WorldWideScience

Sample records for cellular level euclidean

  1. Energy levels of QED in a Euclidean formulation

    International Nuclear Information System (INIS)

    Hydrogen-like energy levels of scalar and spinor QED are calculated using a Euclidean functional approach. The matter field is integrated over. Stationary points of the resulting effective action already yield a hydrogen like level structure for the energy. There is an interesting difference between the scalar and the spinor case. Whereas for spinors the conventional results are reproduced, the calculation for scalars yields a fine structure which is opposite in sign to the conventional one and has no critical singularity at Zα = 1/2. The crucial structural difference between the two cases is that, for scalars, minima for the gauge invariant energy are not extrema of the action, even for time independent fields. (orig.)

  2. Repaglinide at a cellular level

    DEFF Research Database (Denmark)

    Krogsgaard Thomsen, M; Bokvist, K; Høy, M;

    2002-01-01

    To investigate the hormonal and cellular selectivity of the prandial glucose regulators, we have undertaken a series of experiments, in which we characterised the effects of repaglinide and nateglinide on ATP-sensitive potassium ion (KATP) channel activity, membrane potential and exocytosis in ra...

  3. Spacetime and Euclidean Geometry

    CERN Document Server

    Brill, D R; Brill, Dieter; Jacobson, Ted

    2004-01-01

    Using only the principle of relativity and Euclidean geometry we show in this pedagogical article that the square of proper time or length in a two-dimensional spacetime diagram is proportional to the Euclidean area of the corresponding causal domain. We use this relation to derive the Minkowski line element by two geometric proofs of the "spacetime Pythagoras theorem".

  4. Spacetime and Euclidean geometry

    Science.gov (United States)

    Brill, Dieter; Jacobson, Ted

    2006-04-01

    Using only the principle of relativity and Euclidean geometry we show in this pedagogical article that the square of proper time or length in a two-dimensional spacetime diagram is proportional to the Euclidean area of the corresponding causal domain. We use this relation to derive the Minkowski line element by two geometric proofs of the spacetime Pythagoras theorem.

  5. Euclidean Field Theory

    OpenAIRE

    Guerra, Francesco

    2005-01-01

    A coincise review about Euclidean (Quantum) Field Theory is presented. It deals with the general structural properties, the connections with Quantum Field Theory, the exploitation in Constructive Quantum Field Theory, and the physical interpretation.

  6. Non-Local Euclidean Medians.

    Science.gov (United States)

    Chaudhury, Kunal N; Singer, Amit

    2012-11-01

    In this letter, we note that the denoising performance of Non-Local Means (NLM) can be improved at large noise levels by replacing the mean by the Euclidean median. We call this new denoising algorithm the Non-Local Euclidean Medians (NLEM). At the heart of NLEM is the observation that the median is more robust to outliers than the mean. In particular, we provide a simple geometric insight that explains why NLEM performs better than NLM in the vicinity of edges, particularly at large noise levels. NLEM can be efficiently implemented using iteratively reweighted least squares, and its computational complexity is comparable to that of NLM. We provide some preliminary results to study the proposed algorithm and to compare it with NLM.

  7. The non-Euclidean revolution

    CERN Document Server

    Trudeau, Richard J

    1986-01-01

    How unique and definitive is Euclidean geometry in describing the "real" space in which we live? Richard Trudeau confronts the fundamental question of truth and its representation through mathematical models in The Non-Euclidean Revolution. First, the author analyzes geometry in its historical and philosophical setting; second, he examines a revolution every bit as significant as the Copernican revolution in astronomy and the Darwinian revolution in biology; third, on the most speculative level, he questions the possibility of absolute knowledge of the world. Trudeau writes in a lively, entertaining, and highly accessible style. His book provides one of the most stimulating and personal presentations of a struggle with the nature of truth in mathematics and the physical world. A portion of the book won the Pólya Prize, a distinguished award from the Mathematical Association of America. "Trudeau meets the challenge of reaching a broad audience in clever ways...(The book) is a good addition to our literature o...

  8. Non-Euclidean geometry

    CERN Document Server

    Bonola, Roberto

    2010-01-01

    This is an excellent historical and mathematical view by a renowned Italian geometer of the geometries that have risen from a rejection of Euclid's parallel postulate. Students, teachers and mathematicians will find here a ready reference source and guide to a field that has now become overwhelmingly important.Non-Euclidean Geometry first examines the various attempts to prove Euclid's parallel postulate-by the Greeks, Arabs, and mathematicians of the Renaissance. Then, ranging through the 17th, 18th and 19th centuries, it considers the forerunners and founders of non-Euclidean geometry, such

  9. Introductory non-Euclidean geometry

    CERN Document Server

    Manning, Henry Parker

    2013-01-01

    This fine and versatile introduction begins with the theorems common to Euclidean and non-Euclidean geometry, and then it addresses the specific differences that constitute elliptic and hyperbolic geometry. 1901 edition.

  10. Euclidean distance geometry and applications

    OpenAIRE

    Liberti, Leo; Lavor, Carlile; Maculan, Nelson; Mucherino, Antonio

    2012-01-01

    Euclidean distance geometry is the study of Euclidean geometry based on the concept of distance. This is useful in several applications where the input data consists of an incomplete set of distances, and the output is a set of points in Euclidean space that realizes the given distances. We survey some of the theory of Euclidean distance geometry and some of the most important applications: molecular conformation, localization of sensor networks and statics.

  11. Graviperception and graviresponse at the cellular level

    Science.gov (United States)

    Bräucker, Richard; Cogoli, Augusto; Hemmersbach, Ruth

    Studies under varied acceleration conditions demonstrated that free living cells such as protists are able to perceive changes of the acceleration conditions. Recent studies favorite the hypothesis that in these systems gravity is perceived either by intracellular receptors (statocyst-like organelles), heavy cell organelles (such as nucleus) and/or by sensing the cell mass by means of ion channels located in the cell membrane. Mammalian cells in microgravity were profoundly influenced. Alteration in the cellular mechanisms and structures in mammalian cells like signal transduction and the cytoskeleton were detected. It can be speculated that the depression of the immune system may become a serious health issue on flights to and from Mars.

  12. Analysis in Euclidean space

    CERN Document Server

    Hoffman, Kenneth

    2007-01-01

    Developed for an introductory course in mathematical analysis at MIT, this text focuses on concepts, principles, and methods. Its introductions to real and complex analysis are closely formulated, and they constitute a natural introduction to complex function theory.Starting with an overview of the real number system, the text presents results for subsets and functions related to Euclidean space of n dimensions. It offers a rigorous review of the fundamentals of calculus, emphasizing power series expansions and introducing the theory of complex-analytic functions. Subsequent chapters cover seq

  13. Euclidean shortest paths

    CERN Document Server

    Li, Fajie

    2011-01-01

    This unique text/reference reviews algorithms for the exact or approximate solution of shortest-path problems, with a specific focus on a class of algorithms called rubberband algorithms. Discussing each concept and algorithm in depth, the book includes mathematical proofs for many of the given statements. Topics and features: provides theoretical and programming exercises at the end of each chapter; presents a thorough introduction to shortest paths in Euclidean geometry, and the class of algorithms called rubberband algorithms; discusses algorithms for calculating exact or approximate ESPs i

  14. Modeling cellular deformations using the level set formalism

    OpenAIRE

    Yang Liu; Effler Janet C; Kutscher Brett L; Sullivan Sarah E; Robinson Douglas N; Iglesias Pablo A

    2008-01-01

    Abstract Background Many cellular processes involve substantial shape changes. Traditional simulations of these cell shape changes require that grids and boundaries be moved as the cell's shape evolves. Here we demonstrate that accurate cell shape changes can be recreated using level set methods (LSM), in which the cellular shape is defined implicitly, thereby eschewing the need for updating boundaries. Results We obtain a viscoelastic model of Dictyostelium cells using micropipette aspiratio...

  15. Modeling cellular deformations using the level set formalism

    Directory of Open Access Journals (Sweden)

    Yang Liu

    2008-07-01

    Full Text Available Abstract Background Many cellular processes involve substantial shape changes. Traditional simulations of these cell shape changes require that grids and boundaries be moved as the cell's shape evolves. Here we demonstrate that accurate cell shape changes can be recreated using level set methods (LSM, in which the cellular shape is defined implicitly, thereby eschewing the need for updating boundaries. Results We obtain a viscoelastic model of Dictyostelium cells using micropipette aspiration and show how this viscoelastic model can be incorporated into LSM simulations to recreate the observed protrusion of cells into the micropipette faithfully. We also demonstrate the use of our techniques by simulating the cell shape changes elicited by the chemotactic response to an external chemoattractant gradient. Conclusion Our results provide a simple but effective means of incorporating cellular deformations into mathematical simulations of cell signaling. Such methods will be useful for simulating important cellular events such as chemotaxis and cytokinesis.

  16. Three-level description of the domino cellular automaton

    CERN Document Server

    Czechowski, Zbigniew

    2010-01-01

    Inspired by the approach of kinetic theory of gases, a three-level description (microscopic, mesoscopic and macroscopic) of cellular automaton is presented. To provide an analytical treatment a simple domino cellular automaton with avalanches was constructed. Formulas concerning exact relations for density, clusters, avalanches and other parameters in an equilibrium state were derived. It appears that some relations are approximately valid for deviations from the equilibrium, so the adequate Ito equation could be constructed. The equation provides the time evolution description of some variable on the macroscopic level. The results also suggest a motive for applying of the procedure of construction of the Ito equation (from time series data) to natural time series.

  17. Body composition analysis: Cellular level modeling of body component ratios

    OpenAIRE

    Z. Wang; Heymsfield, S. B.; PI-SUNYER, F.X.; Gallagher, D.; PIERSON, R.N.

    2008-01-01

    During the past two decades, a major outgrowth of efforts by our research group at St. Luke’s-Roosevelt Hospital is the development of body composition models that include cellular level models, models based on body component ratios, total body potassium models, multi-component models, and resting energy expenditure-body composition models. This review summarizes these models with emphasis on component ratios that we believe are fundamental to understanding human body composition during growt...

  18. Ideas of Space - Euclidean, Non-Euclidean, and Relativistic

    Science.gov (United States)

    Gray, Jeremy

    1989-09-01

    Now in a revised and expanded new edition, this volume chronologically traces the evolution of Euclidean, non-Euclidean, and relativistic theories regarding the shape of the universe. A unique, highly readable, and entertaining account, the book assumes no special mathematical knowledge. It reviews the failed classical attempts to prove the parallel postulate and provides coverage of the role of Gauss, Lobachevskii, and Bolyai in setting the foundations of modern differential geometry, which laid the groundwork for Einstein's theories of special and general relativity. This updated account includes a new chapter on Islamic contributions to this area, as well as additional information on gravitation, the nature of space and black holes.

  19. Concepts of dose to soft tissue at the cellular level

    International Nuclear Information System (INIS)

    Radiation effects begin at the cellular level of biological organization. Radiation dosimetry at the cellular level is particularly important for internally deposited alpha and beta particle emitters. Microdosimetry is a mechanism for studying the dose imparted to microscopic sites, for determining hit probabilities, and for determining the probability that sites are missed. Internal microdosimetry calculations are complex, but can be easily executed using computer programs. The investigator must specify the target and its size, determine the radionuclide activity per unit mass for each region in which targets are located, describe the activity per radioactive particulate, understand the geometrical relationship between the activity and the targets, and account for the biological retention of the activity in the region as a function of time. Internal microdosimetry has many potential applications in radiological protection. Microdosimetry is a special research area designed to provide a better understanding of the importance of microscopic patterns of radiation interaction with cells within the broader framework of biochemistry and radiation biology. Its objective is to provide a methodology that is both consistent and precise for correlating biological response to varying levels and distributions of internal emitters. Microdosimetry may contribute to a more complete understanding of the mechanisms of cancer induction by radiation. The correlation between specific energy density and various biological effects might best be treated statistically, since the effects occur in response of stochastic processes. If applied correctly, these concepts should provide a reliable tool for learning more about the effects of radiation and for setting radiation protection standards

  20. Electrochemical Biochip for Drug Screening At Cellular Level

    Energy Technology Data Exchange (ETDEWEB)

    Chen Yu [Institute of Microelectronics, 11 Science Park Road, Science Park II, Singapore 117685 (Singapore); Cui Huiang [Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543 (Singapore); Ye Jianshan [Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543 (Singapore); Chong Serchoong [Institute of Microelectronics, 11 Science Park Road, Science Park II, Singapore 117685 (Singapore); Lim Titmeng [Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543 (Singapore); Sheu Fwushan [Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543 (Singapore); Hui Wingcheong [Institute of Microelectronics, 11 Science Park Road, Science Park II, Singapore 117685 (Singapore)

    2006-04-01

    Drug screening at cellular level has becomes an attractive field of research. Different researchers have tried to record cellular response to drugs by electrical or optical approach using both invasive and non-invasive methods. Silicon-based microelectrode integrated microchips are useful tools for in situ temporal recording of neurotransmitter releasing from neural cells. A microfabricated electrochemical biochip is presented in this paper. Using dopaminergic cells grown on the chip, the dopamine excytosis can be electrochemical amperomatric detected non-invasively from drug incubated dopaminegic cells by the microelectrode integrated on chip. This silicon-based electrochemical chip has been designed with an electrode array located on the cell culture chamber bottom. Each electrode is individually electrical controlled. MN9D and PC12 dopaminergic cell lines have been demonstrated on this chip for drug effects study. This silicon-based electrochemical microchip provides a non-invasive, in situ, temporal detection of dopamine exocytosis from dopaminegic cells, and holds the potential for applications in studying the mechanisms of dopamine exocytosis and drug screening. It is also extendable for other cell culture and drug effects study.

  1. Cellular chromophores and signaling in low level light therapy

    Science.gov (United States)

    Hamblin, Michael R.; Demidova-Rice, Tatiana N.

    2007-02-01

    The use of low levels of visible or near infrared light (LLLT) for reducing pain, inflammation and edema, promoting healing of wounds, deeper tissues and nerves, and preventing tissue damage by reducing cellular apoptosis has been known for almost forty years since the invention of lasers. Originally thought to be a peculiar property of laser light (soft or cold lasers), the subject has now broadened to include photobiomodulation and photobiostimulation using non-coherent light. Despite many reports of positive findings from experiments conducted in vitro, in animal models and in randomized controlled clinical trials, LLLT remains controversial. This likely is due to two main reasons; firstly the biochemical mechanisms underlying the positive effects are incompletely understood, and secondly the complexity of rationally choosing amongst a large number of illumination parameters such as wavelength, fluence, power density, pulse structure and treatment timing has led to the publication of a number of negative studies as well as many positive ones. In recent years major advances have been made in understanding the mechanisms that operate at the cellular and tissue levels during LLLT. Mitochondria are thought to be the main site for the initial effects of light and specifically cytochrome c oxidase that has absorption peaks in the red and near infrared regions of the electromagnetic spectrum matches the action spectra of LLLT effects. The discovery that cells employ nitric oxide (NO) synthesized in the mitochondria by neuronal nitric oxide synthase, to regulate respiration by competitive binding to the oxygen binding of cytochrome c oxidase, now suggests how LLLT can affect cell metabolism. If LLLT photodissociates inhibitory NO from cytochrome c oxidase, this would explain increased ATP production, modulation of reactive oxygen species, reduction and prevention of apoptosis, stimulation of angiogenesis, increase of blood flow and induction of transcription factors. In

  2. Fast Exact Euclidean Distance (FEED) Transformation

    NARCIS (Netherlands)

    Schouten, Theo; Broek, van den Egon; Kittler, J.; Petrou, M.; Nixon, M.

    2004-01-01

    Fast Exact Euclidean Distance (FEED) transformation is introduced, starting from the inverse of the distance transformation. The prohibitive computational cost of a naive implementation of traditional Euclidean Distance Transformation, is tackled by three operations: restriction of both the number o

  3. Euclidean Circles and Their Modular Images.

    Science.gov (United States)

    Austin, Joe Dan

    1990-01-01

    Shows a series of Euclidean equations using the Euclidean algorithm to get the greatest common divisor of two integers. Describes the use of the equations to generate a series of circles. Discusses computer generation of Euclidean circles and provides a BASIC program. (YP)

  4. An axiomatization of the Euclidean compromise solution

    NARCIS (Netherlands)

    Voorneveld, M.; Nouweland, A. van den

    2001-01-01

    The Euclidean compromise solution in multicriteria optimization is a solution concept that assigns to a feasible set the alternative with minimal Euclidean distance to the utopia point The purpose of this paper is to provide a characterization of the Euclidean compromise solution

  5. Introduction to non-Euclidean geometry

    CERN Document Server

    Wolfe, Harold E

    2012-01-01

    One of the first college-level texts for elementary courses in non-Euclidean geometry, this concise, readable volume is geared toward students familiar with calculus. A full treatment of the historical background explores the centuries-long efforts to prove Euclid's parallel postulate and their triumphant conclusion. Numerous original exercises form an integral part of the book.Topics include hyperbolic plane geometry and hyperbolic plane trigonometry, applications of calculus to the solutions of some problems in hyperbolic geometry, elliptic plane geometry and trigonometry, and the consistenc

  6. Effects of high let radiation at the cellular level

    International Nuclear Information System (INIS)

    The complete evaluation of the impact of nuclear energy technologies on human health requires: identification and characterization of hazardous agents and their effects, which is well advanced; quantitative dose-effect relations for important effects under various conditions of radiation exposure and biological variation, which needs more work but which has been advancing fairly well, particularly in experimental animal research; an understanding of the cellular and tissue mechanisms of the hazardous effects in relation to the microdosimetry of the agents, which has advanced slowly and to a relatively limited degree; and integration of the dose-effect relations and mechanistic knowledge into theories and models which are intended to be predictive for estimation of human health risks at pertinent low levels of exposure. Several theories, hypotheses or models of radiation carcinogenesis, and some for alpha radiation in particular, have been developed on the basis of dose-effect relations in the necessary attempt to predict the hazards of low levels of radiation exposure for purposes of radiation protection in advance of adequate data on dose-effect relations and mchanisms. As such these theories or models have necessarily involved strings of explicit and implicit assumptions concerning mechanisms and the nature of dose-effect relations below the dose regions at which effects have been observed. Nevertheless, they have been useful in stimulating research to test their assumptions and in assuming the interpretation of data

  7. Euclidean geometry and its subgeometries

    CERN Document Server

    Specht, Edward John; Calkins, Keith G; Rhoads, Donald H

    2015-01-01

    In this monograph, the authors present a modern development of Euclidean geometry from independent axioms, using up-to-date language and providing detailed proofs. The axioms for incidence, betweenness, and plane separation are close to those of Hilbert. This is the only axiomatic treatment of Euclidean geometry that uses axioms not involving metric notions and that explores congruence and isometries by means of reflection mappings. The authors present thirteen axioms in sequence, proving as many theorems as possible at each stage and, in the process, building up subgeometries, most notably the Pasch and neutral geometries. Standard topics such as the congruence theorems for triangles, embedding the real numbers in a line, and coordinatization of the plane are included, as well as theorems of Pythagoras, Desargues, Pappas, Menelaus, and Ceva. The final chapter covers consistency and independence of axioms, as well as independence of definition properties. There are over 300 exercises; solutions to many of the...

  8. Finite, primitive and euclidean spaces

    Directory of Open Access Journals (Sweden)

    Efim Khalimsky

    1988-01-01

    Full Text Available Integer and digital spaces are playing a significant role in digital image processing, computer graphics, computer tomography, robot vision, and many other fields dealing with finitely or countable many objects. It is proven here that every finite T0-space is a quotient space of a subspace of some simplex, i.e. of some subspace of a Euclidean space. Thus finite and digital spaces can be considered as abstract simplicial structures of subspaces of Euclidean spaces. Primitive subspaces of finite, digital, and integer spaces are introduced. They prove to be useful in the investigation of connectedness structure, which can be represented as a poset, and also in consideration of the dimension of finite spaces. Essentially T0-spaces and finitely connected and primitively path connected spaces are discussed.

  9. Hausdorff operators on Euclidean spaces

    Institute of Scientific and Technical Information of China (English)

    CHEN Jie-cheng; FAN Da-shan; WANG Si-lei

    2013-01-01

    Hausdorff operator is an important operator raised from the dilation on Euclidean space and rooted in the classical summability of number series and Fourier series. It is also connected to many well known operators in real and complex analysis. This article is a survey of some recent developments and extensions on the Hausdorff operator. Particularly, various boundedness properties of the Hausdorff operators, studied recently by our research group, are addressed.

  10. Hardware Euclidean Distance Transform Implementation

    Institute of Scientific and Technical Information of China (English)

    XIE Ying-hua; ZENG Pei-feng; TANG Li-ping

    2004-01-01

    Based on an efficient algorithm of Euclidean distance transform for binary images, a circuit of O(N2) size is proposed. With in-place calculation, both the intermediate data storing and the result output use the same memory with the input data. This reduces the amount of memory largely. By replacing multipliers with counters, comparators, and adders, the circuit size is further reduced and its calculation speed is improved also.

  11. Conformal Janus on Euclidean Sphere

    CERN Document Server

    Bak, Dongsu; Rey, Soo-Jong

    2016-01-01

    We interpret Janus as an interface in a conformal field theory and study its properties. The Janus is created by an exactly marginal operator and we study its effect on the interface conformal field theory on the Janus. We do this by utilizing the AdS/CFT correspondence. We compute the interface free energy both from leading correction to the Euclidean action in the dual gravity description and from conformal perturbation theory in the conformal field theory. We find that the two results agree each other and that the interface free energy scales precisely as expected from the conformal invariance of the Janus interface.

  12. Cellular identity at the single-cell level.

    Science.gov (United States)

    Coskun, Ahmet F; Eser, Umut; Islam, Saiful

    2016-10-20

    A single cell creates surprising heterogeneity in a multicellular organism. While every organismal cell shares almost an identical genome, molecular interactions in cells alter the use of DNA sequences to modulate the gene of interest for specialization of cellular functions. Each cell gains a unique identity through molecular coding across the DNA, RNA, and protein conversions. On the other hand, loss of cellular identity leads to critical diseases such as cancer. Most cell identity dissection studies are based on bulk molecular assays that mask differences in individual cells. To probe cell-to-cell variability in a population, we discuss single cell approaches to decode the genetic, epigenetic, transcriptional, and translational mechanisms for cell identity formation. In combination with molecular instructions, the physical principles behind cell identity determination are examined. Deciphering and reprogramming cellular types impact biology and medicine.

  13. Euclidean Prize-collecting Steiner Forest

    OpenAIRE

    Bateni, MohammadHossein; Hajiaghayi, Mohammadtaghi

    2009-01-01

    In this paper, we consider Steiner forest and its generalizations, prize-collecting Steiner forest and k-Steiner forest, when the vertices of the input graph are points in the Euclidean plane and the lengths are Euclidean distances. First, we present a simpler analysis of the polynomial-time approximation scheme (PTAS) of Borradaile et al. [12] for the Euclidean Steiner forest problem. This is done by proving a new structural property and modifying the dynamic programming by adding a new piec...

  14. Cellular level loading and heating of superparamagnetic iron oxide nanoparticles.

    Science.gov (United States)

    Kalambur, Venkat S; Longmire, Ellen K; Bischof, John C

    2007-11-20

    Superparamagnetic iron oxide nanoparticles (NPs) hold promise for a variety of biomedical applications due to their properties of visualization using magnetic resonance imaging (MRI), heating with radio frequency (rf), and movement in an external magnetic field. In this study, the cellular loading (uptake) mechanism of dextran- and surfactant-coated iron oxide NPs by malignant prostate tumor cells (LNCaP-Pro5) has been studied, and the feasibility of traditional rf treatment and a new laser heating method was evaluated. The kinetics of cell loading was quantified using magnetophoresis and a colorimetric assay. The results showed that loading of surfactant-coated iron oxide NPs with LNCaP-Pro5 was saturable with time (at 24 h) and extracellular concentration (11 pg Fe/cell at 0.5 mg Fe/mL), indicating that the particles are taken up by an "adsorptive endocytosis" pathway. Dextran-coated NPs, however, were taken up less efficiently (1 pg Fe/cell at 0.5 mg Fe/mL). Loading did not saturate with concentration suggesting uptake by fluid-phase endocytosis. Magnetophoresis suggests that NP-loaded cells can be held using external magnetic fields in microcirculatory flow velocities in vivo or in an appropriately designed extracorporeal circuit. Loaded cells were heated using traditional rf (260A, 357 kHz) and a new laser method (532 nm, 7 ns pulse duration, 0.03 J/pulse, 20 pulse/s). Iron oxide in water was found to absorb sufficiently strongly at 532 nm such that heating of individual NPs and thus loaded cells (1 pg Fe/cell) was effective (10 pg Fe/cell) and longer duration (30 min) when compared to laser to accomplish cell destruction (50% viability at 10 pg Fe/cell). Scaling calculations show that the pulsed laser method can lead to single-cell (loaded with NPs) treatments (200 degrees C temperature change at the surface of an individual NP) unlike traditional rf heating methods which can be used only for bulk tissue level treatments. In a mixture of normal and NP

  15. Notes on euclidean de Sitter space

    International Nuclear Information System (INIS)

    We discuss issues relating to the topology of euclidean de Sitter space. We show that in (2+1) dimensions, the euclidean continuation of the 'causal diamond', i.e. the region of spacetime accessible to a timelike observer is a three-hemisphere. However, when de Sitter entropy is computed in a 'stretched horizon' picture, then we argue that the correct euclidean topology is a solid torus. The solid torus shrinks and degenerates into a three-hemisphere as one goes from the 'stretched horizon' to the horizon, giving the euclidean continuation of the causal diamond. We finally comment on generalisation of these results to higher dimensions. (author)

  16. Einstein Constraints on Asymptotically Euclidean Manifolds

    CERN Document Server

    Choquet-Bruhat, Y; York, J W; Choquet-Bruhat, Yvonne; Isenberg, James; York, James W.

    2000-01-01

    We consider the Einstein constraints on asymptotically euclidean manifolds $M$ of dimension $n \\geq 3$ with sources of both scaled and unscaled types. We extend to asymptotically euclidean manifolds the constructive method of proof of existence. We also treat discontinuous scaled sources. In the last section we obtain new results in the case of non-constant mean curvature.

  17. Human more complex than mouse at cellular level.

    Directory of Open Access Journals (Sweden)

    Alexander E Vinogradov

    Full Text Available The family of transcription factors with the C2H2 zinc finger domain is expanding in the evolution of vertebrates, reaching its highest numbers in the mammals. The question arises: whether an increased amount of these transcription factors is related to embryogenesis, nervous system, pathology or more of them are expressed in individual cells? Among mammals, the primates have a more complex anatomical structure than the rodents (e.g., brain. In this work, I show that a greater number of C2H2-ZF genes are expressed in the human cells than in the mouse cells. The effect is especially pronounced for C2H2-ZF genes accompanied with the KRAB domain. The relative difference between the numbers of C2H2-ZF(-KRAB genes in the human and mouse cellular transcriptomes even exceeds their difference in the genomes (i.e. a greater subset of existing in the genome genes is expressed in the human cellular transcriptomes compared to the mouse transcriptomes. The evolutionary turnover of C2H2-ZF(-KRAB genes acts in the direction of the revealed phenomenon, i.e. gene duplication and loss enhances the difference in the relative number of C2H2-ZF(-KRAB genes between human and mouse cellular transcriptomes. A higher amount of these genes is expressed in the brain and embryonic cells (compared with other tissues, whereas a lower amount--in the cancer cells. It is specifically the C2H2-ZF transcription factors whose repertoire is poorer in the cancer and richer in the brain (other transcription factors taken together do not show this trend. These facts suggest that increase of anatomical complexity is accompanied by a more complex intracellular regulation involving these transcription factors. Malignization is associated with simplification of this regulation. These results agree with the known fact that human cells are more resistant to oncogenic transformation than mouse cells. The list of C2H2-ZF genes whose suppression might be involved in malignization is provided.

  18. Using a Virtual Tissue Culture System to Assist Students in Understanding Life at the Cellular Level

    Science.gov (United States)

    McLauglin, Jacqueline S.; Seaquist, Stephen B.

    2008-01-01

    In every biology course ever taught in the nation's classrooms, and in every biology book ever published, students are taught about the "cell." The cell is as fundamental to biology as the atom is to chemistry. Truly, everything an organism does occurs fundamentally at the cellular level. Beyond memorizing the cellular definition, students are not…

  19. β-Amyloid pathogenesis: Chemical properties versus cellular levels

    DEFF Research Database (Denmark)

    Tiwari, Manish Kumar; Kepp, Kasper Planeta

    2016-01-01

    , or aggregation propensities. Cytotoxicity correlates inversely with total Aβ42 (R2=0.65, P =.016) and Aβ42/Aβ40 ratios (R2=0.76, P=.005), i.e., chemical properties that increase Aβ42 also reduce toxicity. The complexity and heterogeneity of data reveal the need to understand these phenotypes better, e......Although genetic Aβ variants cause early-onset Alzheimer's disease, literature reports on Aβ properties are heterogeneous, obscuring molecular mechanisms, as illustrated by recent failures of Aβ-level targeting trials. Thus, we combined available data on Aβ levels and ratios, aggregation...

  20. Classical geometry Euclidean, transformational, inversive, and projective

    CERN Document Server

    Leonard, I E; Liu, A C F; Tokarsky, G W

    2014-01-01

    Features the classical themes of geometry with plentiful applications in mathematics, education, engineering, and science Accessible and reader-friendly, Classical Geometry: Euclidean, Transformational, Inversive, and Projective introduces readers to a valuable discipline that is crucial to understanding bothspatial relationships and logical reasoning. Focusing on the development of geometric intuitionwhile avoiding the axiomatic method, a problem solving approach is encouraged throughout. The book is strategically divided into three sections: Part One focuses on Euclidean geometry, which p

  1. A Negative Mode About Euclidean Wormhole

    OpenAIRE

    Rubakov, V. A.; Shvedov, O. Yu.

    1996-01-01

    Wormholes -- solutions to the euclidean Einstein equations with non-trivial topology -- are usually assumed to make real contributions to amplitudes in quantum gravity. However, we find a negative mode among fluctuations about the Giddings-Strominger wormhole solution. Hence, the wormhole contribution to the euclidean functional integral is argued to be purely imaginary rather than real, which suggests the interpretation of the wormhole as describing the instability of a large universe agains...

  2. The Euclidean distribution of Fast Radio Bursts

    OpenAIRE

    Oppermann, Niels; Connor, Liam; Pen, Ue-Li

    2016-01-01

    We investigate whether current data on the distribution of observed flux densities of Fast Radio Bursts (FRBs) are consistent with a constant source density in Euclidean space. We use the number of FRBs detected in two surveys with different characteristics along with the observed signal-to-noise ratios of the detected FRBs in a formalism similar to a V/V_max-test to constrain the distribution of flux densities. We find consistency between the data and a Euclidean distribution. Any extension ...

  3. A Euclidean algorithm for integer matrices

    DEFF Research Database (Denmark)

    Lauritzen, Niels; Thomsen, Jesper Funch

    2015-01-01

    We present a Euclidean algorithm for computing a greatest common right divisor of two integer matrices. The algorithm is derived from elementary properties of finitely generated modules over the ring of integers.......We present a Euclidean algorithm for computing a greatest common right divisor of two integer matrices. The algorithm is derived from elementary properties of finitely generated modules over the ring of integers....

  4. A Euclidean bridge to the relativistic constituent quark model

    CERN Document Server

    Hobbs, T J; Miller, Gerald A

    2016-01-01

    ${\\bf Background}$ Knowledge of nucleon structure is today ever more of a precision science, with heightened theoretical and experimental activity expected in coming years. At the same time, a persistent gap lingers between theoretical approaches grounded in Euclidean methods (e.g., lattice QCD, Dyson-Schwinger Equations [DSEs]) as opposed to traditional Minkowski field theories (such as light-front constituent quark models). ${\\bf Purpose}$ Seeking to bridge these complementary worldviews, we explore the potential of a Euclidean constituent quark model (ECQM). This formalism enables us to study the gluonic dressing of the quark-level axial-vector vertex, which we undertake as a test of the framework. ${\\bf Method}$ To access its indispensable elements with a minimum of inessential detail, we develop our ECQM using the simplified quark $+$ scalar diquark picture of the nucleon. We construct a hyperspherical formalism involving polynomial expansions of diquark propagators to marry our ECQM with the results of ...

  5. On the Homogeneous Model Of Euclidean Geometry

    CERN Document Server

    Gunn, Charles

    2011-01-01

    We attach the degenerate signature (n,0,1) to the projectivized dual Grassmann algebra over R(n+1). We explore the use of the resulting Clifford algebra as a model for euclidean geometry. We avoid problems with the degenerate metric by constructing an algebra isomorphism between this Grassmann algebra and its dual, that yields non-metric meet and join operators. We review the Cayley-Klein construction of the projective (homogeneous) model for euclidean geometry leading to the choice of the signature (n,0,1). We focus on the cases of n=2 and n=3 in detail, enumerating the geometric products between simple k- and m-vectors. We establish that versor (sandwich) operators provide all euclidean isometries, both direct and indirect. We locate the spin group, a double cover of the direct euclidean group, inside the even subalgebra of the Clifford algebra, and provide a simple algorithm for calculating the logarithm of such elements. We conclude with an elementary account of euclidean rigid body motion within this fra...

  6. The Euclidean distortion of the lamplighter group

    CERN Document Server

    Austin, Tim; Valette, Alain

    2007-01-01

    We show that the cyclic lamplighter group $C_2 \\bwr C_n$ embeds into Hilbert space with distortion ${\\rm O}(\\sqrt{\\log n})$. This matches the lower bound proved by Lee, Naor and Peres in \\cite{LeeNaoPer}, answering a question posed in that paper. Thus the Euclidean distortion of $C_2 \\bwr C_n$ is $\\Theta(\\sqrt{\\log n})$. Our embedding is constructed explicitly in terms of the irreducible representations of the group. Since the optimal Euclidean embedding of a finite group can always be chosen to be equivariant, as shown by Aharoni, Maurey and Mityagin \\cite{AhaMauMit} and by Gromov (see \\cite{deCTesVal}), such representation-theoretic considerations suggest a general tool for obtaining upper and lower bounds on Euclidean embeddings of finite groups.

  7. Information metric and Euclidean Janus corresponence

    CERN Document Server

    Bak, Dongsu

    2015-01-01

    We consider the quantum information metric of a family of CFTs perturbed by an exactly marginal operator, which has the dual description of the Euclidean Janus geometries. We first review its two dimensional case dual to the three dimensional Janus geometry, which is recently proposed in arXiv:1507.07555. We generalize this correspondence to higher dimensions and get a precise agreement of the both sides. We also propose that the mixed-state information metric of the same family of CFTs is dual to the Euclidean version of the Janus time-dependent black hole geometry.

  8. Information metric and Euclidean Janus correspondence

    Science.gov (United States)

    Bak, Dongsu

    2016-05-01

    We consider the quantum information metric of a family of CFTs perturbed by an exactly marginal operator, which has the dual description of the Euclidean Janus geometries. We first clarify its two dimensional case dual to the three dimensional Janus geometry, which recently appeared in arxiv:arXiv:1507.07555[2]. We generalize this correspondence to higher dimensions and get a precise agreement between the both sides. We also show that the mixed-state information metric of the same family of CFTs has a dual description in the Euclidean version of the Janus time-dependent black hole geometry.

  9. ON SPACELIKE AUSTERE SUBMANIFOLDS IN PSEUDO-EUCLIDEAN SPACE

    Institute of Scientific and Technical Information of China (English)

    Dong Yuxin; Han Yingbo

    2011-01-01

    In this article, we construct some spacelike austere submanifolds in pseduo- Euclidean spaces. We also get some indefinite special Lagrangian submanifolds by con- structing twisted normal bundle of spacelike austere submanifolds in pseduo-Euclidean spaces.

  10. A `Clicked' Tetrameric Hydroxamic Acid Glycopeptidomimetic Antagonizes Sugar-Lectin Interactions On The Cellular Level

    Science.gov (United States)

    Zhang, Hai-Lin; Zang, Yi; Xie, Juan; Li, Jia; Chen, Guo-Rong; He, Xiao-Peng; Tian, He

    2014-07-01

    A tetrameric N-acetyl galactosaminyl (GalNAc) peptidomimetic was constructed by N-acetylation of repeating proline-based hydroxamic acid units, followed by a convergent `click chemistry' coupling. This novel glycopeptidomimetic was determined to effectively antagonize the interaction between a transmembrane hepatic lectin and GalNAc on the cellular level.

  11. Uncertainties of Euclidean Time Extrapolation in Lattice Effective Field Theory

    CERN Document Server

    Lähde, Timo A; Krebs, Hermann; Lee, Dean; Meißner, Ulf-G; Rupak, Gautam

    2014-01-01

    Extrapolations in Euclidean time form a central part of Nuclear Lattice Effective Field Theory (NLEFT) calculations using the Projection Monte Carlo method, as the sign problem in many cases prevents simulations at large Euclidean time. We review the next-to-next-to-leading order NLEFT results for the alpha nuclei up to $^{28}$Si, with emphasis on the Euclidean time extrapolations, their expected accuracy and potential pitfalls. We also discuss possible avenues for improving the reliability of Euclidean time extrapolations in NLEFT.

  12. A linear-time algorithm for Euclidean feature transform sets

    NARCIS (Netherlands)

    Hesselink, Wim H.

    2007-01-01

    The Euclidean distance transform of a binary image is the function that assigns to every pixel the Euclidean distance to the background. The Euclidean feature transform is the function that assigns to every pixel the set of background pixels with this distance. We present an algorithm to compute the

  13. Quantum Biology at the Cellular Level - elements of the research program

    OpenAIRE

    Bordonaro, Michael; Ogryzko, Vasily

    2013-01-01

    Quantum Biology is emerging as a new field at the intersection between fundamental physics and biology, promising novel insights into the nature and origin of biological order. We discuss several elements of QBCL (Quantum Biology at Cellular Level), a research program designed to extend the reach of quantum concepts to higher than molecular levels of biological organization. Key words. decoherence, macroscopic superpositions, basis-dependence, formal superposition, non-classical correlations,...

  14. Cellular burdens and biological effects on tissue level caused by inhaled radon progenies

    CERN Document Server

    Madas, Balázs G; Farkas, Árpád; Szőke, István

    2014-01-01

    In the case of radon exposure, the spatial distribution of deposited radioactive particles is highly inhomogeneous in the central airways. The objective of this research is to investigate the consequences of this heterogeneity regarding cellular burdens in the bronchial epithelium and to study the possible biological effects on tissue level. Applying a computational fluid dynamics program, the deposition distribution of inhaled radon daughters has been determined in a bronchial airway model for 23 minutes of work in the New Mexico uranium mine corresponding to 0.0129 WLM exposure. A numerical epithelium model based on experimental data has been utilized in order to quantify cellular hits and doses. Finally, a carcinogenesis model considering cell death induced cell cycle shortening has been applied to assess the biological responses. Computations present, that cellular dose may reach 1.5 Gy, which is several orders of magnitude higher than tissue dose. The results are in agreement with the histological findin...

  15. Physiological enzymology: The next frontier in understanding protein structure and function at the cellular level.

    Science.gov (United States)

    Lee, Irene; Berdis, Anthony J

    2016-01-01

    Historically, the study of proteins has relied heavily on characterizing the activity of a single purified protein isolated from other cellular components. This classic approach allowed scientists to unambiguously define the intrinsic kinetic and chemical properties of that protein. The ultimate hope was to extrapolate this information toward understanding how the enzyme or receptor behaves within its native cellular context. These types of detailed in vitro analyses were necessary to reduce the innate complexities of measuring the singular activity and biochemical properties of a specific enzyme without interference from other enzymes and potential competing substrates. However, recent developments in fields encompassing cell biology, molecular imaging, and chemical biology now provide the unique chemical tools and instrumentation to study protein structure, function, and regulation in their native cellular environment. These advancements provide the foundation for a new field, coined physiological enzymology, which quantifies the function and regulation of enzymes and proteins at the cellular level. In this Special Edition, we explore the area of Physiological Enzymology and Protein Function through a series of review articles that focus on the tools and techniques used to measure the cellular activity of proteins inside living cells. This article is part of a Special Issue entitled: Physiological Enzymology and Protein Functions. PMID:26277093

  16. Physiological enzymology: The next frontier in understanding protein structure and function at the cellular level.

    Science.gov (United States)

    Lee, Irene; Berdis, Anthony J

    2016-01-01

    Historically, the study of proteins has relied heavily on characterizing the activity of a single purified protein isolated from other cellular components. This classic approach allowed scientists to unambiguously define the intrinsic kinetic and chemical properties of that protein. The ultimate hope was to extrapolate this information toward understanding how the enzyme or receptor behaves within its native cellular context. These types of detailed in vitro analyses were necessary to reduce the innate complexities of measuring the singular activity and biochemical properties of a specific enzyme without interference from other enzymes and potential competing substrates. However, recent developments in fields encompassing cell biology, molecular imaging, and chemical biology now provide the unique chemical tools and instrumentation to study protein structure, function, and regulation in their native cellular environment. These advancements provide the foundation for a new field, coined physiological enzymology, which quantifies the function and regulation of enzymes and proteins at the cellular level. In this Special Edition, we explore the area of Physiological Enzymology and Protein Function through a series of review articles that focus on the tools and techniques used to measure the cellular activity of proteins inside living cells. This article is part of a Special Issue entitled: Physiological Enzymology and Protein Functions.

  17. Euclidean wormholes with minimally coupled scalar fields

    International Nuclear Information System (INIS)

    A detailed study of quantum and semiclassical Euclidean wormholes for Einstein's theory with a minimally coupled scalar field has been performed for a class of potentials. Massless, constant, massive (quadratic in the scalar field) and inverse (linear) potentials admit the Hawking and Page wormhole boundary condition both in the classically forbidden and allowed regions. An inverse quartic potential has been found to exhibit a semiclassical wormhole configuration. Classical wormholes under a suitable back-reaction leading to a finite radius of the throat, where the strong energy condition is satisfied, have been found for the zero, constant, quadratic and exponential potentials. Treating such classical Euclidean wormholes as an initial condition, a late stage of cosmological evolution has been found to remain unaltered from standard Friedmann cosmology, except for the constant potential which under the back-reaction produces a term like a negative cosmological constant. (paper)

  18. The Fourier Transform on Quantum Euclidean Space

    Directory of Open Access Journals (Sweden)

    Kevin Coulembier

    2011-05-01

    Full Text Available We study Fourier theory on quantum Euclidean space. A modified version of the general definition of the Fourier transform on a quantum space is used and its inverse is constructed. The Fourier transforms can be defined by their Bochner's relations and a new type of q-Hankel transforms using the first and second q-Bessel functions. The behavior of the Fourier transforms with respect to partial derivatives and multiplication with variables is studied. The Fourier transform acts between the two representation spaces for the harmonic oscillator on quantum Euclidean space. By using this property it is possible to define a Fourier transform on the entire Hilbert space of the harmonic oscillator, which is its own inverse and satisfies the Parseval theorem.

  19. The Euclidean distribution of fast radio bursts

    Science.gov (United States)

    Oppermann, Niels; Connor, Liam D.; Pen, Ue-Li

    2016-09-01

    We investigate whether current data on the distribution of observed flux densities of fast radio bursts (FRBs) are consistent with a constant source density in Euclidean space. We use the number of FRBs detected in two surveys with different characteristics along with the observed signal-to-noise ratios of the detected FRBs in a formalism similar to a V/Vmax-test to constrain the distribution of flux densities. We find consistency between the data and a Euclidean distribution. Any extension of this model is therefore not data-driven and needs to be motivated separately. As a byproduct we also obtain new improved limits for the FRB rate at 1.4 GHz, which had not been constrained in this way before.

  20. The Euclidean distribution of Fast Radio Bursts

    CERN Document Server

    Oppermann, Niels; Pen, Ue-Li

    2016-01-01

    We investigate whether current data on the distribution of observed flux densities of Fast Radio Bursts (FRBs) are consistent with a constant source density in Euclidean space. We use the number of FRBs detected in two surveys with different characteristics along with the observed signal-to-noise ratios of the detected FRBs in a formalism similar to a V/V_max-test to constrain the distribution of flux densities. We find consistency between the data and a Euclidean distribution. Any extension of this model is therefore not data-driven and needs to be motivated separately. As a byproduct we also obtain new improved limits for the FRB rate at 1.4 GHz, which had not been constrained in this way before.

  1. Euclidean Approach for Entropy of Black Shells

    CERN Document Server

    S., J Robel Arenas

    2016-01-01

    We introduce the concept of black shell, consisting on a massive thin spherical shell contracting toward its gravitational radius from the point of view of an external observer far from the shell, in order to effectively model the gravitational collapse. Considering complementary description of entanglement entropy of a black shell and according to Gibbons-Hawking Euclidean approach, we calculate the Bekenstein-Hawking entropy retrieving horizon integral and discarding boundary at infinity.

  2. On the sensitivity of a Euclidean projection

    Science.gov (United States)

    Izmailov, A. F.; Kurennoy, A. S.

    2014-03-01

    The structure and behavior of Euclidean projections of a point onto a set defined by parametric constraints is studied. Under the Mangasarian-Fromovitz constraint qualification, it is shown that the projection is locally unique and continuous and, if the feasible set is constant, locally Lipschitz continuous as well. Quantitative results are obtained characterizing the asymptotic behavior of projections under perturbations in a given direction.

  3. Simple Euclidean arrangements with one (>=5)-gon

    CERN Document Server

    Leaños, Jesus; Rivera-Martinez, Luis Manuel

    2010-01-01

    Let L be a simple Euclidean arrangement of n pseudolines. It is shown that if L has exactly one (>=5)=gon P, and k is the number of edges of P that are adjacent to an unbounded cell of the subarrangement of L induced by the pseudolines in P, then L has exactly n-k triangles and k+n(n-5)/2 quadrilaterals. We also prove that if each pseudoline of L is adjacent to P then L is stretchable.

  4. NAD+-dependent deacetylase Hst1p controls biosynthesis and cellular NAD+ levels in Saccharomyces cerevisiae.

    Science.gov (United States)

    Bedalov, Antonio; Hirao, Maki; Posakony, Jeffrey; Nelson, Melisa; Simon, Julian A

    2003-10-01

    Nicotine adenine dinucleotide (NAD(+)) performs key roles in electron transport reactions, as a substrate for poly(ADP-ribose) polymerase and NAD(+)-dependent protein deacetylases. In the latter two processes, NAD(+) is consumed and converted to ADP-ribose and nicotinamide. NAD(+) levels can be maintained by regeneration of NAD(+) from nicotinamide via a salvage pathway or by de novo synthesis of NAD(+) from tryptophan. Both pathways are conserved from yeast to humans. We describe a critical role of the NAD(+)-dependent deacetylase Hst1p as a sensor of NAD(+) levels and regulator of NAD(+) biosynthesis. Using transcript arrays, we show that low NAD(+) states specifically induce the de novo NAD(+) biosynthesis genes while the genes in the salvage pathway remain unaffected. The NAD(+)-dependent deacetylase activity of Hst1p represses de novo NAD(+) biosynthesis genes in the absence of new protein synthesis, suggesting a direct effect. The known Hst1p binding partner, Sum1p, is present at promoters of highly inducible NAD(+) biosynthesis genes. The removal of HST1-mediated repression of the NAD(+) de novo biosynthesis pathway leads to increased cellular NAD(+) levels. Transcript array analysis shows that reduction in cellular NAD(+) levels preferentially affects Hst1p-regulated genes in comparison to genes regulated with other NAD(+)-dependent deacetylases (Sir2p, Hst2p, Hst3p, and Hst4p). In vitro experiments demonstrate that Hst1p has relatively low affinity toward NAD(+) in comparison to other NAD(+)-dependent enzymes. These findings suggest that Hst1p serves as a cellular NAD(+) sensor that monitors and regulates cellular NAD(+) levels. PMID:12972620

  5. Resistance to Bacterial Leaf Blight in a Somaclonal Rice Mutant HX-3 at Cellular Level

    Institute of Scientific and Technical Information of China (English)

    GAO Dong-ying; ZHOU Yi-hong; HUANG Xue-qing; SUN Li-hua; LIU Ai-min

    2004-01-01

    The interaction between rice host and its pathogen Xanthomonas oryzae pv. oryzae (Xoo) at cellular level was studied by using a resistant somaclonal mutant HX-3 and its susceptable donor Minghui 63. After inoculation with Xoo strain Zhe 173 (Chinese pathotype Ⅳ), the activity of superoxide dismutase (SOD) and peroxidase (POD) in the callus of Minghui 63 was increased dramatically, and the active oxygen(O2 - .) was produced at a higher rate; Meanwhile, the callus grew slowly with the reduction of protein content. Compared to the activity of SOD and POD, the production rate of O2-. and the fresh weight in HX-3 callus varied little after the inoculation. It could be proposed that there were great differences between the resistance of HX-3 and Mighui 63 at cellular level. There was no difference detected concerning resistance to bacterial leaf blight in HX-3 between the plant and the callus.

