WorldWideScience

Sample records for censo sui generis

  1. Hostos y el Positivismo sui Generis Latinoamericano

    Directory of Open Access Journals (Sweden)

    Pablo Guadarrama González

    2004-01-01

    Full Text Available El positivismo fue asumido y cultivado en Latinoamérica de forma sui generis como una filosofía optimista llena de confianza en el hombre, en la capacidad creativa de su pensamiento, en la cultura, la educación, la ciencia, el progreso y el desarrollo industrial. Aliado al liberalismo y a la defensa de la democracia sus ideas resultaban muy avanzadas para los países latinoamericanos, recién liberados en su mayoría del colonialismo español y enfrascados entonces en profundas luchas entre las oligarquías retrogradas y la naciente burguesía nacional. Las ideas filosóficas y educativas de Hostos ponen de manifiesto la riqueza alcanzada por el pensamiento latinoamericano en el siglo XIX que aunque compartió algunas posiciones con el krausismo y el positivismo no se dejó arrastrar de forma unilateral por estas corrientes y supo aprovechar los elementos de valor contenidos especialmente en el positivismo que supo asumir de modo sui generis como la mayoría de los intelectuales latinoamericanos de esa época que se identificaron con él en correspondencia con una praxis educativa y política beneficiosa a los pueblos de América Latina.

  2. DIPLOMASI TAKHTA SUCI SEBAGAI SUBJEK HUKUM INTERNASIONAL SUI GENERIS

    Directory of Open Access Journals (Sweden)

    Agustinus Supriyanto

    2015-02-01

    Full Text Available The Holy See is different from the Vatican. The Vatican is a state, whereas the Holy See is the central governing institution of the Catholic Church. The position of the Holy See is in the Vatican State. The international status of the Holy See is very unique. It is a sui generis subject of international law. Although it is not a state, it enjoys international recognition. Its diplomacy consists of intern and extern aspect. The intern aspect of its diplomacy is that of church interest. The extern aspect is that of diplomacy itself. It is neutral in internationally political interests. It focuses on humanitarian and moral diplomacy.

  3. Effectiveness of REDD programs in the protection of sui generis indigenous rights

    Directory of Open Access Journals (Sweden)

    Paloma Infante M.

    2013-06-01

    Full Text Available The article focuses on the implementation of the United Nations Reducing Emission from Deforestation and Forest Degradation Program, hereinafter UN REDD and its effectiveness in the protection of sui generis indigenous rights, meaning those different from the property right but derived from it, especially, the rights over the territories, natural resources and environmental services. The paper will cover the interconnection between sui generis indigenous rights and REDD programs including the relevance of land tenure and the sui generis rights derived from it. As we will emphasize, REDD programs are not only focused in the reduction of carbon emissions. In fact, REDD plus programs include as a main objective the sustainable management of forests and the protection to forest dependent communities.

  4. DATABASES AND THE SUI-GENERIS RIGHT – PROTECTION OUTSIDE THE ORIGINALITY. THE DISREGARD OF THE PUBLIC DOMAIN

    Directory of Open Access Journals (Sweden)

    Monica LUPAȘCU

    2018-05-01

    Full Text Available This study focuses on databases as they are regulated by Directive no.96/9/EC regarding the protection of databases. There are also several references to Romanian Law no.8/1996 on copyright and neighbouring rights which implements the mentioned European Directive. The study analyses certain effects that the sui-generis protection has on public domain. The study tries to demonstrate that the reglementation specific to databases neglects the interests correlated with the public domain. The effect of such a regulation is the abusive creation of some databases in which the public domain (meaning information not protected by copyright such as news, ideas, procedures, methods, systems, processes, concepts, principles, discoveries ends up being encapsulated and made available only to some private interests, the access to public domain being regulated indirectly. The study begins by explaining the sui- generis right and its origin. The first mention of databases can be found in “Green Paper on Copyright (1998,” a document that clearly shows, the database protection was thought to cover a sphere of information non-protectable from the scientific and industrial fields. Several arguments are made by the author, most of them based on the report of the Public Consultation sustained in 2014 in regards to the necessity of the sui-generis right. There are some references made to a specific case law, namely British Houseracing Board vs William Hill and Fixture Marketing Ldt. The ECJ’s decision în that case is of great importance for the support of public interest to access information corresponding to some restrictive fields that are derived as a result of the maker’s activities, because in the absence of the sui-generis right, all this information can be freely accessed and used.

  5. Hume, Mill, Hill, and the sui generis epidemiologic approach to causal inference.

    Science.gov (United States)

    Morabia, Alfredo

    2013-11-15

    The epidemiologic approach to causal inference (i.e., Hill's viewpoints) consists of evaluating potential causes from the following 2, noncumulative angles: 1) established results from comparative, observational, or experimental epidemiologic studies; and 2) reviews of nonepidemiologic evidence. It does not involve statements of statistical significance. The philosophical roots of Hill's viewpoints are unknown. Superficially, they seem to descend from the ideas of Hume and Mill. Hill's viewpoints, however, use a different kind of evidence and have different purposes than do Hume's rules or Mill's system of logic. In a nutshell, Hume ignores comparative evidence central to Hill's viewpoints. Mill's logic disqualifies as invalid nonexperimental evidence, which forms the bulk of epidemiologic findings reviewed from Hill's viewpoints. The approaches by Hume and Mill cannot corroborate successful implementations of Hill's viewpoints. Besides Hume and Mill, the epidemiologic literature is clueless about a plausible, pre-1965 philosophical origin of Hill's viewpoints. Thus, Hill's viewpoints may be philosophically novel, sui generis, still waiting to be validated and justified.

  6. Contractual framework of private-public partnership: The sui generis nature of PPP as a result of the holistic approach

    Directory of Open Access Journals (Sweden)

    Cvetković Predrag

    2014-01-01

    Full Text Available The holistic (comprehensive] approach to the methodological framework for the analysis of public-private partnership is essential presumption for the transformation of the public and private interests from the conflicting ones to the parallel and convergent ones. Private and public interests are to be regarded not as the divided particulars but as the element of the dynamics of PPP as a whole. The holistic approach facilitates interaction which brings new 'added values' in the exercise of the public interest by using the PPP construction: economic, social and political. This concept allows the PPP to 'take the best of both worlds' (the protection of the public interest and solidarity from the public sector, and the entrepreneurial logic from the private sector]. In light of the sui generis structure of mutual relations between the public and the private partner in the PPP framework, the contractual basis of public-private partnership has the character of relational contracts. Relational contracts differ from traditional contracting mechanisms. The parties to traditional contracts have equal information at their disposal, which enables them to define ex ante the terms of the transaction insofar as the consequences of performance or non-performance are anticipated at the time of contract conclusion. On the other hand, relational contracts are incomplete agreements: the parties to relational contracts mutually agree that it is impossible or economically inefficient to define ex ante the possible difficulties and circumstances of the transaction at issue (which is possible in classical contracts]. In this respect, in relational contracts the rationale for commitment is relatively flexible. The public-private partnership agreement is a relational contract. Relational contracts within the PPP framework limit the opportunistic behaviour on both parties and promote the development of their co-operation and exchange of information. Relational contracts

  7. Censo 2010

    Directory of Open Access Journals (Sweden)

    Eliud Silva

    2013-01-01

    Full Text Available En este artículo se utilizan series de tiempo multivariadas para mostrar que es factible realizar pronósticos de población con esta perspectiva y se evidencia cómo éstos son satis- factorios de acuerdo con datos derivados del Censo de Población y Vivienda 2010 para la Zona Metropolitana de la Ciudad de México (zmcm. Se enuncia de manera general la metodología empleada para realizar los pronósticos partiendo de técnicas de desagre- gación, ajustes y estimación de pronósticos multivariados. Se considera que las herra- mientas metodológicas capaces de proyectar de manera eficiente resultan indispensables para elaborar gran diversidad de programas públicos cuyo principal objetivo sea un desarrollo sustentable.

  8. Streptococcus suis

    DEFF Research Database (Denmark)

    Poggenborg, René; Gaïni, Shahin; Kjaeldgaard, Poul

    2008-01-01

    Meningitis and spondylodiscitis caused by Streptococcus suis is a rare disease which is contracted by occupational exposure to pigs. We report a 54-y-old pig-farm worker with S. suis meningitis and septicaemia complicated with thoracal and lumbar spine spondylodiscitis. The S. suis strain involved...

  9. Los censos : concepto y naturaleza

    Directory of Open Access Journals (Sweden)

    Adolfo Ballester Martínez

    2005-01-01

    Full Text Available El contrato de censo otorga un marco legal a una actividad económica que puede consistir en el traspaso del derecho de uso, en el enfitéutico; la venta de una propiedad, en el consignativo impropio y el reservativo; la concesión de un préstamo de dinero con interés en el consignativo propio. El marco legal creado por este tipo de contrato se caracteriza por la existencia de unas cargas reales sobre las propiedades vinculadas, generando derechos y obligaciones de larga duración a las partes. El censualista tiene garantizados sus derechos de propiedad, sobre bienes raíces, inmuebles o capital, por medio del cobro anual de la pensión fijada en el momento de la formalización del contrato, la posibilidad del tanteo ante la posible enajenación de la finca por parte del censatario y la ejecución de las hipotecas ofrecidas como garantía del pago de la pensión por parte del censatario, en los contratos consignativos, o la consolidación de los dominios, en los enfitéuticos, en caso de impago de la renta durante tres años consecutivos.The leasehold contract («censo» creates a legal boundary that consists of the right of use, in the contract called «enfitéutico», the sale of a property in the «consignativo impropio» and the «reservativo»; the concesion of a loan with interests in the «consignativo propio». The legal boundaries created by this type of contract is characterized by the existence of duties over the mortgaged properties, generating both rights and obligations to the tenants over a long period. The landlord («censualista» has guaranteed property rights on land, property and capital, by means of an annual payment of the fixed pension at the moment of formalizing the contract. The possibility of equalling the possible sale price of the property by a third party, the use of mortgages as guarantees of payment on behalf of the landlord in the contracts called «consignativos» or the consolidation of the properties (

  10. NÓS CONTADORES, POSSUÍMOS UM PERFIL SUI GENERIS DE INTELIGÊNCIAS?

    Directory of Open Access Journals (Sweden)

    Márcia Athayde Matias

    2012-04-01

    Full Text Available This research studied the multiple intelligences of accountants, trying to identify a profile or mainstream of intelligences among these professionals. For this, was used the theory of Howard Gardner, who argues that the concept of intelligence goes beyond the historical paradigms that have been associated only with the mental capacity of a logical-mathematical or linguistic, since many mental skills are used both by people who have different vocations according to their dominant intelligences. The set of intelligences described by Gardner are the logicalmathematical intelligence, linguistic, bodily-kinesthetic, spatial, musical, naturalist, interpersonal and intrapersonal intelligences. Defined as a descriptive study, was conducted a survey with a questionnaire to collect data, which was answered by 189 accountants. Outlining the profile accountants showed capacity of understanding, logical reasoning, no difficulty in analysis and a kind of systematic behavior. Moreover, they demonstrated ability to work in groups and be empathic. The lack of language skills caused some frustration and concern about the accounting professional. Moreover, it was emphasized with the Pearson correlation analysis that therelationship between the intelligences are not strong, the highest score was 0.506 between the logical-mathematical intelligence and spatial intelligence, among other interesting relationships. Concluded that patterns revealed by research are an important tool to discuss methods to stimulate the cognitive development of accountants, with direct consequences on accounting profession.

  11. Streptococcus suis infection

    Science.gov (United States)

    Feng, Youjun; Zhang, Huimin; Wu, Zuowei; Wang, Shihua; Cao, Min; Hu, Dan; Wang, Changjun

    2014-01-01

    Streptococcus suis (S. suis) is a family of pathogenic gram-positive bacterial strains that represents a primary health problem in the swine industry worldwide. S. suis is also an emerging zoonotic pathogen that causes severe human infections clinically featuring with varied diseases/syndromes (such as meningitis, septicemia, and arthritis). Over the past few decades, continued efforts have made significant progress toward better understanding this zoonotic infectious entity, contributing in part to the elucidation of the molecular mechanism underlying its high pathogenicity. This review is aimed at presenting an updated overview of this pathogen from the perspective of molecular epidemiology, clinical diagnosis and typing, virulence mechanism, and protective antigens contributing to its zoonosis. PMID:24667807

  12. Meningitis por Streptococcus suis

    OpenAIRE

    Geffner Sclarsky, D. E.; Moreno Muñoz, R.; Campillo Alpera, Mª.S.; Pardo Serrano, F.J.; Gómez Gómez, A.; Martínez-Lozano, Mª.D.

    2001-01-01

    La infección humana por Streptococcus suis (S. suis) es una zoonosis, con un riesgo ocupacional conocido y que suele presentarse como meningitis purulenta, que tiene baja mortalidad y frecuentes secuelas de hipoacusia y ataxia. Se han publicado menos de 150 casos humanos desde el informe original de hace 30 años. Hay una reconocida distribución geográfica viviendo la mayoría de los afectados en el norte de Europa y el sudeste Asiático. En España se han comunicado dos pacientes con enfermedad ...

  13. SUI CODICI UNIDIREZIONALI

    Directory of Open Access Journals (Sweden)

    Luca Tallini

    1992-04-01

    Full Text Available Oltre ad una breve descrizione della problematica sui codici Unidirezionali è qui data la dimostrazione di una congettura di S. Al-Bassan e B. Bose che interviene nella teoria dei suddetti autori, sulla costruzione di codici ottimali nella classe dei codici bilanciati che si ottengono con il metodo della complementazione di Knuth.

  14. Contractual framework of private-public partnership: The sui generis nature of PPP as a result of the holistic approach

    OpenAIRE

    Cvetković Predrag

    2014-01-01

    The holistic (comprehensive] approach to the methodological framework for the analysis of public-private partnership is essential presumption for the transformation of the public and private interests from the conflicting ones to the parallel and convergent ones. Private and public interests are to be regarded not as the divided particulars but as the element of the dynamics of PPP as a whole. The holistic approach facilitates interaction which brings new 'added values' in the exercise of the...

  15. Current Taxonomical Situation of Streptococcus suis.

    Science.gov (United States)

    Okura, Masatoshi; Osaki, Makoto; Nomoto, Ryohei; Arai, Sakura; Osawa, Ro; Sekizaki, Tsutomu; Takamatsu, Daisuke

    2016-06-24

    Streptococcus suis, a major porcine pathogen and an important zoonotic agent, is considered to be composed of phenotypically and genetically diverse strains. However, recent studies reported several "S. suis-like strains" that were identified as S. suis by commonly used methods for the identification of this bacterium, but were regarded as distinct species from S. suis according to the standards of several taxonomic analyses. Furthermore, it has been suggested that some S. suis-like strains can be assigned to several novel species. In this review, we discuss the current taxonomical situation of S. suis with a focus on (1) the classification history of the taxon of S. suis; (2) S. suis-like strains revealed by taxonomic analyses; (3) methods for detecting and identifying this species, including a novel method that can distinguish S. suis isolates from S. suis-like strains; and (4) current topics on the reclassification of S. suis-like strains.

  16. Relatorio do Censo Brasileiro de Dialise Cronica 2012

    Directory of Open Access Journals (Sweden)

    Ricardo Cintra Sesso

    2014-03-01

    Full Text Available Introdução: Dados nacionais sobre diálise crônica são fundamentais para o conhecimento e planejamento do tratamento. Objetivo: Apresentar dados do censo da Sociedade Brasileira de Nefrologia sobre os pacientes com doença renal crônica em diálise em julho de 2012. Métodos: Levantamento de dados de unidades de diálise de todo o país. A coleta de dados foi feita utilizando questionário preenchido on-line pelas unidades de diálise do Brasil. Resultados: 255 (39,1% unidades responderam ao censo. Em julho de 2012, o número total estimado de pacientes em diálise no país foi de 97.586. As estimativas nacionais das taxas de prevalência e de incidência de doença renal crônica em tratamento dialítico foram de 503 e 177 pacientes por milhão da população, respectivamente. O número de pacientes que iniciaram tratamento em 2012 foi 34.366. A taxa anual de mortalidade bruta foi de 18,8%. Dos pacientes prevalentes, 31,9% tinham idade ≥ 65 anos, 91,6% estavam em hemodiálise e 8,4% em diálise peritoneal, 30.447 (31,2% estavam em fila de espera para transplante, 28,5% tinham diabetes, 36,6% tinham fósforo sérico > 5,5 mg/dl e 34,4% hemoglobina < 11 g/dl. Cateter venoso era usado como acesso em 14,5% dos pacientes em hemodiálise. Conclusão: As taxas de prevalência e incidência de pacientes em diálise aumentaram, e a taxa de mortalidade tendeu a diminuir em relação a 2011. Os dados de indicadores da qualidade da diálise de manutenção encontram-se estáveis com tendência à queda nos níveis de anemia; e mostram a relevância do censo anual para o planejamento da assistência dialítica.

  17. Censo Brasileiro de Diálise, 2009

    Directory of Open Access Journals (Sweden)

    Ricardo de Castro Cintra Sesso

    2010-12-01

    Full Text Available INTRODUÇÃO: Dados nacionais sobre diálise crônica são fundamentais para o planejamento do tratamento. OBJETIVO: Apresentar dados do censo anual da SBN sobre os pacientes com doença renal crônica em diálise de manutenção a 1º de janeiro de 2009. MÉTODOS: Levantamento de dados de unidades de diálise de todo o país. A coleta de dados foi feita utilizando questionário preenchido pelas unidades de diálise do Brasil cadastradas na SBN. RESULTADOS: Das unidades consultadas, 437 (69,8% responderam ao censo. Em janeiro de 2009, o número estimado de pacientes em diálise foi de 77.589. As estimativas das taxas de prevalência e de incidência de insuficiência renal crônica em tratamento dialítico foram de 405 e 144 pacientes por milhão da população, respectivamente. O número estimado de pacientes que iniciaram tratamento em 2009 foi de 27.612. A taxa anual de mortalidade bruta foi de 17,1%. Dos pacientes prevalentes, 39,9% tinham idade > 60 anos, 89,6% estavam em hemodiálise e 10,4% em diálise peritoneal, 30.419 (39,2% estavam em fila de espera para transplante, 27% eram diabéticos, 37,9% tinham fósforo sérico > 5,5 mg/dL e 42,8% hemoglobina < 11 g/dL. Cateter venoso foi usado como acesso vascular em 12,4% dos pacientes em hemodiálise. CONCLUSÕES: A prevalência de pacientes em diálise tem apresentado aumento progressivo embora em 2009 as estimativas sejam inferiores às de 2008. Os dados chamam atenção para indicadores da qualidade diálise de manutenção que necessitam ser melhorados. E destacam a importância do censo anual para o planejamento da assistência dialítica.

  18. Definición de un modelo de diseño para censos poblacionales

    OpenAIRE

    Quiroga, Graciela Alejandra; Ortiz Banda, Úrsula Italia

    2005-01-01

    El punto de partida de este trabajo fue generar un modelo para la manipulación de censos. Para ello, investigamos sobre Sistemas de Información Geográfica, en adelante SIG, y su aplicación sobre censos, para los cuales fue necesario obtener definiciones precisas, debido a que en el tema muchas palabras como por ejemplo hogar que para nosotros es una palabra común en el contexto de un censo tiene muchos significados. Además nos fue necesario encontrar un problema real que afecte a la socied...

  19. La realización del censo de Floridablanca en el corregimiento de Murcia

    Directory of Open Access Journals (Sweden)

    Juan HERNÁNDEZ FRANCO

    2009-12-01

    Full Text Available Cuando se examina la validez demográfica de un censo, por lo general —y en el caso del censo de Floridablanca lo podemos comprobar— se procede a determinarla mediante el cálculo de las tasas demográficas y la concordancia de densidades de población. Indudablemente la fiabilidad será mayor si conocemos de forma rigurosa el proceso de ejecución del censo. Es decir, normas, reglas, instituciones encargadas o creadas para su confección y cómo la gestionaron, errores cometidos, corrección de las mismas, etc. Todos estos factores son precisos para determinar con mayor seguridad la fiabilidad de un censo.

  20. Analfabetismo e níveis de letramento no Brasil: o que dizem os censos?

    Directory of Open Access Journals (Sweden)

    Ferraro Alceu Ravanello

    2002-01-01

    Full Text Available O texto começa discutindo a velha questão da qualidade das estatísticas educacionais e alguns aspectos metodológicos relacionados com a utilização destas na pesquisa em educação. Analisa a seguir o analfabetismo, focalizando: a a sua emergência como problema político no final do período imperial; b a evolução do conceito; c a tendência secular, em números porcentuais e absolutos, desde o primeiro censo em 1872 até o Censo 2000. Por último, com base no Censo 2000, classifica a população em diferentes níveis de letramento.

  1. La calidad de los datos de mortalidad del Censo 2010 de Argentina

    Directory of Open Access Journals (Sweden)

    Nicolás Sacco

    2016-07-01

    Full Text Available Objetivo: evaluar las variables disponibles en el censo de población de 2010 para Argentina en lo que a mortalidad refiere. Métodos: Con base en métodos indirectos de estimación demográfica se ofrece una lectura de la calidad de estas estadísticas en comparación con censos previos y estadísticas vitales. Resultados: La comparación de las tasas de mortalidad infantil y adulta con las tasas calculadas a partir de los nacimientos y las defunciones permite dilucidar, en principio, que estos datos no revelan problemas serios de calidad o cobertura y que el nivel (y la tendencia indicado por las fuentes es ampliamente compatible. Discusión: a pesar de que los resultados parecen mostrar coherencia, la posibilidad de detectar y cuantificar los errores se mantiene como un punto ciego del artículo hasta tanto no se publique la totalidad de la información necesaria para realizar un examen cabal, dado el universo limitado de publicaciones por parte del Instituto Nacional de Estadística y Censos. Conclusiones: las estimaciones de población en cuanto a mortalidad son consistentes con los datos del censo previo de 2001 y con las series de nacimientos y muertes del período intercensal a nivel total del país.

  2. Streptococcus suis meningitis, a poacher's risk

    NARCIS (Netherlands)

    Halaby, T.; Hoitsma, E.; Hupperts, R.; Spanjaard, L.; Luirink, M.; Jacobs, J.

    2000-01-01

    Streptococcus suis infection is a zoonosis that has been mainly reported in pig-rearing and pork-consuming countries. The most common disease manifestation is meningitis, often associated with cochleovestibular signs. The causative agent is Streptococcus suis serotype 2, found as a commensal in the

  3. Inventarios del deseo. Los censos municipales de Rosario, Argentina (1889-1910: Municipal census of Rosario, Argentina (1889-1910

    Directory of Open Access Journals (Sweden)

    Diego P. Roldán

    2013-06-01

    Full Text Available Este artículo propone una aproximación a los censos municipales de Rosario, considerando la estadística como un género de escritura y el proceso de formación histórica de la promoción y proto-marketing urbano. Se estudia la producción y circulación de censos de la provincia de Santa Fe y la ciudad de Rosario desde 1887 hasta 1910. La primera parte del trabajo se concentra en el proceso de difusión del primer censo provincial de Santa Fe en Francia, durante la exposición universal de 1889. Luego se reconstruyen las distintas tentativas de construir oficinas municipales de estadísticas en la ciudad de rosario, movilizadas por la trayectoria del censo de 1887, las crisis sanitarias y los preparativos del censo nacional de 1895. El centro del trabajo considera los censos municipales de rosario de 1900, 1906 y 1910 procurando pasar del problema de la recolección-captación al de la fabricación invención de los datos y de la representatividad al de la performatividad de la estadística como proceso de producción social de saberes y del censo como artefacto cultural.

  4. La protección social de la dependencia en España. Un modelo sui generis de desarrollo de los derechos sociales

    Directory of Open Access Journals (Sweden)

    Gregorio Rodríguez Cabrero

    2008-01-01

    Full Text Available Social and demographic changes related to informal care model as well as the new idea of social risk have lead to a social and political debate which has concluded at least in Spanish case in a new branch of the Welfare State, in many ways a synthesis of informal care Mediterranean tradition, Continental family vision and Nordic universalism. The new social protection model involves the construction of a complex institutional building. Its management, coordination, financing and evaluation constitutes a test of a mixed welfare which will show the conflicts between the new social protection model, the financial limits of Welfare State and the decentralization of social policies. The development of the Social protection Dependency Law from 2007 onwards it is crucial in relation to the future of Spanish social reform in the first half present century.

  5. La costellazione dei generi: forme letterarie e modi del discorso nel pensiero di Paul Ricœur

    Directory of Open Access Journals (Sweden)

    Roberto Talamo

    2013-12-01

    Full Text Available Il pensiero filosofico di Ricœur si è sviluppato attraverso un costante e proficuo confronto con le diverse forme della letteratura antica e moderna. Con una carrellata attraverso i testi del filosofo e quelli dei principali interpreti della sua riflessione, si ricostruisce la trama significativa dei nessi tra filosofia e generi letterari: questi offrono al pensiero ricoeuriano la possibilità di «pensare di più» i nodi etici del campo filosofico.

  6. Sui Generis” Journalism? Visibility, Identities and Journalistic Practices in a 1990s Brazilian Gay Magazine

    Directory of Open Access Journals (Sweden)

    Ricardo Augusto de Saboia Feitosa

    2018-04-01

    Full Text Available This article examines the Sui Generis magazine (1995-2000, one of the most relevant gay press magazines in Brazil. We study the importance of this publication and the field of journalism in terms of producing and reproducing representations and processes of identities and relations of gender and sexuality. The methodology involves a discursive reading of editorials, cover stories and reader letters published between January 1995 and March 2000, as well as a restructuring of journalistic work and daily practices in newsrooms through interviews with reporters, columnists and editors. We reached the conclusion that choosing a policy of visibility based on outing and concepts of gay "identities" and "communities" allowed the magazine to create specific and more valued ways of what homosexuality is, which leads to a critical reflection on what these policies have achieved, and what their limits and tensions are. O artigo propõe uma investigação da revista Sui Generis (1995-2000, título do segmento especializado designado como “imprensa gay” brasileira. Busca-se compreender a relevância da publicação e deste campo jornalístico como instâncias historicamente produtoras e reprodutoras tanto de representações como dos processos de agenciamento de identidades e relações de gênero e sexualidade. Adota-se como metodologia a leitura discursiva de editoriais, reportagens de capa e cartas dos leitores publicados entre janeiro de 1995 e março de 2000; e a reconstituição, por meio de entrevistas com repórteres, colunistas e editores, do fazer jornalístico e das práticas cotidianas da redação. A análise permite constatar que, ao eleger como estratégia uma política de visibilidade calcada no outing e na elaboração de noções de “identidade” e “comunidade” gays, forjam-se modos específicos e mais valorizados do que seria a homossexualidade, exigindo uma reflexão crítica das conquistas dessas políticas, dos seus limites e

  7. Streptococcus suis meningitis in the Netherlands

    NARCIS (Netherlands)

    van de Beek, Diederik; Spanjaard, Lodewijk; de Gans, Jan

    2008-01-01

    We present four patients with Streptococcus suis meningitis identified during a 3.5-year prospective surveillance study in the Netherlands. All cases were associated with exposure to pigs. Patients presented with classic symptoms and signs of bacterial meningitis. Outcome was characterized by severe

  8. Censo estadounidense 2010: cifras e implicaciones de la mayor presencia de Centroamericanos en Estados Unidos

    Directory of Open Access Journals (Sweden)

    José Luis Rocha

    2011-12-01

    Full Text Available Con el tic-tac del reloj del censo estadounidense, este texto da cuenta de las cifras de migrantes centroamericanos en Estados Unidos: cuántos son, a qué ritmo crecen, dónde se ubican. Posteriormente, con base en el procesamiento de las estadísticas del Census Bureau 2010, muestra algunas características de los centroamericanos: son los más rezagados -en educación, arraigo, ingresos, obtención de ciudadanía, y de empleos estables y bien remunerados- entre los inmigrantes latinos. Sus patrones de asentamiento pueden repercutir negativamente sobre su futuro porque sus opciones geográficas son un voto político de los sin voto: contribuyen al crecimiento demográfico y de sillas en el congreso de los estados sureños, proverbial plaza de republicanos que han promovido leyes y operativos anti-inmigrantes.

  9. [Streptococcus suis infection--clinical manifestations].

    Science.gov (United States)

    Dragojlović, Julijana; Milosević, Branko; Sasić, Neda; Pelemis, Mijomir; Sasić, Milan

    2005-01-01

    Streptococcus suis is a bacterium causing a disease in pigs and rarely in humans. This zoonosis is mostly found as a sporadic disease in individuals that were in contact with the affected or infected pigs: farmers, veterinarians and workers engaged in fresh pork processing. It is assumed that the bacterium enters the body through a cut abrasion in the skin. Initially, the condition resembles a flu, followed by signs of bacteriemia and sepsis. The most frequent clinical manifestation of Streptococcus suis infection is meningitis, leading to hearing loss in over 75% of patients, and subsequent arthritis, endophtalmitis, endocarditis and pneumonia. Toxic shock syndrome with hemorhagic manifestations rarely develops. This study included five male patients aged 22 to 63 years treated in the Intensive Care Unit of the Institute of Infectious and Tropical Diseases in Belgrade, due to Streptococcus suis infection. The aim of this study was to point to the existence of this bacteria in our environment, to describe clinical manifestations of the disease and to point out the importance of its prevention. All patients had epidemiological evidence of being in contact with pork meat. There were no data about diseased pigs. The estimated incubation period was 4 to 8 days. All patients had meningeal signs. Clinical symptoms included shivering, fever, vomiting, headache, malaise, vertigo and tinitus. Three patients presented with alerterd level of awarrness. Four patients developed very severe bilateral hearing impairment, whereas one endophtalmtis and one developed endocarditis. The cerebrospinal fluid (CSF) was opalescent in four patients, and only one patient presented with clear CSF. CSF examination showed typical changes characteristic for bacterial meningitis. Streptoccocus suis was isolated in CSF in all patients, and in one patient the bacteria was isolated in blood as well. All patients underwent treatement with II and III generation cephalosporins and one with one

  10. Lexicon and Description of Sui Adjective Intensifiers

    Directory of Open Access Journals (Sweden)

    James N. Stanford

    2007-01-01

    Full Text Available Sui, an indigenous minority language of southwest China, has an elaborate system of adjective intensification. Adjectives are intensified with word-specific, bound morphemes that usually either rhyme with the base or alliterate with the base. Stanford (2007 notes morpho-phonological patterns that suggest reduplication, rhyme, alliteration, The Emergence of the Unmarked (McCarthy & Prince 1994, Yip 2001, identity avoidance, and “Copy But Don’t Repeat” (Kennard 2004. However, the adjective intensifiers defy a simple, fully predictable explanation in such terms; the intensifier lexicon may be best described as “patterned variety,” a case of lexicalized poetry or a poeticized lexicon. Word formation is guided by general patterns, but each specific intensifier may vary within those overall guidelines. Many adjectives have multiple intensifiers that bear subtle semantic and pragmatic distinctions. The current paper serves as a complement to Stanford (2007 by providing a detailed lexicon of the Sui adjective intensifiers for future reference and further analysis. This lexicon is based on the author’s fieldwork and represents the first detailed account of Sui adjective intensifiers for the wider linguistic community.

  11. Streptococcus suis meningitis can require a prolonged treatment course

    Directory of Open Access Journals (Sweden)

    Jean Dejace

    2017-12-01

    Full Text Available We report a case of recrudescent Streptococcus suis meningitis requiring a prolonged treatment course. A few similar cases can be found in the burgeoning literature on what remains a relatively uncommon disease in humans, and these patients should be monitored carefully upon completion of therapy. Keywords: Meningitis, Relapse, Duration, Streptococcus suis

  12. Uptake of benzimidazoles by Trichuris suis in vivo in pigs

    DEFF Research Database (Denmark)

    Hansen, Tina Vicky Alstrup; Friis, Christian; Nejsum, Peter

    2014-01-01

    It is recognized that the clinical efficacy of single dose benzimidazoles (BZs) against the nematode, Trichuris suis of pigs and the closely related Trichuris trichiura in humans is only poor to moderate. Recent in vitro studies have indicated that a low uptake of fenbendazole (FBZ) in T. suis may...

  13. La población con discapacidad en los censos del siglo XX en Costa Rica

    Directory of Open Access Journals (Sweden)

    Mariana Campos Vargas

    2013-01-01

    Full Text Available En este trabajo se logró conocer los criterios de clasificación empleados para definir a la población con discapacidad en los registros censales del siglo XX en Costa Rica y, por consiguiente, una aproximación a las formulaciones conceptuales para referirse al grupo en estudio. Además, se caracterizó a ese segmento poblacional a partir de diferentes variables: sexo, edad, estado civil y distribución geográfica. También se abordaron otros aspectos como fueron: alfabetismo, nivel de educación, ocupación, jefatura de hogar y vivienda. Entre los hallazgos más destacables se encuentra la permanencia, en los registros censales del siglo XX, de un concepto de discapacidad basado en la deficiencia, lo que definió los criterios de clasificación para esa población en esas fuentes. Asimismo se constató, por una parte, la asociación entre discapacidad e inequidad en esa centuria y, por la otra, la fragilidad de los vínculos de parentesco en ese grupo poblacional con respecto al conjunto de hogares de Costa Rica a fines de ese siglo. Las fuentes empleadas en esta investigación fueron los censos de población de Costa Rica del siglo XX. En el procedimiento con los datos, se llevó a cabo un análisis descriptivo de distribución de frecuencias.

  14. Detection and transmission of extracellular fac-tor producing Streptococcus suis serotype 2 strains in pigs

    NARCIS (Netherlands)

    Swildens, B.

    2009-01-01

    DETECTION AND TRANSMISSION OF EXTRACELLULAR FACTOR PRODUCING STREPTOCOCCUS SUIS SEROTYPE 2 STRAINS IN PIGS INTRODUCTION Streptococcus suis (S.suis) has been implicated in the etiology of many diseases among which meningitis in pigs. The virulent extracellular factor-positive strains of S.suis

  15. Safety design of next generation SUI of CANDU stations

    International Nuclear Information System (INIS)

    Nasimi, Elnara; Gabbar, Hossam A.

    2013-01-01

    Highlights: ► Review of current SUI technologies and challenges. ► Propose a new type of SUI detectors. ► Propose a new SUI system architecture and layout. ► Propose implementation procedure for SUI with reduced risks. - Abstract: Due to the age and operating experience of Nuclear Power Plants, equipment ageing and obsolescence has become one of the main challenges that need to be resolved for all systems, structures and components in order to ensure a safe and reliable production of energy. This paper summarizes the research into a methodology for modernization of Start-Up Instrumentation (SUI), both in-core and Control Room equipment, using a new generation of detectors and cables in order to manage obsolescence. The main objective of this research is to develop a new systematic approach to SUI installation/replacement procedure development and optimization. Although some additional features, such as real-time data monitoring and storage/archiving solutions for SUI systems are also examined to take full advantage of today's digital technology, the objectives of this study do not include detailed parametrical studies of detector or system performance. Instead, a number of technological, operational and maintenance issues associated with Start-Up Instrumentation systems at Nuclear Power Plants (NPPs) will be identified and a structured approach for developing a replacement/installation procedure that can be standardized and used across all of the domestic CANDU (Canadian Deuterium Uranium) stations is proposed.

  16. Immune receptors involved in Streptococcus suis recognition by dendritic cells.

    Directory of Open Access Journals (Sweden)

    Marie-Pier Lecours

    Full Text Available Streptococcus suis is an important swine pathogen and an emerging zoonotic agent of septicemia and meningitis. Knowledge on host immune responses towards S. suis, and strategies used by this pathogen for subversion of these responses is scarce. The objective of this study was to identify the immune receptors involved in S. suis recognition by dendritic cells (DCs. Production of cytokines and expression of co-stimulatory molecules by DCs were shown to strongly rely on MyD88-dependent signaling pathways, suggesting that DCs recognize S. suis and become activated mostly through Toll-like receptor (TLR signaling. Supporting this fact, TLR2(-/- DCs were severely impaired in the release of several cytokines and the surface expression of CD86 and MHC-II. The release of IL-12p70 and CXC10, and the expression of CD40 were found to depend on signaling by both TLR2 and TLR9. The release of IL-23 and CXCL1 were partially dependent on NOD2. Finally, despite the fact that MyD88 signaling was crucial for DC activation and maturation, MyD88-dependent pathways were not implicated in S. suis internalization by DCs. This first study on receptors involved in DC activation by S. suis suggests a major involvement of MyD88 signaling pathways, mainly (but not exclusively through TLR2. A multimodal recognition involving a combination of different receptors seems essential for DC effective response to S. suis.

  17. La población con discapacidad en los censos del siglo XX en Costa Rica

    Directory of Open Access Journals (Sweden)

    Mariana Campos Vargas

    2013-07-01

    Full Text Available En este trabajo se logró conocer los criterios de clasificación empleados para definir a la población con discapacidad en los registros censales del siglo XX en Costa Rica y, por consiguiente, una aproximación a las formulaciones conceptuales para referirse al grupo en estudio. Además, se caracterizó a ese segmento poblacional a partir de diferentes variables: sexo, edad, estado civil y distribución geográfica. También se abordaron otros aspectos como fueron: alfabetismo, nivel de educación, ocupación, jefatura de hogar y vivienda. Entre los hallazgos más destacables se encuentra la permanencia, en los registros censales del siglo XX, de un concepto de discapacidad basado en la deficiencia, lo que definió los criterios de clasificación para esa población en esas fuentes. Asimismo se constató, por una parte, la asociación entre discapacidad e inequidad en esa centuria y, por la otra, la fragilidad de los vínculos de parentesco en ese grupo poblacional con respecto al conjunto de hogares de Costa Rica a fines de ese siglo. Las fuentes empleadas en esta investigación fueron los censos de población de Costa Rica del siglo XX. En el procedimiento con los datos, se llevó a cabo un análisis descriptivo de distribución de frecuencias.This research discloses the classification criteria used to define the population with disability in the twentieth century census records in Costa Rica and therefore gives an approximation of the conceptual formulations needed to refer to the examined group. Additionally, a segment of the population has been characterized by sex, age, marital status and geographical distribution. Other aspects like literacy rate, education level, occupation, head of household and housing were also dealt with. The disability concept based on deficiency, which defined the classification criteria for the population in those data sources, is among the most significant findings of this research. Furthermore, it was found

  18. Um panorama da migração internacional a partir do Censo Demográfico de 2010

    Directory of Open Access Journals (Sweden)

    Antônio Tadeu Oliveira

    Full Text Available O artigo apresenta uma análise descritiva dos dados sobre migração internacional provenientes do Censo Demográfico 2010, tanto dos quesitos de imigração, tradicionalmente investigados, quanto dos quesitos inovadores sobre a emigração. Além disso, adere ao debate sobre a pertinência de se classificar o Brasil enquanto País de imigração ou emigração.

  19. In vitro hatching of Trichuris suis eggs

    DEFF Research Database (Denmark)

    Vejzagic, Nermina; Thamsborg, Stig Milan; Kringel, Helene

    2015-01-01

    Eggs of the pig whipworm, Trichuris suis ova (TSO), are currently tested in human clinical trials for their potential immunomodulatory capacity. The biological potency of TSO (egg viability and infectivity) is traditionally assessed in Göttingen minipigs as the establishment of intestinal larvae...... after inoculation with a known number of eggs. To minimize testing in animal models, development of an in vitro egg hatching assay is proposed as a reliable, cost-effective, and a faster alternative to test the egg viability. The present study aimed to investigate the influence of different chemical......, physical, and biological factors on egg hatching. Thus, in a series of experiments and in different combinations, the eggs were stimulated with glass beads, artificial gastric juice, bile salt and trypsin solution, fermentation gut medium, or stimulated with mucosal scrapings from the ileum and the large...

  20. [Streptococcus suis meningitis in a meat factory employee].

    Science.gov (United States)

    de Ceuster, Laura M E; van Dillen, Jeroen J; Wever, Peter C; Rozemeijer, Wouter; Louwerse, Elisabeth S

    2012-01-01

    In the Netherlands, Streptococcus suis is a rare cause of meningitis. Over the past few years, the number of reported cases worldwide has increased. The bacterium is mainly isolated in pigs, but humans can also become infected. At the Emergency Department, a 60-year-old man presented with headache, confusion, fever and nuchal rigidity. He worked at a meat factory. Laboratory testing showed abnormalities linked to bacterial meningitis. S. suis was cultured from blood and cerebrospinal fluid. The patient was treated with dexamethasone, ceftriaxone and later benzylpenicillin intravenously. He recovered well, but had bilateral perceptive hearing loss as a sequela. Particularly people who are in close contact with pigs have an increased risk of S. suis infection. S. suis meningitis can be very severe and lead to serious complications and even death. Rapid diagnosis and adequate treatment are critical. Permanent hearing loss is the most frequent sequela.

  1. Combination Therapy Strategies Against Multiple-Resistant Streptococcus Suis

    Directory of Open Access Journals (Sweden)

    Yang Yu

    2018-05-01

    Full Text Available Streptococcus suis is a major swine pathogen, an emerging zoonotic agent responsible for meningitis, endocarditis and septicaemia followed by deafness in humans. The development of antimicrobial resistance in S. suis increases the risk for therapeutic failure in both animals and humans. In this study, we report the synergism of combination therapy against multi-resistant S. suis isolates from swine. Twelve antibiotic profiles were determined against 11 S. suis strains. To investigate their synergistic/antagonistic activity, checkerboard assay was performed for all the possible combinations. In-vitro killing curves and in-vivo treatment trials were used to confirm the synergistic activity of special combinations against S. suis dominant clones. In this study, 11 S. suis isolates were highly resistant to erythromycin, clindamycin, trimethoprim/sulfamethoxazole, and tetracycline with ratios of 80–100%, and the resistance percentages to enrofloxacin, florfenicol, and spectinomycin were ~50%. The checkerboard data identified two combination regimens, ampicillin plus apramycin and tiamulin plus spectinomycin which gave the greatest level of synergism against the S. suis strains. In-vitro kill-curves showed a bacterial reduction of over 3-logCFU with the use of combination treatments, whilst the application of mono-therapies achieve less than a 2-logCFU cell killing. In-vivo models confirm that administration of these two combinations significantly reduced the number of bacterial cells after 24 h of treatment. In conclusions, the combinations of ampicillin plus apramycin and tiamulin plus spectinomycin showed the greatest synergism and may be potential strategies for treatment of multi-resistant S. suis in animal.

  2. Duplex PCR for differentiation of the vaccine strain Brucella suis S2 and B. suis biovar 1 from other strains of Brucella spp.

    Science.gov (United States)

    Nan, Wenlong; Tan, Pengfei; Wang, Yong; Xu, Zouliang; Mao, Kairong; Peng, Daxin; Chen, Yiping

    2014-09-01

    Immunisation with attenuated Brucella spp. vaccines prevents brucellosis, but may also interfere with diagnosis. In this study, a duplex PCR was developed to distinguish Brucella suis vaccine strain S2 from field strains of B. suis biovar 1 and other Brucella spp. The PCR detected 60 fg genomic DNA of B. suis S2 or biovar 1 field strains and was able to distinguish B. suis S2 and wild-type strains of B. suis biovar 1 among 76 field isolates representing all the common species and biovars, as well as four vaccine strains, of Brucella. Copyright © 2014 Elsevier Ltd. All rights reserved.

  3. Molecular diagnostics of swine infection caused by Mycoplasma suis

    Directory of Open Access Journals (Sweden)

    Potkonjak Aleksandar

    2009-01-01

    Full Text Available The presence of two types of haemoplasm can be established in the swine population. Pathogenic haemoplasm, named Mycoplasma suis (previously called Eperythrozoon suis is the cause of swine eperythrozoonosis or swine ichtheroanaemia. The cause of this disease can also infect humans. The disease has spread all over the world. The most frequent form is latent infection of swine caused by M. suis. The disease is clinically manifest following action by the stress factor. The acute course of the disease is characterized by the occurrence of a febrile condition and ichtheroanaemia. The disease is usually diagnosed based on an epizootiological poll, a clinical examination, and a microscopic examination of a blood smear stained most often according to Giemsa. Contemporary methods of molecular biology have been developed, such as PCR, which are more sensitive and specific in making a diagnosis of swine infection caused by M. suis. In these investigations, the presence of M. suis on pig farms in the Republic of Serbia has been determined using the PCR test. .

  4. Population dynamics of Trichuris suis in trickle-infected pigs.

    Science.gov (United States)

    Nejsum, P; Thamsborg, S M; Petersen, H H; Kringel, H; Fredholm, M; Roepstorff, A

    2009-05-01

    The population dynamics of Trichuris suis in pigs was studied during long-term experimental infections. Twenty-three 10-week-old pigs were inoculated with 5 T. suis eggs/kg/day. Seven, 8, and 8 pigs were necropsied at weeks 4, 8, and 14 post-start of infection (p.i.), respectively. The median numbers of worms in the colon were 538 (min-max: 277-618), 332 (14-1140) and 0 (0-4) at 4, 8, and 14 weeks p.i. respectively, suggesting an increased aggregation of the worms with time and acquisition of nearly sterile immunity. The serum levels of T. suis specific antibodies (IgG1, IgG2 and IgA) peaked at week 8 p.i. By week 14 p.i. the IgG2 and IgA antibody levels remained significantly elevated above the level of week 0. The population dynamics of T. suis trickle infections in pigs is discussed with focus on interpretation of diagnostic and epidemiological data of pigs, the use of pigs as a model for human Trichuris trichiura infections and the novel approach of using T. suis eggs in the treatment of patients with inflammatory bowel disease.

  5. Morphologic, biometric, and isoenzyme characterization of Trichuris suis.

    Science.gov (United States)

    Oliveros, R; Cutillas, C; Arias, P; Guevara, D

    1998-06-01

    Trichuris suis isolates were collected from the cecum of Sus scrofa domestica (pig) and S. s. scrofa (wild boar). Morphology and biometry studies were carried out. Morphology studies showed the existence of typical caudal papillae in males of T. suis from wild boars, but no other difference was observed in the biometric parameters (total length, esophageal length, posterior-portion body length, and spicular length) of T. suis isolated from either host. Individual extracts were subjected to malate dehydrogenase (MDH), malic enzyme (ME), glucose 6-phosphate dehydrogenase (G6PD), lactate dehydrogenase (LDH), and superoxide dismutase (SOD) isoenzyme analysis following starch-gel electrophoresis, and the isoenzyme patterns were compared with those obtained from other species of trichurids. MDH, ME, G6PD, LDH, and SOD isoenzyme patterns were identical for T. suis from both hosts. MDH isoenzyme patterns were characterized by the presence of one cathodic isoenzyme. ME, G6PD, and LDH isoenzyme patterns indicated the presence of three phenotypes, whereas the SOD isoenzyme pattern showed only one phenotype characterized by the existence of two (anodic and cathodic) bands. Different LDH and SOD isoenzyme patterns observed for T. suis, T. ovis, and T. skrjabini confirm once more that isoenzyme patterns have potential as a diagnostic tool for differentiation of different species of Trichuris.

  6. Research on ethics in two large Human Biomonitoring projects ECNIS and NewGeneris: a bottom up approach

    Directory of Open Access Journals (Sweden)

    Casteleyn Ludwine

    2008-01-01

    . This will not only increase the possibilities for comparison between data generated but may also allow for more equality in the protection of the rights of European citizens and establish trustful relationships between science and society, based on firmly rooted ethical values within the EU legislative framework. These considerations outline part of the research on legal, socio-ethical and communication aspects of HBM within the scope of ECNIS (NoE and NewGeneris (IP.

  7. Research on ethics in two large Human Biomonitoring projects ECNIS and NewGeneris: a bottom up approach.

    Science.gov (United States)

    Dumez, Birgit; Van Damme, Karel; Casteleyn, Ludwine

    2008-06-05

    the possibilities for comparison between data generated but may also allow for more equality in the protection of the rights of European citizens and establish trustful relationships between science and society, based on firmly rooted ethical values within the EU legislative framework.These considerations outline part of the research on legal, socio-ethical and communication aspects of HBM within the scope of ECNIS (NoE) and NewGeneris (IP).

  8. Streptococcus suis Meningitis: A Systematic Review and Meta-analysis

    Science.gov (United States)

    van Samkar, Anusha; Brouwer, Matthijs C.; Schultsz, Constance; van der Ende, Arie; van de Beek, Diederik

    2015-01-01

    Background Streptococcus suis is the most common cause of meningitis in pork consuming and pig rearing countries in South-East Asia. We performed a systematic review of studies on S. suis meningitis to define the clinical characteristics, predisposing factors and outcome. Methodology Studies published between January 1, 1980 and August 1, 2015 were identified from main literature databases and reference lists. Studies were included if they were written in West-European languages and described at least 5 adult patients with S. suis meningitis in whom at least one clinical characteristic was described. Findings We identified 913 patients with S. suis meningitis included in 24 studies between 1980 and 2015. The mean age was 49 years and 581 of 711 patients were male (82%). Exposure to pigs or pork was present in 395 of 648 patients (61%) while other predisposing factors were less common. 514 of 528 patients presented with fever (97%), 429 of 451 with headache (95%), 462 of 496 with neck stiffness (93%) and 78 of 384 patients (20%) had a skin injury in the presence of pig/pork contact. The case fatality rate was 2.9% and hearing loss was a common sequel occurring in 259 of 489 patients (53%). Treatment included dexamethasone in 157 of 300 (52%) of patients and was associated with reduced hearing loss in S. suis meningitis patients included in a randomized controlled trial. Conclusion S. suis meningitis has a clear association with pig and pork contact. Mortality is low, but hearing loss occurs frequently. Dexamethasone was shown to reduce hearing loss. PMID:26505485

  9. Streptococcus suis Meningitis: First Case Reported in Quebec

    Directory of Open Access Journals (Sweden)

    Sophie Michaud

    1996-01-01

    Full Text Available Very few Streptococcus suis infections in humans have been reported in Canada, although the condition is frequent in pigs. Meningitis, often accompanied by severe hearing loss, is the most common clinical manifestation. The disease is an occupational illness affecting persons in contact with pigs and may be underdiagnosed because of misidentification of the responsible bacterium. Since Quebec is the leading province for swine production in Canada, physicians and microbiologists should be aware of this infection, especially when a streptococcal meningitis is diagnosed in swine workers. The first case of S suis type 2 meningitis reported in Quebec is described.

  10. Epidemiological relationship of human and swine Streptococcus suis isolates.

    Science.gov (United States)

    Tarradas, C; Luque, I; de Andrés, D; Abdel-Aziz Shahein, Y E; Pons, P; González, F; Borge, C; Perea, A

    2001-06-01

    Two cases of meningitis due to Streptococcus suis in humans are reported here. A butcher and an abattoir worker were referred to a health centre in Castellón (Spain) with fever and symptoms of meningitis. After adequate treatment, a slight hipoacusia persisted as sequelae in both cases. Colonies of S. suis group R, serotype 2 and phenotype MRP+EF+ were isolated from cerebroespinal fluid. Epidemiological studies showed that both workers had in common the handling of pork meat of slaughtered healthy pigs from three closed farms. A study of the tonsils from apparently healthy, slaughtered pigs was carried out. A total of 234 tonsillar samples were obtained and 81 strains of S. suis were isolated from them. Serotype 2 appeared to be the most frequent (50.6%), and the analysis for phenotype showed a high percentage of tonsillar strains with the phenotype MRP+EF+ (35.9%). The humans and 28 tonsillar swine strains showed a similar profile (S. suis group R, serotype 2 and phenotype MRP+EF+). A total of 26 of the swine isolates were analysed by ribotyping using EcoRI. The human strains showed the same six-band hybridization pattern that shared five bands with the pattern most frequently shown by most of the tonsillar N. suis group R, serotype 2 and phenotype MRP+EF+ strains, differing only in the lightest, faintest band which was slightly less anodical in human (> or = 1.8 kb) than in swine (approximately 1.8 kb). From these results, both groups of strains, humans and porcine, showed differences; how can these differences in the pattern of ribotyping be explained if they should have the same origin? Is it possible that they have undergone an adaptation to the new host or perhaps the modification is due to other unknown causes? Further studies in this area are required in order to answer these questions.

  11. La industria del Gran La Plata según el último Censo Nacional Económico

    Directory of Open Access Journals (Sweden)

    Diego Arturi

    2012-12-01

    Full Text Available El artículo tiene como objetivo caracterizar el sector industrial del Gran La Plata en base a información del último Censo Nacional Económico (CNE 2004/2005. En una primer parte se identifican las principales características del sector industrial a escala nacional en los períodos neoliberal y posconvertibilidad, a partir de bibliografía especializada y análisis de series estadísticas e informes técnicos. En una segunda parte se analiza el sector industrial del Gran La Plata en base a entrevistas a informantes calificados y a los datos censales según cantidad de establecimientos, puestos de trabajo ocupados, rama de actividad, tamaño de los locales industriales, productividad y especialización industrial de los municipios; asimismo se realiza una aproximación a niveles de intensidad tecnológica por rama. A partir de este análisis se pueden destacar las siguientes conclusiones: predominio de las micro y pequeñas empresas, productividad superior al promedio nacional, escaso aporte de las ramas de alta intensidad tecnológica y ausencia de especialización que impide definir un perfil industrial marcado.

  12. La escolarización elemental en España según el censo de Godoy (1797

    Directory of Open Access Journals (Sweden)

    Francisco Javier LASPALAS PÉREZ

    2010-03-01

    Full Text Available ¿Cuántos municipios españoles tenían escuela hace dos o tres siglos? ¿Qué porcentaje de niños acudía en nuestro país a las escuelas de primeras letras durante el Antiguo Régimen? No resulta fácil contestar a estas preguntas, pues apenas disponemos de fuentes en las que apoyarnos para responderlas, o no se han descubierto todavía. Junto con el Catastro de Ensenada (1751, en el que se basan algunas de las investigaciones más interesantes y recientes sobre el sistema escolar de la España del siglo XVIII, el Censo de Godoy (17971 es, sin duda, uno de los documentos más valiosos para el estudio del tema que nos ocupa, puesto que incluye un recuento por provincias del número de alumnos y de escuelas primarias que existían en el país.

  13. La mortalidad en la infancia durante la Guerra Civil. Impacto territorial estimado a partir del Censo de 1940

    Directory of Open Access Journals (Sweden)

    Fernando Gil Alonso

    2009-01-01

    Full Text Available En este artículo se utilizan las preguntas del Censo de 1940 sobre la fecundidad retrospectiva ?hijos nacidos vivos e hijos supervivientes en el momento censal? de las mujeres casadas al menos una vez para estimar los niveles de supervivencia de sus descendientes y, por tanto, de mortalidad en la infancia (hasta los 15 años, según un método propuesto por W. Brass. Los resultados muestran que la Guerra Civil empeoró los niveles de supervivencia en la infancia en prácticamente todas las provincias españolas, pero tuvo un impacto diferencial muy contrastado. Comparando los niveles de supervivencia antes y durante el conflicto bélico, se obtiene que fueron las provincias meridionales y levantinas las más afectadas, mientras que en Cataluña y la Meseta septentrional los niveles de mortalidad en la infancia parecen haber empeorado menos, pese a que mostraban posiciones extremas respecto a los niveles de supervivencia antes de la guerra. De esto se deduce que, a pesar de que debiera existir una cierta relación entre el nivel de desarrollo demográfico y la vulnerabilidad que muestran las provincias ante una coyuntura desfavorable, tales características no parecen jugar un papel significativo como factor explicativo de los niveles de supervivencia durante el conflicto bélico.

  14. Meningitis por Streptococcus suis en un paciente inmunocompetente Streptococcus suis meningitis in an immunocompetent patient

    Directory of Open Access Journals (Sweden)

    A. Nagel

    2008-09-01

    Full Text Available Se describe un caso de meningitis por Streptococcus suis en un paciente inmunocompetente. Presentaba astenia, debilidad generalizada, fiebre (39 °C, vómitos, deterioro del sensorio y desorientación témporo-espacial. Los cultivos de sangre (2/2 y de líquido cefalorraquídeo fueron positivos. La identificación preliminar se realizó utilizando las pruebas bioquímicas convencionales y fue completada en el Servicio Bacteriología Especial del INEI-ANLIS "Dr. Carlos G. Malbrán". Se comenzó el tratamiento con ampicilina y ceftriaxona. El microorganismo aislado demostró sensibilidad a ampicilina, cefotaxima y vancomicina. El paciente evolucionó favorablemente, pero se comprobó leve hipoacusia. Reingresó a los 4 meses con marcha atáxica, anacusia en oído izquierdo e hipoacusia en oído derecho. Continúa con seguimiento neurológico y audiométrico. Retrospectivamente se constató el contacto del paciente con cerdos. Se destaca la importancia de la anamnesis para alertar la sospecha de este agente etiológico en meningitis y bacteriemias.A case of Streptococcus suis meningitis is described in an immunocompetent patient presenting asthenia, general weakness, fever, vomiting, sensory deterioration and temporospatial disorder. The cerebrospinal fluid and two blood cultures (2/2 bottles were positive. The isolate was preliminary identified by conventional biochemical tests, and the identification was completed at the Special Bacteriology Service of INEI-ANLIS "Dr. Carlos G. Malbrán". Ampicillin and ceftriaxone treatment was initiated. The isolate was susceptible to ampicillin, cefotaxime and vancomycin. The patient experienced a good outcome but suffered hearing loss. However, after four months he returned with walking ataxia, deafness in his left ear, and hearing loss in the right ear. The patient’s retrospective exposure to pigs had been verified. It is important to evaluate predisposing and epidemiologic factors in order to alert about

  15. La enumeración de la soltería femenina en los censos de población: sesgo y propuesta de corrección

    Science.gov (United States)

    McCaa, Robert; Esteve, Albert; Garcia, Joan

    2013-01-01

    Esta investigación tiene como objetivo investigar el efecto que la disolución de las uniones consensuales tiene en los niveles de soltería que proporciona el Censo de Población, niveles derivados de la variable estado civil. Para ello comparamos los datos censales con los de la Encuestas de Demografía y Salud (de ahora en adelante DHS) en aquellos países y años para los que disponemos de ambas fuentes en el mismo año o años adyacentes (Bolivia, Brasil, Colombia y Perú). Los resultados muestran claramente que las proporciones de nunca unidas derivadas de la variable censal ’Estado civil’ son sistemáticamente más elevadas que las estimadas a partir de las DHS. La razón de esta sobreestimación obedece al hecho de que personas que estuvieron en unión libre en el pasado se declaran solteras en el momento del Censo. La elevada proporción de mujeres solteras que tienen hijos según el Censo es una prueba de ello y a su vez una solución efectiva para corregir el sesgo. PMID:25593515

  16. Whole genome investigation of a divergent clade of the pathogen Streptococcus suis

    Directory of Open Access Journals (Sweden)

    Abiyad eBaig

    2015-11-01

    Full Text Available Streptococcus suis is a major porcine and zoonotic pathogen responsible for significant economic losses in the pig industry and an increasing number of human cases. Multiple isolates of S. suis show marked genomic diversity. Here we report the analysis of whole genome sequences of nine pig isolates that caused disease typical of S. suis and had phenotypic characteristics of S. suis, but their genomes were divergent from those of many other S. suis isolates. Comparison of protein sequences predicted from divergent genomes with those from normal S. suis reduced the size of core genome from 793 to only 397 genes. Divergence was clear if phylogenetic analysis was performed on reduced core genes and MLST alleles. Phylogenies based on certain other genes (16S rRNA, sodA, recN and cpn60 did not show divergence for all isolates, suggesting recombination between some divergent isolates with normal S. suis for these genes. Indeed, there is evidence of recent recombination between the divergent and normal S. suis genomes for 249 of 397 core genes. In addition, phylogenetic analysis based on the 16S rRNA gene and 132 genes that were conserved between the divergent isolates and representatives of the broader Streptococcus genus showed that divergent isolates were more closely related to S. suis. Six out of nine divergent isolates possessed a S. suis-like capsule region with variation in capsular gene sequences but the remaining three did not have a discrete capsule locus. The majority (40/70, of virulence-associated genes in normal S. suis were present in the divergent genomes. Overall, the divergent isolates extend the current diversity of S. suis species but the phenotypic similarities and the large amount of gene exchange with normal S. suis gives insufficient evidence to assign these isolates to a new species or subspecies. Further sampling and whole genome analysis of more isolates is warranted to understand the diversity of the species.

  17. Bulky DNA Adducts in Cord Blood, Maternal Fruit-and-Vegetable Consumption, and Birth Weight in a European Mother-Child Study (NewGeneris)

    DEFF Research Database (Denmark)

    Pedersen, Marie; Schoket, Bernadette; Godschalk, Roger W

    2013-01-01

    , Greece, Norway, and Spain were recruited in 2006-2010. Adduct levels were measured by the 32P-postlabeling technique in white blood cells from 229 mothers and 612 newborns. Maternal diet was examined through questionnaires.Results: Adduct levels in maternal and cord blood samples were similar...... versus lowest tertile of adducts. The negative association with birth weight was limited to births in Norway, Denmark, and England, the countries with the lowest adduct levels, and was more pronounced in births to mothers with low intake of fruits and vegetables (-248 g; 95% CI: -405, -92 g) compared......, Kleinjans JC, Segerbäck D, Kogevinas M. 2013. Bulky DNA adducts in cord blood, maternal fruit-and-vegetable consumption, and birth weight in a European mother-child study (NewGeneris). Environ Health Perspect 121:1200-1206; http://dx.doi.org/10.1289/ehp.1206333....

  18. Censo dos pacientes internados em uma instituição asilar no Estado do Rio de Janeiro: dados preliminares

    Directory of Open Access Journals (Sweden)

    Gomes Maria Paula Cerqueira

    2002-01-01

    Full Text Available O Estado do Rio de Janeiro concentra uma das maiores redes de hospitais psiquiátricos do país, sendo um deles a Casa de Saúde Dr. Eiras/Paracambi (CSDE-P. A atual Assessoria de Saúde Mental da Secretaria de Estado de Saude do Estado do Rio de Janeiro, realizou em fins de 2000, o Censo Clínico e Psicossocial dos Pacientes Internados na CSDE-P. Encontrou uma população de 1494 indivíduos, com predomínio de homens (53,4%, solteiros (81,6%, faixa etária produtiva (66,4% e baixa escolaridade (11,5% com primeiro grau completo ou mais. A maioria tinha um tempo de internação superior/igual a 2 anos (77,6%, recebiam visitas de familiares (60,5% mas não saíam de licença (73,8%. Os diagnósticos predominantes foram as esquizofrenias (53,6% e o retardo mental (26,4%. A principal abordagem terapêutica foi a psiquiátrica (84,1%. Realizavam atividades sistemáticas durante o dia 13,3% dos pacientes, embora cerca de 36% apresentassem bons indicativos de condições de autonomia. A CSDE-P é um macro hospital, onde a maioria dos pacientes encontra-se desterritorializada e com laços sociais precários, exigindo a construção de estratégias de cuidados que levem em conta essa particularidade.

  19. The Complete Genome of Brucella Suis 019 Provides Insights on Cross-Species Infection

    Directory of Open Access Journals (Sweden)

    Yuanzhi Wang

    2016-01-01

    Full Text Available Brucella species are the most important zoonotic pathogens worldwide and cause considerable harm to humans and animals. In this study, we presented the complete genome of B. suis 019 isolated from sheep (ovine with epididymitis. B. suis 019 has a rough phenotype and can infect sheep, rhesus monkeys and possibly humans. The comparative genome analysis demonstrated that B. suis 019 is closest to the vaccine strain B. suis bv. 1 str. S2. Further analysis associated the rsh gene to the pathogenicity of B. suis 019, and the WbkA gene to the rough phenotype of B. suis 019. The 019 complete genome data was deposited in the GenBank database with ID PRJNA308608.

  20. Clearance of Streptococcus suis in Stomach Contents of Differently Fed Growing Pigs

    OpenAIRE

    Franziska Warneboldt; Saara J. Sander; Andreas Beineke; Peter Valentin-Weigand; Josef Kamphues; Christoph Georg Baums

    2016-01-01

    Streptococcus (S.) suis translocates across the intestinal barrier of piglets after intraintestinal application. Based on these findings, an oro-gastrointestinal infection route has been proposed. Thus, the objective of this study was to investigate the survival of S. suis in the porcine stomach. Whereas surviving bacteria of S. suis serotypes 2 and 9 were not detectable after 60 min of incubation in stomach contents with a comparatively high gastric pH of 5 due to feeding of fine pellets, th...

  1. Streptococcus suis Interactions with the Murine Macrophage Cell Line J774: Adhesion and Cytotoxicity

    OpenAIRE

    Segura, Mariela; Gottschalk, Marcelo

    2002-01-01

    Streptococcus suis capsular type 2 is an important etiological agent of swine meningitis, and it is also a zoonotic agent. Since one hypothesis of the pathogenesis of S. suis infection is that bacteria enter the bloodstream and invade the meninges and other tissues in close association with mononuclear phagocytes, the objective of the present study was to evaluate the capacity of S. suis type 2 to adhere to macrophages. An enzyme-linked immunosorbent assay technique was standardized to simply...

  2. A trajetória do aprendizado tecnológico nos censos demográficos no Brasil

    Directory of Open Access Journals (Sweden)

    Paulo Ferreira Vilarinho

    Full Text Available O estudo analisa o desenvolvimento tecnológico do Instituto Brasileiro de Geografia e Estatística - IBGE, focalizando os projetos censitários, desde sua origem até o advento do Censo Demográfico 2000, tendo por principais fundamentos teóricos os conceitos da acumulação tecnológica (BELL; PAVITT, 1993, associados às competências dinâmicas (TEECE; PISANO; SCHUEN, 1990 e à visão de inovação e aprendizado (DOSI, 1988. A análise dos dados aplica a estrutura analítica de aprendizado e acumulação de competências tecnológicas (FIGUEIREDO, 2001 em níveis gradativos de dificuldade e complexidade, classificando os recursos geradores de inovação e socialização de conhecimentos e seus efeitos nos processos organizacionais. Os dados da pesquisa foram coletados no ano de 2002, por meio de pesquisa documental e entrevistas com funcionários das diretorias técnicas do IBGE. O estudo demonstra que o órgão demorou 50 anos para evoluir de um nível básico de experimentação tecnológica, assimilada de países desenvolvidos, até renovar sua tecnologia nos anos 1990, iniciando um processo contínuo e autóctone de geração de conhecimento. Em 2000, logrou alcançar um nível internacionalmente avançado de competência tecnológica passível de exportação. Ao final, o estudo avalia que a visão compartimentada dos órgãos públicos tende a criar "ilhas de especialização" e a não otimizar o uso de tecnologias recém-defasadas.

  3. Desarrollo de una base de datos integrada de Censo y encuesta mediante el uso de elementos de inteligencia de negocios y SIG

    OpenAIRE

    Cornejo,Robert; Navarrete,Mónica; Valdivia,Ricardo; Aroca,Patricio; Aracena,Sebastián

    2014-01-01

    En este trabajo se plantea el desarrollo de una solución de Inteligencia de Negocios y Sistemas de Información Geográfica (SIG) para gestionar integradamente los datos generados por el Censo Nacional de personas, hogares y viviendas y la Encuesta de Clasificación Económica y Social (CASEN) como una alternativa a los métodos actuales de estimación en área pequeña (SAE) utilizados para obtener indicadores desagregados de bienestar y que estiman, por ejemplo, el ingreso a partir de los atributos...

  4. ¿Por qué emigran los colombianos? Un análisis departamental basado en el Censo de 2005

    Directory of Open Access Journals (Sweden)

    David Khoudour-Castéras

    2007-07-01

    Full Text Available El Censo de 2005 ofrece, por vez primera, información detallada de los flujos migratorios colombianos. Este artículo analiza la información de los colombianos en el exterior y examina las principales causas de la emigración. Calcula la tasa de emigración por departamento y presenta un estudio econométrico con variables estructurales, coyunturales y relacionadas con la violencia. Concluye que las personas menos pobres y mejor preparadas de los departamentos con mayores dificultades socioeconómicas tienen mayor propensión a emigrar.

  5. Evaluation of Galleria mellonella larvae for studying the virulence of Streptococcus suis

    NARCIS (Netherlands)

    Velikova, Nadya; Kavanagh, Kevin; Wells, Jerry M.

    2016-01-01

    Background: Streptococcus suis is an encapsulated Gram-positive bacterium and the leading cause of sepsis and meningitis in young pigs, resulting in considerable economic losses in the porcine industry. S. suis is considered an emerging zoonotic agent with increasing numbers of human cases over the

  6. Isolation, Characterization and Biological Properties of Membrane Vesicles Produced by the Swine Pathogen Streptococcus suis.

    Directory of Open Access Journals (Sweden)

    Bruno Haas

    Full Text Available Streptococcus suis, more particularly serotype 2, is a major swine pathogen and an emerging zoonotic agent worldwide that mainly causes meningitis, septicemia, endocarditis, and pneumonia. Although several potential virulence factors produced by S. suis have been identified in the last decade, the pathogenesis of S. suis infections is still not fully understood. In the present study, we showed that S. suis produces membrane vesicles (MVs that range in diameter from 13 to 130 nm and that appear to be coated by capsular material. A proteomic analysis of the MVs revealed that they contain 46 proteins, 9 of which are considered as proven or suspected virulence factors. Biological assays confirmed that S. suis MVs possess active subtilisin-like protease (SspA and DNase (SsnA. S. suis MVs degraded neutrophil extracellular traps, a property that may contribute to the ability of the bacterium to escape the host defense response. MVs also activated the nuclear factor-kappa B (NF-κB signaling pathway in both monocytes and macrophages, inducing the secretion of pro-inflammatory cytokines, which may in turn contribute to increase the permeability of the blood brain barrier. The present study brought evidence that S. suis MVs may play a role as a virulence factor in the pathogenesis of S. suis infections, and given their composition be an excellent candidate for vaccine development.

  7. Host-pathogen Interaction at the Intestinal Mucosa Correlates With Zoonotic Potential of Streptococcus suis

    DEFF Research Database (Denmark)

    Ferrando, Maria Laura; de Greeff, Astrid; van Rooijen, Willemien J. M.

    2015-01-01

    Background. Streptococcus suis has emerged as an important cause of bacterial meningitis in adults. The ingestion of undercooked pork is a risk factor for human S. suis serotype 2 (SS2) infection. Here we provide experimental evidence indicating that the gastrointestinal tract is an entry site of...... be considered a food-borne pathogen. S. suis interaction with human and pig IEC correlates with S. suis serotype and genotype, which can explain the zoonotic potential of SS2....... of SS2 infection. Methods. We developed a noninvasive in vivo model to study oral SS2 infection in piglets. We compared in vitro interaction of S. suis with human and porcine intestinal epithelial cells (IEC). Results. Two out of 15 piglets showed clinical symptoms compatible with S. suis infection 24......Background. Streptococcus suis has emerged as an important cause of bacterial meningitis in adults. The ingestion of undercooked pork is a risk factor for human S. suis serotype 2 (SS2) infection. Here we provide experimental evidence indicating that the gastrointestinal tract is an entry site...

  8. Establishment of a Cre recombinase based mutagenesis protocol for markerless gene deletion in Streptococcus suis.

    Science.gov (United States)

    Koczula, A; Willenborg, J; Bertram, R; Takamatsu, D; Valentin-Weigand, P; Goethe, R

    2014-12-01

    The lack of knowledge about pathogenicity mechanisms of Streptococcus (S.) suis is, at least partially, attributed to limited methods for its genetic manipulation. Here, we established a Cre-lox based recombination system for markerless gene deletions in S. suis serotype 2 with high selective pressure and without undesired side effects. Copyright © 2014 Elsevier B.V. All rights reserved.

  9. Enzymatic, immunological and phylogenetic characterization of Brucella suis urease

    Directory of Open Access Journals (Sweden)

    Sriranganathan Nammalwar

    2008-07-01

    Full Text Available Abstract Background The sequenced genomes of the Brucella spp. have two urease operons, ure-1 and ure-2, but there is evidence that only one is responsible for encoding an active urease. The present work describes the purification and the enzymatic and phylogenomic characterization of urease from Brucella suis strain 1330. Additionally, the urease reactivity of sera from patients diagnosed with brucellosis was examined. Results Urease encoded by the ure-1 operon of Brucella suis strain 1330 was purified to homogeneity using ion exchange and hydrophobic interaction chromatographies. The urease was purified 51-fold with a recovery of 12% of the enzyme activity and 0.24% of the total protein. The enzyme had an isoelectric point of 5, and showed optimal activity at pH 7.0 and 28–35°C. The purified enzyme exhibited a Michaelis-Menten saturation kinetics with a Km of 5.60 ± 0.69 mM. Hydroxyurea and thiourea are competitive inhibitors of the enzyme with Ki of 1.04 ± 0.31 mM and 26.12 ± 2.30 mM, respectively. Acetohydroxamic acid also inhibits the enzyme in a competitive way. The molecular weight estimated for the native enzyme was between 130–135 kDa by gel filtration chromatography and 157 ± 7 kDa using 5–10% polyacrylamide gradient non-denaturing gel. Only three subunits in SDS-PAGE were identified: two small subunits of 14,000 Da and 15,500 Da, and a major subunit of 66,000 Da. The amino terminal sequence of the purified large subunit corresponded to the predicted amino acid sequence encoded by ureC1. The UreC1 subunit was recognized by sera from patients with acute and chronic brucellosis. By phylogenetic and cluster structure analyses, ureC1 was related to the ureC typically present in the Rhizobiales; in contrast, the ureC2 encoded in the ure-2 operon is more related to distant species. Conclusion We have for the first time purified and characterized an active urease from B. suis. The enzyme was characterized at the kinetic

  10. Las organizaciones cooperativas en el agro argentino: apuntes metodológicos para su estudio en base al Censo Nacional Agropecuario de 1937

    Directory of Open Access Journals (Sweden)

    Carlos A. Makler

    2006-01-01

    Full Text Available Este trabajo presenta un análisis del movimiento cooperativo en el agro pampeano y en el resto del país, tal como fuera retratado por el Censo Nacional Agropecuario del año 1937. Tiene por objetivo aportar herramientas metodológicas para el conocimiento del grado de arraigo del cooperativismo agropecuario entre los productores agrarios a partir del uso de índices de asociación, adhesión e integración. Finalmente se concluye del estudio estadístico de esos indicadores que la doctrina cooperativa jugó un papel muy relativo en la estructuración de las opciones económicas efectivas de bases sociales del movimiento.This work presents an analysis of the cooperative movement in the pampeano's agro and the country, as was drown by the Censo Nacional Agropeacuario in 1937. This article has the objective of give some metodological tools to the knowledge of the grade of settlement of agropecuarian cooperativism between the agrarian producers from the use of association, adhesion and integration indexes. Finally we conclude from the stadistic study of those indicatoris that the cooperative doctrine played a very relative place in the structuration of efective economic options of the social bases of the movement.

