WorldWideScience

Sample records for cellular types accion

  1. Accions

    CERN Document Server

    Choi, Kang-Sin; Ramos-Sanchez, Saul; Vaudrevange, Patrick K S

    2009-01-01

    Axion fields provide the most elegant solution to the strong CP problem. In string compactifications it is difficult to obtain an axion whose decay constant is consistent with current cosmological bounds. We examine this question in theories with accidental U(1) symmetries that appear as low energy remnants of discrete symmetries. We refer to the axion-like particles from the spontaneous breakdown of these symmetries as accions. In such systems, the accion decay constant depends on the vacuum configuration and can be lowered to fit the bounds. Furthermore, we find that such accions with consistent decay constant can be embedded in special vacua of Z_6-II orbifold models with realistic features.

  2. Accions

    Science.gov (United States)

    Choi, Kang-Sin; Nilles, Hans Peter; Ramos-Sánchez, Saúl; Vaudrevange, Patrick K. S.

    2009-05-01

    Axion fields provide the most elegant solution to the strong CP problem. In string compactifications it is difficult to obtain an axion whose decay constant is consistent with current cosmological bounds. We examine this question in theories with accidental U(1) symmetries that appear as low energy remnants of discrete symmetries. We refer to the axion-like particles from the spontaneous breakdown of these symmetries as accions. In such systems, the accion decay constant depends on the vacuum configuration and can be lowered to fit the bounds. Furthermore, we find that such accions with consistent decay constant can be embedded in special vacua of Z6-II orbifold models with realistic features.

  3. Accions

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Kang-Sin [Department of Physics, Kyoto University, Kyoto 606-8502 (Japan); Nilles, Hans Peter [Bethe Center for Theoretical Physics and Physikalisches Institut der Universitaet Bonn, Nussallee 12, 53115 Bonn (Germany); Ramos-Sanchez, Saul [Deutsches Elektronen-Synchrotron DESY, Hamburg (Germany)], E-mail: ramos@mail.desy.de; Vaudrevange, Patrick K.S. [Arnold Sommerfeld Center for Theoretical Physics, Ludwig-Maximilians-Universitaet Muenchen, 80333 Muenchen (Germany)

    2009-05-18

    Axion fields provide the most elegant solution to the strong CP problem. In string compactifications it is difficult to obtain an axion whose decay constant is consistent with current cosmological bounds. We examine this question in theories with accidental U(1) symmetries that appear as low energy remnants of discrete symmetries. We refer to the axion-like particles from the spontaneous breakdown of these symmetries as accions. In such systems, the accion decay constant depends on the vacuum configuration and can be lowered to fit the bounds. Furthermore, we find that such accions with consistent decay constant can be embedded in special vacua of Z{sub 6}-II orbifold models with realistic features.

  4. Accions

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Kang-Sin [Kyoto Univ. (Japan). Dept. of Physics; Nilles, Hans Peter [Bonn Univ. (Germany). Bethe Center for Theoretical Physics and Physikalisches Inst.; Ramos-Sanchez, Saul [Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Vaudrevange, Patrick K.S. [Muenchen Univ. (Germany). Arnold Sommerfeld Center for Theoretical Physics

    2009-02-15

    Axion fields provide the most elegant solution to the strong CP problem. In string compactifications it is difficult to obtain an axion whose decay constant is consistent with current cosmological bounds. We examine this question in theories with accidental U(1) symmetries that appear as low energy remnants of discrete symmetries. We refer to the axion-like particles from the spontaneous breakdown of these symmetries as accions. In such systems, the accion decay constant depends on the vacuum configuration and can be lowered to fit the bounds. Furthermore, we find that such accions with consistent decay constant can be embedded in special vacua of Z{sub 6}-II orbifold models with realistic features. (orig.)

  5. Conjunct action of gamma radiation and casiopeinas in two cellular types; Accion conjunta de radiacion gamma y casiopeinas en dos tipos celulares

    Energy Technology Data Exchange (ETDEWEB)

    Ramos V, N.I

    2006-07-01

    In Mexico the cancer occupies the second place in the causes of death and although already there are diverse useful treatments to attack this suffering, the search of new pharmaceuticals that present positive effect in the treatment of the cancer there is taken to the development of a group of substances called Casiopeinas. Its are coordination compounds constituted by a central atom of copper (Cu II) joined to organic ligands, with general condensed formula [Cu (NN)(ON)]NO{sub 3}, or [Cu(NN)(OO)]NO{sub 3}. Its present different cytotoxicity levels, as well as in vitro antineoplastic activity in diverse tissues derived of tumors. Even its ignore the mechanisms of it action, but one of them, it could be by generation of reactive species of oxygen (ERO), mediated by the metallic center that contain. Thinking of this possibility was carried out in vitro experiments, exposing lymphocytes or HeLa cells, to ionizing radiation, at two casiopeinas (Casiopeina llgly and the Casiopeina Ill-e-a) or the combination of both agents, in order to determine if the antineoplastic capacity of both types of treatments increase in conjunct action. To determine it its were carried out cytotoxicity tests by means of the differential dyeing of fluorescein diacetate and etidio bromide, in these the cells alive fluorescein in green color while the dead ones only the nucleus of red tinged. The genotoxicity was evaluated by means of the comet essay. The results hurtled that as much the casiopeinas as the radiation by separate don't present difference among both cellular types, probably due to the used method that only quantifies the damages at the moment and not to long term. And according to those treatments on the whole only to the concentrations and the doses more high it favors the additive effect due to an overproduction of free radicals that not can be neutralized. According to the genotoxicity, only proven for the radiation indicates that the HeLa cells presents a major number of

  6. Sociedad por Acciones Simplificada

    OpenAIRE

    Carlos Andrés Arcila Salazar

    2009-01-01

    En Colombia, aspectos como la responsabilidad limitada; supresión de la llamada doctrina de ultra vires; duración ilimitada; eliminación de la regla, una acción, un voto; remedios al abuso de los derechos; posibilidad de eliminar prohibiciones a los administradores, libertad de establecer restricciones para la transferencia de acciones y la simplificación de los procedimientos de fusión; son inviables bajo nuestra legislación. Sin embargo, la Ley 1258 de 2008, ha incorporado al derecho societ...

  7. Sociedad por Acciones Simplificada

    Directory of Open Access Journals (Sweden)

    Carlos Andrés Arcila Salazar

    2009-06-01

    Full Text Available En Colombia, aspectos como la responsabilidad limitada; supresión de la llamada doctrina de ultra vires; duración ilimitada; eliminación de la regla, una acción, un voto; remedios al abuso de los derechos; posibilidad de eliminar prohibiciones a los administradores, libertad de establecer restricciones para la transferencia de acciones y la simplificación de los procedimientos de fusión; son inviables bajo nuestra legislación. Sin embargo, la Ley 1258 de 2008, ha incorporado al derecho societario colombiano un nuevo tipo societario híbrido, llamado “Sociedad por Acciones Simplificada”. Esta forma societaria híbrida se basa en un principio básico: garantizar amplia libertad a los empresarios en la estructuración de sus relaciones a través de disposiciones contractuales. La S.A.S. provee a los empresarios significativa flexibilidad contractual, a la vez que preserva beneficios como la limitación de la responsabilidad.

  8. Sociedad por Acciones Simplificada

    Directory of Open Access Journals (Sweden)

    Carlos Andrés Arcila Salazar

    2009-07-01

    Full Text Available En Colombia, aspectos como la responsabilidad limitada; supresión de la llamada doctrina de ultra vires; duración ilimitada; eliminación de la regla, una acción, un voto; remedios al abuso de los derechos; posibilidad de eliminar prohibiciones a los administradores, libertad de establecer restricciones para la transferencia de acciones y la simplificación de los procedimientos de fusión; son inviables bajo nuestra legislación. Sin embargo, la Ley 1258 de 2008, ha incorporado al derecho societario colombiano un nuevo tipo societario híbrido, llamado “Sociedad por Acciones Simplificada”. Esta forma societaria híbrida se basa en un principio básico: garantizar amplia libertad a los empresarios en la estructuración de sus relaciones a través de disposiciones contractuales. La S.A.S. provee a los empresarios significativa flexibilidad contractual, a la vez que preserva beneficios como la limitación de la responsabilidad.

  9. Mujeres en accion: design and baseline data.

    Science.gov (United States)

    Keller, Colleen; Fleury, Julie; Perez, Adriana; Belyea, Michael; Castro, Felipe G

    2011-10-01

    The majority of programs designed to promote physical activity in older Hispanic women includes few innovative theory-based interventions that address cultural relevant strategies. The purpose of this report is to describe the design and baseline data for Mujeres en Accion, a physical activity intervention to increase regular physical activity, and cardiovascular health outcomes among older Hispanic women. Mujeres en Accion [Women in Action for Health], a 12 month randomized controlled trial to evaluate the effectiveness of a social support physical activity intervention in midlife and older Hispanic women. This study tests an innovative intervention, Mujeres en Accion, and includes the use of a theory-driven approach to intervention, explores social support as a theoretical mediating variable, use of a Promotora model and a Community Advisory group to incorporate cultural and social approaches and resources, and use of objective measures of physical activity in Hispanic women. PMID:21298400

  10. Acciones sin voto en la empresa familiar

    OpenAIRE

    Balagué López, Laura

    2013-01-01

    El artículo 98 del Real Decreto Legislativo 1/2010, de la Ley de Sociedades de Capital (TRLSC), brinda a las sociedades anónimas la posibilidad de crecer a partir de un nuevo mecanismo de financiación, las acciones sin voto. Esta clase de acciones, fueron introducidas de la mano del sistema anglosajón en pleno s. XIX, con la vocación de aportar financiamiento externo a las sociedades mercantiles, sin afectar al control de las mismas. Dicho mecanismo, de escaso uso en general, no ha pasado des...

  11. Typing of murine cell-surface antigens by cellular radioimmunoassay

    International Nuclear Information System (INIS)

    A cellular radioimmunoassay utilizing 125I-labelled Protein A was used for detecting antigen-antibody complexes on gultaraldehyde fixed cells attached to microtiter plates. This method is rapid, sensitive and specific for revealing H-2 private and public specificities as well as Ia and Lyt antigens. As plates may be kept for months, several reactivities can be tested in one step on a large panel rendering a regular supply of animals unnecessary. (Auth.)

  12. A mathematical model of amphibian skin epithelium with two types of transporting cellular units

    DEFF Research Database (Denmark)

    Larsen, Erik Hviid; Rasmussen, B E

    1985-01-01

    A computer model of ion transport across amphibian skin epithelium containing two types of cellular units, their relative number and sizes, and a paracellular pathway has been developed. The two cellular units are, a large Na+ transporting compartment representing the major epithelium from stratum...

  13. Underlay of low-rate machine-type D2D links on downlink cellular links

    DEFF Research Database (Denmark)

    Pratas, Nuno K.; Popovski, Petar

    2014-01-01

    Wireless cellular networks feature two emerging technological trends: direct Device-to-Device (D2D) communications and Machine-Type Communications (MTC). MTC devices (MTDs) pose new challenges to the cellular network, such as low transmission power and massive access that can lead to overload...... of the radio interface. In this paper we explore the opportunity opened by D2D links for supporting Low-rate Low-power MTDs that are connected to a nearby device, such as an on-body MTD connected to a mobile phone that acts as a relay towards the Base Station (BS). The low-rate requirement for this D2D...... connection allows underlay operation with Successive Interference Cancellation (SIC) during the cellular downlink transmissions. We consider different ways to use SIC and investigate the trade-off between, on one hand, the achieved rate for the downlink cellular users and, on the other hand, the outage...

  14. Cellular fibronectin response to supervised moderate aerobic training in patients with type 2 diabetes.

    Science.gov (United States)

    Alghadir, Ahmad H; Gabr, Sami A; Al-Eisa, Einas

    2016-04-01

    [Purpose] Physical activity is one of the most pivotal targets for the prevention and management of vascular complications, especially endothelial dysfunctions. Cellular fibronectin is an endothelium-derived protein involved in subendothelial matrix assembly. Its plasma levels reflect matrix alterations and vessel wall destruction in patients with type II diabetes. This study investigated the influence of 12 weeks of supervised aerobic training on cellular fibronectin and its relationship with insulin resistance and body weight in type II diabetic subjects. [Subjects and Methods] This study included 50 men with type II diabetes who had a mean age of 48.8 ± 14.6 years and were randomly divided into two groups: an aerobic exercise group (12 weeks, three 50 minutes sessions per week) and control group. To examine changes in cellular fibronectin, glycosylated hemoglobin, insulin resistance, fasting insulin, fasting blood sugar, and lipid profile, 5 ml of blood was taken from the brachial vein of patients before and 48 hours after completion of the exercise period and after 12 hours of fasting at rest. Data analysis was performed using the SPSS-16 software with the independent and paired t-tests. [Results] A significant decrease was observed in body mass index and body fat percentage in the experimental group. Compared with the control group, the aerobic exercise group showed a significant decrease in cellular fibronectin, glycosylated hemoglobin, insulin resistance, fasting insulin, fasting blood sugar, and lipid profile after 12 weeks of aerobic exercise. The change in cellular fibronectin showed positive significant correlation with body mass index, diabetic biomarkers, and physical activity level. [Conclusion] The results showed that supervised aerobic exercise as a stimulus can change the levels of cellular fibronectin as matrix metalloproteinase protein a long with improvement of insulin sensitivity and glycosylated hemoglobin in order to prevent

  15. Hipnoticos corticales y basilares y acciones anticonvulsivantes

    Directory of Open Access Journals (Sweden)

    Carlos Gutiérrez-Noriega

    1943-06-01

    Full Text Available Se estudian las diferencias de los hipnóticos corticales y basilares desde el punto de vista de sus acciones anticonvulsivantes. Los resultados son los siguientes: 1. Los hipnóticos del grupo basilar (barbitúrico y uretano poseen un poder anticonvulsivante considerablemente mayor que los hipnóticos del grupo cortical (alcoholes, aldehidos, bromuros, éter, con excepción del sulfato de magnesia, clasificado en el grupo subcortical, que tiene muy poca acción anticonvulsivante. 2. Algunos hipnóticos del grupo cortical (cloralosa disminuyen notablemente a dosis narcótica el umbral para las convulsiones clónicas, producidas por el cardiazol, coramina y estricnina; pero aumentan el umbral para las convulsiones tónicas. Estos hipnóticos actúan paradójicamente, como anticonvulsivantes y como sinergistas de los convulsivantes. 3. El sinergismo de cardiazol y estricnina para producir convulsiones clónicas es mucho más notable durante la narcosis por cloralosa que en el animal no anestesiado. 4. Existen significativas diferencias cualitativas y cuantitativas entre las convulsiones del animal no anestesiado y las convulsiones del animal durante la narcosis por cloralosa. En este último caso no se produce la reacción tónica, las reacciones neurovegetativas son más débiles, pero la duración de las convulsiones clónicas es considerablemente mayer. 5. Las diferencias de actividad anticonvulsivante observadas entre los dos grupos de hipnóticos, no pueden ser tomadas como prueba de la clasificación topográfica (córtico-basilar. Al contrario, sugieren que algunos hipnóticos del grupo cortical deben su típica manera de actuar a una acción estimulante tanto sobre la corteza cerebral como sobre los centros subcorticales. Se discute la posibilidad de que el sistema internuncial sea el que principalmente se estimula. Así, mientras que en la narcosis de tipo barbitúrico disminuye la excitabilidad de todo el sistema nervioso de relaci

  16. Phase-Type Models of Channel-Holding Times in Cellular Communication Systems

    DEFF Research Database (Denmark)

    Christensen, Thomas Kaare; Nielsen, Bo Friis; Iversen, Villy Bæk

    2004-01-01

    In this paper, we derive the distribution of the channel-holding time when both cell-residence and call-holding times are phase-type distributed. Furthermore, the distribution of the number of handovers, the conditional channel-holding time distributions, and the channel-holding time when cell re...... residence times are correlated are derived. All distributions are of phase type, making them very general and flexible. The channel-holding times are of importance in performance evaluation and simulation of cellular mobile communication systems....

  17. Effects of type I collagen coating on titanium osseointegration: histomorphometric, cellular and molecular analyses

    International Nuclear Information System (INIS)

    The investigation of titanium (Ti) surface modifications aiming to increase implant osseointegration is one of the most active research areas in dental implantology. This study was carried out to evaluate the benefits of coating Ti with type I collagen on the osseointegration of dental implants. Acid etched Ti implants (AETi), either untreated or coated with type I collagen (ColTi), were placed in dog mandibles for three and eight weeks for histomorphometric, cellular and molecular evaluations of bone tissue response. While the histological aspects were essentially the same with both implants being surrounded by lamellar bone trabeculae, histomorphometric analysis showed more abundant bone formation in ColTi, mainly at three weeks. Cellular evaluation showed that cells harvested from bone fragments in close contact with ColTi display lower proliferative capacity and higher alkaline phosphatase activity, phenotypic features associated with more differentiated osteoblasts. Confirming these findings, molecular analyses showed that ColTi implants up-regulates the expression of a panel of genes well known as osteoblast markers. Our results present a set of evidences that coating AETi with collagen fastens the osseointegration by stimulating bone formation at the cellular and molecular levels, making this combination of morphological and biochemical modification a promising approach to treat Ti surfaces. (paper)

  18. Expression weighted cell type enrichments reveal genetic and cellular nature of major brain disorders

    Directory of Open Access Journals (Sweden)

    Nathan Gerald Skene

    2016-01-01

    Full Text Available The cell types that trigger the primary pathology in many brain diseases remain largely unknown. One route to understanding the primary pathological cell type for a particular disease is to identify the cells expressing susceptibility genes. Although this is straightforward for monogenic conditions where the causative mutation may alter expression of a cell type specific marker, methods are required for the common polygenic disorders. We developed the Expression Weighted Cell Type Enrichment (EWCE method that uses single cell transcriptomes to generate the probability distribution associated with a gene list having an average level of expression within a cell type. Following validation, we applied EWCE to human genetic data from cases of epilepsy, Schizophrenia, Autism, Intellectual Disability, Alzheimer’s disease, Multiple Sclerosis and anxiety disorders. Genetic susceptibility primarily affected microglia in Alzheimer’s and Multiple Sclerosis; was shared between interneurons and pyramidal neurons in Autism and Schizophrenia; while intellectual disabilities and epilepsy were attributable to a range of cell-types, with the strongest enrichment in interneurons. We hypothesised that the primary cell type pathology could trigger secondary changes in other cell types and these could be detected by applying EWCE to transcriptome data from diseased tissue. In Autism, Schizophrenia and Alzheimer’s disease we find evidence of pathological changes in all of the major brain cell types. These findings give novel insight into the cellular origins and progression in common brain disorders. The methods can be applied to any tissue and disorder and have applications in validating mouse models.

  19. Enterovirus 71 inhibits cellular type I interferon signaling by downregulating JAK1 protein expression.

    Science.gov (United States)

    Liu, Ying; Zhang, Zhe; Zhao, Xinghui; Yu, Rui; Zhang, Xiaopeng; Wu, Shipo; Liu, Ju; Chi, Xiangyang; Song, Xiaohong; Fu, Ling; Yu, Yingqun; Hou, Lihua; Chen, Wei

    2014-08-01

    Enterovirus 71 (EV71) infection can cause severe disease and lead to death in children. Recurring outbreaks of EV71 have been reported in several countries. Interferons (IFNs) have been used for decades to treat several types of viral infection, but have a limited ability to inhibit EV71 replication. Herein, we intend to investigate the mechanisms by which EV71 inhibits the cellular type I IFN response. In this study, MRC-5 (human embryonic lung fibroblast) or RD (human rhabdomyosarcoma) cells were infected with EV71, and then treated with or without IFN-α2b. Cells were harvested and analyzed by flow cytometry to determine the level of IFNAR1. Cell lysis were prepared to detect the levels of STAT1, STAT2, phosphorylated STAT1, phosphorylated STAT2, IFNAR1, JAK1, and TYK2 by Western blotting. The phosphorylation of STAT1 and STAT2 induced by IFN were inhibited without significant downregulation of IFNAR1 in EV71-infected cells. The EV71-induced suppression of STAT1 and STAT2 phosphorylation was not rescued by the protein tyrosine phosphatases inhibitor, and was independent of suppressor of cytokine signaling protein 1/3 levels. The phosphorylation of JAK1 and TYK2 were inhibited accompanied by EV71-induced downregulation of JAK1, which occurred at a post-transcriptional level and was proteasome independent. JAK1 expression did not decrease, and IFN-α-stimulated STAT1 and STAT2 phosphorylation were not blocked in HEK293T cells overexpressing the EV71 viral protein 2A or 3C. This study demonstrates that EV71 inhibits the cellular type I IFN antiviral pathway by downregulating JAK1, while the expression of IFNAR1 does not significantly alter in EV71-infected cells. Additionally, the EV71 viral proteins 2A and 3C do not act as antagonists of cellular type I IFN signaling. PMID:24905060

  20. A Quantum Cellular Automata architecture with nearest neighbor interactions using one quantum gate type

    CERN Document Server

    Ntalaperas, D

    2016-01-01

    We propose an architecture based on Quantum cellular Automata which allows the use of only one type of quantum gates per computational step in order to perform nearest neighbor interactions. The model is built in partial steps, each one of them analyzed using nearest neighbor interactions, starting with single qubit operations and continuing with two qubit ones. The effectiveness of the model is tested and valuated by developing a quantum circuit implementing the Quantum Fourier Transform. The important outcome of this validation was that the operations are performed in a local and controlled manner thus reducing the error rate of each computational step.

  1. LAS ACCIONES SOCIALES EN EMPRESAS CON RESPONSABILIDAD SOCIAL

    Directory of Open Access Journals (Sweden)

    Jorge Roberto, Volpentesta

    2012-12-01

    Full Text Available La investigación propone como objetivo general profundizar el conocimiento sobre las características de las acciones que las empresas realizan dentro de programas de Responsabilidad Social Empresaria con los grupos de interesados (stakeholders que se encuentran en la comunidad o sociedad, desde una perspectiva organizacional, pretendiendo esclarecer cuáles son las causas y/o circunstancias que determinan y explican el diseño de actividades tan disímiles que van desde las caracterizadas por un dirigismo centralizado propio de la aplicación de la lógica empresaria y en las cuales se trabaja desde un escritorio para los otros, hasta aquellas que desde sus inicios implican e involucran a los destinatarios de esas acciones generando verdaderas acciones con los otros.El interés por este conocimiento se basa en que cada vez más empresas están realizando acciones o programas de intervención en el campo social, pero que no siempre resultan efectivas considerando el impacto social provocado. Analizar los procedimientos utilizados, las circunstancias organizacionales, las causas estructurales y procesales y la lógica que impera en el diseño de esas acciones puede ayudar a justipreciar, a priori, su efectividad y potenciales resultados, facilitando el desarrollo de un posible modelo que oriente sobre los fundamentos necesarios para llevar a cabo intervenciones sociales exitosas.

  2. Cellular responses to HSV-1 infection are linked to specific types of alterations in the host transcriptome.

    Science.gov (United States)

    Hu, Benxia; Li, Xin; Huo, Yongxia; Yu, Yafen; Zhang, Qiuping; Chen, Guijun; Zhang, Yaping; Fraser, Nigel W; Wu, Dongdong; Zhou, Jumin

    2016-01-01

    Pathogen invasion triggers a number of cellular responses and alters the host transcriptome. Here we report that the type of changes to cellular transcriptome is related to the type of cellular functions affected by lytic infection of Herpes Simplex Virus type I in Human primary fibroblasts. Specifically, genes involved in stress responses and nuclear transport exhibited mostly changes in alternative polyadenylation (APA), cell cycle genes showed mostly alternative splicing (AS) changes, while genes in neurogenesis, rarely underwent these changes. Transcriptome wide, the infection resulted in 1,032 cases of AS, 161 incidences of APA, 1,827 events of isoform changes, and up regulation of 596 genes and down regulations of 61 genes compared to uninfected cells. Thus, these findings provided important and specific links between cellular responses to HSV-1 infection and the type of alterations to the host transcriptome, highlighting important roles of RNA processing in virus-host interactions. PMID:27354008

  3. Subculture of chondrocytes on a collagen type I-coated substrate with suppressed cellular dedifferentiation.

    Science.gov (United States)

    Kino-Oka, Masahiro; Yashiki, Shino; Ota, Yuka; Mushiaki, Yuko; Sugawara, Katsura; Yamamoto, Takeyuki; Takezawa, Toshiaki; Taya, Masahito

    2005-01-01

    To evaluate the degree of cellular dedifferentiation, subculture of chondrocytes was conducted on a surface coated with collagen type I at a density of 1.05 mg/cm(2). In the primary culture, most of the cells were round in shape on the collagen (CL) substrate, whereas fibroblastic and partially extended cells were dominant on the polystyrene plastic (PS) substrate. Stereoscopic observation revealed that the round-shaped cells on the CL substrate were hemispherical with nebulous and punctuated F-actin filaments, whereas the fibroblastic cells on the PS substrate were flattened with fully developed stress fibers. This suggested that cell polarization was suppressed during culture on the former substrate. Although serial passages of chondrocytes through subcultures on the CL and PS substrates caused a decrease in the number of round-shaped cells, the morphological change was appreciably suppressed on the CL substrate, as compared with that on the PS substrate. It was found that only round-shaped cells formed collagen type II, which supports the view that cellular dedifferentiation can be suppressed to some extent on the CL substrate. Three-dimensional cultures in collagen gel were performed with cells isolated freshly and passaged on the CL or PS substrate. Cell density at 21 days in the culture of cells passaged on the CL substrate was comparable to that in the culture of freshly isolated cells, in spite of a significant reduction in cell density observed in the culture of cells passaged on the PS substrate. In addition, histological analysis revealed that the expression of glycosaminoglycans and collagen type II was of significance in the collagen gel with cells passaged on the CL substrate, and likewise in the gel with freshly isolated cells. This indicated that the CL substrate could offer a monolayer culture system for expanding chondrocyte cells.

  4. Mujeres Unidas en Accion: A Popular Education Process.

    Science.gov (United States)

    Young, Eva; Padilla, Mariwilda

    1990-01-01

    Describes the development and structure of Mujeres Unidas en Accion, Inc., a nonprofit community-based agency in Dorchester, Massachusetts, that offers educational programs to low-income Latina women, and looks closely at one of its educational components, the Spanish program. (Author/JOW)

  5. An Experiment in Radiophonic Education: Accion Cultural Popular.

    Science.gov (United States)

    Masoner, Liliana Muhlmann de; And Others

    1982-01-01

    Describes the findings of a Florida State University evaluation of Colombia's Accion Cultural Popular (ACPO) program for nonformal adult education. ACPO promotes rural development and literacy through educational radio broadcasts which are linked to local monitors, textbooks, a weekly newspaper, and other support services. (AM)

  6. Accions sense vot en l'empresa familiar

    OpenAIRE

    Balagué López, Laura

    2013-01-01

    L'article 98 del Reial decret Legislatiu 1/2010, de la Llei de Societats de Capital (TRLSC), brinda a les societats anònimes la possibilitat de créixer a partir d'un nou mecanisme de finançament, les accions sense vot. Aquesta classe d'accions, van ser introduïdes de la mà del sistema anglosaxó en ple s. XIX, amb la vocació d'aportar finançament extern a les societats mercantils, sense afectar al control de les mateixes. Aquest mecanisme, d'escàs ús en general, no ha passat desapercebut per a...

  7. Bronchoalveolar Lavage Fluid Cellular and Haematological Changes in Different Types of Caprine Pneumonia.

    Science.gov (United States)

    Jarikre, A T; Emikpe, O B; Ohore, G O; Akinremi, A T; Akpavie, O S

    2016-01-01

    Goats in the tropics are often reared under the traditional extensive and semi-intensive management systems. These and other factors influence the pattern of pneumonia complex in goats. We investigated the bronchoalveolar lavage fluid (BALf) cellular changes and haematological response in different types of caprine pneumonia in Nigeria. Haematological indices and BALf cells were analysed from 300 goats randomly selected from 700 goats comprising different breed, age and body scores. The pneumonia status was well characterised using standard pathological tools. Data is summarized as Mean ± SEM and compared using non-parametric statistics at 5% significance. There was leukocytosis in the pneumonic animals. The overall lavage recovery rate was 55.5%. The differences in Haemoglobin concentration, and Lymphocyte-Neutrophil ratio were significant (peosinophil counts were significantly different (peosinophils observed in BAL were reliable and also correlated positively to the pathological findings. BAL should be considered a component of the diagnostic approach to caprine pneumonia complex, as it may accurately aid diagnosis and identification of the causal organisms. PMID:27574761

  8. Cellular angiofibroma: analysis of 25 cases emphasizing its relationship to spindle cell lipoma and mammary-type myofibroblastoma

    NARCIS (Netherlands)

    Flucke, U.E.; Krieken, J.H. van; Mentzel, T.

    2011-01-01

    Cellular angiofibroma represents a rare benign mesenchymal tumor, occurring mainly in the superficial soft tissue of the genital region. The involvement of 13q14 in some cases confirmed the morphological suggested link with spindle cell lipoma and mammary-type myofibroblastoma. We analyzed the clini

  9. Assessment of Membrane Fluidity Fluctuations during Cellular Development Reveals Time and Cell Type Specificity

    KAUST Repository

    Noutsi, Pakiza

    2016-06-30

    Cell membrane is made up of a complex structure of lipids and proteins that diffuse laterally giving rise to what we call membrane fluidity. During cellular development, such as differentiation cell membranes undergo dramatic fluidity changes induced by proteins such as ARC and Cofilin among others. In this study we used the generalized polarization (GP) property of fluorescent probe Laurdan using two-photon microscopy to determine membrane fluidity as a function of time and for various cell lines. A low GP value corresponds to a higher fluidity and a higher GP value is associated with a more rigid membrane. Four different cell lines were monitored such as hN2, NIH3T3, HEK293 and L6 cells. Membrane fluidity was measured at 12h, 72h and 92 h. Our results show significant changes in membrane fluidity among all cell types at different time points. GP values tend to increase significantly within 92 h in hN2 cells and 72 h in NIH3T3 cells and only at 92 h in HEK293 cells. L6 showed a marked decrease in membrane fluidity at 72 h and starts to increase at 92 h. As expected, NIH3T3 cells have more rigid membrane at earlier time points. On the other hand, neurons tend to have the highest membrane fluidity at early time points emphasizing its correlation with plasticity and the need for this malleability during differentiation. This study sheds light on the involvement of membrane fluidity during neuronal differentiation and development of other cell lines.

  10. Assessment of Membrane Fluidity Fluctuations during Cellular Development Reveals Time and Cell Type Specificity.

    Science.gov (United States)

    Noutsi, Pakiza; Gratton, Enrico; Chaieb, Sahraoui

    2016-01-01

    Cell membrane is made up of a complex structure of lipids and proteins that diffuse laterally giving rise to what we call membrane fluidity. During cellular development, such as differentiation cell membranes undergo dramatic fluidity changes induced by proteins such as ARC and Cofilin among others. In this study we used the generalized polarization (GP) property of fluorescent probe Laurdan using two-photon microscopy to determine membrane fluidity as a function of time and for various cell lines. A low GP value corresponds to a higher fluidity and a higher GP value is associated with a more rigid membrane. Four different cell lines were monitored such as hN2, NIH3T3, HEK293 and L6 cells. Membrane fluidity was measured at 12h, 72h and 92 h. Our results show significant changes in membrane fluidity among all cell types at different time points. GP values tend to increase significantly within 92 h in hN2 cells and 72 h in NIH3T3 cells and only at 92 h in HEK293 cells. L6 showed a marked decrease in membrane fluidity at 72 h and starts to increase at 92 h. As expected, NIH3T3 cells have more rigid membrane at earlier time points. On the other hand, neurons tend to have the highest membrane fluidity at early time points emphasizing its correlation with plasticity and the need for this malleability during differentiation. This study sheds light on the involvement of membrane fluidity during neuronal differentiation and development of other cell lines. PMID:27362860

  11. Conjunct action of gamma radiation and casiopeinas in two cellular types

    International Nuclear Information System (INIS)

    In Mexico the cancer occupies the second place in the causes of death and although already there are diverse useful treatments to attack this suffering, the search of new pharmaceuticals that present positive effect in the treatment of the cancer there is taken to the development of a group of substances called Casiopeinas. Its are coordination compounds constituted by a central atom of copper (Cu II) joined to organic ligands, with general condensed formula [Cu (NN)(ON)]NO3, or [Cu(NN)(OO)]NO3. Its present different cytotoxicity levels, as well as in vitro antineoplastic activity in diverse tissues derived of tumors. Even its ignore the mechanisms of it action, but one of them, it could be by generation of reactive species of oxygen (ERO), mediated by the metallic center that contain. Thinking of this possibility was carried out in vitro experiments, exposing lymphocytes or HeLa cells, to ionizing radiation, at two casiopeinas (Casiopeina llgly and the Casiopeina Ill-e-a) or the combination of both agents, in order to determine if the antineoplastic capacity of both types of treatments increase in conjunct action. To determine it its were carried out cytotoxicity tests by means of the differential dyeing of fluorescein diacetate and etidio bromide, in these the cells alive fluorescein in green color while the dead ones only the nucleus of red tinged. The genotoxicity was evaluated by means of the comet essay. The results hurtled that as much the casiopeinas as the radiation by separate don't present difference among both cellular types, probably due to the used method that only quantifies the damages at the moment and not to long term. And according to those treatments on the whole only to the concentrations and the doses more high it favors the additive effect due to an overproduction of free radicals that not can be neutralized. According to the genotoxicity, only proven for the radiation indicates that the HeLa cells presents a major number of cells with

  12. Acciones ciudadanas en defensa del Centro Jos?? Guerrero

    OpenAIRE

    Guill??n Marcos, Esperanza

    2010-01-01

    Este texto resume las acciones emprendidas por una plataforma, de iniciativa civil, en defensa del mantenimiento en la ciudad de Granada del Centro Jos?? Guerrero, cuya permanencia est?? en peligro por la decisi??n unilateral de la Diputaci??n de Granada de crear una ???Fundaci??n granadina de Arte Contempor??neo???, luego aprobada con el nombre de FNEO, y de estructura meramente pol??tica, que pretend??a privar de su autonom??a al centro. Liquidado este importante espacio de debate art??stic...

  13. Acciones biológicas y factores determinantes de las concentraciones del factor de crecimiento similar a la insulina tipo I (IGF-1 en el caballo (Biological actions and determinant factors of the circulating concentrations of insulin-like growth factor type 1, IGF-1, in the horse

    Directory of Open Access Journals (Sweden)

    Muñoz A

    2010-06-01

    Full Text Available ResumenEl eje somatotrópico es esencial para el desarrollo somático delorganismo, determinando el metabolismo celular. Aunque está constituido por diversas hormonas, los componentes esenciales son tres: la insulina, la hormona de crecimiento o GH y los factores de crecimiento similares a la insulina, tipos 1 y 2 (IGF-1 e IGF-2. La cuantificación de la GH es complicada debido, en primer lugar, a su liberación episódica hacia la circulación sistémica y en segundo lugar, a su corta vida media en sangre, unos 20 min. Uno de los lugares de actuación de la GH es el hígado, donde induce la síntesis de IGF-1 e IGF-2. La cuantificación de estos factores, por tanto, se considera un marcador fiable de la función hipofisaria y de laactividad del eje somatotrópico. En la presente revisión bibliográfica, en primer lugar, se describen las principales acciones biológicas del IGF-1. En segundo lugar, se presentan los factores principales que afectan significativamente a sus concentraciones circulantes. Entre estos factores, destacan el tamaño corporal, raza, edad, sexo, momento de la extracción de la muestra, ritmos circadianos, dieta, privación de comida, ejercicio y entrenamiento. El efecto de estos factores debe ser conocido antes de llevar a cabo una aplicación clínica e investigadora del IGF-1 en el caballo.SummaryThe somatotropic axis is pivotal for somatic development and cell metabolism. Although it is composed by several hormones, the most important components are three: insulin, growth hormone or GH and insulin-like growth factors type 1 and 2 (IGF- 1 and IGF-2. The measurement of GH is difficult and inaccurate, because two mainreasons: firstly, the GH hormone is released in an episodic way and secondly, its blood half-life is short, about 20 min. The GH hormone has significant actions on the liver, inducing the synthesis of IGF-1 and IGF-2. The measurement of these factors, therefore, is considered a reliable marker of the

  14. Development of an Insert Co-culture System of Two Cellular Types in the Absence of Cell-Cell Contact.

    Science.gov (United States)

    Renaud, Justine; Martinoli, Maria-Grazia

    2016-01-01

    The role of secreted soluble factors in the modification of cellular responses is a recurrent theme in the study of all tissues and systems. In an attempt to make straightforward the very complex relationships between the several cellular subtypes that compose multicellular organisms, in vitro techniques have been developed to help researchers acquire a detailed understanding of single cell populations. One of these techniques uses inserts with a permeable membrane allowing secreted soluble factors to diffuse. Thus, a population of cells grown in inserts can be co-cultured in a well or dish containing a different cell type for evaluating cellular changes following paracrine signaling in the absence of cell-cell contact. Such insert co-culture systems offer various advantages over other co-culture techniques, namely bidirectional signaling, conserved cell polarity and population-specific detection of cellular changes. In addition to being utilized in the field of inflammation, cancer, angiogenesis and differentiation, these co-culture systems are of prime importance in the study of the intricate relationships that exist between the different cellular subtypes present in the central nervous system, particularly in the context of neuroinflammation. This article offers general methodological guidelines in order to set up an experiment in order to evaluating cellular changes mediated by secreted soluble factors using an insert co-culture system. Moreover, a specific protocol to measure the neuroinflammatory effects of cytokines secreted by lipopolysaccharide-activated N9 microglia on neuronal PC12 cells will be detailed, offering a concrete understanding of insert co-culture methodology.

  15. Global Exponential Stability of a Unique Almost Periodic Solution for Neutral-Type Cellular Neural Networks with Distributed Delays

    Directory of Open Access Journals (Sweden)

    Wenquan Wu

    2014-01-01

    Full Text Available This paper is concerned with the problem of the existence, uniqueness, and global exponential stability for neutral-type cellular neural networks with distributed delays. Based on fixed point theory and Lyapunov functional, several sufficient conditions are established for the existence, uniqueness, and global exponential stability of almost periodic solution for the above system. Finally, a simple example is given to illustrate the feasibility and effectiveness of our main results.

  16. Global Exponential Stability of a Unique Almost Periodic Solution for Neutral-Type Cellular Neural Networks with Distributed Delays

    OpenAIRE

    Wenquan Wu

    2014-01-01

    This paper is concerned with the problem of the existence, uniqueness, and global exponential stability for neutral-type cellular neural networks with distributed delays. Based on fixed point theory and Lyapunov functional, several sufficient conditions are established for the existence, uniqueness, and global exponential stability of almost periodic solution for the above system. Finally, a simple example is given to illustrate the feasibility and effectiveness of our main results.

  17. Human papillomavirus type 16 E7 perturbs DREAM to promote cellular proliferation and mitotic gene expression

    OpenAIRE

    DeCaprio, James A.

    2013-01-01

    Study of the small DNA tumor viruses continues to provide valuable new insights into oncogenesis and fundamental biological processes. While much has already been revealed about how the human papillomaviruses (HPVs) can transform cells and contribute to cervical and oropharyngeal cancer, there clearly is much more to learn. In this issue of Oncogene, Pang et al. demonstrate that the high-risk HPV16 E7 oncogene can promote cellular proliferation by interacting with the DREAM (DP, RB-like, E2F ...

  18. Cellular localisation of type XIII collagen, and its induced expression in human neoplasias and corneal diseases

    OpenAIRE

    Väisänen, T. (Teemu)

    2005-01-01

    Abstract Type XIII collagen belongs to the group of transmembrane collagens. In this thesis the plasma membrane localisation and function of type XIII collagen have been studied using cell biological methods. Type XIII collagen was found to reside in focal adhesions. It appeared in these structures at a very early stage of their assembly and disappeared from them concurrently with focal adhesion proteins talin and vinculin. Insect cells expressing type XIII collagen showed an enhanced ...

  19. Comparison of cellular responses induced by low level light in different cell types

    Science.gov (United States)

    Huang, Ying-Ying; Chen, Aaron C.-H.; Sharma, Sulbha K.; Wu, Qiuhe; Hamblin, Michael R.

    2010-02-01

    Discoveries are rapidly being made in multiple laboratories that shed "light" on the fundamental molecular and cellular mechanisms underlying the use of low level light therapy (LLLT) in vitro, in animal models and in clinical practice. Increases in cellular levels of respiration, in cytochrome c oxidase activity, in ATP levels and in cyclic AMP have been found. Increased expression of reactive oxygen species and release of nitric oxide have also been shown. In order for these molecular changes to have a major effect on cell behavior, it is likely that various transcription factors will be activated, possibly via different signal transduction pathways. In this report we compare and contrast the effects of LLLT in vitro on murine embryonic fibroblasts, primary cortical neurons, cardiomyocytes and bone-marrow derived dendritic cells. We also examined two human cell lines, HeLa cancer cells and HaCaT keratinocytes. The effects of 810-nm near-infra-red light delivered at low and high fluences were addressed. Reactive oxygen species generation, transcription factor activation and ATP increases are reported. The data has led to the hypothesis that cells with a high level of mitochondrial activity (mitochondrial membrane potential) have a higher response to light than cells with low mitochondrial activity.

  20. A new flow-regulating cell type in the Demosponge Tethya wilhelma - functional cellular anatomy of a leuconoid canal system.

    Directory of Open Access Journals (Sweden)

    Jörg U Hammel

    Full Text Available Demosponges possess a leucon-type canal system which is characterized by a highly complex network of canal segments and choanocyte chambers. As sponges are sessile filter feeders, their aquiferous system plays an essential role in various fundamental physiological processes. Due to the morphological and architectural complexity of the canal system and the strong interdependence between flow conditions and anatomy, our understanding of fluid dynamics throughout leuconoid systems is patchy. This paper provides comprehensive morphometric data on the general architecture of the canal system, flow measurements and detailed cellular anatomical information to help fill in the gaps. We focus on the functional cellular anatomy of the aquiferous system and discuss all relevant cell types in the context of hydrodynamic and evolutionary constraints. Our analysis is based on the canal system of the tropical demosponge Tethya wilhelma, which we studied using scanning electron microscopy. We found a hitherto undescribed cell type, the reticuloapopylocyte, which is involved in flow regulation in the choanocyte chambers. It has a highly fenestrated, grid-like morphology and covers the apopylar opening. The minute opening of the reticuloapopylocyte occurs in an opened, intermediate and closed state. These states permit a gradual regulation of the total apopylar opening area. In this paper the three states are included in a theoretical study into flow conditions which aims to draw a link between functional cellular anatomy, the hydrodynamic situation and the regular body contractions seen in T. wilhelma. This provides a basis for new hypotheses regarding the function of bypass elements and the role of hydrostatic pressure in body contractions. Our study provides insights into the local and global flow conditions in the sponge canal system and thus enhances current understanding of related physiological processes.

  1. A new flow-regulating cell type in the Demosponge Tethya wilhelma - functional cellular anatomy of a leuconoid canal system.

    Science.gov (United States)

    Hammel, Jörg U; Nickel, Michael

    2014-01-01

    Demosponges possess a leucon-type canal system which is characterized by a highly complex network of canal segments and choanocyte chambers. As sponges are sessile filter feeders, their aquiferous system plays an essential role in various fundamental physiological processes. Due to the morphological and architectural complexity of the canal system and the strong interdependence between flow conditions and anatomy, our understanding of fluid dynamics throughout leuconoid systems is patchy. This paper provides comprehensive morphometric data on the general architecture of the canal system, flow measurements and detailed cellular anatomical information to help fill in the gaps. We focus on the functional cellular anatomy of the aquiferous system and discuss all relevant cell types in the context of hydrodynamic and evolutionary constraints. Our analysis is based on the canal system of the tropical demosponge Tethya wilhelma, which we studied using scanning electron microscopy. We found a hitherto undescribed cell type, the reticuloapopylocyte, which is involved in flow regulation in the choanocyte chambers. It has a highly fenestrated, grid-like morphology and covers the apopylar opening. The minute opening of the reticuloapopylocyte occurs in an opened, intermediate and closed state. These states permit a gradual regulation of the total apopylar opening area. In this paper the three states are included in a theoretical study into flow conditions which aims to draw a link between functional cellular anatomy, the hydrodynamic situation and the regular body contractions seen in T. wilhelma. This provides a basis for new hypotheses regarding the function of bypass elements and the role of hydrostatic pressure in body contractions. Our study provides insights into the local and global flow conditions in the sponge canal system and thus enhances current understanding of related physiological processes.

  2. Human papillomavirus type 16 E7 perturbs DREAM to promote cellular proliferation and mitotic gene expression.

    Science.gov (United States)

    DeCaprio, J A

    2014-07-31

    The study of the small DNA tumor viruses continues to provide valuable new insights into oncogenesis and fundamental biological processes. Although much has already been revealed about how the human papillomaviruses (HPVs) can transform cells and contribute to cervical and oropharyngeal cancer, there clearly is much more to learn. In this issue of Oncogene, Pang et al., doi:10.1038/onc.2013.426, demonstrate that the high-risk HPV16 E7 oncogene can promote cellular proliferation by interacting with the DREAM (DP, RB-like, E2F and MuvB) complex at two distinct phases of the cell cycle. Consistent with earlier work, HPV16 E7 can bind to the retinoblastoma tumor suppressor (RB) family member p130 (RBL2) protein and promote its proteasome-mediated destruction thereby disrupting the DREAM complex and can prevent exit from the cell cycle into quiescence. In addition, they demonstrate that HPV16 E7 can bind to MuvB core complex in association with BMYB and FOXM1 and activate gene expression during the G2 and M phase of the cell cycle. Thus, HPV16 E7 acts to prevent exit from the cell cycle entry and promotes mitotic proliferation and may account for the high levels of FOXM1 often observed in poor-risk cervical cancers. PMID:24166507

  3. Human papillomavirus type 16 E7 perturbs DREAM to promote cellular proliferation and mitotic gene expression

    Science.gov (United States)

    DeCaprio, James A.

    2014-01-01

    Study of the small DNA tumor viruses continues to provide valuable new insights into oncogenesis and fundamental biological processes. While much has already been revealed about how the human papillomaviruses (HPVs) can transform cells and contribute to cervical and oropharyngeal cancer, there clearly is much more to learn. In this issue of Oncogene, Pang et al. demonstrate that the high-risk HPV16 E7 oncogene can promote cellular proliferation by interacting with the DREAM (DP, RB-like, E2F and MuvB) complex at two distinct phases of the cell cycle (1). Consistent with earlier work, HPV16 E7 can bind to the retinoblastoma tumor suppressor (RB) family member p130 (RBL2) protein and promote its proteasome-mediated destruction thereby disrupting the DREAM complex and prevent exit from the cell cycle into quiescence. In addition, they demonstrate that HPV16 E7 can bind to MuvB core complex in association with BMYB and FOXM1 and activate gene expression during the G2 and M phase of the cell cycle. Thus, HPV16 E7 acts to prevent exit from the cell cycle entry and promotes mitotic proliferation and may account for the high levels of FOXM1 often observed in poor risk cervical cancers. PMID:24166507

  4. Geometric phase transition in the cellular network of the pancreatic islets may underlie the onset of type 1diabetes

    Science.gov (United States)

    Wang, Xujing

    Living systems are characterized by complexity in structure and emergent dynamic orders. In many aspects the onset of a chronic disease resembles phase transition in a dynamic system: quantitative changes accumulate largely unnoticed until a critical threshold is reached, which causes abrupt qualitative changes of the system. In this study we investigate this idea in a real example, the insulin-producing pancreatic islet β-cells and the onset of type 1 diabetes. Within each islet, the β-cells are electrically coupled to each other, and function as a network with synchronized actions. Using percolation theory we show how normal islet function is intrinsically linked to network connectivity, and the critical point where the islet cellular network loses site percolation, is consistent with laboratory and clinical observations of the threshold β-cell loss that causes islet functional failure. Numerical simulations confirm that the islet cellular network needs to be percolated for β-cells to synchronize. Furthermore, the interplay between site percolation and bond strength predicts the existence of a transient phase of islet functional recovery after disease onset and introduction of treatment, potentially explaining a long time mystery in the clinical study of type 1 diabetes: the honeymoon phenomenon. Based on these results, we hypothesized that the onset of T1D may be the result of a phase transition of the islet β-cell network. We further discuss the potential applications in identifying disease-driving factors, and the critical parameters that are predictive of disease onset.

  5. Discovery of novel, high potent, ABC type PTP1B inhibitors with TCPTP selectivity and cellular activity.

    Science.gov (United States)

    Liu, Peihong; Du, Yongli; Song, Lianhua; Shen, Jingkang; Li, Qunyi

    2016-08-01

    Protein tyrosine phosphatase 1B (PTP1B) as a key negative regulator of both insulin and leptin receptor pathways has been an attractive therapeutic target for the treatment of type 2 diabetes mellitus (T2DM) and obesity. With the goal of enhancing potency and selectivity of the PTP1B inhibitors, a series of methyl salicylate derivatives as ABC type PTP1B inhibitors (P1-P7) were discovered. More importantly, compound P6 exhibited high potent inhibitory activity (IC50 = 50 nM) for PTP1B with 15-fold selectivity over T-cell PTPase (TCPTP). Further studies on cellular activities revealed that compound P6 could enhance insulin-mediated insulin receptor β (IRβ) phosphorylation and insulin-stimulated glucose uptake. PMID:27123900

  6. Cellular signaling underlying atrial tachycardia remodeling of L-type calcium current

    NARCIS (Netherlands)

    Qi, Xiao Yan; Yeh, Yung-Hsin; Xiao, Ling; Burstein, Brett; Maguy, Ange; Chartier, Denis; Villeneuve, Louis R.; Brundel, Bianca J. J. M.; Dobrev, Dobromir; Nattel, Stanley

    2008-01-01

    Atrial tachycardia (AT) downregulates L-type Ca2+ current (I-CaL) and causes atrial fibrillation -promoting electric remodeling. This study assessed potential underlying signal transduction. Cultured adult canine atrial cardiomyocytes were paced at 0, 1, or 3 Hz (P0, P1, P3) for up to 24 hours. Cell

  7. Transglutaminase type 2-dependent selective recruitment of proteins into exosomes under stressful cellular conditions.

    Science.gov (United States)

    Diaz-Hidalgo, Laura; Altuntas, Sara; Rossin, Federica; D'Eletto, Manuela; Marsella, Claudia; Farrace, Maria Grazia; Falasca, Laura; Antonioli, Manuela; Fimia, Gian Maria; Piacentini, Mauro

    2016-08-01

    Numerous studies are revealing a role of exosomes in intercellular communication, and growing evidence indicates an important function for these vesicles in the progression and pathogenesis of cancer and neurodegenerative diseases. However, the biogenesis process of exosomes is still unclear. Tissue transglutaminase (TG2) is a multifunctional enzyme with different subcellular localizations. Particularly, under stressful conditions, the enzyme has been also detected in the extracellular matrix, but the mechanism(s) by which TG2 is released outside the cells requires further investigation. Therefore, the goal of the present study was to determine whether exosomes might be a vehicle for TG2 to reach the extracellular space, and whether TG2 could be involved in exosomes biogenesis. To address this issue, we isolated and characterized exosomes derived from cells either expressing or not TG2, under stressful conditions (i.e. proteasome impairment or expressing a mutated form of huntingtin (mHtt) containing 84 polyglutamine repeats). Our results show that TG2 is present in the exosomes only upon proteasome blockade, a condition in which TG2 interacts with TSG101 and ALIX, two key proteins involved in exosome biogenesis. Interestingly, we found that TG2 favours the assembly of a protein complex including mHtt, ALIX, TSG101 and BAG3, a co-chaperone involved in the clearance of mHtt. The formation of this complex is paralleled by the selective recruitment of mHtt and BAG3 in the exosomes derived from TG2 proficient cells only. Overall, our data indicate that TG2 is an important player in the biogenesis of exosomes controlling the selectivity of their cargo under stressful cellular conditions. In addition, these vesicles represent the way by which cells can release TG2 into the extracellular space under proteostasis impairment. PMID:27169926

  8. Preparation and cellular response of porous A-type carbonated hydroxyapatite nanoceramics

    Energy Technology Data Exchange (ETDEWEB)

    Li Bo, E-mail: Leewave@126.com [Institute of Biomaterials and Living Cell Imaging Technology, School of Metallurgy and Materials Engineering, Chongqing University of Science and Technology, Chongqing 401331 (China) and National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064 (China); Liao Xiaoling [Institute of Biomaterials and Living Cell Imaging Technology, School of Metallurgy and Materials Engineering, Chongqing University of Science and Technology, Chongqing 401331 (China); Zheng Li [National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064 (China); He Huawei [Department of Prosthodontics, Beijing Stomatological Hospital, Capital Medical University, Beijing, 100050 (China); Wang Hong [National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064 (China); Fan Hongsong, E-mail: hsfan68@hotmail.com [National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064 (China); Zhang Xingdong [National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064 (China)

    2012-05-01

    Microwave sintering using the activated carbon as embedding material was applied in preparation of porous A-type carbonated hydroxyapatite ceramics with nano(nCHA) and submicron (mCHA) structure. By examining the linear shrinkages and the compressive strengths of samples at different temperatures, a suitable microwave sintering temperature was achieved. The microwave sintering method was successfully used to prepare A-type CHA with nano or submicron structure, and the mechanism of the formation of A-type carbonate groups was discussed also. Compared with the samples prepared by the conventional sintering method (mHA), the nCHA bioceramics synthesized by the microwave sintering approach had smaller grain size and more uniform microstructure, and showed a compressive strength similar to the conventional samples. In vitro dissolution test proved that nCHA exhibits better degradation property in comparison to pure HA. Rat osteoblasts were cultured with nCHA, mCHA and mHA to evaluate their biocompatibility, and nCHA showed significant enhancement of cells in attachment, proliferation and differentiation. In conclusion, carbonate groups can be easily introduced to HA crystal structure using the activated carbon as embedding material, and microwave sintering is an effective and simple method in preparing A-type CHA with a nanostructure. Results from this in vitro biological study suggest that porous A-type carbonated hydroxyapatite nanoceramics may be a much better candidate for clinical use in terms of bioactivity. - Highlights: Black-Right-Pointing-Pointer We prepared porous A-type carbonated hydroxyapatite nanoceramics with microwave sintering. Black-Right-Pointing-Pointer We examined physico-chemical characterization and osteoblast response. Black-Right-Pointing-Pointer The nanoceramics have a comparable compressive strength to samples with conventional sintering method. Black-Right-Pointing-Pointer The nanoceramics enhance degradation property, osteoblast

  9. Cellular processing of the amyloidogenic cystatin C variant of hereditary cerebral hemorrhage with amyloidosis, Icelandic type

    DEFF Research Database (Denmark)

    Benedikz, Eirikur; Merz, G S; Schwenk, V;

    1999-01-01

    of an amyloidogenic mutation on the intracellular processing of its protein product. The protein, a mutant of the cysteine protease inhibitor cystatin C, is the amyloid precursor protein in Hereditary Cerebral Hemorrhage with Amyloidosis--Icelandic type (HCHWA-I). The amyloid fibers are composed of mutant cystatin C...... (L68Q) that lacks the first 10 amino acids. We have previously shown that processing of wild-type cystatin C entails formation of a transient intracellular dimer that dissociates prior to secretion, such that extracellular cystatin C is monomeric. We report here that the cystatin C mutation engenders...... several alterations in its intracellular trafficking. It forms a stable intracellular dimer that is partially retained in the endoplasmic reticulum and degraded. The bulk of mutant cystatin C that is secreted does not dissociate and is secreted as an inactive dimer. Thus, formation of the stable mutant...

  10. El enfermero en el contexto organizacional de las acciones programáticas de salud.

    OpenAIRE

    Pereira Passos, Joanir

    2006-01-01

    Se trata de un estudio descriptivo, con abordaje cualitativo, cuyos objetivos son: conocer el significado que tiene para los enfermeros la gerencia de las acciones programáticas de salud; identificar la importancia que los enfermeros dan a su papel en la gerencia de las acciones programáticas de salud; relacionar los conocimientos y las habilidades necesarias para la gerencia de las acciones programáticas de salud y discutir la preparación y el compromiso de los enfermeros...

  11. Natural and Semisynthetic Mammea-Type Isoprenlated Dihydroxycoumarins Uncouple Cellular Respiration

    Science.gov (United States)

    Du, Lin; Mahdi, Fakhri; Jekabsons, Mika B.; Nagle, Dale G.; Zhou, Yu-Dong

    2011-01-01

    In an effort to identify natural product-based molecular-targeted antitumor agents, mammea-type coumarins from the tropical/subtropical plant Mammea americana were found to inhibit the activation of HIF-1 (hypoxia-inducible factor-1) in human breast and prostate tumor cells. In addition to the recently reported mammea E/BB (15), bioassay-guided fractionation of the active extract yielded fourteen mammea-type coumarins including three new compounds mammea F/BB 1 (1), mammea F/BA (2), and C/AA (3). The absolute configuration of C-1′ in 1 was determined by the modified Mosher’s method on a methylated derivative. These coumarins were evaluated for their effects on mitochondrial respiration, HIF-1 signaling, and tumor cell proliferation/viability. Acetylation of 1 afforded a triacetoxylated product (A-2) that inhibited HIF-1 activation with increased potency in both T47D (IC50 0.83 μM for hypoxia-induced) and PC3 cells (IC50 0.94 μM for hypoxia-induced). Coumarins possessing a 6-prenyl-8-(3-methyl-oxobutyl)-substituent pattern exhibited enhanced HIF-1 inhibitory effects. The O-methylated derivatives were less active at inhibiting HIF-1 and suppressing cell proliferation/viability. Mechanistic studies indicate that these compounds act as anionic protonophores that potently uncouple mitochondrial electron transport and disrupt hypoxic signaling. PMID:21214226

  12. Translation efficiency determines differences in cellular infection among dengue virus type 2 strains

    International Nuclear Information System (INIS)

    We have investigated the molecular basis for differences in the ability of natural variants of dengue virus type 2 (DEN2) to replicate in primary human cells. The rates of virus binding, virus entry, input strand translation, and RNA stability of low-passage Thai and Nicaraguan and prototype DEN2 strains were compared. All strains exhibited equivalent binding, entry, and uncoating, and displayed comparable stability of positive strand viral RNA over time in primary cells. However, the low-passage Nicaraguan isolates were much less efficient in their ability to translate viral proteins. Sequence analysis of the full-length low-passage Nicaraguan and Thai viral genomes identified specific differences in the 3' untranslated region (3'UTR). Substitution of the different sequences into chimeric RNA reporter constructs demonstrated that the changes in the 3'UTR directly affected the efficiency of viral translation. Thus, differences in infectivity among closely related DEN2 strains correlate with efficiency of translation of input viral RNA

  13. The telomeric protein AKTIP interacts with A- and B-type lamins and is involved in regulation of cellular senescence

    Science.gov (United States)

    Burla, Romina; Carcuro, Mariateresa; Torre, Mattia La; Fratini, Federica; Crescenzi, Marco; D'Apice, Maria Rosaria; Spitalieri, Paola; Raffa, Grazia Daniela; Astrologo, Letizia; Lattanzi, Giovanna; Cundari, Enrico; Raimondo, Domenico; Biroccio, Annamaria; Gatti, Maurizio

    2016-01-01

    AKTIP is a shelterin-interacting protein required for replication of telomeric DNA. Here, we show that AKTIP biochemically interacts with A- and B-type lamins and affects lamin A, but not lamin C or B, expression. In interphase cells, AKTIP localizes at the nuclear rim and in discrete regions of the nucleoplasm just like lamins. Double immunostaining revealed that AKTIP partially co-localizes with lamin B1 and lamin A/C in interphase cells, and that proper AKTIP localization requires functional lamin A. In mitotic cells, AKTIP is enriched at the spindle poles and at the midbody of late telophase cells similar to lamin B1. AKTIP-depleted cells show senescence-associated markers and recapitulate several aspects of the progeroid phenotype. Collectively, our results indicate that AKTIP is a new player in lamin-related processes, including those that govern nuclear architecture, telomere homeostasis and cellular senescence. PMID:27512140

  14. RESPONSABILIDAD INTERNACIONAL DEL ESTADO COLOMBIANO POR ACCIONES DE GRUPOS PARAMILITARES

    Directory of Open Access Journals (Sweden)

    Nataly Vargas Ossa

    2008-12-01

    Full Text Available El Sistema Interamericano constituye un espacio que permite observar la situación de los Derechos Humanos en América Latina, los mecanismos de protección a que acuden las víctimas y la actitud de los Estados frente a la promoción, protección y defensa de los Derechos Humanos. Bajo esta premisa es que el proyecto “Alcance y Descripción de los Derechos Humanos en el Sistema Interamericano de Protección de los Derechos Humanos”, utilizando el método dogmático documental, pretendió recopilar aquellas providencias que permiten recoger el concepto y el alcance que La Corte Interamericana ha desarrollado entorno a algunos Derechos Humanos, es así como el presente escrito hace parte de los resultados de dicho proyecto, el cual recopila las sentencias proferidas por la Corte Interamericana en contra del Estado Colombiano, por acciones de grupos paramilitares.El estado Colombiano en reiteradas oportunidades ha sido declarado internacionalmente responsable por violaciones a Derechos Humanos, debido a la conformación y apoyo de grupos “paramilitares“. En el presente escrito se busca retomar aquellas providencias emanadas de la Corte Interamericana, en lo que tiene que ver con la descripción y alcance de derechos por los cuales el Estado Colombiano ha sido condenado. En es orden de ideas son cuatro providencias objeto análisis del presente escrito: El Caso de los 19 Comerciantes, el Caso de la Masacre de Mapiripán, el Caso de las Masacres de Pueblo Bello, y el Caso de las Masacres de Ituango.

  15. Cellular High-energy Cavitation Trauma - description of a novel in vitro trauma model in three different cell types.

    Directory of Open Access Journals (Sweden)

    Yuli eCao

    2016-02-01

    Full Text Available The mechanisms involved in traumatic brain injury (TBI have yet to be fully characterized. One mechanism that, especially in high energy trauma, could be of importance is cavitation. Cavitation can be described as a process of vaporization, bubble generation and bubble implosion as a result of a decrease and subsequent increase in pressure. Cavitation as an injury mechanism is difficult to visualize and model due to its short duration and limited spatial distribution. One strategy to analyze the cellular response of cavitation is to employ suitable in vitro models. The flyer plate is an in vitro high energy trauma model that includes cavitation as a trauma mechanism. A copper fragment is accelerated by means of a laser, hits the bottom of a cell culture well causing cavitation and shock waves inside the well and cell medium. We have found the flyer plate model to be efficient, reproducible and easy to control. In this study we have used the model to analyze the cellular response to microcavitation in SH-SY5Y neuroblastoma, Caco-2, and C6 glioma cell lines. Mitotic activity in neuroblastoma and glioma was investigated with BrdU staining, and cell numbers were calculated using automated time-lapse imaging. We found variations between cell types and between different zones surrounding the lesion with these methods. It was also shown that the injured cell cultures released S-100B in a dose dependent manner. Using gene expression microarray a number of gene families of potential interest were found to be strongly, but differently regulated in neuroblastoma and glioma at 24 hr post trauma. The data from the gene expression arrays may be used to identify new candidates for biomarkers in cavitation trauma. We conclude that our model is useful for studies of trauma in vitro and that it could be applied in future treatment studies.

  16. Cellular High-Energy Cavitation Trauma – Description of a Novel In Vitro Trauma Model in Three Different Cell Types

    Science.gov (United States)

    Cao, Yuli; Risling, Mårten; Malm, Elisabeth; Sondén, Anders; Bolling, Magnus Frödin; Sköld, Mattias K.

