2012-09-01

    Full Text Available The nervous and endocrine systems jointly control intestinal movements, secretions of their glands and also participate of the processes of nutrient digestion and absorption. Therefore, the central objective of this study was to verify the existence of a possible relationship between the number of nervous cells and ganglia of the submucosal and myenteric plexuses and the number of endocrine cells in the small intestine of adult D. aurita. The utilized staining techniques were Grimelius, modified Masson-Fontana, direct immunoperoxidase and H-E. Argyrophillic, argentaffin and insulin immunoreactive endocrine cells do not numerically vary between the initial, mid and final regions of the duodenum, jejunum and ileum (P>0.05, except for argyrophillic cells in the jejunum (P>0.05. No numerical relationship has yet been verified between the number of nerve ganglia and endocrine cells, and also between nervous and endocrine cells. We recommended the use of new immunohistochemical techniques to confirm the numerical correlation between the nervous and endocrine systems in the small intestine. The morphology and distribution of endocrine cells and the nerve ganglia studied were similar to those encountered in eutherian mammals.Os sistemas nervoso e endócrino controlam integra-damente os movimentos intestinais, a secreção de suas glândulas e também participam dos processos de digestão e absorção de nutrientes. Portanto, o objetivo central deste estudo foi verificar a existência de uma possível relação entre o número de células nervosas e gânglios dos plexos submucosos e mioentéricos e o número de células endócrinas no intestino delgado de adultos de D. aurita. As técnicas de coloração utilizadas foram Grimelius, Masson-Fontana modificada, imunoperoxidase direta e H-E. As células endócrinas argirófilas, argentafins e imunorreativas à insulina não variaram numericamente entre as regiões inicial, média e final do duodeno, jejuno e íleo (P>0,05, exceto as células argirófilas no jejuno (P<0,05. Nenhuma relação numérica foi verificada entre o número de gânglios nervosos e células endócrinas, e também entre células nervosas e endócrinas. Nós recomendamos o emprego de novas técnicas imunohistoquímicas para confirmar a correlação numérica entre os sistemas nervoso e endócrino no intestino delgado. A morfologia e a distribuição das células endócrinas e dos gânglios nervosos estudados foram similares àqueles encontrados em mamíferos eutérios.

  10. Eye lens surveillance. What is the reaction of international organizations?; Ueberwachung der Augenlinse. Wie reagieren die internationalen Organisationen?

    Energy Technology Data Exchange (ETDEWEB)

    Behrens, R. [Physikalisch-Technische Bundesanstalt (PTB), Braunschweig (Germany)

    2013-07-01

    The ICRP has lowered its recommendation for the limit of the dose to the lens of the eye for occupationally exposed persons down to 20 mSv per year on average (mean value of 5 years with 50 mSv per year at maximum); both the European Commission and the IAEA have adopted this reduction in their respective basic safety standards. Even before this (and still more since then) several international activities were started (among others): The ICRP adopted a stylised model of the eye lens for the calculation of dose conversion coefficients for their report ICRP 116. The European Commission funded the ORAMED project dealing with radiation protection in medicine. The IEC fixed requirements for Hp(3) eye dosemeters in their standard IEC 62387 on passive dosimetry systems. The ISO and the IAEA give a variety of practical advice in the standard ISO 15382 and in a technical document TecDoc (both still drafts) for both radiation protection as well as dosimetry. The ICRU recommends both phantoms, the slab and the cylinder, for most cases. In short: Most national procedures can orientate themselves by referring to international ones; however, some questions remain open. (orig.)

  11. Tagungsbericht: Wie viel Kultur verträgt die Psychologie? "100 Jahre Deutsche Gesellschaft für Psychologie"

    OpenAIRE

    2005-01-01

    Anlässlich des 44. Kon­gres­ses der Deutschen Gesellschaft für Psychologie in Göttingen traten erstmals vermehrt und sichtbar Kulturpsychologen auf, die über die Thematik des Kulturvergleichs durch die internationalen Vergleichsstudien zu Schulleistungen (TIMS, IGLU, PISA) die Gegenstandsunangemessenheit der variablenorientierten Individualpsychologie sowie deren Methodologien und Methoden thematisierten. Zentrale Themen waren hierbei vor allem, die Äqui­valenzproblematik (tertium comparation...