  11. Contribuciones del Censo de Población y Viviendas 2012 al desarrollo socioeconómico del municipio Mayarí, Holguín, Cuba

    Directory of Open Access Journals (Sweden)

    Rafael Ángel Salazar Martínez

    2014-06-01

    Full Text Available El presente trabajo establece las coordenadas para la utilización eficiente de los resultados que arroje el Censo del Población y Viviendas del 2012, en función del desarrollo socioeconómico del municipio Mayarí, territorio que implementa desde ese mismo año un Programa Integral de Desarrollo Municipal (PIDM, cuya significación estratégica radica en el hecho de constituir la experiencia piloto a nivel nacional, para su generalización hacia otros municipios del país, respondiendo a los Lineamientos de la Política Económica y Social del Partido y la Revolución, en lo referente al desarrollo de proyectos locales. En este contexto, resulta de vital importancia el manejo y acceso a fuentes de información confiables y pertinentes, como las que ofrece el Censo de Población y Viviendas, que se traduzca luego en una mayor consistencia a la hora de tomar decisiones y gestionar todos los procesos que intervienen en la ejecución de este Programa.

  12. Reseña: 1er Censo Nacional de Psicología -Uruguay 2014-. Perfil sociodemográfico, formación y desempeño profesional.

    Directory of Open Access Journals (Sweden)

    Jorge Diego Suárez

    2015-05-01

    Full Text Available La publicación que se reseña es un informe publicado en febrero de 2015, que presenta resultados de carácter descriptivo de los datos recabados en el 1er Censo Nacional de Psicología de Uruguay, realizado entre 14 y 31 de julio de 2014 por el Ministerio de Salud Pública y la Facultad de Psicología de la Universidad de la República. Dicho informe, se estructura en un capít ulo introductorio y tres capítulos centrales que reflejan las dimensiones indagadas en el censo, a saber: Perfil Sociodemográfico, Formación y Actuación Profesional y Laboral; más dos Anexos, uno con el Cuestionario Aplicado en el censo y otro con los Datos y Fuentes que delimitaron el universo censal.

  13. Inorganic pyrophosphatase in uncultivable hemotrophic mycoplasmas: identification and properties of the enzyme from Mycoplasma suis

    Directory of Open Access Journals (Sweden)

    Wittenbrink Max M

    2010-07-01

    Full Text Available Abstract Background Mycoplasma suis belongs to a group of highly specialized hemotrophic bacteria that attach to the surface of host erythrocytes. Hemotrophic mycoplasmas are uncultivable and the genomes are not sequenced so far. Therefore, there is a need for the clarification of essential metabolic pathways which could be crucial barriers for the establishment of an in vitro cultivation system for these veterinary significant bacteria. Inorganic pyrophosphatases (PPase are important enzymes that catalyze the hydrolysis of inorganic pyrophosphate PPi to inorganic phosphate Pi. PPases are essential and ubiquitous metal-dependent enzymes providing a thermodynamic pull for many biosynthetic reactions. Here, we describe the identification, recombinant production and characterization of the soluble (sPPase of Mycoplasma suis. Results Screening of genomic M. suis libraries was used to identify a gene encoding the M. suis inorganic pyrophosphatase (sPPase. The M. suis sPPase consists of 164 amino acids with a molecular mass of 20 kDa. The highest identity of 63.7% was found to the M. penetrans sPPase. The typical 13 active site residues as well as the cation binding signature could be also identified in the M. suis sPPase. The activity of the M. suis enzyme was strongly dependent on Mg2+ and significantly lower in the presence of Mn2+ and Zn2+. Addition of Ca2+ and EDTA inhibited the M. suis sPPase activity. These characteristics confirmed the affiliation of the M. suis PPase to family I soluble PPases. The highest activity was determined at pH 9.0. In M. suis the sPPase builds tetramers of 80 kDa which were detected by convalescent sera from experimentally M. suis infected pigs. Conclusion The identification and characterization of the sPPase of M. suis is an additional step towards the clarification of the metabolism of hemotrophic mycoplasmas and, thus, important for the establishment of an in vitro cultivation system. As an antigenic and conserved

  14. Clearance of Streptococcus suis in Stomach Contents of Differently Fed Growing Pigs

    Directory of Open Access Journals (Sweden)

    Franziska Warneboldt

    2016-08-01

    Full Text Available Streptococcus (S. suis translocates across the intestinal barrier of piglets after intraintestinal application. Based on these findings, an oro-gastrointestinal infection route has been proposed. Thus, the objective of this study was to investigate the survival of S. suis in the porcine stomach. Whereas surviving bacteria of S. suis serotypes 2 and 9 were not detectable after 60 min of incubation in stomach contents with a comparatively high gastric pH of 5 due to feeding of fine pellets, the number of Salmonella Derby bacteria increased under these conditions. Further experiments confirmed the clearance of S. suis serotypes 2 and 9 within 30 min in stomach contents with a pH of 4.7 independently of the bacterial growth phase. Finally, an oral infection experiment was conducted, feeding each of 18 piglets a diet mixed with 1010 CFU of S. suis serotype 2 or 9. Thorough bacteriological screenings of various mesenteric-intestinal lymph nodes and internal organs after different times of exposure did not lead to any detection of the orally applied challenge strains. In conclusion, the porcine stomach constitutes a very efficient barrier against oro-gastrointenstinal S. suis infections. Conditions leading to the passage of S. suis through the stomach remain to be identified.

  15. First human case report of sepsis due to infection with Streptococcus suis serotype 31 in Thailand.

    Science.gov (United States)

    Hatrongjit, Rujirat; Kerdsin, Anusak; Gottschalk, Marcelo; Takeuchi, Dan; Hamada, Shigeyuki; Oishi, Kazunori; Akeda, Yukihiro

    2015-09-30

    Streptococcus suis is a zoonotic pathogen that causes invasive infections in humans and pigs. It has been reported that S. suis infection in humans is mostly caused by serotype 2. However, human cases caused by other serotypes have rarely been reported. This is the first report of a human case of infection with S. suis serotype 31 in Thailand. A 55-year-old male alcohol misuser with liver cirrhosis was admitted with sepsis to a hospital in the Central Region of Thailand. He had consumed a homemade, raw pork product prior to the onset of illness. He was alive after treatment with ceftriaxone and no complication occurred. An isolate from blood culture at the hospital was suspected as viridans group Streptococcus. It was confirmed at a reference laboratory as S. suis serotype 31 by biochemical tests, 16S rDNA sequencing, and multiplex polymerase chain reaction for serotyping, but it was untypable by the co-agglutination test with antisera against recognized S. suis serotypes, suggesting loss of capsular material. The absence of a capsule was confirmed by transmission electron microscopy. The isolate was confirmed to be sequence type 221, with 13 putative virulence genes that are usually found in serotype 2 strains. We should be aware of the emergence of S. suis infections caused by uncommon serotypes in patients with predisposing conditions. Laboratory capacity to identify S. suis in the hospital is needed in developing countries, which can contribute to enhanced surveillance, epidemiological control, and prevention strategies in the prevalent area.

  16. Effect of spatial separation of pigs on spread of Streptococcus suis serotype 9.

    Directory of Open Access Journals (Sweden)

    Niels Dekker

    Full Text Available The spread of an infectious agent in a population can be reduced by interfering in the infectiousness or susceptibility of individuals, and/or in their contact structure. The aim of this study was to quantify the effect of prevention of direct contact between infectious and susceptible pigs on the transmission of Streptococcus suis (S. suis. In three replicate experiments, S. suis-free pigs were housed in boxes either in pairs (25 pairs or alone (15 pigs. The distance between the boxes was ±1 m. At 7 weeks of age, one pig of each pair was inoculated intranasally with S. suis serotype 9; the other pigs were exposed to S. suis by either direct (pairs or indirect contact (individually housed pigs. Tonsillar brush and saliva swab samples from all pigs were collected regularly for 4 weeks post inoculation to monitor colonization with S. suis. All inoculated pigs became infected, and their pen mates became colonized within 2 days. Thirteen indirectly exposed pigs became positive within 7-25 days after exposure. The rate of direct transmission βdir was estimated to be 3.58 per pig per day (95% CI: 2.29-5.60. The rate of indirect transmission increased in time, depending on the cumulative number of days pigs tested positive for the presence of S. suis. The estimate β'ind was 0.001 (95% CI: 0.0006-0.0017 new infections per pig per day for each day that an infected pig was tested positive for S. suis. We conclude that prevention of direct contact reduces the rate at which susceptible pigs become colonized. Simulation studies using these parameters showed, however, that such intervention measure would not limit S. suis serotype 9 spread in a commercial pig farm to a relevant extent, implying that spatial separation of groups op pigs within a compartment would not be effective on a farm.

  17. La variable condición de actividad económica según el Censo Nacional 2000. Resultados y evaluación

    Directory of Open Access Journals (Sweden)

    Jorge A. Barquero B.

    2004-01-01

    Full Text Available El propósito de este artículo es presentar los resultados de la evaluación de la Condición de Actividad Económica obtenidos en el Censo 2000 de Costa Rica, mediante el análisis comparativo con la Encuesta de Hogares de Propósitos Múltiples de ese mismo año. A pesar de que en el Censo 2000 se tomaron medidas en el diseño del cuestionario y la organización del trabajo de campo, para tratar de minimizar errores durante el empadronamiento; las condiciones en que se llevó a cabo la capacitación y el levantamiento censal dificultaron la adecuada aplicación del cuestionario, sobre todo en las preguntas de empleo que requieren del dominio de mayor número de conceptos, definiciones e instrucciones más complejas. Esto dio lugar a que se presentaran problemas al indagar acerca del trabajo o búsqueda del mismo, especialmente en ciertos grupos de población. Las mujeres, las personas jóvenes y las residentes en zonas rurales fueron las más afectadas en la captación de su condición de actividad, mientras que para los hombres, sobre todo de zonas urbanas, se lograron mejores resultados. Pese a las limitaciones indicadas en este trabajo, los resultados censales conservan su validez pues generan información general que permite establecer puntos de referencia y cambios estructurales en el tiempo. Además, permiten obtener datos a nivel de provincia, cantón, distrito e incluso comunidades menores, lo cual no es posible mediante la Encuesta de Hogares. Resulta de particular interés presentar estos resultados, en especial a los usuarios especializados en materia de empleo, con el fin de advertir acerca de las principales bondades y limitaciones que posee esta información censal para distintos usos o aplicaciones. Se agradece la participación de todo el personal profesional y técnico del Instituto Nacional de Estadística y Censos (INEC, que colaboró en la producción de los cuadros y análisis que aparecen en este documento. No se

  18. Parasite population dynamics in pigs infected with Trichuris suis and Oesophagostomum dentatum

    DEFF Research Database (Denmark)

    Petersen, Heidi Huus; Andreasen, Annette; Kringel, Helene

    2014-01-01

    The aim of the present study was to investigate the population dynamics and potential interactions between Trichuris suis and Oesophagostomum dentatum in experimentally co-infected pigs, by quantification of parasite parameters such as egg excretion, worm recovery and worm location. Forty......-eight helminth naïve pigs were allocated into four groups. Group O was inoculated with 20 O. dentatum L3/kg/day and group T with 10 T. suis eggs/kg/day. Group OT was inoculated with both 20 O. dentatum L3/kg/day and 10 T. suis eggs/kg/day, while Group C was kept as an uninfected control group. All inoculations...

  19. O Censo de 2010 e as Primeiras Leituras Sobre a Mobilidade Espacial da População na Região Metropolitana de Campinas

    Directory of Open Access Journals (Sweden)

    Henrique Frey

    2013-09-01

    a produção do espaço urbano, ajudam-nos a entender o processo de localização espacial da população. O aumento da mobilidade ao longo das últimas décadas foi analisado em perspectiva comparada a partir dos quesitos sobre deslocamento pendular nos Censos Demográficos de 2000 e 2010. Deve-se ressaltar, ainda, que a manutenção da importância de Campinas para os deslocamentos diários e a alteração do peso destes fluxos para determinados municípios, podem estar associados a uma reorganização interna dos investimentos, da população e dos empregos.

  20. Characterization of Streptococcus suis through serotyping, SE-AFLP and virulence profile

    Directory of Open Access Journals (Sweden)

    Franco F. Calderaro

    Full Text Available Abstract: Streptococcus suis is one of most important pathogens in the swine industry worldwide. Despite its importance, studies of S. suis characterization in South America are still rare. This study evaluates S. suis isolates from distinct Brazilian states, from 1999 to 2004, and its molecular and serological characterization. A total of 174 isolates were studied. S. suis identification was confirmed by PCR and isolates were further serotyped and genotyped by SE-AFLP and amplification of virulence markers. Serotype 1, 2, 3, 4, 7, 18, 22 and 32 were identified among the studied isolates, and only 4% were characterized as non-typeable. The mrp+/epf+/sly+ genotype was the most frequent. The SE-AFLP analysis resulted in 29 patterns distributed in three main clusters with over 65% of genetic similarity. Isolates presented a slight tendency to cluster according to serotype and origin; however, no further correlation with virulence genotypes was observed.

  1. Virulence-associated gene profiling of Streptococcus suis isolates by PCR

    NARCIS (Netherlands)

    Silva, L.M.G.; Baums, C.G.; Rehm, T.; Wisselink, H.J.; Goethe, R.; Valentin-Weigand, P.

    2006-01-01

    Definition of virulent Streptococcus suis strains is controversial. One successful approach for identification of virulent European strains is differentiation of capsular serotypes (or the corresponding cps types) and subsequent detection of virulence-associated factors, namely the extracellular

  2. Antimicrobial activity of nisin against the swine pathogen Streptococcus suis and its synergistic interaction with antibiotics.

    Science.gov (United States)

    Lebel, Geneviève; Piché, Fanny; Frenette, Michel; Gottschalk, Marcelo; Grenier, Daniel

    2013-12-01

    Streptococcus suis serotype 2 is known to cause severe infections in pigs, including meningitis, endocarditis and pneumonia. Furthermore, this bacterium is considered an emerging zoonotic agent. Recently, increased antibiotic resistance in S. suis has been reported worldwide. The objective of this study was to evaluate the potential of nisin, a bacteriocin of the lantibiotic class, as an antibacterial agent against the pathogen S. suis serotype 2. In addition, the synergistic activity of nisin in combination with conventional antibiotics was assessed. Using a plate assay, the nisin-producing strain Lactococcus lactis ATCC 11454 proved to be capable of inhibiting the growth of S. suis (n=18) belonging to either sequence type (ST)1, ST25, or ST28. In a microdilution broth assay, the minimum inhibitory concentration (MIC) of purified nisin ranged between 1.25 and 5 μg/mL while the minimum bactericidal concentration (MBC) was between 5 and 10 μg/mL toward S. suis. The use of a capsule-deficient mutant of S. suis indicated that the presence of this polysaccharidic structure has no marked impact on susceptibility to nisin. Following treatment of S. suis with nisin, transmission electron microscopy observations revealed lysis of bacteria resulting from breakdown of the cell membrane. A time-killing curve showed a rapid bactericidal activity of nisin. Lastly, synergistic effects of nisin were observed in combination with several antibiotics, including penicillin, amoxicillin, tetracycline, streptomycin and ceftiofur. This study brought clear evidence supporting the potential of nisin for the prevention and treatment of S. suis infections in pigs. Copyright © 2013 Elsevier Inc. All rights reserved.

  3. Associations of Streptococcus suis serotype 2 ribotype profiles with clinical disease and antimicrobial resistance

    DEFF Research Database (Denmark)

    Rasmussen, S. R.; Aarestrup, Frank Møller; Jensen, N. E.

    1999-01-01

    A total of 122 Streptococcus suis serotype 2 strains were characterized thoroughly by comparing clinical and pathological observations, ribotype profiles, and antimicrobial resistance. Twenty-one different ribotype profiles were found and compared by cluster analysis, resulting in the identificat......A total of 122 Streptococcus suis serotype 2 strains were characterized thoroughly by comparing clinical and pathological observations, ribotype profiles, and antimicrobial resistance. Twenty-one different ribotype profiles were found and compared by cluster analysis, resulting...

  4. Interaction of Fibrinogen and Muramidase-released Protein Promotes the Development of Streptococcus suis Meningitis

    Directory of Open Access Journals (Sweden)

    Junping eWang

    2015-09-01

    Full Text Available Muramidase-released protein (MRP is as an important virulence marker of Streptococcus suis (S. suis serotype 2. Our previous works have shown that MRP can bind human fibrinogen (hFg; however, the function of this interaction in S.suis meningitis is not known. In this study, we found that the deletion of mrp significantly impairs the hFg-mediated adherence and traversal ability of S. suis across human cerebral microvascular endothelial cells (hCMEC/D3. Measurement of the permeability to Lucifer yellow in vitro and Evans blue extravasation in vivo show that the MRP-hFg interaction significantly increases the permeability of the blood-brain barrier (BBB. In the mouse meningitis model, wild type S. suis caused higher bacterial loads in the brain and more severe histopathological signs of meningitis than the mrp mutant at day 3 post-infection. Western blot analysis and immunofluorescence observations reveal that the MRP-hFg interaction can destroy the cell adherens junction protein p120-catenin of hCMEC/D3. These results indicate that the MRP-hFg interaction is important in the development of S. suis meningitis.

  5. Monographs in Tang Official Historiography: Perspectives from the Technical Treatises of the History of Sui (Sui shu)

    OpenAIRE

    Morgan, Daniel Patrick; Chaussende, Damien

    2018-01-01

    Finished in 656 as part of a larger project, the ten ‘treatises’ (zhi) of the current History of Sui (Sui shu) provide us with histories of such fields of technical knowledge as astronomy, astrology, omenology, economics, law, geography, metrology, and library science covering the five divided and short-lived dynasties, 502–618 CE, that were the predecessors of the Tang. This edited volume brings together young experts in these fields to reflect upon the way that their history has been writte...

  6. Streptococcal toxic shock syndrome caused by Streptococcus suis serotype 2.

    Directory of Open Access Journals (Sweden)

    Jiaqi Tang

    2006-05-01

    Full Text Available BACKGROUND: Streptococcus suis serotype 2 (S. suis 2, SS2 is a major zoonotic pathogen that causes only sporadic cases of meningitis and sepsis in humans. Most if not all cases of Streptococcal toxic shock syndrome (STSS that have been well-documented to date were associated with the non-SS2 group A streptococcus (GAS. However, a recent large-scale outbreak of SS2 in Sichuan Province, China, appeared to be caused by more invasive deep-tissue infection with STSS, characterized by acute high fever, vascular collapse, hypotension, shock, and multiple organ failure. METHODS AND FINDINGS: We investigated this outbreak of SS2 infections in both human and pigs, which took place from July to August, 2005, through clinical observation and laboratory experiments. Clinical and pathological characterization of the human patients revealed the hallmarks of typical STSS, which to date had only been associated with GAS infection. Retrospectively, we found that this outbreak was very similar to an earlier outbreak in Jiangsu Province, China, in 1998. We isolated and analyzed 37 bacterial strains from human specimens and eight from pig specimens of the recent outbreak, as well as three human isolates and two pig isolates from the 1998 outbreak we had kept in our laboratory. The bacterial isolates were examined using light microscopy observation, pig infection experiments, multiplex-PCR assay, as well as restriction fragment length polymorphisms (RFLP and multiple sequence alignment analyses. Multiple lines of evidence confirmed that highly virulent strains of SS2 were the causative agents of both outbreaks. CONCLUSIONS: We report, to our knowledge for the first time, two outbreaks of STSS caused by SS2, a non-GAS streptococcus. The 2005 outbreak was associated with 38 deaths out of 204 documented human cases; the 1998 outbreak with 14 deaths out of 25 reported human cases. Most of the fatal cases were characterized by STSS; some of them by meningitis or severe

  7. LA COMUNICAZIONE POLITICA SUI SOCIAL NETWORK: UN’ANALISI LINGUISTICA

    Directory of Open Access Journals (Sweden)

    Daniele Spoladore

    2014-07-01

    Full Text Available Questo lavoro si propone di analizzare le scelte linguistiche dei soggetti politici che utilizzano Facebook e Twitter per rivolgersi al sempre più frammentato panorama dei potenziali elettori. In particolare, analizza le tendenze linguistiche comuni che questi social network hanno fatto emergere nel rapporto tra politica, web e lettori. Cominciando dall’analisi della presenza di parlamentari e senatori su Facebook e Twitter, si cerca di riassumere le principali caratteristiche delle due piattaforme, per giungere ad una classificazione dei testi prodotti attraverso di esse; si analizza la scrittura di post e tweet, sottolineando la presenza di espedienti tipici del mezzo e valutando il loro effetto sui lettori, e si studiano le scelte sintattiche e morfosintattiche in relazione alla struttura e alle possibilità dei due social network. In ultima analisi si osservano le scelte lessicali dei soggetti politici, studiandone i campi semantici e la quantità di tecnicismi. Infine, si cerca di compiere una valutazione delle due differenti tipologie di trasmesso scritto rinvenute nei campioni, osservando come ognuna di esse abbia uno scopo ben preciso nell’economia della comunicazione politica sui social network. Political communication policy in social networks: a language analysis  Daniele Spoladore This paper aims to analyze the linguistic choices of politicians who use Facebook and Twitter to address the increasingly fragmented landscape of potential voters. In particular, it analyzes the common language trends that these social networks have revealed in the relationship between politics, the web and readers. Starting from the analysis of the presence of MPs and senators on Facebook and Twitter, we try to summarize the key features of the two platforms, in order to arrive at a classification of the texts produced using them.  We analyzed posts and tweets, emphasizing the  typical characteristics of these means and evaluating their effect on

  8. Genetic diversity of Streptococcus suis isolates as determined by comparative genome hybridization

    Directory of Open Access Journals (Sweden)

    Thi Hoa

    2011-07-01

    Full Text Available Abstract Background Streptococcus suis is a zoonotic pathogen that causes infections in young piglets. S. suis is a heterogeneous species. Thirty-three different capsular serotypes have been described, that differ in virulence between as well as within serotypes. Results In this study, the correlation between gene content, serotype, phenotype and virulence among 55 S. suis strains was studied using Comparative Genome Hybridization (CGH. Clustering of CGH data divided S. suis isolates into two clusters, A and B. Cluster A isolates could be discriminated from cluster B isolates based on the protein expression of extracellular factor (EF. Cluster A contained serotype 1 and 2 isolates that were correlated with virulence. Cluster B mainly contained serotype 7 and 9 isolates. Genetic similarity was observed between serotype 7 and serotype 2 isolates that do not express muramidase released protein (MRP and EF (MRP-EF-, suggesting these isolates originated from a common founder. Profiles of 25 putative virulence-associated genes of S. suis were determined among the 55 isolates. Presence of all 25 genes was shown for cluster A isolates, whereas cluster B isolates lacked one or more putative virulence genes. Divergence of S. suis isolates was further studied based on the presence of 39 regions of difference. Conservation of genes was evaluated by the definition of a core genome that contained 78% of all ORFs in P1/7. Conclusions In conclusion, we show that CGH is a valuable method to study distribution of genes or gene clusters among isolates in detail, yielding information on genetic similarity, and virulence traits of S. suis isolates.

  9. Capsular Sialyltransferase Specificity Mediates Different Phenotypes in Streptococcus suis and Group B Streptococcus

    Directory of Open Access Journals (Sweden)

    David Roy

    2018-04-01

    Full Text Available The capsular polysaccharide (CPS represents a key virulence factor for most encapsulated streptococci. Streptococcus suis and Group B Streptococcus (GBS are both well-encapsulated pathogens of clinical importance in veterinary and/or human medicine and responsible for invasive systemic diseases. S. suis and GBS are the only Gram-positive bacteria which express a sialylated CPS at their surface. An important difference between these two sialylated CPSs is the linkage between the side-chain terminal galactose and sialic acid, being α-2,6 for S. suis but α-2,3 for GBS. It is still unclear how sialic acid may affect CPS production and, consequently, the pathogenesis of the disease caused by these two bacterial pathogens. Here, we investigated the role of sialic acid and the putative effect of sialic acid linkage modification in CPS synthesis using inter-species allelic exchange mutagenesis. To this aim, a new molecular biogenetic approach to express CPS with modified sialic acid linkage was developed. We showed that sialic acid (and its α-2,6 linkage is crucial for S. suis CPS synthesis, whereas for GBS, CPS synthesis may occur in presence of an α-2,6 sialyltransferase or in absence of sialic acid moiety. To evaluate the effect of the CPS composition/structure on sialyltransferase activity, two distinct capsular serotypes within each bacterial species were compared (S. suis serotypes 2 and 14 and GBS serotypes III and V. It was demonstrated that the observed differences in sialyltransferase activity and specificity between S. suis and GBS were serotype unrestricted. This is the first time that a study investigates the interspecies exchange of capsular sialyltransferase genes in Gram-positive bacteria. The obtained mutants represent novel tools that could be used to further investigate the immunomodulatory properties of sialylated CPSs. Finally, in spite of common CPS structural characteristics and similarities in the cps loci, sialic acid exerts

  10. Reappraisal of the taxonomy of Streptococcus suis serotypes 20, 22 and 26: Streptococcus parasuis sp. nov.

    Science.gov (United States)

    Nomoto, R; Maruyama, F; Ishida, S; Tohya, M; Sekizaki, T; Osawa, Ro

    2015-02-01

    In order to clarify the taxonomic position of serotypes 20, 22 and 26 of Streptococcus suis, biochemical and molecular genetic studies were performed on isolates (SUT-7, SUT-286(T), SUT-319, SUT-328 and SUT-380) reacted with specific antisera of serotypes 20, 22 or 26 from the saliva of healthy pigs as well as reference strains of serotypes 20, 22 and 26. Comparative recN gene sequencing showed high genetic relatedness among our isolates, but marked differences from the type strain S. suis NCTC 10234(T), i.e. 74.8-75.7 % sequence similarity. The genomic relatedness between the isolates and other strains of species of the genus Streptococcus, including S. suis, was calculated using the average nucleotide identity values of whole genome sequences, which indicated that serotypes 20, 22 and 26 should be removed taxonomically from S. suis and treated as a novel genomic species. Comparative sequence analysis revealed 99.0-100 % sequence similarities for the 16S rRNA genes between the reference strains of serotypes 20, 22 and 26, and our isolates. Isolate STU-286(T) had relatively high 16S rRNA gene sequence similarity with S. suis NCTC 10234(T) (98.8 %). SUT-286(T) could be distinguished from S. suis and other closely related species of the genus Streptococcus using biochemical tests. Due to its phylogenetic and phenotypic similarities to S. suis we propose naming the novel species Streptococcus parasuis sp. nov., with SUT-286(T) ( = JCM 30273(T) = DSM 29126(T)) as the type strain. © 2015 IUMS.

  11. Swine infection by Streptococcus suis: a retrospective study Infecção em suínos por Streptococcus suis: estudo retrospectivo

    Directory of Open Access Journals (Sweden)

    A.E. Del'Arco

    2008-08-01

    Full Text Available The epidemic aspects of swine infections caused by Streptococcus suis were studied, focusing mainly on the occurrence of several serotypes. A total of 323 samples of S. suis were isolated from clinically ill animals, serotyped according to the co-agglutination procedure, and analyzed. The serotyping revealed that S. suis was present in several Brazilian states. The largest number was isolated from the states of Minas Gerais (62.5%, São Paulo (10.8%, and Paraná (9.3%. Serotype 2 was the most frequent (61.0%, followed by the serotypes 1, 3, 4, 7, and 8. The largest number of isolations was obtained from the brain (60.1%, followed by the lungs (10.4%. About 9.4% of the cases were due to septicemia.Estudaram-se os aspectos epidêmicos das infecções de suínos causadas por Streptococcus suis, enfocando, principalmente, a ocorrência de diferentes sorotipos. Foram analisadas 323 amostras isoladas de animais clinicamente doentes, as quais foram sorotipadas de acordo com o procedimento de co-aglutinação. Foi verificado que S. suis está presente em vários estados brasileiros e o maior número de isolados originou-se dos estados de Minas Gerais (62,5%, São Paulo (10,8% e Paraná (9,3%. O sorotipo 2 foi o mais freqüente (61.0%, seguido pelos sorotipos 1, 3, 4, 7 e 8. Os isolamentos foram obtidos principalmente de cérebro (60,1% e pulmões (10,4%. Os casos de septicemia representaram 9,4%.

  12. Extended semen for artificial insemination in swine as a potential transmission mechanism for infectious Chlamydia suis.

    Science.gov (United States)

    Hamonic, G; Pasternak, J A; Käser, T; Meurens, F; Wilson, H L

    2016-09-01

    Although typically unnoticed, Chlamydia infections in swine have been shown to be both widespread and may impact production characteristics and reproductive performance in swine. Serum titers suggest Chlamydia infection within boar studs is common, and infected boars are known to shed chlamydia in their ejaculates. Although the transmission of viruses in chilled extended semen (ES) is well established, the inclusion of antibiotics in commercially available extender is generally believed to limit or preclude the transmission of infectious bacteria. The objective of this study was to evaluate the potential of ES used in artificial insemination to support transmission of the obligate intracellular bacteria Chlamydia suis (C suis) under standard industry conditions. First, the effect of C suis on sperm quality during storage was assessed by flow cytometry. Only concentrations above 5 × 10(5) viable C suis/mL caused significant spermicidal effects which only became evident after 7 days of storage at 17 °C. No significant effect on acrosome reaction was observed using any chlamydial concentration. Next, an in vitro infection model of swine testicular fibroblast cells was established and used to evaluate the effect of chilled storage on C suis viability under variable conditions. Storage in Androhep ES reduced viability by 34.4% at a multiplicity of infection of 1.25, an effect which increased to 53.3% when the multiplicity of infection decreased to 0.1. Interestingly, storage in semen extender alone (SE) or ES with additional antibiotics had no effect on bacterial viability. To rule out a secondary effect on extender resulting from metabolically active sperm, C suis was stored in fresh and expended SE and again no significant effect on bacterial viability was observed. Fluorescent microscopy of C suis in ES shows an association between bacteria and the remaining gel fraction after storage suggesting that the apparent reduction of bacterial viability in the presence

  13. Transcriptomic Analysis Reveals Selective Metabolic Adaptation of Streptococcus suis to Porcine Blood and Cerebrospinal Fluid

    Directory of Open Access Journals (Sweden)

    Anna Koczula

    2017-02-01

    Full Text Available Streptococcus suis is a zoonotic pathogen that can cause severe pathologies such as septicemia and meningitis in its natural porcine host as well as in humans. Establishment of disease requires not only virulence of the infecting strain but also an appropriate metabolic activity of the pathogen in its host environment. However, it is yet largely unknown how the streptococcal metabolism adapts to the different host niches encountered during infection. Our previous isotopologue profiling studies on S. suis grown in porcine blood and cerebrospinal fluid (CSF revealed conserved activities of central carbon metabolism in both body fluids. On the other hand, they suggested differences in the de novo amino acid biosynthesis. This prompted us to further dissect S. suis adaptation to porcine blood and CSF by RNA deep sequencing (RNA-seq. In blood, the majority of differentially expressed genes were associated with transport of alternative carbohydrate sources and the carbohydrate metabolism (pentose phosphate pathway, glycogen metabolism. In CSF, predominantly genes involved in the biosynthesis of branched-chain and aromatic amino acids were differentially expressed. Especially, isoleucine biosynthesis seems to be of major importance for S. suis in CSF because several related biosynthetic genes were more highly expressed. In conclusion, our data revealed niche-specific metabolic gene activity which emphasizes a selective adaptation of S. suis to host environments.

  14. Limited Interactions between Streptococcus Suis and Haemophilus Parasuis in In Vitro Co-Infection Studies

    Directory of Open Access Journals (Sweden)

    Annabelle Mathieu-Denoncourt

    2018-01-01

    Full Text Available Streptococcus suis and Haemophilus parasuis are normal inhabitants of the porcine upper respiratory tract but are also among the most frequent causes of disease in weaned piglets worldwide, causing inflammatory diseases such as septicemia, meningitis and pneumonia. Using an in vitro model of infection with tracheal epithelial cells or primary alveolar macrophages (PAMs, it was possible to determine the interaction between S. suis serotype 2 and H. parasuis strains with different level of virulence. Within H. parasuis strains, the low-virulence F9 strain showed higher adhesion levels to respiratory epithelial cells and greater association levels to PAMs than the high-virulence Nagasaki strain. Accordingly, the low-virulence F9 strain induced, in general, higher levels of pro-inflammatory cytokines than the virulent Nagasaki strain from both cell types. In general, S. suis adhesion levels to respiratory epithelial cells were similar to H. parasuis Nagasaki strain. Yet, S. suis strains induced a significantly lower level of pro-inflammatory cytokine expression from epithelial cells and PAMs than those observed with both H. parasuis strains. Finally, this study has shown that, overall and under the conditions used in the present study, S. suis and H. parasuis have limited in vitro interactions between them and use probably different host receptors, regardless to their level of virulence.

  15. Limited Interactions between Streptococcus Suis and Haemophilus Parasuis in In Vitro Co-Infection Studies

    Science.gov (United States)

    Mathieu-Denoncourt, Annabelle; Letendre, Corinne; Auger, Jean-Philippe; Segura, Mariela; Aragon, Virginia; Lacouture, Sonia; Gottschalk, Marcelo

    2018-01-01

    Streptococcus suis and Haemophilus parasuis are normal inhabitants of the porcine upper respiratory tract but are also among the most frequent causes of disease in weaned piglets worldwide, causing inflammatory diseases such as septicemia, meningitis and pneumonia. Using an in vitro model of infection with tracheal epithelial cells or primary alveolar macrophages (PAMs), it was possible to determine the interaction between S. suis serotype 2 and H. parasuis strains with different level of virulence. Within H. parasuis strains, the low-virulence F9 strain showed higher adhesion levels to respiratory epithelial cells and greater association levels to PAMs than the high-virulence Nagasaki strain. Accordingly, the low-virulence F9 strain induced, in general, higher levels of pro-inflammatory cytokines than the virulent Nagasaki strain from both cell types. In general, S. suis adhesion levels to respiratory epithelial cells were similar to H. parasuis Nagasaki strain. Yet, S. suis strains induced a significantly lower level of pro-inflammatory cytokine expression from epithelial cells and PAMs than those observed with both H. parasuis strains. Finally, this study has shown that, overall and under the conditions used in the present study, S. suis and H. parasuis have limited in vitro interactions between them and use probably different host receptors, regardless to their level of virulence. PMID:29316613

  16. Stimulating the development of national Streptococcus suis guidelines in Viet Nam through a strategic research partnership.

    Science.gov (United States)

    Horby, Peter; Wertheim, Heiman; Ha, Nguyen Hong; Trung, Nguyen Vu; Trinh, Dao Tuyet; Taylor, Walter; Ha, Nguyen Minh; Lien, Trinh Thi Minh; Farrar, Jeremy; Van Kinh, Nguyen

    2010-06-01

    Streptococcus suis is a common cause of adult bacterial meningitis in Viet Nam, and possibly other parts of Asia, yet this disabling infection has been largely neglected. Prevention, diagnosis and treatment are relatively straightforward and affordable but, in early 2007, no national diagnostic, case management or prevention guidelines existed in Viet Nam. Enhanced detection of S. suis infections was established in 2007 as part of a collaborative research programme between the National Hospital for Tropical Diseases, a key national hospital with very close links to the Ministry of Health, and a research group affiliated with Oxford University based in Viet Nam. The results were reported directly to policy-makers at the Ministry of Health. Viet Nam is a low-income country with a health-care system that has seen considerable improvements and increased autonomy. However, parts of the system remain fairly centralized the Ministry of Health. Following the improved detection and reporting of S. suis cases, the Ministry of Health issued guidance to all hospitals in Viet Nam on the clinical and laboratory diagnosis, treatment and prevention of S. suis. A public health laboratory diagnostic service was established at the National Institute of Hygiene and Epidemiology and training courses were conducted for clinicians and microbiologists. Ministry of Health guidance on surveillance and control of communicable diseases was updated to include a section on S. suis. Research collaborations can efficiently inform and influence national responses if they are well positioned to reach policy-makers.

  17. Saccharomyces boulardii administration can inhibit the formation of gastric lymphoid follicles induced by Helicobacter suis infection.

    Science.gov (United States)

    Yang, Lin; Tian, Zi-Bin; Yu, Ya-Nan; Zhang, Cui-Ping; Li, Xiao-Yu; Mao, Tao; Jing, Xue; Zhao, Wen-Jun; Ding, Xue-Li; Yang, Ruo-Ming; Zhang, Shuai-Qing

    2017-01-01

    Helicobacter suis has a greater tendency to induce gastric mucosa-associated lymphoid tissue lymphoma compared with other Helicobacter species in humans and animals. Saccharomyces boulardii has been established as an adjunct to H. pylori eradication treatment, but the effect of S. boulardii administration alone on Helicobacter infection remains unclear. Here, we found that S. boulardii administration effectively decreased the bacterial load of H. suis and inhibited the formation of lymphoid follicles in the stomach post-infection. The levels of H. suis-specific immunoglobulin A (IgA) and secretory IgA in the gastric juice and small intestinal secretions and the production of mouse β-defensin-3 in the small intestinal secretions were significantly increased by S. boulardii administration at 12 weeks after H. suis infection. In addition, feeding with S. boulardii inhibited the expression of inflammatory cytokines and lymphoid follicle formation-related factors after H. suis infection. These results suggested that S. boulardii may be useful for the prevention and treatment of Helicobacter infection-related diseases in humans. © FEMS 2017. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

  18. Study on bioactive compounds of in vitro cultured Calculus Suis and natural Calculus Bovis.

    Science.gov (United States)

    Wan, Tien-Chun; Cheng, Fu-Yuan; Liu, Yu-Tse; Lin, Liang-Chuan; Sakata, Ryoichi

    2009-12-01

    The purpose of the study was to investigate bioactive compounds of in vitro cultured Calculus Suis and natural Calculus Bovis obtained as valuable by-products from animals used for meat production. The results showed that the components of natural Calculus Bovis were rich in bilirubin and biliverdin and had higher content of essential amino acids. The major amino acids of in vitro cultured Calculus Suis were identified as glycine, alanine, glutamic acid and aspartic acid, and those for natural Calculus Bovis were found to be glutamic acid, aspartic acid, proline, and arginine. The methionine and cysteine contents of precursors for glutathione in natural Calculus Bovis were significantly higher than those of in vitro cultured Calculus Suis. The mineral contents of zinc, iron and manganese of natural Calculus Bovis were significantly higher than those of in vitro cultured Calculus Suis. The major bile acids in both products were cholic acid and dehydrocholic acid, respectively. The chenodeoxycholic and ursodeoxycholic acid content of in vitro cultured Calculus Suis was significantly higher than that of natural Calculus Bovis.

  19. Las probabilidades de agrandamiento de la familia y la fecundidad por orden de nacimiento en Sonora, según los censos de 1980 y 1990

    Directory of Open Access Journals (Sweden)

    Patricia Olga Hernández Espinoza

    1998-01-01

    Full Text Available En este trabajo se analiza la variación de la fecundidad de las mujeres cuyo periodo reproductivo finalizaba al momento de llevarse a cabo los censos de 1980 y 1990, esto es, las mujeres de la generación 1930-1934, que al 4 de junio de 1980 tenían entre 45 y 49 años de edad, y las mujeres de la generación 1940-1944, que al 12 de marzo de 1990 estaban en el grupo de edad mencionado. Este análisis se realizó con información censal a nivel nacional, del estado de Sonora, y de cada uno de sus municipios, mediante el cálculo de las probabilidades de agrandamiento de la familia, para observar los cambios en los patrones de formación de la descendencia. Los resultados muestran que en los tres niveles de desagregación hay una tendencia a disminuir el número de hijos que conformaban las familias mexicanas y en particular las sonorenses.

  20. Impact of Sub-Inhibitory Concentrations of Amoxicillin on Streptococcus suis Capsule Gene Expression and Inflammatory Potential

    Directory of Open Access Journals (Sweden)

    Bruno Haas

    2016-04-01

    Full Text Available Streptococcus suis is an important swine pathogen and emerging zoonotic agent worldwide causing meningitis, endocarditis, arthritis and septicemia. Among the 29 serotypes identified to date, serotype 2 is mostly isolated from diseased pigs. Although several virulence mechanisms have been characterized in S. suis, the pathogenesis of S. suis infections remains only partially understood. This study focuses on the response of S. suis P1/7 to sub-inhibitory concentrations of amoxicillin. First, capsule expression was monitored by qRT-PCR when S. suis was cultivated in the presence of amoxicillin. Then, the pro-inflammatory potential of S. suis P1/7 culture supernatants or whole cells conditioned with amoxicillin was evaluated by monitoring the activation of the NF-κB pathway in monocytes and quantifying pro-inflammatory cytokines secreted by macrophages. It was found that amoxicillin decreased capsule expression in S. suis. Moreover, conditioning the bacterium with sub-inhibitory concentrations of amoxicillin caused an increased activation of the NF-κB pathway in monocytes following exposure to bacterial culture supernatants and to a lesser extent to whole bacterial cells. This was associated with an increased secretion of pro-inflammatory cytokines (CXCL8, IL-6, IL-1β by macrophages. This study identified a new mechanism by which S. suis may increase its inflammatory potential in the presence of sub-inhibitory concentrations of amoxicillin, a cell wall-active antibiotic, thus challenging its use for preventive treatments or as growth factor.

  1. The whipworm (Trichuris suis) secretes prostaglandin E2 to suppress proinflammatory properties in human dendritic cells

    DEFF Research Database (Denmark)

    Laan, Lisa C; Williams, Andrew R; Stavenhagen, Kathrin

    2017-01-01

    Clinical trials have shown that administration of the nematode Trichuris suis can be beneficial in treating various immune disorders. To provide insight into the mechanisms by which this worm suppresses inflammatory responses, an active component was purified from T. suis soluble products (TsSPs)...

  2. Development and validation of a real-time PCR for Chlamydia suis diagnosis in swine and humans.

    Directory of Open Access Journals (Sweden)

    Kristien De Puysseleyr

    Full Text Available Pigs are the natural host for Chlamydia suis, a pathogen which is phylogenetically highly related to the human pathogen C. trachomatis. Chlamydia suis infections are generally treated with tetracyclines. In 1998, tetracyline resistant C. suis strains emerged on U.S. pig farms and they are currently present in the Belgian, Cypriote, German, Israeli, Italian and Swiss pig industry. Infections with tetracycline resistant C. suis strains are mainly associated with severe reproductive failure leading to marked economical loss. We developed a sensitive and specific TaqMan probe-based C. suis real-time PCR for examining clinical samples of both pigs and humans. The analytical sensitivity of the real-time PCR is 10 rDNA copies/reaction without cross-amplifying DNA of other Chlamydia species. The PCR was successfully validated using conjunctival, pharyngeal and stool samples of slaughterhouse employees, as well as porcine samples from two farms with evidence of reproductive failure and one farm without clinical disease. Chlamydia suis was only detected in diseased pigs and in the eyes of humans. Positive humans had no clinical complaints. PCR results were confirmed by culture in McCoy cells. In addition, Chlamydia suis isolates were also examined by the tet(C PCR, designed for demonstrating the tetracycline resistance gene tet(C. The tet(C gene was only present in porcine C. suis isolates.

  3. Differentiation between serological responses to Brucella suis and Yersinia enterocolitica serotype O : 9 after natural or experimental infection in pigs

    DEFF Research Database (Denmark)

    Jungersen, Gregers; Sørensen, Vibeke; Giese, Steen Bjørck

    2006-01-01

    with responses of B. suis biovar 2-inoculated pigs. FPSR were limited to 2-9 weeks post-YeO:9 inoculation, while B. suis-infected pigs were test-positive throughout the 21-week period of investigation. Although YeO:9-inoculated pigs exhibited FPSR in Brucella tests for a limited period of time, the serological...

  4. Detection of Streptococcus suis by in situ hybridization, indirect immunofluorescence, and peroxidase-antiperoxidase assays in formalin-fixed, paraffin-embedded tissue sections from pigs

    DEFF Research Database (Denmark)

    Boye, Mette; Feenstra, Anne Avlund; Tegtmeier, Conny

    2000-01-01

    and the immunohistochemical methods were used for detection of S. suis in formalin-fixed, paraffin-embedded tissue sections of brain, endocardium, and lung from pigs infected with S. suis. The methods developed were able to detect single cells of S. suis in situ in the respective samples, whereas no signal was observed from...

  5. Development, Hatching, and Intestinal Establishment of Trichuris suis, - in vivo and in vitro Studies

    DEFF Research Database (Denmark)

    Vejzagic, Nermina

    Trichuris suis, the pig whipworm is a nematode parasite located in the large intestine of pigs. Embryonated eggs of T. suis (T. suis ova = TSO) constitute the active pharmaceutical ingredient (API) in a new medicinal product, which is currently tested in human clinical trials as a potential treat...

  6. Antimicrobial susceptibility of Streptococcus suis isolated from clinically healthy swine in Brazil.

    Science.gov (United States)

    Soares, Taíssa Cook Siqueira; Paes, Antonio Carlos; Megid, Jane; Ribolla, Paulo Eduardo Martins; Paduan, Karina dos Santos; Gottschalk, Marcelo

    2014-04-01

    Streptococcus suis is an important pathogen in the swine industry. This study is the first to report on the antimicrobial susceptibility of S. suis isolated from clinically healthy pigs in Brazil; the fourth major pork producer in the world. The antimicrobial susceptibility of 260 strains was determined by disc diffusion method. Strains were commonly susceptible to ceftiofur, cephalexin, chloramphenicol, and florfenicol, with more than 80% of the strains being susceptible to these antimicrobials. A high frequency of resistance to some of the antimicrobial agents was demonstrated, with resistance being most common to sulfa-trimethoprim (100%), tetracycline (97.69%), clindamycin (84.61%), norfloxacin (76.92%), and ciprofloxacin (61.15%). A high percentage of multidrug resistant strains (99.61%) were also found. The results of this study indicate that ceftiofur, cephalexin, and florfenicol are the antimicrobials of choice for empirical control of the infections caused by S. suis.

  7. Bacteria-induced egg hatching differs for Trichuris muris and Trichuris suis

    DEFF Research Database (Denmark)

    Vejzagic, Nermina; Adelfio, Roberto; Keiser, Jennifer

    2015-01-01

    BACKGROUND: Eggs of the porcine whipworm Trichuris suis are currently explored in human clinical trials as a treatment of immune-mediated diseases. In this context, only the infective, embryonated eggs, constitute the Active Pharmaceutical Ingredient (API). The rodent whipworm, Trichuris muris...... is commonly used as a laboratory model to study Trichuris biology. The embryonated eggs (containing a fully developed larva) are biologically active and will invade the large intestinal mucosa of the host. This study aims to assess the in vitro hatching of T. muris and T. suis eggs in various bacterial...... cultures as a measure for their biological activity. METHODS: Eggs of T. muris and T. suis were incubated with Escherichia coli strain (BL-21) at three concentrations in a slightly modified in vitro egg hatching assay previously developed for T. muris. Additionally, E. coli strains (M15, SG13009, PMC103...

  8. Seroepidemiologic survey for Chlamydia suis in wild boar (Sus scrofa) populations in Italy.

    Science.gov (United States)

    Di Francesco, Antonietta; Donati, Manuela; Morandi, Federico; Renzi, Maria; Masia, Marco Antonio; Ostanello, Fabio; Salvatore, Daniela; Cevenini, Roberto; Baldelli, Raffaella

    2011-07-01

    We used serology to estimate the prevalence of exposure to chlamydiae in Italian populations of wild boars (Sus scrofa). Sera from 173 hunter-killed wild boars harvested during the 2006-2009 hunting seasons in three Italian regions were tested for antibodies to Chlamydia suis, Chlamydophila pecorum, Chlamydophila abortus, and Chlamydophila psittaci by the microimmunofluorescence test. Antibody titers to chlamydiae ≥ 1:32 were detected in 110 of the 173 samples tested (63.6%). Specific reactivity could be assessed only in 44 sera with antibody titers to C. suis that were two- to threefold higher than antibody titers against the other chlamydial species; the other 66 sera had similar reactivity against all the chlamydia species tested. Antibody to C. suis was detected in sera from wild boar populations with rare or no known contact with domestic pigs. These results suggest that the wild boar could be a chlamydia reservoir and may acquire chlamydiae independent of contacts with the domestic pig.

  9. Use of Tetravalent Galabiose for Inhibition of Streptococcus Suis Serotype 2 Infection in a Mouse Model

    Directory of Open Access Journals (Sweden)

    Karen A. Krogfelt

    2013-04-01

    Full Text Available Streptococcus suis is an important swine pathogen associated with a variety of infections such as meningitis, arthritis and septicemia. The bacterium is zoonotic and has been found to cause meningitis especially in humans occupationally exposed to infected pigs. Since adhesion is a prerequisite for colonization and subsequent infection, anti-adhesion treatment seems a natural alternative to traditional treatment with antibiotics. In order to optimize the inhibitory potency a multivalency approach was taken in the inhibitor design. A synthetic tetravalent galabiose compound was chosen which had previously shown promising anti-adhesion effects with S. suis in vitro. The aim of this study was to evaluate the in vivo effects of the compound using an infection peritonitis mouse model. As such S. suis serotype 2 infection and treatment were tested in vivo and the effects were compared to the effect of treatment with penicillin.

  10. Symptoms after ingestion of pig whipworm Trichuris suis eggs in a randomized placebo-controlled double-blind clinical trial

    DEFF Research Database (Denmark)

    Bager, Peter; Kapel, Christian Moliin Outzen; Roepstorff, Allan Knud

    2011-01-01

    by a fluoroenzymeimmunoassay (Phadia ApS). During 163 days complete follow-up, subjects ingesting T. suis eggs (N = 49) had a three to 19-fold higher rate of events (median duration, 2 days) with gastrointestinal reactions (moderate to severe flatulence, diarrhea, and upper abdominal pain) compared with placebo subjects (N......Symptoms after human infection with the helminth Trichuris suis have not previously been described. Exposure to helminths has been suggested as immune therapy against allergy and autoimmune diseases. We randomized adults with allergic rhinitis to ingest a dose of 2500 T. suis eggs or placebo every......-hoc analyses of gastrointestinal reactions. Adverse events and severity (mild, moderate, severe) were recorded daily by subjects, classified by organ using MedDRA 10.0, and event rates compared between subjects on T. suis treatment vs. subjects on placebo. T. suis-specific serum IgG antibodies were measured...

  11. Pathway of oxfendazole from the host into the worm: Trichuris suis in pigs

    Directory of Open Access Journals (Sweden)

    Tina V.A. Hansen

    2017-12-01

    Full Text Available It is well known that the efficacy of a single oral dose of benzimidazoles against Trichuris spp. infections in humans and animals is poor, but is currently still used in control programmes against human trichuriasis. However, the route of the benzimidazoles from the treated host to Trichuris remains unknown. As parts of adult Trichuris are situated intracellularly in the caecum, they might be exposed to anthelmintic drugs in the intestinal content as well as the mucosa. In this study, the pathway of oxfendazole and its metabolites was explored using a T. suis-pig infection model, by simultaneously measuring drug concentrations within the worms and the caecal mucosa, caecal tissue, caecal content and plasma of pigs over time after a single oral dose of 5 mg/kg oxfendazole. Additionally, for comparison to the in vivo study, drug uptake and metabolism of oxfendazole by T. suis was examined after in vitro incubation. Oxfendazole and metabolites were quantified by High Performance Liquid Chromatography.Multivariate linear regression analysis showed a strong and highly significant association between OFZ concentrations within T. suis and in plasma, along with a weaker association between OFZ concentrations in caecal tissue/mucosa and T. suis, suggesting that oxfendazole reaches T. suis after absorption from the gastrointestinal tract and enters the worms by the blood-enterocyte pathway. The fenbendazole sulfone level in T. suis was highly affected by the concentrations in plasma. In addition, correlations between drug concentrations in the host compartments, were generally highest for this metabolite. In comparison to oxfendazole, the correlation between plasma and content was particularly high for this metabolite, suggesting a high level of drug movement between these compartments and the possible involvement of the enterohepatic circulation. Keywords: Trichuris, Benzimidazole, Drug efficacy, Drug pathway

  12. Molecular identification of Trichuris vulpis and Trichuris suis isolated from different hosts.

    Science.gov (United States)

    Cutillas, Cristina; de Rojas, Manuel; Ariza, Concepción; Ubeda, José Manuel; Guevara, Diego

    2007-01-01

    Trichuris suis was isolated from the cecum of two different hosts (Sus scrofa domestica -- swine and Sus scrofa scrofa -- wild boar) and Trichuris vulpis from dogs in Sevilla, Spain. Genomic DNA was isolated and internal transcribed spacers (ITS)1-5.8S-ITS2 segment from the ribosomal DNA (rDNA) was amplified and sequenced using polymerase chain reaction techniques. The sequence of T. suis from both hosts was 1,396 bp in length while that of T. vulpis was 1,044 bp. ITS1 of both populations isolated of T. suis was 661 nucleotides in length, while the ITS2 was 534 nucleotides in length. Furthermore, the ITS1 of T. vulpis was 410 nucleotides in length, while the ITS2 was 433 nucleotides in length. One hundred fifty-four nucleotides were observed along the 5.8S gene of T. suis and T. vulpis. Intraindividual and intraspecific variations were detected in the rDNA of both species. The presence of microsatellites was observed in all the individuals assayed. Sequence analysis of the ITSs and the 5.8S gene has demonstrated no sequence differences between T. suis isolated from both hosts (S. scrofa domestica -- swine and S. scrofa scrofa -- wild boar). Nevertheless, clear differences were detected between the ITS1 and ITS2 of T. suis and T. vulpis. Furthermore, a comparative molecular analysis between both species and the previously published ITS1-5.8S-ITS2 sequence data of Trichuris ovis, Trichuris leporis, Trichuris muris, Trichuris arvicolae, and Trichuris skrjabini was carried out. A common homology zone was detected in the ITS1 sequence of all species of trichurids.

  13. Streptococcus suis meningoencephalitis with seizure from raw pork ingestion: a case report.

    Science.gov (United States)

    Wongjittraporn, Suwarat; Teerasukjinda, Ornusa; Yee, Melvin; Chung, Heath H

    2014-09-01

    Streptococcus suis meningoencephalitis is a rare but increasingly important condition. Good history taking will give clues to the diagnosis. This is the fourth case report in the United States. A 52-year-old Filipino man who recently returned from a trip to the Philippines was admitted with classic symptoms of bacterial meningitis. His cerebrospinal fluid culture grew Streptococcus suis. His clinical course was complicated by seizures, hearing loss, and permanent tinnitus. Clinicians should be aware of this emerging disease especially in patients with recent travel history to endemic areas. Early recognition and appropriate management could potentially prevent complications.

  14. On Furuto-sui in Chikuho coal field. Chikuho tanden no furutosui ni tsuite

    Energy Technology Data Exchange (ETDEWEB)

    Yamashita, Akio [Kyushu Univ., Faculty of Engineering, Fukuoka (Japan)

    1989-11-30

    This paper described the Furuto-sui(goaf water) in Chikuho Coal Field where coal mining was made for 100 years, remaining mined traces in wide range. This is called Furuto and water collected in or passed through Furuto is called Furuto-sui. The Furuto was made by coal layer mining and the distribution is coincident to the geological structure of coal layer. This Furuto structure is shallower to westward direction, deeper to eastward direction and identical to the north-southward direction. The Furuto can be said as the artificially built reservior for ground water. The coal mining was perfectly stopped in 1976 and the Furuto-sui filled in mined cavities. At low ground area, springs or humid areas appeared. Water quality is diverse but the typical ones are the type of Ca-SO{sub 4} of 3-5 pH, type of Na-HCO{sub 3}*SO{sub 4} of 8-9pH, and type of Na-SO{sub 4} of 7 pH. A part of Furuto-sui is utilized as the water for home use but must be utilized much more as water reservior. 12 refs., 9 figs.

  15. Control of Competence for DNA Transformation in Streptococcus suis by Genetically Transferable Pherotypes

    NARCIS (Netherlands)

    Zaccaria, E.; Baarlen, van P.; Greeff, de A.; Morrison, D.A.; Smith, H.; Wells, J.M.

    2014-01-01

    Here we show that S. suis, a major bacterial pathogen of pigs and emerging pathogen in humans responds to a peptide pheromone by developing competence for DNA transformation. This species does not fall within any of the phylogenetic clusters of streptococci previously shown to regulate competence

  16. Expulsion of the swine whipworm, Trichuris suis 

    DEFF Research Database (Denmark)

    Kringel, Helene

    demonstrating the potential use of its eggs as immunomodulators, treating patients suffering from inflammatory bowel disease and possibly other autoimmune diseases. The immune response induced by T. suis in its host and particularly, the resulting expulsion of worms is the focus of this thesis. Whipworms...

  17. Trichuris suis ova therapy for allergic rhinitis

    DEFF Research Database (Denmark)

    Bager, Peter; Arnved, John; Rønborg, Steen

    2010-01-01

    Parasitic helminth infections can protect against allergic airway inflammation in experimental models and have been associated with a reduced risk of atopy and a reduced course of asthma in some observational studies. Although no clinical evidence exists to support the use of helminth therapy...... for allergic disease, the helminth Trichuris suis has demonstrated efficacy in treatment of inflammatory bowel disease....

  18. Prevalence and pathogenicity of Cryptosporidium suis in pre- and post-weaned pigs

    Czech Academy of Sciences Publication Activity Database

    Vítovec, J.; Hamadejová, K.; Landová, L.; Kváč, Martin; Květoňová, Dana; Sak, Bohumil

    2006-01-01

    Roč. 53, č. 5 (2006), s. 239-243 ISSN 0931-1793 R&D Projects: GA ČR GA524/05/0992 Institutional research plan: CEZ:AV0Z60220518 Keywords : Cryptosporidium suis * piglets * pathogenicity Subject RIV: EG - Zoology Impact factor: 1.356, year: 2006

  19. Immune and inflammatory responses in pigs infected with Trichuris suis and Oesophagostomum dentatum

    DEFF Research Database (Denmark)

    Andreasen, Annette; Petersen, Heidi Huus; Kringel, Helene

    2015-01-01

    effect on O. dentatum populations has been observed in co-infected pigs. Forty-eight helminth naïve pigs were allocated into 4 groups in a 2-factorial design. Two groups were trickle inoculated with either 10 T. suis eggs/kg/day (Group T) or 20 O. dentatum L3/kg/day (Group O). Group OT was infected...

  20. Streptococcus suis, an emerging drug-resistant animal and human pathogen

    Directory of Open Access Journals (Sweden)

    Claudio ePalmieri

    2011-11-01

    Full Text Available Streptococcus suis, a major porcine pathogen, has been receiving growing attention not only for its role in severe and increasingly reported infections in humans, but also for its involvement in drug resistance. Recent studies and the analysis of sequenced genomes have been providing important insights into the S. suis resistome, and have resulted in the identification of resistance determinants for tetracyclines, macrolides, aminoglycosides, chloramphenicol, antifolate drugs, streptothricin, and cadmium salts. Resistance gene-carrying genetic elements described so far include integrative and conjugative elements, transposons, genomic islands, phages, and chimeric elements. Some of these elements are similar to those reported in major streptococcal pathogens such as Streptococcus pyogenes, Streptococcus pneumoniae, and Streptococcus agalactiae and share the same chromosomal insertion sites. The available information strongly suggests that S. suis is an important antibiotic resistance reservoir that can contribute to the spread of resistance genes to the above-mentioned streptococci. S. suis is thus a paradigmatic example of possible intersections between animal and human resistomes.

  1. Immunogenicity and efficacy of a rough Brucella suis vaccine delivered orally or parenterally to feral swine

    Science.gov (United States)

    Brucella suis strain 353-1 is a stable vaccine strain that is clinically safe, does not cause positive serologic responses on conventional brucellosis surveillance tests, and induces humoral and cellular immunity in swine after vaccination. In this study, we evaluated tissue clearance and immunologi...

  2. Immuogenicity and safety of a natural rough mutant of Brucella suis as a vaccine for swine

    Science.gov (United States)

    The objective of the current study was to evaluate the safety, immunogenicity and clearance of the natural rough mutant of Brucella suis strain 353-1 (353-1) as a vaccine in domestic swine. In three studies encompassing 155 animals, pigs were inoculated with 353-1 by conjunctival (5 x 10**7 CFU), p...

  3. A genetic analysis of Trichuris trichiura and Trichuris suis from Ecuador.

    Science.gov (United States)

    Meekums, Hayley; Hawash, Mohamed B F; Sparks, Alexandra M; Oviedo, Yisela; Sandoval, Carlos; Chico, Martha E; Stothard, J Russell; Cooper, Philip J; Nejsum, Peter; Betson, Martha

    2015-03-19

    Since the nematodes Trichuris trichiura and T. suis are morphologically indistinguishable, genetic analysis is required to assess epidemiological cross-over between people and pigs. This study aimed to clarify the transmission biology of trichuriasis in Ecuador. Adult Trichuris worms were collected during a parasitological survey of 132 people and 46 pigs in Esmeraldas Province, Ecuador. Morphometric analysis of 49 pig worms and 64 human worms revealed significant variation. In discriminant analysis morphometric characteristics correctly classified male worms according to host species. In PCR-RFLP analysis of the ribosomal Internal Transcribed Spacer (ITS-2) and 18S DNA (59 pig worms and 82 human worms), nearly all Trichuris exhibited expected restriction patterns. However, two pig-derived worms showed a "heterozygous-type" ITS-2 pattern, with one also having a "heterozygous-type" 18S pattern. Phylogenetic analysis of the mitochondrial large ribosomal subunit partitioned worms by host species. Notably, some Ecuadorian T. suis clustered with porcine Trichuris from USA and Denmark and some with Chinese T. suis. This is the first study in Latin America to genetically analyse Trichuris parasites. Although T. trichiura does not appear to be zoonotic in Ecuador, there is evidence of genetic exchange between T. trichiura and T. suis warranting more detailed genetic sampling.

  4. Draft genome sequences of nine Streptococcus suis strains isolated in the United States

    Science.gov (United States)

    Streptococcus suis is a swine pathogen responsible for economic losses to the pig industry worldwide. Additionally, it is a zoonotic agent that can cause severe infections in those in close contact with infected pigs and/or who consume uncooked or undercooked pork products. Here, we report nine draf...

  5. Trichuris suis secrete products that reduce disease severity in a multiple sclerosis model

    DEFF Research Database (Denmark)

    Hansen, Christine Soholm; Hasseldam, Henrik; Bacher, Idahella Hyldgaard

    2017-01-01

    , including parasites. Parasites are known to employ different immunomodulatory and antiinflammatory strategies, which enable them to evade destruction by the immune system. We have investigated the immunomodulation by the swine whipworm, Trichuris suis, by measuring the impact of oral administration of T...

  6. Incidence and presence of virulence factors of Streptococcus suis infection in slaughtered pigs from Chiang Mai, Thailand.

    Science.gov (United States)

    Padungtod, Pawin; Tharavichitkul, Prasit; Junya, Supansa; Chaisowong, Warangkhana; Kadohira, Mutsuyo; Makino, Souichi; Sthitmatee, Nattawooti

    2010-11-01

    This study was designed to determine the incidence of Streptococcus suis infection in slaughtered pigs raised in industrial facility and backyard system in Chiang Mai City, Thailand. A total of 90 tonsils and submaxillary salivary gland/lymph node samples from slaughtered pigs raised in industrial facility and 122 samples from slaughtered pigs raised in backyard system were collected. Isolation and identification of S. suis were conducted using standard bacteriological methods. Farm management and risk factor data were collected by a questionnaire. Serotyping and presence of virulence factor genes, epf, mrp and sly, were determined by multiplex PCR assay. The overall incidence of S. suis in this study was 9% (n = 212) and the incidence is significantly higher in districts located at a greater distance south of Chiang Mai City. S. suis serotype 2 was present more in healthy pigs (43%) than ill pigs (10%). Every S. suis isolate carried mrp and sly and ill pigs carried epf (80%) more than healthy pigs (57%). However, the probability of S. suis serotype 2 with epf+ (0.245) detected in healthy pigs was higher than in ill pigs (0.08) indicating people may have a higher risk of being infected with S. suis from healthy than ill pigs.

  7. Trichuris suis and Oesophagostomum dentatum show different sensitivity and accumulation of fenbendazole, albendazole and levamisole in vitro.

    Directory of Open Access Journals (Sweden)

    Tina V A Hansen

    2014-04-01

    Full Text Available BACKGROUND: The single-dose benzimidazoles used against Trichuris trichiura infections in humans are not satisfactory. Likewise, the benzimidazole, fenbendazole, has varied efficacy against Trichuris suis whereas Oesophagostomum dentatum is highly sensitive to the drug. The reasons for low treatment efficacy of Trichuris spp. infections are not known. METHODOLOGY: We studied the effect of fenbendazole, albendazole and levamisole on the motility of T. suis and O. dentatum and measured concentrations of the parent drug compounds and metabolites of the benzimidazoles within worms in vitro. The motility and concentrations of drug compounds within worms were compared between species and the maximum specific binding capacity (Bmax of T. suis and O. dentatum towards the benzimidazoles was estimated. Comparisons of drug uptake in living and killed worms were made for both species. PRINCIPAL FINDINGS: The motility of T. suis was generally less decreased than the motility of O. dentatum when incubated in benzimidazoles, but was more decreased when incubated in levamisole. The Bmax were significantly lower for T. suis (106.6, and 612.7 pmol/mg dry worm tissue than O. dentatum (395.2, 958.1 pmol/mg dry worm tissue when incubated for 72 hours in fenbendazole and albendazole respectively. The total drug concentrations (pmol/mg dry worm tissue were significantly lower within T. suis than O. dentatum whether killed or alive when incubated in all tested drugs (except in living worms exposed to fenbendazole. Relatively high proportions of the anthelmintic inactive metabolite fenbendazole sulphone was measured within T. suis (6-17.2% as compared to O. dentatum (0.8-0.9%. CONCLUSION/SIGNIFICANCE: The general lower sensitivity of T. suis towards BZs in vitro seems to be related to a lower drug uptake. Furthermore, the relatively high occurrence of fenbendazole sulphone suggests a higher detoxifying capacity of T. suis as compared to O. dentatum.

  8. Trichuris suis and Oesophagostomum dentatum show different sensitivity and accumulation of fenbendazole, albendazole and levamisole in vitro.

    Science.gov (United States)

    Hansen, Tina V A; Nejsum, Peter; Friis, Christian; Olsen, Annette; Thamsborg, Stig Milan

    2014-04-01

    The single-dose benzimidazoles used against Trichuris trichiura infections in humans are not satisfactory. Likewise, the benzimidazole, fenbendazole, has varied efficacy against Trichuris suis whereas Oesophagostomum dentatum is highly sensitive to the drug. The reasons for low treatment efficacy of Trichuris spp. infections are not known. We studied the effect of fenbendazole, albendazole and levamisole on the motility of T. suis and O. dentatum and measured concentrations of the parent drug compounds and metabolites of the benzimidazoles within worms in vitro. The motility and concentrations of drug compounds within worms were compared between species and the maximum specific binding capacity (Bmax) of T. suis and O. dentatum towards the benzimidazoles was estimated. Comparisons of drug uptake in living and killed worms were made for both species. The motility of T. suis was generally less decreased than the motility of O. dentatum when incubated in benzimidazoles, but was more decreased when incubated in levamisole. The Bmax were significantly lower for T. suis (106.6, and 612.7 pmol/mg dry worm tissue) than O. dentatum (395.2, 958.1 pmol/mg dry worm tissue) when incubated for 72 hours in fenbendazole and albendazole respectively. The total drug concentrations (pmol/mg dry worm tissue) were significantly lower within T. suis than O. dentatum whether killed or alive when incubated in all tested drugs (except in living worms exposed to fenbendazole). Relatively high proportions of the anthelmintic inactive metabolite fenbendazole sulphone was measured within T. suis (6-17.2%) as compared to O. dentatum (0.8-0.9%). The general lower sensitivity of T. suis towards BZs in vitro seems to be related to a lower drug uptake. Furthermore, the relatively high occurrence of fenbendazole sulphone suggests a higher detoxifying capacity of T. suis as compared to O. dentatum.

  9. Symptoms after ingestion of pig whipworm Trichuris suis eggs in a randomized placebo-controlled double-blind clinical trial

    DEFF Research Database (Denmark)

    Bager, Peter; Kapel, Christian Moliin Outzen; Roepstorff, Allan Knud

    2011-01-01

    Symptoms after human infection with the helminth Trichuris suis have not previously been described. Exposure to helminths has been suggested as immune therapy against allergy and autoimmune diseases. We randomized adults with allergic rhinitis to ingest a dose of 2500 T. suis eggs or placebo every...... by a fluoroenzymeimmunoassay (Phadia ApS). During 163 days complete follow-up, subjects ingesting T. suis eggs (N = 49) had a three to 19-fold higher rate of events (median duration, 2 days) with gastrointestinal reactions (moderate to severe flatulence, diarrhea, and upper abdominal pain) compared with placebo subjects (N...... of episodes with onset before day 42 was ≤ 14 days in 80% of affected subjects. Age, gender, total IgE, and recent intestinal symptoms at baseline did not predict gastrointestinal side effects. In conclusion, during the first 2 months, repeated ingestions of 2500 T. suis eggs caused frequent gastrointestinal...

  10. Análise temporal dos setores de aglomerados subnormais dos censos 2000 e 2010: o estudo de caso da subprefeitura de São Mateus no município de São Paulo-SP

    OpenAIRE

    Alexandra Aguiar Pedro

    2016-01-01

    O propósito deste trabalho é investigar as características dos dados sobre aglomerados subnormais, na subprefeitura de São Mateus - município de São Paulo - SP, tendo em vista a realização de análises temporais. Os censos demográficos, por meio dos setores de aglomerados subnormais, são uma importante fonte de dados socioeconômicos e demográficos sobre favelas, coletados periodicamente e disponibilizados pelo IBGE para todos os municípios brasileiros. No entanto, pesquisadores e poder público...

  11. Virulence genes and genetic diversity of Streptococcus suis serotype 2 isolates from Thailand.

    Science.gov (United States)

    Maneerat, K; Yongkiettrakul, S; Kramomtong, I; Tongtawe, P; Tapchaisri, P; Luangsuk, P; Chaicumpa, W; Gottschalk, M; Srimanote, P

    2013-11-01

    Isolates of Streptococcus suis from different Western countries as well as those from China and Vietnam have been previously well characterized. So far, the genetic characteristics and relationship between S. suis strains isolated from both humans and pigs in Thailand are unknown. In this study, a total of 245 S. suis isolates were collected from both human cases (epidemic and sporadic) and pigs (diseased and asymptomatic) in Thailand. Bacterial strains were identified by biochemical tests and PCR targeting both, the 16S rRNA and gdh genes. Thirty-six isolates were identified as serotype 2 based on serotyping and the cps2-PCR. These isolates were tested for the presence of six virulence-associated genes: an arginine deiminase (arcA), a 38-kDa protein and protective antigen (bay046), an extracellular factor (epf), an hyaluronidase (hyl), a muramidase-released protein (mrp) and a suilysin (sly). In addition, the genetic diversities of these isolates were studied by RAPD PCR and multilocus sequence typing (MLST) analysis. Four virulence-associated gene patterns (VAGP 1 to 4) were obtained, and the majority of isolates (32/36) carried all genes tested (VAGP1). Each of the three OPB primers used provided 4 patterns designated RAPD-A to RAPD-D. Furthermore, MLST analysis could also distinguish the 36 isolates into four sequence types (STs): ST1 (n = 32), ST104 (n = 2), ST233 (n = 1) and a newly identified ST, ST336 (n = 1). Dendrogram constructions based on RAPD patterns indicated that S. suis serotype 2 isolates from Thailand could be divided into four groups and that the characteristics of the individual groups were in complete agreement with the virulence gene profiles and STs. The majority (32/36) of isolates recovered from diseased pigs, slaughterhouse pigs or human patients could be classified into a single group (VAGP1, RAPD-A and ST1). This genetic information strongly suggests the transmission of S. suis isolates from pigs to humans in Thailand. Our findings are

  12. The cell envelope subtilisin-like proteinase is a virulence determinant for Streptococcus suis

    Directory of Open Access Journals (Sweden)

    Gottschalk Marcelo

    2010-02-01

    Full Text Available Abstract Background Streptococcus suis is a major swine pathogen and zoonotic agent that mainly causes septicemia, meningitis, and endocarditis. It has recently been suggested that proteinases produced by S. suis (serotype 2 are potential virulence determinants. In the present study, we screened a S. suis mutant library created by the insertion of Tn917 transposon in order to isolate a mutant deficient in a cell surface proteinase. We characterized the gene and assessed the proteinase for its potential as a virulence factor. Results Two mutants (G6G and M3G possessing a single Tn917 insertion were isolated. The affected gene coded for a protein (SSU0757 that shared a high degree of identity with Streptococccus thermophilus PrtS (95.9% and, to a lesser extent, with Streptococcus agalactiae CspA (49.5%, which are cell surface serine proteinases. The SSU0757 protein had a calculated molecular mass of 169.6 kDa and contained the catalytic triad characteristic of subtilisin family proteinases: motif I (Asp200, motif II (His239, and motif III (Ser568. SSU0757 also had the Gram-positive cell wall anchoring motif (Leu-Pro-X-Thr-Gly at the carboxy-terminus, which was followed by a hydrophobic domain. All the S. suis isolates tested, which belonged to different serotypes, possessed the gene encoding the SSU0757 protein. The two mutants devoid of subtilisin-like proteinase activity had longer generation times and were more susceptible to killing by whole blood than the wild-type parent strain P1/7. The virulence of the G6G and M3G mutants was compared to the wild-type strain in the CD1 mouse model. Significant differences in mortality rates were noted between the P1/7 group and the M3G and G6G groups (p Conclusion In summary, we identified a gene coding for a cell surface subtilisin-like serine proteinase that is widely distributed in S. suis. Evidences were brought for the involvement of this proteinase in S. suis virulence.