    2016-01-01

    The mechanisms involved in traumatic brain injury have yet to be fully characterized. One mechanism that, especially in high-energy trauma, could be of importance is cavitation. Cavitation can be described as a process of vaporization, bubble generation, and bubble implosion as a result of a decrease and subsequent increase in pressure. Cavitation as an injury mechanism is difficult to visualize and model due to its short duration and limited spatial distribution. One strategy to analyze the cellular response of cavitation is to employ suitable in vitro models. The flyer-plate model is an in vitro high-energy trauma model that includes cavitation as a trauma mechanism. A copper fragment is accelerated by means of a laser, hits the bottom of a cell culture well causing cavitation, and shock waves inside the well and cell medium. We have found the flyer-plate model to be efficient, reproducible, and easy to control. In this study, we have used the model to analyze the cellular response to microcavitation in SH-SY5Y neuroblastoma, Caco-2, and C6 glioma cell lines. Mitotic activity in neuroblastoma and glioma was investigated with BrdU staining, and cell numbers were calculated using automated time-lapse imaging. We found variations between cell types and between different zones surrounding the lesion with these methods. It was also shown that the injured cell cultures released S-100B in a dose-dependent manner. Using gene expression microarray, a number of gene families of potential interest were found to be strongly, but differently regulated in neuroblastoma and glioma at 24 h post trauma. The data from the gene expression arrays may be used to identify new candidates for biomarkers in cavitation trauma. We conclude that our model is useful for studies of trauma in vitro and that it could be applied in future treatment studies. PMID:26869990

  17. Cellular High-Energy Cavitation Trauma - Description of a Novel In Vitro Trauma Model in Three Different Cell Types.

    Science.gov (United States)

    Cao, Yuli; Risling, Mårten; Malm, Elisabeth; Sondén, Anders; Bolling, Magnus Frödin; Sköld, Mattias K

    2016-01-01

    The mechanisms involved in traumatic brain injury have yet to be fully characterized. One mechanism that, especially in high-energy trauma, could be of importance is cavitation. Cavitation can be described as a process of vaporization, bubble generation, and bubble implosion as a result of a decrease and subsequent increase in pressure. Cavitation as an injury mechanism is difficult to visualize and model due to its short duration and limited spatial distribution. One strategy to analyze the cellular response of cavitation is to employ suitable in vitro models. The flyer-plate model is an in vitro high-energy trauma model that includes cavitation as a trauma mechanism. A copper fragment is accelerated by means of a laser, hits the bottom of a cell culture well causing cavitation, and shock waves inside the well and cell medium. We have found the flyer-plate model to be efficient, reproducible, and easy to control. In this study, we have used the model to analyze the cellular response to microcavitation in SH-SY5Y neuroblastoma, Caco-2, and C6 glioma cell lines. Mitotic activity in neuroblastoma and glioma was investigated with BrdU staining, and cell numbers were calculated using automated time-lapse imaging. We found variations between cell types and between different zones surrounding the lesion with these methods. It was also shown that the injured cell cultures released S-100B in a dose-dependent manner. Using gene expression microarray, a number of gene families of potential interest were found to be strongly, but differently regulated in neuroblastoma and glioma at 24 h post trauma. The data from the gene expression arrays may be used to identify new candidates for biomarkers in cavitation trauma. We conclude that our model is useful for studies of trauma in vitro and that it could be applied in future treatment studies. PMID:26869990

  18. Precision automation of cell type classification and sub-cellular fluorescence quantification from laser scanning confocal images

    Directory of Open Access Journals (Sweden)

    Hardy Craig Hall

    2016-02-01

    Full Text Available While novel whole-plant phenotyping technologies have been successfully implemented into functional genomics and breeding programs, the potential of automated phenotyping with cellular resolution is largely unexploited. Laser scanning confocal microscopy has the potential to close this gap by providing spatially highly resolved images containing anatomic as well as chemical information on a subcellular basis. However, in the absence of automated methods, the assessment of the spatial patterns and abundance of fluorescent markers with subcellular resolution is still largely qualitative and time-consuming. Recent advances in image acquisition and analysis, coupled with improvements in microprocessor performance, have brought such automated methods within reach, so that information from thousands of cells per image for hundreds of images may be derived in an experimentally convenient time-frame. Here, we present a MATLAB-based analytical pipeline to 1 segment radial plant organs into individual cells, 2 classify cells into cell type categories based upon random forest classification, 3 divide each cell into sub-regions, and 4 quantify fluorescence intensity to a subcellular degree of precision for a separate fluorescence channel. In this research advance, we demonstrate the precision of this analytical process for the relatively complex tissues of Arabidopsis hypocotyls at various stages of development. High speed and robustness make our approach suitable for phenotyping of large collections of stem-like material and other tissue types.

  19. ACCIONES PARA LA AUTORREGULACIÓN DEL APRENDIZAJE EN ENTORNOS PERSONALES

    Directory of Open Access Journals (Sweden)

    Eduardo Chaves Barboza

    2016-01-01

    Full Text Available El objetivo del estudio es analizar las acciones que realizan los estudiantes de la población para la autorregulación del aprendizaje en entornos personales de aprendizaje. Se aplicó un cuestionario a una muestra de estudiantes de la Universidad de Granada (España que pertenecen a las titulaciones de Pedagogía, Educación Social y Psicopedagogía. Sobre los datos se efectúan pruebas no paramétricas de correlaciones y análisis de varianzas, así como estadística descriptiva e inferencial. Los resultados relacionan las acciones autorregulatorias que realizan los estudiantes y demuestran que estas acciones son homogéneas según titulación, sexo, edad y otras variables en estudio.

  20. Cell-type specific photoreceptors and light signaling pathways in the multicellular green alga volvox carteri and their potential role in cellular differentiation

    OpenAIRE

    Kianianmomeni, Arash

    2015-01-01

    The formation of multicellular organisms requires genetically predefined signaling pathways in various cell types. Besides differences in size, energy balance and life time, cell types should be enable to modulate appropriate developmental and adaptive responses in ever-changing surrounding environment. One of the most important environmental cues is light which regulates a variety of physiological and cellular processes. During evolution, diverse light-sensitive proteins, so-called photorece...

  1. Gastric Lgr5(+) stem cells are the cellular origin of invasive intestinal-type gastric cancer in mice.

    Science.gov (United States)

    Li, Xiu-Bin; Yang, Guan; Zhu, Liang; Tang, Yu-Ling; Zhang, Chong; Ju, Zhenyu; Yang, Xiao; Teng, Yan

    2016-07-01

    The cellular origin of gastric cancer remains elusive. Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5) is the first identified marker of gastric stem cells. However, the role of Lgr5(+) stem cells in driving malignant gastric cancer is not fully validated. Here, we deleted Smad4 and PTEN in murine gastric Lgr5(+) stem cells by the inducible Cre-LoxP system and marked mutant Lgr5(+) stem cells and their progeny with Cre-reporter Rosa26(tdTomato). Rapid onset and progression from microadenoma and macroscopic adenoma to invasive intestinal-type gastric cancer (IGC) were found in the gastric antrum with the loss of Smad4 and PTEN. In addition, invasive IGC developed at the murine gastro-forestomach junction, where a few Lgr5(+) stem cells reside. In contrast, Smad4 and PTEN deletions in differentiated cells, including antral parietal cells, pit cells and corpus Lgr5(+) chief cells, failed to initiate tumor growth. Furthermore, mutant Lgr5(+) cells were involved in IGC growth and progression. In the TCGA (The Cancer Genome Atlas) database, an increase in LGR5 expression was manifested in the human IGC that occurred at the gastric antrum and gastro-esophageal junction. In addition, the concurrent deletion of SMAD4 and PTEN, as well as their reduced expression and deregulated downstream pathways, were associated with human IGC. Thus, we demonstrated that gastric Lgr5(+) stem cells were cancer-initiating cells and might act as cancer-propagating cells to contribute to malignant progression. PMID:27091432

  2. Problemas en materia de documentación y transmisión de acciones

    OpenAIRE

    Alonso Espinosa, Francisco José

    1992-01-01

    Régimen de las acciones como partes de capital y como valores mobiliarios. Régimen de la emisión de las acciones desde la constitución de la sociedad anónima hasta la emisión de los títulos o la organización del registro contable de anotaciones en cuenta. Circulación de la acción como título. Resguardos provisionales, extractos de inscripición y títulos múltiples.

  3. Characterization of cellular convection of argon in top shield penetrations of pool type liquid metal fast reactors

    Energy Technology Data Exchange (ETDEWEB)

    Paliwal, Pranav; Parthasarathy, U. [Thermal Hydraulics Section, Nuclear and Safety Engineering Group, Indira Gandhi Centre for Atomic Research, Kalpakkam 603102 (India); Velusamy, K., E-mail: kvelu@igcar.gov.in [Thermal Hydraulics Section, Nuclear and Safety Engineering Group, Indira Gandhi Centre for Atomic Research, Kalpakkam 603102 (India); Sundararajan, T. [Department of Mechanical Engineering, IIT-Madras, Chennai 600036 (India); Chellapandi, P. [Thermal Hydraulics Section, Nuclear and Safety Engineering Group, Indira Gandhi Centre for Atomic Research, Kalpakkam 603102 (India)

    2012-09-15

    Highlights: Black-Right-Pointing-Pointer We present characterization of cellular convection by 3-D CFD analysis. Black-Right-Pointing-Pointer CFD model is validated against in-house experimental data. Black-Right-Pointing-Pointer We bring out effects of geometric and process parameters on cellular convection. Black-Right-Pointing-Pointer Correlations are developed to serve as guideline for design of FBR top shield. - Abstract: Cellular convection of argon gas that takes place in narrow annuli of component penetrations in FBR top shield has been investigated. The 3-D CFD simulations have been validated against in-house experimental data. The circumferential temperature difference in the shells, which is a result of cellular convection is given special attention. Cellular convection is seen to be highly sensitive to geometric and thermal conditions. For Rayleigh numbers (Ra) less than {approx}300, cellular convection effect is seen to be very weak. For Ra exceeding {approx}3 Multiplication-Sign 10{sup 8}, natural convection is vigorous with insignificant circumferential temperature variations. In the intermediate range of Rayleigh numbers, cellular convection assumes great significance. The maximum circumferential temperature difference in the component shell is seen to occur at Ra {approx} 3 Multiplication-Sign 10{sup 5}, which corresponds to a gap width of 50 mm. The number of convective cells increases with decrease in gap width as well as increase in forced cooling. The circumferential temperature difference is found to be directly proportional to annulus height, suggesting significant incentives in adopting shorter penetration heights. However, the circumferential temperature difference is found to be a weak function of annulus diameter. The number of cells is unfavorably less in an eccentric annulus compared to a concentric annulus. Appropriate correlations have been proposed to determine the circumferential temperature difference and number of convective cells

  4. Accion Cultural Popular. [Summary Working Documents on ACPO's Conceptual Framework and Activities].

    Science.gov (United States)

    Bernal, Hernando; And Others

    Three papers are included in this document designed to provide English language information about characteristics and activities of Accion Cultural Popular (ACPO) a private, nonprofit organization concerned with improving the quality of life of rural populations and promoting rural development through mass media education programs. "Pioneer…

  5. BECA (Bilingual Education Centro de Accion) Program Handbook for Student Teachers and Supervisory Personnel.

    Science.gov (United States)

    Reyes, Maria; And Others

    This manual is a reference guide for both student teachers and supervisory personnel involved with the Texas Woman's University Bilingual Education "Centro de Accion" (BECA) Program. The BECA program includes the following components in addition to the fulltime BECA undergraduate program: para-professional training program, graduate programs for…

  6. Pequenitos en Accion. Edgewood ISD Model Program for 3-Year-Olds Replication Manual.

    Science.gov (United States)

    Partnership for Hope, Inc., San Antonio, TX.

    This guide describes "Pequenitos en Accion" (Small Children in Action), the Edgewood (Texas) Independent School District (ISD) early childhood intervention program for Spanish-speaking 3-year-old preschool children. The program is an innovative early childhood education model involving educational programming, collaboration and integration with…

  7. Cellular function and pathological role of ATP13A2 and related P-type transport ATPases in Parkinson’s disease and other neurological disorders

    Directory of Open Access Journals (Sweden)

    Sarah evan Veen

    2014-05-01

    Full Text Available Mutations in ATP13A2 lead to Kufor-Rakeb syndrome, a parkinsonism with dementia. ATP13A2 belongs to the P-type transport ATPases, a large family of primary active transporters that exert vital cellular functions. However, the cellular function and transported substrate of ATP13A2 remain unknown. To discuss the role of ATP13A2 in neurodegeneration, we first provide a short description of the architecture and transport mechanism of P-type transport ATPases. Then, we briefly highlight key P-type ATPases involved in neuronal disorders such as the copper transporters ATP7A (Menkes disease, ATP7B (Wilson disease, the Na+/K+-ATPases ATP1A2 (familial hemiplegic migraine and ATP1A3 (rapid-onset dystonia parkinsonism. Finally, we review the recent literature of ATP13A2 and discuss ATP13A2’s putative cellular function in the light of what is known concerning the functions of other, better-studied P-type ATPases. We critically review the available data concerning the role of ATP13A2 in heavy metal transport and propose a possible alternative hypothesis that ATP13A2 might be a flippase. As a flippase, ATP13A2 may transport an organic molecule, such as a lipid or a peptide, from one membrane leaflet to the other. A flippase might control local lipid dynamics during vesicle formation and membrane fusion events.

  8. Periodic Solutions for Shunting Inhibitory Cellular Neural Networks of Neutral Type with Time-Varying Delays in the Leakage Term on Time Scales

    OpenAIRE

    Yongkun Li; Lei Wang; Yu Fei

    2014-01-01

    A class of shunting inhibitory cellular neural networks of neutral type with time-varying delays in the leakage term on time scales is proposed. Based on the exponential dichotomy of linear dynamic equations on time scales, fixed point theorems, and calculus on time scales we obtain some sufficient conditions for the existence and global exponential stability of periodic solutions for that class of neural networks. The results of this paper are completely new and complementary to the previous...

  9. Global Exponential Stability of Almost Periodic Solution for Neutral-Type Cohen-Grossberg Shunting Inhibitory Cellular Neural Networks with Distributed Delays and Impulses

    OpenAIRE

    Lijun Xu; Qi Jiang; Guodong Gu

    2016-01-01

    A kind of neutral-type Cohen-Grossberg shunting inhibitory cellular neural networks with distributed delays and impulses is considered. Firstly, by using the theory of impulsive differential equations and the contracting mapping principle, the existence and uniqueness of the almost periodic solution for the above system are obtained. Secondly, by constructing a suitable Lyapunov functional, the global exponential stability of the unique almost periodic solution is also investigated. The work ...

  10. Effective Inhibition of Cellular ROS Production by MXCXXC-Type Peptides: Potential Therapeutic Applications in Copper-Homeostasis Disorders.

    Science.gov (United States)

    Shoshan, Michal S; Tshuva, Edit Y

    2016-06-27

    Cyclic and acyclic peptides with sequences derived from metallochaperone binding sites, but differing at position 2, were analyzed for their inhibitory reactivity towards cellular ROS (reactive oxygen species) formation and catalytic activity towards oxidation with H2 O2 , in comparison with three commercial drugs clinically employed in chelation therapy for Wilson's disease. Acyclic peptides were more effective inhibitors than the cyclic ones, with one leading peptide with threonine at position 2 systematically showing the highest efficiency in reducing cellular ROS levels and in inhibiting Cu oxidation. This peptide was more effective than all commercial drugs in all aspects analyzed, and showed no toxicity towards human colon HT-29 cancer cells at concentrations 10-100 times higher than the IC50 of the commercial drugs, corroborating its high medicinal potential. PMID:27124086

  11. Cellular ATP Synthesis Mediated by Type III Sodium-dependent Phosphate Transporter Pit-1 Is Critical to Chondrogenesis*

    OpenAIRE

    Sugita, Atsushi; Kawai, Shinji; Hayashibara, Tetsuyuki; Amano, Atsuo; Ooshima, Takashi; Michigami, Toshimi; Yoshikawa, Hideki; Yoneda, Toshiyuki

    2010-01-01

    Disturbed endochondral ossification in X-linked hypophosphatemia indicates an involvement of Pi in chondrogenesis. We studied the role of the sodium-dependent Pi cotransporters (NPT), which are a widely recognized regulator of cellular Pi homeostasis, and the downstream events in chondrogenesis using Hyp mice, the murine homolog of human X-linked hypophosphatemia. Hyp mice showed reduced apoptosis and mineralization in hypertrophic cartilage. Hyp chondrocytes in culture displayed decreased ap...

  12. Evolution of Envelope Sequences of Human Immunodeficiency Virus Type 1 in Cellular Reservoirs in the Setting of Potent Antiviral Therapy

    OpenAIRE

    Gunthard, HF; Frost, SDW; Leigh Brown, AJ; Ignacio, CC; Kee, K; Perelson, AS; Spina, CA; Havlir, DV; Hezareh, M; Looney, DJ; Richman, DD; Wong, JK

    1999-01-01

    In human immunodeficiency virus (HIV)-infected patients treated with potent antiretroviral therapy, the persistence of latently infected cells may reflect the long decay half-life of this cellular reservoir or ongoing viral replication at low levels with continuous replenishment of the population or both. To address these possibilities, sequences encompassing the C2 and V3 domains of HIV-1 env were analyzed from virus present in baseline plasma and from viral isolates obtained after 2 years o...

  13. There is more than one way to turn a spherical cellular monolayer inside out: type B embryo inversion in Volvox globator

    Directory of Open Access Journals (Sweden)

    Höhn Stephanie

    2011-12-01

    Full Text Available Abstract Background Epithelial folding is a common morphogenetic process during the development of multicellular organisms. In metazoans, the biological and biomechanical processes that underlie such three-dimensional (3D developmental events are usually complex and difficult to investigate. Spheroidal green algae of the genus Volvox are uniquely suited as model systems for studying the basic principles of epithelial folding. Volvox embryos begin life inside out and then must turn their spherical cell monolayer outside in to achieve their adult configuration; this process is called 'inversion.' There are two fundamentally different sequences of inversion processes in Volvocaceae: type A and type B. Type A inversion is well studied, but not much is known about type B inversion. How does the embryo of a typical type B inverter, V. globator, turn itself inside out? Results In this study, we investigated the type B inversion of V. globator embryos and focused on the major movement patterns of the cellular monolayer, cell shape changes and changes in the localization of cytoplasmic bridges (CBs connecting the cells. Isolated intact, sectioned and fragmented embryos were analyzed throughout the inversion process using light microscopy, confocal laser scanning microscopy, scanning electron microscopy and transmission electron microscopy techniques. We generated 3D models of the identified cell shapes, including the localizations of CBs. We show how concerted cell-shape changes and concerted changes in the position of cells relative to the CB system cause cell layer movements and turn the spherical cell monolayer inside out. The type B inversion of V. globator is compared to the type A inversion in V. carteri. Conclusions Concerted, spatially and temporally coordinated changes in cellular shapes in conjunction with concerted migration of cells relative to the CB system are the causes of type B inversion in V. globator. Despite significant similarities

  14. Estrategias de Responsabilidad Social Corporativa: acciones lazo rosa en multinacionales de moda-belleza en España

    OpenAIRE

    Monserrat-Gauchi, Juan; Llopis-Ripoll, Carolina; Tur-Viñes, Victoria

    2014-01-01

    Esta investigación analiza las acciones de Responsabilidad Social Corporativa (RSC) -lazo rosa- llevadas a cabo por las empresas de moda-belleza multinacionales que operan en España. Los resultados sugieren que las acciones de RSC han sido planificadas de forma estratégica por la empresa, dentro de un programa de Responsabilidad Social, y que no forman parte del diseño de acciones de comunicación o marketing aisladas, pero tampoco de su filosofía empresarial. Las aportaciones son importantes ...

  15. Eficacia de las acciones preventivas del consumo de drogas en jóvenes

    OpenAIRE

    Agulló Calatayud, Víctor; Abellán Andrés, Carlos

    2011-01-01

    La Confederación Don Bosco viene desarrollando, desde el año 2005, en 80 poblaciones de toda España, la campaña «Entérate, si vas a salir», mediante una serie de acciones relacionadas con la prevención del consumo de drogas entre la población juvenil, así como diversas campañas de información y sensibilización sobre los riesgos y consecuencias del consumo de drogas y la diso- ciación entre éste y el concepto de diversión. Para el desarrollo de estas acciones venimos con- tando ...

  16. Accion solidaria. A Venezuelan NGO confronts the Herculean task of providing drug access.

    Science.gov (United States)

    Dougherty, S

    1997-05-01

    Venezuela does not currently recognize AIDS as a major public health problem, therefore, it does not fund AIDS services. It is estimated by the World Health Organization that Venezuela may have up to 250,000 HIV-infected citizens. Accion Solidaria (Action for Solidarity) is currently acting as a buyer's club, and as a source of medications from charitable groups in the United States such as the United Against AIDS International, for people living with HIV. Accion Solidaria is an entirely voluntary organization that receives monthly contributions from 41 people, just barely enough to purchase medications for 10 HIV-infected people. These efforts have been hindered by successive devaluations of Venezuelan currency. PMID:11364337

  17. Acciones públicas en la zona metropolitana de Monterrey, Nuevo León

    Directory of Open Access Journals (Sweden)

    Freddy Mariñez Navarro

    2007-01-01

    Full Text Available Existe una relación entre el capital social y las redes de políticas, como puntos fundamentales para la definición de las políticas públicas, con participación de actores. En este caso se analizan datos empíricos del capital social en los nueve municipios de la zona metropolitana de Monterrey, Nuevo León.También, se identifican los contextos de las redes de políticas tanto en las acciones públicas de dichos municipios, como en el proyecto de Ciudad Internacional del Conocimiento. El artículo concluye que un enfoque de redes de políticas provee una herramienta útil para el análisis del proceso de las políticas públicas, y a la vez refuerza la eficacia y legitimidad de las acciones públicas.

  18. Las acciones punitivas físicas: una perspectiva intercultural

    Directory of Open Access Journals (Sweden)

    María del Mar BADIA MARTÍN

    2009-11-01

    Full Text Available RESUMEN: En este artículo se enmarca el término de castigo corporal diferenciándolo del de abuso físico, a la vez que se realiza un análisis sobre la perspectiva de diferentes países con relación a la aplicación de dicha forma de castigo. La investigación y la teoría analizada, muestran cómo ciertas sociedades todavía reclaman el uso del castigo corporal como una forma más para frenar los comportamientos molestos. Esto nos puede llevar a pensar que no se debe abandonar la tesis sobre el hecho de que esta estrategia es rechazada en la actualidad por todos los sectores de la sociedad, sino que debemos ser sensibles a la idea de que ciertos profesionales y familias lo consideran un instrumento más para la corrección de conductas consideradas como no correctas.ABSTRACT: In this article the term corporal punishment is differentiated from that of physical abuse, and at the same time an analysis is made of the perspective of different countries in relation to the application of this type of punishment. The research and theory analysed show how certain societies still call for the use of corporal punishment as one more way to stop troublesome behaviour. This can lead us to think that the thesis that this strategy is currently rejected by all sectors of society should not be abandoned, but we should be aware that certain professionals and families still consider it one more instrument for correcting behaviour considered as incorrect.

  19. Cell type-dependent induction of DNA damage by 1800 MHz radiofrequency electromagnetic fields does not result in significant cellular dysfunctions.

    Directory of Open Access Journals (Sweden)

    Shanshan Xu

    Full Text Available BACKGROUND: Although IARC clarifies radiofrequency electromagnetic fields (RF-EMF as possible human carcinogen, the debate on its health impact continues due to the inconsistent results. Genotoxic effect has been considered as a golden standard to determine if an environmental factor is a carcinogen, but the currently available data for RF-EMF remain controversial. As an environmental stimulus, the effect of RF-EMF on cellular DNA may be subtle. Therefore, more sensitive method and systematic research strategy are warranted to evaluate its genotoxicity. OBJECTIVES: To determine whether RF-EMF does induce DNA damage and if the effect is cell-type dependent by adopting a more sensitive method γH2AX foci formation; and to investigate the biological consequences if RF-EMF does increase γH2AX foci formation. METHODS: Six different types of cells were intermittently exposed to GSM 1800 MHz RF-EMF at a specific absorption rate of 3.0 W/kg for 1 h or 24 h, then subjected to immunostaining with anti-γH2AX antibody. The biological consequences in γH2AX-elevated cell type were further explored with comet and TUNEL assays, flow cytometry, and cell growth assay. RESULTS: Exposure to RF-EMF for 24 h significantly induced γH2AX foci formation in Chinese hamster lung cells and Human skin fibroblasts (HSFs, but not the other cells. However, RF-EMF-elevated γH2AX foci formation in HSF cells did not result in detectable DNA fragmentation, sustainable cell cycle arrest, cell proliferation or viability change. RF-EMF exposure slightly but not significantly increased the cellular ROS level. CONCLUSIONS: RF-EMF induces DNA damage in a cell type-dependent manner, but the elevated γH2AX foci formation in HSF cells does not result in significant cellular dysfunctions.

  20. Accion dark matter in the post-inflationary Peccei-Quinn symmetry breaking scenario

    CERN Document Server

    Ringwald, Andreas

    2015-01-01

    We consider extensions of the Standard Model in which a spontaneously broken global chiral Peccei-Quinn (PQ) symmetry arises as an accidental symmetry of an exact $Z_N$ symmetry. For $N = 9$ or $10$, this symmetry can protect the accion - the Nambu-Goldstone boson arising from the spontaneous breaking of the accidental PQ symmetry - against semi-classical gravity effects, thus providing a proper solution of the strong CP problem, while it can at the same time provide for the small explicit symmetry breaking term needed to make models with domain wall number $N_{\\rm DW}>1$, such as the popular DFSZ model ($N_{\\rm DW}=6$), cosmologically viable even in the case where spontaneous PQ symmetry breaking occurred after inflation. We find that $N=10$ DFSZ accions with mass $m_A \\approx 3.5$-$4.2\\,\\mathrm{meV}$ can account for cold dark matter and simultaneously explain the hints for anomalous cooling of white dwarfs. The proposed helioscope IAXO - being sensitive to solar DFSZ accions with mass above a few meV - will...

  1. A New Flow-Regulating Cell Type in the Demosponge Tethya wilhelma – Functional Cellular Anatomy of a Leuconoid Canal System

    Science.gov (United States)

    Hammel, Jörg U.; Nickel, Michael

    2014-01-01

    Demosponges possess a leucon-type canal system which is characterized by a highly complex network of canal segments and choanocyte chambers. As sponges are sessile filter feeders, their aquiferous system plays an essential role in various fundamental physiological processes. Due to the morphological and architectural complexity of the canal system and the strong interdependence between flow conditions and anatomy, our understanding of fluid dynamics throughout leuconoid systems is patchy. This paper provides comprehensive morphometric data on the general architecture of the canal system, flow measurements and detailed cellular anatomical information to help fill in the gaps. We focus on the functional cellular anatomy of the aquiferous system and discuss all relevant cell types in the context of hydrodynamic and evolutionary constraints. Our analysis is based on the canal system of the tropical demosponge Tethya wilhelma, which we studied using scanning electron microscopy. We found a hitherto undescribed cell type, the reticuloapopylocyte, which is involved in flow regulation in the choanocyte chambers. It has a highly fenestrated, grid-like morphology and covers the apopylar opening. The minute opening of the reticuloapopylocyte occurs in an opened, intermediate and closed state. These states permit a gradual regulation of the total apopylar opening area. In this paper the three states are included in a theoretical study into flow conditions which aims to draw a link between functional cellular anatomy, the hydrodynamic situation and the regular body contractions seen in T. wilhelma. This provides a basis for new hypotheses regarding the function of bypass elements and the role of hydrostatic pressure in body contractions. Our study provides insights into the local and global flow conditions in the sponge canal system and thus enhances current understanding of related physiological processes. PMID:25409176

  2. Both viral E2 protein and the cellular factor PEBP2 regulate transcription via E2 consensus sites within the bovine papillomavirus type 4 long control region.

    OpenAIRE

    Jackson, M E; Campo, M. S.

    1995-01-01

    The bovine papillomavirus type 4 (BPV4) long control region (LCR) contains three consensus binding sites, E2(1), E2(2), and E2(3) (ACCN6GGT), for the viral E2 transcription factor and a fourth degenerate site, dE2 (ATCN6GGT), which lies 3 bp upstream of E2(3). The E2(2) site was found to bind the cellular transcription factor PEBP2, and mutations at this site reduced basal promoter activity by as much as 60%, indicating an important role for PEBP2 in LCR function. Mutation of the E2(3) or dE2...

  3. Global Exponential Stability of Almost Periodic Solution for Neutral-Type Cohen-Grossberg Shunting Inhibitory Cellular Neural Networks with Distributed Delays and Impulses

    Directory of Open Access Journals (Sweden)

    Lijun Xu

    2016-01-01

    Full Text Available A kind of neutral-type Cohen-Grossberg shunting inhibitory cellular neural networks with distributed delays and impulses is considered. Firstly, by using the theory of impulsive differential equations and the contracting mapping principle, the existence and uniqueness of the almost periodic solution for the above system are obtained. Secondly, by constructing a suitable Lyapunov functional, the global exponential stability of the unique almost periodic solution is also investigated. The work in this paper improves and extends some results in recent years. As an application, an example and numerical simulations are presented to demonstrate the feasibility and effectiveness of the main results.

  4. Global Exponential Stability of Almost Periodic Solution for Neutral-Type Cohen-Grossberg Shunting Inhibitory Cellular Neural Networks with Distributed Delays and Impulses

    Science.gov (United States)

    Xu, Lijun; Jiang, Qi; Gu, Guodong

    2016-01-01

    A kind of neutral-type Cohen-Grossberg shunting inhibitory cellular neural networks with distributed delays and impulses is considered. Firstly, by using the theory of impulsive differential equations and the contracting mapping principle, the existence and uniqueness of the almost periodic solution for the above system are obtained. Secondly, by constructing a suitable Lyapunov functional, the global exponential stability of the unique almost periodic solution is also investigated. The work in this paper improves and extends some results in recent years. As an application, an example and numerical simulations are presented to demonstrate the feasibility and effectiveness of the main results. PMID:27190502

  5. Periodic Solutions for Shunting Inhibitory Cellular Neural Networks of Neutral Type with Time-Varying Delays in the Leakage Term on Time Scales

    Directory of Open Access Journals (Sweden)

    Yongkun Li

    2014-01-01

    Full Text Available A class of shunting inhibitory cellular neural networks of neutral type with time-varying delays in the leakage term on time scales is proposed. Based on the exponential dichotomy of linear dynamic equations on time scales, fixed point theorems, and calculus on time scales we obtain some sufficient conditions for the existence and global exponential stability of periodic solutions for that class of neural networks. The results of this paper are completely new and complementary to the previously known results even if the time scale =ℝ or ℤ. Moreover, we present illustrative numerical examples to show the feasibility of our results.

  6. Impact of pnpR, a LysR-type regulator-encoding gene, on the cellular processes of Pseudomonas putida DLL-E4.

    Science.gov (United States)

    Chen, Qiongzhen; Tu, Hui; Huang, Fei; Wang, Yicheng; Dong, Weiliang; Wang, Wenhui; Li, Zhoukun; Wang, Fei; Cui, Zhongli

    2016-06-01

    LysR-type transcriptional regulators (LTTRs) regulate various cellular processes in bacteria. pnpR is an LTTR-encoding gene involved in the regulation of hydroquinone (HQ) degradation, and its effects on the cellular processes of Pseudomonas putida DLL-E4 were investigated at the physiological, biochemical and molecular levels. Reverse transcription polymerase chain reaction revealed that pnpR positively regulated its own expression and that of the pnpC1C2DECX1X2 operon; additionally, pnpR partially regulated the expression of pnpA when P. putida was grown on para-nitrophenol (PNP) or HQ. Strains DLL-E4 and DLL-ΔpnpR exhibited similar cellular morphologies and growth rates. Transcriptome analysis revealed that pnpR regulated the expression of genes in addition to those involved in PNP degradation. A total of 20 genes were upregulated and 19 genes were downregulated by at least 2-fold in strain DLL-ΔpnpR relative to strain DLL-E4. Bioinformatic analysis revealed putative PnpR-binding sites located in the upstream regions of genes involved in PNP degradation, carbon catabolite repression and other cellular processes. The utilization of L-aspartic acid, L-histidine, L-pyroglutamic acid, L-serine, γ-aminobutyric acid, D,L-lactic acid, D-saccharic acid, succinic acid and L-alaninamide was increased at least 1.3-fold in strain DLL-ΔpnpR as shown by BIOLOG assays, indicating that pnpR plays a potential negative regulation role in the utilization of carbon sources. PMID:27190157

  7. Immunohistochemical characterization of cell types expressing the cellular prion protein in the small intestine of cattle and mice.

    Science.gov (United States)

    Miyazawa, Kohtaro; Kanaya, Takashi; Tanaka, Sachi; Takakura, Ikuro; Watanabe, Kouichi; Ohwada, Shyuichi; Kitazawa, Haruki; Rose, Michael T; Sakaguchi, Suehiro; Katamine, Shigeru; Yamaguchi, Takahiro; Aso, Hisashi

    2007-03-01

    The gastrointestinal tract is thought to be the main site of entry for the pathological isoform of the prion protein (PrP(Sc)). Prion diseases are believed to result from a conformational change of the cellular prion protein (PrP(c)) to PrP(Sc). Therefore, PrP(c) expression is a prerequisite for the infection and spread of the disease to the central nervous system. However, the distribution of PrP(c) in the gut is still a matter of controversy. We therefore investigated the localization of PrP(c) in the bovine and murine small intestine. In cattle, most PrP(c) positive epithelial cells were detected in the duodenum, while a few positive cells were found in the jejunum. PrP(c) was expressed in serotonin producing cells. In bovine Peyer's patches, PrP(c) was distributed in extrafollicular areas, but not in the germinal centre of the jejunum and ileum. PrP(c) was expressed in myeloid lineage cells such as myeloid dendritic cells and macrophages. In mice, PrP(c) was expressed in some epithelial cells throughout the small intestine as well as in cells such as follicular dendritic cell in the germinal centre of Peyer's patches. In this study, we demonstrate that there are a number of differences in the localization of PrP(c) between the murine and bovine small intestines. PMID:17165097

  8. Study on cellular internalization of poly(vinyldiaminotriazine)-based hydrosen bonding type non-viral trans-gene vector

    Institute of Scientific and Technical Information of China (English)

    YE GuiXiang; CAO ZhiQiang; LIN Lin; CHEN DaYong; LIU WenGuang

    2008-01-01

    Previously we successfully prepared poly(vinyldiaminotriazine)(PVDT)-based non-viral vectors which complexed plasmid DNA via hydrogen bonding with adenine-thymine base pairs. In this report, surface charges and complex sizes of this system were further examined. The results showed that PVDT-based polymer could cover surface charges of DNA resulting in slightly negative or neutral complexes. It was also found that the complex sizes were governed by two events: the aggregation induced by the instability of neutral particles, and more compact complexes produced by PVDT-based polymers. In the study of cellular uptake, chlorpromazine and filipin III were used to inhibit clathrin- and caveolae-mediated endocytosis, respectively. We found that PVDT-based systems were transported into cells via a non-clathrin, non-caveolae mediated endocytosis. This special process was studied by temperature inhibition and kinetics assays. It was revealed that such a pathway was characterized by (i) a more energy dependent process and (ii) a much slow transfection-effective internalization.

  9. Cellular automata

    CERN Document Server

    Codd, E F

    1968-01-01

    Cellular Automata presents the fundamental principles of homogeneous cellular systems. This book discusses the possibility of biochemical computers with self-reproducing capability.Organized into eight chapters, this book begins with an overview of some theorems dealing with conditions under which universal computation and construction can be exhibited in cellular spaces. This text then presents a design for a machine embedded in a cellular space or a machine that can compute all computable functions and construct a replica of itself in any accessible and sufficiently large region of t

  10. Bone marrow involvement in diffuse large B-cell lymphoma: correlation between FDG-PET uptake and type of cellular infiltrate

    Energy Technology Data Exchange (ETDEWEB)

    Paone, Gaetano; Itti, Emmanuel; Lin, Chieh; Meignan, Michel [Universite Paris 12, Department of Nuclear Medicine, Hopital Henri Mondor, Assistance Publique-Hopitaux de Paris (AP-HP), Creteil (France); Haioun, Corinne; Dupuis, Jehan [Universite Paris 12, Department of Clinical Haematology, Hopital Henri Mondor, Assistance Publique-Hopitaux de Paris (AP-HP), Creteil (France); Gaulard, Philippe [Universite Paris 12, Department of Pathology, Hopital Henri Mondor, Assistance Publique-Hopitaux de Paris (AP-HP), Creteil (France); Universite Paris 12, INSERM U841, Hopital Henri Mondor, Assistance Publique-Hopitaux de Paris (AP-HP), Creteil (France)

    2009-05-15

    To assess, in patients with diffuse large B-cell lymphoma (DLBCL), whether the low sensitivity of {sup 18}F-fluorodeoxyglucose positron emission tomography (FDG-PET) for bone marrow assessment may be explained by histological characteristics of the cellular infiltrate. From a prospective cohort of 110 patients with newly diagnosed aggressive lymphoma, 21 patients with DLBCL had bone marrow involvement. Pretherapeutic FDG-PET images were interpreted visually and semiquantitatively, then correlated with the type of cellular infiltrate and known prognostic factors. Of these 21 patients, 7 (33%) had lymphoid infiltrates with a prominent component of large transformed lymphoid cells (concordant bone marrow involvement, CBMI) and 14 (67%) had lymphoid infiltrates composed of small cells (discordant bone marrow involvement, DBMI). Only 10 patients (48%) had abnormal bone marrow FDG uptake, 6 of the 7 with CBMI and 4 of the 14 with DBMI. Therefore, FDG-PET positivity in the bone marrow was significantly associated with CBMI, while FDG-PET negativity was associated with DBMI (Fisher's exact test, p=0.024). There were no significant differences in gender, age and overall survival between patients with CBMI and DBMI, while the international prognostic index was significantly higher in patients with CBMI. Our study suggests that in patients with DLBCL with bone marrow involvement bone marrow FDG uptake depends on two types of infiltrate, comprising small (DBMI) or large (CBMI) cells. This may explain the apparent low sensitivity of FDG-PET previously reported for detecting bone marrow involvement. (orig.)

  11. Cellular function and pathological role of ATP13A2 and related P-type transport ATPases in Parkinson's disease and other neurological disorders

    DEFF Research Database (Denmark)

    van Veen, Sarah; Sørensen, Danny M.; Holemans, Tine;

    2014-01-01

    . To discuss the role of ATP13A2 in neurodegeneration, we first provide a short description of the architecture and transport mechanism of P-type transport ATPases. Then, we briefly highlight key P-type ATPases involved in neuronal disorders such as the copper transporters ATP7A (Menkes disease), ATP7B (Wilson...... disease), the Na+/K+-ATPases ATP1A2 (familial hemiplegic migraine) and ATP1A3 (rapid-onset dystonia parkinsonism). Finally, we review the recent literature of ATP13A2 and discuss ATP13A2's putative cellular function in the light of what is known concerning the functions of other, better-studied P...

  12. E2 represses the late gene promoter of human papillomavirus type 8 at high concentrations by interfering with cellular factors.

    OpenAIRE

    Stubenrauch, F.; Leigh, I M; Pfister, H

    1996-01-01

    The late gene promoter P7535 of the epidermodysplasia verruciformis-associated human papillomavirus type 8 (HPV8) is regulated by the viral E2 protein. Transfection experiments performed with the human skin keratinocyte cell line RTS3b and P7535 reporter plasmids revealed transactivation at low amounts and a repression of basal promoter activity at high amounts of E2 expression vector. This repression was promoter specific and correlated with the amount of transiently expressed E2 protein. Mu...

  13. Acciones de promoción y fortalecimiento de la lactancia materna

    OpenAIRE

    Bernal Arias, Mariela; Orjuela Céspedes, Claudia Liliana

    2014-01-01

    Objetivo. Determinar las tendencias en las publicaciones sobre las acciones para la promoción y fortalecimiento de la lactancia materna durante en los últimos diez años. Metodología. Se realizó una revisión bibliográfica analítica, enfatizando en aspectos relacionados con conocimientos, actitudes y prácticas del personal de salud, ventajas y causas de abandono de la lactancia, conocimientos y prácticas de la comunidad, y políticas públicas para el fortalecimiento de la lactancia. Se consul...

  14. Impacto de las catástrofes en el valor de las acciones. El caso latinoamericano

    Directory of Open Access Journals (Sweden)

    Pedraza Martínez Alfonso

    2004-08-01

    Full Text Available La presente investigación aplicó la metodología de Knight y Pretty [1996] a catástrofes de seis empresas latinoamericanas para establecer cuál es el impacto de una catástrofe en el valor de las  acciones de tales firmas. En comparación con los resultados de Knight y Pretty [1996], en Latinoamérica la caída en el valor de las empresas en la bolsa tiene una pendiente menor que en USA y Europa, sin embargo, el impacto es más duradero.

  15. Los grandes eventos como acciones de relaciones públicas del Estado: las exposiciones universales

    OpenAIRE

    Otero Alvarado, María Teresa

    2007-01-01

    Las Exposiciones Universales constituyen una de las acciones más eficaces de Relaciones Públicas de los Estados. Ocasiones únicas para definir y mostrar la propia identidad, aparecen como plataformas comunicativas idóneas para transmitir sistemas de valores y planteamientos sociopolíticos y económicos, o recientemente, los principios de desarrollo y sostenibilidad imprescindibles hoy en el planeta Tierra. Analizamos la teoría general de las Exposiciones y en especial lo que supuso a efecto...

  16. Acciones de extensión, desde la universidad al medio

    OpenAIRE

    Gladys N. Dapozo; Mariño, Sonia Itatí; Godoy Guglielmone, María Viviana; López, María Victoria

    2009-01-01

    En el marco de la Sociedad de la Información, la capacitación en tecnologías de la Comunicación y la Información representa unaimportante estrategia como contribución al desarrollo local, potenciando la calidad de los recursos humanos y mejorando lascondiciones para la inserción laboral en jóvenes pertenecientes a sectores desfavorecidos. La Universidad Nacional del Nordestepromueve acciones de extensión al medio a través de programas específicos tendientes a: facilitar la inclusión social y ...

  17. ACCIONES PARA LA AUTORREGULACIÓN DEL APRENDIZAJE EN ENTORNOS PERSONALES

    OpenAIRE

    Eduardo Chaves Barboza; Juan Manuel Trujillo Torres; Juan Antonio López Núñez

    2016-01-01

    El objetivo del estudio es analizar las acciones que realizan los estudiantes de la población para la autorregulación del aprendizaje en entornos personales de aprendizaje. Se aplicó un cuestionario a una muestra de estudiantes de la Universidad de Granada (España) que pertenecen a las titulaciones de Pedagogía, Educación Social y Psicopedagogía. Sobre los datos se efectúan pruebas no paramétricas de correlaciones y análisis de varianzas, así como estadística descriptiva e inferencial. Los re...

  18. Acciones para la autorregulación del aprendizaje en entornos personales

    OpenAIRE

    Chaves Barboza, Eduardo; Trujillo Torres, Juan Manuel; López Núñez, Juan Antonio

    2016-01-01

    El objetivo del estudio es analizar las acciones que realizan los estudiantes de la población para la autorregulación del aprendizaje en entornos personales de aprendizaje. Se aplicó un cuestionario a una muestra de estudiantes de la Universidad de Granada (España) que pertenecen a las titulaciones de Pedagogía, Educación Social y Psicopedagogía. Sobre los datos se efectúan pruebas no paramétricas de correlaciones y análisis de varianzas, así como estadística descriptiva e inferencial. Los re...

  19. Wild-Type p53 Enhances Efficiency of Simian Virus 40 Large-T-Antigen-Induced Cellular Transformation▿

    OpenAIRE

    Hermannstädter, Andrea; Ziegler, Christine; Kühl, Marion; Deppert, Wolfgang; Tolstonog, Genrich V.

    2009-01-01

    Abortive infection of BALB/c mouse embryo fibroblasts differing in p53 gene status (p53+/+ versus p53−/−) with simian virus 40 (SV40) revealed a quantitatively and qualitatively decreased transformation efficiency in p53−/− cells compared to p53+/+ cells, suggesting a supportive effect of wild-type (wt) p53 in the SV40 transformation process. SV40 transformation efficiency also was low in immortalized p53−/− BALB/c 10-1 cells but could be restored to approximately the level in immortalized p5...

  20. Bovine papillomavirus type 1 E2 transcriptional regulators directly bind two cellular transcription factors, TFIID and TFIIB.

    OpenAIRE

    Rank, N M; Lambert, P F

    1995-01-01

    The bovine papillomavirus type 1 (BPV-1) E2 translational open reading frame encodes three proteins that regulate viral transcription and DNA replication: the E2 transcriptional activator (E2TA), the E2 transcriptional repressor (E2TR) and the E8/E2 transcriptional repressor (E8/E2TR). E2TA is a strong activator of papillomaviral promoters and is required for viral DNA replication. E2TR and E8/E2TR inhibit the activities of E2TA but also possess weak transactivational properties of their own....

  1. Transgenic Eimeria mitis expressing chicken interleukin 2 stimulated higher cellular immune response in chickens compared with the wild-type parasites

    Science.gov (United States)

    Li, Zhuoran; Tang, Xinming; Suo, Jingxia; Qin, Mei; Yin, Guangwen; Liu, Xianyong; Suo, Xun

    2015-01-01

    Chicken coccidiosis, caused by Eimeria sp., occurs in almost all poultry farms and causes huge economic losses in the poultry industry. Although this disease could be controlled by vaccination, the reduced feed conservation ratio limits the widespread application of anticoccidial vaccines in broilers because some intermediate and/or low immunogenic Eimeria sp. only elicit partial protection. It is of importance to enhance the immunogenicity of these Eimeria sp. by adjuvants for more effective prevention of coccidiosis. Cytokines have remarkable effects on the immunogenicity of antigens. Interleukin 2 (IL-2), for example, significantly stimulates the activation of CD8+ T cells and other immune cells. In this study, we constructed a transgenic Eimeria mitis line (EmiChIL-2) expressing chicken IL-2 (ChIL-2) to investigate the adjuvant effect of ChIL-2 to enhance the immunogenicity of E. mitis against its infection. Stable transfected EmiChIL-2 population was obtained by pyrimethamine selection and verified by PCR, genome walking, western blotting and indirect immunofluorescence assay. Cellular immune response, E. mitis-specific IFN-γ secretion lymphocytes in the peripheral blood mononuclear cells, stimulated by EmiChIL-2 was analyzed by enzyme-linked immunospot assay (ELISPOT). The results showed that EmiChIL-2 stimulated a higher cellular immune response compared with that of the wild-type parasite infection in chickens. Moreover, after the immunization with EmiChIL-2, elevated cellular immune response as well as reduced oocyst output were observed These results indicated that ChIL-2 expressed by Eimeria sp. functions as adjuvant and IL-2 expressing Eimeria parasites are valuable vaccine strains against coccidiosis. PMID:26082759

  2. Human immunodeficiency virus type 1 Tat increases the expression of cleavage and polyadenylation specificity factor 73-kilodalton subunit modulating cellular and viral expression.

    Science.gov (United States)

    Calzado, Marco A; Sancho, Rocío; Muñoz, Eduardo

    2004-07-01

    The human immunodeficiency virus type 1 (HIV-1) Tat protein, which is essential for HIV gene expression and viral replication, is known to mediate pleiotropic effects on various cell functions. For instance, Tat protein is able to regulate the rate of transcription of host cellular genes and to interact with the signaling machinery, leading to cellular dysfunction. To study the effect that HIV-1 Tat exerts on the host cell, we identified several genes that were up- or down-regulated in tat-expressing cell lines by using the differential display method. HIV-1 Tat specifically increases the expression of the cleavage and polyadenylation specificity factor (CPSF) 73-kDa subunit (CPSF3) without affecting the expression of the 160- and 100-kDa subunits of the CPSF complex. This complex comprises four subunits and has a key function in the 3'-end processing of pre-mRNAs by a coordinated interaction with other factors. CPSF3 overexpression experiments and knockdown of the endogenous CPSF3 by mRNA interference have shown that this subunit of the complex is an important regulatory protein for both viral and cellular gene expression. In addition to the known CPSF3 function in RNA polyadenylation, we also present evidence that this protein exerts transcriptional activities by repressing the mdm2 gene promoter. Thus, HIV-1-Tat up-regulation of CPSF3 could represent a novel mechanism by which this virus increases mRNA processing, causing an increase in both cell and viral gene expression.

  3. Anti-Aspergillus human host defence relies on type 1 T helper (Th1), rather than type 17 T helper (Th17), cellular immunity

    NARCIS (Netherlands)

    L.Y.A. Chai (Louis); F. van de Veerdonk (Frank); R.J. Marijnissen (Renoud); S.C. Cheng; A.L. Khoo; M. Hectors (Magda); K. Lagrou (Katrien); A.G. Vonk (Alieke); J. Maertens (Johan); L.A.B. Joosten (Leo); B.J. Kullberg (Bart Jan); M.G. Netea (Mihai)

    2010-01-01

    textabstractSummary Both interferon-γ-producing type 1 T helper (Th1)- and interleukin-17 (IL-17)-producing Th17 cells have been proposed to be involved in anti-fungal host defence. Although invasive aspergillosis is one of the most severe human fungal infections, little is known regarding the relat

  4. Anti-Aspergillus human host defence relies on type 1 T helper (Th1), rather than type 17 T helper (Th17), cellular immunity.

    NARCIS (Netherlands)

    Chai, L.; Veerdonk, F.L. van de; Marijnissen, R.J.; Cheng, S.C.; Khoo, A.L.; Hectors, M.P.C.; Lagrou, K.; Vonk, A.G.; Maertens, J.; Joosten, L.A.B.; Kullberg, B.J.; Netea, M.G.

    2010-01-01

    Both interferon-gamma-producing type 1 T helper (Th1)- and interleukin-17 (IL-17)-producing Th17 cells have been proposed to be involved in anti-fungal host defence. Although invasive aspergillosis is one of the most severe human fungal infections, little is known regarding the relative importance o

  5. La responsabilidad civil médica. Las llamadas acciones de Wrongful Birth y Wrongful Life

    Directory of Open Access Journals (Sweden)

    Andrea Macía Morillo

    2007-01-01

    Full Text Available La evolución de la responsabilidad civil ha llevado a la aparición de nuevas pretensiones de reparación de daños. En este trabajo se analizan dos de ellas, las acciones de wrongful birth y wrongful life, en las que se plantea la posibilidad de calificar la vida como daño ante el nacimiento de un niño afectado por enfermedades o malformaciones de las que no fueron informados los progenitores antes de la concepción o durante el embarazo. Este análisis se aborda desde una idea de continuidad en la aplicación de la institución de la responsabilidad civil, tratando de comprobar si concurren los presupuestos de la misma, sobre la idea de que sólo en caso afirmativo puede exigirse una indemnización. Desde esta perspectiva, los principales obstáculos a estas acciones son el daño reclamado y la imputación objetiva del mismo al profesional sanitario cuyo comportamiento sólo produjo el defecto en la información recibida por los progenitores

  6. Study the Effect of Echinacea Purpurea Extract on Cellular Delayed Type Hypersensitivity and Splenocyte Proliferation in BALB/c Mice

    Directory of Open Access Journals (Sweden)

    Z.M. Hassan

    2008-01-01

    Full Text Available Objective: Purple cone flower plant (Echinacea purpurea is one of the mostimportant Herbal products in many countries. Up to now a lot of experimentsdemonstrated the controversial effects of this herb on immune system . In thisresearch we study the in vivo and in vitro effect of Iranian E.purpurea extract oncellular immunity.Materials and Methods: At first we determined the lethal dose of E.purpurea extract after intraperitoneal injection in BALB/c mice. Then we made five groups of mice and treat them by four times intraperitoneal injection of 1 ml extract at different doses (0, 0.4, 2, 10 and 50 mg/ml during two weeks. Splenocyte proliferation response to extract was assessed by MTT method. Delayed-Type Hypersensitivity (DTHresponse was evaluated by priming mice with 1×108 Sheep Red Blood Cell (SRBC injected subcutaneously in the back on day 7after treatment.Results: As a result no significant variation in weight and spleen index of test groups to control was observed. Splenocyte proliferation and DTH response of test groups to control increased significantly (p<0.05.Conclusion: However these data confirmed the results of previous studies, inaddition presented the first results about significant increase in DTH response that could not be seen before. Scince the main reason of this difference refers to active compounds of herb extract, comparing effective component of this extract with that of E.purpurea cultivated in other geographical condition will consider as the next studies.

  7. IL-17-dependent cellular immunity to collagen type V predisposes to obliterative bronchiolitis in human lung transplants.

    Science.gov (United States)

    Burlingham, William J; Love, Robert B; Jankowska-Gan, Ewa; Haynes, Lynn D; Xu, Qingyong; Bobadilla, Joseph L; Meyer, Keith C; Hayney, Mary S; Braun, Ruedi K; Greenspan, Daniel S; Gopalakrishnan, Bagavathi; Cai, Junchao; Brand, David D; Yoshida, Shigetoshi; Cummings, Oscar W; Wilkes, David S

    2007-11-01

    Bronchiolitis obliterans syndrome (BOS), a process of fibro-obliterative occlusion of the small airways in the transplanted lung, is the most common cause of lung transplant failure. We tested the role of cell-mediated immunity to collagen type V [col(V)] in this process. PBMC responses to col(II) and col(V) were monitored prospectively over a 7-year period. PBMCs from lung transplant recipients, but not from healthy controls or col(IV)-reactive Goodpasture's syndrome patients after renal transplant, were frequently col(V) reactive. Col(V)-specific responses were dependent on both CD4+ T cells and monocytes and required both IL-17 and the monokines TNF-alpha and IL-1beta. Strong col(V)-specific responses were associated with substantially increased incidence and severity of BOS. Incidences of acute rejection, HLA-DR mismatched transplants, and induction of HLA-specific antibodies in the transplant recipient were not as strongly associated with a risk of BOS. These data suggest that while alloimmunity initiates lung transplant rejection, de novo autoimmunity mediated by col(V)-specific Th17 cells and monocyte/macrophage accessory cells ultimately causes progressive airway obliteration. PMID:17965778

  8. Cellular Cardiomyoplasty: Clinical Application

    OpenAIRE

    Chachques, J. (J.); Acar, C; J. Herreros; Trainini, J. (Jorge); Prosper, F.; D’Attellis, N. (N.); Fabiani, J. N.; Carpentier, A

    2004-01-01

    Myocardial regeneration can be induced with the implantation of a variety of myogenic and angiogenic cell types. More than 150 patients have been treated with cellular cardiomyoplasty worldwide, 18 patients have been treated by our group. Cellular cardiomyoplasty seems to reduce the size and fibrosis of infarct scars, limit postischemic remodelling, and restore regional myocardial contractility. Techniques for skeletal myoblasts culture and ex vivo expansion using auto...

  9. De los legitimados para presentar acciones por competencia desleal, los tipos de acciones, su prescripción y medidas cautelares

    Directory of Open Access Journals (Sweden)

    Dionisio M. de la Cruz Camargo

    2008-08-01

    Full Text Available La Ley 256 de 1996 reguló de manera novedosa el tema de la competencia desleal y no sólo en lo atinente a definir qué se debe entender por competencia desleal y cuáles son los comportamientos que según la ley colombiana son considerados como desleales, sino que también reglamentó aspectos procesales de no poca trascendencia que marcan la evolución legislativa en la materia. La ampliación de las personas legitimadas para interponer acciones por competencia desleal es uno de los avances más destacados de la legislación vigente en Colombia. El hecho de que no sólo los comerciantes, como profesionales en la actividad mercantil, puedan interponer una demanda por competencia desleal contra el competidor directo al que se le impute la comisión de un acto desleal, implica que se ha desprovisto el carácter profesional y corporativo a la aplicación de la normatividad en la actividad económica y se ha reconocido la trascendencia de las conductas de competencia desleal en la sociedad y la economía, las cuales dejan de ser simples comportamientos que perjudican intereses privados individuales para convertirse en conductas que pueden afectar el mercado y sus partícipes. Por un lado, tenemos a los consumidores, quienes no sólo son los árbitros de la actividad comercial al decidir con su elección entre los bienes y servicios que se le ofrecen, el que mejor colme sus expectativas de consumo, sino que también se les reconoce su condición de potenciales perjudicados por la comisión de actos desleales, por lo que pueden reclamar los perjuicios que dichos actos le irroguen, cuando estos hayan afectado a todo un conjunto de consumidores asociados en un ente jurídico que proteja sus intereses. esto no es óbice para que un consumidor individual no pueda ejercer una acción por los perjuicios que un comportamiento le pueda causar. Del otro, las corporaciones y asociaciones gremiales, ya que el legislador consideró que era loable proteger el

  10. Expression of human papilloma virus type 16 E5 protein in amelanotic melanoma cells regulates endo-cellular pH and restores tyrosinase activity

    Directory of Open Access Journals (Sweden)

    Coccia Raffaella

    2009-01-01

    Full Text Available Abstract Background Melanin synthesis, the elective trait of melanocytes, is regulated by tyrosinase activity. In tyrosinase-positive amelanotic melanomas this rate limiting enzyme is inactive because of acidic endo-melanosomal pH. The E5 oncogene of the Human Papillomavirus Type 16 is a small transmembrane protein with a weak transforming activity and a role during the early steps of viral infections. E5 has been shown to interact with 16 kDa subunit C of the trans-membrane Vacuolar ATPase proton pump ultimately resulting in its functional suppressions. However, the cellular effects of such an interaction are still under debate. With this work we intended to explore whether the HPV16 E5 oncoprotein does indeed interact with the vacuolar ATPase proton pump once expressed in intact human cells and whether this interaction has functional consequences on cell metabolism and phenotype. Methods The expression of the HPV16-E5 oncoproteins was induced in two Tyrosinase-positive amelanotic melanomas (the cell lines FRM and M14 by a retroviral expression construct. Modulation of the intracellular pH was measured with Acridine orange and fluorescence microscopy. Expression of tyrosinase and its activity was followed by RT-PCR, Western Blot and enzyme assay. The anchorage-independence growth and the metabolic activity of E5 expressing cells were also monitored. Results We provide evidence that in the E5 expressing cells interaction between E5 and V-ATPase determines an increase of endo-cellular pH. The cellular alkalinisation in turn leads to the post-translational activation of tyrosinase, melanin synthesis and phenotype modulation. These effects are associated with an increased activation of tyrosine analogue anti-blastic drugs. Conclusion Once expressed within intact human cells the HPV16-E5 oncoprotein does actually interact with the vacuolar V-ATPase proton pump and this interaction induces a number of functional effects. In amelanotic melanomas these

  11. Activation of Cellular Immunity in Herpes Simplex Virus Type 1-Infected Mice by the Oral Administration of Aqueous Extract of Moringa oleifera Lam. Leaves.

    Science.gov (United States)

    Kurokawa, Masahiko; Wadhwani, Ashish; Kai, Hisahiro; Hidaka, Muneaki; Yoshida, Hiroki; Sugita, Chihiro; Watanabe, Wataru; Matsuno, Koji; Hagiwara, Akinori

    2016-05-01

    Moringa oleifera Lam. is used as a nutritive vegetable and spice. Its ethanol extract has been previously shown to be significantly effective in alleviating herpetic skin lesions in mice. In this study, we evaluated the alleviation by the aqueous extract (AqMOL) and assessed the mode of its anti-herpetic action in a murine cutaneous herpes simplex virus type 1 (HSV-1) infection model. AqMOL (300 mg/kg) was administered orally to HSV-1-infected mice three times daily on days 0 to 5 after infection. AqMOL significantly limited the development of herpetic skin lesions and reduced virus titers in the brain on day 4 without toxicity. Delayed-type hypersensitivity (DTH) reaction to inactivated HSV-1 antigen was significantly stronger in infected mice administered AqMOL and AqMOL augmented interferon (IFN)-γ production by HSV-1 antigen from splenocytes of HSV-1-infected mice at 4 days post-infection. AqMOL administration was effective in elevating the ratio of CD11b(+) and CD49b(+) subpopulations of splenocytes in infected mice. As DTH is a major host defense mechanism for intradermal HSV infection, augmentation of the DTH response by AqMOL may contribute to their efficacies against HSV-1 infection. These results provided an important insights into the mechanism by which AqMOL activates cellular immunity. Copyright © 2016 John Wiley & Sons, Ltd. PMID:26814058

  12. Accions enfront la crisi i baixada de vendes d’un negoci de Fleca/Cafeteria

    OpenAIRE

    Civit Cardelús, Albert

    2014-01-01

    La situació actual de crisi arriba a tots els àmbits i sectors industrials, i no dóna més opció a les empreses que reinventar-se, prendre noves acciones i adaptar-se al nou escenari i fer-ho de la manera més competitiva possible. D’aquesta manera, fins i tot en negocis de tota la vida de venda de pa com és el cas de l’empresa “La Panera”, sorgeix la necessitat de buscar vies d’innovació per tal d’adaptar-se a la conjuntura econòmica actual i preparar-se per a les noves tendències que deparin ...

  13. El 9 de Abril en Cali y en el Valle: Acciones De La Muchedumbre

    Directory of Open Access Journals (Sweden)

    Darío Betancourt Echeverry

    2012-08-01

    Full Text Available En el gaitanismo, fuera por convicción o manipulación política, encontraron continuidad y refugio grandes sectores populares, provenientes de una tradición campesino-artesanal. Por ello el autor deja planteado que las "acciones de la muchedumbre" caleña el 9 de abril de 1948, deben entenderse no como la actuación de una turba salvaje, sino como la continuidad de una protesta popular con raigambre ancestral. Se hace énfasis en el papel desempeñado por el entonces teniente Gustavo Rojas Pinilla, en la consumación del desorden y en la vuelta a la normalidad.

  14. Responsabilidad por violación de la buena fe en la sociedad por acciones simplificada

    Directory of Open Access Journals (Sweden)

    Nicolás Polanía Tello

    2012-12-01

    Full Text Available La sociedad por acciones simplificada –SAS–, como los demás esquemas societarios, se funda en y está informada por el principio de la buena fe. Pero a diferencia de los otros modelos, la SAS tiene concreciones particulares de la buena fe que permiten un amparo más efectivo y eficiente de los derechos de los socios y de los terceros, en la medida en que instituciones como el fraude a la ley, el perjuicio injusto a terceros, el abuso del derecho y el administrador de hecho, todos estructurados a partir de violaciones a la buena fe, permiten tramitar los eventos de responsabilidad por ilícitos atípicos y llegar incluso a desestimar por ello el atributo de la limitación de responsabilidad.

  15. Cellular Telephone

    Institute of Scientific and Technical Information of China (English)

    杨周

    1996-01-01

    Cellular phones, used in automobiles, airliners, and passenger trains, are basically low-power radiotelephones. Calls go through radio transmitters that are located within small geographical units called cells. Because each cell’s signals are too weak to interfere with those of other cells operating on the same fre-

  16. TRIM32 protein modulates type I interferon induction and cellular antiviral response by targeting MITA/STING protein for K63-linked ubiquitination.