  6. Fractal and Euclidean geometric diagnostic methodology of uterine cervix cells = Metodología diagnóstica geométrica fractal y euclidiana de células del cuello uterino

    Directory of Open Access Journals (Sweden)

    Rodríguez Velásquez, Javier Oswaldo

    2014-01-01

    Full Text Available Background: Pathological interpretation of cellular form in cervical cytology is very important for preven- tion of cervical cancer. The methods most frequently used for assessment of this test have reproducibility and inter-observer variability problems. Objective: To make fractal and Euclidean measure- ments to mathematically diagnose normal and pre- malignant cells of cervical squamous epithelium. Methodology: 21 cells with normal, ASCUS or LSIL diagnosis according to the Bethesda system were assessed. Fractal and Euclidean geometric measures of three mathematical objects were calculated: cyto- plasm, nucleus and whole cell. Mathematical propor- tions between these measurements were calculated in order to compare them with conventional classification methods. Results: It was found that the nuclear border measures calculated with the 2-pixel grill and the surface measures could mathematically and objectively differentiate normal cells from the pre-malignant ones (ASCUS and LSIL. Conclusions: An objective and reproducible diagnos- tic method was developed; it allows to identify the evolution towards malignant cellular states based on simultaneous fractal and Euclidean measures, estab- lishing the severity level of ASCUS and LSIL cells.

  7. Foundation of Euclidean and non-Euclidean geometries according to F. Klein

    CERN Document Server

    Redei, L; Stark, M

    1968-01-01

    Foundation of Euclidean and Non-Euclidean Geometries according to F. Klein aims to remedy the deficiency in geometry so that the ideas of F. Klein obtain the place they merit in the literature of mathematics.This book discusses the axioms of betweenness, lattice of linear subspaces, generalization of the notion of space, and coplanar Desargues configurations. The central collineations of the plane, fundamental theorem of projective geometry, and lines perpendicular to a proper plane are also elaborated. This text likewise covers the axioms of motion, basic projective configurations, properties

  8. Regulatory nexus of synthesis and degradation deciphers cellular Nrf2 expression levels.

    Science.gov (United States)

    Suzuki, Takafumi; Shibata, Tatsuhiro; Takaya, Kai; Shiraishi, Kouya; Kohno, Takashi; Kunitoh, Hideo; Tsuta, Koji; Furuta, Koh; Goto, Koichi; Hosoda, Fumie; Sakamoto, Hiromi; Motohashi, Hozumi; Yamamoto, Masayuki

    2013-06-01

    Transcription factor Nrf2 (NF-E2-related factor 2) is essential for oxidative and electrophilic stress responses. While it has been well characterized that Nrf2 activity is tightly regulated at the protein level through proteasomal degradation via Keap1 (Kelch-like ECH-associated protein 1)-mediated ubiquitination, not much attention has been paid to the supply side of Nrf2, especially regulation of Nrf2 gene transcription. Here we report that manipulation of Nrf2 transcription is effective in changing the final Nrf2 protein level and activity of cellular defense against oxidative stress even in the presence of Keap1 and under efficient Nrf2 degradation, determined using genetically engineered mouse models. In excellent agreement with this finding, we found that minor A/A homozygotes of a single nucleotide polymorphism (SNP) in the human NRF2 upstream promoter region (rs6721961) exhibited significantly diminished NRF2 gene expression and, consequently, an increased risk of lung cancer, especially those who had ever smoked. Our results support the notion that in addition to control over proteasomal degradation and derepression from degradation/repression, the transcriptional level of the Nrf2 gene acts as another important regulatory point to define cellular Nrf2 levels. These results thus verify the critical importance of human SNPs that influence the levels of transcription of the NRF2 gene for future personalized medicine. PMID:23572560

  9. Exploring Concepts of Geometry not Euclidean

    Directory of Open Access Journals (Sweden)

    Luiz Ambrozi

    2016-02-01

    Full Text Available With this article we intend to propose different situations of teaching and learning, how they can be applied in schools, mediated by the use of concrete materials and Geogebra software, emphasizing the use of technology in the classroom, that this proposal has the role of assisting in the conceptualization and identification of elements of non-Euclidean geometry. In addition, this short course is designed to be a time of current and continuing education for teachers, with activities to be developed with dynamic geometry and based on the theory of Conceptual Fields of Vergnaud.

  10. Information metric and Euclidean Janus correspondence

    OpenAIRE

    Dongsu Bak

    2016-01-01

    We consider the quantum information metric of a family of CFTs perturbed by an exactly marginal operator, which has the dual description of the Euclidean Janus geometries. We first clarify its two dimensional case dual to the three dimensional Janus geometry, which recently appeared in arXiv:1507.07555 [2] . We generalize this correspondence to higher dimensions and get a precise agreement between the both sides. We also show that the mixed-state information metric of the same family of CFTs ...

  11. Euclidean Quantum Mechanics and Universal Nonlinear Filtering

    CERN Document Server

    Balaji, Bhashyam

    2008-01-01

    An important problem in applied science is the continuous nonlinear filtering problem, i.e., the estimation of a Langevin state that is observed indirectly. In this paper, it is shown that Euclidean quantum mechanics is closely related to the continuous nonlinear filtering problem. The key is the configuration space Feynman path integral representation of the fundamental solution of a Fokker-Planck type of equation termed the Yau Equation of continuous-continuous filtering. A corollary is the equivalence between nonlinear filtering problem and a time-varying Schr\\"odinger equation.

  12. Euclidean Quantum Mechanics and Universal Nonlinear Filtering

    Directory of Open Access Journals (Sweden)

    Bhashyam Balaji

    2009-02-01

    Full Text Available An important problem in applied science is the continuous nonlinear filtering problem, i.e., the estimation of a Langevin state that is observed indirectly. In this paper, it is shown that Euclidean quantum mechanics is closely related to the continuous nonlinear filtering problem. The key is the configuration space Feynman path integral representation of the fundamental solution of a Fokker-Planck type of equation termed the Yau Equation of continuous-continuous filtering. A corollary is the equivalence between nonlinear filtering problem and a time-varying Schr¨odinger equation.

  13. Bochner-Riesz means on Euclidean spaces

    CERN Document Server

    Lu, Shanzhen

    2013-01-01

    This book mainly deals with the Bochner-Riesz means of multiple Fourier integral and series on Euclidean spaces. It aims to give a systematical introduction to the fundamental theories of the Bochner-Riesz means and important achievements attained in the last 50 years. For the Bochner-Riesz means of multiple Fourier integral, it includes the Fefferman theorem which negates the Disc multiplier conjecture, the famous Carleson-Sjölin theorem, and Carbery-Rubio de Francia-Vega's work on almost everywhere convergence of the Bochner-Riesz means below the critical index. For the Bochner-Riesz means o

  14. Non-Euclidean Geometry and Gravitation

    Directory of Open Access Journals (Sweden)

    Stavroulakis N.

    2006-04-01

    Full Text Available A great deal of misunderstandings and mathematical errors are involved in the currently accepted theory of the gravitational field generated by an isotropic spherical mass. The purpose of the present paper is to provide a short account of the rigorous mathematical theory and exhibit a new formulation of the problem. The solution of the corresponding equations of gravitation points out several new and unusual features of the stationary gravitational field which are related to the non-Euclidean structure of the space. Moreover it precludes the black hole from being a mathematical and physical notion.

  15. Within-host spatiotemporal dynamics of plant virus infection at the cellular level.

    Directory of Open Access Journals (Sweden)

    Nicolas Tromas

    2014-02-01

    Full Text Available A multicellular organism is not a monolayer of cells in a flask; it is a complex, spatially structured environment, offering both challenges and opportunities for viruses to thrive. Whereas virus infection dynamics at the host and within-cell levels have been documented, the intermediate between-cell level remains poorly understood. Here, we used flow cytometry to measure the infection status of thousands of individual cells in virus-infected plants. This approach allowed us to determine accurately the number of cells infected by two virus variants in the same host, over space and time as the virus colonizes the host. We found a low overall frequency of cellular infection (<0.3, and few cells were coinfected by both virus variants (<0.1. We then estimated the cellular contagion rate (R, the number of secondary infections per infected cell per day. R ranged from 2.43 to values not significantly different from zero, and generally decreased over time. Estimates of the cellular multiplicity of infection (MOI, the number of virions infecting a cell, were low (<1.5. Variance of virus-genotype frequencies increased strongly from leaf to cell levels, in agreement with a low MOI. Finally, there were leaf-dependent differences in the ease with which a leaf could be colonized, and the number of virions effectively colonizing a leaf. The modeling of infection patterns suggests that the aggregation of virus-infected cells plays a key role in limiting spread; matching the observation that cell-to-cell movement of plant viruses can result in patches of infection. Our results show that virus expansion at the between-cell level is restricted, probably due to the host environment and virus infection itself.

  16. High content analysis at single cell level identifies different cellular responses dependent on nanomaterial concentrations

    Science.gov (United States)

    Manshian, Bella B.; Munck, Sebastian; Agostinis, Patrizia; Himmelreich, Uwe; Soenen, Stefaan J.

    2015-09-01

    A mechanistic understanding of nanomaterial (NM) interaction with biological environments is pivotal for the safe transition from basic science to applied nanomedicine. NM exposure results in varying levels of internalized NM in different neighboring cells, due to variances in cell size, cell cycle phase and NM agglomeration. Using high-content analysis, we investigated the cytotoxic effects of fluorescent quantum dots on cultured cells, where all effects were correlated with the concentration of NMs at the single cell level. Upon binning the single cell data into different categories related to NM concentration, this study demonstrates, for the first time, that quantum dots activate both cytoprotective and cytotoxic mechanisms, resulting in a zero net result on the overall cell population, yet with significant effects in cells with higher cellular NM levels. Our results suggest that future NM cytotoxicity studies should correlate NM toxicity with cellular NM numbers on the single cell level, as conflicting mechanisms in particular cell subpopulations are commonly overlooked using classical toxicological methods.

  17. Comparison of cellular responses induced by low level light in different cell types

    Science.gov (United States)

    Huang, Ying-Ying; Chen, Aaron C.-H.; Sharma, Sulbha K.; Wu, Qiuhe; Hamblin, Michael R.

    2010-02-01

    Discoveries are rapidly being made in multiple laboratories that shed "light" on the fundamental molecular and cellular mechanisms underlying the use of low level light therapy (LLLT) in vitro, in animal models and in clinical practice. Increases in cellular levels of respiration, in cytochrome c oxidase activity, in ATP levels and in cyclic AMP have been found. Increased expression of reactive oxygen species and release of nitric oxide have also been shown. In order for these molecular changes to have a major effect on cell behavior, it is likely that various transcription factors will be activated, possibly via different signal transduction pathways. In this report we compare and contrast the effects of LLLT in vitro on murine embryonic fibroblasts, primary cortical neurons, cardiomyocytes and bone-marrow derived dendritic cells. We also examined two human cell lines, HeLa cancer cells and HaCaT keratinocytes. The effects of 810-nm near-infra-red light delivered at low and high fluences were addressed. Reactive oxygen species generation, transcription factor activation and ATP increases are reported. The data has led to the hypothesis that cells with a high level of mitochondrial activity (mitochondrial membrane potential) have a higher response to light than cells with low mitochondrial activity.

  18. Statistical mechanics, gravity, and Euclidean theory

    International Nuclear Information System (INIS)

    A review of computations of free energy for Gibbs states on stationary but not static gravitational and gauge backgrounds is given. On these backgrounds wave equations for free fields are reduced to eigenvalue problems which depend non-linearly on the spectral parameter. We present a method to deal with such problems. In particular, we demonstrate how some results of the spectral theory of second-order elliptic operators, such as heat kernel asymptotics, can be extended to a class of non-linear spectral problems. The method is used to trace down the relation between the canonical definition of the free energy based on summation over the modes and the covariant definition given in Euclidean quantum gravity. As an application, high-temperature asymptotics of the free energy and of the thermal part of the stress-energy tensor in the presence of rotation are derived. We also discuss statistical mechanics in the presence of Killing horizons where canonical and Euclidean theories are related in a non-trivial way

  19. Quantum Computing in Non Euclidean Geometry

    CERN Document Server

    Resconi, Germano

    2009-01-01

    The recent debate on hyper-computation has raised new questions both on the computational abilities of quantum systems and the Church-Turing Thesis role in Physics. We propose here the idea of geometry of effective physical process as the essentially physical notion of computation. In Quantum mechanics we cannot use the traditional Euclidean geometry but we introduce more sophisticate non Euclidean geometry which include a new kind of information diffuse in the entire universe and that we can represent as Fisher information or active information. We remark that from the Fisher information we can obtain the Bohm and Hiley quantum potential and the classical Schrodinger equation. We can see the quantum phenomena do not affect a limited region of the space but is reflected in a change of the geometry of all the universe. In conclusion any local physical change or physical process is reflected in all the universe by the change of its geometry, This is the deepest meaning of the entanglement in Quantum mechanics a...

  20. Uniform Page Migration Problem in Euclidean Space

    Directory of Open Access Journals (Sweden)

    Amanj Khorramian

    2016-08-01

    Full Text Available The page migration problem in Euclidean space is revisited. In this problem, online requests occur at any location to access a single page located at a server. Every request must be served, and the server has the choice to migrate from its current location to a new location in space. Each service costs the Euclidean distance between the server and request. A migration costs the distance between the former and the new server location, multiplied by the page size. We study the problem in the uniform model, in which the page has size D = 1 . All request locations are not known in advance; however, they are sequentially presented in an online fashion. We design a 2.75 -competitive online algorithm that improves the current best upper bound for the problem with the unit page size. We also provide a lower bound of 2.732 for our algorithm. It was already known that 2.5 is a lower bound for this problem.

  1. Metabolically active portion of fat-free mass: a cellular body composition level modeling analysis

    OpenAIRE

    Wang, ZiMian; Heshka, Stanley; Wang, Jack; Gallagher, Dympna; Deurenberg, Paul; Chen, Zhao; Heymsfield, Steven B

    2006-01-01

    The proportion of fat-free mass (FFM) as body cell mass (BCM) is highly related to whole body resting energy expenditure. However, the magnitude of BCM/FFM may have been underestimated in previous studies. This is because Moore’s equation [BCM (kg) =0.00833 × total body potassium (in mmol)], which was used to predict BCM, underestimates BCM by ~ %. The aims of the present study were to develop a theoretical BCM/FFM model at the cellular level and to explore the influences of sex, age, and adi...

  2. Pengenalan Retina Menggunakan Alihragam Gelombang Singkat dengan Pengukuran Jarak Euclidean Ternormalisasi

    Directory of Open Access Journals (Sweden)

    Aris Wijayanti

    2016-01-01

    Full Text Available Identification of a retinal biometric identification methods with low error rate due to the unique patterns in the retina of blood vessels behind the retina. These patterns can be used as training data for the recognition system is then used for comparison when the identification is done. This study aims to identify the image of the human eye retina, either the left or right side, using image processing techniques and measuring the normalized Euclidean distance. So far, research on biometric systems, particularly with the object of the eye's retina, the eye is done at the owner from the owner's eyes with diverse backgrounds, such as the Messidor database. In this study created a system that can recognize the retinal image using the transformation Haar short waves by measuring the normalized Euclidean distance. Retinal image will be the initial pretreatment process of changing the original image into a gray image, which is then performed using the Haar wavelet feature extraction to obtain the energy that will be used for the normalization of the Euclidean distance, so that the process of recognition by Euclidean values ​​are compared. Testing is done using eye retinal image database taken from Messidor many as 100 of the 300 images taken at random were then stored in a database, the database is one of 100 images stored, the database of 100 images stored 2, and 3 as many as 100 images database stored. Of the best database testing should be done as much as 6 levels of decomposition levels. From the test results have identified the recognition accuracy rate of up to 98%. The greatest degree of familiarity is level 1 that is equal to 98%. Followed usage by 80% level 2, level 3 is 59%, level 4 is 47%, level 5 is 45% and the lowest is the last level 6 that is equal to 37%.   Keywords: Retinal image; Database; Euclidean normalized; Haar Wavelet

  3. Chronobiology at the cellular and molecular levels: models and mechanisms for circadian timekeeping.

    Science.gov (United States)

    Edmunds, L N

    1983-12-01

    This review considers cellular chronobiology and examines, at least in a superficial way, several classes of models and mechanisms that have been proposed for circadian rhythmicity and some of the experimental approaches that have appeared to be most productive. After a brief discussion of temporal organization and the metabolic, epigenetic, and circadian time domains, the general properties of circadian rhythms are enumerated. A survey of independent oscillations in isolated organs, tissues, and cells is followed by a review of selected circadian rhythms in eukaryotic microorganisms, with particular emphasis placed on the rhythm of cell division in the algal flagellate Euglena as a model system illustrating temporal differentiation. In the ensuing section, experimental approaches to circadian clock mechanisms are considered. The dissection of the clock by the use of chemical inhibitors is illustrated for the rhythm of bioluminescence in the marine dinoflagellate Gonyaulax and for the rhythm of photosynthetic capacity in the unicellular green alga Acetabularia. Alternatively, genetic analysis of circadian oscillators is considered in the green alga Chlamydomonas and in the bread mold Neurospora, both of which have yielded clock mutants and mutants having biochemical lesions that exhibit altered clock properties. On the basis of the evidence generated by these experimental approaches, several classes of biochemical and molecular models for circadian clocks have been proposed. These include strictly molecular models, feedback loop (network) models, transcriptional (tape-reading) models, and membrane models; some of their key elements and predictions are discussed. Finally, a number of general unsolved problems at the cellular level are briefly mentioned: cell cycle interfaces, the evolution of circadian rhythmicity, the possibility of multiple cellular oscillators, chronopharmacology and chronotherapy, and cell-cycle clocks in development and aging. PMID:6229999

  4. Fat Euclidean Gravity with Small Cosmological Constant

    CERN Document Server

    Sundrum, Raman

    2003-01-01

    The cosmological constant problem is usually considered an inevitable feature of any effective theory capturing well-tested gravitational and matter physics, without regard to the details of short-distance gravitational couplings. In this paper, a subtle effective description avoiding the problem is presented in a first quantized language, consistent with experiments and the Equivalence Principle. First quantization allows a minimal domain of validity to be carved out by cutting on the proper length of particle worldlines. This is facilitated by working in (locally) Euclidean spacetime, although considerations of unitarity are still addressed by analytic continuation from Lorentzian spacetime. The new effective description demonstrates that the cosmological constant problem {\\it is} sensitive to short-distance details of gravity, which can be probed experimentally. ``Fat Gravity'' toy models are presented, illustrating how gravity might shut off at short but testable distances, in a generally covariant manner...

  5. Supersymmetric solutions to Euclidean Romans supergravity

    Science.gov (United States)

    Alday, Luis F.; Fluder, Martin; Gregory, Carolina Matte; Richmond, Paul; Sparks, James

    2016-02-01

    We study Euclidean Romans supergravity in six dimensions with a non-trivial Abelian R-symmetry gauge field. We show that supersymmetric solutions are in one-to-one correspondence with solutions to a set of differential constraints on an SU(2) structure. As an application of our results we (i) show that this structure reduces at a conformal boundary to the five-dimensional rigid supersymmetric geometry previously studied by the authors, (ii) find a general expression for the holographic dual of the VEV of a BPS Wilson loop, matching an exact field theory computation, (iii) construct holographic duals to squashed Sasaki-Einstein backgrounds, again matching to a field theory computation, and (iv) find new analytic solutions.

  6. Eigentensors of the Lichnerowicz operator in Euclidean Schwarzschild metrics

    Energy Technology Data Exchange (ETDEWEB)

    Martinez-Morales, J.L. [Instituto de Matematicas, Universidad Nacional Autonoma de Mexico, A. P. 273, Admon. de correos 3, C. P. 62251 Cuernavaca, Morelos (Mexico)

    2006-09-01

    Properties of the eigentensors of the Lichnerowicz Laplacian for the Euclidean Schwarzschild metric are discussed together with possible applications to the linear stability of higher-dimensional instantons. The main statement of the article is that any eigentensor of the Lichnerowicz operator in a Euclidean (possibly higher-dimensional) Schwarzschild metric is essentially singular at infinity. (Abstract Copyright [2006], Wiley Periodicals, Inc.)

  7. Euclidean solutions in Einstein-Yang-Mills-dilaton theory

    CERN Document Server

    Brihaye, Y

    2006-01-01

    We present arguments for the existence of a new type of solutions of the Euclidean Einstein-Yang-Mills-dilaton theory in $d=4$ dimensions. Possesing nonvanishing nonabelian charges, these nonselfdual configurations have no counterparts on the Lorentzian section. They provide, however, new saddle points in the Euclidean path integral.

  8. Smoothed analysis of partitioning algorithms for Euclidean functionals

    NARCIS (Netherlands)

    Bläser, Markus; Manthey, Bodo; Rao, B.V. Raghavendra; Dehne, F.; Iacono, J.; Sack, J.-R.

    2011-01-01

    Euclidean optimization problems such as TSP and minimum-length matching admit fast partitioning algorithms that compute near-optimal solutions on typical instances. We develop a general framework for the application of smoothed analysis to partitioning algorithms for Euclidean optimization problems.

  9. The relation between Euclidean and Lorentzian 2D quantum gravity

    NARCIS (Netherlands)

    Ambjørn, J.; Correia, J.; Kristjansen, C.; Loll, R.

    2006-01-01

    Starting from 2D Euclidean quantum gravity, we show that one recovers 2D Lorentzian quantum gravity by removing all baby universes. Using a peeling procedure to decompose the discrete, triangulated geometries along a one-dimensional path, we explicitly associate with each Euclidean space-time a (gen

  10. Face Recognition using Segmental Euclidean Distance

    Directory of Open Access Journals (Sweden)

    Farrukh Sayeed

    2011-09-01

    Full Text Available In this paper an attempt has been made to detect the face using the combination of integral image along with the cascade structured classifier which is built using Adaboost learning algorithm. The detected faces are then passed through a filtering process for discarding the non face regions. They are individually split up into five segments consisting of forehead, eyes, nose, mouth and chin. Each segment is considered as a separate image and Eigenface also called principal component analysis (PCA features of each segment is computed. The faces having a slight pose are also aligned for proper segmentation. The test image is also segmented similarly and its PCA features are found. The segmental Euclidean distance classifier is used for matching the test image with the stored one. The success rate comes out to be 88 per cent on the CG(full database created from the databases of California Institute and Georgia Institute. However the performance of this approach on ORL(full database with the same features is only 70 per cent. For the sake of comparison, DCT(full and fuzzy features are tried on CG and ORL databases but using a well known classifier, support vector machine (SVM. Results of recognition rate with DCT features on SVM classifier are increased by 3 per cent over those due to PCA features and Euclidean distance classifier on the CG database. The results of recognition are improved to 96 per cent with fuzzy features on ORL database with SVM.Defence Science Journal, 2011, 61(5, pp.431-442, DOI:http://dx.doi.org/10.14429/dsj.61.1178

  11. Maintenance of cellular ATP level by caloric restriction correlates chronological survival of budding yeast

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Joon-Seok; Lee, Cheol-Koo, E-mail: cklee2005@korea.ac.kr

    2013-09-13

    Highlights: •CR decreases total ROS and mitochondrial superoxide during the chronological aging. •CR does not affect the levels of oxidative damage on protein and DNA. •CR contributes extension of chronological lifespan by maintenance of ATP level -- Abstract: The free radical theory of aging emphasizes cumulative oxidative damage in the genome and intracellular proteins due to reactive oxygen species (ROS), which is a major cause for aging. Caloric restriction (CR) has been known as a representative treatment that prevents aging; however, its mechanism of action remains elusive. Here, we show that CR extends the chronological lifespan (CLS) of budding yeast by maintaining cellular energy levels. CR reduced the generation of total ROS and mitochondrial superoxide; however, CR did not reduce the oxidative damage in proteins and DNA. Subsequently, calorie-restricted yeast had higher mitochondrial membrane potential (MMP), and it sustained consistent ATP levels during the process of chronological aging. Our results suggest that CR extends the survival of the chronologically aged cells by improving the efficiency of energy metabolism for the maintenance of the ATP level rather than reducing the global oxidative damage of proteins and DNA.

  12. Maintenance of cellular ATP level by caloric restriction correlates chronological survival of budding yeast

    International Nuclear Information System (INIS)

    Highlights: •CR decreases total ROS and mitochondrial superoxide during the chronological aging. •CR does not affect the levels of oxidative damage on protein and DNA. •CR contributes extension of chronological lifespan by maintenance of ATP level -- Abstract: The free radical theory of aging emphasizes cumulative oxidative damage in the genome and intracellular proteins due to reactive oxygen species (ROS), which is a major cause for aging. Caloric restriction (CR) has been known as a representative treatment that prevents aging; however, its mechanism of action remains elusive. Here, we show that CR extends the chronological lifespan (CLS) of budding yeast by maintaining cellular energy levels. CR reduced the generation of total ROS and mitochondrial superoxide; however, CR did not reduce the oxidative damage in proteins and DNA. Subsequently, calorie-restricted yeast had higher mitochondrial membrane potential (MMP), and it sustained consistent ATP levels during the process of chronological aging. Our results suggest that CR extends the survival of the chronologically aged cells by improving the efficiency of energy metabolism for the maintenance of the ATP level rather than reducing the global oxidative damage of proteins and DNA

  13. Cellular level nanomanipulation using atomic force microscope aided with superresolution imaging

    Science.gov (United States)

    Chacko, Jenu Varghese; Harke, Benjamin; Canale, Claudio; Diaspro, Alberto

    2014-10-01

    Atomic force microscopes (AFM) provide topographical and mechanical information of the sample with very good axial resolution, but are limited in terms of chemical specificity and operation time-scale. An optical microscope coupled to an AFM can recognize and target an area of interest using specific identification markers like fluorescence tags. A high resolution fluorescence microscope can visualize fluorescence structures or molecules below the classical optical diffraction limit and reach nanometer scale resolution. A stimulated emission depletion (STED) microscopy superresolution (SR) microscope coupled to an AFM is an example in which the AFM tip gains nanoscale manipulation capabilities. The SR targeting and visualization ability help in fast and specific identification of subdiffraction-sized cellular structures and manoeuvring the AFM tip onto the target. We demonstrate how to build a STED AFM and use it for biological nanomanipulation aided with fast visualization. The STED AFM based bionanomanipulation is presented for the first time in this article. This study points to future nanosurgeries performable at single-cell level and a physical targeted manipulation of cellular features as it is currently used in research domains like nanomedicine and nanorobotics.

  14. Wolbachia-mediated resistance to dengue virus infection and death at the cellular level.

    Directory of Open Access Journals (Sweden)

    Francesca D Frentiu

    Full Text Available BACKGROUND: Dengue is currently the most important arthropod-borne viral disease of humans. Recent work has shown dengue virus displays limited replication in its primary vector, the mosquito Aedes aegypti, when the insect harbors the endosymbiotic bacterium Wolbachia pipientis. Wolbachia-mediated inhibition of virus replication may lead to novel methods of arboviral control, yet the functional and cellular mechanisms that underpin it are unknown. METHODOLOGY/PRINCIPAL FINDINGS: Using paired Wolbachia-infected and uninfected Aedes-derived cell lines and dengue virus, we confirm the phenomenon of viral inhibition at the cellular level. Although Wolbachia imposes a fitness cost to cells via reduced proliferation, it also provides a significant degree of protection from virus-induced mortality. The extent of viral inhibition is related to the density of Wolbachia per cell, with highly infected cell lines showing almost complete protection from dengue infection and dramatically reduced virus titers compared to lines not infected with the bacteria. CONCLUSIONS/SIGNIFICANCE: We have shown that cells infected with Wolbachia display inhibition of dengue virus replication, that the extent of inhibition is related to bacterial density and that Wolbachia infection, although costly, will provide a fitness benefit in some circumstances. Our results parallel findings in mosquitoes and flies, indicating that cell line models will provide useful and experimentally tractable models to study the mechanisms underlying Wolbachia-mediated protection from viruses.

  15. A scientific role for Space Station Freedom: Research at the cellular level

    Science.gov (United States)

    Johnson, Terry C.; Brady, John N.

    1993-01-01

    The scientific importance of Space Station Freedom is discussed in light of the valuable information that can be gained in cellular and developmental biology with regard to the microgravity environment on the cellular cytoskeleton, cellular responses to extracellular signal molecules, morphology, events associated with cell division, and cellular physiology. Examples of studies in basic cell biology, as well as their potential importance to concerns for future enabling strategies, are presented.

  16. Modeling of Trophospheric Ozone Concentrations Using Genetically Trained Multi-Level Cellular Neural Networks

    Institute of Scientific and Technical Information of China (English)

    H. Kurtulus OZCAN; Erdem BILGILI; Ulku SAHIN; O. Nuri UCAN; Cuma BAYAT

    2007-01-01

    Tropospheric ozone concentrations, which are an important air pollutant, are modeled by the use of an artificial intelligence structure. Data obtained from air pollution measurement stations in the city of Istanbul are utilized in constituting the model. A supervised algorithm for the evaluation of ozone concentration using a genetically trained multi-level cellular neural network (ML-CNN) is introduced, developed, and applied to real data. A genetic algorithm is used in the optimization of CNN templates. The model results and the actual measurement results are compared and statistically evaluated. It is observed that seasonal changes in ozone concentrations are reflected effectively by the concentrations estimated by the multilevel-CNN model structure, with a correlation value of 0.57 ascertained between actual and model results. It is shown that the multilevel-CNN modeling technique is as satisfactory as other modeling techniques in associating the data in a complex medium in air pollution applications.

  17. Human metapneumovirus inhibits IFN-β signaling by downregulating Jak1 and Tyk2 cellular levels.

    Directory of Open Access Journals (Sweden)

    Junping Ren

    Full Text Available Human metapneumovirus (hMPV, a leading cause of respiratory tract infections in infants, inhibits type I interferon (IFN signaling by an unidentified mechanism. In this study, we showed that infection of airway epithelial cells with hMPV decreased cellular level of Janus tyrosine kinase (Jak1 and tyrosine kinase 2 (Tyk2, due to enhanced proteosomal degradation and reduced gene transcription. In addition, hMPV infection also reduced the surface expression of type I IFN receptor (IFNAR. These inhibitory mechanisms are different from the ones employed by respiratory syncytial virus (RSV, which does not affect Jak1, Tyk2 or IFNAR expression, but degrades downstream signal transducer and activator of transcription proteins 2 (STAT2, although both viruses are pneumoviruses belonging to the Paramyxoviridae family. Our study identifies a novel mechanism by which hMPV inhibits STAT1 and 2 activation, ultimately leading to viral evasion of host IFN responses.

  18. Modeling of trophospheric ozone concentrations using genetically trained multi-level cellular neural networks

    Science.gov (United States)

    Ozcan, H. Kurtulus; Bilgili, Erdem; Sahin, Ulku; Ucan, O. Nuri; Bayat, Cuma

    2007-09-01

    Tropospheric ozone concentrations, which are an important air pollutant, are modeled by the use of an artificial intelligence structure. Data obtained from air pollution measurement stations in the city of Istanbul are utilized in constituting the model. A supervised algorithm for the evaluation of ozone concentration using a genetically trained multi-level cellular neural network (ML-CNN) is introduced, developed, and applied to real data. A genetic algorithm is used in the optimization of CNN templates. The model results and the actual measurement results are compared and statistically evaluated. It is observed that seasonal changes in ozone concentrations are reflected effectively by the concentrations estimated by the multilevel-CNN model structure, with a correlation value of 0.57 ascertained between actual and model results. It is shown that the multilevel-CNN modeling technique is as satisfactory as other modeling techniques in associating the data in a complex medium in air pollution applications.

  19. How the Venus flytrap actively snaps: hydrodynamic measurements at the cellular level

    Science.gov (United States)

    Colombani, Mathieu; Forterre, Yoel; GEP Team

    2012-11-01

    Although they lack muscle, plants have evolved a remarkable range of mechanisms to create rapid motion, from the rapid folding of sensitive plants to seed dispersal. Of these spectacular examples that have long fascinated scientists, the carnivorous plant Venus flytrap, whose leaves snap together in a fraction of second to capture insects, has long been a paradigm for study. Recently, we have shown that this motion involves a snap-buckling instability due to the shell-like geometry of the leaves of the trap. However, the origin of the movement that allows the plant to cross the instability threshold and actively bend remains largely unknown. In this study, we investigate this active motion using a micro-fluidic pressure probe that gives direct hydraulic and mechanical measurements at the cellular level (osmotic pressure, cell membrane permeability, cell wall elasticity). Our results challenge the role of osmotically-driven water flows usually put forward to explain Venus flytrap's active closure.

  20. MECHANISMS OF DAMAGING EFFECT OF MANGENESE IN TOXIC CONCENTRATIONS ON CELLULAR AND SUBCELLULAR LEVELS

    Directory of Open Access Journals (Sweden)

    Goncharenko A. V.

    2012-11-01

    Full Text Available Influence of subtoxic concentration of manganese chloride in dose equal to LD 50 on condition of plasmatic membranes (model: erythrocytes and functional activity of cell power (model: the isolated liver mitochondrion of rats was studied. It was established that manganese chloride in fixed concentration caused authentic augmentation of sorption capacity of erythrocytes towards alcian blue, influenced increasing of their spontaneous haemolysis and activation of peroxide oxidation of lipids. In experiment on the isolated mitochondrion it was proved that manganese chloride caused dissociation of an oxidizing phosphorusling and complete inhibition of respiration in concentrations of 3 and 4,5mM. These dependences testify that subtoxic concentration of manganese can damage the cell energy. Thus, this pilot research indicated damaging effect of manganese on cellular (erythrocytes and subcellular (mitochondrion levels which are realized through external functioning of membrane structures and deprived them from restoration.

  1. Axial level-dependent molecular and cellular mechanisms underlying the genesis of the embryonic neural plate.

    Science.gov (United States)

    Kondoh, Hisato; Takada, Shinji; Takemoto, Tatsuya

    2016-06-01

    The transcription factor gene Sox2, centrally involved in neural primordial regulation, is activated by many enhancers. During the early stages of embryonic development, Sox2 is regulated by the enhancers N2 and N1 in the anterior neural plate (ANP) and posterior neural plate (PNP), respectively. This differential use of the enhancers reflects distinct regulatory mechanisms underlying the genesis of ANP and PNP. The ANP develops directly from the epiblast, triggered by nodal signal inhibition, and via the combined action of TFs SOX2, OTX2, POU3F1, and ZIC2, which promotes the the ANP development and inhibits other cell lineages. In contrast, the PNP is derived from neuromesodermal bipotential axial stem cells that develop into the neural plate when Sox2 is activated by the N1 enhancer, whereas they develop into the paraxial mesoderm when the N1 enhancer is repressed by the action of TBX6. The axial stem cells are maintained by the activity of WNT3a and T (Brachyury). However, at axial levels more anterior to the 8th somites (cervical levels), the development of both the neural plate and somite proceeds in the absence of WNT3a, T, or TBX6. These observations indicate that distinct molecular and cellular mechanisms determine neural plate genesis based on the axial level, and contradict the classical concept of the term "neural induction," which assumes a pan-neural plate mechanism. PMID:27279156

  2. Axial level-dependent molecular and cellular mechanisms underlying the genesis of the embryonic neural plate.

    Science.gov (United States)

    Kondoh, Hisato; Takada, Shinji; Takemoto, Tatsuya

    2016-06-01

    The transcription factor gene Sox2, centrally involved in neural primordial regulation, is activated by many enhancers. During the early stages of embryonic development, Sox2 is regulated by the enhancers N2 and N1 in the anterior neural plate (ANP) and posterior neural plate (PNP), respectively. This differential use of the enhancers reflects distinct regulatory mechanisms underlying the genesis of ANP and PNP. The ANP develops directly from the epiblast, triggered by nodal signal inhibition, and via the combined action of TFs SOX2, OTX2, POU3F1, and ZIC2, which promotes the the ANP development and inhibits other cell lineages. In contrast, the PNP is derived from neuromesodermal bipotential axial stem cells that develop into the neural plate when Sox2 is activated by the N1 enhancer, whereas they develop into the paraxial mesoderm when the N1 enhancer is repressed by the action of TBX6. The axial stem cells are maintained by the activity of WNT3a and T (Brachyury). However, at axial levels more anterior to the 8th somites (cervical levels), the development of both the neural plate and somite proceeds in the absence of WNT3a, T, or TBX6. These observations indicate that distinct molecular and cellular mechanisms determine neural plate genesis based on the axial level, and contradict the classical concept of the term "neural induction," which assumes a pan-neural plate mechanism.

  3. Energy-Efficient Crowdsensing of Human Mobility and Signal Levels in Cellular Networks.

    Science.gov (United States)

    Foremski, Paweł; Gorawski, Michał; Grochla, Krzysztof; Polys, Konrad

    2015-09-02

    The paper presents a practical application of the crowdsensing idea to measure human mobility and signal coverage in cellular networks. Currently, virtually everyone is carrying a mobile phone, which may be used as a sensor to gather research data by measuring, e.g., human mobility and radio signal levels. However, many users are unwilling to participate in crowdsensing experiments. This work begins with the analysis of the barriers for engaging people in crowdsensing. A survey showed that people who agree to participate in crowdsensing expect a minimum impact on their battery lifetime and phone usage habits. To address these requirements, this paper proposes an application for measuring the location and signal strength data based on energy-efficient GPS tracking, which allows one to perform the measurements of human mobility and radio signal levels with minimum energy utilization and without any engagement of the user. The method described combines measurements from the accelerometer with effective management of the GPS to monitor the user mobility with the decrease in battery lifetime by approximately 20%. To show the applicability of the proposed platform, the sample results of signal level distribution and coverage maps gathered for an LTE network and representing human mobility are shown.

  4. Dual transcriptional-translational cascade permits cellular level tuneable expression control.

    Science.gov (United States)

    Morra, Rosa; Shankar, Jayendra; Robinson, Christopher J; Halliwell, Samantha; Butler, Lisa; Upton, Mathew; Hay, Sam; Micklefield, Jason; Dixon, Neil

    2016-02-18

    The ability to induce gene expression in a small molecule dependent manner has led to many applications in target discovery, functional elucidation and bio-production. To date these applications have relied on a limited set of protein-based control mechanisms operating at the level of transcription initiation. The discovery, design and reengineering of riboswitches offer an alternative means by which to control gene expression. Here we report the development and characterization of a novel tunable recombinant expression system, termed RiboTite, which operates at both the transcriptional and translational level. Using standard inducible promoters and orthogonal riboswitches, a multi-layered modular genetic control circuit was developed to control the expression of both bacteriophage T7 RNA polymerase and recombinant gene(s) of interest. The system was benchmarked against a number of commonly used E. coli expression systems, and shows tight basal control, precise analogue tunability of gene expression at the cellular level, dose-dependent regulation of protein production rates over extended growth periods and enhanced cell viability. This novel system expands the number of E. coli expression systems for use in recombinant protein production and represents a major performance enhancement over and above the most widely used expression systems. PMID:26405200

  5. Low levels of graphene and graphene oxide inhibit cellular xenobiotic defense system mediated by efflux transporters.

    Science.gov (United States)

    Liu, Su; Jiang, Wei; Wu, Bing; Yu, Jing; Yu, Haiyan; Zhang, Xu-Xiang; Torres-Duarte, Cristina; Cherr, Gary N

    2016-06-01

    Low levels of graphene and graphene oxide (GO) are considered to be environmentally safe. In this study, we analyzed the potential effects of graphene and GO at relatively low concentrations on cellular xenobiotic defense system mediated by efflux transporters. The results showed that graphene (<0.5 μg/mL) and GO (<20 μg/mL) did not decrease cell viability, generate reactive oxygen species, or disrupt mitochondrial function. However, graphene and GO at the nontoxic concentrations could increase calcein-AM (CAM, an indicator of membrane ATP-binding cassette (ABC) transporter) activity) accumulation, indicating inhibition of ABC transporters' efflux capabilities. This inhibition was observed even at 0.005 μg/mL graphene and 0.05 μg/mL GO, which are 100 times and 400 times lower than their lowest toxic concentration from cytotoxicity experiments, respectively. The inhibition of ABC transporters significantly increased the toxicity of paraquat and arsenic, known substrates of ABC transporters. The inhibition of ABC transporters was found to be based on graphene and GO damaging the plasma membrane structure and fluidity, thus altering functions of transmembrane ABC transporters. This study demonstrates that low levels of graphene and GO are not environmentally safe since they can significantly make cell more susceptible to other xenobiotics, and this chemosensitizing activity should be considered in the risk assessment of graphene and GO. PMID:26554512

  6. Cellular Heterogeneity in the Level of mtDNA Heteroplasmy in Mouse Embryonic Stem Cells

    Directory of Open Access Journals (Sweden)

    Jitesh Neupane

    2015-11-01

    Full Text Available Variation in the level of mtDNA heteroplasmy in adult tissues is commonly seen in patients with a mixture of wild-type and mutant mtDNA. A mixture of different mtDNA variants may influence such variation and cause mtDNA segregation bias. We analyzed cellular heterogeneity in embryonic stem cells (ESCs derived from a polymorphic mouse model containing NZB and BALB mtDNA genotypes. In ESCs, inter-colony heterogeneity varied up to 61%, whereas intra-colony heterogeneity varied up to 100%. Three out of five cell lines displayed nearly homoplasmic BALB and NZB mtDNA haplotypes in differentiated single cells. The proportion of NZB mtDNA genotype increased with progressive passaging (0.39%; p = 0.002. These results demonstrate the bimodal segregation of mtDNA haplotypes, indicating the occurrence of tissues with variable levels of heteroplasmies in individuals with mtDNA mutations. Furthermore, proliferation of one mtDNA genotype over another may pose the risk of accumulating mutant mtDNAs during subsequent cell divisions.

  7. Cellular Heterogeneity in the Level of mtDNA Heteroplasmy in Mouse Embryonic Stem Cells.

    Science.gov (United States)

    Neupane, Jitesh; Ghimire, Sabitri; Vandewoestyne, Mado; Lu, Yuechao; Gerris, Jan; Van Coster, Rudy; Deroo, Tom; Deforce, Dieter; Vansteelandt, Stijn; De Sutter, Petra; Heindryckx, Björn

    2015-11-17

    Variation in the level of mtDNA heteroplasmy in adult tissues is commonly seen in patients with a mixture of wild-type and mutant mtDNA. A mixture of different mtDNA variants may influence such variation and cause mtDNA segregation bias. We analyzed cellular heterogeneity in embryonic stem cells (ESCs) derived from a polymorphic mouse model containing NZB and BALB mtDNA genotypes. In ESCs, inter-colony heterogeneity varied up to 61%, whereas intra-colony heterogeneity varied up to 100%. Three out of five cell lines displayed nearly homoplasmic BALB and NZB mtDNA haplotypes in differentiated single cells. The proportion of NZB mtDNA genotype increased with progressive passaging (0.39%; p = 0.002). These results demonstrate the bimodal segregation of mtDNA haplotypes, indicating the occurrence of tissues with variable levels of heteroplasmies in individuals with mtDNA mutations. Furthermore, proliferation of one mtDNA genotype over another may pose the risk of accumulating mutant mtDNAs during subsequent cell divisions.

  8. Characterization of cellular traction forces at the single-molecule level

    Science.gov (United States)

    Dunn, Alexander

    2013-03-01

    The ability of cells to generate and respond to mechanical cues is an essential aspect of stem cell differentiation, embryonic development, and our senses of touch and hearing. However, our understanding of the roles of mechanical force in cell biology remains in its infancy, due largely to a lack of tools that measure the forces generated by living cells at the molecular scale. Here we describe a new technique termed Molecular Force Microscopy (MFM) that visualizes the forces exerted by single cellular adhesion molecules with nm, pN, and sub-second resolutions. MFM uses novel FRET-based molecular tension sensors that bind to a glass coverslip and present a binding site for integrins, a ubiquitous class of cell adhesion proteins. Cell-generated forces stretch the MFM sensor molecules, resulting in decreased FRET with increasing load that can be imaged at the single-molecule level. Human foreskin fibroblasts adhere to surfaces functionalized with the MFM probes and develop robust focal adhesions. FRET values measured using MFM indicate forces of between 1 and 4 pN per integrin, thus providing the first direct measurement of the tension per integrin molecule necessary to form stable adhesions. The relatively narrow force distribution suggests that mechanical tension is subject to exquisite feedback and control at the molecular level.

  9. Characterizations of Sobolev spaces in Euclidean spaces and Heisenberg groups

    Institute of Scientific and Technical Information of China (English)

    CUI Xiao-yue; LAM Nguyen; LU Guo-zhen

    2013-01-01

    Recently, many new features of Sobolev spaces W k,p ?RN ? were studied in [4-6, 32]. This paper is devoted to giving a brief review of some known characterizations of Sobolev spaces in Euclidean spaces and describing our recent study of new characterizations of Sobolev spaces on both Heisenberg groups and Euclidean spaces obtained in [12] and [13] and outlining their proofs. Our results extend those characterizations of first order Sobolev spaces in [32] to the Heisenberg group setting. Moreover, our theorems also provide diff erent characterizations for the second order Sobolev spaces in Euclidean spaces from those in [4, 5].

  10. The Euclidean resonance and quantum tunneling

    CERN Document Server

    Ivlev, B I

    2002-01-01

    The extremely small probability of tunneling through an almost classical potential barrier may become not small under the action of the specially adapted non-stationary signal which selects the certain particle energy E_R. For particle energies close to this value, the tunneling rate is not small during a finite interval of time and has a very sharp peak at the energy E_R. After entering inside the barrier, the particle emits electromagnetic quanta and exits the barrier with a lower energy. The signal amplitude can be much less compared to the field of the static barrier. This phenomenon can be called the Euclidean resonance since the under-barrier motion occurs in imaginary time. The resonance may stimulate chemical and biochemical reactions in a selective way by adapting the signal to a certain particular chemical bond. The resonance may be used in search of the soft alpha-decay for which a conventional observation is impossible due to an extremely small decay rate.

  11. An Efficient Representation of Euclidean Gravity I

    CERN Document Server

    Lee, Jungjai; Yang, Hyun Seok

    2011-01-01

    We explore how the topology of spacetime fabric is encoded into the local structure of Riemannian metrics using the gauge theory formulation of Euclidean gravity. In part I, we provide a rigorous mathematical foundation to prove that a general Einstein manifold arises as the sum of SU(2)_L Yang-Mills instantons and SU(2)_R anti-instantons where SU(2)_L and SU(2)_R are normal subgroups of the four-dimensional Lorentz group Spin(4) = SU(2)_L x SU(2)_R. Our proof relies only on the general properties in four dimensions: The Lorentz group Spin(4) is isomorphic to SU(2)_L x SU(2)_R and the six-dimensional vector space of two-forms splits canonically into the sum of three-dimensional vector spaces of self-dual and anti-self-dual two-forms. Consolidating these two, it turns out that the splitting of Spin(4) is deeply correlated with the decomposition of two-forms on four-manifold which occupies a central position in the theory of four-manifolds.