  13. Prevalence of multiple drug resistant Streptococcus suis in and around Guwahati, India

    Directory of Open Access Journals (Sweden)

    Mrinalee Devi

    2017-05-01

    Full Text Available Aim: This study was conducted to determine the prevalence and antimicrobial susceptibility of Streptococcus suis and their resistance patterns isolated from both clinically healthy carriers and diseased pigs in and around Guwahati, Assam, India. Materials and Methods: A total of 497 samples were collected during October, 2012, to April, 2014, from clinically healthy (n=67 and diseased (n=230 pigs of varying age and either sex maintained under organized and unorganized farming systems. Samples were processed for isolation and identification of S. suis by biochemical characterization and polymerase chain reaction targeting the housekeeping gene glutamate dehydrogenase. In vitro antimicrobial susceptibility of the recovered isolates against nine antibiotic groups comprising 17 antimicrobial agents was studied by standard method. Results: Of the 497 samples examined, 7 (1.41% isolates were confirmed to be S. suis of which 5 (1.87% and 2 (0.87% were derived from clinically healthy and diseased pigs, respectively. All the isolates were susceptible to gentamicin, amikacin, and erythromycin (100% followed by the penicillin group and enrofloxacin (85.71%, ceftriaxone, doxycycline HCL, ofloxacin and chloramphenicol (71.43%, to kanamycin, clindamycin and co-trimoxazole (42.85%. The isolates showed least susceptibility to cefalexin, tetracycline and streptomycin (28.57%. All the five S. suis isolates from clinically healthy pigs were susceptible to penicillin G, amoxyclav, doxycycline HCl, gentamicin, amikacin and erythromycin, 80.00% isolates susceptible to ampicillin, enrofloxacin and ofloxacin, 60.00% to ceftriaxone, kanamycin and chloramphenicol, 40% to cefalexin, tetracycline, clindamycin and co-trimoxazole, respectively. Only 20.00% isolates were susceptible to streptomycin. Both the isolates recovered from diseased pigs were susceptible to ampicillin, ceftriaxone, gentamicin, amikacin, enrofloxacin, erythromycin, and clindamycin. On the other hand

  14. PK-PD Integration Modeling and Cutoff Value of Florfenicol against Streptococcus suis in Pigs

    Directory of Open Access Journals (Sweden)

    Zhixin Lei

    2018-01-01

    Full Text Available The aims of the present study were to establish optimal doses and provide an alternate COPD for florfenicol against Streptococcus suis based on pharmacokinetic-pharmacodynamic integration modeling. The recommended dose (30 mg/kg b.w. were administered in healthy pigs through intramuscular and intravenous routes for pharmacokinetic studies. The main pharmacokinetic parameters of Cmax, AUC0-24h, AUC, Ke, t1/2ke, MRT, Tmax, and Clb, were estimated as 4.44 μg/ml, 88.85 μg⋅h/ml, 158.56 μg⋅h/ml, 0.048 h-1, 14.46 h, 26.11 h, 4 h and 0.185 L/h⋅kg, respectively. The bioavailability of florfenicol was calculated to be 99.14% after I.M administration. A total of 124 Streptococcus suis from most cities of China were isolated to determine the minimum inhibitory concentration (MIC of florfenicol. The MIC50 and MIC90 were calculated as 1 and 2 μg/ml. A serotype 2 Streptococcus suis (WH-2, with MIC value similar to MIC90, was selected as a representative for an in vitro and ex vivo pharmacodynamics study. The MIC values of WH-2 in TSB and plasma were 2 μg/ml, and the MBC/MIC ratios were 2 in TSB and plasma. The MPC was detected to be 3.2 μg/ml. According to inhibitory sigmoid Emax model, plasma AUC0-24h/MIC values of florfenicol versus Streptococcus suis were 37.89, 44.02, and 46.42 h for the bactericidal, bacteriostatic, and elimination activity, respectively. Monte Carlo simulations the optimal doses for bactericidal, bacteriostatic, and elimination effects were calculated as 16.5, 19.17, and 20.14 mg/kg b.w. for 50% target attainment rates (TAR, and 21.55, 25.02, and 26.85 mg/kg b.w. for 90% TAR, respectively. The PK-PD cutoff value (COPD analyzed from MCS for florfenicol against Streptococcus suis was 1 μg/ml which could provide a sensitivity cutoff value. These results contributed an optimized alternative to clinical veterinary medicine and showed that the dose of 25.02 mg/kg florfenicol for 24 h could have a bactericidal action against

  15. Brucella suis vaccine strain 2 induces endoplasmic reticulum stress that affects intracellular replication in goat trophoblast cells in vitro

    Directory of Open Access Journals (Sweden)

    Xiangguo eWang

    2016-02-01

    Full Text Available Brucella has been reported to impair placental trophoblasts, a cellular target where Brucella efficiently replicates in association with the endoplasmic reticulum (ER, and ultimately trigger abortion in pregnant animals. However, the precise effects of Brucella on trophoblast cells remain unclear. Here, we describe the infection and replication of Brucella suis vaccine strain 2 (B.suis.S2 in goat trophoblast cells (GTCs and the cellular and molecular responses induced in vitro. Our studies demonstrated that B.suis.S2 was able to infect and proliferate to high titers, hamper the proliferation of GTCs and induce apoptosis due to ER stress. Tunicamycin (Tm, a pharmacological chaperone that strongly mounts ER stress-induced apoptosis, inhibited B.suis.S2 replication in GTCs. In addition, 4 phenyl butyric acid (4-PBA, a pharmacological chaperone that alleviates ER stress-induced apoptosis, significantly enhanced B.suis.S2 replication in GTCs. The Unfolded Protein Response (UPR chaperone molecule GRP78 also promoted B.suis.S2 proliferation in GTCs by inhibiting ER stress-induced apoptosis. We also discovered that the IRE1 pathway, but not the PERK or ATF6 pathway, was activated in the process. However, decreasing the expression of phosphoIRE1α and IRE1α proteins with Irestatin 9389 (IRE1 antagonist in GTCs did not affect the proliferation of B.suis.S2. Although GTC implantation was not affected upon B.suis.S2 infection, progesterone secretion was suppressed, and prolactin and estrogen secretion increased; these effects were accompanied by changes in the expression of genes encoding key steroidogenic enzymes. This study systematically explored the mechanisms of abortion in Brucella infection from the viewpoint of pathogen invasion, ER stress and reproductive endocrinology. Our findings may provide new insight for understanding the mechanisms involved in goat abortions caused by Brucella infection.

  16. Mycoplasma suis infection results endothelial cell damage and activation: new insight into the cell tropism and pathogenicity of hemotrophic mycoplasma

    Directory of Open Access Journals (Sweden)

    Sokoli Albina

    2013-02-01

    Full Text Available Abstract Hemotrophic mycoplasmas (HM are highly specialized red blood cell parasites that cause infectious anemia in a variety of mammals, including humans. To date, no in vitro cultivation systems for HM have been available, resulting in relatively little information about the pathogenesis of HM infection. In pigs, Mycoplasma suis-induced infectious anemia is associated with hemorrhagic diathesis, and coagulation dysfunction. However, intravasal coagulation and subsequent consumption coagulopathy can only partly explain the sequence of events leading to hemorrhagic diathesis manifesting as cyanosis, petechial bleeding, and ecchymosis, and to disseminated coagulation. The involvement of endothelial activation and damage in M. suis-associated pathogenesis was investigated using light and electron microscopy, immunohistochemistry, and cell sorting. M. suis interacted directly with endothelial cells in vitro and in vivo. Endothelial activation, widespread endothelial damage, and adherence of red blood cells to the endothelium were evident in M. suis-infected pigs. These alterations of the endothelium were accompanied by hemorrhage, intravascular coagulation, vascular occlusion, and massive morphological changes within the parenchyma. M. suis biofilm-like microcolonies formed on the surface of endothelial cells, and may represent a putative persistence mechanism of M. suis. In vitro analysis demonstrated that M. suis interacted with the endothelial cytoskeletal protein actin, and induced actin condensation and activation of endothelial cells, as determined by the up-regulation of ICAM, PECAM, E-selectin, and P-selectin. These findings demonstrate an additional cell tropism of HM for endothelial cells and suggest that M. suis interferes with the protective function of the endothelium, resulting in hemorrhagic diathesis.

  17. Lysogenic Streptococcus suis isolate SS2-4 containing prophage SMP showed increased mortality in zebra fish compared to the wild-type isolate.

    Directory of Open Access Journals (Sweden)

    Fang Tang

    Full Text Available Streptococcus suis (S. suis infection is considered to be a major problem in the swine industry worldwide. Based on the capsular type, 33 serotypes of S. suis have been described, with serotype 2 (SS2 being the most frequently isolated from diseased piglets. Little is known, however, about the pathogenesis and virulence factors of S. suis. Research on bacteriophages highlights a new area in S. suis research. A S. suis serotype 2 bacteriophage, designated SMP, has been previously isolated in our laboratory. Here, we selected a lysogenic isolate in which the SMP phage was integrated into the chromosome of strain SS2-4. Compared to the wild-type isolate, the lysogenic strain showed increased mortality in zebra fish. Moreover the sensitivity of the lysogenic strain to lysozyme was seven times higher than that of the wild-type.

  18. A genetic analysis of Trichuris trichiura and Trichuris suis from Ecuador

    DEFF Research Database (Denmark)

    Meekums, Hayley; Hawash, Mohamed B F; Sparks, Alexandra M

    2015-01-01

    BACKGROUND: Since the nematodes Trichuris trichiura and T. suis are morphologically indistinguishable, genetic analysis is required to assess epidemiological cross-over between people and pigs. This study aimed to clarify the transmission biology of trichuriasis in Ecuador. FINDINGS: Adult...... Trichuris worms were collected during a parasitological survey of 132 people and 46 pigs in Esmeraldas Province, Ecuador. Morphometric analysis of 49 pig worms and 64 human worms revealed significant variation. In discriminant analysis morphometric characteristics correctly classified male worms according...... to genetically analyse Trichuris parasites. Although T. trichiura does not appear to be zoonotic in Ecuador, there is evidence of genetic exchange between T. trichiura and T. suis warranting more detailed genetic sampling....

  19. Glucose 6-phosphate dehydrogenase: isoenzymatic pattern in Oesophagostomum venulosum, Trichuris ovis and T. suis.

    Science.gov (United States)

    Rodriguez, B; Cutillas, C; German, P; Guevara, D

    1991-12-01

    In the present communication we have studied the isoenzymatic pattern activity of the glucose 6-phosphate dehydrogenase (G6PD) in Oesophagostomum venulosum, Trichuris ovis and T. suis, parasites of Capra hircus (goat), Ovis aries (sheep) and Sus scrofa domestica (pig) respectively, by polyacrylamide gel electrophoresis. Different phenotypes have been observed in the G6PD isoenzymatic pattern activity in males and females of Oesophagostomum venulosum. Furthermore, G6PD activity has been assayed in Trichuris ovis collected from Ovis aries and Capra hircus. No differences have been observed in the isoenzymatic patterns attending to the different hosts. All the individuals exhibited one single band or two bands; this suggests a monomeric condition for G6PD in T. ovis. In T. suis the enzyme G6PD appeared as a single electrophoretic band in about 85.7% of the individuals.

  20. Trichurs suis ova theraphy in relapsing multiple sclerosis is safe but without signals of beneficial effect

    DEFF Research Database (Denmark)

    Voldsgaard, A.; Bager, P.; Garde, E.

    2015-01-01

    revealed 6 new or enlarged T2 lesions in the run-in period, 7 lesions in the early period and 21 lesions in the late treatment period. Two patients suffered a relapse before treatment and two during treatment. Eight patients developed eosinophilia. The expression of cytokines and transcription factors did...... not change. CONCLUSIONS: In a small group of relapsing multiple sclerosis patients, Trichuris suis oral therapy was well tolerated but without beneficial effect....

  1. Isolamento di Brucella suis biovariante 2 da un cinghiale in Abruzzo, Italia

    Directory of Open Access Journals (Sweden)

    Fabrizio De Massis

    2012-12-01

    Full Text Available Un cinghiale selvatico femmina, di circa due anni di età, è stato trovato morto dai Servizi Veterinari a Pianola di Roio a L'Aquila, Provincia situata nella Regione Abruzzo nell'Italia centrale. La carcassa è stata conferita all'Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale" di Teramo per l'esecuzione dell'esame autoptico. Dai linfonodi sotto-mandibolari di questo esemplare è stato isolato un ceppo di Brucella suis biovariante 2. Questa è la prima segnalazione d'isolamento di B. suis nella Regione Abruzzo. Diversi autori hanno accettato, in passato, l'ipotesi che B. suis biovariante 2 sia stata introdotta in Italia attraverso l'importazione di lepri provenienti dai paesi europei in cui l'infezione è endemica nelle popolazioni selvatiche. Questa considerazione ha portato le autorità italiane a rafforzare i controlli esistenti sulle lepri selvatiche importate a scopo di ripopolamento. Tuttavia, attualmente, non è in vigore alcuna disposizione (né lo è stata in passato per il controllo della brucellosi nei cinghiali movimentati, sia a livello nazionale che europeo. L'isolamento di B. suis biovariante 2 da cinghiali in altre Regioni italiane geograficamente distanti potrebbe suggerire che questa specie, piuttosto che le lepri importate, possa essere stata la fonte d'introduzione dell'infezione in tali aree. Le norme nazionali ed europee di gestione della brucellosi nella fauna selvatica dovrebbero essere indirizzate al controllo dello stato di salute dei cinghiali negli allevamenti prima delle movimentazioni o del rilascio, con l'obiettivo di prevenire la diffusione di questo patogeno in territori indenni.

  2. Marginal vitamin A deficiency in pigs experimentally infected with Trichuris suis

    DEFF Research Database (Denmark)

    Pedersen, S; Saeed, I; Jensen, S K

    2001-01-01

    were marginally smaller (36.7 vs 40.2 mm; P = 0.08), more orally located (section 2.9 vs 3.9; P = 0.08) and had a higher proportion of males (0.58 vs 0.50; P = 0.08) whereas there were no effects of diet treatment on fecundity. The proportion of pigs with faecal T. suis egg excretion 12 weeks post...

  3. Cryptosporidium suis and Cryptosporidium scrofarum in Eurasian wild boars (Sus scrofa) in Central Europe

    Czech Academy of Sciences Publication Activity Database

    Němejc, K.; Sak, Bohumil; Květoňová, Dana; Hanzal, V.; Janiszewski, P.; Forejtek, P.; Rajský, D.; Ravaszová, P.; McEvoy, J.; Kváč, Martin

    2013-01-01

    Roč. 197, 3-4 (2013), s. 504-508 ISSN 0304-4017 Grant - others:Jihočeská univerzita(CZ) 022/2010/Z; Jihočeská univerzita(CZ) 11/2013/Z Institutional support: RVO:60077344 Keywords : Central Europe * Cryptosporidium scrofarum * Cryptosporidium suis * Eurasian wild boar * PCR * SSU Subject RIV: EE - Microbiology, Virology Impact factor: 2.545, year: 2013

  4. The arginine-ornithine antiporter ArcD contributes to biological fitness of Streptococcus suis

    Directory of Open Access Journals (Sweden)

    Marcus eFulde

    2014-08-01

    Full Text Available The arginine-ornithine antiporter (ArcD is part of the Arginine Deiminase System (ADS, a catabolic, energy-providing pathway found in a variety of different bacterial species, including the porcine zoonotic pathogen Streptococcus suis. The ADS has recently been shown to play a role in the pathogenicity of S. suis, in particular in its survival in host cells. The contribution of arginine and arginine transport mediated by ArcD, however, has yet to be clarified. In the present study, we showed by experiments using [U-13C6]arginine as a tracer molecule that S. suis is auxotrophic for arginine and that bacterial growth depends on the uptake of extracellular arginine. To further study the role of ArcD in arginine metabolism, we generated an arcD-specific mutant strain and characterized its growth compared to the wild-type (WT strain, a virulent serotype 2 strain. The mutant strain showed a markedly reduced growth rate in chemically defined media supplemented with arginine when compared to the WT strain, indicating that ArcD promotes arginine uptake. To further evaluate the in vivo relevance of ArcD, we studied the intracellular bacterial survival of the arcD mutant strain in an epithelial cell culture infection model. The mutant strain was substantially attenuated, and its reduced intracellular survival rate correlated with a lower ability to neutralize the acidified environment. Based on these results, we propose that ArcD, by its function as an arginine-ornithine antiporter, is important for supplying arginine as substrate of the ADS and, thereby, contributes to biological fitness and virulence of S. suis in the host.

  5. Una anatomía de la población colombiana: la técnica estadística en Colombia y el levantamiento del censo de población en 1912

    Directory of Open Access Journals (Sweden)

    Fabián Prieto

    2005-12-01

    Full Text Available This article follows the incorporation of statistical techniques into Colombian State at the beginning of 20th century. Although classification categories are an interesting starting point, the study of the process that was made allows improving the purpose that these categories laid, mostly because this kind of project allows to perform them. The census publication of 1912 is taken as the principal source but its articulation is reconstructed with files documentation, statistical handbooks and other writings in order to follow the process of census making.//Este artículo busca rastrear la incorporación de la técnica estadística en el Estado Colombiano a comienzos del siglo XX. Si bien las categorías de clasificación ofrecen un punto de partida interesante, la revisión del proceso que fue llevada a cabo, permite refinar el alcance que estas categorías tuvieron, principalmente porque la puesta en marcha de un proyecto como éste termina por dar forma a las categorías la publicación del censo de 1912 es tomada como la fuente principal, pero su articulación es reconstruida con documentos de archivo, manuales de estadística y otros escritos con el fin de seguir el proceso de elaboración del censo.

  6. Pre-absorbed immunoproteomics: a novel method for the detection of Streptococcus suis surface proteins.

    Directory of Open Access Journals (Sweden)

    Wei Zhang

    Full Text Available Streptococcus suis serotype 2 (SS2 is a zoonotic pathogen that can cause infections in pigs and humans. Bacterial surface proteins are often investigated as potential vaccine candidates and biomarkers of virulence. In this study, a novel method for identifying bacterial surface proteins is presented, which combines immunoproteomic and immunoserologic techniques. Critical to the success of this new method is an improved procedure for generating two-dimensional electrophoresis gel profiles of S. suis proteins. The S. suis surface proteins identified in this study include muramidase-released protein precursor (MRP and an ABC transporter protein, while MRP is thought to be one of the main virulence factors in SS2 located on the bacterial surface. Herein, we demonstrate that the ABC transporter protein can bind to HEp-2 cells, which strongly suggests that this protein is located on the bacterial cell surface and may be involved in pathogenesis. An immunofluorescence assay confirmed that the ABC transporter is localized to the bacterial outer surface. This new method may prove to be a useful tool for identifying surface proteins, and aid in the development of new vaccine subunits and disease diagnostics.

  7. The porcine acute phase protein response to acute clinical and subclinical experimental infection with Streptococcus suis

    DEFF Research Database (Denmark)

    Sørensen, Nanna Skall; Tegtmeier, C.; Andresen, Lars Ole

    2006-01-01

    The pig acute phase protein (APP) response to experimental Streptococcus suis (S. suis) infection was mapped by the measurement of the positive APPs C-reactive protein (CRP), serum amyloid A (SAA), haptoglobin (Hp) and major acute phase protein (pig-MAP) and the negative APPs albumin...... and apolipoprotein (Apo) A-I. The aim was to elucidate the differences in the acute phase behaviour of the individual APPs during a typical bacterial septicaemic, infection. Pigs were inoculated subcutaneously with live S. suis serotype 2 and blood was sampled before and on various days post inoculation (p...... the experiment with maximum levels around 10 times the day 0-levels, and pig-MAP was elevated on days 1-12 p.i. with peak levels of around seven times the day 0-levels. Apo A-I was decreased from days 1 to 8 and showed minimum levels of about 40% of day 0-levels around 1-2 days p.i. No clear pattern of changes...

  8. Effect of Different Adjuvants on Protection and Side-Effects Induced by Helicobacter suis Whole-Cell Lysate Vaccination.

    Directory of Open Access Journals (Sweden)

    Iris Bosschem

    Full Text Available Helicobacter suis (H. suis is a widespread porcine gastric pathogen, which is also of zoonotic importance. The first goal of this study was to investigate the efficacy of several vaccine adjuvants (CpG-DNA, Curdlan, Freund's Complete and Incomplete, Cholera toxin, administered either subcutaneously or intranasally along with H. suis whole-cell lysate, to protect against subsequent H. suis challenge in a BALB/c infection model. Subcutaneous immunization with Freund's complete (FC/lysate and intranasal immunization with Cholera toxin (CT/lysate were shown to be the best options for vaccination against H. suis, as determined by the amount of colonizing H. suis bacteria in the stomach, although adverse effects such as post-immunization gastritis/pseudo-pyloric metaplasia and increased mortality were observed, respectively. Therefore, we decided to test alternative strategies, including sublingual vaccine administration, to reduce the unwanted side-effects. A CCR4 antagonist that transiently inhibits the migration of regulatory T cells was also included as a new adjuvant in this second study. Results confirmed that immunization with CT (intranasally or sublingually is among the most effective vaccination protocols, but increased mortality was still observed. In the groups immunized subcutaneously with FC/lysate and CCR4 antagonist/lysate, a significant protection was observed. Compared to the FC/lysate immunized group, gastric pseudo-pyloric metaplasia was less severe or even absent in the CCR4 antagonist/lysate immunized group. In general, an inverse correlation was observed between IFN-γ, IL-4, IL-17, KC, MIP-2 and LIX mRNA expression and H. suis colonization density, whereas lower IL-10 expression levels were observed in partially protected animals.

  9. Slaughterhouse pigs are a major reservoir of Streptococcus suis serotype 2 capable of causing human infection in southern Vietnam.

    Directory of Open Access Journals (Sweden)

    Thi Hoa Ngo

    2011-03-01

    Full Text Available Streptococcus suis is a pathogen of major economic significance to the swine industry and is increasingly recognized as an emerging zoonotic agent in Asia. In Vietnam, S. suis is the leading cause of bacterial meningitis in adult humans. Zoonotic transmission is most frequently associated with serotype 2 strains and occupational exposure to pigs or consumption of infected pork. To gain insight into the role of pigs for human consumption as a reservoir for zoonotic infection in southern Vietnam, we determined the prevalence and diversity of S. suis carriage in healthy slaughterhouse pigs. Nasopharyngeal tonsils were sampled from pigs at slaughterhouses serving six provinces in southern Vietnam and Ho Chi Minh City area from September 2006 to November 2007. Samples were screened by bacterial culture. Isolates of S. suis were serotyped and characterized by multi locus sequence typing (MLST and pulse field gel electrophoresis (PFGE. Antibiotic susceptibility profiles and associated genetic resistance determinants, and the presence of putative virulence factors were determined. 41% (222/542 of pigs carried S. suis of one or multiple serotypes. 8% (45/542 carried S. suis serotype 2 which was the most common serotype found (45/317 strains, 14%. 80% of serotype 2 strains belonged to the MLST clonal complex 1,which was previously associated with meningitis cases in Vietnam and outbreaks of severe disease in China in 1998 and 2005. These strains clustered with representative strains isolated from patients with meningitis in PFGE analysis, and showed similar antimicrobial resistance and virulence factor profiles. Slaughterhouse pigs are a major reservoir of S. suis serotype 2 capable of causing human infection in southern Vietnam. Strict hygiene at processing facilities, and health education programs addressing food safety and proper handling of pork should be encouraged.

  10. Diagnosis Infeksi Streptococcus suis serotipe-2 pada Babi Secara Serologi dengan Muramidase Released Protein (SEROLOGICALLY DIAGNOSE OF STREPTOCOCCUS SUIS SEROTYPE-2 INFECTION IN PIGS BASED ON MURAMIDASE RELEASED PROTEIN

    Directory of Open Access Journals (Sweden)

    Siti Isrina Oktavia Salasia

    2016-01-01

    Full Text Available Streptococcus suis is a bacterial pathogen causing disease of pigs that characterized by meningitis,bronchopneumonia, arthritis, pericarditis, polyserositis and septicaemia. S. suis especially serotype 2 caninfect human (zoonotic with a special symptom of meningitis. The aim of this research was to detect S.suis infection based on muramidase released protein (MRP, as an important virulence marker of S. suis.S. suis serotype 2 strain P171 with phenotype of MRP+EF+ was used in this research. The MRP antigen wasextracted using lysozyme and separated by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE. Balb/c mice were imunized with 136 kDa MRP to produce antibody against MRP. Theantibody was evaluated by using enzyme linkage immunosorbent assay (ELISA. The results of the researchshowed that the antibody against MRP with molecular weight of 136 kDa could be produced on Balb/Cmice with the highest absorbance of 3,889 and could be used to detect field sera from infected pigs with200x dilution using ELISA antigen capture. Antibody against MRP could detect serologically of S. suisinfection in pigs in Papua with 50% seropositivy by using ELISA antigen capture and 40% by using dot blot.

  11. Análise de paradados do Censo Demográfico 2010: uma investigação de fatores associados a erros não amostrais do levantamento de dados

    Directory of Open Access Journals (Sweden)

    Luciano Tavares Duarte

    Full Text Available Resumo A relevância de um Censo Demográfico para o sistema de estatísticas públicas de uma nação é indiscutível do ponto de vista de sua abrangência temática e territorial. Em contrapartida, sua complexidade e dimensão levam a desafios na garantia da qualidade de seus resultados. O presente artigo tem por objetivo apresentar os possíveis fatores associados a erros não amostrais detectados na coleta das informações, mediante a análise de paradados e dos microdados do Censo Demográfico brasileiro de 2010. Os dados utilizados provêm das informações sobre a operação de coleta e administração da pesquisa oriundas, respectivamente, do sistema de gerenciamento de recursos humanos do pessoal de coleta e do sistema de supervisão da operação de coleta, ou seja, os paradados. Também se utilizam os microdados do universo do Censo Demográfico. Neste estudo foram analisadas as divergências observadas entre as informações coletadas pelos recenseadores e aquelas obtidas por supervisores nas reentrevistas realizadas em procedimentos de supervisão do trabalho de campo. Para análise de divergências detectadas entre os dados coletados por recenseadores e supervisores, foram empregados modelos hierárquicos generalizados. Os resultados mostram que existem diferenciais nas divergências associados à estrutura de coleta dos dados e às características dos recenseadores, supervisores e informantes, além de revelarem diferenças regionais. Fica evidente, sobretudo, uma forte influência das características do informante nas chances de ocorrência das divergências, em detrimento das características dos supervisores e recenseadores. Os resultados da modelagem estatística sugerem que as entrevistas realizadas com informantes do sexo masculino, analfabetos ou com baixa escolaridade, mais velhos e que vivem em domicílios com indicadores que refletem condições de vida menos satisfatórias apresentam aumento nas chances em favor da

  12. Postantibiotic effects and postantibiotic sub-MIC effects of tilmicosin, erythromycin and tiamulin on erythromycin-resistant Streptococcus suis

    Directory of Open Access Journals (Sweden)

    Liping Wang

    2009-12-01

    Full Text Available The postantibiotic effects (PAEs and postantibiotic sub-MIC effects (PA SMEs of tilmicosin, erythromycin and tiamulin on erythromycin-susceptible and erythromycin-resistant strains of Streptococcus suis (M phenotype were investigated in vitro. Tilmicosin and tiamulin induced significantly longer PAE and PA SME against both erythromycin-susceptible and erythromycin-resistant strains than did erythromycin. The durations of PAE and PA SMEs were proportional to the concentrations of drugs used for exposure. The PA SMEs were substantially longer than PAEs on S. suis (P<0.05 regardless of the antimicrobial used for exposure. The results indicated that the PAE and PA SME could help in the design of efficient control strategies for infection especially caused by erythromycin-resistant S. suis and that they may provide additional valuable information for the rational drug use in clinical practice.

  13. Postantibiotic effects and postantibiotic sub-MIC effects of tilmicosin, erythromycin and tiamulin on erythromycin-resistant Streptococcus suis.

    Science.gov (United States)

    Wang, Liping; Zhang, Yuanshu

    2009-10-01

    The postantibiotic effects (PAEs) and postantibiotic sub-MIC effects (PA SMEs) of tilmicosin, erythromycin and tiamulin on erythromycin-susceptible and erythromycin-resistant strains of Streptococcus suis (M phenotype) were investigated in vitro. Tilmicosin and tiamulin induced significantly longer PAE and PA SME against both erythromycin-susceptible and erythromycin-resistant strains than did erythromycin. The durations of PAE and PA SMEs were proportional to the concentrations of drugs used for exposure. The PA SMEs were substantially longer than PAEs on S. suis (P<0.05) regardless of the antimicrobial used for exposure. The results indicated that the PAE and PA SME could help in the design of efficient control strategies for infection especially caused by erythromycin-resistant S. suis and that they may provide additional valuable information for the rational drug use in clinical practice.

  14. Excreted/secreted Trichuris suis products reduce barrier function and suppress inflammatory cytokine production of intestinal epithelial cells

    DEFF Research Database (Denmark)

    Hiemstra, I. H.; Klaver, E. J.; Vrijland, K.

    2014-01-01

    The administration of helminths is considered a promising strategy for the treatment of autoimmune diseases due to their immunomodulatory properties. Currently, the application of the helminth Trichuris suis as a treatment for Crohn's disease is being studied in large multi-center clinical trials....... The intestinal epithelium forms an efficient barrier between the intestinal lumen containing the microbial flora and helminths, and dendritic cells (DCs) present in the lamina propria that determine the TH response. Here, we investigated how excreted/secreted (E/S) products of T. suis affect the barrier function...... of intestinal epithelial cells (IECs) in order to reach the DCs and modulate the immune response. We show that T. suis E/S products reduce the barrier function and the expression of the tight junction proteins EMP-1 and claudin-4 in IEC CMT93/69 monolayers in a glycan-dependent manner. This resulted...

  15. Clinical management of Brucella suis infection in dogs and implications for public health.

    Science.gov (United States)

    James, D R; Golovsky, G; Thornton, J M; Goodchild, L; Havlicek, M; Martin, P; Krockenberger, M B; Marriott, Dje; Ahuja, V; Malik, R; Mor, S M

    2017-01-01

    Brucellosis caused by Brucella suis is a notifiable disease that has recently emerged in dogs in New South Wales (NSW). Given the potential for zoonotic transmission, euthanasia of affected dogs is recommended, but this action is not mandatory. We report the clinical management of three dogs that underwent treatment at their owners' request. A 14-month-old spayed female crossbreed originally obtained from an urban animal shelter underwent extensive investigations in 2011-12 for lameness and back pain, culminating in decompressive laminectomy. Diagnosis of multifocal discospondylitis and spinal empyema was made, with B. suis cultured from surgical biopsy specimens. The dog responded to long-term treatment using rifampicin and doxycycline. A second case of B. suis infection was diagnosed in January 2016 in a 3-year-old crossbreed pig-hunting dog with unilateral testicular enlargement. Following serological diagnosis the dog was given preliminary therapy using rifampicin and doxycycline, the affected testis was resected and the patient given a further month of combination therapy. In March 2016 a 7-year-old crossbreed pig-hunting dog with brucellosis was handled similarly, although both testes were removed. Brucellosis should be considered in the differential diagnosis of back pain, discospondylitis, lameness, abortion, prostatic abscessation and testicular/epididymal enlargement in dogs, especially if there is exposure to feral pigs or consumption of uncooked feral pig meat. Euthanasia is the only guarantee of reducing the public health risk to zero. However, where treatment is desired by the owner, combination therapy using rifampicin and doxycycline appears to be effective, when combined with surgical resection of infected tissues. Further monitoring of dogs during and after treatment is required to document cure. © 2017 Australian Veterinary Association.

  16. The Surface-Exposed Protein SntA Contributes to Complement Evasion in Zoonotic Streptococcus suis.

    Science.gov (United States)

    Deng, Simin; Xu, Tong; Fang, Qiong; Yu, Lei; Zhu, Jiaqi; Chen, Long; Liu, Jiahui; Zhou, Rui

    2018-01-01

    Streptococcus suis is an emerging zoonotic pathogen causing streptococcal toxic shock like syndrome (STSLS), meningitis, septicemia, and even sudden death in human and pigs. Serious septicemia indicates this bacterium can evade the host complement surveillance. In our previous study, a functionally unknown protein SntA of S. suis has been identified as a heme-binding protein, and contributes to virulence in pigs. SntA can interact with the host antioxidant protein AOP2 and consequently inhibit its antioxidant activity. In the present study, SntA is identified as a cell wall anchored protein that functions as an important player in S. suis complement evasion. The C3 deposition and membrane attack complex (MAC) formation on the surface of sntA -deleted mutant strain Δ sntA are demonstrated to be significantly higher than the parental strain SC-19 and the complementary strain CΔ sntA . The abilities of anti-phagocytosis, survival in blood, and in vivo colonization of Δ sntA are obviously reduced. SntA can interact with C1q and inhibit hemolytic activity via the classical pathway. Complement activation assays reveal that SntA can also directly activate classical and lectin pathways, resulting in complement consumption. These two complement evasion strategies may be crucial for the pathogenesis of this zoonotic pathogen. Concerning that SntA is a bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase in many species of Gram-positive bacteria, these complement evasion strategies may have common biological significance.

  17. Brucella suis strain 2 vaccine is safe and protective against heterologous Brucella spp. infections.

    Science.gov (United States)

    Zhu, Liangquan; Feng, Yu; Zhang, Ge; Jiang, Hui; Zhang, Zhen; Wang, Nan; Ding, Jiabo; Suo, Xun

    2016-01-12

    Brucellosis is a wide spread zoonotic disease that causes abortion and infertility in mammals and leads to debilitating, febrile illness in humans. Brucella abortus, Brucella melitensis and Brucella suis are the major pathogenic species to humans. Vaccination with live attenuated B. suis strain 2 (S2) vaccine is an essential and critical component in the control of brucellosis in China. The S2 vaccine is very effective in preventing brucellosis in goats, sheep, cattle and swine. However, there are still debates outside of China whether the S2 vaccine is able to provide protection against heterologous virulent Brucella species. We investigated the residual virulence, immunogenicity and protective efficacy of the S2 vaccine in BALB/c mice by determining bacteria persistence in spleen, serum antibody response, cellular immune response and protection against a heterologous virulent challenge. The S2 vaccine was of low virulence as there were no bacteria recovered in spleen four weeks post vaccination. The vaccinated mice developed Brucella-specific IgG in 2-3 weeks, and a burst production of IFN-γ at one week as well as a two-fold increase in TNF-α production. The S2 vaccine protected mice from a virulent challenge by B. melitensis M28, B. abortus 2308 and B. suis S1330, and the S2 vaccinated mice did not develop any clinical signs or tissue damage. Our study demonstrated that the S2 vaccine is of low virulence, stimulates good humoral and cellular immunity and protects animals against infection by heterologous, virulent Brucella species. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. Susceptibility to antimicrobial agents of Streptococcus suis capsular type 2 strains isolated from pigs.

    Science.gov (United States)

    Seol, B; Kelneric, Z; Hajsig, D; Madic, J; Naglic, T

    1996-03-01

    The minimal inhibitory concentrations (MICs) for thirty-three epidemiologicaly unrelated clinical isolates of Streptococcus suis capsular type 2 were determined in relation to ampicillin, ampicillin-sulbactam, amoxicillin, clavulanate-amoxicillin, penicillin G, cephalexin, gentamicin, streptomycin, erythromycin, tylosin and doxycycline, using the microtitre broth dilution procedure described by the U.S. National Committee for Clinical Laboratory Standards (NCCLS). Gentamicin was the most active compound tested, with an MIC for 90% of the strains tested (MIC(90)) of 0.4 mg/L. Overall, 70% of strains were resistant to doxycycline (MIC(90) > or = 100.0 mg/L), followed by penicillin G (51% of strains) (MIC(90) + or = 100.0 mg/L). Resistance to amoxicillin and ampicillin was 36.4% (MIC(90) 12.5 mg/L) and 33.3% (MIC(90) 50.0 mg/L), respectively. 15.2% of S. suis strains were resistant to streptomycin, tylosin and cephalexin with MIC90 values of 25.0 mg/L, 12.5 mg/L and 25.0 mg/L, respectively. A combination of ampicillin and sulbactam (MIC(90) 6.3 mg/L) and a combination of amoxicillin and clavulanate (MIC(90) 3.1 mg/L) as well as erythromycin (1.6 mg/L) were of the same efficacy, with a total of 9.1% resistant S. suis strains. This high percentage of resistance to doxycycline and penicillin G precludes the use of these antibiotics as empiric therapy of swine diseases.

  19. Protection against Streptococcus suis Serotype 2 Infection Using a Capsular Polysaccharide Glycoconjugate Vaccine

    Science.gov (United States)

    Calzas, Cynthia; Shiao, Tze Chieh; Neubauer, Axel; Kempker, Jennifer; Roy, René; Gottschalk, Marcelo

    2016-01-01

    Streptococcus suis serotype 2 is an encapsulated bacterium and one of the most important bacterial pathogens in the porcine industry. Despite decades of research for an efficient vaccine, none is currently available. Based on the success achieved with other encapsulated pathogens, a glycoconjugate vaccine strategy was selected to elicit opsonizing anti-capsular polysaccharide (anti-CPS) IgG antibodies. In this work, glycoconjugate prototypes were prepared by coupling S. suis type 2 CPS to tetanus toxoid, and the immunological features of the postconjugation preparations were evaluated in vivo. In mice, experiments evaluating three different adjuvants showed that CpG oligodeoxyribonucleotide (ODN) induces very low levels of anti-CPS IgM antibodies, while the emulsifying adjuvants Stimune and TiterMax Gold both induced high levels of IgGs and IgM. Dose-response trials comparing free CPS with the conjugate vaccine showed that free CPS is nonimmunogenic independently of the dose used, while 25 μg of the conjugate preparation was optimal in inducing high levels of anti-CPS IgGs postboost. With an opsonophagocytosis assay using murine whole blood, sera from immunized mice showed functional activity. Finally, the conjugate vaccine showed immunogenicity and induced protection in a swine challenge model. When conjugated and administered with emulsifying adjuvants, S. suis type 2 CPS is able to induce potent IgM and isotype-switched IgGs in mice and pigs, yielding functional activity in vitro and protection against a lethal challenge in vivo, all features of a T cell-dependent response. This study represents a proof of concept for the potential of glycoconjugate vaccines in veterinary medicine applications against invasive bacterial infections. PMID:27113360

  20. A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates

    DEFF Research Database (Denmark)

    Chen, Chen; Tang, Jiaqi; Dong, Wei

    2007-01-01

    shock syndrome (STSS), which was originally associated with Streptococcus pyogenes (GAS) in Streptococci. However, the molecular mechanisms underlying STSS are poorly understood. METHODS AND FINDINGS: To elucidate the genetic determinants of STSS caused by SS2, whole genome sequencing of 3 different......BACKGROUND: Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen, causing more than 200 cases of severe human infection worldwide, with the hallmarks of meningitis, septicemia, arthritis, etc. Very recently, SS2 has been recognized as an etiological agent for streptococcal toxic...

  1. Evolution and genome specialization of Brucella suis biovar 2 Iberian lineages.

    Science.gov (United States)

    Ferreira, Ana Cristina; Tenreiro, Rogério; de Sá, Maria Inácia Corrêa; Dias, Ricardo

    2017-09-12

    Swine brucellosis caused by B. suis biovar 2 is an emergent disease in domestic pigs in Europe. The emergence of this pathogen has been linked to the increase of extensive pig farms and the high density of infected wild boars (Sus scrofa). In Portugal and Spain, the majority of strains share specific molecular characteristics, which allowed establishing an Iberian clonal lineage. However, several strains isolated from wild boars in the North-East region of Spain are similar to strains isolated in different Central European countries. Comparative analysis of five newly fully sequenced B. suis biovar 2 strains belonging to the main circulating clones in Iberian Peninsula, with publicly available Brucella spp. genomes, revealed that strains from Iberian clonal lineage share 74% similarity with those reference genomes. Besides the 210 kb translocation event present in all biovar 2 strains, an inversion with 944 kb was presented in chromosome I of strains from the Iberian clone. At left and right crossover points, the inversion disrupted a TRAP dicarboxylate transporter, DctM subunit, and an integral membrane protein TerC. The gene dctM is well conserved in Brucella spp. except in strains from the Iberian clonal lineage. Intraspecies comparative analysis also exposed a number of biovar-, haplotype- and strain-specific insertion-deletion (INDELs) events and single nucleotide polymorphisms (SNPs) that could explain differences in virulence and host specificities. Most discriminative mutations were associated to membrane related molecules (29%) and enzymes involved in catabolism processes (20%). Molecular identification of both B. suis biovar 2 clonal lineages could be easily achieved using the target-PCR procedures established in this work for the evaluated INDELs. Whole-genome analyses supports that the B. suis biovar 2 Iberian clonal lineage evolved from the Central-European lineage and suggests that the genomic specialization of this pathogen in the Iberian Peninsula

  2. Immune responses and parasitological observations induced during probiotic treatment with medicinal Trichuris suis ova in a healthy volunteer

    DEFF Research Database (Denmark)

    Williams, Andrew R; Dige, Anders; Rasmussen, Tue Kruse

    2017-01-01

    Ingestion of eggs (ova) of the porcine nematode parasite Trichuris suis (TSO) may reduce the severity of autoimmune disorders, however the development of TSO treatment as a useful therapy for autoimmune diseases is hampered by a lack of knowledge on the development of the parasite and the nature...... was also reflected to some extent in the peripheral circulation. These results, together with the first definitive observations that T. suis can mature to adult size and reproduce in humans, shed new light on the interaction between the human immune system and probiotic helminth treatment, which should...

  3. Purification and Characterization of Suicin 65, a Novel Class I Type B Lantibiotic Produced by Streptococcus suis.

    Science.gov (United States)

    Vaillancourt, Katy; LeBel, Geneviève; Frenette, Michel; Fittipaldi, Nahuel; Gottschalk, Marcelo; Grenier, Daniel

    2015-01-01

    Bacteriocins are antimicrobial peptides of bacterial origin that are considered as a promising alternative to the use of conventional antibiotics. Recently, our laboratory reported the purification and characterization of two lantibiotics, suicin 90-1330 and suicin 3908, produced by the swine pathogen and zoonotic agent Streptococcus suis (serotype 2). In this study, a novel bacteriocin produced by S. suis has been identified and characterized. The producing strain S. suis 65 (serotype 2) was found to belong to the sequence type 28, that includes strains known to be weakly or avirulent in a mouse model. The bacteriocin, whose production was only possible following growth on solid culture medium, was purified to homogeneity by cationic exchange and reversed-phase high-pressure liquid chromatography. The bacteriocin, named suicin 65, was heat, pH and protease resistant. Suicin 65 was active against all S. suis isolates tested, including antibiotic resistant strains. Amino acid sequencing of the purified bacteriocin by Edman degradation revealed the presence of modified amino acids suggesting a lantibiotic. Using the partial sequence obtained, a blast was performed against published genomes of S. suis and allowed to identify a putative lantibiotic locus in the genome of S. suis 89-1591. From this genome, primers were designed and the gene cluster involved in the production of suicin 65 by S. suis 65 was amplified by PCR. Sequence analysis revealed the presence of ten open reading frames, including a duplicate of the structural gene. The structural genes (sssA and sssA') of suicin 65 encodes a 25-amino acid residue leader peptide and a 26-amino acid residue mature peptide yielding an active bacteriocin with a deducted molecular mass of 3,005 Da. Mature suicin 65 showed a high degree of identity with class I type B lantibiotics (globular structure) produced by Streptococcus pyogenes (streptococcin FF22; 84.6%), Streptococcus macedonicus (macedocin ACA-DC 198; 84

  4. Purification and Characterization of Suicin 65, a Novel Class I Type B Lantibiotic Produced by Streptococcus suis.

    Directory of Open Access Journals (Sweden)

    Katy Vaillancourt

    Full Text Available Bacteriocins are antimicrobial peptides of bacterial origin that are considered as a promising alternative to the use of conventional antibiotics. Recently, our laboratory reported the purification and characterization of two lantibiotics, suicin 90-1330 and suicin 3908, produced by the swine pathogen and zoonotic agent Streptococcus suis (serotype 2. In this study, a novel bacteriocin produced by S. suis has been identified and characterized. The producing strain S. suis 65 (serotype 2 was found to belong to the sequence type 28, that includes strains known to be weakly or avirulent in a mouse model. The bacteriocin, whose production was only possible following growth on solid culture medium, was purified to homogeneity by cationic exchange and reversed-phase high-pressure liquid chromatography. The bacteriocin, named suicin 65, was heat, pH and protease resistant. Suicin 65 was active against all S. suis isolates tested, including antibiotic resistant strains. Amino acid sequencing of the purified bacteriocin by Edman degradation revealed the presence of modified amino acids suggesting a lantibiotic. Using the partial sequence obtained, a blast was performed against published genomes of S. suis and allowed to identify a putative lantibiotic locus in the genome of S. suis 89-1591. From this genome, primers were designed and the gene cluster involved in the production of suicin 65 by S. suis 65 was amplified by PCR. Sequence analysis revealed the presence of ten open reading frames, including a duplicate of the structural gene. The structural genes (sssA and sssA' of suicin 65 encodes a 25-amino acid residue leader peptide and a 26-amino acid residue mature peptide yielding an active bacteriocin with a deducted molecular mass of 3,005 Da. Mature suicin 65 showed a high degree of identity with class I type B lantibiotics (globular structure produced by Streptococcus pyogenes (streptococcin FF22; 84.6%, Streptococcus macedonicus (macedocin ACA

  5. Streptococcus suis: a re-emerging pathogen associated with occupational exposure to pigs or pork products. Part II – Pathogenesis

    Directory of Open Access Journals (Sweden)

    Jacek Dutkiewicz

    2018-03-01

    Full Text Available Streptococcus suis is a re-emerging zoonotic pathogen that may cause severe disease, mostly meningitis, in pigs and in humans having occupational contact with pigs and pork, such as farmers, slaughterhose workers and butchers. The first stage of the pathogenic process, similar in pigs and humans, is adherence to and colonisation of mucosal and/or epithelial surface(s of the host. The second stage is invasion into deeper tissue and extracellular translocation of bacterium in the bloodstream, either free in circulation or attached to the surface of monocytes. If S. suis present in blood fails to cause fatal septicaemia, it is able to progress into the third stage comprising penetration into host’s organs, mostly by crossing the blood-brain barrier and/or blood–cerebrospinal fluid barrier to gain access to the central nervous system (CNS and cause meningitis. The fourth stage is inflammation that plays a key role in the pathogen esis of both systemic and CNS infections caused by S. suis . The pathogen may induce the overproduction of pro-inflammatory cytokines that cause septic shock and/or the recruitment and activation of different leukocyte populations, causing acute inflammation of the CNS. Streptococcus suis can also evoke – through activation of microglial cells, astrocytes and possibly other cell types – a fulminant inflammatory reaction of the brain which leads to intracranial complications, including brain oedema, increased intracranial pressure, cerebrovascular insults, and deafness, as a result of cochlear sepsis. In all stages of the pathogenic process, S. suis interacts with many types of immunocompetent host’s cells, such as polymorphonuclear leukocytes, mononuclear macrophages, lymphocytes, dendritic cells and microglia, using a range of versatile virulence factors for evasion of the innate and adaptive immune defence of the host, and for overcoming environmental stress. It is estimated that S. suis produces more than

  6. Comparative Genome Analyses of Streptococcus suis Isolates from Endocarditis Demonstrate Persistence of Dual Phenotypic Clones.

    Science.gov (United States)

    Tohya, Mari; Watanabe, Takayasu; Maruyama, Fumito; Arai, Sakura; Ota, Atsushi; Athey, Taryn B T; Fittipaldi, Nahuel; Nakagawa, Ichiro; Sekizaki, Tsutomu

    2016-01-01

    Many bacterial species coexist in the same niche as heterogeneous clones with different phenotypes; however, understanding of infectious diseases by polyphenotypic bacteria is still limited. In the present study, encapsulation in isolates of the porcine pathogen Streptococcus suis from persistent endocarditis lesions was examined. Coexistence of both encapsulated and unencapsulated S. suis isolates was found in 26 out of 59 endocarditis samples. The isolates were serotype 2, and belonged to two different sequence types (STs), ST1 and ST28. The genomes of each of the 26 pairs of encapsulated and unencapsulated isolates from the 26 samples were sequenced. The data showed that each pair of isolates had one or more unique nonsynonymous mutations in the cps gene, and the encapsulated and unencapsulated isolates from the same samples were closest to each other. Pairwise comparisons of the sequences of cps genes in 7 pairs of encapsulated and unencapsulated isolates identified insertion/deletions (indels) ranging from one to 104 bp in different cps genes of unencapsulated isolates. Capsule expression was restored in a subset of unencapsulated isolates by complementation in trans with cps expression vectors. Examination of gene content common to isolates indicated that mutation frequency was higher in ST28 pairs than in ST1 pairs. Genes within mobile genetic elements were mutation hot spots among ST28 isolates. Taken all together, our results demonstrate the coexistence of dual phenotype (encapsulated and unencapsulated) bacterial clones and suggest that the dual phenotypes arose independently in each farm by means of spontaneous mutations in cps genes.

  7. Mechanisms of Host-Pathogen Protein Complex Formation and Bacterial Immune Evasion of Streptococcus suis Protein Fhb.

    Science.gov (United States)

    Li, Xueqin; Liu, Peng; Gan, Shuzhen; Zhang, Chunmao; Zheng, Yuling; Jiang, Yongqiang; Yuan, Yuan

    2016-08-12

    Streptococcus suis serotype 2 (S. suis 2)-induced sepsis and meningitis are often accompanied by bacteremia. The evasion of polymorphonuclear leukocyte-mediated phagocytic clearance is central to the establishment of bacteremia caused by S. suis 2 and is facilitated by the ability of factor H (FH)-binding protein (Fhb) to bind FH on the bacterial surface, thereby impeding alternative pathway complement activation and phagocytic clearance. Here, C3b/C3d was found to bind to Fhb, along with FH, forming a large immune complex. The formation of this immune complex was mediated by domain II of Fhb via electrostatic and hydrophobic interactions, which, to our knowledge, is a new type of interaction. Interestingly, Fhb was found to be associated with the cell envelope and also present in the culture supernatant, where secreted Fhb inhibited complement activation via interactions with domain II, thereby enhancing antiphagocytic clearance by polymorphonuclear leukocytes. Thus, Fhb is a multifunctional bacterial protein, which binds host complement component C3 as well as FH and interferes with innate immune recognition in a secret protein manner. S. suis 2 therefore appears to have developed a new strategy to combat host innate immunity and enhance survival in host blood. © 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

  8. Mechanisms of Host-Pathogen Protein Complex Formation and Bacterial Immune Evasion of Streptococcus suis Protein Fhb*

    Science.gov (United States)

    Li, Xueqin; Liu, Peng; Gan, Shuzhen; Zhang, Chunmao; Zheng, Yuling; Jiang, Yongqiang; Yuan, Yuan

    2016-01-01

    Streptococcus suis serotype 2 (S. suis 2)-induced sepsis and meningitis are often accompanied by bacteremia. The evasion of polymorphonuclear leukocyte-mediated phagocytic clearance is central to the establishment of bacteremia caused by S. suis 2 and is facilitated by the ability of factor H (FH)-binding protein (Fhb) to bind FH on the bacterial surface, thereby impeding alternative pathway complement activation and phagocytic clearance. Here, C3b/C3d was found to bind to Fhb, along with FH, forming a large immune complex. The formation of this immune complex was mediated by domain II of Fhb via electrostatic and hydrophobic interactions, which, to our knowledge, is a new type of interaction. Interestingly, Fhb was found to be associated with the cell envelope and also present in the culture supernatant, where secreted Fhb inhibited complement activation via interactions with domain II, thereby enhancing antiphagocytic clearance by polymorphonuclear leukocytes. Thus, Fhb is a multifunctional bacterial protein, which binds host complement component C3 as well as FH and interferes with innate immune recognition in a secret protein manner. S. suis 2 therefore appears to have developed a new strategy to combat host innate immunity and enhance survival in host blood. PMID:27342778

  9. Use of Antibiotics and Antimicrobial Resistance in Veterinary Medicine as Exemplified by the Swine Pathogen Streptococcus suis.

    Science.gov (United States)

    Seitz, Maren; Valentin-Weigand, Peter; Willenborg, Jörg

    2016-01-01

    Use of antimicrobial agents in veterinary medicine is essential to control infectious diseases, thereby keeping animals healthy and animal products safe for the consumer. On the other hand, development and spread of antimicrobial resistance is of major concern for public health. Streptococcus (S.) suis reflects a typical bacterial pathogen in modern swine production due to its facultative pathogenic nature and wide spread in the pig population. Thus, in the present review we focus on certain current aspects and problems related to antimicrobial use and resistance in S. suis as a paradigm for a bacterial pathogen affecting swine husbandry worldwide. The review includes (i) general aspects of antimicrobial use and resistance in veterinary medicine with emphasis on swine, (ii) genetic resistance mechanisms of S. suis known to contribute to bacterial survival under antibiotic selection pressure, and (iii) possible other factors which may contribute to problems in antimicrobial therapy of S. suis infections, such as bacterial persister cell formation, biofilm production, and co-infections. The latter shows that we hardly understand the complexity of factors affecting the success of antimicrobial treatment of (porcine) infectious diseases and underlines the need for further research in this field.

  10. Detection of a quantitative trait locus associated with resistance to infection with Trichuris suis in pigs

    DEFF Research Database (Denmark)

    Skallerup, Per; Thamsborg, Stig Milan; Jørgensen, Claus Bøttcher

    2015-01-01

    diarrhoea. A significant proportion of the variation in Trichuris faecal egg count (FEC) has been attributed to the host's genetic make-up. The aim of the present study was to identify genetic loci associated with resistance to T. suis in pigs. We used single nucleotide polymorphism (SNP) markers to perform...

  11. The first report on Cryptosporidium suis and Cryptosporidium pig genotype II in Eurasian wild boars (Sus scrofa) (Czech Republic)

    Czech Academy of Sciences Publication Activity Database

    Němejc, K.; Sak, Bohumil; Květoňová, Dana; Hanzal, V.; Jeníková, Martina; Kváč, Martin

    2012-01-01

    Roč. 184, 2/4 (2012), 122-125 ISSN 0304-4017 Grant - others:Mšk(CZ) 6007665806 Institutional research plan: CEZ:AV0Z60220518; CEZ:AV0Z50450515 Keywords : Cryptosporidium suis * Cryptosporidium pig genotype II * Eurasian wild boar * SSU * PCR Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.381, year: 2012

  12. Changes in abundance of Lactobacillus spp. and Streptococcus suis in the stomach, jejunum and ileum of piglets after weaning

    NARCIS (Netherlands)

    Su, Y.; Yao, W.; Perez-Gutierrez, O.N.; Smidt, H.; Zhu, W.Y.

    2008-01-01

    This present study investigated the changes in bacterial community composition, with an emphasis on Lactobacillus spp. and Streptococcus suis populations as potentially beneficial and harmful groups, in the stomach, jejunum and ileum of piglets after weaning (21 days postpartum) by 16S rRNA

  13. Characterization of virulence of the Streptococcus suis serotype 2 reference strain Henrichsen S 735 in newborn gnotobiotic pigs

    NARCIS (Netherlands)

    Vecht, U.; Wisselink, H.J.; Stockhofe-Zurwieden, N.; Smith, H.E.

    1996-01-01

    Strain Henrichsen S 735 (NCTC 10234) of Streptococcus suis serotype 2 reference and three other such strains (strains S 4005, S 3921 and T 141) were tested for virulence by inoculating pigs intranasally and intravenously. The taxonomical properties of each strain were determined. Phenotypes were

  14. Correlation between PFGE Groups and mrp/epf/sly Genotypes of Human Streptococcus suis Serotype 2 in Northern Thailand

    Directory of Open Access Journals (Sweden)

    Prasit Tharavichitkul

    2014-01-01

    Full Text Available Streptococcus suis infection is a severe zoonotic disease commonly found in Northern Thailand where people often consume raw pork and/or pig’s blood. The most frequent clinical presentations are meningitis, sepsis, and endocarditis with higher rate of mortality and hearing loss sequelae. To clarify the correlation between pulsed-field gel electrophoresis (PFGE groups and mrp/epf/sly genotypes of S. suis serotype 2, 62 patient and 4 healthy pig isolates from Northern Thailand were studied. By PFGE analysis, at 66% homology, most human isolates (69.4% and 1 pig isolate were in group A, whereas 14.5% of human isolates and 3 out of 4 pig isolates were in group D. According to mrp/epf/sly genotypes, 80.6% of human isolates were identified in mrp+epf−sly− and only 12.9% were in mrp−epf−sly+ genotypes; in contrast, 1 and 3 pig isolates were detected in these two genotypes, respectively. Interestingly, all isolates of S. suis serotype 2 classified in PFGE groups A, B, and E were set in mrp+epf−sly− genotypes. These data show a close correlation between PFGE groups and mrp/epf/sly genotypes of human S. suis serotype 2.

  15. Correlation between PFGE Groups and mrp/epf/sly Genotypes of Human Streptococcus suis Serotype 2 in Northern Thailand.

    Science.gov (United States)

    Tharavichitkul, Prasit; Wongsawan, Kanreuthai; Takenami, Naoki; Pruksakorn, Sumalee; Fongcom, Achara; Gottschalk, Marcelo; Khanthawa, Banyong; Supajatura, Volaluk; Takai, Shinji

    2014-01-01

    Streptococcus suis infection is a severe zoonotic disease commonly found in Northern Thailand where people often consume raw pork and/or pig's blood. The most frequent clinical presentations are meningitis, sepsis, and endocarditis with higher rate of mortality and hearing loss sequelae. To clarify the correlation between pulsed-field gel electrophoresis (PFGE) groups and mrp/epf/sly genotypes of S. suis serotype 2, 62 patient and 4 healthy pig isolates from Northern Thailand were studied. By PFGE analysis, at 66% homology, most human isolates (69.4%) and 1 pig isolate were in group A, whereas 14.5% of human isolates and 3 out of 4 pig isolates were in group D. According to mrp/epf/sly genotypes, 80.6% of human isolates were identified in mrp (+) epf (-) sly (-) and only 12.9% were in mrp (-) epf (-) sly (+) genotypes; in contrast, 1 and 3 pig isolates were detected in these two genotypes, respectively. Interestingly, all isolates of S. suis serotype 2 classified in PFGE groups A, B, and E were set in mrp (+) epf (-) sly (-) genotypes. These data show a close correlation between PFGE groups and mrp/epf/sly genotypes of human S. suis serotype 2.

  16. Une étude de cas. Saint Sébastien dans “Je suis sang” de Jan Fabre

    Directory of Open Access Journals (Sweden)

    Lydie Toran

    2017-06-01

    Full Text Available Jan Fabre’s writing frequently displays a variety of quotations, especially visual ones. His 2001 Je suis sang (conte de fées médiéval is directly and indirectly inspired by Saint Sebastian’s iconography. This study examines the modalities and functions of this re-use in detail.

  17. Antimicrobial Resistance Profile and Genotypic Characteristics of Streptococcus suis Capsular Type 2 Isolated from Clinical Carrier Sows and Diseased Pigs in China

    Directory of Open Access Journals (Sweden)

    Chunping Zhang

    2015-01-01

    Full Text Available Streptococcus suis serotype 2 is an important zoonotic pathogen. Antimicrobial resistance phenotypes and genotypic characterizations of S. suis 2 from carrier sows and diseased pigs remain largely unknown. In this study, 96 swine S. suis type 2, 62 from healthy sows and 34 from diseased pigs, were analyzed. High frequency of tetracycline resistance was observed, followed by sulfonamides. The lowest resistance of S. suis 2 for β-lactams supports their use as the primary antibiotics to treat the infection of serotype 2. In contrast, 35 of 37 S. suis 2 with MLSB phenotypes were isolated from healthy sows, mostly encoded by the ermB and/or the mefA genes. Significantly lower frequency of mrp+/epf+/sly+ was observed among serotype 2 from healthy sows compared to those from diseased pigs. Furthermore, isolates from diseased pigs showed more homogeneously genetic patterns, with most of them clustered in pulsotypes A and E. The data indicate the genetic complexity of S. suis 2 between herds and a close linkage among isolates from healthy sows and diseased pigs. Moreover, many factors, such as extensive use of tetracycline or diffusion of Tn916 with tetM, might have favored for the pathogenicity and widespread dissemination of S. suis serotype 2.

  18. Characterization of the Pivotal Carbon Metabolism of Streptococcus suis Serotype 2 under ex Vivo and Chemically Defined in Vitro Conditions by Isotopologue Profiling*

    Science.gov (United States)

    Willenborg, Jörg; Huber, Claudia; Koczula, Anna; Lange, Birgit; Eisenreich, Wolfgang; Valentin-Weigand, Peter; Goethe, Ralph

    2015-01-01

    Streptococcus suis is a neglected zoonotic pathogen that has to adapt to the nutritional requirements in the different host niches encountered during infection and establishment of invasive diseases. To dissect the central metabolic activity of S. suis under different conditions of nutrient availability, we performed labeling experiments starting from [13C]glucose specimens and analyzed the resulting isotopologue patterns in amino acids of S. suis grown under in vitro and ex vivo conditions. In combination with classical growth experiments, we found that S. suis is auxotrophic for Arg, Gln/Glu, His, Leu, and Trp in chemically defined medium. De novo biosynthesis was shown for Ala, Asp, Ser, and Thr at high rates and for Gly, Lys, Phe, Tyr, and Val at moderate or low rates, respectively. Glucose degradation occurred mainly by glycolysis and to a minor extent by the pentose phosphate pathway. Furthermore, the exclusive formation of oxaloacetate by phosphoenolpyruvate (PEP) carboxylation became evident from the patterns in de novo synthesized amino acids. Labeling experiments with S. suis grown ex vivo in blood or cerebrospinal fluid reflected the metabolic adaptation to these host niches with different nutrient availability; however, similar key metabolic activities were identified under these conditions. This points at the robustness of the core metabolic pathways in S. suis during the infection process. The crucial role of PEP carboxylation for growth of S. suis in the host was supported by experiments with a PEP carboxylase-deficient mutant strain in blood and cerebrospinal fluid. PMID:25575595

  19. A repA-based ELISA for discriminating cattle vaccinated with Brucella suis 2 from those naturally infected with Brucella abortus and Brucella melitensis.

    Science.gov (United States)

    Wang, Jing-Yu; Wu, Ning; Liu, Wan-Hua; Ren, Juan-Juan; Tang, Pan; Qiu, Yuan-Hao; Wang, Chi-Young; Chang, Ching-Dong; Liu, Hung-Jen

    2014-01-01

    The commonest ways of diagnosing brucellosis in animals include the Rose-Bengal plate agglutination test, the buffered plate agglutination test (BPA), the slide agglutination test, the complement fixation test, and the indirect enzyme linked immunosorbent assay (I-ELISA). However, these methods cannot discriminate the Brucella vaccine strain (Brucella suis strain 2; B. suis S2) from naturally acquired virulent strains. Of the six common Brucella species, Brucella melitensis, Brucella abortus, and B. suis are the commonest species occurring in China. To develop an ELISA assay that can differentiate between cows inoculated with B. suis S2 and naturally infected with B. abortus and B. melitensis, genomic sequences from six Brucella spp. (B. melitensis, B. abortus, B. suis, Brucella canis, Brucella neotomae and Brucella ovis) were compared using Basic Local Alignment Search Tool software. One particular gene, the repA-related gene, was found to be a marker that can differentiate B. suis from B. abortus and B. melitensis. The repA-related gene of B. suis was PCR amplified and subcloned into the pET-32a vector. Expressed repA-related protein was purified and used as an antigen. The repA-based ELISA was optimized and used as specific tests. In the present study, serum from animals inoculated with the B. suis S2 vaccine strain had positive repA-based ELISA results. In contrast, the test-positive reference sera against B. abortus and B. melitensis had negative repA-based ELISA results. The concordance rate between B. abortus antibody-negative (based on the repA-based ELISA) and the Brucella gene-positive (based on the 'Bruce ladder' multiplex PCR) was 100%. Therefore, the findings suggest that the repA-based ELISA is a useful tool for differentiating cows vaccinated with the B. suis S2 and naturally infected with B. abortus and B. melitensis. Copyright © 2014 Elsevier Ltd. All rights reserved.

  20. ISOLATION AND IDENTIFICATION OF BRUCELLA SUIS IN PIGS AS ZOONOTIC DISEASE IN ENDEMIC AREAS OF EAST JAVA, INDONESIA.

    Science.gov (United States)

    S, Emy Koestanti; Misaco, Wiwik; Chusniati, Sri; Maslachah, Lilik

    2018-01-01

    Brucellosis in pigs at East Java Indonesia has not only cause great economic losses due to a decrease in productivity of livestock but also are zoonotic. Infection on free brucelosis pigs were initially begun with the infected pigs both male and female, or the use of superior male pigs together. The elimination of the disease either on a group or population is considered as the most effective way to prevent the spread of the disease in pigs. Prevention efforts mainly addressed to vaccination, sanitary maintenace and government policy. The purpose of this study was to isolated and identified Brucella suis as the causative agent. The survey area were the pig farm owned by breeder farmers in the area of East Java Indonesia, at Kediri, Malang, Blitar and Probolinggo district. Blood samples obtained were tested with RBT. Pigs are suspected of being infected with Brucella if the RBT was positive that characterized with agglutination in the test results. If RBT was positive, bacteriological examination will be performed, with samples of visceral foetus organ, ie liver, spleen, placenta and amniotic fluid. Isolation and identification of Brucella suis were used Brucella Broth and Brucella Agar, and if the bacteri growthwill be continued with biochemical test ie H2S, urease, citrate, catalase and oxidase test. The positive results of Brucella suis showed positive urease, catalase andoxidase, but negative for citrate and H2S. RBT and bacteriolgical examination showed that 1 sample was positive Brucella suis , and 19 negative. The positive results showed positive urease, catalase and oxidase, but negative for citrate and H2S. Based on RBT test and bacteriological examination, there was 1 positive sample of brucellla suis, that is sample coming from Kediri district.

  1. "Un alto en el camino para saber cuántos somos…": Los censos de población y la construcción de lealtades nacionales. Chile, siglo XIX

    Directory of Open Access Journals (Sweden)

    Andrés Estefane Jaramillo

    2004-06-01

    Full Text Available El presente trabajo pretende interpretar los censos de población aplicados en Chile durante el siglo XIX como eventos de carácter nacional que habrían contribuido al complejo proceso de construcción social de la nación. Para ello fija su atención en la institución que los organiza, el Estado, que se ve en la obligación de aplicar mecanismos cooptativos y coactivos orientados a eliminar la resistencia de la población ante este tipo de empadronamientos y de esta forma asegurar la correcta aplicación de la encuesta. Asimismo, analiza que papel que tuvieron las autoridades locales en estos eventos, toda vez que la materialización de la iniciativa dependía de la voluntad de aquellos actores influyentes en los espacios básicos de organización social. Por otra parte, estudia la dimensión territorial que se desprende de la aplicación del censo, como también los intentos de la ciencia estadística por homogeneizar una realidad social heterogénea. Por último, ofrece algunas vías interpretativas que demuestran la riqueza de esta fuente para comprender aspectos poco estudiados de nuestra historia nacional.The current paper attempts to interpret the censuses of the population carried out in Chile during the XIX century as events of a national character that would have contributed to the complex process of social construction of the nation. For doing that it focuses its attention on the institution that organizes them, the State, which is forced to apply cooperative and coactive mechanisms orientated to eliminate the population's resistance to this kind of registration and thus guaranteeing the correct application of the survey. Likewise, it analyses the role the local authorities played in these events, considering that the implementation of the initiative depended on the will of those influential actors in the basic spaces of the social organization. On the other hand, it studies the territorial dimension that one gathers from the

  2. Adhesion activity of glyceraldehyde-3-phosphate dehydrogenase in a Chinese Streptococcus suis type 2 strain.

    Science.gov (United States)

    Wang, Kaicheng; Lu, Chengping

    2007-01-01

    A total of 36 streptococcal strains, including seven S. equi ssp.zooepidemicus, two S. suis type 1 (SS1), 24 SS2, two SS9, and one SS7, were tested for glyceraldehyde-3-phosphate dehydrogenase gene (gapdh). Except from non-virulent SS2 strain T1 5, all strains harboured gapdh. The gapdh of Chinese Sichuan SS2 isolate ZY05719 and Jiangsu SS2 isolate HA9801 were sequenced and then compared with published sequences in the GenBank. The comparison revealed a 99.9 % and 99.8 % similarity of ZY05719 and HA9801, respectively, with the published sequence. Adherence assay data demonstrated a significant ((p<0.05)) reduction in adhesion of SS2 in HEp-2 cells pre-incubated with purified GAPDH compared to non pre-incubated controls, suggesting the GAPDH mediates SS2 bacterial adhesion to host cells.

  3. Identification and characterization of a novel protective antigen, Enolase of Streptococcus suis serotype 2.

    Science.gov (United States)

    Zhang, Anding; Chen, Bo; Mu, Xiaofeng; Li, Ran; Zheng, Pei; Zhao, Yaxin; Chen, Huanchun; Jin, Meilin

    2009-02-25

    Streptococcus suis serotype 2 (SS2) is a porcine and human pathogen with adhesive and invasive properties. The absence of suitable vaccine or virulent marker can be the bottleneck to control SS2 infection. In the present study, a novel immunogenic Enolase identified in the previous study was inducibly overexpressed in Escherichia coli, and the purified recombinant protein could elicit a significant humoral antibody response and confer efficient immunity against challenge with lethal dose of SS2 or SS7 infection in mouse model. The roles Enolase plays in pathogenicity of SS2 were also explored as reasons for which Enolase could be a protective antigen. The Enolase was an in vivo-induced antigen confirmed by the real-time PCR and could adhere to the Hep-2 cells by the indirect immunofluorescent assay and the inhibition assay. These suggested that Enolase could play important roles in pathogenicity and may serve as a novel vaccine candidate against SS2 infection.

  4. Molecular Basis of Resistance to Selected Antimicrobial Agents in the Emerging Zoonotic Pathogen Streptococcus suis.

    Science.gov (United States)

    Gurung, Mamata; Tamang, Migma Dorji; Moon, Dong Chan; Kim, Su-Ran; Jeong, Jin-Ha; Jang, Geum-Chan; Jung, Suk-Chan; Park, Yong-Ho; Lim, Suk-Kyung

    2015-07-01

    Characterization of 227 Streptococcus suis strains isolated from pigs during 2010 to 2013 showed high levels of resistance to clindamycin (95.6%), tilmicosin (94.7%), tylosin (93.8%), oxytetracycline (89.4%), chlortetracycline (86.8%), tiamulin (72.7%), neomycin (70.0%), enrofloxacin (56.4%), penicillin (56.4%), ceftiofur (55.9%), and gentamicin (55.1%). Resistance to tetracyclines, macrolides, aminoglycosides, and fluoroquinolone was attributed to the tet gene, erm(B), erm(C), mph(C), and mef(A) and/or mef(E) genes, aph(3')-IIIa and aac(6')-Ie-aph(2″)-Ia genes, and single point mutations in the quinolone resistance-determining region of ParC and GyrA, respectively. Copyright © 2015, American Society for Microbiology. All Rights Reserved.

  5. Crystallization and preliminary crystallographic analysis of recombinant immunoglobulin G-binding protein from Streptococcus suis

    Energy Technology Data Exchange (ETDEWEB)

    Khan, Abdul Hamid; Chu, Fuliang; Feng, Youjun; Zhang, Qinagmin [Center for Molecular Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101 (China); Qi, Jianxun [Institute of Physics, Chinese Academy of Sciences, Beijing 100080 (China); Gao, George Fu, E-mail: gaof@im.ac.cn [Center for Molecular Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101 (China)

    2008-08-01

    Crystallization of recombinant IgG-binding protein expressed in Escherichia coli using the hanging-drop vapour-diffusion method is described. The crystals belonged to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 38.98, b = 43.94, c = 78.17 Å. Streptococcus suis, an important zoonotic pathogen, expresses immunoglobulin G-binding protein, which is thought to be helpful to the organism in eluding the host defence system. Recombinant IgG-binding protein expressed in Escherichia coli has been crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group P2{sub 1}2{sub 1}2{sub 1}, with unit-cell parameters a = 38.98, b = 43.94, c = 78.17 Å and one molecule in the asymmetric unit. Diffraction data were collected to 2.60 Å resolution.

  6. Comparative Genome Analyses of Streptococcus suis Isolates from Endocarditis Demonstrate Persistence of Dual Phenotypic Clones.

    Directory of Open Access Journals (Sweden)

    Mari Tohya

    Full Text Available Many bacterial species coexist in the same niche as heterogeneous clones with different phenotypes; however, understanding of infectious diseases by polyphenotypic bacteria is still limited. In the present study, encapsulation in isolates of the porcine pathogen Streptococcus suis from persistent endocarditis lesions was examined. Coexistence of both encapsulated and unencapsulated S. suis isolates was found in 26 out of 59 endocarditis samples. The isolates were serotype 2, and belonged to two different sequence types (STs, ST1 and ST28. The genomes of each of the 26 pairs of encapsulated and unencapsulated isolates from the 26 samples were sequenced. The data showed that each pair of isolates had one or more unique nonsynonymous mutations in the cps gene, and the encapsulated and unencapsulated isolates from the same samples were closest to each other. Pairwise comparisons of the sequences of cps genes in 7 pairs of encapsulated and unencapsulated isolates identified insertion/deletions (indels ranging from one to 104 bp in different cps genes of unencapsulated isolates. Capsule expression was restored in a subset of unencapsulated isolates by complementation in trans with cps expression vectors. Examination of gene content common to isolates indicated that mutation frequency was higher in ST28 pairs than in ST1 pairs. Genes within mobile genetic elements were mutation hot spots among ST28 isolates. Taken all together, our results demonstrate the coexistence of dual phenotype (encapsulated and unencapsulated bacterial clones and suggest that the dual phenotypes arose independently in each farm by means of spontaneous mutations in cps genes.

  7. Comparative Genome Analyses of Streptococcus suis Isolates from Endocarditis Demonstrate Persistence of Dual Phenotypic Clones

    Science.gov (United States)

    Tohya, Mari; Watanabe, Takayasu; Maruyama, Fumito; Arai, Sakura; Ota, Atsushi; Athey, Taryn B. T.; Fittipaldi, Nahuel; Nakagawa, Ichiro; Sekizaki, Tsutomu

    2016-01-01

    Many bacterial species coexist in the same niche as heterogeneous clones with different phenotypes; however, understanding of infectious diseases by polyphenotypic bacteria is still limited. In the present study, encapsulation in isolates of the porcine pathogen Streptococcus suis from persistent endocarditis lesions was examined. Coexistence of both encapsulated and unencapsulated S. suis isolates was found in 26 out of 59 endocarditis samples. The isolates were serotype 2, and belonged to two different sequence types (STs), ST1 and ST28. The genomes of each of the 26 pairs of encapsulated and unencapsulated isolates from the 26 samples were sequenced. The data showed that each pair of isolates had one or more unique nonsynonymous mutations in the cps gene, and the encapsulated and unencapsulated isolates from the same samples were closest to each other. Pairwise comparisons of the sequences of cps genes in 7 pairs of encapsulated and unencapsulated isolates identified insertion/deletions (indels) ranging from one to 104 bp in different cps genes of unencapsulated isolates. Capsule expression was restored in a subset of unencapsulated isolates by complementation in trans with cps expression vectors. Examination of gene content common to isolates indicated that mutation frequency was higher in ST28 pairs than in ST1 pairs. Genes within mobile genetic elements were mutation hot spots among ST28 isolates. Taken all together, our results demonstrate the coexistence of dual phenotype (encapsulated and unencapsulated) bacterial clones and suggest that the dual phenotypes arose independently in each farm by means of spontaneous mutations in cps genes. PMID:27433935

  8. Elementos para la construcción de una política pública de bilingüismo en el Valle del Cauca: un análisis descriptivo a partir del censo ampliado de 2005

    Directory of Open Access Journals (Sweden)

    Julio César Alonso

    2012-01-01

    Full Text Available Los índices de competitividad reflejan un pobre desempeño del departamento del Valle del Cauca, especialmente en lo referente al capital humano. Una política pública de bilingüismo permite acumular capital humano a través de la educación en inglés, acceder a nuevos mercados y mejor información, lo que hace posible el desarrollo de otros factores necesarios para la competitividad de una región. En este documento se hace un primer diagnóstico del bilingüismo en el Valle del Cauca, con miras a brindar argumentos para la creación de una eficaz política pública de bilingüismo. Los datos procesados provienen del Censo ampliado de 2005, y los resultados obtenidos a partir de él no son alentadores, pues muestran la necesidad de convertir el bilingüismo en un tema de prioridad en la agenda pública.