    Science.gov (United States)

    Zhang, Jing; Hu, Ming-Ming; Wang, Yan-Yi; Shu, Hong-Bing

    2012-08-17

    Viral infection activates several transcription factors including NF-κB and IRF3, which collaborate to induce type I interferons (IFNs) and innate antiviral response. MITA (also called STING) is a critical adaptor protein that links virus-sensing receptors to IRF3 activation upon infection by both RNA and DNA pathogens. Here we show that the E3 ubiquitin ligase tripartite motif protein 32 (TRIM32) ubiquitinated MITA and dramatically enhanced MITA-mediated induction of IFN-β. Overexpression of TRIM32 potentiated virus-triggered IFNB1 expression and cellular antiviral response. Consistently, knockdown of TRIM32 had opposite effects. TRIM32 interacted with MITA, and was located at the mitochondria and endoplasmic reticulum. TRIM32 targeted MITA for K63-linked ubiquitination at K20/150/224/236 through its E3 ubiquitin ligase activity, which promoted the interaction of MITA with TBK1. These findings suggest that TRIM32 is an important regulatory protein for innate immunity against both RNA and DNA viruses by targeting MITA for K63-linked ubiquitination and downstream activation.

  17. DIFFERENTIAL BINDING OF HUMAN INTERLEUKIN-1 (IL-1) RECEPTOR ANTAGONIST TO NATURAL AND RECOMBINANT SOLUBLE AND CELLULAR IL-1 TYPE-I RECEPTORS

    DEFF Research Database (Denmark)

    Svenson, Morten; Nedergaard, Susanne; Heegaard, Peter M. H.;

    1995-01-01

    A recently described factor, interleukin-1 receptor antagonist binding factor (IL-1raBF), in serum of normal individuals is immunologically related to the interleukin-1 receptor type I (IL-1RI). It is presumably a soluble form of the receptor that binds exclusively to interleukin-1 receptor...... antagonist (IL-1ra). Recombinant soluble human IL-1RI expressed in COS cells (sIL-1RI) consists of the extracellular part of the receptor and binds all three known IL-1 species but preferentially to IL-1ra. We further characterized the sizes and binding of IL-1raBF and sIL-1RI to IL-1ra by polyacrylamide gel...... electrophoresis in the presence of sodium dodecylsulfate, ligand binding interference analyses, N-glycosidase treatment, concanavalin A affinity chromatography, and with the use of monoclonal antibodies (mAb) to human recombinant IL-1ra. We also evaluated the binding of IL-1ra to cellular IL-1RI on MRC5...

  18. Efficient Cellular Entry of (r-x-r)-Type Carbamate-Plasmid DNA Complexes and Its Implication for Noninvasive Topical DNA Delivery to Skin.

    Science.gov (United States)

    Vij, Manika; Natarajan, Poornemaa; Yadav, Amit K; Patil, Kiran M; Pandey, Tanuja; Gupta, Nidhi; Santhiya, Deenan; Kumar, Vaijayanti A; Fernandes, Moneesha; Ganguli, Munia

    2016-06-01

    Arginine-rich cell penetrating peptides are powerful tools for in vitro as well as in vivo delivery of a wide plethora of biomolecules. However, presence of consecutive arginine residues leads to enhanced amenability for proteolytic degradation as well as steric hindrances for membrane interactions which compromise its bioavailability. In order to overcome these limitations we previously reported a safe and stable octaarginine based oligomer, i.e., (r-x-r)4-carbamate, where the backbone amide linkages were replaced by carbamate linkages and 6-aminohexanoic acid based spacer moieties were incorporated for better flexibility, hydrophobicity, optimal spacing of guanidinium groups, and protection against proteolytic cleavage; resulting in improved transfection efficiency over its amide counterpart. In the present work we have investigated the mechanism behind this enhanced transfection efficiency and, based on our observations, demonstrate how the synergistic effect of rationalized oligomer designing, complex characteristics, and cell type contributes to overall effective intracellular delivery. Our results indicate that the (r-x-r)4-carbamate-plasmid DNA complexes primarily utilize lipid raft dependent pathway of cellular entry more than other pathways, and this possibly facilitates their increased entry in the lipid raft rich milieu of skin cells. We also emphasize the utility of oligomer (r-x-r)4-carbamate as an efficient carrier for topical delivery of nucleic acids in skin tissue. This carrier can be utilized for safe, efficient, and noninvasive delivery of therapeutically relevant macromolecular hydrophilic cargo like nucleic acids to skin. PMID:27175623

  19. De los legitimados para presentar acciones por competencia desleal, los tipos de acciones, su prescripción y medidas cautelares

    Directory of Open Access Journals (Sweden)

    Dionisio M. de la Cruz Camargo

    2008-08-01

    Full Text Available La Ley 256 de 1996 reguló de manera novedosa el tema de la competencia desleal y no sólo en lo atinente a definir qué se debe entender por competencia desleal y cuáles son los comportamientos que según la ley colombiana son considerados como desleales, sino que también reglamentó aspectos procesales de no poca trascendencia que marcan la evolución legislativa en la materia. La ampliación de las personas legitimadas para interponer acciones por competencia desleal es uno de los avances más destacados de la legislación vigente en Colombia. El hecho de que no sólo los comerciantes, como profesionales en la actividad mercantil, puedan interponer una demanda por competencia desleal contra el competidor directo al que se le impute la comisión de un acto desleal, implica que se ha desprovisto el carácter profesional y corporativo a la aplicación de la normatividad en la actividad económica y se ha reconocido la trascendencia de las conductas de competencia desleal en la sociedad y la economía, las cuales dejan de ser simples comportamientos que perjudican intereses privados individuales para convertirse en conductas que pueden afectar el mercado y sus partícipes. Por un lado, tenemos a los consumidores, quienes no sólo son los árbitros de la actividad comercial al decidir con su elección entre los bienes y servicios que se le ofrecen, el que mejor colme sus expectativas de consumo, sino que también se les reconoce su condición de potenciales perjudicados por la comisión de actos desleales, por lo que pueden reclamar los perjuicios que dichos actos le irroguen, cuando estos hayan afectado a todo un conjunto de consumidores asociados en un ente jurídico que proteja sus intereses. esto no es óbice para que un

  20. Cytotoxicity and cellular uptake of pyrimidine nucleosides for imaging herpes simplex type-1 thymidine kinase (HSV-1 TK) expression in mammalian cells

    Energy Technology Data Exchange (ETDEWEB)

    Morin, Kevin W.; Duan Weili; Xu Lihua; Zhou Aihua; Moharram, Sameh; Knaus, Edward E.; McEwan, Alexander J.B.; Wiebe, Leonard I. E-mail: leonard.wiebe@ualberta.ca

    2004-07-01

    In vivo transfer of the herpes simplex virus type-1 thymidine kinase (HSV-1 TK) gene, with subsequent administration of antiviral drugs such as ganciclovir, has emerged as a promising gene therapy protocol for treating proliferative disorders. The in vitro cytotoxicities (IC{sub 50}) for two series of 5-iodo- and (E)-5-(2-iodovinyl)-substituted 2'-deoxy- and 2'-deoxy-2'-fluoro-pyrimidine nucleosides ranged from millimolar to low nanomolar concentrations in mammalian tumor cell lines (KBALB; R-970-5; 143B; EMT-6) and their counterparts engineered to express HSV-1 TK (KBALB-STK; 143B-LTK). Their HSV-1 TK selectivity indices ranged from one (nonselective) to one million (highly selective) based on cytotoxicity, with FIRU being the least toxic to all cell lines, and FIAU being most toxic. HSV-1 TK selectivity, based on uptake, ranged from 10 to 140, with IVDU being most selective for HSV-1 TK expressing cells, followed by IVFRU, FIRU, FIAU, IVFAU and finally IUDR. Phosphorylation of [{sup 125}I]FIAU led to incorporation of the radiolabel into nucleic acids, whereas IVFRU and FIRU radioactivity was trapped primarily in the nucleotide pool. These data indicate that cytotoxicity does not depend on initial metabolic trapping (e.g., phosphorylation), but on elaboration of the mononucleotides to more cytotoxic anabolites. Lipophilicities and nucleoside transport rates of the six nucleosides tested were within narrow ranges. This supports the premise that cellular biochemistry, and not cellular bioavailability, is responsible for the observed broad range of cytotoxicity and trapping. In vivo biodistribution studies with 5-[{sup 125}I]iodo-2'-fluoro-2'-deoxyribouridine (FIRU), 5-[{sup 125}I]iodo-2'-fluoro-2'-deoxyarabinouridine (FIAU) and (E)-5-(2-[{sup 125}I]iodovinyl)-2'-fluoro-2'-deoxyuridine (IVFRU) demonstrate selective accumulation of all three radiotracers in HSV-1 TK-expressing KBABK-STK tumors, compared to their very low

  1. The State of Cellular Probes

    OpenAIRE

    Yim, Youngbin

    2003-01-01

    Cellular probe technology is one of several potentially promising technologies for obtaining accurate travel time information. In 1996, the Federal Communications Commission (FCC) mandated E911 requirements that cellular location be provided when 911 emergency calls come in to emergency management authorities. The E911 requirements allow 50 -300 meters from the emergency call location, depending on the type of cellular phone technology used and whether handset-based or network-based solutions...

  2. Cellular Phone Towers, Wireless Towers data layer - not sure of the tower type included, Published in 2008, 1:63360 (1in=1mile) scale, Graham County.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Cellular Phone Towers dataset, published at 1:63360 (1in=1mile) scale, was produced all or in part from Not Provided information as of 2008. It is described as...

  3. Argentina-Brasil: primeras acciones coordinadas en política exterior

    Directory of Open Access Journals (Sweden)

    Daniel Amicci

    2012-01-01

    Full Text Available El abordaje de las relaciones argentino-brasileñas durante el desarrollismo abre el camino para realizar una interpretación amplia de la articulación de una y otra Cancillería en pos de objetivos comunes. El presente artículo describe los resultados alcanzados luego de las negociaciones de la cumbre de Uruguayana de 1961, que dieron lugar a acciones recíprocas tendientes a lograr, por un lado, la implementación de los créditos norteamericanos hacia las áreas productivas y, por el otro, a la continuación de una política exterior independiente, que debió lidiar con las presiones del gobierno del Norte para excluir a Cuba del sistema de naciones americanas.En ese sentido dialogamos, sobre todo, acerca de las medidas tomadas por ambas diplomacias en Punta del Este (1962, cuyas derivaciones alimentaron los movimientos golpistas que condujeron a una nueva involución en el proceso de convergencia entre ambos vecinos del Cono Sur.

  4. REFLEXIONES SOBRE LAS DIFICULTADES INSTITUCIONALES PARA REALIZAR ACCIONES EN LA GESTIÓN DEL RIESGO

    Directory of Open Access Journals (Sweden)

    Leoz M. Francisco Javier

    2008-07-01

    Full Text Available La gestión del riesgo en Colombia se estructura bajo un conjunto de normas que definen a los actores dentro de un esquema de niveles jerárquicos donde algunas instituciones del Estado adquieren, además de sus funciones misionales, la administración del tema de prevención de desastres. La misma normatividad crea obstáculos entre las instituciones que limitan los esfuerzos en la reducción del riesgo. Entre las barreras identificadas, se plantean los límites jurisdiccionales que imposibilitan acciones de inversión en sectores donde un escenario de riesgo está dividido por una línea imaginaria que separa la actuación entre entes territoriales o entre autoridades ambientales. Otra de las barreras es la estructura del Sistema Nacional de Prevención y Atención de Desastres que descarga toda la responsabilidad a los municipios; asimismo la normatividad y el manejo de la información técnica se consideran como barreras para la adecuada gestión del riesgo.

  5. Program of actions for energy conservation in the equipment for the urban public transportation; Programa de acciones para el ahorro de la energia en los equipos de transporte publico urbanos

    Energy Technology Data Exchange (ETDEWEB)

    Sanhez Arellano, L.I. [Facultad de Ingenieria, Universidad Autonoma del Estado de Mexico, Toluca (Mexico)

    1995-12-31

    The Facultad de Ingenieria of the Universidad Autonoma del Estado de Mexico (UAEM), in its postgraduate program in Transport Engineering carries out research projects aimed at the appropriate operation of the urban public transportation, in the definition of the transportation networks and their exploitation as well as in the energy conservation and efficient use of the energy for its operation. This paper embodies the lines of interest of this program. The proper operation characteristics and the energy consumption of the different types of vehicles used in urban public transportation mainly depend on the application they have been designed for. Nevertheless, an important point is to know, in the most concrete and specific form, the nature and other possible utilization options of the different urban transportation systems, as well as its energy impact and the possibility of a well oriented energy conservation. The purpose of this paper is to present in a concrete manner the different types of vehicles used in the urban public transportation, making an energy comparison, besides setting forth a program of actions tending to the energy saving for the vehicles that nowadays operate in the main cities of our country. The main actions are pointed out and the basic characteristics for their initiation, which can be summarized in three large groups: -action in the proper enterprises and with existing vehicular park. -actions over the management and exploitation of the transportation systems. -action for the utilization of new equipment and more efficient vehicles. A detailed explanation of each one of these actions and their impact on the energy consumption are the final purpose of this study. [Espanol] La Facultad de Ingenieria de la Universidad Autonoma del Estado de Mexico (UAEM), dentro de su programa de Postrado en Ingenieria del Transporte realiza proyectos de investigacion tendientes en la buena operacion de los transportes publicos urbanos, en la definicion de

  6. Actual problems of cellular cardiomyoplasty

    Directory of Open Access Journals (Sweden)

    Bulat Kaupov

    2010-04-01

    Full Text Available The paper provides review of cellular technologies used incardiology, describes types of cellular preparations depending onsources of cells and types of compounding cells. The generalmechanisms of therapies with stem cells applications are described.Use of cellular preparations for treatment of cardiovascular diseasesand is improvement of the forecast at patients with heartinsufficiency of various genesis is considered as alternative topractice with organ transplantations. Efforts of biotechnologicallaboratories are directed on search of optimum population of cellsfor application in cardiology and studying of mechanisms andfactors regulating function of cardiac stem cells.

  7. Elimination of human T cell leukemia virus type-1-infected cells by neutralizing and antibody-dependent cellular cytotoxicity-inducing antibodies against human t cell leukemia virus type-1 envelope gp46.

    Science.gov (United States)

    Tanaka, Yuetsu; Takahashi, Yoshiaki; Tanaka, Reiko; Kodama, Akira; Fujii, Hideki; Hasegawa, Atsuhiko; Kannagi, Mari; Ansari, Aftab A; Saito, Mineki

    2014-06-01

    Human T cell leukemia virus type-1 (HTLV-1) is prevalent worldwide with foci of high prevalence. However, to date no effective vaccine or drug against HTLV-1 infection has been developed. In efforts to define the role of antibodies in the control of HTLV-1 infection, we capitalized on the use of our previously defined anti-gp46 neutralizing monoclonal antibody (mAb) (clone LAT-27) and high titers of human anti-HTLV-1 IgG purified from HAM/TSP patients (HAM-IgG). LAT-27 and HAM-IgG completely blocked syncytium formation and T cell immortalization mediated by HTLV-1 in vitro. The addition of these antibodies to cultures of CD8(+) T cell-depleted peripheral blood mononuclear cells (PBMCs) from HAM/TSP patients at the initiation of culture not only decreased the numbers of Tax-expressing cells and the production of HTLV-1 p24 but also inhibited the spontaneous immortalization of T cells. Coculture of in vitro-HTLV-1-immortalized T cell lines with autologous PBMCs in the presence of LAT-27 or HAM-IgG, but not an F(ab')2 fragment of LAT-27 or nonneutralizing anti-gp46 mAbs, resulted in depletion of HTLV-1-infected cells. A 24-h (51)Cr release assay showed the presence of significant antibody-dependent cellular cytotoxicity (ADCC) activity in LAT-27 and HAM-IgG, but not F(ab')2 of LAT-27, resulting in the depletion of HTLV-1-infected T cells by autologous PBMCs. The depletion of natural killer (NK) cells from the effector PBMCs reduced this ADCC activity. Altogether, the present data demonstrate that the neutralizing and ADCC-inducing activities of anti-HTLV-1 antibodies are capable of reducing infection and eliminating HTLV-1-infected cells in the presence of autologous PBMCs. PMID:24524420

  8. Utilización de la prensa escrita como medio de divulgación de las acciones de RSE

    Directory of Open Access Journals (Sweden)

    Omaira Cecilia Martínez Moreno

    2012-12-01

    Full Text Available El tema de la Responsabilidad Social Empresarial –RSE– ha hecho que las empresas se sensibilicen en torno a su nivel de responsabilidad social, cultural, económica y ambiental, entre otros. Esta investigación aborda precisamente estas acciones y su nivel de divulgación en un contexto en particular. Inicialmente se planteó como descriptiva y persiguió hacer un estudio sobre la caracterización tanto cualitativa como cuantitativa de la información inherente a la Responsabilidad Social Empresarial que se publica en el periódico Frontera, de la ciudad de Tijuana, Baja California, México. Se seleccionó este periódico por ser un medio de comunicación líder absoluto en su rubro en la ciudad. En la investigación de campo se establecieron acciones desarrolladas por las empresas en tres grandes áreas: social, ambiental y económica. En rasgos generales, se evidencia una ausencia de cultura hacia la publicación de las acciones que en torno a la RSE se implementan y desarrollan. Las empresas que más publican tanto noticias como publicidad son de carácter público. Las de carácter privado, probablemente sí desarrollen algún tipo de actividad en este sentido, pero desconocen el impacto que la comunicación de éstas puede tener en la percepción de los consumidores actuales o potenciales.

  9. Utilización de la prensa escrita como medio de divulgación de las acciones de RSE

    OpenAIRE

    Omaira Cecilia Martínez Moreno; José Gabriel Ruiz Andrade; Ricardo Verján Quiñones

    2012-01-01

    El tema de la Responsabilidad Social Empresarial –RSE– ha hecho que las empresas se sensibilicen en torno a su nivel de responsabilidad social, cultural, económica y ambiental, entre otros. Esta investigación aborda precisamente estas acciones y su nivel de divulgación en un contexto en particular. Inicialmente se planteó como descriptiva y persiguió hacer un estudio sobre la caracterización tanto cualitativa como cuantitativa de la información inherente a la Responsabilidad Social Empresaria...

  10. Relación entre teoría de la justicia y acciones no-violentas

    OpenAIRE

    María del Rosario Guerra González

    2013-01-01

    Este texto tiene tres partes: en primer término se caracterizan diversas formas de acciones no-violentas, en segundo término se presenta la justificación de la desobediencia civil dentro de la teoría de Rawls y en tercera parte se amplía el problema del derecho interno de los Estados al sistema internacional. Por motivos de justicia cada día son más las situaciones que han provocado desobediencia civil. Primero fue la defensa de derechos civiles y políticos quien causó la resistencia, luego l...

  11. Reflexiones sobre arte para lo político y representación en las acciones del colectivo LULI

    OpenAIRE

    Fukelman, María Cristina; Di María, Graciela; Sánchez Pórfido, Elisabet; González, Silvia Juana; Albero, María Marta; Gatica, Danisa; Naón, Marisa; Ladaga, Cristian; Montequin, Diana; Estévez, Mariana

    2013-01-01

    Esta ponencia tiene como finalidad reflexionar sobre determinados conceptos, de diferentes densidades semánticas, que designan, explican y analizan modalidades de producción artística centradas en espacios alternativos, que dan la espalda a los espacios y medios institucionales tradicionales del campo artístico. Se referirá a algunas acciones del colectivo LULI. Entre los conceptos que se trabajaran se encuentra el arte político, arte para lo político, arte público, arte de acción, activismo,...

  12. Interpretaciones y aportes recientes sobre las acciones colectivas frente a la violencia y el conflicto armado en Colombia

    Directory of Open Access Journals (Sweden)

    Mary Luz Alzate Zuluaga

    2010-01-01

    Full Text Available En este artículo se examina el tema de las acciones colectivas producidas para enfrentar la violencia y el conflicto armado en Colombia, por un lado en la bibliografía especializada en el tema de la violencia y el conflicto armado y, por otro, en la bibliografía dedicada al análisis del movimiento social por la paz y los derechos humanos. En primer lugar, se mencionan las apreciaciones y generalizaciones sobre el comportamiento ciudadano y la cultura política frente a las situaciones de violencia y conflicto armado. En segundo lugar, los principales aportes sobre el surgimiento y dinámica nacional de los movimientos sociales por la paz y los derechos humanos. Y, en tercer lugar, los aportes recientes de los estudios que han descrito y analizado distintas acciones colectivas locales, algunas a modo de experiencias y manifestaciones colectivas de resistencia civil, otras como acumulados sociales y políticos frente a la violencia producida producida en el desarrollo del conflicto interno armado. La conclusión es que los actores colectivos se han movilizado pese a las amenazas en contra de la vida y la integridad física, evidenciando en el examen reciente del tema, el carácter momentáneo y relativo de la subordinación civil frente al poder armado y despótico.

  13. Comentarios en torno al allanamiento de La personalidad jurídica en las sociedades por acciones simplificadas

    Directory of Open Access Journals (Sweden)

    Camilo Enrique Cubillos Garzón

    2009-12-01

    Full Text Available Con el fin de generar una postura crítica frente a la adaptación de nuevas figuras jurídicas en nuestro ordenamiento, el autor hace un recorrido por los fundamentos y finalidades de la Teoría de la Desestimación de la Personalidad Jurídica y su aplicación en el caso de la Sociedad por Acciones Simplificadas. Lo anterior, en la medida que en opinión del autor, la consagración de la posibilidad de levantar el velo de estos sociedades contenida en el artículo 42 de la ley 1258 de 2008, en contra de los socios y los administradores comporta un desconocimiento de la naturaleza jurídica y finalidad de esta figura y de otras acciones existentes en nuestro ordenamiento apropiadas para perseguir la responsabilidad de los administradores, como lo son la acción social e individual de responsabilidad.

  14. Acciones colectivas contenciosas, proceso político y seguridad urbana. Construyendo geografías de la esperanza

    Directory of Open Access Journals (Sweden)

    Luis Berneth Peña

    2014-09-01

    Full Text Available Este artículo describe algunos repertorios y redes de acciones colectivas contenciosas (acciones popu - lares que buscan construir o profundizar derechos desplegadas por algunas organizaciones sociales en Bogotá que promueven formas no autoritarias de seguridad urbana. Basado en una perspectiva espacial de la acción colectiva, se enfatiza en el lugar de la política popular en los procesos políticos y la política de lugar en la que se construyen nuevas formas de solidaridad fundamentadas en arrai - gos comunitarios. En la primera sección, se expone el concepto de acción colectiva contenciosa. La segunda describe la estructura espacial de la acción colectiva de las organizaciones sociales en dos escalas: a la escala de una localidad (Ciudad Bolívar y b la escala de un proyecto donde convergen varias organizaciones en el barrio Los Libertadores en la localidad de San Cristóbal. En la tercera, se presentan algunos ejemplos del repertorio de confrontación de las organizaciones. En la última parte, se discuten los resultados encontrados.

  15. Velocidad de ajuste del precio de las acciones por sector económico en la Bolsa Mexicana de Valores

    Directory of Open Access Journals (Sweden)

    Verónica Patricia Rodríguez Vázquez

    2009-01-01

    Full Text Available Este estudio analiza la velocidad de ajuste del precio de las acciones de la Bolsa Mexicana de Valores de enero de 1998 a diciembre de 2007; para ello, se agruparon las acciones por sectores económicos y se compararon con el Índice de Precios y Cotizaciones (IPC. Asimismo, se clasificó a los sectores económicos en fuertes y débiles, dependiendo del porcentaje de influencia dentro de la muestra del IPC. Entre los sectores fuertes se ubicó a comunicaciones y transportes, comercio, construcción e industria extractiva; mientras que en los débiles a transformación, servicios y varios. El modelo de regresión utilizado fue el propuesto por Marshall y Walker (2002, con el que se buscó comprobar que los sectores fuertes ajustaban más rápidamente sus precios que los sectores débiles con respecto a la rentabilidad del mercado. Se encontró que existe evidencia de que los retornos de los sectores fuertes se adelantan a los retornos de los sectores débiles, principalmente porque los primeros ajustan más rápidamente sus precios a los movimientos del mercado.

  16. A novel respiratory syncytial virus (RSV F subunit vaccine adjuvanted with GLA-SE elicits robust protective TH1-type humoral and cellular immunity in rodent models.

    Directory of Open Access Journals (Sweden)

    Stacie L Lambert

    Full Text Available Illness associated with Respiratory Syncytial Virus (RSV remains an unmet medical need in both full-term infants and older adults. The fusion glycoprotein (F of RSV, which plays a key role in RSV infection and is a target of neutralizing antibodies, is an attractive vaccine target for inducing RSV-specific immunity.BALB/c mice and cotton rats, two well-characterized rodent models of RSV infection, were used to evaluate the immunogenicity of intramuscularly administered RSV vaccine candidates consisting of purified soluble F (sF protein formulated with TLR4 agonist glucopyranosyl lipid A (GLA, stable emulsion (SE, GLA-SE, or alum adjuvants. Protection from RSV challenge, serum RSV neutralizing responses, and anti-F IgG responses were induced by all of the tested adjuvanted RSV sF vaccine formulations. However, only RSV sF + GLA-SE induced robust F-specific TH1-biased humoral and cellular responses. In mice, these F-specific cellular responses include both CD4 and CD8 T cells, with F-specific polyfunctional CD8 T cells that traffic to the mouse lung following RSV challenge. This RSV sF + GLA-SE vaccine formulation can also induce robust RSV neutralizing titers and prime IFNγ-producing T cell responses in Sprague Dawley rats.These studies indicate that a protein subunit vaccine consisting of RSV sF + GLA-SE can induce robust neutralizing antibody and T cell responses to RSV, enhancing viral clearance via a TH1 immune-mediated mechanism. This vaccine may benefit older populations at risk for RSV disease.

  17. Herpes Simplex Virus Type 1 (HSV-1)-Induced Apoptosis in Human Dendritic Cells as a Result of Downregulation of Cellular FLICE-Inhibitory Protein and Reduced Expression of HSV-1 Antiapoptotic Latency-Associated Transcript Sequences▿

    OpenAIRE

    Kather, Angela; Raftery, Martin J.; Devi-Rao, Gayathri; Lippmann, Juliane; Giese, Thomas; Sandri-Goldin, Rozanne M.; Schönrich, Günther

    2009-01-01

    Herpes simplex virus type 1 (HSV-1) is one of the most frequent and successful human pathogens. It targets immature dendritic cells (iDCs) to interfere with the antiviral immune response. The mechanisms underlying apoptosis of HSV-1-infected iDCs are not fully understood. Previously, we have shown that HSV-1-induced apoptosis of iDCs is associated with downregulation of the cellular FLICE-inhibitory protein (c-FLIP), a potent inhibitor of caspase-8-mediated apoptosis. In this study, we prove ...

  18. Cellular: Toward personal communications

    Science.gov (United States)

    Heffernan, Stuart

    1991-09-01

    The cellular industry is one of the fastest growing segment of the telecommunications industry. With an estimated penetration rate of 20 percent in the near future, cellular is becoming an ubiquitous telecommunications service in the U.S. In this paper we will examine the major advancements in the cellular industry: customer equipment, cellular networks, engineering tools, customer support, and nationwide seamless service.

  19. Acciones y problemas maternos para manejar el peso del hijo de acuerdo a la percepción materna del peso y edad del hijo

    Directory of Open Access Journals (Sweden)

    Yolanda Flores-Peña

    2014-04-01

    Full Text Available Introducción: Percibir de forma adecuada el peso del hijo puede influir para que la madre inicie cambios para prevenir y tratar los problemas de peso. Objetivos: 1 Evaluar si las acciones y problemas para manejar el peso del hijo son diferentes de acuerdo al estado nutricional y percepción del peso del hijo y 2 Evaluar si las acciones y problemas son diferentes de acuerdo a edad del hijo. Métodos: Participaron 2840 madres e hijos (2 a 17 años del Noreste de México. Las madres contestaron: a ¿Creo que mi hijo esta?, b ¿Qué ha hecho para manejar el peso de su hijo? y c ¿Cuáles son los problemas? Se midió peso y talla del hijo. Se construyeron tablas de contingencia y se aplicó la prueba de Chi-cuadrada. Resultados: Cuando las madres de hijos con sobrepeso-obesidad perciben de forma adecuada esta situación realizan acciones relacionadas con la dieta (62,0% y 65,6% (Χ² = 307,02, gl = 9, p < ,001 y reconocen problemas (26,2% y 43,7% (Χ² = 131,46, gl = 6,p < ,001. Las madres de hijos de 1º a 6º grado realizan acciones en mayor proporción 51,2% (Χ² = 44,78, gl = 9, p < ,001 y señalan más problemas 21,8% (Χ² = 61,74, gl = 6,p < ,001 Conclusiones: Cuando se perciben los problemas de peso del hijo, se realizan acciones y se reconocen problemas. Se recomiendan intervenciones para percibir de forma adecuada el sobrepeso-obesidad y porporcionar información de acciones relacionadas a la dieta.

  20. ¿Influyen las Normas Internacionales de Información Financiera en el riesgo de las acciones?

    OpenAIRE

    Gokhan Boz; Carlota Menéndez Plans; Neus Orgaz Guerrero; Diego Prior Jiménez

    2015-01-01

    El objetivo del presente trabajo de investigación es doble: estudiar qué información explica el riesgo de las acciones y analizar si la implantación de las Normas Internacionales de Información Financiera (NIIF), realizada en el mercado espanol ˜ en 2005, influye en dicha información. Conocer qué información contable y/o macroeconómica explica el riesgo de una acción es una información útil para el empresario y para el profesional de la gestión, pues nos indica qué variables observar para est...

  1. Relación entre teoría de la justicia y acciones no-violentas

    Directory of Open Access Journals (Sweden)

    María del Rosario Guerra González

    2013-12-01

    Full Text Available Este texto tiene tres partes: en primer término se caracterizan diversas formas de acciones no-violentas, en segundo término se presenta la justificación de la desobediencia civil dentro de la teoría de Rawls y en tercera parte se amplía el problema del derecho interno de los Estados al sistema internacional. Por motivos de justicia cada día son más las situaciones que han provocado desobediencia civil. Primero fue la defensa de derechos civiles y políticos quien causó la resistencia, luego las motivaciones han cambiado. El tema está abierto.

  2. Ventajas y desventajas de la Sociedad por Acciones Simplificada para la empresa familiar en Colombia. Estudio exploratorio

    Directory of Open Access Journals (Sweden)

    José Bernardo Betancourt Ramírez

    2013-01-01

    Full Text Available La Sociedad por Acciones Simplificada (SAS representa la evolución más significativa del sistema societario colombiano en las últimas 4 décadas. Desde su aparición representa cerca del 90% del total de sociedades matriculadas en el país. Este estudio exploratorio descriptivo busca determinar si los empresarios conocen las ventajas y desventajas de las SAS, y si las tienen en cuenta en la toma de decisiones. Se aplicó un cuestionario estructurado a 57 empresas, de las cuales el 84% eran familia- res. Se observó un amplio desconocimiento de algunas ventajas y desventajas de la SAS que implica el desaprovechamiento de sus bondades y, en casos puntuales, riesgos para las empresas familiares.

  3. FONDOS DE INVERSIÓN EN ACCIONES EN BRASIL: MÉTRICAS PARA EVALUACIÓN DEL RENDIMIENTO

    Directory of Open Access Journals (Sweden)

    Bolivar Godinho de Oliveira Filho

    2015-08-01

    Full Text Available El objetivo de este trabajo es calcular los índices de rendimiento de los fondos de acciones en el mercado brasileño y determinar cuál es la probabilidad de un fondo, que está presentando buen rendimiento en relación a sus pares, de conseguir mantener esa posición en el período siguiente. Como objetivo específico, se buscó investigar cuáles índices de rendimiento están más asociados a la expectativa de buena performance. La principal contribución de este trabajo consiste en desarrollar un modelo para análisis de fondos de inversión en acciones en el mercado brasileño que pudiese ser utilizado por los inversores para seleccionar los mejores fondos para aplicación de sus recursos. Se buscó analizar cuales métricas de rendimiento son mejores para discriminar los fondos con buen rendimiento, a través de la técnica estadística de regresión logística binaria, cuya respuesta permite establecer la probabilidad de ocurrencia de fondos con rendimiento superior y la importancia de las variables para esa ocurrencia. El abordaje es innovador. Los resultados del trabajo mostraron que el modelo computó un índice de aciertos de 81% en su matriz de clasificación y definió las variables con importancia estadística que deben ser consideradas en la elección de los fondos, por el orden de importancia: tasa de administración, índice de Treynor, índice de Sharpe generalizado, índice de Modigliani y tasa de performance.

  4. Type I and III IFNs Produced by Plasmacytoid Dendritic Cells in Response to a Member of the Flaviviridae Suppress Cellular Immune Responses.

    Science.gov (United States)

    Reid, Elizabeth; Juleff, Nicholas; Windsor, Miriam; Gubbins, Simon; Roberts, Lisa; Morgan, Sophie; Meyers, Gregor; Perez-Martin, Eva; Tchilian, Elma; Charleston, Bryan; Seago, Julian

    2016-05-15

    The pestivirus noncytopathic bovine viral diarrhea virus (BVDV) can suppress IFN production in the majority of cell types in vitro. However, IFN is detectable in serum during acute infection in vivo for ∼5-7 d, which correlates with a period of leucopoenia and immunosuppression. In this study, we demonstrate that a highly enriched population of bovine plasmacytoid dendritic cells (DCs) produced IFN in response to BVDV in vitro. We further show that the majority of the IFN produced in response to infection both in vitro and in vivo is type III IFN and acid labile. Further, we show IL-28B (IFN-λ3) mRNA is induced in this cell population in vitro. Supernatant from plasmacytoid DCs harvested postinfection with BVDV or recombinant bovine IFN-α or human IL-28B significantly reduced CD4(+) T cell proliferation induced by tubercle bacillus Ag 85-stimulated monocyte-derived DCs. Furthermore, these IFNs induced IFN-stimulated gene expression predominantly in monocyte-derived DCs. IFN-treated immature DCs derived from murine bone marrow also had a reduced capacity to stimulate T cell proliferative responses to tubercle bacillus Ag 85. Immature DCs derived from either source had a reduced capacity for Ag uptake following IFN treatment that is dose dependent. Immunosuppression is a feature of a number of pestivirus infections; our studies suggest type III IFN production plays a key role in the pathogenesis of this family of viruses. Overall, in a natural host, we have demonstrated a link between the induction of type I and III IFN after acute viral infection and transient immunosuppression. PMID:27053760

  5. Revistas científicas de las universidades españolas: acciones básicas para aumentar su difusión e impacto

    Directory of Open Access Journals (Sweden)

    Abadal, Ernest

    2008-06-01

    Full Text Available A description of the current situation of scholarly journals from Spanish universities with the aim of highlighting certain actions to improve their dissemination. First, quantitative data is offered regarding the university scientific journals that originate from journal directories (essentially, Cindoc, Ulrich’s and Latindex. It then sets out a set of seven basic lines of action to improve their dissemination and impact: content digitalisation, inclusion on portals, free access dissemination, creation of multilingual versions, communication of new features, audience measuring and inclusion in databases. Finally, reference is made to the main agents that may carry out this type of action

    Se describe la situación actual de las revistas científicas de las universidades españolas con el propósito de señalar algunas acciones para mejorar su difusión. En primer lugar, se ofrecen diversos datos cuantitativos sobre las revistas científicas universitarias que proceden de directorios de revistas (CINDOC, Ulrich’s y Latindex, fundamentalmente. A continuación, se señalan un conjunto de siete líneas básicas de actuación para mejorar su difusión e impacto: digitalización de contenidos, inclusión en portales, difusión en libre acceso, elaboración de versiones multilingües, comunicación de novedades, medición de la audiencia, e inclusión en bases de datos. Finalmente, se hace referencia a los principales agentes que pueden llevar a cabo este tipo de actuaciones.

  6. Human stefin B normal and patho-physiological role: molecular and cellular aspects of amyloid-type aggregation of certain EPM1 mutants.

    Directory of Open Access Journals (Sweden)

    Mira ePolajnar

    2012-08-01

    Full Text Available Epilepsies are characterised by abnormal electrophysiological activity of the brain. Among various types of inherited epilepsies different epilepsy syndromes, among them progressive myoclonus epilepsies with features of ataxia and neurodegeneration, are counted. The progressive myoclonus epilepsy of type 1 (EPM1, also known as Unverricht-Lundborg disease presents with features of cerebellar atrophy and increased oxidative stress. It has been found that EPM1 is caused by mutations in human cystatin B gene (human stefin B. We first describe the role of protein aggregation in other neurodegenerative conditions. Protein aggregates appear intraneurally but are also excreted, such as is the case with senile plaques of amyloid- β (Aβ that accumulate in the brain parenchyma and vessel walls. A common characteristic of such diseases is the change of the protein conformation towards β secondary structure that accounts for the strong tendency of such proteins to aggregate and form amyloid fibrils. Second, we describe the patho-physiology of EPM1 and the normal and aberrant roles of stefin B in a mouse model of the disease. Furthermore, we discuss how the increased protein aggregation observed with some of the mutants of human stefin B may relate to the neurodegeneration that occurs in rare EPM1 patients. Our hypothesis (Ceru et al., 2005 states that some of the EPM1 mutants of human stefin B may undergo aggregation in neural cells, thus gaining additional toxic function (apart from loss of normal function. Our in vitro experiments thus far have confirmed that 4 mutants undergo increased aggregation relative to the wild-type protein. It has been shown that the R68X mutant forms amyloid-fibrils very rapidly, even at neutral pH and forms perinuclear inclusions, whereas the G4R mutant exhibits a prolonged lag phase, during which the toxic prefibrillar aggregates accumulate and are scattered more diffusely over the cytoplasm. Initial experiments on the G50E

  7. Protein Turnover and Cellular Stress in Mildly and Severely Affected Muscles from Patients with Limb Girdle Muscular Dystrophy Type 2I

    DEFF Research Database (Denmark)

    Hauerslev, Simon; Sveen, Marie-Louise; Vissing, John;

    2013-01-01

    Patients with Limb girdle muscular dystrophy type 2I (LGMD2I) are characterized by progressive muscle weakness and wasting primarily in the proximal muscles, while distal muscles often are spared. Our aim was to investigate if wasting could be caused by impaired regeneration in the proximal...... stress were highly increased in severely affected muscles compared to mildly affected muscles. Our results indicate that alterations in the protein turnover and myostatin levels could progressively impair the muscle mass maintenance and/or regeneration resulting in gradual muscular atrophy....

  8. PERFIL INTRAEMPRENDEDOR Y ACCIONES DE LOS GESTORES DE LOS CURSOS DE CIENCIAS CONTABLES DE LA REGIÓN OESTE DE PARANÁ

    Directory of Open Access Journals (Sweden)

    Silvana Anita Walter

    2012-03-01

    Full Text Available Este artículo tiene como objetivo identificar y analizar las acciones, el perfil y las características intraemprendedoras de los gestores de cursos de bachillerato en Ciencias Contables del Oeste de Paraná. De esa manera, cipales acciones de los gestores de cursos de Ciencias Contables de la región Oeste de Paraná? Se ha utilizado como método la investigación descriptiva del tipo survey y, para la recolección de los datos, un cuestionario de Mastella (2005 y de Dornelas (2003, adaptado por Walter et. al. (2007. Como principales resultados, se há constatado que los gestores son hombres, teniendo la mayoría de ellos más de 50 años de edad, título de especialista o maestría y el principal vínculo con la institución en la cual actúan. Se ha notado que las acciones más desarrolladas por los gestores se presentan destinadas a las acciones internas, hecho que debería ser objeto de reflexión, una vez que las acciones consideradas más importantes por los gestores se llevan a cabo no muy a menudo. De esa manera, con poco tiempo de dedicación a la gestión, con discrepancias entre las acciones deseadas y las efectivamente llevadas a cabo y el demasiado enfoque interno de sus acciones son aspectos que los gestores investigados necesitan mejorar. Todos los investigados presentan características intraemprendedoras. Se nota la necesidad de una mayor claridad en el diálogo entre el personal superior y los gestores para una mayor alineación en cuanto al desempeño actual de los gestores. Se nota asimismo que cuestiones como la conciliación entre la carga horaria enseñanza/gestión todavía necesita perfeccionarse.

  9. Cellular identity of an 18S rRNA gene sequence clade within the class Kinetoplastea: the novel genus Actuariola gen. nov. (Neobodonida) with description of the type species Actuariola framvarensis sp. nov.

    Science.gov (United States)

    Stoeck, Thorsten; Schwarz, M V Julian; Boenigk, Jens; Schweikert, Michael; von der Heyden, Sophie; Behnke, Anke

    2005-11-01

    Environmental molecular surveys of microbial diversity have uncovered a vast number of novel taxonomic units in the eukaryotic tree of life that are exclusively known by their small-subunit (SSU) rRNA gene signatures. In this study, we reveal the cellular and taxonomic identity of a novel eukaryote SSU rRNA gene sequence clade within the Kinetoplastea. Kinetoplastea are ubiquitously distributed flagellated protists of high ecological and medical importance. We isolated an organism from the oxic-anoxic interface of the anoxic Framvaren Fjord (Norway), which branches within an unidentified kinetoplastean sequence clade. Ultrastructural studies revealed a typical cellular organization that characterized the flagellated isolate as a member of the order Neobodonida Vickerman 2004, which contains five genera. The isolate differed in several distinctive characters from Dimastigella, Cruzella, Rhynchobodo and Rhynchomonas. The arrangement of the microtubular rod that supports the apical cytostome and the cytopharynx differed from the diagnosis of the fifth described genus (Neobodo Vickerman 2004) within the order Neobodonida. On the basis of both molecular and microscopical data, a novel genus within the order Neobodonida, Actuariola gen. nov., is proposed. Here, we characterize its type species, Actuariola framvarensis sp. nov., and provide an in situ tool to access the organism in nature and study its ecology.

  10. trans activation by the full-length E2 proteins of human papillomavirus type 16 and bovine papillomavirus type 1 in vitro and in vivo: cooperation with activation domains of cellular transcription factors.

    OpenAIRE

    Ushikai, M; Lace, M J; Yamakawa, Y.; Kono, M; Anson, J; Ishiji, T; Parkkinen, S; Wicker, N.; Valentine, M E; Davidson, I

    1994-01-01

    Papillomaviral E2 genes encode proteins that regulate viral transcription. While the full-length bovine papillomavirus type 1 (BPV-1) E2 peptide is a strong trans activator, the homologous full-length E2 product of human papillomavirus type 16 (HPV-16) appeared to vary in function in previous studies. Here we show that when expressed from comparable constructs, the full-length E2 products of HPV-16 and BPV-1 trans activate a simple E2- and Sp1-dependent promoter up to approximately 100-fold i...

  11. EL COMPORTAMIENTO DEL PRECIO DE LAS ACCIONES Y RECESIÓN ECONÓMICA. EEUU Y MÉXICO

    Directory of Open Access Journals (Sweden)

    Jorge Vázquez Sánchez

    2010-01-01

    Full Text Available En este artículo se hace un estudio de las correspondientes crisis en los Estados Unidos y en México, distinguiendo entre su aspecto financiero y el económico. Se enfocan las crisis financieras como ciclos naturales que pueden terminar en recesiones económicas, más o menos profundas, en cuyo caso estarían desempeñando una función profiláctica para eliminar el exceso de inversión generado durante la etapa de expansión. La intervención del Banco Central resulta muy importante para mitigar las restricciones de liquidez y de esa manera atemperar la depreciación de activos y, en su caso, la profundidad de la recesión. El estudio se sustenta en el modelo de la crisis financiera propuesto por Minsky y Kindleberger, así como en el análisis técnico del comportamiento del precio de las acciones.

  12. Actions in the electrical sector to propitiate the environmental protection; Acciones en el sector electrico para propiciar la proteccion ambiental

    Energy Technology Data Exchange (ETDEWEB)

    Trevino Gaspari, Mateo [Fideicomiso de Ahorro de Energia (FIDE) (Mexico)

    1997-07-01

    The saving of electrical energy is one of the most efficient means to propitiate the environmental protection in the surroundings of the facilities used in the production of electrical energy. The emission of polluting agents associated with the electric power generation is analyzed, among them, CO{sub 2}, SO{sub 2} and NO{sub 2}, along with the particulate emission of ash or soot. In addition, an analysis is made of the contribution of the electrical energy saving to the environmental protection and the actions that the Comision Federal de Electricidad of Mexico will undertake on the electric energy saving for the 1995 to the 2000 period. [Spanish] El ahorro de energia electrica es uno de los medios mas eficientes para propiciar la proteccion ambiental en el entorno de las instalaciones usadas en la produccion de energia electrica. Se analiza la emision de contaminantes asociados con la generacion electrica, entre ellos, el CO{sub 2}, el SO{sub 2} y NO{sub 2}, junto con la emision de particulas de ceniza o de hollin. Ademas se analiza la contribucion del ahorro de energia electrica a la proteccion ambiental y las acciones que emprendera la Comision Federal de Electricidad de Mexico sobre ahorro de energia electrica para el periodo 1995 al 2000.

  13. Acciones gubernamentales frente a los desastres provocados por fenómenos hidrometeorológicos en México

    Directory of Open Access Journals (Sweden)

    Gabriel Angelotti Pasteur

    2015-02-01

    Full Text Available Las acciones realizadas tras los desastres constituyen una oportunidad para el cambio social, en específico, para construir sociedades resilientes. Sin embargo, cuando en los procesos de reconstrucción se persiguen intereses exógenos (económicos, sociales o políticos y, preferentemente, ajenos a las necesidades de la sociedad afectada, se incrementan las condiciones de riesgo y la vulnerabilidad ante futuras amenazas. En el presente trabajo se analiza lo ocurrido en Yucatán (México cuando, tras el paso del huracán Isidoro (2002, se implementó un programa de reconstrucción con el fin de suplir aquellas viviendas que habían sido dañadas y destruidas por ese fenómeno hidrometeorológico. Mediante el mismo se esperaba dotar a las familias damnificadas de una vivienda digna, resistente, moderna y, de este modo, contribuir a forjar una sociedad más segura. Sin embargo, los resultados alcanzados ponen en duda esta meta del gobierno.

  14. ESTRATEGIAS DE ACCION E INCIDENCIA EN LAS POLITICAS PUBLICAS DE LAS COOPERATIVAS DE TERCER GRADO EN ARGENTINA

    Directory of Open Access Journals (Sweden)

    María Cristina Acosta

    2011-01-01

    Full Text Available Este trabajo indaga sobre la incidencia de las entidades representativas del cooperativismo argentino en las políticas públicas contemporáneas. Se presenta un estudio de caso de las dos más importantes cooperativas de tercer grado del país, se analizan sus metas y estrategias en las interacciones con las dependencias estatales. Los resultados de las acciones de los actores definen metas y estrategias que pueden ser convergentes y/o divergentes. Se concluye que los vínculos que han construido las confederaciones seleccionadas con el Estado presentan características de complementación y cooperación en términos generales. Asimismo, la facultad de las organizaciones estudiadas para incidir en la definición de las políticas públicas resulta no solamente de los recursos propios, sino, principalmente, de las oportunidades políticas y la capacidad de establecer alianzas inter-actorales mediante estrategias colectivas.

  15. ACCIONES PEDAGÓGICAS PARA FAVORECER LA COMUNICACIÓN INTERALUMNO EN EL TRANSCURSO DE LA ACTIVIDAD DOCENTE

    Directory of Open Access Journals (Sweden)

    Greccy Castro Miranda

    2014-06-01

    Full Text Available La comunicación pedagógica es un proceso básico de profesionalidad en los educadores, por la significación de esta para el efectivo desarrollo del proceso de enseñanza aprendizaje. En el artículo [3] se propone un conjunto de talleres que favorecen la comunicación interalumno, se desarrolló por la necesidad de atender las dificultades manifestadas en el proceso de la comunicación en la clase, en particular, por los problemas que presentan los estudiantes en su comunicación, lo que atenta contra el protagonismo de estos en el proceso de su educación como futuros profesores y en su propia vida personal. El objetivo trazado fue aplicar acciones pedagógicas en forma de talleres que favorezcan la comunicación interalumnos. Se introdujeron talleres práctico-vivenciales para evaluar el desarrollo de la comunicación pedagógica como elemento básico para el desarrollo de habilidades comunicativas y como vía que contribuye a la competencia comunicativa del futuro profesor.

  16. Cambio climático y salud pública: acciones desde la institucionalidad en el escenario sociocultural actual

    Directory of Open Access Journals (Sweden)

    Lizet Helena Veliz Rojas

    2013-12-01

    Full Text Available Objetivo: Analizar el vínculo entre el cambio climático y la salud de las personas, reflexionando en torno a los desafíos que representa tanto para la atención primaria en salud pública cómo para los profesionales de la salud. Método: Este ensayo se organiza en tres momentos: En el primero, se describe brevemente la epistemología del medio ambiente como objeto de estudio. El segundo momento señala la influencia del desarrollo y crecimiento económico sobre los cambios ambientales, se reflexiona acerca de la vulnerabilidad de la población frente al cambio climático. Se analizan los efectos y consecuencias del cambio climático en la salud de las personas. Finalmente se exponen las acciones a nivel de la condicionante de la salud medio ambiental propuesta para la salud pública. Conclusión: El empoderamiento de las personas por parte de los profesionales de la salud resulta significativo al momento de movilizar capital social para el desarrollo de programas y políticas públicas en salud y medio ambiente.

  17. CELLULAR INTERACTIONS MEDIATED BY GLYCONECTIDS

    OpenAIRE

    Popescu, O.; Sumanovski, L. T.; I. Checiu; Elisabeta Popescu; G. N. Misevic

    1999-01-01

    Cellular interactions involve many types of cell surface molecules and operate via homophilic and/or heterophilic protein-protein and protein-carbohydrate binding. Our investigations in different model-systems (marine invertebrates and mammals) have provided direct evidence that a novel class of primordial proteoglycans, named by us gliconectins, can mediate cell adhesion via a new alternative molecular mechanism of polyvalent carbohydrate-carbohydrate binding. Biochemical characterization of...

  18. Reduction of porcine circovirus type 2 (PCV2 viremia by a reformulated inactivated chimeric PCV1-2 vaccine-induced humoral and cellular immunity after experimental PCV2 challenge

    Directory of Open Access Journals (Sweden)

    Seo Hwi

    2012-10-01

    Full Text Available Abstract Background The objective of the present study was to elucidate the humoral and cellular immune response mechanisms by which a reformulated inactivated chimeric PCV1-2 vaccine reduces the PCV2 viremia. Forty PCV2 seronegative 3-week-old pigs were randomly divided into the following four groups: vaccinated challenged (T01, vaccinated non-challenged (T02, non-vaccinated challenged (T03, and non-vaccinated non-challenged (T04 animals. The pigs in groups T01 and T02 were immunized with a reformulated inactivated chimeric PCV1-2 vaccine (Fostera™ PCV; Pfizer Animal Health administered as a 2.0 ml dose at 21 days of age. At 35 days of age (0 days post-challenge, the pigs in groups T01 and T03 were inoculated intranasally with 2 ml each of PCV2b. Results A reduction of PCV2 viremia coincided with the appearance of both PCV2-specific neutralizing antibodies (NA and interferon-γ-secreting cells (IFN-γ-SCs in the vaccinated animals. However, the presence of anti-PCV2 IgG antibodies did not correlate with the reduction of PCV2 viremia. Lymphocyte subset analysis indicated that the numbers of CD3+ and CD4+ cells increased in vaccinated animals but the numbers of CD4+ cells decreased transiently in non-vaccinated animals. The observation of a delayed type hypersensitivity response in only the vaccinated animals also supports a CD4+ cell-associated protective cellular immune response induced by the reformulated inactivated chimeric PCV1-2 vaccine. Conclusions The induction of PCV2-specific NA and IFN-γ-SCs, and CD4+ cells by the reformulated inactivated chimeric PCV1-2 vaccine is the important protective immune response leading to reduction of the PCV2 viremia and control of the PCV2 infection. To our knowledge this is the first demonstration of protective humoral and cellular immunity induced by the reformulated inactivated chimeric PCV1-2 vaccine and its effect on reduction of PCV2 viremia by vaccination.

  19. Una aproximación a la predicción del valor de acciones en la bolsa de valores aplicando técnicas de Data Mining

    OpenAIRE

    Espinosa Muñoz, Javier Isaac

    2015-01-01

    La predicción del valor de las acciones en la bolsa de valores ha sido un tema importante en el campo de inversiones, que por varios años ha atraído tanto a académicos como a inversionistas. Esto supone que la información disponible en el pasado de la compañía que cotiza en bolsa tiene alguna implicación en el futuro del valor de la misma. Este trabajo está enfocado en ayudar a un persona u organismo que decida invertir en la bolsa de valores a través de gestión de compra o venta de acciones ...

  20. Acciones de resiliencia frente a las políticas habitacionales en la ciudad de Buenos Aires: las organizaciones sociales, las instituciones y los desalojos masivos

    Directory of Open Access Journals (Sweden)

    Sandra Inu00E9s Su00E1nchez

    2013-01-01

    Full Text Available La problemática habitacional actual de la ciudad de Buenos Aires gira en torno a dos fenómenos: la precariedad y los desalojos, en un contexto que se concibe de emergencia habitacional. Como reacción a esta situación, algunos organismos institucionales y ciertas organizaciones sociales con anclaje territorial en la zona sur de la ciudad comenzaron a llevar adelante acciones de resiliencia frente a los desalojos masivos que, como consecuencia de la presión inmobiliaria, afectaban a los hoteles, pensiones, inquilinatos y casas tomadas de esta zona de la ciudad. Se analizarán las acciones de resiliencia desplegadas por ellos en sus dimensiones individuales y colectivas y su relación con las políticas habitacionales.

  1. Acciones de resiliencia frente a las políticas habitacionales en la ciudad de Buenos Aires: las organizaciones sociales, las instituciones y los desalojos masivos

    Directory of Open Access Journals (Sweden)

    Sandra Inés Sánchez

    2013-06-01

    Full Text Available La problemática habitacional actual de la ciudad de Buenos Aires gira en torno a dos fenómenos: la precariedad y los desalojos, en un contexto que se concibe de emergencia habitacional. Como reacción a esta situación, algunos organismos institucionales y ciertas organizaciones sociales con anclaje territorial en la zona sur de la ciudad comenzaron a llevar adelante acciones de resiliencia frente a los desalojos masivos que, como consecuencia de la presión inmobiliaria, afectaban a los hoteles, pensiones, inquilinatos y casas tomadas de esta zona de la ciudad. Se analizarán las acciones de resiliencia desplegadas por ellos en sus dimensiones individuales y colectivas y su relación con las políticas habitacionales.

  2. Una aplicación de algoritmos genéticos a la regla de filtro en la transacción de acciones

    OpenAIRE

    Hernández Chanto, Allan

    2009-01-01

    En el mercado de acciones, una rgla de filtro se define como una herramienta técnica que le permite a los agentes involucados definir una estrategia sobre cuándo comprar o vender sus acciones, a partir de la determinación de ciertos parámetros. El asunto crucial radica en cómo seleccionar la combinación óptima de los parámetros dentro de un espacio de búsqueda suficientemente grande. En este artículo se aplica la técnica heurística de algoritmos genéticos, paa encontrar la combinación cuasi-ó...

  3. Optimization of Inter Cellular Movement of Parts in Cellular Manufacturing System Using Genetic Algorithm

    OpenAIRE

    Siva Prasad Darla; C.D. Naiju; Polu Vidya Sagar; B. Venkat Likhit

    2014-01-01

    In the modern manufacturing environment, Cellular Manufacturing Systems (CMS) have gained greater importance in job shop or batch-type production to gain economic advantage similar to those of mass production. Successful implementation of CMS highly depends on the determination of part families; machine cells and minimizing inter cellular movement. This study considers machine component grouping problems namely inter-cellular movement and cell load variation by developing a mathematical model...

  4. Metodología para la toma de decisiones de inversión en portafolio de acciones utilizando la técnica multicriterio AHP

    Directory of Open Access Journals (Sweden)

    John Willmer Escobar

    2015-01-01

    Full Text Available Este artículo presenta una metodología para la toma de decisiones en el mercado accionario colombiano utilizando la técnica multicriterio Analytic Hierarchy Process (AHP. La problemática está relacionada con el proceso de inversión en un mercado bursátil considerando criterios de riesgo y rentabilidad. La metodología propuesta incluye la integración de las técnicas tradicionales de decisión de inversión en portafolio de acciones junto con la técnica multicriterio AHP que permite evaluar un número finito de alternativas de manera jerárquica bajo criterios cualitativos y cuantitativos. La metodología ha sido probada en la solución del problema de selección de portafolio de acciones de alta y media bursatilidad que cotizaron en el mercado colombiano durante el periodo de diciembre de 2007 a abril de 2010. Los resultados computacionales muestran la importancia y la eficiencia de la integración exitosa de los criterios tradicionales de inversión en portafolio de acciones junto con la metodología AHP para encontrar un balance apropiado entre rentabilidad y riesgo en el proceso de toma de decisiones de inversión en acciones en el mercado bursátil colombiano. Derechos reservados © 2015 Universidad Nacional Autónoma de México, Facultad de Contaduría y Administración. Este es un artículo de acceso abierto distribuido bajo los términos de la Licencia Creative Commons CC BY-NC-ND 4.0.

  5. Acciones colectivas y políticas públicas territoriales: el caso de las víctimas en Medellín

    Directory of Open Access Journals (Sweden)

    James Gilberto Granada Vahos

    2014-10-01

    Full Text Available En este artículo, que presenta resultados del proyecto “Políticas públicas territoriales en Medellín, actores, recursos y oportunidades. Las políticas públicas de desplazamiento forzado, y de las diversidades sexuales y de género”, se propone realizar una lectura de las acciones colectivas de la población víctima en Medellín en relación con las políticas públicas territoriales de atención a víctimas, de esta manera se continúa con una agenda investigativa sobre esos dos temas y sus relaciones: las acciones colectivas de las víctimas del conflicto armado y las políticas públicas territoriales en Medellín y Antioquia. En el texto, se desarrolla un contexto general de la política pública de víctimas y algunos de sus antecedentes en la política de desplazamiento forzado; así mismo, se hace una aproximación a las acciones colectivas de las víctimas en y de la política pública territorial, se aborda brevemente lo que pasa de las políticas nacionales a los territorios, la relación con estos expresada en la descentralización y en los enfoques territoriales; y se finaliza con algunas consideraciones sobre el vínculo, en Medellín, de las acciones colectivas y las políticas públicas de las víctimas del conflicto armado.

  6. Modelos de representación de características para la clasificación de acciones humanas en video: estado del arte

    Directory of Open Access Journals (Sweden)

    Ruber Hernández García

    2014-10-01

    Full Text Available La clasificación de acciones humanas en video es un área del conocimiento muy activa en la comunidad científica de la visión por computador. El objetivo de este campo de investigación es clasificar automáticamente acciones humanas a partir de los fotogramas que componen una secuencia de video, utilizando para ello técnicas de reconocimiento de patrones. El rendimiento de los métodos de reconocimiento de patrones depende en gran medida de la representación de los datos utilizada. Por esta razón, se centra la atención en el análisis del estado del arte referente a los modelos de representación de la información visual para la clasificación de acciones humanas en videos. El presente trabajo tiene como objetivo examinar desde un enfoque crítico las diferentes aproximaciones reportadas, así como los referentes teóricos de la temática tratada. A partir del estudio realizado se logró concluir que la aplicación de técnicas de selección de características, el uso de modelos relacionales y la obtención de una representación basada en n-gramas visuales, figuran como alternativas interesantes a incorporar como parte de los modelos de representación de características para la clasificación de acciones humanas.

  7. Modelling cellular behaviour

    Science.gov (United States)

    Endy, Drew; Brent, Roger

    2001-01-01

    Representations of cellular processes that can be used to compute their future behaviour would be of general scientific and practical value. But past attempts to construct such representations have been disappointing. This is now changing. Increases in biological understanding combined with advances in computational methods and in computer power make it possible to foresee construction of useful and predictive simulations of cellular processes.

  8. ACCIONES DE LUCHA CONTRA LA DESERTIFICACIÓN EN AMBIENTES SEMIÁRIDOS EN EL NOROESTE DE TAMAULIPAS, MÉXICO

    Directory of Open Access Journals (Sweden)

    Elizabeth Del C. Andrade Limas

    2009-01-01

    Full Text Available En la Cuenca de Burgos, Tamaulipas (México, se realizaron acciones de lucha contra la desertifi cación para ayudar a la conservación de suelo y agua. El trabajo se efectuó en dos etapas 2005-2007 y 2008-2009 en lugares con problemas de erosión por pérdida de cobertura vegetal y deforestación causada por la agricultura y las actividades industriales. El objetivo fue estabilizar el ecosistema para conservar y manejar los recursos naturales, incrementar la producción agropecuaria, mejorar la condición familiar y elevar el nivel de vida de los pobladores rurales. Se identifi caron y ubicaron áreas y parcelas de trabajo. Se realizaron y evaluaron las siguientes acciones con el objetivo de obtener indicadores de sostenibilidad: (1 111,8 km de Cercos Vivos, (2 53,5 has con Bancos de Proteína, (3 825 has con introducción de Especies Forrajeras, (4 20 has de Estanques y abrevaderos, (5 3.579 has de subsoleo (6 10 has de sistemas silvopatoriles con Prosopis glandulosa (Mezquite, y pasto, y (7 realización de 5 Cursos de Capacitación para los agricultores. Todas estas acciones, sin duda, han sido muy benefi ciosas, aunque es necesaria una mayor información a los agricultores.

  9. Reversible quantum cellular automata

    CERN Document Server

    Schumacher, B

    2004-01-01

    We define quantum cellular automata as infinite quantum lattice systems with discrete time dynamics, such that the time step commutes with lattice translations and has strictly finite propagation speed. In contrast to earlier definitions this allows us to give an explicit characterization of all local rules generating such automata. The same local rules also generate the global time step for automata with periodic boundary conditions. Our main structure theorem asserts that any quantum cellular automaton is structurally reversible, i.e., that it can be obtained by applying two blockwise unitary operations in a generalized Margolus partitioning scheme. This implies that, in contrast to the classical case, the inverse of a nearest neighbor quantum cellular automaton is again a nearest neighbor automaton. We present several construction methods for quantum cellular automata, based on unitaries commuting with their translates, on the quantization of (arbitrary) reversible classical cellular automata, on quantum c...

  10. Progress of cellular dedifferentiation research

    Institute of Scientific and Technical Information of China (English)

    LIU Hu-xian; HU Da-hai; JIA Chi-yu; FU Xiao-bing

    2006-01-01

    Differentiation, the stepwise specialization of cells, and transdifferentiation, the apparent switching of one cell type into another, capture much of the stem cell spotlight. But dedifferentiation, the developmental reversal of a cell before it reinvents itself, is an important process too. In multicellular organisms, cellular dedifferentiation is the major process underlying totipotency, regeneration and formation of new stem cell lineages. In humans,dedifferentiation is often associated with carcinogenesis.The study of cellular dedifferentiation in animals,particularly early events related to cell fate-switch and determination, is limited by the lack of a suitable,convenient experimental system. The classic example of dedifferentiation is limb and tail regeneration in urodele amphibians, such as salamanders. Recently, several investigators have shown that certain mammalian cell types can be induced to dedifferentiate to progenitor cells when stimulated with the appropriate signals or materials. These discoveries open the possibility that researchers might enhance the endogenous regenerative capacity of mammals by inducing cellular dedifferentiation in vivo.

  11. ¿Influyen las Normas Internacionales de Información Financiera en el riesgo de las acciones?