  12. Sulforaphane restores cellular glutathione levels and reduces chronic periodontitis neutrophil hyperactivity in vitro.

    Directory of Open Access Journals (Sweden)

    Irundika H K Dias

    Full Text Available The production of high levels of reactive oxygen species by neutrophils is associated with the local and systemic destructive phenotype found in the chronic inflammatory disease periodontitis. In the present study, we investigated the ability of sulforaphane (SFN to restore cellular glutathione levels and reduce the hyperactivity of circulating neutrophils associated with chronic periodontitis. Using differentiated HL60 cells as a neutrophil model, here we show that generation of extracellular O2 (. - by the nicotinamide adenine dinucleotide (NADPH oxidase complex is increased by intracellular glutathione depletion. This may be attributed to the upregulation of thiol regulated acid sphingomyelinase driven lipid raft formation. Intracellular glutathione was also lower in primary neutrophils from periodontitis patients and, consistent with our previous findings, patients neutrophils were hyper-reactive to stimuli. The activity of nuclear factor erythroid-2-related factor 2 (Nrf2, a master regulator of the antioxidant response, is impaired in circulating neutrophils from chronic periodontitis patients. Although patients' neutrophils exhibit a low reduced glutathione (GSH/oxidised glutathione (GSSG ratio and a higher total Nrf2 level, the DNA-binding activity of nuclear Nrf2 remained unchanged relative to healthy controls and had reduced expression of glutamate cysteine ligase catalytic (GCLC, and modifier (GCLM subunit mRNAs, compared to periodontally healthy subjects neutrophils. Pre-treatment with SFN increased expression of GCLC and GCM, improved intracellular GSH/GSSG ratios and reduced agonist-activated extracellular O2 (. - production in both dHL60 and primary neutrophils from patients with periodontitis and controls. These findings suggest that a deficiency in Nrf2-dependent pathways may underpin susceptibility to hyper-reactivity in circulating primary neutrophils during chronic periodontitis.

  13. Postnatal odorant exposure induces peripheral olfactory plasticity at the cellular level.

    Science.gov (United States)

    Cadiou, Hervé; Aoudé, Imad; Tazir, Bassim; Molinas, Adrien; Fenech, Claire; Meunier, Nicolas; Grosmaitre, Xavier

    2014-04-01

    Mammalian olfactory sensory neurons (OSNs) form the primary elements of the olfactory system. Inserted in the olfactory mucosa lining of the nasal cavity, they are exposed to the environment and their lifespan is brief. Several reports say that OSNs are regularly regenerated during the entire life and that odorant environment affects the olfactory epithelium. However, little is known about the impact of the odorant environment on OSNs at the cellular level and more precisely in the context of early postnatal olfactory exposure. Here we exposed MOR23-green fluorescent protein (GFP) and M71-GFP mice to lyral or acetophenone, ligands for MOR23 or M71, respectively. Daily postnatal exposure to lyral induces plasticity in the population of OSNs expressing MOR23. Their density decreases after odorant exposure, whereas the amount of MOR23 mRNA and protein remain stable in the whole epithelium. Meanwhile, quantitative PCR indicates that each MOR23 neuron has higher levels of olfactory receptor transcripts and also expresses more CNGA2 and phosphodiesterase 1C, fundamental olfactory transduction pathway proteins. Transcript levels return to baseline after 4 weeks recovery. Patch-clamp recordings reveal that exposed MOR23 neurons respond to lyral with higher sensitivity and broader dynamic range while the responses' kinetics were faster. These effects are specific to the odorant-receptor pair lyral-MOR23: there was no effect of acetophenone on MOR23 neurons and no effect of acetophenone and lyral on the M71 population. Together, our results clearly demonstrate that OSNs undergo specific anatomical, molecular, and functional adaptation when chronically exposed to odorants in the early stage of life.

  14. Mechanism of Laser/light beam interaction at cellular and tissue level and study of the influential factors for the application of low level laser therapy

    OpenAIRE

    Khalid, Muhammad Zeeshan

    2016-01-01

    After the discovery of laser therapy it was realized it has useful application of wound healing and reduce pain, but due to the poor understanding of the mechanism and dose response this technique remained to be controversial for therapeutic applications. In order to understand the working and effectiveness different experiments were performed to determine the laser beam effect at the cellular and tissue level. This article discusses the mechanism of beam interaction at tissues and cellular l...

  15. Generators of the sister of Euclidean Picard modular group

    OpenAIRE

    Xie, Baohua

    2015-01-01

    The sister of Eisenstein-Picard modular group was described in [8]. In this paper we give a similar definition of the sister of the Euclidean-Picard modular group and find its generators by using a geometric method.

  16. Oxidized phosphatidylcholines in membrane-level cellular signaling: from biophysics to physiology and molecular pathology.

    Science.gov (United States)

    Volinsky, Roman; Kinnunen, Paavo K J

    2013-06-01

    The oxidation of lipids has been shown to impact virtually all cellular processes. The paradigm has been that this involvement is due to interference with the functions of membrane-associated proteins. It is only recently that methodological advances in molecular-level detection and identification have begun to provide insights into oxidative lipid modification and its involvement in cell signaling as well as in major diseases and inflammation. Extensive evidence suggests a correlation between lipid peroxidation and degenerative neurological diseases such as Parkinson's and Alzheimer's, as well as type 2 diabetes and cancer. Despite the obvious relevance of understanding the molecular basis of the above ailments, the exact modes of action of oxidized lipids have remained elusive. In this minireview, we summarize recent findings on the biophysical characteristics of biomembranes following oxidative derivatization of their lipids, and how these altered properties are involved in both physiological processes and major pathological conditions. Lipid-bearing, oxidatively truncated and functionalized acyl chains are known to modify membrane bulk physical properties, such as thermal phase behavior, bilayer thickness, hydration and polarity profiles, as manifest in the altered structural dynamics of lipid bilayers, leading to augmented membrane permeability, fast lipid transbilayer diffusion (flip-flop), loss of lipid asymmetry (scrambling) and phase segregation (the formation of 'rafts'). These changes, together with the generated reactive lipid derivatives, can be further expected to interfere with lipid-protein interactions, influencing metabolic pathways, causing inflammation, the execution phase in apoptosis and initiating pathological processes. PMID:23506295

  17. A High-Precision Micropipette Sensor for Cellular-Level Real-Time Thermal Characterization

    Directory of Open Access Journals (Sweden)

    Wonseok Chang

    2011-09-01

    Full Text Available We report herein development of a novel glass micropipette thermal sensor fabricated in a cost-effective manner, which is capable of measuring steady thermal fluctuation at spatial resolution of ~2 µm with an accuracy of ±0.01 °C. We produced and tested various micrometer-sized sensors, ranging from 2 µm to 30 µm. The sensor comprises unleaded low-melting-point solder alloy (Sn-based as a core metal inside a pulled borosilicate glass pipette and a thin film of nickel coating outside, creating a thermocouple junction at the tip. The sensor was calibrated using a thermally insulated calibration chamber, the temperature of which can be controlled with an accuracy of ±0.01 °C, and the thermoelectric power (Seebeck coefficient of the sensor was recorded from 8.46 to 8.86 µV/°C. We have demonstrated the capability of measuring temperatures at a cellular level by inserting our temperature sensor into the membrane of a live retinal pigment epithelium cell subjected to a laser beam with a focal spot of 6 μm. We measured transient temperature profiles and the maximum temperatures were in the range of 38–55 ± 0.5 °C.

  18. Adiponectin corrects premature cellular senescence and normalizes antimicrobial peptide levels in senescent keratinocytes.

    Science.gov (United States)

    Jin, Taewon; Kim, Min Jeong; Heo, Won Il; Park, Kui Young; Choi, Sun Young; Lee, Mi-Kyung; Hong, Seung-Phil; Kim, Seong-Jin; Im, Myung; Moon, Nam Ju; Seo, Seong Jun

    2016-09-01

    Stress-induced premature senescence or aging causes dysfunction in the human somatic system. Adiponectin (Acrp30) plays a role in functional recovery, especially with adenosine 3',5'-monophosphate (AMP)-activated protein kinase (AMPK) and silent mating type information regulation 2 homolog 1 (SIRT1). Acrp30 stimulation reduced the premature senescence positive ratio induced by hydrogen peroxide (H2O2) and restituted human β-defensin 2 (hBD-2) levels in senescent keratinocytes. Acrp30 recovered AMPK activity in senescent keratinocytes and increased SIRT1 deacetylation activity. As a result, FoxO1 and FoxO3 transcription activity was recovered. Additionally, Acrp30 stimulation suppresses NFκB p65, which induces abnormal expression of hBD-2 induced by H2O2. In the present study, we have shown that Acrp30 reduces premature senescence and recovers cellular function in keratinocytes. These results suggest a role for Acrp30 as an anti-aging agent to improve impaired skin immune barriers. PMID:27349869

  19. Euclidean supersymmetrization of instantons and self-dual monopoles

    International Nuclear Information System (INIS)

    We show that N=1 D=4 Euclidean supersymmetry leads to the complexification of gauge potentials, and that to have a supersymmetric Euclidean D=4 theory which allows for the selfduality of real gauge potentials, the supersymmetry has to be extended to even N, i.e. at least N=2. We also discuss the supersymmetrization of Manton's procedure of using the dimensional reduction D=4 → D=3 to derive self-dual monopoles from instantons. (author)

  20. Mechanism of Laser/light beam interaction at cellular and tissue level and study of the influential factors for the application of low level laser therapy

    CERN Document Server

    Khalid, Muhammad Zeeshan

    2016-01-01

    After the discovery of laser therapy it was realized it has useful application of wound healing and reduce pain, but due to the poor understanding of the mechanism and dose response this technique remained to be controversial for therapeutic applications. In order to understand the working and effectiveness different experiments were performed to determine the laser beam effect at the cellular and tissue level. This article discusses the mechanism of beam interaction at tissues and cellular level with different light sources and dosimetry principles for clinical application of low level laser therapy. Different application techniques and methods currently in use for clinical treatment has also been reviewed.

  1. Combined phase and X-Ray fluorescence imaging at the sub-cellular level

    International Nuclear Information System (INIS)

    This work presents some recent developments in the field of hard X-ray imaging applied to biomedical research. As the discipline is evolving quickly, new questions appear and the list of needs becomes bigger. Some of them are dealt with in this manuscript. It has been shown that the ID22NI beamline of the ESRF can serve as a proper experimental setup to investigate diverse aspects of cellular research. Together with its high spatial resolution, high flux and high energy range the experimental setup provides bigger field of view, is less sensitive to radiation damages (while taking phase contrast images) and suits well chemical analysis with emphasis on endogenous metals (Zn, Fe, Mn) but also with a possibility for exogenous one's like these found in nanoparticles (Au, Pt, Ag) study. Two synchrotron-based imaging techniques, fluorescence and phase contrast imaging were used in this research project. They were correlated with each other on a number of biological cases, from bacteria E.coli to various cells (HEK 293, PC12, MRC5VA, red blood cells). The explorations made in the chapter 5 allowed preparation of more established and detailed analysis, described in the next chapter where both techniques, X-ray fluorescence and phase contrast imaging, were exploited in order to access absolute metal projected mass fraction in a whole cell. The final image presents for the first time true quantitative information at the sub-cellular level, not biased by the cell thickness. Thus for the first time a fluorescence map serves as a complete quantitative image of a cell without any risk of misinterpretation. Once both maps are divided by each other pixel by pixel (fluorescence map divided by the phase map) they present a complete and final result of the metal (Zn in this work) projected mass fraction in ppm of dry weight. For the purpose of this calculation the analysis was extended to calibration (non-biological) samples. Polystyrene spheres of a known diameter and known

  2. Euclidean Geometric Objects in the Clifford Geometric Algebra of {Origin, 3-Space, Infinity}

    OpenAIRE

    Hitzer, Eckhard

    2013-01-01

    This paper concentrates on the homogeneous (conformal) model of Euclidean space (Horosphere) with subspaces that intuitively correspond to Euclidean geometric objects in three dimensions. Mathematical details of the construction and (useful) parametrizations of the 3D Euclidean object models are explicitly demonstrated in order to show how 3D Euclidean information on positions, orientations and radii can be extracted.

  3. Regulation of biofilm formation and cellular buoyancy through modulating intracellular cyclic di-GMP levels in engineered cyanobacteria.

    Science.gov (United States)

    Agostoni, Marco; Waters, Christopher M; Montgomery, Beronda L

    2016-02-01

    The second messenger cyclic dimeric (3'→5') GMP (cyclic di-GMP or c-di-GMP) has been implicated in the transition between motile and sessile lifestyles in bacteria. In this study, we demonstrate that biofilm formation, cellular aggregation or flocculation, and cellular buoyancy are under the control of c-di-GMP in Synechocystis sp. PCC 6803 (Synechocystis) and Fremyella diplosiphon. Synechocystis is a unicellular cyanobacterium and displays lower levels of c-di-GMP; F. diplosiphon is filamentous and displays higher intracellular c-di-GMP levels. We transformed Synechocystis and F. diplosiphon with a plasmid for constitutive expression of genes encoding diguanylate cylase (DGC) and phosphodiesterase (PDE) proteins from Vibrio cholerae or Escherichia coli, respectively. These engineered strains allowed us to modulate intracellular c-di-GMP levels. Biofilm formation and cellular deposition were induced in the DGC-expressing Synechocystis strain which exhibited high intracellular levels of c-di-GMP; whereas strains expressing PDE in Synechocystis and F. diplosiphon to drive low intracellular levels of c-di-GMP exhibited enhanced cellular buoyancy. In addition, the PDE-expressing F. diplosiphon strain showed elevated chlorophyll levels. These results imply roles for coordinating c-di-GMP homeostasis in regulating native cyanobacterial phenotypes. Engineering exogenous DGC or PDE proteins to regulate intracellular c-di-GMP levels represents an effective tool for uncovering cryptic phenotypes or modulating phenotypes in cyanobacteria for practical applications in biotechnology applicable in photobioreactors and in green biotechnologies, such as energy-efficient harvesting of cellular biomass or the treatment of metal-containing wastewaters.

  4. Cellular Levels of Signaling Factors Are Sensed by β-actin Alleles to Modulate Transcriptional Pulse Intensity

    Directory of Open Access Journals (Sweden)

    Alon Kalo

    2015-04-01

    Full Text Available The transcriptional response of β-actin to extra-cellular stimuli is a paradigm for transcription factor complex assembly and regulation. Serum induction leads to a precisely timed pulse of β-actin transcription in the cell population. Actin protein is proposed to be involved in this response, but it is not known whether cellular actin levels affect nuclear β-actin transcription. We perturbed the levels of key signaling factors and examined the effect on the induced transcriptional pulse by following endogenous β-actin alleles in single living cells. Lowering serum response factor (SRF protein levels leads to loss of pulse integrity, whereas reducing actin protein levels reveals positive feedback regulation, resulting in elevated gene activation and a prolonged transcriptional response. Thus, transcriptional pulse fidelity requires regulated amounts of signaling proteins, and perturbations in factor levels eliminate the physiological response, resulting in either tuning down or exaggeration of the transcriptional pulse.

  5. Morphine Produces Immunosuppressive Effects in Non-human Primates at the Proteomic and Cellular Levels

    Energy Technology Data Exchange (ETDEWEB)

    Brown, Joseph N.; Ortiz, Gabriel M.; Angel, Thomas E.; Jacobs, Jon M.; Gritsenko, Marina A.; Chan, Eric Y.; Purdy, David E.; Murnane, Robert D.; Larsen, Kay; Palermo, Robert E.; Shukla, Anil K.; Clauss, Therese RW; Katze, Michael G.; McCune, Joseph M.; Smith, Richard D.

    2012-05-11

    Morphine has long been known to have immunosuppressive properties in vivo, but the molecular and immunologic changes induced by it are incompletely understood. As a prelude to understanding how these changes might interact with lentiviral infection in vivo, animals from two non-human primate (NHP) species [African green monkey (AGMs) and pigtailed macaque (PTs)] were provided morphine and studied using a systems biology approach. Biological specimens were obtained from multiple sources (e.g., lymph node, colon, cerebrospinal fluid (CSF), and peripheral blood) before and after the administration of morphine (titrated up to a maximum dose of 5 mg/kg over a period of 20 days). Cellular immune, plasma cytokine, and proteome changes were measured and morphine-induced changes in these parameters were assessed on an inter-organ, inter-individual, and inter-species basis. In both species, morphine was associated with decreased levels of (Ki-67+) T cell activation but with only minimal changes in overall T cell counts, neutrophil counts, and NK cells counts. While changes in T cell maturation were observed, these varied across the various tissue/fluid compartments studied. Proteomic analysis revealed a morphine-induced suppressive effect in the lymph node, with decreased abundance of protein mediators involved in the functional categories of energy metabolism, signaling, and maintenance of cell structure. These findings have relevance for understanding the impact of heroin addiction and the opioids used to treat addiction as well as on the interplay between opioid abuse and the response to infection with agents such as the human immunodeficiency virus, type 1 (HIV).

  6. A mathematical model of cortical bone remodeling at cellular level under mechanical stimulus

    Institute of Scientific and Technical Information of China (English)

    Qing-Hua Qin; Ya-Nan Wang

    2012-01-01

    A bone cell population dynamics model for cortical bone remodeling under mechanical stimulus is developed in this paper.The external experiments extracted from the literature which have not been used in the creation of the model are used to test the validity of the model.Not only can the model compare reasonably well with these experimental results such as the increase percentage of final values of bone mineral content (BMC) and bone fracture energy (BFE) among different loading schemes (which proves the validity of the model),but also predict the realtime development pattern of BMC and BFE,as well as the dynamics of osteoblasts (OBA),osteoclasts (OCA),nitric oxide (NO) and prostaglandin E2 (PGE2) for each loading scheme,which can hardly be monitored through experiment.In conclusion,the model is the first of its kind that is able to provide an insight into the quantitative mechanism of bone remodeling at cellular level by which bone cells are activated by mechanical stimulus in order to start resorption/formation of bone mass.More importantly,this model has laid a solid foundation based on which future work such as systemic control theory analysis of bone remodeling under mechanical stimulus can be investigated.The to-be identified control mechanism will help to develop effective drugs and combined nonpharmacological therapies to combat bone loss pathologies.Also this deeper understanding of how mechanical forces quantitatively interact with skeletal tissue is essential for the generation of bone tissue for tissue replacement purposes in tissue engineering.

  7. Physical interpretation and geometrical representation of constant curvature surfaces in Euclidean and pseudo-Euclidean spaces

    International Nuclear Information System (INIS)

    The Riemann and Lorentz constant curvature surfaces are investigated from an Euclidean point of view. The four surfaces (constant positive and constant negative curvatures with definite and non-definite fine elements) are represented as surfaces in a Riemannian or in a particular semi-Riemannian flat space and it is shown that the complex and the hyperbolic numbers allow to obtain the same equations for the corresponding Riemann and Lorentz surfaces, respectively. Moreover it is shown that the geodesics on the Lorentz surfaces states, from a physical point of view, a link between curvature and fields. This result is obtained just as a consequence of the space-time geometrical symmetry, without invoking the famous Einstein general relativity postulate

  8. Euclidean distance harmonic method for establishing theoretical MAPK/Erk signaling pathway in treated breast cancer line MCF-7

    Institute of Scientific and Technical Information of China (English)

    ZHANG Hong-xin; LU Ying-hua; ZHANG Jin-ling

    2007-01-01

    Hierarchical clustering algorithms, such as Pearson's correlation, Euclidean distance, Euclidean distance harmonic,Spearman rank correlation, Kendall's tau, and City-block distance, were used to find the best way to establish theoretical MAPK/Erk signaling pathway on the basis of breast cancer line MCF-7 gene expressions. The algorithm consttucts a hierarchy from top to bottom on the basis of a self-organizing tree. It dynamically finds the number of clusters at each level. It was found that only Euclidean distance harmonic is fit for the analysis of the cascade composed from a RAF1 (c-Raf), a MKNK1, a MAPKK (MEK1/2) to MAPK (Erk) in breast cancer line MCF-7. The result is consistent with the biological experimental MAP/Erk signaling pathway, and the theoretical MAPK/Erk signaling pathway on breast cancer line MCF-7 is set up.

  9. The non-Euclidean revolution with an introduction by H.S.M. Coxeter

    CERN Document Server

    Trudeau, Richard J

    2001-01-01

    How unique and definitive is Euclidean geometry in describing the "real" space in which we live? Richard Trudeau confronts the fundamental question of truth and its representation through mathematical models in The Non-Euclidean Revolution. First, the author analyzes geometry in its historical and philosophical setting; second, he examines a revolution every bit as significant as the Copernican revolution in astronomy and the Darwinian revolution in biology; third, on the most speculative level, he questions the possibility of absolute knowledge of the world. Trudeau writes in a lively, entertaining, and highly accessible style. His book provides one of the most stimulating and personal presentations of a struggle with the nature of truth in mathematics and the physical world. A portion of the book won the Pólya Prize, a distinguished award from the Mathematical Association of America.

  10. Charged particle-induced modification of cellular genomic DNA and gene expression level

    International Nuclear Information System (INIS)

    Aim of this study is to understand cellular and molecular nature of cancer cells survived for long term after charged particle therapy. During the period of 1st year, clonogenic sensitivity of various cancer cell lines against charged particles was investigated by two experimental strategies. Firstly, human glioblastoma cell line, Becker, was investigated for the phenotypic changes after long term survival period (3 weeks). Especially, the cells were revealed to be sensitized toward secondary exposure of charged particles in a way of primary dose-dependence. However, this tendency was clearly eliminated when cells were treated by 5-azacytidine, a DNA methylation inhibitor, before the primary exposure. Thus, epigenetic regulations of cellular genomic DNA were supposed to play important roles in the radiation sensitivity changes of the long-term survived cells. In the second approach, mouse cancer cell line analysis in the presence of 5-azacytidine revealed epigenetic heterogeneity of charged particle sensitivity within the cell population. (author)

  11. Inside s-inner product sets and Euclidean designs

    OpenAIRE

    Nozaki, Hiroshi

    2009-01-01

    A finite set X in the Euclidean space is called an s-inner product set if the set of the usual inner products of any two distinct points in X has size s. First, we give a special upper bound for the cardinality of an s-inner product set on concentric spheres. The upper bound coincides with the known lower bound for the size of a Euclidean 2s-design. Secondly, we prove the non-existence of 2- or 3-inner product sets on two concentric spheres attaining the upper bound for any d>1. The efficient...

  12. Combination of Polymer Technology and Carbon Nanotube Array for the Development of an Effective Drug Delivery System at Cellular Level

    Directory of Open Access Journals (Sweden)

    Riggio Cristina

    2009-01-01

    Full Text Available Abstract In this article, a carbon nanotube (CNT array-based system combined with a polymer thin film is proposed as an effective drug release device directly at cellular level. The polymeric film embedded in the CNT array is described and characterized in terms of release kinetics, while in vitro assays on PC12 cell line have been performed in order to assess the efficiency and functionality of the entrapped agent (neural growth factor, NGF. PC12 cell differentiation, following incubation on the CNT array embedding the alginate delivery film, demonstrated the effectiveness of the proposed solution. The achieved results indicate that polymeric technology could be efficiently embedded in CNT array acting as drug delivery system at cellular level. The implication of this study opens several perspectives in particular in the field of neurointerfaces, combining several functions into a single platform.

  13. Ag Nanoparticles (Ag NM300K) in the Terrestrial Environment: Effects at Population and Cellular Level in Folsomia candida (Collembola)

    OpenAIRE

    Luís André Mendes; Maria, Vera L.; Scott-Fordsmand, Janeck J.; Amorim, Mónica J. B.

    2015-01-01

    The effects of nanomaterials have been primarily assessed based on standard ecotoxicity guidelines. However, by adapting alternative measures the information gained could be enhanced considerably, e.g., studies should focus on more mechanistic approaches. Here, the environmental risk posed by the presence of silver nanoparticles (Ag NM300K) in soil was investigated, anchoring population and cellular level effects, i.e., survival, reproduction (28 days) and oxidative stress markers (0, 2, 4, 6...

  14. High-throughput dual-colour precision imaging for brain-wide connectome with cytoarchitectonic landmarks at the cellular level

    OpenAIRE

    Gong, Hui; Xu, Dongli; Yuan, Jing; Li, Xiangning; Guo, Congdi; Peng, Jie; Li, Yuxin; Schwarz, Lindsay A.; Li, Anan; Hu, Bihe; Xiong, Benyi; Sun, Qingtao; Zhang, Yalun; Liu, Jiepeng; Zhong, Qiuyuan

    2016-01-01

    The precise annotation and accurate identification of neural structures are prerequisites for studying mammalian brain function. The orientation of neurons and neural circuits is usually determined by mapping brain images to coarse axial-sampling planar reference atlases. However, individual differences at the cellular level likely lead to position errors and an inability to orient neural projections at single-cell resolution. Here, we present a high-throughput precision imaging method that c...

  15. From geometry to algebra: the Euclidean way with technology

    Science.gov (United States)

    Ferrarello, Daniela; Flavia Mammana, Maria; Pennisi, Mario

    2016-05-01

    In this paper, we present the results of an experimental classroom activity, history-based with a phylogenetic approach, to achieve algebra properties through geometry. In particular, we used Euclidean propositions, processed them by a dynamic geometry system and translate them into algebraic special products.

  16. The toroidal Hausdorff dimension of 2d Euclidean quantum gravity

    DEFF Research Database (Denmark)

    Ambjorn, Jan; Budd, Timothy George

    2013-01-01

    The lengths of shortest non-contractible loops are studied numerically in 2d Euclidean quantum gravity on a torus coupled to conformal field theories with central charge less than one. We find that the distribution of these geodesic lengths displays a scaling in agreement with a Hausdorff dimensi...

  17. A Note on Minimal Surfaces in Euclidean 3-Space

    Institute of Scientific and Technical Information of China (English)

    Zu Huan YU; Qing Zhong LI

    2007-01-01

    In this note, a construction of minimal surfaces in Euclidean 3-space is given. By using the product of Weierstrass data of two known minimal surfaces, one gets a new Weierstrass data and a corresponding minimal surface from the Weierstrass representation.

  18. Weierstrass representations for harmonic morphisms on Euclidean spaces and spheres

    CERN Document Server

    Baird, P

    1995-01-01

    We construct large families of harmonic morphisms which are holomorphic with respect to Hermitian structures by finding heierarchies of Weierstrass-type representations. This enables us to find new examples of complex-valued harmonic morphisms from Euclidean spaces and spheres.

  19. Timed Fast Exact Euclidean Distance (tFEED) maps

    NARCIS (Netherlands)

    Schouten, Theo E.; Kuppens, Harco; Broek, van den Egon L.; Kehtarnavaz, Nasser; Laplante, Philip A.

    2005-01-01

    In image and video analysis, distance maps are frequently used. They provide the (Euclidean) distance (ED) of background pixels to the nearest object pixel. In a naive implementation, each object pixel feeds its (exact) ED to each background pixel; then the minimum of these values denotes the ED to

  20. Smoothed analysis of partitioning algorithms for Euclidean functionals

    NARCIS (Netherlands)

    Bläser, Markus; Manthey, Bodo; Rao, B.V. Raghavendra

    2013-01-01

    Euclidean optimization problems such as TSP and minimum-length matching admit fast partitioning algorithms that compute near-optimal solutions on typical instances. In order to explain this performance, we develop a general framework for the application of smoothed analysis to partitioning algorithm

  1. Convex bodies in Euclidean and Weil-Petersson geometries

    CERN Document Server

    Yamada, Sumio

    2011-01-01

    On a convex body in a Euclidean space, we introduce a new variational formulation for its Funk metric, a Finsler metric compatible with the tautological Finsler structure of the convex body. We generalize the metric on Teichmuller spaces with the Weil-Petersson distance function. A set of similarities the resulting metric structure shares with Thurston's asymmetric metric is noted.

  2. CBFA1 and topoisomerase I mRNA levels decline during cellular aging of human trabecular osteoblasts

    DEFF Research Database (Denmark)

    Christiansen, M; Kveiborg, Marie; Kassem, M;

    2000-01-01

    In order to understand the reasons for age-related impairment of the function of bone forming osteoblasts, we have examined the steady-state mRNA levels of the transcription factor CBFA1 and topoisomerase I during cellular aging of normal human trabecular osteoblasts, by the use of semiquantitative...... reverse transcriptase-polymerase chain reaction (RT-PCR). There is a progressive and significant reduction of the CBFA1 steady-state mRNA level down to 50% during cellular aging of human osteoblasts. In comparison to the normal cells, human osteosarcoma cell lines SaOS-2 and KHOS/NP, and the SV40......-transformed human lung fibroblast cell line MRC5V2 have 20 to 40% higher levels of CBFA1 mRNA. Similar levels of CBFA1 mRNA are detectable in normal human skin fibroblasts, and these cells also exhibit an age-related decline to the same extent. In addition, the expression of topoisomerase I is reduced by 40...

  3. Mechanistic model of radon-induced lung cancer risk at low exposure levels based on cellular alpha particle hits

    Energy Technology Data Exchange (ETDEWEB)

    Werner, Hofmann; Hatim, Fakir [Salzburg Univ., Div. of Physics and Biophysics, Dept. of Material Science (Austria); Lucia-Adina, Truta-Popa [Babes-Bolyai Univ., Faculty of Physics (Romania)

    2006-07-01

    To explore the role of the multiplicity of cellular hits by radon progeny alpha particles for lung cancer incidence, the number of single and multiple alpha particle hits were computed for basal and secretory cells in the bronchial epithelium of human airway bifurcations employing Monte Carlo methods. Hot spots of alpha particle hits were observed at the branching points of bronchial airway bifurcations, suggesting that multiple alpha particle hits may occur primarily at carinal ridges. Random alpha particle intersections of bronchial cells during a given exposure period, selected from a Poisson distribution, were simulated by an initiation-promotion model, based on experimentally observed cellular transformation and survival functions. To consider potential bystander effects, which have been observed in cellular in vitro studies, alpha particle interactions were also simulated for larger sensitive target volumes in bronchial epithelium, consisting of a collection of cells. Lung cancer risk simulations indicated that cancer induction for continuous exposures is related to the cycle time of an irradiated cell, thus exhibiting a distinct dose-rate effect. While the dominant role of single hits leads to a linear dose-response relationship at low radon exposure levels, predicted lung cancer risk for a collection of interacting cells exhibits a linear-quadratic response, suggesting that bystander effects, if operating at all under in vivo irradiations, may be restricted to a small number of adjacent cells. (author)

  4. Relationship between chicken cellular immunity and endotoxin levels in dust from chicken housing environments.

    Science.gov (United States)

    Roque, Katharine; Shin, Kyung-Min; Jo, Ji-Hoon; Kim, Hyoung-Ah; Heo, Yong

    2015-01-01

    Hazardous biochemical agents in animal husbandry indoor environments are known to promote the occurrence of various illnesses among workers and animals. The relationship between endotoxin levels in dust collected from chicken farms and various immunological markers was investigated. Peripheral blood was obtained from 20 broiler chickens and 20 laying hens from four different chicken farms in Korea. Concentrations of total or respirable dust in the inside the chicken farm buildings were measured using a polyvinyl chloride membrane filter and mini volume sampler. Endotoxin levels in the dust were determined by the Limulus Amebocyte Lysate Kinetic method. Interferon-γ production by peripheral blood mononuclear cells stimulated with concanavalin A was significantly lower in broilers or layers from the farms with higher endotoxin concentrations than the chickens from the farms with lower endotoxin levels. An opposite pattern was observed for plasma cortisol concentrations with higher cortisol levels found in chickens from the farms with higher endotoxin levels. When peripheral lymphocytes were examined, the percentage of CD3(-)Ia(+) B cells was lower in layers from farms with higher endotoxin levels than those from locations with lower endotoxin levels. Overall, these results suggest a probable negative association between dust endotoxin levels and cell-mediated immunity in chickens.

  5. Ag Nanoparticles (Ag NM300K) in the Terrestrial Environment: Effects at Population and Cellular Level in Folsomia candida (Collembola).

    Science.gov (United States)

    Mendes, Luís André; Maria, Vera L; Scott-Fordsmand, Janeck J; Amorim, Mónica J B

    2015-10-01

    The effects of nanomaterials have been primarily assessed based on standard ecotoxicity guidelines. However, by adapting alternative measures the information gained could be enhanced considerably, e.g., studies should focus on more mechanistic approaches. Here, the environmental risk posed by the presence of silver nanoparticles (Ag NM300K) in soil was investigated, anchoring population and cellular level effects, i.e., survival, reproduction (28 days) and oxidative stress markers (0, 2, 4, 6, 10 days). The standard species Folsomia candida was used. Measured markers included catalase (CAT), glutathione reductase (GR), glutathione S-transferase (GST), total glutathione (TG), metallothionein (MT) and lipid peroxidation (LPO). Results showed that AgNO₃ was more toxic than AgNPs at the population level: reproduction EC₂₀ and EC₅₀ was ca. 2 and 4 times lower, respectively. At the cellular level Correspondence Analysis showed a clear separation between AgNO₃ and AgNP throughout time. Results showed differences in the mechanisms, indicating a combined effect of released Ag⁺ (MT and GST) and of AgNPs (CAT, GR, TG, LPO). Hence, clear advantages from mechanistic approaches are shown, but also that time is of importance when measuring such responses. PMID:26473892

  6. Ag Nanoparticles (Ag NM300K in the Terrestrial Environment: Effects at Population and Cellular Level in Folsomia candida (Collembola

    Directory of Open Access Journals (Sweden)

    Luís André Mendes

    2015-10-01

    Full Text Available The effects of nanomaterials have been primarily assessed based on standard ecotoxicity guidelines. However, by adapting alternative measures the information gained could be enhanced considerably, e.g., studies should focus on more mechanistic approaches. Here, the environmental risk posed by the presence of silver nanoparticles (Ag NM300K in soil was investigated, anchoring population and cellular level effects, i.e., survival, reproduction (28 days and oxidative stress markers (0, 2, 4, 6, 10 days. The standard species Folsomia candida was used. Measured markers included catalase (CAT, glutathione reductase (GR, glutathione S-transferase (GST, total glutathione (TG, metallothionein (MT and lipid peroxidation (LPO. Results showed that AgNO3 was more toxic than AgNPs at the population level: reproduction EC20 and EC50 was ca. 2 and 4 times lower, respectively. At the cellular level Correspondence Analysis showed a clear separation between AgNO3 and AgNP throughout time. Results showed differences in the mechanisms, indicating a combined effect of released Ag+ (MT and GST and of AgNPs (CAT, GR, TG, LPO. Hence, clear advantages from mechanistic approaches are shown, but also that time is of importance when measuring such responses.

  7. Real-time reporting and internet-accessible cellular based coastal sea level gauge

    Digital Repository Service at National Institute of Oceanography (India)

    Desai, R.G.P.; Joseph, A.; Agarvadekar, Y.; Mehra, P.; Dabholkar, N.; Parab, A.; Gouveia, A.D.; Tengali, S.

    within a cylindrical protective housing, which in turn is rigidly held within the vertical legs and the interconnecting triangular collar of the mechanical structure used for mounting the entire gauge. The gauge and the data communication modem... for acquisition of data from sea-level gauge, from communication overload. These modems need only a few simple commands to upload data on a remote FTP server. Data communication is always initiated by the sea-level gauge. After communication link is established, a...

  8. Effect of electromagnetic fields at 2.45 GHz on the levels of cellular stress proteins HSP-90 and 70 in the rat thyroid

    International Nuclear Information System (INIS)

    In this study we analyzed the cellular stress levels achieved by heat shock proteins (HSP) 90 and 70 in rat thyroid tissue after exposure to radio waves in TWG experimental system. Parallel measurements of body stress in animals by rectal temperature probes allow us to determine whether there is any interaction between temperature increases and cellular stress.

  9. Lorentz connection from a conformal gauge theory of Euclidean space

    CERN Document Server

    Hazboun, Jeffrey S

    2013-01-01

    The quotient of the conformal group of Euclidean 4-space by its Weyl subgroup results in a geometry possessing many of the properties of relativistic phase space, including both a natural symplectic form and non-degenerate Killing metric. When we complete the identification with phase space by requiring the existence of orthogonal, canonically conjugate, metric submanifolds, we find that the induced metric and the spin connection are Lorentzian on the submanifolds, despite the Euclidean starting pont. By examining the structure equations of the biconformal space in an orthonormal frame adapted to its phase space properties, we also find that two new tensor fields emerge from this geometry. The first is a combination of the scale factor on the metric with the Weyl vector. The second comes from the components of the spin connection, symmetric with respect to the new metric. Though this field comes from the spin connection it transforms homogeneously.

  10. Learning to Detect Spam: Naive-Euclidean Approach

    Directory of Open Access Journals (Sweden)

    Tony Y.T. Chan

    2008-12-01

    Full Text Available A method is proposed for learning to classify spam and nonspamemails. It combines the strategy of the Best Stepwise Feature Selection with a classifier of Euclidean nearest-neighbor. Each text email is first transformed into a vector of D-dimensional Euclidean space. Emails were divided into training and test sets in the manner of 10-fold crossvalidation. Three experiments were performed, and their elapsed CPU times and accuracies reported. The proposed spam detection learner was found to be extremely fast in recognition and with good error rates. It could be used as a baseline learning agent, in terms of CPU time and accuracy, against which other learning agents can be measured.

  11. Active biopolymer networks generate scale-free but euclidean clusters

    CERN Document Server

    Sheinman, M; Alvarado, J; Koenderink, G H; MacKintosh, F C

    2014-01-01

    We report analytical and numerical modelling of active elastic networks, motivated by experiments on crosslinked actin networks contracted by myosin motors. Within a broad range of parameters, the motor-driven collapse of active elastic networks leads to a critical state. We show that this state is qualitatively different from that of the random percolation model. Intriguingly, it possesses both euclidean and scale-free structure with Fisher exponent smaller than $2$. Remarkably, an indistinguishable Fisher exponent and the same euclidean structure is obtained at the critical point of the random percolation model after absorbing all enclaves into their surrounding clusters. We propose that in the experiment the enclaves are absorbed due to steric interactions of network elements. We model the network collapse, taking into account the steric interactions. The model shows how the system robustly drives itself towards the critical point of the random percolation model with absorbed enclaves, in agreement with th...

  12. Short-distance analysis for algebraic euclidean field theory

    CERN Document Server

    Schlingemann, D

    1999-01-01

    Recently D. Buchholz and R. Verch have proposed a method for implementing in algebraic quantum field theory ideas from renormalization group analysis of short-distance (high energy) behavior by passing to certain scaling limit theories. Buchholz and Verch distinguish between different types of theories where the limit is unique, degenerate, or classical, and the method allows in principle to extract the `ultraparticle' content of a given model, i.e. to identify particles (like quarks and gluons) that are not visible at finite distances due to `confinement'. It is therefore of great importance for the physical interpretation of the theory. The method has been illustrated in a simple model in with some rather surprising results. This paper will focus on the question how the short distance behavior of models defined by euclidean means is reflected in the corresponding behavior of their Minkowski counterparts. More specifically, we shall prove that if a euclidean theory has some short distance limit, then it is p...

  13. Embedding spacetime via a geodesically equivalent metric of Euclidean signature

    CERN Document Server

    Jonsson, Rickard

    2001-01-01

    Starting from the equations of motion in a 1 + 1 static, diagonal, Lorentzian spacetime, such as the Schwarzschild radial line element, I find another metric, but with Euclidean signature, which produces the same geodesics x(t). This geodesically equivalent, or dual, metric can be embedded in ordinary Euclidean space. On the embedded surface freely falling particles move on the shortest path. Thus one can visualize how acceleration in a gravitational field is explained by particles moving freely in a curved spacetime. Freedom in the dual metric allows us to display, with substantial curvature, even the weak gravity of our Earth. This may provide a nice pedagogical tool for elementary lectures on general relativity. I also study extensions of the dual metric scheme to higher dimensions. In an addendum I extend the analysis concerning the shape of an embedding of the dual spacetime of a line through a planet of constant proper density.

  14. Riemannian means on special euclidean group and unipotent matrices group.

    Science.gov (United States)

    Duan, Xiaomin; Sun, Huafei; Peng, Linyu

    2013-01-01

    Among the noncompact matrix Lie groups, the special Euclidean group and the unipotent matrix group play important roles in both theoretic and applied studies. The Riemannian means of a finite set of the given points on the two matrix groups are investigated, respectively. Based on the left invariant metric on the matrix Lie groups, the geodesic between any two points is gotten. And the sum of the geodesic distances is taken as the cost function, whose minimizer is the Riemannian mean. Moreover, a Riemannian gradient algorithm for computing the Riemannian mean on the special Euclidean group and an iterative formula for that on the unipotent matrix group are proposed, respectively. Finally, several numerical simulations in the 3-dimensional case are given to illustrate our results.

  15. The efficient algorithms for achieving Euclidean distance transformation.

    Science.gov (United States)

    Shih, Frank Y; Wu, Yi-Ta

    2004-08-01

    Euclidean distance transformation (EDT) is used to convert a digital binary image consisting of object (foreground) and nonobject (background) pixels into another image where each pixel has a value of the minimum Euclidean distance from nonobject pixels. In this paper, the improved iterative erosion algorithm is proposed to avoid the redundant calculations in the iterative erosion algorithm. Furthermore, to avoid the iterative operations, the two-scan-based algorithm by a deriving approach is developed for achieving EDT correctly and efficiently in a constant time. Besides, we discover when obstacles appear in the image, many algorithms cannot achieve the correct EDT except our two-scan-based algorithm. Moreover, the two-scan-based algorithm does not require the additional cost of preprocessing or relative-coordinates recording.

  16. Antioxidant factors, nitric oxide levels, and cellular damage in leprosy patients

    Directory of Open Access Journals (Sweden)

    Taysa Ribeiro Schalcher

    2013-09-01

    Full Text Available Introduction The immune response caused by Mycobacterium leprae is a risk factor for the development of oxidative stress (OS in leprosy patients. This study aimed to assess OS in leprosy patients before the use of a multidrug therapy. Methods We evaluated the nitric oxide (NO concentration; antioxidant capacity; levels of malondialdehyde, methemoglobin and reduced glutathione; and the activity of catalase and superoxide dismutase (SOD in leprosy patients. Results We observed lower SOD activity in these leprosy patients; however, the NO levels and antioxidant capacity were increased. Conclusions The infectious process in response to M. leprae could primarily be responsible for the OS observed in these patients.

  17. Change of Measure between Light Travel Time and Euclidean Distances

    Directory of Open Access Journals (Sweden)

    Heymann Y.

    2013-04-01

    Full Text Available The problem of cosmological distances is approached using a method based on the propagation of light in an expanding Universe. From the chan ge of measure between Light Travel Time and Euclidean Distances, a formula is deri ved to compute distances as a function of redshift. This formula is identical to Matti g’s formula (with q 0 = 1 / 2 which is based on Friedmann’s equations of general relativi ty.

  18. General Nth order integrals of motion in the Euclidean plane

    Science.gov (United States)

    Post, S.; Winternitz, P.

    2015-10-01

    The general form of an integral of motion that is a polynomial of order N in the momenta is presented for a Hamiltonian system in two-dimensional Euclidean space. The classical and the quantum cases are treated separately, emphasizing both the similarities and the differences between the two. The main application will be to study Nth order superintegrable systems that allow separation of variables in the Hamilton-Jacobi and Schrödinger equations, respectively.

  19. Special geometry of Euclidean supersymmetry I: Vector multiplets

    Energy Technology Data Exchange (ETDEWEB)

    Cortes, V.; Mayer, C.; Saueressig, F.; Mohaupt, T. E-mail: mohaupt@tpi.uni-jena.de

    2004-02-01

    We construct the general action for Abelian vector multiplets in rigid 4-dimensional Euclidean (instead of Minkowskian) N=2 supersymmetry, i.e., over space-times with a positive definite instead of a Lorentzian metric. The target manifolds for the scalar fields turn out to be para-complex manifolds endowed with a particular kind of special geometry, which we call affine special para-Kahler geometry. We give the precise definition and develop the mathematical theory of such manifolds. The relation to the affine special Kahler manifolds appearing in Minkowskian N=2 supersymmetry is discussed. Starting from the general five-dimensional vector multiplet action we consider dimensional reduction over time and space in parallel, providing a dictionary between the resulting Euclidean and Minkowskian theories. Then we reanalyze supersymmetry in four dimensions and find that any (para-)holomorphic prepotential defines a supersymmetric Lagrangian, provided that we add a specific four-fermion term, which cannot be obtained by dimensional reduction. We show that the Euclidean action and supersymmetry transformations, when written in terms of para-holomorphic coordinates, take exactly the same form as their Minkowskina counterparts. The appearance of a para-complex and complex structure in the Euclidean and Minkowskian theory, respectively, is traced back to properties of the underlying R-symmetry groups. Finally, we indicate how our work will be extended to other types of multiplets and to supergravity in the future and explain the relevance of this project for the study of instantons, solitons and cosmological solutions in supergravity and M-theory. (author)

  20. Defects and boundary layers in non-Euclidean plates

    CERN Document Server

    Gemmer, John

    2012-01-01

    We investigate the behaviour of non-Euclidean plates with constant negative Gaussian curvature using the F\\"oppl-von K\\'arm\\'an reduced theory of elasticity. Motivated by recent experimental results, we focus on annuli with a periodic profile. We prove rigorous upper and lower bounds for the elastic energy that scales like the thickness squared. We also investigate the scaling with thickness of boundary layers where the stretching energy is concentrated with decreasing thickness.

  1. Euclidean Configuration Space Renormalization, Residues and Dilation Anomaly

    CERN Document Server

    Nikolov, Nikolay M; Todorov, Ivan

    2013-01-01

    Configuration (x-)space renormalization of Euclidean Feynman amplitudes in a massless quantum field theory is reduced to the study of local extensions of associate homogeneous distributions. Primitively divergent graphs are renormalized, in particular, by subtracting the residue of an analytically regularized expression. Examples are given of computing residues that involve zeta values. The renormalized Green functions are again associate homogeneous distributions of the same degree that transform under indecomposable representations of the dilation group.

  2. Euclidean Upgrade from a Minimal Number of Segments

    OpenAIRE

    Schilling, Tanja; Pajdla, Tomas

    2013-01-01

    In this paper, we propose an algebraic approach to upgrade a projective reconstruction to a Euclidean one, and aim at computing the rectifying homography from a minimal number of 9 segments of known length. Constraints are derived from these segments which yield a set of polynomial equations that we solve by means of Gr\\"obner bases. We explain how a solver for such a system of equations can be constructed from simplified template data. Moreover, we present experiments that demonstrate that t...

  3. General Nth order integrals of motion in the Euclidean plane

    International Nuclear Information System (INIS)

    The general form of an integral of motion that is a polynomial of order N in the momenta is presented for a Hamiltonian system in two-dimensional Euclidean space. The classical and the quantum cases are treated separately, emphasizing both the similarities and the differences between the two. The main application will be to study Nth order superintegrable systems that allow separation of variables in the Hamilton–Jacobi and Schrödinger equations, respectively. (paper)

  4. Dynamics of Induced Surfaces in Four-Dimensional Euclidean Space

    OpenAIRE

    Bracken, Paul

    2006-01-01

    The Davey Stewartson hierarchy will be developed based on a set of three matrix differential operators. These equations will act as evolution equations for different types of surface deformation in Euclidean four space. The Weierstrass representation for surfaces will be developed and its uniqueness up to gauge transformations will be reviewed. Applications of the hierarchy will be given with regard to generating deformations of surfaces, and it will be shown that the Willmore functional is p...