  9. La mujer mexicana económicamente activa: ¿son confiables los microdatos censales? Una prueba a través de censos y encuestas. México y Estados Unidos, 1970-1990

    Directory of Open Access Journals (Sweden)

    Gabriela Vázquez

    2000-01-01

    Full Text Available La propuesta temática de este artículo se sustenta en el desarrollo del proyecto Microdatos Seriados e Integrados de Uso Público Internacional (IPUMS, que inició en octubre de 1999, auspiciado por el Minnesota Population Center (MPC de la Universidad de Minnesota. Intentamos probar la utilidad de una base de datos como la que propone crear IPUMS. Así, a pesar de que no se cuenta aún con datos integrados, procuraremos mostrar la potencialidad de la fuente de datos que agrupe información censal de diferentes países. Dentro de una perspectiva comparativa, hemos desarrollado un análisis multivariado a través de la comparación entre censos y la Encuesta Nacional de Empleo Urbano (ENEU, tanto en el tiempo (1970 y 1990 como entre países (México y Estados Unidos, refiriéndonos a la mujer mexicana, en particular a partir del estudio de la fuerza de trabajo femenina.

  10. Awakening – Transformation, agency and virtue from three ...

    African Journals Online (AJOL)

    structure' dualism and suspension of ontology in body-mind dualism and ... In the complexity of possible relations between self, self-concept and society, lies the traditionally neglected transformative middle of sui-generis human depth.

  11. to view fulltext PDF

    Indian Academy of Sciences (India)

    Unknown

    1977-03-02

    Mar 2, 1977 ... 431. Keywords. Access; rural communities; sui generis legislation; traditional knowledge ..... According to the village elder, perhaps this modern health center had ...... her Education, Scientific and Technical Research, Trade.

  12. Targeting TREM-1 signaling in the presence of antibiotics is effective against Streptococcal toxic-shock-like syndrome (STSLS caused by Streptococcus suis

    Directory of Open Access Journals (Sweden)

    Chao eYang

    2015-11-01

    Full Text Available Streptococcus suis (S. suis, a major swine pathogen, is also a severe threat to human health. Infection with highly virulent strains of S. suis can cause human Streptococcal toxic-shock-like syndrome (STSLS, which is associated with high serum pro-inflammatory cytokine levels and a high mortality rate. Our previous study indicated that highly virulent S. suis infection could activate the TREM-1 signaling pathway, which promotes host clearance of S. suis during early infection. However, it remained to be elicited whether TREM-1 signaling could be a target against STSLS in the presence of antibiotic. In the present study, mice were infected with a highly virulent S. suis strain and then treated with rTREM-1 (the recombinant extracellular domain of TREM-1 to block TREM-1 signaling, antibiotics, both rTREM-1 and antibiotics, or PBS. The survival rates, clinical signs, serum IL-1β and TNF-α levels, and serum bacterial loads were evaluated. Treatment with rTREM-1 could aggravate the outcome of infection as described previously. Although the conventional treatment with antibiotics contributed to effective S. suis clearance, it did not improve survival significantly. In comparison, due to the reduction of the exaggerated pro-inflammatory response, treatment combined with rTREM-1 and antibiotics not only led to efficient bacterial clearance but also alleviated inflammation. In conclusion, TREM-1 signaling contributed to severe inflammatory response and benefited S. suis clearance. Therefore, blocking TREM-1 signaling could still be a target for the treatment of STSLS in the presence of antibiotics.

  13. Binding of Human Fibrinogen to MRP Enhances Streptococcus suis Survival in Host Blood in a αXβ2 Integrin-dependent Manner.

    Science.gov (United States)

    Pian, Yaya; Li, Xueqin; Zheng, Yuling; Wu, Xiaohong; Yuan, Yuan; Jiang, Yongqiang

    2016-05-27

    The Gram-positive bacterium Streptococcus suis serotype 2 (S. suis 2), an important zoonotic pathogen, induces strong systemic infections in humans; sepsis and meningitis are the most common clinical manifestations and are often accompanied by bacteremia. However, the mechanisms of S. suis 2 survival in human blood are not well understood. In our previous study, we identified muramidase-released protein (MRP), a novel human fibrinogen (hFg)-binding protein (FBP) in S. suis 2 that is an important epidemic infection marker with an unknown mechanism in pathogenesis. The present study demonstrates that the N-terminus of MRP (a.a. 283-721) binds to both the Aα and Bβ chains of the D fragment of hFg. Strikingly, the hFg-MRP interaction improved the survival of S. suis 2 in human blood and led to the aggregation and exhaustion of polymorphonuclear neutrophils (PMNs) via an αXβ2 integrin-dependent mechanism. Other Fg-binding proteins, such as M1 (GAS) and FOG (GGS), also induced PMNs aggregation; however, the mechanisms of these FBP-hFg complexes in the evasion of PMN-mediated innate immunity remain unclear. MRP is conserved across highly virulent strains in Europe and Asia, and these data shed new light on the function of MRP in S. suis pathogenesis.

  14. FlpS, the FNR-Like Protein of Streptococcus suis Is an Essential, Oxygen-Sensing Activator of the Arginine Deiminase System

    Directory of Open Access Journals (Sweden)

    Jörg Willenborg

    2016-07-01

    Full Text Available Streptococcus (S. suis is a zoonotic pathogen causing septicemia and meningitis in pigs and humans. During infection S. suis must metabolically adapt to extremely diverse environments of the host. CcpA and the FNR family of bacterial transcriptional regulators are important for metabolic gene regulation in various bacteria. The role of CcpA in S. suis is well defined, but the function of the FNR-like protein of S. suis, FlpS, is yet unknown. Transcriptome analyses of wild-type S. suis and a flpS mutant strain suggested that FlpS is involved in the regulation of the central carbon, arginine degradation and nucleotide metabolism. However, isotopologue profiling revealed no substantial changes in the core carbon and amino acid de novo biosynthesis. FlpS was essential for the induction of the arcABC operon of the arginine degrading pathway under aerobic and anaerobic conditions. The arcABC-inducing activity of FlpS could be associated with the level of free oxygen in the culture medium. FlpS was necessary for arcABC-dependent intracellular bacterial survival but redundant in a mice infection model. Based on these results, we propose that the core function of S. suis FlpS is the oxygen-dependent activation of the arginine deiminase system.

  15. Streptococcus suis, an important pig pathogen and emerging zoonotic agent—an update on the worldwide distribution based on serotyping and sequence typing

    Science.gov (United States)

    Goyette-Desjardins, Guillaume; Auger, Jean-Philippe; Xu, Jianguo; Segura, Mariela; Gottschalk, Marcelo

    2014-01-01

    Streptococcus suis is an important pathogen causing economic problems in the pig industry. Moreover, it is a zoonotic agent causing severe infections to people in close contact with infected pigs or pork-derived products. Although considered sporadic in the past, human S. suis infections have been reported during the last 45 years, with two large outbreaks recorded in China. In fact, the number of reported human cases has significantly increased in recent years. In this review, we present the worldwide distribution of serotypes and sequence types (STs), as determined by multilocus sequence typing, for pigs (between 2002 and 2013) and humans (between 1968 and 2013). The methods employed for S. suis identification and typing, the current epidemiological knowledge regarding serotypes and STs and the zoonotic potential of S. suis are discussed. Increased awareness of S. suis in both human and veterinary diagnostic laboratories and further establishment of typing methods will contribute to our knowledge of this pathogen, especially in regions where complete and/or recent data is lacking. More research is required to understand differences in virulence that occur among S. suis strains and if these differences can be associated with specific serotypes or STs. PMID:26038745

  16. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of SAICAR synthase from Streptococcus suis serotype 2

    International Nuclear Information System (INIS)

    Cheng, Xia; Lu, Guangwen; Qi, Jianxun; Cheng, Hao; Gao, Feng; Wang, Jundong; Yan, Jinghua

    2010-01-01

    Crystals of SAICAR synthase from S. suis serotype 2 were obtained in the presence of 40 mM aspartic acid substrate; they belonged to space group P2 and diffracted to 2.8 Å resolution. Phosphoribosylaminoimidazole-succinocarboxamide synthase (SAICAR synthase) plays an essential role in the de novo biosynthesis of purine nucleotides. In this study, the SAICAR synthase from Streptococcus suis was cloned and overexpressed in Escherichia coli. The subsequent product was purified and crystallized using the hanging-drop vapour-diffusion method. The crystals diffracted to 2.8 Å resolution and belonged to space group P2, with unit-cell parameters a = 70.2, b = 52.2, c = 153.9 Å, β = 102.8°

  17. MsmK, an ATPase, Contributes to Utilization of Multiple Carbohydrates and Host Colonization of Streptococcus suis.

    Science.gov (United States)

    Tan, Mei-Fang; Gao, Ting; Liu, Wan-Quan; Zhang, Chun-Yan; Yang, Xi; Zhu, Jia-Wen; Teng, Mu-Ye; Li, Lu; Zhou, Rui

    2015-01-01

    Acquisition and metabolism of carbohydrates are essential for host colonization and pathogenesis of bacterial pathogens. Different bacteria can uptake different lines of carbohydrates via ABC transporters, in which ATPase subunits energize the transport though ATP hydrolysis. Some ABC transporters possess their own ATPases, while some share a common ATPase. Here we identified MsmK, an ATPase from Streptococcus suis, an emerging zoonotic bacterium causing dead infections in pigs and humans. Genetic and biochemistry studies revealed that the MsmK was responsible for the utilization of raffinose, melibiose, maltotetraose, glycogen and maltotriose. In infected mice, the msmK-deletion mutant showed significant defects of survival and colonization when compared with its parental and complementary strains. Taken together, MsmK is an ATPase that contributes to multiple carbohydrates utilization and host colonization of S. suis. This study gives new insight into our understanding of the carbohydrates utilization and its relationship to the pathogenesis of this zoonotic pathogen.

  18. Streptococcus suis: a re-emerging pathogen associated with occupational exposure to pigs or pork products. Part I - Epidemiology.

    Science.gov (United States)

    Dutkiewicz, Jacek; Sroka, Jacek; Zając, Violetta; Wasiński, Bernard; Cisak, Ewa; Sawczyn, Anna; Kloc, Anna; Wójcik-Fatla, Angelina

    2017-12-23

    Streptococcus suis (ex Elliot 1966, Kilpper-Bälz & Schleifer 1987) is a facultatively anaerobic Gram-positive ovoid or coccal bacterium surrounded by a polysaccharide capsule. Based on the antigenic diversity of the capsule, S. suis strains are classified serologically into 35 serotypes. Streptococcus suis is a commensal of pigs, commonly colonizing their tonsils and nasal cavities, mostly in weaning piglets between 4-10 weeks of age. This species occurs also in cattle and other mammals, in birds and in humans. Some strains, mostly those belonging to serotype 2, are also pathogenic for pigs, as well as for other animals and humans. Meningitis is the primary disease syndrome caused by S. suis, both in pigs and in humans. It is estimated that meningitis accounted for 68.0% of all cases of human disease reported until the end of 2012, followed by septicaemia (including life-threatening condition described as 'streptococcal toxic shock-like syndrome' - STSLS), arthritis, endocarditis, and endophthalmitis. Hearing loss and/or ves tibular dysfunction are the most common sequelae after recovery from meningitis caused by S. suis, occurring in more than 50% of patients. In the last two decades, the number of reported human cases due to S. suis has dramatically increased, mostly due to epidemics recorded in China in 1998 and 2005, and the fulminant increase in morbidity in the countries of south-eastern Asia, mostly Vietnam and Thailand. Out of 1,642 cases of S. suis infections identified between 2002-2013 worldwide in humans, 90.2% occurred in Asia, 8.5% in Europe and 1.3% in other parts of the globe. The human disease has mostly a zoonotic and occupational origin and occurs in pig breeders, abattoir workers, butchers and workers of meat processing facilities, veterinarians and meat inspectors. Bacteria are transmitted to workers by close contact with pigs or pig products, usually through contamination of minor cuts or abrasions on skin of hands and/or arms, or by pig bite

  19. Epidemiological investigation of the first human brucellosis case in Spain due to Brucella suis biovar 1 strain 1330.

    Science.gov (United States)

    Compés Dea, Cecilia; Guimbao Bescós, Joaquín; Alonso Pérez de Ágreda, Juan Pablo; Muñoz Álvaro, Pilar María; Blasco Martínez, José María; Villuendas Usón, María Cruz

    2017-03-01

    No cases of human brucellosis caused by Brucella suis has been reported in Spain. This study involved interviews with the case and his co-workers, inspection of their workplace, checking infection control measures, and typing the Brucella strain isolated in the blood culture. Brucella suis biovar 1 strain 1330 was isolated from a patient who worked in a waste treatment plant. Food borne transmission, contact with animals, and risk jobs were ruled out. An accidental inoculation with a contaminated needle from a research laboratory waste container was identified as the most probable mode of transmission. There should be controls to ensure that waste containers are sealed. Copyright © 2016 Elsevier España, S.L.U. and Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica. All rights reserved.

  20. Cell-mediated immune responses differentiate infections with Brucella suis from Yersinia enterocolitica serotype O : 9 in pigs

    DEFF Research Database (Denmark)

    Riber, Ulla; Jungersen, Gregers

    2007-01-01

    Due to almost identical lipopolysaccharide (LPS) O-antigens, infections with Yersinia enterocolitica serotype 0:9 (YeO:9) cause false positive serological reactions (FPSR) in tests for Brucella and thus cause problems in National Brucella surveillance programs. As LPS are strong inducers...... of antibody responses it was hypothesized that cell-mediated immune responses to non-LPS antigens of the two bacteria can be used to separate immune responses to these two biologically very different infections. Following subclinical experimental infections with Brucella suis biovar 2, high interferon......-gamma (IFN-gamma) assay responses with a commercial Brucella melitensis antigen preparation (Brucellergene OCB) preceded the development of antibodies. High IFN-gamma responses in the seven B. suis inoculated pigs with serological evidence of infection were consistent throughout a 20-week postinoculation...

  1. Streptococcus suis: a re-emerging pathogen associated with occupational exposure to pigs or pork products. Part I – Epidemiology

    Directory of Open Access Journals (Sweden)

    Jacek Dutkiewicz

    2017-12-01

    Full Text Available Streptococcus suis (ex Elliot 1966, Kilpper-Bälz & Schleifer 1987 is a facultatively anaerobic Gram-positive ovoid or coccal bacterium surrounded by a polysaccharide capsule. Based on the antigenic diversity of the capsule, S. suis strains are classified serologically into 35 serotypes. Streptococcus suis is a commensal of pigs, commonly colonizing their tonsils and nasal cavities, mostly in weaning piglets between 4–10 weeks of age. This species occurs also in cattle and other mammals, in birds and in humans. Some strains, mostly those belonging to serotype 2, are also pathogenic for pigs, as well as for other animals and humans. Meningitis is the primary disease syndrome caused by S. suis , both in pigs and in humans. It is estimated that meningitis accounted for 68.0% of all cases of human disease reported until the end of 2012, followed by septicaemia (including life-threatening condition described as ‘streptococcal toxic shock-like syndrome’ – STSLS, arthritis, endocarditis, and endophthalmitis. Hearing loss and/or ves tibular dysfunction are the most common sequelae after recovery from meningitis caused by S. suis , occurring in more than 50% of patients. In the last two decades, the number of reported human cases due to S. suis has dramatically increased, mostly due to epidemics recorded in China in 1998 and 2005, and the fulminant increase in morbidity in the countries of south-eastern Asia, mostly Vietnam and Thailand. Out of 1,642 cases of S. suis infections identified between 2002–2013 worldwide in humans, 90.2% occurred in Asia, 8.5% in Europe and 1.3% in other parts of the globe. The human disease has mostly a zoonotic and occupational origin and occurs in pig breeders, abattoir workers, butchers and workers of meat processing facilities, veterinarians and meat inspectors. Bacteria are transmitted to workers by close contact with pigs or pig products, usually through contamination of minor cuts or abrasions on skin of

  2. Serotype- and virulence-associated gene profile of Streptococcus suis isolates from pig carcasses in Chiang Mai Province, Northern Thailand.

    Science.gov (United States)

    Wongsawan, Kanruethai; Gottschalk, Marcelo; Tharavichitkul, Prasit

    2015-02-01

    In this present study, the serotype of 40 Streptococcus suis isolates from submaxillary glands of pig carcasses sold in wet markets in Chiang Mai Province, northern Thailand, was investigated. Eleven serotypes, including types 2, 3, 4, 5, 7, 8, 9, 17, 21, 22 and 31, were found in the isolates by a Multiplex PCR combined with serum agglutination. Of the eleven serotypes present, type 3 was the most prevalent, while types 2, 4, 5 and 21 were of primary interest due to their human isolate serotype. The mrp+/epf - /sly - genotype was found to be the most prevalent genotype. This study indicates the importance of effective control of human S. suis infection due to raw pork or pig carcass handling in northern Thailand.

  3. Detection of Multiple Parallel Transmission Outbreak of Streptococcus suis Human Infection by Use of Genome Epidemiology, China, 2005.

    Science.gov (United States)

    Du, Pengcheng; Zheng, Han; Zhou, Jieping; Lan, Ruiting; Ye, Changyun; Jing, Huaiqi; Jin, Dong; Cui, Zhigang; Bai, Xuemei; Liang, Jianming; Liu, Jiantao; Xu, Lei; Zhang, Wen; Chen, Chen; Xu, Jianguo

    2017-02-01

    Streptococcus suis sequence type 7 emerged and caused 2 of the largest human infection outbreaks in China in 1998 and 2005. To determine the major risk factors and source of the infections, we analyzed whole genomes of 95 outbreak-associated isolates, identified 160 single nucleotide polymorphisms, and classified them into 6 clades. Molecular clock analysis revealed that clade 1 (responsible for the 1998 outbreak) emerged in October 1997. Clades 2-6 (responsible for the 2005 outbreak) emerged separately during February 2002-August 2004. A total of 41 lineages of S. suis emerged by the end of 2004 and rapidly expanded to 68 genome types through single base mutations when the outbreak occurred in June 2005. We identified 32 identical isolates and classified them into 8 groups, which were distributed in a large geographic area with no transmission link. These findings suggest that persons were infected in parallel in respective geographic sites.

  4. Assessing the zoonotic potential of Ascaris suum and Trichuris suis

    DEFF Research Database (Denmark)

    Nejsum, Peter; Betson, M.; Bendall, R. P.

    2012-01-01

    The two geohelminths, Ascaris lumbricoides and Trichuris trichiura, infect more than a billion people worldwide but are only reported sporadically in the developed part of the world. In contrast, the closely related species A. suum and T. suis in pigs have a truly global distribution, with infect...... and pig host, with special focus on recent evidence concerning the zoonotic potential of these parasites, and identify some open questions for future research....

  5. The Outcome of Repeated Mid Urethral Sling in SUI Treatment after Vaginal Excisions of Primary Failed Sling: Preliminary Study

    Directory of Open Access Journals (Sweden)

    Jacek Kociszewski

    2016-01-01

    Full Text Available Mid urethral sling is the standard in SUI treatment. Nevertheless, the risk of reoperation reaches 9%. There is no consensus as to the best treatment option for complications. A question is raised: what is the optimal way to achieve the best result in patients after primary failure? The aim of the study was to evaluate the outcomes of repeat MUS surgery in patients after excision of the sling with recurrent SUI. We compared its effectiveness with uncomplicated cases treated with TVT. 27 patients who underwent the repeated MUS and 50 consecutive patients after primary TVT were enrolled in the study. After 6 months, we have found that 24 (88.46% patients from repeat sling group and 48 (96% patients after primary sling were dry (1-hour pad test, 2 g or less. The difference between groups was not significant. We showed statistically significant improvement of quality of life in both groups. In conclusion, we showed that repeated sling after MUS excision is almost as effective as primary MUS. We postulate that sling excision and repeated MUS may be the best option for persistent SUI and/or complications after MUS procedures. Further multicenter observations are ongoing as to provide results on bigger group of cases.

  6. Virulence Studies of Different Sequence Types and Geographical Origins of Streptococcus suis Serotype 2 in a Mouse Model of Infection

    Directory of Open Access Journals (Sweden)

    Jean-Philippe Auger

    2016-07-01

    Full Text Available Multilocus sequence typing previously identified three predominant sequence types (STs of Streptococcus suis serotype 2: ST1 strains predominate in Eurasia while North American (NA strains are generally ST25 and ST28. However, ST25/ST28 and ST1 strains have also been isolated in Asia and NA, respectively. Using a well-standardized mouse model of infection, the virulence of strains belonging to different STs and different geographical origins was evaluated. Results demonstrated that although a certain tendency may be observed, S. suis serotype 2 virulence is difficult to predict based on ST and geographical origin alone; strains belonging to the same ST presented important differences of virulence and did not always correlate with origin. The only exception appears to be NA ST28 strains, which were generally less virulent in both systemic and central nervous system (CNS infection models. Persistent and high levels of bacteremia accompanied by elevated CNS inflammation are required to cause meningitis. Although widely used, in vitro tests such as phagocytosis and killing assays require further standardization in order to be used as predictive tests for evaluating virulence of strains. The use of strains other than archetypal strains has increased our knowledge and understanding of the S. suis serotype 2 population dynamics.

  7. Efficacy of sulfonamides and Baycox(®) against Isospora suis in experimental infections of suckling piglets.

    Science.gov (United States)

    Joachim, Anja; Mundt, Hans-Christian

    2011-12-01

    Sulfonamide treatment of piglets against neonatal coccidiosis has frequently been suggested in the literature. In order to evaluate the efficacy of sulfonamides against experimental Isospora suis infections in suckling piglets (oral infection with 1,500 sporulated oocysts of I. suis per piglet on the fourth day of life), two trials were conducted. In trial I, oral sulfadimidine (group Sulfa-Oral) was applied in doses of 100 mg/kg of body weight (BW) 1 day before infection and 75 mg/kg BW daily for the following 5 days, and sulfamethoxypyrimidine (SMP) was applied parenterally in daily doses of 75 mg/kg BW for the same time period. In trial II, SMP was applied parenterally in doses of 75 mg/kg BW (a) from the day of infection daily for 7 days (SMP-Standard), (b) for 2 days starting on the day of infection (SMP-Early), (c) for 3 days starting 2 days post-infection (d.p.i.; SMP-Middle), (d) for 2 days starting 5 d.p.i. (SMP-Late), and (e) every other day from the day of infection until 6 d.p.i. (SMP-Alternating), as well as (f) orally in doses of 75 mg/kg BW from the day of infection for 7 days (SMP-Oral). The sulfonamide-treated groups were compared to a toltrazuril-treated group (single oral treatment with Baycox® 5% suspension, 20 mg/kg BW 2 d.p.i.) and to a water-treated Control group. Each group consisted of seven to nine piglets. The parameters evaluated were oocyst excretion and fecal consistency/diarrhea from 4 to 15 d.p.i. Sulfa-Oral, SMP-Early, and SMP-Late had no significant effect in reduction of oocyst excretion and diarrhea, whereas treatment for 3-7 days with SMP reduced both parasite shedding and diarrhea significantly. Oral treatment with SMP was comparable to parenteral application. Baycox® in a single application had the most pronounced effect and completely suppressed oocyst excretion and diarrhea during the examination period. It could be shown that repeated application of sulfonamides, provided that the appropriate time period after infection

  8. Estatísticas sobre irrigação no Brasil segundo o Censo Agropecuário 1995-1996 Statistics on irrigation in Brazil according to the 1995-1996 Agricultural Census

    Directory of Open Access Journals (Sweden)

    Mardônio L. Loiol

    2001-04-01

    Full Text Available Neste trabalho utilizam-se dados do Censo Agropecuário 1995-1996, para se avaliar a irrigação no país, com relação à área irrigada, aos métodos utilizados e aos grupos de área, por região e por estado. A área irrigada no Brasil, de acordo com o Censo Agropecuário 1995-1996, é de 3,1 milhões de hectares, representando apenas 2,1% dos 146,8 milhões de hectares cultivados com lavouras permanentes, temporárias, pastagens e florestas. A região Sul apresenta a maior área irrigada, 1,1 milhão de ha, equivalente a 35% da área total irrigada. Em segundo lugar vem a região Sudeste com, aproximadamente, 30%; com 24% de toda a área irrigada no Brasil, o Nordeste ocupa a terceira posição seguido das regiões Centro-Oeste e Norte, que têm pouca expressão e, juntas, somam cerca de 11% do total. Nas regiões NE, SE e Sul, a irrigação predomina nos estabelecimentos cuja área é inferior a 1.000 ha. Diferentemente, nas regiões Norte e Centro-Oeste a irrigação é mais significativa nos estabelecimentos com área maior que 10.000 ha. O método de irrigação por superfície, continua a ser o mais utilizado no país (59%, nas regiões NE e Sul, com a aspersão prevalecendo nas demais regiões; a irrigação localizada representa a menor área.This paper uses the 1995-1996 of agricultural census data to evaluate irrigation in Brazil in relation to the irrigated areas and irrigation methods by groups, region and states. Based on this, Brazil has 3.1 million hectares under irrigation, corresponding to only 2.1% of the 146.8 million hectare of cultivated area with annual and permanent crops, pastures and forests. The southern region presents the largest irrigated area, 1.1 million ha, corresponding to 35% of the total irrigated area. The second largest irrigated area is in the southeastern region (30%, followed by the northeast (24%; the centre-western and north have, together by 11% of the total area. Irrigated farms smaller than 1

  9. [Expression, purification and protective antigen analysis of cell wall protein MRP of Streptococcus suis type 2].

    Science.gov (United States)

    Wang, Ping-ping; Pian, Ya-ya; Yuan, Yuan; Zheng, Yu-ling; Jiang, Yong-qiang; Xiong, Zheng-ying

    2012-02-01

    To amplify the mrp gene of Streptococcus suis type 2 05ZYH33, express it in E.coli BL21 in order to acquire high purity recombinant protein MRP, then evaluate the protective antigen of recombinant protein MRP. Using PCR technology to obtain the product of mrp gene of 05ZYH33, and then cloned it into the expression vector pET28a(+). The recombinant protein was purified by affinity chromatography, later immunized New Zealand rabbit to gain anti-serum, then test the anti-serum titer by ELISA. The opsonophagocytic killing test demonstrated the abilities of protective antigen of MRP. The truncated of MRP recombinant protein in E.coli BL21 expressed by inclusion bodies, and purified it in high purity. After immunoprotection, the survival condition of CD-1 was significantly elevated. The survival rate of wild-type strain 05ZYH33 in blood was apparently decreased after anti-serum opsonophagocyticed, but the mutant delta; MRP showed no differences. MRP represent an important protective antigen activity.

  10. Le prime sensazioni al pianoforte restano per sempre? Indagine sui metodi pianistici per principianti

    Directory of Open Access Journals (Sweden)

    Giuseppe Sellari

    2015-12-01

    Full Text Available I metodi d’insegnamento del pianoforte utilizzati in Italia sono caratterizzati da percorsi basati su differenti ipotesi teoriche e indicazioni pratiche. Il primo libro di pianoforte, qualunque sia il suo valore, stabilisce il primo contatto con lo strumento, lasciando una forte impressione e tracciando il profilo delle competenze inizialmente sviluppate. Una più profonda comprensione di questi testi può mostrare quali sono le capacità e le strategie di apprendimento proposte dai metodi adottati e conseguentemente dagli insegnanti. L'obiettivo di questo studio è quello di identificare quali sono le scelte metodologiche più rappresentative nella didattica pianistica italiana degli ultimi 50 anni. I risultati sono basati sui dati raccolti dai questionari somministrati a 200 pianisti, di età compresa tra 20 e 60, ai quali è stato chiesto, insieme a informazioni generiche, quale fosse stato il loro primo libro pianoforte, quali aspetti positivi o negativi ricordavano maggiormente e quali ritenevano essere più utili. I dati suggeriscono una preferenza rilevante di cinque metodi, che sono stati analizzati secondo una serie di criteri metodologici e analitici. I risultati indicano un panorama metodologico concentrato principalmente sul rapporto iniziale con lo strumento e sulla lettura musicale. Questo studio contribuisce alla riflessione su due aspetti metodologici: lo sviluppo di nuovi repertori e la proposta di metodologie in grado di rispettare il mondo emotivo e cognitivo dei principianti.

  11. Surveillance of antimicrobial resistance in clinical isolates of Pasteurella multocida and Streptococcus suis from Ontario swine

    Science.gov (United States)

    Glass-Kaastra, Shiona K.; Pearl, David L.; Reid-Smith, Richard J.; McEwen, Beverly; Slavic, Durda; Fairles, Jim; McEwen, Scott A.

    2014-01-01

    Susceptibility results for Pasteurella multocida and Streptococcus suis isolated from swine clinical samples were obtained from January 1998 to October 2010 from the Animal Health Laboratory at the University of Guelph, Guelph, Ontario, and used to describe variation in antimicrobial resistance (AMR) to 4 drugs of importance in the Ontario swine industry: ampicillin, tetracycline, tiamulin, and trimethoprim–sulfamethoxazole. Four temporal data-analysis options were used: visualization of trends in 12-month rolling averages, logistic-regression modeling, temporal-scan statistics, and a scan with the “What’s strange about recent events?” (WSARE) algorithm. The AMR trends varied among the antimicrobial drugs for a single pathogen and between pathogens for a single antimicrobial, suggesting that pathogen-specific AMR surveillance may be preferable to indicator data. The 4 methods provided complementary and, at times, redundant results. The most appropriate combination of analysis methods for surveillance using these data included temporal-scan statistics with a visualization method (rolling-average or predicted-probability plots following logistic-regression models). The WSARE algorithm provided interesting results for quality control and has the potential to detect new resistance patterns; however, missing data created problems for displaying the results in a way that would be meaningful to all surveillance stakeholders. PMID:25355992

  12. Possible pathogenic interplay between Chlamydia suis, Chlamydophila abortus and PCV-2 on a pig production farm.

    Science.gov (United States)

    Schautteet, K; Beeckman, D S A; Delava, P; Vanrompay, D

    2010-03-13

    A concurrent outbreak of chlamydial disease in boars, sows and gilts and postweaning multisystemic wasting syndrome (PMWS) in weaned piglets was investigated on a large pig production farm in Estonia. Chlamydia suis DNA was detected in conjunctival swabs from boars, sows and gilts, but also in the faeces of boars and sows. Chlamydophila abortus DNA was found in semen, and in conjunctival swabs from sows; DNA was demonstrated by microarrays. Serum samples from boars were examined using a Chlamydiaceae-specific recombinant ELISA. All 10 serum samples examined were positive (1:960 to 1:3840). Chlamydiosis was characterised by reproductive failure and conjunctivitis. Piglets were not examined for Chlamydiaceae, as eye problems were not observed. Piglets showed wasting, respiratory signs, diarrhoea, enlargement of lymph nodes and increased mortality (10 per cent). Porcine circovirus type 2 (PCV-2) was detected in the lymph nodes of piglets by immunohistochemistry, and PCV-2 antibodies were demonstrated in all 10 serum samples from sows examined using an immunoperoxidase monolayer assay.

  13. In vitro sensitivity of Hungarian Actinobaculum suis strains to selected antimicrobials.

    Science.gov (United States)

    Biksi, I; Major, Andrea; Fodor, L; Szenci, O; Vetési, F

    2003-01-01

    In vitro antimicrobial sensitivity of 12 Hungarian isolates and the type strain ATCC 33144 of Actinobaculum suis to different antimicrobial compounds was determined both by the agar dilution and by the disc diffusion method. By agar dilution, MIC50 values in the range of 0.05-3.125 micrograms/ml were determined for penicillin, ampicillin, ceftiofur, doxycycline, tylosin, pleuromutilins, chloramphenicol, florfenicol, enrofloxacin and lincomycin. The MIC50 value of oxytetracycline and spectinomycin was 6.25 and 12.5 micrograms/ml, respectively. For ofloxacin, flumequine, neomycin, streptomycin, gentamicin, nalidixic acid, nitrofurantoin and sulphamethoxazole + trimethoprim MIC50 values were in the range of 25-100 micrograms/ml. With the disc diffusion method, all strains were sensitive to penicillin, cephalosporins examined, chloramphenicol and florfenicol, tetracyclines examined, pleuromutilins, lincomycin and tylosin. Variable sensitivity was observed for fluoroquinolones (flumequine, enrofloxacin, ofloxacin), most of the strains were susceptible to marbofloxacin. Almost all strains were resistant to aminoglycosides but most of them were sensitive to spectinomycin. A strong correlation was determined for disc diffusion and MIC results (Spearman's rho 0.789, p < 0001). MIC values of the type strain and MIC50 values of other tested strains did not differ significantly. Few strains showed a partially distinct resistance pattern for erythromycin, lincomycin and ampicillin in both methods.

  14. Streptococcus suis Serotype 2 Biofilms Inhibit the Formation of Neutrophil Extracellular Traps.

    Science.gov (United States)

    Ma, Fang; Yi, Li; Yu, Ningwei; Wang, Guangyu; Ma, Zhe; Lin, Huixing; Fan, Hongjie

    2017-01-01

    Invasive infections caused by Streptococcus suis serotype 2 (SS2) has emerged as a clinical problem in recent years. Neutrophil extracellular traps (NETs) are an important mechanism for the trapping and killing of pathogens that are resistant to phagocytosis. Biofilm formation can protect bacteria from being killed by phagocytes. Until now, there have only been a few studies that focused on the interactions between bacterial biofilms and NETs. SS2 in both a biofilm state and a planktonic cell state were incubated with phagocytes and NETs, and bacterial survival was assessed. DNase I and cytochalasin B were used to degrade NET DNA or suppress phagocytosis, respectively. Extracellular DNA was stained with impermeable fluorescent dye to quantify NET formation. Biofilm formation increased up to 6-fold in the presence of neutrophils, and biofilms were identified in murine tissue. Both planktonic and biofilm cells induced neutrophils chemotaxis to the infection site, with neutrophils increasing by 85.1 and 73.8%, respectively. The bacteria in biofilms were not phagocytized. The bactericidal efficacy of NETs on the biofilms and planktonic cells were equal; however, the biofilm extracellular matrix can inhibit NET release. Although biofilms inhibit NETs release, NETs appear to be an important mechanism to eliminate SS2 biofilms. This knowledge advances the understanding of biofilms and may aid in the development of treatments for persistent infections with a biofilm component.

  15. New Features in the Lipid A Structure of Brucella suis and Brucella abortus Lipopolysaccharide

    Science.gov (United States)

    Casabuono, Adriana C.; Czibener, Cecilia; Del Giudice, Mariela G.; Valguarnera, Ezequiel; Ugalde, Juan E.; Couto, Alicia S.

    2017-12-01

    Brucellaceae are Gram-negative bacteria that cause brucellosis, one of the most distributed worldwide zoonosis, transmitted to humans by contact with either infected animals or their products. The lipopolysaccharide exposed on the cell surface has been intensively studied and is considered a major virulence factor of Brucella. In the last years, structural studies allowed the determination of new structures in the core oligosaccharide and the O-antigen of this lipopolysaccharide. In this work, we have reinvestigated the lipid A structure isolated from B. suis and B. abortus lipopolysaccharides. A detailed study by MALDI-TOF mass spectrometry in the positive and negative ion modes of the lipid A moieties purified from both species was performed. Interestingly, a new feature was detected: the presence of a pyrophosphorylethanolamine residue substituting the backbone. LID-MS/MS analysis of some of the detected ions allowed assurance that the Lipid A structure composed by the diGlcN3N disaccharide, mainly hexa-acylated and penta-acylated, bearing one phosphate and one pyrophosphorylethanolamine residue. [Figure not available: see fulltext.

  16. [Isolation and identification of the temperate bacteriophage from isolated strains of Streptococcus suis serotype 2].

    Science.gov (United States)

    Ma, Yuling; Lu, Chengping; Fan, Hongjie

    2008-04-01

    A PCR assay was developed to study the distributional characteristics of phage integrase gene in Streptococcus suis serotype 2 (SS2). A 323bp distinct DNA target can be amplified in 25 strains of virulent SS2, while can not be amplified in avirulent strain T15, 5 strains of other serotypes (SS1, SS7, SS9) and strains of group C Streptococcus strains from pigs, which suggested that the phage integrase gene may be related to the pathogenicity of SS2 and can be consider as a detection factor of the virulent gene of SS2. The sequencing and restriction endonuclease analysis of the PCR products were also done. Comparisons between the sequences of phage integrase gene with that of SS2 strain, showed a high homology with SS2 China strains 98HAH33, 05ZYH33 and North American strain 89-1591. Complete cell lysis was observed with SS2 virulent strains but not with avirulent strain T15 after the induction by mitomycin C. Electron microscopy analysis of the lysate from SS2 virulent strains HA9801 and ZY05719 revealed the presence of phage particles. The induced phage, named SS2-HA and SS2-ZY, both have a small isometric nucleocapsid approximately 50 nm in diameter and have no tail and is therefore a member of the Tectiviridae family. The phage integrase gene sequence of phage SS2-HA and SS2-ZY shared high homologue identities with virulent SS2 strains, which suggested that the phage integrase gene of SS2 has high specify. The temperate phage and phage integrase gene can only detected from SS2 virulent strains but not from avirulent strain, and the detection of phage integrase gene was related to the virulence-associate factors of SS2, such as the muramidase-released protein gene (mrp), which suggested that the temperate phage of SS2 may be related to the pathogenicity of SS2.

  17. Probing genomic diversity and evolution of Streptococcus suis serotype 2 by NimbleGen tiling arrays

    Directory of Open Access Journals (Sweden)

    Liao Hui

    2011-05-01

    Full Text Available Abstract Background Our previous studies revealed that a new disease form of streptococcal toxic shock syndrome (STSS is associated with specific Streptococcus suis serotype 2 (SS2 strains. To achieve a better understanding of the pathogenicity and evolution of SS2 at the whole-genome level, comparative genomic analysis of 18 SS2 strains, selected on the basis of virulence and geographic origin, was performed using NimbleGen tiling arrays. Results Our results demonstrate that SS2 isolates have highly divergent genomes. The 89K pathogenicity island (PAI, which has been previously recognized as unique to the Chinese epidemic strains causing STSS, was partially included in some other virulent and avirulent strains. The ABC-type transport systems, encoded by 89K, were hypothesized to greatly contribute to the catastrophic features of STSS. Moreover, we identified many polymorphisms in genes encoding candidate or known virulence factors, such as PlcR, lipase, sortases, the pilus-associated proteins, and the response regulator RevS and CtsR. On the basis of analysis of regions of differences (RDs across the entire genome for the 18 selected SS2 strains, a model of microevolution for these strains is proposed, which provides clues into Streptococcus pathogenicity and evolution. Conclusions Our deep comparative genomic analysis of the 89K PAI present in the genome of SS2 strains revealed details into how some virulent strains acquired genes that may contribute to STSS, which may lead to better environmental monitoring of epidemic SS2 strains.

  18. Genetic analysis of Trichuris suis and Trichuris trichiura recovered from humans and pigs in a sympatric setting in Uganda

    DEFF Research Database (Denmark)

    Nissen, Sofie; Al-Jubury, Azmi; Hansen, Tina Vicky Alstrup

    2012-01-01

    The whipworms Trichuris trichiura and Trichuris suis in humans and pigs, respectively, are believed to be two different species yet closely related. Morphologically, adult worms, eggs and larvae of the two species are indistinguishable. The aim of this study was to examine the genetic variation....... suis-type) and three cases of ‘heterozygote’ worms in humans were identified. However, the analysis showed that sympatric Trichuris primarily assorted with host origin. Sequence analysis of a part of the genetically conserved ¿-tubulin gene confirmed two separate populations/species but also showed...... that the ‘heterozygote’ worms had a T. suis-like ¿-tubulin gene. A PCR-RFLP on the ITS-2 region was developed, that could distinguish between worms of the pig, human and ‘heterozygote’ type. The data suggest that Trichuris in pigs and humans belong to two different populations (i.e. are two different species). However...

  19. Characterization of a Streptococcus suis tet(O/W/32/O)-carrying element transferable to major streptococcal pathogens.

    Science.gov (United States)

    Palmieri, Claudio; Magi, Gloria; Mingoia, Marina; Bagnarelli, Patrizia; Ripa, Sandro; Varaldo, Pietro E; Facinelli, Bruna

    2012-09-01

    Mosaic tetracycline resistance determinants are a recently discovered class of hybrids of ribosomal protection tet genes. They may show different patterns of mosaicism, but their final size has remained unaltered. Initially thought to be confined to a small group of anaerobic bacteria, mosaic tet genes were then found to be widespread. In the genus Streptococcus, a mosaic tet gene [tet(O/W/32/O)] was first discovered in Streptococcus suis, an emerging drug-resistant pig and human pathogen. In this study, we report the molecular characterization of a tet(O/W/32/O) gene-carrying mobile element from an S. suis isolate. tet(O/W/32/O) was detected, in tandem with tet(40), in a circular 14,741-bp genetic element (39.1% G+C; 17 open reading frames [ORFs] identified). The novel element, which we designated 15K, also carried the macrolide resistance determinant erm(B) and an aminoglycoside resistance four-gene cluster including aadE (streptomycin) and aphA (kanamycin). 15K appeared to be an unstable genetic element that, in the absence of recombinases, is capable of undergoing spontaneous excision under standard growth conditions. In the integrated form, 15K was found inside a 54,879-bp integrative and conjugative element (ICE) (50.5% G+C; 55 ORFs), which we designated ICESsu32457. An ∼1.3-kb segment that apparently served as the att site for excision of the unstable 15K element was identified. The novel ICE was transferable at high frequency to recipients from pathogenic Streptococcus species (S. suis, Streptococcus pyogenes, Streptococcus pneumoniae, and Streptococcus agalactiae), suggesting that the multiresistance 15K element can successfully spread within streptococcal populations.

  20. Long-term outcomes of patients with Streptococcus suis infection in Viet Nam: A case-control study.

    Science.gov (United States)

    Huong, Vu T L; Long, Hoang B; Kinh, Nguyen V; Ngan, Ta T D; Dung, Vu T V; Nadjm, Behzad; van Doorn, H Rogier; Hoa, Ngo T; Horby, Peter; Wertheim, Heiman F L

    2018-02-01

    Streptococcus suis is a zoonotic cause of severe meningitis and sepsis in humans. We aimed to assess the long-term outcomes in patients who survived S. suis infection, in particular the progress and impact of vestibulocochlear sequelae. This case-control study evaluated outcomes of S. suis infection at discharge and 3 and 9 months post-discharge for 47 prospectively enrolled cases and at 11-34 months for 31 retrospectively enrolled cases. Outcomes in patients were compared to 270 controls matched for age, sex and residency. The prevalence ratio (PR) of moderate-to-complete hearing loss was 5.0(95%CI 3.6-7.1) in cases at discharge, 3.7(2.5-5.4) at 3 months, 3.2(2.2-4.7) at 9 months, and 3.1(2.1-4.4) in retrospective cases compared to controls. Hearing improvement occurred mostly within the first 3 months with a change in hearing level of 11.1%(95%CI 7.0-15.1%) compared to discharge. The PR of vestibular dysfunction was 2.4(95%CI 1.7-3.3) at discharge, 2.2(1.4-3.1) at 3 months, 1.8(1.1-2.5) at 9 months, and 1.8(1.1-2.6) for retrospective cases compared to controls. Cases also indicated more problems with mobility, self-care and usual activities. Both hearing and vestibular impairment were common and persist in cases. Appropriate patient management strategies are needed to reduce the incidence and impact of these sequelae. Copyright © 2017 The Authors. Published by Elsevier Ltd.. All rights reserved.

  1. Parasite-specific IL-4 responses in Ascaris suum and Trichuris suis-infected pigs evaluated by ELISPOT

    DEFF Research Database (Denmark)

    Steenhard, N. R.; Kringel, H.; Roepstorff, A.

    2007-01-01

    method is a valuable tool for future experimental settings as it enables repeated and parasite-specific measurement of IL-4 at protein level when investigating, for example, immunomodulatory properties of helminths. Furthermore, the method could be used to identify specific parasite antigens inducing IL......The objective of the present study was to develop an ELISPOT method to measure parasite-specific IL-4 producing cells during experimental Ascaris suum and Trichuris suis infections in pigs. In many experimental settings it is useful to be able to measure changes in specifically induced cytokines...

  2. Efficacy of Antimicrobial Treatments and Vaccination Regimens for Control of Porcine Reproductive and Respiratory Syndrome Virus and Streptococcus suis Coinfection of Nursery Pigs

    Science.gov (United States)

    Halbur, P.; Thanawongnuwech, R.; Brown, G.; Kinyon, J.; Roth, J.; Thacker, E.; Thacker, B.

    2000-01-01

    Seventy-six, crossbred, porcine reproductive and respiratory syndrome virus (PRRSV)-free pigs were weaned at 12 days of age and randomly assigned to seven groups of 10 to 11 pigs each. Pigs in group 1 served as unchallenged controls. Pigs in groups 2 to 7 were challenged intranasally with 2 ml of high-virulence PRRSV isolate VR-2385 (104.47 50% tissue culture infective doses per 2 ml) on day 0 of the study (30 days of age). Seven days after PRRSV challenge, pigs in groups 2 to 7 were challenged intranasally with 2 ml of Streptococcus suis serotype 2 (108.30 CFU/2 ml). Group 2 pigs served as untreated positive controls. Antimicrobial treatments included daily intramuscular injection with 66,000 IU of procaine penicillin G per kg of body weight on days 8 to 10 (group 3), drinking water medication with 23.1 mg of tiamulin per kg during days 8 to 10 (group 4), and daily intramuscular injection of 5.0 mg of ceftiofur hydrochloride per kg on days 8 to 10 (group 5). Vaccination regimens included two intramuscular doses of an autogenous killed S. suis vaccine (group 6) prior to S. suis challenge or a single 2-ml intramuscular dose of an attenuated live PRRSV vaccine (group 7) 2 weeks prior to PRRSV challenge. Mortality was 0, 63, 45, 54, 9, 40, and 81% in groups 1 to 7, respectively. Ceftiofur treatment was the only regimen that significantly (P < 0.05) reduced mortality associated with PRRSV and S. suis coinfection. The other treatments and vaccinations were less effective. We conclude that ceftiofur administered by injection for three consecutive days following S. suis challenge was the most effective regimen for minimizing disease associated with PRRSV and S. suis coinfection. PMID:10699012

  3. Clinical utility of the MMPI-2-RF SUI items and scale in a forensic inpatient setting: Association with interview self-report and future suicidal behaviors.

    Science.gov (United States)

    Glassmire, David M; Tarescavage, Anthony M; Burchett, Danielle; Martinez, Jennifer; Gomez, Anthony

    2016-11-01

    In this study, we examined whether the 5 Minnesota Multiphasic Personality Inventory-2-Restructured Form (MMPI-2-RF; Ben-Porath & Tellegen, 2008/2011) Suicidal/Death Ideation (SUI) items (93, 120, 164, 251, and 334) would provide incremental suicide-risk assessment information after accounting for information garnered from clinical interview questions. Among 229 forensic inpatients (146 men, 83 women) who were administered the MMPI-2-RF, 34.9% endorsed at least 1 SUI item. We found that patients who endorsed SUI items on the MMPI-2-RF concurrently denied conceptually related suicide-risk information during the clinical interview. For instance, 8% of the sample endorsed Item 93 (indicating recent suicidal ideation), yet denied current suicidal ideation upon interview. Conversely, only 2.2% of the sample endorsed current suicidal ideation during the interview, yet denied recent suicidal ideation on Item 93. The SUI scale, as well as the MMPI-2-RF Demoralization (RCd) and Low Positive Emotions (RC2) scales, correlated significantly and meaningfully with conceptually related suicide-risk information from the interview, including history of suicide attempts, history of suicidal ideation, current suicidal ideation, and months since last suicide attempt. We also found that the SUI scale added incremental variance (after accounting for information garnered from the interview and after accounting for scores on RCd and RC2) to predictions of future suicidal behavior within 1 year of testing. Relative risk ratios indicated that both SUI-item endorsement and the presence of interview-reported risk information significantly and meaningfully increased the risk of suicidal behavior in the year following testing, particularly when endorsement of suicidal ideation occurred for both methods of self-report. (PsycINFO Database Record (c) 2016 APA, all rights reserved).

  4. Evolution and Diversity of the Antimicrobial Resistance Associated Mobilome in Streptococcus suis: A Probable Mobile Genetic Elements Reservoir for Other Streptococci.

    Science.gov (United States)

    Huang, Jinhu; Ma, Jiale; Shang, Kexin; Hu, Xiao; Liang, Yuan; Li, Daiwei; Wu, Zuowei; Dai, Lei; Chen, Li; Wang, Liping

    2016-01-01

    Streptococcus suis is a previously neglected, newly emerging multidrug-resistant zoonotic pathogen. Mobile genetic elements (MGEs) play a key role in intra- and interspecies horizontal transfer of antimicrobial resistance (AMR) determinants. Although, previous studies showed the presence of several MGEs, a comprehensive analysis of AMR-associated mobilome as well as their interaction and evolution has not been performed. In this study, we presented the AMR-associated mobilome and their insertion hotspots in S. suis . Integrative conjugative elements (ICEs), prophages and tandem MGEs were located at different insertion sites, while 86% of the AMR-associated MGEs were inserted at rplL and rum loci. Comprehensive analysis of insertions at rplL and rum loci among four pathogenic Streptococcus species ( Streptococcus agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes , and S. suis ) revealed the existence of different groups of MGEs, including Tn5252, ICE Sp 1108, and TnGBS2 groups ICEs, Φm46.1 group prophage, ICE_ICE and ICE_prophage tandem MGEs. Comparative ICE genomics of ICE Sa 2603 family revealed that module exchange and acquisition/deletion were the main mechanisms in MGEs' expansion and evolution. Furthermore, the observation of tandem MGEs reflected a novel mechanism for MGE diversity. Moreover, an in vitro competition assay showed no visible fitness cost was observed between different MGE-carrying isolates and a conjugation assay revealed the transferability of ICE Sa 2603 family of ICEs. Our statistics further indicated that the prevalence and diversity of MGEs in S. suis is much greater than in other three species which prompted our hypothesis that S. suis is probably a MGEs reservoir for other streptococci. In conclusion, our results showed that acquisition of MGEs confers S. suis not only its capability as a multidrug resistance pathogen, but also represents a paradigm to study the modular evolution and matryoshkas of MGEs.

  5. Evolution and diversity of the antimicrobial resistance associated mobilome in Streptococcus suis: a probable mobile genetic elements reservoir for other streptococci

    Directory of Open Access Journals (Sweden)

    Jinhu Huang

    2016-10-01

    Full Text Available Streptococcus suis is a previously neglected, newly emerging multidrug-resistant zoonotic pathogen. Mobile genetic elements (MGEs play a key role in intra- and interspecies horizontal transfer of antimicrobial resistance (AMR determinants. Although previous studies showed the presence of several MGEs, a comprehensive analysis of AMR-associated mobilome as well as their interaction and evolution has not been performed. In this study, we presented the AMR-associated mobilome and their insertion hotspots in S. suis. Integrative conjugative elements (ICEs, prophages and tandem MGEs were located at different insertion sites, while 86% of the AMR-associated MGEs were inserted at rplL and rum loci. Comprehensive analysis of insertions at rplL and rum loci among four pathogenic Streptococcus species (Streptococcus agalactiae, Streptococcus pneumoniae, Streptococcus pyogenes, and S. suis revealed the existence of different groups of MGEs, including Tn5252, ICESp1108, and TnGBS2 groups ICEs, Φm46.1 group prophage, ICE_ICE and ICE_prophage tandem MGEs. Comparative ICE genomics of ICESa2603 family revealed that module exchange and acquisition/deletion were the main mechanisms in MGEs’ expansion and evolution. Furthermore, the observation of tandem MGEs reflected a novel mechanism for MGE diversity. Moreover, an in vitro competition assay showed no visible fitness cost was observed between different MGE-carrying isolates and a conjugation assay revealed the transferability of ICESa2603 family of ICEs. Our statistics further indicated that the prevalence and diversity of MGEs in S. suis is much greater than in other three species which prompted our hypothesis that S. suis is probably a MGEs reservoir for other streptococci. In conclusion, our results showed that acquisition of MGEs confers S. suis not only its capability as a multidrug resistance pathogen, but also represents a paradigm to study the modular evolution and matryoshkas of MGEs.

  6. Development of a Chlamydia suis-specific antibody enzyme-linked immunosorbent assay based on the use of a B-cell epitope of the polymorphic membrane protein C.

    Science.gov (United States)

    De Puysseleyr, K; Kieckens, E; De Puysseleyr, L; Van den Wyngaert, H; Ahmed, B; Van Lent, S; Creasy, H H; Myers, G S A; Vanrompay, D

    2018-04-01

    Chlamydia suis infections lead to economic loss in the pork industry. Chlamydia suis infections could be successfully treated with tetracyclines until the appearance of a tetracycline resistant phenotype, which was acquired via horizontal gene transfer of the tet(C) gene. Given the importance of C. suis as a swine pathogen and as a recently emerged tetracycline resistant pathogen with zoonotic potential, our aim was to develop a sensitive C. suis-specific antibody ELISA based on the polymorphic membrane proteins (Pmps). Chlamydia Pmps are important virulence factors and candidate antigens for serodiagnosis. We identified nine Pmps (PmpA to I) in C. suis strain MD56 using a recently developed Hidden-Markov model. PmpC was the most promising candidate for the development of a C. suis-specific antibody ELISA as the protein was absent in C. abortus, C. pecorum and C. psittaci which also infect pigs and as the protein contained C. suis-specific amino acid regions, absent in C. trachomatis PmpC. We identified an immunodominant B-cell epitope in C. suis PmpC using experimental porcine sera. The sensitivity and specificity of the PmpC ELISA was compared to the complement fixation test (CFT) and to a recombinant MOMP ELISA using experimental sera. The PmpC ELISA detected all positive control sera and was in contrast to CFT and the rMOMP ELISA 100% C. suis specific as positive control sera against other Chlamydia species did not react in the PmpC ELISA. The test was successfully validated using slaughterhouse sera and sera from clinically affected pigs. The PmpC ELISA could assist in diminishing the spread of C. suis infections in the pork industry. © 2018 Blackwell Verlag GmbH.

  7. A escolarização das pessoas com deficiência no Brasil: atendimento, atraso e progressão no ensino fundamental segundo o Censo 2000 La escolarización de personas con discapacidad en Brasil: atención, atraso y progresión en la enseñanza fundamental según el Censo 2000 The schooling of disabled people in Brazil: attendance, retention and progression in primary education according to the 2000 Census

    Directory of Open Access Journals (Sweden)

    Tiago Henrique P. M. França

    2012-12-01

    Full Text Available O presente artigo tem por objetivo evidenciar a implicação das deficiências sobre o atendimento, o atraso e a progressão escolar no ensino fundamental. Para tal, é utilizada a modelagem estatística logística binária. Após apresentação dos diferentes conceitos de deficiência, por meio do Censo 2000, transcorre-se a análise dos quesitos pretendidos. A progressão escolar é pesquisada por meio da medida nomeada probabilidade de progressão por série (PPS, dos três pontos de maior relevância no desenvolvimento do ensino fundamental. Os resultados indicam grande disparidade entre as deficiências na determinação dos elementos investigados. Em geral, todas as condições se mostram como fatores que desfavorecem o desenvolvimento na carreira escolar. Porém, a implicação das deficiências sobre as PPS se reduz com o avanço no ensino fundamental, sendo também menos significativa a cada etapa sequente. Entre os quesitos analisados, o atraso escolar demonstrou sofrer relevante variação, devido ao controle por fatores de natureza socioeconômica. No mais, este trabalho contém resultados e aponta contributos para o desenvolvimento científico dessa área de estudo.El presente artículo tiene como objetivo poner en evidencia la implicación de las diferentes discapacidades en áreas como la atención, el atraso y la progresión escolar en la enseñanza fundamental. Para ello, se utiliza el modelo estadístico logístico binario. Tras la presentación de los diferentes conceptos de discapacidad, usando como instrumento el Censo 2000, transcurre el análisis de las áreas mencionadas anteriormente. La progresión escolar se investiga por medio de una medida denominada Probabilidad de Progresión por Clase11 (PPS, tratándose, además, de uno de los tres puntos de mayor relevancia en el desarrollo de la enseñanza fundamental. Los resultados indican una gran disparidad entre las discapacidades en la determinación de los elementos

  8. Aberrant chlamydial developmental forms in the gastrointestinal tract of pigs spontaneously and experimentally infected with Chlamydia suis.

    Science.gov (United States)

    Pospischil, Andreas; Borel, Nicole; Chowdhury, Emdad H; Guscetti, Franco

    2009-03-16

    The phenomenon of persistence is well known from in vitro studies, where it is associated with the production of aberrant bodies, but its occurrence in vivo is less well documented. The objective of this study was to search for aberrant bodies in intestinal tissues from pigs, describe their ultrastructure, and investigate the suitability of immunohistochemical staining for chlamydial heat shock protein 60 (cHSP60) to detect such forms. Intestinal tissues derived from pigs naturally and experimentally infected with Chlamydia (C.) suis were examined by immunohistochemistry, transmission electron microscopy and immunogold electron microscopy. The chlamydial species involved in the natural infection were determined using an Array Tube Microarray to C. suis and Chlamydophila abortus. Ultrastructurally, aberrant bodies were detected in the gut of both naturally and experimentally infected pigs. Immunogold electron microscopy showed that the aberrant bodies were labeled less strongly than the normal forms by antibodies against LPS and cHSP60 respectively. It was concluded that aberrant bodies occur in vivo in pigs and that the gnotobiotic pig model might be suitable for the study of chlamydial persistence in vivo. The antibody against cHSP60 does not appear to be suitable to specifically detect such forms.

  9. Multifunctional Thioredoxin-Like Protein from the Gastrointestinal Parasitic Nematodes Strongyloides ratti and Trichuris suis Affects Mucosal Homeostasis

    Directory of Open Access Journals (Sweden)

    Dana Ditgen

    2016-01-01

    Full Text Available The cellular redox state is important for the regulation of multiple functions and is essential for the maintenance of cellular homeostasis and antioxidant defense. In the excretory/secretory (E/S products of Strongyloides ratti and Trichuris suis sequences for thioredoxin (Trx and Trx-like protein (Trx-lp were identified. To characterize the antioxidant Trx-lp and its interaction with the parasite’s mucosal habitat, S. ratti and T. suis Trx-lps were cloned and recombinantly expressed. The primary antioxidative activity was assured by reduction of insulin and IgM. Further analysis applying an in vitro mucosal 3D-cell culture model revealed that the secreted Trx-lps were able to bind to monocytic and intestinal epithelial cells and induce the time-dependent release of cytokines such as TNF-α, IL-22, and TSLP. In addition, the redox proteins also possessed chemotactic activity for monocytic THP-1 cells and fostered epithelial wound healing activity. These results confirm that the parasite-secreted Trx-lps are multifunctional proteins that can affect the host intestinal mucosa.

  10. ANALYSIS OF REMOTE SENSING ARCHAEOLOGY ON TRAFFIC FUNCTION TRANSFORMATION OF TONGJI GRAND CANAL IN SUI AND TANG DYNASTIES

    Institute of Scientific and Technical Information of China (English)

    WANG Xin-yuan; HE Hui; ZHOU Ying-qiu; GAO Chao; HAN Shuang-wang

    2006-01-01

    In China, most directions of river flowing are from west to east. During historic period, since the water traffic played an important role, it was very important to form a cross-horizontal net of water carriage route. Canals should be dug so as to make up the lack of north-south river. Tongji Grand Canal, dug in the first year of Daye (605 A.D.) in the Sui Dynasty, was the important component of north-south system of Grand Canals in China. It promoted economic and social development of the Sui, Tang and Song dynasties (605 A.D.-1279 A.D.). As Tongji Canal (i.e.Tongji Grand Canal) flowed across the Huaibei Plain, which is aggraded by abundant mud and sand deposit resulted from the Huanghe (Yellow) River flooding, many traces (such as old channel) and human culture heritages were buried under mud-sand. Tongji Canal was silted up, and disappeared in the Jin Dynasty (1115 A.D.-1234 A.D.). From then on, there were many different stories about the flowing route of the canal in historical literature. Based on space-bone and air-bone remote sensing imagery, we attempt to search the old channel of Tongji Canal, and supplement historical record. The paper discusses transformation process of Tongji Canal's traffic function, and resumptively summarizes the reasons of the transformation, which results from synthetic function of physical geographical, political, economic, and social conditions.

  11. [Bladder injury during sling operation in the treatment of SUI--review of literature and case report].

    Science.gov (United States)

    Gałczyński, Krzysztof; Futyma, Konrad; Bar, Krzysztof; Rechberger, Tomasz

    2012-10-01

    Sling operations have been performed for over 15 years. In recent years these operations have become the gold standard in the treatment of stress urinary incontinence (SUI) due to their efficacy safety and low invasiveness. Approximately 4% of women will undergo a surgery for SUI in the course of their life. As with any surgical intervention, there may be some technical problems, as well as intra- and postoperative complications, the most common of which is bladder injury Other complications encountered during mid-urethral slings procedures include bleeding (retropubic or vaginal hematomas), urethral perforation, urinary tract infections, postoperative vaginal or urethral erosions, bowel perforation, chronic pelvic pain, wound infection, nerve injury transient and persistent voiding dysfunction such as de novo urgency incomplete bladder emptying or urinary retention. Below we present a case of a patient with diagnosed vesicovaginal fistula after sling operation (TVT-tension-free vaginal tape). Upon admission the patient reported dysuria, persistent urinary leakage and abnormal, abundant vaginal discharge. Case report and review of literature concerning surgical treatment of stress urinary incontinence and its complications. Analysis of medical documentation of the patient treated at the Second Department of Gynecology Medical University of Lublin. Review of abstracts or papers in the Medline database related to surgical treatment of urinary incontinence and its complications. Bladder perforation is one of the most common complications of the retropubic approach for MUS placement. The presence of mesh within the bladder may arise from direct bladder perforation or from subsequent erosion of the sling. Such lesions do not cause any serious health consequences for patients on condition they are detected intraoperatively and appropriately repaired, but when unrecognized, they results in the development of considerable symptoms and negatively influence the quality of

  12. Explicando as diferenças de pobreza entre produtores agrícolas no Brasil: simulações contrafactuais com o censo agropecuário 1995-96

    Directory of Open Access Journals (Sweden)

    Steven M. Helfand

    2011-06-01

    Full Text Available O censo agropecuário 1995-96 é utilizado para estudar os fatores responsáveis pelas diferenças na pobreza entre os estabelecimentos agrícolas no Brasil. As diferenças entre as regiões e as seguintes dicotomias são analisadas: proprietários/não proprietários; familiares/não familiares; intensivos em insumos/não intensivos; com/sem máquinas. O artigo utiliza uma metodologia semiparamétrica. Primeiro, uma função fronteira estocástica de lucro é estimada. Em seguida, simulações não paramétricas contrafactuais são utilizadas para identificar os fatores que explicam as diferenças em pobreza. Os fatores incluem o tamanho da propriedade, o trabalho familiar, a lucratividade e as variáveis utilizadas para explicar a lucratividade no modelo de fronteira estocástica. O artigo conclui que a falta de terra e os baixos níveis de produtividade são importantes motivos para a pobreza entre produtores agrícolas. É improvável que qualquer um deles isoladamente possa solucionar o problema da pobreza. As diferenças em "eficiência" - capturadas pelo termo de erro na função fronteira de lucro - são importantes para explicar as diferenças entre os pobres e os não pobres, mas não para as demais dicotomias estudadas. Isto sugere que os fatores não observáveis - como restrições ou falhas de mercado - são importantes para explicar a pobreza entre produtores agrícolas no Brasil.The 1995-96 agricultural census is used to explore factors that account for differences in poverty among agricultural establishments in Brazil. Differences across regions and the following dichotomies are analyzed: owners/non-owners, family/non-family, input intensive/non-intensive, with machines/without machines. The paper uses a semi-parametric methodology. First, a stochastic frontier profit function is estimated. Then, non-parametric counterfactual simulations are used to identify factors which explain differences in poverty. Factors include farm size

  13. Protection of pigs against challenge with virulent Streptococcus suis serotype 2 strains by a muramidase-released protein and extracellular factor vaccine

    NARCIS (Netherlands)

    Wisselink, H.J.; Vecht, U.; Stockhofe Zurwieden, N.; Smith, H.E.

    2001-01-01

    The efficacy of a muramidase-released protein (MRP) and extracellular factor (EF) vaccine in preventing infection and disease in pigs challenged either with a homologous or a heterologous Streptococcus suis serotype 2 strain (MRP EF ) was compared with the efficacy of a vaccine containing

  14. Identification and characterization of two temperature-induced surface-associated proteins of Streptococcus suis with high homologies to members of the arginine deiminase system of Streptococcus pyogenes

    NARCIS (Netherlands)

    Winterhoff, N.; Goethe, R.; Gruening, P.; Rohde, M.; Kalisz, H.; Smith, H.E.; Valentin-Weigand, P.

    2002-01-01

    The present study was performed to identify stress-induced putative virulence proteins of Streptococcus suis. For this, protein expression patterns of streptococci grown at 32, 37, and 42°C were compared by one- and two-dimensional gel electrophoresis. Temperature shifts from 32 and 37 to 42°C

  15. Quantitative susceptibility of Streptococcus suis strains isolated from diseased pigs in seven European countries to antimicrobial agents licenced in veterinary medicine

    NARCIS (Netherlands)

    Wisselink, H.J.; Veldman, K.T.; Salmon, S.A.; Mevius, D.J.

    2006-01-01

    The susceptibility of Streptococcus suis strains (n = 384) isolated from diseased pigs in seven European countries to 10 antimicrobial agents was determined. For that purpose a microbroth dilution method was used according to CLSI recommendations. The following antimicrobial agents were tested:

  16. Salmonella enterica serovar Choleraesuis vector delivering SaoA antigen confers protection against Streptococcus suis serotypes 2 and 7 in mice and pigs.

    Science.gov (United States)

    Li, Yu-An; Ji, Zhenying; Wang, Xiaobo; Wang, Shifeng; Shi, Huoying

    2017-12-21

    Streptococcus suis is one of the major pathogens that cause economic losses in the swine industry worldwide. However, current bacterins only provide limited prophylactic protection in the field. An ideal vaccine against S. suis should protect pigs against the clinical diseases caused by multiple serotypes, or at least protect against the dominant serotype in a given geographic region. A new recombinant Salmonella enterica serotype Choleraesuis vaccine vector, rSC0011, that is based on the regulated delayed attenuation system and regulated delayed antigen synthesis system, was developed recently. In this study, an improved recombinant attenuated Salmonella Choleraesuis vector, rSC0016, was developed by incorporating a sopB mutation to ensure adequate safety and maximal immunogenicity. In the spleens of mice, rSC0016 colonized less than rSC0011. rSC0016 and rSC0011 colonized similarly in Peyer's patches of mice. The recombinant vaccine rSC0016(pS-SaoA) induced stronger cellular, humoral, and mucosal immune responses in mice and swine against SaoA, a conserved surface protein that is present in many S. suis serotypes, than did rSC0011(pS-SaoA) without sopB or rSC0018(pS-SaoA), which is an avirulent, chemically attenuated vaccine strain. rSC0016(pS-SaoA) provided 100% protection against S. suis serotype 2 in mice and pigs, and full cross-protection against SS7 in pigs. This new vaccine vector provides a foundation for the development of a universal vaccine against multiple serotypes of S. suis in pigs.

  17. The transcriptome of Trichuris suis--first molecular insights into a parasite with curative properties for key immune diseases of humans.

    Directory of Open Access Journals (Sweden)

    Cinzia Cantacessi

    Full Text Available Iatrogenic infection of humans with Trichuris suis (a parasitic nematode of swine is being evaluated or promoted as a biological, curative treatment of immune diseases, such as inflammatory bowel disease (IBD and ulcerative colitis, in humans. Although it is understood that short-term T. suis infection in people with such diseases usually induces a modified Th2-immune response, nothing is known about the molecules in the parasite that induce this response.As a first step toward filling the gaps in our knowledge of the molecular biology of T. suis, we characterised the transcriptome of the adult stage of this nematode employing next-generation sequencing and bioinformatic techniques. A total of ∼65,000,000 reads were generated and assembled into ∼20,000 contiguous sequences ( = contigs; ∼17,000 peptides were predicted and classified based on homology searches, protein motifs and gene ontology and biological pathway mapping.These analyses provided interesting insights into a number of molecular groups, particularly predicted excreted/secreted molecules (n = 1,288, likely to be involved in the parasite-host interactions, and also various molecules (n = 120 linked to chemokine, T-cell receptor and TGF-β signalling as well as leukocyte transendothelial migration and natural killer cell-mediated cytotoxicity, which are likely to be immuno-regulatory or -modulatory in the infected host. This information provides a conceptual framework within which to test the immunobiological basis for the curative effect of T. suis infection in humans against some immune diseases. Importantly, the T. suis transcriptome characterised herein provides a curated resource for detailed studies of the immuno-molecular biology of this parasite, and will underpin future genomic and proteomic explorations.

  18. Use of a Mycoplasma suis-PCR protocol for screening a population of captive peccaries (Tayassu tajacu and Tayassu pecari Uso de um protocolo de PCR para a detecção de Mycoplasma suis para avaliação de uma população de catetos e queixadas de cativeiro (Tayassu tajacu and Tayassu pecari

    Directory of Open Access Journals (Sweden)

    Rafael Felipe da Costa Vieira

    2011-03-01

    Full Text Available Mycoplasma suis is a hemotropic bacteria of red blood cells and the causative agent of swine eperythrozoonosis. Diagnosis of infection may be reached by direct examination of blood smears; however, the use of polymerase chain reaction (PCR of the 16S RNA gene of M. suis improves the sensitivity and specificity of detection. The aim of this study was to screen peccaries (Tayassu tajacu and T. pecari for M. suis infection using a specific conventional PCR. A total of 28 blood samples from captive collared and white-lipped peccaries were collected, DNA extracted and a specific M. suis PCR assay performed. All samples were negatives by both blood smear examination and PCR testing. To verify the presence of amplifiable DNA, PCR for beta-actin gene was performed in all samples. This study was part of an active surveillance program, which is crucial for monitoring animal health status, particularly in wildlife species.Mycoplasma suis é uma bactéria hemotrópica dos eritrócitos e é o agente causador da eperitrozoonose suína. O diagnóstico da infecção pode ser realizado pelo exame direto de esfregaços sanguíneos; entretanto, o uso da reação em cadeia da polimerase (PCR baseada no gene 16S RNA de M. suis aumenta a sensibilidade e especificidade da detecção. O objetivo deste estudo foi avaliar catetos e queixadas (Tayassu tajacu e T. pecari para a infecção por M. suis, utilizando PCR convencional específico. Um total de 28 amostras de sangue de catetos e queixadas de cativeiro foram coletadas, o DNA foi extraído e a PCR específica para a detecção de M. suis realizada. Todas as amostras foram negativas pelo esfregaço sanguíneo e PCR. Para verificar a presença de DNA amplificável, PCR para o gene da beta actina foi realizada em todas as amostras. Este estudo foi parte de um programa de vigilância ativa, o qual é crucial para o monitoramento do estado de saúde animal, particularmente em espécies selvagens.

  19. [Identification and detection of trag: a new infection-related gene expressed in vivo from isolates of Streptococcus suis].

    Science.gov (United States)

    Zhu, Haodan; Gu, Hongwei; Lu, Chengping

    2008-12-01

    The trag (transfer gene G) was one of the novel infection-related factors identified by in vivo-induced antigen technology (IVIAT) from Streptococcus suis type 2 expression libraries with swine convalesecent sera in our former research. We detected the distribution of trag in different Streptococcus suis isolates and identify the differential expression of the new infection-related factor between in vivo and in vitro condition. According to the sequence of trag of North American strain 89/1591, a pair of primers were designed to detect the distribution of trag in total 43 SS isolates. Another pair of primers were designed to amplify the ORF of trag of 5 SS representive strains (ZY05719, HA9801, 98012, SH040805, SH040917). Partial gene of trag was cloned and inserted into expression vector pET28a(+), and induced by IPTG to express recombinant TRAG. The recombinant protein was probed with swine convalescent sera and immune sera respectively. The trag was detected in the most of SS2 isolates (30/32), in SS9 isolates (4/6), and 1 isolate of SS7, while it was not found in SS2 European strain ATCC43765, avirulent strain SS2 T15, 1 isolates of SS1, 1 isolates of SS1/2 and 2 isolates of group C streptococcal strains from pigs. Comparisons between the sequences of TRAG of 5 isolates with that of SS isolates, showed a high homology (>97%) with North American strain 89/1589 and China strains 98HAH33, 05ZYH33. The immunoreactivity was only presented with convalescent sera. The trag was detected from virulent SS isolates but not from avirulent strain, which suggested that this gene may be related to the pathogenicity of SS. The special reactivity was only present with convalescent sera, and it indicated that TRAG might play a role during SS2 invasive course.

  20. A data bank about wild rodent specimens of Italy / Una banca dati sui roditori selvatici d'Italia

    Directory of Open Access Journals (Sweden)

    Giovanni Amori

    1987-07-01

    Full Text Available Abstract The setting up of a "data bank" dealing with the findings of Rodents specimens (Families: Gliridae, Arvicolidae, Muridae of peninsular and insular Italy available for 1950-1981 years in the italian museums and collections. The file, wherever possible, has been filled up with cartographic, bioclimatic and synanthropic data. With the use of a microcomputer has thus become possible to make inquires related both to biological and environmental data. Data management allows the following function: file maintenance (insert, delete and modify record; cross - reference search; search index; report generation. In the first phase challenges have been formulated, requiring simple data processing. Some informations were obtained as reply about the prevalences in the capturing referred to sex and season, as well as the connections between Rodents coenosis and bioclimatic condition of the territory. Riassunto È stata avviata una "banca dati" dei rinvenimenti di esemplari di Roditori (Famiglie: Gliridae, Arvicolidae, Muridae dell'Italia peninsulare ed insulare catalogati negli anni 1950-1981 nei Musei e collezioni italiani. L'archivio, laddove possibile, è stato completato con i dati cartografici, bioclimatici e sinantropici. L'uso del microcomputer ha permesso di formulare domande incrociate sia sui dati biologici che su quelli ambientali. La gestione dei dati consente le seguenti funzioni: gestione dell'archivio (inserimento, cancellazione e modifica del record; ricerca incrociata; ricerca per indice; stampa. In una prima fase sono state formulate alcune domande richiedenti semplici elaborazioni sulle preponderanze delle catture in rapporto al sesso ed alla stagione, nonché sui rapporti tra cenosi a Roditori ed assetto bioclimatico del territorio.