    Directory of Open Access Journals (Sweden)

    Gokhan Boz

    2015-01-01

    Full Text Available El objetivo del presente trabajo de investigación es doble: estudiar qué información explica el riesgo de las acciones y analizar si la implantación de las Normas Internacionales de Información Financiera (NIIF, realizada en el mercado espanol ˜ en 2005, influye en dicha información. Conocer qué información contable y/o macroeconómica explica el riesgo de una acción es una información útil para el empresario y para el profesional de la gestión, pues nos indica qué variables observar para estimar la evolución del riesgo y, en consecuencia, la evolución del coste de capital o tasa de actualización. La tasa de actualización es una variable muy importante en muchas decisiones financieras, y su valor, que depende entre otras variables del riesgo, tiene una gran influencia en la decisión. Por este motivo es importante que dicha tasa se determine de forma objetiva, algo especialmente difícil en las pequenas ˜ y medianas empresas (PYMES. Una gestión eficiente implica poder anticiparse a los efectosfuturos, actuar para capturar los efectos positivos y minimizar los efectos negativos. Si sabemos qué variables explican el riesgo, conoceremos qué indicadores observar para analizar su comportamiento y prever lo que vaya a suceder en el futuro sobre la tasa de actualización.

  12. Optimization of Inter Cellular Movement of Parts in Cellular Manufacturing System Using Genetic Algorithm

    Directory of Open Access Journals (Sweden)

    Siva Prasad Darla

    2014-01-01

    Full Text Available In the modern manufacturing environment, Cellular Manufacturing Systems (CMS have gained greater importance in job shop or batch-type production to gain economic advantage similar to those of mass production. Successful implementation of CMS highly depends on the determination of part families; machine cells and minimizing inter cellular movement. This study considers machine component grouping problems namely inter-cellular movement and cell load variation by developing a mathematical model and optimizing the solution using Genetic Algorithm to arrive at a cell formation to minimize the inter-cellular movement and cell load variation. The results are presented with a numerical example.

  13. Reversibly assembled cellular composite materials.

    Science.gov (United States)

    Cheung, Kenneth C; Gershenfeld, Neil

    2013-09-13

    We introduce composite materials made by reversibly assembling a three-dimensional lattice of mass-produced carbon fiber-reinforced polymer composite parts with integrated mechanical interlocking connections. The resulting cellular composite materials can respond as an elastic solid with an extremely large measured modulus for an ultralight material (12.3 megapascals at a density of 7.2 milligrams per cubic centimeter). These materials offer a hierarchical decomposition in modeling, with bulk properties that can be predicted from component measurements and deformation modes that can be determined by the placement of part types. Because site locations are locally constrained, structures can be produced in a relative assembly process that merges desirable features of fiber composites, cellular materials, and additive manufacturing.

  14. Heterogeneous cellular networks

    CERN Document Server

    Hu, Rose Qingyang

    2013-01-01

    A timely publication providing coverage of radio resource management, mobility management and standardization in heterogeneous cellular networks The topic of heterogeneous cellular networks has gained momentum in industry and the research community, attracting the attention of standardization bodies such as 3GPP LTE and IEEE 802.16j, whose objectives are looking into increasing the capacity and coverage of the cellular networks. This book focuses on recent progresses,  covering the related topics including scenarios of heterogeneous network deployment, interference management i

  15. Cellular chain formation in Escherichia coli biofilms

    DEFF Research Database (Denmark)

    Vejborg, Rebecca Munk; Klemm, Per

    2009-01-01

    In this study we report on a novel structural phenotype in Escherichia coli biofilms: cellular chain formation. Biofilm chaining in E. coli K-12 was found to occur primarily by clonal expansion, but was not due to filamentous growth. Rather, chain formation was the result of intercellular......; type I fimbriae expression significantly reduced cellular chain formation, presumably by steric hindrance. Cellular chain formation did not appear to be specific to E coli K-12. Although many urinary tract infection (UTI) isolates were found to form rather homogeneous, flat biofilms, three isolates...

  16. Cellular Restriction Factors of Feline Immunodeficiency Virus

    OpenAIRE

    Carsten Münk; Jörg Zielonka

    2011-01-01

    Lentiviruses are known for their narrow cell- and species-tropisms, which are determined by cellular proteins whose absence or presence either support viral replication (dependency factors, cofactors) or inhibit viral replication (restriction factors). Similar to Human immunodeficiency virus type 1 (HIV-1), the cat lentivirus Feline immunodeficiency virus (FIV) is sensitive to recently discovered cellular restriction factors from non-host species that are able to stop viruses from replicating...

  17. Nanostructured cellular networks.

    Science.gov (United States)

    Moriarty, P; Taylor, M D R; Brust, M

    2002-12-01

    Au nanocrystals spin-coated onto silicon from toluene form cellular networks. A quantitative statistical crystallography analysis shows that intercellular correlations drive the networks far from statistical equilibrium. Spin-coating from hexane does not produce cellular structure, yet a strong correlation is retained in the positions of nanocrystal aggregates. Mechanisms based on Marangoni convection alone cannot account for the variety of patterns observed, and we argue that spinodal decomposition plays an important role in foam formation.

  18. The insect cellular immune response

    Institute of Scientific and Technical Information of China (English)

    Michael R. Strand

    2008-01-01

    The innate immune system of insects is divided into humoral defenses that include the production of soluble effector molecules and cellular defenses like phagocytosis and encapsulation that are mediated by hemocytes. This review summarizes current understanding of the cellular immune response. Insects produce several terminally differentiated types of hemocytes that are distinguished by morphology, molecular and antigenic markers, and function. The differentiated hemocytes that circulate in larval or nymphal stage insects arise from two sources: progenitor cells produced during embryogenesis and mesodermally derived hematopoietic organs. Regulation of hematopoiesis and hemocyte differentiation also involves several different signaling pathways. Phagocytosis and encapsulation require that hemocytes first recognize a given target as foreign followed by activation of downstream signaling and effector responses. A number of humoral and cellular receptors have been identified that recognize different microbes and multicellular parasites. In turn, activation of these receptors stimulates a number of signaling pathways that regulate different hemocyte functions. Recent studies also identify hemocytes as important sources of a number of humoral effector molecules required for killing different foreign invaders.

  19. Evolución de las acciones educativas en el exterior (AEE de España en Francia

    Directory of Open Access Journals (Sweden)

    Mª Remedios González

    2013-01-01

    Full Text Available Este artículo analiza cómo se enfrentan los alumnos que terminan el Bachillerato a su futuro laboral y formativo a través de una encuesta realizadas en la primavera del 2011 a alumnos del distrito madrileño de la Universidad Complutense, entre los que se encuentran los del Liceo Español de París. La comparación entre los resultados de los alumnos del Liceo y los de sus compañeros parece indicar un cambio de modelo en los receptores de las acciones educativas españolas en el exterior. Por otro lado y desde hace años, la política común y los objetivos educativos europeos han estado marcados por la llamada «Estrategia de Lisboa» que ha apostado por empleos de calidad, basados en la formación, que llevarían a la innovación y al desarrollo tecnológico y esta política debería tener refl ejo en las expectativas de los alumnos. La situación de crisis económica que atraviesa Europa y en particular España, desde el 2008, introduce un nuevo escenario que puede influir en la toma de decisiones en este periodo tan significativo de final de la Enseñanza Secundaria que coincide con la incorporación de los alumnos a la ciudadanía plena y, en ocasiones, con su incorporación al mundo laboral.This article discusses how to confront students completing high school to their future employment and training through a survey conducted in the spring of 2011 with students in the Madrid’s district of Complutense University, among which are those of the Spanish Lyceum of Paris. The comparison between the results of the Lyceum students and their peers suggests a paradigm shift in the receptor of Spanish international education actions. On the other hand and for years now, the common policies and the European educational objectives have been marked by the «Lisbon Strategy » which has made a high bet on quality jobs, based on good education, which would lead to innovation and technological development and this policy should be reflected in

  20. Impacto de las acciones para la eliminación sostenible de la deficiencia de yodo en Cuba

    Directory of Open Access Journals (Sweden)

    C. Blanca Terry-Berro

    2014-03-01

    Full Text Available Objetivos: Evaluar la efectividad del “Programa de Eliminación Sostenible de Deficiencia de Yodo” mediante determinación de yoduria y prevalencia de bocio. Materiales y métodos. Se realizó un estudio transversal en el cual se incluyó a la población escolar cubana de 6 a 11 años que cursaba estudios en el periodo 2011-2012. Se empleó un muestreo estratificado polietápico. En el cual se determinaron los niveles de excreción de yodo en la orina (yoduria y la presencia de bocio. Resultados. La mediana de yoduria fue de 176,3 µg/L; el 7,6% de los escolares presentaron alguna deficiencia de yodo, solo el 2,2% estaba por debajo de 50 µg/L (deficiencia severa y el 15,3% tenía yodurias por encima de 300 µg/L. El porcentaje de escolares con nutrición óptima de yodo fue de 43,5%, esta categoría presentó una frecuencia significativamente mayor ( p=0,03 en el estrato montaña (52,5%. La prevalencia de bocio encontrada fue de 17,6%, que corresponde a una endemia ligera. Sin embargo, en el estrato montaña se encontró una prevalencia de 32,6 % (endemia severa. Conclusiones. El análisis integral del impacto de las acciones de control de la deficiencia de yodo evaluado a través de la excreción urinaria de yodo y la prevalencia de bocio refleja que ha dejado de ser un problema de salud poblacional en los estratos urbano y rural de Cuba, atribuible a un adecuado proceso de yodación de la sal. Sin embargo, aún se observa una alta prevalencia de bocio en la zona de montaña de Cuba

  1. Impacto de las acciones para la eliminación sostenible de la deficiencia de yodo en Cuba

    Directory of Open Access Journals (Sweden)

    C. Blanca Terry-Berro

    2014-01-01

    Full Text Available Objetivos: Evaluar la efectividad del “Programa de Eliminación Sostenible de Deficiencia de Yodo” mediante determinación de yoduria y prevalencia de bocio. Materiales y métodos. Se realizó un estudio transversal en el cual se incluyó a la población escolar cubana de 6 a 11 años que cursaba estudios en el periodo 2011-2012. Se empleó un muestreo estratificado polietápico. En el cual se determinaron los niveles de excreción de yodo en la orina (yoduria y la presencia de bocio. Resultados. La mediana de yoduria fue de 176,3 µg/L; el 7,6% de los escolares presentaron alguna deficiencia de yodo, solo el 2,2% estaba por debajo de 50 µg/L (deficiencia severa y el 15,3% tenía yodurias por encima de 300 µg/L. El porcentaje de escolares con nutrición óptima de yodo fue de 43,5%, esta categoría presentó una frecuencia significativamente mayor ( p=0,03 en el estrato montaña (52,5%. La prevalencia de bocio encontrada fue de 17,6%, que corresponde a una endemia ligera. Sin embargo, en el estrato montaña se encontró una prevalencia de 32,6 % (endemia severa. Conclusiones. El análisis integral del impacto de las acciones de control de la deficiencia de yodo evaluado a través de la excreción urinaria de yodo y la prevalencia de bocio refleja que ha dejado de ser un problema de salud poblacional en los estratos urbano y rural de Cuba, atribuible a un adecuado proceso de yodación de la sal. Sin embargo, aún se observa una alta prevalencia de bocio en la zona de montaña de Cuba

  2. Acciones afirmativas como mecanismo de inclusión social en la educación superior pública: el caso de la Región Caribe Colombiana

    Directory of Open Access Journals (Sweden)

    Jaime Alberto Morón Cárdenas

    2014-08-01

    Full Text Available Este trabajo se constituye en un primer insumo para la evaluación del impacto de la política de inclusión de las instituciones de educación superior de la región Caribe colombiana. El foco de atención se centra en dos aspectos: el análisis de las políticas universitarias implementadas por las siete IES públicas de la región para garantizar el acceso y permanencia de la población vulnerable, y el análisis de la dinámica presentada con la entrega de beneficios a los estudiantes matriculados entre 2005–2011 y su impacto en las tasas de deserción. Se utilizó información de acuerdos superiores y académicos, y resoluciones rectorales con las que se ofrecen beneficios a esta población, al igual que la base de datos del SPADIES. Se encontró que las acciones afirmativas implementadas por las IES se concentran en tres: académicas, financieras y de otro tipo, beneficiando al 52,7 % de los matriculados. Finalmente, para los estudiantes de estratos 1 y 2 las acciones afirmativas han buscado mejorar las condiciones de permanencia a través de apoyos de tipo financiero. Palabras clave: Acciones afirmativas; políticas de inclusión; población vulnerable; deserción.Affirmative action as social inclusion mechanism in public higher education: the case of the Colombian Caribbean RegionAbstract This article becomes the first product for the assessment about the impact of social inclusion policies on differents Higher Education Institutions (HEIs of the colombian Caribbean region. And it´s focused in two key aspects, the first one, it is the analysis of the impact of policies aimed to ensure the access and permanence of vulnerable population belong at the seven public university of the colombian Caribbean region; and another hand, the analysis about the supporting awarded to students matriculated among 2005 and 2011, and its impact on desertion rates. The information consulted was the senior and scademics agreement, and also rectoral

  3. Cellular Phone Towers, towers, various type of towers used for cell phone and communication purposes., Published in 2010, 1:2400 (1in=200ft) scale, Glynn County Board of Commissioners.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Cellular Phone Towers dataset, published at 1:2400 (1in=200ft) scale, was produced all or in part from Field Survey/GPS information as of 2010. It is described...

  4. Epigenetics and Cellular Metabolism

    Science.gov (United States)

    Xu, Wenyi; Wang, Fengzhong; Yu, Zhongsheng; Xin, Fengjiao

    2016-01-01

    Living eukaryotic systems evolve delicate cellular mechanisms for responding to various environmental signals. Among them, epigenetic machinery (DNA methylation, histone modifications, microRNAs, etc.) is the hub in transducing external stimuli into transcriptional response. Emerging evidence reveals the concept that epigenetic signatures are essential for the proper maintenance of cellular metabolism. On the other hand, the metabolite, a main environmental input, can also influence the processing of epigenetic memory. Here, we summarize the recent research progress in the epigenetic regulation of cellular metabolism and discuss how the dysfunction of epigenetic machineries influences the development of metabolic disorders such as diabetes and obesity; then, we focus on discussing the notion that manipulating metabolites, the fuel of cell metabolism, can function as a strategy for interfering epigenetic machinery and its related disease progression as well. PMID:27695375

  5. Architected Cellular Materials

    Science.gov (United States)

    Schaedler, Tobias A.; Carter, William B.

    2016-07-01

    Additive manufacturing enables fabrication of materials with intricate cellular architecture, whereby progress in 3D printing techniques is increasing the possible configurations of voids and solids ad infinitum. Examples are microlattices with graded porosity and truss structures optimized for specific loading conditions. The cellular architecture determines the mechanical properties and density of these materials and can influence a wide range of other properties, e.g., acoustic, thermal, and biological properties. By combining optimized cellular architectures with high-performance metals and ceramics, several lightweight materials that exhibit strength and stiffness previously unachievable at low densities were recently demonstrated. This review introduces the field of architected materials; summarizes the most common fabrication methods, with an emphasis on additive manufacturing; and discusses recent progress in the development of architected materials. The review also discusses important applications, including lightweight structures, energy absorption, metamaterials, thermal management, and bioscaffolds.

  6. Epigenetics and Cellular Metabolism

    Science.gov (United States)

    Xu, Wenyi; Wang, Fengzhong; Yu, Zhongsheng; Xin, Fengjiao

    2016-01-01

    Living eukaryotic systems evolve delicate cellular mechanisms for responding to various environmental signals. Among them, epigenetic machinery (DNA methylation, histone modifications, microRNAs, etc.) is the hub in transducing external stimuli into transcriptional response. Emerging evidence reveals the concept that epigenetic signatures are essential for the proper maintenance of cellular metabolism. On the other hand, the metabolite, a main environmental input, can also influence the processing of epigenetic memory. Here, we summarize the recent research progress in the epigenetic regulation of cellular metabolism and discuss how the dysfunction of epigenetic machineries influences the development of metabolic disorders such as diabetes and obesity; then, we focus on discussing the notion that manipulating metabolites, the fuel of cell metabolism, can function as a strategy for interfering epigenetic machinery and its related disease progression as well.

  7. Políticas de los cuerpos y emociones en los sujetos involucrados en acciones colectivas en la ciudad de Villa María

    Directory of Open Access Journals (Sweden)

    Adrián Scribano

    2012-03-01

    Full Text Available Este trabajo pretende dar cuenta, parcialmente, de la estructura de las sensibilidades sociales elaboradas a partir de la crisis del 2001 en la Argentina, a través de una indagación sistemática desde la voz de los sujetos que participaron en acciones colectivas durante la aludida crisis en la ciudad de Villa María, Córdoba. En el marco de dicho objetivo se identifican, describen y analizan las estructuras de sensibilidad que persisten a través del tiempo configuradas por el miedo, la resignación, la impotencia y la parálisis.

  8. Daniel Ricardo Peñaranda Supelano (comp.), Contra viento y marea. Acciones colectivas de alto riesgo en la zonas rurales colombianas 1985-2005

    OpenAIRE

    Cruz Rodríguez, Edwin

    2015-01-01

    Esta obra se ocupa de explorar la relación entre las acciones colectivas y el conflicto armado en Colombia, entre 1985 y 2005, conceptualizando y proponiendo herramientas para hacer mensurable el riesgo en el que ellas se desarrollan. El libro, compilado por el profesor del Instituto de Estudios Políticos de la Universidad Nacional de Colombia, Ricardo Peñaranda, se estructura en dos partes, más introducción y conclusiones generales, y presenta los resultados de una investigación desarrollada...

  9. Decolonizando acciones públicas contra el feminicidio con cuerpos disidentes: el performance y la plataforma arte acción en Chiapas México

    OpenAIRE

    Brittany Chávez; Doris Difarnecio

    2014-01-01

    Proponemos una forma alternativa de denunciar y visibilizar la violencia feminicida en la región de Chiapas por medio de la plataforma de performance y activismo Arte Acción. Tres partes constituyen este artículo: la situación del feminicidio en Chiapas y nuestra metodología desde lo erótico, lo decolonial y lo pedagógico; la creación de la memoria colectiva a través de acciones públicas; y nuestro trabajo continuado con la plataforma como un modo de activismo alternativo.

  10. Decolonizando acciones públicas contra el feminicidio con cuerpos disidentes: el performance y la plataforma arte acción en Chiapas México

    Directory of Open Access Journals (Sweden)

    Brittany Chávez

    2014-12-01

    Full Text Available Proponemos una forma alternativa de denunciar y visibilizar la violencia feminicida en la región de Chiapas por medio de la plataforma de performance y activismo Arte Acción. Tres partes constituyen este artículo: la situación del feminicidio en Chiapas y nuestra metodología desde lo erótico, lo decolonial y lo pedagógico; la creación de la memoria colectiva a través de acciones públicas; y nuestro trabajo continuado con la plataforma como un modo de activismo alternativo.

  11. ¿Políticas públicas o acciones de gobierno turísticas en Ixtapan de la Sal? El caso del Cocdetur

    OpenAIRE

    Graciela Cruz Jiménez

    2008-01-01

    Los cambios en la forma de gobierno en México han impulsado, al menos en teoría, a las políticas públicas como un mecanismo para propiciar la participación de los distintos sectores de la sociedad en su diseño, implementación y evaluación, contrario a las tradicionales acciones de gobierno, asumidas en solitario por la autoridad pública. El Consejo Consultivo de Ixtapan de la Sal (Cocdetur) se erigió como un órgano de consulta tendiente a incorporar los principios de las políticas públicas a...

  12. De la prevención a la promoción cultural: acciones pedagógicas en contextos de consumo problemático de drogas

    OpenAIRE

    Martin, Luján María Fabiana

    2011-01-01

    La mejor forma de prevenir el abuso de las drogas es no ocuparse de las drogas en sí, sino de todo lo demás: el hueco social en que la droga se instala señalando con su presencia otras carencias. Este corrimiento conceptual, desde la droga hacia lo que subyace al consumo en la persona y su grupo social, es el punto de partida de la prevención como promoción, como elemento constitutivo de la educación social. Así, como educadores, es posible tomar distancia de la prevención y proponer acciones...

  13. Acciones socioeducativas sobre sexualidad para la familia de infantes con parálisis cerebral en el Centro Internacional de Restauración Neurológica

    OpenAIRE

    Grethel Ortiz-Antón

    2011-01-01

    Esta investigación tiene como objetivo la propuesta de acciones socioeducativas dirigida a las madres y padres de infantes diagnosticados con parálisis cerebral, que asistieron a la Clínica de Atención a Pacientes Cubanos del CIREN en el periodo de mayo de 2009 a febrero de 2010 para recibir el Programa de Rehabilitación Multifactorial Intensiva. Se efectuó un estudio descriptivo de corte transversal, con una perspectiva cualitativa, que determinó la percepción de madres y padres acerca de la...

  14. Cellular Response to Irradiation

    Institute of Scientific and Technical Information of China (English)

    LIU Bo; YAN Shi-Wei

    2011-01-01

    To explore the nonlinear activities of the cellular signaling system composed of one transcriptional arm and one protein-interaction arm, we use an irradiation-response module to study the dynamics of stochastic interactions.It is shown that the oscillatory behavior could be described in a unified way when the radiation-derived signal and noise are incorporated.

  15. The New Cellular Immunology

    Science.gov (United States)

    Claman, Henry N.

    1973-01-01

    Discusses the nature of the immune response and traces many of the discoveries that have led to the present state of knowledge in immunology. The new cellular immunology is directing its efforts toward improving health by proper manipulation of the immune mechanisms of the body. (JR)

  16. Acciones para la sostenibilidad. El programa LIFE en el noroeste de la región de Murcia (España

    Directory of Open Access Journals (Sweden)

    Mercedes Millán Escriche

    2009-01-01

    Full Text Available El progreso de las sociedades desarrolladas ocasiona, con frecuencia, efectos indeseados en recursos cuya escasez y fragilidad los convierte en elementos de alto valor para nuestra generación y las futuras. Paliar y, mejor aún, evitar comportamientos nocivos para la sostenibilidad se ha convertido en un auténtico reto para esas mismas sociedades, dando origen a numerosas cumbres y encuentros de los que se derivan planes, programas y agendas, cuya misión es elaborar estrategias que contribuyan a mantener un mundo más habitable para todos. Este es también el principal objetivo del Programa de la Unión Europea LIFE, cuyas acciones se llevan a cabo en distintos territorios europeos y pretende que los resultados actúen como efecto demostrativo para otros tantos. En este trabajo se ponen de relieve las acciones del Programa LIFE en el Noroeste de la Región de Murcia (España, partiendo de la revisión de los documentos emitidos por las administraciones implicadas y observando su cumplimiento en el territorio afectado.

  17. Cellular host responses to gliomas.

    Directory of Open Access Journals (Sweden)

    Joseph Najbauer

    Full Text Available BACKGROUND: Glioblastoma multiforme (GBM is the most aggressive type of malignant primary brain tumors in adults. Molecular and genetic analysis has advanced our understanding of glioma biology, however mapping the cellular composition of the tumor microenvironment is crucial for understanding the pathology of this dreaded brain cancer. In this study we identified major cell populations attracted by glioma using orthotopic rodent models of human glioma xenografts. Marker-specific, anatomical and morphological analyses revealed a robust influx of host cells into the main tumor bed and tumor satellites. METHODOLOGY/PRINCIPAL FINDINGS: Human glioma cell lines and glioma spheroid orthotopic implants were used in rodents. In both models, the xenografts recruited large numbers of host nestin-expressing cells, which formed a 'network' with glioma. The host nestin-expressing cells appeared to originate in the subventricular zone ipsilateral to the tumor, and were clearly distinguishable from pericytes that expressed smooth muscle actin. These distinct cell populations established close physical contact in a 'pair-wise' manner and migrated together to the deeper layers of tumor satellites and gave rise to tumor vasculature. The GBM biopsy xenografts displayed two different phenotypes: (a low-generation tumors (first in vivo passage in rats were highly invasive and non-angiogenic, and host nestin-positive cells that infiltrated into these tumors displayed astrocytic or elongated bipolar morphology; (b high-generation xenografts (fifth passage had pronounced cellularity, were angiogenic with 'glomerulus-like' microvascular proliferations that contained host nestin-positive cells. Stromal cell-derived factor-1 and its receptor CXCR4 were highly expressed in and around glioma xenografts, suggesting their role in glioma progression and invasion. CONCLUSIONS/SIGNIFICANCE: Our data demonstrate a robust migration of nestin-expressing host cells to glioma, which

  18. Molecular and Cellular Signaling

    CERN Document Server

    Beckerman, Martin

    2005-01-01

    A small number of signaling pathways, no more than a dozen or so, form a control layer that is responsible for all signaling in and between cells of the human body. The signaling proteins belonging to the control layer determine what kinds of cells are made during development and how they function during adult life. Malfunctions in the proteins belonging to the control layer are responsible for a host of human diseases ranging from neurological disorders to cancers. Most drugs target components in the control layer, and difficulties in drug design are intimately related to the architecture of the control layer. Molecular and Cellular Signaling provides an introduction to molecular and cellular signaling in biological systems with an emphasis on the underlying physical principles. The text is aimed at upper-level undergraduates, graduate students and individuals in medicine and pharmacology interested in broadening their understanding of how cells regulate and coordinate their core activities and how diseases ...

  19. Magnetic Cellular Switches

    OpenAIRE

    Overby, Darryl R.; Alenghat, Francis J.; Montoya-Zavala, Martín; Bei, HuCheng; Oh, Philmo; Karavitis, John; Ingber, Donald E.

    2004-01-01

    This paper focuses on the development of magnetic cellular switches to enable magnetic control of intracellular functions in living mammalian cells, including receptor signal transduction and gene transcription. Our approach takes advantage of the mechanosensitivity of adenosine 3′,5′-monophosphate (cAMP) induction and downstream transcription controlled by the cAMP regulatory element (CRE) to engineer gene constructs that optically report gene expression in living cells. We activate transcri...

  20. Cellular therapy in Tuberculosis

    Directory of Open Access Journals (Sweden)

    Shreemanta K. Parida

    2015-03-01

    Full Text Available Cellular therapy now offer promise of potential adjunct therapeutic options for treatment of drug-resistant tuberculosis (TB. We review here the role of Mesenchymal stromal cells, (MSCs, as well as other immune effector cells in the therapy of infectious diseases with a focus on TB. MSCs represent a population of tissue-resident non-hematopoietic adult progenitor cells which home into injured tissues increase the proliferative potential of broncho-alveolar stem cells and restore lung epithelium. MSCs have been shown to be immune-modulatory and anti-inflammatory mediated via cell-cell contacts as well as soluble factors. We discuss the functional profile of MSCs and their potential use for adjunct cellular therapy of multi-drug resistant TB, with the aim of limiting tissue damage, and to convert unproductive inflammatory responses into effective anti-pathogen directed immune responses. Adjunct cellular therapy could potentially offer salvage therapy options for patients with drug-resistant TB, increase clinically relevant anti-M.tuberculosis directed immune responses and possibly shorten the duration of anti-TB therapy.

  1. Cellular Therapy for Heart Failure.

    Science.gov (United States)

    Psaltis, Peter J; Schwarz, Nisha; Toledo-Flores, Deborah; Nicholls, Stephen J

    2016-01-01

    The pathogenesis of cardiomyopathy and heart failure (HF) is underpinned by complex changes at subcellular, cellular and extracellular levels in the ventricular myocardium. For all of the gains that conventional treatments for HF have brought to mortality and morbidity, they do not adequately address the loss of cardiomyocyte numbers in the remodeling ventricle. Originally conceived to address this problem, cellular transplantation for HF has already gone through several stages of evolution over the past two decades. Various cell types and delivery routes have been implemented to positive effect in preclinical models of ischemic and nonischemic cardiomyopathy, with pleiotropic benefits observed in terms of myocardial remodeling, systolic and diastolic performance, perfusion, fibrosis, inflammation, metabolism and electrophysiology. To a large extent, these salubrious effects are now attributed to the indirect, paracrine capacity of transplanted stem cells to facilitate endogenous cardiac repair processes. Promising results have also followed in early phase human studies, although these have been relatively modest and somewhat inconsistent. This review details the preclinical and clinical evidence currently available regarding the use of pluripotent stem cells and adult-derived progenitor cells for cardiomyopathy and HF. It outlines the important lessons that have been learned to this point in time, and balances the promise of this exciting field against the key challenges and questions that still need to be addressed at all levels of research, to ensure that cell therapy realizes its full potential by adding to the armamentarium of HF management. PMID:27280304

  2. Environment Aware Cellular Networks

    KAUST Repository

    Ghazzai, Hakim

    2015-02-01

    The unprecedented rise of mobile user demand over the years have led to an enormous growth of the energy consumption of wireless networks as well as the greenhouse gas emissions which are estimated currently to be around 70 million tons per year. This significant growth of energy consumption impels network companies to pay huge bills which represent around half of their operating expenditures. Therefore, many service providers, including mobile operators, are looking for new and modern green solutions to help reduce their expenses as well as the level of their CO2 emissions. Base stations are the most power greedy element in cellular networks: they drain around 80% of the total network energy consumption even during low traffic periods. Thus, there is a growing need to develop more energy-efficient techniques to enhance the green performance of future 4G/5G cellular networks. Due to the problem of traffic load fluctuations in cellular networks during different periods of the day and between different areas (shopping or business districts and residential areas), the base station sleeping strategy has been one of the main popular research topics in green communications. In this presentation, we present several practical green techniques that provide significant gains for mobile operators. Indeed, combined with the base station sleeping strategy, these techniques achieve not only a minimization of the fossil fuel consumption but also an enhancement of mobile operator profits. We start with an optimized cell planning method that considers varying spatial and temporal user densities. We then use the optimal transport theory in order to define the cell boundaries such that the network total transmit power is reduced. Afterwards, we exploit the features of the modern electrical grid, the smart grid, as a new tool of power management for cellular networks and we optimize the energy procurement from multiple energy retailers characterized by different prices and pollutant

  3. Clinical applications of cellular therapy products

    OpenAIRE

    Serpil Yanbakan

    2015-01-01

    Adult stem cells have the potential to differentiate into multiple cell types and have usage about lots of regenerative medicine research fields. Especially bone marrow-derived mesenchymal stem cells have a wide range of case presentation. New discoveries about stem cell biology will progress new options about cellular therapy products and isolation of different stem cell types will increase hope for treatment of important illness such as Parkinson’s disease, diabetes, malign brain tumors. It...

  4. Acciones estratégicas de las empresas de familia comercializadoras de arroz de la ciudad de Villavicencio: Casos de estudio de las empresas Inproarroz Ltda., y Agrocom Ltda.

    OpenAIRE

    2010-01-01

    El presente documento ilustra, mediante un proceso de deducción, cuáles han sido las acciones estratégicas empleadas por las empresas de familia comercializadoras de arroz de la ciudad de Villavicencio, en el departamento del Meta, empresas cuya trayectoria y solidez demostradas a través del tiempo han dejado gran huella y un modelo para las empresas de familia. Es importante porque la mayoría de las empresas comercializadoras de arroz son familiares y el identificar las acciones estratégicas...

  5. Cellular Delivery of RNA Nanoparticles.

    Science.gov (United States)

    Parlea, Lorena; Puri, Anu; Kasprzak, Wojciech; Bindewald, Eckart; Zakrevsky, Paul; Satterwhite, Emily; Joseph, Kenya; Afonin, Kirill A; Shapiro, Bruce A

    2016-09-12

    RNA nanostructures can be programmed to exhibit defined sizes, shapes and stoichiometries from naturally occurring or de novo designed RNA motifs. These constructs can be used as scaffolds to attach functional moieties, such as ligand binding motifs or gene expression regulators, for nanobiology applications. This review is focused on four areas of importance to RNA nanotechnology: the types of RNAs of particular interest for nanobiology, the assembly of RNA nanoconstructs, the challenges of cellular delivery of RNAs in vivo, and the delivery carriers that aid in the matter. The available strategies for the design of nucleic acid nanostructures, as well as for formulation of their carriers, make RNA nanotechnology an important tool in both basic research and applied biomedical science. PMID:27509068

  6. Thermomechanical characterisation of cellular rubber

    Science.gov (United States)

    Seibert, H.; Scheffer, T.; Diebels, S.

    2016-09-01

    This contribution discusses an experimental possibility to characterise a cellular rubber in terms of the influence of multiaxiality, rate dependency under environmental temperature and its behaviour under hydrostatic pressure. In this context, a mixed open and closed cell rubber based on an ethylene propylene diene monomer is investigated exemplarily. The present article intends to give a general idea of the characterisation method and the considerable effects of this special type of material. The main focus lies on the experimental procedure and the used testing devices in combination with the analysis methods such as true three-dimensional digital image correlation. The structural compressibility is taken into account by an approach for a material model using the Theory of Porous Media with additional temperature dependence.

  7. Novel Materials for Cellular Nanosensors

    DEFF Research Database (Denmark)

    Sasso, Luigi

    modifications for electrochemical nanosensors for the detection of analytes released from cells. Two type of materials were investigated, each pertaining to the two different aspects of such devices: peptide nanostructures were studied for the creation of cellular sensing substrates that mimic in vivo surfaces......' functionalization with biomolecules, metal nanoparticles and chemical functional groups such as thiols, showing the versatility and flexibility of this material's applications. A technique for the patterning of these nanostructures using soft lithography was also developed and tested for suitable cell sensing....... An in vivo investigation also gave evidence of how the peptide nanowires can be used as surface modification in implantable electrodes for neurological measurements. Conducting polymers were utilized in electrode modifications for electrochemical sensor surfaces. Both chemical and electrochemical deposition...

  8. Cellular basis of memory for addiction.

    Science.gov (United States)

    Nestler, Eric J

    2013-12-01

    DESPITE THE IMPORTANCE OF NUMEROUS PSYCHOSOCIAL FACTORS, AT ITS CORE, DRUG ADDICTION INVOLVES A BIOLOGICAL PROCESS: the ability of repeated exposure to a drug of abuse to induce changes in a vulnerable brain that drive the compulsive seeking and taking of drugs, and loss of control over drug use, that define a state of addiction. Here, we review the types of molecular and cellular adaptations that occur in specific brain regions to mediate addiction-associated behavioral abnormalities. These include alterations in gene expression achieved in part via epigenetic mechanisms, plasticity in the neurophysiological functioning of neurons and synapses, and associated plasticity in neuronal and synaptic morphology mediated in part by altered neurotrophic factor signaling. Each of these types of drug-induced modifications can be viewed as a form of "cellular or molecular memory." Moreover, it is striking that most addiction-related forms of plasticity are very similar to the types of plasticity that have been associated with more classic forms of "behavioral memory," perhaps reflecting the finite repertoire of adaptive mechanisms available to neurons when faced with environmental challenges. Finally, addiction-related molecular and cellular adaptations involve most of the same brain regions that mediate more classic forms of memory, consistent with the view that abnormal memories are important drivers of addiction syndromes. The goal of these studies which aim to explicate the molecular and cellular basis of drug addiction is to eventually develop biologically based diagnostic tests, as well as more effective treatments for addiction disorders. PMID:24459410

  9. Engineering Cellular Metabolism

    DEFF Research Database (Denmark)

    Nielsen, Jens; Keasling, Jay

    2016-01-01

    of metabolic engineering and will discuss how new technologies can enable metabolic engineering to be scaled up to the industrial level, either by cutting off the lines of control for endogenous metabolism or by infiltrating the system with disruptive, heterologous pathways that overcome cellular regulation.......Metabolic engineering is the science of rewiring the metabolism of cells to enhance production of native metabolites or to endow cells with the ability to produce new products. The potential applications of such efforts are wide ranging, including the generation of fuels, chemicals, foods, feeds...

  10. Cellular Signaling in Health and Disease

    CERN Document Server

    Beckerman, Martin

    2009-01-01

    In today’s world, three great classes of non-infectious diseases – the metabolic syndromes (such as type 2 diabetes and atherosclerosis), the cancers, and the neurodegenerative disorders – have risen to the fore. These diseases, all associated with increasing age of an individual, have proven to be remarkably complex and difficult to treat. This is because, in large measure, when the cellular signaling pathways responsible for maintaining homeostasis and health of the body become dysregulated, they generate equally stable disease states. As a result the body may respond positively to a drug, but only for a while and then revert back to the disease state. Cellular Signaling in Health and Disease summarizes our current understanding of these regulatory networks in the healthy and diseased states, showing which molecular components might be prime targets for drug interventions. This is accomplished by presenting models that explain in mechanistic, molecular detail how a particular part of the cellular sign...

  11. Acciones colectivas como práctica de memoria, realizadas por una organización de víctimas en Medellín (Colombia

    Directory of Open Access Journals (Sweden)

    Jolyn Elena Castrillón

    2016-07-01

    Full Text Available La reconstrucción de los relatos de las víctimas del conflicto armado en Colombia es una herramienta para el fortalecimiento del tejido social; esta premisa orientó la investigación que tuvo como objetivo comprender los significados y sentidos de las acciones de memoria colectiva, realizadas por personas pertenecientes a una organización de víctimas de Medellín. El estudio se realizó desde un enfoque de investigación interpretativo con diseño cualitativo, fundamentado en el método fenomenológico-hermenéutico. Las técnicas de generación de información empleadas fueron la construcción de historias de vida, talleres participativos y entrevistas semi-estructuradas. Los hallazgos refieren que el apoyo del grupo familiar es fundamental, pues establece significantes biográficos que facilitan el proceso de construcción de memoria. Además, el apoyo grupal favorece el afrontamiento del dolor, la soledad, la tristeza y la desolación. El recuerdo es importante para la elaboración del duelo, más aún si se reconstruye junto con otros. El dolor se reconoce como parte de la experiencia vivida, pero no se acepta la inacción, puesto que la búsqueda de la verdad y la reparación son los horizontes de las acciones colectivas.

  12. LA PESQUISA Y DESARROLLO Y LOS PRECIOS DE LAS ACCIONES DE LAS EMPRESAS BRASILEÑAS: UN ESTUDIO EMPÍRICO EN LA BOVESPA

    Directory of Open Access Journals (Sweden)

    Arildo Hungarato

    2012-09-01

    Full Text Available El presente estudio tiene por objetivo identificar la relación entre los gastos en Pesquisa y Desarrollo (P&D com el precio de las acciones de las empresas brasileñas registradas en la Bovespa dando continuidad a los estudios de Lopes (2001;2002 y Rezende (2005 sobre value-relevance de la información contable en el Brasil. Esta pesquisa empírico-análitica fue basada en el modelo de Collins et al. (1997, que es una proxy del modelo Residual Income Valuation (RIV de Ohlson (1995 y en la clasificación de intensidad tecnológica de la pesquisa de Chan et al. (1990, realizada en los Estados Unidos de América. Se tomaron como muestra las empresas brasileñas registradas en la Bovespa, abarcando el período de 1996 a 2006. Por medio de regresiones múltiples, se identificó que los gastos en P&D no son estadisticamente significantes para el precio de las acciones de las empresas estudiadas. Tales conclusiones no ratifican la pesquisa de Chan et al. (1990, pero refuerzan los estudios de Ohlson (1995, Lopes (2001; 2002 y complementan el estudio de Rezende (2005, una vez que el estudio evidencia que el lucro continúa estadísticamente significante para el precio de la acción, presentando relación positiva incluso después de la deducción de los gastos en P&D contabilizados al gasto. Situación diferente ocurre para el PL (Patrimonio Líquido, que dejó de ser estadísticamente significante y de presentar relación con el precio de la acción, después de ser deducidos los gastos en P&D contabilizados como inversión.

  13. Componente genérico para la planificación y ejecución de acciones en aplicaciones de software

    Directory of Open Access Journals (Sweden)

    Guillermo Luzua Farias

    2013-06-01

    Full Text Available De todas las actividades y procesos que se implementan dentro de cualquier entidad, se puede afirmar que el proceso de planificación es un eslabón clave dentro de los resultados que se obtienen y el nivel de efectividad que se alcanza en los mismos. En este proceso es donde se acota y define la visión del objetivo que se quiere obtener. También es donde se hace un análisis del entorno donde se debe incidir y como proyectarse hacia el mismo. Además, se crean las metas u objetivos que se quieren alcanzar y se establecen cuáles son las acciones que se deben realizar para satisfacer los mismos. La planificación también incluye la definición del tiempo de inicio y fin de una determinada tarea. En el Centro de Desarrollo Geoinformática y Señales Digitales de la Universidad de las Ciencias Informáticas se creó un Componente de Gestión de Planificaciones Genéricas que gestiona todo el proceso de planificación de las tareas a ejecutar por una aplicación de software. Este componente es capaz de planificar tareas asociadas a los recursos y acciones que posea la aplicación de software. Al mismo tiempo, cuando una tarea debe de iniciarse, el componente se encarga de ordenar al software la ejecución de la misma. El componente automatiza la ejecución de los procesos planificados de un software, reduciendo el gasto de recursos humanos en la ejecución manual de estas tareas.

  14. Acciones socioeducativas sobre sexualidad para la familia de infantes con parálisis cerebral en el Centro Internacional de Restauración Neurológica

    Directory of Open Access Journals (Sweden)

    Grethel Ortiz-Antón

    2011-12-01

    Full Text Available Esta investigación tiene como objetivo la propuesta de acciones socioeducativas dirigida a las madres y padres de infantes diagnosticados con parálisis cerebral, que asistieron a la Clínica de Atención a Pacientes Cubanos del CIREN en el periodo de mayo de 2009 a febrero de 2010 para recibir el Programa de Rehabilitación Multifactorial Intensiva. Se efectuó un estudio descriptivo de corte transversal, con una perspectiva cualitativa, que determinó la percepción de madres y padres acerca de la sexualidad de sus hijas/os. Se aplicó una encuesta y se utilizó la técnica cualitativa de entrevista en profundidad para realizar el estudio. El diagnóstico demostró que existen vacíos en el conocimiento en la esfera de la sexualidad desde el rol de madres y padres, limitándose el conocimiento sobre esta temática permeado de mitos y prejuicios. No es suficiente la información recibida sobre los temas de la sexualidad infantil y consideran que la educación sexual de niñas/os que presentan alguna discapacidad puede ser necesaria si se condiciona a la recuperación física. En tanto, expresan que la sociedad no está preparada para convivir con la diferencia. La elaboración de las acciones socioeducativas se fundamentó a partir de la información obtenida en el proceso diagnóstico.

  15. Integrated cellular systems

    Science.gov (United States)

    Harper, Jason C.

    The generation of new three-dimensional (3D) matrices that enable integration of biomolecular components and whole cells into device architectures, without adversely altering their morphology or activity, continues to be an expanding and challenging field of research. This research is driven by the promise that encapsulated biomolecules and cells can significantly impact areas as diverse as biocatalysis, controlled delivery of therapeutics, environmental and industrial process monitoring, early warning of warfare agents, bioelectronics, photonics, smart prosthetics, advanced physiological sensors, portable medical diagnostic devices, and tissue/organ replacement. This work focuses on the development of a fundamental understanding of the biochemical and nanomaterial mechanisms that govern the cell directed assembly and integration process. It was shown that this integration process relies on the ability of cells to actively develop a pH gradient in response to evaporation induced osmotic stress, which catalyzes silica condensation within a thin 3D volume surrounding the cells, creating a functional bio/nano interface. The mechanism responsible for introducing functional foreign membrane-bound proteins via proteoliposome addition to the silica-lipid-cell matrix was also determined. Utilizing this new understanding, 3D cellular immobilization capabilities were extended using sol-gel matrices endowed with glycerol, trehalose, and media components. The effects of these additives, and the metabolic phase of encapsulated S. cerivisiase cells, on long-term viability and the rate of inducible gene expression was studied. This enabled the entrapment of cells within a novel microfluidic platform capable of simultaneous colorimetric, fluorescent, and electrochemical detection of a single analyte, significantly improving confidence in the biosensor output. As a complementary approach, multiphoton protein lithography was utilized to engineer 3D protein matrices in which to

  16. A structural and functional homolog supports a general role for frataxin in cellular iron chemistry.

    Science.gov (United States)

    Qi, Wenbin; Cowan, J A

    2010-02-01

    Bacillus subtilis YdhG lacks sequence homology, but demonstrates structural and functional similarity to the frataxin family, supporting a general cellular role for frataxin-type proteins in cellular iron homeostasis.

  17. Multiuser Cellular Network

    CERN Document Server

    Bao, Yi; Chen, Ming

    2011-01-01

    Modern radio communication is faced with a problem about how to distribute restricted frequency to users in a certain space. Since our task is to minimize the number of repeaters, a natural idea is enlarging coverage area. However, coverage has restrictions. First, service area has to be divided economically as repeater's coverage is limited. In this paper, our fundamental method is to adopt seamless cellular network division. Second, underlying physics content in frequency distribution problem is interference between two close frequencies. Consequently, we choose a proper frequency width of 0.1MHz and a relevantly reliable setting to apply one frequency several times. We make a few general assumptions to simplify real situation. For instance, immobile users yield to homogenous distribution; repeaters can receive and transmit information in any given frequency in duplex operation; coverage is mainly decided by antenna height. Two models are built up to solve 1000 users and 10000 users situations respectively....

  18. Cellular Phone Towers

    Science.gov (United States)

    ... Cancer? Breast Cancer Colon/Rectum Cancer Lung Cancer Prostate Cancer Skin Cancer Show All Cancer Types News and Features Cancer Glossary ACS Bookstore Cancer Information Cancer Basics Cancer Prevention & Detection Signs & Symptoms of Cancer Treatments & Side Effects ...

  19. Never-ageing cellular senescence

    OpenAIRE

    Ogrunc, Müge; d’Adda di Fagagna, Fabrizio

    2011-01-01

    Cellular senescence was historically discovered as a form of cellular ageing of in vitro cultured cells. It has been under the spotlight following the evidence of oncogene-induced senescence in vivo and its role as a potent tumour suppressor mechanism. Presently, a PubMed search using keywords ‘cellular senescence and cancer’ reveals 8398 number of references (by April 2011) showing that while our knowledge of senescence keeps expanding, the complexity of the phenomenon keeps us – researchers...

  20. Cellular bioluminescence imaging.

    Science.gov (United States)

    Welsh, David K; Noguchi, Takako

    2012-08-01

    Bioluminescence imaging of live cells has recently been recognized as an important alternative to fluorescence imaging. Fluorescent probes are much brighter than bioluminescent probes (luciferase enzymes) and, therefore, provide much better spatial and temporal resolution and much better contrast for delineating cell structure. However, with bioluminescence imaging there is virtually no background or toxicity. As a result, bioluminescence can be superior to fluorescence for detecting and quantifying molecules and their interactions in living cells, particularly in long-term studies. Structurally diverse luciferases from beetle and marine species have been used for a wide variety of applications, including tracking cells in vivo, detecting protein-protein interactions, measuring levels of calcium and other signaling molecules, detecting protease activity, and reporting circadian clock gene expression. Such applications can be optimized by the use of brighter and variously colored luciferases, brighter microscope optics, and ultrasensitive, low-noise cameras. This article presents a review of how bioluminescence differs from fluorescence, its applications to cellular imaging, and available probes, optics, and detectors. It also gives practical suggestions for optimal bioluminescence imaging of single cells.

  1. Evaluación de acciones explosivas sobre estructuras de hormigón armado mediante elementos finitos

    OpenAIRE

    Bermejo, M.; Santos, A. P.; Goicolea, J. M.; Pérez, A.

    2015-01-01

    Explosive phenomena on concrete structures have catastrophic effects in many instances despite its reduced occurrence. The civil buildings are usually not designed to withstand this type of dynamic load, so a methodology to analyze the structural response on blast loads is recommended. This paper studies the behavior of reinforced concrete frame structures against these actions by lagrangian finite elements method with explicit time integration. Finite element models of segregated concrete an...

  2. CONAE's actions for the promotion of photovoltaic systems; Acciones de la CONAE para la promocion de sistemas fotovoltaicos

    Energy Technology Data Exchange (ETDEWEB)

    Hungler Salceda, Federico [Comision Nacional para el Ahorro de Energia (CONAE), Mexico, (Mexico)

    2007-06-15

    In this presentation, there are found all the processes -concerning the renewable energy- carried out by the National Commission for Energy Savings (CONAE). Firstly, it is described the role playing by the CONAE on different premises and departments either federal or private. It is found a list of those current actions carried out, among there are going to be found: to be part of the consulting council that fosters the renewable energy, to organize varied activities not only at national level but also at international level - diplomat courses, workshops, etc. - Then, it is briefly described the agreement between the Conae and the Shared Risk Trust Fund (FIRCO). In addition, it is explained the program, whose major purposes are: on the one hand, to decrease the energy expenditure, and on the other, to increase the efficient use of the energy. Generally speaking, it is shown the economic expenditure of different materials used in rural communities either in order to cook the food or to produce artificial light. Besides, there are described the current technological alternatives. There are also shown some comparison tables with outstanding information about either the materials producing light or the use cost of a photovoltaic panel. Finally, there are explained the actions that the Conae can carry out in order to promote the photovoltaic panels interconnected to the electric network. [Spanish] En esta presentacion se describen los procesos que realiza la Comision Nacional para el Ahorro de Energia (CONAE) en relacion con la energia renovable. En primer plano, se describe el papel que desempena la CONAE en diversas dependencias y departamentos no solo federales sino tambien particulares. Asimismo, se enumeran las acciones que realiza actualmente, entre las que se pueden encontrar: formar parte del consejo de consulta para el fomento de energias renovables, organizar diversas actividades a nivel nacional e internacional. Mas adelante, se describe brevemente el convenio

  3. About Strongly Universal Cellular Automata

    Directory of Open Access Journals (Sweden)

    Maurice Margenstern

    2013-09-01

    Full Text Available In this paper, we construct a strongly universal cellular automaton on the line with 11 states and the standard neighbourhood. We embed this construction into several tilings of the hyperbolic plane and of the hyperbolic 3D space giving rise to strongly universal cellular automata with 10 states.

  4. Percepciones de los efectos de la implementación del Sistema General de Seguridad Social en Salud sobre las acciones de control de tuberculosis en el Valle del Cauca, Colombia.

    Directory of Open Access Journals (Sweden)

    Rocío Carvajal

    2009-11-01

    Full Text Available ANTECEDENTES: La reforma en salud en Colombia tuvo un profundo efecto en los programas de salud pública. Los cambios en la estructura organizativa y en las actividades de intervención en las acciones de control de la tuberculosis (TBC fue provocado por el surgimiento de nuevos actores en la prestación de servicios, el diseño de nuevos planes de salud, la diversificación y ambigüedad de responsabilidades y la descentralización del sistema. OBJETIVO: Conocer las percepciones que tienen actores clave del control de la tuberculosis acerca de los efectos generados por la implementación del Sistema General de Seguridad Social en Salud (SGSSS de Colombia. MATERIALES Y MÉTODOS: Se presentan los hallazgos cualitativos de un estudio de caso. La técnica de recolección de información fue la entrevista semi-estructurada. En total se entrevistaron 15 informantes clave, todos personal de salud responsables de las acciones de control de la tuberculosis en el ámbito nacional, departamental y local. RESULTADOS: Los efectos negativos más relevantes de la implementación del SGSSS en las acciones de control de la TBC fueron: falta de voluntad política, corrupción en el manejo de los recursos, pobre destreza en el desarrollo técnico-administrativo, deficiencia del Estado en las actividades de supervisión a nivel local, inadecuado cumplimiento del Estado en sus obligaciones administrativas y financieras. La diversificación de las fuentes de financiación para salud fue descrita como factor facilitante porque incrementa la distribución de los recursos en las acciones de control. DISCUSIÓN: La reducción del papel del Estado en las actividades de regulación y control fue percibido como el principal efecto. La introducción de nuevos actores en la entrega de servicios de salud y el aumento de la distribución de los recursos en las acciones de la TBC no dieron los efectos positivos esperados y la cantidad y calidad de las acciones se redujeron.

  5. MIMO Communication for Cellular Networks

    CERN Document Server

    Huang, Howard; Venkatesan, Sivarama

    2012-01-01

    As the theoretical foundations of multiple-antenna techniques evolve and as these multiple-input multiple-output (MIMO) techniques become essential for providing high data rates in wireless systems, there is a growing need to understand the performance limits of MIMO in practical networks. To address this need, MIMO Communication for Cellular Networks presents a systematic description of MIMO technology classes and a framework for MIMO system design that takes into account the essential physical-layer features of practical cellular networks. In contrast to works that focus on the theoretical performance of abstract MIMO channels, MIMO Communication for Cellular Networks emphasizes the practical performance of realistic MIMO systems. A unified set of system simulation results highlights relative performance gains of different MIMO techniques and provides insights into how best to use multiple antennas in cellular networks under various conditions. MIMO Communication for Cellular Networks describes single-user,...

  6. Two HAP2-GCS1 homologs responsible for gamete interactions in the cellular slime mold with multiple mating types: Implication for common mechanisms of sexual reproduction shared by plants and protozoa and for male-female differentiation.

    Science.gov (United States)

    Okamoto, Marina; Yamada, Lixy; Fujisaki, Yukie; Bloomfield, Gareth; Yoshida, Kentaro; Kuwayama, Hidekazu; Sawada, Hitoshi; Mori, Toshiyuki; Urushihara, Hideko

    2016-07-01

    Fertilization is a central event in sexual reproduction, and understanding its molecular mechanisms has both basic and applicative biological importance. Recent studies have uncovered the molecules that mediate this process in a variety of organisms, making it intriguing to consider conservation and evolution of the mechanisms of sexual reproduction across phyla. The social amoeba Dictyostelium discoideum undergoes sexual maturation and forms gametes under dark and humid conditions. It exhibits three mating types, type-I, -II, and -III, for the heterothallic mating system. Based on proteome analyses of the gamete membranes, we detected expression of two homologs of the plant fertilization protein HAP2-GCS1. When their coding genes were disrupted in type-I and type-II strains, sexual potency was completely lost, whereas disruption in the type-III strain did not affect mating behavior, suggesting that the latter acts as female in complex organisms. Our results demonstrate the highly conserved function of HAP2-GCS1 in gamete interactions and suggest the presence of additional allo-recognition mechanisms in D. discoideum gametes.

  7. Two HAP2-GCS1 homologs responsible for gamete interactions in the cellular slime mold with multiple mating types: Implication for common mechanisms of sexual reproduction shared by plants and protozoa and for male-female differentiation.

    Science.gov (United States)

    Okamoto, Marina; Yamada, Lixy; Fujisaki, Yukie; Bloomfield, Gareth; Yoshida, Kentaro; Kuwayama, Hidekazu; Sawada, Hitoshi; Mori, Toshiyuki; Urushihara, Hideko

    2016-07-01

    Fertilization is a central event in sexual reproduction, and understanding its molecular mechanisms has both basic and applicative biological importance. Recent studies have uncovered the molecules that mediate this process in a variety of organisms, making it intriguing to consider conservation and evolution of the mechanisms of sexual reproduction across phyla. The social amoeba Dictyostelium discoideum undergoes sexual maturation and forms gametes under dark and humid conditions. It exhibits three mating types, type-I, -II, and -III, for the heterothallic mating system. Based on proteome analyses of the gamete membranes, we detected expression of two homologs of the plant fertilization protein HAP2-GCS1. When their coding genes were disrupted in type-I and type-II strains, sexual potency was completely lost, whereas disruption in the type-III strain did not affect mating behavior, suggesting that the latter acts as female in complex organisms. Our results demonstrate the highly conserved function of HAP2-GCS1 in gamete interactions and suggest the presence of additional allo-recognition mechanisms in D. discoideum gametes. PMID:27189178

  8. Funciones y estrategias socioeducativas de los técnicos comunitarios en procesos de evaluación participativa de acciones comunitarias (EPAC (resumen de tesis

    Directory of Open Access Journals (Sweden)

    Héctor Núñez

    2014-12-01

    Full Text Available Contar con la implicación de diversos agentes en los procesos de evaluación en el ámbito comunitario es una realidad altamente aceptada en el contexto anglosajón; en donde fundaciones y organismos públicos que financian proyectos consideran que las evaluaciones basadas en la participación de todos los implicados dentro de un programa, sean éstos (a políticos, (b gestores, (c implementadores y (d usuarios pueden mejorar la calidad de los resultados de evaluación. Por otra parte, parece necesario que las organizaciones del ámbito comunitario y sus profesionales tengan competencias evaluadoras que permitan llevar a cabo acciones de evaluación de los programas que desarrollan, con el doble objetivo de: 1 gestionar mejor los recursos y 2 mejorar las intervenciones sociales y educativas planteadas.

  9. ¿Políticas públicas o acciones de gobierno turísticas en Ixtapan de la Sal? El caso del Cocdetur

    Directory of Open Access Journals (Sweden)

    Graciela Cruz Jiménez

    2008-01-01

    Full Text Available Los cambios en la forma de gobierno en México han impulsado, al menos en teoría, a las políticas públicas como un mecanismo para propiciar la participación de los distintos sectores de la sociedad en su diseño, implementación y evaluación, contrario a las tradicionales acciones de gobierno, asumidas en solitario por la autoridad pública. El Consejo Consultivo de Ixtapan de la Sal (Cocdetur se erigió como un órgano de consulta tendiente a incorporar los principios de las políticas públicas a la conducción de la actividad turística. Este artículo pretende determinar si se cumplieron estos propósitos o se mantuvo el esquema tradicional. Con base en ello, las condiciones sustentables o no que propicia una u otra en la comunidad receptora.

  10. Modelo DIL-D ©: diagnosticando las acciones de responsabilidad social corporativa centradas en la integración laboral de personas con discapacidad

    Directory of Open Access Journals (Sweden)

    Marina Romeo Delgado

    2016-06-01

    Full Text Available El principal objetivo de la presente investigación es analizar y diagnosticar el grado de implantación y desarrollo de las acciones de responsabilidad social corporativa que facilitan la integración laboral de personas con discapacidad (RSC-D. Para ello hemos desarrollado un modelo clasificatorio (Modelo DIL-D © y un instrumento diagnóstico que dota de herramientas objetivas a los profesionales, en aras de fomentar la integración laboral de personas con discapacidad. El instrumento desarrollado se ha administrado a responsables RSC y/o de RRHH de 42 empresas. Los resultados nos muestran la confiabilidad del instrumento, así como el ajuste del Modelo. Dos tercios de las empresas obtienen niveles altos de desarrollo en todas las dimensiones, si bien un 34,5 % bajos. El modelo establece las pautas de intervención que la empresa ordinaria debe seguir en aras de promover la integración laboral, facilitando las herramientas de diagnóstico que promueven la creación de entornos integradores.

  11. Alteraciones en el reconocimiento de gestos por daño a nivel del praxicon de entrada de acciones. (Agnosia de las pantomimas

    Directory of Open Access Journals (Sweden)

    Daniel G. Politis

    2002-01-01

    Full Text Available Clásicamente el estudio de la apraxia estuvo limitado a los problemas en la ejecución gestual (Geschwind, 1965; Liepmann, 1908 [c.f. De Renzi, 1990]; Luria, 1977. Si bien se presentaron varias hipótesis sobre la capacidad de reconocer gestos y su alteración por lesiones cerebrales, González Rothi, Ochipa y Heilman (1991,1997 en su modelo cognitivo de las apraxias de los miembros incluyen la capacidad de reconocer gestos y proponen que la misma es mediada por un sub-componente específico, el praxicón de entrada de acciones. La alteración de este produce un cuadro caracterizado por un déficit en el reconocimiento de gestos que denominaron Agnosia de las Pantomimas. En este trabajo se presenta el estudio de un paciente con dificultades en el reconocimiento de gestos y sin dificultades en la producción gesrual lo que constituye una disociación, que reafirma la hipótesis planteada por González Rothi y colaboradores (1991, 1997 y se discute el valor de estos hallazgos en relación con el modelo antes mencionado.

  12. Diagnóstico y acciones para la acreditación del laboratorio de ensayo de la Refinería Sergio Soto.

    Directory of Open Access Journals (Sweden)

    Senia Figueroa García

    2016-04-01

    Full Text Available La necesidad de resultados confiables en el laboratorio de ensayos de la empresa Refinería de Petróleo de Cabaiguán y la importancia de sus producciones como portadores energéticos utilizados fundamentalmente en el transporte automotor y la generación de energía eléctrica, constituyen las premisas de este trabajo, que propone un diagnóstico de la situación actual del laboratorio para enfrentar la acreditación. Se identifican las principales causas que influyen en este proceso, se utilizan herramientas básicas de la calidad; se destaca la tormenta de ideas, el Diagrama Causa-Efecto y de Pareto. El resultado muestra la necesidad de enfocar los esfuerzos en resolver los problemas relacionados con las causas de la categoría Equipos: falta de equipos y piezas de repuesto, equipos obsoletos y mantenimiento preventivo; también se destaca lo relativo a las causas de la categoría Métodos: falta de un Programa de Auditorías Internas, y de procedimientos documentados que aseguran la calidad de sus resultados. Se tomaron un grupo de acciones que contribuyen a resolver las dificultades detectadas, con vistas a solicitar la acreditación del laboratorio de ensayos a las autoridades competentes.

  13. Actions and results of the management of energy saving of the CONDUMEX group; Acciones y resultados de la gerencia de ahorro de energia de grupo CONDUMEX

    Energy Technology Data Exchange (ETDEWEB)

    Sashida Key, Carlos; Jimenez Huerta, Arturo; Alavez Alcazar, Moises [Grupo Condumex, Industrias IEM, S.A. de C.V., Mexico, D.F. (Mexico)

    1999-07-01

    The purpose of this paper is to expose some practical cases that have been developed through the Energy Saving Management of GRUPO CONDUMEX, in different areas such as systems related to water, steam, electricity and compressed air. This experience has been compiled of diagnoses and actions that have been carried out in the companies inside and outside GRUPO CONDUMEX, and of systems with the purpose of making efficient the use of fuels and getting close to the energy indexes that are handled in other International companies of the same manufacture branch. [Spanish] Este trabajo tiene por objeto exponer algunos casos practicos que se han desarrollado a traves de la Gerencia de Ahorro de Energia de GRUPO CONDUMEX, en distintas areas tales como sistemas relacionados con agua, vapor, electricidad y aire comprimido. Esta experiencia ha sido recopilada de diagnosticos y acciones que se han llevado a cabo en las empresas dentro y fuera de GRUPO CONDUMEX, y de sistemas con la finalidad de eficientar el uso de los energeticos y acercarnos a los indices energeticos que se manejan en otras empresas internacionales del mismo ramo de fabricacion.

  14. Acciones colectivas frente a la violencia: disquisiciones a partir de un estudio de casos: Comuna 13 de Medellín (Colombia

    Directory of Open Access Journals (Sweden)

    Mary Luz Alzate Zuluaga

    2012-11-01

    Full Text Available En este artículo se analizan las modalidades y estrategias de acción que los sujetos colectivos producen para enfrentar la violencia, a partir de un estudio de casos de acciones colectivas producidas en el contexto del conflicto interno armado colombiano, caracterizándolas como modalidades de acción política intentando revertir, transformar o alterar las relaciones de poder que a través de tal violencia se pretende implantar. Concluyendo en la emergencia de un nuevo escenario de lo público, que plantea la existencia de orientaciones y formas de acción opositoras a la dominación armada, e ilustradoras de una pluralidad de alternativas culturales, políticas y sociales civilistas y democráticas.This article discusses the modalities and strategies that produce collective subjects to deal with violence, from a case study of collective action produced in the context of the Colombian internal armed conflict, characterizing them as forms of political action trying to reverse, transform or alter the power relations through such violence is intended to implement. Ending in the emergence of a new stage of the public, positing the existence of guidelines and forms of action armed opposition to domination and illustrations of a plurality of alternative cultural, political and social civility and democratic.

  15. Microfluidic Devices for the Measurement of Cellular Secretion

    Science.gov (United States)

    Schrell, Adrian M.; Mukhitov, Nikita; Yi, Lian; Wang, Xue; Roper, Michael G.