  12. How to prevent legionella infestation. Pt. 1. Central freshwater heating systems; Wie man Legionellen vermeiden. T. 1. Zentrale Trinkwassererwaermungsanlagen

    Energy Technology Data Exchange (ETDEWEB)

    Kremer, Robert [DVGW-Fachausschuss Trinkwasserhygiene in Gebaeuden, Leverkusen (Germany)

    2009-01-15

    The contribution focuses on hygienically safe freshwater heating. Legionella prophylaxis is discussed, as are new findings concerning practical implementation of new requirements on modern freshwater supply systems. (orig.)

  13. Wie sensibel reagieren deutsche Verbraucher auf Preisänderungen bei Bio-Eiern? Eine Nachfrageanalyse mit Haushaltspanel-Daten

    OpenAIRE

    Schroeck, Rebecca

    2011-01-01

    Die Studie analysiert die Nachfrage nach Eiern aus konventioneller und ökologischer Erzeugung in Deutschland auf der Basis von Daten des Haushaltspanels der Gesellschaft für Konsumforschung (GfK) für frische Lebensmittel. Die Datengrundlage ist in ihrer Größe und ihrem Informationsgehalt einzigartig, da sie Einkäufe von mehr als 13.000 Haushalten über einen Fünfjahreszeitraum abbildet. Es wird eine zweistufiges Schätzverfahren angewandt. Im ersten Schritt wird mit einer Probit- Analyse unters...

  14. Artérias mesentéricas cranial e caudal em mocós Kerodon rupestris (Wied, 1820

    Directory of Open Access Journals (Sweden)

    Paula V.S. Queiroz

    2011-07-01

    Full Text Available No estudo sobre a origem e ramificações das artérias mesentéricas cranial (AMCr e caudal (AMCa do mocó, foram utilizados 20 animais (18 machos e 2 fêmeas de diferentes idades, que, após morte natural, foram dissecados rebatendo-se as paredes torácica e abdominal, pelo antímero esquerdo, expondo-se a aorta que foi então canulada em seu trajeto pré-diafragmático, procedendo-se a injeção de neoprene látex corado, no sentido caudal. A seguir, foram fixados em solução aquosa de formol a 10%, durante 48 horas, e posteriormente dissecados. Os resultados mostraram que em 18 animais (90%, a AMCr originou-se da aorta abdominal isoladamente, logo após a artéria celíaca, emitindo as artérias cólica média (CoM, pancreaticoduodenal caudal (PDC, duodenojejunal (DJ, jejunal (J e ileocecocólica (ICeCo. Em um mocó (5%, as AMCr e AC se originaram da aorta abdominal em um tronco comum. Neste caso, a AMCr originou às artérias CoM, PDC, ICeCo e J. Em uma observação (5%, as artérias AMCr e AMCa surgiram em tronco comum. Neste animal, as artérias PDC, DJ, ICeCo, CoM e J foram originadas da AMCr, enquanto as aterias cólica esquerda (CoE e retal cranial (ARCr derivaram da AMCa. Dois animais (10% apresentaram como colaterais da AMCr as artérias CoM, PDC, DJ, J e o tronco ICeCo, que originou às artérias CoD e ileocecal (ICe. No que diz respeito a AMCa, nos 20 casos (100% originou as artérias CoE e RCr.

  15. Complete CRM documentation as a basis for successfull Churn Management; Wie aus Rueckholung Vorbeugung wird. Vollstaendige Kundenkontakthistorie als Basis

    Energy Technology Data Exchange (ETDEWEB)

    Johannssen, T. [ConTakt GmbH, Itzehoe (Germany)

    2002-09-09

    The contribution explains modern customer relationship management strategies for electric utilities. The role of a complete and detailed documentation of the CRM history is explained as a basis for establishing reliable customer loyalty, winning back lost customers, or preventing customers from changing their power service. (orig./CB) [German] Erkenntnisse aus der Kundenrueckgewinnung sind eine wertvolle Grundlage fuer Kuendigungspraevention. Bei durchdachtem Vorgehen koennen sie mit den gesammelten Erfahrungen sogar schon im Vorfeld Kuendigungen verhindern. (orig./CB)