  1. Construction, characterization and evaluation of the protective efficacy of the Streptococcus suis double mutant strain ΔSsPep/ΔSsPspC as a live vaccine candidate in mice.

    Science.gov (United States)

    Hu, Jin; You, Wujin; Wang, Bin; Hu, Xueying; Tan, Chen; Liu, Jinlin; Chen, Huanchun; Bei, Weicheng

    2015-01-01

    Streptococcus suis serotype 2 (S. suis 2) causes sepsis and meningitis in piglets and humans, and results in one of the most serious bacterial diseases affecting the production of commercial pigs around the world. Due to the failure of the current inactivated vaccine to protect against the disease, development of a new attenuated live vaccine against S. suis 2 by deleting essential virulence factors is urgently needed. We have previously reported the construction and characterization of an SsPep single gene deletion mutant strain ΔSsPep based on S. suis 2. Our previous results have shown that SsPep plays a critical role in the pathogenesis of S. suis 2. In this study, a precisely defined double-deletion mutant ΔSsPep/ΔSsPspC of S. suis 2 without antibiotic-resistance markers was constructed based on ΔSsPep, and the levels of virulence of the wild-type (WT) and ΔSsPep/ΔSsPspC were compared in a mouse experimental infection model. We demonstrated that the double mutant ΔSsPep/ΔSsPspC was less virulent than the WT, and could induce a noticeable antibody response. Analysis of IgG subclasses (IgG1 and IgG2a) indicated that both Th1 and Th2 responses were induced by ΔSsPep/ΔSsPspC, although the IgG2a (Th1) response predominated over the IgG1 (Th2) response. Moreover, ΔSsPep/ΔSsPspC could confer 90% protective efficacy against challenge with a lethal dose of fully virulent S. suis 2. Taken together, these data demonstrate that ΔSsPep/ΔSsPspC can be used as an effective live vaccine and provide a novel strategy against infection of S. suis 2. Copyright © 2014 Elsevier GmbH. All rights reserved.

  2. Concentração inibitória mínima (CIM de oito antimicrobianos frente isolados de Streptococcus suis

    Directory of Open Access Journals (Sweden)

    Felipe Masiero Salvarani

    2008-02-01

    Full Text Available The minimun inhibitory concentration (MIC was determined toward amoxicilin, ampicilin, penicillin, ceftiofur, florfenicol, lincomycin, trimethoprim-sulfadiazine and tetracycline for 75 strains of Streptococcus suis. The MIC was performed on sheep blood agar plates containing concentrations varying from 0,25 to 256 µg/ml of the antibiotic described above. The amoxicilin in the concentrations of 1 and 2 µg/ml, florfenicol in the concentration of 1 µg/ml and trimethoprim-sulfadiazine in the concentrations of 2 e 8 µg/ml, were the antibiotics that presented minor resistance. In contrast, the ampicilin, tetracycline, ceftiofur and lincomycin, presented MIC of 64 and 128 µg/ml, 64 and 128 µg/ml, 128 and 256 µg/ml and >;256 µg/ml, respectively. The results of this study show that the amoxicilin and florfenicol are the antibiotics of choice for the treatment of diseases by S. suis in swine.

  3. Patterns of antimicrobial resistance in Streptococcus suis isolates from pigs with or without streptococcal disease in England between 2009 and 2014.

    Science.gov (United States)

    Hernandez-Garcia, Juan; Wang, Jinhong; Restif, Olivier; Holmes, Mark A; Mather, Alison E; Weinert, Lucy A; Wileman, Thomas M; Thomson, Jill R; Langford, Paul R; Wren, Brendan W; Rycroft, Andrew; Maskell, Duncan J; Tucker, Alexander W

    2017-08-01

    Antimicrobial resistance in Streptococcus suis, a global zoonotic pathogen of pigs, has been mostly studied only in diseased animals using surveys that have not evaluated changes over time. We compared patterns of resistance between S. suis isolates from clinical cases of disease (CC) and non-clinical case (NCC) pigs in England, collected over two discrete periods, 2009-2011 and 2013-2014. Minimum inhibitory concentrations (MIC) of 17 antimicrobials (nine classes) were determined on 405 S. suis isolates categorised by sampling period and disease association to assess changes in resistance over time and association with disease. First, isolates were characterized as resistant or susceptible using published clinical breakpoints. Second, epidemiological cut-offs (ECOFF) were derived from MIC values, and isolates classified as wild type (WT) below the ECOFF and non-wild type (NWT) above the ECOFF. Finally, isolate subsets were analysed for shifts in MIC distribution. NCC isolates were more resistant than CC isolates to cephalosporins, penams, pleuromutilins, potentiated sulphonamides and tetracyclines in both study periods. Resistance levels among CC isolates increased in 2013-2014 relative to 2009-2011 for antimicrobials including aminoglycosides, cephalosporins, fluoroquinolones, pleuromutilins, potentiated sulphonamides and tetracyclines. The prevalence of isolates categorised as NWT for five or more classes of antimicrobials was greater among NCC than CC isolates for both time periods, and increased with time. This study used standardised methods to identify significant shifts in antimicrobial resistance phenotypes of S. suis isolated from pigs in England, not only over time but also between isolates from known clinical cases or disease-free pigs. Copyright © 2017. Published by Elsevier B.V.

  4. New view on the age-specificity of pig Cryptosporidium by species-specific primers for distinguishing Cryptosporidium suis and Cryptosporidium pig genotype II

    Czech Academy of Sciences Publication Activity Database

    Jeníková, M.; Němejc, K.; Sak, Bohumil; Květoňová, Dana; Kváč, Martin

    2011-01-01

    Roč. 176, 2/3 (2011), 120-125 ISSN 0304-4017 R&D Projects: GA ČR GP523/07/P117 Institutional research plan: CEZ:AV0Z60220518 Keywords : Cryptosporidium suis * Cryptosporidium pig genotype II * Mixed infection * Age-specificity * Species-specific primers Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine Impact factor: 2.579, year: 2011

  5. PREPARATIVE ISOLATION AND PURIFICATION OF THREE GLYCINE-CONJUGATED CHOLIC ACIDS FROM PULVIS FELLIS SUIS BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY COUPLED WITH ELSD DETECTION

    OpenAIRE

    He, Jiao; Li, Jing; Sun, Wenji; Zhang, Tianyou; Ito, Yoichiro

    2012-01-01

    Coupled with evaporative light scattering detection, a high-speed counter-current chromatography (HSCCC) method was developed for preparative isolation and purification of three glycine-conjugated cholic acids, glycochenodeoxycholic acid (GCDCA), glycohyodeoxycholic acid (GHDCA) and glycohyocholic acid (GHCA) from Pulvis Fellis Suis (Pig gallbladder bile) for the first time. The separation was performed with a two-phase solvent system consisted of chloroform-methanol-water-acetic acid (65:30:...

  6. Long-term clinical outcomes with the retropubic tension-free vaginal tape (TVT) procedure compared to Burch colposuspension for correcting stress urinary incontinence (SUI).

    Science.gov (United States)

    Holdø, Bjørn; Verelst, Margareta; Svenningsen, Rune; Milsom, Ian; Skjeldestad, Finn Egil

    2017-11-01

    The retropubic tension-free vaginal tape (TVT) procedure replaced Burch colposuspension as the primary surgical method for stress urinary incontinence (SUI) and mixed urinary incontinence (MUI) in women in our department in 1998. In this study we compared the short-term and long-term clinical outcomes of these surgical procedures. Using a case series design, we compared the last 5 years of the Burch procedure (n = 127, 1994-1999) with the first 5 years of the retropubic TVT procedure (n = 180, 1998-2002). Information from the medical records was transferred to a case report form comprising data on perioperative and long-term complications as well as recurrence of UI, defined as bothersome UI or UI in need of repeat surgery. Other endpoints were rates of perioperative and late complications and the rates of prolapse surgery after primary surgery. The data were analyzed with the chi-squared and t tests and survival analysis using SPSS. The cumulative recurrence rate of SUI in women with preoperative SUI was significantly higher after the Burch procedure, but no difference was observed in women with MUI. There were no significant differences in rates of perioperative and late complications. At 12 years there was a significant increase in rates of repeat surgery for incontinence and prolapse in women after the Burch procedure. The long-term efficacy of TVT surgery was superior to that of Burch colposuspension in women with SUI. In addition, the rate of late prolapse surgery was significantly higher after the Burch procedure.

  7. First insights into the protective effects of a recombinant swinepox virus expressing truncated MRP of Streptococcus suis type 2 in mice.

    Science.gov (United States)

    Huang, Dongyan; Zhu, Haodan; Lin, Huixing; Xu, Jiarong; Lu, Chengping

    2012-01-01

    To explore the potential of the swinepox virus (SPV) as vector for Streptococcus suis vaccines, a vector system was developed for the construction of a recombinant SPV carrying bacterial genes. Using this system, a recombinant virus expressing truncated muramidase-released protein (MRP) of S. suis type 2 (SS2), designated rSPV-MRP, was produced and identified by PCR, western blotting and immunofluorescence assays. The rSPV-MRP was found to be only slightly attenuated in PK-15 cells, when compared with the wild-type virus. After immunization intramuscularly with rSPV-MRP, SS2 inactive vaccine (positive control), wild-type SPV (negative control) and PBS (blank control) respectively, all CD1 mice were challenged with a lethal dose or a sublethal dose of SS2 highly virulent strain ZY05719. While SS2 inactive vaccine protected all mice, immunization with rSPV-MRP resulted in 60% survival and protected mice against a lethal dose of the highly virulent SS2 strain, compared with the negative control (P MRP had a significantly reduced bacterial burden in all organs examined, compared to negative controls and blank controls (P MRP-vaccinated group were significantly higher (P MRP provided mice with protection from systemic SS2 infection. If SPV recombinants have the potential as S. suis vaccines for the use in pigs has to be evaluated in further studies.

  8. A Hashtag Worth a Thousand Words: Discursive Strategies Around #JeNeSuisPasCharlie After the 2015 Charlie Hebdo Shooting

    Directory of Open Access Journals (Sweden)

    Fabio Giglietto

    2017-01-01

    Full Text Available Following a shooting attack by two self-proclaimed Islamist gunmen at the offices of French satirical weekly Charlie Hebdo on 7 January 2015, there emerged the hashtag #JeSuisCharlie on Twitter as an expression of solidarity and support for the magazine’s right to free speech. Almost simultaneously, however, there was also #JeNeSuisPasCharlie explicitly countering the former, affirmative hashtag. Based on a multimethod analysis of 74,047 tweets containing #JeNeSuisPasCharlie posted between 7 and 11 January, this article reveals that users of the hashtag under study employed various discursive strategies and tactics to challenge the mainstream framing of the shooting as the universal value of freedom of expression being threatened by religious extremism, while protecting themselves from the risk of being viewed as disrespecting victims or endorsing the violence committed. The significance of this study is twofold. First, it extends the literature on strategic speech acts by examining how such acts take place in a social media context. Second, it highlights the need for a multidimensional and reflective methodology when dealing with data mined from social media.

  9. Serological patterns of Actinobacillus pleuropneumoniae, Mycoplasma hyopneumoniae, Pasteurella multocida and Streptococcus suis in pig herds affected by pleuritis.

    Science.gov (United States)

    Wallgren, Per; Nörregård, Erik; Molander, Benedicta; Persson, Maria; Ehlorsson, Carl-Johan

    2016-10-04

    Respiratory illness is traditionally regarded as the disease of the growing pig, and has historically mainly been associated to bacterial infections with focus on Mycoplasma hyopneumoniae and Actinobacillus pleuropneumoniae. These bacteria still are of great importance, but continuously increasing herd sizes have complicated the scenario and the influence of secondary invaders may have been increased. The aim of this study was to evaluate the presence of A. pleuropneumoniae and M. hyopneumoniae, as well as that of the secondary invaders Pasteurella multocida and Streptococcus suis by serology in four pig herds (A-D) using age segregated rearing systems with high incidences of pleuritic lesions at slaughter. Pleuritic lesions registered at slaughter ranged from 20.5 to 33.1 % in the four herds. In herd A, the levels of serum antibodies to A. pleuropneumoniae exceeded A 450  > 1.5, but not to any other microbe searched for. The seroconversion took place early during the fattening period. Similar levels of serum antibodies to A. pleuropneumoniae were also recorded in herd B, with a subsequent increase in levels of antibodies to P. multocida. Pigs seroconverted to both agents during the early phase of the fattening period. In herd C, pigs seroconverted to P. multocida during the early phase of the fattening period and thereafter to A. pleuropneumoniae. In herd D, the levels of antibodies to P. multocida exceeded A 450  > 1.0 in absence (A 450  hyopneumoniae and to S. suis remained below A 450  hyopneumoniae late during the rearing period (herd B-D), or not at all (herd A). Different serological patterns were found in the four herds with high levels of serum antibodies to A. pleuropneumoniae and P. multocida, either alone or in combination with each other. Seroconversion to M. hyopneumoniae late during the rearing period or not at all, confirmed the positive effect of age segregated rearing in preventing or delaying infections with M

  10. RegA Plays a Key Role in Oxygen-Dependent Establishment of Persistence and in Isocitrate Lyase Activity, a Critical Determinant of In vivo Brucella suis Pathogenicity

    Directory of Open Access Journals (Sweden)

    Elias Abdou

    2017-05-01

    Full Text Available For aerobic human pathogens, adaptation to hypoxia is a critical factor for the establishment of persistent infections, as oxygen availability is low inside the host. The two-component system RegB/A of Brucella suis plays a central role in the control of respiratory systems adapted to oxygen deficiency, and in persistence in vivo. Using an original “in vitro model of persistence” consisting in gradual oxygen depletion, we compared transcriptomes and proteomes of wild-type and ΔregA strains to identify the RegA-regulon potentially involved in the set-up of persistence. Consecutive to oxygen consumption resulting in growth arrest, 12% of the genes in B. suis were potentially controlled directly or indirectly by RegA, among which numerous transcriptional regulators were up-regulated. In contrast, genes or proteins involved in envelope biogenesis and in cellular division were repressed, suggesting a possible role for RegA in the set-up of a non-proliferative persistence state. Importantly, the greatest number of the RegA-repressed genes and proteins, including aceA encoding the functional IsoCitrate Lyase (ICL, were involved in energy production. A potential consequence of this RegA impact may be the slowing-down of the central metabolism as B. suis progressively enters into persistence. Moreover, ICL is an essential determinant of pathogenesis and long-term interactions with the host, as demonstrated by the strict dependence of B. suis on ICL activity for multiplication and persistence during in vivo infection. RegA regulates gene or protein expression of all functional groups, which is why RegA is a key regulator of B. suis in adaptation to oxygen depletion. This function may contribute to the constraint of bacterial growth, typical of chronic infection. Oxygen-dependent activation of two-component systems that control persistence regulons, shared by several aerobic human pathogens, has not been studied in Brucella sp. before. This work

  11. Effect of Early-Life Treatment of Piglets with Long-Acting Ceftiofur on Colonization of Streptococcus suis Serotype 7 and Elicitation of Specific Humoral Immunity in a Farm Dealing with Streptococcal Diseases

    Directory of Open Access Journals (Sweden)

    Christine Unterweger

    2018-03-01

    Full Text Available In newborn piglets treatment with long-acting ceftiofur is a common approach to reduce losses due to streptococcal diseases on farms, even if problems start after weaning. The purpose of this study was to examine the influence of a single early-life treatment on Streptococcus (S. suis colonization, transmission, immunoreaction, and drug resistance over an observation period of 14 weeks. In a farm with a history of streptococcal disease and isolation of a S. suis cps 7 mrp+, arcA+ isolate from diseased piglets, half of each litter was treated with a long-acting ceftiofur on day 1. S. suis-isolates were profiled and serum samples were tested for opsonizing antibodies. Treated and untreated pigs did not differ according to average daily weight gains, S. suis-isolation rates and level of opsonizing antibodies. Although the invasive cps 7 strain was not detected in a single piglet over 14 weeks, all animals developed bactericidal activity. No resistance to ceftiofur, but resistance to tetracyclins (100%, and trimethoprim/sulfamethoxazole (53% was shown. Our results indicate that early treatment with ceftiofur does not prevent colonization and transmission of S. suis or the induction of bactericidal humoral immunity in nursery and fattening pigs. The necessity of continuous usage should be reconsidered.

  12. Sorotipagem de amostras de Streptococcus suis isoladas de suínos em granjas dos Estados de São Paulo, Minas Gerais e Paraná Serotyping of Streptococcus suis strains isolated from pigs in the States of São Paulo, Minas Gerais e Paraná, Brazil

    Directory of Open Access Journals (Sweden)

    Keila J.R. Pagnani

    2002-01-01

    Full Text Available Infecções causadas por Streptococcus suis são muito comuns em países onde a indústria de carne suína é desenvolvida. Estas infecções estão relacionadas a casos clínicos de broncopneumonia, meningite, artrite, pericardite, miocardite, endocardite, poliserosite fibrinosa, septicemia, rinite e aborto. Esta bactéria também foi descrita como patógeno de ruminantes e humanos. No Brasil há evidências clínicas da existência de processos infecciosos causados por S. suis afetando mais de 50% das granjas em Estados como São Paulo, Minas Gerais e Paraná. No presente estudo foram isoladas 51 amostras de S. suis de granjas do Estados acima referidos, coletadas de diferentes casos clínicos como septicemia, meningite, artrite e pneumonia, tendo sido obtidas ou em cultura pura ou como patógeno de maior predominância nos tecidos de suínos. Este material foi semeado em Columbia ágar sangue adicionado de 5% de sangue bovino e incubado a 37°C por 24 horas. Para a identificação bioquímica as colônias que apresentavam a-hemólise, bem como as amostras padrão, foram submetidas a testes convencionais para a confirmação da espécie S. suis, tais como: hidrólise de arginina, teste de Voges-Proskauer, e produção de ácido a partir de vários carboidratos (inulina, salicina, trealose, lactose, sacarose, sorbitol, manitol e glicerol. As amostras também foram testadas para habilidade de crescimento em meio de TSA com 6,5% de NaCl e para a produção de amilase. Todas as amostras que fizeram parte desta pesquisa foram testadas pelo sistema Api 20 Strep para confirmação dos resultados obtidos nos testes convencionais. Para a sorotipagem foram produzidos antissoros de 1 a 8. Outras amostras não pertencentes a estes sorotipos também foram sorotipadas. O antissoro produzido em coelhos foi titulado pelo teste de aglutinação em tubo com 2-mercaptoetanol e pelo teste de reação capsular e, quando adequados, foram usados no teste de co

  13. HP1330 Contributes to Streptococcus suis Virulence by Inducing Toll-Like Receptor 2- and ERK1/2-Dependent Pro-inflammatory Responses and Influencing In Vivo S. suis Loads

    Directory of Open Access Journals (Sweden)

    Qiang Zhang

    2017-07-01

    Full Text Available Streptococcus suis 2 (SS2 has evolved into a highly invasive pathogen responsible for two large-scale outbreaks of streptococcal toxic shock-like syndrome (STSLS in China. Excessive inflammation stimulated by SS2 is considered a hallmark of STSLS, even it also plays important roles in other clinical symptoms of SS2-related disease, including meningitis, septicemia, and sudden death. However, the mechanism of SS2-caused excessive inflammation remains poorly understood. Here, a novel pro-inflammatory protein was identified (HP1330, which could induce robust expression of pro-inflammatory cytokines (TNF-α, MCP-1, and IL-1β in RAW264.7 macrophages. To evaluate the role of HP1330 in SS2 virulence, an hp1330-deletion mutant (Δhp1330 was constructed. In vitro, hp1330 disruption led to a decreased pro-inflammatory ability of SS2 in RAW 264.7 macrophages. In vivo, Δhp1330 showed reduced lethality, pro-inflammatory activity, and bacterial loads in mice. To further elucidate the mechanism of HP1330-induced pro-inflammatory cytokine production, antibody blocking and gene-deletion experiments with macrophages were performed. The results revealed that the pro-inflammatory activity of HP1330 depended on the recognition of toll-like receptor 2 (TLR2. Furthermore, a specific inhibitor of the extracellular signal-regulated kinase 1/2 (ERK1/2 pathways could significantly decrease HP1330-induced pro-inflammatory cytokine production, and western blot analysis showed that HP1330 could induce activation of the ERK1/2 pathway. Taken together, our findings demonstrate that HP1330 contributes to SS2 virulence by inducing TLR2- and ERK1/2-dependent pro-inflammatory cytokine production and influencing in vivo bacterial loads, implying that HP1330 may be associated with STSLS caused by SS2.

  14. Fecundidade das mulheres autodeclaradas indígenas residentes em Minas Gerais, Brasil: uma análise a partir do Censo Demográfico 2000 Fertility of indigenous women in Minas Gerais State, Brazil: an analysis using the 2000 census

    Directory of Open Access Journals (Sweden)

    Cláudio Santiago Dias Júnior

    2008-11-01

    Full Text Available As populações indígenas aldeadas no Brasil apresentam alta taxa de fecundidade total (TFT, sendo que em alguns casos é observado o crescimento destas taxas ao longo do tempo. Já os dados do Instituto Brasileiro de Geografia e Estatística (IBGE revelam um declínio da TFT da população autodeclarada indígena, independente da situação de domicílio (urbano, rural e rural específico. Este trabalho utiliza dados do Censo Demográfico 2000 para descrever e comparar a estrutura e o nível da fecundidade de período e coorte das mulheres autodeclaradas indígenas residentes em Minas Gerais, Brasil. Foram calculadas as taxas específicas de fecundidade (TEF, a TFT de período para o ano 2000 e a TFT de coorte para mulheres com cinqüenta anos e mais de idade. Os resultados mostram que as mulheres autodeclaradas indígenas residentes em Minas Gerais experimentaram um passado de alta fecundidade, independente da situação de domicílio. Concluiu-se que o uso do Censo Demográfico 2000 é uma importante fonte de dados para os estudos sobre populações indígenas no Brasil, tanto pelo seu alto grau de cobertura quanto pela riqueza das informações coletadas.Indigenous populations living in villages in Brazil have presented high total fertility rates (TFR that have increased over time in some cases. Meanwhile, data from the Brazilian Institute of Geography and Statistics (IBGE or National Census Bureau show a decline in the TFR for the total self-declared indigenous population (combining urban, rural, and specific rural residence. The current study uses data from the 2000 Population Census to describe and compare the structure and level of period fertility for the cohort of self-declared indigenous women living in the State of Minas Gerais. We calculated age-specific fertility rates (ASFR, the period TFR for 2000, and the cohort TFR for women 50 years and older. According to the findings, self-declared indigenous women living in Minas Gerais

  15. Detección de Mycoplasma suis en casos clínicos de síndrome del desmedro multisistémico posdestete en porcinos Detection of Mycoplasma suis in clinical cases with postweaning multisystemic wasting syndrome in swine

    Directory of Open Access Journals (Sweden)

    N. B. Pereyra

    2006-09-01

    Full Text Available Mycoplasma suis es un parásito obligado de los eritrocitos del cerdo. Produce anemia aguda o crónica y afecta a distintas categorías de animales. El síndrome del desmedro multisistémico posdestete (PMWS se considera causado por el circovirus porcino tipo 2 (PCV-2, aunque muchos aspectos de la patogenia del síndrome permanecen sin aclarar. Se manifiesta a través de retraso del crecimiento, anemia e ictericia en cerdos de 5 a 12 semanas de edad, en los que se produce una inmunosupresión que deriva en coinfecciones bacterianas. Se estudiaron tres granjas porcinas con sintomatología asociada a la presencia de estos dos agentes etiológicos. Se observaron formas compatibles con M. suis en extendidos de sangre y en lesiones histopatológicas indicativas de PMWS, en cortes de tejidos. Esta es la primera comunicación acerca de la asociación clínica entre las dos entidades mencionadas.Mycoplasma suis is a swine erythrocyte obligatory parasite. Its presence may result in chronic or acute anaemia in different pig categories. It is considered that the postweaning multisystemic wasting syndrome (PMWS is caused by porcine circovirus type 2, but some aspects of the pathogenesis remain unknown. PMWS signs are impaired weight gain, anaemia and jaundice in 5 to 12 week-old pigs that suffer from immunosuppression and bacterial co-infections. The pigs with signs of these diseases on three porcine farms were studied. Compatible M. suis forms in blood smears and typical PMWS lesions in tissue cuts were seen. This is the first communication of the clinical association between these two entities.

  16. The non-conserved region of MRP is involved in the virulence of Streptococcus suis serotype 2.

    Science.gov (United States)

    Li, Quan; Fu, Yang; Ma, Caifeng; He, Yanan; Yu, Yanfei; Du, Dechao; Yao, Huochun; Lu, Chengping; Zhang, Wei

    2017-10-03

    Muramidase-released protein (MRP) of Streptococcus suis serotype 2 (SS2) is an important epidemic virulence marker with an unclear role in bacterial infection. To investigate the biologic functions of MRP, 3 mutants named Δmrp, Δmrp domain 1 (Δmrp-d1), and Δmrp domain 2 (Δmrp-d2) were constructed to assess the phenotypic changes between the parental strain and the mutant strains. The results indicated that MRP domain 1 (MRP-D1, the non-conserved region of MRP from a virulent strain, a.a. 242-596) played a critical role in adherence of SS2 to host cells, compared with MRP domain 1* (MRP-D1*, the non-conserved region of MRP from a low virulent strain, a.a. 239-598) or MRP domain 2 (MRP-D2, the conserved region of MRP, a.a. 848-1222). We found that MRP-D1 but not MRP-D2, could bind specifically to fibronectin (FN), factor H (FH), fibrinogen (FG), and immunoglobulin G (IgG). Additionally, we confirmed that mrp-d1 mutation significantly inhibited bacteremia and brain invasion in a mouse infection model. The mrp-d1 mutation also attenuated the intracellular survival of SS2 in RAW246.7 macrophages, shortened the growth ability in pig blood and decreased the virulence of SS2 in BALB/c mice. Furthermore, antiserum against MRP-D1 was found to dramatically impede SS2 survival in pig blood. Finally, immunization with recombinant MRP-D1 efficiently enhanced murine viability after SS2 challenge, indicating its potential use in vaccination strategies. Collectively, these results indicated that MRP-D1 is involved in SS2 virulence and eloquently demonstrate the function of MRP in pathogenesis of infection.

  17. Differentiation of highly virulent strains of Streptococcus suis serotype 2 according to glutamate dehydrogenase electrophoretic and sequence type.

    Science.gov (United States)

    Kutz, Russell; Okwumabua, Ogi

    2008-10-01

    The glutamate dehydrogenase (GDH) enzymes of 19 Streptococcus suis serotype 2 strains, consisting of 18 swine isolates and 1 human clinical isolate from a geographically varied collection, were analyzed by activity staining on a nondenaturing gel. All seven (100%) of the highly virulent strains tested produced an electrophoretic type (ET) distinct from those of moderately virulent and nonvirulent strains. By PCR and nucleotide sequence determination, the gdh genes of the 19 strains and of 2 highly virulent strains involved in recent Chinese outbreaks yielded a 1,820-bp fragment containing an open reading frame of 1,344 nucleotides, which encodes a protein of 448 amino acid residues with a calculated molecular mass of approximately 49 kDa. The nucleotide sequences contained base pair differences, but most were silent. Cluster analysis of the deduced amino acid sequences separated the isolates into three groups. Group I (ETI) consisted of the seven highly virulent isolates and the two Chinese outbreak strains, containing Ala(299)-to-Ser, Glu(305)-to-Lys, and Glu(330)-to-Lys amino acid substitutions compared with groups II and III (ETII). Groups II and III consisted of moderately virulent and nonvirulent strains, which are separated from each other by Tyr(72)-to-Asp and Thr(296)-to-Ala substitutions. Gene exchange studies resulted in the change of ETI to ETII and vice versa. A spectrophotometric activity assay for GDH did not show significant differences between the groups. These results suggest that the GDH ETs and sequence types may serve as useful markers in predicting the pathogenic behavior of strains of this serotype and that the molecular basis for the observed differences in the ETs was amino acid substitutions and not deletion, insertion, or processing uniqueness.

  18. [Serological detection of Brucella suis, influenza virus and Aujeszky's disease virus in backyard and small swine holders in Argentina].

    Science.gov (United States)

    Dibarbora, Marina; Cappuccio, Javier A; Aznar, María N; Bessone, Fernando A; Piscitelli, Hernán; Pereda, Ariel J; Pérez, Daniel R

    Farmers raising less than 100 sows represent more than 99% of swine producers in Argentina, although little is known about their sanitary status and productive characteristics in the country. Sanitary and productive information was obtained. Furthermore, samples for serological studies were taken to detect antibodies against Brucella suis (Bs), Aujeszky's disease virus (AV) and influenza virus (IV) in 68 backyard and small producers with less than 100 sows located in the north, central and south regions of Argentina. Antibodies against H1 pandemic were detected in 80% of the farms while 11%, 11.7% and 6.0% of the producers were positive to influenza H3 cluster 2, AV and Bs, respectively. None of the producers was aware of the risk factors concerning the transmission of diseases from pigs to humans. A percentage of 47% of them buy pigs for breeding from other farmers and markets. With regard to biosecurity measures, only 16% of the farms had perimeter fences. The results of this study demonstrate that productive characterization and disease surveys are important to improve productivity and to reduce the risk of disease transmission among animals and humans. The study of sanitary status and risk factors is necessary for better control and eradication of diseases in backyard or small producers. More representative studies at country level should be carried out to detect the pathogensthat circulate and, with this knowledge, to implement prevention and control measures. Copyright © 2017 Asociación Argentina de Microbiología. Publicado por Elsevier España, S.L.U. All rights reserved.

  19. Trichuris suis and Oesophagostomum dentatum show different sensitivity and accumulation of fenbendazole, albendazole and levamisole in vitro

    DEFF Research Database (Denmark)

    Hansen, Tina Vicky Alstrup; Nejsum, Peter; Friis, Christian

    2014-01-01

    BACKGROUND: The single-dose benzimidazoles used against Trichuris trichiura infections in humans are not satisfactory. Likewise, the benzimidazole, fenbendazole, has varied efficacy against Trichuris suis whereas Oesophagostomum dentatum is highly sensitive to the drug. The reasons for low...... treatment efficacy of Trichuris spp. infections are not known. METHODOLOGY: We studied the effect of fenbendazole, albendazole and levamisole on the motility of T. suis and O. dentatum and measured concentrations of the parent drug compounds and metabolites of the benzimidazoles within worms in vitro....... The motility and concentrations of drug compounds within worms were compared between species and the maximum specific binding capacity (Bmax) of T. suis and O. dentatum towards the benzimidazoles was estimated. Comparisons of drug uptake in living and killed worms were made for both species. PRINCIPAL FINDINGS...

  20. Semi-Preparative Isolation and Purification of Three Tauro-Conjugated Cholic Acids from Pulvis Fellis Suis by HSCCC Coupled with ELSD Detection

    OpenAIRE

    He, Jiao; Zhang, Yongmin; Ito, Yoichiro; Sun, Wenji

    2011-01-01

    Coupled with evaporative light scattering detection, a high-speed counter-current chromatography (HSCCC) method was applied to the separation and purification of three tauro-conjugated cholic acids of taurochenodeoxycholic acid (TCDCA), taurohyodeoxycholic acid (THDCA) and taurohyocholic acid (THCA) from Pulvis Fellis Suis (Pig gallbladder bile) for the first time. The two-phase solvent system composed of chloroform-methanol-water-acetic acid (4:4:2:0.3, v/v/v/v) was selected for the one-step...

  1. The Fast-Growing Brucella suis Biovar 5 Depends on Phosphoenolpyruvate Carboxykinase and Pyruvate Phosphate Dikinase but Not on Fbp and GlpX Fructose-1,6-Bisphosphatases or Isocitrate Lyase for Full Virulence in Laboratory Models

    Directory of Open Access Journals (Sweden)

    Amaia Zúñiga-Ripa

    2018-04-01

    Full Text Available Bacteria of the genus Brucella infect a range of vertebrates causing a worldwide extended zoonosis. The best-characterized brucellae infect domestic livestock, behaving as stealthy facultative intracellular parasites. This stealthiness depends on envelope molecules with reduced pathogen-associated molecular patterns, as revealed by the low lethality and ability to persist in mice of these bacteria. Infected cells are often engorged with brucellae without signs of distress, suggesting that stealthiness could also reflect an adaptation of the parasite metabolism to use local nutrients without harming the cell. To investigate this, we compared key metabolic abilities of Brucella abortus 2308 Wisconsin (2308W, a cattle biovar 1 virulent strain, and B. suis 513, the reference strain of the ancestral biovar 5 found in wild rodents. B. suis 513 used a larger number of C substrates and showed faster growth rates in vitro, two features similar to those of B. microti, a species phylogenomically close to B. suis biovar 5 that infects voles. However, whereas B. microti shows enhanced lethality and reduced persistence in mice, B. suis 513 was similar to B. abortus 2308W in this regard. Mutant analyses showed that B. suis 513 and B. abortus 2308W were similar in that both depend on phosphoenolpyruvate synthesis for virulence but not on the classical gluconeogenic fructose-1,6-bisphosphatases Fbp-GlpX or on isocitrate lyase (AceA. However, B. suis 513 used pyruvate phosphate dikinase (PpdK and phosphoenolpyruvate carboxykinase (PckA for phosphoenolpyruvate synthesis in vitro while B. abortus 2308W used only PpdK. Moreover, whereas PpdK dysfunction causes attenuation of B. abortus 2308W in mice, in B. suis, 513 attenuation occurred only in the double PckA-PpdK mutant. Also contrary to what occurs in B. abortus 2308, a B. suis 513 malic enzyme (Mae mutant was not attenuated, and this independence of Mae and the role of PpdK was confirmed by the lack of

  2. STAR-GENERIS - a software package for information processing

    International Nuclear Information System (INIS)

    Felkel, L.

    1985-01-01

    Man-machine-communication in electrical power plants is increasingly based on the capabilities of minicomputers. Rather than just displaying raw process data more complex processing is done to aid operators by improving information quality. Advanced operator aids for nuclear power plants are, e.g. alarm reduction, disturbance analysis and expert systems. Operator aids use complex combinations and computations of plant signals, which have to be described in a formal and homogeneous way. The design of such computer-based information systems requires extensive software and engineering efforts. The STAR software concept reduces the software effort to a minimum by proving an advanced program package which facilitates specification and implementation of engineering know-how necessary for sophisticated operator aids. (orig./HP) [de

  3. Secondary infection with Streptococcus suis serotype 7 increases the virulence of highly pathogenic porcine reproductive and respiratory syndrome virus in pigs.

    Science.gov (United States)

    Xu, Min; Wang, Shujie; Li, Linxi; Lei, Liancheng; Liu, Yonggang; Shi, Wenda; Wu, Jiabin; Li, Liqin; Rong, Fulong; Xu, Mingming; Sun, Guangli; Xiang, Hua; Cai, Xuehui

    2010-08-09

    Porcine reproductive and respiratory syndrome virus (PRRSV) and Streptococcus suis are common pathogens in pigs. In samples collected during the porcine high fever syndrome (PHFS) outbreak in many parts of China, PRRSV and S. suis serotype 7 (SS7) have always been isolated together. To determine whether PRRSV-SS7 coinfection was the cause of the PHFS outbreak, we evaluated the pathogenicity of PRRSV and/or SS7 in a pig model of single and mixed infection. Respiratory disease, diarrhea, and anorexia were observed in all infected pigs. Signs of central nervous system (CNS) disease were observed in the highly pathogenic PRRSV (HP-PRRSV)-infected pigs (4/12) and the coinfected pigs (8/10); however, the symptoms of the coinfected pigs were clearly more severe than those of the HP-PRRSV-infected pigs. The mortality rate was significantly higher in the coinfected pigs (8/10) than in the HP-PRRSV- (2/12) and SS7-infected pigs (0/10). The deceased pigs of the coinfected group had symptoms typical of PHFS, such as high fever, anorexia, and red coloration of the ears and the body. The isolation rates of HP-PRRSV and SS7 were higher and the lesion severity was greater in the coinfected pigs than in monoinfected pigs. HP-PRRSV infection increased susceptibility to SS7 infection, and coinfection of HP-PRRSV with SS7 significantly increased the pathogenicity of SS7 to pigs.

  4. MLVA genotyping of Brucella melitensis and Brucella abortus isolates from different animal species and humans and identification of Brucella suis vaccine strain S2 from cattle in China.

    Directory of Open Access Journals (Sweden)

    Hai Jiang

    Full Text Available In China, brucellosis is an endemic disease and the main sources of brucellosis in animals and humans are infected sheep, cattle and swine. Brucella melitensis (biovars 1 and 3 is the predominant species, associated with sporadic cases and outbreak in humans. Isolates of B. abortus, primarily biovars 1 and 3, and B. suis biovars 1 and 3 are also associated with sporadic human brucellosis. In this study, the genetic profiles of B. melitensis and B. abortus isolates from humans and animals were analyzed and compared by multi-locus variable-number tandem-repeat analysis (MLVA. Among the B. melitensis isolates, the majority (74/82 belonged to MLVA8 genotype 42, clustering in the 'East Mediterranean' group. Two B. melitensis biovar 1 genotype 47 isolates, belonging to the 'Americas' group, were recovered; both were from the Himalayan blue sheep (Pseudois nayaur, a wild animal. The majority of B. abortus isolates (51/70 were biovar 3, genotype 36. Ten B. suis biovar 1 field isolates, including seven outbreak isolates recovered from a cattle farm in Inner Mongolia, were genetically indistinguishable from the vaccine strain S2, based on MLVA cluster analysis. MLVA analysis provided important information for epidemiological trace-back. To the best of our knowledge, this is the first report to associate Brucella cross-infection with the vaccine strain S2 based on molecular comparison of recovered isolates to the vaccine strain. MLVA typing could be an essential assay to improve brucellosis surveillance and control programs.

  5. Genomic resources and draft assemblies of the human and porcine varieties of scabies mites, Sarcoptes scabiei var. hominis and var. suis.

    Science.gov (United States)

    Mofiz, Ehtesham; Holt, Deborah C; Seemann, Torsten; Currie, Bart J; Fischer, Katja; Papenfuss, Anthony T

    2016-06-02

    The scabies mite, Sarcoptes scabiei, is a parasitic arachnid and cause of the infectious skin disease scabies in humans and mange in other animal species. Scabies infections are a major health problem, particularly in remote Indigenous communities in Australia, where secondary group A streptococcal and Staphylococcus aureus infections of scabies sores are thought to drive the high rate of rheumatic heart disease and chronic kidney disease. We sequenced the genome of two samples of Sarcoptes scabiei var. hominis obtained from unrelated patients with crusted scabies located in different parts of northern Australia using the Illumina HiSeq. We also sequenced samples of Sarcoptes scabiei var. suis from a pig model. Because of the small size of the scabies mite, these data are derived from pools of thousands of mites and are metagenomic, including host and microbiome DNA. We performed cleaning and de novo assembly and present Sarcoptes scabiei var. hominis and var. suis draft reference genomes. We have constructed a preliminary annotation of this reference comprising 13,226 putative coding sequences based on sequence similarity to known proteins. We have developed extensive genomic resources for the scabies mite, including reference genomes and a preliminary annotation.

  6. Secondary infection with Streptococcus suis serotype 7 increases the virulence of highly pathogenic porcine reproductive and respiratory syndrome virus in pigs

    Directory of Open Access Journals (Sweden)

    Xu Min

    2010-08-01

    Full Text Available Abstract Background Porcine reproductive and respiratory syndrome virus (PRRSV and Streptococcus suis are common pathogens in pigs. In samples collected during the porcine high fever syndrome (PHFS outbreak in many parts of China, PRRSV and S. suis serotype 7 (SS7 have always been isolated together. To determine whether PRRSV-SS7 coinfection was the cause of the PHFS outbreak, we evaluated the pathogenicity of PRRSV and/or SS7 in a pig model of single and mixed infection. Results Respiratory disease, diarrhea, and anorexia were observed in all infected pigs. Signs of central nervous system (CNS disease were observed in the highly pathogenic PRRSV (HP-PRRSV-infected pigs (4/12 and the coinfected pigs (8/10; however, the symptoms of the coinfected pigs were clearly more severe than those of the HP-PRRSV-infected pigs. The mortality rate was significantly higher in the coinfected pigs (8/10 than in the HP-PRRSV- (2/12 and SS7-infected pigs (0/10. The deceased pigs of the coinfected group had symptoms typical of PHFS, such as high fever, anorexia, and red coloration of the ears and the body. The isolation rates of HP-PRRSV and SS7 were higher and the lesion severity was greater in the coinfected pigs than in monoinfected pigs. Conclusion HP-PRRSV infection increased susceptibility to SS7 infection, and coinfection of HP-PRRSV with SS7 significantly increased the pathogenicity of SS7 to pigs.

  7. Overcoming function annotation errors in the Gram-positive pathogen Streptococcus suis by a proteomics-driven approach

    Directory of Open Access Journals (Sweden)

    Bárcena José A

    2008-12-01

    Full Text Available Abstract Background Annotation of protein-coding genes is a key step in sequencing projects. Protein functions are mainly assigned on the basis of the amino acid sequence alone by searching of homologous proteins. However, fully automated annotation processes often lead to wrong prediction of protein functions, and therefore time-intensive manual curation is often essential. Here we describe a fast and reliable way to correct function annotation in sequencing projects, focusing on surface proteomes. We use a proteomics approach, previously proven to be very powerful for identifying new vaccine candidates against Gram-positive pathogens. It consists of shaving the surface of intact cells with two proteases, the specific cleavage-site trypsin and the unspecific proteinase K, followed by LC/MS/MS analysis of the resulting peptides. The identified proteins are contrasted by computational analysis and their sequences are inspected to correct possible errors in function prediction. Results When applied to the zoonotic pathogen Streptococcus suis, of which two strains have been recently sequenced and annotated, we identified a set of surface proteins without cytoplasmic contamination: all the proteins identified had exporting or retention signals towards the outside and/or the cell surface, and viability of protease-treated cells was not affected. The combination of both experimental evidences and computational methods allowed us to determine that two of these proteins are putative extracellular new adhesins that had been previously attributed a wrong cytoplasmic function. One of them is a putative component of the pilus of this bacterium. Conclusion We illustrate the complementary nature of laboratory-based and computational methods to examine in concert the localization of a set of proteins in the cell, and demonstrate the utility of this proteomics-based strategy to experimentally correct function annotation errors in sequencing projects. This

  8. Oesophagostomum dentatum and Trichuris suis infections in pigs born and raised on contaminated paddocks

    DEFF Research Database (Denmark)

    Mejer, Helena; Roepstorff, Allan Knud

    2006-01-01

    .p.) the offspring was slaughtered serially every 2 weeks for parasite recovery. Faeces were collected regularly for parasite egg counts and acid-insoluble ash (AIA) content as an indicator of geophagy. Weaning took place week 7 p.p. by removing the sow. Paddock infection levels were estimated in mid-June (O....... dentatum) and late November (O. dentatum and T. suis) using helminth naïve tracer pigs. Soil and vegetation samples were collected regularly. Despite a high initial contamination by the seeder pigs, O. dentatum paddock infectivity was negligible to low throughout the raising of the experimental piglets.......p. The experimental pigs initially had a high faecal level of AIA though it decreased over time. The results are discussed in relation to the biological characteristics of the 2 parasites and their occurrence in organic pig production....

  9. A Randomised, Double-blind, Placebo-controlled Trial of Trichuris suis ova in Active Crohn's Disease

    DEFF Research Database (Denmark)

    Schölmerich, Jürgen; Fellermann, Klaus; Seibold, Frank W

    2017-01-01

    Background and Aims: To investigate the efficacy and safety of three different dosages of embryonated, viable eggs of Trichuris suis [TSO] versus placebo for induction of remission in mildly-to-moderately active ileocolonic, uncomplicated Crohn's disease [CD]. Methods: Adults with active CD [n.......2%, and 47.2% of TSO 250, TSO 2500, and TSO 7500 patients, respectively, and in 42.9% of placebo recipients. TSO induced a dose-dependent immunological response. There was no response regarding laboratory markers of inflammation. Other secondary efficacy variables also showed no advantage of TSO over placebo...... for treatment of active CD. Administration of TSO did not result in any serious adverse drug reaction. Review of non-serious suspected adverse drug reactions following TSO did not reveal any safety concerns. Conclusions: Administration of 250-7500 TSO fortnightly over 12 weeks was safe and showed a dose...

  10. Recombinant major outer membrane protein (MOMP) of Chlamydophila abortus, Chlamydophila pecorum, and Chlamydia suis as antigens to distinguish chlamydial species-specific antibodies in animal sera.

    Science.gov (United States)

    Hoelzle, Ludwig E; Hoelzle, Katharina; Wittenbrink, Max M

    2004-10-05

    Recombinant major outer membrane proteins (rMOMP) of Chlamydophila (Ch.) abortus, Ch. pecorum, and Chlamydia (C.) suis were used as antigens to distinguish chlamydial species-specific antibodies in (i) immune sera from six rabbits and three pigs raised against native purified elementary bodies, (ii) serum samples from 25 sows vaccinated with Ch. abortus, and (iii) 40 serum samples from four heifers experimentally infected with Ch. abortus. All post-exposition sera contained chlamydial antibodies as confirmed by strong ELISA seroreactivities against the chlamydial LPS. For the rMOMP ELISA mean IgG antibody levels were at least 5.8-fold higher with the particular rMOMP homologous to the chlamydial species used for immunisation or infection than with heterologous rMOMPs (P <0.001). Preferential rMOMP ELISA reactivities of sera were confirmed by Western blotting. The results suggest that the entire chlamydial rMOMP could provide a species-specific serodiagnostic antigen.

  11. Je suis un zombie : Études récentes sur Richard Matheson et le mort-vivant

    OpenAIRE

    Ransom, Amy J.; Trudel, Jean-Louis

    2013-01-01

    Dans ce texte nous résumons quatre essais récents sur le phénomène actuel du zombie ainsi qu’un collectif sur l’œuvre de Richard Matheson dont la première version filmique du roman Je suis une légende a servi d’inspiration à l’ur-film de zombies, La nuit des morts vivants de George A. Romero. Tandis que Pierre Cassou-Noguès et Maxime Coulombe signent des traitements plus philosophiques du zombie, leurs ouvrages diffèrent beaucoup dans leur forme et leur fonction. Pour leur part, Amélie Pépin ...

  12. Die Nederduitsch Hervormde Kerk van Afrika: 'n Vrywillige ...

    African Journals Online (AJOL)

    The Nederduitsch Hervormde Kerk van Afrika: A voluntary association or an association sui generis? The Nederduitsch Hervormde Kerk is seen as a voluntary association in South African law which is founded on a contractual basis. Recent case law has re-affirmed this fact. This was not always the case. Earlier case law ...

  13. The "Emerging" and "Emergent" Present: A View on the Indeterminate Nature of Mathematics Lessons

    Science.gov (United States)

    Roth, Wolff-Michael; Maheux, Jean-François

    2014-01-01

    The notion of emergence has considerable currency in mathematics education. However, the notion tends to be used in a descriptive way rather than being theorized and developed as a phenomenon sui generis. The purpose of this article is to contribute to building a theory of "emergence." After providing an exemplifying description and…

  14. In Search of Museum Professional Knowledge Base: Mapping the Professional Knowledge Debate onto Museum Work

    Science.gov (United States)

    Tlili, Anwar

    2016-01-01

    Museum professionalism remains an unexplored area in museum studies, particularly with regard to what is arguably the core generic question of a "sui generis" professional knowledge base, and its necessary and sufficient conditions. The need to examine this question becomes all the more important with the increasing expansion of the…

  15. EU-UN partnership in military conflict management: Whither the ...

    African Journals Online (AJOL)

    UN) and the European Union (EU) in African conflict management. Constructing the EU as a security actor, including its validation through the UN, is not a process solely based on the normative claims inherent in the EU as a sui generis actor, ...

  16. Psychic blindness or visual agnosia: early descriptions of a nervous disorder.

    Science.gov (United States)

    Baumann, Christian

    2011-01-01

    This article briefly reports on three early contributions to the understanding of visual agnosia as a syndrome sui generis. The authors of the respective papers worked in different fields such as physiology, ophthalmology, and neurology, and, although they were not in direct contact with each other, their results converged upon a consistent view of a nervous disorder that they called psychic blindness.

  17. De la dominación colonial a la fabricación de la nación: Las categorías étnico-raciales en los censos e informes y sus usos políticos en Belice, siglos XIX-XX From Colonial Domination to the Forging of a Nation: Ethnic-racial Categories in Censuses and Reports and their Political Uses in Belize, 19th-20th Centuries

    Directory of Open Access Journals (Sweden)

    Elisabeth Cunin

    2012-04-01

    Full Text Available Este texto presenta un análisis de los procesos de clasificación y categorización étnico-raciales de la población de Belice a lo largo de los siglos XIX y XX, apoyándose en los censos demográficos y en los informes de gobierno. No nos interesamos tanto en las cifras como tales, sino en las categorías de conteo y su evolución, como indicadores de las lógicas políticas de construcción de una sociedad colonial y luego nacional. Mientras que para el XIX los censos dan cuenta de las distintas formas de manejo de la población (transición de la esclavitud a la libertad, afirmación o negación de la diversidad étnico-racial, los informes administrativos dibujan un modelo demográfico-territorial estático y estereotipado como herramienta de gestión política. Para el siglo XX, se analiza el difícil camino hacia la independencia y los cambios introducidos por el nuevo Estado beliceño (categorías, metodología, actores en el proceso de construcción de una "identidad nacional".This text presents an analysis of the processes of erhnic-racial classification and categorization of the population of Belize in the 19th and 20th centuries, based on demographic censuses and government reports. We are not so much interested in figures as such as in the counting categories and their evolution, as indicators of the political logics of constructing a colonial and then a national society. By the 19th century, censuses reflect the different ways of managing the population (transition from slavery to freedom, affirmation or denial of ethnic-racial diversity, the administrative reports outline a static demographic-territorial model stereotypes as a tool for political management. In the 20th century, they analyze the difficult road co independence and the changes introduced by the new Belize state (categories, methodology, actors in the process of constructing a "national identity."

  18. Collection of main international documents on the ethical principles of research and the main regulations on bioethics of the United States and of European Community; Raccolta sui principali documenti internazionali sui principi etici della ricerca e dei principali documenti legislativi degli Stati Uniti e della Comunita` Europea sulla bioetica

    Energy Technology Data Exchange (ETDEWEB)

    Scuderi, G [Istituto Superiore di Sanita` , Rome (Italy). Lab. di Immunologia

    1999-12-31

    The collection includes an Italian translation of the main international documents on ethical principles of scientific research (the Nuremberg Code, the Helsinki Declaration, the Belmont Report) as well as the regulatory text of the United States (the Code of Federal Regulation Title 45, Part 46, that is the 45 Cfr 46) and the regulations of the European Community (the Good Clinical Practice and the Convention for the protection of human rights and dignity of the human being regards to the application of biology and medicine: convention on human rights and biomedicine). The informed consent and the human rights of human subjects involved in a clinical trial are discussed. Moreover, in the regulatory texts, legal rights of particular subjects, i.e. pregnant women, minors, fetuses, embrions, prisoners, are discussed; as well as the bioethics of organ transplantations. [Italiano] Contiene una traduzione in lingua italiana dei principali documenti internazionali sui principi etici della ricerca scientifica: il Codice di Norimberga, la dichiarazione di Helsinki e il rapporto Belmont; il testo legislativo degli Stati Uniti Code of Federal Regulation, capitolo 45 parte 46 (detto il 45 CFR 46) e la normativa della Comunita` Europea relativa alle norme di Good Clinical Practice e alla Convenzione per la protezione dei diritti dell`uomo e la dignita` dei soggetti umani relativamente alle applicazioni della biologia e medicina: Convenzione sui diritti dell`uomo e la biomedicina. Vengono trattati il consenso informato e i diritti umani delle persone che sono coinvolte in una sperimentazione clinica. Inoltre, nei testi normativi ci si riferisce anche ai diritti legali di soggetti particolari, ad esempio: donne in stato di gravidanza, minori, feti, embrioni, prigionieri; viene trattata, infine, l`etica dei trapianti d`organo.

  19. Collection of main international documents on the ethical principles of research and the main regulations on bioethics of the United States and of European Community; Raccolta sui principali documenti internazionali sui principi etici della ricerca e dei principali documenti legislativi degli Stati Uniti e della Comunita` Europea sulla bioetica

    Energy Technology Data Exchange (ETDEWEB)

    Scuderi, G. [Istituto Superiore di Sanita`, Rome (Italy). Lab. di Immunologia

    1998-12-31

    The collection includes an Italian translation of the main international documents on ethical principles of scientific research (the Nuremberg Code, the Helsinki Declaration, the Belmont Report) as well as the regulatory text of the United States (the Code of Federal Regulation Title 45, Part 46, that is the 45 Cfr 46) and the regulations of the European Community (the Good Clinical Practice and the Convention for the protection of human rights and dignity of the human being regards to the application of biology and medicine: convention on human rights and biomedicine). The informed consent and the human rights of human subjects involved in a clinical trial are discussed. Moreover, in the regulatory texts, legal rights of particular subjects, i.e. pregnant women, minors, fetuses, embrions, prisoners, are discussed; as well as the bioethics of organ transplantations. [Italiano] Contiene una traduzione in lingua italiana dei principali documenti internazionali sui principi etici della ricerca scientifica: il Codice di Norimberga, la dichiarazione di Helsinki e il rapporto Belmont; il testo legislativo degli Stati Uniti Code of Federal Regulation, capitolo 45 parte 46 (detto il 45 CFR 46) e la normativa della Comunita` Europea relativa alle norme di Good Clinical Practice e alla Convenzione per la protezione dei diritti dell`uomo e la dignita` dei soggetti umani relativamente alle applicazioni della biologia e medicina: Convenzione sui diritti dell`uomo e la biomedicina. Vengono trattati il consenso informato e i diritti umani delle persone che sono coinvolte in una sperimentazione clinica. Inoltre, nei testi normativi ci si riferisce anche ai diritti legali di soggetti particolari, ad esempio: donne in stato di gravidanza, minori, feti, embrioni, prigionieri; viene trattata, infine, l`etica dei trapianti d`organo.

  20. Expression, purification, crystallization and structure determination of the N terminal domain of Fhb, a factor H binding protein from Streptococcus suis

    Energy Technology Data Exchange (ETDEWEB)

    Zhang, Chunmao [State Key Laboratory of Pathogen and Biosecurity, Beijng Institute of Microbiology and Infectious Disease, No. 20 Dongda Street, Fengtai District, Beijing 100071 (China); Yu, You [Key Laboratory for Protein Sciences of Ministry of Education, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, 100084, Beijing (China); Yang, Maojun, E-mail: maojunyang@tsinghua.edu.cn [Key Laboratory for Protein Sciences of Ministry of Education, Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, 100084, Beijing (China); Jiang, Yongqiang, E-mail: jiangyq@bmi.ac.cn [State Key Laboratory of Pathogen and Biosecurity, Beijng Institute of Microbiology and Infectious Disease, No. 20 Dongda Street, Fengtai District, Beijing 100071 (China)

    2015-10-23

    Fhb is a surface virulence protein from Streptococcus suis, which could aid bacterial evasion of host innate immune defense by recruiting complement regulator factor H to inactivate C3b deposited on bacterial surface in blood. Here we successfully expressed and purified the N terminal domain of Fhb (N-Fhb) and obtained crystals of the N-Fhb by sitting-drop vapor diffusion method with a resolution of 1.50 Å. The crystals belong to space group C2 with unit cell parameters a = 127.1 Å, b = 77.3 Å, c = 131.6 Å, α = 90°, β = 115.9°, γ = 90°. The structure of N-Fhb was determined by SAD method and the core structure of N-Fhb is a β sandwich. We speculated that binding of Fhb to human factor H may be mainly mediated by surface amino acids with negative charges. - Highlights: • We expressed N-Fhb as the soluble protein in Escherichia coli. • Crystals of N-Fhb were grown by sitting drop vapor diffusion method. • Crystals of N-Fhb could diffracted to 1.5 Å. • The core structure of N-Fhb was a β sandwich. • A part of the surface of N-Fhb was rich with negative charges.

  1. Acaricidal activity of ethanolic extracts of Stryphnodendron adstringens and Lafoensia pacari and oil resins from Copaifera sp. and Pterodon emarginatus against Sarcoptes scabiei var. suis

    Directory of Open Access Journals (Sweden)

    Adriana Marques Faria

    Full Text Available ABSTRACT: In vitro bioassay using Sarcoptes scabiei as a test microorganism is a viable method of study for diverse drugs with acaricidal properties. A great amount of assays proves the diverse and efficient biological activity of extracts and compounds from Brazilian savanna plants. This study had as main object, test and compare the acaricidal activity of four Brazilian Cerrado bioproducts: Stryphnodendron adstringens Mart., Copaifera sp., Lafoensia pacari A. St Hil. and Pterodon emarginatus Vogel, 1837. To perform this test S. scabiei mites were collected from crusts removed on ears of highly infected sows. The mites were selected and separated in Petri dishes with three different concentrations (25, 50 and 75% of each bioproduct, to evaluate their potential acaricidal activity. The mortality of the mites was counted in each Petri dish every hour, during five hours. The statistical analyses demonstrated differences between the bioproducts tested. The oleoresin of Copaifera sp. and P. emarginatus presented the best results with 100% of mites mortality after treatment. The ethanolic extracts of S. adstringens and L. pacari demonstrated lower acaricidal activity when compared to the oleoresins, with little or no difference among the control groups tested. This bioassay demonstrated to be efficient, reliable, low cost and easy accomplishment. Oil resins from Copaifera sp. and P. emarginatus have in vitro acaricidal activity against adult females of S. scabiei var. suis.

  2. Application of Well Log Analysis to Assess the Petrophysical Parameters of the Early Eocene Sui Main Limestone (SML in Kharnhak-1 Well, Middle Indus Basin, Pakistan

    Directory of Open Access Journals (Sweden)

    Asad Zia

    2016-04-01

    Full Text Available The petrophysical analysis of the early Eocene Sui Main Limestone (SML has been conducted in Kharnhak-1 well for the prospect of the hydrocarbon exploration of the Khairpur-Jacobabad High, Middle Indus Basin, Pakistan. The petrophysical analysis of SML is carried out on the basis of well logs including gamma ray, spontaneous potential, resistivity, neutron, and density logs. These analyses lead to interpreting the vertical distribution of porosity and permeability in order to measure the reservoir potential of the SML. The Archie equation was used to assess the petrophysical characteristics. The SML has good porosity and poor permeability with positive correlation coefficient between the two parameters. The average volume of shale is 18%. The log signature of SML shows dominance of carbonates (limestone. The reservoir quality of the SML in Kharnhak-1 well is such that it is 77% water saturated. The porosity (x varies inversely with formation resistivity factor (F and compressional wave velocity (Vp. However, F and Vp are directly related with each other. Thus, the electric and elastic properties of the carbonate rocks can be influenced by postdepositional alterations, which include porosity enhancement and reduction processes respectively.

  3. Expression, purification, crystallization and structure determination of the N terminal domain of Fhb, a factor H binding protein from Streptococcus suis

    International Nuclear Information System (INIS)

    Zhang, Chunmao; Yu, You; Yang, Maojun; Jiang, Yongqiang

    2015-01-01

    Fhb is a surface virulence protein from Streptococcus suis, which could aid bacterial evasion of host innate immune defense by recruiting complement regulator factor H to inactivate C3b deposited on bacterial surface in blood. Here we successfully expressed and purified the N terminal domain of Fhb (N-Fhb) and obtained crystals of the N-Fhb by sitting-drop vapor diffusion method with a resolution of 1.50 Å. The crystals belong to space group C2 with unit cell parameters a = 127.1 Å, b = 77.3 Å, c = 131.6 Å, α = 90°, β = 115.9°, γ = 90°. The structure of N-Fhb was determined by SAD method and the core structure of N-Fhb is a β sandwich. We speculated that binding of Fhb to human factor H may be mainly mediated by surface amino acids with negative charges. - Highlights: • We expressed N-Fhb as the soluble protein in Escherichia coli. • Crystals of N-Fhb were grown by sitting drop vapor diffusion method. • Crystals of N-Fhb could diffracted to 1.5 Å. • The core structure of N-Fhb was a β sandwich. • A part of the surface of N-Fhb was rich with negative charges.

  4. Expression of the major outer membrane protein (MOMP) of Chlamydophila abortus, Chlamydophila pecorum, and Chlamydia suis in Escherichia coli using an arabinose-inducible plasmid vector.

    Science.gov (United States)

    Hoelzle, L E; Hoelzle, K; Wittenbrink, M M

    2003-10-01

    The ompA genes encoding the 40 kDa major outer membrane protein (MOMP) of Chlamydophila (Ch.) abortus, Ch. pecorum, and Chlamydia (C.) suis were cloned into the arabinose-inducible plasmid vector pBADMycHis, and recombinant MOMPs (rMOMP) from the three chlamydial species were expressed at high levels in Escherichia (E.) coli. The proteins lacking the 22 aa N-terminal signal peptide were expressed as insoluble cytoplasmic inclusion bodies which were readily purified using immobilized metal-affinity chromatography. The rMOMPs including the N-terminal signal peptide were expressed and translocated as a surface-exposed immunoaccessible protein into the outer membrane of E. coli. Transformants expressing this full-length rMOMP were significantly reduced in viability. Purified native elementary bodies (EB) and rMOMPs of the three chlamydial species purified from the E. coli cytoplasm were used for immunization of rabbits. The resulting sera were analysed for their ability to recognize homologous and heterologous rMOMP and native EB. When testing rMOMP antisera against rMOMP and EB antigens, marked cross-reactivities were detected between the three species. Using EB antisera and rMOMPs as antigens, a significant species-specific reactivity was measured.

  5. PREPARATIVE ISOLATION AND PURIFICATION OF THREE GLYCINE-CONJUGATED CHOLIC ACIDS FROM PULVIS FELLIS SUIS BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY COUPLED WITH ELSD DETECTION.

    Science.gov (United States)

    He, Jiao; Li, Jing; Sun, Wenji; Zhang, Tianyou; Ito, Yoichiro

    2012-01-01

    Coupled with evaporative light scattering detection, a high-speed counter-current chromatography (HSCCC) method was developed for preparative isolation and purification of three glycine-conjugated cholic acids, glycochenodeoxycholic acid (GCDCA), glycohyodeoxycholic acid (GHDCA) and glycohyocholic acid (GHCA) from Pulvis Fellis Suis (Pig gallbladder bile) for the first time. The separation was performed with a two-phase solvent system consisted of chloroform-methanol-water-acetic acid (65:30:10:1.5, v/v/v/v) by eluting the lower phase in the head-to-tail elution mode. The revolution speed of the separation column, flow rate of the mobile phase and separation temperature were 800 rpm, 2 ml/min and 25 °C, respectively. In a single operation, 33 mg of GCDCA, 38 mg of GHDCA and 23 mg of GHCA were obtained from 200 mg of crude extract with the purity of 95.65%, 96.72% and 96.63%, respectively, in one step separation. The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the structures of the three glycine-conjugated cholic acids were identified by ESI-MS, (1)H NMR and (13)C NMR.

  6. Semi-Preparative Isolation and Purification of Three Tauro-Conjugated Cholic Acids from Pulvis Fellis Suis by HSCCC Coupled with ELSD Detection.

    Science.gov (United States)

    He, Jiao; Zhang, Yongmin; Ito, Yoichiro; Sun, Wenji

    2011-01-01

    Coupled with evaporative light scattering detection, a high-speed counter-current chromatography (HSCCC) method was applied to the separation and purification of three tauro-conjugated cholic acids of taurochenodeoxycholic acid (TCDCA), taurohyodeoxycholic acid (THDCA) and taurohyocholic acid (THCA) from Pulvis Fellis Suis (Pig gallbladder bile) for the first time. The two-phase solvent system composed of chloroform-methanol-water-acetic acid (4:4:2:0.3, v/v/v/v) was selected for the one-step separation where the lower phase was used as the mobile phase in the head to tail elution mode. The revolution speed of the separation column, flow rate of the mobile phase and separation temperature were 800 rpm, 1.5 ml/min and 25°C respectively. From 100 mg of the crude extract, 10.2 mg of TCDCA, 11.8 mg of THDCA and 5.3 mg of THCA were obtained with the purity of 94.6%, 96.5% and 95.4%, respectively. in one step separation The HSCCC fractions were analyzed by high-performance liquid chromatography (HPLC) and the structures of the three tauro-conjugated cholic acids were identified by ESI-MS, (1)H NMR and (13)C NMR.

  7. A Review of Comparison of Complications of Vaginal Hysterectomy with and without Concomitant Surgery for SUI: A 5 Years’ Experience at a Tertiary Care Hospital of Pakistan

    Directory of Open Access Journals (Sweden)

    Raheela Mohsin Rizvi

    2013-01-01

    Full Text Available Objective. The study was performed to review the complications of surgery for POP with or without surgery for SUI. This included the need for second procedure two years after the primary surgery. Study Design. We conducted a retrospective cross-sectional comparative study at the Aga Khan University, Karachi, Pakistan. International Classification of Diseases, 9th revision, Clinical Modification (ICD-9-CM was used to identify women who underwent vaginal hysterectomy with anterior/posterior repair alone and those with concomitant tension-free vaginal tape surgery for urodynamic stress incontinence. Results. The 28 cases of VH/repair combined with TVT were compared for complications with 430 cases of VH with repair alone. The basic characteristics like age, BMI, and degree of prolapse showed no statistical difference among two groups. The main comorbidities in both groups were hypertension, diabetes, and bronchial asthma. We observed no significant differences in intraoperative and postoperative complications except for cuff abscess, need for medical intervention, and readmission following discharge from hospital, which were higher in cases with vaginal hysterectomy with concomitant TVT. Conclusions. Vaginal hysterectomy is an efficient treatment for uterovaginal prolapse with a swift recovery, short length of hospital stay, and rare serious complications. The addition of surgery for USI does not appear to increase the morbidity.

  8. 隋前正史医事伦理探骊%Discussion on Medical Ethics Based on the History before the Sui Dynasty

    Institute of Scientific and Technical Information of China (English)

    赵仁龙; 任杰

    2015-01-01

    征引隋前正史中医学史料,从高层之医学人文关怀、医学教育及医学传播三个伦理方面进行论述,认为:医与政有着天然紧密相连的渊源关系,医政充分体现了政府有无政治作为和是否实施人文关怀;汉魏南北朝时期太学、地方官学、私学都很活跃,在医学教育方面,也有深刻的进步理念;中医药作为加强民族间交流和融合的友好使者,对当今的中国优秀传统文化对外传播有着重要的引领意义。%Based on the TCM historical information before the history of Sui Dynasty, from the top of the medi-cal humanistic care, medical education and medical communication, the essay discusses three ethical, think: the medical is closely combined with politics have a natural origin, medical administration embodies the government whether any politics as and humanistic care;Jun business, local authority, private and southern and northern dy-nasties period is active, in terms of medical education, also has a profound progress idea; Chinese medicine as strengthen communication and integration between national goodwill ambassador, in today′s Chinese excellent tradi-tional culture of foreign communication has important guide meaning.

  9. AB194. A 12-year retrospective evaluation of TVT and TVT-O in the surgical management of SUI in females and the analysis of influence factors

    Science.gov (United States)

    Zhu, Kejia; Liu, Yaxiao; Chen, Fan; Cheng, Shanshan; Yang, Yue; Shi, Benkang

    2016-01-01

    Objective We evaluate the clinical effect of TVT and TVT-O slings in the surgical management of SUI in women at mostly 12-year follow-ups and the factors that influence it. Methods In this retrospective analysis, 94 women with stress incontinence treated by TVT (14 cases) or TVT-O (80 cases) were included. Subjective evaluation included ICIQ-SF, KHQ, PGI-I and patient satisfaction, and we recorded the objective data of the procedures. We analyzed whether the age, BMI, disease duration, surgery history, postoperative follow-up duration or other factors were related to the subjective cure rate. Results Eight cases of TVT and 59 cases of TVT-O completed the follow-up. Patients were followed up for 1.3–12 years in TVT and 0.2–9.2 years in TVT-O group. The rates of subjectively assessed success were 87.5% and 84.7%, respectively (P=1.00). The total KHQ were (21.93±30.42) and (16.89±25.85) (P=0.55). The operation duration were (59.29±21.02) in TVT group and (29.87±12.04) in TVT-O group (P=0.00). The postoperative days of indwelling catheter were (4.14±1.79) and (3.11±1.51) (P=0.02), and the post-operation hospitalization days were (5.64±2.06) and (5.01±1.77) (P=0.27). The factors above didn’t affect the subjective cure rate. Conclusions The patient reported long-term success rate were both high and had no significant differences between the groups. Operation duration and postoperative durations of indwelling catheter of TVT-O was shorter. The factors above didn’t affect the subjective cure rate.

  10. SUI LIMITI DELLA DIGNITÀ KANTIANA

    Directory of Open Access Journals (Sweden)

    ADRIAN MĂGDICI

    2016-11-01

    Full Text Available In contemporary culture, due to a loss of transcendence and the spread of the so-called ‘Hume's law’ (the famous is-ought problem, there is ever more propagation of the idea that morality cannot be deduced from ‘on high’ (metaphysics, nor from ‘below’ (empiric reality. The demarcation between good and bad, in other words, would be the outcome of a mere human convention. It is evident therefore that even the concept of ‘dignity’ appears like a type of empty and arbitrary intellectualization. Starting with Kant, in fact, the ancient invitation of the Stoics to act in conformity with the rationality-divinity of the cosmos and the successive foundation of morality based in a transcendent source were substituted with the imperative of ‘autonomous’ reason, of that reason which would participate, with its universal moral law, in the “kingdom of ends”. The Kantian foundation of dignity, however, is very controversial in our days because the so-called ‘autonomy’ reveals itself as too subjective and weak, conditioned by the environment and social conventions; those who pay the consequences are the people who mostly need to be defended, the disinherited of history, that is, human beings without a face and voice.

  11. SUI LIMITI DELLA DIGNITÀ KANTIANA

    OpenAIRE

    ADRIAN MĂGDICI

    2016-01-01

    In contemporary culture, due to a loss of transcendence and the spread of the so-called ‘Hume's law’ (the famous is-ought problem), there is ever more propagation of the idea that morality cannot be deduced from ‘on high’ (metaphysics), nor from ‘below’ (empiric reality). The demarcation between good and bad, in other words, would be the outcome of a mere human convention. It is evident therefore that even the concept of ‘dignity’ appears like a type of empty and arbitrary intellectualizat...

  12. No Need to Get Emotional? Emotions and Heuristics

    OpenAIRE

    Szigeti, Andras

    2013-01-01

    Many believe that values are crucially dependent on emotions. This paper focuses on epistemic aspects of the putative link between emotions and value by asking two related questions. First, how exactly are emotions supposed to latch onto or track values? And second, how well suited are emotions to detecting or learning about values? To answer the first question, the paper develops the heuristics-model of emotions. This approach models emotions as sui generis heuristics of value. The empirical...

  13. Metamathematics of fuzzy logic

    CERN Document Server

    Hájek, Petr

    1998-01-01

    This book presents a systematic treatment of deductive aspects and structures of fuzzy logic understood as many valued logic sui generis. Some important systems of real-valued propositional and predicate calculus are defined and investigated. The aim is to show that fuzzy logic as a logic of imprecise (vague) propositions does have well-developed formal foundations and that most things usually named `fuzzy inference' can be naturally understood as logical deduction.

  14. Emergent emotion

    OpenAIRE

    O'Connell, Elaine Finbarr

    2016-01-01

    I argue that emotion is an ontologically emergent and sui generis. I argue that emotion meets both of two individually necessary and jointly sufficient conditions for ontological emergence. These are, (i) that emotion necessarily has constituent parts to which it cannot be reduced, and (ii) that emotion has a causal effect on its constituent parts (i.e. emotion demonstrates downward causation).\\ud \\ud I argue that emotion is partly cognitive, partly constituted by feelings and partly perceptu...

  15. Daniel Gallego Hernández (Ed.. Enfoques actuales en traducción económica e institucional. Actas del Congreso Internacional de Traducción Económica, Comercial, Financiera e Institucional. Suiça: Editorial Peter Lang, 2015. 254 p.

    Directory of Open Access Journals (Sweden)

    Miguel Tolosa Igualada

    2016-05-01

    Full Text Available http://dx.doi.org/10.5007/2175-7968.2016v36n2p291 Daniel Gallego Hernández (Ed.. Enfoques actuales en traducción económica e institucional. Actas del Congreso Internacional de Traducción Económica, Comercial, Financiera e Institucional. Suiça: Editorial Peter Lang, 2015. 254 p.

  16. L'evoluzione dei generi televisivi nei principali paesi europei ed extraeuropei

    OpenAIRE

    Granelli, Susanna; Saxer, Ulrich

    2005-01-01

    This research examines the temporal evolution of television broadcasting genres, focusing on a comparative analysis of these in the major European and non-European countries, with special attention to Italy. One of the principal objectives of the research is to highlight the similarities and differences between such genres, and to map out their future developments. An ulterior objective is to verify the theoretical relevance of the concept of “genre” in the sector of television communication....

  17. I generi letterari nell’estetica crociana, fra tradizione e attualità

    NARCIS (Netherlands)

    Pennings, L.

    2009-01-01

    Proposals for a modern concept of genre in the aesthetics of Benedetto Croce From an essay of 1894 up to his last writings of 1952, Benedetto Croce, whose philosophy dominated fifty years of Italian aesthetics and criticism, denied the value of literary genres. While Croce’s veto undoubtedly caused

  18. Crescere nella migrazione. Generi e sessualità fra gli adolescenti di origine straniera

    Directory of Open Access Journals (Sweden)

    Federica Tarabusi

    2015-08-01

    Full Text Available How do the children of immigrants define and represent themselves in the Italian society? How do they build and negotiate their own subjectivity of women/men and experience their sexual bodies in adolescence? In what way their everyday experiences are different from (or similar to those of Italian teenagers?Grounded on an ethnographic work carried out in the context of Bologna and surrounding area, the paper investigates the symbolic construction of gender and sexuality among the teenagers of foreign origin. Exploring their multiple biographical trajectories, the research reveals a pattern of resources, subjective desires, imaginaries largely shared with the younger generations, but also their multiple attempts to negotiate and challenge both parent’s expectations and Italian society’s request of “assimilation”. Giving voices to the children of immigrants, the empirical data helps us to deconstruct, through the lens of sexuality, gender and bodily practices, essentialist ideas and stereotypical representations attached to the so-called second generations as well as to discuss the role play by structural inequalities and exclusionary processes in shaping their experience of being adolescents. 