    2016-06-01

    The release of chemical information from cells and tissues holds the key to understanding cellular behavior and dysfunction. The development of methodologies that can measure cellular secretion in a time-dependent fashion is therefore essential. Often these measurements are made difficult by the high-salt conditions of the cellular environment, the presence of numerous other secreted factors, and the small mass samples that are produced when frequent sampling is used to resolve secretory dynamics. In this review, the methods that we have developed for measuring hormone release from islets of Langerhans are dissected to illustrate the practical difficulties of studying cellular secretions. Other methods from the literature are presented that provide alternative approaches to particularly challenging areas of monitoring cellular secretion. The examples presented in this review serve as case studies and should be adaptable to other cell types and systems for unique applications.

  16. Cellular Dynamic Simulator: An Event Driven Molecular Simulation Environment for Cellular Physiology

    Science.gov (United States)

    Byrne, Michael J.; Waxham, M. Neal; Kubota, Yoshihisa

    2010-01-01

    In this paper, we present the Cellular Dynamic Simulator (CDS) for simulating diffusion and chemical reactions within crowded molecular environments. CDS is based on a novel event driven algorithm specifically designed for precise calculation of the timing of collisions, reactions and other events for each individual molecule in the environment. Generic mesh based compartments allow the creation / importation of very simple or detailed cellular structures that exist in a 3D environment. Multiple levels of compartments and static obstacles can be used to create a dense environment to mimic cellular boundaries and the intracellular space. The CDS algorithm takes into account volume exclusion and molecular crowding that may impact signaling cascades in small sub-cellular compartments such as dendritic spines. With the CDS, we can simulate simple enzyme reactions; aggregation, channel transport, as well as highly complicated chemical reaction networks of both freely diffusing and membrane bound multi-protein complexes. Components of the CDS are generally defined such that the simulator can be applied to a wide range of environments in terms of scale and level of detail. Through an initialization GUI, a simple simulation environment can be created and populated within minutes yet is powerful enough to design complex 3D cellular architecture. The initialization tool allows visual confirmation of the environment construction prior to execution by the simulator. This paper describes the CDS algorithm, design implementation, and provides an overview of the types of features available and the utility of those features are highlighted in demonstrations. PMID:20361275

  17. Acciones colectivas en el conflicto político colombiano: ¿De guerrilla a grupos terroristas? El caso del ELN Acciones colectivas en el conflicto político colombiano: ¿De guerrilla a grupos terroristas? El caso del ELN

    Directory of Open Access Journals (Sweden)

    Alba Nubia Rodríguez Pizarro

    2006-01-01

    Full Text Available El propósito del siguiente artículo es argumentar las limitaciones analíticas y estratégicas de las denominaciones: grupos guerrilleros y grupos terroristas, para algunas de las acciones colectivas que hacen parte del conflicto político colombiano, y señalar la importancia de conocer los procesos a través de los cuales se construye la acción colectivaThe purpose of the following article is to argument the analytic and strategic limitations of the denominations: guerrillas groups and terrorist groups, for some of the collective actions that make part of the Colombian political conflict and to point out the importance of knowing the processes throughout which collective action is built.

  18. Cellular systems biology profiling applied to cellular models of disease.

    Science.gov (United States)

    Giuliano, Kenneth A; Premkumar, Daniel R; Strock, Christopher J; Johnston, Patricia; Taylor, Lansing

    2009-11-01

    Building cellular models of disease based on the approach of Cellular Systems Biology (CSB) has the potential to improve the process of creating drugs as part of the continuum from early drug discovery through drug development and clinical trials and diagnostics. This paper focuses on the application of CSB to early drug discovery. We discuss the integration of protein-protein interaction biosensors with other multiplexed, functional biomarkers as an example in using CSB to optimize the identification of quality lead series compounds.

  19. La financiación por medio de acciones privilegiadas : una reflexión sobre su aplicación en algunas empresas ferroviarias españolas durante el siglo XX

    Directory of Open Access Journals (Sweden)

    Miguel Ángel Villacorta Hernández

    2009-12-01

    Full Text Available Históricamente, las empresas ferroviarias españolas se financiaron por medio de empréstitos, en menor medida mediante acciones ordinarias y, de forma casi testimonial, con híbridos financieros. Esta investigación pretende detallar las posibles ventajas que hubiera supuesto, para estas compañías, la financiación por medio de clases de acciones y otros híbridos financieros.Historically, the Spanish railway companies were financed through borrowing, to a lesser degree by stock and, almost testimonial with hybrid financing. This research aims to describe the potential benefits that would have led, to these companies, financing through class actions and other financial hybrids.

  20. Investigaccion-accion en la sala de clases sobre las creencias de la cultura de la ciencia de un grupo de estudiantes universitarios y su relacion reciproca con el aprendizaje de las ciencias biologicas

    Science.gov (United States)

    Cordova-Santiago, Lizzette Astrid

    La investigacion---accion que se llevo a cabo en la sala de clases tenia como punto de partida las creencias de la cultura de la ciencia de un grupo de estudiantes universitarios para luego examinar sus implicaciones en el proceso de aprendizaje de las Ciencias Biologicas. ¿Que se supone que hagan las creencias en relacion con el aprendizaje? ¿En que consiste incorporar este aspecto a la practica educativa universitaria? Utilizando el modelo de Kemmis y McTaggart (1987) la investigacion-accion se planteo como un proceso dinamico en cuatro momentos en espiral constituidos por la planificacion, la accion, la observacion y la reflexion. Cada una de las fases tuvo una intencion retrospectiva y prospectiva formando una espiral de autorreflexion del conocimiento y la accion. Se llevaron a cabo audio grabaciones en clases y analisis de documentos. Ademas, la profesora-investigadora hizo un portafolio para reflexionar sobre las creencias de la cultura de la ciencia que tienen los estudiantes y las creencias del aprendizaje que tiene la profesora y sobre como la comprension de estos elementos ayudo a mejorar su practica educativa a traves del tiempo. Los resultados obtenidos apuntan a que las creencias de la cultura de la ciencia que tiene el grupo de estudiantes son diversas. Ellos si creen que la ciencia tiene una cultura la cual describieron como: complicada y desconocida que evoluciona constantemente, que es un conjunto de metodos, que es altamente tecnologica, que resuelve problemas de salud, ayuda a interpretar la realidad del mundo que los rodea y su origen y que existen unas intersecciones entre la ciencia y el poder. Sobre las creencias del proceso de aprendizaje de la profesora-investigadora, estas senalan que el modelaje de actores, la vision de la academia que tiene ella asi como la participacion y negociacion entre todos los involucrados en el proceso educativo, son factores que inciden en el proceso de aprendizaje.

  1. Acciones metodológicas para la rotación: hematología en la asignatura práctica pre - profesional de Bioanálisis Clínico.

    Directory of Open Access Journals (Sweden)

    Oriol Meneses Echemendia

    2014-09-01

    Full Text Available LSe presentan acciones metodológicas para contribuir a la preparación didáctica de los docentes en la asignatura Hematología, en el plan de estudio de la carrera Licenciatura en Tecnología de la Salud, en la especialidad Laboratorio clínico, la cual aborda el tratamiento metodológico de cada uno de los componentes didácticos de las unidades del programa. En ella se establecen y precisan conceptos que enriquecen la metodología de la Enseñanza de la Hematología, lo que garantizan la aplicación de las acciones metodológicas diseñadas. En la investigación se emplearon diferentes métodos y técnicas de la investigación pedagógica en el nivel teórico, empírico y estadísticos matemáticos. La aplicación práctica de las acciones metodológicas, se direccionan a elevar la calidad del desempeño profesional del docente.

  2. Cellular mechanisms during vascular development

    OpenAIRE

    Blum, Yannick

    2012-01-01

    The vascular system is an essential organ in vertebrate animals and provides the organism with enough oxygen and nutrients. It is composed of an interconnected network of blood vessels, which form using a number of different morphogenetic mechanisms. Angiogenesis describes the formation of new blood vessels from preexisting vessels. A number of molecular pathways have been shown to be essential during angiogenesis. However, cellular architecture of blood vessels as well as cellular mechanisms...

  3. Cellular automaton for chimera states

    OpenAIRE

    García-Morales, Vladimir

    2016-01-01

    A minimalistic model for chimera states is presented. The model is a cellular automaton (CA) which depends on only one adjustable parameter, the range of the nonlocal coupling, and is built from elementary cellular automata and the majority (voting) rule. This suggests the universality of chimera-like behavior from a new point of view: Already simple CA rules based on the majority rule exhibit this behavior. After a short transient, we find chimera states for arbitrary initial conditions, the...

  4. Mathematical Modeling of Cellular Metabolism.

    Science.gov (United States)

    Berndt, Nikolaus; Holzhütter, Hermann-Georg

    2016-01-01

    Cellular metabolism basically consists of the conversion of chemical compounds taken up from the extracellular environment into energy (conserved in energy-rich bonds of organic phosphates) and a wide array of organic molecules serving as catalysts (enzymes), information carriers (nucleic acids), and building blocks for cellular structures such as membranes or ribosomes. Metabolic modeling aims at the construction of mathematical representations of the cellular metabolism that can be used to calculate the concentration of cellular molecules and the rates of their mutual chemical interconversion in response to varying external conditions as, for example, hormonal stimuli or supply of essential nutrients. Based on such calculations, it is possible to quantify complex cellular functions as cellular growth, detoxification of drugs and xenobiotic compounds or synthesis of exported molecules. Depending on the specific questions to metabolism addressed, the methodological expertise of the researcher, and available experimental information, different conceptual frameworks have been established, allowing the usage of computational methods to condense experimental information from various layers of organization into (self-) consistent models. Here, we briefly outline the main conceptual frameworks that are currently exploited in metabolism research.

  5. Isolation and characterization of TgVP1, a type I vacuolar H+-translocating pyrophosphatase from Toxoplasma gondii. The dynamics of its subcellular localization and the cellular effects of a diphosphonate inhibitor.

    Science.gov (United States)

    Drozdowicz, Yolanda M; Shaw, Michael; Nishi, Manami; Striepen, Boris; Liwinski, Helene A; Roos, David S; Rea, Philip A

    2003-01-10

    Here we report the isolation and characterization of a type I vacuolar-type H(+)-pyrophosphatase (V-PPase), TgVP1, from an apicomplexan, Toxoplasma gondii, a parasitic protist that is particularly amenable to molecular and genetic manipulation. The 816-amino acid TgVP1 polypeptide is 50% sequence-identical (65% similar) to the prototypical type I V-PPase from Arabidopsis thaliana, AVP1, and contains all the sequence motifs characteristic of this pump category. Unlike AVP1 and other known type I enzymes, however, TgVP1 contains a 74-residue N-terminal extension encompassing a 42-residue N-terminal signal peptide sequence, sufficient for targeting proteins to the secretory pathway of T. gondii. Providing that the coding sequence for the entire N-terminal extension is omitted from the plasmid, transformation of Saccharomyces cerevisiae with plasmid-borne TgVP1 yields a stable and functional translation product that is competent in aminomethylenediphosphonate (AMDP)-inhibitable K(+)-activated pyrophosphate (PP(i)) hydrolysis and PP(i)-energized H(+) translocation. Immunofluorescence microscopy of both free and intracellular T. gondii tachyzoites using purified universal V-PPase polyclonal antibodies reveals a punctate apical distribution for the enzyme. Equivalent studies of the tachyzoites during host cell invasion, by contrast, disclose a transverse radial distribution in which the V-PPase is associated with a collar-like structure that migrates along the length of the parasite in synchrony with and in close apposition to the penetration furrow. Although treatment of T. gondii with AMDP concentrations as high as 100 microm had no discernible effect on the efficiency of host cell invasion and integration, concentrations commensurate with the I(50) for the inhibition of TgVP1 activity in vitro (0.9 microm) do inhibit cell division and elicit nuclear enlargement concomitant with the inflation and eventual disintegration of acidocalcisome-like vesicular structures. A

  6. Ações participativas em uma comunidade virtual de enfermagem Acciones participativas en una comunidad virtual de enfemería Participating actions in a nursing virtual community

    Directory of Open Access Journals (Sweden)

    Jaqueline Santos de Andrade Martins

    2009-03-01

    Full Text Available O estudo aborda a interação no âmbito da Enfermagem em ambientes virtuais, ressaltando sua importância para o cuidado e aprendizagem da profissão. Objetivos: Propor uma categorização de ações participativas expressas em mensagens de identidades virtuais de uma comunidade de enfermagem; e verificar a distribuição das categorias de ações participativas, de modo a correlacioná-las com o processo de interação na comunidade virtual. Métodos: Estudo exploratório de abordagem quantitativa, com análise de conteúdo textual e estatística descritiva. Verificaram-se nove categorias para as ações participativas. Resultados: As categorias para as ações participativas mais prevalentes foram comentário, questionamento e resposta, sendo denominadas tríade da conversação. Conclusão: Pontua-se a validade em tomar as ações participativas como elementos básicos indicativos de interações e transações na perspectiva sistêmica aplicada à Enfermagem. Esta compreensão traz contribuições para uma melhor caracterização de ambientes, indivíduos e suas relações no espaço virtual da Internet.Este estudio se ocupa de la interacción de la enfermería en ambientes virtuales y su importancia para la atención y el aprendizaje de la enfermería. Objetivos: Proponer una clasificación de las cuotas de participación que se expresa en los mensajes de participación en una comunidad virtual de la enfermería, y comprobar la distribución de las categorías de acciones participativas con el fin de caracterizar la interacción en la comunidad virtual. Métodos: Estudio exploratorio de enfoque cuantitativo, con análise del contenido y con estadísticas descriptivas. Resultados: Las nueve categorías más prevalentes para el tipo de participación fueron comentarios, preguntas y respuestas que se llamaron "tríada de la conversación". Conclusión: Hay una validad del acciones participativas en la interacción y transición. Este

  7. A synthetic TLR4 agonist formulated in an emulsion enhances humoral and Type 1 cellular immune responses against GMZ2 - A GLURP-MSP3 fusion protein malaria vaccine candidate

    DEFF Research Database (Denmark)

    Lousada-Dietrich, Susana; Jogdand, Prajakta S; Jepsen, Søren;

    2011-01-01

    GMZ2 adjuvanted by aluminum hydroxide is a candidate malaria vaccine that has successfully passed phase 1 clinical testing in adult German and Gabonese volunteers and Gabonese children under five. Here we report a preclinical study screening a series of adjuvant vehicles and Toll-like receptor (TLR......) agonists in CB6F1 mice to identify an improved formulation of GMZ2 suitable for further human clinical studies. GMZ2 formulated in an oil-in-water emulsion plus the synthetic TLR4 agonist GLA elicits the highest (a) vaccine-specific IgG2a and total IgG titers, (b) parasite-specific IFA titers, (c) levels...... of Type 1 cytokine responses (IFN-¿), and (d) number of long-lived-plasma cells (LLPC) secreting antibodies against both the GMZ2 fusion and its two components. Thus, GLA helps to elicit a vaccine-specific Type 1 antibody profile together with high levels of LLPC, both of which are thought to be essential...

  8. Literature Review on Dynamic Cellular Manufacturing System

    Science.gov (United States)

    Nouri Houshyar, A.; Leman, Z.; Pakzad Moghadam, H.; Ariffin, M. K. A. M.; Ismail, N.; Iranmanesh, H.

    2014-06-01

    In previous decades, manufacturers faced a lot of challenges because of globalization and high competition in markets. These problems arise from shortening product life cycle, rapid variation in demand of products, and also rapid changes in manufcaturing technologies. Nowadays most manufacturing companies expend considerable attention for improving flexibility and responsiveness in order to overcome these kinds of problems and also meet customer's needs. By considering the trend toward the shorter product life cycle, the manufacturing environment is towards manufacturing a wide variety of parts in small batches [1]. One of the major techniques which are applied for improving manufacturing competitiveness is Cellular Manufacturing System (CMS). CMS is type of manufacturing system which tries to combine flexibility of job shop and also productivity of flow shop. In addition, Dynamic cellular manufacturing system which considers different time periods for the manufacturing system becomes an important topic and attracts a lot of attention to itself. Therefore, this paper made attempt to have a brief review on this issue and focused on all published paper on this subject. Although, this topic gains a lot of attention to itself during these years, none of previous researchers focused on reviewing the literature of that which can be helpful and useful for other researchers who intend to do the research on this topic. Therefore, this paper is the first study which has focused and reviewed the literature of dynamic cellular manufacturing system.

  9. Pirin inhibits cellular senescence in melanocytic cells.

    Science.gov (United States)

    Licciulli, Silvia; Luise, Chiara; Scafetta, Gaia; Capra, Maria; Giardina, Giuseppina; Nuciforo, Paolo; Bosari, Silvano; Viale, Giuseppe; Mazzarol, Giovanni; Tonelli, Chiara; Lanfrancone, Luisa; Alcalay, Myriam

    2011-05-01

    Cellular senescence has been widely recognized as a tumor suppressing mechanism that acts as a barrier to cancer development after oncogenic stimuli. A prominent in vivo model of the senescence barrier is represented by nevi, which are composed of melanocytes that, after an initial phase of proliferation induced by activated oncogenes (most commonly BRAF), are blocked in a state of cellular senescence. Transformation to melanoma occurs when genes involved in controlling senescence are mutated or silenced and cells reacquire the capacity to proliferate. Pirin (PIR) is a highly conserved nuclear protein that likely functions as a transcriptional regulator whose expression levels are altered in different types of tumors. We analyzed the expression pattern of PIR in adult human tissues and found that it is expressed in melanocytes and has a complex pattern of regulation in nevi and melanoma: it is rarely detected in mature nevi, but is expressed at high levels in a subset of melanomas. Loss of function and overexpression experiments in normal and transformed melanocytic cells revealed that PIR is involved in the negative control of cellular senescence and that its expression is necessary to overcome the senescence barrier. Our results suggest that PIR may have a relevant role in melanoma progression. PMID:21514450

  10. Effect of cellular mobility on immune response

    Science.gov (United States)

    Pandey, R. B.; Mannion, R.; Ruskin, H. J.

    2000-08-01

    Mobility of cell types in our HIV immune response model is subject to an intrinsic mobility and an explicit directed mobility, which is governed by Pmob. We investigate how restricting the explicit mobility, while maintaining the innate mobility of a viral-infected cell, affects the model's results. We find that increasing the explicit mobility of the immune system cells leads to viral dominance for certain levels of viral mutation. We conclude that increasing immune system cellular mobility indirectly increases the virus’ inherent mobility.

  11. Cellular spaces, null spaces and homotopy localization

    CERN Document Server

    Farjoun, Emmanuel Dror

    1996-01-01

    In this monograph we give an exposition of some recent development in homotopy theory. It relates to advances in periodicity in homotopy localization and in cellular spaces. The notion of homotopy localization is treated quite generally and encompasses all the known idempotent homotopy functors. It is applied to K-theory localizations, to Morava-theories, to Hopkins-Smith theory of types. The method of homotopy colimits is used heavily. It is written with an advanced graduate student in topology and research homotopy theorist in mind.

  12. Hierarchical Cellular Structures in High-Capacity Cellular Communication Systems

    CERN Document Server

    Jain, R K; Agrawal, N K

    2011-01-01

    In the prevailing cellular environment, it is important to provide the resources for the fluctuating traffic demand exactly in the place and at the time where and when they are needed. In this paper, we explored the ability of hierarchical cellular structures with inter layer reuse to increase the capacity of mobile communication network by applying total frequency hopping (T-FH) and adaptive frequency allocation (AFA) as a strategy to reuse the macro and micro cell resources without frequency planning in indoor pico cells [11]. The practical aspects for designing macro- micro cellular overlays in the existing big urban areas are also explained [4]. Femto cells are inducted in macro / micro / pico cells hierarchical structure to achieve the required QoS cost effectively.

  13. Differential effects of fenofibrate versus atorvastatin on the concentrations of E-selectin and vascular cellular adhesion molecule-1 in patients with type 2 diabetes mellitus and mixed hyperlipoproteinemia: a randomized cross-over trial

    Directory of Open Access Journals (Sweden)

    Otto Carsten

    2003-12-01

    Full Text Available Abstract Background Diabetic dyslipoproteinemia is characterized by hypertriglyceridemia, low HDL-cholesterol and often elevated LDL-cholesterol and is a strong risk factor for atherosclerosis. Adhesion molecule levels are elevated both in hyperlipoproteinemia and diabetes mellitus. It is unclear whether fibrate or statin therapy has more beneficial effects on adhesion molecule concentrations. Methods Atorvastatin (10 mg/d was compared to fenofibrate (200 mg/d each for 6 weeks separated by a 6 week washout period in 11 patients (6 male, 5 female; 61.8 ± 8.2 years; body mass index 29.8 ± 3.1 kg/m2 with type 2 diabetes mellitus (HbA1c 7.3 ± 1.1 % and mixed hyperlipoproteinemia using a randomized, cross-over design. Fasting blood glucose, HbA1c, lipid parameters, E-selectin, ICAM-1, VCAM-1, and fibrinogen concentrations were determined before and after each drug. Results Glucose and HbA1c concentrations remained unchanged during the whole study period. LDL cholesterol was reduced during atorvastatin therapy, triglycerides were lowered more effectively with fenofibrate. Comparison of pre- and postreatment concentrations of E-selectin showed a reduction during atorvastatin (-7 %, p = 0.11 and fenofibrate (-10 %, p Conclusions In addition to the different beneficial effects on lipid metabolism, both drugs appear to lower adhesion molecule plasma concentrations in a different manner in patients with type 2 diabetes and mixed hyperlipoproteinemia. Our observations should be confirmed in a larger cohort of such patients.

  14. Continuum representations of cellular solids

    Energy Technology Data Exchange (ETDEWEB)

    Neilsen, M.K.

    1993-09-01

    Cellular materials consist of interconnected struts or plates which form cells. The struts or plates are constructed from a variety of metals, polymers, ceramics and wood products. Cellular materials are often used in impact limiters for shipping containers to protect the contents from accidental impact events. These materials exhibit a variety of complex behavior when subjected to crushing loads. This research focuses on the development of continuum representations of cellular solids that can be used in the finite element analysis of shipping container accidents. A significant portion of this work is the development of a new methodology to relate localized deformations to appropriate constitutive descriptions. This methodology provides the insight needed to select constitutive descriptions for cellular solids that capture the localized deformations that are observed experimentally. Constitutive relations are developed for two different cellular materials, aluminum honeycomb and polyurethane foam. These constitutive relations are based on plasticity and continuum damage theories. Plasticity is used to describe the permanent deformation exhibited by both aluminum honeycomb and polyurethane foam. Continuum damage is needed to capture the change in elastic parameters due to cracking of the polyurethane cell wall materials. The new constitutive description of polyurethane foam is implemented in both static and dynamic finite element codes, and analytical and numerical predictions are compared with available experimental data.

  15. Prognosis of Different Cellular Generations

    Directory of Open Access Journals (Sweden)

    Preetish Ranjan

    2013-04-01

    Full Text Available Technological advancement in mobile telephony from 1G to 3G, 4G and 5G has a very axiomatic fact that made an entire world a global village. The cellular system employs a different design approach and technology that most commercial radio and television system use. In the cellular system, the service area is divided into cells and a transmitter is designed to serve an individual cell. The system seeks to make efficient use of available channels by using low-power transmitters to allow frequency reuse at a smaller distance. Maximizing the number of times each channel can be reused in a given geographical area is the key to an efficient cellular system design. During the past three decades, the world has seen significant changes in telecommunications industry. There have been some remarkable aspects to the rapid growth in wireless communications, as seen by the large expansion in mobile systems. This paper focuses on “Past, Present & Future of Cellular Telephony” and some light has been thrown upon the technologies of the cellular systems, namely 1G, 2G, 2.5G, 3G and future generations like 4G and 5G systems as well.

  16. Aging, cellular senescence, and cancer.

    Science.gov (United States)

    Campisi, Judith

    2013-01-01

    For most species, aging promotes a host of degenerative pathologies that are characterized by debilitating losses of tissue or cellular function. However, especially among vertebrates, aging also promotes hyperplastic pathologies, the most deadly of which is cancer. In contrast to the loss of function that characterizes degenerating cells and tissues, malignant (cancerous) cells must acquire new (albeit aberrant) functions that allow them to develop into a lethal tumor. This review discusses the idea that, despite seemingly opposite characteristics, the degenerative and hyperplastic pathologies of aging are at least partly linked by a common biological phenomenon: a cellular stress response known as cellular senescence. The senescence response is widely recognized as a potent tumor suppressive mechanism. However, recent evidence strengthens the idea that it also drives both degenerative and hyperplastic pathologies, most likely by promoting chronic inflammation. Thus, the senescence response may be the result of antagonistically pleiotropic gene action. PMID:23140366

  17. Membrane potential mediates the cellular binding of nanoparticles

    Science.gov (United States)

    Shin, Edwin H.; Li, Ye; Kumar, Umesh; Sureka, Hursh V.; Zhang, Xianren; Payne, Christine K.

    2013-06-01

    The use of nanoparticles for cellular therapeutic or sensing applications requires nanoparticles to bind, or adhere, to the cell surface. While nanoparticle parameters such as size, shape, charge, and composition are important factors in cellular binding, the cell itself must also be considered. All cells have an electrical potential across the plasma membrane driven by an ion gradient. Under standard conditions the ion gradient will result in a -10 to -100 mV potential across the membrane with a net negative charge on the cytosolic face. Using a combination of flow cytometry and fluorescence microscopy experiments and dissipative particle dynamics simulations, we have found that a decrease in membrane potential leads to decreased cellular binding of anionic nanoparticles. The decreased cellular binding of anionic nanoparticles is a general phenomenon, independent of depolarization method, nanoparticle composition, and cell type. Increased membrane potential reverses this trend resulting in increased binding of anionic nanoparticles. The cellular binding of cationic nanoparticles is minimally affected by membrane potential due to the interaction of cationic nanoparticles with cell surface proteins. The influence of membrane potential on the cellular binding of nanoparticles is especially important when considering the use of nanoparticles in the treatment or detection of diseases, such as cancer, in which the membrane potential is decreased.The use of nanoparticles for cellular therapeutic or sensing applications requires nanoparticles to bind, or adhere, to the cell surface. While nanoparticle parameters such as size, shape, charge, and composition are important factors in cellular binding, the cell itself must also be considered. All cells have an electrical potential across the plasma membrane driven by an ion gradient. Under standard conditions the ion gradient will result in a -10 to -100 mV potential across the membrane with a net negative charge on the

  18. Adaptive stochastic cellular automata: Applications

    Science.gov (United States)

    Qian, S.; Lee, Y. C.; Jones, R. D.; Barnes, C. W.; Flake, G. W.; O'Rourke, M. K.; Lee, K.; Chen, H. H.; Sun, G. Z.; Zhang, Y. Q.; Chen, D.; Giles, C. L.

    1990-09-01

    The stochastic learning cellular automata model has been applied to the problem of controlling unstable systems. Two example unstable systems studied are controlled by an adaptive stochastic cellular automata algorithm with an adaptive critic. The reinforcement learning algorithm and the architecture of the stochastic CA controller are presented. Learning to balance a single pole is discussed in detail. Balancing an inverted double pendulum highlights the power of the stochastic CA approach. The stochastic CA model is compared to conventional adaptive control and artificial neural network approaches.

  19. Cellular automaton for chimera states

    Science.gov (United States)

    García-Morales, Vladimir

    2016-04-01

    A minimalistic model for chimera states is presented. The model is a cellular automaton (CA) which depends on only one adjustable parameter, the range of the nonlocal coupling, and is built from elementary cellular automata and the majority (voting) rule. This suggests the universality of chimera-like behavior from a new point of view: Already simple CA rules based on the majority rule exhibit this behavior. After a short transient, we find chimera states for arbitrary initial conditions, the system spontaneously splitting into stable domains separated by static boundaries, some synchronously oscillating and the others incoherent. When the coupling range is local, nontrivial coherent structures with different periodicities are formed.

  20. Cellular senescence in aging primates.

    Science.gov (United States)

    Herbig, Utz; Ferreira, Mark; Condel, Laura; Carey, Dee; Sedivy, John M

    2006-03-01

    The aging of organisms is characterized by a gradual functional decline of all organ systems. Mammalian somatic cells in culture display a limited proliferative life span, at the end of which they undergo an irreversible cell cycle arrest known as replicative senescence. Whether cellular senescence contributes to organismal aging has been controversial. We investigated telomere dysfunction, a recently discovered biomarker of cellular senescence, and found that the number of senescent fibroblasts increases exponentially in the skin of aging baboons, reaching >15% of all cells in very old individuals. In addition, the same cells contain activated ataxia-telangiectasia mutated kinase and heterochromatinized nuclei, confirming their senescent status. PMID:16456035

  1. Cellular membrane trafficking of mesoporous silica nanoparticles

    Energy Technology Data Exchange (ETDEWEB)

    Fang, I-Ju [Iowa State Univ., Ames, IA (United States)

    2012-01-01

    This dissertation mainly focuses on the investigation of the cellular membrane trafficking of mesoporous silica nanoparticles. We are interested in the study of endocytosis and exocytosis behaviors of mesoporous silica nanoparticles with desired surface functionality. The relationship between mesoporous silica nanoparticles and membrane trafficking of cells, either cancerous cells or normal cells was examined. Since mesoporous silica nanoparticles were applied in many drug delivery cases, the endocytotic efficiency of mesoporous silica nanoparticles needs to be investigated in more details in order to design the cellular drug delivery system in the controlled way. It is well known that cells can engulf some molecules outside of the cells through a receptor-ligand associated endocytosis. We are interested to determine if those biomolecules binding to cell surface receptors can be utilized on mesoporous silica nanoparticle materials to improve the uptake efficiency or govern the mechanism of endocytosis of mesoporous silica nanoparticles. Arginine-glycine-aspartate (RGD) is a small peptide recognized by cell integrin receptors and it was reported that avidin internalization was highly promoted by tumor lectin. Both RGD and avidin were linked to the surface of mesoporous silica nanoparticle materials to investigate the effect of receptor-associated biomolecule on cellular endocytosis efficiency. The effect of ligand types, ligand conformation and ligand density were discussed in Chapter 2 and 3. Furthermore, the exocytosis of mesoporous silica nanoparticles is very attractive for biological applications. The cellular protein sequestration study of mesoporous silica nanoparticles was examined for further information of the intracellular pathway of endocytosed mesoporous silica nanoparticle materials. The surface functionality of mesoporous silica nanoparticle materials demonstrated selectivity among the materials and cancer and normal cell lines. We aimed to determine

  2. Repaglinide at a cellular level

    DEFF Research Database (Denmark)

    Krogsgaard Thomsen, M; Bokvist, K; Høy, M;

    2002-01-01

    To investigate the hormonal and cellular selectivity of the prandial glucose regulators, we have undertaken a series of experiments, in which we characterised the effects of repaglinide and nateglinide on ATP-sensitive potassium ion (KATP) channel activity, membrane potential and exocytosis in ra...

  3. Analysis of cellular manufacturing systems

    NARCIS (Netherlands)

    Heragu, Sunderesh; Meng, Gang; Zijm, Henk; Ommeren, van Jan-Kees

    2001-01-01

    In this paper, we present an open queuing network modeling approach to estimate performance measures of a cellular manufacturing layout. It is assumed a layout and production data for a planning period of specified length are available. The production data takes into account, processing and handli

  4. Cellular uptake of metallated cobalamins

    DEFF Research Database (Denmark)

    Tran, MQT; Stürup, Stefan; Lambert, Ian H.;

    2016-01-01

    Cellular uptake of vitamin B12-cisplatin conjugates was estimated via detection of their metal constituents (Co, Pt, and Re) by inductively coupled plasma mass spectrometry (ICP-MS). Vitamin B12 (cyano-cob(iii)alamin) and aquo-cob(iii)alamin [Cbl-OH2](+), which differ in the β-axial ligands (CN(-...

  5. Acciones educativas dirigidas al personal médico y de enfermería para disminuir las infecciones del tracto sanguíneo relacionadas a catéteres venosos centrales

    Directory of Open Access Journals (Sweden)

    Milena Villegas Sánchez

    2014-10-01

    Full Text Available Introducción. La bacteriemia relacionada a Catéter Venoso  Central  es una de las principales complicaciones en los pacientes a quienes se les instala un catéter intravascular. Las acciones educativas han mostrado disminución en la tasa de estas infecciones en diferentes estudios.  El objetivo fue determinar la tasa de Infecciones del tracto sanguíneo relacionadas al catéter venoso central (ITS/CVC, en el Servicio de Cuidado Intensivo Neonatal, implementar las acciones preventivas y cuantificar el impacto.Metodología. Es un estudio descriptivo en donde se compara la tasa de ITS/CVC, antes y después de una intervención educativa, en  los pacientes ingresados a SECIN.  La tasa de ITS/CVC  se obtuvo dividiendo el número de éstas entre el número de días-catéter y se multiplicó por 1 000.Resultado. Un resultado relevante es que en el periodo  pre intervención se observa una  tasa  de 32,84 infecciones por 1 000 días (cuarto trimestre del 2012 y primer trimestre del 2013  y en el periodo post intervención la tasa fue de 8,28 infecciones por 1 000 días. (Tercer trimestre y cuarto trimestre del 2013.Conclusión. Se concluye que las acciones educativas para disminuir la tasa de ITS/CVC fueron efectivas.

  6. Terapias, prácticas y acciones en la búsqueda de un mejor estado de salud-enfermedad Therapies, practices and actions in the search for better health

    OpenAIRE

    Mercedes Arévalo de Andrade; Nien Tzu Weng Huang; Luis Jiménez Herrera

    2011-01-01

    Alrededor del mundo y a lo largo de la historia, las personas han realizad y seguirán realizando actividades, acciones, prácticas y terapias, en su afán de contar con un mejor estado de salud enfermedad. Materiales y Métodos: Se buscaron artículos científicos principalmente en las bases de datos disponibles en el Sistema de Bibliotecas y Documentación de la Universidad de Costa Rica, además de otras fuentes de información, luego del análisis correspondiente se estructuró una comunicación que ...

  7. Acciones de enfermería para la prevención de la neumonía asociada a ventilación mecánica: revisión sistemática

    OpenAIRE

    Kluczynik Vieira, Caroline Evelin Nascimento; de Andrade, Paula Stefânia; Enders, Bertha Cruz; Coura, Alexsandro Silva; Dutra, Michelinne Oliveira Machado

    2014-01-01

    Objetivo: Identificar acciones de enfermería implementadas en la prevención de la neumonía asociada a ventilación mecánica en los pacientes intubados en la Unidad de Cuidados Intensivos. Método: Revisión sistemática en las bases: PubMed, BVS y Scielo utilizando los descriptores: "Atención de Enfermería" y "Neumonía Asociada al Ventilador". La muestra constaba de 13 artículos. La calidad interna se evaluó mediante la clasificación de Jadad. Resultados: Los destaqu...

  8. Estudio integral del análisis de la calidad y seguridad alimentaria con el fin de definir acciones estratégicas por parte de la industria agroalimentaria

    OpenAIRE

    Carlos Villellas, María Pilar de

    2007-01-01

    Resumen Esta Tesis, titulada ‘Estudio integral del análisis de la calidad y seguridad alimentaria con el fin de definir acciones estratégicas por parte de la industria agroalimentaria' propone el estudio de la calidad y seguridad alimentaria desde una perspectiva doble, por una parte, la perspectiva del consumidor a través de sus percepciones y preferencias en el momento de realizar la compra y por otra parte, la perspectiva de la industria mediante la implementación de una metodología co...

  9. La política social frente a las familias víctimas de la violencia delincuencial vinculada al narcotráfico: evidenciar las necesidades y articular las acciones

    OpenAIRE

    Salazar Serna, Karla; Ribeiro Ferreira, Manuel; Mendoza Cárdenas, Héctor A.

    2015-01-01

    El presente artículo pretende profundizar en la discusión sobre la necesidad apremiante de diseñar e implementar políticas sociales de atención a las familias víctimas de la violencia en México; para ello, se realiza un análisis que vincula posicionamientos teóricos y bases jurídicas nacionales e internacionales, el cual fundamenta la acción obligada del Estado Mexicano para establecer acciones gubernamentales que atiendan los diversos problemas generados a partir de esta vi...

  10. Probing cellular behaviors through nanopatterned chitosan membranes

    International Nuclear Information System (INIS)

    This paper describes a high-throughput method for developing physically modified chitosan membranes to probe the cellular behavior of MDCK epithelial cells and HIG-82 fibroblasts adhered onto these modified membranes. To prepare chitosan membranes with micro/nanoscaled features, we have demonstrated an easy-to-handle, facile approach that could be easily integrated with IC-based manufacturing processes with mass production potential. These physically modified chitosan membranes were observed by scanning electron microscopy to gain a better understanding of chitosan membrane surface morphology. After MDCK cells and HIG-82 fibroblasts were cultured on these modified chitosan membranes for various culture durations (i.e. 1, 2, 4, 12 and 24 h), they were investigated to decipher cellular behavior. We found that both cells preferred to adhere onto a flat surface rather than on a nanopatterned surface. However, most (> 80%) of the MDCK cells showed rounded morphology and would suspend in the cultured medium instead of adhering onto the planar surface of negatively nanopatterned chitosan membranes. This means different cell types (e.g. fibroblasts versus epithelia) showed distinct capabilities/preferences of adherence for materials of varying surface roughness. We also showed that chitosan membranes could be re-used at least nine times without significant contamination and would provide us consistency for probing cell–material interactions by permitting reuse of the same substrate. We believe these results would provide us better insight into cellular behavior, specifically, microscopic properties and characteristics of cells grown under unique, nanopatterned cell-interface conditions. (paper)

  11. CELLULAR INTERACTIONS MEDIATED BY GLYCONECTIDS

    Directory of Open Access Journals (Sweden)

    O.Popescu

    1999-01-01

    Full Text Available Cellular interactions involve many types of cell surface molecules and operate via homophilic and/or heterophilic protein-protein and protein-carbohydrate binding. Our investigations in different model-systems (marine invertebrates and mammals have provided direct evidence that a novel class of primordial proteoglycans, named by us gliconectins, can mediate cell adhesion via a new alternative molecular mechanism of polyvalent carbohydrate-carbohydrate binding. Biochemical characterization of isolated and purified glyconectins revealed the presence of specific carbohydrate structures, acidic glycans, different from classical glycosaminoglycans. Such acidic glycans of high molecular weight containing fucose, glucuronic or galacturonic acids, and sulfate groups, originally found in sponges and sea urchin embryos, may represent a new class of carbohydrate carcino-embryonal antigens in mice and humans. Such interactions between biological macromolecules are usually investigated by kinetic binding studies, calorimetric methods, X-ray diffraction, nuclear magnetic resonance, and other spectroscopic analyses. However, these methods do not supply a direct estimation of the intermolecular binding forces that are fundamental for the function of the ligand-receptor association. Recently, we have introduced atomic force microscopy to quantify the binding strength between cell adhesion proteoglycans. Measurement of binding forces intrinsic to cell adhesion proteoglycans is necessary to assess their contribution to the maintenance of the anatomical integrity of multicellular organisms. As a model, we selected the glyconectin 1, a cell adhesion proteoglycan isolated from the marine sponge Microciona prolifera. This glyconectin mediates in vivo cell recognition and aggregation via homophilic, species-specific, polyvalent, and calcium ion-dependent carbohydrate-carbohydrate interactions. Under physiological conditions, an adhesive force of up to 400 piconewtons

  12. Modeling In Vitro Cellular Responses to Silver Nanoparticles

    Directory of Open Access Journals (Sweden)

    Dwaipayan Mukherjee

    2014-01-01

    Full Text Available Engineered nanoparticles (NPs have been widely demonstrated to induce toxic effects to various cell types. In vitro cell exposure systems have high potential for reliable, high throughput screening of nanoparticle toxicity, allowing focusing on particular pathways while excluding unwanted effects due to other cells or tissue dosimetry. The work presented here involves a detailed biologically based computational model of cellular interactions with NPs; it utilizes measurements performed in human cell culture systems in vitro, to develop a mechanistic mathematical model that can support analysis and prediction of in vivo effects of NPs. The model considers basic cellular mechanisms including proliferation, apoptosis, and production of cytokines in response to NPs. This new model is implemented for macrophages and parameterized using in vitro measurements of changes in cellular viability and mRNA levels of cytokines: TNF, IL-1b, IL-6, IL-8, and IL-10. The model includes in vitro cellular dosimetry due to nanoparticle transport and transformation. Furthermore, the model developed here optimizes the essential cellular parameters based on in vitro measurements, and provides a “stepping stone” for the development of more advanced in vivo models that will incorporate additional cellular and NP interactions.

  13. Cellular solidification of transparent monotectics

    Science.gov (United States)

    Kaulker, W. F.

    1986-01-01

    Understanding how liquid phase particles are engulfed or pushed during freezing of a monotectic is addressed. The additional complication is that the solid-liquid interface is nonplanar due to constitutional undercooling. Some evidence of particle pushing where the particles are the liquid phase of the montectic was already observed. Cellular freezing of the succinonitrile-glycerol system also occurred. Only a few compositions were tested at that time. The starting materials were not especially pure so that cellular interface observed was likely due to the presence of unkown impurities, the major portion of which was water. Topics addressed include: the effort of modeling the particle pushing process using the computer, establishing an apparatus for the determination of phase diagrams, and the measurement of the temperature gradients with a specimen which will solidify on the temperature gradient microscope stage.

  14. Jeans type instability for a chemotactic model of cellular aggregation

    CERN Document Server

    Chavanis, Pierre-Henri

    2008-01-01

    We consider an inertial model of chemotactic aggregation generalizing the Keller-Segel model and we study the linear dynamical stability of an infinite and homogeneous distribution of cells (bacteria, amoebae, endothelial cells,...) when inertial effects are accounted for. These inertial terms model cells directional persistance. We determine the condition of instability and the growth rate of the perturbation as a function of the cell density and the wavelength of the perturbation. We discuss the differences between overdamped (Keller-Segel) and inertial models. Finally, we show the analogy between the instability criterion for biological populations and the Jeans instability criterion in astrophysics.

  15. Analysis of cellular manufacturing systems

    OpenAIRE

    Heragu, Sunderesh; Meng, Gang; Zijm, Henk; Ommeren, van, J.C.

    2001-01-01

    In this paper, we present an open queuing network modeling approach to estimate performance measures of a cellular manufacturing layout. It is assumed a layout and production data for a planning period of specified length are available. The production data takes into account, processing and handling set-up times as well as transfer and process batch size information of multiple products that flow through the system. It is assumed that two sets of discrete material handling devices are used fo...

  16. Identification of Nonstationary Cellular Automata

    Institute of Scientific and Technical Information of China (English)

    AndrewI.Adamatzky

    1992-01-01

    The principal feature of nonstationary cellular automata(NCA) is that a local transitiol rule of each cell is changed at each time step depending on neighborhood configuration at previous time step.The identification problem for NCA is extraction of local transition rules and the establishment of mechanism for changing these rules using sequence of NCA configurations.We present serial and parallel algorithms for identification of NCA.

  17. The Origins of Cellular Life

    OpenAIRE

    Schrum, Jason P.; Zhu, Ting F.; SZOSTAK, JACK W.

    2010-01-01

    Understanding the origin of cellular life on Earth requires the discovery of plausible pathways for the transition from complex prebiotic chemistry to simple biology, defined as the emergence of chemical assemblies capable of Darwinian evolution. We have proposed that a simple primitive cell, or protocell, would consist of two key components: a protocell membrane that defines a spatially localized compartment, and an informational polymer that allows for the replication and inheritance of fun...

  18. Stochastic Nature in Cellular Processes

    Institute of Scientific and Technical Information of China (English)

    刘波; 刘圣君; 王祺; 晏世伟; 耿轶钊; SAKATA Fumihiko; GAO Xing-Fa

    2011-01-01

    The importance of stochasticity in cellular processes is increasingly recognized in both theoretical and experimental studies. General features of stochasticity in gene regulation and expression are briefly reviewed in this article, which include the main experimental phenomena, classification, quantization and regulation of noises. The correlation and transmission of noise in cascade networks are analyzed further and the stochastic simulation methods that can capture effects of intrinsic and extrinsic noise are described.

  19. CELLULAR FETAL MICROCHIMERISM IN PREECLAMPSIA

    OpenAIRE

    Gammill, Hilary S; Aydelotte, Tessa M.; Guthrie, Katherine A.; Nkwopara, Evangelyn C.; Nelson, J. Lee

    2013-01-01

    Previous studies have shown elevated concentrations of free fetal deoxyribonucleic acid and erythroblasts in maternal circulation in preeclampsia compared with normal pregnancy. Pluripotent and immunocompetent fetal cells also transfer to the maternal circulation during pregnancy, but whether concentrations of fetal mononuclear cells also differed in preeclampsia was unknown. We sought to quantify cellular fetal microchimerism in maternal circulation in women with preeclampsia and healthy con...

  20. Cellular reactions to patterned biointerfaces

    OpenAIRE

    Schulte, Vera Antonie

    2012-01-01

    The subject of this thesis is to study cellular reactions to topographically, mechanically and biochemically tunable polymeric biomaterials. Different aspects of in vitro cell-biomaterial interactions were systematically studied with the murine fibroblast cell line NIH L929 and primary human dermal fibroblasts (HDFs). Besides a general cytocompatibility assessment of the applied materials and the quantification of cell adhesion per se, cell morphological changes (e.g. cell spreading) and intr...

  1. Cellular communications a comprehensive and practical guide

    CERN Document Server

    Tripathi, Nishith

    2014-01-01

    Even as newer cellular technologies and standards emerge, many of the fundamental principles and the components of the cellular network remain the same. Presenting a simple yet comprehensive view of cellular communications technologies, Cellular Communications provides an end-to-end perspective of cellular operations, ranging from physical layer details to call set-up and from the radio network to the core network. This self-contained source forpractitioners and students represents a comprehensive survey of the fundamentals of cellular communications and the landscape of commercially deployed

  2. Eukaryotic protein domains as functional units of cellular evolution

    DEFF Research Database (Denmark)

    Jin, Jing; Xie, Xueying; Chen, Chen;

    2009-01-01

    domain compositions and functional properties, termed "domain clubs," which we use to compare multiple eukaryotic proteomes. This analysis shows that different domain types can take distinct evolutionary trajectories, which correlate with the conservation, gain, expansion, or decay of particular...... of different domain types to assess the molecular compartment occupied by each domain. This reveals that specific subsets of domains demarcate particular cellular processes, such as growth factor signaling, chromatin remodeling, apoptotic and inflammatory responses, or vesicular trafficking. We suggest...

  3. La coopetencia y las acciones afirmativas como una propuesta para mejorar el proceso de integración en la CAN

    Directory of Open Access Journals (Sweden)

    Mario Andrés Pinzón Camargo

    2008-08-01

    Full Text Available El análisis económico del derecho ofrece un conjunto de herramientas, entre las que se puede mencionar la teoría de juegos, con las cuales se persigue la generación de escenarios de análisis donde sea posible estudiar la conducta del individuo ante un marco normativo. en este trabajo, el uso de dichas herramientas se ha orientado a explicar el comportamiento de los estados, entendidos como agentes o jugadores de un proceso de integración subregional (Comunidad andina de naciones-can, en el que deben decidir las estrategias a seguir con el propósito de promover e impulsar avances significativos en la Comunidad, sobre la base de una estructura de incentivos; en este contexto, y atendiendo los supuestos del aed, estos agentes son definidos como racionales, egoístas y maximizadores, lo cual se adecúa a los supuestos que hace la teoría de juegos respecto de los jugadores involucrados en situaciones de interacción estratégica modeladas como juegos. En este escenario, al realizar un estudio de la estructura de estímulos tanto generales como preferenciales para Bolivia y ecuador, ideada por el Acuerdo de Cartagena para fomentar la integración en el caso de la liberalización del comercio de servicios, se observa que aquella no ha sido la más adecuada, por cuanto no ha conducido a la obtención de los resultados esperados. Así las cosas, este documento propone un nueva estructura de incentivos sobre la base de la construcción de un ‘juego coopetitivo’, definido como aquel que resulta de una adecuada complementación entre un estadio inicial de cooperación, seguido por uno de competencia. como resultado de esta construcción para el caso de la can, se ha modelado, de una parte, una función recompensa generada a partir de unas funciones de subvención y sanción, a modo de incentivo, y de otra, un juego cooperativo que adopta y adapta el concepto de acciones afirmativas. La relación entre lo obtenido por cada jugador en el juego

  4. Acciones educativas para favorecer la educación ambiental a través del programa audiovisual en escolares del Taller Técnica Básica Agropecuaria.

    Directory of Open Access Journals (Sweden)

    Sonia Mareidy González Migueles

    2009-03-01

    El trabajo tiene como objetivo aplicar acciones educativas para favorecer la Educación Ambiental a través del Programa Audiovisual en escolares del taller Técnica Básica Agropecuaria. Las acciones educativas propuestas como solución resultaron muy efectivas, ya que los escolares fueron modelando su actuación en correspondencia con los conocimientos asimilados sobre la problemática abordada, por lo que se logró favorecer su Educación Ambiental paulatinamente. Se consideran valiosos los resultados ya que han permitido elevar el nivel de conocimiento de los educandos, al adquirir conceptos medio ambientales, reconocer y valorar actuaciones beneficiosas y perjudiciales al hombre porque provocan deterioro y ha favorecido el grado de motivación por su cuidado manifestado en el nivel de participación y el grado de satisfacción de la misma. La utilización del Programa Audiovisual propicia el desarrollo de la Educación Ambiental y la preparación laboral del alumno con necesidades educativas especiales de una forma amena, elevando el nivel de conocimiento de los escolares preparándolos con mayor eficiencia para una vida adulta e independiente.

  5. Particles and Patterns in Cellular Automata

    International Nuclear Information System (INIS)

    This is the final report of a three-year, Laboratory Directed Research and Development (LDRD) project at Los Alamos National Laboratory (LANL). Our objective has been to develop tools for studying particle interactions in a class of dynamical systems characterized by discreteness, determinism, local interaction, and an inherently parallel form of evolution. These systems can be described by cellular automata (CA) and the behavior we studied has improved our understanding of the nature of patterns generated by CAs, their ability to perform global computations, and their relationship to continuous dynamical systems. We have also developed a rule-table mathematics that enables one to custom-design CA rule tables to generate patterns of specified types, or to perform specified computational tasks

  6. Mathematical analysis of complex cellular activity

    CERN Document Server

    Bertram, Richard; Teka, Wondimu; Vo, Theodore; Wechselberger, Martin; Kirk, Vivien; Sneyd, James

    2015-01-01

    This book contains two review articles on mathematical physiology that deal with closely related topics but were written and can be read independently. The first article reviews the basic theory of calcium oscillations (common to almost all cell types), including spatio-temporal behaviors such as waves. The second article uses, and expands on, much of this basic theory to show how the interaction of cytosolic calcium oscillators with membrane ion channels can result in highly complex patterns of electrical spiking. Through these examples one can see clearly how multiple oscillatory processes interact within a cell, and how mathematical methods can be used to understand such interactions better. The two reviews provide excellent examples of how mathematics and physiology can learn from each other, and work jointly towards a better understanding of complex cellular processes. Review 1: Richard Bertram, Joel Tabak, Wondimu Teka, Theodore Vo, Martin Wechselberger: Geometric Singular Perturbation Analysis of Burst...

  7. Cellular regulation of the dopamine transporter

    DEFF Research Database (Denmark)

    Eriksen, Jacob

    2010-01-01

    -membrane spanning protein Tac, thereby creating an extracellular antibody epitope. Upon expression in HEK293 cells this TacDAT fusion protein displayed functional properties similar to the wild type transporter. In an ELISA based internalization assay, TacDAT intracellular accumulation was increased by inhibitors......The dopamine transporter (DAT) mediates reuptake of dopamine from the synaptic cleft and is a target for widely abused psychostimulants such as cocaine and amphetamine. Nonetheless, little is known about the cellular distribution and trafficking of natively expressed DAT. DAT and its trafficking...... to natively expressed transporter, DAT was visualized directly in cultured DA neurons using the fluorescent cocaine analog JHC 1-64. These data showed pronounced colocalization upon constitutive internalization with Lysotracker, a late endosomal/lysosomal marker; however only little cololization was observed...

  8. [In vitro and in vivo study of electromagnetic compatibility of cellular phones and pacemakers].

    Science.gov (United States)

    Gellér, L; Thuróczy, G; Merkely, B

    2001-09-01

    Electromagnetic compatibility (EMC) of cellular phones and pacemakers (PM) was examined in four different cellular phone system (NMT, GSM, RLL, DCS 1800 MHz) and in fifteen different PM type in-vitro and in-vivo in humans. After more than 1100 in-vitro and 130 in-vivo tests we concluded, that the electromagnetic immunity of the PMs which are implanted in Hungary is suitable with only few exceptions. The highest rate of EMC problems was observed with NMT 450 MHz cellular phones (10.5%-63%). There was no EMC disturbance observed with GSM and DCS 1800 MHz cellular phones. There was only one case when clinically significant symptom was noticed with only one PM type and with NMT system cellular phone when the distance of cellular phone was 3-4 cms, and the power was maximal. There was not any EMC disturbance observed with none of the cellular phone systems during normal talking and when the distance of the PM and cellular phone was more than 20 cms. Our study supports guidelines which suggest that PM patients should contact their physicians when using cellular phones and cellular phones and PMs should not get closer than 20 cms.

  9. Estimation in Cellular Radio Systems

    OpenAIRE

    Blom, Jonas; Gunnarsson, Fredrik; Gustafsson, Fredrik

    1999-01-01

    The problem to track time-varying parameters in cellular radio systems is studied, and the focus is on estimation based only on the signals that are readily available. Previous work have demonstrated very good performance, but were relying on analog measurement that are not available. Most of the information is lost due to quantization and sampling at a rate that might be as low as 2 Hz (GSM case). For that matter a maximum likelihood estimator have been designed and exemplified in the case o...

  10. Cellular immune responses to HIV

    Science.gov (United States)

    McMichael, Andrew J.; Rowland-Jones, Sarah L.

    2001-04-01

    The cellular immune response to the human immunodeficiency virus, mediated by T lymphocytes, seems strong but fails to control the infection completely. In most virus infections, T cells either eliminate the virus or suppress it indefinitely as a harmless, persisting infection. But the human immunodeficiency virus undermines this control by infecting key immune cells, thereby impairing the response of both the infected CD4+ T cells and the uninfected CD8+ T cells. The failure of the latter to function efficiently facilitates the escape of virus from immune control and the collapse of the whole immune system.

  11. Cellular automata a parallel model

    CERN Document Server

    Mazoyer, J

    1999-01-01

    Cellular automata can be viewed both as computational models and modelling systems of real processes. This volume emphasises the first aspect. In articles written by leading researchers, sophisticated massive parallel algorithms (firing squad, life, Fischer's primes recognition) are treated. Their computational power and the specific complexity classes they determine are surveyed, while some recent results in relation to chaos from a new dynamic systems point of view are also presented. Audience: This book will be of interest to specialists of theoretical computer science and the parallelism challenge.

  12. Game of Life Cellular Automata

    CERN Document Server

    Adamatzky, Andrew

    2010-01-01

    In the late 1960s, British mathematician John Conway invented a virtual mathematical machine that operates on a two-dimensional array of square cell. Each cell takes two states, live and dead. The cells' states are updated simultaneously and in discrete time. A dead cell comes to life if it has exactly three live neighbours. A live cell remains alive if two or three of its neighbours are alive, otherwise the cell dies. Conway's Game of Life became the most programmed solitary game and the most known cellular automaton. The book brings together results of forty years of study into computational

  13. Protein accounting in the cellular economy

    Science.gov (United States)

    Vázquez-Laslop, Nora; Mankin, Alexander S.

    2014-01-01

    Knowing the copy number of cellular proteins is critical for understanding cell physiology. By being able to measure the absolute synthesis rates of the majority of cellular proteins, Li et al. (2014) gain insights into key aspects of translation regulation and fundamental principles of cellular strategies to adjust protein synthesis according to the needs. PMID:24766801

  14. Cellular identity at the single-cell level.

    Science.gov (United States)

    Coskun, Ahmet F; Eser, Umut; Islam, Saiful

    2016-10-20

    A single cell creates surprising heterogeneity in a multicellular organism. While every organismal cell shares almost an identical genome, molecular interactions in cells alter the use of DNA sequences to modulate the gene of interest for specialization of cellular functions. Each cell gains a unique identity through molecular coding across the DNA, RNA, and protein conversions. On the other hand, loss of cellular identity leads to critical diseases such as cancer. Most cell identity dissection studies are based on bulk molecular assays that mask differences in individual cells. To probe cell-to-cell variability in a population, we discuss single cell approaches to decode the genetic, epigenetic, transcriptional, and translational mechanisms for cell identity formation. In combination with molecular instructions, the physical principles behind cell identity determination are examined. Deciphering and reprogramming cellular types impact biology and medicine.

  15. In vivo cellular imaging using fluorescent proteins - Methods and Protocols

    Directory of Open Access Journals (Sweden)

    M. Monti

    2012-12-01

    Full Text Available The discovery and genetic engineering of fluorescent proteins has revolutionized cell biology. What was previously invisible to the cell often can be made visible with the use of fluorescent proteins. With this words, Robert M. Hoffman introduces In vivo Cellular Imaging Using Fluorescent proteins, the eighteen chapters book dedicated to the description of how fluorescence proteins have changed the way to analyze cellular processes in vivo. Modern researches aim to study new and less invasive methods able to follow the behavior of different cell types in different biological contexts: for example, how cancer cells migrate or how they respond to different therapies. Also, in vivo systems can help researchers to better understand animal embryonic development so as how fluorescence proteins may be used to monitor different processes in living organisms at the molecular and cellular level.

  16. Cellular uptake of metallated cobalamins.

    Science.gov (United States)

    Tran, Mai Thanh Quynh; Stürup, Stefan; Lambert, Ian Henry; Gammelgaard, Bente; Furger, Evelyne; Alberto, Roger

    2016-03-01

    Cellular uptake of vitamin B12-cisplatin conjugates was estimated via detection of their metal constituents (Co, Pt, and Re) by inductively coupled plasma mass spectrometry (ICP-MS). Vitamin B12 (cyano-cob(iii)alamin) and aquo-cob(iii)alamin [Cbl-OH2](+), which differ in the β-axial ligands (CN(-) and H2O, respectively), were included as control samples. The results indicated that B12 derivatives delivered cisplatin to both cellular cytosol and nuclei with an efficiency of one third compared to the uptake of free cisplatin cis-[Pt(II)Cl2(NH3)2]. In addition, uptake of charged B12 derivatives including [Cbl-OH2](+), [{Co}-CN-{cis-PtCl(NH3)2}](+), [{Re}-{Co}-CN-{cis-PtCl(NH3)2}](+), and [{Co}-CN-{trans-Pt(Cyt)(NH3)2}](2+) (Cyt = cytarabin) was high compared to neutral B12, which implied the existence of an additional internalization pathway for charged B12 vitamin analogs. The affinities of the charged B12 derivatives to the B12 transporters HC, IF and TC were similar to that of native vitamin B12. PMID:26739575

  17. Cellular automata modelling of SEIRS

    Institute of Scientific and Technical Information of China (English)

    Liu Quan-Xing; Jin Zhen

    2005-01-01

    In this paper the SEIRS epidemic spread is analysed, and a two-dimensional probability cellular automata model for SEIRS is presented. Each cellular automation cell represents a part of the population that may be found in one of five states of individuals: susceptible, exposed (or latency), infected, immunized (or recovered) and death. Here studied are the effects of two cases on the epidemic spread. i.e. the effects of non-segregation and segregation on the latency and the infected of population. The conclusion is reached that the epidemic will persist in the case of non-segregation but it will decrease in the case of segregation. The proposed model can serve as a basis for the development of algorithms to simulate real epidemics based on real data. Last we find the density series of the exposed and the infected will fluctuate near a positive equilibrium point, when the constant for the immunized is less than its corresponding constant τ0. Our theoretical results are verified by numerical simulations.

  18. Cellular functions of the microprocessor.

    Science.gov (United States)

    Macias, Sara; Cordiner, Ross A; Cáceres, Javier F

    2013-08-01

    The microprocessor is a complex comprising the RNase III enzyme Drosha and the double-stranded RNA-binding protein DGCR8 (DiGeorge syndrome critical region 8 gene) that catalyses the nuclear step of miRNA (microRNA) biogenesis. DGCR8 recognizes the RNA substrate, whereas Drosha functions as an endonuclease. Recent global analyses of microprocessor and Dicer proteins have suggested novel functions for these components independent of their role in miRNA biogenesis. A HITS-CLIP (high-throughput sequencing of RNA isolated by cross-linking immunoprecipitation) experiment designed to identify novel substrates of the microprocessor revealed that this complex binds and regulates a large variety of cellular RNAs. The microprocessor-mediated cleavage of several classes of RNAs not only regulates transcript levels, but also modulates alternative splicing events, independently of miRNA function. Importantly, DGCR8 can also associate with other nucleases, suggesting the existence of alternative DGCR8 complexes that may regulate the fate of a subset of cellular RNAs. The aim of the present review is to provide an overview of the diverse functional roles of the microprocessor.

  19. Universal map for cellular automata

    Energy Technology Data Exchange (ETDEWEB)

    García-Morales, V., E-mail: vmorales@ph.tum.de [Institute for Advanced Study – Technische Universität München, Lichtenbergstr. 2a, D-85748 Garching (Germany)

    2012-08-20

    A universal map is derived for all deterministic 1D cellular automata (CAs) containing no freely adjustable parameters and valid for any alphabet size and any neighborhood range (including non-symmetrical neighborhoods). The map can be extended to an arbitrary number of dimensions and topologies and to arbitrary order in time. Specific CA maps for the famous Conway's Game of Life and Wolfram's 256 elementary CAs are given. An induction method for CAs, based in the universal map, allows mathematical expressions for the orbits of a wide variety of elementary CAs to be systematically derived. -- Highlights: ► A universal map is derived for all deterministic 1D cellular automata (CA). ► The map is generalized to 2D for Von Neumann, Moore and hexagonal neighborhoods. ► A map for all Wolfram's 256 elementary CAs is derived. ► A map for Conway's “Game of Life” is obtained.

  20. Melanoma screening with cellular phones.

    Directory of Open Access Journals (Sweden)

    Cesare Massone

    Full Text Available BACKGROUND: Mobile teledermatology has recently been shown to be suitable for teledermatology despite limitations in image definition in preliminary studies. The unique aspect of mobile teledermatology is that this system represents a filtering or triage system, allowing a sensitive approach for the management of patients with emergent skin diseases. METHODOLOGY/PRINCIPAL FINDINGS: In this study we investigated the feasibility of teleconsultation using a new generation of cellular phones in pigmented skin lesions. 18 patients were selected consecutively in the Pigmented Skin Lesions Clinic of the Department of Dermatology, Medical University of Graz, Graz (Austria. Clinical and dermoscopic images were acquired using a Sony Ericsson with a built-in two-megapixel camera. Two teleconsultants reviewed the images on a specific web application (http://www.dermahandy.net/default.asp where images had been uploaded in JPEG format. Compared to the face-to-face diagnoses, the two teleconsultants obtained a score of correct telediagnoses of 89% and of 91.5% reporting the clinical and dermoscopic images, respectively. CONCLUSIONS/SIGNIFICANCE: The present work is the first study performing mobile teledermoscopy using cellular phones. Mobile teledermatology has the potential to become an easy applicable tool for everyone and a new approach for enhanced self-monitoring for skin cancer screening in the spirit of the eHealth program of the European Commission Information for Society and Media.