  5. Quantum Brans-Dicke Gravity in Euclidean Path Integral Formulation

    OpenAIRE

    Kim, Hongsu

    1997-01-01

    The conformal structure of Brans-Dicke gravity action is carefully studied. It is discussed that Brans-Dicke gravity action has definitely no conformal invariance. It is shown, however, that this lack of conformal invariance enables us to demonstrate that Brans-Dicke theory appears to have a better short-distance behavior than Einstein gravity as far as Euclidean path integral formulation for quantum gravity is concerned.

  6. Algorithms for degree-constrained Euclidean Steiner minimal tree

    Institute of Scientific and Technical Information of China (English)

    Zhang Jin; Ma Liang; Zhang Liantang

    2008-01-01

    A new problem of degree-constrained Euclidean Steiner minimal tree is discussed,which is quite useful in several fields.Although it is slightly different from the traditional degree-constrained minimal spanning tree,it is aho NP-hard.Two intelligent algorithms are proposed in an attempt to solve this difficult problem.Series of numerical examples are tested,which demonstrate that the algorithms also work well in practice.

  7. Selected materials of the international workshop on radiation risk and its origin at molecular and cellular level

    International Nuclear Information System (INIS)

    The workshop ''International Workshop on Radiation Risk and its Origin at Molecular and Cellular Level'' was held at The Tokai Research Establishment, Japan Atomic Energy Research Institute, on the 6th and 7th of February 2003. The Laboratory of Radiation Risk Analysis of JAERI organized it. This international workshop attracted scientists from several different scientific areas, including radiation physics, radiation biology, molecular biology, crystallography of biomolecules, modeling and bio-informatics. Several foreign and domestic keynote speakers addresses the very fundamental areas of radiation risk and tried to establish a link between the fundamental studies at the molecular and cellular level and radiation damages at the organism. The symposium consisted of 13 oral lectures, 10 poster presentations and panel discussion. The 108 participants attended the workshop. This publication comprises of proceedings of oral and poster presentations where available. For the rest of contributions the abstracts or/and selections of presentation materials are shown instead. The 5 papers are indexed individually. (J.P.N.)

  8. Statistical assessment of trophic conditions: squared Euclidean distance approach

    Directory of Open Access Journals (Sweden)

    Chatchai Ratanachai

    2003-05-01

    Full Text Available The classification of trophic conditions of water bodies may often face contradictory cases where a given lake is classified into a trophic category from a trophic variable, whereas it is classified into another trophic category from other trophic variables. To solve this problem, this paper proposes a new methodology based on the concepts of squared Euclidean distance and the boundary values recommended by the OECD (Organization for Economic Cooperation and Development. This methodology requires that a trophic variable data set of a water body under consideration and such boundary values be compared by a measure of similarity computed by using basic statistical techniques to determine the trophic condition of a given water body. The methodology has been tested by applying it to two sample data sets: the Pattani Dam Reservoir and the North Adriatic Sea data sets, which were taken from Kietpawpan (2002 and Zurlini (1996, respectively. The squared Euclidean distance analysis were then applied to the above data sets in order to classifytrophic conditions, based on four trophic variables comprising total nitrogen, total phosphorus, chlorophylla, and Secchi depth. Our results show that the squared Euclidean distance analysis is a useful methodology for preliminarily classifying trophic conditions and solving contradictory classifications, which often arise when applying the present OECD methodology alone.

  9. Folding of non-Euclidean curved shells

    Science.gov (United States)

    Bende, Nakul; Evans, Arthur; Innes-Gold, Sarah; Marin, Luis; Cohen, Itai; Santangelo, Christian; Hayward, Ryan

    2015-03-01

    Origami-based folding of 2D sheets has been of recent interest for a variety of applications ranging from deployable structures to self-folding robots. Though folding of planar sheets follows well-established principles, folding of curved shells involves an added level of complexity due to the inherent influence of curvature on mechanics. In this study, we use principles from differential geometry and thin shell mechanics to establish fundamental rules that govern folding of prototypical creased shells. In particular, we show how the normal curvature of a crease line controls whether the deformation is smooth or discontinuous, and investigate the influence of shell thickness and boundary conditions. We show that snap-folding of shells provides a route to rapid actuation on time-scales dictated by the speed of sound. The simple geometric design principles developed can be applied at any length-scale, offering potential for bio-inspired soft actuators for tunable optics, microfluidics, and robotics. This work was funded by the National Science Foundation through EFRI ODISSEI-1240441 with additional support to S.I.-G. through the UMass MRSEC DMR-0820506 REU program.

  10. Efficient Uplink Modeling for Dynamic System-Level Simulations of Cellular and Mobile Networks

    Directory of Open Access Journals (Sweden)

    Lobinger Andreas

    2010-01-01

    Full Text Available A novel theoretical framework for uplink simulations is proposed. It allows investigations which have to cover a very long (real- time and which at the same time require a certain level of accuracy in terms of radio resource management, quality of service, and mobility. This is of particular importance for simulations of self-organizing networks. For this purpose, conventional system level simulators are not suitable due to slow simulation speeds far beyond real-time. Simpler, snapshot-based tools are lacking the aforementioned accuracy. The runtime improvements are achieved by deriving abstract theoretical models for the MAC layer behavior. The focus in this work is long term evolution, and the most important uplink effects such as fluctuating interference, power control, power limitation, adaptive transmission bandwidth, and control channel limitations are considered. Limitations of the abstract models will be discussed as well. Exemplary results are given at the end to demonstrate the capability of the derived framework.

  11. Thermal conductivity of biological cells at cellular level and correlation with disease state

    Science.gov (United States)

    Park, Byoung Kyoo; Woo, Yunho; Jeong, Dayeong; Park, Jaesung; Choi, Tae-Youl; Simmons, Denise Perry; Ha, Jeonghong; Kim, Dongsik

    2016-06-01

    This paper reports the thermal conductivity k of matched pair cell lines: two pairs of a normal and a cancer cell, one pair of a primary and metastatic cell. The 3ω method with a nanoscale thermal sensor was used to measure k at the single-cell level. To observe the difference in k between normal and cancer cells, the measurements were conducted for Hs 578Bst/Hs 578 T (human breast cells) and TE 353.Sk/TE 354.T (human skin cells). Then k of WM-115/WM-266-4, a primary and metastatic pair of human skin cell, was measured to find the effect of disease progression on k. The measured k data for normal and disease cell samples show statistically meaningful differences. In all cases, k decreased as the disease progressed. This work shows that thermal-analysis schemes, such as the 3ω method, have a potential to detect diseases at the cell level.

  12. Highly Dynamic Cellular-Level Response of Symbiotic Coral to a Sudden Increase in Environmental Nitrogen

    OpenAIRE

    Kopp, C.; M. Pernice; Domart-Coulon, I.; Djediat, C.; J. E. Spangenberg; Alexander, D. T. L.; Hignette, M.; Meziane, T.; Meibom, A.

    2013-01-01

    ABSTRACT Metabolic interactions with endosymbiotic photosynthetic dinoflagellate Symbiodinium spp. are fundamental to reef-building corals (Scleractinia) thriving in nutrient-poor tropical seas. Yet, detailed understanding at the single-cell level of nutrient assimilation, translocation, and utilization within this fundamental symbiosis is lacking. Using pulse-chase 15N labeling and quantitative ion microprobe isotopic imaging (NanoSIMS; nanoscale secondary-ion mass spectrometry), we visualiz...

  13. NAD+-Dependent Deacetylase Hst1p Controls Biosynthesis and Cellular NAD+ Levels in Saccharomyces cerevisiae

    OpenAIRE

    Bedalov, Antonio; Hirao, Maki; Posakony, Jeffrey; Nelson, Melisa; Simon, Julian A.

    2003-01-01

    Nicotine adenine dinucleotide (NAD+) performs key roles in electron transport reactions, as a substrate for poly(ADP-ribose) polymerase and NAD+-dependent protein deacetylases. In the latter two processes, NAD+ is consumed and converted to ADP-ribose and nicotinamide. NAD+ levels can be maintained by regeneration of NAD+ from nicotinamide via a salvage pathway or by de novo synthesis of NAD+ from tryptophan. Both pathways are conserved from yeast to humans. We describe a critical role of the ...

  14. Regulatory Nexus of Synthesis and Degradation Deciphers Cellular Nrf2 Expression Levels

    OpenAIRE

    Suzuki, Takafumi; Shibata, Tatsuhiro; Takaya, Kai; Shiraishi, Kouya; Kohno, Takashi; Kunitoh, Hideo; Tsuta, Koji; Furuta, Koh; Goto, Koichi; Hosoda, Fumie; Sakamoto, Hiromi; Motohashi, Hozumi; Yamamoto, Masayuki

    2013-01-01

    Transcription factor Nrf2 (NF-E2-related factor 2) is essential for oxidative and electrophilic stress responses. While it has been well characterized that Nrf2 activity is tightly regulated at the protein level through proteasomal degradation via Keap1 (Kelch-like ECH-associated protein 1)-mediated ubiquitination, not much attention has been paid to the supply side of Nrf2, especially regulation of Nrf2 gene transcription. Here we report that manipulation of Nrf2 transcription is effective i...

  15. The expression level of the transcription factor Aryl hydrocarbon receptor nuclear translocator (ARNT) determines cellular survival after radiation treatment

    International Nuclear Information System (INIS)

    Tumour hypoxia promotes radioresistance and is associated with poor prognosis. The transcription factor Aryl hydrocarbon receptor nuclear translocator (ARNT), also designated as Hypoxia-inducible factor (HIF)-1β, is part of the HIF pathway which mediates cellular adaptations to oxygen deprivation and facilitates tumour progression. The subunits HIF-1α and ARNT are key players within this pathway. HIF-1α is regulated in an oxygen-dependent manner whereas ARNT is considered to be constitutively expressed. However, there is mounting evidence that certain tumour cells are capable to elevate ARNT in hypoxia which suggests a survival benefit. Therefore the objective of this study was to elucidate effects of an altered ARNT expression level on the cellular response to radiation. Different human cell lines (Hep3B, MCF-7, 786-Owt, 786-Ovhl, RCC4wt and RCC4vhl) originating from various tumour entities (Hepatocellular carcinoma, breast cancer and renal cell carcinoma respectively) were X-irradiated using a conventional linear accelerator. Knockdown of ARNT expression was achieved by transient siRNA transfection. Complementary experiments were performed by forced ARNT overexpression using appropriate plasmids. Presence/absence of ARNT protein was confirmed by Western blot analysis. Clonogenic survival assays were performed in order to determine cellular survival post irradiation. Statistical comparison of two groups was achieved by the unpaired t-test. The results of this study indicate that ARNT depletion renders tumour cells susceptible to radiation whereas overexpression of this transcription factor confers radioresistance. These findings provide evidence to consider ARNT as a drug target and as a predictive marker in clinical applications concerning the response to radiation. The online version of this article (doi:10.1186/s13014-015-0539-9) contains supplementary material, which is available to authorized users

  16. Molecular-Level Tuning of Cellular Autonomy Controls the Collective Behaviors of Cell Populations.

    Science.gov (United States)

    Maire, Théo; Youk, Hyun

    2015-11-25

    A rigorous understanding of how multicellular behaviors arise from the actions of single cells requires quantitative frameworks that bridge the gap between genetic circuits, the arrangement of cells in space, and population-level behaviors. Here, we provide such a framework for a ubiquitous class of multicellular systems-namely, "secrete-and-sense cells" that communicate by secreting and sensing a signaling molecule. By using formal, mathematical arguments and introducing the concept of a phenotype diagram, we show how these cells tune their degrees of autonomous and collective behavior to realize distinct single-cell and population-level phenotypes; these phenomena have biological analogs, such as quorum sensing or paracrine signaling. We also define the "entropy of population," a measurement of the number of arrangements that a population of cells can assume, and demonstrate how a decrease in the entropy of population accompanies the formation of ordered spatial patterns. Our conceptual framework ties together diverse systems, including tissues and microbes, with common principles. PMID:27136241

  17. Monitoring Astronaut Health at the Nanoscale Cellular Level Through the Eye

    Science.gov (United States)

    Ansari, Rafat R.; Singh, Bhim S.; Rovati, Luigi; Docchio, Franco; Sebag, Jerry

    2000-01-01

    A user friendly goggles-like head-mounted device equipped with a suite of instruments for several non-invasive and quantitative medical evaluation of the eye, skin, and brain is desired for monitoring the health of astronauts during space travel and exploration of neighboring and distant planets. Real-time non-invasive evaluation of the different structures within the above organs can provide indices of the health of not just these organs, but the entire body. The techniques such as dynamic light scattering (for the early detection of uveitis, cholesterol levels, cataract, changes in the vitreous and possibly Alzheimer's disease), corneal autofluorescence (to assess extracellular matrix biology e.g., in diabetes), optical activity measurements (of anterior ocular fluid to evaluate blood-glucose levels), laser Doppler velocimetry (to assess retinal, optic nerve, and choroidal blood flow), reflectometry/oximetry (for assessing ocular and central nervous system oxygen metabolism), optical coherence tomography (to determine retinal tissue microstructure) and possibly scanning laser technology (for intraocular tissue imaging and scanning) will he integrated into this compact device. Skin sensors will also be mounted on the portion of the device in contact with the periocular region. This will enable monitoring of body temperature, EEG, and electrolyte status. This device will monitor astronaut health during long-duration space travel by detecting aberrations from pre-established "nonns", enabling prompt diagnosis and possibly the initiation of early preventative/curative therapy. The non-invasive nature of the device technologies permits frequent repetition of tests, enabling real-time complete crew health monitoring. This device may ultimately be useful in tele-medicine to bring modern healthcare to under-served areas on Earth as well as in so-called "advanced" care settings (e.g. diabetes in the USA).

  18. c-Myc and AMPK Control Cellular Energy Levels by Cooperatively Regulating Mitochondrial Structure and Function.

    Directory of Open Access Journals (Sweden)

    Lia R Edmunds

    Full Text Available The c-Myc (Myc oncoprotein and AMP-activated protein kinase (AMPK regulate glycolysis and oxidative phosphorylation (Oxphos although often for different purposes. Because Myc over-expression depletes ATP with the resultant activation of AMPK, we explored the potential co-dependency of and cross-talk between these proteins by comparing the consequences of acute Myc induction in ampk+/+ (WT and ampk-/- (KO murine embryo fibroblasts (MEFs. KO MEFs showed a higher basal rate of glycolysis than WT MEFs and an appropriate increase in response to activation of a Myc-estrogen receptor (MycER fusion protein. However, KO MEFs had a diminished ability to increase Oxphos, mitochondrial mass and reactive oxygen species in response to MycER activation. Other differences between WT and KO MEFs, either in the basal state or following MycER induction, included abnormalities in electron transport chain function, levels of TCA cycle-related oxidoreductases and cytoplasmic and mitochondrial redox states. Transcriptional profiling of pathways pertinent to glycolysis, Oxphos and mitochondrial structure and function also uncovered significant differences between WT and KO MEFs and their response to MycER activation. Finally, an unbiased mass-spectrometry (MS-based survey capable of quantifying ~40% of all mitochondrial proteins, showed about 15% of them to be AMPK- and/or Myc-dependent in their steady state. Significant differences in the activities of the rate-limiting enzymes pyruvate kinase and pyruvate dehydrogenase, which dictate pyruvate and acetyl coenzyme A abundance, were also differentially responsive to Myc and AMPK and could account for some of the differences in basal metabolite levels that were also detected by MS. Thus, Myc and AMPK are highly co-dependent and appear to engage in significant cross-talk across numerous pathways which support metabolic and ATP-generating functions.

  19. In vivo method for the detection of somatic mutations at the cellular level in mice

    Energy Technology Data Exchange (ETDEWEB)

    Searle, A.G.; Stephenson, D.A. (Medical Research Council, Harwell (UK). Radiobiology Unit)

    1982-01-01

    This new method is based on the fact that recessive mutations at the dilute and leaden loci change the shape of pigment-cells in the hair follicles from their normal highly dendritic appearance to a rounded-up almost non-dendritic form. Gene action is autonomous and individual mutant melanocytes can be recognised in whole mounts of dorsal skin made after the post-natal onset of melanogenesis. Foetal heterozygotes for d and In were treated with X-rays (0-200 rad) or procarbazine (0-300 mg/kg body mass by maternal i.p. injection) 17 after detection of a vaginal plug; whole mounts of dorsal skin (fixed 3 after birth) were microscopically scanned for mutant melanocytes in the hair follicles. Mutant cells were mainly in the form of small intrafollicular clones; the few mounts containing large interfollicular clones were excluded from the analysis. For both treatments, dose-response relationships showed good fits to both a quadratic and a power-law curve. However, neither of these differed significantly from a square-law relationship. The estimated mutation rate at the 100 rad X-ray level of 4.7x10/sup -7//rad/locus was in good agreement with the literature. Possible ways in which the present method might be improved and extended are discussed.

  20. Tinnitus: pathology of synaptic plasticity at the cellular and system levels

    Directory of Open Access Journals (Sweden)

    Matthieu J Guitton

    2012-03-01

    Full Text Available Despite being more and more common, and having a high impact on the quality of life of sufferers, tinnitus does not yet have a cure. This has been mostly the result of limited knowledge of the biological mechanisms underlying this adverse pathology. However, the last decade has witnessed tremendous progress in our understanding on the pathophysiology of tinnitus. Animal models have demonstrated that tinnitus is a pathology of neural plasticity, and has two main components: a molecular, peripheral component related to the initiation phase of tinnitus; and a system-level, central component related to the long-term maintenance of tinnitus. Using the most recent experimental data and the molecular/system dichotomy as a framework, we describe here the biological basis of tinnitus. We then discuss these mechanisms from an evolutionary perspective, highlighting similarities with memory. Finally, we consider how these discoveries can translate into therapies, and we suggest operative strategies to design new and effective combined therapeutic solutions using both pharmacological (local and systemic and behavioral tools (e.g., using tele-medicine and virtual reality settings.

  1. Some Properties of Fractals Generated by Linear Cellular Automata

    Institute of Scientific and Technical Information of China (English)

    倪天佳

    2003-01-01

    Fractals and cellular automata are both significant areas of research in nonlinear analysis. This paper studies a class of fractals generated by cellular automata. The patterns produced by cellular automata give a special sequence of sets in Euclidean space. The corresponding limit set is shown to be a fractal and the dimension is independent of the choice of the finite initial seed. As opposed to previous works, the fractals here do not depend on the time parameter.

  2. A nonperturbative foundation of the Euclidean-Minkowskian duality of Wilson-loop correlation functions

    International Nuclear Information System (INIS)

    In this Letter we discuss the analyticity properties of the Wilson-loop correlation functions relevant to the problem of soft high-energy scattering, directly at the level of the functional integral, in a genuinely nonperturbative way. The strategy is to start from the Euclidean theory and to push the dependence on the relevant variables θ (the relative angle between the loops) and T (the half-length of the loops) into the action by means of a field and coordinate transformation, and then to allow them to take complex values. In particular, we determine the analyticity domain of the relevant Euclidean correlation function, and we show that the corresponding Minkowskian quantity is recovered with the usual double analytic continuation in θ and T inside this domain. The formal manipulations of the functional integral are justified making use of a lattice regularisation. The new rescaled action so derived could also be used directly to get new insights (from first principles) in the problem of soft high-energy scattering.

  3. On cohomogeneity one biharmonic hypersurfaces into the Euclidean space

    Science.gov (United States)

    Montaldo, S.; Oniciuc, C.; Ratto, A.

    2016-08-01

    The aim of this paper is to prove that there exists no cohomogeneity one G-invariant proper biharmonic hypersurface into the Euclidean space Rn, where G denotes a transformation group which acts on Rn by isometries, with codimension two principal orbits. This result may be considered in the context of the Chen conjecture, since this family of hypersurfaces includes examples with up to seven distinct principal curvatures. The paper uses the methods of equivariant differential geometry. In particular, the technique of proof provides a unified treatment for all these G-actions.

  4. A Class of Weingarten Surfaces in Euclidean 3-Space

    Directory of Open Access Journals (Sweden)

    Yu Fu

    2013-01-01

    Full Text Available The class of biconservative surfaces in Euclidean 3-space 3 are defined in (Caddeo et al., 2012 by the equation A(grad H=-H grad H for the mean curvature function H and the Weingarten operator A. In this paper, we consider the more general case that surfaces in 3 satisfying A(grad H=kH grad H for some constant k are called generalized bi-conservative surfaces. We show that this class of surfaces are linear Weingarten surfaces. We also give a complete classification of generalized bi-conservative surfaces in 3.

  5. Divisibility tests and recurring decimals in Euclidean domains

    OpenAIRE

    Khare, Apoorva

    2004-01-01

    In this article, we try to explain and unify standard divisibility tests found in various books. We then look at recurring decimals, and list a few of their properties. We show how to compute the number of digits in the recurring part of any fraction. Most of these results are accompanied by a proof (along with the assumptions needed), that works in a Euclidean domain. We then ask some obvious questions related to the results, and mention some similar questions that have been answered. In the...

  6. Phase shift extraction algorithm based on Euclidean matrix norm.

    Science.gov (United States)

    Deng, Jian; Wang, Hankun; Zhang, Desi; Zhong, Liyun; Fan, Jinping; Lu, Xiaoxu

    2013-05-01

    In this Letter, the character of Euclidean matrix norm (EMN) of the intensity difference between phase-shifting interferograms, which changes in sinusoidal form with the phase shifts, is presented. Based on this character, an EMN phase shift extraction algorithm is proposed. Both the simulation calculation and experimental research show that the phase shifts with high precision can be determined with the proposed EMN algorithm easily. Importantly, the proposed EMN algorithm will supply a powerful tool for the rapid calibration of the phase shifts.

  7. Convergent perturbation expansions for Euclidean quantum field theory

    International Nuclear Information System (INIS)

    Mayer perturbation theory is designed to provide computable convergent expansions which permit calculation of Greens functions in Euclidean Quantum Field Theory to arbitrary accuracy, including 'nonperturbative' contributions from large field fluctuations. Here we describe the expansions at the example of 3-dimensional lambdaphi4-theory (in continuous space). They are not essentially more complicated than standard perturbation theory. The n-th order term is expressed in terms of 0(n)-dimensional integrals, and is of order lambda4 if 4k-3<=n<=4k. (orig.)

  8. Engineered mutations in fibrillin-1 leading to Marfan syndrome act at the protein, cellular and organismal levels.

    Science.gov (United States)

    Zeyer, Karina A; Reinhardt, Dieter P

    2015-01-01

    Fibrillins are the major components of microfibrils in the extracellular matrix of elastic and non-elastic tissues. They are multi-domain proteins, containing primarily calcium binding epidermal growth factor-like (cbEGF) domains and 8-cysteine/transforming growth factor-beta binding protein-like (TB) domains. Mutations in the fibrillin-1 gene give rise to Marfan syndrome, a connective tissue disorder with clinical complications in the cardiovascular, skeletal, ocular and other organ systems. Here, we review the consequences of engineered Marfan syndrome mutations in fibrillin-1 at the protein, cellular and organismal levels. Representative point mutations associated with Marfan syndrome in affected individuals have been introduced and analyzed in recombinant fibrillin-1 fragments. Those mutations affect fibrillin-1 on a structural and functional level. Mutations which impair folding of cbEGF domains can affect protein trafficking. Protein folding disrupted by some mutations can lead to defective secretion in mutant fibrillin-1 fragments, whereas fragments with other Marfan mutations are secreted normally. Many Marfan mutations render fibrillin-1 more susceptible to proteolysis. There is also evidence that some mutations affect heparin binding. Few mutations have been further analyzed in mouse models. An extensively studied mouse model of Marfan syndrome expresses mouse fibrillin-1 with a missense mutation (p.C1039G). The mice display similar characteristics to human patients with Marfan syndrome. Overall, the analyses of engineered mutations leading to Marfan syndrome provide important insights into the pathogenic molecular mechanisms exerted by mutated fibrillin-1. PMID:26281765

  9. A medaka model of cancer allowing direct observation of transplanted tumor cells in vivo at a cellular-level resolution.

    Science.gov (United States)

    Hasegawa, Sumitaka; Maruyama, Kouichi; Takenaka, Hikaru; Furukawa, Takako; Saga, Tsuneo

    2009-08-18

    The recent success with small fish as an animal model of cancer with the aid of fluorescence technique has attracted cancer modelers' attention because it would be possible to directly visualize tumor cells in vivo in real time. Here, we report a medaka model capable of allowing the observation of various cell behaviors of transplanted tumor cells, such as cell proliferation and metastasis, which were visualized easily in vivo. We established medaka melanoma (MM) cells stably expressing GFP and transplanted them into nonirradiated and irradiated medaka. The tumor cells were grown at the injection sites in medaka, and the spatiotemporal changes were visualized under a fluorescence stereoscopic microscope at a cellular-level resolution, and even at a single-cell level. Tumor dormancy and metastasis were also observed. Interestingly, in irradiated medaka, accelerated tumor growth and metastasis of the transplanted tumor cells were directly visualized. Our medaka model provides an opportunity to visualize in vivo tumor cells "as seen in a culture dish" and would be useful for in vivo tumor cell biology. PMID:19666513

  10. On the Decidability of Connectedness Constraints in 2D and 3D Euclidean Spaces

    CERN Document Server

    Kontchakov, Roman; Pratt-Hartmann, Ian; Zakharyaschev, Michael

    2011-01-01

    We investigate (quantifier-free) spatial constraint languages with equality, contact and connectedness predicates as well as Boolean operations on regions, interpreted over low-dimensional Euclidean spaces. We show that the complexity of reasoning varies dramatically depending on the dimension of the space and on the type of regions considered. For example, the logic with the interior-connectedness predicate (and without contact) is undecidable over polygons or regular closed sets in the Euclidean plane, NP-complete over regular closed sets in three-dimensional Euclidean space, and ExpTime-complete over polyhedra in three-dimensional Euclidean space.

  11. The Einstein Constraint Equations on Asymptotically Euclidean Manifolds

    CERN Document Server

    Dilts, James

    2015-01-01

    In this dissertation, we prove a number of results regarding the conformal method of finding solutions to the Einstein constraint equations. These results include necessary and sufficient conditions for the Lichnerowicz equation to have solutions, global supersolutions which guarantee solutions to the conformal constraint equations for near-constant-mean-curvature (near-CMC) data as well as for far-from-CMC data, a proof of the limit equation criterion in the near-CMC case, as well as a model problem on the relationship between the asymptotic constants of solutions and the ADM mass. We also prove a characterization of the Yamabe classes on asymptotically Euclidean manifolds and resolve the (conformally) prescribed scalar curvature problem on asymptotically Euclidean manifolds for the case of nonpositive scalar curvatures. Many, though not all, of the results in this dissertation have been previously published in [Dilts13b], [DIMM14], [DL14], [DM15], and [DGI15]. This article is the author's Ph.D. dissertation...

  12. Notes on Euclidean Wilson loops and Riemann Theta functions

    CERN Document Server

    Ishizeki, Riei; Ziama, Sannah

    2011-01-01

    The AdS/CFT correspondence relates Wilson loops in N=4 SYM theory to minimal area surfaces in AdS5 space. In this paper we consider the case of Euclidean flat Wilson loops which are related to minimal area surfaces in Euclidean AdS3 space. Using known mathematical results for such minimal area surfaces we describe an infinite parameter family of analytic solutions for closed Wilson loops. The solutions are given in terms of Riemann theta functions and the validity of the equations of motion is proven based on the trisecant identity. A relatively simple formula is derived for the renormalized area and an example is discussed in detail with plots of the corresponding surface. For each Wilson loops we explicitly construct a one parameter family of deformations that preserve the area. The parameter is the so called spectral parameter. Furthermore, for genus three we find a map between these Wilson loops and closed curves inside the Riemann surface.

  13. Euclidean resonance and a new type of nuclear reactions

    CERN Document Server

    Ivlev, B I

    2003-01-01

    The extremely small probability of quantum tunneling through an almost classical potential barrier may become not small under the action of the specially adapted nonstationary field. The tunneling rate has a sharp peak as a function of the particle energy when it is close to the certain resonant value defined by the nonstationary field (Euclidean resonance). Alpha decay of nuclei has a small probability since the alpha particle should tunnel through a very nontransparent Coulomb barrier. The incident proton, due to the Coulomb interaction with the tunneling alpha particle, plays the role of a nonstationary field which may result in Euclidean resonance in tunneling of the alpha particle. At the resonant proton energy, which is of the order of 0.2 Mev, the alpha particle escapes the nucleus and goes to infinity with no influence of the nuclear Coulomb barrier. The process is inelastic since the alpha particle releases energy and the proton gains it. This stimulation of alpha decay by a proton constitutes a new ...

  14. Thermodynamics, Euclidean Gravity and Kaluza-Klein Reduction

    CERN Document Server

    Fursaev, D V

    2001-01-01

    The aim of this paper is to find out a correspondence between one-loop effective action $W_E$ defined by means of path integral in Euclidean gravity and the free energy $F$ obtained by summation over the modes. The analysis is given for quantum fields on stationary space-times of a general form. For such problems a convenient procedure of a "Wick rotation" from Euclidean to Lorentzian theory becomes quite non-trivial implying transition from one real section of a complexified space-time manifold to another. We formulate conditions under which $F$ and $W_E$ can be connected and establish an explicit relation of these functionals. Our results are based on the Kaluza-Klein method which enables one to reduce the problem on a stationary space-time to equivalent problem on a static space-time in the presence of a gauge connection. As a by-product, we discover relation between the asymptotic heat-kernel coefficients of elliptic operators on a $D$ dimensional stationary space-times and the heat-kernel coefficients of...

  15. Platelet-Rich Plasma Increases the Levels of Catabolic Molecules and Cellular Dedifferentiation in the Meniscus of a Rabbit Model

    Directory of Open Access Journals (Sweden)

    Hye-Rim Lee

    2016-01-01

    Full Text Available Despite the susceptibility to frequent intrinsic and extrinsic injuries, especially in the inner zone, the meniscus does not heal spontaneously owing to its poor vascularity. In this study, the effect of platelet-rich plasma (PRP, containing various growth factors, on meniscal mechanisms was examined under normal and post-traumatic inflammatory conditions. Isolated primary meniscal cells of New Zealand white (NZW rabbits were incubated for 3, 10, 14 and 21 days with PRP(−, 10% PRP (PRP(+, IL(+ or IL(+PRP(+. The meniscal cells were collected and examined using reverse-transcription polymerase chain reaction (RT-PCR. Culture media were examined by immunoblot analyses for matrix metalloproteinases (MMP catabolic molecules. PRP containing growth factors improved the cellular viability of meniscal cells in a concentration-dependent manner at Days 1, 4 and 7. However, based on RT-PCR, meniscal cells demonstrated dedifferentiation, along with an increase in type I collagen in the PRP(+ and in IL(+PRP(+. In PRP(+, the aggrecan expression levels were lower than in the PRP(− until Day 21. The protein levels of MMP-1 and MMP-3 were higher in each PRP group, i.e., PRP(+ and IL(+PRP(+, at each culture time. A reproducible 2-mm circular defect on the meniscus of NZW rabbit was used to implant fibrin glue (control or PRP in vivo. After eight weeks, the lesions in the control and PRP groups were occupied with fibrous tissue, but not with meniscal cells. This study shows that PRP treatment of the meniscus results in an increase of catabolic molecules, especially those related to IL-1α-induced inflammation, and that PRP treatment for an in vivo meniscus injury accelerates fibrosis, instead of meniscal cartilage.

  16. Increased Cellular NAD+ Level through NQO1 Enzymatic Action Has Protective Effects on Bleomycin-Induced Lung Fibrosis in Mice

    Science.gov (United States)

    Oh, Gi-Su; Lee, Su-Bin; Karna, Anjani; Kim, Hyung-Jin; Shen, AiHua; Pandit, Arpana; Lee, SeungHoon

    2016-01-01

    Background Idiopathic pulmonary fibrosis is a common interstitial lung disease; it is a chronic, progressive, and fatal lung disease of unknown etiology. Over the last two decades, knowledge about the underlying mechanisms of pulmonary fibrosis has improved markedly and facilitated the identification of potential targets for novel therapies. However, despite the large number of antifibrotic drugs being described in experimental pre-clinical studies, the translation of these findings into clinical practices has not been accomplished yet. NADH:quinone oxidoreductase 1 (NQO1) is a homodimeric enzyme that catalyzes the oxidation of NADH to NAD+ by various quinones and thereby elevates the intracellular NAD+ levels. In this study, we examined the effect of increase in cellular NAD+ levels on bleomycin-induced lung fibrosis in mice. Methods C57BL/6 mice were treated with intratracheal instillation of bleomycin. The mice were orally administered with β-lapachone from 3 days before exposure to bleomycin to 1-3 weeks after exposure to bleomycin. Bronchoalveolar lavage fluid (BALF) was collected for analyzing the infiltration of immune cells. In vitro, A549 cells were treated with transforming growth factor β1 (TGF-β1) and β-lapachone to analyze the extracellular matrix (ECM) and epithelial-mesenchymal transition (EMT). Results β-Lapachone strongly attenuated bleomycin-induced lung inflammation and fibrosis, characterized by histological staining, infiltrated immune cells in BALF, inflammatory cytokines, fibrotic score, and TGF-β1, α-smooth muscle actin accumulation. In addition, β-lapachone showed a protective role in TGF-β1–induced ECM expression and EMT in A549 cells. Conclusion Our results suggest that β-lapachone can protect against bleomycin-induced lung inflammation and fibrosis in mice and TGF-β1–induced EMT in vitro, by elevating the NAD+/NADH ratio through NQO1 activation.

  17. Elevated serum levels of T3 without metabolic effect in nutritionally deficient rats, attributable to reduced cellular uptake of T3

    International Nuclear Information System (INIS)

    Rats receiving a nutritionally deficient diet displayed markedly elevated serum free T3 levels but showed no increase in oxygen consumption. This was associated with greatly reduced ratios of hepatic cellular and nuclear /sub 125/I-T3 to serum /sub 125/I-T3. Kinetic data supported the conclusion that cellular uptake of T3 was decreased in the nutritionally deficient rats. The lack of metabolic effect, despite the elevated serum T3 levels, is attributable to reduced availability of serum T3 to tissue nuclear receptor sites

  18. BPS Wilson loops in Minkowski spacetime and Euclidean space

    Energy Technology Data Exchange (ETDEWEB)

    Ouyang, Hao; Wu, Jun-Bao; Zhang, Jia-ju [Chinese Academy of Sciences, Theoretical Physics Division, Institute of High Energy Physics, Beijing (China); Chinese Academy of Sciences, Theoretical Physics Center for Science Facilities, Beijing (China)

    2015-12-15

    We give evidence that spacelike BPS Wilson loops do not exist in Minkowski spacetime. We show that spacelikeWilson loops in Minkowski spacetime cannot preserve any supersymmetries, in d = 4 N = 4 super Yang-Mills theory, d = 3 N = 2 super Chern-Simons-matter theory, and d = 3 N = 6 Aharony-Bergman-Jafferis- Maldacena theory. We not only show this using infinite straight lines and circles as examples, but also we give proofs for general curves. We attribute this to the conflicts of the reality conditions of the spinors. However, spacelike Wilson loops do exist in Euclidean space. There are both BPS Wilson loops along infinite straight lines and circular BPS Wilson loops. This is because the reality conditions of the spinors are lost after Wick rotation. The result is reasonable in view of the AdS/CFT correspondence. (orig.)

  19. BPS Wilson loops in Minkowski spacetime and Euclidean space

    Science.gov (United States)

    Ouyang, Hao; Wu, Jun-Bao; Zhang, Jia-ju

    2015-12-01

    We give evidence that spacelike BPS Wilson loops do not exist in Minkowski spacetime. We show that spacelike Wilson loops in Minkowski spacetime cannot preserve any supersymmetries, in d = 4 N = 4 super Yang-Mills theory, d = 3 N = 2 super Chern-Simons-matter theory, and d = 3 N = 6 Aharony-Bergman-Jafferis-Maldacena theory. We not only show this using infinite straight lines and circles as examples, but also we give proofs for general curves. We attribute this to the conflicts of the reality conditions of the spinors. However, spacelike Wilson loops do exist in Euclidean space. There are both BPS Wilson loops along infinite straight lines and circular BPS Wilson loops. This is because the reality conditions of the spinors are lost after Wick rotation. The result is reasonable in view of the AdS/CFT correspondence.

  20. On the high-density expansion for Euclidean random matrices

    Science.gov (United States)

    Grigera, T. S.; Martin-Mayor, V.; Parisi, G.; Urbani, P.; Verrocchio, P.

    2011-02-01

    Diagrammatic techniques to compute perturbatively the spectral properties of Euclidean random matrices (ERM) in the high-density regime are introduced and discussed in detail. Such techniques are developed in two alternative and very different formulations of the mathematical problem and are shown to give identical results up to second order in the perturbative expansion. One method, based on writing the so-called resolvent function as a Taylor series, allows us to group the diagrams into a small number of topological classes, providing a simple way to determine the infrared (small momenta) behaviour of the theory up to third order, which is of interest for the comparison with experiments. The other method, which reformulates the problem as a field theory, can instead be used to study the infrared behaviour at any perturbative order.

  1. On continuous expansions of configurations of points in Euclidean space

    CERN Document Server

    Cheng, Holun; Zheng, Yidan

    2011-01-01

    For any two configurations of ordered points $p=(p_{1},...,\\p_{N})$ and $q=(q_{1},...,q_{N})$ in Euclidean space $E^d$ such that $q$ is an expansion of $p$, there exists a continuous expansion from $p$ to $q$ in dimension 2d; Bezdek and Connelly used this to prove the Kneser-Poulsen conjecture for the planar case. In this paper, we show that this construction is optimal in the sense that for any $d \\ge 2$ there exists configurations of $(d+1)^2$ points $p$ and $q$ in $E^d$ such that $q$ is an expansion of $p$ but there is no continuous expansion from $p$ to $q$ in dimension less than 2d. The techniques used in our proof are completely elementary.

  2. Susceptible-Infected-Recovered model on Euclidean network

    CERN Document Server

    Khaleque, Abdul

    2012-01-01

    We consider the Susceptible-Infected-Recovered (SIR) epidemic model on a Euclidean network in one dimension in which nodes at a distance $l$ are connected with probability $P(l) \\propto l^{-\\delta}$ in addition to nearest neighbors. The topology of the network changes as $\\delta$ is varied and its effect on the SIR model is studied. $R(t)$, the recovered fraction of population up to time $t$, and $\\tau$, the total duration of the epidemic are calculated for different values of the infection probability $q$ and $\\delta$. A threshold behavior is observed for all $\\delta$ up to $\\delta \\approx 2.0$; above the threshold value $q = q_c$, the saturation value $R_{sat}$ attains a finite value. Both $R_{sat}$ and $\\tau $ show scaling behavior in a finite system of size $N$; $R_{sat} \\sim N^{-\\beta/{\\tilde{\

  3. Sensor Network Localization by Eigenvector Synchronization Over the Euclidean Group.

    Science.gov (United States)

    Cucuringu, Mihai; Lipman, Yaron; Singer, Amit

    2012-07-01

    We present a new approach to localization of sensors from noisy measurements of a subset of their Euclidean distances. Our algorithm starts by finding, embedding, and aligning uniquely realizable subsets of neighboring sensors called patches. In the noise-free case, each patch agrees with its global positioning up to an unknown rigid motion of translation, rotation, and possibly reflection. The reflections and rotations are estimated using the recently developed eigenvector synchronization algorithm, while the translations are estimated by solving an overdetermined linear system. The algorithm is scalable as the number of nodes increases and can be implemented in a distributed fashion. Extensive numerical experiments show that it compares favorably to other existing algorithms in terms of robustness to noise, sparse connectivity, and running time. While our approach is applicable to higher dimensions, in the current article, we focus on the two-dimensional case.

  4. Entangling gates in even Euclidean lattices such as Leech lattice

    CERN Document Server

    Planat, Michel

    2010-01-01

    We point out a organic relationship between real entangling n-qubit gates of quantum computation and the group of automorphisms of even Euclidean lattices of the corresponding dimension 2n. The type of entanglement that is found in the gates/generators of Aut() depends on the lattice. In particular, we investigate Zn lattices, Barnes-Wall lattices D4, E8, 16 (associated to n = 2, 3 and 4 qubits), and the Leech lattices h24 and 24 (associated to a 3-qubit/qutrit system). Balanced tripartite entanglement is found to be a basic feature of Aut(), a nding that bears out our recent work related to the Weyl group of E8 [1, 2].

  5. BPS Wilson loops in Minkowski spacetime and Euclidean space

    Energy Technology Data Exchange (ETDEWEB)

    Ouyang, Hao, E-mail: ouyangh@ihep.ac.cn; Wu, Jun-Bao, E-mail: wujb@ihep.ac.cn; Zhang, Jia-ju, E-mail: jjzhang@ihep.ac.cn [Theoretical Physics Division, Institute of High Energy Physics, Chinese Academy of Sciences, 19B Yuquan Rd, 100049, Beijing (China); Theoretical Physics Center for Science Facilities, Chinese Academy of Sciences, 19B Yuquan Rd, 100049, Beijing (China)

    2015-12-21

    We give evidence that spacelike BPS Wilson loops do not exist in Minkowski spacetime. We show that spacelike Wilson loops in Minkowski spacetime cannot preserve any supersymmetries, in d=4N=4 super Yang–Mills theory, d=3N=2 super Chern–Simons-matter theory, and d=3N=6 Aharony–Bergman–Jafferis–Maldacena theory. We not only show this using infinite straight lines and circles as examples, but also we give proofs for general curves. We attribute this to the conflicts of the reality conditions of the spinors. However, spacelike Wilson loops do exist in Euclidean space. There are both BPS Wilson loops along infinite straight lines and circular BPS Wilson loops. This is because the reality conditions of the spinors are lost after Wick rotation. The result is reasonable in view of the AdS/CFT correspondence.

  6. Application of Euclidean distance measurement and principal component analysis for gene identification.

    Science.gov (United States)

    Ghosh, Antara; Barman, Soma

    2016-06-01

    Gene systems are extremely complex, heterogeneous, and noisy in nature. Many statistical tools which are used to extract relevant feature from genes provide fuzzy and ambiguous information. High-dimensional gene expression database available in public domain usually contains thousands of genes. Efficient prediction method is demanding nowadays for accurate identification of such database. Euclidean distance measurement and principal component analysis methods are applied on such databases to identify the genes. In both methods, prediction algorithm is based on homology search approach. Digital Signal Processing technique along with statistical method is used for analysis of genes in both cases. A two-level decision logic is used for gene classification as healthy or cancerous. This binary logic minimizes the prediction error and improves prediction accuracy. Superiority of the method is judged by receiver operating characteristic curve.

  7. 10D to 4D Euclidean Supergravity over a Calabi-Yau three-fold

    CERN Document Server

    Sabra, Wafic

    2015-01-01

    We dimensionally reduce the bosonic sector of 10D Euclidean type IIA supergravity over a Calabi-Yau three-fold. The resulting theory describes the bosonic sector of 4D, N = 2 Euclidean supergravity coupled to vector- and hyper-multiplets.

  8. Three Dimensional Fast Exact Euclidean Distance (3D-FEED) Maps

    NARCIS (Netherlands)

    Schouten, Theo E.; Kuppens, Harco C.; Broek, van den Egon L.

    2006-01-01

    In image and video analysis, distance maps are frequently used. They provide the (Euclidean) distance (ED) of background pixels to the nearest object pixel. Recently, the Fast Exact Euclidean Distance (FEED) transformation was launched. In this paper, we present the three dimensional (3D) version of

  9. Balancing Degree, Diameter and Weight in Euclidean Spanners

    CERN Document Server

    Solomon, Shay

    2011-01-01

    In this paper we devise a novel \\emph{unified} construction of Euclidean spanners that trades between the maximum degree, diameter and weight gracefully. For a positive integer k, our construction provides a (1+eps)-spanner with maximum degree O(k), diameter O(log_k n + alpha(k)), weight O(k \\cdot log_k n \\cdot log n) \\cdot w(MST(S)), and O(n) edges. Note that for k= n^{1/alpha(n)} this gives rise to diameter O(alpha(n)), weight O(n^{1/alpha(n)} \\cdot log n \\cdot alpha(n)) \\cdot w(MST(S)) and maximum degree O(n^{1/alpha(n)}), which improves upon a classical result of Arya et al. \\cite{ADMSS95}; in the corresponding result from \\cite{ADMSS95} the spanner has the same number of edges and diameter, but its weight and degree may be arbitrarily large. Also, for k = O(1) this gives rise to maximum degree O(1), diameter O(log n) and weight O(log^2 n) \\cdot w(MST(S)), which reproves another classical result of Arya et al. \\cite{ADMSS95}. Our bound of O(log_k n + alpha(k)) on the diameter is optimal under the constrai...

  10. Algebraic field descriptions in three-dimensional Euclidean space

    Science.gov (United States)

    Salingaros, Nikos; Ilamed, Yehiel

    1984-08-01

    In this paper, we use the differential forms of three-dimensional Euclidean space to realize a Clifford algebra which is isomorphic to the algebra of the Pauli matrices or the complex quaternions. This is an associative vector-antisymmetric tensor algebra with division: We provide the algebraic inverse of an eight-component spinor field which is the sum of a scalar + vector + pseudovector + pseudoscalar. A surface of singularities is defined naturally by the inverse of an eight-component spinor and corresponds to a generalized Minkowski “double” light cone in the parameter space. A general description of finite spatial rotations, which utilizes the Baker-Campbell-Hausdorff formula, generalizes the usual infinitesimal treatments of the rotation group. We derive an explicit expression for the angle corresponding to two successive finite rotations in any direction. We also discuss Lorentz transformations and duality rotations of the electromagnetic field and exhibit a relationship between the algebraic inverse and a duality rotated field. Using a combined transformation, one can always transform an arbitrary electromagnetic field ( E≠0) into a pure electric field, but never into a pure magnetic field.

  11. Generating triangulated macromolecular surfaces by Euclidean Distance Transform.

    Directory of Open Access Journals (Sweden)

    Dong Xu

    Full Text Available Macromolecular surfaces are fundamental representations of their three-dimensional geometric shape. Accurate calculation of protein surfaces is of critical importance in the protein structural and functional studies including ligand-protein docking and virtual screening. In contrast to analytical or parametric representation of macromolecular surfaces, triangulated mesh surfaces have been proved to be easy to describe, visualize and manipulate by computer programs. Here, we develop a new algorithm of EDTSurf for generating three major macromolecular surfaces of van der Waals surface, solvent-accessible surface and molecular surface, using the technique of fast Euclidean Distance Transform (EDT. The triangulated surfaces are constructed directly from volumetric solids by a Vertex-Connected Marching Cube algorithm that forms triangles from grid points. Compared to the analytical result, the relative error of the surface calculations by EDTSurf is <2-4% depending on the grid resolution, which is 1.5-4 times lower than the methods in the literature; and yet, the algorithm is faster and costs less computer memory than the comparative methods. The improvements in both accuracy and speed of the macromolecular surface determination should make EDTSurf a useful tool for the detailed study of protein docking and structure predictions. Both source code and the executable program of EDTSurf are freely available at http://zhang.bioinformatics.ku.edu/EDTSurf.

  12. Phg1/TM9 proteins control intracellular killing of bacteria by determining cellular levels of the Kil1 sulfotransferase in Dictyostelium.