  16. Sourcing, regie en verandering in IT organisaties : Het gaat alle kanten op en wie weet nog hoe het zit?

    NARCIS (Netherlands)

    Willems, Frank

    2012-01-01

    Lezing voor NGI Noord (Platform voor ICT-professionals) op 9 oktober 2012, over de actuele trends rond sourcing, regie en veranderen, aan de hand van praktijkvoorbeelden. Wat betekent dit voor IT professionals persoonlijk en hoe wordt en blijf je innovatief en verandergericht. Bijeenkomst georganise

  17. Review: Christine Feil, Regina Decker & Christoph Gieger (2004. Wie entdecken Kinder das Internet? [How Do Children Discover the Internet?

    Directory of Open Access Journals (Sweden)

    Thomas Link

    2006-01-01

    Full Text Available This book deals with the following questions: When and where do children use the Internet? What search strategies do they deploy? What difficulties do they encounter when surfing the web? In order to answer these questions, the authors interviewed parents and instructors about children’s Internet use. They also observed children surfing the Internet, though these observations were made in a research laboratory-like setting rather than in a natural context. In addition to the results of this empirical study, the book includes a presentation of German-language websites suitable for children and a discussion of the protection of children and young people on the Internet. The text is especially interesting when it becomes apparent, possibly in contradiction to common expectations, how little the Internet has to offer to children apart from commercial web sites. The study is to be understood as an explorative study and therefore lacks a certain theoretical depth. It offers a good overview of the (German-language Internet for children and allows insights into possible ways in which children deal with the Internet. URN: urn:nbn:de:0114-fqs0601103

  18. Von Systemkritik zur Anpassung : Wie ein Buch in der Übersetzung die Farbe wechselt / Marianne Vogel

    Index Scriptorium Estoniae

    Vogel, Marianne

    2002-01-01

    Arvustus: Vetemaa, Enn. Die Nixen von Estland : ein Bestimmungsbuch / frei nach E. Vetemaa ; bearbeitet und illustriert von Kat Menschik ; nach dem russischen Übersetzungsmanuskript ins Deutsche übertragen von Günter Jäniche. Frankfurt/M : Eichborn Verlag, 2002.

  19. The Electricity Feed Law levy - how reliably can it be predicted?; Wie verlaesslich laesst sich die EEG-Umlage prognostizieren?

    Energy Technology Data Exchange (ETDEWEB)

    Bause, Rainer; Schulz, Woldemar [Amprion GmbH, Dortmund (Germany); Buehler, Holger [EnBW TNG, Stuttgart (Germany); Hodurek, Claus [50Hertz Transmission GmbH, Berlin (Germany); Kiessling, Axel [TenneT TSO GmbH, Bayreuth (Germany)

    2011-10-15

    By paying their monthly electricity bill German consumers are promoting the transition to tomorrow's resource-efficient electricity supply system, in which the largest part of electricity used is to come from renewable energy resources. Every year Germany's transmission system operators publish what is referred to as the ''EEG-Umlage'' (report on the Electricity Feed Law levy, or EEG levy), which shows how much every German household will be paying for the promotion of renewable energies in the coming year. The determination of the EEG levy involves uncertainties and imponderabilities which have to be taken into account in its calculation. The crucial task is to find a suitable systematic scheme for predicting the renewable energy yield.

  20. WIE PREISSENSIBEL REAGIEREN DEUTSCHE VERBRAUCHER? AKTUELLE SCHÄTZUNGEN VON PREIS- UND AUSGABENELASTIZITÄTEN AUF DER BASIS VON HAUSHALTSPANELDATEN

    OpenAIRE

    2012-01-01

    Der Beitrag untersucht das Nachfrageverhalten der deutschen Privathaushalte anhand von Daten zweier Haushaltspanels der Gesellschaft für Konsumforschung. Es wird ein Almost Ideal Demand System (AIDS) mit 13 Lebensmittelgruppen für den Zeitraum von 2004 bis 2008 geschätzt. Der Fokus der Analyse liegt auf den geschätzten Eigenpreis- und Ausga-benelastizitäten und deren Einordnung in die Literatur. Mit Ausnahme von Kaffee und Eiern zeigt sich die Nachfrage in allen Lebensmittelgruppen, darunter ...