  1. Two Phase Flow Simulation Using Cellular Automata

    International Nuclear Information System (INIS)

    channel. It was noticed that the CHF phenomena generally starts in cells located at the boundary.The fact that the phenomena can be well described by using simple rules shows that the related physics and even the physics with stochastic characteristics, has been captured with t his simple model. Some fractal properties of automata were also studied, arriving at the conclusion that the internal dynamics present fractal characteristics.Findings hint towards a new approach to solve open issues.Finally, a calculus related to the Australian reactor designed by INVAP was performed.The problem consists of a simulation of helium bubble production by the mechanism of mass diffusion in heavy water.It was verified that cellular automata systems are a powerful tool for describing problems with high complexity and short reach interactions between components.It was proved that two-phase flows could be described very well with this model.The approach is a powerful tool to describe non-equilibrium features, such as boiling flow development and interfacial topological transitions.A novel computer model of boiling crisis was encountered following this type of analysis.By means of this research, without invalidating the important advances obtained in other directions, a new tool is offered which can be useful regarding the modeling of boiling heat transfer systems

  2. Discrete geodesics and cellular automata

    CERN Document Server

    Arrighi, Pablo

    2015-01-01

    This paper proposes a dynamical notion of discrete geodesics, understood as straightest trajectories in discretized curved spacetime. The notion is generic, as it is formulated in terms of a general deviation function, but readily specializes to metric spaces such as discretized pseudo-riemannian manifolds. It is effective: an algorithm for computing these geodesics naturally follows, which allows numerical validation---as shown by computing the perihelion shift of a Mercury-like planet. It is consistent, in the continuum limit, with the standard notion of timelike geodesics in a pseudo-riemannian manifold. Whether the algorithm fits within the framework of cellular automata is discussed at length. KEYWORDS: Discrete connection, parallel transport, general relativity, Regge calculus.

  3. Cellular compartmentalization of secondary metabolism

    Directory of Open Access Journals (Sweden)

    H. Corby eKistler

    2015-02-01

    Full Text Available Fungal secondary metabolism is often considered apart from the essential housekeeping functions of the cell. However, there are clear links between fundamental cellular metabolism and the biochemical pathways leading to secondary metabolite synthesis. Besides utilizing key biochemical precursors shared with the most essential processes of the cell (e.g. amino acids, acetyl CoA, NADPH, enzymes for secondary metabolite synthesis are compartmentalized at conserved subcellular sites that position pathway enzymes to use these common biochemical precursors. Co-compartmentalization of secondary metabolism pathway enzymes also may function to channel precursors, promote pathway efficiency and sequester pathway intermediates and products from the rest of the cell. In this review we discuss the compartmentalization of three well-studied fungal secondary metabolite biosynthetic pathways for penicillin G, aflatoxin and deoxynivalenol, and summarize evidence used to infer subcellular localization. We also discuss how these metabolites potentially are trafficked within the cell and may be exported.

  4. Monkey hybrid stem cells develop cellular features of Huntington's disease

    Directory of Open Access Journals (Sweden)

    Lorthongpanich Chanchao

    2010-02-01

    Full Text Available Abstract Background Pluripotent stem cells that are capable of differentiating into different cell types and develop robust hallmark cellular features are useful tools for clarifying the impact of developmental events on neurodegenerative diseases such as Huntington's disease. Additionally, a Huntington's cell model that develops robust pathological features of Huntington's disease would be valuable for drug discovery research. Results To test this hypothesis, a pluripotent Huntington's disease monkey hybrid cell line (TrES1 was established from a tetraploid Huntington's disease monkey blastocyst generated by the fusion of transgenic Huntington's monkey skin fibroblast and a wild-type non-transgenic monkey oocyte. The TrES1 developed key Huntington's disease cellular pathological features that paralleled neural development. It expressed mutant huntingtin and stem cell markers, was capable of differentiating to neural cells, and developed teratoma in severely compromised immune deficient (SCID mice. Interestingly, the expression of mutant htt, the accumulation of oligomeric mutant htt and the formation of intranuclear inclusions paralleled neural development in vitro , and even mutant htt was ubiquitously expressed. This suggests the development of Huntington's disease cellular features is influenced by neural developmental events. Conclusions Huntington's disease cellular features is influenced by neural developmental events. These results are the first to demonstrate that a pluripotent stem cell line is able to mimic Huntington's disease progression that parallels neural development, which could be a useful cell model for investigating the developmental impact on Huntington's disease pathogenesis.

  5. Thermal effects of radiation from cellular telephones

    Science.gov (United States)

    Wainwright, Peter

    2000-08-01

    A finite element thermal model of the head has been developed to calculate temperature rises generated in the brain by radiation from cellular telephones and similar electromagnetic devices. A 1 mm resolution MRI dataset was segmented semiautomatically, assigning each volume element to one of ten tissue types. A finite element mesh was then generated using a fully automatic tetrahedral mesh generator developed at NRPB. There are two sources of heat in the model: firstly the natural metabolic heat production; and secondly the power absorbed from the electromagnetic field. The SAR was derived from a finite difference time domain model of the head, coupled to a model `mobile phone', namely a quarter-wavelength antenna mounted on a metal box. The steady-state temperature distribution was calculated using the standard Pennes `bioheat equation'. In the normal cerebral cortex the high blood perfusion rate serves to provide an efficient cooling mechanism. In the case of equipment generally available to the public, the maximum temperature rise found in the brain was about 0.1 °C. These results will help in the further development of criteria for exposure guidelines, and the technique developed may be used to assess temperature rises associated with SARs for different types of RF exposure.

  6. Whole-Organism Cellular Pathology: A Systems Approach to Phenomics.

    Science.gov (United States)

    Cheng, K C; Katz, S R; Lin, A Y; Xin, X; Ding, Y

    2016-01-01

    Phenotype is defined as the state of an organism resulting from interactions between genes, environment, disease, molecular mechanisms, and chance. The purpose of the emerging field of phenomics is to systematically determine and measure phenotypes across biology for the sake of understanding. Phenotypes can affect more than one cell type and life stage, so ideal phenotyping would include the state of every cell type within the context of both tissue architecture and the whole organism at each life stage. In medicine, high-resolution anatomic assessment of phenotype is obtained from histology. Histology's interpretative power, codified by Virchow as cellular pathology, is derived from its ability to discern diagnostic and characteristic cellular changes in diseased tissues. Cellular pathology is observed in every major human disease and relies on the ability of histology to detect cellular change in any cell type due to unbiased pan-cellular staining, even in optically opaque tissues. Our laboratory has shown that histology is far more sensitive than stereomicroscopy for detecting phenotypes in zebrafish mutants. Those studies have also shown that more complete sampling, greater consistency in sample orientation, and the inclusion of phenotypes extending over longer length scales would provide greater coverage of common phenotypes. We are developing technical approaches to achieve an ideal detection of cellular pathology using an improved form of X-ray microtomography that retains the strengths and addresses the weaknesses of histology as a screening tool. We are using zebrafish as a vertebrate model based on the overlaps between zebrafish and mammalian tissue architecture, and a body size small enough to allow whole-organism, volumetric imaging at cellular resolution. Automation of whole-organism phenotyping would greatly increase the value of phenomics. Potential societal benefits would include reduction in the cost of drug development, a reduction in the

  7. Integración de los actores e integralidad de las acciones en las políticas de desarrollo para la región del Golfo de Nicoya.

    Directory of Open Access Journals (Sweden)

    Oscar Pacheco Urpí

    2016-03-01

    Full Text Available En el Golfo de Nicoya existe una diversidad de factores que influyen, tanto desde el punto de vista cultural y social y de las actividades productivas y urbanas, así como de las potestades de las instituciones del Estado y Universitarias. Indicadores como: niveles crecientes de la contaminación, la disminución de los recursos pesqueros, el aumento desmedido de la población pesquera y el deterioro paulatino en la calidad del ambiente y de vida de las comunidades costeras, muestran claramente el origen de estos fenómenos y por lo general, se tienen definidos los objetivos para alcanzar un Desarrollo Sostenible. Sin embargo, por muchos años, se ha venido percibiendo una constancia en el proceso de deterioro, sin que se logre amainar (salvo algunos casos muy concretos y mucho menos revertir el proceso. Esto no quiere decir, que no se haya tenido la preocupación y no se haya tratado de solucionar los problemas a través del desarrollo de políticas y acciones, pero no se han logrado establecer las estrategias apropiadas que permitan tomar el camino del Desarrollo. En este sentido, si bien, se tiene claridad de que es fundamental y se ha hecho los esfuerzos de ordenar, a través de la coordinación interinstitucional e intersectorial, la intervención de los diferentes actores, la verdad es que no se ha logrado la integración y se continúan dando las acciones individuales y dispersas, por lo que no se ha logrado encontrar la estructura idónea que permita constituir las acciones en un proceso común y participativo, reconocida y aceptada por los diferentes sectores, gubernamentales y no gubernamentales. Por otra parte, los enfoques y el desarrollo de acciones, probablemente, también influenciados por la falta de integración de los actores, han conllevado a que éstos no se contemplen de manera integral y no contengan la secuencia requerida de los factores para que los procesos, que inician con la investigación, culminen con la aceptaci

  8. Cellular phones: are they detrimental?

    Science.gov (United States)

    Salama, Osama E; Abou El Naga, Randa M

    2004-01-01

    The issue of possible health effects of cellular phones is very much alive in the public's mind where the rapid increase in the number of the users of cell phones in the last decade has increased the exposure of people to the electromagnetic fields (EMFs). Health consequences of long term use of mobile phones are not known in detail but available data indicates the development of non specific annoying symptoms on acute exposure to mobile phone radiations. In an attempt to determine the prevalence of such cell phones associated health manifestations and the factors affecting their occurrence, a cross sectional study was conducted in five randomly selected faculties of Alexandria University. Where, 300 individuals including teaching staff, students and literate employee were equally allocated and randomly selected among the five faculties. Data about mobile phone's users and their medical history, their pattern of mobile usage and the possible deleterious health manifestations associated with cellular phone use was collected. The results revealed 68% prevalence of mobile phone usage, nearly three quarters of them (72.5%) were complainers of the health manifestations. They suffered from headache (43%), earache (38.3%), sense of fatigue (31.6%), sleep disturbance (29.5%), concentration difficulty (28.5%) and face burning sensation (19.2%). Both univariate and multivariate analysis were consistent in their findings. Symptomatic users were found to have significantly higher frequency of calls/day, longer call duration and longer total duration of mobile phone usage/day than non symptomatic users. For headache both call duration and frequency of calls/day were the significant predicting factors for its occurrence (chi2 = 18.208, p = 0.0001). For earache, in addition to call duration, the longer period of owning the mobile phone were significant predictors (chi2 = 16.996, p = 0.0002). Sense of fatigue was significantly affected by both call duration and age of the user

  9. Radiation, nitric oxide and cellular death

    International Nuclear Information System (INIS)

    The mechanisms of radiation induced cellular death constitute an objective of research ever since the first biological effects of radiation were first observed. The explosion of information produced in the last 20 years calls for a careful analysis due to the apparent contradictory data related to the cellular system studied and the range of doses used. This review focuses on the role of the active oxygen species, in particular the nitric oxides, in its relevance as potential mediator of radiation induced cellular death

  10. Autophagy and mitophagy in cellular damage control

    Directory of Open Access Journals (Sweden)

    Jianhua Zhang

    2013-01-01

    Full Text Available Autophagy and mitophagy are important cellular processes that are responsible for breaking down cellular contents, preserving energy and safeguarding against accumulation of damaged and aggregated biomolecules. This graphic review gives a broad summary of autophagy and discusses examples where autophagy is important in controlling protein degradation. In addition we highlight how autophagy and mitophagy are involved in the cellular responses to reactive species and mitochondrial dysfunction. The key signaling pathways for mitophagy are described in the context of bioenergetic dysfunction.

  11. Modeling integrated cellular machinery using hybrid Petri-Boolean networks.

    Directory of Open Access Journals (Sweden)

    Natalie Berestovsky

    Full Text Available The behavior and phenotypic changes of cells are governed by a cellular circuitry that represents a set of biochemical reactions. Based on biological functions, this circuitry is divided into three types of networks, each encoding for a major biological process: signal transduction, transcription regulation, and metabolism. This division has generally enabled taming computational complexity dealing with the entire system, allowed for using modeling techniques that are specific to each of the components, and achieved separation of the different time scales at which reactions in each of the three networks occur. Nonetheless, with this division comes loss of information and power needed to elucidate certain cellular phenomena. Within the cell, these three types of networks work in tandem, and each produces signals and/or substances that are used by the others to process information and operate normally. Therefore, computational techniques for modeling integrated cellular machinery are needed. In this work, we propose an integrated hybrid model (IHM that combines Petri nets and Boolean networks to model integrated cellular networks. Coupled with a stochastic simulation mechanism, the model simulates the dynamics of the integrated network, and can be perturbed to generate testable hypotheses. Our model is qualitative and is mostly built upon knowledge from the literature and requires fine-tuning of very few parameters. We validated our model on two systems: the transcriptional regulation of glucose metabolism in human cells, and cellular osmoregulation in S. cerevisiae. The model produced results that are in very good agreement with experimental data, and produces valid hypotheses. The abstract nature of our model and the ease of its construction makes it a very good candidate for modeling integrated networks from qualitative data. The results it produces can guide the practitioner to zoom into components and interconnections and investigate them

  12. Optimized Cellular Core for Rotorcraft Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Patz Materials and Technologies proposes to develop a unique structural cellular core material to improve mechanical performance, reduce platform weight and lower...

  13. Efficiency of cellular information processing

    CERN Document Server

    Barato, Andre C; Seifert, Udo

    2014-01-01

    We show that a rate of conditional Shannon entropy reduction, characterizing the learning of an internal process about an external process, is bounded by the thermodynamic entropy production. This approach allows for the definition of an informational efficiency that can be used to study cellular information processing. We analyze three models of increasing complexity inspired by the E. coli sensory network, where the external process is an external ligand concentration jumping between two values. We start with a simple model for which ATP must be consumed so that a protein inside the cell can learn about the external concentration. With a second model for a single receptor we show that the rate at which the receptor learns about the external environment can be nonzero even without any dissipation inside the cell since chemical work done by the external process compensates for this learning rate. The third model is more complete, also containing adaptation. For this model we show inter alia that a bacterium i...

  14. Molecular and cellular limits to somatosensory specificity

    Directory of Open Access Journals (Sweden)

    Viana Félix

    2008-04-01

    involved primarily in nerve impulse generation can also influence the gating of transducing channels, dramatically modifying their activation profile. Thus, we propose that the capacity exhibited by the different functional types of somatosensory receptor neurons to preferentially detect and encode specific stimuli into a discharge of nerve impulses, appears to result of a characteristic combinatorial expression of different ion channels in each neuronal type that finally determines their transduction and impulse firing properties. Transduction channels don't operate in isolation and their cellular context should also be taken into consideration to fully understand their function. Moreover, the inhomogeneous distribution of transduction and voltage-gated channels at soma, axonal branches and peripheral endings of primary sensory neurons influences the characteristics of the propagated impulse discharge that encodes the properties of the stimulus. Alteration of this concerted operation of ion channels in pathological conditions may underlie the changes in excitability accompanying peripheral sensory neuron injuries.

  15. Geometric domains in cellular radiobiology

    International Nuclear Information System (INIS)

    A joint consideration of experimental findings and microdosimetric facts leads to the conclusion that the biological effects of ionizing radiations are determined by local concentration of absorbed energy at two types of sites that differ greatly in magnitude. They are of the scale of nanometres in primary DNA damage, and of micrometres in the especially deleterious combinations of damaged DNA fragments. Both kinds depend on LET; however, at the nanometre level the dependence is much smaller. Depending on the relative contribution of the two types, the RBE values for observed biological effects can vary considerably but they may be assumed to be larger for cell transformation than for cell killing. (author)

  16. Interrogating cellular fate decisions with high-throughput arrays of multiplexed cellular communities.

    Science.gov (United States)

    Chen, Sisi; Bremer, Andrew W; Scheideler, Olivia J; Na, Yun Suk; Todhunter, Michael E; Hsiao, Sonny; Bomdica, Prithvi R; Maharbiz, Michel M; Gartner, Zev J; Schaffer, David V

    2016-01-01

    Recreating heterotypic cell-cell interactions in vitro is key to dissecting the role of cellular communication during a variety of biological processes. This is especially relevant for stem cell niches, where neighbouring cells provide instructive inputs that govern cell fate decisions. To investigate the logic and dynamics of cell-cell signalling networks, we prepared heterotypic cell-cell interaction arrays using DNA-programmed adhesion. Our platform specifies the number and initial position of up to four distinct cell types within each array and offers tunable control over cell-contact time during long-term culture. Here, we use the platform to study the dynamics of single adult neural stem cell fate decisions in response to competing juxtacrine signals. Our results suggest a potential signalling hierarchy between Delta-like 1 and ephrin-B2 ligands, as neural stem cells adopt the Delta-like 1 phenotype of stem cell maintenance on simultaneous presentation of both signals. PMID:26754526

  17. Cellular and Matrix Contributions to Tissue Construct Stiffness Increase with Cellular Concentration

    Science.gov (United States)

    Marquez, J. Pablo; Genin, Guy M.; Pryse, Kenneth M.; Elson, Elliot L.

    2013-01-01

    The mechanics of bio-artificial tissue constructs result from active and passive contributions of cells and extracellular matrix (ECM). We delineated these for a fibroblast-populated matrix (FPM) consisting of chick embryo fibroblast cells in a type I collagen ECM through mechanical testing, mechanical modeling, and selective biochemical elimination of tissue components. From a series of relaxation tests, we found that contributions to overall tissue mechanics from both cells and ECM increase exponentially with the cell concentration. The force responses in these relaxation tests exhibited a logarithmic decay over the 3600 second test duration. The amplitudes of these responses were nearly linear with the amplitude of the applied stretch. The active component of cellular forces rose dramatically for FPMs containing higher cell concentrations. PMID:16874557

  18. Vitamin E Supplementation Delays Cellular Senescence In Vitro.

    Science.gov (United States)

    La Fata, Giorgio; Seifert, Nicole; Weber, Peter; Mohajeri, M Hasan

    2015-01-01

    Vitamin E is an important antioxidant that protects cells from oxidative stress-induced damage, which is an important contributor to the progression of ageing. Ageing can be studied in vitro using primary cells reaching a state of irreversible growth arrest called senescence after a limited number of cellular divisions. Generally, the most utilized biomarker of senescence is represented by the expression of the senescence associated β-galactosidase (SA-β-gal). We aimed here to study the possible effects of vitamin E supplementation in two different human primary cell types (HUVECs and fibroblasts) during the progression of cellular senescence. Utilizing an unbiased automated system, based on the detection of the SA-β-gal, we quantified cellular senescence in vitro and showed that vitamin E supplementation reduced the numbers of senescent cells during progression of ageing. Acute vitamin E supplementation did not affect cellular proliferation, whereas it was decreased after chronic treatment. Mechanistically, we show that vitamin E supplementation acts through downregulation of the expression of the cycline dependent kinase inhibitor P21. The data obtained from this study support the antiageing properties of vitamin E and identify possible mechanisms of action that warrant further investigation. PMID:26613084

  19. Vitamin E Supplementation Delays Cellular Senescence In Vitro

    Directory of Open Access Journals (Sweden)

    Giorgio La Fata

    2015-01-01

    Full Text Available Vitamin E is an important antioxidant that protects cells from oxidative stress-induced damage, which is an important contributor to the progression of ageing. Ageing can be studied in vitro using primary cells reaching a state of irreversible growth arrest called senescence after a limited number of cellular divisions. Generally, the most utilized biomarker of senescence is represented by the expression of the senescence associated β-galactosidase (SA-β-gal. We aimed here to study the possible effects of vitamin E supplementation in two different human primary cell types (HUVECs and fibroblasts during the progression of cellular senescence. Utilizing an unbiased automated system, based on the detection of the SA-β-gal, we quantified cellular senescence in vitro and showed that vitamin E supplementation reduced the numbers of senescent cells during progression of ageing. Acute vitamin E supplementation did not affect cellular proliferation, whereas it was decreased after chronic treatment. Mechanistically, we show that vitamin E supplementation acts through downregulation of the expression of the cycline dependent kinase inhibitor P21. The data obtained from this study support the antiageing properties of vitamin E and identify possible mechanisms of action that warrant further investigation.

  20. Multiple cellular origins and molecular evolution of intrahepatic cholangiocarcinoma.

    Science.gov (United States)

    Wei, Miaoyan; Lü, Lisheng; Lin, Peiyi; Chen, Zhisheng; Quan, Zhiwei; Tang, Zhaohui

    2016-09-01

    Intrahepatic cholangiocarcinoma (ICC) is an aggressive malignancy associated with unfavorable prognosis and for which no effective treatments are available. Its molecular pathogenesis is poorly understood. Genome-wide sequencing and high-throughput technologies have provided critical insights into the molecular basis of ICC while sparking a heated debate on the cellular origin. Cancer exhibits variabilities in origin, progression and cell biology. Recent evidence suggests that ICC has multiple cellular origins, including differentiated hepatocytes; intrahepatic biliary epithelial cells (IBECs)/cholangiocytes; pluripotent stem cells, such as hepatic stem/progenitor cells (HPCs) and biliary tree stem/progenitor cells (BTSCs); and peribiliary gland (PBG). However, both somatic mutagenesis and epigenomic features are highly cell type-specific. Multiple cellular origins may have profoundly different genomic landscapes and key signaling pathways, driving phenotypic variation and thereby posing significant challenges to personalized medicine in terms of achieving the optimal drug response and patient outcome. Considering this information, we have summarized the latest experimental evidence and relevant literature to provide an up-to-date view of the cellular origin of ICC, which will contribute to establishment of a hierarchical model of carcinogenesis and allow for improvement of the anatomical-based classification of ICC. These new insights have important implications for both the diagnosis and treatment of ICC patients. PMID:26940139

  1. Molecular and cellular mechanisms of adipogenesis

    Directory of Open Access Journals (Sweden)

    Aleksander Dmitrievich Egorov

    2015-03-01

    Full Text Available The main components of metabolic syndrome include insulin resistance, hypertriglyceridemia and arterial hypertension. Obesity is the cause of metabolic syndrome, mainly as a consequence of the endocrine function of adipose tissue. The volume of adipose tissue depends on the size of individual adipocytes and on their number. The number of adipocytes increases as a result of enhanced adipocyte differentiation. The transcriptional cascade that regulates this differentiation has been well studied. The major adipogenic transcription factor peroxisome proliferator-activated receptor gamma is a ligand-activated nuclear receptor with essential roles in adipogenesis. Its ligands are used to treat metabolic syndrome and type 2 diabetes mellitus. The present article describes the basic molecular and cellular mechanisms of adipogenesis and discusses the impact of insulin, glucocorticoids, cyclic adenosine monophosphate-activating agents, nuclear receptors and transcription factors on the process of adipogenesis. New regulatory regions of the genome that are capable of binding multiple transcription factors are described, and the most promising drug targets for the treatment of metabolic syndrome and obesity, including the homeodomain proteins Pbx1 and Prep1, are discussed.

  2. Analytical Modeling of Uplink Cellular Networks

    CERN Document Server

    Novlan, Thomas D; Andrews, Jeffrey G

    2012-01-01

    Cellular uplink analysis has typically been undertaken by either a simple approach that lumps all interference into a single deterministic or random parameter in a Wyner-type model, or via complex system level simulations that often do not provide insight into why various trends are observed. This paper proposes a novel middle way that is both accurate and also results in easy-to-evaluate integral expressions based on the Laplace transform of the interference. We assume mobiles and base stations are randomly placed in the network with each mobile pairing up to its closest base station. The model requires two important changes compared to related recent work on the downlink. First, dependence is introduced between the user and base station point processes to make sure each base station serves a single mobile in the given resource block. Second, per-mobile power control is included, which further couples the locations of the mobiles and their receiving base stations. Nevertheless, we succeed in deriving the cov...

  3. Pulsed feedback defers cellular differentiation.

    Directory of Open Access Journals (Sweden)

    Joe H Levine

    2012-01-01

    Full Text Available Environmental signals induce diverse cellular differentiation programs. In certain systems, cells defer differentiation for extended time periods after the signal appears, proliferating through multiple rounds of cell division before committing to a new fate. How can cells set a deferral time much longer than the cell cycle? Here we study Bacillus subtilis cells that respond to sudden nutrient limitation with multiple rounds of growth and division before differentiating into spores. A well-characterized genetic circuit controls the concentration and phosphorylation of the master regulator Spo0A, which rises to a critical concentration to initiate sporulation. However, it remains unclear how this circuit enables cells to defer sporulation for multiple cell cycles. Using quantitative time-lapse fluorescence microscopy of Spo0A dynamics in individual cells, we observed pulses of Spo0A phosphorylation at a characteristic cell cycle phase. Pulse amplitudes grew systematically and cell-autonomously over multiple cell cycles leading up to sporulation. This pulse growth required a key positive feedback loop involving the sporulation kinases, without which the deferral of sporulation became ultrasensitive to kinase expression. Thus, deferral is controlled by a pulsed positive feedback loop in which kinase expression is activated by pulses of Spo0A phosphorylation. This pulsed positive feedback architecture provides a more robust mechanism for setting deferral times than constitutive kinase expression. Finally, using mathematical modeling, we show how pulsing and time delays together enable "polyphasic" positive feedback, in which different parts of a feedback loop are active at different times. Polyphasic feedback can enable more accurate tuning of long deferral times. Together, these results suggest that Bacillus subtilis uses a pulsed positive feedback loop to implement a "timer" that operates over timescales much longer than a cell cycle.

  4. Simulation of plant communities with a cellular automaton

    Energy Technology Data Exchange (ETDEWEB)

    Gassmann, F. [Paul Scherrer Inst. (PSI), Villigen (Switzerland)

    1999-08-01

    With a modelling approach based on cellular automata, five observed types of plant development can be simulated. In addition, the proposed model shows a strong tendency towards the formation of patches and a high degree of dynamical and structural instability leading to limits of predictability for the asymptotic solution chosen by the system among several possible metastable patterns (multistability). Further, external fluctuations can be shown to have advantages for certain plant types. The presented model unifies the fundamental dichotomy in vegetation dynamics between determinism (understood as predictability) and disorder (chance effects) by showing the outcome of both classical theories as special cases. (author) 2 figs., 4 refs.

  5. La responsabilidad social de las organizaciones: ¿Factor de ventaja competitiva? Las acciones sociales de las organizaciones en relación con el tejido social La responsabilidad social de las organizaciones: ¿Factor de ventaja competitiva? Las acciones sociales de las organizaciones en relación con el tejido social

    Directory of Open Access Journals (Sweden)

    Mery Gallego Franco

    2006-06-01

    Full Text Available Mirar las organizaciones desde un enfoque sistémico, permite verlas como un conjunto de partesinteractuantes e interdependientes que en su dinámica conforman un sistema mayor que es lasociedad. Desde esta perspectiva puede comprenderse que cada organización afecta y es afectadapor el entorno en el cual se desenvuelve y que esta mutua relación —de beneficio o perjuicio—determina, en un alto grado, su supervivencia. Ésta es una forma de entender lo que hoy seconoce como Responsabilidad social; sin embargo, este concepto tiene antecedentes desde finalesdel siglo XIX y diversas manifestaciones a lo largo del siglo XX. Más recientemente los efectosnegativos de la globalización, entre otras razones, han forzado el surgimiento de normas, códigosde conducta, guías y otras acciones orientadas a contrarrestar dichos efectos buscando crearcondiciones de vida laboral y social más dignas.El cumplimiento de la responsabilidad por parte de cada organización es un imperativo ético,moral y social pues es desde el cumplimiento de ésta como se contribuye al fortalecimiento y/o recuperación del Tejido Social y a la construcción de lo que se conoce como Capital Social. Toda organización debe cumplir responsabilidades sociales tanto en su interior, conel personal que en ella labora, como con los distintos sectores con los cuales interactúaconocidos hoy como Stakeholders.Approaching organizations from a systemic point of view allows us to see them as a group of interactive and interdependent parts of a larger system called society. This perspective lets us understand that each organization simultaneously affects and is affected by its environment, and this reciprocal relationship —of benefit or damage— largely determines its survival. This paper intends to explain, in a certain way, what is known today as social responsibility, although this concept has antecedents that go back to the end of the Nineteenth century and diverse

  6. Gerenciamento do cuidado: ações dos enfermeiros em um serviço hospitalar de emergência Gerenciamiento del cuidado: acciones de los enfermeros en un servicio hospitalario de emergencia Care management: nurses' actions in a hospital emergency service

    Directory of Open Access Journals (Sweden)

    José Luís Guedes dos Santos

    2011-12-01

    Full Text Available Estudo com objetivo de analisar as ações de gerenciamento do cuidado realizadas pelos enfermeiros em um serviço hospitalar de emergência. Pesquisa qualitativa do tipo estudo de caso, realizada com enfermeiros do Serviço de Emergência de um Hospital Universitário da região sul do Brasil. Os dados foram coletados por meio de observação participante e entrevista semi-estruturada e analisados pela técnica de análise temática. Os resultados evidenciam ações dos enfermeiros no planejamento do cuidado, previsão e provisão de recursos, supervisão, liderança e capacitação da equipe de enfermagem. No planejamento do cuidado, identifica-se a execução do processo de enfermagem e o controle da realização dos exames laboratoriais e radiológicos. As ações de previsão e provisão de recursos foram: elaboração de escala mensal de funcionários, distribuição diária dos funcionários e gerenciamento de recursos materiais. A liderança favorece o planejamento da assistência, a coordenação da equipe de enfermagem e a delegação de atividades.Este estudio tuvo como objetivo analizar la actuación del enfermero en el gerenciamiento del cuidado en un servicio hospitalario de emergencia. Investigación cualitativa de tipo estudio de caso realizada con los enfermeros del Servicio de Emergencia de un Hospital Universitario de la Región Sur de Brasil. Los datos fueron recogidos a través de observación participante y entrevistas semi-estructuradas y analizados utilizando el análisis temático. Los resultados demostraron las acciones de los enfermeros en la planificación del cuidado, la previsión y provisión de recursos, supervisión, liderazgo y capacitación del equipo de enfermería. En la planificación del cuidado, se ha identificado la ejecución del proceso de enfermería y control de la realización de los exámenes de laboratorio y radiológicos. Las acciones de previsión y provisión de recursos fueron: escala mensual

  7. Cellular injury evidenced by impedance technology and infrared microspectroscopy

    Science.gov (United States)

    le Roux, K.; Prinsloo, L. C.; Meyer, D.

    2015-03-01

    Fourier Transform Infrared (FTIR) spectroscopy is finding increasing biological application, for example in the analysis of diseased tissues and cells, cell cycle studies and investigating the mechanisms of action of anticancer drugs. Cancer treatment studies routinely define the types of cell-drug responses as either total cell destruction by the drug (all cells die), moderate damage (cell deterioration where some cells survive) or reversible cell cycle arrest (cytostasis). In this study the loss of viability and related chemical stress experienced by cells treated with the medicinal plant, Plectranthus ciliatus, was investigated using real time cell electronic sensing (RT-CES) technology and FTIR microspectroscopy. The use of plants as medicines is well established and ethnobotany has proven that crude extracts can serve as treatments against various ailments. The aim of this study was to determine whether FTIR microspectroscopy would successfully distinguish between different types of cellular injury induced by a potentially anticancerous plant extract. Cervical adenocarcinoma (HeLa) cells were treated with a crude extract of Pciliatus and cells monitored using RT-CES to characterize the type of cellular responses induced. Cell populations were then investigated using FTIR microspectroscopy and statistically analysed using One-way Analysis of Variance (ANOVA) and Principal Component Analysis (PCA). The plant extract and a cancer drug control (actinomycin D) induced concentration dependent cellular responses ranging from nontoxic, cytostatic or cytotoxic. Thirteen spectral peaks (915 cm-1, 933 cm-1, 989 cm-1, 1192 cm-1, 1369 cm-1, 1437 cm-1, 1450 cm-1, 1546 cm-1, 1634 cm-1, 1679 cm-1 1772 cm-1, 2874 cm-1 and 2962 cm-1) associated with cytotoxicity were significantly (p value FTIR microspectroscopy confirmed that cytostatic cells were viable and could still recover while also describing early cellular stress related responses on a molecular level.

  8. From Cnn Dynamics to Cellular Wave Computers

    Science.gov (United States)

    Roska, Tamas

    2013-01-01

    Embedded in a historical overview, the development of the Cellular Wave Computing paradigm is presented, starting from the standard CNN dynamics. The theoretical aspects, the physical implementation, the innovation process, as well as the biological relevance are discussed in details. Finally, the latest developments, the physical versus virtual cellular machines, as well as some open questions are presented.

  9. Cellular encoding for interactive evolutionary robotics

    NARCIS (Netherlands)

    Gruau, F.C.; Quatramaran, K.

    1996-01-01

    This work reports experiments in interactive evolutionary robotics. The goal is to evolve an Artificial Neural Network (ANN) to control the locomotion of an 8-legged robot. The ANNs are encoded using a cellular developmental process called cellular encoding. In a previous work similar experiments ha

  10. Recent development of cellular manufacturing systems

    Indian Academy of Sciences (India)

    P K Arora; A Haleem; M K Singh

    2013-06-01

    Cellular manufacturing system has been proved a vital approach for batch and job shop production systems. Group technology has been an essential tool for developing a cellular manufacturing system. The paper aims to discuss various cell formation techniques and highlights the significant research work done in past over the years and attempts to points out the gap in research.

  11. The Universe as a Cellular System

    CERN Document Server

    Aragón-Calvo, Miguel A

    2014-01-01

    Cellular systems are observed everywhere in nature, from crystal domains in metals, soap froth and cucumber cells to the network of cosmological voids. Surprisingly, despite their disparate scale and origin all cellular systems follow certain scaling laws relating their geometry, topology and dynamics. Using a cosmological N-body simulation we found that the Cosmic Web, the largest known cellular system, follows the same scaling relations seen elsewhere in nature. Our results extend the validity of scaling relations in cellular systems by over 30 orders of magnitude in scale with respect to previous studies. The dynamics of cellular systems can be used to interpret local observations such as the local velocity anomaly as the result of a collapsing void in our cosmic backyard. Moreover, scaling relations depend on the curvature of space, providing an independent measure of geometry.

  12. [Plasticity of the cellular phenotype].

    Science.gov (United States)

    Chneiweiss, Hervé

    2011-01-01

    The tragical consequences of the Hiroshima and Nagasaki atomic bombs in 1945 were to lead to the discovery of hematopoietic stem cells and their phenotypic plasticity, in response to environmental factors. These concepts were much later extended to the founding cells of other tissues. In the following collection of articles, the mechanisms underlying this plasticity, at the frontiers of developmental biology and oncology, are illustrated in the case of various cell types of neural origin and of some tumours. PMID:21501574

  13. The thorny path linking cellular senescence to organismalaging

    Energy Technology Data Exchange (ETDEWEB)

    Patil, Christopher K.; Mian, Saira; Campisi, Judith

    2005-08-09

    Half a century is fast approaching since Hayflick and colleagues formally described the limited ability of normal human cells to proliferate in culture (Hayflick and Moorhead, 1961). This finding--that normal somatic cells, in contrast to cancer cells, cannot divide indefinitely--challenged the prevailing idea that cells from mortal multicellular organisms were intrinsically ''immortal'' (Carrell, 1912). It also spawned two hypotheses, essential elements of which persist today. The first held that the restricted proliferation of normal cells, now termed cellular senescence, suppresses cancer (Hayflick, 1965; Sager, 1991; Campisi, 2001). The second hypothesis, as explained in the article by Lorenzini et al., suggested that the limited proliferation of cells in culture recapitulated aspects of organismal aging (Hayflick, 1965; Martin, 1993). How well have these hypotheses weathered the ensuing decades? Before answering this question, we first consider current insights into the causes and consequences of cellular senescence. Like Lorenzini et al., we limit our discussion to mammals. We also focus on fibroblasts, the cell type studied by Lorenzini et al., but consider other types as well. We suggest that replicative capacity in culture is not a straightforward assessment, and that it correlates poorly with both longevity and body mass. We speculate this is due to the malleable and variable nature of replicative capacity, which renders it an indirect metric of qualitative and quantitative differences among cells to undergo senescence, a response that directly alters cellular phenotype and might indirectly alter tissue structure and function.

  14. Modeling and Analysis of Cellular Networks using Stochastic Geometry: A Tutorial

    KAUST Repository

    ElSawy, Hesham

    2016-03-22

    This paper presents a tutorial on stochastic geometry (SG) based analysis for cellular networks. This tutorial is distinguished by its depth with respect to wireless communication details and its focus on cellular networks. The paper starts by modeling and analyzing the baseband interference in a basic cellular network model. Then, it characterizes signal-tointerference- plus-noise-ratio (SINR) and its related performance metrics. In particular, a unified approach to conduct error probability, outage probability, and rate analysis is presented. Although the main focus of the paper is on cellular networks, the presented unified approach applies for other types of wireless networks that impose interference protection around receivers. The paper then extends the baseline unified approach to capture cellular network characteristics (e.g., frequency reuse, multiple antenna, power control, etc.). It also presents numerical examples associated with demonstrations and discussions. Finally, we point out future research directions.

  15. Identification of a novel Rev-interacting cellular protein

    Directory of Open Access Journals (Sweden)

    Werner Thomas

    2005-04-01

    Full Text Available Abstract Background Human cell types respond differently to infection by human immunodeficiency virus (HIV. Defining specific interactions between host cells and viral proteins is essential in understanding how viruses exploit cellular functions and the innate strategies underlying cellular control of HIV replication. The HIV Rev protein is a post-transcriptional inducer of HIV gene expression and an important target for interaction with cellular proteins. Identification of Rev-modulating cellular factors may eventually contribute to the design of novel antiviral therapies. Results Yeast-two hybrid screening of a T-cell cDNA library with Rev as bait led to isolation of a novel human cDNA product (16.4.1. 16.4.1-containing fusion proteins showed predominant cytoplasmic localization, which was dependent on CRM1-mediated export from the nucleus. Nuclear export activity of 16.4.1 was mapped to a 60 amino acid region and a novel transport signal identified. Interaction of 16.4.1 with Rev in human cells was shown in a mammalian two-hybrid assay and by colocalization of Rev and 16.4.1 in nucleoli, indicating that Rev can recruit 16.4.1 to the nucleus/nucleoli. Rev-dependent reporter expression was inhibited by overexpressing 16.4.1 and stimulated by siRNAs targeted to 16.4.1 sequences, demonstrating that 16.4.1 expression influences the transactivation function of Rev. Conclusion These results suggest that 16.4.1 may act as a modulator of Rev activity. The experimental strategies outlined in this study are applicable to the identification and biological characterization of further novel Rev-interacting cellular factors.

  16. Multi-cellular logistics of collective cell migration.

    Directory of Open Access Journals (Sweden)

    Masataka Yamao

    Full Text Available During development, the formation of biological networks (such as organs and neuronal networks is controlled by multicellular transportation phenomena based on cell migration. In multi-cellular systems, cellular locomotion is restricted by physical interactions with other cells in a crowded space, similar to passengers pushing others out of their way on a packed train. The motion of individual cells is intrinsically stochastic and may be viewed as a type of random walk. However, this walk takes place in a noisy environment because the cell interacts with its randomly moving neighbors. Despite this randomness and complexity, development is highly orchestrated and precisely regulated, following genetic (and even epigenetic blueprints. Although individual cell migration has long been studied, the manner in which stochasticity affects multi-cellular transportation within the precisely controlled process of development remains largely unknown. To explore the general principles underlying multicellular migration, we focus on the migration of neural crest cells, which migrate collectively and form streams. We introduce a mechanical model of multi-cellular migration. Simulations based on the model show that the migration mode depends on the relative strengths of the noise from migratory and non-migratory cells. Strong noise from migratory cells and weak noise from surrounding cells causes "collective migration," whereas strong noise from non-migratory cells causes "dispersive migration." Moreover, our theoretical analyses reveal that migratory cells attract each other over long distances, even without direct mechanical contacts. This effective interaction depends on the stochasticity of the migratory and non-migratory cells. On the basis of these findings, we propose that stochastic behavior at the single-cell level works effectively and precisely to achieve collective migration in multi-cellular systems.

  17. Markers of cellular senescence. Telomere shortening as a marker of cellular senescence.

    Science.gov (United States)

    Bernadotte, Alexandra; Mikhelson, Victor M; Spivak, Irina M

    2016-01-01

    The cellular senescence definition comes to the fact of cells irreversible proliferation disability. Besides the cell cycle arrest, senescent cells go through some morphological, biochemical, and functional changes which are the signs of cellular senescence. The senescent cells (including replicative senescence and stress-induced premature senescence) of all the tissues look alike. They are metabolically active and possess the set of characteristics in vitro and in vivo, which are known as biomarkers of aging and cellular senescence. Among biomarkers of cellular senescence telomere shortening is a rather elegant frequently used biomarker. Validity of telomere shortening as a marker for cellular senescence is based on theoretical and experimental data. PMID:26805432

  18. Energy management in wireless cellular and ad-hoc networks

    CERN Document Server

    Imran, Muhammad; Qaraqe, Khalid; Alouini, Mohamed-Slim; Vasilakos, Athanasios

    2016-01-01

    This book investigates energy management approaches for energy efficient or energy-centric system design and architecture and presents end-to-end energy management in the recent heterogeneous-type wireless network medium. It also considers energy management in wireless sensor and mesh networks by exploiting energy efficient transmission techniques and protocols. and explores energy management in emerging applications, services and engineering to be facilitated with 5G networks such as WBANs, VANETS and Cognitive networks. A special focus of the book is on the examination of the energy management practices in emerging wireless cellular and ad hoc networks. Considering the broad scope of energy management in wireless cellular and ad hoc networks, this book is organized into six sections covering range of Energy efficient systems and architectures; Energy efficient transmission and techniques; Energy efficient applications and services. .

  19. Methods for Determining the Cellular Functions of Vimentin Intermediate Filaments.

    Science.gov (United States)

    Ridge, Karen M; Shumaker, Dale; Robert, Amélie; Hookway, Caroline; Gelfand, Vladimir I; Janmey, Paul A; Lowery, Jason; Guo, Ming; Weitz, David A; Kuczmarski, Edward; Goldman, Robert D

    2016-01-01

    The type III intermediate filament protein vimentin was once thought to function mainly as a static structural protein in the cytoskeleton of cells of mesenchymal origin. Now, however, vimentin is known to form a dynamic, flexible network that plays an important role in a number of signaling pathways. Here, we describe various methods that have been developed to investigate the cellular functions of the vimentin protein and intermediate filament network, including chemical disruption, photoactivation and photoconversion, biolayer interferometry, soluble bead binding assay, three-dimensional substrate experiments, collagen gel contraction, optical-tweezer active microrheology, and force spectrum microscopy. Using these techniques, the contributions of vimentin to essential cellular processes can be probed in ever further detail.

  20. Macromolecular lesions and cellular radiation chemistry

    International Nuclear Information System (INIS)

    Our studies of the interaction of densely ionizing particles with macromolecules in the living cell may be divided into four parts: characterization of lesions to cellular DNA in the unmodified Bragg ionization curve; characterization of lesions to cellular DNA in the spread Bragg curve as used in radiation therapy; elucidation of the cellular radiation chemistry characteristic of high vs. low LET radiation qualities; and the introduction of novel techniques designed to give a better understanding of the fundamental properties of induction of lesions and their repair potentials in high LET radiation

  1. Cellular and molecular mechanisms in kidney fibrosis

    Science.gov (United States)

    Duffield, Jeremy S.

    2014-01-01

    Fibrosis is a characteristic feature of all forms of chronic kidney disease. Deposition of pathological matrix in the interstitial space and within the walls of glomerular capillaries as well as the cellular processes resulting in this deposition are increasingly recognized as important factors amplifying kidney injury and accelerating nephron demise. Recent insights into the cellular and molecular mechanisms of fibrogenesis herald the promise of new therapies to slow kidney disease progression. This review focuses on new findings that enhance understanding of cellular and molecular mechanisms of fibrosis, the characteristics of myofibroblasts, their progenitors, and molecular pathways regulating both fibrogenesis and its resolution. PMID:24892703

  2. Imaging in cellular and tissue engineering

    CERN Document Server

    Yu, Hanry

    2013-01-01

    Details on specific imaging modalities for different cellular and tissue engineering applications are scattered throughout articles and chapters in the literature. Gathering this information into a single reference, Imaging in Cellular and Tissue Engineering presents both the fundamentals and state of the art in imaging methods, approaches, and applications in regenerative medicine. The book underscores the broadening scope of imaging applications in cellular and tissue engineering. It covers a wide range of optical and biological applications, including the repair or replacement of whole tiss

  3. Cellular Signaling Pathways and Their Clinical Reflections

    Directory of Open Access Journals (Sweden)

    N. Ceren Sumer-Turanligil

    2010-06-01

    Full Text Available Cellular signaling pathways have important roles in cellular growth, differentiation, inflammatory response and apoptosis and in regulation of cellular responses under various chemical stimulators. Different proteins which belong to these pathways may be exposed to loss-of-function or gain-of-function mutations; this may lead to many clinical phenotypes including primarily cancer. In this review information about basic working principles of these pathways and diseases related to them are included. [Archives Medical Review Journal 2010; 19(3.000: 180-191

  4. Cellular Cell Bifurcation of Cylindrical Detonations

    Institute of Scientific and Technical Information of China (English)

    HAN Gui-Lai; JIANG Zong-Lin; WANG Chun; ZHANG Fan

    2008-01-01

    Cellular cell pattern evolution of cylindrically-diverging detonations is numerically simulated successfully by solving two-dimensional Euler equations implemented with an improved two-step chemical kinetic model. From the simulation, three cell bifurcation modes are observed during the evolution and referred to as concave front focusing, kinked and wrinkled wave front instability, and self-merging of cellular cells. Numerical research demonstrates that the wave front expansion resulted from detonation front diverging plays a major role in the cellular cell bifurcation, which can disturb the nonlinearly self-sustained mechanism of detonations and finally lead to cell bifurcations.

  5. Estudio para la implementación de acciones estratégicas en la Unidad Empresarial de Base Integral de Servicios Técnicos de la Empresa Agropecuaria Güira de Melena

    Directory of Open Access Journals (Sweden)

    Manuel Fernández Sánchez

    2011-01-01

    Full Text Available Las Unidades Empresariales de Base Integrales de Servicios Técnicos constituyen una nueva forma de organización dentro del sector agrícola. Estas unidades, aparte de brindar servicios de mecanización a las unidades de producción de su municipio, ofrecerán también los servicios de mantenimiento y reparación a la maquinaria agrícola, los equipos de riego y de transporte. Esta última tarea convierte a la actividad de servicio técnico en un fuerte eslabón dentro de la nueva organización, por lo que el establecimiento de antemano acciones de gestión de tipo estratégica será la clave del éxito de la misión de estas unidades. Tomando como referencia uno de los talleres seleccionados para convertirse en Unidad Empresarial de Base (UEB, ubicado en el municipio Güira de Melena al sur de La Habana, se realizó un estudio, el cual incluyó como primer aspecto la determinación del estado actual de los recursos de producción en el taller y de los trabajos de mantenimientos y reparaciones. A partir de este estudio se realizó un análisis prospectivo donde se identificaron la visión, la misión y los objetivos de la futura organización y se determinaron los facto-res de tipo internos y externos que inciden en estos momentos sobre la actividad de servicio técnico. Así mismo, se de-terminaron las principales fortalezas, debilidades, oportunidades y amenazas de la futura organización, todo lo cual sirvió de base para establecer acciones estratégicas y líneas de trabajo para el ulterior mejoramiento de los servicios prestados.

  6. Acciones finales discriminantes de voleibol en categorías de formación masculina: importancia del saque en los partidos igualados. (Discriminatory volleyball final actions in male formative stages: importance .

    Directory of Open Access Journals (Sweden)

    Carlos Dávila-Romero

    2012-04-01

    Full Text Available Resumen El objetivo principal del presente estudio fue constatar la importancia del saque en categorías de formación en voleibol masculina y determinar qué otras acciones son relevantes para la victoria en el quinto set de los partidos igualados. Se recogieron un total de 218 partidos (102 en categoría infantil y 116 en categoría cadete, 755 sets (354 en categoría infantil y 401 en categoría cadete y 744 jugadores (360 en categoría infantil y 384 en categoría cadete de los Campeonatos de España de Voleibol de Valladolid 2008 y Huelva 2009 (España en edad escolar masculino (12-16 años. Finalmente se analizaron sólo el quinto set de los partidos igualados. Los resultados del análisis discriminante, mostraron como el saque positivo en ambas categorías, el error en categoría infantil y el ataque positivo en categoría cadete predicen la victoria y/o derrota en el quinto set. Estos sugieren que en momentos decisivos de set y en ambas categorías, el acierto en las acciones ofensivas y el control del patrón técnico del saque es fundamental en estos partidos igualados.AbstractThe aim of this study was to confirm the importance of the serve on formatives stages male volleyball and determine what actions are relevant to the victory in the fifth set on equal matches. We collected a total of 218 matches (102 and 116 in infantile and cadet categories, 755 sets (354 and 401 in infantile and cadet categories and 744 players (360 and 384 in infantile and cadet categories of the Spain Volleyball Championships held in Huelva and Valladolid in 2008 and 2009 (Spain at school-age (12-16 years. Finally were analyzed only the fifth set of equal matches. The results of the discriminant analysis showed how the positive serve in both categories, the error in infantile category and in cadet positive attack may predict victory and / or defeat in the fifth set. They suggest that, in both categories, the success in offensive actions and control of the

  7. Reciprocal Control of the Circadian Clock and Cellular Redox State - a Critical Appraisal.

    Science.gov (United States)

    Putker, Marrit; O'Neill, John Stuart

    2016-01-01

    Redox signalling comprises the biology of molecular signal transduction mediated by reactive oxygen (or nitrogen) species. By specific and reversible oxidation of redox-sensitive cysteines, many biological processes sense and respond to signals from the intracellular redox environment. Redox signals are therefore important regulators of cellular homeostasis. Recently, it has become apparent that the cellular redox state oscillates in vivo and in vitro, with a period of about one day (circadian). Circadian time-keeping allows cells and organisms to adapt their biology to resonate with the 24-hour cycle of day/night. The importance of this innate biological time-keeping is illustrated by the association of clock disruption with the early onset of several diseases (e.g. type II diabetes, stroke and several forms of cancer). Circadian regulation of cellular redox balance suggests potentially two distinct roles for redox signalling in relation to the cellular clock: one where it is regulated by the clock, and one where it regulates the clock. Here, we introduce the concepts of redox signalling and cellular timekeeping, and then critically appraise the evidence for the reciprocal regulation between cellular redox state and the circadian clock. We conclude there is a substantial body of evidence supporting circadian regulation of cellular redox state, but that it would be premature to conclude that the converse is also true. We therefore propose some approaches that might yield more insight into redox control of cellular timekeeping.

  8. Cellular Subcompartments through Cytoplasmic Streaming.

    Science.gov (United States)

    Pieuchot, Laurent; Lai, Julian; Loh, Rachel Ann; Leong, Fong Yew; Chiam, Keng-Hwee; Stajich, Jason; Jedd, Gregory

    2015-08-24

    Cytoplasmic streaming occurs in diverse cell types, where it generally serves a transport function. Here, we examine streaming in multicellular fungal hyphae and identify an additional function wherein regimented streaming forms distinct cytoplasmic subcompartments. In the hypha, cytoplasm flows directionally from cell to cell through septal pores. Using live-cell imaging and computer simulations, we identify a flow pattern that produces vortices (eddies) on the upstream side of the septum. Nuclei can be immobilized in these microfluidic eddies, where they form multinucleate aggregates and accumulate foci of the HDA-2 histone deacetylase-associated factor, SPA-19. Pores experiencing flow degenerate in the absence of SPA-19, suggesting that eddy-trapped nuclei function to reinforce the septum. Together, our data show that eddies comprise a subcellular niche favoring nuclear differentiation and that subcompartments can be self-organized as a consequence of regimented cytoplasmic streaming.

  9. Precambrian sponges with cellular structures

    Science.gov (United States)

    Li; Chen; Hua

    1998-02-01

    Sponge remains have been identified in the Early Vendian Doushantuo phosphate deposit in central Guizhou (South China), which has an age of approximately 580 million years ago. Their skeletons consist of siliceous, monaxonal spicules. All are referred to as the Porifera, class Demospongiae. Preserved soft tissues include the epidermis, porocytes, amoebocytes, sclerocytes, and spongocoel. Among thousands of metazoan embryos is a parenchymella-type of sponge larvae having a shoe-shaped morphology and dense peripheral flagella. The presence of possible amphiblastula larva suggests that the calcareous sponges may have an extended history in the Late Precambrian. The fauna indicates that animals lived 40 to 50 million years before the Cambrian Explosion. PMID:9452391

  10. Global stability analysis on a class of cellular neural networks

    Institute of Scientific and Technical Information of China (English)

    ZHANG; Yi

    2001-01-01

    [1]Chua, L. O., Yang, L., Cellular neural networks: Theory, IEEE Trans. CAS, 1988, (10): 1257.[2]Chua, L. O., Yang, L., Cellular neural networks: Applications, IEEE Trans. CAS, 1988, (10): 1273.[3]Chua, L. O., Roska, T., The CNN paradigm, IEEE Trans. CAS-I, 1993, (3): 147.[4]Matsumoto, T. Chua, L. O., Suzuki, H., CNN cloning template: Connected component detector, IEEE Trans. CAS, 1990, (8): 633.[5]Cao, L, Sun, Y, Yu, J., A CNN-based signature verification system,Proc. ICONIP′95, Beijing, 1995, 913—916.[6]Roska, T., Chua, L. O., The CNN universal machine: An analogic array computer, IEEE Trans. CAS Ⅱ, 1993, (3): 163.[7]Chua, L. O., Roska, T., Stability of a class of nonreciprocal cellular neural networks, IEEE Trans. CAS, 1990, (3): 1520.[8]Roska, T., Wu, C. W., Balsi, M. Et al., Stability and dynamics of delay type general and cellular neural networks, IEEE Trans. CAS, 1992, (6): 487.[9]Roska, T., Wu, C. W., Chua, L. O., Stability of cellular neural networks with dominant nonlinear and delaytype templates, IEEE Trans. CAS, 1993, (4): 270.[10]Civalleri, P. P., On stability of cellular neural networks with delay, IEEE Trans. CAS-I, 1993, (3): 157.[11]Gilli, G., Stability of cellular neural network and delayed cellular neural networks with nonpositive templates and nonmonotonic output functions, IEEE Trans CAS-I, 1994, (8): 518.[12]Baldi, P., Atiya, A. F., How delays affect neural dynamics and learning, IEEE Trans. On Neural Networks, 1994, (4): 612.[13]Liao, X. X., Mathematic foundation of cellular neural networks (Ⅰ), Science in China, Ser. A, 1994, 37(9): 902.[14]Liao, X. X., Mathematic foundation of cellular neural networks (Ⅱ), Science in China, Ser. A, 1994, 37(9): 1037.[15]Zhang, Y., Global exponential stability and periodic solutions of delay Hopfild neural networks, International J. Sys. Sci., 1996, (2): 227.[16]Zhang Yi, Zhong, S. M., Li, Z. L., Periodic solutions and global

  11. Densities and entropies in cellular automata

    CERN Document Server

    Guillon, Pierre

    2012-01-01

    Following work by Hochman and Meyerovitch on multidimensional SFT, we give computability-theoretic characterizations of the real numbers that can appear as the topological entropies of one-dimensional and two-dimensional cellular automata.

  12. A Matrix Construction of Cellular Algebras

    Institute of Scientific and Technical Information of China (English)

    Dajing Xiang

    2005-01-01

    In this paper, we give a concrete method to construct cellular algebras from matrix algebras by specifying certain fixed matrices for the data of inflations. In particular,orthogonal matrices can be chosen for such data.

  13. The role of sirtuins in cellular homeostasis.

    Science.gov (United States)

    Kupis, Wioleta; Pałyga, Jan; Tomal, Ewa; Niewiadomska, Ewa

    2016-09-01

    Sirtuins are evolutionarily conserved nicotinamide adenine dinucleotide (NAD(+))-dependent lysine deacylases or ADP-ribosyltransferases. These cellular enzymes are metabolic sensors sensitive to NAD(+) levels that maintain physiological homeostasis in the animal and plant cells. PMID:27154583

  14. Optimized Cellular Core for Rotorcraft Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Patz Materials and Technologies has developed, produced and tested, as part of the Phase-I SBIR, a new form of composite cellular core material, named Interply...

  15. Cellular Defect May Be Linked to Parkinson's

    Science.gov (United States)

    ... 160862.html Cellular Defect May Be Linked to Parkinson's: Study Abnormality might apply to all forms of ... that may be common to all forms of Parkinson's disease. The defect plays a major role in ...

  16. MILLIMETER-WAVE EMISSIVITY OF CELLULAR SYSTEMS

    Science.gov (United States)

    A general analysis has been presented of the millimeter-wave and farinfrared spectroscopic properties of in vivo cellular systems, and of the boson radiative equilibrium with steady-state nonequilibrium molecular systems. The frequency threshhold of spectroscopic properties assoc...

  17. Cellular and molecular mechanisms in kidney fibrosis

    OpenAIRE

    Duffield, Jeremy S.

    2014-01-01

    Fibrosis is a characteristic feature of all forms of chronic kidney disease. Deposition of pathological matrix in the interstitial space and within the walls of glomerular capillaries as well as the cellular processes resulting in this deposition are increasingly recognized as important factors amplifying kidney injury and accelerating nephron demise. Recent insights into the cellular and molecular mechanisms of fibrogenesis herald the promise of new therapies to slow kidney disease progressi...

  18. Apoptotic regulation of epithelial cellular extrusion

    OpenAIRE

    De Andrade, Daniel,; Rosenblatt, Jody

    2011-01-01

    Cellular extrusion is a mechanism that removes dying cells from epithelial tissues to prevent compromising their barrier function. Extrusion occurs in all observed epithelia in vivo and can be modeled in vitro by inducing apoptosis in cultured epithelial monolayers. We established that actin and myosin form a ring that contracts in the surrounding cells that drives cellular extrusion. It is not clear, however, if all apoptotic pathways lead to extrusion and how apoptosis and extrusion are mol...

  19. Cellularity of certain quantum endomorphism algebras

    DEFF Research Database (Denmark)

    Andersen, Henning Haahr; Lehrer, Gus; Zhang, Ruibin

    2015-01-01

    For any ring A˜ such that Z[q±1∕2]⊆A˜⊆Q(q1∕2), let ΔA˜(d) be an A˜-form of the Weyl module of highest weight d∈N of the quantised enveloping algebra UA˜ of sl2. For suitable A˜, we exhibit for all positive integers r an explicit cellular structure for EndUA˜(ΔA˜(d)⊗r). This algebra and its cellular...

  20. Cellular Hyperproliferation and Cancer as Evolutionary Variables

    OpenAIRE

    Alvarado, Alejandro Sánchez

    2012-01-01

    Technological advances in biology have begun to dramatically change the way we think about evolution, development, health and disease. The ability to sequence the genomes of many individuals within a population, and across multiple species, has opened the door to the possibility of answering some long-standing and perplexing questions about our own genetic heritage. One such question revolves around the nature of cellular hyperproliferation. This cellular behavior is used to effect wound heal...

  1. Building mathematics cellular phone learning communities

    OpenAIRE

    Wajeeh M. Daher

    2011-01-01

    Researchers emphasize the importance of maintaining learning communities and environments. This article describes the building and nourishment of a learning community, one comprised of middle school students who learned mathematics out-of-class using the cellular phone. The building of the learning community was led by three third year pre-service teachers majoring in mathematics and computers. The pre-service teachers selected thirty 8th grade students to learn mathematics with the cellular ...

  2. Understanding cisplatin resistance using cellular models.

    OpenAIRE

    STORDAL, BRITTA KRISTINA

    2007-01-01

    PUBLISHED Many mechanisms of cisplatin resistance have been proposed from studies of cellular models of resistance including changes in cellular drug accumulation, detoxification of the drug, inhibition of apoptosis and repair of the DNA adducts. A series of resistant models were developed from CCRF-CEM leukaemia cells with increasing doses of cisplatin from 100 ng/ml. This produced increasing resistance up to 7-fold with a treatment dose of 1.6 ?g/ml. Cisplatin resistance i...

  3. Understanding cisplatin resistance using cellular models

    OpenAIRE

    Stordal, Britta; Davey, Mary

    2007-01-01

    Many mechanisms of cisplatin resistance have been proposed from studies of cellular models of resistance including changes in cellular drug accumulation, detoxification of the drug, inhibition of apoptosis and repair of the DNA adducts. A series of resistant models were developed from CCRF-CEM leukaemia cells with increasing doses of cisplatin from 100 ng/ml. This produced increasing resistance up to 7-fold with a treatment dose of 1.6 microg/ml. Cisplatin resistance in these cells correlated...

  4. On the Behavior Characteristics of Cellular Automata

    Institute of Scientific and Technical Information of China (English)

    CHEN Jin-cai; ZHANG Jiang-ling; FENG Dan

    2005-01-01

    In this paper, the inherent relationships between the running regulations and behavior characteristics of cellular automata are presented; an imprecise taxonomy of such systems is put forward; the three extreme cases of stable systems are discussed; and the illogicalness of evolutional strategies of cellular automata is analyzed. The result is suitable for the emulation and prediction of behavior of discrete dynamics systems; especially it can be taken as an important analysis means of dynamic performance of complex networks.

  5. Cellular Scaling Rules of Insectivore Brains

    OpenAIRE

    Sarko, Diana K.; Catania, Kenneth C.; Leitch, Duncan B.; Kaas, Jon H.; Herculano-Houzel, Suzana

    2009-01-01

    Insectivores represent extremes in mammalian body size and brain size, retaining various “primitive” morphological characteristics, and some species of Insectivora are thought to share similarities with small-bodied ancestral eutherians. This raises the possibility that insectivore brains differ from other taxa, including rodents and primates, in cellular scaling properties. Here we examine the cellular scaling rules for insectivore brains and demonstrate that insectivore scaling rules overla...

  6. Cellular scaling rules of insectivore brains

    OpenAIRE

    Sarko, Diana K.; Catania, Kenneth C.; Leitch, Duncan B.; Kaas, Jon H.; Suzana Herculano-Houzel

    2009-01-01

    Insectivores represent extremes in mammalian body size and brain size, retaining various “primitive” morphological characteristics, and some species of Insectivora are thought to share similarities with small-bodied ancestral eutherians. This raises the possibility that insectivore brains differ from other taxa, including rodents and primates, in cellular scaling properties. Here we examine the cellular scaling rules for insectivore brains and demonstrate that insectivore scaling ...

  7. Cellular scaling rules for primate brains

    OpenAIRE

    Herculano-Houzel, Suzana; Collins, Christine E.; Wong, Peiyan; Kaas, Jon H.

    2007-01-01

    Primates are usually found to have richer behavioral repertoires and better cognitive abilities than rodents of similar brain size. This finding raises the possibility that primate brains differ from rodent brains in their cellular composition. Here we examine the cellular scaling rules for primate brains and show that brain size increases approximately isometrically as a function of cell numbers, such that an 11× larger brain is built with 10× more neurons and ≈12× more nonneuronal cells of ...