    Directory of Open Access Journals (Sweden)

    Marion Le Coadic

    Full Text Available Dictyostelium discoideum has largely been used to study phagocytosis and intracellular killing of bacteria. Previous studies have shown that Phg1A, Kil1 and Kil2 proteins are necessary for efficient intracellular killing of Klebsiella bacteria. Here we show that in phg1a KO cells, cellular levels of lysosomal glycosidases and lysozyme are decreased, and lysosomal pH is increased. Surprisingly, overexpression of Kil1 restores efficient killing in phg1a KO cells without correcting these lysosomal anomalies. Conversely, kil1 KO cells are defective for killing, but their enzymatic content and lysosomal pH are indistinguishable from WT cells. The killing defect of phg1a KO cells can be accounted for by the observation that in these cells the stability and the cellular amount of Kil1 are markedly reduced. Since Kil1 is the only sulfotransferase characterized in Dictyostelium, an (unidentified sulfated factor, defective in both phg1a and kil1 KO cells, may play a key role in intracellular killing of Klebsiella bacteria. In addition, Phg1B plays a redundant role with Phg1A in controlling cellular amounts of Kil1 and intracellular killing. Finally, cellular levels of Kil1 are unaffected in kil2 KO cells, and Kil1 overexpression does not correct the killing defect of kil2 KO cells, suggesting that Kil2 plays a distinct role in intracellular killing.

  13. Development of liquid chromatography mass spectrometric methods for quantification of metabolites from cellular level to clinical biomarkers

    OpenAIRE

    Tohmola, Niina

    2015-01-01

    Metabolites are low molecular weight compounds participating in different functions of cellular systems. Metabolites can be used as diagnostic biomarkers for numerous diseases. Liquid chromatography tandem mass spectrometry (LC-MS/MS) is a powerful tool in quantification of metabolites from various sample matrices. Good sensitivity and specificity are the main benefits of the technique. Mass spectrometry is commonly used in industry, drug research and clinical diagnostics. Extensive validatio...

  14. Asymptotic analysis of fundamental solutions of Dirac operators on even dimensional Euclidean spaces

    International Nuclear Information System (INIS)

    We analyze the short distance asymptotic behavior of some quantities formed out of fundamental solutions of Dirac operators on even dimensional Euclidean spaces with finite dimensional matrix-valued potentials. (orig.)

  15. Large parallel volumes of finite and compact sets in d-dimensional Euclidean space

    DEFF Research Database (Denmark)

    Kampf, Jürgen; Kiderlen, Markus

    The r-parallel volume V (Cr) of a compact subset C in d-dimensional Euclidean space is the volume of the set Cr of all points of Euclidean distance at most r > 0 from C. According to Steiner’s formula, V (Cr) is a polynomial in r when C is convex. For finite sets C satisfying a certain geometric ......, it is shown that the difference of large r-parallel volumes of C and of its convex hull behaves like crd−3, where c is an intrinsic power volume of C.......The r-parallel volume V (Cr) of a compact subset C in d-dimensional Euclidean space is the volume of the set Cr of all points of Euclidean distance at most r > 0 from C. According to Steiner’s formula, V (Cr) is a polynomial in r when C is convex. For finite sets C satisfying a certain geometric...

  16. Singularities of lightlike hypersurface in semi-Euclidean 4-space with index 2

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Anti de Sitter space is a maximally symmetric, vacuum solution of Einstein’s field equation with an attractive cosmological constant, and is the hyperquadric of semi-Euclidean space with index 2. So it is meaningful to study the submanifold in semi-Euclidean 4-space with index 2. However, the research on the submanifold in semi-Euclidean 4-space with index 2 has not been found from theory of singularity until now. In this paper, as a generalization of the study on lightlike hypersurface in Minkowski space and a preparation for the further study on anti de Sitter space, the singularities of lightlike hypersurface and Lorentzian surface in semi- Euclidean 4-space with index 2 will be studied. To do this, we reveal the relationships between the singularity of distance-squared function and that of lightlike hypersurface. In addition some geometric properties of lightlike hypersurface and Lorentzian surface are studied from geometrical point of view.

  17. Euclidean Geometry Codes, minimum weight words and decodable error-patterns using bit-flipping

    DEFF Research Database (Denmark)

    Høholdt, Tom; Justesen, Jørn; Jonsson, Bergtor

    2005-01-01

    We determine the number of minimum wigth words in a class of Euclidean Geometry codes and link the performance of the bit-flipping decoding algorithm to the geometry of the error patterns.......We determine the number of minimum wigth words in a class of Euclidean Geometry codes and link the performance of the bit-flipping decoding algorithm to the geometry of the error patterns....

  18. No evident dose-response relationship between cellular ROS level and its cytotoxicity--a paradoxical issue in ROS-based cancer therapy.

    Science.gov (United States)

    Zhu, Chunpeng; Hu, Wei; Wu, Hao; Hu, Xun

    2014-01-01

    Targeting cancer via ROS-based mechanism has been proposed as a radical therapeutic approach. Cancer cells exhibit higher endogenous oxidative stress than normal cells and pharmacological ROS insults via either enhancing ROS production or inhibiting ROS-scavenging activity can selectively kill cancer cells. In this study, we randomly chose 4 cancer cell lines and primary colon or rectal cancer cells from 4 patients to test the hypothesis and obtained following paradoxical results: while piperlongumin (PL) and β-phenylethyl isothiocyanate (PEITC), 2 well-defined ROS-based anticancer agents, induced an increase of cellular ROS and killed effectively the tested cells, lactic acidosis (LA), a common tumor environmental factor that plays multifaceted roles in promoting cancer progression, induced a much higher ROS level in the tested cancer cells than PL and PEITC, but spared them; L-buthionine sulfoximine (L-BSO, 20 μM) depleted cellular GSH more effectively and increased higher ROS level than PL or PEITC but permitted progressive growth of the tested cancer cells. No evident dose-response relationship between cellular ROS level and cytotoxicity was observed. If ROS is the effecter, it should obey the fundamental therapeutic principle - the dose-response relationship. This is a major concern. PMID:24848642

  19. Modulation of cellular Hsp72 levels in undifferentiated and neuron-like SH-SY5Y cells determines resistance to staurosporine-induced apoptosis.

    Directory of Open Access Journals (Sweden)

    Lesley Cheng

    Full Text Available Increased expression of Hsp72 accompanies differentiation of human neuroblastoma SH-SY5Y cells to neuron-like cells. By modulating cellular levels of Hsp72, we demonstrate here its anti-apoptotic activity both in undifferentiated and neuron-like cells. Thermal preconditioning (43°C for 30 min induced Hsp72, leading to cellular protection against apoptosis induced by a subsequent treatment with staurosporine. Preconditioned staurosporine-treated cells displayed decreased Bax recruitment to mitochondria and subsequent activation, as well as reduced cytochrome c redistribution from mitochondria. The data are consistent with Hsp72 blocking apoptosis upstream of Bax recruitment to mitochondria. Neuron-like cells (with elevated Hsp72 were more resistant to staurosporine by all measured indices of apoptotic signaling. Use of stable transfectants ectopically expressing moderately elevated levels of Hsp72 revealed that such cells in the undifferentiated state showed enhanced resistance to staurosporine-induced apoptosis, which was even more robust after differentiation to neuron-like cells. Overall, the protective effects of differentiation, thermal preconditioning and ectopic Hsp72 expression were additive. The strong inverse correlation between cellular Hsp72 levels and susceptibility to apoptosis support the notion that Hsp72 acts as a significant neuroprotective factor, enabling post-mitotic neurons to withstand potentially lethal stress that induces apoptosis.

  20. Multi-resolutional shape features via non-Euclidean wavelets: Applications to statistical analysis of cortical thickness

    Science.gov (United States)

    Kim, Won Hwa; Singh, Vikas; Chung, Moo K.; Hinrichs, Chris; Pachauri, Deepti; Okonkwo, Ozioma C.; Johnson, Sterling C.

    2014-01-01

    Statistical analysis on arbitrary surface meshes such as the cortical surface is an important approach to understanding brain diseases such as Alzheimer’s disease (AD). Surface analysis may be able to identify specific cortical patterns that relate to certain disease characteristics or exhibit differences between groups. Our goal in this paper is to make group analysis of signals on surfaces more sensitive. To do this, we derive multi-scale shape descriptors that characterize the signal around each mesh vertex, i.e., its local context, at varying levels of resolution. In order to define such a shape descriptor, we make use of recent results from harmonic analysis that extend traditional continuous wavelet theory from the Euclidean to a non-Euclidean setting (i.e., a graph, mesh or network). Using this descriptor, we conduct experiments on two different datasets, the Alzheimer’s Disease NeuroImaging Initiative (ADNI) data and images acquired at the Wisconsin Alzheimer’s Disease Research Center (W-ADRC), focusing on individuals labeled as having Alzheimer’s disease (AD), mild cognitive impairment (MCI) and healthy controls. In particular, we contrast traditional univariate methods with our multi-resolution approach which show increased sensitivity and improved statistical power to detect a group-level effects. We also provide an open source implementation. PMID:24614060

  1. Combined effects of gamma irradiation and cadmium on cellular and population-level endpoints of the micro-alga Pseudokirchneriella subcapitata

    Energy Technology Data Exchange (ETDEWEB)

    Bradshaw, C. [Stockholm University (Sweden); Abdul Meseh, D.; Alasawi, H.; Qiang, M.; Nascimento, F. [Dept of Ecology, Environment and Plant Sciences (Sweden)

    2014-07-01

    A major challenge in evaluating the risks of radiation to organisms is that radioactive substances often co-occur with other contaminants in the environment. The combined effects of multiple contaminants is poorly understood, particularly where radiation is involved, but mixture toxicity can give rise to synergistic, antagonistic or additive effects. The challenge of understanding mixture toxicity in a radiation context is the focus of one of the work packages of the STAR EU Network of Excellence in Radioecology, of which this study is a part. This paper presents results from an experiment where the green micro-alga Pseudokirchneriella subcapitata was exposed to both acute external gamma irradiation and the toxic metal cadmium (Cd) (over 72 hours); the experiment had a fully factorial design with 4 gamma doses and 4 Cd concentrations. The endpoints measured were chosen to reflect subcellular, cellular and population-level effects: antioxidant enzyme expression; membrane damage; protein, vitamin and pigment content of the cells; individual cell biomass and growth; population growth (biomass per ml and cells per ml). Preliminary results suggest effects of both Cd and gamma on some of the cellular and subcellular endpoints such as thiamine (vitamin B1) and chlorophyll concentrations in the cells, and individual cell biomass. In some cases interactive effects of the combined Cd and gamma treatments were seen, and these appeared to be dose level dependent. This lack of a consistent pattern of interactive mixture toxicity effects across the endpoints measured means that such effects would be very hard to predict in a risk assessment context. The lack of measurable effects at the population level was probably due to the short experimental duration (72 hours). Other experiments in our research group on the same micro-alga species that have looked at longer term effects (weeks) have shown that effects may not manifest themselves until at least a week after an acute gamma

  2. Confidence analysis of standard deviational ellipse and its extension into higher dimensional euclidean space.

    Science.gov (United States)

    Wang, Bin; Shi, Wenzhong; Miao, Zelang

    2015-01-01

    Standard deviational ellipse (SDE) has long served as a versatile GIS tool for delineating the geographic distribution of concerned features. This paper firstly summarizes two existing models of calculating SDE, and then proposes a novel approach to constructing the same SDE based on spectral decomposition of the sample covariance, by which the SDE concept is naturally generalized into higher dimensional Euclidean space, named standard deviational hyper-ellipsoid (SDHE). Then, rigorous recursion formulas are derived for calculating the confidence levels of scaled SDHE with arbitrary magnification ratios in any dimensional space. Besides, an inexact-newton method based iterative algorithm is also proposed for solving the corresponding magnification ratio of a scaled SDHE when the confidence probability and space dimensionality are pre-specified. These results provide an efficient manner to supersede the traditional table lookup of tabulated chi-square distribution. Finally, synthetic data is employed to generate the 1-3 multiple SDEs and SDHEs. And exploratory analysis by means of SDEs and SDHEs are also conducted for measuring the spread concentrations of Hong Kong's H1N1 in 2009.

  3. Confidence analysis of standard deviational ellipse and its extension into higher dimensional euclidean space.

    Directory of Open Access Journals (Sweden)

    Bin Wang

    Full Text Available Standard deviational ellipse (SDE has long served as a versatile GIS tool for delineating the geographic distribution of concerned features. This paper firstly summarizes two existing models of calculating SDE, and then proposes a novel approach to constructing the same SDE based on spectral decomposition of the sample covariance, by which the SDE concept is naturally generalized into higher dimensional Euclidean space, named standard deviational hyper-ellipsoid (SDHE. Then, rigorous recursion formulas are derived for calculating the confidence levels of scaled SDHE with arbitrary magnification ratios in any dimensional space. Besides, an inexact-newton method based iterative algorithm is also proposed for solving the corresponding magnification ratio of a scaled SDHE when the confidence probability and space dimensionality are pre-specified. These results provide an efficient manner to supersede the traditional table lookup of tabulated chi-square distribution. Finally, synthetic data is employed to generate the 1-3 multiple SDEs and SDHEs. And exploratory analysis by means of SDEs and SDHEs are also conducted for measuring the spread concentrations of Hong Kong's H1N1 in 2009.

  4. Modelling molecular mechanisms: a framework of scientific reasoning to construct molecular-level explanations for cellular behaviour

    NARCIS (Netherlands)

    van Mil, M.H.W.; Boerwinkel, D.J.; Waarlo, A.J.

    2013-01-01

    Although molecular-level details are part of the upper-secondary biology curriculum in most countries, many studies report that students fail to connect molecular knowledge to phenomena at the level of cells, organs and organisms. Recent studies suggest that students lack a framework to reason about

  5. Modelling Molecular Mechanisms: A Framework of Scientific Reasoning to Construct Molecular-Level Explanations for Cellular Behaviour

    Science.gov (United States)

    van Mil, Marc H. W.; Boerwinkel, Dirk Jan; Waarlo, Arend Jan

    2013-01-01

    Although molecular-level details are part of the upper-secondary biology curriculum in most countries, many studies report that students fail to connect molecular knowledge to phenomena at the level of cells, organs and organisms. Recent studies suggest that students lack a framework to reason about complex systems to make this connection. In this…

  6. Cellular and molecular research to reduce uncertainties in estimates of health effects from low-level radiation

    International Nuclear Information System (INIS)

    A study was undertaken by five radiation scientists to examine the feasibility of reducing the uncertainties in the estimation of risk due to protracted low doses of ionizing radiation. In addressing the question of feasibility, a review was made by the study group: of the cellular, molecular, and mammalian radiation data that are available; of the way in which altered oncogene properties could be involved in the loss of growth control that culminates in tumorigenesis; and of the progress that had been made in the genetic characterizations of several human and animal neoplasms. On the basis of this analysis, the study group concluded that, at the present time, it is feasible to mount a program of radiation research directed at the mechanism(s) of radiation-induced cancer with special reference to risk of neoplasia due to protracted, low doses of sparsely ionizing radiation. To implement a program of research, a review was made of the methods, techniques, and instruments that would be needed. This review was followed by a survey of the laboratories and institutions where scientific personnel and facilities are known to be available. A research agenda of the principal and broad objectives of the program is also discussed. 489 refs., 21 figs., 14 tabs

  7. Cellular and molecular research to reduce uncertainties in estimates of health effects from low-level radiation

    Energy Technology Data Exchange (ETDEWEB)

    Elkind, M.M.; Bedford, J.; Benjamin, S.A.; Waldren, C.A. (Colorado State Univ., Fort Collins, CO (USA)); Gotchy, R.L. (Science Applications International Corp., McLean, VA (USA))

    1990-10-01

    A study was undertaken by five radiation scientists to examine the feasibility of reducing the uncertainties in the estimation of risk due to protracted low doses of ionizing radiation. In addressing the question of feasibility, a review was made by the study group: of the cellular, molecular, and mammalian radiation data that are available; of the way in which altered oncogene properties could be involved in the loss of growth control that culminates in tumorigenesis; and of the progress that had been made in the genetic characterizations of several human and animal neoplasms. On the basis of this analysis, the study group concluded that, at the present time, it is feasible to mount a program of radiation research directed at the mechanism(s) of radiation-induced cancer with special reference to risk of neoplasia due to protracted, low doses of sparsely ionizing radiation. To implement a program of research, a review was made of the methods, techniques, and instruments that would be needed. This review was followed by a survey of the laboratories and institutions where scientific personnel and facilities are known to be available. A research agenda of the principal and broad objectives of the program is also discussed. 489 refs., 21 figs., 14 tabs.

  8. Elevated level of spindle checkprotein MAD2 correlates with cellular mitotic arrest, but not with aneuploidy and clinicopathological characteristics in gastric cancer

    Institute of Scientific and Technical Information of China (English)

    Chew-Wun Wu; Chin-Wen Chi; Tze-Sing Huang

    2004-01-01

    AIM: To study the relevance of spindle assembly checkprotein MAD2 to cellular mitotic status, aneuploidy and other clinicopathological characteristics in gastric cancer.METHODS: Western blot analyses were performed to analyze the protein levels of MAD2 and cyclin B1 in the tumorous and adjacent nontumorous tissues of 34 gastric cancer patients. Cell cycle distribution and DNA ploidy of cancer tissues were also determined by flow cytometry.Conventional statistical methods were adopted to determine the relevance of abnormal MAD2 level to mitotic status,aneuploidy and clinicopathological parameters.RESULTS: Out of 34 gastric cancer patients 25 (74%)exhibited elevated MAD2 levels in their tumorous tissues compared with the corresponding nontumorous tissues.Elevation of MAD2 levels significantly correlated with the increased levels of cydin B1 expression and G2/M-phase distribution (P = 0.038 and P = 0.033, respectively), but was not relevant to aneuploidy. The gastric cancer patients with elevated MAD2 levels showed a tendency toward better disease-free and overall survival (P>0.05). However, no association was found between elevated MAD2 levels and patients' clinicopathological characteristics.CONCLUSION: Elevation of MAD2 level is present in 74%of gastric cancer patients, and correlates with increased mitotic checkpoint activity. However, elevation of MAD2level is not associated with patients' aneuploidy and any of the clinicopathological characteristics.

  9. Asymptotic Behavior of Excitable Cellular Automata

    CERN Document Server

    Durrett, R; Durrett, Richard; Griffeath, David

    1993-01-01

    Abstract: We study two families of excitable cellular automata known as the Greenberg-Hastings Model (GHM) and the Cyclic Cellular Automaton (CCA). Each family consists of local deterministic oscillating lattice dynamics, with parallel discrete-time updating, parametrized by the range of interaction, the "shape" of its neighbor set, threshold value for contact updating, and number of possible states per site. GHM and CCA are mathematically tractable prototypes for the spatially distributed periodic wave activity of so-called excitable media observed in diverse disciplines of experimental science. Earlier work by Fisch, Gravner, and Griffeath studied the ergodic behavior of these excitable cellular automata on Z^2, and identified two distinct (but closely-related) elaborate phase portraits as the parameters vary. In particular, they noted the emergence of asymptotic phase diagrams (and Euclidean dynamics) in a well-defined threshold-range scaling limit. In this study we present several rigorous results and som...

  10. Application of Tomita-Takesaki theory in algebraic euclidean field theories

    CERN Document Server

    Schlingemann, D

    1999-01-01

    The construction of the known interacting quantum field theory models is mostly based on euclidean techniques. The expectation values of interesting quantities are usually given in terms of euclidean correlation functions from which one should be able to extract information about the behavior of the correlation functions of the Minkowskian counterpart. We think that the C*-algebraic approach to euclidean field theory gives an appropriate setup in order to study structural aspects model independently. A previous paper deals with a construction scheme which relates to each euclidean field theory a Poincaré covariant quantum field theory model in the sense of R. Haag and D. Kastler. Within the framework of R. Haag and D. Kastler, the physical concept of PCT symmetry and spin and statistics is related to the Tomita-Takesaki theory of von Neumann algebras and this important aspects has been studied by several authors. We express the PCT symmetry in terms of euclidean reflexions and we explicitly identify the corr...

  11. Epileptic Seizure Detection with Log-Euclidean Gaussian Kernel-Based Sparse Representation.

    Science.gov (United States)

    Yuan, Shasha; Zhou, Weidong; Wu, Qi; Zhang, Yanli

    2016-05-01

    Epileptic seizure detection plays an important role in the diagnosis of epilepsy and reducing the massive workload of reviewing electroencephalography (EEG) recordings. In this work, a novel algorithm is developed to detect seizures employing log-Euclidean Gaussian kernel-based sparse representation (SR) in long-term EEG recordings. Unlike the traditional SR for vector data in Euclidean space, the log-Euclidean Gaussian kernel-based SR framework is proposed for seizure detection in the space of the symmetric positive definite (SPD) matrices, which form a Riemannian manifold. Since the Riemannian manifold is nonlinear, the log-Euclidean Gaussian kernel function is applied to embed it into a reproducing kernel Hilbert space (RKHS) for performing SR. The EEG signals of all channels are divided into epochs and the SPD matrices representing EEG epochs are generated by covariance descriptors. Then, the testing samples are sparsely coded over the dictionary composed by training samples utilizing log-Euclidean Gaussian kernel-based SR. The classification of testing samples is achieved by computing the minimal reconstructed residuals. The proposed method is evaluated on the Freiburg EEG dataset of 21 patients and shows its notable performance on both epoch-based and event-based assessments. Moreover, this method handles multiple channels of EEG recordings synchronously which is more speedy and efficient than traditional seizure detection methods. PMID:26906674

  12. Epileptic Seizure Detection with Log-Euclidean Gaussian Kernel-Based Sparse Representation.

    Science.gov (United States)

    Yuan, Shasha; Zhou, Weidong; Wu, Qi; Zhang, Yanli

    2016-05-01

    Epileptic seizure detection plays an important role in the diagnosis of epilepsy and reducing the massive workload of reviewing electroencephalography (EEG) recordings. In this work, a novel algorithm is developed to detect seizures employing log-Euclidean Gaussian kernel-based sparse representation (SR) in long-term EEG recordings. Unlike the traditional SR for vector data in Euclidean space, the log-Euclidean Gaussian kernel-based SR framework is proposed for seizure detection in the space of the symmetric positive definite (SPD) matrices, which form a Riemannian manifold. Since the Riemannian manifold is nonlinear, the log-Euclidean Gaussian kernel function is applied to embed it into a reproducing kernel Hilbert space (RKHS) for performing SR. The EEG signals of all channels are divided into epochs and the SPD matrices representing EEG epochs are generated by covariance descriptors. Then, the testing samples are sparsely coded over the dictionary composed by training samples utilizing log-Euclidean Gaussian kernel-based SR. The classification of testing samples is achieved by computing the minimal reconstructed residuals. The proposed method is evaluated on the Freiburg EEG dataset of 21 patients and shows its notable performance on both epoch-based and event-based assessments. Moreover, this method handles multiple channels of EEG recordings synchronously which is more speedy and efficient than traditional seizure detection methods.

  13. Log-Euclidean metrics for fast and simple calculus on diffusion tensors.

    Science.gov (United States)

    Arsigny, Vincent; Fillard, Pierre; Pennec, Xavier; Ayache, Nicholas

    2006-08-01

    Diffusion tensor imaging (DT-MRI or DTI) is an emerging imaging modality whose importance has been growing considerably. However, the processing of this type of data (i.e., symmetric positive-definite matrices), called "tensors" here, has proved difficult in recent years. Usual Euclidean operations on matrices suffer from many defects on tensors, which have led to the use of many ad hoc methods. Recently, affine-invariant Riemannian metrics have been proposed as a rigorous and general framework in which these defects are corrected. These metrics have excellent theoretical properties and provide powerful processing tools, but also lead in practice to complex and slow algorithms. To remedy this limitation, a new family of Riemannian metrics called Log-Euclidean is proposed in this article. They also have excellent theoretical properties and yield similar results in practice, but with much simpler and faster computations. This new approach is based on a novel vector space structure for tensors. In this framework, Riemannian computations can be converted into Euclidean ones once tensors have been transformed into their matrix logarithms. Theoretical aspects are presented and the Euclidean, affine-invariant, and Log-Euclidean frameworks are compared experimentally. The comparison is carried out on interpolation and regularization tasks on synthetic and clinical 3D DTI data. PMID:16788917

  14. A Monte Carlo study of energy deposition at the sub-cellular level for application to targeted radionuclide therapy with low-energy electron emitters

    Energy Technology Data Exchange (ETDEWEB)

    Emfietzoglou, D. [Medical Physics Laboratory, University of Ioannina Medical School, Ioannina 451 10 (Greece); Bousis, C. [Medical Physics Laboratory, University of Ioannina Medical School, Ioannina 451 10 (Greece); Hindorf, C. [Joint Department of Physics, The Royal Marsden Hospital and Institute of Cancer Research, Sutton, Surrey SM2 5PT (United Kingdom); Fotopoulos, A. [Department of Nuclear Medicine, University of Ioannina Medical School, Ioannina 451 10 (Greece); Pathak, A. [School of Physics, University of Hyderabad, Hyderabad 500 046 (India); Kostarelos, K. [Nanomedicine Laboratory, Centre for Drug Delivery Research, School of Pharmacy, University of London, London WC1N 1AX (United Kingdom)]. E-mail: kostas.kostarelos@pharmacy.ac.uk

    2007-03-15

    Optimizing targeted radionuclide therapy for patients with circulating malignant cells (e.g. blood-related cancers) or a micrometastatic spread requires quantification of various dosimetric parameters at the single-cell level. We present results on the energy deposition of monoenergetic electrons of initial energy from 100 eV to 20 keV - relevant to Auger emitting radionuclides - distributed either uniformly or at the surface of spherical volumes of radii from 10 nm to 1 {mu}m which correspond to critical sub-cellular targets. Calculations have been carried out by our detailed-history Monte Carlo (MC) code which simulates event-by-event the complete slowing down (to 1 Ry) of both the primary and all subsequent generations of electrons, as well as, by the continuous-slowing-down-approximation (CSDA) using analytic range-energy relationships. The latter method has been adopted by the MIRD committee of the Society of Nuclear Medicine for dosimetry at the cellular level (>1 {mu}m). Differences between the MC and CSDA results are up to {approx}50% and are expected to be even larger at higher energies and/or smaller volumes. They are attributed to the deficiencies of the CSDA method associated with the neglect of straggling and {delta}-ray transport. The results are particularly relevant to targeted radiotherapy at the genome level by Auger emitters.

  15. Cellular automata

    CERN Document Server

    Codd, E F

    1968-01-01

    Cellular Automata presents the fundamental principles of homogeneous cellular systems. This book discusses the possibility of biochemical computers with self-reproducing capability.Organized into eight chapters, this book begins with an overview of some theorems dealing with conditions under which universal computation and construction can be exhibited in cellular spaces. This text then presents a design for a machine embedded in a cellular space or a machine that can compute all computable functions and construct a replica of itself in any accessible and sufficiently large region of t

  16. Toxicity of selenite in the unicellular green alga Chlamydomonas reinhardtii: Comparison between effects at the population and sub-cellular level

    International Nuclear Information System (INIS)

    The toxicity of selenium in aquatic ecosystems is mainly linked to its uptake and biotransformation by micro-organisms, and its subsequent transfer upwards into the food chain. Thus, organisms at low trophic level, such as algae, play a crucial role. The aim of our study was to investigate the biological effects of selenite on Chlamydomonas reinhardtii, both at the sub-cellular level (effect on ultrastructure) and at the population level (effect on growth). The cells were grown under batch culture conditions in well-defined media and exposed to waterborne selenite at concentrations up to 500 μM; i.e. up to lethal conditions. Based on the relationship between Se concentration and cell density achieved after a 96 h exposure period, an EC50 of 80 μM with a 95% confidence interval ranging between 64 and 98 μM was derived. No adaptation mechanisms were observed: the same toxicity was quantified for algae pre-contaminated with Se. The inhibition of growth was linked to impairments observed at the sub-cellular level. The intensity of the ultrastructural damages caused by selenite exposure depended on the level and duration of exposure. Observations by TEM suggested chloroplasts as the first target of selenite cytotoxicity, with effects on the stroma, thylakoids and pyrenoids. At higher concentrations, we could observe an increase in the number and volume of starch grains. For cells collected at 96 h, electron-dense granules were observed. Energy-dispersive X-ray microanalysis revealed that these granules contained selenium and were also rich in calcium and phosphorus. This study confirms that the direct toxicity of selenite on the phytoplankton biomass is not likely to take place at concentrations found in the environment. At higher concentrations, the link between effects at the sub-cellular and population levels, the over-accumulation of starch, and the formation of dense granules containing selenium are reported for the first time in the literature for a

  17. Special geometry of euclidean supersymmetry II. Hypermultiplets and the c-map

    Energy Technology Data Exchange (ETDEWEB)

    Cortes, Vicente [Institut de Mathematiques Elie Cartan, Universite Henri Poincare - Nancy I, B.P. 239, F-54506 Vandoeuvre-les-Nancy (France); Mayer, Christoph [Theoretisch-Physikalisches Institut, Friedrich-Schiller-Universitaet Jena, Max-Wien-Platz 1, D-07743 Jena (Germany); Mohaupt, Thomas [Theoretisch-Physikalisches Institut, Friedrich-Schiller-Universitaet Jena, Max-Wien-Platz 1, D-07743 Jena (Germany); Saueressig, Frank [Institute for Theoretical Physics and Spinoza Institute, Postbus 80.195, 3508 TD Utrecht (Netherlands)

    2005-06-01

    We construct two new versions of the c-map which allow us to obtain the target manifolds of hypermultiplets in euclidean theories with rigid N = 2 supersymmetry. While the minkowskian para-c-map is obtained by dimensional reduction of the minkowskian vector multiplet lagrangian over time, the euclidean para-c-map corresponds to the dimensional reduction of the euclidean vector multiplet lagrangian. In both cases the resulting hypermultiplet target spaces are para-hyper-Kaehler manifolds. We review and prove the relevant results of para-complex and para-hypercomplex geometry. In particular, we give a second, purely geometrical construction of both c-maps, by proving that the cotangent bundle N = T*M of any affine special (para-)Kaehler manifold M is para-hyper-Kaehler.

  18. Linear time constant factor approximation algorithm for the Euclidean ``Freeze-Tag`` robot awakening problem

    Directory of Open Access Journals (Sweden)

    Mohammad Javad Namazifar

    2015-09-01

    Full Text Available The Freeze-Tag Problem (FTP arises in the study of swarm robotics. The FTP is a combinatorial optimization problem that starts by locating a set of robots in a Euclidean plane. Here, we are given a swarm of n asleep (frozen or inactive robots and a single awake (active robot. In order to activate an inactive robot in FTP, the active robot should either be in the physical proximity to the inactive robot or ``touch`` it. The new activated robot starts moving and can wake up other inactive robots. The goal is to find an optimal activating schedule with the minimum time required for activating all robots. In general, FTP is an NP-Hard problem and in the Euclidean space is an open problem. In this paper, we present a recursive approximation algorithm with a constant approximation factor and a linear running time for the Euclidean Freeze-Tag Problem.

  19. Molecular Mechanistic Reasoning: Toward Bridging the Gap between the Molecular and Cellular Levels in Life Science Education

    Science.gov (United States)

    van Mil, Marc H. W.; Postma, Paulien A.; Boerwinkel, Dirk Jan; Klaassen, Kees; Waarlo, Arend Jan

    2016-01-01

    Although learning about DNA, RNA, and proteins is part of the upper secondary biology curriculum in most countries, many studies report that students fail to connect molecular knowledge to phenomena at the higher level of cells, organs, and organisms. As a result, many students use memorization and rote learning as a coping strategy when presented…

  20. Ethacrynic acid inhibition of histamine release from rat mast cells: effect on cellular ATP levels and thiol groups

    DEFF Research Database (Denmark)

    Johansen, Torben

    1983-01-01

    The experiments concerned the effect of ethacrynic acid (0.5 mM) on the adenosine triphosphate (ATP) content of rat mast cells and the effect on histamine release induced by the ionophore A23187 (10 microM). Ethacrynic acid decreased the ATP level of the cells in presence of antimycin A and glucose...

  1. On the Equivalence between Euclidean and In-In Formalisms in de Sitter QFT

    OpenAIRE

    HIGUCHI, Atsushi; Marolf, Donald; Morrison, Ian A.

    2010-01-01

    We study the relation between two sets of correlators in interacting quantum field theory on de Sitter space. The first are correlators computed using in-in perturbation theory in the expanding cosmological patch of de Sitter space (also known as the conformal patch, or the Poincar\\'e patch), and for which the free propagators are taken to be those of the free Euclidean vacuum. The second are correlators obtained by analytic continuation from Euclidean de Sitter; i.e., they are correlators in...

  2. On the local structure and the homology of CAT$(\\kappa)$ spaces and euclidean buildings

    OpenAIRE

    Kramer, Linus

    2010-01-01

    We prove that every open subset of a euclidean building is a finite dimensional absolute neighborhood retract. This implies in particular that such a set has the homotopy type of a finite dimensional simplicial complex. We also include a proof for the rigidity of homeomorphisms of euclidean buildings. A key step in our approach to this result is the following: the space of directions $\\Sigma_oX$ of a CAT$(\\kappa)$ space $X$ is homotopy quivalent to a small punctured disk $B_\\eps(X,o)\\setminus...

  3. Correlation function for the Grid-Poisson Euclidean matching on a line and on a circle

    International Nuclear Information System (INIS)

    We compute the two-point correlation function for spin configurations which are obtained by solving the Euclidean matching problem, for one family of points on a grid and the second family chosen uniformly at random, when the cost depends on a power p of the Euclidean distance. We provide the analytic solution in the thermodynamic limit, in a number of cases (p > 1 open b.c. and p = 2 periodic b.c., both at criticality) and analyse numerically other parts of the phase diagram. (paper)

  4. Statistical 2D and 3D shape analysis using Non-Euclidean Metrics

    DEFF Research Database (Denmark)

    Larsen, Rasmus; Hilger, Klaus Baggesen; Wrobel, Mark Christoph

    2002-01-01

    We address the problem of extracting meaningful, uncorrelated biological modes of variation from tangent space shape coordinates in 2D and 3D using non-Euclidean metrics. We adapt the maximum autocorrelation factor analysis and the minimum noise fraction transform to shape decomposition. Furtherm......We address the problem of extracting meaningful, uncorrelated biological modes of variation from tangent space shape coordinates in 2D and 3D using non-Euclidean metrics. We adapt the maximum autocorrelation factor analysis and the minimum noise fraction transform to shape decomposition...

  5. Solutions manual to accompany Classical geometry Euclidean, transformational, inversive, and projective

    CERN Document Server

    Leonard, I E; Liu, A C F; Tokarsky, G W

    2014-01-01

    Solutions Manual to accompany Classical Geometry: Euclidean, Transformational, Inversive, and Projective Written by well-known mathematical problem solvers, Classical Geometry: Euclidean, Transformational, Inversive, and Projective features up-to-date and applicable coverage of the wide spectrum of geometry and aids readers in learning the art of logical reasoning, modeling, and proof. With its reader-friendly approach, this undergraduate text features self-contained topical coverage and provides a large selection of solved exercises to aid in reader comprehension. Material in this text can be tailored for a one-, two-, or three-semester sequence.

  6. Analyzing the Expression Level and Cellular Location of the Tip-1 Protein in Oral Cancer Cell Lines

    Directory of Open Access Journals (Sweden)

    N Mansoursamaei

    2005-10-01

    Full Text Available Oral squamous cell carcinoma (OSCC is the sixth most common cancer in the world and accounts for approximately 4% of all cancers and 2% of all cancer death.The single most important factor in the prevention of the disease is early detection although, due to increased risk of secondary malignancy, survival remains poor with only a 25% 5 years survival. Both hereditary and environmental factors have been shown to have a productive role in this disease. For example, although chronic exposure of oral epithelium to tobacco smoke and alcohol are amongst the most important aetiological factors, it is now becoming realized that infection with high risk types of human papilloma virus (HPV are also involved as causative agent in a subset of this disease. All of these OSCC associated factors are known to promote genetic instability in the target oral epithelial cells. Work in our laboratories has indicated that the Tax interacting protein 1 (Tip-1 is also a target for the HPV 16 E6 protein may play an important role in controlling genetic instability during the oncogenic process (Hampson L., et al unpublished. So far 14 oral cancer cell lines have been grown in cell culture and RNA extracted from these. Tip-1 transcript levels were analyzed in this material by Northern blotting and competitive template quantitative PCR, which showed that Tip-1 levels were higher in some, cell lines than others (4 high, 6 moderate, 4 low level. Cell ploidy was determined by FACS analysis of propidium iodide stained cells, which showed that out of all the OSCC cell lines tested the cell line (BICR 68 had the greatest numbers of polyploid cells and also had the highest expression of Tip-1 RNA.

  7. Towards a Cellular Automata Based Network Intrusion Detection System with Power Level Metric in Wireless Adhoc Networks (IDFADNWCA)

    OpenAIRE

    Sree, Pokkuluri Kiran; Babu, Inampudi Ramesh

    2014-01-01

    Adhoc wireless network with their changing topology and distributed nature are more prone to intruders. The efficiency of an Intrusion detection system in the case of an adhoc network is not only determined by its dynamicity in monitoring but also in its flexibility in utilizing the available power in each of its nodes. In this paper we propose a hybrid intrusion detection system, based on a power level metric for potential adhoc hosts, which is used to determine the duration for which a part...

  8. Magnetic nanoparticle hyperthermia cancer treatment efficacy dependence on cellular and tissue level particle concentration and particle heating properties

    Science.gov (United States)

    Petryk, Alicia A.; Misra, Adwiteeya; Mazur, Courtney M.; Petryk, James D.; Hoopes, P. J.

    2015-03-01

    The use of nanotechnology for the treatment of cancer affords the possibility of highly specific tumor targeting and improved treatment efficacy. Iron oxide magnetic nanoparticles (IONPs) have demonstrated success as an ablative mono-therapy and targetable adjuvant therapy. However, the relative therapeutic value of intracellular vs. extracellular IONPs remains unclear. Our research demonstrates that both extracellular and intracellular IONPs generate cytotoxicity when excited by an alternating magnetic field (AMF). While killing individual cells via intracellular IONP heating is an attractive goal, theoretical models and experimental results suggest that this may not be possible due to limitations of cell volume, applied AMF, IONP concentration and specific absorption rate (SAR). The goal of this study was to examine the importance of tumor size (cell number) with respect to IONP concentration. Mouse mammary adenocarcinoma cells were incubated with IONPs, washed, spun into different pellet sizes (0.1, 0.5 and 2 million cells) and exposed to AMF. The level of heating and associated cytotoxicity depended primarily on the number of IONPs /amount Fe per cell pellet volume and the relative volume of the cell pellet. Specifically, larger cell pellets achieved greater relative cytotoxicity due to greater iron amounts, close association and subsequently higher temperatures.

  9. Towards instantaneous cellular level bio diagnosis: laser extraction and imaging of biological entities with conserved integrity and activity.

    Science.gov (United States)

    Ren, L; Robertson, W D; Reimer, R; Heinze, C; Schneider, C; Eggert, D; Truschow, P; Hansen, N-O; Kroetz, P; Zou, J; Miller, R J D

    2015-07-17

    The prospect for spatial imaging with mass spectroscopy at the level of the cell requires new means of cell extraction to conserve molecular structure. To this aim, we demonstrate a new laser extraction process capable of extracting intact biological entities with conserved biological function. The method is based on the recently developed picosecond infrared laser (PIRL), designed specifically to provide matrix-free extraction by selectively exciting the water vibrational modes under the condition of ultrafast desorption by impulsive vibrational excitation (DIVE). The basic concept is to extract the constituent protein structures on the fastest impulsive limit for ablation to avoid excessive thermal heating of the proteins and to use strongly resonant 1-photon conditions to avoid multiphoton ionization and degradation of the sample integrity. With various microscope imaging and biochemical analysis methods, nanoscale single protein molecules, viruses, and cells in the ablation plume are found to be morphologically and functionally identical with their corresponding controls. This method provides a new means to resolve chemical activity within cells and is amenable to subcellular imaging with near-field approaches. The most important finding is the conserved nature of the extracted biological material within the laser ablation plume, which is fully consistent with in vivo structures and characteristics. PMID:26111866

  10. The glycolytic shift in fumarate-hydratase-deficient kidney cancer lowers AMPK levels, increases anabolic propensities and lowers cellular iron levels

    KAUST Repository

    Tong, Winghang

    2011-09-01

    Inactivation of the TCA cycle enzyme, fumarate hydratase (FH), drives a metabolic shift to aerobic glycolysis in FH-deficient kidney tumors and cell lines from patients with hereditary leiomyomatosis renal cell cancer (HLRCC), resulting in decreased levels of AMP-activated kinase (AMPK) and p53 tumor suppressor, and activation of the anabolic factors, acetyl-CoA carboxylase and ribosomal protein S6. Reduced AMPK levels lead to diminished expression of the DMT1 iron transporter, and the resulting cytosolic iron deficiency activates the iron regulatory proteins, IRP1 and IRP2, and increases expression of the hypoxia inducible factor HIF-1α, but not HIF-2α. Silencing of HIF-1α or activation of AMPK diminishes invasive activities, indicating that alterations of HIF-1α and AMPK contribute to the oncogenic growth of FH-deficient cells. © 2011 Elsevier Inc.

  11. mRNA expression levels in failing human hearts predict cellular electrophysiological remodeling: a population-based simulation study.

    Directory of Open Access Journals (Sweden)

    John Walmsley

    Full Text Available Differences in mRNA expression levels have been observed in failing versus non-failing human hearts for several membrane channel proteins and accessory subunits. These differences may play a causal role in electrophysiological changes observed in human heart failure and atrial fibrillation, such as action potential (AP prolongation, increased AP triangulation, decreased intracellular calcium transient (CaT magnitude and decreased CaT triangulation. Our goal is to investigate whether the information contained in mRNA measurements can be used to predict cardiac electrophysiological remodeling in heart failure using computational modeling. Using mRNA data recently obtained from failing and non-failing human hearts, we construct failing and non-failing cell populations incorporating natural variability and up/down regulation of channel conductivities. Six biomarkers are calculated for each cell in each population, at cycle lengths between 1500 ms and 300 ms. Regression analysis is performed to determine which ion channels drive biomarker variability in failing versus non-failing cardiomyocytes. Our models suggest that reported mRNA expression changes are consistent with AP prolongation, increased AP triangulation, increased CaT duration, decreased CaT triangulation and amplitude, and increased delay between AP and CaT upstrokes in the failing population. Regression analysis reveals that changes in AP biomarkers are driven primarily by reduction in I[Formula: see text], and changes in CaT biomarkers are driven predominantly by reduction in I(Kr and SERCA. In particular, the role of I(CaL is pacing rate dependent. Additionally, alternans developed at fast pacing rates for both failing and non-failing cardiomyocytes, but the underlying mechanisms are different in control and heart failure.

  12. Fast Exact Euclidean Distance (FEED) : A new class of adaptable distance transforms

    NARCIS (Netherlands)

    Schouten, Theo E.; van den Broek, Egon L.

    2014-01-01

    A new unique class of foldable distance transforms of digital images (DT) is introduced, baptized: Fast Exact Euclidean Distance (FEED) transforms. FEED class algorithms calculate the DT startingdirectly from the definition or rather its inverse. The principle of FEED class algorithms is introduced,

  13. Fast Exact Euclidean Distance (FEED): A new class of adaptable distance transforms

    NARCIS (Netherlands)

    Schouten, Theo E.; Broek, van den Egon L.

    2014-01-01

    A new unique class of foldable distance transforms of digital images (DT) is introduced, baptized: Fast Exact Euclidean Distance (FEED) transforms. FEED class algorithms calculate the DT starting directly from the definition or rather its inverse. The principle of FEED class algorithms is introduced

  14. Special Geometry of Euclidean Supersymmetry IV: the local c-map

    CERN Document Server

    Cortés, Vicente; Mohaupt, Thomas; Vaughan, Owen

    2015-01-01

    We consider timelike and spacelike reductions of 4D, N = 2 Minkowskian and Euclidean vector multiplets coupled to supergravity and the maps induced on the scalar geometry. In particular, we investigate (i) the (standard) spatial c-map, (ii) the temporal c-map, which corresponds to the reduction of the Minkowskian theory over time, and (iii) the Euclidean c-map, which corresponds to the reduction of the Euclidean theory over space. In the last two cases we prove that the target manifold is para-quaternionic Kahler. In cases (i) and (ii) we construct two integrable complex structures on the target manifold, one of which belongs to the quaternionic and para-quaternionic structure, respectively. In case (iii) we construct two integrable para-complex structures, one of which belongs to the para-quaternionic structure. In addition we provide a new global construction of the spatial, temporal and Euclidean c-maps, and separately consider a description of the target manifold as a fibre bundle over a projective specia...

  15. Faster exact algorithms for computing Steiner trees in higher dimensional Euclidean spaces

    DEFF Research Database (Denmark)

    Fonseca, Rasmus; Brazil, Marcus; Winter, Pawel;

    2016-01-01

    The Euclidean Steiner tree problem asks for a network of minimum total length interconnecting a finite set of points in d-dimensional space. For d ≥ 3, only one practical algorithmic approach exists for this problem --- proposed by Smith in 1992. A number of refinements of Smith's algorithm have ...

  16. Ab-initio reconstruction of complex Euclidean networks in two dimensions

    Science.gov (United States)

    Gujarathi, S. R.; Farrow, C. L.; Glosser, C.; Granlund, L.; Duxbury, P. M.

    2014-05-01

    Reconstruction of complex structures is an inverse problem arising in virtually all areas of science and technology, from protein structure determination to bulk heterostructure solar cells and the structure of nanoparticles. We cast this problem as a complex network problem where the edges in a network have weights equal to the Euclidean distance between their endpoints. We present a method for reconstruction of the locations of the nodes of the network given only the edge weights of the Euclidean network. The theoretical foundations of the method are based on rigidity theory, which enables derivation of a polynomial bound on its efficiency. An efficient implementation of the method is discussed and timing results indicate that the run time of the algorithm is polynomial in the number of nodes in the network. We have reconstructed Euclidean networks of about 1000 nodes in approximately 24 h on a desktop computer using this implementation. We also reconstruct Euclidean networks corresponding to polymer chains in two dimensions and planar graphene nanoparticles. We have also modified our base algorithm so that it can successfully solve random point sets when the input data are less precise.

  17. Short-scale Emergence of Classical Geometry, in Euclidean Loop Quantum Gravity

    CERN Document Server

    Bayle, Vincent; Rovelli, Carlo

    2016-01-01

    We study the euclidean covariant loop-quantum-gravity vertex numerically, using a cylindrically symmetric boundary state and a convenient value of the Barbero-Immirzi parameter. We show that a classical geometry emerges already at low spin. We also recognise the appearance of the degenerate configurations.

  18. Mayer expansions for Euclidean lattice field theory: Convergence properties and relation with perturbation theory

    International Nuclear Information System (INIS)

    The author describes the Mayer expansion in Euclidean lattice field theory by comparing it with the statistical mechanics of polymer systems. In this connection he discusses the Borel summability and the analyticity of the activities on the lattice. Furthermore the relations between renormalization and the Mayer expansion are considered. (HSI)

  19. Usability Evaluation of an Augmented Reality System for Teaching Euclidean Vectors

    Science.gov (United States)

    Martin-Gonzalez, Anabel; Chi-Poot, Angel; Uc-Cetina, Victor

    2016-01-01

    Augmented reality (AR) is one of the emerging technologies that has demonstrated to be an efficient technological tool to enhance learning techniques. In this paper, we describe the development and evaluation of an AR system for teaching Euclidean vectors in physics and mathematics. The goal of this pedagogical tool is to facilitate user's…

  20. On the Equivalence of the Berlekamp-Massey and the Euclidean Algorithm for Decoding

    DEFF Research Database (Denmark)

    Heydtmann, Agnes Eileen; Jensen, Jørn Møller

    1999-01-01

    The Berlekamp-Massey algorithm and the Euclidean algorithm for decoding have been considered as two different algorithms for solving the same problem, namely the one given by the key equation. In this article we argue that they are essentially identical by showing how one can be adapted to perform...