  19. Sociosemiotica e generi comunicativi: una proposta per analizzare la comunicazione nel web

    Directory of Open Access Journals (Sweden)

    Nicola Cavalli

    2003-12-01

    Full Text Available Il web può essere definito come uno spazio antropologico, uno spazio di significati caratterizzato dalla rivoluzione dei concetti spazio/temporali. Per analizzare la comunicazione web con un modello che possa essere valido anche per altri tipi di comunicazione viene proposto l'approccio sociosemiotico. In quest'ambito viene sviluppato il concetto di genere comunicativo, che vuole essere la chiave per analizzare la comunicazione e trarre indicazioni sulle carateristiche sociali della comunità di riferimento.

  20. Uma tipologia dos estabelecimentos agropecuários do Brasil a partir do censo de 2006

    Directory of Open Access Journals (Sweden)

    Angela Antonia Kageyama

    2013-03-01

    Full Text Available Baseando-se na composição da mão de obra utilizada, medida a partir do número de pessoas e do tempo de trabalho no ano, os estabelecimentos agropecuários foram agrupados em quatro categorias: assentado, exclusivamente familiar, familiar com contratado, não familiar. Os estabelecimentos de "assentados" são em quase sua totalidade estabelecimentos familiares; os "exclusivamente familiares" são estabelecimentos conduzidos pelo produtor exclusivamente com mão de obra familiar (pessoas com laço de parentesco com o produtor, sem pessoal contratado; os "familiares com contratados" utilizam pessoas da família e pessoal contratado, porém a mão de obra familiar supera a dos contratados; os estabelecimentos "não familiares" incluem os que operam somente com trabalho contratado e aqueles em que a força de trabalho contratada supera a dos membros da família do produtor. Foram analisadas as características de área, valor da produção, produtividade e fontes de receitas. Os estabelecimentos familiares são predominantes (90% e abrigam 80% das pessoas ocupadas na agricultura, mas geram somente 50% do valor produzido. A concentração fundiária dentro das categorias familiares é tão elevada quanto nos estabelecimentos não familiares. No Brasil, apenas 16% da desigualdade da distribuição da terra se deve à desigualdade entre as categorias analisadas; os 84% restantes devem-se à desigualdade dentro dos grupos.

  1. Indagine critica sui modelli di atmosfera

    Directory of Open Access Journals (Sweden)

    G. D'ELIA

    1964-06-01

    Full Text Available Nella prima parte del lavoro E individuato il modello
    piu attendibile di atmosfera, clie deve dunque essere adottato nei calcoli
    relativi a problemi aeronautici e missilistici.
    Nella seconda parte vengono scritte le equazioni deH'atmosfera considerata
    come campo fluidodinamico costituito da piu componenti elettricamente
    conduttivi e reagenti. Con una serie di successive approssimazioni
    sono derivate le equazioni sempliflcate clie attualmente sono usate per il
    calcolo delle tabelle dell'atmosfera; da un sistema complesso di equazioni,
    ma determinato matematicamente, si ottiene un sistema semplice, ma doppiamente
    indeterminato; alia inderminatezza si deve sopperire con sondaggi
    sperimentali.
    Nell'ultima parte del lavoro si fa una critica alia base di calcolo del
    piu attendibile modello, rilevando tra l'altro che esso e relativo alle condizioni
    di un punto singolare (Port Churchill, nel Canada posto in prossimita
    del polo magnetico.

  2. Sui monosillabi nel pentametro: elegia ed epigramma

    OpenAIRE

    Magnelli, E.

    2012-01-01

    Questo studio offre un’analisi di prima mano sull’uso di monosillabi alla fine del primo emisticho del pentametro, nell’elegia e nell’epigramma greco fino all’età di Giustiniano. Nessun tipo di restrizione sembra riguardare i monosillabi appartenenti a una ‘parola metrica’. I dati riguardanti i monosillabi indipendenti – ossia non appositivi e non preceduti da un’appositiva – sono meno facili da interpretare, e non è chiaro se i poeti greci cercassero di evitarne la collocazione i...

  3. [)IE SUI[).AFRIKAA~SE LEERKOLLEGE

    African Journals Online (AJOL)

    van die gebou waarin die skool gehuisves is ondersoek word. Net na die verowering van Pretoria in 1900 het die Britse besettingsmagte. 'n soldate- kantien op die ou Markplein aan die Kerk- straatkant in Pretoria opgerig. In die twee jaar wat daarop gevolg het, is 'n wins van. £20000 gemaak en het lord Kitchener besluit.

  4. A legal institutional perspective on the European Union External Action Service

    DEFF Research Database (Denmark)

    Van Vooren, Bart

    2010-01-01

    It is beyond doubt that setting up the European External Action Service will have a deep impact on EU external policy making. Both in legal and policy terms, this new player thoroughly changes the institutional balance in EU external relations. The goal of this paper is to examine the legal side...... of that coin, by exploring the legal and institutional nature and position of the EEAS in the EU’s external relations machinery. To that end, it queries the meaning of the EEAS’ sui generis status in the EU institutional set-up: what does it mean to say that the EEAS is ‘functionally autonomous’ from...

  5. Post Rule of Law

    DEFF Research Database (Denmark)

    Carlson, Kerstin Bree

    2016-01-01

    The value of developing hybrid international criminal procedure (ICP) is that it is arguably inclusive (representing two major legal traditions) and distinct from any domestic system, thus creating a separate, sui generis realm for international criminal law (ICL) jurists to meet. Since its...... addresses the practice of hybridity in ICP, drawing examples from the construction and evolution of hybrid procedure at the International Criminal Tribunal for the Former Yugoslavia (ICTY), to argue that the hybridity practiced by international criminal tribunals renders them ‘post rule of law’ institutions...

  6. Sensory Substitution and Multimodal Mental Imagery.

    Science.gov (United States)

    Nanay, Bence

    2017-09-01

    Many philosophers use findings about sensory substitution devices in the grand debate about how we should individuate the senses. The big question is this: Is "vision" assisted by (tactile) sensory substitution really vision? Or is it tactile perception? Or some sui generis novel form of perception? My claim is that sensory substitution assisted "vision" is neither vision nor tactile perception, because it is not perception at all. It is mental imagery: visual mental imagery triggered by tactile sensory stimulation. But it is a special form of mental imagery that is triggered by corresponding sensory stimulation in a different sense modality, which I call "multimodal mental imagery."

  7. Singapura, uma questão de identidade? : de cidade portuária a cidade global

    OpenAIRE

    Santos, Dimas Fernando Gomez

    2016-01-01

    Dissertação de Mestrado Integrado em Arquitectura, apresentada ao Departamento de Arquitectura da Faculdade de Ciências e Tecnologia da Universidade de Coimbra. Singapura, cidade-estado com forte acento global, resulta de uma multitude de temporalidades que produzem e reproduzem um tipo de organização sui generis. O ritmo de desenvolvimento frenético do país nas últimas décadas derivou num estado de palimpsesto e num défice de património urbano e ambiental, destoantes da “tradição” ocident...

  8. On Law and Policy in a European and European Union Patent Court (EEUPC)

    DEFF Research Database (Denmark)

    Schovsbo, Jens Hemmingsen; Petersen, Clement Salung

    On 4 December 2009, the European Council unanimously adopted conclusions on an enhanced patent system in Europe, which inter alia intends to establish a new EU patent as well as a new common patent judiciary - the European and European Union Patent Court (EEUPC). The EEUPC will constitute a new sui...... generis, transnational court system with exclusive jurisdiction in respect of civil litigation related to the infringement and validity of European patents and EU patent. This paper considers this proposal for the establishment of the EEUPC under two basic observations, namely that substantive law...

  9. Politics, culture, and the legitimacy of disease: the case of Buerger's disease.

    Science.gov (United States)

    Lockwood, Stephen J; Bresler, Scott C; Granter, Scott R

    2016-09-01

    Thromboangiitis obliterans (TAO) or Buerger's disease is a rare form of vasculitis with distinctive clinical and pathological features that carries significant morbidity, often leading to amputation, and is strongly associated with tobacco smoking. Despite its distinctive clinicopathological characteristics, the existence of TAO as an entity sui generis was challenged for many years as it languished in relative obscurity. Then, as societal attitudes towards smoking changed, TAO not only became accepted as a disease entity, it quite literally became a poster child to illustrate the ills of smoking. Herein, we examine the history of TAO to illustrate the power of societal attitudes and politics in shaping medicine.

  10. Powieść SF uwalniająca się od politycznych serwitutów

    OpenAIRE

    Ryszard Handke

    2015-01-01

    Science-Fiction Novel Liberates Itself from Political Dues The present issue of "Colloquia Humanistica" contains Professor Ryszard Handke's two last essays, until now unpublished. They belong together and deal with the works of Stanisław Lem, namely with the creation of a sui generis dictionary of this outstanding sci-fi writer. Handke highlights the coming of a new age in the evolution of the genre, already foreshadowed in Lem's early novels. This new sci-fi abandons uncritical beliefs i...

  11. Narzędzia językowe w sytuacji ekstremalnej. Stanisława Lema fantastyczne światy ze słów

    OpenAIRE

    Ryszard Handke

    2015-01-01

    Linguistic Tools in an Extreme Situation. Stanisław Lem’s Fantastic Worlds Built with Words The present issue of "Colloquia Humanistica" contains Professor Ryszard Handke's two last essays, until now unpublished. They belong together and deal with the works of Stanisław Lem, namely with the creation of a sui generis dictionary of this outstanding sci-fi writer. Handke highlights the coming of a new age in the evolution of the genre, already foreshadowed in Lem's early novels. This new sci...

  12. Thinking Globally, Acting Locally: Joel Wegmeister and Modern Hasidic Politics in Warsaw

    Directory of Open Access Journals (Sweden)

    François Guesnet

    2011-10-01

    Full Text Available This contribution investigates how the emergence of the first modern Jewish metropolis in Warsaw in the second half of the 19th century challenged traditional visions of community cohesion. It argues that the acceleration of political and societal change within the Jewish community allowed observant elites to achieve political and cultural hegemony in Warsaw, and thus offers a sui generis pathway of Jewish metropolitan modernization. This claim is substantiated by following the communal and political involvement of a leading Hasidic civil leader, Joel Wegmeister (1837-1919, co-founder of the first outlets of the Agudat Israel in the Kingdom of Poland before World War One

  13. The international implications of the Chinese model of development in the Global South: Asian Consensus as a network power

    Directory of Open Access Journals (Sweden)

    Javier Vadell

    2014-01-01

    Full Text Available This paper analyzes People's Republic of China (PRC economic and political ascendance in the 21st century focusing on the evolution of the sui generis economic development model and its significances of the evolution of relationship between China and the developing countries in the peripheral "Global South." The objective of this article is to analyze the relationship between China and the Global South (Africa and South America in the 21st century, characterized as a new Center-periphery global network power based on trade and investment that we call as "Asian Consensus."

  14. Endocrinological-radiological aspects of galactorrhea

    International Nuclear Information System (INIS)

    Muehlenstedt, D.; Boettcher, H.D.; Osmers, F.; Muenster Univ.

    1983-01-01

    The extrapuerperal secretion of the mammary glands is not a pathological image sui generis, but principally a symptom of a different disease to be detected. On the one hand this procedure must consider differential-diagnostic assessments of the endocrinium and primarily consider disorders of the prolactine metabolism or release. On the other hand, morphologic intraductal processes must be excluded by means of concerted X-ray diagnostics. A clearly defined diagnostic programme permits the concerted treatment of mammary gland secretion according to the causes. Only the systematic progress of the examination offers a sufficient degree of security that fundamental pathologic findings as pituitary adenomas or intraductal carcinomas are not overlooked. (orig.) [de

  15. De plano piloto a metrópole : entraves e desafios da política habitacional de Brasília

    OpenAIRE

    Barbosa Júnior, Mauro Ribeiro

    2016-01-01

    Esta dissertação tem por objetivo analisar as singularidades e externalidades que impactam na política habitacional do Distrito Federal, considerando os seguintes aspectos: a trajetória da política habitacional brasileira e distrital, a expansão urbana de Brasília e os limites da gestão metropolitana. O fenômeno da habitação em Brasília é um caso sui generis de planejamento urbano no Brasil, dada a complexidade urbana, ambiental e política que envolve a concretização do acesso a moradia. Ness...

  16. “Wearing the Clothing of the Saint". Afro Characteristics in the Personality of San Baltazar through some Rites in his Cult in Argentina

    OpenAIRE

    Cirio, Norberto Pablo

    2014-01-01

    Through its history, one can see traces of African roots in the popular cult of San Baltazar in Argentina. In spite of starting as an imposed devotion by the clergy and the Spanish Crown to the slaves in the middle of 18th Century, early the later could insert their own cultural traditions, producing a sort of sui generis syncretism. Now days, those traditions are present in some religious ceremonies, especially in the devotional area. So far they consider that he is the patron saint of the j...

  17. “Vistiendo las ropas del santo”: Atributos afro en la personalidad de san Baltasar a través de algunos cargos devocionales en su culto en la Argentina

    OpenAIRE

    Norberto Pablo Cirio

    2003-01-01

    Through its history, one can see traces of African roots in the popular cult of San Baltazar in Argentina. In spite of starting as an imposed devotion by the clergy and the Spanish Crown to the slaves in the middle of 18th Century, early the later could insert their own cultural traditions, producing a sort of sui generis syncretism.Now days, those traditions are present in some religious ceremonies, especially in the devotional area. So far they consider that he is the patron saint of the jo...

  18. El conocimiento indígena asociado a los recursos de biodiversidad y su implicación en el sistema de derechos de propiedad intelectual: una mirada desde la perspectiva indígena

    OpenAIRE

    Soria Java, José

    2013-01-01

    El reconocimiento de los “derechos sobre la biodiversidad” en el sistema internacional implica su comercialización creando un nuevo paradigma de poder. Dicho reconocimiento lesiona los derechos de quienes siempre han convivido con la “madre tierra” y que generalmente han utilizado los recursos de la biodiversidad para su pervivencia como pueblos. Los derechos de propiedad intelectual y el sistema sui generis1 son contrarios a las aspiraciones de nuestros pueblos indígenas, quienes ven en e...

  19. Wahrnehmung und Explikation

    DEFF Research Database (Denmark)

    Jardine, James Alexander

    2016-01-01

    Within the phenomenological tradition, one frequently finds the bold claim that interpersonal understanding is rooted in a sui generis form of intentional experience, most commonly labelled empathy (Einfühlung). The following paper explores this claim, emphasizing its distinctive character......, in section 3, the Husserlian claim that certain forms of empathy are perceptual in nature, and in section 4, Stein’s insistence that empathetic experience frequently involves explicating the other’s own intentional experiences. Section 5 will conclude by assessing the extent to which their analyses lend...

  20. On Law and Policy in a European and European Union Patent Court (EEUPC)

    DEFF Research Database (Denmark)

    Petersen, Clement Salung; Schovsbo, Jens Hemmingsen

    2010-01-01

    On 4 December 2009, the European Council unanimously adopted conclusions on an enhanced patent system in Europe, which inter alia intends to establish a new EU patent as well as a new common patent judiciary – the European and European Union Patent Court (EEUPC). The EEUPC will constitute a new sui...... generis, transnational court system with exclusive jurisdiction in respect of civil litigation related to the infringement and validity of European patents and EU patent. This paper considers this proposal for the establishment of the EEUPC under two basic observations, namely that substantive law...

  1. Neural computation and the computational theory of cognition.

    Science.gov (United States)

    Piccinini, Gualtiero; Bahar, Sonya

    2013-04-01

    We begin by distinguishing computationalism from a number of other theses that are sometimes conflated with it. We also distinguish between several important kinds of computation: computation in a generic sense, digital computation, and analog computation. Then, we defend a weak version of computationalism-neural processes are computations in the generic sense. After that, we reject on empirical grounds the common assimilation of neural computation to either analog or digital computation, concluding that neural computation is sui generis. Analog computation requires continuous signals; digital computation requires strings of digits. But current neuroscientific evidence indicates that typical neural signals, such as spike trains, are graded like continuous signals but are constituted by discrete functional elements (spikes); thus, typical neural signals are neither continuous signals nor strings of digits. It follows that neural computation is sui generis. Finally, we highlight three important consequences of a proper understanding of neural computation for the theory of cognition. First, understanding neural computation requires a specially designed mathematical theory (or theories) rather than the mathematical theories of analog or digital computation. Second, several popular views about neural computation turn out to be incorrect. Third, computational theories of cognition that rely on non-neural notions of computation ought to be replaced or reinterpreted in terms of neural computation. Copyright © 2012 Cognitive Science Society, Inc.

  2. Protecting traditional knowledge of Chinese medicine: concepts and proposals.

    Science.gov (United States)

    Liu, Changhua; Gu, Man

    2011-06-01

    With the development of the knowledge economy, knowledge has become one of the most important resources for social progress and economic development. Some countries have proposed measures for the protection of their own traditional knowledge. Traditional Chinese medicine belongs to the category of intangible cultural heritage because it is an important part of Chinese cultural heritage. Today the value of traditional knowledge of Chinese medicine has been widely recognized by the domestic and international public. This paper discusses the definition of traditional knowledge of Chinese medicine and its protection, and evaluates research on its classification. We review the present status of the protection of traditional knowledge of Chinese medicine and tentatively put forward some possible ideas and methods for the protection of traditional knowledge of Chinese medicine. Our goal is to find a way to strengthen the vitality of traditional Chinese medicine and consolidate its foundation. We believe that if we could establish a suitable sui generis(sui generis is a Latin term meaning "of its own kind" and is often used in discussions about protecting the rights of indigenous peoples. Here we use it to emphasize the fact that protection of traditional knowledge of Chinese medicine cannot be achieved through existing legal means of protection alone due to its unique characteristics) system for traditional knowledge, a more favorable environment for the preservation and development of traditional Chinese medicine will ultimately be created.

  3. THE RELEVANCE OF SOCIO-LEGAL STUDIES IN LEGAL SCIENCE

    Directory of Open Access Journals (Sweden)

    Victor Imanuel W. Nalle

    2015-02-01

    Full Text Available Some law schools in Indonesia reject socio-legal studies with epistemological arguments that puts jurisprudence as sui generis. Rejection is based argument that jurisprudence is a normative science. In fact socio-legal studies in the development of jurisprudence outside Indonesia has long existed and contributed to the legal reform. Socio-legal studies also significant for legal reform. It is caused by the existence of non doctrinal aspect in law making and implementation of the law. Therefore the position and relevance of socio-legal research is not related to the benefits that provided for the development of national law or jurisprudence. Beberapa fakultas hukum di Indonesia menolak penelitian sosio-legal dengan argumentasi epistemologis yang menempatkan ilmu hukum sebagai sui generis. Penolakan tersebut didasarkan argumentasi bahwa ilmu hukum adalah ilmu yang bersifat normatif. Kenyataannya studi sosio-legal dalam perkembangan ilmu hukum di luar Indonesia telah lama eksis dan berperan dalam pembaharuan hukum. Selain itu, studi sosiolegal juga berperan dalam pembaharuan hukum. Hal ini disebabkan adanya aspek-aspek nondoktrinal yang berperan dalam pembentukan hukum dan implementasi hukum di masyarakat. Oleh karena itu kedudukan dan relevansi penelitian sosio-legal pada ada tidaknya manfaat yang diberikan bagi perkembangan hukum nasional ataupun ilmu hukum.

  4. THE RELEVANCE OF SOCIO-LEGAL STUDIES IN LEGAL SCIENCE

    Directory of Open Access Journals (Sweden)

    Victor Imanuel W. Nalle

    2015-02-01

    Full Text Available Some law schools in Indonesia reject socio-legal studies with epistemological arguments that puts jurisprudence as sui generis. Rejection is based argument that jurisprudence is a normative science. In fact socio-legal studies in the development of jurisprudence outside Indonesia has long existed and contributed to the legal reform. Socio-legal studies also significant for legal reform. It is caused by the existence of non doctrinal aspect in law making and implementation of the law. Therefore the position and relevance of socio-legal research is not related to the benefits that provided for the development of national law or jurisprudence.   Beberapa fakultas hukum di Indonesia menolak penelitian sosio-legal dengan argumentasi epistemologis yang menempatkan ilmu hukum sebagai sui generis. Penolakan tersebut didasarkan argumentasi bahwa ilmu hukum adalah ilmu yang bersifat normatif. Kenyataannya studi sosio-legal dalam perkembangan ilmu hukum di luar Indonesia telah lama eksis dan berperan dalam pembaharuan hukum. Selain itu, studi sosiolegal juga berperan dalam pembaharuan hukum. Hal ini disebabkan adanya aspek-aspek nondoktrinal yang berperan dalam pembentukan hukum dan implementasi hukum di masyarakat. Oleh karena itu kedudukan dan relevansi penelitian sosio-legal pada ada tidaknya manfaat yang diberikan bagi perkembangan hukum nasional ataupun ilmu hukum.

  5. Alcune note sugli effetti dell'inflazione sui bilanci aziendali, e sul problema della rivalutazione monetaria dei beni d'imprese. (Some notes on the effects of inflation on business balance sheets, and on the problem of the appreciation of business assets

    Directory of Open Access Journals (Sweden)

    V. VISCO

    2013-12-01

    Full Text Available Gli effetti dell'inflazione sui bilanci delle imprese negli ultimi anni sono stati oggetto di numerosi studi . Questi hanno evidenziato le distorsioni causate dagli aumenti dei prezzi e hanno suggerito modi per eliminare o mitigarne l'effetto, come l'uso di metodi di indicizzazione , sistemi modificati di contabilità e interventi legislativi specifici e periodici. Il presente lavoro si propone di chiarire la portata degli effetti dell'inflazione sulle voci più significative dei bilanci delle imprese . Inoltre , cerca di analizzare le logiche , le conseguenze e le limitazioni dei meccanismi apprezzamento per beni aziendali adottate in Italia in passato e recentemente proposto di nuovo .The effects of inflation on firms’ balance sheets in recent years have been the subject of numerous studies. These have highlighted the distortions caused by the rises in prices and have suggested ways to eliminate or mitigate them, such as the use of indexing methods, modified systems of accounting and specific and periodic legislative interventions. The present work aims to clarify the scope of the effects of inflation on the most significant items of firms’ balance sheets. Moreover, it seeks to analyse the logic, consequences and limitations of the appreciation mechanisms for business assets adopted in Italy in the past and recently proposed again.JEL: E31, M41

  6. Grandi Eventi: indicatori di classificazione e incidenza sui sistemi urbani

    Directory of Open Access Journals (Sweden)

    Giuseppe Mazzeo

    2008-08-01

    Full Text Available I grandi eventi sono ritenuti una opportunità per le città in quanto sono uno straordinario catalizzatore di investimenti mirati alla trasformazione della città. L’azione dei grandi eventi non è confinata solo nel periodo di svolgimento dell’evento stesso ma si estende ad un periodo molto più ampio, prima e, soprattutto, dopo il suo termine. Nell’organizzazione delle manifestazioni più recenti le trasformazioni urbane hanno interessato in modo sempre maggiore parti già in precedenza urbanizzate, al punto che l’evento diviene l’occasione per trasformare la città costruita e per modificarne il profilo. Il paper approfondisce l’argomento della classificazione degli eventi e del loro impatto sul sistema urbano sviluppandosi in tre parti. Nella prima si analizzano due tipologie di grandi eventi (le esposizioni internazionali e i giochi olimpici con l’obiettivo di pervenire ad una definizione condivisa. Nella seconda parte si identificano i più importanti fattori ed indicatori per l’analisi di questa specifica categoria di avvenimenti. Infine, nella terza parte, si approfondiscono i fattori connessi all’impatto sul sistema urbano e all’organizzazione della sua mobilità. In particolare, viene approfondito il concetto di “effetto pulsar”, ossia gli effetti moltiplicativi sulla evoluzione urbana dipendenti dalla organizzazione di eventi multipli in parallelo o in sequenza. Uno degli elementi più negativi connessi alla organizzazione di un grande evento è la dispersione nel tempo dei benefici acquisiti grazie ad esso; per evitare ciò è necessario che l’evento sia seguito da politiche strategiche continue in modo da preservare i vantaggi acquisiti.

  7. Note critiche sui rapporti tra diritto ed economia

    OpenAIRE

    Tuzet, Giovanni

    2017-01-01

    Il saggio inquadra teoricamente i principali rapporti fra diritto ed economia e analizza i vari metodi e approcci dell'analisi economica del diritto, rilvandone gli aspetti critici e discutendo infine gli approcci comportamentali e la "nudge theory".

  8. Enfermedades Autoinmunes, tratamiento con Trichuris suis y otros helmintos

    Directory of Open Access Journals (Sweden)

    Iñigo Pallardo Fernández

    2015-01-01

    Full Text Available Objetivos: La "Hipótesis de la Higiene" postula sobre los efectos inmunomoduladores inducidos por agentes infecciosos en los seres humanos. El objetivo principal de este trabajo es indagar sobre las evidencias de esa hipótesis y sobre sus aplicaciones en el campo del tratamiento de las enfermedades autoinmunes, haciendo especial hincapié tanto en los mecanismos de acción en los que se basan estas aplicaciones como en los resultados reales obtenidos. Además analizará la posible evolución de estas terapias, especialmente a la sombra de las controversias éticas que surgen de la aplicación de estos tratamientos en relación a si es lícito causar una infección para curar otra patología, como es el caso que nos ocupa. Resultados: Actualmente existen resultados prometedores de ensayos clínicos sobre terapias helmínticas aplicadas al tratamiento de enfermedades autoinmunes como son la enfermedad de Crohn y la colitis ulcerosa, resumidos en el apartado de resultados de este artículo. Igualmente prometedora es la gran variedad de ensayos clínicos que actualmente están en curso sobre la aplicación de la terapia helmíntica al tratamiento de diversas patologías en las que está involucrado el sistema inmunológico, como son: asma, rinitis alérgica, artritis reumatoide, esclerosis múltiple, diabetes Mellitus tipo I, encefalomielitis autoinmune, obesidad, autismo, etc que han llevado a identificar cuáles son los parásitos indicados en el tratamiento de este amplio espectro de enfermedades. Sin embargo es necesario indicar que no todos los helmintos son inmunorreguladores y, por lo tanto útiles en el tratamiento de estas enfermedades y que, los que lo son, no son útiles en el tratamiento de todas las enfermedades de origen inmunológico sino que presentan una marcada especificidad. Es más, la utilidad de éstos presenta una variabilidad importante, no sólo dependiente de la enfermedad de origen inmunológico a tratar, sino también de las condiciones del paciente. Es por ello que actualmente no existen terapias helmínticas aprobadas por las principales agencias del medicamento; aún quedan muchos aspectos por desvelar, lo que hace que la hipótesis de la higiene no haya pasado de ser una hipótesis. Sin embargo lo prometedor de estas terapias ha traído consigo la autorización de algunas de ellas como "productos en fase de investigación clínica". Conclusiones: Es necesario seguir investigando sobre las terapias helmínticas. Además es especialmente prometedor el empleo de productos solubles de parásitos en el tratamiento de estas enfermedades en lugar de la infección helmíntica e incluso, el diseño de análogos sintéticos que sean más efectivos y presenten menos riesgos. La utilización de este último tipo de productos traerá consigo un aumento en el número de ensayos clínicos, justificados por las facilidades de patente de éstos frente a la utilización directa de estados de los parásitos. Sin embargo todavía es necesaria más investigación para conocer las biomoléculas presentes en los parásitos que son responsables de su efecto terapéutico.

  9. Paradossi e realtà saggio sui fondamenti della microfisica

    CERN Document Server

    Selleri, Franco

    1987-01-01

    Impostasi come l'interpretazione ultma e definitiva del mondo fisico, la meccanica quantistica rivela invece ampie falle a un'analisi più attenta dei suoi fondamenti. Con un'argomentazione serrata, Selleri mette a nudo in questo libro le implicite basu filosofiche della teoria dei quanti, e mostra come le più recenti proposte di esperimenti possono rivalutare la posizione realista di Einstein, Schrödinger, de Broglie. Una splendida sintesi di storia e filosofia che ci introduce alle problematiche più attuali della scienza fisica.

  10. Storia, analisi e valutazioni sui mamuthones (I parte

    Directory of Open Access Journals (Sweden)

    Franco Sale

    2010-12-01

    Full Text Available Abstract – IT Franco Sale, cultore delle tradizioni popolari sarde, mamuthone e scultore di maschere, è l’autore di questa “memoria”, scritta tra il febbraio 1997 e l’ottobre 2004, e il depositario consapevole dell’antichissima eredità dei riti del carnevale di Mamojada (Nuoro, Sardegna. Questa consapevolezza è anche la consapevolezza del pericolo dell’adulterazione – dovute alla svalutazione turistica dei contenuti e degli oggetti del rito, allo zelo eccessivo delle associazioni folcloriche, alla negligenza delle amministrazioni, alla scomparsa dei momenti e degli attori della trasmissione orale – che minaccia i riti del carnevale di Mamojada. In questa testimonianza, pubblicata in due parti su questo e sul prossimo numero di “Antropologia e teatro”, Sale ci racconta luoghi e costumi, tracciando una panoramica storica, tra influenze e tentativi di repressione da parte del potere religioso, fino alla faticosa tradizione attuale, protetta “gelosamente” da pochi e restituita alla gente ogni anno con passione e orgoglio, come per una missione. Sale descrive poi l’organizzazione della performance, il ruolo, la vestizione e l’azione dei mamuthones e degli issohadores, i protagonisti del carnevale mamojadino, soffermandosi su elementi di profondo interesse antropologico, come l’uso e il senso dell’indumento femminile indossato dai performer e il vero punto di inizio dell’azione processionale. Il valore di questa testimonianza è dato dalle accurate argomentazioni autobiografiche dell’autore, che ha vissuto in questo mondo fin da bambino e che di esso ci trasmette le sensazioni, attraverso passaggi preziosi in cui rivela l’impressione di un tempo altro nella cornice del carnevale, evocata dalle greggi silenziose, perché private dei campanacci, usati dai mamuthones, e l’impatto emozionale della maschera e del rito. Abstract – EN Franco Sale, expert of the popular traditions of Sardinia, mamuthone and sculptor of masks, is the author of these “memoirs”, written between February 1997 and October 2004, and the conscious heir of the ancient tradition of the rituals of the carnival of Mamojada (Nuoro, Sardinia. This consciousness is also the consciousness of the danger of corruption – caused by touristic exploitation of the ritual contents and items, excessive zeal of folkloristic associations, carelessness from the local institutions, extinction of moments and actors of the oral transmission – which threatens the rituals of the carnival of Mamojada. In this account, published in two parts on this and on the next issue of “Antropologia e teatro”, Sale reports places and mores, describing a historical survey, among influences and repressive attempts of the religious power, until the hard present tradition, “jealously” protected by a minority and proudly performed back to the people of the town, as if it was a mission. Moreover Sale describes the organization of the performance, the roles, the clothing and the action of the mamuthones and the issohadores, the protagonists of the carnival of Mamohada, focusing on elements of deep anthropological interest, such as the use and the meaning of female clothing worn by performers and the actual start time of the procession. In particular this account is worthy thanks to the accurate autobiographical arguments of the author, who lives in this world since childhood and transmits his feelings through precious passages in which he reveals the impression of a different flow of time in the frame of the carnival, evoked by the memory of silent herds, deprived of bells which were used by mamuthones, and the emotional impact of mask and rite.

  11. Chirurgische Therapie der Stressharninkontinenz (SUI - Ein Überblick

    Directory of Open Access Journals (Sweden)

    Riss P

    2009-01-01

    Full Text Available Die Stressharninkontinenz der Frau hat ihre höchste Prävalenz um die Zeit der Menopause. Es gibt verschiedene Erklärungsmöglichkeiten für die Kontinenz bei der Frau: die anatomische Lage des Blasenhalses, die Druckübertragung auf die proximale Urethra, eine Degeneration des M. sphincter urethrae, die sogenannte Hängemattentheorie der Unterstützung des Blasenhalses, das Trampolinmodell von P. Petros und schließlich das Konzept der mittleren Urethra. Auf letzterem beruhen die Operationen, bei denen ein Prolene-Band spannungsfrei unter die mittlere Urethra gelegt wird (TVT und Modifikationen. Wegen der Einfachheit der Durchführung und der exzellenten Erfolgsraten haben diese Operationen die bewährte Kolposuspension nach Burch weitgehend verdrängt. Faszienschlingenoperationen sind speziellen Rezidivsituationen vorbehalten.

  12. SUIS: An Online Graphical Signature-Based User Identification System

    OpenAIRE

    Alam, Shahid

    2016-01-01

    Humans possess a large amount of, and almost limitless, visual memory, that assists them to remember pictures far better than words. This phenomenon has recently motivated the computer security researchers' in academia and industry to design and develop graphical user identification systems (GUISs). Cognometric GUISs are more memorable than drawmetric GUISs, but takes more time to authenticate. None of the previously proposed GUISs combines the advantages of both cognometric and drawmetric sy...

  13. Considerazioni sui lettori di Montesquieu (XVIII-XX secolo

    Directory of Open Access Journals (Sweden)

    Piero Venturelli

    2009-05-01

    Full Text Available This article in meant to bring a contribution to the study of the penetration of Montesquieu’s ideas and theories starting from Montesquieu e i suoi interpreti (a cura di D. Felice, 2 tt., Pisa, ETS, 2005. The most relevant interpretations or uses of the philosopher’s work of the last two hundred and fifty years will be put together with particular reference to the Esprit des Lois (1748. We consider France, Great Britain, North America, Germany and Italy as the linguistic and cultural areas where Montesquieu’s thought is stronger and more lasting. Over forty authors have been taken into consideration: J.-B. le Rond d’Alembert, D. Hume, J.-J. Rousseau, C. Beccaria, A. Genovesi, E. Personè, D. Diderot, L. de Jaucourt, E. Gibbon, J.G. Herder, G. Filangieri, Th. Jefferson, A. Hamilton, J.-A.-N. Caritat de Condorcet, L. Saint-Just, B. Constant, G.W.F. Hegel, A. de Tocqueville, H. Taine, F. Sclopis di Salerano, É.-R. Lefebvre de Laboulaye, F. Meinecke, Ch. Eisenmann, J. Starobinski, L. Althusser, H. Arendt, R. Aron, R. Shackleton etc. In this article, Voltaire, E. Burke, L.-C. Destutt de Tracy, A. Comte, É. Durkheim and S. Cotta have been considered more in detail.

  14. Serological detection of Brucella suis antibodies amongst pigs in ...

    African Journals Online (AJOL)

    Increasing reports of poor production associated with swine brucellosis necessitated this serological study. This study was carried out to establish the prevalence of swine brucellosis in Kaduna state, Nigeria. Three hundred (300) sera were randomly collected from porcine species between July – December, 2012 from ...

  15. Meta-analysis of data from human ex vivo placental perfusion studies on genotoxic and immunotoxic agents within the integrated European project NewGeneris

    DEFF Research Database (Denmark)

    Mose, T; Mathiesen, L; Karttunen, V

    2012-01-01

    highly ranked no matter which parameter was used. Antipyrine normalization resulted in the following ranking order of compounds according to AUC(120NORM) values: NDMA = EtOH = BPA = IQ =AA = GA = PCB180 = PhIP = AFB1 > DON = BP = PCB52 = TCDD. As the variance in all parameters within a study decreased...

  16. Perché i generi alimentari sono meno costosi nei paesi (europei ricchi? (Why Is Food Cheaper in Rich [European] Countries?

    Directory of Open Access Journals (Sweden)

    Alessandro Podkaminer

    2012-04-01

    Full Text Available Relative to non-food items, food tends to be cheaper in rich, as compared with poor European countries. This tendency cannot be explained in terms of cost developments or foreign-trade considerations. A positive explanation proposed focuses on demand-income-supply interaction. An analysis of a cross-country price-augmented modification of Engel's Law, econometrically specified, indicates that the relative price of food is related positively to the supply of food items and negatively to that of non-food items. This finding is consistent with "agricultural price scissors", and also casts a different light on the nature of economic development and structural change.  JEL Codes: D12, L11, L66, Q11Keywords: Food, Prices

  17. Perché i generi alimentari sono meno costosi nei paesi (europei ricchi? (Why Is Food Cheaper in Rich [European] Countries?

    Directory of Open Access Journals (Sweden)

    Alessandro Podkaminer

    2004-09-01

    Full Text Available Relative to non-food items, food tends to be cheaper in rich, as compared with poor European countries. This tendency cannot be explained in terms of cost developments or foreign-trade considerations. A positive explanation proposed focuses on demand-income-supply interaction. An analysis of a cross-country price-augmented modification of Engel's Law, econometrically specified, indicates that the relative price of food is related positively to the supply of food items and negatively to that of non-food items. This finding is consistent with "agricultural price scissors", and also casts a different light on the nature of economic development and structural change.

  18. Maternal diet, prenatal exposure to dioxin-like compounds and birth outcomes in a European prospective mother-child study (NewGeneris).

    Science.gov (United States)

    Papadopoulou, Eleni; Kogevinas, Manolis; Botsivali, Maria; Pedersen, Marie; Besselink, Harrie; Mendez, Michelle A; Fleming, Sarah; Hardie, Laura J; Knudsen, Lisbeth E; Wright, John; Agramunt, Silvia; Sunyer, Jordi; Granum, Berit; Gutzkow, Kristine B; Brunborg, Gunnar; Alexander, Jan; Meltzer, Helle Margrete; Brantsæter, Anne Lise; Sarri, Katerina; Chatzi, Leda; Merlo, Domenico F; Kleinjans, Jos C; Haugen, Margaretha

    2014-06-15

    Maternal diet can result in exposure to environmental contaminants including dioxins which may influence foetal growth. We investigated the association between maternal diet and birth outcomes by defining a dioxin-rich diet. We used validated food frequency questionnaires to assess the diet of pregnant women from Greece, Spain, United Kingdom, Denmark and Norway and estimated plasma dioxin-like activity by the Dioxin-Responsive Chemically Activated LUciferase eXpression (DR-CALUX®) bioassay in 604 maternal blood samples collected at delivery. We applied reduced rank regression to identify a dioxin-rich dietary pattern based on dioxin-like activity (DR-CALUX®) levels in maternal plasma, and calculated a dioxin-diet score as an estimate of adherence to this dietary pattern. In the five country population, dioxin-diet score was characterised by high consumption of red and white meat, lean and fatty fish, low-fat dairy and low consumption of salty snacks and high-fat cheese, during pregnancy. The upper tertile of the dioxin-diet score was associated with a change in birth weight of -121g (95% confidence intervals: -232, -10g) compared to the lower tertile after adjustment for confounders. A small non-significant reduction in gestational age was also observed (-1.4days, 95% CI: -3.8, 1.0days). Our results suggest that maternal diet might contribute to the exposure of the foetus to dioxins and dioxin-like compounds and may be related to reduced birth weight. More studies are needed to develop updated dietary guidelines for women of reproductive age, aiming to the reduction of dietary exposure to persistent organic pollutants as dioxins and dioxin-like compounds. Copyright © 2014 Elsevier B.V. All rights reserved.

  19. Maternal diet, prenatal exposure to dioxin-like compounds and birth outcomes in a European prospective mother–child study (NewGeneris)

    DEFF Research Database (Denmark)

    Papadopoulou, Eleni; Kogevinas, Manolis; Botsivali, Maria

    2014-01-01

    . A small non-significant reduction in gestational age was also observed (-1.4days, 95% CI: -3.8, 1.0days). Our results suggest that maternal diet might contribute to the exposure of the foetus to dioxins and dioxin-like compounds and may be related to reduced birth weight. More studies are needed...

  20. Orthotropic Piezoelectricity in 2D Nanocellulose.

    Science.gov (United States)

    García, Y; Ruiz-Blanco, Yasser B; Marrero-Ponce, Yovani; Sotomayor-Torres, C M

    2016-10-06

    The control of electromechanical responses within bonding regions is essential to face frontier challenges in nanotechnologies, such as molecular electronics and biotechnology. Here, we present Iβ-nanocellulose as a potentially new orthotropic 2D piezoelectric crystal. The predicted in-layer piezoelectricity is originated on a sui-generis hydrogen bonds pattern. Upon this fact and by using a combination of ab-initio and ad-hoc models, we introduce a description of electrical profiles along chemical bonds. Such developments lead to obtain a rationale for modelling the extended piezoelectric effect originated within bond scales. The order of magnitude estimated for the 2D Iβ-nanocellulose piezoelectric response, ~pm V -1 , ranks this material at the level of currently used piezoelectric energy generators and new artificial 2D designs. Such finding would be crucial for developing alternative materials to drive emerging nanotechnologies.

  1. MARKETING ORGANIZATION ACTIVITY IN SPORTS ORGANIZATIONS

    Directory of Open Access Journals (Sweden)

    Slobodan Župljanin

    2012-09-01

    Full Text Available Sports organizations as "sui generis" organizations inevitably have to adopt the concept of marketing as a business philosophy, if you want to survive and develop its activities in the modern business environment. Supply and demand as the market, and psychological phenomena are inherent in sports organizations. Sport is a special area of human activity in which marketing has a specific place and role. Sports and sporting events are also a massive public and media events and high level of interest involving multi-million audience. Between direct and indirect participants of these events takes place appropriate communication sociological, psychological and marketing character. The aim of this paper is to present the possibility of more efficient operation of sports organizations using and adopting the marketing concept as a philosophy of action.

  2. Desain Tata Letak Sirkuit Terpadu sebagai Hak Kekayaan Intelektual dalam Hukum Indonesia

    Directory of Open Access Journals (Sweden)

    Sanusi Bintang

    2018-04-01

    Design of integrated circuits as blue prints for integrated circuit used in various products of information technology, such as computer, cellular phone, and telecomunication media, has its own characteristics which is not fit to be put under the previous intellectual property law regimes, therefore, it needs to be regulate under a specific legislation (sui generis. This research utilizes doctrinal legal research method by applying statute approach and comparative approach. Indonesia has enacted laws on Design of Integrated Circuits, but the law has certain limitations. The limitations is regarding the adequacy of subject matter, the quality of norms, and the legal enforcement. The limitations is not only from the aspect of technical legal drafting, but also rooted on legal culture.

  3. Ação por incumprimento: a técnica do diálogo cooperativo entre o Tribunal de Justiça da União Européia (TJUE) e a jurisdição nacional dos estados-membros

    OpenAIRE

    SOUSA, Leandro Eloy.

    2011-01-01

    Objetivando alcançar níveis mais elevados de desenvolvimento econômico e social alguns países europeus decidiram criar um organismo internacional de caracerísticas sui generis para o qual transferiram parcelas de sua soberania. A formação da União Européia sustenta-se na ordem jurídica comunitária que lhe confere os traços jurídicos fundamentais. A feição adjetiva dessa ordem jurídica diz respeito ao contencioso comunitário cuja concepção busca tutelar e assegurar a normatividade da ordem...

  4. Ticuna traditional knowledge on chagra agriculture and innovative mechanisms for its protection

    Directory of Open Access Journals (Sweden)

    Luis Eduardo Acosta Muñoz

    2012-08-01

    Full Text Available The relationship of indigenous peoples to the global market is a reality and it sets strategies of appropriation of traditional knowledge, without any rule mediating the restitution of rights that knowledge holders have, while indigenous people are asking a sui generis system for protection and equitable participation in these markets. The paper presents the results of a participatory research carried out with Ticuna communities of the south of the Colombian Amazon, in the border with Brazil and Peru, in the upper part of the Amazon River. From the knowledge about the use and management of chagra agriculture, the research analyzes how the productive chain of manioc (Manihot esculenta Crantz is structured. It also explains how indigenous people discuss the intellectual property rights they have on it, and how the geographical indications and the collective trademarks can help to protect traditional knowledge associated to biodiversity.

  5. FEATURES OF WTO DISPUTE SETTLEMENT. THE STANDING OF THE EU

    Directory of Open Access Journals (Sweden)

    Costin Horia Rogoveanu

    2010-09-01

    Full Text Available The WTO has an innovative system of dispute settlement, with the following features: sui-generis, integrated, resolving the disputes according to the WTO agreements, excluding unilateral solutions, interstate system. These features are detailed in the present article. Another level of analysis concerns the standing of the EU in the WTO, in general, and in the Geneva proceedings for dispute settlement, in particular. Generated by the quality of the European Communities statute as an original member of the Organisation, the EU has become one of the main users of the WTO dispute settlement system. One of the main challenges of the WTO dispute settlement mechanism is the implementation of decisions. In view of the cases assessed, while the execution record of the EU is a quite satisfactory one, it is apparent that implementation of decisions in more intricate cases creates difficulties at the Union level.

  6. Arte Popular y Feminismo

    Directory of Open Access Journals (Sweden)

    Eli Bartra

    2000-01-01

    Full Text Available En este artículo se lleva a cabo, en primer lugar, una propuesta metodológica de cómo abordar el estudio del arte popular desde un punto de vista feminista. A continuación se realiza un acercamiento a un ejemplo específico de arte popular mexicano que se halla en estrecha relación con el arte elitista y se puede considerar que se trata de un proceso de sincretismo cultural un tanto sui generis: las friditas de Josefina Aguilar como recreaciones de la obra plástica de Frida Kahlo. Con base en este “close up” se intenta mostrar un posible método para conocer el arte popular contemplando las divisiones sociales por género, etnia y clase.

  7. Professional Sports Club

    Directory of Open Access Journals (Sweden)

    Miodrag Mićović

    2017-01-01

    Full Text Available With regard to the organization of professional clubs, two models can be applied. According to the first model, clubs have the freedom to choose the organizational form (association or commercial company in which sports activities will be carried out. Second model imposes the requirement for the clubs to be organized in the form of commercial company, in one of the corporation forms, provided that it can be also specialized sports form. To the establishment and operation of sports associations, as sui generis commercial companies, apply more specific rules concerning: the conditions for the establishment of clubs; conditions for participation in professional and management bodies; prohibiting multiple ownership of sports clubs, i.e., prohibition of membership; allocation of net profit; control over the work of clubs; state aid (subsidies for the clubs.

  8. Husserl and Stein on the Phenomenology of Empathy: Perception and Explication

    DEFF Research Database (Denmark)

    Jardine, James Alexander

    2015-01-01

    Within the phenomenological tradition, one frequently finds the bold claim that interpersonal understanding is rooted in a sui generis form of intentional experience, most commonly labeled empathy (Einfühlung). The following paper explores this claim, emphasizing its distinctive character......, and examining the phenomenological considerations offered in its defense by two of its main proponents, Edmund Husserl and Edith Stein. After offering in section 2 some preliminary indications of how empathy should be understood, I then turn to some characterizations of its distinctive structure, considering......, in section 3, the Husserlian claim that certain forms of empathy are perceptual in nature, and in section 4, Stein’s insistence that empathetic experience frequently involves explicating the other’s own intentional experiences. Section 5 will conclude by assessing the extent to which their analyses lead...

  9. INSTRUCTIONAL MANUALS OF BOUNDARY‐WORK: PSYCHOLOGY TEXTBOOKS, STUDENT SUBJECTIVITIES, AND DISCIPLINARY HISTORIOGRAPHIES

    Science.gov (United States)

    FLIS, IVAN

    2016-01-01

    This article aims to provide an overview of the historiography of psychology textbooks. In the overview, I identify and describe in detail two strands of writing histories of introductory textbooks of psychology and juxtapose them to provide an integrated historiography of textbooks in psychology. One strand is developed by teachers of psychology—first as a general approach for investigating textbooks in a pedagogical setting, and then later upgraded into a full history of psychology textbooks in America. The other strand follows a more familiar perspective of historians of science and historians of psychology who build on various post‐Kuhnian and post‐Foucauldian perspectives on textbooks. I make an argument for integrating these two views for a more comprehensive historiography of textbooks in psychology, recasting textbooks as objects of research and sources that are interesting sui generis for historians of psychology in their investigations. PMID:27152736

  10. INSTRUCTIONAL MANUALS OF BOUNDARY-WORK: PSYCHOLOGY TEXTBOOKS, STUDENT SUBJECTIVITIES, AND DISCIPLINARY HISTORIOGRAPHIES.

    Science.gov (United States)

    Flis, Ivan

    2016-07-01

    This article aims to provide an overview of the historiography of psychology textbooks. In the overview, I identify and describe in detail two strands of writing histories of introductory textbooks of psychology and juxtapose them to provide an integrated historiography of textbooks in psychology. One strand is developed by teachers of psychology-first as a general approach for investigating textbooks in a pedagogical setting, and then later upgraded into a full history of psychology textbooks in America. The other strand follows a more familiar perspective of historians of science and historians of psychology who build on various post-Kuhnian and post-Foucauldian perspectives on textbooks. I make an argument for integrating these two views for a more comprehensive historiography of textbooks in psychology, recasting textbooks as objects of research and sources that are interesting sui generis for historians of psychology in their investigations. © 2016 The Authors. Journal of the History of the Behavioral Sciences Published by Wiley Periodicals, Inc.

  11. Intellectual property rights and detached human body parts.

    Science.gov (United States)

    Pila, Justine

    2014-01-01

    This paper responds to an invitation by the editors to consider whether the intellectual property (IP) regime suggests an appropriate model for protecting interests in detached human body parts. It begins by outlining the extent of existing IP protection for body parts in Europe, and the relevant strengths and weaknesses of the patent system in that regard. It then considers two further species of IP right of less obvious relevance. The first are the statutory rights of ownership conferred by domestic UK law in respect of employee inventions, and the second are the economic and moral rights recognised by European and international law in respect of authorial works. In the argument made, both of these species of IP right may suggest more appropriate models of sui generis protection for detached human body parts than patent rights because of their capacity better to accommodate the relevant public and private interests in respect of the same.

  12. [Sport, medicine and art: the 'enchanted science' of the body in the works of Thomas Eakins].

    Science.gov (United States)

    Melo, Victor Andrade de; Peres, Fabio de Faria

    2010-03-01

    The article analyzes representations of sport and medicine in the output of the American artist Thomas Eakins, one of the most influential and original in the United States during the transition between the nineteenth and twentieth centuries. It is based on the presupposition that Eakins was able to translate esthetically a plethora of representations related to modernity, including the prelude to intimate relationships and, in an age still sui generis, the practice of sports, health and medicine, transmitted through the idea of a show. This study hopes to be one more contribution to the promotion of what we have called a social archeology of sports, a prospecting of its presence among social networks and webs.

  13. Hasard et nécessité dans la traduction littéraire

    Directory of Open Access Journals (Sweden)

    Irina ALDEA

    2010-11-01

    Full Text Available La relation nécessité-hasard spécifique pour l’acte de création de l’auteur se manifeste avec la même intensité dans l’acte de création du traducteur. L’acte de la traduction littéraire a des règles qui l’individualisent mais qui le maintiennent dans le cadre plus ample des éléments spécifiques à l’acte de création en général. On peut ainsi démontrer, à partir des concepts qui définissent la poïétique que l’acte de traduction est un véritable acte de création, que le traducteur est un auteur sui generis de l’œuvre.

  14. A racionalidade da economia de comunhão e responsabilidade socioambiental: a gestão organizacional influenciada por valores espirituais

    Directory of Open Access Journals (Sweden)

    Valdir Fernandes

    2010-01-01

    Full Text Available This paper discusses the influence of spiritual values in the insertion of socio-environmental dimensions in management processes. A critical analysis of the socio-economic experience named Communion Economy is presented, which highlights the spiritual, ethical and moral values in this economy as inherent motivators of social and environmental considerations. It is a sui generis experience, born within the limits of a religious movement, the Movement of Focolares. It is currently active in 735 companies, mostly small ones, located in several countries, which operate in various economic activities and aggregate a dimension of solidarity to their formal market activity. The considerations presented here reveal a management form based not only on criteria of instrumental rationality (effectiveness, income and performance but also on dimensions of substantive rationality (ethical, aesthetic and moral.

  15. Awakening – Transformation, agency and virtue from three contemporary philosophical inspirations: Bhaskar, Segal and Slote

    Directory of Open Access Journals (Sweden)

    Dudley Schreiber

    2015-10-01

    Full Text Available For some, ‘transformation’ is the new non-reductive and non-normative ‘development’, attracting attention from interdisciplinary array, but of particular theoretical and practical interest to Spirituality scholars. In philosophical context, transformation theory has suffered greatly from ’agency-structure’ dualism and suspension of ontology in body-mind dualism and rationalist virtue controversy. Drawing on the work of Bhaskar, Segal and Slote, a renegotiated and more meaningful sense of transformation emerges from their cumulative analytical and conceptual enrichment. In the complexity of possible relations between self, self-concept and society, lies the traditionally neglected transformative middle of sui-generis human depth. In redress, arguably, Bhaskar’s meta-philosophy accommodates Segal’s experiential depth analysis and Slote’s understanding of empathy and receptivity as valuable insights for ’awakening’ to transformative process.

  16. [Psyche and soma: what can the consultation-liaison psychiatrist contribute?].

    Science.gov (United States)

    Diefenbacher, Albert

    2015-01-01

    In German speaking countries, during the last decades, we can see a growing, though albeit small, integration of psychiatry and psychosomatics into (somatic) medicine. This article outlines the importance of the growing number of elderly patients in medical care as a vantage point for c-l-psychiatrists to play a pro-active role in implementing adequate structures and processes for diagnostics and treatment of this patient group. It is argued that delirium (in dementia) can and should be regarded as a paradigm for a biopsychosocial disorder sui generis. In addition, aspects of the cl-psychiatrists role at two important interfaces of somatic and psychological medicine, i.e. primary care and emergency rooms, are highlighted. Finally, some information about the development of the professionalization of cl-psychiatry in Europe is given.

  17. O duplo legado de Francis Poulenc

    Directory of Open Access Journals (Sweden)

    Joana Resende

    2009-12-01

    Full Text Available Déjàvu,or better,déjà écouté is an appropriate expression to describe an audition of certain works by Francis Poulenc (1899-1963. A sui-generis character in 20th century music, Poulenc has questioned the whole heritage that came before him, French or not, according to the premises of the group he belonged to, Les Six, although not always reaching similar results. His main concern was to make good music, an ideal not far from that of Debussy, “music should humbly seek to please” (Harry Halbreich. This article approaches the use of quotation and other processes of allusion in the works by Poulenc, as seen in his voice and piano mélodies cycle La fraîcheur et le feu, identifying thematic, melodic, and harmonic, origins used coherently in several pieces. and helping to solidly build his musical language.

  18. Organizações no espaço cibernético: estudo comparativo Altavista e Amazon Books

    OpenAIRE

    Cano, Carlos Baldessarini; Becker, João Luiz; Freitas, Henrique Mello Rodrigues de

    2002-01-01

    As organizações passam por mudanças revolucionárias ao incorporar potencialidades surgidas com a convergência tecnológica das indústrias de computação, telecomunicações e realidade virtual. A chamada organização virtual (OV) tem explorado, ao máximo, estas potencialidades, usando, em sua estruturação e operação, inovações tecnológicas que se traduzem, na prática, em características sui generis. Busca-se aqui a verificação da existência destas características peculiares, em organizações virtua...

  19. Organizações no espaço cibernético : estudo comparativo Altavista e Amazon Books

    OpenAIRE

    Cano, Carlos Baldessarini; Becker, Joao Luiz; Freitas, Henrique Mello Rodrigues de

    2002-01-01

    As organizações passam por mudanças revolucionárias ao incorporar potencialidades surgidas com a convergência tecnológica das indústrias de computação, telecomunicações e realidade virtual. A cha-mada organização virtual (OV) tem explorado, ao máximo, estas potencialidades, usando, em sua estru-turação e operação, inovações tecnológicas que se traduzem, na prática, em características sui generis. Busca-se aqui a verificação da existência destas características peculiares, em organizações virt...

  20. Orthotropic Piezoelectricity in 2D Nanocellulose

    Science.gov (United States)

    García, Y.; Ruiz-Blanco, Yasser B.; Marrero-Ponce, Yovani; Sotomayor-Torres, C. M.

    2016-10-01

    The control of electromechanical responses within bonding regions is essential to face frontier challenges in nanotechnologies, such as molecular electronics and biotechnology. Here, we present Iβ-nanocellulose as a potentially new orthotropic 2D piezoelectric crystal. The predicted in-layer piezoelectricity is originated on a sui-generis hydrogen bonds pattern. Upon this fact and by using a combination of ab-initio and ad-hoc models, we introduce a description of electrical profiles along chemical bonds. Such developments lead to obtain a rationale for modelling the extended piezoelectric effect originated within bond scales. The order of magnitude estimated for the 2D Iβ-nanocellulose piezoelectric response, ~pm V-1, ranks this material at the level of currently used piezoelectric energy generators and new artificial 2D designs. Such finding would be crucial for developing alternative materials to drive emerging nanotechnologies.

  1. Topology of surfaces for molecular Stark energy, alignment, and orientation generated by combined permanent and induced electric dipole interactions.

    Science.gov (United States)

    Schmidt, Burkhard; Friedrich, Bretislav

    2014-02-14

    We show that combined permanent and induced electric dipole interactions of linear polar and polarizable molecules with collinear electric fields lead to a sui generis topology of the corresponding Stark energy surfaces and of other observables - such as alignment and orientation cosines - in the plane spanned by the permanent and induced dipole interaction parameters. We find that the loci of the intersections of the surfaces can be traced analytically and that the eigenstates as well as the number of their intersections can be characterized by a single integer index. The value of the index, distinctive for a particular ratio of the interaction parameters, brings out a close kinship with the eigenproperties obtained previously for a class of Stark states via the apparatus of supersymmetric quantum mechanics.

  2. Topology of surfaces for molecular Stark energy, alignment, and orientation generated by combined permanent and induced electric dipole interactions

    Energy Technology Data Exchange (ETDEWEB)

    Schmidt, Burkhard, E-mail: burkhard.schmidt@fu-berlin.de [Institute for Mathematics, Freie Universität Berlin, Arnimallee 6, D-14195 Berlin (Germany); Friedrich, Bretislav, E-mail: brich@fhi-berlin.mpg.de [Fritz-Haber-Institut der Max-Planck-Gesellschaft, Faradayweg 4-6, D-14195 Berlin (Germany)

    2014-02-14

    We show that combined permanent and induced electric dipole interactions of linear polar and polarizable molecules with collinear electric fields lead to a sui generis topology of the corresponding Stark energy surfaces and of other observables – such as alignment and orientation cosines – in the plane spanned by the permanent and induced dipole interaction parameters. We find that the loci of the intersections of the surfaces can be traced analytically and that the eigenstates as well as the number of their intersections can be characterized by a single integer index. The value of the index, distinctive for a particular ratio of the interaction parameters, brings out a close kinship with the eigenproperties obtained previously for a class of Stark states via the apparatus of supersymmetric quantum mechanics.

  3. Sine ars scientia nihil est: Leonardo da Vinci and beyond.

    Science.gov (United States)

    Kickhöfel, Eduardo H P

    2009-01-01

    The aim of this article is to reflect on the relationship between art and science so far as it concerns a symposium on neurosciences. We undertake a historical overview of that relationship, paying particular attention to the sui generis case of Leonardo da Vinci, who very often is regarded as the man who worked on art and science with equal ease. We then explain why his idea of merging these two forms of knowledge failed, considering the clear-cut distinction between art and science in his time. With this clarification, we explore the matter today. We look at Raphael's The Transfiguration, in which the representation of the possessed boy is seen by neuroscientists as indicative of an epileptic seizure. We also look at the ideas of neuroscientists Semir Zeki and Vilayanur Ramachandran, who study particular aspects of brain function and suggest a new merging of art and science.

  4. XBRL Implementation in the Banking Sector in Montenegro

    Directory of Open Access Journals (Sweden)

    Martić Vladan

    2017-05-01

    Full Text Available The EXtensible Business Reporting Language – XBRL appeared in the beginning of the 21st century and it represents one of the most important technological innovations in financial reporting, collecting and exchanging data since the introduction of electronic spreadsheets. However, although the XBRL standard is globally accepted, and it has been proved to be a standard sui generis, the implementation of the standard has not yet become a reality in Montenegro and the region. In this context, the goal of this article is to evaluate a new and, for our conditions unexplored, area from the point of application of modern methods of accounting theory and practice, highlighting both positive and negative aspects. This research is getting prominence having in mind that the EU integration process which Montenegro has already started would affect all areas of socioeconomic life and development, especially in terms of accounting practices harmonization.

  5. Constitution and religiosity of/in the constitutional order of the National Socialist Empire

    Directory of Open Access Journals (Sweden)

    Velez, Pedro

    2017-05-01

    Full Text Available In this article, we will analyse the National Socialist regime as a politico-constitutional reality. We will do it from a new way of looking at politico-constitutional phenomena, interpreting them as registered in a religious grounding. It seeks to show that the National Socialist regime was characterised by having identified the political community – a racially interpreted and raised community to the Absolute – with an empirical historic personality regarded as eminently communitarian. It suggests that the regime constitutes a sui generis case, either in a context of regimes conventionally classified as "right-wing authoritarian and/or totalitarian" or in a larger context of contemporary politics.

  6. A estética da conversão: o ateliê do Engenho de Dentro e a arte concreta carioca (1946-1951 The aesthetics of conversion: the Engenho de Dentro studio and concrete art in Rio de Janeiro (1946-1951

    Directory of Open Access Journals (Sweden)

    Glaucia Villas Bôas

    2008-11-01

    Full Text Available Neste artigo, argumento que na cidade do Rio de Janeiro, no período do pós-guerra, o programa estético modernista orientado para a construção nacional concorre com novo projeto estético fundamentado no universalismo das formas concretas. Tal mudança não ocorre devido às influências recebidas de movimentos de vanguarda estrangeiros ou nacionais, mas de um conjunto de relações e práticas sociais que impõe o reconhecimento de novos valores artísticos. Para tanto, recupero a história do Ateliê do Engenho de Dentro, locus de uma sociabilidade sui generis, que reuniu jovens artistas plásticos, um crítico de arte e artistas internados no Centro Psiquiátrico Nacional Pedro II.In this article I argue that the modernist aesthetic program in post-war Rio de Janeiro, geared towards national construction, competed with a new aesthetic project founded on the universalism of concrete forms. Rather than reflecting the influence of foreign or national avant-garde movements, this change arose from the impact a series of social relations and practices that imposed the recognition of new artistic values. To examine this phenomenon, I reconstruct the history of the Engenho de Dentro Studio, the locus of a sui generis sociability that united young artists, an art critic and artists interned in the Pedro II National Psychiatric Centre.

  7. PERLINDUNGAN HUKUM TERHADAP PENGETAHUAN OBAT-OBATAN TRADISIONAL DALAM REZIM HAK KEKAYAAN INTELEKTUAL (HKI INDONESIA (Studi Pada Masyarakat Tradisional Sasak / LEGAL PROTECTION TOWARD TRADITIONAL MEDICINE KNOWLEDGE IN INDONESIA’S INTELLECTUAL PROPERTY RIGHT REGIME (A Study in The Sasak Traditional Community

    Directory of Open Access Journals (Sweden)

    Dwi Martini

    2017-03-01

    Full Text Available Dalam konteks kekinian Pengetahuan Obat Tradisional (POT masyarakat adat Sasak merupakan aset ekonomi bernilai tinggi mengingat kegunaannya sebagai pengetahuan dasar (milestone dalam penemuan obat modern. Sebagai suatu wujud kemampuan intelektual manusia, POT diatur di bawah rezim HKI-TRIPs, padahal POT memiliki perbedaan karakter yang mencolok dengan HKI. Hal ini memunculkan persoalan dalam hal bentuk POT masyarakat adat Sasak, pengaturan perlindungannya dalam rezim HKI dan pranata hukum ideal untuk mewujudkan perlindungan hukum tersebut. POT Sasak mayoritas ditransmisikan secara lisan, sebagian kecil ada yang tercatat dalam babon (kitab tetamba/oat dan lontar usada. Dalam rezim HKI hanya terdapat pengaturan tidak langsung terhadap POT seperti termuat dalam Undang-Undang Paten dan Perlindungan Varietas Tanaman. Idealnya, terdapat suatu peraturan daerah yang mengatur secara khusus skema perlindungan POT Sasak demi mencegah tindakan misappropriation. Dengan demikian, masih terdapat kekosongan hukum karena belum ada peraturan perundang-undangan sui generis mengenai perlindungan POT. In the modern context, the Traditional Medicine Knowledge (TMK of Sasak community is a valuable economic asset considering its usage as a basic knowledge (milestone in the modern medicine discovery. As a form of human intellectual ability, TMK is regulated under the IPRs-TRIPs regime, whereas TMK have prominent opposite characters with IPRs. This fact raises particular issues in terms of: the form of Sasak community’s TMK, regulation of its protection under the IPRs regime and the ideal legal institution to realize the protection. The majority of Sasak’s TMK are transmitted verbally, a fraction of it was written in babon (book of tetamba/oat and lontar Usada. The IPRs-TRIPs regime only provides indirect regulation toward TMK, as contained in Patent and Plant Variety Protection Law. Ideally, there should be a local Law that particularly regulates protection on

  8. The Suitability of the Remedy of Specific Performance to Breach of a "Player's Contract" with Specific Reference to the Mapoe and Santos cases

    Directory of Open Access Journals (Sweden)

    K Mould

    2011-04-01

    Full Text Available During the 1990s, rugby union formation in the Republic of South Africa developed rapidly from a system of strict amateurism to one of professionalism. Professional participants in the sport received salaries for participation, and rugby became a business like any other. As in all forms of business, rugby had to be regulated moreefficiently than had previously been the case. Tighter regulations were instituted by governing bodies, and ultimately labour legislation became applicable to professional rugby. A professional sportsman or woman participating in a team sport is generallyconsidered an employee. This means that the same principles that govern employees in general should also apply to professional sportsmen and women. The exact nature of the "player's contract", a term generally used to describe the contract of employment between a professional sportsman or sportswoman and his or her employer, deserves closer attention. It has been argued with much merit that the "player's contract", while in essence a contract of employment, possesses certain sui generis characteristics. The first aim of this article is to demonstrate how this statement is in fact a substantial one. If it is concluded that the "player's contract" isin fact a sui generis contract of employment, the most suitable remedy in case of breach of contract must be determined. The second aim of this article is to indicate why the remedy of specific performance, which is generally not granted in cases where the defaulting party has to provide services of a personal nature, is the most suitable remedy in case of breach of "player's contracts". To substantiate this statement, recent applicable case law is investigated and discussed, particularly the recent case of Vrystaat Cheetahs (Edms Beperk v Mapoe. Suggestions are finally offered as to how breach of "player's contracts" should be approached by South African courts in future.

  9. A integração bilateral Brasil-Argentina: tecnologia nuclear e Mercosul

    Directory of Open Access Journals (Sweden)

    Odete Maria de Oliveira

    1998-06-01

    Full Text Available O presente trabalho focaliza o complexo processo de integração bilateral Brasil-Argentina, iniciado formalmente na década de 80, desdobrado em sua dupla abrangência: a cooperação pacífica da tecnologia nuclear; b constituição de um mercado comum. A evolução desse processo conduziu-se através de ciclos fundamentais: primeira etapa, antecedentes de aproximação; segunda etapa, os decisivos instrumentos da ampla integração; terceira etapa, solidificação de duas bases, uma de nível tecnológico nuclear, pactuando um sistema sui generis de salvaguardas e a vigência do Tratado de Tlatelolco (emendado, e, outra, de nível econômico pelo Tratado de Assunção, instituindo o Mercosul.The present work focuses on the complex bilateral integration process involving Brazil and Argentina that began formally during the 80's, and displayed in its double scope: a pacific cooperation on nuclear technology; b constitution of a common market. This process evolution took place in three fundamental circles: the first one, approximation antecedents; the second one, decisive instruments for a large integration; and the third one, the solidification of two basis, one at the nuclear technological level, joining the two countries in the sui generis back up system and the operation of the Tlatelolco Treaty, the other, at the economic level instituting the Mercosur by the Asunción Treaty.

  10. Harmful effect of arbitration dispute settlement EU intra bits to the legal order of the European Union

    Directory of Open Access Journals (Sweden)

    Vukadinović Radovan D.

    2017-01-01

    Full Text Available The issue of interpreting and applying intra BITs, especially in cases where their solutions conflict with EU law or international law, are a reflection of the still unresolved relationship between EU law and international law. Despite the exceptional theoretical significance, the issue of hierarchy is, first and foremost, a political issue. The answer to it defines the legal nature of the EU and the direction of its further development: as a sui generis federal community or a regional community of a confederal type. In the legal theory, the opinion on sui generis and the autonomous legal order of the EU is prevalent. This was also confirmed by the Court of Justice in a series of cases. However, the analyzed decisions of investment arbitrations engaged on the basis of the ISDS mechanism call into question such a position. Arbiters believe that the rights and obligations of the BIT should be interpreted in accordance with the rules of international law and that, in the event of a conflict with EU law, priority should be given to the rules of international public law. For this reason, the European Commission and the Court of Justice only see the existence of ISDS clauses in the BITs as a threat to the preservation of the unity and autonomy of the EU legal order, because their activation is jeopardized by the 'interpretative monopoly' of the Court of Justice entrusted with the decision of previous issues to ensure unity of rights EU. The fact that the question of the effect of the ISDS mechanism is not only of theoretical importance is testified by the fact that the EU negotiations on a new generation of trade and investment agreements are examining proposals to establish a more democratic and transparent multilateral international judicial mechanism in place of the existing mechanism.

  11. O Censo Demográfico de 2010: telefones celulares na construção de podcasts geográficos

    OpenAIRE

    Aléxia Pádua Franco; André Luiz Sabino; Elizabet Rezende de Faria

    2015-01-01

    A sala de aula constitui-se como lugar privilegiado para aplicarmos as novas tecnologias da informação e da comunicação no processo de ensino e/ou aprendizagem. O uso dos telefones celulares na construção de podcasts geográficos permite que os estudantes registrem o conhecimento produzido e pressupõe intensa atividade de pesquisa, de organização de ideias e constante postura reflexiva. Nesse caso específico, o trabalho interdisciplinar com dados estatísticos divulgados pelo Instituto Brasilei...

  12. Mejora de la calidad y disminución de costos de censos y encuestas utilizando computadores de mano (PDA. Una aplicación en Costa Rica

    Directory of Open Access Journals (Sweden)

    Antich-Montero, Daniel

    2007-07-01

    Full Text Available Los datos sobre la población en países en desarrollo se obtienen principalmente de las visitas a los hogares. Ejércitos de entrevistadores recolectan esos datos usando cuestionarios en papel (PAPI, paper and pencil method, pero tal información se infesta de errores no aleatorios. La reciente tecnología de las entrevistas asistidas por computadora (CAPI es un avance importante sobre PAPI en lo que respecta a reducción de errores y los tiempos de espera en la disponibilidad de los datos. Pero la tecnología CAPI ha sido de difícil implementación en los países en desarrollo debido a restricciones ergonómicas, costos y delincuencia asociada a las computadoras portátiles (laptops. Por esto, el Centro Centroamericano de Población (CCP ha desarrollado un sistema CAPI para PDA (Personal Digital Assistant o computadora de mano, que podría revolucionar la forma en que los datos son obtenidos, reduciendo el fraude, errores, tiempos y costos incluso para encuestas complejas. Este artículo reporta las lecciones aprendidas de usar este sistema en el estudio CRELES en Costa Rica.

  13. Mejora de la calidad y disminución de costos de censos y encuestas utilizando computadores de mano (PDA. Una aplicación en Costa Rica

    Directory of Open Access Journals (Sweden)

    Jeisson Hidalgo-Céspedes

    2007-01-01

    Full Text Available Los datos sobre la población en países en desarrollo se obtienen principalmente de las visitas a los hogares. Ejércitos de entrevistadores recolectan esos datos usando cuestionarios en papel (PAPI, paper and pencil method, pero tal información se infesta de errores no aleatorios. La reciente tecnología de las entrevistas asistidas por computadora (CAPI es un avance importante sobre PAPI en lo que respecta a reducción de errores y los tiempos de espera en la disponibilidad de los datos. Pero la tecnología CAPI ha sido de difícil implementación en los países en desarrollo debido a restricciones ergonómicas, costos y delincuencia asociada a las computadoras portátiles (laptops. Por esto, el CCP ha desarrollado un sistema CAPI para PDA (Personal Digital Assistant o computadora de mano, que podría revolucionar la forma en que los datos son obtenidos, reduciendo el fraude, errores, tiempos y costos incluso para encuestas complejas. Este artículo reporta las lecciones aprendidas de usar este sistema en el estudio CRELES en Costa Rica.

  14. O crescimento da libertade religiosa e o declínio da religião tradicional: A propósito do censo 2010

    Directory of Open Access Journals (Sweden)

    Antônio Flávio Pierucci

    2015-06-01

    Full Text Available The article – based on the data of the 2010 census, gathered and interpreted by Flavio Pierucci – deals with the fight to conqueer the followers’ souls in the Brazilian metropolises. The different religions – the Catholic church, the Protestan churces, the cults of African origins, are harsh competitors and every means is allowed to get the conversion. The most relevant datum of the religious scenery – which is extremely dynamic and lively – is the decline of Catholicism, nothwistanding a number of 123 millions declared Catholics in a population of 190 millions. The religious freedom is accompanied by the “secularization”, and consequently the separation between the State and the Church. The disestablishment is the core of the Brazilian religious modernity. The secularization of the state juridical asset is associated with an extraordinary socio-cultural vitality, and, because of the large number of religious operators, missionaries of the new churches, prophets, gurus and magicians, it is extremely difficult to say whether thee Brazialian population is more or less religious now than a century ago.

  15. TENSION AS A HERMENEUTIC OPERATOR FOR POLITICAL RATIONALITY IN HANNAH ARENDT A TENSÃO COMO UM OPERADOR HERMENÊUTICO PARA UMA RACIONALIDADE POLÍTICA EM HANNAH ARENDT

    Directory of Open Access Journals (Sweden)

    Geraldo Adriano E. Pereira

    2008-04-01

    Full Text Available The arendtian research, with its “sui generis” style of topicing the
    politcs and pointing out political’s perplexities, it require our eyes one perception its researcheness logical. We are calling this logical as “tension”, it’s one hermeneutic conception which helps us in the comprehension of his work’s political reasonableness. It has been presented in its many political approaches.a investigação arendtiana, com seu modo sui generis de tematizar a
    política e apontar para as perplexidades do político, exige dos nossos olhos uma percepção de sua lógica investigativa. A esta lógica estamos chamando de ‘tensão’, um operador hermenêutico que nos auxilia na compreensão de uma racionalidade política em sua obra, sendo um presente em suas diversas formas de abordagem da política.

  16. Surgery for Stress Urinary Incontinence

    Science.gov (United States)

    ... Stress Urinary Incontinence Special Procedures What is stress urinary incontinence (SUI)? What causes SUI? What nonsurgical treatment options may help with SUI? What are the surgical treatment options for SUI? What factors are considered when deciding which SUI surgery is ...