  8. Polymersomes containing quantum dots for cellular imaging

    Directory of Open Access Journals (Sweden)

    Camblin M

    2014-05-01

    Full Text Available Marine Camblin,1 Pascal Detampel,1 Helene Kettiger,1 Dalin Wu,2 Vimalkumar Balasubramanian,1,* Jörg Huwyler1,*1Division of Pharmaceutical Technology, 2Department of Chemistry, University of Basel, Basel, Switzerland*These authors contributed equally to this workAbstract: Quantum dots (QDs are highly fluorescent and stable probes for cellular and molecular imaging. However, poor intracellular delivery, stability, and toxicity of QDs in biological compartments hamper their use in cellular imaging. To overcome these limitations, we developed a simple and effective method to load QDs into polymersomes (Ps made of poly(dimethylsiloxane-poly(2-methyloxazoline (PDMS-PMOXA diblock copolymers without compromising the characteristics of the QDs. These Ps showed no cellular toxicity and QDs were successfully incorporated into the aqueous compartment of the Ps as confirmed by transmission electron microscopy, fluorescence spectroscopy, and fluorescence correlation spectroscopy. Ps containing QDs showed colloidal stability over a period of 6 weeks if stored in phosphate-buffered saline (PBS at physiological pH (7.4. Efficient intracellular delivery of Ps containing QDs was achieved in human liver carcinoma cells (HepG2 and was visualized by confocal laser scanning microscopy (CLSM. Ps containing QDs showed a time- and concentration-dependent uptake in HepG2 cells and exhibited better intracellular stability than liposomes. Our results suggest that Ps containing QDs can be used as nanoprobes for cellular imaging.Keywords: quantum dots, polymersomes, cellular imaging, cellular uptake

  9. The cellular story of dishevelleds.

    Science.gov (United States)

    Kafka, Anja; Bašić-Kinda, Sandra; Pećina-Šlaus, Nives

    2014-10-01

    Dishevelled (DVL) proteins, three of which have been identified in humans, are highly conserved components of canonical and noncanonical Wnt signaling pathways. These multifunctional proteins, originally discovered in the fruit fly, through their different domains mediate complex signal transduction: DIX (dishevelled, axin) and PDZ (postsynaptic density 95, discs large, zonula occludens-1) domains serve for canonical beta-catenin signaling, while PDZ and DEP (dishevelled, Egl-10, pleckstrin) domains serve for non-canonical signaling. In canonical or beta-catenin signaling, DVL forms large molecular supercomplexes at the plasma membrane consisting of Wnt-Fz-LRP5/6-DVL-AXIN. This promotes the disassembly of the beta-catenin destruction machinery, beta-catenin accumulation, and consequent activation of Wnt signaling. Therefore, DVLs are considered to be key regulators that rescue cytoplasmic beta-catenin from degradation. The potential medical importance of DVLs is in both human degenerative disease and cancer. The overexpression of DVL has been shown to potentiate the activation of Wnt signaling and it is now apparent that up-regulation of DVLs is involved in several types of cancer.

  10. Instrumentos de observación ad hoc para el análisis de las acciones motrices en Danza Contemporánea, Expresión Corporal y Danza Contact-Improvisation

    Directory of Open Access Journals (Sweden)

    Marta Castañer Balcells

    2009-03-01

    Full Text Available La Danza y la Expresión Corporal son disciplinas que promueven la continua generación de acciones motrices diversas y singularizadas y es por ello que se reclama la creación de sistemas de categorías específicos para su observación y análisis. En este artículo exponemos tres estudios que nos han permitido elaborar, mediante la metodología observacional, tres sistemas de categorías de un modo progresivo y ad hoc a estas disciplinas. Los tres forman parte de una investigación institucional de la AGAUR (INEFCP. Si el objeto de estudio es la capacidad de generar respuestas motrices singularizadas, las dimensiones, según la especificidad de cada sistema, están estructuradas desde tres niveles de análisis: el primero con relación a las fases de todo proceso creativo (Guildford, 1970; el segundo con relación a las habilidades motrices a partir del sistema de observación OSMOS (Castañer, Torrents, Dinušová, Anguera, 2008, y, el tercero con relación a las dimensiones de los contextos naturales (Anguera, 2005 y, en concreto de la Danza (Laban, 1988. La codificación se ha realizado mediante el software Match Vision Studio (Perea, Ezpeleta, Castellano, 2004.

  11. Unified Stochastic Geometry Model for MIMO Cellular Networks with Retransmissions

    KAUST Repository

    Afify, Laila H.

    2016-10-11

    This paper presents a unified mathematical paradigm, based on stochastic geometry, for downlink cellular networks with multiple-input-multiple-output (MIMO) base stations (BSs). The developed paradigm accounts for signal retransmission upon decoding errors, in which the temporal correlation among the signal-to-interference-plus-noise-ratio (SINR) of the original and retransmitted signals is captured. In addition to modeling the effect of retransmission on the network performance, the developed mathematical model presents twofold analysis unification for MIMO cellular networks literature. First, it integrates the tangible decoding error probability and the abstracted (i.e., modulation scheme and receiver type agnostic) outage probability analysis, which are largely disjoint in the literature. Second, it unifies the analysis for different MIMO configurations. The unified MIMO analysis is achieved by abstracting unnecessary information conveyed within the interfering signals by Gaussian signaling approximation along with an equivalent SISO representation for the per-data stream SINR in MIMO cellular networks. We show that the proposed unification simplifies the analysis without sacrificing the model accuracy. To this end, we discuss the diversity-multiplexing tradeoff imposed by different MIMO schemes and shed light on the diversity loss due to the temporal correlation among the SINRs of the original and retransmitted signals. Finally, several design insights are highlighted.

  12. Pseudoislet of hybrid cellular spheroids from commercial cell lines.

    Science.gov (United States)

    Jo, Y H; Nam, B M; Kim, B Y; Nemeno, J G; Lee, S; Yeo, J E; Yang, W; Park, S H; Kim, Y S; Lee, J I

    2013-10-01

    Investigators conducting diabetes-related research have focused on islet transplantation as a radical therapy for type 1 diabetes mellitus. Pancreatic islet isolation, an essential process, is a very demanding work because of the proteolytic enzymes, species, treatment time, and individual difference. Replacement of primary isolated pancreatic islets must be carried out continuously for various in vitro tests, making primary isolated islets a useful tool for cell transplantation research. Hence, we sought to develop pseudoislets from commercial pancreas-derived cell lines. In this study, we used RIN-5F and RIN-m cells, which secrete insulin, somatostatin, or glucagon. To manufacture hybrid cellular spheroids, the cells were cultured under hanging drop plate and nonadhesive plate methods. We observed that hybrid cellular pseudoislets exhibited an oval shape, with sizes ranging from 590 to 1200 μm. Their morphology was similar to naïve islets. Cell line pseudoislets secreted and expressed insulin, glucagon, and somatostatin, as confirmed by reverse transcriptase polymerase chain reaction, enzyme-linked immunosorbent assay, and immunohistochemistry analyses. Thus, the current artificially manufactured biomimetic pseudoislets resembled pancreatic islets of the endocrine system, appearing as cellular aggregates that secreted insulin, glucagon, and somatostatin. Enhanced immunoisolation techniques may lead to the development of new islet sources for pancreatic transplantation through this pseudoislet strategy.

  13. HIV-1 Replication and the Cellular Eukaryotic Translation Apparatus

    Directory of Open Access Journals (Sweden)

    Santiago Guerrero

    2015-01-01

    Full Text Available Eukaryotic translation is a complex process composed of three main steps: initiation, elongation, and termination. During infections by RNA- and DNA-viruses, the eukaryotic translation machinery is used to assure optimal viral protein synthesis. Human immunodeficiency virus type I (HIV-1 uses several non-canonical pathways to translate its own proteins, such as leaky scanning, frameshifting, shunt, and cap-independent mechanisms. Moreover, HIV-1 modulates the host translation machinery by targeting key translation factors and overcomes different cellular obstacles that affect protein translation. In this review, we describe how HIV-1 proteins target several components of the eukaryotic translation machinery, which consequently improves viral translation and replication.

  14. HIV-1 replication and the cellular eukaryotic translation apparatus.

    Science.gov (United States)

    Guerrero, Santiago; Batisse, Julien; Libre, Camille; Bernacchi, Serena; Marquet, Roland; Paillart, Jean-Christophe

    2015-01-01

    Eukaryotic translation is a complex process composed of three main steps: initiation, elongation, and termination. During infections by RNA- and DNA-viruses, the eukaryotic translation machinery is used to assure optimal viral protein synthesis. Human immunodeficiency virus type I (HIV-1) uses several non-canonical pathways to translate its own proteins, such as leaky scanning, frameshifting, shunt, and cap-independent mechanisms. Moreover, HIV-1 modulates the host translation machinery by targeting key translation factors and overcomes different cellular obstacles that affect protein translation. In this review, we describe how HIV-1 proteins target several components of the eukaryotic translation machinery, which consequently improves viral translation and replication. PMID:25606970

  15. Cellular reprogramming for understanding and treating human disease.

    Directory of Open Access Journals (Sweden)

    Riya Rajan Kanherkar

    2014-11-01

    Full Text Available In the last two decades we have witnessed a paradigm shift in our understanding of cells so radical that it has rewritten the rules of biology. The study of cellular reprogramming has gone from little more than a hypothesis, to applied bioengineering, with the creation of a variety of important cell types. By way of metaphor, we can compare the discovery of reprogramming with the archaeological discovery of the Rosetta stone. This stone slab made possible the initial decipherment of Egyptian hieroglyphics because it allowed us to see this language in a way that was previously impossible. We propose that cellular reprogramming will have an equally profound impact on understanding and curing human disease, because it allows us to perceive and study molecular biological processes such as differentiation, epigenetics, and chromatin in ways that were likewise previously impossible. Stem cells could be called cellular Rosetta stones because they allow also us to perceive the connections between development, disease, cancer, aging, and regeneration in novel ways. Here we present a comprehensive historical review of stem cells and cellular reprogramming, and illustrate the developing synergy between many previously unconnected fields. We show how stem cells can be used to create in vitro models of human disease and provide examples of how reprogramming is being used to study and treat such diverse diseases as cancer, aging and accelerated aging syndromes, infectious diseases such as AIDS, and epigenetic diseases such as polycystic ovary syndrome. While the technology of reprogramming is being developed and refined there have also been significant ongoing developments in other complementary technologies such as gene editing, progenitor cell production, and tissue engineering. These technologies are the foundations of what is becoming a fully-functional field of regenerative medicine and are converging to a point that will allow us to treat almost any

  16. Shape Memory Alloy-Based Periodic Cellular Structures Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This SBIR Phase I effort will develop and demonstrate an innovative shape memory alloy (SMA) periodic cellular structural technology. Periodic cellular structures...

  17. Characterizing heterogeneous cellular responses to perturbations.

    Science.gov (United States)

    Slack, Michael D; Martinez, Elisabeth D; Wu, Lani F; Altschuler, Steven J

    2008-12-01

    Cellular populations have been widely observed to respond heterogeneously to perturbation. However, interpreting the observed heterogeneity is an extremely challenging problem because of the complexity of possible cellular phenotypes, the large dimension of potential perturbations, and the lack of methods for separating meaningful biological information from noise. Here, we develop an image-based approach to characterize cellular phenotypes based on patterns of signaling marker colocalization. Heterogeneous cellular populations are characterized as mixtures of phenotypically distinct subpopulations, and responses to perturbations are summarized succinctly as probabilistic redistributions of these mixtures. We apply our method to characterize the heterogeneous responses of cancer cells to a panel of drugs. We find that cells treated with drugs of (dis-)similar mechanism exhibit (dis-)similar patterns of heterogeneity. Despite the observed phenotypic diversity of cells observed within our data, low-complexity models of heterogeneity were sufficient to distinguish most classes of drug mechanism. Our approach offers a computational framework for assessing the complexity of cellular heterogeneity, investigating the degree to which perturbations induce redistributions of a limited, but nontrivial, repertoire of underlying states and revealing functional significance contained within distinct patterns of heterogeneous responses.

  18. Cellular Taxonomy of the Mouse Striatum as Revealed by Single-Cell RNA-Seq.

    Science.gov (United States)

    Gokce, Ozgun; Stanley, Geoffrey M; Treutlein, Barbara; Neff, Norma F; Camp, J Gray; Malenka, Robert C; Rothwell, Patrick E; Fuccillo, Marc V; Südhof, Thomas C; Quake, Stephen R

    2016-07-26

    The striatum contributes to many cognitive processes and disorders, but its cell types are incompletely characterized. We show that microfluidic and FACS-based single-cell RNA sequencing of mouse striatum provides a well-resolved classification of striatal cell type diversity. Transcriptome analysis revealed ten differentiated, distinct cell types, including neurons, astrocytes, oligodendrocytes, ependymal, immune, and vascular cells, and enabled the discovery of numerous marker genes. Furthermore, we identified two discrete subtypes of medium spiny neurons (MSNs) that have specific markers and that overexpress genes linked to cognitive disorders and addiction. We also describe continuous cellular identities, which increase heterogeneity within discrete cell types. Finally, we identified cell type-specific transcription and splicing factors that shape cellular identities by regulating splicing and expression patterns. Our findings suggest that functional diversity within a complex tissue arises from a small number of discrete cell types, which can exist in a continuous spectrum of functional states. PMID:27425622

  19. Cellular Taxonomy of the Mouse Striatum as Revealed by Single-Cell RNA-Seq

    Directory of Open Access Journals (Sweden)

    Ozgun Gokce

    2016-07-01

    Full Text Available The striatum contributes to many cognitive processes and disorders, but its cell types are incompletely characterized. We show that microfluidic and FACS-based single-cell RNA sequencing of mouse striatum provides a well-resolved classification of striatal cell type diversity. Transcriptome analysis revealed ten differentiated, distinct cell types, including neurons, astrocytes, oligodendrocytes, ependymal, immune, and vascular cells, and enabled the discovery of numerous marker genes. Furthermore, we identified two discrete subtypes of medium spiny neurons (MSNs that have specific markers and that overexpress genes linked to cognitive disorders and addiction. We also describe continuous cellular identities, which increase heterogeneity within discrete cell types. Finally, we identified cell type-specific transcription and splicing factors that shape cellular identities by regulating splicing and expression patterns. Our findings suggest that functional diversity within a complex tissue arises from a small number of discrete cell types, which can exist in a continuous spectrum of functional states.

  20. Parametric study of double cellular detonation structure

    Science.gov (United States)

    Khasainov, B.; Virot, F.; Presles, H.-N.; Desbordes, D.

    2013-05-01

    A parametric numerical study is performed of a detonation cellular structure in a model gaseous explosive mixture whose decomposition occurs in two successive exothermic reaction steps with markedly different characteristic times. Kinetic and energetic parameters of both reactions are varied in a wide range in the case of one-dimensional steady and two-dimensional (2D) quasi-steady self-supported detonations. The range of governing parameters of both exothermic steps is defined where a "marked" double cellular structure exists. It is shown that the two-level cellular structure is completely governed by the kinetic parameters and the local overdrive ratio of the detonation front propagating inside large cells. Furthermore, since it is quite cumbersome to use detailed chemical kinetics in unsteady 2D case, the proposed work should help to identify the mixtures and the domain of their equivalence ratio where double detonation structure could be observed.

  1. Infrared image enhancement using Cellular Automata

    Science.gov (United States)

    Qi, Wei; Han, Jing; Zhang, Yi; Bai, Lian-fa

    2016-05-01

    Image enhancement is a crucial technique for infrared images. The clear image details are important for improving the quality of infrared images in computer vision. In this paper, we propose a new enhancement method based on two priors via Cellular Automata. First, we directly learn the gradient distribution prior from the images via Cellular Automata. Second, considering the importance of image details, we propose a new gradient distribution error to encode the structure information via Cellular Automata. Finally, an iterative method is applied to remap the original image based on two priors, further improving the quality of enhanced image. Our method is simple in implementation, easy to understand, extensible to accommodate other vision tasks, and produces more accurate results. Experiments show that the proposed method performs better than other methods using qualitative and quantitative measures.

  2. Spin Echo Studies on Cellular Water

    CERN Document Server

    Chang, D C; Nichols, B L; Rorschach, H E

    2014-01-01

    Previous studies indicated that the physical state of cellular water could be significantly different from pure liquid water. To experimentally investigate this possibility, we conducted a series of spin-echo NMR measurements on water protons in rat skeletal muscle. Our result indicated that the spin-lattice relaxation time and the spin-spin relaxation time of cellular water protons are both significantly shorter than that of pure water (by 4.3-fold and 34-fold, respectively). Furthermore, the spin diffusion coefficient of water proton is almost 1/2 of that of pure water. These data suggest that cellular water is in a more ordered state in comparison to pure water.

  3. Online isolation of defects in cellular nanocomputers

    Institute of Scientific and Technical Information of China (English)

    Teijiro Isokawa; Shin'ya Kowada; Ferdinand Peper; Naotake Kamiura; Nobuyuki Matsui

    2007-01-01

    Unreliability will be a major issue for computers built from components at nanometer scales.Thus,it's to be expected that such computers will need a high degree of defect-tolerance to overcome components' defects which have arisen during the process of manufacturing.This paper presents a novel approach to defect-tolerance that is especially geared towards nanocomputers based on asynchronous cellular automata.According to this approach,defective cells are detected and isolated by small configurations that move around randomly in cellular space.These configurations,called random flies,will attach to configurations that are static,which is typical for configurations that contain defective cells.On the other hand,dynamic configurations,like those that conduct computations,will not be isolated from the rest of the cellular space by the random flies,and will be able to continue their operations unaffectedly.

  4. Software-Defined Cellular Mobile Network Solutions

    Institute of Scientific and Technical Information of China (English)

    Jiandong Li; Peng Liu; Hongyan Li

    2014-01-01

    The emergency relating to software-defined networking (SDN), especially in terms of the prototype associated with OpenFlow, pro-vides new possibilities for innovating on network design. Researchers have started to extend SDN to cellular networks. Such new programmable architecture is beneficial to the evolution of mobile networks and allows operators to provide better services. The typical cellular network comprises radio access network (RAN) and core network (CN); hence, the technique roadmap diverges in two ways. In this paper, we investigate SoftRAN, the latest SDN solution for RAN, and SoftCell and MobileFlow, the latest solu-tions for CN. We also define a series of control functions for CROWD. Unlike in the other literature, we emphasize only software-defined cellular network solutions and specifications in order to provide possible research directions.

  5. Asymptotic Behavior of Excitable Cellular Automata

    CERN Document Server

    Durrett, R; Durrett, Richard; Griffeath, David

    1993-01-01

    Abstract: We study two families of excitable cellular automata known as the Greenberg-Hastings Model (GHM) and the Cyclic Cellular Automaton (CCA). Each family consists of local deterministic oscillating lattice dynamics, with parallel discrete-time updating, parametrized by the range of interaction, the "shape" of its neighbor set, threshold value for contact updating, and number of possible states per site. GHM and CCA are mathematically tractable prototypes for the spatially distributed periodic wave activity of so-called excitable media observed in diverse disciplines of experimental science. Earlier work by Fisch, Gravner, and Griffeath studied the ergodic behavior of these excitable cellular automata on Z^2, and identified two distinct (but closely-related) elaborate phase portraits as the parameters vary. In particular, they noted the emergence of asymptotic phase diagrams (and Euclidean dynamics) in a well-defined threshold-range scaling limit. In this study we present several rigorous results and som...

  6. Alleviate Cellular Congestion Through Opportunistic Trough Filling

    Directory of Open Access Journals (Sweden)

    Yichuan Wang

    2014-04-01

    Full Text Available The demand for cellular data service has been skyrocketing since the debut of data-intensive smart phones and touchpads. However, not all data are created equal. Many popular applications on mobile devices, such as email synchronization and social network updates, are delay tolerant. In addition, cellular load varies significantly in both large and small time scales. To alleviate network congestion and improve network performance, we present a set of opportunistic trough filling schemes that leverage the time-variation of network congestion and delay-tolerance of certain traffic in this paper. We consider average delay, deadline, and clearance time as the performance metrics. Simulation results show promising performance improvement over the standard schemes. The work shed lights on addressing the pressing issue of cellular overload.

  7. Cellular automatons applied to gas dynamic problems

    Science.gov (United States)

    Long, Lyle N.; Coopersmith, Robert M.; Mclachlan, B. G.

    1987-01-01

    This paper compares the results of a relatively new computational fluid dynamics method, cellular automatons, with experimental data and analytical results. This technique has been shown to qualitatively predict fluidlike behavior; however, there have been few published comparisons with experiment or other theories. Comparisons are made for a one-dimensional supersonic piston problem, Stokes first problem, and the flow past a normal flat plate. These comparisons are used to assess the ability of the method to accurately model fluid dynamic behavior and to point out its limitations. Reasonable results were obtained for all three test cases, but the fundamental limitations of cellular automatons are numerous. It may be misleading, at this time, to say that cellular automatons are a computationally efficient technique. Other methods, based on continuum or kinetic theory, would also be very efficient if as little of the physics were included.

  8. Characterization of cellular titanium for biomedical applications

    Science.gov (United States)

    Hrabe, Nikolas Wilson

    By controlling structural features (relative density, pore size, strut size) of cellular titanium (also known as porous titanium), the mechanical properties can be optimized to reduce the effects of stress shielding currently observed in load-bearing bone replacement implants. Thermal gravimetric analysis of a sacrificial scaffold system lead to important processing modifications in an attempt to meet chemistry requirements for surgical grade titanium not met in previous work. Despite these modifications chemistry did not meet requirements for carbon, nitrogen, or oxygen. Commercially pure titanium (CPTi) porous structures were made over a range of relative densities using laser engineered net shaping (LENS). From monotonic compression tests, yield strength and elastic modulus in the range of bone were achieved but did not scale with relative density as predicted by the Gibson-Ashby analytical model. Compression-compression fatigue resistance was high, as no failures were observed for test stresses up to 133% yield strength, which is thought to be influenced by the dense exterior shell of the samples. Structures were also fabricated over a range of relative densities using selective electron beam melting (SEBM or EBM), and structural, mechanical, and in-vitro properties were measured for three materials (as-built Ti-6A1-4V, Ti-6A1-4V after hot isostatic pressing (HIPing), and as-built CPTi). For structures of all three materials, yield strength and elastic modulus was within the range for bone. Numerical modeling results suggested cell shape and sintered particles on strut surfaces affect the scaling of elastic modulus with relative density and lead to the observed difference from the Gibson-Ashby model. Normalized fatigue strengths at 106 cycles ranged from 0.150.25 for as-built Ti-6A1-4V structures, which is lower than expected. Results for HIPed Ti-6A1-4V structures and CPTi structures suggest that stress concentrations from closed porosity within struts as well

  9. Cellular injury evidenced by impedance technology and infrared microspectroscopy

    Science.gov (United States)

    le Roux, K.; Prinsloo, L. C.; Meyer, D.

    2015-03-01

    Fourier Transform Infrared (FTIR) spectroscopy is finding increasing biological application, for example in the analysis of diseased tissues and cells, cell cycle studies and investigating the mechanisms of action of anticancer drugs. Cancer treatment studies routinely define the types of cell-drug responses as either total cell destruction by the drug (all cells die), moderate damage (cell deterioration where some cells survive) or reversible cell cycle arrest (cytostasis). In this study the loss of viability and related chemical stress experienced by cells treated with the medicinal plant, Plectranthus ciliatus, was investigated using real time cell electronic sensing (RT-CES) technology and FTIR microspectroscopy. The use of plants as medicines is well established and ethnobotany has proven that crude extracts can serve as treatments against various ailments. The aim of this study was to determine whether FTIR microspectroscopy would successfully distinguish between different types of cellular injury induced by a potentially anticancerous plant extract. Cervical adenocarcinoma (HeLa) cells were treated with a crude extract of Pciliatus and cells monitored using RT-CES to characterize the type of cellular responses induced. Cell populations were then investigated using FTIR microspectroscopy and statistically analysed using One-way Analysis of Variance (ANOVA) and Principal Component Analysis (PCA). The plant extract and a cancer drug control (actinomycin D) induced concentration dependent cellular responses ranging from nontoxic, cytostatic or cytotoxic. Thirteen spectral peaks (915 cm-1, 933 cm-1, 989 cm-1, 1192 cm-1, 1369 cm-1, 1437 cm-1, 1450 cm-1, 1546 cm-1, 1634 cm-1, 1679 cm-1 1772 cm-1, 2874 cm-1 and 2962 cm-1) associated with cytotoxicity were significantly (p value < 0.05, one way ANOVA, Tukey test, Bonferroni) altered, while two of the bands were also indicative of early stress related responses. In PCA, poor separation between nontoxic and cytostatic

  10. Inhibitory action of chlorophyllin of autosome recessive lethals induced by irradiation; Accion inhibidora de la clorofilina de letales recesivos autosonicos inducidos por irradiacion

    Energy Technology Data Exchange (ETDEWEB)

    Salceda, V.M.; Pimentel, P.A.E.; Cruces, M.P. [ININ, 52045 Ocoyoacac, Estado de Mexico (Mexico)]. e-mail: vmss@nuclear.inin.mx

    2006-07-01

    chlorophyllin on the damage caused by the radiation, it was into accothe presence of lethal and semi lethals autosomal. One observes this way that even without the use of the radiation the semi lethals frequency is diminished when the chlorophyllin is applied, in this case the decrease was significant and although there was decrease in the case of the irradiated group this it was not significant; in the case of the lethal ones it happened the opposite it was not significant in radiation absence on the contrary elevate the frequency of this type of genes, however, before the radiation and with pre-treatment with chlorophyllin this it reduced the frequency of autosomal recessive lethals significantly. This is important because in the case of bound recessive lethals recessive to the sex this doesn't happen. (Author)

  11. Toxicology and cellular effect of manufactured nanomaterials

    Science.gov (United States)

    Chen, Fanqing

    2014-07-22

    The increasing use of nanotechnology in consumer products and medical applications underlies the importance of understanding its potential toxic effects to people and the environment. Herein are described methods and assays to predict and evaluate the cellular effects of nanomaterial exposure. Exposing cells to nanomaterials at cytotoxic doses induces cell cycle arrest and increases apoptosis/necrosis, activates genes involved in cellular transport, metabolism, cell cycle regulation, and stress response. Certain nanomaterials induce genes indicative of a strong immune and inflammatory response within skin fibroblasts. Furthermore, the described multiwall carbon nanoonions (MWCNOs) can be used as a therapeutic in the treatment of cancer due to its cytotoxicity.

  12. The cellular decision between apoptosis and autophagy

    Institute of Scientific and Technical Information of China (English)

    Yong-Jun Fan; Wei-Xing Zong

    2013-01-01

    Apoptosis and autophagy are important molecular processes that maintain organismal and cellular homeostasis,respectively.While apoptosis fulfills its role through dismantling damaged or unwanted cells,autophagy maintains cellular homeostasis through recycling selective intracellular organelles and molecules.Yet in some conditions,autophagy can lead to cell death.Apoptosis and autophagy can be stimulated by the same stresses.Emerging evidence indicates an interplay between the core proteins in both pathways,which underlies the molecular mechanism of the crosstalk between apoptosis and autophagy.This review summarizes recent literature on molecules that regulate both the apoptotic and autophagic processes.

  13. Rapid Cellular Turnover in Adipose Tissue

    OpenAIRE

    Alessandra Rigamonti; Kristen Brennand; Frank Lau; Cowan, Chad A.

    2011-01-01

    It was recently shown that cellular turnover occurs within the human adipocyte population. Through three independent experimental approaches — dilution of an inducible histone 2B-green fluorescent protein (H2BGFP), labeling with the cell cycle marker Ki67 and incorporation of BrdU — we characterized the degree of cellular turnover in murine adipose tissue. We observed rapid turnover of the adipocyte population, finding that 4.8% of preadipocytes are replicating at any time and that between 1–...

  14. Chaotic behavior in the disorder cellular automata

    International Nuclear Information System (INIS)

    Disordered cellular automata (DCA) represent an intermediate class between elementary cellular automata and the Kauffman network. Recently, Rule 126 of DCA has been explicated: the system can be accurately described by a discrete probability function. However, a means of extending to other rules has not been developed. In this investigation, a density map of the dynamical behavior of DCA is formulated based on Rule 22 and other totalistic rules. The numerical results reveal excellent agreement between the model and original automata. Furthermore, the inhomogeneous situation is also discussed

  15. Green Cellular - Optimizing the Cellular Network for Minimal Emission from Mobile Stations

    CERN Document Server

    Ezri, Doron

    2009-01-01

    Wireless systems, which include cellular phones, have become an essential part of the modern life. However the mounting evidence that cellular radiation might adversely affect the health of its users, leads to a growing concern among authorities and the general public. Radiating antennas in the proximity of the user, such as antennas of mobile phones are of special interest for this matter. In this paper we suggest a new architecture for wireless networks, aiming at minimal emission from mobile stations, without any additional radiation sources. The new architecture, dubbed Green Cellular, abandons the classical transceiver base station design and suggests the augmentation of transceiver base stations with receive only devices. These devices, dubbed Green Antennas, are not aiming at coverage extension but rather at minimizing the emission from mobile stations. We discuss the implications of the Green Cellular architecture on 3G and 4G cellular technologies. We conclude by showing that employing the Green Cell...

  16. Muscle biopsies off-set normal cellular signaling in surrounding musculature

    DEFF Research Database (Denmark)

    Krag, Thomas O; Hauerslev, Simon; Dahlqvist, Julia R;

    2013-01-01

    Studies of muscle physiology and muscular disorders often require muscle biopsies to answer questions about muscle biology. In this context, we have often wondered if muscle biopsies, especially if performed repeatedly, would affect interpretation of muscle morphology and cellular signaling. We...... hypothesized that muscle morphology and cellular signaling involved in myogenesis/regeneration and protein turnover can be changed by a previous muscle biopsy in close proximity to the area under investigation. Here we report a case where a past biopsy or biopsies affect cellular signaling of the surrounding...... muscle tissue for at least 3 weeks after the biopsy was performed and magnetic resonance imaging suggests that an effect of a biopsy may persist for at least 5 months. Cellular signaling after a biopsy resembles what is seen in severe limb-girdle muscular dystrophy type 2I with respect to protein...

  17. Influence of microgravity on cellular differentiation in root caps of Zea mays

    Science.gov (United States)

    Moore, R.; Fondren, W. M.; McClelen, C. E.; Wang, C. L.

    1987-01-01

    We launched imbibed seeds of Zea mays into outer space aboard the space shuttle Columbia to determine the influence of microgravity on cellular differentiation in root caps. The influence of microgravity varied with different stages of cellular differentiation. Overall, microgravity tended to 1) increase relative volumes of hyaloplasm and lipid bodies, 2) decrease the relative volumes of plastids, mitochondria, dictyosomes, and the vacuome, and 3) exert no influence on the relative volume of nuclei in cells comprising the root cap. The reduced allocation of dictyosomal volume in peripheral cells of flight-grown seedlings correlated positively with their secretion of significantly less mucilage than peripheral cells of Earth-grown seedlings. These results indicate that 1) microgravity alters the patterns of cellular differentiation and structures of all cell types comprising the root cap, and 2) the influence of microgravity on cellular differentiation in root caps of Zea mays is organelle specific.

  18. Energy conservation and contribution to the sustained development through action in the bioclimate design; Ahorro de energia y contribucion al desarrollo sustentable a partir de acciones de diseno bioclimatico

    Energy Technology Data Exchange (ETDEWEB)

    Garcia Chavez, J. R.; Dorantes Rodriguez, R. [Universidad Autonoma Metropolitana, Unidad Azcapotzalco, Mexico, D. F. (Mexico)

    1995-12-31

    , construction and monitoring of the functioning of cooling passive systems. The results obtained to date point out that the energy consumption has diminished and improved the environmental comfort conditions for the library building users. [Espanol] La crisis mundial de energia de 1973, ademas de los problemas de abastecimiento y elevacion de precios, propicio tambien diversos paises, una reaccion orientada al uso eficiente y conservacion de la energia, y a la aplicacion de una arquitectura con sentido comun: la ARQUITECTURA BIOCLIMATICA, misma que se basa en la utilizacion en las edificaciones de las energias alternativas, tales como la solar eolica, etc. Obtencion de condiciones de confort natural ambiental y ahorro y uso eficiente de la energia en los edificios, son los objetivos fundamentales de la ARQUITECTURA BIOCLIMATICA. Bajo estas consideraciones, se han planteado diversos sistemas pasivos para control termico de edificios, los cuales se han aplicado en diferentes regiones climaticas del planeta. En el presente trabajo, se presenta y analiza el potencial de medidas para ahorro de energia, tales como sistemas de iluminacion artificial de alta eficiencia y sistemas pasivos de enfriamiento para el control termico de un edificio educativo, la biblioteca central de la Universidad Autonoma Metropolitana, Unidad Azcapotzalco, ubicada en la ciudad de Mexico. El objetivo de este estudio se orienta a la reduccion del consumo de energia en la biblioteca, y a la obtencion de condiciones de confort ambiental higrotermico para sus ocupantes. Estudios realizados durante la primera etapa de investigacion, han permitido identificar un excesivo consumo de energia y condiciones de falta de confort higrotermico y de la necesaria calidad del aire interior en la biblioteca. Estas acciones se han enfocado en dos aspectos. El primero se refiere al mejoramiento de la operacion de la instalacion electrica y el alumbrado de la biblioteca. Estas acciones se basaron en la sustitucion de lamparas

  19. Ações de cuidado desempenhadas pelo pai no puerpério Acciones del cuidados desempeñados por el papá durante el puerperio Actions in care carried out by the father in the puerperism

    Directory of Open Access Journals (Sweden)

    Eteniger Marcela Fernandes de Oliveira

    2009-09-01

    Full Text Available Pesquisa exploratória-descritiva, qualitativa, desenvolvida com 15 pais, de janeiro a abril de 2006, em Natal/RN. Teve por objetivo analisar as ações desenvolvidas pelo homem durante o puerpério de sua companheira. A coleta efetuou-se por entrevista semiestruturada, sendo os dados tratados por meio da técnica de análise de conteúdo e analisados à luz dos princípios do Interacionismo Simbólico. Os resultados apresentam uma temática central - ações de cuidado em ambiente doméstico - e duas categorias: cuidando da companheira e da criança; e provendo o sustento da família. Conclui-se que os depoentes interagem e participam do puerpério oferecendo conselhos que remetem à prevenção, como o estímulo à deambulação; desenvolvem atitudes de dedicação e preocupação com a saúde do filho; consideram que a licença-paternidade não atende às suas necessidades; reconhecem que a companheira e o recém-nascido requerem mais dedicação e que desempenham papel de provedor no intuito de garantir o sustento familiar.Investigación exploratoria y descriptiva, cualitativa, desarrollada con 15 padres de familia, en Natal/RN. La pesquisa analizó las acciones desarrolladas por el papá durante el puerperio de su compañera. Los datos fueron recolectados por medio de la entrevista semiestructurada, organizados por la técnica de análisis de contenido y analizados bajo los principios del Interaccionismo Simbólico. Los resultados mostraron que existe un tema central las acciones de atención yl cuidado en el ambiente doméstico - y dos categorías cuidar de la pareja y del niño, sin dejar de lado el sustento de la familia. Se concluyó que estos padres conviven durante el puerperio ofreciendo consejos que remiten a la prevención, como estimular a la mujer a hacer caminadas; desarrollan actitudes de dedicación y preocupación con la salud del hijo; consideran que el permiso de trabajo por paternidad no contempla sus necesidades

  20. Actual trends in public relations involving in public political institutions/ Tendencias de las acciones de RRPP desarrolladas en instituciones públicas de carácter político

    Directory of Open Access Journals (Sweden)

    Dra. Ana Belén Fernández Souto; abfsouto@uvigo.es

    2009-01-01

    Full Text Available In the next pages, we’ll try to study the work of public relations in political institutions. To do this, we’ll revise a lot of definitions of this concept, besides their instruments, tactics and strategies they use to get their objectives and their actual trends. This is a very interesting subject but not very worked in public relations, as in political marketing or political communication, so we’ll try to do practical contributions. We’ll work with de hypothesis that the play of public relations is very important in the political institutions, because in a lot of times, they become as the basis of their political and propaganda advertisements.A lo largo de las siguientes páginas procuraremos abordar someramente el trabajo de las relaciones públicas aplicadas al campo institucional de ámbito político. Para ello nos remitiremos a las miles de definiciones existentes para este concepto, así como a los instrumentos, tácticas y estrategias de las que se sirven para alcanzar sus objetivos y sus actuales tendencias. Se trata de un tema relativamente poco estudiado desde el punto de vista de la comunicación organizacional y más desde el marketing político y de la comunicación política, por lo que procuraremos realizar aportaciones prácticas en este sentido. Por lo tanto, partiremos de la hipótesis de las acciones de relaciones públicas juegan un papel más que destacado en la actividad institucional de carácter político, llegando –en algunos casos– a constituirse en el verdadero leivmotiv y base de las campañas políticas y propagandísticas, así como en el principal eje comunicativo de muchas campañas institucionales.

  1. Action of the surge in the stability of the marine bed in the sounding of Campeche, Mexico; Accion del oleaje en la estabilidad del lecho marino en la sonda de Campeche, Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Mendoza Baldwin, E.G.; Silva Casarin, R. [Instituto de Ingenieria, UNAM, (Mexico); Salazar Carrillo, E.E. [Instituto Mexicano del Petroleo (Mexico)

    2004-12-01

    Among the phenomena that must be taken into account while designing and constructing coastal structures, the wave-induced soil response has become a very important issue. One reason for this growing interest is that many structures have been reportedly failed because of seabed instability rather than design or construction deficiencies. In this paper we present the application to Sonda de Campeche, Mexico of an analytical model for a porous seabed of finite thickness, h, subject a 3D short crested wave system. This exact solution gives explicit expressions for the pore pressure and effective stresses under. [Spanish] La seguridad de las actividades relacionadas con el litoral depende, en gran medida, del correcto funcionamiento de las estructuras de operacion y proteccion, el diseno de las cuales, a su vez, requiere de un conocimiento lo mas completo posible, tanto de las solicitaciones como del comportamiento del entorno en el que seran construidas. Como respuesta a dichas necesidades, en este trabajo se presenta la aplicacion de un modelo en tres dimensiones con el que se puede representar la respuesta del lecho marino, considerando la existencia de un estrato poroso de espesor finito ante la accion de un sistema de ondas progresivas de cresta corta, el cual es de especial utilidad para el diseno y tendido de lineas submarinas. Dentro de las bondades del modelo se cuenta con la posibilidad de varias las condiciones del tipo de suelo (cohesivo, arenoso o mixto), grado de saturacion, angulo de incidencia del oleaje y espesor del estrato poroso. El modelo tridimensional es alimentado con datos de oleaje y tipo de suelo de la bahia de Campeche, Mexico, con los que se analizan las distribuciones verticales de los esfuerzos y la presion de poro inducidos. Finalmente, se realiza un estudio de inestabilidad del lecho marino para la misma bahia, comparando los resultados del modelo con los de sondeos en campo.

  2. GABAergic Interneurons in the Neocortex: From Cellular Properties to Circuits.

    Science.gov (United States)

    Tremblay, Robin; Lee, Soohyun; Rudy, Bernardo

    2016-07-20

    Cortical networks are composed of glutamatergic excitatory projection neurons and local GABAergic inhibitory interneurons that gate signal flow and sculpt network dynamics. Although they represent a minority of the total neocortical neuronal population, GABAergic interneurons are highly heterogeneous, forming functional classes based on their morphological, electrophysiological, and molecular features, as well as connectivity and in vivo patterns of activity. Here we review our current understanding of neocortical interneuron diversity and the properties that distinguish cell types. We then discuss how the involvement of multiple cell types, each with a specific set of cellular properties, plays a crucial role in diversifying and increasing the computational power of a relatively small number of simple circuit motifs forming cortical networks. We illustrate how recent advances in the field have shed light onto the mechanisms by which GABAergic inhibition contributes to network operations. PMID:27477017

  3. On reversibility of cellular automata with periodic boundary conditions

    Energy Technology Data Exchange (ETDEWEB)

    Nobe, Atsushi [Graduate School of Engineering Science, Osaka University, Machikaneyama-cho 1-3, Toyonaka, Osaka 560-8531 (Japan); Yura, Fumitaka [Imai Quantum Computing and Information Project, ERATO, JST, Daini Hongo White Bldg 201, 5-28-3 Hongo, Bunkyo, Tokyo 113-0033 (Japan)

    2004-06-04

    Reversibility of one-dimensional cellular automata with periodic boundary conditions is discussed. It is shown that there exist exactly 16 reversible elementary cellular automaton rules for infinitely many cell sizes by means of a correspondence between elementary cellular automaton and the de Bruijn graph. In addition, a sufficient condition for reversibility of three-valued and two-neighbour cellular automaton is given.

  4. Cellular Automata Rules and Linear Numbers

    OpenAIRE

    Nayak, Birendra Kumar; Sahoo, Sudhakar; Biswal, Sagarika

    2012-01-01

    In this paper, linear Cellular Automta (CA) rules are recursively generated using a binary tree rooted at "0". Some mathematical results on linear as well as non-linear CA rules are derived. Integers associated with linear CA rules are defined as linear numbers and the properties of these linear numbers are studied.

  5. Virtual cellular manufacturing : Configuring routing flexibility

    NARCIS (Netherlands)

    Nomden, G.; van der Zee, D.J.

    2008-01-01

    Virtual cellular manufacturing (VCM) creates groups of products and machines in the production planning and control system. Similar groupings may help to reduce set-up times. Starting from two industrial cases, we study parallel machine shops assuming the implementation of VCM. We address the way mi

  6. Determining lineage pathways from cellular barcoding experiments

    NARCIS (Netherlands)

    Perié, Leïla; Hodgkin, Philip D; Naik, Shalin H; Schumacher, Ton N; de Boer, Rob J; Duffy, Ken R

    2014-01-01

    Cellular barcoding and other single-cell lineage-tracing strategies form experimental methodologies for analysis of in vivo cell fate that have been instrumental in several significant recent discoveries. Due to the highly nonlinear nature of proliferation and differentiation, interrogation of the r

  7. Building mathematics cellular phone learning communities

    Directory of Open Access Journals (Sweden)

    Wajeeh M. Daher

    2011-04-01

    Full Text Available Researchers emphasize the importance of maintaining learning communities and environments. This article describes the building and nourishment of a learning community, one comprised of middle school students who learned mathematics out-of-class using the cellular phone. The building of the learning community was led by three third year pre-service teachers majoring in mathematics and computers. The pre-service teachers selected thirty 8th grade students to learn mathematics with the cellular phone and be part of a learning community experimenting with this learning. To analyze the building and development stages of the cellular phone learning community, two models of community building stages were used; first the team development model developed by Tuckman (1965, second the life cycle model of a virtual learning community developed by Garber (2004. The research findings indicate that a learning community which is centered on a new technology has five 'life' phases of development: Pre-birth, birth, formation, performing, and maturity. Further, the research finding indicate that the norms that were encouraged by the preservice teachers who initiated the cellular phone learning community resulted in a community which developed, nourished and matured to be similar to a community of experienced applied mathematicians who use mathematical formulae to study everyday phenomena.

  8. Cellular grafts in management of leucoderma

    Directory of Open Access Journals (Sweden)

    Mysore Venkataram

    2009-01-01

    Full Text Available Cellular grafting methods constitute important advances in the surgical management of leucoderma. Different methods such as noncultured epidermal suspensions, melanocyte cultures, and melanocyte-keratinocyte cultures have all been shown to be effective. This article reviews these methods.

  9. Cellular telephone use and cancer risk

    DEFF Research Database (Denmark)

    2006-01-01

    expected in the Danish population. RESULTS: A total of 14,249 cancers were observed (SIR = 0.95; 95% confidence interval [CI] = 0.93 to 0.97) for men and women combined. Cellular telephone use was not associated with increased risk for brain tumors (SIR = 0.97), acoustic neuromas (SIR = 0.73), salivary...

  10. Binding affinity of amyloid oligomers to cellular membranes is a generic indicator of cellular dysfunction in protein misfolding diseases

    Science.gov (United States)

    Evangelisti, Elisa; Cascella, Roberta; Becatti, Matteo; Marrazza, Giovanna; Dobson, Christopher M.; Chiti, Fabrizio; Stefani, Massimo; Cecchi, Cristina

    2016-01-01

    The conversion of peptides or proteins from their soluble native states into intractable amyloid deposits is associated with a wide range of human disorders. Misfolded protein oligomers formed during the process of aggregation have been identified as the primary pathogenic agents in many such conditions. Here, we show the existence of a quantitative relationship between the degree of binding to neuronal cells of different types of oligomers formed from a model protein, HypF-N, and the GM1 content of the plasma membranes. In addition, remarkably similar behavior is observed for oligomers of the Aβ42 peptide associated with Alzheimer’s disease. Further analysis has revealed the existence of a linear correlation between the level of the influx of Ca2+ across neuronal membranes that triggers cellular damage, and the fraction of oligomeric species bound to the membrane. Our findings indicate that the susceptibility of neuronal cells to different types of misfolded oligomeric assemblies is directly related to the extent of binding of such oligomers to the cellular membrane. PMID:27619987

  11. Klotho-Dependent Cellular Transport Regulation.

    Science.gov (United States)

    Sopjani, M; Dërmaku-Sopjani, M

    2016-01-01

    Klotho is a transmembrane protein that in humans is encoded by the hKL gene. This protein is known to have aging suppressor effects and is predominantly expressed in the distal convoluted tubule of the kidney, parathyroid glands, and choroid plexus of the brain. The Klotho protein exists in both full-length membrane form and a soluble secreted form, which exerts numerous distinct functions. The extracellular domain of Klotho can be enzymatically cleaved off and released into the systemic circulation where it functions as β-glucuronidase and a hormone. Soluble Klotho is a multifunction protein present in the biological fluids including blood, urine, and cerebrospinal fluid of mammals. Klotho deficiency leads to multiple organ failure accompanied by early appearance of multiple age-related disorders and early death, whereas overexpression of Klotho results in the opposite effects. Klotho, an enzyme and hormone, has been reported to participate in the regulation of cellular transport processes across the plasma membrane either indirectly through inhibiting calcitriol (1,25(OH)2D3) formation or other mechanism, or by directly affecting transporter proteins, including ion channels, cellular carriers, and Na(+)/K(+)-ATPase. Accordingly, Klotho protein serves as a powerful regulator of cellular transport across the plasma membrane. Importantly, Klotho-dependent cellular transport regulation implies stimulatory or inhibitory effects. Klotho has been shown to play a key role in the regulation of multiple calcium and potassium ion channels, and various cellular carriers including the Na(+)-coupled cotransporters such as NaPi-IIa, NaPi-IIb, EAAT3, and EAAT4, CreaT1 as well as Na(+)/K(+)-ATPase. These regulations are parts of the antiaging function of Klotho, which will be discussing throughout this chapter. Clearly, further experimental efforts are required to investigate the effect of Klotho on other transport proteins and underlying molecular mechanisms by which Klotho

  12. Cellular senescence as the causal nexus of aging

    Directory of Open Access Journals (Sweden)

    Naina eBhatia-Dey

    2016-02-01

    Full Text Available We present cellular senescence as the ultimate driver of the aging process, as a causal nexus that bridges microscopic subcellular damage with the phenotypic, macroscopic effect of aging. It is important to understand how the various types of subcellular damage correlated with the aging process lead to the larger, visible effects of anatomical aging. While it has always been assumed that subcellular damage (cause results in macroscopic aging (effect, the bridging link between the two has been hard to define. Here, we propose that this bridge, which we term the causal nexus, is in fact cellular senescence. The subcellular damage itself does not directly cause the visible signs of aging, but rather, as the damage accumulates and reaches a critical mass, cells cease to proliferate and acquire the deleterious senescence-associated secretory phenotype (SASP which then leads to the macroscopic consequences of tissue breakdown to create the physiologically aged phenotype. Thus senescence is a precondition for anatomical aging, and this explains why aging is a gradual process that remains largely invisible during most of its progression. The subcellular damage includes shortening of telomeres, damage to mitochondria, aneuploidy and DNA double-strand breaks triggered by various genetic, epigenetic, and environmental factors. Damage pathways acting in isolation or in concert converge at the causal nexus of cellular senescence. In each species some types of damage can be more causative than in others and operate at a variable pace; for example, telomere erosion appears to be a primary cause in human cells, whereas activation of tumor suppressor genes is more causative in rodents. Such species-specific mechanisms indicate that despite different initial causes, most of aging is traced to a single convergent causal nexus: senescence. The exception is in some invertebrate species that escape senescence, and in nondividing cells such as neurons, where

  13. Label-Free Analysis of Cellular Lipid Droplet Formation by Non-Linear Microscopy

    Science.gov (United States)

    Schie, Iwan W.

    Cellular lipid droplets (LD) are cellular organelles that can be found in every cell type. Recent research indicates that cellular LD are involved in a large number of cellular metabolic functions, such as lipid metabolism, protection from lipotoxicity, protein storage and degradation, and many more. LD formation is frequently associated with adverse health effects, i.e. alcoholic and non-alcoholic fatty liver disease, diabetes type-2, as well as many cardiovascular disorders. Despite their wide presence, LDs are the least studied and most poorly understood cellular organelles. Typically, LDs are investigated using fluorescence-based techniques that require staining with exogenous fluorophores. Other techniques, e.g. biochemical assays, require the destruction of cells that prohibit the analysis of living cells. Therefore, in my thesis research I developed a novel compound fast-scanning nonlinear optical microscope equipped with the ability to also acquire Raman spectra at specific image locations. This system allows us to image label-free cellular LD formation in living cells and analyze the composition of single cellular LDs. Images can be acquired at near video-rate (˜16 frames/s). Furthermore, the system has the ability to acquire very large images of tissue of up to 7.5x15 cm2 total area by stitching together scans with dimensions of 1x1 mm2 in less than 1 minute. The system also enables the user to acquire Raman spectra from points of interest in the multiphoton images and provides chemically-specific data from sample volumes as small as 1 femtoliter. In my thesis I used this setup to determine the effects of VLDL lipolysis products on primary rat hepatocytes. By analyzing the Raman spectra and comparing the peak ratios for saturated and unsaturated fatty acid it was determined that the small cellular LD are highly saturated, while large cellular LDs contain mostly unsaturated lipids. Furthermore, I established a method to determine the specific contribution

  14. Acciones para el desarrollo de competencias investigativas

    OpenAIRE

    Ruiz Domínguez, Benito

    2012-01-01

    La reforma integral de educación tiene su fundamento en los altos índices de deserción y en la desarticulación del nivel medio superior. Los retos principales que tiene la reforma son la ampliación de la cobertura de acceso a estudios del nivel medio, el mejoramiento de la calidad en los procesos de enseñanza-aprendizaje y la búsqueda de equidad. El marco de esta reforma permite articular los programas de distintas opciones educativas en la educación media en el país y comprende una serie de ...

  15. Cellular studies and interaction mechanisms of extremely low frequency fields

    Science.gov (United States)

    Liburdy, Robert P.

    1995-01-01

    magnetic (B) field, or if combinations of static B and time-varying B fields represent an exposure metric for the cell. This question relates directly to understanding fundamental interaction mechanisms and to the development of a rationale for ELF dose threshold guidelines. The weight of experimental evidence indicates that an induced E field according to Faraday's law of induction during magnetic field exposures elicits cellular effects. An E-field-mediated interaction has interesting consequences for microdosimetry at the cellular level and is mechanistically consistent with an interaction at the cell surface, since the E field does not penetrate beyond the cell membrane. Recently, several studies have suggested that an ELF B field by itself or in combination with a static B field may elicit cellular effects. Thus in addition to E-field-mediated effects, other interaction mechanisms as yet not fully understood may operate at the cellular level; this complexity is in contrast to the case for ionizing radiation. In addition to the question of an exposure field metric, the biological state of the target cell is important in ELF interactions. Biological factors such as cell type, cell cycle, cell activation, age of donor animal, passage number of cell line, presence of specific growth/mitogenic factors, temperature, shape, and cell density/packing during exposures have been shown to play a role in mediating ELF interactions with cells. Most recently, reports of single-cell studies usher in a new direction for research that can be termed microbioelectromagnetics. Single-cell digital microscopy introduces a new approach to answer the above questions with potential for real-time microdosimetry and bioeffects limited only by the spatial resolution of state-of-the-art microscopy, which is approximately 0.1 /μm. Digital imaging microscopy should therefore permit the quantitative assessment of spatial and temporal features of ELF field interactions within living single cells.

  16. Size effects of effective Young's modulus for periodic cellular materials

    Institute of Scientific and Technical Information of China (English)

    DAI GaoMing; ZHANG WeiHong

    2009-01-01

    With the wide demands of cellular materials applications in aerospace and civil engineering, research effort sacrificed for this type of materials attains nowadays a higher level than ever before. This paper is focused on the prediction methods of effective Young's modulus for periodical cellular materials. Based on comprehensive studies of the existing homogenization method (HM), the G-A meso-me-chanice method (G-A MMM) and the stretching energy method (SEM) that are unable to reflect the size effect, we propose the bending energy method (BEM) for the first time, and a comparative study of these four methods is further made to show the generality and the capability of capturing the size effect of the BEM method. Meanwhile, the underlying characteristics of each method and their relations are clarified. To do this, the detailed finite element computing and existing experimental results of hex-agonal honeycombs from the literature are adopted as the standard of comparison for the above four methods. Stretch and bending models of periodical cellular materials are taken into account, respec-tively for the comparison of stretch and flexural displacements resulting from the above methods. We conclude that the BEM has the strong ability of both predicting the effective Young's modulus and re- vealing the size effect. Such a method is also able to predict well the variations of structural displace-ments in terms of the cell size under stretching and bending loads including the non-monotonous variations for the hexagonal cell. On the contrary, other three methods can only predict the limited re- sults whenever the cell size tends to be infinitely small.

  17. Cellular Dynamic Simulator: An Event Driven Molecular Simulation Environment for Cellular Physiology

    OpenAIRE

    Byrne, Michael J.; Waxham, M. Neal; Kubota, Yoshihisa

    2010-01-01

    In this paper, we present the Cellular Dynamic Simulator (CDS) for simulating diffusion and chemical reactions within crowded molecular environments. CDS is based on a novel event driven algorithm specifically designed for precise calculation of the timing of collisions, reactions and other events for each individual molecule in the environment. Generic mesh based compartments allow the creation / importation of very simple or detailed cellular structures that exist in a 3D environment. Multi...

  18. Mobile Node Localization in Cellular Networks

    Directory of Open Access Journals (Sweden)

    Yasir Malik

    2012-01-01

    Full Text Available Location information is the major component in location based applications. This information is used in different safety and service oriented applications to provide users with services according to their Geolocation. There are many approaches to locate mobile nodes in indoor and outdoor environments. In thispaper, we are interested in outdoor localization particularly in cellular networks of mobile nodes andpresented a localization method based on cell and user location information. Our localization method is based on hello message delay (sending and receiving time and coordinate information of Base Transceiver Station (BTSs. To validate our method across cellular network, we implemented and simulated our method in two scenarios i.e. maintaining database of base stations in centralize and distributed system. Simulation results show the effectiveness of our approach and its implementation applicability in telecommunication systems.

  19. External insulation with cellular plastic materials

    DEFF Research Database (Denmark)

    Sørensen, Lars Schiøtt; Nielsen, Anker

    2014-01-01

    External thermal insulation composite systems (ETICS) can be used as extra insulation of existing buildings. The system can be made of cellular plastic materials or mineral wool. There is a European Technical guideline, ETAG 004, that describe the tests that shall be conducted on such systems....... This paper gives a comparison of systems with mineral wool and cellular plastic, based on experience from practice and literature. It is important to look at the details in the system and at long time stability of the properties such as thermal insulation, moisture and fire. Investigation of fire properties...... must be done before utilisation of the system, including the risk of fire spread from one storey to the next for practical solutions. An elaboration of fire spread risks require thermo physic knowledge about ignition temperatures, critical radiation, upward flame spread velocities etc. of the actual...

  20. Designing beauty the art of cellular automata

    CERN Document Server

    Martínez, Genaro

    2016-01-01

    This fascinating, colourful book offers in-depth insights and first-hand working experiences in the production of art works, using simple computational models with rich morphological behaviour, at the edge of mathematics, computer science, physics and biology. It organically combines ground breaking scientific discoveries in the theory of computation and complex systems with artistic representations of the research results. In this appealing book mathematicians, computer scientists, physicists, and engineers brought together marvelous and esoteric patterns generated by cellular automata, which are arrays of simple machines with complex behavior. Configurations produced by cellular automata uncover mechanics of dynamic patterns formation, their propagation and interaction in natural systems: heart pacemaker, bacterial membrane proteins, chemical rectors, water permeation in soil, compressed gas, cell division, population dynamics, reaction-diffusion media and self-organisation. The book inspires artists to tak...

  1. Astrobiological Complexity with Probabilistic Cellular Automata

    CERN Document Server

    Vukotić, B

    2012-01-01

    Search for extraterrestrial life and intelligence constitutes one of the major endeavors in science, but has yet been quantitatively modeled only rarely and in a cursory and superficial fashion. We argue that probabilistic cellular automata (PCA) represent the best quantitative framework for modeling astrobiological history of the Milky Way and its Galactic Habitable Zone. The relevant astrobiological parameters are to be modeled as the elements of the input probability matrix for the PCA kernel. With the underlying simplicity of the cellular automata constructs, this approach enables a quick analysis of large and ambiguous input parameters' space. We perform a simple clustering analysis of typical astrobiological histories and discuss the relevant boundary conditions of practical importance for planning and guiding actual empirical astrobiological and SETI projects. In addition to showing how the present framework is adaptable to more complex situations and updated observational databases from current and ne...

  2. Molecular kinesis in cellular function and plasticity.

    Science.gov (United States)

    Tiedge, H; Bloom, F E; Richter, D

    2001-06-19

    Intracellular transport and localization of cellular components are essential for the functional organization and plasticity of eukaryotic cells. Although the elucidation of protein transport mechanisms has made impressive progress in recent years, intracellular transport of RNA remains less well understood. The National Academy of Sciences Colloquium on Molecular Kinesis in Cellular Function and Plasticity therefore was devised as an interdisciplinary platform for participants to discuss intracellular molecular transport from a variety of different perspectives. Topics covered at the meeting included RNA metabolism and transport, mechanisms of protein synthesis and localization, the formation of complex interactive protein ensembles, and the relevance of such mechanisms for activity-dependent regulation and synaptic plasticity in neurons. It was the overall objective of the colloquium to generate momentum and cohesion for the emerging research field of molecular kinesis.

  3. Cellular and Molecular Mechanisms of AKI.

    Science.gov (United States)

    Agarwal, Anupam; Dong, Zheng; Harris, Raymond; Murray, Patrick; Parikh, Samir M; Rosner, Mitchell H; Kellum, John A; Ronco, Claudio

    2016-05-01

    In this article, we review the current evidence for the cellular and molecular mechanisms of AKI, focusing on epithelial cell pathobiology and related cell-cell interactions, using ischemic AKI as a model. Highlighted are the clinical relevance of cellular and molecular targets that have been investigated in experimental models of ischemic AKI and how such models might be improved to optimize translation into successful clinical trials. In particular, development of more context-specific animal models with greater relevance to human AKI is urgently needed. Comorbidities that could alter patient susceptibility to AKI, such as underlying diabetes, aging, obesity, cancer, and CKD, should also be considered in developing these models. Finally, harmonization between academia and industry for more clinically relevant preclinical testing of potential therapeutic targets and better translational clinical trial design is also needed to achieve the goal of developing effective interventions for AKI. PMID:26860342

  4. Cellular and molecular introduction to brain development.

    Science.gov (United States)

    Jiang, Xiangning; Nardelli, Jeannette

    2016-08-01

    Advances in the study of brain development over the last decades, especially recent findings regarding the evolutionary expansion of the human neocortex, and large-scale analyses of the proteome/transcriptome in the human brain, have offered novel insights into the molecular mechanisms guiding neural maturation, and the pathophysiology of multiple forms of neurological disorders. As a preamble to reviews of this issue, we provide an overview of the cellular, molecular and genetic bases of brain development with an emphasis on the major mechanisms associated with landmarks of normal neural development in the embryonic stage and early postnatal life, including neural stem/progenitor cell proliferation, cortical neuronal migration, evolution and folding of the cerebral cortex, synaptogenesis and neural circuit development, gliogenesis and myelination. We will only briefly depict developmental disorders that result from perturbations of these cellular or molecular mechanisms, and the most common perinatal brain injuries that could disturb normal brain development. PMID:26184894

  5. Cellularity of certain quantum endomorphism algebras

    DEFF Research Database (Denmark)

    Andersen, Henning Haahr; Lehrer, G. I.; Zhang, R.

    Let $\\tA=\\Z[q^{\\pm \\frac{1}{2}}][([d]!)\\inv]$ and let $\\Delta_{\\tA}(d)$ be an integral form of the Weyl module of highest weight $d \\in \\N$ of the quantised enveloping algebra $\\U_{\\tA}$ of $\\fsl_2$. We exhibit for all positive integers $r$ an explicit cellular structure for $\\End_{\\U_{\\tA}}(\\Del......Let $\\tA=\\Z[q^{\\pm \\frac{1}{2}}][([d]!)\\inv]$ and let $\\Delta_{\\tA}(d)$ be an integral form of the Weyl module of highest weight $d \\in \\N$ of the quantised enveloping algebra $\\U_{\\tA}$ of $\\fsl_2$. We exhibit for all positive integers $r$ an explicit cellular structure for $\\End...

  6. Cellular automata in image processing and geometry

    CERN Document Server

    Adamatzky, Andrew; Sun, Xianfang

    2014-01-01

    The book presents findings, views and ideas on what exact problems of image processing, pattern recognition and generation can be efficiently solved by cellular automata architectures. This volume provides a convenient collection in this area, in which publications are otherwise widely scattered throughout the literature. The topics covered include image compression and resizing; skeletonization, erosion and dilation; convex hull computation, edge detection and segmentation; forgery detection and content based retrieval; and pattern generation. The book advances the theory of image processing, pattern recognition and generation as well as the design of efficient algorithms and hardware for parallel image processing and analysis. It is aimed at computer scientists, software programmers, electronic engineers, mathematicians and physicists, and at everyone who studies or develops cellular automaton algorithms and tools for image processing and analysis, or develops novel architectures and implementations of mass...

  7. Cellular and molecular basis of cerebellar development

    Science.gov (United States)

    Martinez, Salvador; Andreu, Abraham; Mecklenburg, Nora; Echevarria, Diego

    2013-01-01

    Historically, the molecular and cellular mechanisms of cerebellar development were investigated through structural descriptions and studying spontaneous mutations in animal models and humans. Advances in experimental embryology, genetic engineering, and neuroimaging techniques render today the possibility to approach the analysis of molecular mechanisms underlying histogenesis and morphogenesis of the cerebellum by experimental designs. Several genes and molecules were identified to be involved in the cerebellar plate regionalization, specification, and differentiation of cerebellar neurons, as well as the establishment of cellular migratory routes and the subsequent neuronal connectivity. Indeed, pattern formation of the cerebellum requires the adequate orchestration of both key morphogenetic signals, arising from distinct brain regions, and local expression of specific transcription factors. Thus, the present review wants to revisit and discuss these morphogenetic and molecular mechanisms taking place during cerebellar development in order to understand causal processes regulating cerebellar cytoarchitecture, its highly topographically ordered circuitry and its role in brain function. PMID:23805080

  8. Cellular and Molecular Basis of Cerebellar Development

    Directory of Open Access Journals (Sweden)

    Salvador eMartinez

    2013-06-01

    Full Text Available Historically, the molecular and cellular mechanisms of cerebellar development were investigated through structural descriptions and studying spontaneous mutations in animal models and humans. Advances in experimental embryology, genetic engineering and neuroimaging techniques render today the possibility to approach the analysis of molecular mechanisms underlying histogenesis and morphogenesis of the cerebellum by experimental designs. Several genes and molecules were identified to be involved in the cerebellar plate regionalization, specification and differentiation of cerebellar neurons, as well as the establishment of cellular migratory routes and the subsequent neuronal connectivity. Indeed, pattern formation of the cerebellum requires the adequate orchestration of both key morphogenetic signals, arising from distinct brain regions, and local expression of specific transcription factors. Thus, the present review wants to revisit and discuss these morphogenetic and molecular mechanisms taking place during cerebellar development in order to understand causal processes regulating cerebellar cytoarchitecture, its highly topographically ordered circuitry and its role in brain function.