  1. Euclidean skeletons of 3D data sets in linear time by the integer medial axis transform

    NARCIS (Netherlands)

    Hesselink, Wim H.; Visser, Menno; Roerdink, Jos B.T.M.; Ronse, C; Najman, L; Decenciere, E

    2005-01-01

    A general algorithm for computing Euclidean skeletons of 3D data sets in linear time is presented. These skeletons are defined in terms of a new concept, called the integer medial axis (IMA) transform. The algorithm is based upon the computation of 3D feature transforms, using a modification of an a

  2. Thinking Outside the Euclidean Box: Riemannian Geometry and Inter-Temporal Decision-Making.

    Science.gov (United States)

    Mishra, Himanshu; Mishra, Arul

    2016-01-01

    Inter-temporal decisions involves assigning values to various payoffs occurring at different temporal distances. Past research has used different approaches to study these decisions made by humans and animals. For instance, considering that people discount future payoffs at a constant rate (e.g., exponential discounting) or at variable rate (e.g., hyperbolic discounting). In this research, we question the widely assumed, but seldom questioned, notion across many of the existing approaches that the decision space, where the decision-maker perceives time and monetary payoffs, is a Euclidean space. By relaxing the rigid assumption of Euclidean space, we propose that the decision space is a more flexible Riemannian space of Constant Negative Curvature. We test our proposal by deriving a discount function, which uses the distance in the Negative Curvature space instead of Euclidean temporal distance. The distance function includes both perceived values of time as well as money, unlike past work which has considered just time. By doing so we are able to explain many of the empirical findings in inter-temporal decision-making literature. We provide converging evidence for our proposal by estimating the curvature of the decision space utilizing manifold learning algorithm and showing that the characteristics (i.e., metric properties) of the decision space resembles those of the Negative Curvature space rather than the Euclidean space. We conclude by presenting new theoretical predictions derived from our proposal and implications for how non-normative behavior is defined.

  3. Ab-initio reconstruction of complex Euclidean networks in two dimensions.

    Science.gov (United States)

    Gujarathi, S R; Farrow, C L; Glosser, C; Granlund, L; Duxbury, P M

    2014-05-01

    Reconstruction of complex structures is an inverse problem arising in virtually all areas of science and technology, from protein structure determination to bulk heterostructure solar cells and the structure of nanoparticles. We cast this problem as a complex network problem where the edges in a network have weights equal to the Euclidean distance between their endpoints. We present a method for reconstruction of the locations of the nodes of the network given only the edge weights of the Euclidean network. The theoretical foundations of the method are based on rigidity theory, which enables derivation of a polynomial bound on its efficiency. An efficient implementation of the method is discussed and timing results indicate that the run time of the algorithm is polynomial in the number of nodes in the network. We have reconstructed Euclidean networks of about 1000 nodes in approximately 24 h on a desktop computer using this implementation. We also reconstruct Euclidean networks corresponding to polymer chains in two dimensions and planar graphene nanoparticles. We have also modified our base algorithm so that it can successfully solve random point sets when the input data are less precise.

  4. A Quasi-Nonmetric Method for Multidimensional Scaling via an Extended Euclidean Model.

    Science.gov (United States)

    Winsberg, Suzanne; Carroll, J. Douglas

    1989-01-01

    An Extended Two-Way Euclidean Multidimensional Scaling (MDS) model that assumes both common and specific dimensions is described and contrasted with the "standard" (Two-Way) MDS model. Illustrations with both artificial and real data on the judged similarity of nations are provided. (TJH)

  5. On the sequences ri, si, ti ∈ ℤ related to extended Euclidean algorithm and continued fractions

    Science.gov (United States)

    Muhammad, Khairun Nisak; Kamarulhaili, Hailiza

    2016-06-01

    The extended Euclidean Algorithm is a practical technique used in many cryptographic applications, where it computes the sequences ri, si, ti ∈ ℤ that always satisfy ri = si a+ tib. The integer ri is the remainder in the ith sequences. The sequences si and ti arising from the extended Euclidean algorithm are equal, up to sign, to the convergents of the continued fraction expansion of a/b. The values of (ri, si, ti) satisfy various properties which are used to solve the shortest vector problem in representing point multiplications in elliptic curves cryptography, namely the GLV (Gallant, Lambert & Vanstone) integer decomposition method and the ISD (integer sub decomposition) method. This paper is to extend the proof for each of the existing properties on (ri, si, ti). We also generate new properties which are relevant to the sequences ri, si, ti ∈ ℤ. The concepts of Euclidean algorithm, extended Euclidean algorithm and continued fractions are intertwined and the properties related to these concepts are proved. These properties together with the existing properties of the sequence (ri, si, ti) are regarded as part and parcel of the building blocks of a new generation of an efficient cryptographic protocol.

  6. METHODOLOGICAL NOTES: Permutation asymmetry of the relativistic velocity addition law and non-Euclidean geometry

    Science.gov (United States)

    Ritus, V. I.

    2008-07-01

    The asymmetry of the relativistic addition law for noncollinear velocities under the velocity permutation leads to two modified triangles on a Euclidean plane depicting the addition of unpermuted and permuted velocities and the appearance of a nonzero angle ω between two resulting velocities. A particle spin rotates through the same angle ω under a Lorentz boost with a velocity noncollinear to the particle velocity. Three mutually connected three-parameter representations of the angle ω, obtained by the author earlier, express the three-parameter symmetry of the sides and angles of two Euclidean triangles identical to the sine and cosine theorems for the sides and angles of a single geodesic triangle on the surface of a pseudosphere. Namely, all three representations of the angle ω, after a transformation of one of them, coincide with the representations of the area of a pseudospherical triangle expressed in terms of any two of its sides and the angle between them. The angle ω is also symmetrically expressed in terms of three angles or three sides of a geodesic triangle, and therefore it is an invariant of the group of triangle motions over the pseudo-sphere surface, the group that includes the Lorentz group. Although the pseudospheres in Euclidean and pseudo-Euclidean spaces are locally isometric, only the latter is isometric to the entire Lobachevsky plane and forms a homogeneous isotropic curved 4-velocity space in the flat Minkowski space. In this connection, relativistic physical processes that may be related to the pseudosphere in Euclidean space are especially interesting.

  7. Distinct genetic loci control plasma HIV-RNA and cellular HIV-DNA levels in HIV-1 infection: the ANRS Genome Wide Association 01 study.

    Directory of Open Access Journals (Sweden)

    Cyril Dalmasso

    Full Text Available Previous studies of the HIV-1 disease have shown that HLA and Chemokine receptor genetic variants influence disease progression and early viral load. We performed a Genome Wide Association study in a cohort of 605 HIV-1-infected seroconverters for detection of novel genetic factors that influence plasma HIV-RNA and cellular HIV-DNA levels. Most of the SNPs strongly associated with HIV-RNA levels were localised in the 6p21 major histocompatibility complex (MHC region and were in the vicinity of class I and III genes. Moreover, protective alleles for four disease-associated SNPs in the MHC locus (rs2395029, rs13199524, rs12198173 and rs3093662 were strikingly over-represented among forty-five Long Term HIV controllers. Furthermore, we show that the HIV-DNA levels (reflecting the HIV reservoir are associated with the same four SNPs, but also with two additional SNPs on chromosome 17 (rs6503919; intergenic region flanked by the DDX40 and YPEL2 genes and chromosome 8 (rs2575735; within the Syndecan 2 gene. Our data provide evidence that the MHC controls both HIV replication and HIV reservoir. They also indicate that two additional genomic loci may influence the HIV reservoir.

  8. Euclidean-Minkowskian duality of Wilson-loop correlation functions

    OpenAIRE

    Giordano, M; Meggiolaro, E.

    2009-01-01

    We discuss the analyticity properties of the Wilson--loop correlation functions relevant to the problem of soft high-energy scattering, directly at the level of the functional integral, in a genuinely nonperturbative way.

  9. 电磁辐射的细胞生物学效应研究进展%Progress in biological effects on the cellular level of electromagnetic radiation

    Institute of Scientific and Technical Information of China (English)

    丁真; 李劲涛; 吴水才; 曾毅

    2015-01-01

    随着工业、信息行业的飞速发展,各种电子产品在给人类日常生活带来巨大便利的同时,产生的电磁污染也引起了人们的广泛关注。已有大量研究表明电磁辐射能从神经系统、免疫系统、生殖系统以及心脑血管系统等各个方面对人体产生不良影响,但具体微观作用机制还不能确定。本文综述了电磁辐射对细胞增殖、凋亡、细胞膜、受体分子、跨膜信号转导以及基因表达等方面的影响,详细介绍了细胞水平上电磁辐射的生物学效应,以期进一步对电磁辐射生物效应机制进行详细深入的研究。%With the rapid development of communication industry, the man⁃made electromagnetic pollution which was mainly caused by convenient electronic appliances has been a worldwide concern� Studies indicated that electromagnetic radiation ( EMR) could be harmful to human health, by affecting the nervous system, immune system, genital system and cardiocerebral vascular system� However, the cellular and molecular mechanisms were still unclear� The EMR impacts on cell viability, apoptosis, cell membrane function, cell receptor, cell signal transduction and genetic expression were illustrated and discussed here� The biological effects on the cellular level caused by EMR were summarized to provide evidences for further study on biological mechanism of EMR.

  10. Protein Secondary Structures (α-helix and β-sheet) at a Cellular Level and Protein Fractions in Relation to Rumen Degradation Behaviours of Protein: A New Approach

    International Nuclear Information System (INIS)

    Studying the secondary structure of proteins leads to an understanding of the components that make up a whole protein, and such an understanding of the structure of the whole protein is often vital to understanding its digestive behaviour and nutritive value in animals. The main protein secondary structures are the α-helix and β-sheet. The percentage of these two structures in protein secondary structures influences protein nutritive value, quality and digestive behaviour. A high percentage of β-sheet structure may partly cause a low access to gastrointestinal digestive enzymes, which results in a low protein value. The objectives of the present study were to use advanced synchrotron-based Fourier transform IR (S-FTIR) microspectroscopy as a new approach to reveal the molecular chemistry of the protein secondary structures of feed tissues affected by heat-processing within intact tissue at a cellular level, and to quantify protein secondary structures using multicomponent peak modelling Gaussian and Lorentzian methods, in relation to protein digestive behaviours and nutritive value in the rumen, which was determined using the Cornell Net Carbohydrate Protein System. The synchrotron-based molecular chemistry research experiment was performed at the National Synchrotron Light Source at Brookhaven National Laboratory, US Department of Energy. The results showed that, with S-FTIR microspectroscopy, the molecular chemistry, ultrastructural chemical make-up and nutritive characteristics could be revealed at a high ultraspatial resolution (∼10 μm). S-FTIR microspectroscopy revealed that the secondary structure of protein differed between raw and roasted golden flaxseeds in terms of the percentages and ratio of α-helixes and β-sheets in the mid-IR range at the cellular level. By using multicomponent peak modelling, the results show that the roasting reduced (P <0.05) the percentage of α-helixes (from 47.1% to 36.1%: S-FTIR absorption intensity), increased the

  11. Cellular Telephone

    Institute of Scientific and Technical Information of China (English)

    杨周

    1996-01-01

    Cellular phones, used in automobiles, airliners, and passenger trains, are basically low-power radiotelephones. Calls go through radio transmitters that are located within small geographical units called cells. Because each cell’s signals are too weak to interfere with those of other cells operating on the same fre-

  12. Soybean extracts increase cell surface ZIP4 abundance and cellular zinc levels: a potential novel strategy to enhance zinc absorption by ZIP4 targeting.

    Science.gov (United States)

    Hashimoto, Ayako; Ohkura, Katsuma; Takahashi, Masakazu; Kizu, Kumiko; Narita, Hiroshi; Enomoto, Shuichi; Miyamae, Yusaku; Masuda, Seiji; Nagao, Masaya; Irie, Kazuhiro; Ohigashi, Hajime; Andrews, Glen K; Kambe, Taiho

    2015-12-01

    Dietary zinc deficiency puts human health at risk, so we explored strategies for enhancing zinc absorption. In the small intestine, the zinc transporter ZIP4 functions as an essential component of zinc absorption. Overexpression of ZIP4 protein increases zinc uptake and thereby cellular zinc levels, suggesting that food components with the ability to increase ZIP4 could potentially enhance zinc absorption via the intestine. In the present study, we used mouse Hepa cells, which regulate mouse Zip4 (mZip4) in a manner indistinguishable from that in intestinal enterocytes, to screen for suitable food components that can increase the abundance of ZIP4. Using this ZIP4-targeting strategy, two such soybean extracts were identified that were specifically able to decrease mZip4 endocytosis in response to zinc. These soybean extracts also effectively increased the abundance of apically localized mZip4 in transfected polarized Caco2 and Madin-Darby canine kidney cells and, moreover, two apically localized mZip4 acrodermatitis enteropathica mutants. Soybean components were purified from one extract and soyasaponin Bb was identified as an active component that increased both mZip4 protein abundance and zinc levels in Hepa cells. Finally, we confirmed that soyasaponin Bb is capable of enhancing cell surface endogenous human ZIP4 in human cells. Our results suggest that ZIP4 targeting may represent a new strategy to improve zinc absorption in humans.

  13. Membrane paradigm and entropy of black holes in the Euclidean action approach

    CERN Document Server

    Lemos, José P S

    2011-01-01

    The membrane paradigm approach to black holes fixes in the vicinity of the event horizon a fictitious surface, the stretched horizon, so that the spacetime outside remains unchanged and the spacetime inside is vacuum. Using this powerful method, several black hole properties have been found and settled, such as the horizon's viscosity, electrical conductivity, resistivity, as well as other properties. On the other hand the Euclidean action approach to black hole spacetimes has been very fruitful in understanding black hole entropy. Combining both the Euclidean action and membrane paradigm approaches a direct derivation of the black hole entropy is given. In the derivation it is considered that the only fields present are the gravitational and matter fields, with no electric field.

  14. A Tale of Two Spaces: BPS Wilson Loops in Minkowski Spacetime and Euclidean Space

    CERN Document Server

    Ouyang, Hao; Zhang, Jia-ju

    2015-01-01

    We discuss BPS Wilson loops of $d=4$ $\\mathcal N=4$ super Yang-Mills theory, $d=3$ $\\mathcal N=2$ super Chern-Simons-matter theory, and $d=3$ $\\mathcal N=6$ Aharony-Bergman-Jafferis-Maldacena theory, in both Minkowski spacetime and Euclidean space. We find that there are timelike and null BPS Wilson loops in Minkowski spacetime, but spacelike BPS Wilson loops do not exist. We attribute this to the conflicts of reality conditions of spinors. However, in Euclidean space spacelike Wilson loops do exist. There are both Wilson lines of infinite straight lines and Wilson loops of circles. This is because the reality conditions of spinors are lost after Wick rotation. The result is reasonable in view of AdS/CFT correspondence.

  15. A Low-Complexity Euclidean Orthogonal LDPC Architecture for Low Power Applications.

    Science.gov (United States)

    Revathy, M; Saravanan, R

    2015-01-01

    Low-density parity-check (LDPC) codes have been implemented in latest digital video broadcasting, broadband wireless access (WiMax), and fourth generation of wireless standards. In this paper, we have proposed a high efficient low-density parity-check code (LDPC) decoder architecture for low power applications. This study also considers the design and analysis of check node and variable node units and Euclidean orthogonal generator in LDPC decoder architecture. The Euclidean orthogonal generator is used to reduce the error rate of the proposed LDPC architecture, which can be incorporated between check and variable node architecture. This proposed decoder design is synthesized on Xilinx 9.2i platform and simulated using Modelsim, which is targeted to 45 nm devices. Synthesis report proves that the proposed architecture greatly reduces the power consumption and hardware utilizations on comparing with different conventional architectures.

  16. Spatially adaptive log-euclidean polyaffine registration based on sparse matches.

    Science.gov (United States)

    Taquet, Maxime; Macq, Benoît; Warfield, Simon K

    2011-01-01

    Log-euclidean polyaffine transforms have recently been introduced to characterize the local affine behavior of the deformation in principal anatomical structures. The elegant mathematical framework makes them a powerful tool for image registration. However, their application is limited to large structures since they require the pre-definition of affine regions. This paper extends the polyaffine registration to adaptively fit a log-euclidean polyaffine transform that captures deformations at smaller scales. The approach is based on the sparse selection of matching points in the images and the formulation of the problem as an expectation maximization iterative closest point problem. The efficiency of the algorithm is shown through experiments on inter-subject registration of brain MRI between a healthy subject and patients with multiple sclerosis.

  17. Efficient tracker based on sparse coding with Euclidean local structure-based constraint

    Institute of Scientific and Technical Information of China (English)

    WANG Hongyuan; ZHANG Ji; CHEN Fuhua

    2016-01-01

    Sparse coding ( SC) based visual tracking ( l1⁃tracker) is gaining increasing attention, and many related algorithms are developed. In these algorithms, each candidate region is sparsely represented as a set of target tem⁃plates. However, the structure connecting these candidate regions is usually ignored. Lu proposed an NLSSC⁃tracker with non⁃local self⁃similarity sparse coding to address this issue, which has a high computational cost. In this study, we propose an Euclidean local⁃structure constraint based sparse coding tracker with a smoothed Euclidean local structure. With this tracker, the optimization procedure is transformed to a small⁃scale l1⁃optimization problem, sig⁃nificantly reducing the computational cost. Extensive experimental results on visual tracking demonstrate the effectiveness and efficiency of the proposed algorithm.

  18. Two-dimensional maximum local variation based on image euclidean distance for face recognition.

    Science.gov (United States)

    Gao, Quanxue; Gao, Feifei; Zhang, Hailin; Hao, Xiu-Juan; Wang, Xiaogang

    2013-10-01

    Manifold learning concerns the local manifold structure of high dimensional data, and many related algorithms are developed to improve image classification performance. None of them, however, consider both the relationships among pixels in images and the geometrical properties of various images during learning the reduced space. In this paper, we propose a linear approach, called two-dimensional maximum local variation (2DMLV), for face recognition. In 2DMLV, we encode the relationships among pixels in images using the image Euclidean distance instead of conventional Euclidean distance in estimating the variation of values of images, and then incorporate the local variation, which characterizes the diversity of images and discriminating information, into the objective function of dimensionality reduction. Extensive experiments demonstrate the effectiveness of our approach.

  19. Recovering Euclidean structure from principal-axes paralleled conics: applications to camera calibration.

    Science.gov (United States)

    Zhao, Zijian; Weng, Ying

    2014-06-01

    We focus on recovering the 2D Euclidean structure further for camera calibration from the projections of N parallel similar conics in this paper. This work demonstrates that the conic dual to the absolute points (CDAP) is the general form of the conic dual to the circular points, so it encodes the 2D Euclidean structure. However, the geometric size of the conic should be known if we utilize the CDAP. Under some special conditions (concentric conics), we proposed the rank-1 and rank-2 constraints. Our work relaxes the problem conditions and gives a more general framework than before. Experiments with simulated and real data are carried out to show the validity of the proposed algorithm.

  20. Modelling non-Euclidean movement and landscape connectivity in highly structured ecological networks

    Science.gov (United States)

    Sutherland, Christopher; Fuller, Angela K.; Royle, J. Andrew

    2015-01-01

    Movement is influenced by landscape structure, configuration and geometry, but measuring distance as perceived by animals poses technical and logistical challenges. Instead, movement is typically measured using Euclidean distance, irrespective of location or landscape structure, or is based on arbitrary cost surfaces. A recently proposed extension of spatial capture-recapture (SCR) models resolves this issue using spatial encounter histories of individuals to calculate least-cost paths (ecological distance: Ecology, 94, 2013, 287) thereby relaxing the Euclidean assumption. We evaluate the consequences of not accounting for movement heterogeneity when estimating abundance in highly structured landscapes, and demonstrate the value of this approach for estimating biologically realistic space-use patterns and landscape connectivity.

  1. Space, points and mereology. On foundations of point-free Euclidean geometry

    OpenAIRE

    Gruszczyński, Rafał; Pietruszczak, Andrzej

    2009-01-01

    This article is devoted to the problem of ontological foundations of three-dimensional Euclidean geometry. Starting from Bertrand Russell’s intuitions concerning the sensual world we try to show that it is possible to build a foundation for pure geometry by means of the so called regions of space. It is not our intention to present mathematically developed theory, but rather demonstrate basic assumptions, tools and techniques that are used in construction of systems of point-free geometry and...

  2. A modified mean curvature flow in Euclidean space and soap bubbles in symmetric spaces

    OpenAIRE

    Koike, Naoyuki

    2015-01-01

    In this paper, we show that small spherical soap bubbles in irreducible simply connected symmetric spaces of rank greater than one are constructed from the limits of a certain kind of modified mean curvature flows starting from small spheres in the Euclidean space of dimension equal to the rank of the symmetric space, where we note that the small spherical soap bubbles are invariant under the isotropy subgroup action of the isometry group of the symmetric space. Furthermore, we investigate th...

  3. Path Integral Discussion for Smorodinsky-Winternitz Potentials: I.\\ Two- and Three Dimensional Euclidean Space

    OpenAIRE

    Grosche, C.; Pogosyan, G. S.; Sissakian, A. N.

    1994-01-01

    Path integral formulations for the Smorodinsky-Winternitz potentials in two- and three-dimen\\-sional Euclidean space are presented. We mention all coordinate systems which separate the Smorodinsky-Winternitz potentials and state the corresponding path integral formulations. Whereas in many coordinate systems an explicit path integral formulation is not possible, we list in all soluble cases the path integral evaluations explicitly in terms of the propagators and the spectral expansions into t...

  4. Divide and Sprawl, Decline and Fall: A Comparative Critique of Euclidean Zoning

    Directory of Open Access Journals (Sweden)

    Eliza Hall

    2007-04-01

    Full Text Available As many commentators have pointed out, the land use patterns prevalent in the United States since the advent of Euclidean-style zoning have played a direct role in the development of a surprisingly broad range of problems: “[b]y fostering or requiring low density development with a high separation of uses, Euclidean zoning is one of the great generators of suburban sprawl, with all of its environmental, economic, and social costs.” These costs include pollution, loss of wilderness and farmland, racial and socioeconomic segregation of the population, and legal obstacles to effective urban rehabilitation.6 Moreover, in combination with prevailing patterns of local funding, the socioeconomic segregation caused by Euclidean zoning perpetuates itself by channeling less well-off children into chronically underequipped public schools and stretching the resources of many urban municipalities too thin, leaving them to choose between raising property tax rates or allowing their infrastructure to decay. That devil’s bargain bolsters the tendency of middle- and higher-income people to live in suburbs rather than cities, deepening the downward spiral in which many American cities find themselves. And the damage goes even further: “many current zoning practices disregard or even work against crime prevention goals” in both cities and suburbs. This is particularly problematic in light of the fact that “Euclidean systems of separation—conventional zoning—have been implemented ubiquitously” in the United States: “[a]bout ninety-seven percent of incorporated communities zone.”

  5. Parameterized runtime analyses of evolutionary algorithms for the planar euclidean traveling salesperson problem.

    Science.gov (United States)

    Sutton, Andrew M; Neumann, Frank; Nallaperuma, Samadhi

    2014-01-01

    Parameterized runtime analysis seeks to understand the influence of problem structure on algorithmic runtime. In this paper, we contribute to the theoretical understanding of evolutionary algorithms and carry out a parameterized analysis of evolutionary algorithms for the Euclidean traveling salesperson problem (Euclidean TSP). We investigate the structural properties in TSP instances that influence the optimization process of evolutionary algorithms and use this information to bound their runtime. We analyze the runtime in dependence of the number of inner points k. In the first part of the paper, we study a [Formula: see text] EA in a strictly black box setting and show that it can solve the Euclidean TSP in expected time [Formula: see text] where A is a function of the minimum angle [Formula: see text] between any three points. Based on insights provided by the analysis, we improve this upper bound by introducing a mixed mutation strategy that incorporates both 2-opt moves and permutation jumps. This strategy improves the upper bound to [Formula: see text]. In the second part of the paper, we use the information gained in the analysis to incorporate domain knowledge to design two fixed-parameter tractable (FPT) evolutionary algorithms for the planar Euclidean TSP. We first develop a [Formula: see text] EA based on an analysis by M. Theile, 2009, "Exact solutions to the traveling salesperson problem by a population-based evolutionary algorithm," Lecture notes in computer science, Vol. 5482 (pp. 145-155), that solves the TSP with k inner points in [Formula: see text] generations with probability [Formula: see text]. We then design a [Formula: see text] EA that incorporates a dynamic programming step into the fitness evaluation. We prove that a variant of this evolutionary algorithm using 2-opt mutation solves the problem after [Formula: see text] steps in expectation with a cost of [Formula: see text] for each fitness evaluation.

  6. Development of New Heuristics for the Euclidean Traveling SalesmanProblem

    OpenAIRE

    Tunnell, Thurman W.; Lenwood S. Heath

    1989-01-01

    Many heuristics have been developed to approximate optimal tours for the Euclidean Traveling Salesman Problem (ETSP). While much progress has been made, there are few quick heuristics which consistently produce tours within 4 percent of the optimal solution. This project examines a few of the well known heuristics and introduces two improvements, Maxdiff and Checks. Most algorithms, during tour constrution, add a city to the subtour because the city best satisfies some criterion. Maxdiff,...

  7. Solutions of the generalized Weierstrass representation in four-dimensional Euclidean space

    OpenAIRE

    P. Bracken; Grundland, A. M.

    2003-01-01

    Several classes of solutions of the generalized Weierstrass system, which induces constant mean curvature surfaces into four-dimensional Euclidean space are constructed. A gauge transformation allows us to simplify the system considered and derive factorized classes of solutions. A reduction of the generalized Weierstrass system to decoupled CP^1 sigma models is also considered. A new procedure for constructing certain classes of solutions, including elementary solutions (kinks and bumps) and...

  8. The generalized Weierstrass system inducing surfaces of constant and nonconstant mean curvature in Euclidean three space

    Science.gov (United States)

    Bracken, Paul

    2007-05-01

    The generalized Weierstrass (GW) system is introduced and its correspondence with the associated two-dimensional nonlinear sigma model is reviewed. The method of symmetry reduction is systematically applied to derive several classes of invariant solutions for the GW system. The solutions can be used to induce constant mean curvature surfaces in Euclidean three space. Some properties of the system for the case of nonconstant mean curvature are introduced as well.

  9. A generalized Hamiltonian Constraint Operator in Loop Quantum Gravity and its simplest Euclidean Matrix Elements

    OpenAIRE

    Gaul, Marcus; Rovelli, Carlo

    2000-01-01

    We study a generalized version of the Hamiltonian constraint operator in nonperturbative loop quantum gravity. The generalization is based on admitting arbitrary irreducible SU(2) representations in the regularization of the operator, in contrast to the original definition where only the fundamental representation is taken. This leads to a quantization ambiguity and to a family of operators with the same classical limit. We calculate the action of the Euclidean part of the generalized Hamilto...

  10. Derivatives, forms and vector fields on the kappa-deformed Euclidean space

    OpenAIRE

    Dimitrijevic, Marija; Moeller, Lutz; Tsouchnika, Efrossini

    2004-01-01

    The model of kappa-deformed space is an interesting example of a noncommutative space, since it allows a deformed symmetry. In this paper we present new results concerning different sets of derivatives on the coordinate algebra of kappa-deformed Euclidean space. We introduce a differential calculus with two interesting sets of one-forms and higher-order forms. The transformation law of vector fields is constructed in accordance with the transformation behaviour of derivatives. The crucial pro...

  11. Point Pattern Matching Algorithm for Planar Point Sets under Euclidean Transform

    Directory of Open Access Journals (Sweden)

    Xiaoyun Wang

    2012-01-01

    Full Text Available Point pattern matching is an important topic of computer vision and pattern recognition. In this paper, we propose a point pattern matching algorithm for two planar point sets under Euclidean transform. We view a point set as a complete graph, establish the relation between the point set and the complete graph, and solve the point pattern matching problem by finding congruent complete graphs. Experiments are conducted to show the effectiveness and robustness of the proposed algorithm.

  12. A functional technique based on the Euclidean algorithm with applications to 2-D acoustic diffractal diffusers

    Science.gov (United States)

    Cortés-Vega, Luis

    2015-09-01

    We built, based on the Euclidean algorithm, a functional technique, which allows to discover a direct proof of Chinese Remainder Theorem. Afterwards, by using this functional approach, we present some applications to 2-D acoustic diffractal diffusers. The novelty of the method is their functional algorithmic character, which improves ideas, as well as, other results of the author and his collaborators in a previous work.

  13. Euclidean Wilson loops and Minimal Area Surfaces in Minkowski AdS3

    CERN Document Server

    Irrgang, Andrew

    2015-01-01

    The AdS/CFT correspondence relates Wilson loops in N=4 SYM theory to minimal area surfaces in AdS5xS5 space. If the Wilson loop is Euclidean and confined to a plane (t,x) then the dual surface is Euclidean and lives in Minkowski AdS3. In this paper we study such minimal area surfaces generalizing previous results obtained in the Euclidean case. Since the surfaces we consider have the topology of a disk, the holonomy of the flat current vanishes which is equivalent to the condition that a certain boundary Schroedinger equation has all its solutions anti-periodic. If the potential for that Schroedinger equation is found then reconstructing the surface and finding the area become simpler. In particular we write a formula for the Area in terms of the Schwarzian derivative of the contour. Finally an infinite parameter family of analytical solutions using Riemann Theta functions is described. In this case, both the area and the shape of the surface are given analytically and used to check the previous results.

  14. Euclidean Wilson loops and minimal area surfaces in lorentzian AdS 3

    Science.gov (United States)

    Irrgang, Andrew; Kruczenski, Martin

    2015-12-01

    The AdS/CFT correspondence relates Wilson loops in N=4 SYM theory to minimal area surfaces in AdS 5 × S 5 space. If the Wilson loop is Euclidean and confined to a plane ( t, x) then the dual surface is Euclidean and lives in Lorentzian AdS 3 ⊂ AdS 5. In this paper we study such minimal area surfaces generalizing previous results obtained in the Euclidean case. Since the surfaces we consider have the topology of a disk, the holonomy of the flat current vanishes which is equivalent to the condition that a certain boundary Schrödinger equation has all its solutions anti-periodic. If the potential for that Schrödinger equation is found then reconstructing the surface and finding the area become simpler. In particular we write a formula for the Area in terms of the Schwarzian derivative of the contour. Finally an infinite parameter family of analytical solutions using Riemann Theta functions is described. In this case, both the area and the shape of the surface are given analytically and used to check the previous results.

  15. Fast Exact Euclidean Distance (FEED): A New Class of Adaptable Distance Transforms.

    Science.gov (United States)

    Schouten, Theo E; van den Broek, Egon L

    2014-11-01

    A new unique class of foldable distance transforms of digital images (DT) is introduced, baptized: Fast exact euclidean distance (FEED) transforms. FEED class algorithms calculate the DT starting-directly from the definition or rather its inverse. The principle of FEED class algorithms is introduced, followed by strategies for their efficient implementation. It is shown that FEED class algorithms unite properties of ordered propagation, raster scanning, and independent scanning DT. Moreover, FEED class algorithms shown to have a unique property: they can be tailored to the images under investigation. Benchmarks are conducted on both the Fabbri et al. data set and on a newly developed data set. Three baseline, three approximate, and three state-of-the-art DT algorithms were included, in addition to two implementations of FEED class algorithms. It illustrates that FEED class algorithms i) provide truly exact Euclidean DT; ii) do no suffer from disconnected Voronoi tiles, which is a unique feature for non-parallel but fast DT; iii) outperform any other approximate and exact Euclidean DT with its time complexity O(N), even after their optimization; and iv) are unequaled in that they can be adapted to the characteristics of the image class at hand.

  16. ETEA: a Euclidean minimum spanning tree-based evolutionary algorithm for multi-objective optimization.

    Science.gov (United States)

    Li, Miqing; Yang, Shengxiang; Zheng, Jinhua; Liu, Xiaohui

    2014-01-01

    The Euclidean minimum spanning tree (EMST), widely used in a variety of domains, is a minimum spanning tree of a set of points in space where the edge weight between each pair of points is their Euclidean distance. Since the generation of an EMST is entirely determined by the Euclidean distance between solutions (points), the properties of EMSTs have a close relation with the distribution and position information of solutions. This paper explores the properties of EMSTs and proposes an EMST-based evolutionary algorithm (ETEA) to solve multi-objective optimization problems (MOPs). Unlike most EMO algorithms that focus on the Pareto dominance relation, the proposed algorithm mainly considers distance-based measures to evaluate and compare individuals during the evolutionary search. Specifically, in ETEA, four strategies are introduced: (1) An EMST-based crowding distance (ETCD) is presented to estimate the density of individuals in the population; (2) A distance comparison approach incorporating ETCD is used to assign the fitness value for individuals; (3) A fitness adjustment technique is designed to avoid the partial overcrowding in environmental selection; (4) Three diversity indicators-the minimum edge, degree, and ETCD-with regard to EMSTs are applied to determine the survival of individuals in archive truncation. From a series of extensive experiments on 32 test instances with different characteristics, ETEA is found to be competitive against five state-of-the-art algorithms and its predecessor in providing a good balance among convergence, uniformity, and spread.

  17. Changes in serum cellular adhesion molecule and matrix metalloproteinase-9 levels in patients with cerebral infarction following hyperbaric oxygen therapy A case and intergroup control study

    Institute of Scientific and Technical Information of China (English)

    Renliang Zhao; Chunxia Wang; Yongjun Wang

    2008-01-01

    BACKGROUND: Animal studies have confirmed that hyperbaric oxygen (HBO) therapy can reduce matrix metalloproteinase activity and blood brain barrier permeability, thereby exhibiting neuroprotective effects. However, at present, consensus does not exist in terms of its clinical efficacy. OBJECTIVE: To validate the significance of changes in serum cellular adhesion molecule and MMP-9 levels in patients with cerebral infarction following HBO therapy. DESIGN, TIME AND SETTING: This randomized, controlled, neurobiochemical study was performed at the Department of Neurology, Affiliated Hospital of Qingdao University Medical College between December 2002 and March 2006. PARTICIPANTS: A total of 112 patients with acute cerebral infarction of internal carotid artery, comprising 64 males and 48 females, averaging (67 ± 11) years, were recruited and randomized to a HBO group (n = 50) and a routine treatment group (n = 62). An additional 30 gender- and age-matched normal subjects, consisting of 17 males and 13 females, averaging (63 ± 9) years, were enrolled as control subjects. METHODS: The routine treatment group received routine drug treatment and rehabilitation exercise. HBO treatment was additionally performed in the HBO group, once a day, for a total of 10 days. MAIN OUTCOME MEASURES: Serum levels of soluble intercellular adhesion molecule, soluble vascular cell adhesion molecule, soluble E-selectin, and matrix metalloproteinase-9 were detected by enzyme linked immunosorbent assay. RESULTS: Upon admission, serum levels of soluble intercellular adhesion molecule, soluble vascular cell adhesion molecule, soluble E-selectin, and matrix metalloproteinase-9 were significantly increased in patients with cerebral infarction, compared with control subjects (P < 0.01). Following HBO and routine treatments, serum levels of the above-mentioned indices were significantly reduced in the HBO and routine treatment groups (P < 0.01). Moreover, greater efficacy was observed in the HBO

  18. Hyperbolic cosines and sines theorems for the triangle formed by intersection of three semicircles on Euclidean plane

    CERN Document Server

    Yamaleev, Robert M

    2011-01-01

    The purpose the present paper is to construct the hyperbolic trigonometry on Euclidean plane without refereing to hyperbolic plane. In this paper we show that the concept of hyperbolic angle and its functions forming the hyperbolic trigonometry give arise on Euclidean plane in a natural way. The method is based on a key- formula establishing a relationship between exponential function and the ratio of two segments. This formula opens a straightforward pathway to hyperbolic trigonometry on the Euclidean plane. The hyperbolic law of cosines I and II and the hyperbolic law of sines are derived by using of the key-formula and the methods of Euclidean Geometry, only. It is shown that these laws are consequences of the interrelations between distances and radii of the intersecting semi-circles.

  19. Elastic line deformed on a pseudo-hypersurface by an external field in pseudo-Euclidean spaces

    Institute of Scientific and Technical Information of China (English)

    YCESAN; Ahmet; KEN; A.Ceylan

    2008-01-01

    We derive intrinsic formulation for elastic line deformed on a pseudo-hypersurface by an external field in the pseudo-Euclidean spaces E_v~n.This formulation determines elastic line deformed on a pseudo-hypersurface.

  20. System-level insights into the cellular interactome of a non-model organism: inferring, modelling and analysing functional gene network of soybean (Glycine max.

    Directory of Open Access Journals (Sweden)

    Yungang Xu

    Full Text Available Cellular interactome, in which genes and/or their products interact on several levels, forming transcriptional regulatory-, protein interaction-, metabolic-, signal transduction networks, etc., has attracted decades of research focuses. However, such a specific type of network alone can hardly explain the various interactive activities among genes. These networks characterize different interaction relationships, implying their unique intrinsic properties and defects, and covering different slices of biological information. Functional gene network (FGN, a consolidated interaction network that models fuzzy and more generalized notion of gene-gene relations, have been proposed to combine heterogeneous networks with the goal of identifying functional modules supported by multiple interaction types. There are yet no successful precedents of FGNs on sparsely studied non-model organisms, such as soybean (Glycine max, due to the absence of sufficient heterogeneous interaction data. We present an alternative solution for inferring the FGNs of soybean (SoyFGNs, in a pioneering study on the soybean interactome, which is also applicable to other organisms. SoyFGNs exhibit the typical characteristics of biological networks: scale-free, small-world architecture and modularization. Verified by co-expression and KEGG pathways, SoyFGNs are more extensive and accurate than an orthology network derived from Arabidopsis. As a case study, network-guided disease-resistance gene discovery indicates that SoyFGNs can provide system-level studies on gene functions and interactions. This work suggests that inferring and modelling the interactome of a non-model plant are feasible. It will speed up the discovery and definition of the functions and interactions of other genes that control important functions, such as nitrogen fixation and protein or lipid synthesis. The efforts of the study are the basis of our further comprehensive studies on the soybean functional

  1. The co-adaptive neural network approach to the Euclidean Travelling Salesman Problem.

    Science.gov (United States)

    Cochrane, E M; Beasley, J E

    2003-12-01

    In this paper we consider the Euclidean Travelling Salesman Problem (ETSP). This is the problem of finding the shortest tour around a number of cities where the cities correspond to points in the Euclidean plane and the distances between cities are given by the usual Euclidean distance metric. We present a review of the literature with respect to neural network (NN) approaches for the ETSP, and the computational results that have been reported. Based upon this review we highlight two areas that are, in our judgement, currently neglected/lacking in the literature. These are: failure to make significant use of publicly available ETSP test problems in computational work, failure to address co-operation between neurons. Drawing upon our literature survey this paper presents a new Self-Organising NN approach, called the Co-Adaptive Net, which involves not just unsupervised learning to train neurons, but also allows neurons to co-operate and compete amongst themselves depending on their situation. Our Co-Adaptive Net algorithm also includes a number of algorithmic mechanisms that, based upon our literature review, we consider to have contributed to the computational success of previous algorithms. Results for 91 publicly available standard ETSP's are presented in this paper. The largest of these problems involves 85,900 cities. This paper presents: the most extensive computational evaluation of any NN approach on publicly available ETSP test problems that has been made to date in the literature, a NN approach that performs better, with respect to solution quality and/or computation time, than other NN approaches given previously in the literature. Drawing upon computational results produced as a result of the DIMACS TSP Challenge, we highlight the fact that none of the current NN approaches for the ETSP can compete with state of the art Operations Research heuristics. We discuss why we consider continuing to study and develop NN approaches for the ETSP to be of value.

  2. Non-perturbative BRST quantization of Euclidean Yang-Mills theories in Curci-Ferrari gauges

    CERN Document Server

    Pereira, A D; Sorella, S P

    2016-01-01

    In this paper we address the issue of the non-perturbative quantization of Euclidean Yang-Mills theories in the Curci-Ferrari gauge. In particular, we construct a Refined Gribov-Zwanziger action for this gauge which takes into account the presence of gauge copies as well as the dynamical formation of dimension two condensates. This action enjoys a non-perturbative BRST symmetry recently proposed in \\cite{Capri:2015ixa}. Finally, we give attention to the gluon propagator in different space-time dimensions.

  3. Pseudo Euclidean-Signature Harmonic Oscillator, Quantum Field Theory and Vanishing Cosmological Constant

    OpenAIRE

    Pavsic, Matej

    1998-01-01

    The harmonic oscillator in pseudo euclidean space is studied. A straightforward procedure reveals that although such a system may have negative energy, it is stable. In the quantized theory the vacuum state has to be suitably defined and then the zero-point energy corresponding to a positive-signature component is canceled by the one corresponding to a negative-signature component. This principle is then applied to a system of scalar fields. The metric in the space of fields is assumed to hav...

  4. Recognition of Facial Expression Using Eigenvector Based Distributed Features and Euclidean Distance Based Decision Making Technique

    Directory of Open Access Journals (Sweden)

    Jeemoni Kalita

    2013-03-01

    Full Text Available In this paper, an Eigenvector based system has been presented to recognize facial expressions from digital facial images. In the approach, firstly the images were acquired and cropping of five significant portions from the image was performed to extract and store the Eigenvectors specific to the expressions. The Eigenvectors for the test images were also computed, and finally the input facial image was recognized when similarity was obtained by calculating the minimum Euclidean distance between the test image and the different expressions.

  5. Emergence of Euclidean dynamical symmetry as a consequence of shape phase mixing

    OpenAIRE

    Budaca, R.; Budaca, A. I.

    2016-01-01

    A hybrid model which combines γ-stable and γ-rigid collective conditions through a rigidity parameter, is used to study the critical point of the phase transition between spherical and axially symmetric shapes. The model in the equally mixed case, called X(4), exhibits properties of the Euclidean symmetry in four dimensions. The spectral properties of the new model are investigated in connection to the exact symmetry. Experimental realisation of the X(4) model is found in two N=90 nuclei and ...

  6. Stability transitions for axisymmetric relative equilibria of Euclidean symmetric Hamiltonian systems

    CERN Document Server

    Patrick, G W; Wulff, C

    2007-01-01

    In the presence of noncompact symmetry, the stability of relative equilibria under momentum preserving perturbations does not generally imply robust stability under momentum changing perturbations. For axisymmetric relative equilibria of Hamiltonian systems with Euclidean symmetry, we investigate different mechanisms of stability: stability by energy-momentum confinement, KAM, and Nekhoroshev stability, and we explain the transitions between these. We apply our results to the Kirchhoff model for the motion of an axisymmetric underwater vehicle, and we numerically study dissipation induced instability of KAM stable relative equilibria for this system.

  7. A SKELETONIZATION ALGORITHM BASED ON EUCLIDEAN DISTANCE MAPS AND MORPHOLOGICAL OPERATORS

    Institute of Scientific and Technical Information of China (English)

    Ma Lihong; Yu Yinglin; Zhang Yu

    2001-01-01

    In this letter a new skeletonization algorithm is proposed. It combines techniques of fast construction of Euclidean Distance Maps(EDMs), ridge extraction, Hit-or-Miss Transformation(HMT) of structuring elements and the set operators. It first produces the EDM image with no more than 4 passes through an image of any kinds, and then the ridge image is extracted by applying a turn-on scheme and performing a rain-fall elimination to accelerate the processing. The one-pixel wide skeleton is finally acquired by carrying out the HMTs of two structure elements and the SUBTRACT and OR operations. Experimental results obtained by practical applications are also presented.

  8. Extending breath analysis to the cellular level: current thoughts on the human microbiome and the expression of organic compounds in the human exposome

    Science.gov (United States)

    Human biomarkers are comprised of compounds from cellular metabolism, oxidative stress, and the microbiome of bacteria in the gut, genitourinary, and pulmonary tracts. When we examine patterns in human biomarkers to discern human health state or diagnose specific diseases, it is...

  9. Single and multiple object tracking using log-euclidean Riemannian subspace and block-division appearance model.

    Science.gov (United States)

    Hu, Weiming; Li, Xi; Luo, Wenhan; Zhang, Xiaoqin; Maybank, Stephen; Zhang, Zhongfei

    2012-12-01

    Object appearance modeling is crucial for tracking objects, especially in videos captured by nonstationary cameras and for reasoning about occlusions between multiple moving objects. Based on the log-euclidean Riemannian metric on symmetric positive definite matrices, we propose an incremental log-euclidean Riemannian subspace learning algorithm in which covariance matrices of image features are mapped into a vector space with the log-euclidean Riemannian metric. Based on the subspace learning algorithm, we develop a log-euclidean block-division appearance model which captures both the global and local spatial layout information about object appearances. Single object tracking and multi-object tracking with occlusion reasoning are then achieved by particle filtering-based Bayesian state inference. During tracking, incremental updating of the log-euclidean block-division appearance model captures changes in object appearance. For multi-object tracking, the appearance models of the objects can be updated even in the presence of occlusions. Experimental results demonstrate that the proposed tracking algorithm obtains more accurate results than six state-of-the-art tracking algorithms. PMID:22331855

  10. Single and multiple object tracking using log-euclidean Riemannian subspace and block-division appearance model.

    Science.gov (United States)

    Hu, Weiming; Li, Xi; Luo, Wenhan; Zhang, Xiaoqin; Maybank, Stephen; Zhang, Zhongfei

    2012-12-01

    Object appearance modeling is crucial for tracking objects, especially in videos captured by nonstationary cameras and for reasoning about occlusions between multiple moving objects. Based on the log-euclidean Riemannian metric on symmetric positive definite matrices, we propose an incremental log-euclidean Riemannian subspace learning algorithm in which covariance matrices of image features are mapped into a vector space with the log-euclidean Riemannian metric. Based on the subspace learning algorithm, we develop a log-euclidean block-division appearance model which captures both the global and local spatial layout information about object appearances. Single object tracking and multi-object tracking with occlusion reasoning are then achieved by particle filtering-based Bayesian state inference. During tracking, incremental updating of the log-euclidean block-division appearance model captures changes in object appearance. For multi-object tracking, the appearance models of the objects can be updated even in the presence of occlusions. Experimental results demonstrate that the proposed tracking algorithm obtains more accurate results than six state-of-the-art tracking algorithms.

  11. Equivalence between Euclidean and in-in formalisms in de Sitter QFT

    Science.gov (United States)

    Higuchi, Atsushi; Marolf, Donald; Morrison, Ian A.

    2011-04-01

    We study the relation between two sets of correlators in interacting quantum field theory on de Sitter space. The first are correlators computed using in-in perturbation theory in the expanding cosmological patch of de Sitter space (also known as the conformal patch, or the Poincaré patch), and for which the free propagators are taken to be those of the free Euclidean vacuum. The second are correlators obtained by analytic continuation from Euclidean de Sitter; i.e., they are correlators in the fully interacting Hartle-Hawking state. We give an analytic argument that these correlators coincide for interacting massive scalar fields with any m2>0. We also verify this result via direct calculation in simple examples. The correspondence holds diagram by diagram, and at any finite value of an appropriate Pauli-Villars regulator mass M. Along the way, we note interesting connections between various prescriptions for perturbation theory in general static spacetimes with bifurcate Killing horizons.