  17. A European Res publica

    Directory of Open Access Journals (Sweden)

    Ola Zetterquist

    2011-05-01

    Full Text Available The article analyses the fundamental constitutional enigma of the European Union (EU, namely whether the EU can be considered as a (from its Member States separate and independent constitutional legal order. The EU is often referred to as a legal order sui generis, i.e. of a unique character that defies traditional definitions. More specifically, the notion of an independent and separate EU is at odds with the idea of the sovereign state. The notion of the EU as a legal order sui generis is too much influenced by the models of the sovereign state and sovereignty (in the vein of Thomas Hobbes. The key component in the Hobbesian idea of sovereignty is freedom as non-interference. A sovereign state is consequently a state that is free from, i.e. not interfered with by, external actors like, for example, the EU. Put differently, either the EU is sovereign or the Member States are sovereign. By shifting the perspective to a neo-Roman republican understanding of freedom as non-domination the constitutional picture of the EU will become more nuanced. Res publica is best understood as what citizens hold in common and above their narrow self-interest. According to a republican notion of the constitution the purpose of the law is to eliminate the possibility of arbitrary domination. For that reason, not all interference is to be considered as a restriction of freedom but only those restrictions that cannot be justified according to the res publica. Viewed through the republican prism it can be argued that the EU represents an important advancement in securing freedom as non-domination without implying that the EU must become a state. The fundamental enigma can thus be rephrased as a clash between two diverging concepts of freedom. Whereas the EU will always be at odds with the idea of sovereignty (however framed it will be much easier to reconcile with the republican ideal. DOWNLOAD THIS PAPER FROM SSRN: http://ssrn.com/abstract=1837332

  18. La Iglesia chilena desde 1973 a 1993: De buenossamaritanos, antiguos contrahentes y nuevos aliados. Un análisis politológico

    Directory of Open Access Journals (Sweden)

    M.A. Veit Strassner

    2006-01-01

    Full Text Available El artículo analiza desde un punto de vista politológico el rol de la Iglesia católica chilena en los procesos políticos de los años 1973 a 1993. En un primer paso propone un marco analítico para el estudio de la Iglesia como actor político, poniendo énfasis tanto en el carácter de actor sui generis de la Iglesia como en el contexto sociopolítico donde se desarrolla la acción de la Iglesia. En el segundo paso el artículo muestra el desarrollo paulatino y continuado de la oposición de la Iglesia a la dictadura: Desde la precaución de los comienzos hasta la ayuda sintomática a las víctimas, que fue acompañada por la denuncia de los abusos, la propuesta de alternativas al sistema y la mediación política activa. Así, la Iglesia se convirtió en uno de los actores políticos centrales de la redemocratización. Una vez recuperadas las condiciones democráticas, la Iglesia hizo nuevas alianzas con fuerzas reaccionarias y no democráticas para ver representados sus intereses en determinados campos de la políticaThe article analises, from a political perspective, the role of the Chilean Catholic Church in the political processes of the years 1973-1993. It first offers an analytic frame for the study of the Church as a political actor, emphasizing both its role as sui generis agent, and the sociopolitical context in which the Church operates. Secondly the article shows the gradual but constant development of the Church's opposition to the Dictatorship: From the caution of the beginnings to the overt help to the victims, together with the condemnation of the abuses, the proposal of alternative systems, and active political mediation. Thus the Church turned into one of the key agents of the re-democratization. Once the democratic conditions were recorvered, the Church created new partnerships with reactive and non-democratic forces to see its interests represented in certain fields of the politics

  19. La misión pentecostal escandinava en el Chaco argentino: Etapa formativa: 1914 - 1945 Scandinavian pentecostal mission in the Argentine Chaco: First stage: 1914-1945

    Directory of Open Access Journals (Sweden)

    César Ceriani Cernadas

    2011-01-01

    Full Text Available El trabajo propone una etnografía histórica sobre la misión pentecostal escandinava entre los aborígenes del Chaco Argentino durante su período formativo. Fue el misionero noruego Berger Johnsen (1888-1945 quien se instaló en el pueblo de Embarcación (provincia de Salta en 1914 con el objetivo de organizar una base misionera para el trabajo evangélico. La indagación pretende sumar conocimiento sobre esta corriente olvidada en los estudios sobre evangelización protestante en la región chaqueña tomando en cuenta cuatro ejes: 1. los valores, el imaginario y las prácticas del misionero nórdico; 2. la recepción aborigen del mensaje pentecostal, observada en los dirigentes y la memoria de los habitantes; 3. la coyuntura regional, religiosa, política y económica, que enmarcó estructuralmente el emprendimiento; 4. la realidad cultural sui generis de la misión de Embarcación, conformada por diversos grupos étnicos del corazón chaqueño y establecida en un naciente pueblo criollo.This paper proposes an historical ethnography of the Scandinavian Pentecostal mission among the aboriginal people of the Argentinean Chaco focusing on its former period. In 1914 a Norwegian missionary, Berger Johnsen (1888-1945, established a Pentecostal base in Embarcación (Province of Salta in order to embrace evangelical work. This study tries to add knowledge to this neglected trend in the studies about Protestant evangelism in the Chaco region. This will be done taking into account four topics: 1. the values, representations and practices of the Nordic missionary; 2. the aboriginal appropriation of the Pentecostal message; 3. the regional context, in terms of religion, politics and economy, framing the missionary enterprise; 4. the sui generis cultural reality of Embarcación mission, characterized by the presence of different ethnic groups from the central Chaco and a novel criollo town.

  20. La bioética jurídica islámica como fenómeno jurídico-cultural regional: Tensiones internas y externas en la actualidad producidas en su inserción como derechos humanos

    Directory of Open Access Journals (Sweden)

    Mikunda Franco, Emilio

    2010-10-01

    Full Text Available This paper is the third Part of a Trilogy around the socalled Islamic World. It presents us the “Islamic bioethics” as a legal regional phenomenon instead of an international one, as believed in the West World. It contains a glossary sui generis with specifically Islamic connotations. It means, with similarities and differences far away from the usefully ones in the West World and Spain, so that a big amount of mistakes of interpretation can be avoided, because the aimed words are written in similar way but with very different signification. The paper analyzes the tensions produced in and outside of the Islamic World by associating bioethics and human rights. Such a situation reproduces –in the meaning of the author– another old problem up-to-day not resolved, the so-called “Universal human rights versus regional Islamic human rights”. A selected bibliography in the end contains specifically themes of human rights, Islamic bioethics and constitutionalism, and finally the own contributions of the author.

    El presente trabajo corona la tercera parte de una trilogía en torno al Mundo islámico, tratando la “bioética jurídica islámica” como fenómeno jurídico regional frente a la occidental, autodenominada universal. (Anteriores partes fueron “Derechos humanos”, y “constitucionalismo” islámicos. Aporta un glosario terminológico islámico sui generis con connotaciones específicas, similitudes y diferencias frente a la occidental al uso en España, en evitación de posibles confusiones o lapsos debidos al uso de términos de idéntico significante y homólogo significado. Analiza tensiones de todo tipo producidas al asociar “bioética y derechos humanos en el mundo islámico”. Condensa en diez tesis finales la polémica y problemática resultante, que reproduce –según su autor– análoga situación de otrora entre “Derechos humanos universales versus Derechos humanos islámicos regionales”. La

  1. Fin de guerra y reinserción de excombatientes. La Legitimidad del Programa de Atención Humanitaria al Desmovilizado

    Directory of Open Access Journals (Sweden)

    Daniel Castaño Zapata

    2015-08-01

    Full Text Available Partiendo de la hipótesis de que el Programa de Atención Humanitaria al Desmovilizado, implementado desde 2003 en el proceso de reinserción de excombatientes paramilitares en Colombia, es susceptible de ser analizado como una Política Social sui géneris, cuyo fin no es intervenir los procesos de distribución de la riqueza, sino que busca pacificar a la sociedad, este artículo analiza y describe el proceso de legitimación social del PAHD. Se busca señalar que la recepción social de los programas de reinserción de excombatientes es determinante en las características del posconflicto, pues a partir de esta se resuelven tensiones constitutivas como la integración social y la definición del monopolio de la violencia. La exposición reconstruye testimonios y argumentos que componen las formas de reconocimiento e impugnación al PAHD. Based on the hypothesis that the Humanitarian Assistance Program for the Demobilized, implemented since 2003 during the reinsertion process of former paramilitary combatants in Colombia, can be analyzed as a sui generis Social Policy which purpose is not to interfere with the richness distribution processes but to pacify society, this article is intended to analyze and describe the social legitimation process of the HAPD. The article makes emphasis on the fact that social acceptance of the reinsertion programs for former combatants is decisive for the specific features of the post-conflict since social acceptance is the basis for solving tensions such as social integration and definition of the violence monopoly. The article reconstructs testimonies and arguments that comprise recognition and challenge of the HAPD

  2. Sui pronomi personali di prima e seconda persona plurale in italiano

    Directory of Open Access Journals (Sweden)

    Rosanna Sornicola

    1991-12-01

    Full Text Available Nella sua fondamentale Grammatica storica dell'italiano Tekavčić accoglie come spiegazione delle forme pronominali italiane noi, voi una trafila che parte dalle forme nominativo-accusative latine NŌS, VŌS ed esibisce un successivo sviluppo fonetico caratteristico dei monosillabi con uscita in -s, ovvero /si-> /y/. La tesi di una evoluzione di /s/ in posizione finale in /y/, nell'area che include toscano, dialetti italiani centro-meridionali e balcano-romanzo (veglioto, romeno era già stata avanzata da Reichenkron (1939. In tale area sia parole monosillabiche che polisillabiche presenterebbero questo sviluppo. Ad esemplificare le prime sono chiamate in causa forme come rom. e it. dai < DAS, rom. e it. stai < STAS, rom. e it. poi < POS ( < POST, it. centr. e merid. crai < CRAS, it. merid. ccuy e it. più < PLUS, rom. e it. merid. trei < TRES, oltre per l'appunto, alle forme pronominali rom., vegl. e it. noi < NOS e voi < VOS. Nei polisillabi questo sviluppo si mostrerebbe con minore evidenza, perché qui /y / si sarebbe fusa con la vocale atona precedente, o sarebbe caduta: si pensi all'it. ant. cante (it. mod canti, tosc. sett. canta, rom. cânţi < CANTAS; it. vedi, rom. vezi < VIDES, e così via.

  3. Esplosioni di icone. Street art e iconoclastia performativa sui monumenti socialisti dell'Europa orientale

    Directory of Open Access Journals (Sweden)

    Mario Panico

    2017-09-01

    Full Text Available The aim of this paper is to consider the actions of street art and urban recolouring on the monuments of communist dictatorship, which have not been demolished after the fall of the Berlin Wall in 1989. Such rewriting actions have converted these monuments in spaces of artistic disputes in stark contrast with the memory conveyed by the communist regime. My goal is to understand the semiotic logic that characterizes the art of protest on the dictatorship monuments. In addition, with the analysis of three specific monuments I will investigate the relationship between imaginary and iconoclasm with the purpose of defining a new form of "attack on image" which is not only aimed at the destruction but also at the creation of new imaginaries.

  4. Un Woyzeck sui fronti del Novecento. «Eiche und Angora» di Martin Walser

    Directory of Open Access Journals (Sweden)

    Maurizio Pirro

    2014-10-01

    Full Text Available Martin Walser’s play Eiche und Angora (1962 deals with the persistence of totalitarianism-oriented mental structures after the Second World War and the collapse of the Nazi regime. Walser represents political history as strongly opposed to the private sphere of ordinary people. Adjustment to the post-war situation of the Bundesrepublik and continuity with the historical past are seen by Walser as equivalent forms of self-defense against the inhuman attitude of political power.

  5. #JeSuisCharlie: Towards a Multi-Method Study of Hybrid Media Events

    Directory of Open Access Journals (Sweden)

    Johanna Sumiala

    2016-10-01

    Full Text Available This article suggests a new methodological model for the study of hybrid media events with global appeal. This model, developed in the project on the 2015 Charlie Hebdo attacks in Paris, was created specifically for researching digital media—and in particular, Twitter. The article is structured as follows. Firstly, the methodological scope is discussed against the theoretical context, e.g. the theory of media events. In the theoretical discussion, special emphasis is given to i disruptive, upsetting, or disintegrative media events and hybrid media events and ii the conditions of today’s heterogeneous and globalised media communication landscape. Secondly, the article introduces a multi-method approach developed for the analysis of hybrid media events. In this model, computational social science—namely, automated content analysis (ACA and social network analytics (SNA—are combined with a qualitative approach—specifically, digital ethnography. The article outlines three key phases for research in which the interplay between quantitative and qualitative approaches is played out. In the first phase, preliminary digital ethnography is applied to provide the outline of the event. In the second phase, quantitative social network analytics are applied to construct the digital field for research. In this phase, it is necessary to map a what is circulating on the websites and b where this circulation takes place. The third and final phase applies a qualitative approach and digital ethnography to provide a more nuanced, in-depth interpretation of what (substance/content is circulating and how this material connects with the ‘where’ in the digital landscape, hence constituting links and connections in the hybrid media landscape. In conclusion, the article reflects on how this multi-method approach contributes to understanding the workings of today’s hybrid media events: how they create and maintain symbolic battles over certain imagined constructs of social imaginaries of solidarity, belonging, contestation, and exclusion, a topic of core value for the theory of media events.

  6. Italia: La chiusura della Casa dell’Emigrante come spunto riflessione sui rientri degli emigrati italiani

    OpenAIRE

    Aluffi Pentini, Anna; Olivieri, Fabio

    2009-01-01

    In the literature on migration, as well as in social policies regarding this phenomenon, the situation of returning emigrants receives scant attention. This essay establishes an intricate connection between attitudes and policies that prevail in a country regarding emigration and those concerning immigration. The case of Italy provides a prime example for this as it once was a classical country of emigration, only to turn, in recent decades, into a country that appears highly attractive (and ...

  7. Tra cielo e vulcano: sui confini tematici e stilistici in Stromboli di Roberto Rossellini

    Directory of Open Access Journals (Sweden)

    Vincenzo Maggitti

    2011-05-01

    Full Text Available This article is focused on a film by Roberto Rossellini, Stromboli (1949 whose title comes from the island, off the northwest coasts of Sicily, on which the film is set. Given the thematic relevance of borders in the post‐war drama of a foreign refugee who moves to the unknown island with a husband met through the barbed wire of the camp, the article analyzes to what extent this theme is rebounded in the unstable genre of the film. In Stromboli a coexistence of tropoi belonging to different traditions of melodrama can be spotted, tracing back to the theatrical origins of the genre, as detailed in Jean‐Marie Tommaseau´s recent study. But one must take into account, nevertheless, the documentary‐drive that inspires the film, linking it to Rossellini´s approach to the new unmapped reality of the Italian landscape, recently discussed by Angelo Restivo in his essay on Italian cinema during the economic boom. The interplay of these theoretical references in Stromboli can bring to the fore the theme of space and time borders as more relevant than the traditional critical filing of the film as a study of loneliness, together with the other four features made with actress Ingrid Bergman. The compounded analysis results in a melodramatic re‐vision of the film, that can entail the ending shift towards the holy and the sacred often dismissed as improper. The article comments on the film conveying melodrama into a field supposedly outside of its genre concerns.

  8. Italia: La chiusura della Casa dell’Emigrante come spunto riflessione sui rientri degli emigrati italiani

    Directory of Open Access Journals (Sweden)

    2009-01-01

    Full Text Available In the literature on migration, as well as in social policies regarding this phenomenon, the situation of returning emigrants receives scant attention. This essay establishes an intricate connection between attitudes and policies that prevail in a country regarding emigration and those concerning immigration. The case of Italy provides a prime example for this as it once was a classical country of emigration, only to turn, in recent decades, into a country that appears highly attractive (and relatively accessible to immigrants. The essay traces the pervasive ambiguity that characterizes this country’s attitudes towards emigration from the beginning of mass emigration shortly after the unification of Italy in 1868 to the emigration policies of the fascist regime of Mussolini and the post-World War II waves of emigration right through to the corresponding ambiguity concerning the status of immigrants in contemporary society, including the indifferent treatment of returning Italian emigrants who constitute a considerable numerical phenomenon. These reflections take their origin from the impending closure of a reception centre in Lazio, the Casa dell’Emigrante near Sant’Elia Fiumerapido, Province of Frosinone, ostensibly for financial reasons. This centre had been the only one of its kind in the whole of Italy dealing officially with the needs of repatriated Italians. It had assisted returning emigrants both with practical matters, such as negotiating the labyrinth of Italian bureaucracy , and with psychological implications of a return, which are often considerable given the time lag of experiences with current social realities and the frequently unrealistic expectations associated with the return. Questions of identity become highly acute in those circumstances. The threatened closure of the centre illustrates the unwillingness of the state to face up to the factual prevalence of migratory experiences in the country as a whole and as a core element of national history, experiences of migration in both directions. The statistics speak for themselves: of the 4.660.427 persons who left Italy between 1880 and 1950, 2.322.451 have returned, almost exactly 50%. To those have to be added 3.628.430 returnees of the 5.109.860 emigrants who left Italy between the end of World War II and 1976 for Europe alone. Attitudes towards people leaving changed ostensibly over time. In the first two decades after Unification parliament on the one hand wanted to show some concern over the fate of its citizens, not wanting to abandon those newly created citizens entirely to their own destiny, while on the other portraying their decisions to emigrate as expressions of individual liberty and responsibility and not necessitated by want and poverty. Emigrants had to prove, paradoxically that they had the requisite means to emigrate when in fact poverty was largely driving them to emigrate. To admit that publicly would have amounted to admission of economic and political failure made evident through emigration. In contrast to that Mussolini’s emigration policies not only enforced large population movements within the territory of Italy to balance unemployment between regions and particularly between North and South, but also declared it citizen’s duty to be ready to move also to the colonies, thereby ‘turning emigration as a sign of social crisis into a sign of national strength and the success of the country’s political agenda’ (Gaspari 2001, p. 34. The duplicity continued even after World War II when secret deals were done with the USA to allow a continuous flow of Italian immigrants and EU membership obviously further facilitated the departure of unemployed, impoverished Italians. With the growing prosperity of Italy the reversal of the direction of migration became more obvious. On the basis of empirical research conducted by one of the author on returning emigrants four types of motives for returning can be distinguished: 1.Return as a result of failure – particularly the emigrants who left during the 1950-1970 period usually had no linguistic preparation, and in any case the gap between the spoken and the written language is enormous with the latter often being insurmountable. This gives rise to nostalgic sentiments which motivates a return into an environment where language is familiar 2.Return as a means of preserving an identity – the life of emigrants often takes place within ghetto-like conditions where familiarity is being reproduced but under restricted conditions and hence not entirely authentic. The necessity for saving money permits only a partial entry into the host society and at the same time any accumulating savings add to the desire to return home where life can be lived fully again – or so it seems. 3.Return of investment – the impossibility to become fully part of another society often motivates migrants to accumulate not so much material wealth but new experiences and competences which they then aim to reinvest in their home country. 4.Return to retire – for many emigrants returning home becomes acute once they leave a productive occupation and feelings of estrangement build up, in conjunction with the efforts of having invested in building a house back home. All those motives are associated with a variety of difficulties on the actual return home because, above all, time in relation to the country of origin has been suspended for the emigrant and the encounter with the reality of that country reveals constant discrepancies and requires constant readjustment. This is where the need for assistance to returning emigrants arises. The fact that such an important centre of assistance has been closed is further confirmation of the still prevailing politics of ambiguity which nominally demand integration from nationals and non-nationals alike but deny the means of achieving this. Citizenship is not a natural result of nationality but requires the means for active participation in society. Furthermore, the experiences of returning immigrants provide important cues for the double ambivalence in which immigrants to Italy live between the demands made on them to integrate, the simultaneous threats of repatriation and the alienation from the immigrants’ home country which grows inexorably during the absence. The state can only regain its credibility by putting an end to this ambiguity and provide to returning emigrants, and immigrants alike, the means of reconstructing strong communal identities.

  9. Analisi sui pigmenti di ceramica neolitica tramite tecniche Raman e LIBS

    Directory of Open Access Journals (Sweden)

    Cristina Fabbri

    2007-04-01

    Full Text Available I dati presentati in questo breve contributo provengono da uno studio condotto in collaborazione dal Dipartimento di Scienze Archeologiche dell’Università di Pisa e dall’Istituto per i Processi Chimico-Fisici di Fisica molecolare del CNR di Pisa. Le metodologie applicate sono basate sull’analisi integrata delle tecniche spettroscopiche Raman e LIBS già ampiamente utilizzate nel campo dei Beni Culturali; i metodi sono infatti essenzialmente non distruttivi, non richiedono di un pre trattamento del campione e l’acquisizione delle misure è immediata.

  10. Doppia imposizione sui redditi transfrontalieri tra ordinamenti tributari e mercato europeo.

    OpenAIRE

    Bottazzi, Cristiana

    2012-01-01

    La ricerca affronta in modo unitario e nell’ottica europea i multiformi fenomeni della doppia imposizione economica e giuridica, assumendo come paradigma iniziale la tassazione dei dividendi cross-border. Definito lo statuto giuridico della doppia imposizione, se ne motiva la contrarietà all’ordinamento europeo e si indagano gli strumenti comunitari per raggiungere l’obiettivo europeo della sua eliminazione. In assenza di un’armonizzazione positiva, il risultato sostanziale viene raggiunto...

  11. Sui diritti dell’infanzia e la libertà che comincia

    Directory of Open Access Journals (Sweden)

    Mariangela Caporale

    2014-06-01

    Full Text Available The present article deals with children’s rights. It focuses on the problematic aspect regarding all the documents – included the Convention on the Rights of the Child - that only support adults’ point of view about life and world without considering the peculiarity of the reason and freedom of childhood, that is its autonomy.

  12. Fluxus (fayd). Indagine sui fondamenti della Metafisica e della Fisica di Avicenna

    NARCIS (Netherlands)

    Lizzini, O.L.

    2011-01-01

    La teoria della causalità di Avicenna (Ibn Sīnā, m. 1037) è dominata dal concetto neoplatonico di flusso emanativo (in arabo fayḍ). Tutti i piani del sistema avicenniano ne sono interessati: quello metafisico, quello naturale, e quello intellettuale e quindi gnoseologico ed etico. Sulla base di

  13. Analisi dei cicli del mercato immobiliare: un applicazione sui dati italiani

    Directory of Open Access Journals (Sweden)

    Maurizio Festa!

    2012-06-01

    Full Text Available Utilizzando i dati italiani, sulla base dell'approccio teorico del ciclo "a nido d'ape" proposto da Janssen, Kruijt e Needham nel 1994, questo articolo propone un ulteriore contributo allo studio delle dinamiche del mercato immobiliare italiano. Utilizzando alcuni indicatori del mercato, si presentano i grafici del ciclo a nido d'ape per tutte le regioni e per le principali città. Tecniche di cluster analysis sono utilizzate per raggruppare le regioni che mostrano andamenti simili. L'obiettivo finale dell'analisi è quindi quello di esaminare il territorio nazionale in termini di sviluppo ed evoluzione del mercato delle abitazioni.

  14. Tecnologie didattiche e beni culturali: una rassegna sui musei italiani nell’era del web

    Directory of Open Access Journals (Sweden)

    Simona Ottaviano

    2012-12-01

    Full Text Available An overview of websites of Italian museums, archives and libraries that are providing users with information and learning resources with particular attention to the young and people with disabilities.

  15. Arsenic levels in thermal waters; Indagine sui contenuti di arsenico in acque termali

    Energy Technology Data Exchange (ETDEWEB)

    Conti, Marcelo Enrique [Rome, Univ. La Sapienza (Italy). Ist. di Merceologia

    1997-10-01

    The present article reports the results of a study on the determination of As levels in thermal waters by Atomic Absorption Spectrometry. Samples of thermal waters have been collected from 4 thermal resorts located in the central Italy. AA Spectrometry assays have been carried out on 140 samples, collected seasonly for one year from 14 thermal springs. The results of the present study have revealed As levels lying below the detection limits of the AA technique for 12, 1% of the total number of assayed samples. The remaining 87,9% of samples gave a range of As concentration values comprised between 0,20 and 0,68 mg/l, that is in agreement with the range of average values reported for other italian thermal waters.

  16. TVT-O vs. TVT for the treatment of SUI: a non-inferiority study.

    Science.gov (United States)

    Yang, Xiang; Jiang, Min; Chen, Xinliang; Tong, Xiaowen; Li, Huaifang; Qiu, Jin; Shao, Lingyun

    2012-01-01

    This study aimed to prospectively compare, in terms of efficacy and safety, the tension-free vaginal tape (TVT) and the transobturator vaginal tape inside-out (TVT-O) procedure for stress urinary incontinence. A cough stress test was applied to the objective outcomes, while urinary incontinence-specific quality of life questionnaire was applied to the subjective outcomes. A test for non-inferiority was carried out for detecting the success rate between the two groups. The objective success rates were found to be 95.4% (62/65) in the TVT group and 96.4% (108/112) in the TVT-O group. No significant difference was found between these two groups in the success rate by non-inferiority test (P 0.05). In the study, the TVT-O procedure could be defined to be identical to the TVT approach in success rate by non-inferiority test.

  17. Considerazioni sui nuovi servizi di conversione di coordinate del Geoportale Nazionale

    Directory of Open Access Journals (Sweden)

    Roberto Rossi

    2013-04-01

    questo passaggio, i servizi di conversione del Geoportale Nazionale siano un preziosissimo strumento a disposizione di tutti... Publication of conversion systems of coordinates on the National Geoportal has attracted positive comments in general, but also some criticism by users.The author hopes that transformation grids owned by Italian Military Geographic Institute will be soon available as Open Data. Anyway he believes that, meanwhile, conversion and transformation services offered by National Geoportal are precious tools accessible to everyone.

  18. Considerazioni sui nuovi servizi di conversione di coordinate del Geoportale Nazionale

    Directory of Open Access Journals (Sweden)

    Roberto Rossi

    2013-04-01

    Full Text Available La pubblicazione dei sistemi di conversione di coordinate sul Geoportale Nazionale ha suscitato commenti in generale positivi, ma anche qualche critica da parte degli utenti. Chiscrive auspica che i grigliati IGM, in un prossimo futuro, entrino a far parte del patrimonio dei dati pubblici e aperti del nostro paese. Ritiene però che, in attesa che i tempi siano maturi perquesto passaggio, i servizi di conversione del Geoportale Nazionale siano un preziosissimo strumento a disposizione di tutti...Publication of conversion systems of coordinates on the National Geoportal has attracted positive comments in general, but also some criticism by users.The author hopes that transformation grids owned by Italian Military Geographic Institute will be soon available as Open Data. Anyway he believes that, meanwhile, conversion and transformation services offered by National Geoportal are precious tools accessible to everyone. 

  19. Characterization of Streptococcus suis serotype 7 isolates from diseased pigs in Denmark

    DEFF Research Database (Denmark)

    Tian, Y.; Aarestrup, Frank Møller; Lu, C.P.

    2004-01-01

    to erythromycin (41%), tetracycline (24%) and streptomycin (28%) was observed. Furthermore, almost all isolates (101) were resistant to sulphamethoxazol. Most isolates were susceptible to ceftiofur, chloramphenicol, florfenicol, penicillin, ciprofloxacin, trimethoprim and trimethoprim + sulphonamides. The tet...

  20. Modelling of phase change materials in the Toronto SUI net zero energy house using TRNSYS

    Energy Technology Data Exchange (ETDEWEB)

    Siddiqui, O.; Fung, A.; Zhang, D. [Ryerson Polytechnic Univ., Toronto, ON (Canada). Dept. of Mechanical and Industrial Engineering

    2008-08-15

    In the context of building applications, phase change materials (PCM), can be defined as any heat storage material that can absorb a large amount of thermal energy while undergoing a change in phase, such as from a solid to a liquid phase. The incorporation of PCM into the building envelope can enhance occupant comfort through the reduction of indoor temperature fluctuations. It has also been shown to cause a decrease in the overall energy consumption associated with the heating and cooling of buildings. This paper extended the analysis of the impact of using PCM, which has traditionally focused on homes of ordinary construction, to incorporate low to zero energy homes using a model of the Toronto net zero energy house developed in TRNSYS. The paper provided a description of the TRNSYS model/methodology, with reference to the wall layer used in the net zero energy house, and model of the layout of the net zero energy house in TRYNSYS. The TRYNSYS/type 204 PCM component was also presented along with the simulation results in terms of the temperature profile of the third floor of the net zero energy house on a typical winter day with varying PCM concentrations; the temperature profile of the third floor of the net zero energy house on a typical summer day with varying PCM concentrations; yearly heating/cooling load requirements of the net zero energy house for a variety of thermal mass used; temperature profile of the third floor of the net zero energy house on a typical summer day when PCM and concrete slab was used; yearly temperature profile of the third floor of the net zero energy house, illustrating the impact of using PCM; and the yearly heating/cooling load of the net zero energy house as the concentration of PCM was varied. It was concluded that the use of building integrated PCM can reduce temperature fluctuations considerably in the summer but only slightly in the winter. 16 refs., 1 tab., 8 figs.

  1. Il difficile percorso verso una legge organica sui culti in Venezuela

    Directory of Open Access Journals (Sweden)

    Antonio Ingoglia

    2011-03-01

    Full Text Available SOMMARIO: 1. Presupposti storico-giuridici - 2. Contenuti generali del progetto di Legge Organica “De Religiòn y Culto” all’esame dell’Assemblea Nazionale - 3. Il dibattito politico e i rilievi dei culti minoritari - 4. La posizione dell’episcopato cattolico e la questione relativa all’estensione della legislazione organica alla Chiesa Cattolica - 5. Perdurante tendenza giurisdizionalista del diritto ecclesiastico venezuelano. Due esempi: le disposizioni della “Ley Organica de Immigraciòn” e del disegno di “Ley Organica de Educaciòn” circa le funzioni ispettive e di controllo dello Stato sull’attività dei culti.

  2. Temperature dependent embryonic development of Trichuris suis eggs in a medicinal raw material

    DEFF Research Database (Denmark)

    Vejzagic, Nermina; Kringel, Helene; Bruun, Johan Musaeus

    2016-01-01

    in Göttingen minipigs.Both male and female pigs were used to evaluate eventual gender specific infectivity. Storage at 30 °C up to 14 weeks and subsequent embryonation for 14 weeks at 25 °C did not significantly reduce the overall larval establishment in minipigs, as compared to storage at 5 °C and subsequent...... analysis (OvaSpec), and an egg hatching assay prior to the final testing in minipigs (Trial 1). These methods showed that the development started earlier at higher temperatures, but the long-term storage at higher temperature affected the egg development. The present study further documents tolerance...

  3. SUI ja UUI vaheline seos on keerukas / tõlkinud Kallle Klein

    Index Scriptorium Estoniae

    2014-01-01

    Meditsiiniuuringuist, kus pole õnnestund kindlaks teha ühtegi modifitseeritavat riskifaktorit, mis oleks seotud pakilise uriinipidamatusega naiste hulgas, kellele tehakse operatsioon stressikontinentsi tõttu

  4. Potential use of G-CSF for protection against Streptococcus suis infection in swine

    Science.gov (United States)

    The use of immunomodulators is a promising alternative to the use of antibiotics for therapeutic, prophylactic, and metaphylactic use to prevent and combat infectious disease. We developed a replication-defective adenovirus vector that expresses porcine granulocyte colony-stimulating factor (G-CSF) ...

  5. Expressing epistemic stance in University lectures and TED talks: a contrastive corpu-based analysis

    Directory of Open Access Journals (Sweden)

    Giuditta Caliendo

    2014-07-01

    ruolo fondamentale nei processi di ricontestualizzazione e riconcettualizzazione del sapere specialistico. Oltre a riadattare i contenuti per un pubblico di non addetti ai lavori, attraverso i nuovi mezzi di comunicazione gli esperti hanno la possibilità di rimodellare la propria immagine in base ai propri scopi comunicativi. Questo articolo si incentra sui TED talk, interventi a convegno (TED conferences trasmessi in tutto il mondo attraverso la rete in cui esperti di diverse discipline conferiscono su vari temi (ad es. scienza, tecnologia, architettura, questioni globali. I TED talk condividono molte caratteristiche con una serie di generi ‘satellite’, come i documentari, le presentazioni accademiche, gli articoli scientifici e le lezioni universitarie. Queste ultime rappresentano probabilmente il genere che maggiormente si avvicina ai TED talk. Sia nelle lezioni universitarie sia nei TED talk, infatti, l’esperto si rivolge ad un pubblico di non esperti (o semi-esperti avvalendosi di varie modalità semiotiche (ad es. parlato, supporti audio, supporti visivi in quello che si rivela essere un evento comunicativo essenzialmente monologico. Prestando particolare attenzione a un processo di ricontestualizzazione del discorso accademico, questo contributo si propone di analizzare se e fino a che punto il discorso accademico subisce un cambiamento nel momento in cui il parlante si sposta dall’aula universitaria al palco di TED. Questo studio si basa su un’analisi contrastiva: un corpus di TED talk tenuti da accademici viene messo a confronto con un corpus di lezioni universitarie ricavato dal Michigan Corpus of Academic Spoken English (MICASE. Per osservare differenze e analogie fra i due generi ci si avvale di un’analisi sia quantitativa sia qualitativa.   Parole chiave: Lingue per Scopi Speciali, Divulgazione, Generi multimediali, Evidentiality, Epistemic stance.

  6. La industria del petróleo en México, 1911-1938: del auge exportador al abastecimiento del mercado interno. Una aproximación a su estudio

    Directory of Open Access Journals (Sweden)

    Luz María Uhthoff López

    2010-06-01

    Full Text Available En este trabajo se examina el crecimiento de la demanda interna del petróleo y sus derivados durante el periodo comprendido entre 1925 y 1938. En este lapso, México transitó de una industria eminentemente exportadora de petróleo crudo a la puesta en marcha de una refinadora, con la que el consumo interno de petróleo cobraría mayor relevancia. En este aumento del consumo fue importante la política económica de los gobiernos posrevolucionarios que, a través de estímulos fiscales, el establecimiento de organismos semioficiales y el control de precios favorecieron la expansión de este mercado.This paper examines the raising of oil products in domestic consumption along the 1925-1938 period. From just having mainly crude oil export industry, Mexico shifted towards a refining oriented one dealing with the internal oil market that would became so gready important. Finally, it is pointed out how the post revolutionary governments played a main roll on supporting the sustained growing of oil internal consumption through post revolutionary economical policies based on tax exemptions, sui generis social organizations and, last but not least, the oil price control established deliberately to strength the home expanding market.

  7. The genesis of collective health in Brazil.

    Science.gov (United States)

    Vieira-da-Silva, Ligia Maria; Pinell, Patrice

    2014-03-01

    During the 1970s in Brazil a social space directed towards health problems on the population level, called collective health, was created and institutionalised. To what extent did this Brazilian invention correspond to a specific socio-historical practice? The works published on this topic have considered social medicine as a homogeneous phenomenon without empirically studying the specificities of national experiences. To bridge this gap, a historical study on the genesis of collective health in Brazil was carried out based on Bourdieu's field theory. The interaction between the paths of the founders and the conditions of historical possibilities were researched through documentary and bibliographical sources, as well as through in-depth interviews of the founders. This social space originated from a meeting of agents with different social backgrounds but who interconnected, creating a structure that was independent of each agent considered individually. One of the components of this establishment was the joining of theoretical production and the implementation of health reforms that resulted in the organisation of a universal health system. This study attempts to show how the international political situation and the contradictions of the national crisis created a universe of possibilities, allowing for the genesis of this sui generis space in Brazil. © 2013 The Authors. Sociology of Health & Illness © 2013 Foundation for the Sociology of Health & Illness/John Wiley & Sons Ltd.

  8. Grense tussen kerk en staat vir die reëling van die predikantsdiens: gesien vanuit twee kerkregtradisies

    Directory of Open Access Journals (Sweden)

    J. Smit

    2009-07-01

    Full Text Available Boundaries for church and state regarding the regulation of the ministry of the Word: seen from two church polity traditions This article focuses on the boundaries for church and state with regard to the regulation of the ministry of the Word – seen from two independent church polity traditions. Until now the South African courts have not given an indication of how these boun- daries should be understood or how these apply within the South African context. The purpose of this article is not to make a judicial comparison. The purpose is to show that two indepen- dent church polity traditions, namely the German and the reformed, developed general principles for the boundaries of church and state with regard to the regulation of a minister’s position in law. In both traditions church polity is regarded sui generis, and recognises the church’s right to regulate a minister of religion’s position independently from the state. In conclusion it is found that the boundaries between church and state for the ministry of the Word are primarily determined by the principle that church and state should not in any way influence or interfere with one another's separate mandates or duties. The ministry of the Word is the responsibility of the church and not the state. Therefore the state does not have the competency to regulate the ministry of the Word.

  9. Das Paarinterview in der soziologischen Paarforschung. Method(ologische und forschungspraktische Überlegungen

    Directory of Open Access Journals (Sweden)

    Christine Wimbauer

    2017-03-01

    Full Text Available Paarinterviews erlauben die Erfassung von Interaktionen, Aushandlungen, Performances und Präsentationen des Paares und als Paar in situ. Sie liefern Einblicke in die konkrete Paarpraxis und in die Darstellung der Praxis im Interview als doing couple, aber auch als doing gender, doing family, doing recognition oder doing inequality. Mit dem Blick auf Individuen in Paarbeziehungen mittels Paarinterviews können Macht- und Ungleichheitsverhältnisse im Paar sowie die Prozesshaftigkeit und Dynamik des Sozialen ausschnitthaft beobachtet werden. Paarinterviews werden in der nichtstandardisierten Sozialforschung zunehmend, jedoch weitaus seltener als Einzelinterviews verwendet. Bisher gibt es im deutschsprachigen Raum kaum systematische methodische und methodologische Überlegungen zum gemeinsamen Paarinterview. Basierend auf der Fassung von Paarbeziehungen als Realität sui generis und als eigenständigem Analysegegenstand zeigen wir – verortet im interpretativen Paradigma – Erkenntnisinteressen, Anwendungsbereiche und Stärken von Paarinterviews für die soziologische Paarforschung auf. Zudem präsentieren wir neben ausgewählten soziologischen Paarforschungen methodische Aspekte der Durchführung und Anwendung, method(ologische Grenzen von und offene method(ologische Fragen zum Paarinterview (nicht nur in der soziologischen Paarforschung. URN: http://nbn-resolving.de/urn:nbn:de:0114-fqs170243

  10. DEUS É MAIS: A SUPREMACIA DA FÉ EVANGÉLICA NA ÓTICA DOS ATLETAS DE CRISTO

    Directory of Open Access Journals (Sweden)

    Reinaldo Olécio Aguiar1

    2011-01-01

    Full Text Available A seleção de futebol do Brasil que conquistou o título de campeã mundial, na Copa do Mundo de 1994, nos Estados Unidos, contava com seis atletas “evangélicos”. Este grupo reduzido, que se considerava “escolhido”, coordenado por pessoas ligadas ao grupo Atletas de Cristo (ADC, encontrou uma explicação sui generis para a conquista do título: Deus. Analisando a trajetória de cada um dos seis atletas no período da competição, Alex Dias Ribeiro, diretor dos ADC, apresentou não só uma versão religiosa de um evento esportivo, como a suposta superioridade da “fé evangélica” em relação aos outros produtos religiosos. Trabalhamos neste artigo com a hipótese de que esta idéia de superioridade decorre da herança evangelical que separa convertidos e não-convertidos, com os primeiros sendo abençoados por Deus, além da mudança de linguagem interpretadora do mundo do convertido, realizada agora a partir de sua experiência religiosa. A conseqüência é um tipo de humildade evangélica que atribui as grandes ações a Deus, tanto na vida cotidiana quanto no esporte.

  11. The Soviet collapse: Contradictions and neo-modernisation

    Directory of Open Access Journals (Sweden)

    Richard Sakwa

    2013-01-01

    Full Text Available Over two decades have passed since the dissolution of the communist system and the disintegration of the Soviet Union in 1991 yet there is still no consensus over the causes and consequences of these epochal (and distinct events. As for the causes, it is easy to assume that the fall was ‘over-determined’, with an endless array of factors. It behoves the scholar to try to establish a hierarchy of causality, which is itself a methodological exercise in heuristics. However, the arbitrary prioritisation of one factor over another is equally a hermeneutic trap that needs to be avoided. Following an examination of the various ‘why’ factors, we focus on ‘what’ exactly happened at the end of the Soviet period. We examine the issue through the prism of reformulated theories of modernisation. The Soviet system was a sui generis approach to modernisation, but the great paradox was that the system did not apply this ideology to itself. By attempting to stand outside the processes which it unleashed, both society and system entered a cycle of stagnation. The idea of neo-modernisation, above all the idea that societies are challenged to come to terms with the ‘civilisation of modernity’, each in their own way, provides a key to developments. In the end the Soviet approach to this challenge failed, and the reasons for this need to be examined, but the challenge overall remains for post-communist Russia.

  12. TERM OF THE PATENT. PREMISES FOR THE CREATION OF THE SUPPLEMENTARY PROTECTION CERTIFICATE

    Directory of Open Access Journals (Sweden)

    BUCURA IONESCU

    2013-05-01

    Full Text Available The legal nature of the rights derived from the patent was object of numerous theories and discussions in literature. Their main features represent recognized characteristics for the property right, nevertheless the limitation in time, in space and the ubiquity make the difference. Especially for new medicinal or plant protection products, due to the limitation in time, the period of effective protection under the patent is insufficient to cover the investment put into the research. There exists a risk of research centres situated in the Member States relocating to countries that offer greater protection. The uniform solution at Community level was created in form of regulations, as the most appropriate legal instrument to prevent the heterogeneous development of national patent laws affecting the free movement of products in the internal market. The duration of the protection granted by the patent may be extended to additional 5 years, by a supplementary protection certificate, granted, under same conditions provided by the regulation, by each Member State. The Community regulations created a legal form of a new national sui generis right, belonging both to the intellectual property right, namely patent right, and the administrative right of the marketing authorization. The main objective of the paper consists in informing the Romanian specialists in the field about the latest evolutions in intellectual property rights, especially in protection of the inventions, as a consequence of Romania’s accession to the European Community.

  13. Normativity of Scientific Law in the Perspective of Neo-Kantian Schools of Thought

    Directory of Open Access Journals (Sweden)

    FX. Adji Samekto

    2017-03-01

    Full Text Available Scientific normativity of law conceived as a character inherent in legal science as a sui generis. Jurisprudence basically studies the law, something that initially emerged from the dogmatic belief in philosophy. Dogmatism refuse to alter beliefs one iota. The teachings of dogmatic philosophy stem from the teachings of Plato and reflected in the legal enforceability. Dogmatism in the law is reflected in the Corpus Juris Civilis. Along with the development of post Era Scholastic philosophical thinking, the philosophy synthesizes thought between dogmatic thinking and skeptic has appeared in the Age of Enlightenment. This idea is reflected in Transcendental Idealist philosophy thought of Immanuel Kant. The core idea is that real human beings are given the ability to understand based on empirical experience and actually also able to gain an understanding of the human being that is the essence of symptoms. Transcendental Idealist, thus dynamic, moving to look for values that are useful for life. Transcendental Idealist thought then be adopted Kelsen in the teaching of normativity in legal positivism. Normativity in the teachings of Hans Kelsen’s legal positivism derived from the integration of empirical positivism and idealistic empiricism.

  14. Galerías mineras: la modelización analítica como una ayuda geomecánica para el diseño minero

    Directory of Open Access Journals (Sweden)

    Alvaro Correa Arroyave

    1991-09-01

    Full Text Available Después de una introducción en la que se esbozan los métodos analíticos utilizados en la Geotecnica Aplicada y se analizan las dificultades inherentes a cada método, se cuestiona por qué la Geomecánica no ha llegado a las minas tan rápido como se hubiera querido. En efecto estas obras, sui generis por su actividad, se encuentran en nuestro país más al abrigo de la experiencia adquirida in situ que a las aplicaciones de los desarrollos geomecánicos logrados en otros países. Posiblemente el desconocimiento o quizás la falta de credibilidad en estos logros, es la explicación de la ausencia casi total en la utilización de aquellos métodos en nuestro medio. Siendo la determinación de los datos de entrada el mayor problema práctico para llevar a buen término cualquiera de los métodos analíticos. el texto recomienda una metodología que permite cuantificar aquellos parámetros, y en especial las características geomecánicas de esfuerzo, deformación y resistencia de las masas rocosas.

  15. CRIMINAL LAW PROTECTION OF DATABASE AT A GLANCE

    Directory of Open Access Journals (Sweden)

    LUCIAN T. POENARU

    2012-05-01

    Full Text Available Database protection is provided in Romania by the general law on copyright no. 8/1996. According to the law, it is considered to be a crime making available to the public, by any means, the special rights attributed to database owners or copies thereof. This paper will focus on, one hand, presenting the way database and database related products can be subject to a copyright general protection and, on the other, revealing the special sui generis right attributed to database owners. In such a context, criminal instruments for protecting such rights seem to be quite annoying for the perpetrator, but less effective when it comes to a proper enforcement by the criminal bodies. This paper will therefore try to compare the way guilty actions of the culprit are effectively sanctioned by the criminal instruments provided by the law.And because the Romanian law on copyright does follow at least the letter of the European Directives on copyright and the protection of database, this paper will also search the spirit of the relevant European case-law and its applicability by the Romanian authorities.

  16. The Kosovo Precedent in the Secession and Recognition of Crimea

    Directory of Open Access Journals (Sweden)

    Dr.Sc. Elvina Jusufaj

    2015-06-01

    Full Text Available Crimea’s secession from Ukraine and its annexation to the Russian Federation invoked Kosovo precedent, in its declaration of independence, as an argument for secession. The territorial referendum in the Autonomous Republic of Crimea, only five days after the declaration of independence, was an attempt to justify the secession based on the right to selfdetermination of the people of Crimea. It is overwhelmingly considered illegal and its outcome has not been accepted and recognized by states, regional and international organizations. The comparative elements of statehood and secession between Kosovo and Crimea are reflected through analyzing the declarations of independence, international recognition and Russia’s role as a third-state factor in external selfdetermination. Essential distinctions are highlighted. Kosovo is widely acknowledged and accepted a sui generis case. Its declaration of independence came as result of a long monitored comprehensive process; not to legitimize the right for self-determination but as the final option for stability and peace in the region. Crimea seceded in violation of international law through the use of force. While Kosovo is a democratic, multi-ethnic new state and recognized by 107 states, the secession of Crimea and its annexation to the Russian Federation is considered illegal and endangers the existing international order.

  17. Principles of land debt as a kind of real collateral

    Directory of Open Access Journals (Sweden)

    Pavićević Aleksandra

    2014-01-01

    Full Text Available The issue of the work is an analysis of characteristics of land debt, known as non-accessory real right securing claims in German and Swiss law. Using the method of comparative analysis of the characteristics of this property right, author determines the similarities and differences of land debt in relation to similar types of rights, in particular in relation to a mortgage, as most similar security right, from the group of liens. Since the Draft Code of ownership and other property real rights in Serbia in 2006. proposed the introduction of real debt in Serbian law, the author of the paper examines the advantages and disadvantages of this concept in order to evaluate this assessment.. As an essential difference to the lien, the author defines the non-accessory character of land debt and multifunctional purpose. The author advocates the introduction of institute of land debt in future Serbian law, as the original non-accessory real security right (sui generis that represents functional addittion to accessory mortgage.

  18. LA CONSAGRACIÓN JURÍDICA DE TESTIGOS OLVIDADOS. EL JUEZ ARGENTINO FRENTE AL GENOCIDIO ARMENIO

    Directory of Open Access Journals (Sweden)

    Sévane Garibian

    2015-09-01

    Full Text Available “El Estado turco ha cometido el delito de genocidio contra el Pueblo Armenio, en el periodo comprendido entre los años 1915 y 1923”. Esta declaración, realizada por un juez federal argentino el primero de abril de 2011 en Buenos Aires, se encuentra en el núcleo de una sentencia definitiva que constituye una primicia mundial y conlleva la consagración jurídica –el (reconocimiento– de los testigos olvidados de un crimen negado por Turquía. La sentencia se inscribe en un marco singular y único en su género: la práctica judicial sui generis de los juicios por la verdad, auténtica especificidad nacional creada en Argentina en los años noventa para revertir la política del olvido de la época en lo relativo a los crímenes de la dictadura militar. Una práctica cuya existencia misma es el producto directo de una situación inicial de borrado e impunidad, que incita a la creación de mecanismos judiciales alternativos de certificación del hecho criminal y de consagración de sus víctimas/testigos.

  19. Breaking Bad: Intertextuality, Intermediality and Crossmediality, and the Taste of Unpredictability

    Directory of Open Access Journals (Sweden)

    Nicola M. Dusi

    2016-07-01

    Full Text Available Breaking Bad è una serie per molti aspetti indecidibile nel genere, che presenta un’intertestualità aperta e cangiante, tra serialità televisiva e cinema d’autore (ad esempio tra western e poliziesco, e si apre all’innovazione intermediale, data non solo dall’ibridazione dei generi e della fiction seriale con il cinema, ma dai modi metadiscorsivi (o 'metacinematografici' e da soluzioni che lavorano sui bordi dei media e sulla loro porosità grazie alle nuove tecnologie digitali. Passando attraverso esempi di modi traduttivi e reinterpretativi, intertestuali e intermediali, transmediali e crossmediali, cercherò di analizzare alcuni meccanismi di funzionamento degli attuali ecosistemi mediali seriali in Breaking Bad. Ad esempio nelle citazioni dirette e indirette, nelle aperture intertestuali e intermediali, ma anche nei teaser e nei recaps, nei webisode e nelle proliferazioni crossmediali delle pratiche dei remixer, distinguendo tra ripetizione e innovazione, tra modi centripeti e centrifughi dello storytelling e, per i remix, tra modi della selezione e modi della collezione.

  20. Quando o princípio do absurdo disparou a idéia psicanalítica: uma concepção da Teoria dos Campos When the principle of absurd triggered the pschycoanalitic idea: a conception of the Theory of Fields

    Directory of Open Access Journals (Sweden)

    Andrea Giovannetti

    1999-01-01

    Full Text Available O artigo trata da noção das origens da Psicanálise não como uma invenção sui generis de Freud, mas antes como uma apropriação da idéia interpretativa, cuja essência, a interpretação, ele transformou em método. Sob este ângulo evidencia-se claramente que a disciplina psicanalítica não se restringe a uma prática terapêutica, ainda que o momento histórico que engendrou seu aparecimento a tenha convocado a tratar da crise, o absurdo que se instalara no âmago da sociedade urbana do início do século, a princípio na forma das neuroses.The article considers the notion of the origins of Psychoanalysis not as something that Freud actually invented, but as an appropriation of the interpretative idea, whose essence, the interpretation, he turned into a method. From this standpoint, it becomes obvious that Psychoanalysis as a discipline is not limited to a psychotherapeutic technique. The historic moment that required its appearance summoned it to heal the crisis, the absurdity, that had set up in the bosom of the urban society of the beginning of the century, crisis that at first presented itself in the shape of the neuroses.

  1. Below the radar innovations and emerging property right approaches in Tibetan medicine.

    Science.gov (United States)

    Madhavan, Harilal

    2017-11-01

    Outside the established legal framework of intellectual property rights, countries have pursued multiple pathways to protect and promote traditional medicine. As Tibetan medicine is a late entrant into commercialization, the proposals to propertize generally fall within the rationale of existing sui-generis paradigms of Intellectual property. In this context, the article enquires the state of innovations in this sector viz-a-viz the property right approaches in place especially in India and China. It argues that beyond the usual complex medical science and technology led-innovations, the pathways of cumulative processes and creative additions through informal experiential learning platforms, where the transfers of knowledge become part of livelihood and social benefits (we call them "below the radar innovations") is ubiquitous in Tibetan medicine. The trends and politics in two recent strategies of protection, that is, Tibetan medicine as economic property (emphasizing patents here among many others) and as a cultural property (intangible cultural heritage) are juxtaposed with these informal innovative attempts. The paper underlines that the productivity-based economic rationale of these protection mechanisms should not obscure sustainability alternatives of "below the radar" (BtR) innovations in Tibetan medicine.

  2. La fuerza vinculante de la jurisprudencia en el orden jurídico colombiano

    Directory of Open Access Journals (Sweden)

    Carlos Bernal Pulido

    2003-12-01

    Full Text Available El carácter de la fuente formal y material del derecho que hoy tiene la jurisprudencia no es una innovación de la Constitución Política de 1991. El artículo propone una definición operativa de fuerza vinculante, muestra cómo los elementos de esa definición se verifican en la jurisprudencia y cuál es el sentido constitucionalmente adecuado que se ha de otorgar entonces a la expresión “criterio auxiliar” en el artículo 230 de la Constitución. La argumentación hace ver por qué el trabajo de aplicación judicial del derecho lleva consigo la creación judicial de normas jurídicas contenidas en la parte motiva de las sentencias. Tales normas, que deberían llamarse adscritas, resultan vinculantes para las altas cortes de manera sui generis. Es necesario diseñar un procedimiento de control de la sujeción de los jueces ordinarios a la jurisprudencia constitucional, que también formule de manera explícita las reglas a las que haya de atenerse la Corte Constitucional.

  3. Structure–function relations in the NTPase domain of the antiviral tRNA ribotoxin Escherichia coli PrrC

    International Nuclear Information System (INIS)

    Meineke, Birthe; Shuman, Stewart

    2012-01-01

    Breakage of tRNA by Escherichia coli anticodon nuclease PrrC (EcoPrrC) underlies a host antiviral response to phage T4 infection. Expression of EcoPrrC is cytocidal in yeast, signifying that PrrC ribotoxicity crosses phylogenetic domain boundaries. EcoPrrC consists of an N-terminal NTPase module that resembles ABC transporters and a C-terminal nuclease module that is sui generis. PrrC homologs are prevalent in many other bacteria. Here we report that Haemophilus influenzae PrrC is toxic in E. coli and yeast. To illuminate structure–activity relations, we conducted a new round of mutational analysis of EcoPrrC guided by primary structure conservation among toxic PrrC homologs. We indentify 17 candidate active site residues in the NTPase module that are essential for toxicity in yeast when EcoPrrC is expressed at high gene dosage. Their functions could be educed by integrating mutational data with the atomic structure of the transition-state complex of a homologous ABC protein.

  4. Guidelines for a Comprehensive Care Program to Ostomized Patients and Families: a Nursing proposal

    Directory of Open Access Journals (Sweden)

    Paula Alvarenga de Figueiredo

    2016-01-01

    Full Text Available Objectives: describe care needs and demands that mark the discursive practices of ostomized clients and family members and discuss guidelines for a comprehensive care program to ostomized clients and their families, organized by macrosociological categories. Method: Creative and Sensitive, involving 17 ostomized subjects and family members at a municipal outpatient clinic. The ethical aspects were complied with. A characterization form was used, as well as Creativity and Sensitivity Dynamics: "speaking map", "body-knowledge" and "calendar". Critical Discourse Analysis was applied. Results: the health needs and care demands of the ostomized patients and their family members, in their multiple dimensions, were constituted in the home and community, outpatient and social context, implying new orientations for nursing care. The unveiling of the data brought elements that constituted guidelines, in a macrosociological approach, to achieve the expanded integrality of nursing care. Conclusion: the ostomized clients are unique in their genre/peculiar from Latin sui generis, calling for strategies that respond to and distinguish their specificities. Elaborating a Public Health Policy that improves and reorganizes the care demands, taking into account these individual biopsychosocial and spiritual aspects, is a possible and irrevocable target in the attempt to achieve better conditions of health and wellbeing.

  5. Reificação e consumismo ostentatório no Gatsby O Magnifíco Reification and consumerism in The Great Gatsby

    Directory of Open Access Journals (Sweden)

    Robert Sayre

    2007-05-01

    Full Text Available Trata-se de uma interpretação do romance Gatsby, o Magnífico, de F. Scott Fitsgerald, a partir de aspectos essenciais da sociedade moderna, como a reificação e o consumo ostentatório, aspectos esses intimamente ligados e relativamente distintos. Trata-se, portanto de destacar algumas modalidades importantes de conhecimento sui generis desses fenômenos, exclusivamente, pelos meios literários. Palavras-chave: Alienação. Classe ociosa. Reificação. Literatura. This paper is a sociological interpretation of the novel The Great Gatsby, by F. Scott Fitsgerald, from the point of view of some of the essential aspects of modern society such as the reification and the boasting consume which are at the same time closely linked and relatively distinct. In fact, it tries to point out some important modalities of human knowledge through a literary form, the novel. Keywords: Alienation. Idle class. Literature. Reification.

  6. `elyon as hypostasis of Ahura Mazda – a reading of Dt 32.8-9

    Directory of Open Access Journals (Sweden)

    Osvaldo Luiz Ribeiro

    2016-12-01

    Full Text Available The paper postulates the hypothesis of reading of Dt 32.8-9 as monolatrous and post-exilic narrative, written in context of political and cultural subordination to the Persian hegemony, whose Empire depends relentlessly on the restoration and preservation of Judah. Dt 32,1-43 would answer for the sui generis characterization of the theology of the text that, despite the monolatrous theology embodied in the passage and the expressed horror at the idea of the nation of Yahweh to worship other gods. The textual composition is compelled to assume the thesis that Yahweh's exclusive ownership of Judahis due to the determination of `elyôn, which is then interpreted, as the hypostasis of Ahura Mazda and that, in geopolitical terms, embodies the very Persian Empire. It is assumed therefore that Dt 32.8-9 is a political-theological passage of legitimation of Judahite political-theological system in the light of the Persian geopolitical system.

  7. Financial contagion reloaded: the case of Cyprus

    Directory of Open Access Journals (Sweden)

    Iulia Monica Oehler-Sincai

    2013-05-01

    Full Text Available In the present case study, our main objective is to bring to the forefront the main factors that led to the double-dip recession of the Cypriot economy. We analyze determinants such as “tax haven” status, interlinks with the Greek economy, spillovers originating in the Euro Area as a whole (through the debt crisis and Greece in particular. In spite of the fiscal reform of 2002 and the new fiscal system in force since the 1st of January 2003, Cyprus continued to be a “tax haven”. Its high investment attractiveness spurred the “Cyp-Rus” relations, boosted an outsized banking sector (representing 800% of Cyprus GDP in 2010-2011 and generated an unsustainable proportion between the real and the “virtual” economy. Besides, the public sector became larger than required by such a small economy, its share in the total yearly value added being close to one quarter. Taking into consideration all these factors, we conclude that the „W”-shaped recession of the Cypriot economy is a sui generis one and the questioning of its “fiscal paradise” status is even more acute than in the case of countries such as Luxembourg or Liechtenstein.

  8. Henri Bergson y George Bernard Shaw: hábito, vida, muerte

    Directory of Open Access Journals (Sweden)

    Álvaro Cortina Urdampilleta

    2015-01-01

    Full Text Available Este artículo indaga en algunos aspectos de la antropología filosófica expuesta en la obra del francés Henri Bergson. Para ello, nos serviremos de la confrontación de su doctrina original con la de un bergsoniano sui generis, el intelectual y dramaturgo irlandés George Bernard Shaw. El Prefacio. A medio siglo infiel, de Shaw, incluido en su obra más ambiciosa, Vuelta a Matusalén. Un Pentateuco Metabiológico, de 1921, será el texto base con el que haremos dialogar a Bergson con este periférico bergsonismo. Tomaremos como hilo conductor la teoría sobre el hábito, en el contexto de la metafísica de la evolución de los seres orgánicos, y lo llevaremos hasta el concepto límite, que parece conciliar ambas posturas: la idea de muerte superable. Ni en Bergson ni en Shaw los hábitos (la Cultura forman parte integral de la esencia del hombre, y la muerte (cuestión en la que Bergson es mucho más elusivo juega en ellos un rol anfibio, entre Cultura y Naturaleza.

  9. Spécificité pastorale de la personnalité de Jean-Paul II selon Jean-Marie Lustiger

    Directory of Open Access Journals (Sweden)

    Ryszard Biernat

    2011-04-01

    Full Text Available Jean-Marie Lustiger admired John Paul II and he was devoted to him. They both had similar origin. The Cardinal of Paris often accompanied the pope in his pastoral visits. He perceived John Paul II as the man of Providence. He was convinced that God had prepared him to this role. Jean-Marie Lustiger emphasized the attitudes of John Paul II that attracted the attention and admiration of observers. For instance: pray, holiness, attentive pastor and good relation with young people. Jean-Marie Lustiger as an attentive observer also focused his attention on the pastoral methods of John Paul II. In his utterances he often perceived the following methods: each person was important, defense of person’s dignity, the ability to speak with each man, modern language, importance of the pastoral visits and sui generis spiritual pedagogic. Jean-Marie Lustiger noticed radical change of perspective in preaching and in pastoral action of John Paul II. According to him the pope perceived the challenges of the modern times, evaluated needs of the humanity and gave the appropriate answers. For these he did not choose politics, sociology or even anthropology as a re fl ection but addressed to the hurt human who was redeem by Jesus Christ.

  10. LONGITUDINAL EVALUATION OF ACADEMIC DEPARTMENTS USING DATA ENVELOPMENT ANALYSIS

    Directory of Open Access Journals (Sweden)

    Rodrigo Meleu das Neves

    2016-11-01

    Full Text Available Corresponding to the smallest structure in a University for educational-academic, administrative and personnel allocation purposes, the academic department is currently a sui generis entity. The research proposes a temporal analysis of the relative efficiency of 93 academic departments at Federal University of Rio Grande do Sul (UFRGS over the period of 1998 through 2007, using the mathematical modeling technique called data envelopment analysis, as well as indicators equally related to education, research and extension. Among other conclusions, it can be observed that adding a professor to the faculty does not proportionally impact productivity; actually, the synergy may bring even better results. Likewise, removing a professor may not proportionally decrease department output. Furthermore, the research traces a relationship between the results obtained in the replicated model and the model practiced to establish a criteria of faculty position distribution: the department index. The authors present their findings by means of a mixed method technique. The homogeneity of those departments more dedicated to research and the increasing investment on the part of UFRGS in extension evidence the contrast between models, even considering that reorganizations in the system employed for this analysis may leave room for questioning.

  11. Traditional Knowledge and Benefit Sharing After the Nagoya Protocol: Three Cases from South Africa

    Directory of Open Access Journals (Sweden)

    Roger Chennells

    2013-09-01

    Full Text Available The Nagoya Protocol of the Convention on Biological Diversity (CBD has finally produced a negotiated framework intended to significantly advance the achievement of its core objectives, chief amongst them benefit sharing with indigenous and local communities who are holders of traditional knowledge related to genetic resources. The interpretation, in particular of central concepts contained in the Protocol, namely traditional knowledge (TK, community, and ownership of TK, and the practical application thereof by governments, are key to the success of the emerging access and benefit sharing regime. This article examines the manner in which the South African Biodiversity Act deals with these concepts. Three recent case studies are described, namely the Hoodia, Sceletium and Pelargonium cases, in which a range of issues relating to holders of TK were resolved, including the question of who the indigenous knowledge holders are. Moreover the debate on the question as to whether the intellectual property rights of TK holders are property rights as such, leads to the author’s suggestion that TK rights are a sui generis form of property rights, and that the legal principles contained in the law of equity provide useful and accessible guidance towards resolution of potentially competing claims of TK rights by indigenous peoples.

  12. UMA ARQUITETURA DA INFORMAÇÃO PARA PORTAIS DA INOVAÇÃO DAS UNIVERSIDADES PÚBLICAS BRASILEIRAS

    Directory of Open Access Journals (Sweden)

    Olival de Gusmão Freitas Júnior

    2013-10-01

    Full Text Available A inovação se apresenta na sociedade do conhecimento como um fator preponderante no processo de desenvolvimento das nações. De forma sui-generis, enquanto em quase todo mundo a inovação se faz na empresa, no Brasil a Universidade Pública Brasileira é um atípico e presente ambiente de inovação. A interação universidade-empresa define um processo de cooperação destinado a gerar produtos, serviços e processos, devendo ser intensificado e orientado com mais eficácia para a inovação e, de forma genérica, para a transferência de conhecimentos. Neste contexto, observa-se a necessidade nas Universidades Públicas Brasileiras de criar um ambiente virtual de inovação com os seus diversos atores, promovendo compartilhamento e disseminação do capital intelectual na busca de soluções inovadoras e construção de estratégias para o desenvolvimento social. Este artigo objetiva apresentar uma arquitetura informacional para o portal da inovação aplicado às Universidades Públicas Brasileiras que se constituirá em um espaço de cooperação entre setor produtivo e comunidade científico-tecnológica.

  13. The EU’s Post-Lisbon Democratic Development: What Lessons for Iceland?

    Directory of Open Access Journals (Sweden)

    Maximilian Conrad

    2014-06-01

    Full Text Available Apart from the question of whether permanent exemptions from EU rules could be achieved in Iceland’s (by now halted EU accession negotiations, the EU’s institutional development in the wake of the Lisbon Treaty has been used as a key argument for the conservative government to first suspend the negotiations and subsequently also to propose to withdraw the membership application altogether. In this regard, concerns about the democratic quality of EU decision making play a key role. However, as this article argues, the institutional development of the EU since Lisbon, particularly in relation to democratic governance, has been considerably more subtle than it is claimed to be and has to be seen as a continuation of a much longer process. More importantly, however, the debate leaves pressing questions about the nature of the EU as a polity unaddressed. In this context, this article addresses the question of what kind of democracy is possible in the kind of polity sui generis that the EU undoubtedly is.

  14. THE POSITION OF JUVENILES IN THE NEW CRIMINAL LAW OF THE REPUBLIC OF SERBIA

    Directory of Open Access Journals (Sweden)

    Dragan Jovašević

    2008-01-01

    Full Text Available The new juvenile (substantive, procedural and executive criminal law came into force at the beginning of 2006 in the Republic of Serbia.. In this way, by concluding its reform of criminal law, the Republic of Serbia followed the trends of modern criminal policies of other developed European countries (France, Germany, and Croatia. Therefore, in that special, specifi c way, it determined the criminal legal status of juveniles. That specifi city is refl ected in various directions : 1 Lex specialis was brought in – a special Act on juvenile perpetrators of criminal acts and the criminal legal protection of juveniles when juveniles in their criminal legal position are completely separate from the status of adults as perpetrators of criminal acts, 2 the special authority of district courts is determined for taking action in criminal cases of juvenile perpetrators of criminal acts, 3 compulsory specialisation is provided for persons in the criminal judiciary taking part in criminal proceedings for juvenile perpetrators of criminal acts ( with previous training and issuing of licences ‘certifi cates’ and 4 besides criminal sanctions, the law has provided for juvenile perpetrators of criminal acts the possibility of sentencing specifi c measures sui generis – educational orders ( directions or recommendations – as means of restorative justice by which the commencement or carrying out of legal action is avoided. This paper precisely deals with this new criminal legal position of juvenile perpetrators of criminal acts and with the new institutions of restorative justice from theoretical, practical and comparative legal aspects.

  15. Electroweak symmetry breaking: Higgs/whatever

    International Nuclear Information System (INIS)

    Chanowitz, M.S.

    1990-01-01

    In these two lectures the author discusses electroweak symmetry breaking from a general perspective, stressing properties that are model independent and follow just from the assumption that the electroweak interactions are described by a spontaneously broken gauge theory. This means he assumes the Higgs mechanism though not necessarily the existence of Higgs bosons. The first lecture presents the general framework of a spontaneously broken gauge theory: (1) the Higgs mechanism sui generis, with or without Higgs boson(s) and (2) the implications of symmetry and unitarity for the mass scale and interaction strength of the new physics that the Higgs mechanism requires. In addition he reviews a softer theoretical argument based on the naturalness problem which leads to a prejudice against Higgs bosons unless they are supersymmetric. This is a prejudice, not a theorem, and it could be overturned in the future by a clever new idea. In the second lecture he illustrates the general framework by reviewing some specific models: (1) the Weinberg-Salam model of the Higgs sector; (2) the minimal supersymmetric extension of the Weinberg-Salam model; and (3) technicolor as an example of the Higgs mechanism without Higgs bosons. He concludes the second lecture with a discussion of strong WW scattering that must occur if L SB lives above 1 TeV. In particular he describes some of the experimental signals and backgrounds at the SSC. 57 refs., 12 figs

  16. Explanatory models for homeopathy: from the vital force to the current paradigm.

    Science.gov (United States)

    Waisse, Silvia; Bonamin, Leoni Villano

    2016-08-01

    Facing claims for and against the scientific status of homeopathy, one is entitled to ask: is there a scientific model for homeopathy? In this study we reconstructed the model put forward by Hahnemann. The results showed that it was essentially based on the assumption of a 'vital force' exclusive to living beings. While the vital force was a basic element of 18th-century science, the existence of such a sui generis force of nature was refuted with the formulation of the law of the conservation of energy by mid-19th century. As a function of that fact for homeopathic theory, we discuss the history of the rise and demise of the theory of the vital force from the last quarter of the 18th century to 1830. Finally, we call the attention to the paradigm shift biology underwent starting at the end of the 19th century as the framework for contemporary views on the functioning of living beings and consequently, of the effects of pharmacological agents on them. Copyright © 2016 The Faculty of Homeopathy. Published by Elsevier Ltd. All rights reserved.

  17. Philology as curatorship: The Pessoa affair

    Directory of Open Access Journals (Sweden)

    Pedro Tiago Ferreira

    2016-12-01

    Full Text Available The argument presented throughout this essay is that philology is textual curatorship. “To curate” means “to administer someone else’s assets”; to edit a text is an activity according to which the textual critic administers someone else’s text, which is that author’s asset; therefore, the textual critic is a textual curator. There are two difficulties which this assertion raises, which can be formulated as follows: how can an incorporeal asset be administered? How can one administer texts whose characteristics are similar to those which make up Fernando Pessoa’s work? These texts are incomplete because they were left unfinished, not because portions of them have been lost. When this occurs, it is necessary to understand that besides being a textual curator, the textual critic becomes a sui generis co-author as well, as the critic assumes the responsibility of making decisions which are typically made by authors of literary works.

  18. The Balkan balneotherapy product – an approach from the destination marketing perspective

    Directory of Open Access Journals (Sweden)

    Aurelia-Felicia STĂNCIOIU

    2013-10-01

    Full Text Available Considering the common features in the resources field, but especially the way in which they have been managed at the level of each individual country, it can be stated that a destination such as the Balkan peninsula represents a culturally homogeneous space, which has been crystallized over centuries of common history and which has imprinted a “common” destiny to the member countries, regarding the economic and cultural development. Regarding natural resources, the main similarity resides in the existence of balneotherapy resources, with particular therapeutic properties, nowadays’ development being pressured by the deterioration of one’s conception about its own health (from the demand perspective, on the one hand, and extending the concept of health (from the supply perspective, on the other hand. In the context of a less-controlled development of certain methods/techniques/procedures by the stakeholders of wellness industry, it is necessary to maintain the authenticity of the balneotherapy product whose essence is the treatment. The purpose of the paper relies in establishing the common elements which could form the essence of the balneotherapy product (sui generis and, in the same time, the differentiation elements which form the developed product of each Balkan country, alongside with the establishment of joint actions, in order to set the coordinates of the regional balneotherapy marketing strategy for the Balkan peninsula.

  19. Structure-function relations in the NTPase domain of the antiviral tRNA ribotoxin Escherichia coli PrrC

    Energy Technology Data Exchange (ETDEWEB)

    Meineke, Birthe; Shuman, Stewart, E-mail: s-shuman@ski.mskcc.org

    2012-06-05

    Breakage of tRNA by Escherichia coli anticodon nuclease PrrC (EcoPrrC) underlies a host antiviral response to phage T4 infection. Expression of EcoPrrC is cytocidal in yeast, signifying that PrrC ribotoxicity crosses phylogenetic domain boundaries. EcoPrrC consists of an N-terminal NTPase module that resembles ABC transporters and a C-terminal nuclease module that is sui generis. PrrC homologs are prevalent in many other bacteria. Here we report that Haemophilus influenzae PrrC is toxic in E. coli and yeast. To illuminate structure-activity relations, we conducted a new round of mutational analysis of EcoPrrC guided by primary structure conservation among toxic PrrC homologs. We indentify 17 candidate active site residues in the NTPase module that are essential for toxicity in yeast when EcoPrrC is expressed at high gene dosage. Their functions could be educed by integrating mutational data with the atomic structure of the transition-state complex of a homologous ABC protein.

  20. Un mundo rural en transición. La campaña bonaerense según el Primer Censo Nacional de Población de la República Argentina (1869

    Directory of Open Access Journals (Sweden)

    Claudia Contente

    2016-01-01

    Full Text Available La campaña de Buenos Aires había recibido desde fines del siglo XVIII importantes corrientes migratorias interprovinciales que fueron progresi - vamente desplazadas por las provenientes de Europa. Si bien existen tra - bajos basados en los resultados generales, se ha abordado muy poco el estudio de la población a partir de las cédulas censales en sí mismas. Nos proponemos aquí observar la estructura de la población de la campaña bonaerense durante estos primeros tiempos de la inmigración masiva, aplicando una perspectiva comparada entre tres pueblos: San Antonio de Areco en la zona norte, Mercedes en el centro y San Vicente en el sur. Se prestará particular atención a la composición de la población, su origen, las actividades productivas y el proceso de urbanización. Se analizan igualmente las pautas de destino y localización geográfica de los extranjeros en los pueblos rurales, las ocupaciones preferidas y la eventual diversificación de actividades en función de las nacionalidades.

  1. Census of land use and water as tools of information and collaboration with social partners; Censos del uso de la tierra y el agua como herramientas de informacion y colaboracion con los agentes sociales

    Energy Technology Data Exchange (ETDEWEB)

    Blazquez Perez, D.; Ortiz Trujillo, D.; Perez Fonseca, A.; Sierra Gil, S.

    2011-07-01

    This paper tries to present the development of the Census Land Use and Water as an instrument of the operator of the facility to enhance the knowledge of the environment of nuclear facilities, as well as a tool for communication and collaboration with the municipalities, in order to a closer and transparency with the social partners in decision making affecting such facilities.

  2. Identidad ética y redes sociales: El debate identitario en las comunidades afrodescendientes de Orkut enel contexto del censo demográfico brasileño de 2010

    OpenAIRE

    Rodrigues Santos, Elza Fátima

    2016-01-01

    Las relaciones raciales en Brasil y las redes sociales constituyen un ámbito de estudio poco explorado en tanto en las universidades brasileñas como en las españolas. Nos resulta relevante desarrollar líneas de investigación que permitan una aproximación científica a estos fenómenos sociales, donde se manifiestan la representación, la mediación comunicativa, la identidad, la negritud y las nuevas tecnologías. Límites de la investigación La investigación tiene como marco temporal los años 2009...

  3. Reflexiones metodólogicas a partir de los datos e instrumentos de los censos de población y vivienda de 2000 y 2010 para el Estado de México

    Directory of Open Access Journals (Sweden)

    Juan Gabino González Becerril

    2012-01-01

    lugar de nacimiento y su lugar de residencia cinco años antes del levantamiento censal. Haciendo uso de las matrices y tasas podremos ubicar a entidades receptoras y expulsoras en torno a la movilidad de su población, así como identificar las características individuales de la población migrante. La muestra censal permite contar con información actualizada sobre los intercambios cotidianos de población laboral entre los municipios y entidades del país.