  9. WD40 proteins propel cellular networks.

    Science.gov (United States)

    Stirnimann, Christian U; Petsalaki, Evangelia; Russell, Robert B; Müller, Christoph W

    2010-10-01

    Recent findings indicate that WD40 domains play central roles in biological processes by acting as hubs in cellular networks; however, they have been studied less intensely than other common domains, such as the kinase, PDZ or SH3 domains. As suggested by various interactome studies, they are among the most promiscuous interactors. Structural studies suggest that this property stems from their ability, as scaffolds, to interact with diverse proteins, peptides or nucleic acids using multiple surfaces or modes of interaction. A general scaffolding role is supported by the fact that no WD40 domain has been found with intrinsic enzymatic activity despite often being part of large molecular machines. We discuss the WD40 domain distributions in protein networks and structures of WD40-containing assemblies to demonstrate their versatility in mediating critical cellular functions.

  10. Quantum features of natural cellular automata

    CERN Document Server

    Elze, Hans-Thomas

    2016-01-01

    Cellular automata can show well known features of quantum mechanics, such as a linear rule according to which they evolve and which resembles a discretized version of the Schroedinger equation. This includes corresponding conservation laws. The class of "natural" Hamiltonian cellular automata is based exclusively on integer-valued variables and couplings and their dynamics derives from an Action Principle. They can be mapped reversibly to continuum models by applying Sampling Theory. Thus, "deformed" quantum mechanical models with a finite discreteness scale $l$ are obtained, which for $l\\rightarrow 0$ reproduce familiar continuum results. We have recently demonstrated that such automata can form "multipartite" systems consistently with the tensor product structures of nonrelativistic many-body quantum mechanics, while interacting and maintaining the linear evolution. Consequently, the Superposition Principle fully applies for such primitive discrete deterministic automata and their composites and can produce...

  11. A cellular glass substrate solar concentrator

    Science.gov (United States)

    Bedard, R.; Bell, D.

    1980-01-01

    The design of a second generation point focusing solar concentration is discussed. The design is based on reflective gores fabricated of thin glass mirror bonded continuously to a contoured substrate of cellular glass. The concentrator aperture and structural stiffness was optimized for minimum concentrator cost given the performance requirement of delivering 56 kWth to a 22 cm diameter receiver aperture with a direct normal insolation of 845 watts sq m and an operating wind of 50 kmph. The reflective panel, support structure, drives, foundation and instrumentation and control subsystem designs, optimized for minimum cost, are summarized. The use of cellular glass as a reflective panel substrate material is shown to offer significant weight and cost advantages compared to existing technology materials.

  12. SELF-ORGANIZED CRITICALITY AND CELLULAR AUTOMATA

    Energy Technology Data Exchange (ETDEWEB)

    CREUTZ,M.

    2007-01-01

    Cellular automata provide a fascinating class of dynamical systems based on very simple rules of evolution yet capable of displaying highly complex behavior. These include simplified models for many phenomena seen in nature. Among other things, they provide insight into self-organized criticality, wherein dissipative systems naturally drive themselves to a critical state with important phenomena occurring over a wide range of length and the scales. This article begins with an overview of self-organized criticality. This is followed by a discussion of a few examples of simple cellular automaton systems, some of which may exhibit critical behavior. Finally, some of the fascinating exact mathematical properties of the Bak-Tang-Wiesenfeld sand-pile model [1] are discussed.

  13. A Modified Sensitive Driving Cellular Automaton Model

    Institute of Scientific and Technical Information of China (English)

    GE Hong-Xia; DAI Shi-Qiang; DONG Li-Yun; LEI Li

    2005-01-01

    A modified cellular automaton model for traffic flow on highway is proposed with a novel concept about the variable security gap. The concept is first introduced into the original Nagel-Schreckenberg model, which is called the non-sensitive driving cellular automaton model. And then it is incorporated with a sensitive driving NaSch model,in which the randomization brake is arranged before the deterministic deceleration. A parameter related to the variable security gap is determined through simulation. Comparison of the simulation results indicates that the variable security gap has different influence on the two models. The fundamental diagram obtained by simulation with the modified sensitive driving NaSch model shows that the maximumflow are in good agreement with the observed data, indicating that the presented model is more reasonable and realistic.

  14. Cellular development of the human cochlea and the regenerative potential of hair follicle bulge stem cells

    NARCIS (Netherlands)

    Locher, heiko

    2015-01-01

    The embryonic development of the human cochlea (the organ of hearing) has been investigated for over one hundred years. However, little is still known about the development on a cellular and protein level, which is important to better understand etiologies and pathologies of various types of sensori

  15. Tracing Dynamics and Clonal Heterogeneity of Cbx7-Induced Leukemic Stem Cells by Cellular Barcoding

    NARCIS (Netherlands)

    Klauke, Karin; Broekhuis, Mathilde J. C.; Weersing, Ellen; Dethmers-Ausema, Albertina; Ritsema, Martha; Gonzalez, Marta Vila; Zwart, Erik; Bystrykh, Leonid V.; de Haan, Gerald

    2015-01-01

    Accurate monitoring of tumor dynamics and leukemic stem cell (LSC) heterogeneity is important for the development of personalized cancer therapies. In this study, we experimentally induced distinct types of leukemia in mice by enforced expression of Cbx7. Simultaneous cellular barcoding allowed for

  16. Design of a modular product architecture and a cellular manufacturing layout : A concurrent engineering approach

    NARCIS (Netherlands)

    Slomp, J; McGinnis, LF; Ahmad, MM; Sullivan, WG

    1996-01-01

    This paper formalizes the problem of a concurrent design of a modular product architecture and a cellular manufacturing layout and presents a way to deal with this design problem. First, the different types of a modular product architecture are explained as well as important implications of modulari

  17. Cellular and molecular basis of mammary microcalcifications

    OpenAIRE

    Cox, Rachel

    2011-01-01

    Mammary microcalcifications represent one of the most reliable mammographic features of non-palpable breast cancer and are often the sole indicator of the disease. However, it is unknown whether these microcalcifications are a sign of degeneration or an active cellular process. The aims of this project were to establish and characterise an in vitro model of mammary mineralisation in monolayer, 3D scaffolds and in vivo and to investigate the molecular mechanisms involved in this process, focus...

  18. Exponential Stability for Delayed Cellular Neural Networks

    Institute of Scientific and Technical Information of China (English)

    YANG Jin-xiang; ZHONG Shou-ming; YAN Ke-yu

    2005-01-01

    The exponential stability of the delayed cellular neural networks (DCNN's) is investigated. By dividing the network state variables into some parts according to the characters of the neural networks, some new sufficient conditions of exponential stability are derived via constructing a Liapunov function. It is shown that the conditions differ from previous ones. The new conditions, which are associated with some initial value, are represented by some blocks of the interconnection matrix.

  19. Light weight cellular structures based on aluminium

    Energy Technology Data Exchange (ETDEWEB)

    Prakash, O. [Indian Inst. of Tech., Kanpur (India); Embury, J.D.; Sinclair, C. [McMaster Univ., Hamilton, ON (Canada); Sang, H. [Queen`s Univ., Kingston, ON (Canada); Silvetti, P. [Cordoba Univ. Nacional (Argentina). Facultad de Ciencias Exactas, Fisicas y Naturales

    1997-02-01

    An interesting form of lightweight material which has emerged in the past 2 decades is metallic foam. This paper deals with the basic concepts of making metallic foams and a detailed study of foams produced from Al-SiC. In addition, some aspects of cellular solids based on honeycomb structures are outlined including the concept of producing both two-phase foams and foams with composite walls.

  20. Animal and cellular models of human disease

    OpenAIRE

    Arends, Mark; White, Eric; Whitelaw, Christopher

    2016-01-01

    In this eighteenth (2016) Annual Review Issue of The Journal of Pathology, we present a collection of 19 invited review articles that cover different aspects of cellular and animal models of disease. These include genetically-engineered models, chemically-induced models, naturally-occurring models, and combinations thereof, with the focus on recent methodological and conceptual developments across a wide range of human diseases.

  1. Cellular immune findings in Lyme disease.

    Science.gov (United States)

    Sigal, L. H.; Moffat, C. M.; Steere, A. C.; Dwyer, J. M.

    1984-01-01

    From 1981 through 1983, we did the first testing of cellular immunity in Lyme disease. Active established Lyme disease was often associated with lymphopenia, less spontaneous suppressor cell activity than normal, and a heightened response of lymphocytes to phytohemagglutinin and Lyme spirochetal antigens. Thus, a major feature of the immune response during active disease seems to be a lessening of suppression, but it is not yet known whether this response plays a role in the pathophysiology of the disease. PMID:6240164

  2. Spectrum sharing for future mobile cellular systems

    OpenAIRE

    Bennis, M

    2009-01-01

    Abstract Spectrum sharing has become a high priority research area over the past few years. The motivation behind this lies in the fact that the limited spectrum is currently inefficiently utilized. As recognized by the World radio communication conference (WRC)-07, the amount of identified spectrum is not large enough to support large bandwidths for a substantial number of operators. Therefore, it is paramount for future mobile cellular systems to share the frequency spectrum and coexist ...

  3. Cellular factors required for papillomavirus DNA replication.

    OpenAIRE

    Melendy, T; Sedman, J; Stenlund, A

    1995-01-01

    In vitro replication of papillomavirus DNA has been carried out with a combination of purified proteins and partially purified extracts made from human cells. DNA synthesis requires the viral E1 protein and the papillomavirus origin of replication. The E2 protein stimulates DNA synthesis in a binding site-independent manner. Papillomavirus DNA replication is also dependent on the cellular factors replication protein A, replication factor C, and proliferating-cell nuclear antigen as well as a ...

  4. Sumo and the cellular stress response

    OpenAIRE

    Enserink, Jorrit M.

    2015-01-01

    The ubiquitin family member Sumo has important functions in many cellular processes including DNA repair, transcription and cell division. Numerous studies have shown that Sumo is essential for maintaining cell homeostasis when the cell encounters endogenous or environmental stress, such as osmotic stress, hypoxia, heat shock, genotoxic stress, and nutrient stress. Regulation of transcription is a key component of the Sumo stress response, and multiple mechanisms have been described by which ...

  5. The flow of forces through cellular materials

    OpenAIRE

    Berger, Mitchell A.

    2012-01-01

    Describing and measuring the elastic properties of cellular materials such as honeycombs and foams can be a difficult problem when the cell structure is disordered. This paper suggests that tracking the flow of forces through the material can help in visualizing and understanding how the geometry of the cell structure affects the elastic behaviour. The mean strain tensor for a sample of material can be calculated by summing over the force paths, weighted by the strengths of the paths. This me...

  6. Imaging cellular and molecular biological functions

    Energy Technology Data Exchange (ETDEWEB)

    Shorte, S.L. [Institut Pasteur, 75 - Paris (France). Plateforme d' Imagerie Dynamique PFID-Imagopole; Frischknecht, F. (eds.) [Heidelberg Univ. Medical School (Germany). Dept. of Parasitology

    2007-07-01

    'Imaging cellular and molecular biological function' provides a unique selection of essays by leading experts, aiming at scientist and student alike who are interested in all aspects of modern imaging, from its application and up-scaling to its development. Indeed the philosophy of this volume is to provide student, researcher, PI, professional or provost the means to enter this applications field with confidence, and to construct the means to answer their own specific questions. (orig.)

  7. Cellular and synaptic network defects in autism

    OpenAIRE

    Peça, João; Feng, Guoping

    2012-01-01

    Many candidate genes are now thought to confer susceptibility to autism spectrum disorders (ASDs). Here we review four interrelated complexes, each composed of multiple families of genes that functionally coalesce on common cellular pathways. We illustrate a common thread in the organization of glutamatergic synapses and suggest a link between genes involved in Tuberous Sclerosis Complex, Fragile X syndrome, Angelman syndrome and several synaptic ASD candidate genes. When viewed in this conte...

  8. Cellular events and biomarkers of wound healing

    OpenAIRE

    Shah Jumaat Mohd Yussof; Effat Omar; Pai, Dinker R.; Suneet Sood

    2012-01-01

    Researchers have identified several of the cellular events associated with wound healing. Platelets, neutrophils, macrophages, and fibroblasts primarily contribute to the process. They release cytokines including interleukins (ILs) and TNF-α, and growth factors, of which platelet-derived growth factor (PDGF) is perhaps the most important. The cytokines and growth factors manipulate the inflammatory phase of healing. Cytokines are chemotactic for white cells and fibroblasts, while the growth f...

  9. Oxidative stress action in cellular aging

    OpenAIRE

    Monique Cristine de Oliveira; João Paulo Ferreira Schoffen

    2010-01-01

    Various theories try to explain the biological aging by changing the functions and structure of organic systems and cells. During lifetime, free radicals in the oxidative stress lead to lipid peroxidation of cellular membranes, homeostasis imbalance, chemical residues formation, gene mutations in DNA, dysfunction of certain organelles, and the arise of diseases due to cell death and/or injury. This review describes the action of oxidative stress in the cells aging process, emphasizing the fac...

  10. Cellular immune findings in Lyme disease.

    OpenAIRE

    Sigal, L. H.; Moffat, C. M.; Steere, A. C.; Dwyer, J. M.

    1984-01-01

    From 1981 through 1983, we did the first testing of cellular immunity in Lyme disease. Active established Lyme disease was often associated with lymphopenia, less spontaneous suppressor cell activity than normal, and a heightened response of lymphocytes to phytohemagglutinin and Lyme spirochetal antigens. Thus, a major feature of the immune response during active disease seems to be a lessening of suppression, but it is not yet known whether this response plays a role in the pathophysiology o...

  11. Introduction to Tissular and Cellular Engineering

    Institute of Scientific and Technical Information of China (English)

    JF; STOLTZ

    2005-01-01

    Most human tissues do not regenerate spontaneously, which is why cellular therapies and tissular engineering are promising alternatives. The principle is simple: cells are sampled in a patient and introduced in the damaged tissue or in a tridimentional porous support and cultivated in a bioreactor in which the physico-chemical and mechanical parameters are controlled. Once the tissues (or the cells) are mature they may be implanted. In parallel, the development of biotherapies with stem cells is a field of ...

  12. Empirical multiscale networks of cellular regulation.

    Directory of Open Access Journals (Sweden)

    Benjamin de Bivort

    2007-10-01

    Full Text Available Grouping genes by similarity of expression across multiple cellular conditions enables the identification of cellular modules. The known functions of genes enable the characterization of the aggregate biological functions of these modules. In this paper, we use a high-throughput approach to identify the effective mutual regulatory interactions between modules composed of mouse genes from the Alliance for Cell Signaling (AfCS murine B-lymphocyte database which tracks the response of approximately 15,000 genes following chemokine perturbation. This analysis reveals principles of cellular organization that we discuss along four conceptual axes. (1 Regulatory implications: the derived collection of influences between any two modules quantifies intuitive as well as unexpected regulatory interactions. (2 Behavior across scales: trends across global networks of varying resolution (composed of various numbers of modules reveal principles of assembly of high-level behaviors from smaller components. (3 Temporal behavior: tracking the mutual module influences over different time intervals provides features of regulation dynamics such as duration, persistence, and periodicity. (4 Gene Ontology correspondence: the association of modules to known biological roles of individual genes describes the organization of functions within coexpressed modules of various sizes. We present key specific results in each of these four areas, as well as derive general principles of cellular organization. At the coarsest scale, the entire transcriptional network contains five divisions: two divisions devoted to ATP production/biosynthesis and DNA replication that activate all other divisions, an "extracellular interaction" division that represses all other divisions, and two divisions (proliferation/differentiation and membrane infrastructure that activate and repress other divisions in specific ways consistent with cell cycle control.

  13. Cellular responses to environmental DNA damage

    Energy Technology Data Exchange (ETDEWEB)

    1994-08-01

    This volume contains the proceedings of the conference entitled Cellular Responses to Environmental DNA Damage held in Banff,Alberta December 1--6, 1991. The conference addresses various aspects of DNA repair in sessions titled DNA repair; Basic Mechanisms; Lesions; Systems; Inducible Responses; Mutagenesis; Human Population Response Heterogeneity; Intragenomic DNA Repair Heterogeneity; DNA Repair Gene Cloning; Aging; Human Genetic Disease; and Carcinogenesis. Individual papers are represented as abstracts of about one page in length.

  14. Stability of Stochastic Neutral Cellular Neural Networks

    Science.gov (United States)

    Chen, Ling; Zhao, Hongyong

    In this paper, we study a class of stochastic neutral cellular neural networks. By constructing a suitable Lyapunov functional and employing the nonnegative semi-martingale convergence theorem we give some sufficient conditions ensuring the almost sure exponential stability of the networks. The results obtained are helpful to design stability of networks when stochastic noise is taken into consideration. Finally, two examples are provided to show the correctness of our analysis.

  15. Cellular Signaling in the Bovine Antral Follicles

    OpenAIRE

    Juan F. Vásquez - Cano; Martha Olivera - A.

    2010-01-01

    Antral follicle development in the ovary of female cattle is the product of a complex of endocrine, paracrine and autocrine relationships. The interactions of the pituitary gonadotropins over granulosa and theca cells prepare the follicle to produce estradiol and for the final stages of maturation of the oocyte and its potencial ovulation or atresia inside subordinate follicles. It is a dynamic event where cellular signaling patterns changes sequentiallyand quickly at different stages of foll...

  16. Cellular Automation of Galactic Habitable Zone

    CERN Document Server

    Vukotic, Branislav

    2010-01-01

    We present a preliminary results of our Galactic Habitable Zone (GHZ) 2D probabilistic cellular automata models. The relevant time-scales (emergence of life, it's diversification and evolution influenced with the global risk function) are modeled as the probability matrix elements and are chosen in accordance with the Copernican principle to be well-represented by the data inferred from the Earth's fossil record. With Fermi's paradox as a main boundary condition the resulting histories of astrobiological landscape are discussed.

  17. Cognitive resource management for heterogeneous cellular networks

    CERN Document Server

    Liu, Yongkang

    2014-01-01

    This Springer Brief focuses on cognitive resource management in heterogeneous cellular networks (Het Net) with small cell deployment for the LTE-Advanced system. It introduces the Het Net features, presents practical approaches using cognitive radio technology in accommodating small cell data relay and optimizing resource allocation and examines the effectiveness of resource management among small cells given limited coordination bandwidth and wireless channel uncertainty. The authors introduce different network characteristics of small cell, investigate the mesh of small cell access points in

  18. Important cellular targets for antimicrobial photodynamic therapy.

    Science.gov (United States)

    Awad, Mariam M; Tovmasyan, Artak; Craik, James D; Batinic-Haberle, Ines; Benov, Ludmil T

    2016-09-01

    The persistent problem of antibiotic resistance has created a strong demand for new methods for therapy and disinfection. Photodynamic inactivation (PDI) of microbes has demonstrated promising results for eradication of antibiotic-resistant strains. PDI is based on the use of a photosensitive compound (photosensitizer, PS), which upon illumination with visible light generates reactive species capable of damaging and killing microorganisms. Since photogenerated reactive species are short lived, damage is limited to close proximity of the PS. It is reasonable to expect that the larger the number of damaged targets is and the greater their variety is, the higher the efficiency of PDI is and the lower the chances for development of resistance are. Exact molecular mechanisms and specific targets whose damage is essential for microbial inactivation have not been unequivocally established. Two main cellular components, DNA and plasma membrane, are regarded as the most important PDI targets. Using Zn porphyrin-based PSs and Escherichia coli as a model Gram-negative microorganism, we demonstrate that efficient photoinactivation of bacteria can be achieved without detectable DNA modification. Among the cellular components which are modified early during illumination and constitute key PDI targets are cytosolic enzymes, membrane-bound protein complexes, and the plasma membrane. As a result, membrane barrier function is lost, and energy and reducing equivalent production is disrupted, which in turn compromises cell defense mechanisms, thus augmenting the photoinduced oxidative injury. In conclusion, high PDI antimicrobial effectiveness does not necessarily require impairment of a specific critical cellular component and can be achieved by inducing damage to multiple cellular targets. PMID:27221289

  19. Cellular Kinetics of Perivascular MSC Precursors

    Directory of Open Access Journals (Sweden)

    William C. W. Chen

    2013-01-01

    Full Text Available Mesenchymal stem/stromal cells (MSCs and MSC-like multipotent stem/progenitor cells have been widely investigated for regenerative medicine and deemed promising in clinical applications. In order to further improve MSC-based stem cell therapeutics, it is important to understand the cellular kinetics and functional roles of MSCs in the dynamic regenerative processes. However, due to the heterogeneous nature of typical MSC cultures, their native identity and anatomical localization in the body have remained unclear, making it difficult to decipher the existence of distinct cell subsets within the MSC entity. Recent studies have shown that several blood-vessel-derived precursor cell populations, purified by flow cytometry from multiple human organs, give rise to bona fide MSCs, suggesting that the vasculature serves as a systemic reservoir of MSC-like stem/progenitor cells. Using individually purified MSC-like precursor cell subsets, we and other researchers have been able to investigate the differential phenotypes and regenerative capacities of these contributing cellular constituents in the MSC pool. In this review, we will discuss the identification and characterization of perivascular MSC precursors, including pericytes and adventitial cells, and focus on their cellular kinetics: cell adhesion, migration, engraftment, homing, and intercellular cross-talk during tissue repair and regeneration.

  20. HDACi: cellular effects, opportunities for restorative dentistry.

    LENUS (Irish Health Repository)

    Duncan, H F

    2011-12-01

    Acetylation of histone and non-histone proteins alters gene expression and induces a host of cellular effects. The acetylation process is homeostatically balanced by two groups of cellular enzymes, histone acetyltransferases (HATs) and histone deacetylases (HDACs). HAT activity relaxes the structure of the human chromatin, rendering it transcriptionally active, thereby increasing gene expression. In contrast, HDAC activity leads to gene silencing. The enzymatic balance can be \\'tipped\\' by histone deacetylase inhibitors (HDACi), leading to an accumulation of acetylated proteins, which subsequently modify cellular processes including stem cell differentiation, cell cycle, apoptosis, gene expression, and angiogenesis. There is a variety of natural and synthetic HDACi available, and their pleiotropic effects have contributed to diverse clinical applications, not only in cancer but also in non-cancer areas, such as chronic inflammatory disease, bone engineering, and neurodegenerative disease. Indeed, it appears that HDACi-modulated effects may differ between \\'normal\\' and transformed cells, particularly with regard to reactive oxygen species accumulation, apoptosis, proliferation, and cell cycle arrest. The potential beneficial effects of HDACi for health, resulting from their ability to regulate global gene expression by epigenetic modification of DNA-associated proteins, also offer potential for application within restorative dentistry, where they may promote dental tissue regeneration following pulpal damage.

  1. Dynamic Channel Allocation in Sectored Cellular Systems

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    It is known that dynamic channel assignment(DCA) strategy outperforms the fixed channel assignment(FCA) strategy in omni-directional antenna cellular systems. One of the most important methods used in DCA was channel borrowing. But with the emergence of cell sectorization and spatial division multiple access(SDMA) which are used to increase the capacity of cellular systems, the channel assignment faces a series of new problems. In this paper, a dynamic channel allocation scheme based on sectored cellular systems is proposed. By introducing intra-cell channel borrowing (borrowing channels from neighboring sectors) and inter-cell channel borrowing (borrowing channels from neighboring cells) methods, previous DCA strategies, including compact pattern based channel borrowing(CPCB) and greedy based dynamic channel assignment(GDCA) schemes proposed by the author, are improved significantly. The computer simulation shows that either intra-cell borrowing scheme or inter-cell borrowing scheme is efficient enough to uniform and non-uniform traffic service distributions.

  2. Influence of electric field on cellular migration

    Science.gov (United States)

    Guido, Isabella; Bodenschatz, Eberhard

    Cells have the ability to detect continuous current electric fields (EFs) and respond to them with a directed migratory movement. Dictyostelium discoideum (D.d.) cells, a key model organism for the study of eukaryotic chemotaxis, orient and migrate toward the cathode under the influence of an EF. The underlying sensing mechanism and whether it is shared by the chemotactic response pathway remains unknown. Whereas genes and proteins that mediate the electric sensing as well as that define the migration direction have been previously investigated in D.d. cells, a deeper knowledge about the cellular kinematic effects caused by the EF is still lacking. Here we show that besides triggering a directional bias the electric field influences the cellular kinematics by accelerating the movement of cells along their path. We found that the migratory velocity of the cells in an EF increases linearly with the exposure time. Through the analysis of the PI3K and Phg2 distribution in the cytosol and of the cellular adherence to the substrate we aim at elucidating whereas this speed up effect in the electric field is due to either a molecular signalling or the interaction with the substrate. This work is part of the MaxSynBio Consortium which is jointly funded by the Federal Ministry of Education and Research of Germany and the Max Planck Society.

  3. Cellular vs. organ approaches to dose estimates

    International Nuclear Information System (INIS)

    The cellular distribution of tissue-incorporated radionuclides has generally been neglected in the dosimetry of internal emitters. Traditional dosimetry assumes homogeneous distribution of radionuclides in organs of interest, while presuming that the ranges of particulate radiations are large relative to typical cell diameters. The macroscopic distribution of dose thus calculated has generally served as a sufficient approximation for the energy deposited within radiosensitive sites. However, with the increasing utilization of intracellular agents, such as thallium-201, it has become necessary to examine the microscopic distribution of energy at the cellular level. This is particularly important in the instance of radionuclides that decay by electron capture or by internal conversion with the release of Auger and Coster-Kronig electrons. In many instances, these electrons are released as a dense shower of low-energy particles with ranges of subcellular dimensions. The high electron density in the immediate vicinity of the decaying atom produces a focal deposition of energy that far exceeds the average dose taken over several cell diameters. These studies point out the increasing need to take into account the microscopic distribution of dose on the cellular level as radionuclides distributed in cells become more commonplace, especially if the decay involves electron capture or internal conversion. As radiotracers are developed for the measurement of intracellular functions these factors should be given greater consideration. 16 references, 5 figures, 5 tables

  4. Coordination of autophagy with other cellular activities

    Institute of Scientific and Technical Information of China (English)

    Yan WANG; Zheng-hong QIN

    2013-01-01

    The cell biological phenomenon of autophagy has attracted increasing attention in recent years,partly as a consequence of the discovery of key components of its cellular machinery.Autophagy plays a crucial role in a myriad of cellular functions.Autophagy has its own regulatory mechanisms,but this process is not isolated.Autophagy is coordinated with other cellular activities to maintain cell homeostasis.Autophagy is critical for a range of human physiological processes.The multifunctional roles of autophagy are explained by its ability to interact with several key components of various cell pathways.In this review,we focus on the coordination between autophagy and other physiological processes,including the ubiquitin-proteasome system (UPS),energy homeostasis,aging,programmed cell death,the immune responses,microbial invasion and inflammation.The insights gained from investigating autophagic networks should increase our understanding of their roles in human diseases and their potential as targets for therapeutic intervention.

  5. Electrostatic bio-manipulation for the modification of cellular functions

    Science.gov (United States)

    Washizu, Masao

    2013-03-01

    The use of electrostatic field effects, including field-induced reversible-breakdown of the membrane and dielectrophoresis (DEP), in microfabricated structures are investigated. With the use of field constriction created by a micro-orifice whose diameter is smaller than the cells, controlled magnitude of pulsed voltage can be applied across the cell membrane regardless of the cell size, shape or orientation. As a result, the breakdown occurs reproducibly and with minimal invasiveness. The breakdown is used for two purposes, electroporation by which foreign substances can be fed into cells, and electrofusion which creates genetic and/or cytoplasmic mixture among two cells. When GFP plasmid is fed into MSC cell, the gene expression started within 2 hours, and finally observed in more than 50% of cells. For cell fusion, several ten percent fusion yield is achieved for most cell types, with the colony formation in several percents. Timing-controlled feeding foreign substances or mixing cellular contents, with high-yield and low-invasiveness, is expected to bring about a new technology for both genetic and epigenetic modifications of cellular functions, in such field as regenerative medicine.

  6. Measurements of Electromagnetic Fields Emitted from Cellular Base Stations in

    Directory of Open Access Journals (Sweden)

    K. J. Ali

    2013-05-01

    Full Text Available With increasing the usage of mobile communication devices and internet network information, the entry of private telecommunications companies in Iraq has been started since 2003. These companies began to build up cellular towers to accomplish the telecommunication works but they ignore the safety conditions imposed for the health and environment that are considered in random way. These negative health effects which may cause a health risk for life beings and environment pollution. The aim of this work is to determine the safe and unsafe ranges and discuss damage caused by radiation emitted from Asia cell base stations in Shirqat city and discuses the best ways in which can be minimize its exposure level to avoid its negative health effects. Practical measurements of power density around base stations has been accomplished by using a radiation survey meter type (Radio frequency EMF Strength Meter 480846 in two ways. The first way of measurements has been accomplished at a height of 2 meters above ground for different distances from (0-300 meters .The second way is at a distance of 150 meters for different levels from (2-15 meters above ground level. The maximum measured power density is about (3 mW/m2. Results indicate that the levels of power density are far below the RF radiation exposure of USSR safety standards levels. And that means these cellular base station don't cause negative the health effect for life being if the exposure is within the acceptable international standard levels.

  7. Structural modeling of sandwich structures with lightweight cellular cores

    Science.gov (United States)

    Liu, T.; Deng, Z. C.; Lu, T. J.

    2007-10-01

    An effective single layered finite element (FE) computational model is proposed to predict the structural behavior of lightweight sandwich panels having two dimensional (2D) prismatic or three dimensional (3D) truss cores. Three different types of cellular core topology are considered: pyramidal truss core (3D), Kagome truss core (3D) and corrugated core (2D), representing three kinds of material anisotropy: orthotropic, monoclinic and general anisotropic. A homogenization technique is developed to obtain the homogenized macroscopic stiffness properties of the cellular core. In comparison with the results obtained by using detailed FE model, the single layered computational model can give acceptable predictions for both the static and dynamic behaviors of orthotropic truss core sandwich panels. However, for non-orthotropic 3D truss cores, the predictions are not so well. For both static and dynamic behaviors of a 2D corrugated core sandwich panel, the predictions derived by the single layered computational model is generally acceptable when the size of the unit cell varies within a certain range, with the predictions for moderately strong or strong corrugated cores more accurate than those for weak cores.

  8. AdCell: Ad Allocation in Cellular Networks

    CERN Document Server

    Alaei, Saeed; Liaghat, Vahid; Pei, Dan; Saha, Barna

    2011-01-01

    With more than four billion usage of cellular phones worldwide, mobile advertising has become an attractive alternative to online advertisements. In this paper, we propose a new targeted advertising policy for Wireless Service Providers (WSPs) via SMS or MMS- namely {\\em AdCell}. In our model, a WSP charges the advertisers for showing their ads. Each advertiser has a valuation for specific types of customers in various times and locations and has a limit on the maximum available budget. Each query is in the form of time and location and is associated with one individual customer. In order to achieve a non-intrusive delivery, only a limited number of ads can be sent to each customer. Recently, new services have been introduced that offer location-based advertising over cellular network that fit in our model (e.g., ShopAlerts by AT&T) . We consider both online and offline version of the AdCell problem and develop approximation algorithms with constant competitive ratio. For the online version, we assume tha...

  9. Neural networks and cellular automata in experimental high energy physics

    International Nuclear Information System (INIS)

    Within the past few years, two novel computing techniques, cellular automata and neural networks, have shown considerable promise in the solution of problems of a very high degree of complexity, such as turbulent fluid flow, image processing, and pattern recognition. Many of the problems faced in experimental high energy physics are also of this nature. Track reconstruction in wire chambers and cluster finding in cellular calorimeters, for instance, involve pattern recognition and high combinatorial complexity since many combinations of hits or cells must be considered in order to arrive at the final tracks or clusters. Here we examine in what way connective network methods can be applied to some of the problems of experimental high physics. It is found that such problems as track and cluster finding adapt naturally to these approaches. When large scale hardwired connective networks become available, it will be possible to realize solutions to such problems in a fraction of the time required by traditional methods. For certain types of problems, faster solutions are already possible using model networks implemented on vector or other massively parallel machines. It should also be possible, using existing technology, to build simplified networks that will allow detailed reconstructed event information to be used in fast trigger decisions

  10. Cellular Functions Regulated by Phosphorylation of EGFR on Tyr845

    Directory of Open Access Journals (Sweden)

    Ken-ichi Sato

    2013-05-01

    Full Text Available The Src gene product (Src and the epidermal growth factor receptor (EGFR are prototypes of oncogene products and function primarily as a cytoplasmic non-receptor tyrosine kinase and a transmembrane receptor tyrosine kinase, respectively. The identification of Src and EGFR, and the subsequent extensive investigations of these proteins have long provided cutting edge research in cancer and other molecular and cellular biological studies. In 1995, we reported that the human epidermoid carcinoma cells, A431, contain a small fraction of Src and EGFR in which these two kinase were in physical association with each other, and that Src phosphorylates EGFR on tyrosine 845 (Y845 in the Src-EGFR complex. Y845 of EGFR is located in the activation segment of the kinase domain, where many protein kinases contain kinase-activating autophosphorylation sites (e.g., cAMP-dependent protein kinase, Src family kinases, transmembrane receptor type tyrosine kinases or trans-phosphorylation sites (e.g., cyclin-dependent protein kinase, mitogen-activated protein kinase, Akt protein kinase. A number of studies have demonstrated that Y845 phosphorylation serves an important role in cancer as well as normal cells. Here we compile the experimental facts involving Src phosphorylation of EGFR on Y845, by which cell proliferation, cell cycle control, mitochondrial regulation of cell metabolism, gamete activation and other cellular functions are regulated. We also discuss the physiological relevance, as well as structural insights of the Y845 phosphorylation.

  11. Structural modeling of sandwich structures with lightweight cellular cores

    Institute of Scientific and Technical Information of China (English)

    T. Liu; Z. C. Deng; T. J. Lu

    2007-01-01

    An effective single layered finite element (FE) computational model is proposed to predict the structural behavior of lightweight sandwich panels having two dimensional (2D) prismatic or three dimensional (3D) truss cores.Three different types of cellular core topology are considered: pyramidal truss core (3D), Kagome truss core (3D) and corrugated core (2D), representing three kinds of material anisotropy: orthotropic, monoclinic and general anisotropic. A homogenization technique is developed to obtain the homogenized macroscopic stiffness properties of the cellular core. In comparison with the results obtained by using detailed FE model, the single layered computational model cangive acceptable predictions for both the static and dynamic behaviors of orthotropic truss core sandwich panels. However, for non-orthotropic 3D truss cores, the predictions are not so well. For both static and dynamic behaviors of a 2D corrugated core sandwich panel, the predictions derived by the single layered computational model is generally acceptable when the size of the unit cell varies within a certain range, with the predictions for moderately strong or strong corrugated cores more accurate than those for weak cores.

  12. P53 family and cellular stress responses in cancer

    Directory of Open Access Journals (Sweden)

    Johanna ePflaum

    2014-10-01

    Full Text Available p53 is an important tumor suppressor gene, which is stimulated by cellular stress like ionizing radiation, hypoxia, carcinogens and oxidative stress. Upon activation p53 leads to cell cycle arrest and promotes DNA repair or induces apoptosis via several pathways. p63 and p73 are structural homologs of p53 that can act similarly to the protein but also hold functions distinct from p53. Today more than forty different isoforms of the p53 family members are known. They result from transcription via different promoters and alternative splicing. Some isoforms have carcinogenic properties and mediate resistance to chemotherapy. Therefore, expression patterns of the p53 family genes can offer prognostic information in several malignant tumors. Furthermore, the p53 family constitutes a potential target for cancer therapy. Small molecules (e.g. Nutlins, RITA, PRIMA-1, and MIRA-1 among others have been objects of intense research interest in recent years. They restore pro-apoptotic wild-type p53 function and were shown to break chemotherapeutic resistance. Due to p53 family interactions small molecules also influence p63 and p73 activity. Thus, the members of the p53 family are key players in the cellular stress response in cancer and are expected to grow in importance as therapeutic targets.

  13. Perspectivas para el control del tabaquismo en México: reflexiones sobre las políticas actuales y acciones futuras Tobacco control perspectives in Mexico: reflections on current policies and future actions

    Directory of Open Access Journals (Sweden)

    Mauricio Hernández-Ávila

    2007-01-01

    Full Text Available Según la Organización Mundial de la Salud (OMS, el tabaquismo causa en todo el mundo cerca de cinco millones de muertes al año, es decir, alrededor de 13 mil muertes diarias. En México, cerca de 60 000 defunciones por año y 165 diarias se atribuyen a esa causa, 25.6% de la población es fumador pasivo y se estima que la edad de inicio de consumo disminuyó a 12 años de edad o menos. México inició la lucha contra el tabaquismo hace dos decenios y en años recientes se combinaron acciones administrativas y legislativas relacionadas con aspectos tributarios y de regulación sanitaria. México fue el primer país en América en adherirse al Convenio Marco para el Control del Tabaco (CMCT, de la OMS, firmado por México el 12 de agosto de 2003 y ratificado el 14 de abril del año siguiente. Para el gobierno federal, la prevención y el tratamiento del tabaquismo son parte de una estrategia que busca la sinergia de los actores públicos, privados y sociales involucrados en el tema; los resultados de ese esfuerzo deberán presentarse en 2008.According to WHO, tobacco is responsible of almost five million deaths annually and around 13 thousand daily deaths in the world. Mexico has 60 thousand deaths per year and 165 per day for smoking, 25.6% of the population are passive smokers and the initiated age in tobacco consumption has diminished to 11 either 12 years or less. Two decades ago the fight against tobacco began in Mexico, and recently it combined administrative actions and legislative related to tributary matter and sanitary regulation. Mexico was the first country in America in signing the FCTC in August 12, 2003 and in April 14, 2004 ratified it, which means it is part of our legislation. At present, Federal Government prevention and tobacco treatment are focus actions integrated in an Strategy, which is looking to articulate synergy of all public, private and social actors involved in the subject, to give fulfillment to the Framework

  14. Transcriptional Activity of HTLV-I Tax Influences the Expression of Marker Genes Associated with Cellular Transformation

    Directory of Open Access Journals (Sweden)

    Francene J. Lemoine

    2001-01-01

    Full Text Available Human T cell leukemia virus type I (HTLV-I has been identified as the etiologic agent of adult T cell leukemia (ATL. HTLV-I encodes a transcriptional regulatory protein, Tax, which also functions as the viral transforming protein. Through interactions with a number of cellular transcription factors Tax can modulate cellular gene expression. Since the majority of Tax-responsive cellular genes are important regulators of cellular proliferation, the transactivating functions of Tax appear to be necessary for cellular transformation by HTLV-I. Gaining a complete understanding of the broad range of genes regulated by Tax, the temporal pattern of their expression, and their effects on cell function may identify early markers of disease progression mediated by this virus.

  15. Modeling collective & intelligent decision making of multi-cellular populations.

    Science.gov (United States)

    Shin, Yong-Jun; Mahrou, Bahareh

    2014-01-01

    In the presence of unpredictable disturbances and uncertainties, cells intelligently achieve their goals by sharing information via cell-cell communication and making collective decisions, which are more reliable compared to individual decisions. Inspired by adaptive sensor network algorithms studied in communication engineering, we propose that a multi-cellular adaptive network can convert unreliable decisions by individual cells into a more reliable cell-population decision. It is demonstrated using the effector T helper (a type of immune cell) population, which plays a critical role in initiating immune reactions in response to invading foreign agents (e.g., viruses, bacteria, etc.). While each individual cell follows a simple adaptation rule, it is the combined coordination among multiple cells that leads to the manifestation of "self-organizing" decision making via cell-cell communication.

  16. Cellular Neural Networks for NP-Hard Optimization

    Directory of Open Access Journals (Sweden)

    Mária Ercsey-Ravasz

    2009-02-01

    Full Text Available A cellular neural/nonlinear network (CNN is used for NP-hard optimization. We prove that a CNN in which the parameters of all cells can be separately controlled is the analog correspondent of a two-dimensional Ising-type (Edwards-Anderson spin-glass system. Using the properties of CNN, we show that one single operation (template always yields a local minimum of the spin-glass energy function. This way, a very fast optimization method, similar to simulated annealing, can be built. Estimating the simulation time needed on CNN-based computers, and comparing it with the time needed on normal digital computers using the simulated annealing algorithm, the results are astonishing. CNN computers could be faster than digital computers already at 10×10 lattice sizes. The local control of the template parameters was already partially realized on some of the hardwares, we think this study could further motivate their development in this direction.

  17. A MULTI CRITERIA APPROACH TO DESIGNING THE CELLULAR MANUFACTURING SYSTEM

    Directory of Open Access Journals (Sweden)

    Rika Ampuh Hadiguna

    2005-01-01

    Full Text Available Cellular manufacturing system design problems such as design framework, manufacturing cells layout and layout evaluation. The research objective is developing the framework to designing manufacturing cells with considering the organization and management aspects in shopfloor. In this research have compared the existing layout with proposed layout which applied the multi criteria approach. The proposed method is combining Analytical Hierarchy Process (AHP, Clustering and heuristic approach. The result has show that grouping with Single Linkage Clustering (SLC to be selected as manufacturing cells. The comparison of clustering weight is 0,567, 0,245 and 0,188 for SLC, Complete Linkage Clustering (CLC and Average Linkage Clustering (ALC, respectively. This result shows that generating layout by using grouping result from SLC. The evaluation result shows that types of manufacturing cells better than process layout which used the existing system.

  18. Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts.

    Science.gov (United States)

    Loh, Ken H; Stawski, Philipp S; Draycott, Austin S; Udeshi, Namrata D; Lehrman, Emily K; Wilton, Daniel K; Svinkina, Tanya; Deerinck, Thomas J; Ellisman, Mark H; Stevens, Beth; Carr, Steven A; Ting, Alice Y

    2016-08-25

    Cellular compartments that cannot be biochemically isolated are challenging to characterize. Here we demonstrate the proteomic characterization of the synaptic clefts that exist at both excitatory and inhibitory synapses. Normal brain function relies on the careful balance of these opposing neural connections, and understanding how this balance is achieved relies on knowledge of their protein compositions. Using a spatially restricted enzymatic tagging strategy, we mapped the proteomes of two of the most common excitatory and inhibitory synaptic clefts in living neurons. These proteomes reveal dozens of synaptic candidates and assign numerous known synaptic proteins to a specific cleft type. The molecular differentiation of each cleft allowed us to identify Mdga2 as a potential specificity factor influencing Neuroligin-2's recruitment of presynaptic neurotransmitters at inhibitory synapses. PMID:27565350

  19. Integration of Neural Networks and Cellular Automata for Urban Planning

    Institute of Scientific and Technical Information of China (English)

    Anthony Gar-on Yeh; LI Xia

    2004-01-01

    This paper presents a new type of cellular automata (CA) model for the simulation of alternative land development using neural networks for urban planning. CA models can be regarded as a planning tool because they can generate alternative urban growth. Alternative development patterns can be formed by using different sets of parameter values in CA simulation. A critical issue is how to define parameter values for realistic and idealized simulation. This paper demonstrates that neural networks can simplify CA models but generate more plausible results. The simulation is based on a simple three-layer network with an output neuron to generate conversion probability. No transition rules are required for the simulation. Parameter values are automatically obtained from the training of network by using satellite remote sensing data. Original training data can be assessed and modified according to planning objectives. Alternative urban patterns can be easily formulated by using the modified training data sets rather than changing the model.

  20. Cellular response to titanium discs coated with polyelectrolyte multilayer films

    Institute of Scientific and Technical Information of China (English)

    Jing Zhan; Qiao-jie Luo; Ying Huang; Xiao-dong Li

    2014-01-01

    The purpose of this study was to investigate the effects of polyelectrolyte multilayer (PEM) coatings on the biological behavior of titanium (Ti) substrates. Collagen typeΙ/hyaluronic acid (Col/HA) and chitosan/hyaluronic acid (Chi/HA) multilayer PEM coatings were in-troduced onto Ti substrates using layer-by-layer assembly. Contact angle instruments and quartz crystal microbalance were used for film characterization. The results obtained showed that both Col/HA and Chi/HA surfaces had high hydrophilicity and promoted cell adhesion in MC3T3-E1 pre-osteoblast and human gingival fibroblast cells. In addition, the synthesis of function-related proteins and gene expression levels in both MC3T3-E1 and fibroblast cells was higher for the Col/HA coating compared with the Chi/HA coating, indicating better cellu-lar response to the Col/HA coating.

  1. Receptor Oligomerization as a Process Modulating Cellular Semiotics

    DEFF Research Database (Denmark)

    Giorgi, Franco; Bruni, Luis Emilio; Maggio, Roberto

    2010-01-01

    The majority of G protein-coupled receptors (GPCRs) self-assemble in the form dimeric/oligomeric complexes along the plasma membrane. Due to the molecular interactions they participate, GPCRs can potentially provide the framework for discriminating a wide variety of intercellular signals, as based...... at which selective categorical sensing may occur. Categorical sensing can be seen as the cellular capacity for identifying and ordering complex patterns of mixed signals out of a contextual matrix, i.e., the recognition of meaningful patterns out of ubiquitous signals. In this context, redundancy...... be another level of quality control that may help maintaining GPCRs rather stable throughout evolution. We propose here receptor oligomerization to be a basic molecular mechanism controlling GPCRs redundancy in many different cell types, and the plasma membrane as the first hierarchical cell structure...

  2. Energy conservation and contribution to the sustained development through action in the bioclimate design; Ahorro de energia y contribucion al desarrollo sustentable a partir de acciones de diseno bioclimatico

    Energy Technology Data Exchange (ETDEWEB)

    Garcia Chavez, J. R.; Dorantes Rodriguez, R. [Universidad Autonoma Metropolitana, Unidad Azcapotzalco, Mexico, D. F. (Mexico)

    1995-12-31

    , construction and monitoring of the functioning of cooling passive systems. The results obtained to date point out that the energy consumption has diminished and improved the environmental comfort conditions for the library building users. [Espanol] La crisis mundial de energia de 1973, ademas de los problemas de abastecimiento y elevacion de precios, propicio tambien diversos paises, una reaccion orientada al uso eficiente y conservacion de la energia, y a la aplicacion de una arquitectura con sentido comun: la ARQUITECTURA BIOCLIMATICA, misma que se basa en la utilizacion en las edificaciones de las energias alternativas, tales como la solar eolica, etc. Obtencion de condiciones de confort natural ambiental y ahorro y uso eficiente de la energia en los edificios, son los objetivos fundamentales de la ARQUITECTURA BIOCLIMATICA. Bajo estas consideraciones, se han planteado diversos sistemas pasivos para control termico de edificios, los cuales se han aplicado en diferentes regiones climaticas del planeta. En el presente trabajo, se presenta y analiza el potencial de medidas para ahorro de energia, tales como sistemas de iluminacion artificial de alta eficiencia y sistemas pasivos de enfriamiento para el control termico de un edificio educativo, la biblioteca central de la Universidad Autonoma Metropolitana, Unidad Azcapotzalco, ubicada en la ciudad de Mexico. El objetivo de este estudio se orienta a la reduccion del consumo de energia en la biblioteca, y a la obtencion de condiciones de confort ambiental higrotermico para sus ocupantes. Estudios realizados durante la primera etapa de investigacion, han permitido identificar un excesivo consumo de energia y condiciones de falta de confort higrotermico y de la necesaria calidad del aire interior en la biblioteca. Estas acciones se han enfocado en dos aspectos. El primero se refiere al mejoramiento de la operacion de la instalacion electrica y el alumbrado de la biblioteca. Estas acciones se basaron en la sustitucion de lamparas

  3. Differential Modulation of Cellular Bioenergetics by Poly(L-lysine)s of Different Molecular Weights

    DEFF Research Database (Denmark)

    Hall, Arnaldur; Wu, Lin-Ping; Parhamifar, Ladan;

    2015-01-01

    Seahorse XF technology, we studied the impact of PLL size on cellular bioenergetic processes in two human cell lines. In contrast to L-PLLs (1-5 kDa), H-PLLs (15-30 kDa) were more detrimental to both mitochondrial oxidative phosphorylation (OXPHOS) and glycolytic activity resulting in considerable...... intracellular ATP depletion, thereby initiating necrotic-type cell death. The cellular differences to polycation sensitivity were further related to the mitochondrial state, where the impact was substantial on cells with hyperpolarized mitochondria. These medium-throughput approaches offer better opportunities...

  4. Zero-Outage Cellular Downlink with Fixed-Rate D2D Underlay

    DEFF Research Database (Denmark)

    Kiilerich Pratas, Nuno; Popovski, Petar

    2015-01-01

    Two of the emerging trends in wireless cellular systems are Device-to-Device (D2D) and Machine-to-Machine (M2M) communications. D2D enables efficient reuse of the licensed spectrum to support localized transmissions, while M2M connections are often characterized by fixed and low transmission rates....... D2D connections can be instrumental in localized aggregation of uplink M2M traffic to a more capable cellular device, before being finally delivered to the Base Station (BS). In this paper we show that a fixed M2M rate is an enabler of efficient Machine-Type D2D underlay operation taking place...

  5. Molecular physiology of cellular glucose transport - a potential area for clinical studies in diabetes mellitus.

    Science.gov (United States)

    Tatoń, Jan; Piatkiewicz, Paweł; Czech, Anna

    2010-01-01

    The normalization of cellular glucose assimilation is the basic aim of metabolic therapy in type 2 diabetes mellitus (T2DM). It requires parallel changes in the process of cellular glucose transport (CGT). This review presents the pathophysiological and clinical outlines of CGT. Sequentially, the advances in the mechanisms and classification of CGT and their physiological and molecular base are described. The role of CGT pathogenetic significance in diabetes mellitus is stressed. Finally, the opinion is expressed that the CGT study is a potentially important approach to clinical interpretation of glucose metabolism disturbances and their pharmacotherapy. PMID:20602306

  6. A Novel Approach for Attacks Mitigation in Mobile Ad Hoc Networks Using Cellular Automatas

    Directory of Open Access Journals (Sweden)

    Himadri Nath Saha

    2012-04-01

    Full Text Available Many security schemes for mobile ad-hoc network(MANET have been proposed so far but none of them has been successful in combating the different types of attacks that a mobile ad-hoc network often faces. This paper is providing one way of mitigating attacks in mobile ad-hoc networks by authenticating the node who tries to access this network .This scheme has been applied by using cellular automata (CA. Our simulation results show how cellular automata(CA is implemented for user authentication and secure transmission in MANET.

  7. Mapping of cellular iron using hyperspectral fluorescence imaging in a cellular model of Parkinson's disease

    Science.gov (United States)

    Oh, Eung Seok; Heo, Chaejeong; Kim, Ji Seon; Lee, Young Hee; Kim, Jong Min

    2013-05-01

    Parkinson's disease (PD) is characterized by progressive dopaminergic cell loss in the substantianigra (SN) and elevated iron levels demonstrated by autopsy and with 7-Tesla magnetic resonance imaging. Direct visualization of iron with live imaging techniques has not yet been successful. The aim of this study is to visualize and quantify the distribution of cellular iron using an intrinsic iron hyperspectral fluorescence signal. The 1-methyl-4-phenylpyridinium (MPP+)-induced cellular model of PD was established in SHSY5Y cells. The cells were exposed to iron by treatment with ferric ammonium citrate (FAC, 100 μM) for up to 6 hours. The hyperspectral fluorescence imaging signal of iron was examined usinga high- resolution dark-field optical microscope system with signal absorption for the visible/ near infrared (VNIR) spectral range. The 6-hour group showed heavy cellular iron deposition compared with the small amount of iron accumulation in the 1-hour group. The cellular iron was dispersed in a small, particulate form, whereas extracellular iron was detected in an aggregated form. In addition, iron particles were found to be concentrated on the cell membrane/edge of shrunken cells. The cellular iron accumulation readily occurred in MPP+-induced cells, which is consistent with previous studies demonstrating elevated iron levels in the SN in PD. This direct iron imaging methodology could be applied to analyze the physiological role of iron in PD, and its application might be expanded to various neurological disorders involving other metals, such as copper, manganese or zinc.

  8. Shape Memory Alloy-Based Periodic Cellular Structures Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This SBIR Phase II effort will continue to develop and demonstrate an innovative shape memory alloy (SMA) periodic cellular structural technology. Periodic cellular...

  9. Papillomavirus E2 Proteins and the Host Brd4 Protein Associate with Transcriptionally Active Cellular Chromatin▿ †

    OpenAIRE

    Jang, Moon Kyoo; Kwon, Deukwoo; Alison A McBride

    2009-01-01

    The interaction of papillomavirus E2 proteins with cellular Brd4 protein is important for transcriptional regulation of viral genes and partitioning of viral genomes. Bovine papillomavirus type 1 (BPV-1) E2 binds cellular chromatin in complex with Brd4 in both mitotic and interphase cells. To identify specific sites of E2 interaction on cellular chromatin, a genome-wide chromatin immunoprecipitation-on-chip analysis was carried out using human promoter sequences. Both E2 and Brd4 were found b...

  10. The Convergent Cancer Evolution toward a Single Cellular Destination.

    Science.gov (United States)

    Chen, Han; He, Xionglei

    2016-01-01

    The essence of Darwin's theory is that evolution is driven by purposeless mutations that are subsequently selected by natural environments, so there is often no predefined destination in organismal evolution. Using gene expressions of 107 cell types, we built a functional space of human cells to trace the evolutionary trajectory of 18 types of solid tumor cancers. We detected a dominant evolving trend toward the functional status of embryonic stem cells (ESC) for approximately 3,000 tumors growing in distinct tissue environments. This pattern remained the same after excluding known cancer/ESC signature genes (∼ 3,000 genes) or excluding all oncogenic gene sets (∼ 12,000 genes) annotated in MSigDB, suggesting a convergent evolution of the overall functional status in cancers. In support of this, the functional distance to ESC served as a common prognostic indicator for cancers of various types, with shorter distance corresponding to poor prognosis, which was true even when randomly selected gene sets were considered. Thus, regardless of the external environments, cancer evolution is a directional process toward a defined cellular destination, a finding reconciling development and evolution, the two seemingly incompatible philosophies both adopted by the cancer research community, and also raising new questions to evolutionary biology.

  11. Cellular proteins in influenza virus particles.

    Directory of Open Access Journals (Sweden)

    Megan L Shaw

    2008-06-01

    Full Text Available Virions are thought to contain all the essential proteins that govern virus egress from the host cell and initiation of replication in the target cell. It has been known for some time that influenza virions contain nine viral proteins; however, analyses of other enveloped viruses have revealed that proteins from the host cell can also be detected in virions. To address whether the same is true for influenza virus, we used two complementary mass spectrometry approaches to perform a comprehensive proteomic analysis of purified influenza virus particles. In addition to the aforementioned nine virus-encoded proteins, we detected the presence of 36 host-encoded proteins. These include both cytoplasmic and membrane-bound proteins that can be grouped into several functional categories, such as cytoskeletal proteins, annexins, glycolytic enzymes, and tetraspanins. Interestingly, a significant number of these have also been reported to be present in virions of other virus families. Protease treatment of virions combined with immunoblot analysis was used to verify the presence of the cellular protein and also to determine whether it is located in the core of the influenza virus particle. Immunogold labeling confirmed the presence of membrane-bound host proteins on the influenza virus envelope. The identification of cellular constituents of influenza virions has important implications for understanding the interactions of influenza virus with its host and brings us a step closer to defining the cellular requirements for influenza virus replication. While not all of the host proteins are necessarily incorporated specifically, those that are and are found to have an essential role represent novel targets for antiviral drugs and for attenuation of viruses for vaccine purposes.

  12. From Cellular Mechanotransduction to Biologically Inspired Engineering

    Science.gov (United States)

    Ingber, Donald E.

    2010-01-01

    This article is based on a lecture I presented as the recipient of the 2009 Pritzker Distinguished Lecturer Award at the Biomedical Engineering Society annual meeting in October 2009. Here, I review more than thirty years of research from my laboratory, beginning with studies designed to test the theory that cells use tensegrity (tensional integrity) architecture to stabilize their shape and sense mechanical signals, which I believed to be critical for control of cell function and tissue development. Although I was trained as a cell biologist, I found that the tools I had at my disposal were insufficient to experimentally test these theories, and thus I ventured into engineering to find critical solutions. This path has been extremely fruitful as it has led to confirmation of the critical role that physical forces play in developmental control, as well as how cells sense and respond to mechanical signals at the molecular level through a process known as cellular mechanotransduction. Many of the predictions of the cellular tensegrity model relating to cell mechanical behaviors have been shown to be valid, and this vision of cell structure led to discovery of the central role that transmembrane adhesion receptors, such as integrins, and the cytoskeleton play in mechanosensing and mechanochemical conversion. In addition, these fundamental studies have led to significant unexpected technology fallout, including development of micromagnetic actuators for non-invasive control of cellular signaling, microfluidic systems as therapeutic extracorporeal devices for sepsis therapy, and new DNA-based nanobiotechnology approaches that permit construction of artificial tensegrities that mimic properties of living materials for applications in tissue engineering and regenerative medicine. PMID:20140519

  13. Cellular heterogeneity and live cell arrays.

    Science.gov (United States)

    Walling, Maureen A; Shepard, Jason R E

    2011-07-01

    In the past decade, the tendency to move from a global, one-size-fits-all treatment philosophy to personalized medicine is based, in part, on the nuanced differences and sub-classifications of disease states. Our knowledge of these varied states stems from not only the ability to diagnose, classify, and perform experiments on cell populations as a whole, but also from new technologies that allow interrogation of cell populations at the individual cell level. Such departures from conventional thinking are driven by the recognition that clonal cell populations have numerous activities that manifest as significant levels of non-genetic heterogeneity. Clonal populations by definition originate from a single genetic origin so are regarded as having a high level of homogeneity as compared to genetically distinct cell populations. However, analysis at the single cell level has revealed a different phenomenon; cells and organisms require an inherent level of non-genetic heterogeneity to function properly, and in some cases, to survive. The growing understanding of this occurrence has lead to the development of methods to monitor, analyze, and better characterize the heterogeneity in cell populations. Following the trend of DNA- and protein microarrays, platforms capable of simultaneously monitoring each cell in a population have been developed. These cellular microarray platforms and other related formats allow for continuous monitoring of single live cells and simultaneously generate individual cell and average population data that are more descriptive and information-rich than traditional bulk methods. These technological advances have helped develop a better understanding of the intricacies associated with biological processes and afforded greater insight into complex biological systems. The associated instruments, techniques, and reagents now allow for highly multiplexed analyses, which enable multiple cellular activities, processes, or pathways to be monitored

  14. Microwave components for cellular portable radiotelephone

    Science.gov (United States)

    Muraguchi, Masahiro; Aikawa, Masayoshi

    1995-09-01

    Mobile and personal communication systems are expected to represent a huge market for microwave components in the coming years. A number of components in silicon bipolar, silicon Bi-CMOS, GaAs MESFET, HBT and HEMT are now becoming available for system application. There are tradeoffs among the competing technologies with regard to performance, cost, reliability and time-to-market. This paper describes process selection and requirements of cost and r.f. performances to microwave semiconductor components for digital cellular and cordless telephones. Furthermore, new circuit techniques which were developed by NTT are presented.

  15. Imaging Cellular Architecture with X-rays

    OpenAIRE

    Larabell, Carolyn A.; Nugent, Keith A.

    2010-01-01

    X-ray imaging of biological samples is progressing rapidly. In this paper we review the progress to date in high resolution imaging of cellular architecture. In particular we survey the progress in soft X-ray tomography and argue that the field is coming of age and that important biological insights are starting to emerge. We then review the new ideas based on coherent diffraction. These methods are at a much earlier stage of development but, as they eliminate the need for X-ray optics, have ...

  16. Cellular and physical mechanisms of branching morphogenesis

    Science.gov (United States)

    Varner, Victor D.; Nelson, Celeste M.

    2014-01-01

    Branching morphogenesis is the developmental program that builds the ramified epithelial trees of various organs, including the airways of the lung, the collecting ducts of the kidney, and the ducts of the mammary and salivary glands. Even though the final geometries of epithelial trees are distinct, the molecular signaling pathways that control branching morphogenesis appear to be conserved across organs and species. However, despite this molecular homology, recent advances in cell lineage analysis and real-time imaging have uncovered surprising differences in the mechanisms that build these diverse tissues. Here, we review these studies and discuss the cellular and physical mechanisms that can contribute to branching morphogenesis. PMID:25005470

  17. Wireless communications, cellular phones and relay antennas

    International Nuclear Information System (INIS)

    Wireless communications using radiofrequency fields (RF) have seen an increasing use with the massive deployment of cellular systems GSM, UMTS and now LTE. In parallel, public concerns have been raised about possible health effects. During the last decade, about 10 million Euros per year have been dedicated to research. In vivo, in vitro, animal and human studies have been carried out. This paper gives an overview of the subject. The applicable limits and regulation as well as the typical human exposure are presented. The state of knowledge on biological and health effect is summarized. The recent classification of RF by the IARC and some sociological aspects are also discussed. (authors)

  18. Cellular automata modelling of hantarvirus infection

    Energy Technology Data Exchange (ETDEWEB)

    Abdul Karim, Mohamad Faisal [School of Distance Education, Universiti Sains Malaysia, Minden 11800, Penang (Malaysia)], E-mail: faisal@usm.my; Md Ismail, Ahmad Izani [School of Mathematical Sciences, Universiti Sains Malaysia, Minden 11800, Penang (Malaysia)], E-mail: izani@cs.usm.my; Ching, Hoe Bee [School of Mathematical Sciences, Universiti Sains Malaysia, Minden 11800, Penang (Malaysia)], E-mail: Bee_Ching_Janice_Hoe@dell.com

    2009-09-15

    Hantaviruses are a group of viruses which have been identified as being responsible for the outbreak of diseases such as the hantavirus pulmonary syndrome. In an effort to understand the characteristics and dynamics of hantavirus infection, mathematical models based on differential equations have been developed and widely studied. However, such models neglect the local characteristics of the spreading process and do not include variable susceptibility of individuals. In this paper, we develop an alternative approach based on cellular automata to analyze and study the spatiotemporal patterns of hantavirus infection.

  19. Single spin measurement using cellular automata techniques

    CERN Document Server

    Perez-Delgado, C A; Cory, D G; Mosca, M; Cappellaro, Paola; Cory, David G.; Mosca, Michele; Perez-Delgado, Carlos A.

    2006-01-01

    We propose an approach for single spin measurement. Our method uses techniques from the theory of quantum cellular automata to correlate a large amount of ancillary spins to the one to be measured. It has the distinct advantage of being efficient, and to a certain extent fault-tolerant. Under ideal conditions, it requires the application of only order of cube root of N steps (each requiring a constant number of rf pulses) to create a system of N correlated spins. It is also fairly robust against pulse errors, imperfect initial polarization of the ancilla spin system, and does not rely on entanglement. We study the scalability of our scheme through numerical simulation.

  20. Single spin measurement using cellular automata techniques

    OpenAIRE

    Perez-Delgado, Carlos A.; Mosca, Michele; Cappellaro, Paola; Cory, David G.

    2006-01-01

    We propose an approach for single spin measurement. Our method uses techniques from the theory of quantum cellular automata to correlate a large amount of ancillary spins to the one to be measured. It has the distinct advantage of being efficient, and to a certain extent fault-tolerant. Under ideal conditions, it requires the application of only order of cube root of N steps (each requiring a constant number of rf pulses) to create a system of N correlated spins. It is also fairly robust agai...