  12. Bi-Lipshitz Embedding of Ultrametric Cantor Sets into Euclidean Spaces

    CERN Document Server

    Bellissard, Jean V

    2012-01-01

    Let $(C,d)$ be an ultrametric Cantor set. Then it admits an isometric embedding into an infinite dimensional Euclidean space \\cite{PB08}. Associated with it is a weighted rooted tree, the reduced Michon graph $\\mathscr T$ \\cite{Mi85}. It will be called $f$-embeddable if there is a bi-Lipshitz map from $(C,d)$ into a finite dimensional Euclidean space. The main result establishes that $(C,d)$ is $f$-embeddable if and only if it can be represented by a weighted Michon tree such that (i) the number of children per vertex is uniformly bounded, (ii) if $\\kappa$ denotes the weight, there are constants $c>0$ and $0<\\delta<1$ such that $\\kappa(v)/\\kappa(u)\\leq c\\,\\delta^{d(u,v)}$ where $v$ is a descendant of $u$ and where $d(u,v)$ denotes the graph distance between the vertices $u,v$. Several examples are provided: (a) the tiling space of a linear repetitive sequence is $f$-embeddable, (b) the tiling space of a Sturmian sequence is $f$-embeddable if and only if the irrational number characterizing it has bounde...

  13. Facial expression recognition based on image Euclidean distance-supervised neighborhood preserving embedding

    Science.gov (United States)

    Chen, Li; Li, Yingjie; Li, Haibin

    2014-11-01

    High-dimensional data often lie on relatively low-dimensional manifold, while the nonlinear geometry of that manifold is often embedded in the similarities between the data points. These similar structures are captured by Neighborhood Preserving Embedding (NPE) effectively. But NPE as an unsupervised method can't utilize class information to guide the procedure of nonlinear dimensionality reduction. They ignore the geometrical structure information of local data points and the spatial information of pixels, which leads to the failure of classification. For this problem, a feature extraction method based on Image Euclidean Distance-Supervised NPE (IED-SNPE) is proposed, and is applied to facial expression recognition. Firstly, it employs Image Euclidean Distance (IED) to characterize the dissimilarity of data points. And then the neighborhood graph of the input data is constructed according to a certain kind of dissimilarity between data points. Finally, it fuses prior nonlinear facial expression manifold of facial expression images and class-label information to extract discriminative features for expression recognition. In the classification experiments on JAFFE facial expression database, IED-SNPE is used for feature extraction and compared with NPE, SNPE, and IED-NPE. The results reveal that IED-SNPE not only the local structure of expression manifold preserves well but also explicitly considers the spatial relationships among pixels in the images. So it excels NPE in feature extraction and is highly competitive with those well-known feature extraction methods.

  14. Implementations of a Parallel Algorithm for Computing Euclidean Distance Map in Multicore Processors and GPUs

    Directory of Open Access Journals (Sweden)

    Yasuaki Ito

    2011-07-01

    Full Text Available Given a 2-D binary image of size n×n, Euclidean Distance Map (EDM is a 2-D array of the same size such that each element is storing the Euclidean distance to the nearest black pixel. It is known that a sequential algorithm can compute the EDM in O(n2 and thus this algorithm is optimal. Also, work-time optimal parallel algorithms for shared memory model have been presented. However, the presented parallel algorithms are too complicated to implement in existing shared memory parallel machines. The main contribution of this paper is to develop a simple parallel algorithm for the EDM and implement it in two different parallel platforms: multicore processors and Graphics Processing Units (GPUs. We have implemented our parallel algorithm in a Linux server with four Intel hexad-core processors (Intel Xeon X7460 2.66GHz. We have also implemented it in the following two modern GPU systems, Tesla C1060 and GTX 480, respectively. The experimental results have shown that, for an input binary image with size of 9216×9216, our implementation in the multicore system achieves a speedup factor of 18 over the performance of a sequential algorithm using a single processor in the same system. Meanwhile, for the same input binary image, our implementation on the GPU achieves a speedup factor of 26 over the sequential algorithm implementation.

  15. Simple Motion Pursuit and Evasion Differential Games with Many Pursuers on Manifolds with Euclidean Metric

    Directory of Open Access Journals (Sweden)

    Atamurat Kuchkarov

    2016-01-01

    Full Text Available We consider pursuit and evasion differential games of a group of m pursuers and one evader on manifolds with Euclidean metric. The motions of all players are simple, and maximal speeds of all players are equal. If the state of a pursuer coincides with that of the evader at some time, we say that pursuit is completed. We establish that each of the differential games (pursuit or evasion is equivalent to a differential game of m groups of countably many pursuers and one group of countably many evaders in Euclidean space. All the players in any of these groups are controlled by one controlled parameter. We find a condition under which pursuit can be completed, and if this condition is not satisfied, then evasion is possible. We construct strategies for the pursuers in pursuit game which ensure completion the game for a finite time and give a formula for this time. In the case of evasion game, we construct a strategy for the evader.

  16. A simplified procedure for correcting both errors and erasures of a Reed-Solomon code using the Euclidean algorithm

    Science.gov (United States)

    Truong, T. K.; Hsu, I. S.; Eastman, W. L.; Reed, I. S.

    1987-01-01

    It is well known that the Euclidean algorithm or its equivalent, continued fractions, can be used to find the error locator polynomial and the error evaluator polynomial in Berlekamp's key equation needed to decode a Reed-Solomon (RS) code. A simplified procedure is developed and proved to correct erasures as well as errors by replacing the initial condition of the Euclidean algorithm by the erasure locator polynomial and the Forney syndrome polynomial. By this means, the errata locator polynomial and the errata evaluator polynomial can be obtained, simultaneously and simply, by the Euclidean algorithm only. With this improved technique the complexity of time domain RS decoders for correcting both errors and erasures is reduced substantially from previous approaches. As a consequence, decoders for correcting both errors and erasures of RS codes can be made more modular, regular, simple, and naturally suitable for both VLSI and software implementation. An example illustrating this modified decoding procedure is given for a (15, 9) RS code.

  17. Changes of anabolic processes at the cellular and molecular level in chronic wounds under topical negative pressure can be revealed by transcriptome analysis

    OpenAIRE

    Leffler, Mareike; Derrick, Kathleen L.; McNulty, Amy; Malsiner, Caye; Dragu, Adrian; Horch, Raymund E.

    2011-01-01

    Abstract Chronic wounds – as defined by the World Union of Wound Healing Societies (WUWHS) – are a considerable worldwide health care expense and impair quality of life. In order for chronic wounds to heal, these wounds must be transformed to a more acute state to begin the healing process. Topical negative pressure (TNP) with reticulated open cell foam (ROCF) is known to promote healing in certain types of chronic wounds. However, little is known about changes at the cellular or molecular le...

  18. Phase retrieval by Euclidean distance in self-calibrating generalized phase-shifting interferometry of three steps.

    Science.gov (United States)

    Meneses-Fabian, Cruz; Lara-Cortes, Francisco Alejandro

    2015-05-18

    This paper presents a novel algorithm for phase extraction based on the computation of the Euclidean distance from a point to an ellipse. The idea consists in extracting the intensities from a data row or column in three interferograms to form points of intensity and then fitting them to an ellipse by the method of least squares. The Euclidean distance for each intensity point is computed to find a parametric phase whose value is associated to the object phase. The main advantage of the present method is to avoid the use of tangent function, reducing the error in the desired phase computation.

  19. Molecular and Cellular Signaling

    CERN Document Server

    Beckerman, Martin

    2005-01-01

    A small number of signaling pathways, no more than a dozen or so, form a control layer that is responsible for all signaling in and between cells of the human body. The signaling proteins belonging to the control layer determine what kinds of cells are made during development and how they function during adult life. Malfunctions in the proteins belonging to the control layer are responsible for a host of human diseases ranging from neurological disorders to cancers. Most drugs target components in the control layer, and difficulties in drug design are intimately related to the architecture of the control layer. Molecular and Cellular Signaling provides an introduction to molecular and cellular signaling in biological systems with an emphasis on the underlying physical principles. The text is aimed at upper-level undergraduates, graduate students and individuals in medicine and pharmacology interested in broadening their understanding of how cells regulate and coordinate their core activities and how diseases ...

  20. Distributed stochastic multi-vehicle routing in the Euclidean plane with no communications

    Science.gov (United States)

    Pietrabissa, Antonio

    2016-08-01

    This paper presents an algorithm for the multi-vehicle routing problem with no communications among the vehicles. The scenario consists in a convex Euclidean mission space, where targets are generated according to a Poisson distribution in time and to a generic continuous spatial distribution. The targets must be visited by the vehicles, which, therefore, must act in coordination. Even if no communications are required, the proposed routing strategy succeeds in effectively partitioning the mission space among the vehicles: at low target generation rates, the algorithm leads to the well-known centroidal Voronoi tessellation, whereas at high target generation rates, simulation results show that it has better performances with respect to a reference algorithm with no communications among vehicles.

  1. Clear evidence of a continuum theory of 4D Euclidean simplicial quantum gravity

    Science.gov (United States)

    Egawa, H. S.; Horata, S.; Yukawa, T.

    2002-03-01

    Four-dimensional (4D) simplicial quantum gravity coupled to both scalar fields ( NX) and gauge fields ( NA) has been studied using Monte-Carlo simulations. The matter dependence of the string susceptibility exponent γ (4) is estimated. Furthermore, we compare our numerical results with Background-Metric-Indepenent (BMI) formulation conjectured to describe the quantum field theory of gravity in 4D. The numerical results suggest that the 4D simplicial quantum gravity is related to the conformal gravity in 4D. Therefore, we propose a phase structure in detail with adding both scalar and gauge fields and discuss the possibility and the property of a continuum theory of 4D Euclidean simplicial quantum gravity.

  2. Clear Evidence of a Continuum Theory of 4D Euclidean Simplicial Quantum Gravity

    CERN Document Server

    Egawa, H S; Yukawa, T

    2002-01-01

    Four-dimensional (4D) simplicial quantum gravity coupled to both scalar fields (N_X) and gauge fields (N_A) has been studied using Monte-Carlo simulations. The matter dependence of the string susceptibility exponent gamma^{(4)} is estimated. Furthermore, we compare our numerical results with Background-Metric-Independent (BMI) formulation conjectured to describe the quantum field theory of gravity in 4D. The numerical results suggest that the 4D simplicial quantum gravity is related to the conformal gravity in 4D. Therefore, we propose a phase structure in detail with adding both scalar and gauge fields and discuss the possibility and the property of a continuum theory of 4D Euclidean simplicial quantum gravity.

  3. Existence and Blowup Results for Asymptotically Euclidean Initial Data Sets Generated by the Conformal Method

    CERN Document Server

    Dilts, James

    2016-01-01

    For each set of (freely chosen) seed data, the conformal method reduces the Einstein constraint equations to a system of elliptic equations, the conformal constraint equations. We prove an admissibility criterion, based on a (conformal) prescribed scalar curvature problem, which provides a necessary condition on the seed data for the conformal constraint equations to (possibly) admit a solution. We then consider sets of asymptotically Euclidean (AE) seed data for which solutions of the conformal constraint equations exist, and examine the blowup properties of these solutions as the seed data sets approach sets for which no solutions exist. We also prove that there are AE seed data sets which include a Yamabe nonpositive metric and lead to solutions of the conformal constraints. These data sets allow the mean curvature function to have zeroes.

  4. New results on embeddings of polyhedra and manifolds in Euclidean spaces

    International Nuclear Information System (INIS)

    The aim of this survey is to present several classical results on embeddings and isotopies of polyhedra and manifolds in Rm. We also describe the revival of interest in this beautiful branch of topology and give an account of new results, including an improvement of the Haefliger-Weber theorem on the completeness of the deleted product obstruction to embeddability and isotopy of highly connected manifolds in Rm (Skopenkov) as well as the unimprovability of this theorem for polyhedra (Freedman, Krushkal, Teichner, Segal, Skopenkov, and Spiez) and for manifolds without the necessary connectedness assumption (Skopenkov). We show how algebraic obstructions (in terms of cohomology, characteristic classes, and equivariant maps) arise from geometric problems of embeddability in Euclidean spaces. Several classical and modern results on completeness or incompleteness of these obstructions are stated and proved. By these proofs we illustrate classical and modern tools of geometric topology (engulfing, the Whitney trick, van Kampen and Casson finger moves, and their generalizations)

  5. Clifford algebra-valued orthogonal polynomials in the open unit ball of Euclidean space

    Directory of Open Access Journals (Sweden)

    Fred Brackx

    2004-01-01

    Full Text Available A new method for constructing Clifford algebra-valued orthogonal polynomials in the open unit ball of Euclidean space is presented. In earlier research, we only dealt with scalar-valued weight functions. Now the class of weight functions involved is enlarged to encompass Clifford algebra-valued functions. The method consists in transforming the orthogonality relation on the open unit ball into an orthogonality relation on the real axis by means of the so-called Clifford-Heaviside functions. Consequently, appropriate orthogonal polynomials on the real axis give rise to Clifford algebra-valued orthogonal polynomials in the unit ball. Three specific examples of such orthogonal polynomials in the unit ball are discussed, namely, the generalized Clifford-Jacobi polynomials, the generalized Clifford-Gegenbauer polynomials, and the shifted Clifford-Jacobi polynomials.

  6. Non-Abelian Ball-Chiu vertex for arbitrary Euclidean momenta

    CERN Document Server

    Aguilar, A C; Ferreira, M N; Papavassiliou, J

    2016-01-01

    We determine the non-Abelian version of the four longitudinal form factors of the quark-gluon vertex, using exact expressions derived from the Slavnov-Taylor identity that this vertex satisfies. In addition to the quark and ghost propagators, a key ingredient of the present approach is the quark-ghost scattering kernel, which is computed within the one-loop dressed approximation. The vertex form factors obtained from this procedure are evaluated for arbitrary Euclidean momenta, and display features not captured by the well-known Ball-Chiu vertex, deduced from the Abelian (ghost-free) Ward identity. The potential phenomenological impact of these results is evaluated through the study of special renormalization-point-independent combinations, which quantify the strength of the interaction kernels appearing in the standard quark gap and Bethe-Salpeter equations.

  7. Approximation Algorithm for Bottleneck Steiner Tree Problem in the Euclidean Plane

    Institute of Scientific and Technical Information of China (English)

    Zi-Mao Li; Da-Ming Zhu; Shao-Han Ma

    2004-01-01

    A special case of the bottleneck Steiner tree problem in the Euclidean plane was considered in this paper. The problem has applications in the design of wireless communication networks, multifacility location, VLSI routing and network routing. For the special case which requires that there should be no edge connecting any two Steiner points in the optimal solution, a 3-restricted Steiner tree can be found indicating the existence of the performance ratio √2. In this paper, the special case of the problem is proved to be NP-hard and cannot be approximated within ratio √2. First a simple polynomial time approximation algorithm with performance ratio √3 is presented. Then based on this algorithm and the existence of the 3-restricted Steiner tree, a polynomial time approximation algorithm with performance ratio-√2 + ε is proposed, for any ε>0.

  8. Derivatives, forms and vector fields on the κ-deformed Euclidean space

    International Nuclear Information System (INIS)

    The model of κ-deformed space is an interesting example of a noncommutative space, since it allows a deformed symmetry. In this paper, we present new results concerning different sets of derivatives on the coordinate algebra of κ-deformed Euclidean space. We introduce a differential calculus with two interesting sets of one-forms and higher-order forms. The transformation law of vector fields is constructed in accordance with the transformation behaviour of derivatives. The crucial property of the different derivatives, forms and vector fields is that in an n-dimensional spacetime there are always n of them. This is the key difference with respect to conventional approaches, in which the differential calculus is (n + 1)-dimensional. This work shows that derivative-valued quantities such as derivative-valued vector fields appear in a generic way on noncommutative spaces

  9. Three-frame generalized phase-shifting interferometry by a Euclidean matrix norm algorithm

    Science.gov (United States)

    Xu, Yuanyuan; Wang, Yawei; Ji, Ying; Han, Hao; Jin, Weifeng

    2016-09-01

    Generalized phase-shifting interferometry (GPSI) is one of the most effective techniques in imaging of a phase object, in which phase retrieval is an essential and important procedure. In this paper, a simple and rapid algorithm for retrieval of the unknown phase shifts in three-frame GPSI is proposed. Using this algorithm, the value of phase shift can be calculated by a determinate formula consisting of three different Euclidean matrix norms of the intensity difference between two phase shifted interferograms, and then the phase can be retrieved easily. The algorithm has the advantages of freeing from the background elimination and less computation, since it only needs three phase-shifted interferograms without no extra measurements, the iterative procedure or the integral transformation. The reliability and accuracy of this algorithm were demonstrated by simulation and experimental results.

  10. New efficient algorithm for the isometric embedding of 2-surface metrics in three dimensional Euclidean space

    Science.gov (United States)

    Tichy, Wolfgang; McDonald, Jonathan R.; Miller, Warner A.

    2015-01-01

    We present a new numerical method for the isometric embedding of 2-geometries specified by their 2-metrics in three-dimensional Euclidean space. Our approach is to directly solve the fundamental embedding equation supplemented by six conditions that fix translations and rotations of the embedded surface. This set of equations is discretized by means of a pseudospectral collocation point method. The resulting nonlinear system of equations are then solved by a Newton-Raphson scheme. We explain our numerical algorithm in detail. By studying several examples we show that our method converges provided we start the Newton-Raphson scheme from a suitable initial guess. Our novel method is very efficient for smooth 2-metrics.

  11. New efficient algorithm for the isometric embedding of 2-surface metrics in 3 dimensional Euclidean space

    CERN Document Server

    Tichy, Wolfgang; Miller, Warner A

    2014-01-01

    We present a new numerical method for the isometric embedding of 2-geometries specified by their 2-metrics in three dimensional Euclidean space. Our approach is to directly solve the fundamental embedding equation supplemented by six conditions that fix translations and rotations of the embedded surface. This set of equations is discretized by means of a pseudospectral collocation point method. The resulting nonlinear system of equations are then solved by a Newton-Raphson scheme. We explain our numerical algorithm in detail. By studying several examples we show that our method converges provided we start the Newton-Raphson scheme from a suitable initial guess. Our novel method is very efficient for smooth 2-metrics.

  12. Artificial immune system via Euclidean Distance Minimization for anomaly detection in bearings

    Science.gov (United States)

    Montechiesi, L.; Cocconcelli, M.; Rubini, R.

    2016-08-01

    In recent years new diagnostics methodologies have emerged, with particular interest into machinery operating in non-stationary conditions. In fact continuous speed changes and variable loads make non-trivial the spectrum analysis. A variable speed means a variable characteristic fault frequency related to the damage that is no more recognizable in the spectrum. To overcome this problem the scientific community proposed different approaches listed in two main categories: model-based approaches and expert systems. In this context the paper aims to present a simple expert system derived from the mechanisms of the immune system called Euclidean Distance Minimization, and its application in a real case of bearing faults recognition. The proposed method is a simplification of the original process, adapted by the class of Artificial Immune Systems, which proved to be useful and promising in different application fields. Comparative results are provided, with a complete explanation of the algorithm and its functioning aspects.

  13. Unstable spiral waves and local Euclidean symmetry in a model of cardiac tissue

    Energy Technology Data Exchange (ETDEWEB)

    Marcotte, Christopher D.; Grigoriev, Roman O. [School of Physics, Georgia Institute of Technology, Atlanta, Georgia 30332 (United States)

    2015-06-15

    This paper investigates the properties of unstable single-spiral wave solutions arising in the Karma model of two-dimensional cardiac tissue. In particular, we discuss how such solutions can be computed numerically on domains of arbitrary shape and study how their stability, rotational frequency, and spatial drift depend on the size of the domain as well as the position of the spiral core with respect to the boundaries. We also discuss how the breaking of local Euclidean symmetry due to finite size effects as well as the spatial discretization of the model is reflected in the structure and dynamics of spiral waves. This analysis allows identification of a self-sustaining process responsible for maintaining the state of spiral chaos featuring multiple interacting spirals.

  14. Patching up the No-Boundary Proposal with virtual Euclidean wormholes

    CERN Document Server

    Bousso, R; Bousso, Raphael; Chamblin, Andrew

    1999-01-01

    In quantum cosmology, one often considers tunneling phenomena which may have occurred in the early universe. Processes requiring quantum penetration of a potential barrier include black hole pair creation and the decay of vacuum domain walls. Ideally, one calculates the rates for such processes by finding an instanton, or Euclidean solution of the field equations, which interpolates between the initial and final states. In practice, however, it has become customary to calculate such amplitudes using the No-Boundary Proposal of Hartle and Hawking. A criticism of this method is that it does not use a single path which interpolates between the initial and final states, but two disjoint instantons: One divides the probability to create the final state from nothing by the probability to create the initial state from nothing and decrees the answer to be the rate of tunneling from the initial to the final state. Here, we demonstrate the validity of this approach by constructing continuous paths connecting the ingoin...

  15. Development and implementation of cellular-based real-time reporting and internet accessible coastal sea-level gauge - A vital tool for monitoring storm surge and tsunami

    Digital Repository Service at National Institute of Oceanography (India)

    Desai, R.G.P.; Joseph, A.; Agarvadekar, Y.; Dabholkar, N.; Mehra, P.; Gouveia, A.D.; Tengali, S.; VijayKumar, K.; Parab, A.

    illustration of the predicted fair-weather sea-level, current sea-level, and residual sea-level (i.e. measured minus predicted fairweather sea-level), which can be monitored via Internet from any part of the world. This system provides a cost...

  16. Reducing residual stresses and deformations in selective laser melting through multi-level multi-scale optimization of cellular scanning strategy

    Science.gov (United States)

    Mohanty, Sankhya; Hattel, Jesper H.

    2016-04-01

    Residual stresses and deformations continue to remain one of the primary challenges towards expanding the scope of selective laser melting as an industrial scale manufacturing process. While process monitoring and feedback-based process control of the process has shown significant potential, there is still dearth of techniques to tackle the issue. Numerical modelling of selective laser melting process has thus been an active area of research in the last few years. However, large computational resource requirements have slowed the usage of these models for optimizing the process. In this paper, a calibrated, fast, multiscale thermal model coupled with a 3D finite element mechanical model is used to simulate residual stress formation and deformations during selective laser melting. The resulting reduction in thermal model computation time allows evolutionary algorithm-based optimization of the process. A multilevel optimization strategy is adopted using a customized genetic algorithm developed for optimizing cellular scanning strategy for selective laser melting, with an objective of reducing residual stresses and deformations. The resulting thermo-mechanically optimized cellular scanning strategies are compared with standard scanning strategies and have been used to manufacture standard samples.

  17. Euclidean Skeletons of Digital Image and Volume Data in Linear Time by the Integer Medial Axis Transform

    NARCIS (Netherlands)

    Hesselink, Wim H.; Roerdink, Jos B.T.M.

    2008-01-01

    A general algorithm for computing euclidean skeletons of 2D and 3D data sets in linear time is presented. These skeletons are defined in terms of a new concept, called the integer medial axis (IMA) transform. We prove a number of fundamental properties of the IMA skeleton and compare these with prop

  18. Cellular Barcoding Links B-1a B Cell Potential to a Fetal Hematopoietic Stem Cell State at the Single-Cell Level

    DEFF Research Database (Denmark)

    Kristiansen, Trine A; Jaensson Gyllenbäck, Elin; Zriwil, Alya;

    2016-01-01

    Hematopoietic stem cells (HSCs) undergo a functional switch in neonatal mice hallmarked by a decrease in self-renewing divisions and entry into quiescence. Here, we investigated whether the developmental attenuation of B-1a cell output is a consequence of a shift in stem cell state during ontogeny....... Using cellular barcoding for in vivo single-cell fate analyses, we found that fetal liver definitive HSCs gave rise to both B-1a and B-2 cells. Whereas B-1a potential diminished in all HSCs with time, B-2 output was maintained. B-1a and B-2 plasticity could be reinitiated in a subset of adult HSCs...... by ectopic expression of the RNA binding protein LIN28B, a key regulator of fetal hematopoiesis, and this coincided with the clonal reversal to fetal-like elevated self-renewal and repopulation potential. These results anchor the attenuation of B-1a cell output to fetal HSC behavior and demonstrate...

  19. Antioxidant action of SMe1EC2, the low-basicity derivative of the pyridoindole stobadine, in cell free chemical models and at cellular level.

    Science.gov (United States)

    Balcerczyk, Aneta; Bartosz, Grzegorz; Drzewinska, Joanna; Piotrowski, Łukasz; Pulaski, Łukasz; Stefek, Milan

    2014-03-01

    The aim of the study was to evaluate the antioxidant action of SMe1EC2, the structural analogue of the hexahydropyridoindole antioxidant stobadine. The antiradical activity of SMe1EC2 was found to be higher when compared to stobadine, as determined both in cell-free model systems of AAPH-induced oxidation of dihydrorhodamine 123 and 2',7'-dichloro-dihydrofluorescein diacetate, and in the cellular system of stimulated macrophages RAW264.7. Analysis of proliferation of HUVEC and HUVEC-ST cells revealed absence of cytotoxic effect of SMe1EC2 at concentrations below 100 µM. The antioxidant activity of SMe1EC2, superior to the parent drug stobadine, is accounted for by both the higher intrinsic free radical scavenging action and by the better bioavailability of the low-basicity SMe1EC2 relative to the high-basicity stobadine.

  20. Environment Aware Cellular Networks

    KAUST Repository

    Ghazzai, Hakim

    2015-02-01

    The unprecedented rise of mobile user demand over the years have led to an enormous growth of the energy consumption of wireless networks as well as the greenhouse gas emissions which are estimated currently to be around 70 million tons per year. This significant growth of energy consumption impels network companies to pay huge bills which represent around half of their operating expenditures. Therefore, many service providers, including mobile operators, are looking for new and modern green solutions to help reduce their expenses as well as the level of their CO2 emissions. Base stations are the most power greedy element in cellular networks: they drain around 80% of the total network energy consumption even during low traffic periods. Thus, there is a growing need to develop more energy-efficient techniques to enhance the green performance of future 4G/5G cellular networks. Due to the problem of traffic load fluctuations in cellular networks during different periods of the day and between different areas (shopping or business districts and residential areas), the base station sleeping strategy has been one of the main popular research topics in green communications. In this presentation, we present several practical green techniques that provide significant gains for mobile operators. Indeed, combined with the base station sleeping strategy, these techniques achieve not only a minimization of the fossil fuel consumption but also an enhancement of mobile operator profits. We start with an optimized cell planning method that considers varying spatial and temporal user densities. We then use the optimal transport theory in order to define the cell boundaries such that the network total transmit power is reduced. Afterwards, we exploit the features of the modern electrical grid, the smart grid, as a new tool of power management for cellular networks and we optimize the energy procurement from multiple energy retailers characterized by different prices and pollutant

  1. Engineering Cellular Metabolism

    DEFF Research Database (Denmark)

    Nielsen, Jens; Keasling, Jay

    2016-01-01

    of metabolic engineering and will discuss how new technologies can enable metabolic engineering to be scaled up to the industrial level, either by cutting off the lines of control for endogenous metabolism or by infiltrating the system with disruptive, heterologous pathways that overcome cellular regulation.......Metabolic engineering is the science of rewiring the metabolism of cells to enhance production of native metabolites or to endow cells with the ability to produce new products. The potential applications of such efforts are wide ranging, including the generation of fuels, chemicals, foods, feeds...

  2. Cellular: Toward personal communications

    Science.gov (United States)

    Heffernan, Stuart

    1991-09-01

    The cellular industry is one of the fastest growing segment of the telecommunications industry. With an estimated penetration rate of 20 percent in the near future, cellular is becoming an ubiquitous telecommunications service in the U.S. In this paper we will examine the major advancements in the cellular industry: customer equipment, cellular networks, engineering tools, customer support, and nationwide seamless service.

  3. Elucidation of lead-induced oxidative stress in Talinum triangulare roots by analysis of antioxidant responses and DNA damage at cellular level.

    Science.gov (United States)

    Kumar, Abhay; Prasad, M N V; Mohan Murali Achary, V; Panda, Brahma B

    2013-07-01

    Hydroponic experiments were performed with Talinum triangulare (Jacq.) Willd. focusing the root cellular biochemistry with special emphasis on DNA damage, structural, and elemental analyses in Pb(NO3)2 exposed with 0, 0.25, 0.5, 0.75, 1.0, and 1.25 mM for 7 days. Lead (Pb) increased reactive oxygen species production, lipid peroxidation, protein oxidation, cell death, and DNA damage and decreased the protein content in a dose-dependent manner. Likewise, a dose-dependent induction of antioxidative enzymes superoxide dismutase and catalase by Pb was evident. Ascorbate peroxidase on the other hand responded biphasically to Pb treatments by showing induction at low (0.25 and 0.50) and repression at high (0.75-1.25 mM) concentrations. The estimation of proline content also indicated a similar biphasic trend. Scanning electron microscope and energy-dispersive X-ray spectroscopy analysis showed that 1.25 mM Pb treatment resulted in ultrastructural modifications in roots and stem tissue that was marked by the change in the elemental profile. The findings pointed to the role of oxidative stress in the underlying Pb phytotoxicity and genotoxicity in T. triangulare. PMID:23263755

  4. Molecular chemistry of plant protein structure at a cellular level by synchrotron-based FTIR spectroscopy: Comparison of yellow ( Brassica rapa) and Brown ( Brassica napus) canola seed tissues

    Science.gov (United States)

    Yu, Peiqiang

    2008-05-01

    The objective of this study was to use synchrotron light sourced FTIR microspectroscopy as a novel approach to characterize protein molecular structure of plant tissue: compared yellow and brown Brassica canola seed within cellular dimensions. Differences in the molecular chemistry and the structural-chemical characteristics were identified between two type of plant tissues. The yellow canola seeds contained a relatively lower (P < 0.05) percentage of model-fitted α-helices (33 vs. 37), a higher (P < 0.05) relative percentage of model-fitted β-sheets (27 vs. 21) and a lower (P < 0.05) ratio of α-helices to β-sheets (1.3 vs. 1.9) than the brown seeds. These results may indicate that the protein value of the yellow canola seeds as food or feed was different from that of the brown canola seeds. The cluster analysis and principal component analysis did not show clear differences between the yellow and brown canola seed tissues in terms of protein amide I structures, indicating they are related to each other. Both yellow and brown canola seeds contain the same proteins but in different ratios.

  5. Effect of the sequential therapy of lamivudine and α-interferon on cellular immune function as well as serum PD-1 and Tin-3 levels in patients with chronic hepatitis B

    Institute of Scientific and Technical Information of China (English)

    Yan Jin; Ting Qiu; Yi-Fei Lyu; Chun-Ying Yan; Xue Wang; Tian-Jiao Duan; Rong Zhang; Gui-Sheng Liu

    2016-01-01

    Objective:To analyze the effect of the sequential therapy of lamivudine and α-interferon on cellular immune function as well as serum PD-1 and Tin-3 levels in patients with chronic hepatitis B. Methods: A total of 92 cases of patients with chronic hepatitis B who were treated in our hospital from May 2012 to May 2015 were selected as the research subjects and divided into observation group and control group (n=46) according to the random number table. Control group received lamivudine treatment alone, observation group received the sequential therapy of lamivudine and α-interferon, and then differences in ultrasound-related indexes, cellular immune function as well as PD-1 and Tin-3 levels were compared between two groups. Results:After observation group received the sequential therapy of lamivudine andα-interferon, ultrasonic major diameter of left hepatic lobe and PVM values were greater than those of control group, and internal diameter of portal vein was lower than that of control group; CD4+T and CD4+T/ CD8+T values of observation group were higher than those of control group, and CD8+T value was lower than that of control group;circulating blood CD8+T cell PD-1 and Tim-3 expression levels of observation group were lower than those of control group. Conclusion:Sequential therapy of lamivudine andα-interferon can optimize the cellular immune function of patients with chronic hepatitis B and inhibit the negative regulation process of immune function, and it helps to inhibit hepatitis B virus activity and disease control.

  6. In vitro expression levels of cell-cycle checkpoint proteins are associated with cellular DNA repair capacity in peripheral blood lymphocytes: a multivariate analysis

    OpenAIRE

    Fan, You-Hong; Hu, Zhibin; Li, Chunying; Wang, Li-E; Guo, Zhaozheng; Qiao, Yawei; Zhang, Li; Zhang, Wei; Mao, Li; Wei, Qingyi

    2007-01-01

    DNA repair should occur after cells sense DNA damage signals and undergo cell-cycle arrest to provide sufficient time for DNA repair, and suboptimal DNA repair capacity (DRC) in peripheral lymphocytes has been suggested as a cancer susceptibility marker. Numerous studies showed a functional link between DNA damage sensing, cell-cycle checkpoint and DNA repair. We hypothesized that in vitro cell-cycle checkpoint-related protein expression levels in stimulated lymphocytes predict DRC levels. To...

  7. Effect of electromagnetic fields at 2.45 GHz on the levels of cellular stress proteins HSP-90 and 70 in the rat thyroid; Efecto de los campos electromagneticos a 2,45 GHz sobre los niveles de proteinas de estres celular HSP-90 y 70 en el toroides de rata

    Energy Technology Data Exchange (ETDEWEB)

    Misa Agustino, M. J.; Alvarez-Folgueras, M.; Jorge-Mora, M. T.; Jorge Barreiro, F. J.; Ares Pena, F. J.; Lleiro, J.; Lopez Martin, M. E.

    2011-07-01

    In this study we analyzed the cellular stress levels achieved by heat shock proteins (HSP) 90 and 70 in rat thyroid tissue after exposure to radio waves in TWG experimental system. Parallel measurements of body stress in animals by rectal temperature probes allow us to determine whether there is any interaction between temperature increases and cellular stress.

  8. a Euclidean Formulation of Interior Orientation Costraints Imposed by the Fundamental Matrix

    Science.gov (United States)

    Kalisperakis, I.; Karras, G.; Petsa, E.

    2016-06-01

    Epipolar geometry of a stereopair can be expressed either in 3D, as the relative orientation (i.e. translation and rotation) of two bundles of optical rays in case of calibrated cameras or, in case of unclalibrated cameras, in 2D as the position of the epipoles on the image planes and a projective transformation that maps points in one image to corresponding epipolar lines on the other. The typical coplanarity equation describes the first case; the Fundamental matrix describes the second. It has also been proven in the Computer Vision literature that 2D epipolar geometry imposes two independent constraints on the parameters of camera interior orientation. In this contribution these constraints are expressed directly in 3D Euclidean space by imposing the equality of the dihedral angle of epipolar planes defined by the optical axes of the two cameras or by suitably chosen corresponding epipolar lines. By means of these constraints, new closed form algorithms are proposed for the estimation of a variable or common camera constant value given the fundamental matrix and the principal point position of a stereopair.

  9. Color Image Quantization Based on Euclidean Distance Using Bacteria Foraging Optimization

    Directory of Open Access Journals (Sweden)

    Heena

    2012-09-01

    Full Text Available The RGB color model is an additive color model that yields a broad array of colors in which three primary colors red, green and blue are added together in various ways.RGB is device dependent color model used in input devices like color TV and video cameras, image scanners etc. and output devices like mobile phone displays, LCD etc. Bacteria Foraging Optimization is a nature-inspired optimization has drawn the attention of researchers because of its efficiency in solving real-world optimization problems arising in several application domains. Color image quantization is an important process of representing true color image using a small number of colors. The objective of this research work are to; 1 implement and compare its performance with other quantization techniques. To test the performance of proposed algorithm different quantative parameters like Quantized Distance, LMSE, Euclidean distance are used. 2 The results indicate that the proposed algorithm yields a significant improvement in image quality as compared to other approaches.

  10. Failure mode and effects analysis using intuitionistic fuzzy hybrid weighted Euclidean distance operator

    Science.gov (United States)

    Liu, Hu-Chen; Liu, Long; Li, Ping

    2014-10-01

    Failure mode and effects analysis (FMEA) has shown its effectiveness in examining potential failures in products, process, designs or services and has been extensively used for safety and reliability analysis in a wide range of industries. However, its approach to prioritise failure modes through a crisp risk priority number (RPN) has been criticised as having several shortcomings. The aim of this paper is to develop an efficient and comprehensive risk assessment methodology using intuitionistic fuzzy hybrid weighted Euclidean distance (IFHWED) operator to overcome the limitations and improve the effectiveness of the traditional FMEA. The diversified and uncertain assessments given by FMEA team members are treated as linguistic terms expressed in intuitionistic fuzzy numbers (IFNs). Intuitionistic fuzzy weighted averaging (IFWA) operator is used to aggregate the FMEA team members' individual assessments into a group assessment. IFHWED operator is applied thereafter to the prioritisation and selection of failure modes. Particularly, both subjective and objective weights of risk factors are considered during the risk evaluation process. A numerical example for risk assessment is given to illustrate the proposed method finally.

  11. Shallow, Low, and Light Trees, and Tight Lower Bounds for Euclidean Spanners

    CERN Document Server

    Dinitz, Yefim; Solomon, Shay

    2008-01-01

    We show that for every $n$-point metric space $M$ there exists a spanning tree $T$ with unweighted diameter $O(\\log n)$ and weight $\\omega(T) = O(\\log n) \\cdot \\omega(MST(M))$. Moreover, there is a designated point $rt$ such that for every point $v$, $dist_T(rt,v) \\le (1+\\epsilon) \\cdot dist_M(rt,v)$, for an arbitrarily small constant $\\epsilon > 0$. We extend this result, and provide a tradeoff between unweighted diameter and weight, and prove that this tradeoff is \\emph{tight up to constant factors} in the entire range of parameters. These results enable us to settle a long-standing open question in Computational Geometry. In STOC'95 Arya et al. devised a construction of Euclidean Spanners with unweighted diameter $O(\\log n)$ and weight $O(\\log n) \\cdot \\omega(MST(M))$. Ten years later in SODA'05 Agarwal et al. showed that this result is tight up to a factor of $O(\\log \\log n)$. We close this gap and show that the result of Arya et al. is tight up to constant factors.

  12. The Exact Euclidean Distance Transform: A New Algorithm for Universal Path Planning

    Directory of Open Access Journals (Sweden)

    Juan Carlos Elizondo-Leal

    2013-06-01

    Full Text Available The Path‐Planning problem is a basic issue in mobile robotics, in order to allow the robots to solve more complex tasks, for example, an exploration assignment in which the distance given by the planner is taken as a utility measure. Among the different proposed approaches, algorithms based on an exact cell decomposition of the environment are very popular. In this paper, we present a new algorithm for universal path planning in cell decomposition, using a raster scan method for computing the Exact Euclidean Distance Transform (EEDT for each cell in the map. Our algorithm computes, for every cell in the map, the point sequence to the goal. For each sequence, the sub‐goals are selected near to the vertices of the obstacles, reducing the total distance to the goal without post processing. At the end, we obtain a smooth path up to the goal without the need for post‐processing. The paths are computed by visibility verification among the cells, exploiting the processing performed in the neighbouring cells.

  13. New formulae for the Hubble Constant in a Euclidean Static Universe

    CERN Document Server

    Lorenzo, Zaninetti

    2010-01-01

    It is shown that the Hubble constant can be derived from the standard luminosity function of galaxies as well as from a new luminosity function as deduced from the mass-luminosity relationship for galaxies. An analytical expression for the Hubble constant can be found from the maximum number of galaxies (in a given solid angle and flux) as a function of the redshift. A second analytical definition of the Hubble constant can be found from the redshift averaged over a given solid angle and flux. The analysis of two luminosity functions for galaxies brings to four the new definitions of the Hubble constant. The equation that regulates the Malmquist bias for galaxies is derived and as a consequence it is possible to extract a complete sample. The application of these new formulae to the data of the two-degree Field Galaxy Redshift Survey provides a Hubble constant of $( 65.26 \\pm 8.22 ) \\mathrm{\\ km\\ s}^{-1}\\mathrm{\\ Mpc}^{-1}$ for a redshift lower than 0.042. All the results are deduced in a Euclidean universe b...

  14. Bayesian approach to spectral function reconstruction for Euclidean quantum field theories.

    Science.gov (United States)

    Burnier, Yannis; Rothkopf, Alexander

    2013-11-01

    We present a novel approach to the inference of spectral functions from Euclidean time correlator data that makes close contact with modern Bayesian concepts. Our method differs significantly from the maximum entropy method (MEM). A new set of axioms is postulated for the prior probability, leading to an improved expression, which is devoid of the asymptotically flat directions present in the Shanon-Jaynes entropy. Hyperparameters are integrated out explicitly, liberating us from the Gaussian approximations underlying the evidence approach of the maximum entropy method. We present a realistic test of our method in the context of the nonperturbative extraction of the heavy quark potential. Based on hard-thermal-loop correlator mock data, we establish firm requirements in the number of data points and their accuracy for a successful extraction of the potential from lattice QCD. Finally we reinvestigate quenched lattice QCD correlators from a previous study and provide an improved potential estimation at T=2.33T(C). PMID:24237510

  15. New results on embeddings of polyhedra and manifolds in Euclidean spaces

    Energy Technology Data Exchange (ETDEWEB)

    Repovs, D [University of Ljubljana (Slovenia); Skopenkov, A B [M.V. Lomonosov Moscow State University, Moscow (Russian Federation)

    1999-12-31

    The aim of this survey is to present several classical results on embeddings and isotopies of polyhedra and manifolds in R{sup m}. We also describe the revival of interest in this beautiful branch of topology and give an account of new results, including an improvement of the Haefliger-Weber theorem on the completeness of the deleted product obstruction to embeddability and isotopy of highly connected manifolds in R{sup m} (Skopenkov) as well as the unimprovability of this theorem for polyhedra (Freedman, Krushkal, Teichner, Segal, Skopenkov, and Spiez) and for manifolds without the necessary connectedness assumption (Skopenkov). We show how algebraic obstructions (in terms of cohomology, characteristic classes, and equivariant maps) arise from geometric problems of embeddability in Euclidean spaces. Several classical and modern results on completeness or incompleteness of these obstructions are stated and proved. By these proofs we illustrate classical and modern tools of geometric topology (engulfing, the Whitney trick, van Kampen and Casson finger moves, and their generalizations)

  16. Euclidean geodesic loops on high-genus surfaces applied to the morphometry of vestibular systems.

    Science.gov (United States)

    Xin, Shi-Qing; He, Ying; Fu, Chi-Wing; Wang, Defeng; Lin, Shi; Chu, Winnie C W; Cheng, Jack C Y; Gu, Xianfeng; Lui, Lok Ming

    2011-01-01

    This paper proposes a novel algorithm to extract feature landmarks on the vestibular system (VS), for the analysis of Adolescent Idiopathic Scoliosis (AIS) disease. AIS is a 3-D spinal deformity commonly occurred in adolescent girls with unclear etiology. One popular hypothesis was suggested to be the structural changes in the VS that induce the disturbed balance perception, and further cause the spinal deformity. The morphometry of VS to study the geometric differences between the healthy and AIS groups is of utmost importance. However, the VS is a genus-3 structure situated in the inner ear. The high-genus topology of the surface poses great challenge for shape analysis. In this work, we present a new method to compute exact geodesic loops on the VS. The resultant geodesic loops are in Euclidean metric, thus characterizing the intrinsic geometric properties of the VS based on the real background geometry. This leads to more accurate results than existing methods, such as the hyperbolic Ricci flow method. Furthermore, our method is fully automatic and highly efficient, e.g., one order of magnitude faster than. We applied our algorithm to the VS of normal and AIS subjects. The promising experimental results demonstrate the efficacy of our method and reveal more statistically significant shape difference in the VS between right-thoracic AIS and normal subjects.

  17. Euclidean Dynamical Triangulation revisited: is the phase transition really 1st order? (extended version)

    CERN Document Server

    Rindlisbacher, Tobias

    2015-01-01

    The transition between the two phases of 4D Euclidean Dynamical Triangulation [1] was long believed to be of second order until in 1996 first order behavior was found for sufficiently large systems [5,9]. However, one may wonder if this finding was affected by the numerical methods used: to control volume fluctuations, in both studies [5,9] an artificial harmonic potential was added to the action; in [9] measurements were taken after a fixed number of accepted instead of attempted moves which introduces an additional error. Finally the simulations suffer from strong critical slowing down which may have been underestimated. In the present work, we address the above weaknesses: we allow the volume to fluctuate freely within a fixed interval; we take measurements after a fixed number of attempted moves; and we overcome critical slowing down by using an optimized parallel tempering algorithm [12]. With these improved methods, on systems of size up to 64k 4-simplices, we confirm that the phase transition is first ...

  18. Euclidean Dynamical Triangulation revisited: is the phase transition really first order?

    CERN Document Server

    Rindlisbacher, Tobias

    2014-01-01

    The transition between the two phases of 4D Euclidean Dynamical Triangulation [1] was long believed to be of second order until in 1996 first order behavior was found for sufficiently large systems [3,4]. However, one may wonder if this finding was affected by the numerical methods used: to control volume fluctuations, in both studies [3,4] an artificial harmonic potential was added to the action; in [4] measurements were taken after a fixed number of accepted instead of attempted moves which introduces an additional error. Finally the simulations suffer from strong critical slowing down which may have been underestimated. In the present work, we address the above weaknesses: we allow the volume to fluctuate freely within a fixed interval; we take measurements after a fixed number of attempted moves; and we overcome critical slowing down by using an optimized parallel tempering algorithm [6]. With these improved methods, on systems of size up to 64k 4-simplices, we confirm that the phase transition is first o...

  19. A PTAS For The k-Consensus Structures Problem Under Squared Euclidean Distance

    Directory of Open Access Journals (Sweden)

    Louxin Zhang

    2008-10-01

    Full Text Available In this paper we consider a basic clustering problem that has uses in bioinformatics. A structural fragment is a sequence of l points in a 3D space, where l is a fixed natural number. Two structural fragments f1 and f2 are equivalent if and only if f1 = f2 x R + τ under some rotation R and translation τ . We consider the distance between two structural fragments to be the sum of the squared Euclidean distance between all corresponding points of the structural fragments. Given a set of n structural fragments, we consider the problem of finding k (or fewer structural fragments g1, g2, ... , gk, so as to minimize the sum of the distances between each of f1, f2, ... , fn to its nearest structural fragment in g1, ... , gk. In this paper we show a polynomial-time approximation scheme (PTAS for the problem through a simple sampling strategy.

  20. Multi-resolutional brain network filtering and analysis via wavelets on non-Euclidean space.

    Science.gov (United States)

    Kim, Won Hwa; Adluru, Nagesh; Chung, Moo K; Charchut, Sylvia; GadElkarim, Johnson J; Altshuler, Lori; Moody, Teena; Kumar, Anand; Singh, Vikas; Leow, Alex D

    2013-01-01

    Advances in resting state fMRI and diffusion weighted imaging (DWI) have led to much interest in studies that evaluate hypotheses focused on how brain connectivity networks show variations across clinically disparate groups. However, various sources of error (e.g., tractography errors, magnetic field distortion, and motion artifacts) leak into the data, and make downstream statistical analysis problematic. In small sample size studies, such noise have an unfortunate effect that the differential signal may not be identifiable and so the null hypothesis cannot be rejected. Traditionally, smoothing is often used to filter out noise. But the construction of convolving with a Gaussian kernel is not well understood on arbitrarily connected graphs. Furthermore, there are no direct analogues of scale-space theory for graphs--ones which allow to view the signal at multiple resolutions. We provide rigorous frameworks for performing 'multi-resolutional' analysis on brain connectivity graphs. These are based on the recent theory of non-Euclidean wavelets. We provide strong evidence, on brain connectivity data from a network analysis study (structural connectivity differences in adult euthymic bipolar subjects), that the proposed algorithm allows identifying statistically significant network variations, which are clinically meaningful, where classical statistical tests, if applied directly, fail.

  1. Robust Automatic Pectoral Muscle Segmentation from Mammograms Using Texture Gradient and Euclidean Distance Regression.