  4. Electric utilities strategies in final energy markets; Nuove strategie d'impresa sui mercati finali dell'energia

    Energy Technology Data Exchange (ETDEWEB)

    Bianchi, A. [RIE S.r.L., Bologna (Italy)

    2000-07-01

    In rapidly changing markets, electric utilities pay growing attention to customers and service. They are aware that competition needs strategies capable of transforming and strengthening the privileged position resulting from the knowledge of the market. Moreover, this aspect is the link between different value chains to describe new multi utility approaches. [Italian] In mercati in rapida evoluzione, cresce nelle compagnie energetiche l'attenzione al cliente e al servizio; la concorrenza va affrontata con strategie di trasformazione e rafforzamento della posizione di privilegio che la conoscenza del mercato offre. Questo elemento rappresenta poi il ponte tra catene del valore diverse, verso un nuovo approccio multi-servizi.

  5. L'Accordo dell'Uruguay Round: una prima valutazione dei suoi effetti sull'UE e sui PSV

    Directory of Open Access Journals (Sweden)

    C. MASTROPASQUA

    1994-09-01

    Full Text Available On December 15th of last year, seven years after its start, the Uruguay Round negotiations for the reform of the General Agreement on Tariffs and Trade came to a conclusion. This paper aims to provide a qualitative assessment of the results achieved in the fields of agriculture, market access, trade rules and intellectual property protection, leaving out services, given the limited results of liberalisation achieved in this area. In this limited context, the focus is on the European Union and developing countries. The authors describe the details of the agreement by sector before analysing the effects on the EU and developing countries. The effects of intellectual property protection both industrial countries and developing countries is then assessed.

  6. L'Accordo dell'Uruguay Round: una prima valutazione dei suoi effetti sull'UE e sui PSV

    Directory of Open Access Journals (Sweden)

    C. MASTROPASQUA

    2013-10-01

    Full Text Available On December 15th of last year, seven years after its start, the Uruguay Round negotiations for the reform of the General Agreement on Tariffs and Trade came to a conclusion. This paper aims to provide a qualitative assessment of the results achieved in the fields of agriculture, market access, trade rules and intellectual property protection, leaving out services, given the limited results of liberalisation achieved in this area. In this limited context, the focus is on the European Union and developing countries. The authors describe the details of the agreement by sector before analysing the effects on the EU and developing countries. The effects of intellectual property protection both industrial countries and developing countries is then assessed.  JEL Codes: F13 

  7. Gli interventi nazionali sui problemi della migrazione: strategie e programmi (National interventions on the issues of migration: strategies and programs

    Directory of Open Access Journals (Sweden)

    Carmine CLEMENTE

    2016-03-01

    Full Text Available Europe has become the first continent of attraction for migrants and is also the first continent as for incidence of the population emigrated to the total of population. In Italy, in particular, the foreign population has grown from 2000 to 2013 at an average rate of 10% per annum. This scenario, plus the emergence of immigrant landings on the Italian coast as a point of arrival but also as a point of departure to other European countries, leads to awareness of the need for answers, not only at the Italian institutional level but also within a European convergence framework, in the search of policies and programs most appropriate to a new social context, strongly characterized by the presence of the immigrant population

  8. Influenza di materie prime non convenzionali, grano saraceno e crusca, sui parametri qualitativi degli spaghetti in cottura

    OpenAIRE

    Protopapa, Andrea

    2006-01-01

    Al fine di valutare l’impiego di materie prime non convenzionali nella produzione industriale di pasta secca, 9 campioni di spaghetti sono stati prodotti impiegando, oltre al grano duro, percentuali crescenti di grano saraceno e crusca. Gli spaghetti ottenuti sono stati confrontati con uno standard commerciale di riferimento realizzato totalmente con semola. L’analisi sensoriale ha mostrato che all’aumentare del contenuto di grano saraceno e crusca si riducono il grado di ammassamento e di ad...

  9. Serological characterization and antimicrobial susceptibility of Streptococcus suis isolates from diagnostic samples in Denmark during 1995 and 1996

    DEFF Research Database (Denmark)

    Aarestrup, Frank Møller; Jorsal, Sven Erik Lind; Jensen, N. E.

    1998-01-01

    , ampicillin, ceftiofur, enrofloxacin, penicillin, spectinomycin, tiamulin and trimethoprim + sulphadiazine. A high frequency (> 30%) of resistance to tetracydine was observed. Among isolates of serotype 2, 9.7% were resistant to lincomycin and 12.9% to spiramycin. Among other serotypes 56.5% were resistant...

  10. Ancora sui tempi di tragitto delle Pn provenienti dalla Sicilia Tabelle numeriche per varie profondità ipocentrali

    Directory of Open Access Journals (Sweden)

    G. PANNOCCHIA

    1974-06-01

    Full Text Available This paper follows the foregoing studies by the A.A. already carried out on the earthquakes occurred in Western Sicily. The hypocentral data investigation concerning a further large earthquake of the swarm, has confirmed an hypocentral depth close to the "Molio". Grouping conveniently the investigated earthquakes it was possible to calculate the longitudinal waves travel-times curves at the following depths: 30, 35, 40, 45, 50, 00 km (A < 20°. Some interesting results concerning the first impulses of P„ waves have been found (existence of two series of waves, P,n and IJ„2 having a slightly different velocity. Five curves for each examined h have been calculated; the P„ (the general using all the onsets including the ones whose residuals resulted later to be about three seconds: the Pni and the P„2 representing a Pn subdivision obtained by reducing the residuals range: the P„ -^13° and the P„ (10°M 20° for 2» < A < 13° and 10° < A < 20° respectively calculated. (The P„ 13° approaches to a straight line, in accordance with other estimations for curves investigated along shorter paths. The paper contains also all numerical tables related to 29 travel-times curves calculated every 20 km; as well as the curve corresponding to li = 17.5 km. The last curve has been obtained averaging the values of depth from 5 km to 30 km in order to fill the gap 0 i—i 30 km.

  11. Alcune osservazioni sui fenomeni dell'ansia nell'apprendimento di una lingua straniera / Vittoriano Renó

    Index Scriptorium Estoniae

    Renó, Vittoriano

    2009-01-01

    Võõrkeeleõpingutega seotud ängist. Artiklis püstitatakse hüpotees, et õppejõu informaalne suhtumine võib aidata õpilastel teadmisi omandada, sest kõrvaldaks ängitekitavad faktorid, mis on seotud õppejõu ja õpilase vahelise sotsiaalse distantsiga

  12. L'utilizzo delle rivalutazioni da leggi speciali e l'impatto sui bilanci delle imprese italiane

    OpenAIRE

    Piras, Fabrizio

    2015-01-01

    In an institutional framework traditionally based on the historical cost accounting system, the revaluation permitted by special laws allow the recognition of fixed assets at fair value in order to mitigate the distorting effects of inflation. These laws are expected to help companies improve the depiction of a true and fair view of their financial position. This accounting practice generates an increase of both total assets and shareholders’ equity, thus strengthening the percept...

  13. Influenza del trattamento della schizofrenia con neurolettici tipici o olanzapina sui costi sanitari e sugli outcomes lavorativi

    Directory of Open Access Journals (Sweden)

    Angelomarco Barioglio

    2005-06-01

    Full Text Available The aim of the present study was to evaluate the global treatment cost of schizophrenia with olanzapine or typical neuroleptics, according to Local Health Care Unit point of view. This analysis was performed through naturalistic observation of a cohort of schizophrenic patients referring to Ascoli Piceno ASL Department of Mental Health during 4 years (2001-2004. During year 2003, investigators have identified a cohort of patients who were undergoing treatment switch from typical neuroleptics to olanzapine. These patients, after the switch, are observed prospectively for the next 2 years and retrospectively for the last 2 years of treament. This method allow us to collect data about 4 years of treatment: 2 years of typical neuroleptic treatment followed by 2 years of olanzapine treatment. The present work is presenting the analysis of the first 3 years of observation. The results of our analysis are demonstrating that olanzapine treatment, through a better patient-physician alliance and with rehabilitative activities, allow lower total medical costs for the treatment of schizophrenia than typical neuroleptics. The higher acquisition cost of olanzapine versus typical neuroleptics was compensated by less hospitalizations and territorial medical interventions. During olanzapine treatment patients followed more rehabilitative activities (+71,26%, p <0,0001 and increased their working activities (+39,13%, p< 0,001.

  14. Immune Responses and Protection against Experimental Brucella suis biovar 1 Challenge in Non-vaccinated or RB51-Vaccinated Cattle

    Science.gov (United States)

    Twenty Hereford heifers, approximately 9 months of age, were vaccinated with saline (control) or 2 x 10**10 CFU of Brucella abortus strain RB51 (RB51) vaccine. Immunologic responses after inoculation demonstrated significantly greater (P<0.05) antibody and proliferative responses to RB51 antigens i...

  15. Analisi del rapporto tra valori fondiari e aiuto pubblico: possibili effetti sui cambiamenti di uso del suolo

    Directory of Open Access Journals (Sweden)

    Leonardo Casini

    2015-08-01

    Full Text Available Agricultural policies can affect farm economic performances in a such deep manner that sometimes, in the case of absence of public support, this could lead to the termination of production in many rural realities. The objective of this work is to propose a method of assessing the risk of abandonment of agricultural activity that is based on the comparison between the estimated rent and the public aid received by each farms. The methodological approach stems from the inability to detect directly the value of rent and it is based on the elaboration of the database provided by the Agenzia del Territorio on Valori Agricoli Medi (VAM.

  16. La ricerca attuale sui rapporti tra il primo Cristianesimo e la cultura classica alla luce di un recente contributo

    Directory of Open Access Journals (Sweden)

    Ilaria Ramelli

    2005-01-01

    Full Text Available El presente artículo, que se configura principalmente como una amplia recensión, examina las relaciones entre la primera cultura cristiana y la cultura clásica a la luz de un reciente y rico volumen miscelaneo que aporta numerosas e importantes contribuciones al problema y que también proporciona un estado de la cuestión de la investigación en este terreno. El estudio se realiza no sólo desde el contexto del trasfondo cultural griego y romano de las primeras obras cristianas, sino también del eventual conocimiento del primero pensamiento y de los primeros escritos cristianos por parte de los autores paganos.The present review article considers the relationship between the early Christian culture and the classical one in the light of a recent and rich miscellaneous volume that brings many important contributions to this question, and also provides a status quaestionis of the research in this field. It is desirable that scholars investigate not only the Graeco-Roman cultural and philosophical context of the early Christian writings, but also the possible knowledge of the early Christian thought and writings by the pagan authors.

  17. Ma cosa fanno gli americani? Una breve indagine sui ruoli e i compiti dei landscape architect statunitensi (II

    Directory of Open Access Journals (Sweden)

    Danilo Palazzo

    2015-11-01

    Full Text Available American landscape architecture firms seem to have retained between their competences that of city and urban design. To look out how real is this feeling, that refers to what is published on specialized journals and books, the paper display a reconstruction of phases, protagonists, and modalities that characterized the division between the two disciplines, in terms of separate professional associations and academic curriculum. The division appeared to be slow and viscous.The evaluation of city-design-oriented activities conducted by self-defined landscape architecture’s firms is developed since the recognition of the design activities undertook by some of the American firms awarded in 2004 by the ASLA, the American Society of Landscape Architect. 

  18. Implicazioni di Basilea II per la stabilità finanziaria. Le nuvole si addensano sui paesi in via di sviluppo.

    Directory of Open Access Journals (Sweden)

    Mario Tonveronachi

    2007-06-01

    Full Text Available Placing Basel II in the perspective of the more general trend in financial regulation, the paper analyses its efficacy an efficiency as a device ti foster financial resiliency. In assessing the criticism levelled against the New Accord, special attention is devoted to the case of the emerging countries. I suggest that Basel II is neither a sufficient, nor a necessary condition to attain systematic financial stability, especially in weak institutional and macro-policy environments. Taking also into account just how complex and onerous the scheme is, I conclude that the emerging countries should look for new international institutional arrangements based on the principle of a stability level playing field.

  19. Implicazioni di Basilea II per la stabilità finanziaria. Le nuvole si addensano sui paesi in via di sviluppo.

    Directory of Open Access Journals (Sweden)

    Mario Tonveronachi

    2013-06-01

    Full Text Available Placing Basel II in the perspective of the more general trend in financial regulation, the paper analyses its efficacy an efficiency as a device ti foster financial resiliency. In assessing the criticism levelled against the New Accord, special attention is devoted to the case of the emerging countries. I suggest that Basel II is neither a sufficient, nor a necessary condition to attain systematic financial stability, especially in weak institutional and macro-policy environments. Taking also into account just how complex and onerous the scheme is, I conclude that the emerging countries should look for new international institutional arrangements based on the principle of a stability level playing field.  JEL Codes: E44, F53, G21, G28, O16 

  20. Agricultural biotechnologogy patents in Brazil: a critical review

    Directory of Open Access Journals (Sweden)

    Pedro Abel Vieira Junior

    2010-12-01

    Full Text Available The dissemination of knowledge and encouraging inventors to substantiate the emergence of the intellectual property system. The protection of intellectual assets is not neutral in their effects on firms and countries: requirements and costs involved in the search and enforcement of protection have differential impacts across firms and countries, tend to enhance the asymmetry of innovativeness between developed countries. The advance of this debate is essential the establishment of a framework and appropriate stimulator for the development of innovation-based biotechnology in Brazil. The intellectual property on living beings and new biological processes is one of the most controversial aspects of the process of setting up international markets and the “modernization” of the economy. This is an extremely controversial issue in which there are many issues unresolved and subject to a heated debate involving conflicting interests in economic, political, social, cultural and technical, not to speak on moral and religious aspects, equally important. In this context, this work aims to make a conceptual analysis of terms used in biotechnology, making an interpretation of provisions in the law that governs the rights and obligations relating to industrial property. It is opposed to the law of protection of cultivars, establishing intellectual property protection sui generis, analyzing what are the main economic consequences that may result from mistaken interpretation of legislation.A difusão do conhecimento e o estímulo aos inventores fundamentaram o surgimento do sistema de propriedade intelectual. A proteção aos ativos intelectuais não é neutra em seus efeitos sobre empresas e países: exigências e custos envolvidos na busca e no enforcement da proteção têm impactos diferenciados entre empresas e países e tendem a reforçar a assimetria de capacidade de inovação entre países desenvolvidos. O avanço deste debate é essencial para o

  1. “Vistiendo las ropas del santo”: Atributos afro en la personalidad de san Baltasar a través de algunos cargos devocionales en su culto en la Argentina

    Directory of Open Access Journals (Sweden)

    Norberto Pablo Cirio

    2003-12-01

    Full Text Available Through its history, one can see traces of African roots in the popular cult of San Baltazar in Argentina. In spite of starting as an imposed devotion by the clergy and the Spanish Crown to the slaves in the middle of 18th Century, early the later could insert their own cultural traditions, producing a sort of sui generis syncretism.Now days, those traditions are present in some religious ceremonies, especially in the devotional area. So far they consider that he is the patron saint of the joy and fun, they celebrate him with music and dance, being some of them from black roots. In this paper I wish to analyze one of these religious principles. So far there isn't any difference between kings and goods, the divine personality has a bipolar behavior that comes to real in a unique entity movable-non movable, thing- human. In this way, being goods also kings and kings also goods, their divine and royal attributes are object of devotion and subordination by their faithful people-subjects. My hypothesis is that that black people could have applied this principle so far they knew that double characteristic of this saint: divine (he is a saint and royal (he is one of the three Wise Men, compatible with their ancestral believes.//Al revisar la historia del culto popular de San Baltazar en Argentina, es posible encontrar sus raíces africanas. A pesar de haber empezado como una devoción impuesta por el clero y la Corona española a mediados del siglo XVIII , los esclavos lograron insertar sus propias tradiciones culturales, produciendo así una suerte de sincretismo sui generis.Hoy en día, dicha tradición está presente en celebraciones religiosas, especialmente en el área devocional. A San Baltazar se le considera el patrón de la alegría y la diversión y sus celebraciones se realizan con música y bailes, algunos de ellos de raíces negras.En este trabajo me propongo analizar uno de los principios de este culto. Como no hay diferencias entre los

  2. Doença mental, raça e sexualidade nas teorias psiquiátricas de Juliano Moreira Mental disease, race, and sexuality in the psychiatric theories of Juliano Moreira

    Directory of Open Access Journals (Sweden)

    Ana Teresa A. Venancio

    2004-07-01

    Full Text Available Tomando como ponto de partida a representação erudita que situa Juliano Moreira como fundador da psiquiatria científica no Brasil, este trabalho analisa as concepções por ele veiculadas sobre doença mental, raça e sexualidade. Suas teorias constituíram uma transposição sui generis do pensamento do psiquiatra alemão Emil Kraepelin, ajudando na construção do ideário em prol do processo civilizatório brasileiro no início do século XX. Fundamentava-se em pressupostos organicistas, ao mesmo tempo em que se contrapunha à idéia corrente na época sobre as condições "naturais" brasileiras desfavoráveis como o clima e a raça. Analisando os trabalhos publicados por Juliano Moreira até 1920, relaciona-se aqui o modo como seu discurso científico discutia o tema da sexualidade, expressa sob a rubrica da "sífilis", da "reprodução" e do "casamento". Com relação a essa temática, Juliano Moreira não se valeu da idéia de uma natureza feminina degenerada, crescentemente difundida em meados do século XIX. Ainda que não desconsiderasse a idéia mais geral da degeneração, pertinente apenas ao nível das unidades orgânicas individuais, descartaria a correlação entre degeneração e natureza feminina, para se ater aos fundamentos médicos hegemônicos na primeira metade do século XIX, que articulavam as marcas diferenciais da mulher à sua fisiologia sexual, paradigmaticamente representada pela imagem do útero.Taking as the point of departure the academic image of Juliano Moreira as the founder of scientific psychiatry in Brazil, this study analyzes the concepts he expressed on mental disease, race, and sexuality. His theories constituted a sui generis transposition of the thinking of German psychiatrist Emil Kraepelin, aiding in the construction of the ideals in defense of the Brazilian civilizational process in the early 20th century. He based his concepts on organicist premises, while arguing against the then

  3. Antithetic Foundations of Economics

    Directory of Open Access Journals (Sweden)

    Marin Dinu

    2011-03-01

    Full Text Available This paper aims at decrypting the manner in which the foundations of Economics as a science and the meanings of the relevant explanatory formulas are being shaped. My analytical endeavor focuses on understanding the peculiarities of what is referred to as the object of study of the science known as Economics, an academic synthesis of concept-related breakthroughs regarding economicity. The explicit purpose of this analysis is to identify perennial benchmarks in economic cognition whereby this ensures its consistency. The implicit purpose is to shape a cognitive model in line with the specifics of the conceptual universe of Economics, as well as with the sources of the economic realities that are subject to a sui-generis relativism. The primary benefit of this endeavor consists in systemizing the conceptual prospects with an antithetic nature that allow for the explanations of the state of economic rationality and generate the understanding of what the source of economicity is and how it behaves. As such, the conclusions are marked by the stringent need of more precisely defining economic knowledge in order to match the changing nature of economic reality, as an expression that embraces the meeting point of two ontological vistas that are methodologically separated by some theories: human nature and human condition. Economics as a science thus features, apart from a conceptual substrate that needs to be spotted, an ontological background that needs to be revealed. The role played by this background appears to be most frequently ignored. The joint identification of both direct and contextual determinants for a sensitive area of humankind, i.e. the economy, is a direction to be followed by the royal path of rational knowledge.

  4. McDowell e as estátuas de Dédalo

    Directory of Open Access Journals (Sweden)

    João Carlos Brum Torres

    Full Text Available ABSTRACT The subject of this paper is the concept of 'demonstrative concept', which has been introduced in philosophy by John McDowell in Mind and World. The analysis presented in the article has a reconstructive and a critical character. Its reconstructive drive may be viewed as required by the concise mode the philosopher adopted to present his theoretical innovation and comprehends an effort to make clear: (i what is the difference between the temporal conditions necessary (a to give conceptual status to the color shade we have before our eyes and (b to our possession of ordinary concepts; (ii the sui generis character of the universality proper to demonstrative concepts and the uniformity of their relations to what they are concepts of; (iii the equivalence of their semantical regime to that which is proper to concepts of measurement standards; (iv the very singular function performed by memory in the constitution and possession of demonstrative concepts. Now, the critical character of the paper firstly lies in the remark that there is a kind of inconsequence in the way McDowell conceives the employment of those concepts, namely in his refusal to admit that even though it is natural to say 'I see the shade in my mind's eye', it would be a mistake to think that when we recognize a color shade as being the same as the one we have seen before there is an inner comparison of it with the retained sample. The second of the paper critical points is the warning that, considering the complex constitution process of demonstrative concepts and its exacting semantic, it seems impossible to take them as coextensive to our human experience of colors.

  5. Assessment of Cultivar Distinctness in Alfalfa: A Comparison of Genotyping-by-Sequencing, Simple-Sequence Repeat Marker, and Morphophysiological Observations

    Directory of Open Access Journals (Sweden)

    Paolo Annicchiarico

    2016-07-01

    Full Text Available Cultivar registration agencies typically require morphophysiological trait-based distinctness of candidate cultivars. This requirement is difficult to achieve for cultivars of major perennial forages because of their genetic structure and ever-increasing number of registered material, leading to possible rejection of agronomically valuable cultivars. This study aimed to explore the value of molecular markers applied to replicated bulked plants (three bulks of 100 independent plants each per cultivar to assess alfalfa ( L. subsp. cultivar distinctness. We compared genotyping-by-sequencing information based on 2902 polymorphic single-nucleotide polymorphism (SNP markers (>30 reads per DNA sample with morphophysiological information based on 11 traits and with simple-sequence repeat (SSR marker information from 41 polymorphic markers for their ability to distinguish 11 alfalfa landraces representative of the germplasm from northern Italy. Three molecular criteria, one based on cultivar differences for individual SSR bands and two based on overall SNP marker variation assessed either by statistically significant cultivar differences on principal component axes or discriminant analysis, distinctly outperformed the morphophysiological criterion. Combining the morphophysiological criterion with either molecular marker method increased discrimination among cultivars, since morphophysiological diversity was unrelated to SSR marker-based diversity ( = 0.04 and poorly related to SNP marker-based diversity ( = 0.23, < 0.15. The criterion based on statistically significant SNP allele frequency differences was less discriminating than morphophysiological variation. Marker-based distinctness, which can be assessed at low cost and without interactions with testing conditions, could validly substitute for (or complement morphophysiological distinctness in alfalfa cultivar registration schemes. It also has interest in sui generis registration systems aimed at

  6. Consumo, ética e natureza: o veganismo e as interfaces de uma política de vida

    Directory of Open Access Journals (Sweden)

    Aline Trigueiro

    2013-06-01

    Full Text Available http://dx.doi.org/10.5007/1807-1384.2013v10n1p237 Este trabalho busca refletir sobre um tipo de ativismo que está ganhando amplitude, embora de modo silente, e que integra no corpo de suas reivindicações uma proposta ousada de ressignificação da relação entre humanos e animais: trata-se do veganismo. Em linhas gerais, o veganismo almeja a construção de um postulado ético que reconheça os direitos dos animais e a inclusão dos mesmos à comunidade moral, e um modus vivendi que projeta para o centro do debate uma responsabilidade para com o ato de consumo (mudanças de estilo de vida e de hábitos alimentares. Estes são os aspectos analisados neste artigo, os quais são investigados a partir de um viés teórico e também de uma abordagem empírica: pesquisas realizadas em alguns sites da internet, por meio de um mapeamento e investigação dos grupos reconhecidamente veganos e dos fóruns direcionados à discussão sobre os direitos dos animais e o veganismo. Acrescenta-se que a escolha de tal objeto de pesquisa deve-se ao fato de percebermos, na constituição e na proposta dessa forma de ativismo, uma manifestação social sui generis, reveladora de como diferentes grupos sociais estão respondendo aos dilemas que conformam e impactam a sociedade hodierna, principalmente no que concerne ao posicionamento ético: como devemos viver?

  7. Estratégias de mediação cultural: Inovação e experimentação no Serviço Educativo da Casa da Música

    Directory of Open Access Journals (Sweden)

    Pedro Quintela

    2012-10-01

    Full Text Available O tema da mediação cultural readquiriu nas três últimas décadas muita relevância nos discursos políticos e programáticos que apelam à formação e atração de públicos para as artes e a cultura. Este apelo, muito associado ainda aos princípios da “democratização cultural”, traduz igualmente as preocupações de sustentabilidade sentidas por agentes e instituições culturais, num contexto em que o poder público tende a desvincular‑se do financiamento à cultura. Em Portugal, estas preocupações vêm suscitando o desenvolvimento dos chamados “serviços educativos” e de novas estratégias de mediação cultural em múltiplas instituições e equipamentos. Este artigo toma como objeto o Serviço Educativo da Casa da Música e analisa os contornos sui generis de alguns projetos aí desenvolvidos. Procura compreender o modo como esses projetos incorporam uma dimensão “experimental” nas formas de relacionamento com públicos e no desenvolvimento de novos modos de promover a aproximação às práticas criativas no campo da música. Discutem‑se ainda tendências de mudança mais amplas nas estratégias de mediação cultural em organizações artísticas, de que o caso estudado é ilustrativo.

  8. Triple play service under the impact of nonstationary noise in a DSL system: an Amazon approach

    Science.gov (United States)

    de Souza, Lamartine V.; Cardoso, Diego; Silva, Marcelino; Seruffo, Marcos; Francês, Carlos R. L.; Costa, João C. W. A.; Castro, Agostinho L. S.; Cavalcante, Gervásio; Rius i Riu, Jaume

    2007-09-01

    The Brazilian Amazon has sui generis characteristics that affect strongly the communication technologies, such as high humidity and temperature. These characteristics cause impact in the existent infrastructure, especially in twisted-pair copper lines. At the moment, new services are based on multimedia applications, as voice over internet protocol (VoIP), video on demand (VoD), and internet protocol television (IPTV). Such services use digital broadband networks such as ADSL2+ (Asymmetric Digital Subscriber Line) to transmit the data. Broadband services require data rates which can only be achieved by using relatively high spectrum frequencies. At high frequencies the DSL signal is more susceptible to external noise sources, such as radio frequency interference and impulsive noise. For this purpose, an experimental setup has been built at UFPA that consists of noise generator, traffic generator, real cables, modems and DSLAM (digital subscriber line access multiplexer). This paper aims at characterizing how the noise impacts on triple play services for a broadband system using a DSL loop on Amazon environment. The objective of the noise impact experimentation is to observe the behavior of a DSL system under more realistic but controlled line conditions. Metrics as lost packet, jitter, latency, and throughput are used to characterize the triple play service in a DSL loop under the noise impact. Through the real experiments and controlled loop conditions, this paper allow identify, from application level point of view, how robust DSL system is in respect to noise occurrence. Additionally, it is described a methodology for noise impact measurements using a DSL system.

  9. O futuro anterior: continuidades e rupturas nos movimentos estudantis do Brasil

    Directory of Open Access Journals (Sweden)

    Breno Bringel

    2009-01-01

    Full Text Available Durante o ano de 2008, multiplicaram-se, em todo o mundo, as reflexões sobre as lutas estudantis motivadas pelo 40º aniversário do Maio de 1968. No caso brasileiro, a questão mais paradigmática não foi tanto a discussão das lutas da década de 1960, mas sua "atualização" no contexto nacional, tendo como base possíveis rupturas que as lutas recentes dos estudantes universitários brasileiros, com vários protestos e ocupações entre 2007 e 2008, podem provocar no cenário dos movimentos estudantis no Brasil. Uma das hipóteses centrais propostas neste artigo é que esses movimentos se manifestam de acordo com dinâmicas cíclicas, e pela retroalimentação com outros movimentos sociais. Para observar essa dinâmica de continuidade e ruptura propõem-se alguns elementos para uma abordagem teórico-metodológica que interprete os movimentos estudantis como um movimento social sui generis. Realizase também uma análise cíclica das ações coletivas estudantis no Brasil e se avaliam, em perspectiva histórica e comparada com as dinâmicas de outros movimentos sociais, as principais características das lutas dos movimentos estudantis contemporâneos no País.

  10. THE RELATION BETWEEN TERRITORIAL COLECTIVITIES IN FRANCE AND THE EUROPEAN UNION. THOUGHTS ON THE CROSS-BORDER COOPERATION

    Directory of Open Access Journals (Sweden)

    CLAUDIA BARBOIU-GILIA

    2012-05-01

    Full Text Available France, one of the founding members of the European Union, is a unitary state from the administrativeterritorial point of view, based on deep centralism. Having territorial collectivities with highly complex structure (communes, departments, regions, sui-generis collectivities and overseas collectivities, France committed itself to cooperation not only between its own administrative structures, but also to cross-border cooperation within the European Union. After showing reluctance to external actions underwent by territorial collectivities, France ended up with acknowledging this right of its territorial collectivities within the «decentralized cooperation», expressly brought under regulation by the Law of 6 February 1992. According to the law, there is no need for any ratification on behalf of the State to allow cooperation between territorial collectivities, within the boundaries of their competence. The Law of 1992 thus authorized the territorial collectivities to close agreements with other collectivities from abroad. Furthermore, the Law of 4 February 1995 allowed several treaties with the border states to be signed, thus creating the SAAR-LOR-LUX region (an European cross-border region that made way for cooperation between Germany, France and Luxembourg. The French legislation also allowed several European districts to be created, acting as local groups for cross-border cooperation, created on the initiative of territorial collectivities. The aim of our study is to identify the main relationship between territorial collectivities in France and EU and to analyze the cooperation instruments used by the French collectivities in order to foster the cross-border cooperation.

  11. A theoretical model for estimating the vacancies produced in graphene by irradiation

    International Nuclear Information System (INIS)

    Codorniu Pujals, Daniel; Aguilera Corrales, Yuri

    2011-01-01

    The award of the Nobel Prize of Physics 2010 to the scientists that isolated graphene is a clear evidence of the great interest that this system has raised among the physicists. This quasi-two-dimensional material, whose electrons behave as massless Dirac particles, presents sui generis properties that seem very promising for diverse practical applications. At the same time, the system poses new theoretical challenges for the scientists of very different branches, from Material Science to Relativistic Quantum Mechanics. A topic of great actuality in graphene researches is the search of ways to control the number and distribution of the defects in its crystal lattice, in order to achieve certain physical properties. One of these ways can be the irradiation with different kind of particles. However, the irradiation processes in two-dimensional systems have been insufficiently studied. The classic models of interaction of the radiation with solids are based on three-dimensional structures, for what they should be modified to apply them to graphene. In the present work we discuss, from the theoretical point of view, the features of the processes that happen in the two-dimensional structure of monolayer graphene under irradiation with different kinds of particles. In that context, some mathematical expressions that allow to estimate the concentration of the vacancies created during these processes are presented. We also discuss the possible use of the information obtained from the model to design structures of topological defects with certain elastic deformation fields, as well as their influence in the electronic properties. (Author)

  12. Spectroscopic Comparison of Metal-rich RRab Stars of the Galactic Field with their Metal-poor Counterparts

    Energy Technology Data Exchange (ETDEWEB)

    Chadid, Merieme [Université Nice Sophia–Antipolis, Observatoire de la Côte dAzur, UMR 7293, Parc Valrose, F-06108, Nice Cedex 02 (France); Sneden, Christopher [Department of Astronomy and McDonald Observatory, The University of Texas, Austin, TX 78712 (United States); Preston, George W., E-mail: chadid@unice.fr, E-mail: chris@verdi.as.utexas.edu, E-mail: gwp@obs.carnegiescience.edu [Carnegie Observatories, 813 Santa Barbara Street, Pasadena, CA 91101 (United States)

    2017-02-01

    We investigate atmospheric properties of 35 stable RRab stars that possess the full ranges of period, light amplitude, and metal abundance found in Galactic RR Lyrae stars. Our results are derived from several thousand echelle spectra obtained over several years with the du Pont telescope of Las Campanas Observatory. Radial velocities of metal lines and the H α line were used to construct curves of radial velocity versus pulsation phase. From these we estimated radial velocity amplitudes for metal lines (formed near the photosphere) and H α Doppler cores (formed at small optical depths). We also measured H α emission fluxes when they appear during primary light rises. Spectra shifted to rest wavelengths, binned into small phase intervals, and co-added were used to perform model atmospheric and abundance analyses. The derived metallicities and those of some previous spectroscopic surveys were combined to produce a new calibration of the Layden abundance scale. We then divided our RRab sample into metal-rich (disk) and metal-poor (halo) groups at [Fe/H] = −1.0; the atmospheres of RRab families, so defined, differ with respect to (a) peak strength of H α emission flux, (b) H α radial velocity amplitude, (c) dynamical gravity, (d) stellar radius variation, (e) secondary acceleration during the photometric bump that precedes minimum light, and (f) duration of H α line-doubling. We also detected H α line-doubling during the “bump” in the metal-poor family, but not in the metal-rich one. Although all RRab probably are core helium-burning horizontal branch stars, the metal-rich group appears to be a species sui generis.

  13. Cuba y el Socialismo del siglo XXI

    Directory of Open Access Journals (Sweden)

    Iván Porfirio Santos Víctores

    2017-12-01

    Full Text Available El presente artículo trata acerca de cómo, después de la desaparición  del socialismo en la URSS y en Europa del Este, el llamado Socialismo del siglo XXI representa el desafío actual de reinventar o renovar el socialismo; tomando en cuenta las experiencias de los socialismos que se vivieron, aunque partiendo siempre de las condiciones y la realidad concreta y particular de cada país. Cuando se habla de Socialismo del Siglo XXI no se trata sobre algo definitivamente acabado, conceptualizado, sino precisamente sobre un constructo en desarrollo, el cual es la expresión actual de un abordaje renovado de la construcción del socialismo. Cuba, que inició su proceso revolucionario a mediados del siglo pasado, constituye, con su proyecto de actualización del socialismo, un ejemplo paradigmático donde, con sus características y particularidades históricas, encontramos lo que podríamos llamar una transición del socialismo clásico alternativo sui generis del siglo XX al  socialismo del siglo XXI, con la adopción de políticas públicas que estimulen la sostenibilidad del desarrollo económico y social del país mediante la consolidación de un socialismo próspero y sostenible, comprometido con los valores fundacionales  históricos del proyecto social cubano.

  14. TURKEY’S ACCESSION TO THE EUROPEAN UNION – PRESENT OR PERSPECTIVES?

    Directory of Open Access Journals (Sweden)

    Ileana Voica

    2017-12-01

    Full Text Available The enlargement of the European Union is a sui generis process, which involves internal preparation of the candidate countries, the European Union and accession negotiations as well. The EU enlargement process is based on the desire to create a close relationschip between the European countries in a common economic and political project. Guided by tha values of the European Union and subject to strict conditions, the enlargement proved to be one of the most effective tools for promoting political, economic and social reforms and to strengthen pceace, stability and democracy across the continent. A controversial topic in the last period is Turkey’s accession to the European Union. Turkey’s European ambitions date back to the 1963 Ankara Agreements, although it has formally submitted the membership application in 1987. Following the Helsinki European Council of 10 – 11 December 1999, accession negotiations between the EU and Romania, Latvia, Lithuania, Slovakia, Malta and Bulgaria started on 15 February 2000. Regarding the accession negotiations with Turkey, il was considered that this country does not meet, at this stage, the criteria set by the Copenhagen European Council on the rule of law, democracy and human rights. For Turkey, an Accession Partnership was adopted on 8 March 2001. Currently, Turkey is far from concluding the process of joining the European Union. However, Turkey is a state that can no longer be ignored by anyone in the world politics, being remarked by the infrastructure projects, the developed tourism, the steady economic growth and, last but not least, by the impressive military power, being the second NATO army. Given that, in the framework of the enlargement process, both the candidate states and the old members must be prepared for integration and cohabitation as well as the negative opinion on the Turkish membership of the influential states, such as Germany and France, it remains to be seen whether Turkey will

  15. Cherubism: Clinicoradiographic Features and Treatment

    Directory of Open Access Journals (Sweden)

    Luiz Antonio Guimarães Cabral

    2010-04-01

    Full Text Available Objectives: Cherubism is a congenital childhood disease of autosomal dominant inheritance. This disease is characterized by painless bilateral enlargement of the jaws, in which bone is replaced with fibrous tissue. The condition has sui generis clinical, radiographic and histological features, of which the clinician should be aware for a better differential diagnosis in the presence of a fibro-osseous lesion affecting the bones of the maxillomandibular complex. The purpose of present paper was to review the literature and to report the most important aspects of cherubism in order to facilitate the study of this disease.Material and Methods: Literature was reviewed about cherubism, emphasizing the relevant clinicoradiographic features and treatment. Literature was selected through a search of PubMed and Scielo electronic databases. The keywords used for search were adolescent, cherubism, cherubism/physiopathology, cherubism/treatment, cherubism/radiography. A manual search of the reference lists of the identified articles and the authors’ article files and recent reviews was conducted to identify additional publications. Those studies that described new features about cherubism were included in this review.Results: In total 44 literature sources were obtained and reviewed. Studies that described new features about cherubism physiopathology, diagnostics and treatment were reviewed.Conclusions: Despite the exceptions, cherubism is a clinically well-characterized disease. In cases of a suspicion of cherubism, radiographic examination is essential since the clinical presentation, the location and distribution of the lesions may define the diagnosis. Histopathological examination is complementary. Nowadays, genetic tests should be used for final diagnosis of cherubism.

  16. Imaginario femenino en el populismo argentino. Género y nación en La razón de mi vida, de Eva Perón

    Directory of Open Access Journals (Sweden)

    Susana Rosano

    2014-06-01

    Full Text Available En La razón de mi vida, Eva Perón ayuda a construir una imagen de sí misma profundamente atravesada por estrategias de representación melodramáticas. En contraste con la mayoría de las autobiografías femeninas o masculinas, no se trata aquí de un recuento introspectivo. Eva no relata su vida desde el comienzo, desde su infancia. No aprovecha la oportunidad para echar luz sobre las zonas oscuras de su biografía ni para aclarar ninguna de las acusaciones a las que sus reconocidos “supercríticos” la tenían acostumbrada. Se trata, por lo tanto, de una autobiografía sui generis, descentrada, híbrida: el relato de los años de lucha política junto a Perón. De esta manera, Eva se inscribe en el discurso político con una estrategia absolutamente inusual para el género. Su participación en la arena pública está registrada desde un desvío: su lucha es una historia de amor múltiple, con Perón y con “el pueblo”. El objetivo de este trabajo es analizar las tecnologías autobiográficas utilizadas en el texto en relación con los discursos de verdad e identidad femenina disponibles en la época en que fue escrito, la década del 50 en Argentina.

  17. La protección del software en Cuba. Comparación con otros países latinoamericanos.

    Directory of Open Access Journals (Sweden)

    Nerbys Hernández Dorta

    2009-10-01

    Full Text Available En los últimos años los asuntos relacionados con la Propiedad Intelectual han ido cobrando importancia, por lo que la legislación en ese sentido ha tenido que sufrir mutaciones que la han llevado a la búsqueda de estructuras que le permitan desarrollarse en toda su extensión. El proceso de digitalización que se diversificó con la aparición de la Sociedad de la Información, entrañó la aparición de una nueva categoría de obras que se debían insertar en las concepciones de los Derechos de Propiedad Intelectual, pues aparecían bienes intangibles con una naturaleza diferente a los ya conocidos, entre ellos el software, el cual ha sido objeto de un polémico debate en cuanto a la necesidad de su protección. Desde su creación se ha tratado de determinar una figura que le diera amparo legal, y para ello siempre se mantuvo en el derecho de las invenciones, el derecho de autor y una protección sui generis. Es por ello que se realizó una revisión de la legislación existente en la materia para de forma concreta proporcionarles información a los interesados en el tema sobre la protección legal de los programas de ordenador en Cuba; así como su comparación con legislaciones en esta materia en países como Bolivia y Venezuela.

  18. European social model and the harmonization of Macedonian labour legislation with the European Union community labour law

    Directory of Open Access Journals (Sweden)

    Kalamatiev Todor

    2014-01-01

    Full Text Available The European integration process accompanied by the harmonization of the EU member states' legislation will inevitably lead to the establishment of a European social model which has a 'sui generis' legal nature. European social model forms a part of the common legal heritage of the European countries and it can be defined as a system of values that include democracy and individual rights, free collective bargaining, market economy, equal opportunities for all, and social protection and solidarity. Subject of analysis in this paper are both, the essential components of the European social model such as: the social 'acquis communautaire' and the European industrial relations. The social 'acquis communautaire' is an integral part of the general 'acquis communautaire' and it encompasses the regulations arising from the primary and secondary labour law legislation of the EU, decisions of the European Court of Justice and other legal measures of the European Union with a binding or non-binding legal nature. European industrial relations encompass the key features of the European trade union models as well as the types of social dialogue in different EU member states. This paper pays attention to a profound elaboration of the harmonization of two EU labour law directives with the Macedonian employment legislation. The first Directive (Directive 91/533/EEC of 14 October 1991 on an employers' obligation to inform employees of the conditions applicable to the contract or employment relationship may be classified in the field of individual employment relations, while the second one (Directive 2002/14/EC of 11 March 2002 establishing a general framework for informing and consulting employees in the European Community can be subsumed within the field of collective employment relations. Finally, the authors illustrate the advantages and disadvantages of the Macedonian labour legislation in the process of harmonization with the relevant EU directives

  19. What are 'Think Tanks'? Revisiting the Dilemma of the Definition

    Directory of Open Access Journals (Sweden)

    Juliana Cristina Rosa Hauck

    2017-08-01

    Full Text Available 'Think tanks' (TTs are 'sui generis' organizations that have been proliferating, playing more and more the role of very relevant actors on the political scene in clashes over interests, preferences and ideas for the formation of public policies. The definition of 'think tank', however, creates a conceptual gap that causes serious limitations on the development of this field of study. Excessive generality or inflexibility in the use of the concept raises questions about the validity of the category. Either way, the 'trade offs' of the conceptual choices have delineated a not very useful concept of think tank to both inter-contextual and inter-organizational comparability and to differentiate think thanks from overlapping organizations. The article revisits some of these dilemmas. To do so, the treatment in the specialized literature of the analytic category, highlighting both the insensitivity of the traditional North American matrix to different national contexts and its obsolescence to the changes in the context of TTs' action, including their own context of origin, is explained. In shedding light on promising conceptual contributions, the main contribution of the present article is the proposal of the radial structuring of the concept of think tanks as 'boundary spanners', as advanced by Medvetz, and of corresponding indicators to deal with the large variation in the substantive properties of the operationalization of the adopted concept. Thus, the aim is to reduce non-specificities in the analytic category and the dissent of its social significance, which demands less abstraction facing the exploratory state of the field of study.

  20. Transvestites: bodies in transit? Dreams, myth and reality

    Directory of Open Access Journals (Sweden)

    Maria Alves de Toledo Bruns

    2016-05-01

    Full Text Available The goal of this article is to discuss the phenomenon of the ethos of affective sexual experience of transvestites at the interface with modeling one’s body. A changing reality, the body in contemporary society is a kind of merchandise - a product entitled to minor repairs and predetermined duration. It was also observed the construction of identities and subjectivities based on artifacts established under or on the skin: piercings, tattoos, scarification, silicone, hormones, body building, crossdressing – which have turned the body into a means of affirmation of the self. The use of silicone, hormones and plastic surgery to achieve the ideal body modeling by transvestites has become commonplace among this population leading to an issue of public health due to the injuries, deformities and even death that often result from such practices. The phenomenological method was chosen to analyze, understand and interpret the speeches of three transvestites belonging to social classes C and D; aged between 20 and 30 years on the meanings they attribute to the process of modeling their body and the affective sexual practices arising from such transformation. The results obtained from the analysis of the three interviews led to three categories: "The dream of beauty and a perfect body"; "Pain and the recovery process as a rite of passage"; and "Change in behavior / personality resulting from the applications". In their search of the perfect body they dare and cross the ethical boundaries of aesthetics and health. They subvert the sense of self-care to meet the market logic. The transvestites mutilate and shape their bodies; they shape a sui generis body that seeks balance between the feminine and the masculine and also claims the existence of a particular ethos.

  1. Researchers and experts faced with legal issues in radon affairs

    International Nuclear Information System (INIS)

    Massuelle, M.; Pirard, P.; Hubert, P.

    1998-01-01

    In France, radon has emerged as a public health issue mainly at the initiative of scientists. Public authorities are currently considering regulations but for a long time scientists faced the radon issue alone. As a consequence, scientists were involved and are still involved in producing knowledge, in informing about their results, in giving advice to various bodies and individuals, and in participating in the process of technical standardization. These functions are identified in the paper in order to sketch out a typology of different situations, formal and informal, in which researchers transformed into experts are called to collaborate. During their missions related to radon, experts are exposed to 'legal risks', particularly in terms of civil liability or 'professional' responsibility and even criminal responsibility. They face legal difficulties because their roles are not clearly defined. Such difficulties will be also described in this paper, because they are symptomatic of the lack of a legal framework for public scientific expertise. Indeed, there is a growing need to involve scientific experts in decision-making in the field of public health. At the same time, however, there is increased protest against the technocratic nature of public decision-making. We observe an increase in the attribution of blame and penal responsibility in French society, as shown in the 'contaminated blood' case in which not only blood suppliers but also public officials and now politicians have been or are being prosecuted. Radon, which is a domestic risk whose reduction relies entirely on homeowners, is sui generis in many ways. Nevertheless, in an analysis of scientists' roles/actions and of the legal difficulties they meet, radon can be used to illustrate the problems that arise as expertise is developed about new risks. (authors)

  2. La concepción unamuniana de asignatura como ciencia viva

    Directory of Open Access Journals (Sweden)

    Emanuel José Maroco-dos-Santos

    2017-01-01

    Full Text Available Para Unamuno, que concibe al catedrático como un “fraguador de doctrinas”, la asignatura universitaria, ubicada en las antípodas de su concepción en la enseñanza secundaria, debería ser una expresión de una “ciencia viva” o in fieri, y no un repositorio de conocimientos. Con dicha toma de posición, el rector salmantino terminó por oponerse a la ciencia oficial y al dogma científico a favor de una enseñanza de procedimientos. El presente trabajo procura recuperar dicha concepción de asignatura, intentando desvelarla en sus varias dimensiones con vistas a aportar al espacio de la reflexión pedagógica contemporánea las sui generis ideas educativas unamunianas que, por su densidad y sentido, permiten enfocar la asignatura académica no como un espacio de mera instrucción, sino como un momento de investigación de “hipótesis y utopías”. La gran propuesta unamuniana es, pues, la de que docente y estudiante pierdan sus características clásicas, que les definen como un “templo del saber” y un “botijo vacío”, respectivamente, a favor de una concepción más adaptada a los nuevos tiempos académicos, en que el perenne desarrollo científico y cultural hace obsoletos los contenidos asimilados ayer.

  3. THE COURT OF JUSTICE OF THE EUROPEAN UNION AND INTERNATIONAL LEGAL ORDER

    Directory of Open Access Journals (Sweden)

    TETYANA KOMAROVA

    2017-01-01

    Full Text Available The author discusses the relationship between two legal orders: international law and European Union (EU law. The main provisions of this relationship have been established through the precedential practice of the Court of Justice of the European Union – the EU’s main judicial body. This kind of research seems important because of the gap in the theory of international law caused by the immutable dogma of the supremacy of international law. However, modern legal practice demonstrates a certain fragmentation of the international legal order because of the impact of the existence and development of regional supranational legal orders. The EU legal order, with its own special nature (sui generis, is undoubtedly one of the most developed among them. The Court of Justice of the European Union performs a crucial role in the EU legal system concerning application and interpretation of EU law. It provides a uniform interpretation of this law for the purposes of development of supranational integration. In this context the Court of Justice the European Union establishes the status of European law and its relationship with the national legal systems and international law. The Court acts as protector of the EU legal order against the influence of other legal orders. The Court’s precedential practice reveals EU law’s tendency towards its constitutionalization and the development of its autonomy. The latest practice indicates the Court’s powers to review the EU institutions’ acts in relation to the implementation of UN Security Council resolutions. This proves the Court’s ability to establish indirect control even over UN acts.

  4. "A disease of our time": The Catholic Church's condemnation and absolution of psychoanalysis (1924-1975).

    Science.gov (United States)

    Foschi, Renato; Innamorati, Marco; Taradel, Ruggero

    2018-03-01

    The present paper is focused on the evolution of the position of the Catholic Church toward psychoanalysis. Even before Freud's The Future of an Illusion (1927), psychoanalysis was criticized by Catholic theologians. Psychoanalysis was viewed with either contempt or with indifference, but nonpsychoanalytic psychotherapy was accepted, especially for pastoral use. Freudian theory remained for most Catholics a delicate and dangerous subject for a long time. From the center to the periphery of the Vatican, Catholic positions against psychoanalysis have varied in the way that theological stances have varied. In the middle decades of the twentieth century, some Catholics changed their attitudes and even practiced psychoanalysis, challenging the interdict of the Holy Office, which prohibited psychoanalytic practice until 1961. During the Cold War, psychoanalysis progressively became more and more relevant within Catholic culture for two main reasons: changes in psychoanalytic doctrine (which began to stress sexuality to a lesser degree) and the increasing number of Catholic psychoanalysts, even among priests. Between the 1960s and the 1970s, psychoanalysis was eventually accepted and became the main topic of a famous speech by Pope Paul VI. This paper illustrates how this acceptance was a sort of unofficial endorsement of a movement that had already won acceptance within the Church. The situation was fostered by people like Maryse Choisy or Leonardo Ancona, who had advocated within the Church for a sui generis use of psychoanalysis (e.g., proposing a desexualized version of Freudian theories), despite warnings and prohibitions from the hierarchies of the Church. © 2018 Wiley Periodicals, Inc.

  5. Non-perturbative renormalization on the lattice

    International Nuclear Information System (INIS)

    Koerner, Daniel

    2014-01-01

    Strongly-interacting theories lie at the heart of elementary particle physics. Their distinct behaviour shapes our world sui generis. We are interested in lattice simulations of supersymmetric models, but every discretization of space-time inevitably breaks supersymmetry and allows renormalization of relevant susy-breaking operators. To understand the role of such operators, we study renormalization group trajectories of the nonlinear O(N) Sigma model (NLSM). Similar to quantum gravity, it is believed to adhere to the asymptotic safety scenario. By combining the demon method with blockspin transformations, we compute the global flow diagram. In two dimensions, we reproduce asymptotic freedom and in three dimensions, asymptotic safety is demonstrated. Essential for these results is the application of a novel optimization scheme to treat truncation errors. We proceed with a lattice simulation of the supersymmetric nonlinear O(3) Sigma model. Using an original discretization that requires to fine tune only a single operator, we argue that the continuum limit successfully leads to the correct continuum physics. Unfortunately, for large lattices, a sign problem challenges the applicability of Monte Carlo methods. Consequently, the last chapter of this thesis is spent on an assessment of the fermion-bag method. We find that sign fluctuations are thereby significantly reduced for the susy NLSM. The proposed discretization finally promises a direct confirmation of supersymmetry restoration in the continuum limit. For a complementary analysis, we study the one-flavor Gross-Neveu model which has a complex phase problem. However, phase fluctuations for Wilson fermions are very small and no conclusion can be drawn regarding the potency of the fermion-bag approach for this model.

  6. Literary translation between Italian and English. Publishing trends in Italy, the UK and the USA

    Directory of Open Access Journals (Sweden)

    Maria Elisa Fina

    2015-12-01

    Full Text Available Abstract - This study aims to investigate publishing trends in literary translation from English into Italian in Italy and from Italian into English in the UK and the USA over the 2000-2008 time span. The data were retrieved from the Index Translationum, an international bibliography of translations managed by UNESCO, and were then stored in three Microsoft Access databases, one for each country. The databases were analysed according to specific criteria, in order to determine trends concerning the amount of translated literature in the three countries, the main publishing houses dealing with translated works, and the most translated genres and authors. This paper is meant to provide a mainly descriptive, but necessary basis for future investigation of socio-cultural as well as market-led processes underlying publishers’ choices and gate-keeping in literary translation. Riassunto - Il presente studio si propone di ricavare e analizzare tendenze caratterizzanti il mercato della letteratura tradotta dall’inglese all’italiano in Italia e dall’italiano all’inglese in Regno Unito e negli Stati Uniti. I dati sono stati estratti da Index Translationum, una database bibliografico internazionale di traduzioni gestito dall’UNESCO, e sono stati inseriti in tre database Microsoft Acess, uno per ciascun Paese. I database sono stati analizzati secondo criteri specifici al fine di ricavare trend relativi alla quantità di letteratura tradotta nei tre Paesi, le maggiori case editrici che si interessano di letteratura tradotta, i generi e gli autori più tradotti. Questo studio intende fornire una base essenzialmente descrittiva ma necessaria per studi futuri sui processi socio-culturali e sulle dinamiche di mercato che influenzano le scelte degli editori, determinando così forme di controllo sul mercato della traduzione letteraria.

  7. Por una política de defensa común latinoamericana: la propuesta venezolana

    Directory of Open Access Journals (Sweden)

    Adriana Suzart de Pádua

    2010-09-01

    Full Text Available La búsqueda de la integración entre los países de América Latina pasa por un momento sui generis, en el que se trascienden los aspectos económicos y se confiere alguna relevancia a las discusiones sobre la elaboración de políticas de defensa para la región. En este escenario con diferentes percepciones sobre lo que constituyen amenazas, la región vive el surgimiento de dos propuestas de integración militar, una liderada por Brasil en el ámbito de la Unasur y otra liderada por Venezuela, en el ámbito del Alba. Este artículo presenta las bases de la propuesta venezolana para una integración en el área de defensa, específicamente, de las Fuerzas Armadas y hace un análisis de su viabilidad. AbstractThe search for integration among Latin American countries is at a unique point, with economic matters transcended in importance by discussion of the creation of defense policies for the region. In this scenario, with different perceptions regarding what constitutes a threat, two proposals for military integration exist, one lead by Brazil within the context of the Union of South American Nations (Unasur and the other lead by Venezuela in the context of the Bolivarian Alliance of the Peoples of Our America (Alba. This article presents the foundation of the Venezuelan proposal for the integration of an area of defense, specifically, by the Armed Forces, and provides an analysis of the proposal’s viability.

  8. Studio del comportamento di Acanthamoeba. polyphaga in presenza di Legionella pneumophila e di altri batteri ad habitat acquatico

    Directory of Open Access Journals (Sweden)

    M. Bondi

    2003-05-01

    Full Text Available Le amebe a vita libera sono state oggetto di diversi studi negli ultimi anni, non solo per le loro potenzialità patogene nei confronti dell’uomo, ma anche per l’importante ruolo che svolgono in natura, dove agiscono come predatori in grado di controllare le popolazioni batteriche. Alcuni degli organismi fagocitati però possono evitare la lisi fagosomiale e mantenere la loro condizione vitale a livello intracellulare, divenendo endosimbionti. Le amebe fungono così da riserva per questi batteri, proteggendoli da difficili condizioni extracellulari e provvedendo ad un ambiente consono alla loro replicazione. Tale tipo di interazione è particolarmente studiata in Legionella pneumophila, dal momento che l’ampia diffusione di questo germe, nonché la sua virulenza, pare siano fortemente influenzate dalla capacità di parassitare protozoi appartenenti ai generi Acanthamoeba, Naegleria e Balamuthia. Al fine di ottenere maggiori informazioni sui fattori favorenti o inibenti lo sviluppo di questi protozoi, è stato studiato il comportamento di un ceppo di Acanthamoeba polyphaga coltivato, in solido e in liquido, in associazione con L. pneumophila ed altri batteri ad habitat acquatico (Pseudomonas, Aeromonas, Achromobacter, Burkholderia. Su tappeti di cellule batteriche allestiti in Non Nutrient Agar (NNA, A.polyphaga si è mostrata in grado di moltiplicarsi utilizzando come nutrimento tutti i ceppi testati, nonostante alcuni, come Burkholderia cepacia SSV6 e Achromobacter xylox SS28, risultino più idonei al suo sviluppo. In piastre a pozzetti addizionate di acqua condottata autoclavata, il protozoo ha mostrato una buona capacità di sopravvivenza, non risultando inoltre influenzato dalla presenza di legionella o dei batteri acquatici testati. Dal momento che, fra i batteri descritti come capaci di vita intra-amebica, sono inclusi patogeni quali Chlamydia, Legionella, Listeria e Rickettsiae, risulta necessario riconsiderare la rilevanza clinica

  9. Some introductory notes on the problem of nuclear energy by controlled fusion reactions

    International Nuclear Information System (INIS)

    Pedretti, E.

    1988-01-01

    Written for scientists and technologist interested in, but unfamiliar with nuclear energy by controlled fusion reactions, this ''sui generis'' review paper attempts to provide the reader, as shortly as possible, with a general idea of the main issues at stake in nuclear fusion research. With the purpose of keeping this paper within a reasonable length, the various subjects are only outlined in their essence, basic features, underlying principles, etc., without entering into details, which are left to the quoted literature. Due to the particular readership of this journal, vacuum problems and/or aspects of fusion research anyhow related with vacuum science and technology are evidentiated. After reviewing fusion reactions' cross sections, fusion by accelerators and muon catalyzed fusion are described, followed by mention of Lawson's criteria and of plasma confinement features. Then, inertial confinement fusion is dealt with, also including one example of laser system (Nova), one of accelerator facility (PBFA-II) and some guesses on the classified Centurion-Halite program. Magnetic confinement fusion research is also reviewed, in particulary reporting one example of linear machine (MFTF-B), two examples of toroidal machines other than Tokamak (ATF and Eta-Beta-II) and various examples of Tokamaks, including PBX and PBX-M; TFTR, JET, JT-60, T-15 and Tore-Supra (large machines); Alcator A, FT, Alcator C/MTX, Alcator C-Mod and T-14 (compact high field machines). Tokamaks under design for ignition experiments (Ignitor, CIT, Ignitex and NET) are also illustrated. Thermal conversion of fusion power and direct generation of electricity are mentioned; conceptual design of fusion power plants are considered and illustrated by four examples (STARFIRE, WILDCAT, MARS and CASCADE). The D 3 He fuel cycle is discussed as an alternative more acceptable than Deuterium-Tritium, and thw Candor proposal is reported. After recalling past experience of the fission power development, some

  10. CONSIDERATIONS REGARDING THE SPECIFIC ELEMENTS OF THE REPURCHASE AGREEMENT (REPO

    Directory of Open Access Journals (Sweden)

    Bujorel Florea

    2014-11-01

    Full Text Available The current article focuses on the specific elements of the repurchase agreement, as they are regulated in the new Civil Code (Law no. 287/2009. In the beginning the author makes a general characterization of this type of contract, from the perspective of the specific elements regarding the contracting parties, the object of the contract and the moment of fulfilling certain obligations assumed by the parties. Then, the study defines the notions of “repo” and “reverse repo” and differentiates the repurchase agreement (repo from other similar contracts, configuring thus more clearly the analyzed convention. A specific element of the contract is represented by its legal nature of sui-generis contract, which the author explains by the fact that in the doctrine there is no unanimous opinion concerning this aspect. At the same time, the specificity of the repo is highlighted by presenting its main effects: the double transfer of property, the transmission of the accessory rights, the original buyer’s obligation to exercise his option, and the original seller’s obligations to make available for the original buyer the funds necessary for exercising the right of option and for making the payment. Last but not least, the specificity of this type of contract is revealed through reflecting the differences between the liquidation, prorogation and renewal of the debated convention. The study presents the viewpoints expressed in the literature, as well as the author’s opinions as regards the controversial legal problems in the studied field.

  11. Efforts of the British Empire to Build a State in Iraq After the First World War

    Directory of Open Access Journals (Sweden)

    İsmail Şahin

    2014-12-01

    Full Text Available This study searches for the policies carried out by the British Empire in Mesopotamia where the British invaded during the First World War. However, it will be convenient to know the political, social and economic background of Iraq or Mesopotamia as a general outline to understand the period clearly. Therefore, we have primarily tried to explain the overview of pre-invasion in Iraq. The traditional social structure of the Iraqi population and their relationship with the administration and each other have been questioned in the context of national identity. Then the developments in Iraq after the invasion have been discussed.The strategies which the Britain used to rule Iraq and the public’s reaction to this strategy have been investigated. In this context, the facts and the events such as the Mandate, 1920 Arab Revolt, the Cairo Conference of 1921 and the Kingdom of Iraq have been focused. It has been endeavored to per-form an analysis to understand the present problems in Iraq by discussing the cause and effect relations of the facts and the events. The research has been prepared by benefiting from a rich bibliography based on the scientific works of the experts for Iraq and the Middle East. As a result, this research has revealed the difficulties in ruling Iraq both internally and externally due to its sui generis political, social and economic conditions and found out that the mistakes made in the nation building process have caused the instability occured at the present time in the country.

  12. Croatian Pilgrimages to Loreto from the 5000 Documents of the "Croatian Maritime Regesta" in the 18th Century - vol. I

    Directory of Open Access Journals (Sweden)

    Zrinka Podhraški Čizmek

    2017-12-01

    Full Text Available This paper presents a small part of the pilgrimages undertaken by Croats during the 18th century across the Adriatic Sea to Loreto. The pilgrimages are described as a sui generis phenomenon of all human and religious societies. The history of the Catholic pilgrimage is analyzed through the perspective of common roots with Judaism and Islam, and the subsequent differentiation from other Christian confessions. Relations of the Croats with overseas territories since the 14th century are described, as well as their settlement in the hinterland of Ancona: Recanati and Loreto. The story of the Shrine of the Our Lady of Loreto is presented, the ties with the Croats settled there, and the foundation of a special seminary for Croats in 1580: the Illyrian College. During the review of the 4,890 documentary sources of volume I of the Croatian Maritime Regesta, a smaller segment of sources was found from the State Archive of Venice on the subject of pilgrimages. These sources list 44 pilgrimages from various locations on the Croatian coast. The types of ships they traveled on, the origin of the passengers, their number, organization in groups, the time of pilgrimage and the time of the year in which they occurred are analyzed. The most numerous pilgrims are from Lošinj (Lussino, followed by Cres (Cherso, Dugi Otok (Isola Lunga, Rovinj (Rovigno, Labin (Albona, Korčula (Curzola, Zadar (Zara and Vis (Lissa.This work is a contribution to the study of the links between the two sides of the Adriatic in the 18th century, which requires further publication and analysis of documentary sources.

  13. O MOSAICO NARRATIVO INTERSEMIÓTICO DE WATCHMEN: UM MODELO DE CONSTRUÇÃO POTENCIAL DE TEXTOS CRIATIVOS

    Directory of Open Access Journals (Sweden)

    Luiz Marcelo Brandão Carneiro

    2008-01-01

    Full Text Available Este trabajo es el fruto del desarrollo de una tesis de magíster, que se celebró en la Pontificia Universidad Católica de São Paulo, en el Programa de Comunicación y Semiótica. El objeto de estudio es el cómic Watchmen, escrito por Alan Moore e ilustrado por Dave Gibbons. Watchmen es una narrativa de estructura sui generis, de tela con parámetros específicos de su idioma, sino también (y que marca su diferencia, colocando el cuero rico artística y cultural de referencia, que establecerá los procedimientos importantes por sí solo y en concepto general de un balance de caracteres, de los capítulos y de la historia en su conjunto. El trabajo está dirigido por las teorías del lenguaje y por las teorías de la semiótica, más específicamente por las teorías de Mijail Bakhtin - en lo que respecta a la polifonía y el diálogo - y por los conceptos de intertextualidad e intersemioticidad, cuya principal referencia son los estudios de Julia Kristeva y Julio Plaza, respectivamente. En este artículo, la aplicación de intersemioticidad sobre la labor de estudio pone la intersemiotica como el núcleo de la construcción de la narrativa, apuntando caminos que se utilizarán en la concepción e en la confección de los textos verbales o verbo-visuales.

  14. Elementary Particle Spectroscopy in Regular Solid Rewrite

    International Nuclear Information System (INIS)

    Trell, Erik

    2008-01-01

    The Nilpotent Universal Computer Rewrite System (NUCRS) has operationalized the radical ontological dilemma of Nothing at All versus Anything at All down to the ground recursive syntax and principal mathematical realisation of this categorical dichotomy as such and so governing all its sui generis modalities, leading to fulfilment of their individual terms and compass when the respective choice sequence operations are brought to closure. Focussing on the general grammar, NUCRS by pure logic and its algebraic notations hence bootstraps Quantum Mechanics, aware that it ''is the likely keystone of a fundamental computational foundation'' also for e.g. physics, molecular biology and neuroscience. The present work deals with classical geometry where morphology is the modality, and ventures that the ancient regular solids are its specific rewrite system, in effect extensively anticipating the detailed elementary particle spectroscopy, and further on to essential structures at large both over the inorganic and organic realms. The geodetic antipode to Nothing is extension, with natural eigenvector the endless straight line which when deployed according to the NUCRS as well as Plotelemeian topographic prescriptions forms a real three-dimensional eigenspace with cubical eigenelements where observed quark-skewed quantum-chromodynamical particle events self-generate as an Aristotelean phase transition between the straight and round extremes of absolute endlessness under the symmetry- and gauge-preserving, canonical coset decomposition SO(3)xO(5) of Lie algebra SU(3). The cubical eigen-space and eigen-elements are the parental state and frame, and the other solids are a range of transition matrix elements and portions adapting to the spherical root vector symmetries and so reproducibly reproducing the elementary particle spectroscopy, including a modular, truncated octahedron nano-composition of the Electron which piecemeal enter into molecular structures or compressed to each

  15. Ciclo biológico, comportamiento y censo del picudo del camu camu, Conotrachelus dubiae O'Brien 1995 (Coleoptera: Curculionidae en Pucallpa, Perú Biological cycle, behavior and census of camu camu weevil, Conotrachelus dubiae O'Brien 1995 (Coleoptera: Curculionidae, in Pucallpa, Peru

    Directory of Open Access Journals (Sweden)

    Diana Perez

    2008-01-01

    Full Text Available El picudo, Conotrachelus dubiae O'Brien 1995, es una de las plagas mas importantes del camu camu Myrciaria dubia H.B.K. Mc Vaugh en la Amazonía Peruana. El objetivo del presente estudio fue determinar el ciclo biológico de este insecto bajo condiciones de laboratorio y describir su comportamiento y fluctuación en condiciones de campo en Pucallpa, Ucayali, Perú. El porcentaje de eclosión de larvas fue de 87%, la duración del periodo de incubación de los huevos fue de 5,5±0,9 (4 a 7 días, del estado larval en el fruto 22,2±1,9 (20 a 25 días y en el suelo (fase pre-pupa, 54,4±5,5 (46 a 67 días, del periodo pupal 11,8±0,9 (9 a 13 días y la longevidad del adulto fue de 51,8±18,9 (9 a 75 días. Los adultos se alimentaron de frutos de diferentes diámetros y estados de maduración y de botones florales, ramas tiernas y flores. No se registró la presencia de adultos de C. dubiae en frutos secos, ni en la base del tallo, sino en ritidomas. La mayor actividad de alimentación y de reproducción de los adultos fue entre 18:30 a 22:00 h. Los adultos fueron observados en el cultivo durante todo el año, encontrándose con mayor frecuencia en los meses de enero a marzo en pisos bajos inundables y entre octubre a diciembre en tierra firme no inundable, coincidiendo con la fase de floración y fructificación de la planta.Camu camu weevil Conotrachelus dubiae O'Brien, 1995 is a one of the main pests of camu camu (Myrciaria dubia H.B.K. Mc Vaugh in Peruvian Amazonia. The aim of this study was to determine the biological cycle of this insect under laboratory conditions, to describe its behavior and population numbers under field conditions in Pucallpa, Ucayali, Peru. The percentage of hatching was 87%; the egg incubation period was 5.5±0.9 (4 to 7 days; the length of the larval stage inside the fruit was 22.2±1.9 (20 to 25 days, and the length larval stage (pre-pupa underground was 54.4±5.5 (46 to 67 days. The length of pupal period was 11.8±0.9 (9 to 13 days, and the longevity of adults was 51.8±18.9 (9 to 75 days. Adults feed on fruits of different diameters and state of ripeness. Weevils also feed on buds, fresh branches and flowers. Adults of C. dubiae were only found in the rhizome, not in dry fruits or on the stem base.. The highest feeding and reproductive activity of adults was between 18:30 to 22:00 h. Adults were observed during the whole year, with the highest frequency between January and March in low seasonally flooded soils and between October to December in upland unflooded soils, coinciding with flowering and fruiting plant phenology.

  16. Educazione alla legalità, alla responsabilità sociale e all’inclusione. Una ricerca sui temi dell’antimafia sociale

    Directory of Open Access Journals (Sweden)

    Laura Corazza

    2016-11-01

    Full Text Available This research is part of an agreement with the regional government. The study focused on education for democratic lawfulness and civic responsibility by observing educational courses run by Libera on property confiscated from organized crime. The first outcome consisted of setting out pedagogical paradigms for education on democratic lawfulness and anti-mafia education. In order to do this, volunteers from schools were observed over a number of weeks while they lived in properties confiscated from mafia and worked on the land, which had been entrusted to co-operatives as part of an anti-mafia social training Program. Three Italian regions were involved: Piemonte, Emilia Romagna and Puglia. The second outcome was the production of multimedia materials for teaching, and specific courses, which were held in local secondary schools.

  17. Museologia e tecnologia 3D - Applicazioni sui reperti del Museo Archeologico di Aidone in mostra al The J. Paul Getty Museum ( Malibu, aprile 2012-gennaio 2013

    Directory of Open Access Journals (Sweden)

    Erika Piccillo

    2014-06-01

    Full Text Available Il progetto rigurda la scansione e la riproduzione, mediante tecnologia laser scanner 3D, di 19 reperti archeologici (datati dal IV al III secolo a.C. che, dal Museo Archeologico Regionale di Aidone (Sicilia, Italia sono stati dati in prestito, dopo il rientro della “Dea di Morgantina”, al The J.Paul Getty Museum di Malibu (California, USA, dove da aprile 2012 a gennaio 2013 si è tenuta la mostra temporanea “The Sanctuaries of Demeter andPersephoneat Morgantina”.Il caso studiato e documentato riguarda le scansioni, mediante prototipo David Lasercanner®dei suddetti reperti. Il lavoro è stato svolto al fine di documentare con la massima precisione e riprodurre  la copia virtuale fedele dei reperti dati in prestito al The J.Paul Getty Museum di Malibu, per poter verificare, al rientro degli stessi,il loro  stato dopo gli interventi di restauro eseguiti negli Stati Uniti oeventuali danneggiamenti dovuti al loro trasporto. Tali scansioni potrebbero in seguito anche essere utilizzate per aspetti volti alla promozione, alla fruizione (mostra virtualein situ o itinerante e la valorizzazione dei reperti presi in esame.  

  18. Climate change convention : reallocation of carbon dioxide emissions; Convenzione sui cambiamenti climatici: ipotesi per una assegnazione di quote di emissioni di anidride carbonica

    Energy Technology Data Exchange (ETDEWEB)

    Turchetti, P; Venanzi, M [ENEA, Centro Ricerche Casaccia, Rome (Italy). Dip. Ambiente

    1995-07-01

    In 1992, during the United Nation`s Conference on Environment and Development, held in Rio de Janeiro, the Framework Convention on Climate Change was signed. The ultimate objective of this Convention is the control and the reduction of greenhouse gas releases from human activities, to achieve stabilization of greenhouse gas concentrations in the atmosphere. The purpose of this research is to provide a reallocation of carbon dioxide important greenhouse gas, among nine countries representing the different geopolitical worldwide situations. Demographic, socioeconomic and environmental criteria have been taken into account. Some of these issues have been proposed in the international negotiating context. In order to apportion emissions the Analytic Hierarchy Process (AHP) has been used. The AHP is a multi objective decision-making technique employing a method of pairwise comparisons to rank order alternatives of a problem formulated in a hierarchic structure by computing the largest eigenvalue of the pairwise comparisons matrix. Moreover, the AHP approach enables one to deal with both quantitative and qualitative factors in a logical fashion. The findings summarize the different indicators considered representing the proposals of the parties involved in the international debate.

  19. MRI role in morphological and functional assessment of the levator ani muscle: Use in patients affected by stress urinary incontinence (SUI) before and after pelvic floor rehabilitation

    International Nuclear Information System (INIS)

    Del Vescovo, Riccardo; Piccolo, Claudia Lucia; Vecchia, Nicoletta Della; Giurazza, Francesco; Cazzato, Roberto Luigi; Grasso, Rosario Francesco; Zobel, Bruno Beomonte

    2014-01-01

    Objective: Pelvic floor dysfunctions affect a very high proportion of female population. Magnetic resonance imaging is the only technique able to provide a multiplanar overview of pelvic organs and muscles without the use of ionizing radiation. The aim of our prospective study is to objectively evaluate the effectiveness of perineal re-education applying MR technique. Materials and methods: 22 patients affected by stress urinary incontinence were enrolled in our prospective study. They underwent urogynaecological, urodynamic examinations, and a questionnaire about symptoms (ICIQ-UI) to investigate the degree of their interference with daily activities. Then they underwent a morphological and dynamic MR exam. Results: The pre-perineal rehabilitation MR examinations showed an asymmetry of the levator ani muscle in 87% of patients; the remaining 13% showed a muscular bilateral volume reduction. In the group with unilateral defect, the muscle total volume had values between 15 and 21 cm 3 . Its overall volume was 34.2% smaller on the defective side (9.28 ± 0.26 cm 3 ) compared to the normal side (12.64 ± 12.31 cm 3 , P < 0.001). In patients with a bilateral impairment, the muscle was replaced by fibro-fatty tissue, without a significant asymmetry between the two sides. The post-perineal rehabilitation MR tests showed three different degrees of response to therapy, with a “complete response” found in 67% of patients and no response in 13%. Conclusions: MR is an useful tool in the management of patients affected by stress urinary incontinence with indication for perineal rehabilitation. Its objective data allow to distinguish different types of response to therapy and, consequently, different outcomes in terms of additional treatments

  20. Occurrence of Cryptosporidium suis and Cryptosporidium scrofarum on commercial swine farms in the Czech Republic and its associations with age and husbandry practices

    Czech Academy of Sciences Publication Activity Database

    Němejc, K.; Sak, Bohumil; Květoňová, Dana; Kernerová, N.; Rost, M.; Cama, V. A.; Kváč, Martin

    2013-01-01

    Roč. 112, č. 3 (2013), s. 1143-1154 ISSN 0932-0113 R&D Projects: GA MŠk(CZ) LH11061 Grant - others:GAJU(CZ) 022/2010/Z Institutional support: RVO:60077344 Keywords : pig genotype II * post-weaned pig * molecular characterization * Sus scrofa * fecal transmission * Apicomplexa * risk factors * prevalence Subject RIV: EB - Genetics ; Molecular Biology Impact factor: 2.327, year: 2013 http://link.springer.com/article/10.1007%2Fs00436-012-3244-8