    Science.gov (United States)

    Bora, Vibha Bafna; Kothari, Ashwin G; Keskar, Avinash G

    2016-02-01

    In computer-aided diagnosis (CAD) of mediolateral oblique (MLO) view of mammogram, the accuracy of tissue segmentation highly depends on the exclusion of pectoral muscle. Robust methods for such exclusions are essential as the normal presence of pectoral muscle can bias the decision of CAD. In this paper, a novel texture gradient-based approach for automatic segmentation of pectoral muscle is proposed. The pectoral edge is initially approximated to a straight line by applying Hough transform on Probable Texture Gradient (PTG) map of the mammogram followed by block averaging with the aid of approximated line. Furthermore, a smooth pectoral muscle curve is achieved with proposed Euclidean Distance Regression (EDR) technique and polynomial modeling. The algorithm is robust to texture and overlapping fibro glandular tissues. The method is validated with 340 MLO views from three databases-including 200 randomly selected scanned film images from miniMIAS, 100 computed radiography images and 40 full-field digital mammogram images. Qualitatively, 96.75 % of the pectoral muscles are segmented with an acceptable pectoral score index. The proposed method not only outperforms state-of-the-art approaches but also accurately quantifies the pectoral edge. Thus, its high accuracy and relatively quick processing time clearly justify its suitability for CAD.

  2. $O(N)$ model in Euclidean de Sitter space: beyond the leading infrared approximation

    CERN Document Server

    Nacir, Diana López; Trombetta, Leonardo G

    2016-01-01

    We consider an $O(N)$ scalar field model with quartic interaction in $d$-dimensional Euclidean de Sitter space. In order to avoid the problems of the standard perturbative calculations for light and massless fields, we generalize to the $O(N)$ theory a systematic method introduced previously for a single field, which treats the zero modes exactly and the nonzero modes perturbatively. We compute the two-point functions taking into account not only the leading infrared contribution, coming from the self-interaction of the zero modes, but also corrections due to the interaction of the ultraviolet modes. For the model defined in the corresponding Lorentzian de Sitter spacetime, we obtain the two-point functions by analytical continuation. We point out that a partial resummation of the leading secular terms (which necessarily involves nonzero modes) is required to obtain a decay at large distances for massless fields. We implement this resummation along with a systematic double expansion in an effective coupling c...

  3. Programs for kriging and sequential Gaussian simulation with locally varying anisotropy using non-Euclidean distances

    Science.gov (United States)

    Boisvert, Jeff B.; Deutsch, Clayton V.

    2011-04-01

    Geological deposits display nonlinear features such as veins, channels or folds that result in complex spatial anisotropies that are difficult to model with currently available geostatistical techniques. The methodology presented in this paper for incorporating locally varying anisotropy in kriging or sequential Gaussian simulation is based on modifying how locations in space are related. Normally, the straight line path is used; however, when nonlinear features exist the appropriate path between locations follows along the features. The Dijkstra algorithm is used to determine the shortest path/distance between locations and a conventional covariance or variogram function is used. This nonlinear path is a non-Euclidean distance metric and positive definiteness of the resulting kriging system of equations is not guaranteed. Multidimensional scaling (landmark isometric mapping) is used to ensure positive definiteness. In addition to the variogram, the only parameters required for the implementation of kriging or sequential Gaussian simulation with locally varying anisotropy are (1) the local orientation and magnitude of anisotropy and (2) the number of dimensions required for multidimensional scaling. This paper presents a suite of programs that can be used to krige or simulate practically sized geostatistical models with locally varying anisotropy. The programs kt3d_LVA, SGS_LVA and gamv_LVA are provided.

  4. Correlations and clustering in a scale-free network in Euclidean space

    Indian Academy of Sciences (India)

    A K Nandi; G Mukherjee; S S Manna

    2008-08-01

    Empirical study shows that many real networks in nature and society share two generic properties: they are scale-free and they display a high degree of clustering. Quite often they are modular in nature also, implying occurrences of several small tightly linked groups which are connected in a hierarchical manner among themselves. Recently, we have introduced a model of spatial scale-free network where nodes pop-up at randomly located positions in the Euclidean space and are connected to one end of the nearest link of the existing network. It has been already argued that the large scale behaviour of this network is like the Barabási–Albert model. In the present paper we briefly review these results as well as present additional results on the study of non-trivial correlations present in this model which are found to have similar behaviours as in the real-world networks. Moreover, this model naturally possesses the hierarchical characteristics lacked by most of the models of the scale-free networks.

  5. Face Detection and Recognition Using Viola-Jones with PCA-LDA and Square Euclidean Distance

    Directory of Open Access Journals (Sweden)

    Nawaf Hazim Barnouti

    2016-05-01

    Full Text Available In this paper, an automatic face recognition system is proposed based on appearance-based features that focus on the entire face image rather than local facial features. The first step in face recognition system is face detection. Viola-Jones face detection method that capable of processing images extremely while achieving high detection rates is used. This method has the most impact in the 2000’s and known as the first object detection framework to provide relevant object detection that can run in real time. Feature extraction and dimension reduction method will be applied after face detection. Principal Component Analysis (PCA method is widely used in pattern recognition. Linear Discriminant Analysis (LDA method that used to overcome drawback the PCA has been successfully applied to face recognition. It is achieved by projecting the image onto the Eigenface space by PCA after that implementing pure LDA over it. Square Euclidean Distance (SED is used. The distance between two images is a major concern in pattern recognition. The distance between the vectors of two images leads to image similarity. The proposed method is tested on three databases (MUCT, Face94, and Grimace. Different number of training and testing images are used to evaluate the system performance and it show that increasing the number of training images will increase the recognition rate.

  6. A NOVEL CONSTRUCTION OF QUANTUM LDPC CODES BASED ON CYCLIC CLASSES OF LINES IN EUCLIDEAN GEOMETRIES

    Institute of Scientific and Technical Information of China (English)

    Cao Dong; Song Yaoliang; Zhao Shengmei

    2012-01-01

    The dual-containing (or self-orthogonal) formalism of Calderbank-Shor-Steane (CSS) codes provides a universal connection between a classical linear code and a Quantum Error-Correcting Code (QECC).We propose a novel class of quantum Low Density Parity Check (LDPC) codes constructed from cyclic classes of lines in Euclidean Geometry (EG).The corresponding constructed parity check matrix has quasi-cyclic structure that can be encoded flexibility,and satisfies the requirement of dual-containing quantum code.Taking the advantage of quasi-cyclic structure,we use a structured approach to construct Generalized Parity Check Matrix (GPCM).This new class of quantum codes has higher code rate,more sparse check matrix,and exactly one four-cycle in each pair of two rows.Experimental results show that the proposed quantum codes,such as EG(2,q)Ⅱ-QECC,EG(3,q)Ⅱ-QECC,have better performance than that of other methods based on EG,over the depolarizing channel and decoded with iterative decoding based on the sum-product decoding algorithm.

  7. The role of dose rate in radiation cancer risk: evaluating the effect of dose rate at the molecular, cellular and tissue levels using key events in critical pathways following exposure to low LET radiation

    Science.gov (United States)

    Brooks, Antone L.; Hoel, David G.; Preston, R. Julian

    2016-01-01

    Abstract Purpose: This review evaluates the role of dose rate on cell and molecular responses. It focuses on the influence of dose rate on key events in critical pathways in the development of cancer. This approach is similar to that used by the U.S. EPA and others to evaluate risk from chemicals. It provides a mechanistic method to account for the influence of the dose rate from low-LET radiation, especially in the low-dose region on cancer risk assessment. Molecular, cellular, and tissues changes are observed in many key events and change as a function of dose rate. The magnitude and direction of change can be used to help establish an appropriate dose rate effectiveness factor (DREF). Conclusions: Extensive data on key events suggest that exposure to low dose-rates are less effective in producing changes than high dose rates. Most of these data at the molecular and cellular level support a large (2–30) DREF. In addition, some evidence suggests that doses delivered at a low dose rate decrease damage to levels below that observed in the controls. However, there are some data human and mechanistic data that support a dose-rate effectiveness factor of 1. In summary, a review of the available molecular, cellular and tissue data indicates that not only is dose rate an important variable in understanding radiation risk but it also supports the selection of a DREF greater than one as currently recommended by ICRP (2007) and BEIR VII (NRC/NAS 2006). PMID:27266588

  8. Identification and prioritization of candidate genes for symptom variability in breast cancer survivors based on disease characteristics at the cellular level

    Directory of Open Access Journals (Sweden)

    Koleck TA

    2016-03-01

    Full Text Available Theresa A Koleck,1 Yvette P Conley2 1School of Nursing, 2Department of Human Genetics, School of Nursing and Graduate School of Public Health, University of Pittsburgh, Pittsburgh, PA, USA Abstract: Research is beginning to suggest that the presence and/or severity of symptoms reported by breast cancer survivors may be associated with disease-related factors of cancer. In this article, we present a novel approach to the identification and prioritization of biologically plausible candidate genes to investigate relationships between genomic variation and symptom variability in breast cancer survivors. Cognitive dysfunction is utilized as a representative breast cancer survivor symptom to elucidate the conceptualization of and justification for our cellular, disease-based approach to address symptom variability in cancer survivors. Initial candidate gene identification was based on genes evaluated as part of multigene expression profiles for breast cancer, which are commonly used in the clinical setting to characterize the biology of cancer cells for the purpose of describing overall tumor aggressiveness, prognostication, and individualization of therapy. A list of genes evaluated within five multigene expression profiles for breast cancer was compiled. In order to prioritize candidate genes for investigation, genes used in each profile were compared for duplication. Twenty-one genes (BAG1, BCL2, BIRC5, CCNB1, CENPA, CMC2, DIAPH3, ERBB2, ESR1, GRB7, MELK, MKI67, MMP11, MYBL2, NDC80, ORC6, PGR, RACGAP1, RFC4, RRM2, and SCUBE2 are utilized in two or more profiles, including five genes (CCNB1, CENPA, MELK, MYBL2, and ORC6 used in three profiles. To ensure that the parsimonious 21 gene set is representative of the more global biological hallmarks of cancer, an Ingenuity Pathway Analysis was conducted. Evaluation of genes known to impact pathways involved with cancer development and progression provide a means to evaluate the overlap between the

  9. Scaling laws for non-Euclidean plates and the $W^{2,2}$ isometric immersions of Riemannian metrics

    CERN Document Server

    Lewicka, Marta

    2009-01-01

    This paper concerns the elastic structures which exhibit non-zero strain at free equilibria. Many growing tissues (leaves, flowers or marine invertebrates) attain complicated configurations during their free growth. Our study departs from the 3d incompatible elasticity theory, conjectured to explain the mechanism for the spontaneous formation of non-Euclidean metrics. Recall that a smooth Riemannian metric on a simply connected domain can be realized as the pull-back metric of an orientation preserving deformation if and only if the associated Riemann curvature tensor vanishes identically. When this condition fails, one seeks a deformation yielding the closest metric realization. We set up a variational formulation of this problem by introducing the non-Euclidean version of the nonlinear elasticity functional, and establish its $\\Gamma$-convergence under the proper scaling. As a corollary, we obtain new necessary and sufficient conditions for existence of a $W^{2,2}$ isometric immersion of a given 2d metric i...

  10. Non-CMC Solutions to the Einstein Constraint Equations on Asymptotically Euclidean Manifolds with Apparent Horizon Boundaries

    CERN Document Server

    Holst, Michael

    2014-01-01

    In this article we further develop the solution theory for the Einstein constraint equations on an n-dimensional, asymptotically Euclidean manifold M with interior boundary S. Building on recent results for both the asymptotically Euclidean and compact with boundary settings, we show existence of far-from-CMC and near-CMC solutions to the conformal formulation of the Einstein constraints when nonlinear Robin boundary conditions are imposed on S, similar to those analyzed previously by Dain (2004), by Maxwell (2004, 2005), and by Holst and Tsogtgerel (2013) as a model of black holes in various CMC settings, and by Holst, Meier, and Tsogtgerel (2013) in the setting of far-from-CMC solutions on compact manifolds with boundary. These "marginally trapped surface" Robin conditions ensure that the expansion scalars along null geodesics perpendicular to the boundary region S are non-positive, which is considered the correct mathematical model for black holes in the context of the Einstein constraint equations. Assumi...

  11. Cordyceps militaris (L. Link Fruiting Body Reduces the Growth of a Non-Small Cell Lung Cancer Cell Line by Increasing Cellular Levels of p53 and p21

    Directory of Open Access Journals (Sweden)

    Ana Bizarro

    2015-07-01

    Full Text Available Cordyceps militaris (L. Link, an edible entomopathogenic fungus widely used in traditional Chinese medicine, has numerous potential medicinal properties including antitumor activity. The methanolic extract of C. militaris fruiting body was recently shown to have tumor cell growth inhibitory activity in several human tumor cell lines. Nonetheless, the mechanism of action involved is still not known. This work aimed at further studying the effect of the methanolic extract of C. militaris regarding its antitumor mechanism of action, using the non-small cell lung cancer cell line (NCI-H460 as a model. Results showed that treatment with the extract decreased cellular proliferation, induced cell cycle arrest at G0/G1 and increased apoptosis. In addition, the extract increased the levels of p53 and p21. Moreover, an increase in p-H2A.X and 53BP1 levels, together with an increase in the number of 53BP1 foci/cell (all indicative of DNA damage, were also observed after treatment with the extract. This work suggests that this extract affected NCI-H460 cellular viability through a mechanism involving DNA damage and p53 activation. This further supports the potential of this extract as a source of bioactive compounds, which may be used in anticancer strategies.

  12. Euclidean M-theory background dual to three-dimensional scale invariant field theory without conformal invariance

    CERN Document Server

    Nakayama, Yu

    2016-01-01

    We show that eleven dimensional supergravity in Euclidean signature admits an exact classical solution with isometry corresponding to a three dimensional scale invariant field theory without conformal invariance. We also construct the holographic renormalization group flow that connects the known UV conformal fixed point and the new scale invariant but not conformal fixed point. In view of holography, the existence of such classical solutions suggests that the topologically twisted M2-brane gauge theory possesses a scale invariant but not conformal phase.

  13. On CP1 and CP2 maps and Weierstrass representations for surfaces immersed into multi-dimensional Euclidean spaces

    OpenAIRE

    Grundland, A. M.; Zakrzewski, W. J.

    2003-01-01

    An extension of the classic Enneper-Weierstrass representation for conformally parametrised surfaces in multi-dimensional spaces is presented. This is based on low dimensional CP^1 and CP^2 sigma models which allow the study of the constant mean curvature (CMC) surfaces immersed into Euclidean 3- and 8-dimensional spaces, respectively. Relations of Weierstrass type systems to the equations of these sigma models are established. In particular, it is demonstrated that the generalised Weierstras...

  14. Comparative assessment of the recognition of domain-specific CD163 monoclonal antibodies in human monocytes explains wide discrepancy in reported levels of cellular surface CD163 expression

    DEFF Research Database (Denmark)

    Maniecki, Maciej Bogdan; Etzerodt, Anders; Moestrup, Søren Kragh;

    2011-01-01

    continue to exhibit great discrepancy in the measured percentage of CD163-expressing blood monocytes in healthy individuals. In this study we sought to clarify this inconsistency in reported levels of CD163 surface expression by a detailed analysis of a panel of CD163 antibodies used in previous studies......, and the specificity of the CD163 monoclonal antibodies was analyzed by western blotting. Results and Discussion Flow cytometric analysis revealed that the estimated proportion of CD163-expressing human peripheral blood monocytes increased when using CD163 monoclonal antibodies recognizing epitopes in the N...... of binding in heparin-stabilized whole blood observed by flow cytometry. In contrast, RM3/1 exhibited weak binding to CD163 in the absence of calcium but high affinity binding to CD163 in the presence of calcium. R-20 and MAC2-158 were unaffected by extracellular calcium levels. The latter SRCR domain 1 m...

  15. Experimental study of Americium-241 biokinetics in Homarus Gammarus lobster. Analysis of the accumulation and detoxication mechanisms at the sub-cellular level

    International Nuclear Information System (INIS)

    The Americium 241 radioelement accumulation and elimination rate and mechanisms in the lobster organism have been experimentally studied; incorporation and detoxification capacities of each organ are evaluated. The existence of various biological compartments is shown; the major role of the digestive gland in accumulation of the radioelement, its distribution towards the various organs, and its resorption is comprehensively described, with an analysis at the subcellular and molecular levels. 401 p., 65 fig., 43 tab., 428 ref

  16. Pose Estimation using 1D Fourier Transform and Euclidean Distance Matching of CAD Model and Inspected Model Part

    Science.gov (United States)

    Zulkoffli, Zuliani; Abu Bakar, Elmi

    2016-02-01

    This paper present pose estimation relation of CAD model object and Projection Real Object (PRI). Image sequence of PRI and CAD model rotate on z axis at 10 degree interval in simulation and real scene used in this experiment. All this image is go through preprocessing stage to rescale object size and image size and transform all the image into silhouette. Correlation of CAD and PRI image is going through in this stage. Magnitude spectrum shows a reliable value in range 0.99 to 1.00 and Phase spectrum correlation shows a fluctuate graph in range 0.56 - 0.97. Euclidean distance correlation graph for CAD and PRI shows 2 zone of similar value due to almost symmetrical object shape. Processing stage of retrieval inspected PRI image in CAD database was carried out using range phase spectrum and maximum magnitude spectrum value within ±10% tolerance. Additional processing stage of retrieval inspected PRI image using Euclidean distance within ±5% tolerance also carried out. Euclidean matching shows a reliable result compared to range phase spectrum and maximum magnitude spectrum value by sacrificing more than 5 times processing time.

  17. Soft learning vector quantization and clustering algorithms based on non-Euclidean norms: single-norm algorithms.

    Science.gov (United States)

    Karayiannis, Nicolaos B; Randolph-Gips, Mary M

    2005-03-01

    This paper presents the development of soft clustering and learning vector quantization (LVQ) algorithms that rely on a weighted norm to measure the distance between the feature vectors and their prototypes. The development of LVQ and clustering algorithms is based on the minimization of a reformulation function under the constraint that the generalized mean of the norm weights be constant. According to the proposed formulation, the norm weights can be computed from the data in an iterative fashion together with the prototypes. An error analysis provides some guidelines for selecting the parameter involved in the definition of the generalized mean in terms of the feature variances. The algorithms produced from this formulation are easy to implement and they are almost as fast as clustering algorithms relying on the Euclidean norm. An experimental evaluation on four data sets indicates that the proposed algorithms outperform consistently clustering algorithms relying on the Euclidean norm and they are strong competitors to non-Euclidean algorithms which are computationally more demanding.

  18. Examination of the Staphylococcus aureus nitric oxide reductase (saNOR) reveals its contribution to modulating intracellular NO levels and cellular respiration.

    Science.gov (United States)

    Lewis, A M; Matzdorf, S S; Endres, J L; Windham, I H; Bayles, K W; Rice, K C

    2015-05-01

    Staphylococcus aureus nitrosative stress resistance is due in part to flavohemoprotein (Hmp). Although hmp is present in all sequenced S. aureus genomes, 37% of analyzed strains also contain nor, encoding a predicted quinol-type nitric oxide (NO) reductase (saNOR). DAF-FM staining of NO-challenged wild-type, nor, hmp and nor hmp mutant biofilms suggested that Hmp may have a greater contribution to intracellular NO detoxification relative to saNOR. However, saNOR still had a significant impact on intracellular NO levels and complemented NO detoxification in a nor hmp mutant. When grown as NO-challenged static (low-oxygen) cultures, hmp and nor hmp mutants both experienced a delay in growth initiation, whereas the nor mutant's ability to initiate growth was comparable with the wild-type strain. However, saNOR contributed to cell respiration in this assay once growth had resumed, as determined by membrane potential and respiratory activity assays. Expression of nor was upregulated during low-oxygen growth and dependent on SrrAB, a two-component system that regulates expression of respiration and nitrosative stress resistance genes. High-level nor promoter activity was also detectable in a cell subpopulation near the biofilm substratum. These results suggest that saNOR contributes to NO-dependent respiration during nitrosative stress, possibly conferring an advantage to nor+ strains in vivo. PMID:25651868

  19. Two thoughts causing the birth of Non-Euclidean geometry%非欧几何诞生的两种思想

    Institute of Scientific and Technical Information of China (English)

    牟金保

    2011-01-01

    目的 系统分析和探讨非欧几何诞生的两种思想.方法 运用文献考证和历史分析的方法对非欧几何诞生的两种思想进行研究.结果 非欧几何诞生的两种重要思想分别是欧氏第五公设研究和运用微分几何研究;前者利用无模型观点直观揭示了非欧实质,后者以模型为依据不但利于人们理解和接受,而且产生了更一般的非欧几何.结论 两种思想研究的结合,使非欧几何真正诞生和快速发展,实现了非欧几何与欧氏几何的统一,为空间概念的扩展创造了必要条件.%Aim To analyze and discuss systematically two thoughts causing the birth of Non-Euclidean geometry. Methods Literature reviewed and historic analysis. Results The two important thoughts causing the birth of Non-Euclidean geometry were the fifth axiom research of Euclidean and the application of Differential geometry research; the former,from free-model point of view reveals the essence of Non-Euclidean geometry, the latter, based on models , not only promotes people to understand and accept it but also generates a more general Non-Euclidean geometry. Conclusion The combination of two thoughts makes the Non-Euclidean geometry emerge and develop rapidly, achieves the unity of Non-Euclidean geometry and Euclidean geometry and creates necessary conditions for the extension of space concept.

  20. Early Response Roles for Prolactin Cortisol and Circulating and Cellular Levels of Heat Shock Proteins 72 and 90α in Severe Sepsis and SIRS

    Directory of Open Access Journals (Sweden)

    K. Vardas

    2014-01-01

    Full Text Available Objective. To evaluate the early heat shock protein (HSP and hormonal stress response of intensive care unit (ICU patients with severe sepsis/septic shock (SS or systemic inflammatory response syndrome (SIRS compared to healthy subjects (H. Methods. Patients with early (first 48 hrs SS (n=29 or SIRS (n=29 admitted to a university ICU and 16 H were enrolled in the study. Serum prolactin, cortisol, and plasma ACTH were determined using immunoassay analyzers. ELISA was used to evaluate extracellular HSPs (eHSP90α, eHSP72 and interleukins. Mean fluorescence intensity (MFI values for intracellular HSPs (iHSP72, iHSP90α were measured using 4-colour flow-cytometry. Results. Prolactin, cortisol, and eHSP90α levels were significantly increased in SS patients compared to SIRS and H (P<0.003. ACTH and eHSP72 were significantly higher in SS and SIRS compared to H (P<0.005. SS monocytes expressed lower iHSP72 MFI levels compared to H (P=0.03. Prolactin was related with SAPS III and APACHE II scores and cortisol with eHSP90α, IL-6, and lactate (P<0.05. In SS and SIRS eHSP90α was related with eHSP72, IL-6, and IL-10. Conclusion. Prolactin, apart from cortisol, may have a role in the acute stress response in severe sepsis. In this early-onset inflammatory process, cortisol relates to eHSP90α, monocytes suppress iHSP72, and plasma eHSP72 increases.

  1. Multipartite entanglement arising from dense Euclidean lattices in dimensions 4-24

    International Nuclear Information System (INIS)

    The group of automorphisms of Euclidean (embedded in Rn) dense lattices such as the root lattices D4 and E8, the Barnes-Wall lattice BW16, the unimodular lattice D12+ and the Leech lattice Λ24 may be generated by entangled quantum gates of the corresponding dimension. These (real) gates/lattices are useful for quantum error correction: for instance, the two- and four-qubit real Clifford groups are the automorphism groups of the lattices D4 and BW16, respectively, and the three-qubit real Clifford group is maximal in the Weyl group W(E8). Technically, the automorphism group Aut(Λ) of the lattice Λ is the set of orthogonal matrices B such that, following the conjugation action by the generating matrix of the lattice, the output matrix is unimodular (of determinant ±1, with integer entries). When the degree n is equal to the number of basis elements of Λ, Aut(Λ) also acts on basis vectors and is generated with matrices B such that the sum of squared entries in a row is 1, i.e. B may be seen as a quantum gate. For the dense lattices listed above, maximal multipartite entanglement arises. In particular, one finds a balanced tripartite entanglement in E8 (the two- and three-tangles have the same magnitude 1/4) and a Greenberger-Horne-Zeilinger-type entanglement in BW16. In this paper, we also investigate the entangled gates from D12+ and Λ24, by seeing them as systems coupling a qutrit to two- and three-qubits, respectively. In addition to quantum computing, the work may be related to particle physics in the spirit of Planat et al (2011 Rep. Math. Phys. 66 39-51).

  2. Research Advances of Epilepsy on Cellular Level%癫痫病的细胞水平研究及进展

    Institute of Scientific and Technical Information of China (English)

    刘远琳; 何小华; 彭碧文

    2012-01-01

    Epilepsy is an incurable disorder of nervous system, and most treatments can only control the symptoms. The mechanism of epilepsy is still unknown. Many researchers try to learn the mechanism of epilepsy in different levels and by different methods, including molecular biology, cell and in vivo models. Technically, patch clamp, a functional method for electrophysiology, is an effective way to learn the characteristics and property of epilepsy. This review introduces the characteristics and application of cell preparation of patch clamp of epilepsy.%癫痫病是一种难治性神经系统疾病,目前医疗水平还不能将该病完全治愈,大多以控制为主.癫痫病的成因仍然未能阐明,很多学者针对其不同的成因,采用不同的方法来研究癫痫病的发作机制,研究层面也各不相同.技术手段上,膜片钳技术可以更加直接的从功能方面来阐述癫痫病电生理方面的特征与性质.此文主要探讨用于膜片钳技术的细胞材料的特点,以及其应用.

  3. Rapid Accumulation of Total Lipid in Rhizoclonium africanum Kutzing as Biodiesel Feedstock under Nutrient Limitations and the Associated Changes at Cellular Level

    Directory of Open Access Journals (Sweden)

    Gour Gopal Satpati

    2015-01-01

    Full Text Available Increase of total lipid and the proportion of the favorable fatty acids in marine green filamentous macroalga Rhizoclonium africanum (Chlorophyceae was studied under nitrate and phosphate limitations. These stresses were given by both eliminating and doubling the required amounts of nitrate and phosphate salts in the growth media. A significant twofold increase in total lipid (193.03 mg/g was achieved in cells in absence of nitrate in the culture medium, followed by phosphate limitation (142.65 mg/g. The intracellular accumulation of neutral lipids was observed by fluorescence microscopy. The scanning electron microscopic study showed the major structural changes under nutrient starvation. Fourier transform infrared spectroscopy (FTIR revealed the presence of ester (C-O-C stretching, ketone (C-C stretching, carboxylic acid (O-H bending, phosphine (P-H stretching, aromatic (C-H stretching and bending, and alcohol (O-H stretching and bending groups in the treated cells indicating the high accumulation of lipid hydrocarbons in the treated cells. Elevated levels of fatty acids favorable for biodiesel production, that is, C16:0, C16:1, C18:1, and C20:1, were identified under nitrate- and phosphate-deficient conditions. This study shows that the manipulation of cultural conditions could affect the biosynthetic pathways leading to increased lipid production while increasing the proportion of fatty acids suitable for biodiesel production.

  4. 中药细胞级粉碎技术在中药巴布剂中的应用分析%Application of Cellular Level Pulverizing Technology Used in Chinese Medicine Cataplasm

    Institute of Scientific and Technical Information of China (English)

    田宇光

    2014-01-01

    Objective:To explore the value of cellular level pulverizing technology used in cataplasm.Methods:Common fine and ultra-fine powder poultice were prepared,and the different sizes of cataplasma puhuang psorhamnetin-3-O-neohesperidin transdermal absorp-tion were comparatively analyzed.Results:The permeation rate of isorhamnetin-3-O-neohesperidin in ultrafine powder cataplasma was significantly higher than that in ordinary poultice (P<0.05 ).Conclusion:Cellular level pulverizing technology used in Chinese medi-cine preparations can help to improve the quality and bioavailability of drugs,optimize the production processes of Chinese medicine preparations and reduce drugs profligacy,and hence it deserves further application.%目的:研究中药细胞级粉碎技术应用于中药巴布剂的价值。方法:制备普通细粉巴布剂和超细粉巴布剂,比较分析不同粒径巴布剂中蒲黄异鼠李素-3-O-新橙皮苷透皮吸收性。结果:超细粉巴布剂中异鼠李素-3-O-新橙皮苷的渗透速率显著高于普通巴布剂(P<0.05)。结论:中药细胞级粉碎技术应用于中药药剂的制备,有利于提高药物的质量以及生物利用度,优化中药药剂生产流程,降低药物浪费,值得进一步推广应用。

  5. Biokinetics of a transuranic (238PU) and a rare earth element (152Eu) in the lobster (Homarus gammarus): transfer mechanisms (accumulation and detoxification) in organs and at the cellular level

    International Nuclear Information System (INIS)

    The work presented here is an experimental investigation of the biokinetics of transfer of a transuranic and a rare earth element (238Pu and 152Eu) in the lobster Homarus gammarus. The study of 238Pu biokinetics forms part of a wider framework of research concerning the transfer of transuranic elements in marine species, while the study of 152Eu is carried out with a view to supporting the analogy between the behaviour of transuranics and rare earths in living organisms. Exactly the same experimental protocol, based on techniques from various disciplines (biology, biochemistry and metrology), was used to Investigate the biokinetics of transfer of these two radionuclides. The Individual lobsters were radiolabelled by means of one-shot or chronic ingestion of spiked meals. As the first approach, the kinetics and transfer mechanisms were studied In whole animal samples and in different organs distinct series of pathways through the different organs were identified in the case of both radionuclides, being mainly linked to digestive processes induced by the meal as well as the transport function of hemo-lymph in this way, the Important role of the digestive gland was picked out, with two of four cellular types displaying a successive involvement In the fixation and then the retention of the studied radionuclides. As a corroborative approach, the digestive gland was subjected to a more detailed investigation with the aim of describing the mechanisms of Incorporation and elucidating transfer processes at the cellular and molecular levels. 238Pu is preferentially partitioned into the cytosol, where it is associated with various constituents such as ferritin (iron-storing protein). 152Eu is more diffusely distributed in the hepato-pancreatic cells, while the lysosomes appear to play a more important role during transfer. The results obtained in this study, both on the macroscopic scale (i.e. the whole animal and different organs) as well as the cellular and molecular levels

  6. Integrated cellular systems

    Science.gov (United States)

    Harper, Jason C.

    integrate cells and direct their behaviors. This process permits, for the first time, the selection and in situ isolation of a single target cell from a population of cells with mixed phenotypes, and the subsequent monitoring of its behavior, and that of its progeny, under well defined conditions. These techniques promise a new means to integrate biomolecules with nanostructures and macroscale systems, and to manipulate cellular behavior at the individual cell level, having significant implications towards development of practical and robust integrated cellular systems.

  7. Optimizing multi-resolution segmentation scale using empirical methods: Exploring the sensitivity of the supervised discrepancy measure Euclidean distance 2 (ED2)

    Science.gov (United States)

    Witharana, Chandi; Civco, Daniel L.

    2014-01-01

    Multiresolution segmentation (MRS) has proven to be one of the most successful image segmentation algorithms in the geographic object-based image analysis (GEOBIA) framework. This algorithm is relatively complex and user-dependent; scale, shape, and compactness are the main parameters available to users for controlling the algorithm. Plurality of segmentation results is common because each parameter may take a range of values within its parameter space or different combinations of values among parameters. Finding optimal parameter values through a trial-and-error process is commonly practiced at the expense of time and labor, thus, several alternative supervised and unsupervised methods for supervised automatic parameter setting have been proposed and tested. In the case of supervised empirical assessments, discrepancy measures are employed for computing measures of dissimilarity between a reference polygon and an image object candidate. Evidently the reliability of the optimal-parameter prediction heavily relies on the sensitivity of the segmentation quality metric. The idea behind pursuing optimal parameter setting is that, for instance, a given scale setting provides image object candidates different from the other scale setting; thus, by design the supervised quality metric should capture this difference. In this exploratory study, we selected the Euclidean distance 2 (ED2) metric, a recently proposed supervised metric, whose main design goal is to optimize the geometrical discrepancy (potential segmentation error (PSE)) and arithmetic discrepancy between image objects and reference polygons (number-of segmentation ratio (NSR)) in two dimensional Euclidean space, as a candidate to investigate the validity and efficacy of empirical discrepancy measures for finding the optimal scale parameter setting of the MRS algorithm. We chose test image scenes from four different space-borne sensors with varying spatial resolutions and scene contents and systematically

  8. Discovering New Drugs on the Cellular Level

    Science.gov (United States)

    2005-01-01

    With the Vision for Space Exploration calling for a sustained human presence in space, astronauts will need to grow plants, while in orbit, for nourishment that they will not receive from only consuming dehydrated foods. As a potential source of food for long-duration missions, space-grown plants could also give astronauts an important psychological boost, as fresh vegetables could serve as a welcomed change from monotonous meals consisting of reconstituted foods in plastic bags. Even more, these plants could likely aid in the recycling of air and wastewater on spacecraft. With a helping hand from a company by the name of Biolog, Inc., NASA is studying the impacts of decreased gravity and spaceborne bacteria on the plants being grown for food in space. With a helping hand from NASA, this very same company is creating powerful new cell- and bacteria-analysis tools for use in discovering and developing new drugs on Earth.

  9. Cellular-based sea level gauge

    Digital Repository Service at National Institute of Oceanography (India)

    Desai, R.G.P.; Joseph, A.

    telephone connections are severely susceptible to loss of connectivity during natural disasters such as storm surges, primarily because of telephone line breakage. Communication via VHF/UHF transceivers is limited by line-of-sight distance between... transceivers and normally offer only point-to-point data transfer. Satellite communication via platform transmit terminals (PTTs) has wide coverage and, therefore, allows data reception from offshore platforms. However, data transfer speeds are limited...

  10. Changes in rRNA levels during stress invalidates results from mRNA blotting: Fluorescence in situ rRNA hybridization permits renormalization for estimation of cellular mRNA levels

    DEFF Research Database (Denmark)

    Hansen, M.C.; Nielsen, A.K.; Molin, Søren;

    2001-01-01

    Regulation of gene expression can be analyzed by a number of different techniques. Some techniques monitor the level of specific mRNA directly, and others monitor indirectly by determining the level of enzymes encoded by the mRNA. Each method has its own inherent way of normalization. When results...... obtained by these techniques are compared between experiments in which differences in growth rates, strains, or stress treatments occur, the normalization procedure may have a significant impact on the results. In this report we present a solution to the normalization problem in RNA slot blotting...

  11. Polynomial degree reduction in the discrete L2-norm equals best Euclidean approximation of h-Bézier coefficients

    KAUST Repository

    Ait-Haddou, Rachid

    2015-06-04

    We show that the best degree reduction of a given polynomial P from degree n to m with respect to the discrete (Formula presented.)-norm is equivalent to the best Euclidean distance of the vector of h-Bézier coefficients of P from the vector of degree raised h-Bézier coefficients of polynomials of degree m. Moreover, we demonstrate the adequacy of h-Bézier curves for approaching the problem of weighted discrete least squares approximation. Applications to discrete orthogonal polynomials are also presented. © 2015 Springer Science+Business Media Dordrecht

  12. KDTREE 2: Fortran 95 and C++ software to efficiently search for near neighbors in a multi-dimensional Euclidean space

    OpenAIRE

    Kennel, Matthew B.

    2004-01-01

    Many data-based statistical algorithms require that one find \\textit{near or nearest neighbors} to a given vector among a set of points in that vector space, usually with Euclidean topology. The k-d data structure and search algorithms are the generalization of classical binary search trees to higher dimensional spaces, so that one may locate near neighbors to an example vector in $O(\\log N)$ time instead of the brute-force O(N) time, with $N$ being the size of the data base. KDTREE2 is a For...

  13. Deformed oscillator algebra for quantum superintegrable systems in two-dimensional Euclidean space and on a complex two-sphere

    International Nuclear Information System (INIS)

    In this work, we study superintegrable quantum systems in two-dimensional Euclidean space and on a complex two-sphere with second-order constants of motion. We show that these constants of motion satisfy the deformed oscillator algebra. Then, we easily calculate the energy eigenvalues in an algebraic way by solving of a system of two equations satisfied by its structure function. The results are in agreement to the ones obtained from the solution of the relevant Schrödinger equation

  14. Modelling cellular behaviour

    Science.gov (United States)

    Endy, Drew; Brent, Roger

    2001-01-01

    Representations of cellular processes that can be used to compute their future behaviour would be of general scientific and practical value. But past attempts to construct such representations have been disappointing. This is now changing. Increases in biological understanding combined with advances in computational methods and in computer power make it possible to foresee construction of useful and predictive simulations of cellular processes.

  15. Reversible quantum cellular automata

    CERN Document Server

    Schumacher, B

    2004-01-01

    We define quantum cellular automata as infinite quantum lattice systems with discrete time dynamics, such that the time step commutes with lattice translations and has strictly finite propagation speed. In contrast to earlier definitions this allows us to give an explicit characterization of all local rules generating such automata. The same local rules also generate the global time step for automata with periodic boundary conditions. Our main structure theorem asserts that any quantum cellular automaton is structurally reversible, i.e., that it can be obtained by applying two blockwise unitary operations in a generalized Margolus partitioning scheme. This implies that, in contrast to the classical case, the inverse of a nearest neighbor quantum cellular automaton is again a nearest neighbor automaton. We present several construction methods for quantum cellular automata, based on unitaries commuting with their translates, on the quantization of (arbitrary) reversible classical cellular automata, on quantum c...

  16. The role of sirtuins in cellular homeostasis.

    Science.gov (United States)

    Kupis, Wioleta; Pałyga, Jan; Tomal, Ewa; Niewiadomska, Ewa

    2016-09-01

    Sirtuins are evolutionarily conserved nicotinamide adenine dinucleotide (NAD(+))-dependent lysine deacylases or ADP-ribosyltransferases. These cellular enzymes are metabolic sensors sensitive to NAD(+) levels that maintain physiological homeostasis in the animal and plant cells. PMID:27154583

  17. Cellular bioluminescence imaging.

    Science.gov (United States)

    Welsh, David K; Noguchi, Takako

    2012-08-01

    Bioluminescence imaging of live cells has recently been recognized as an important alternative to fluorescence imaging. Fluorescent probes are much brighter than bioluminescent probes (luciferase enzymes) and, therefore, provide much better spatial and temporal resolution and much better contrast for delineating cell structure. However, with bioluminescence imaging there is virtually no background or toxicity. As a result, bioluminescence can be superior to fluorescence for detecting and quantifying molecules and their interactions in living cells, particularly in long-term studies. Structurally diverse luciferases from beetle and marine species have been used for a wide variety of applications, including tracking cells in vivo, detecting protein-protein interactions, measuring levels of calcium and other signaling molecules, detecting protease activity, and reporting circadian clock gene expression. Such applications can be optimized by the use of brighter and variously colored luciferases, brighter microscope optics, and ultrasensitive, low-noise cameras. This article presents a review of how bioluminescence differs from fluorescence, its applications to cellular imaging, and available probes, optics, and detectors. It also gives practical suggestions for optimal bioluminescence imaging of single cells.

  18. A Fuzzy Neural Network Based on Non-Euclidean Distance Clustering for Quality Index Model in Slashing Process

    Directory of Open Access Journals (Sweden)

    Yuxian Zhang

    2015-01-01

    Full Text Available The quality index model in slashing process is difficult to build by reason of the outliers and noise data from original data. To the above problem, a fuzzy neural network based on non-Euclidean distance clustering is proposed in which the input space is partitioned into many local regions by the fuzzy clustering based on non-Euclidean distance so that the computation complexity is decreased, and fuzzy rule number is determined by validity function based on both the separation and the compactness among clusterings. Then, the premise parameters and consequent parameters are trained by hybrid learning algorithm. The parameters identification is realized; meanwhile the convergence condition of consequent parameters is obtained by Lyapunov function. Finally, the proposed method is applied to build the quality index model in slashing process in which the experimental data come from the actual slashing process. The experiment results show that the proposed fuzzy neural network for quality index model has lower computation complexity and faster convergence time, comparing with GP-FNN, BPNN, and RBFNN.

  19. Heterogeneous cellular networks

    CERN Document Server

    Hu, Rose Qingyang

    2013-01-01

    A timely publication providing coverage of radio resource management, mobility management and standardization in heterogeneous cellular networks The topic of heterogeneous cellular networks has gained momentum in industry and the research community, attracting the attention of standardization bodies such as 3GPP LTE and IEEE 802.16j, whose objectives are looking into increasing the capacity and coverage of the cellular networks. This book focuses on recent progresses,  covering the related topics including scenarios of heterogeneous network deployment, interference management i

  20. Wavelet-based 3D reconstruction of microcalcification clusters from two mammographic views: new evidence that fractal tumors are malignant and Euclidean tumors are benign.

    Directory of Open Access Journals (Sweden)

    Kendra A Batchelder

    Full Text Available The 2D Wavelet-Transform Modulus Maxima (WTMM method was used to detect microcalcifications (MC in human breast tissue seen in mammograms and to characterize the fractal geometry of benign and malignant MC clusters. This was done in the context of a preliminary analysis of a small dataset, via a novel way to partition the wavelet-transform space-scale skeleton. For the first time, the estimated 3D fractal structure of a breast lesion was inferred by pairing the information from two separate 2D projected mammographic views of the same breast, i.e. the cranial-caudal (CC and mediolateral-oblique (MLO views. As a novelty, we define the "CC-MLO fractal dimension plot", where a "fractal zone" and "Euclidean zones" (non-fractal are defined. 118 images (59 cases, 25 malignant and 34 benign obtained from a digital databank of mammograms with known radiologist diagnostics were analyzed to determine which cases would be plotted in the fractal zone and which cases would fall in the Euclidean zones. 92% of malignant breast lesions studied (23 out of 25 cases were in the fractal zone while 88% of the benign lesions were in the Euclidean zones (30 out of 34 cases. Furthermore, a Bayesian statistical analysis shows that, with 95% credibility, the probability that fractal breast lesions are malignant is between 74% and 98%. Alternatively, with 95% credibility, the probability that Euclidean breast lesions are benign is between 76% and 96%. These results support the notion that the fractal structure of malignant tumors is more likely to be associated with an invasive behavior into the surrounding tissue compared to the less invasive, Euclidean structure of benign tumors. Finally, based on indirect 3D reconstructions from the 2D views, we conjecture that all breast tumors considered in this study, benign and malignant, fractal or Euclidean, restrict their growth to 2-dimensional manifolds within the breast tissue.

  1. Nanostructured cellular networks.

    Science.gov (United States)

    Moriarty, P; Taylor, M D R; Brust, M

    2002-12-01

    Au nanocrystals spin-coated onto silicon from toluene form cellular networks. A quantitative statistical crystallography analysis shows that intercellular correlations drive the networks far from statistical equilibrium. Spin-coating from hexane does not produce cellular structure, yet a strong correlation is retained in the positions of nanocrystal aggregates. Mechanisms based on Marangoni convection alone cannot account for the variety of patterns observed, and we argue that spinodal decomposition plays an important role in foam formation.

  2. Cellular Cardiomyoplasty: Clinical Application

    OpenAIRE

    Chachques, J. (J.); Acar, C; J. Herreros; Trainini, J. (Jorge); Prosper, F.; D’Attellis, N. (N.); Fabiani, J. N.; Carpentier, A

    2004-01-01

    Myocardial regeneration can be induced with the implantation of a variety of myogenic and angiogenic cell types. More than 150 patients have been treated with cellular cardiomyoplasty worldwide, 18 patients have been treated by our group. Cellular cardiomyoplasty seems to reduce the size and fibrosis of infarct scars, limit postischemic remodelling, and restore regional myocardial contractility. Techniques for skeletal myoblasts culture and ex vivo expansion using auto...

  3. Exact computation of the CDF of the Euclidean distance between a point and a random variable uniformly distributed in disks, balls, or polyhedrons and application to PSHA

    OpenAIRE

    Guigues, Vincent

    2014-01-01

    We consider a random variable expressed as the Euclidean distance between an arbitrary point and a random variable uniformly distributed in a closed and bounded set of a three-dimensional Euclidean space. Four cases are considered for this set: a union of disjoint disks, a union of disjoint balls, a union of disjoint line segments, and the boundary of a polyhedron. In the first three cases, we provide closed-form expressions of the cumulative distribution function and the density. In the last...

  4. Cell- and nuclear-penetrating anti-dsDNA autoantibodies have multiple arginines in CDR3 of VH and increase cellular level of pERK and Bcl-2 in mesangial cells.

    Science.gov (United States)

    Im, Sae-Ran; Im, Sun-Woo; Chung, Hee-Yong; Pravinsagar, Pavithra; Jang, Young-Ju

    2015-10-01

    Investigation of characteristics of cell- and nuclear-penetrating anti-double stranded (ds)DNA autoantibodies (autoAbs) is important to understand pathogenesis of lupus nephritis, but has not been clearly explored. The present study reports that three anti-dsDNA monoclonal autoAbs, which contain more than two arginine residues in their CDR3s of variable heavy domain (VH), penetrated into living murine mesangial cells and the cell nuclei. However, an anti-dsDNA monoclonal Ab (mAb) having only one arginine in the CDR3-VH did not penetrate cells. To assess the contribution of antigen-binding sites, especially the VH, in cell- and nuclear-penetration, we evaluated the characteristics of recombinant single chain Fv(scFv), VH, and variable light domain (VL) of a penetrating mAb. The scFv and VH domain, containing arginine in CDR3-VH maintained the ability to penetrate cells and the cell nuclei, whereas the VL domain, having no arginine in CDR3, did not penetrate cells. The penetratingm Abs, scFv, and VH activated ERK and increased cellular protein levels of Bcl-2, whereas the non-penetrating Ab and VL did not. The cell survival was decreased by the penetrating mAbs, scFv and VH, not by the non-penetrating mAb and VL. The data indicate that an antigen-binding site is required for cell-penetration and that positively-charged arginine residues in CDR3-VH contribute to the cell- and nuclear-penetrating ability of a subset of anti-dsDNA autoAbs. Furthermore,the nuclear-penetrating anti-dsDNA autoAbs could possibly function as a pathogenic factor for lupus nephritis by up-regulating ERK activation and Bcl-2 production in mesangial cells. The cell- and nuclear-penetrating VH domain may be exploited as a vehicle for the intra cellular delivery of various useful molecules.

  5. Geometrical and P.D.E. Methods in the Treatment of the Theory of Shells: Comparing Euclidean and Affine Approaches

    Directory of Open Access Journals (Sweden)

    Salvador Gigena

    2014-01-01

    Full Text Available The use of differential equations methods in the approach, treatment, and solution of problems in diverse areas of geometry, particularly in affine differential geometry is well known and prolific, where they have proven to be quite fruitful when it comes to the obtainment of definite results. It is perhaps lesser known that the same kind of those very same methods has been and is currently being used to treat developments in some specific areas of applied sciences, such as the theory of shells where, similarly, they can be proven to be quite effective as well. In this paper we precisely show that such is the case in two particular, related instances: the historic approach of the classical, Euclidean part of the theory pursued by Fritz John, in the past century, and the more recent expositions that we ourselves have dedicated to the affine counterpart of the theory.

  6. Weighted Euclidean distance based approach as a multiple attribute decision making method for plant or facility layout design selection

    Directory of Open Access Journals (Sweden)

    R. Venkata Rao

    2012-04-01

    Full Text Available In response to increasing inflexible customer demands and to improve the competitive advantage, industrial organizations have to adopt strategies to achieve cost reduction, continual quality improvement, increased customer service and on-time delivery performance. Selection of the most suitable plant or facility layout design for an organization is one among the most important strategic issues to fulfill all these above-mentioned objectives. Nowadays, many industrial organizations have come to realize the importance of proper selection of the plant or facility layout design to survive in the global competitive market. Selecting the proper layout design from a given set of candidate alternatives is a difficult task, as many potential qualitative and quantitative criteria need to be considered. This paper proposes a Euclidean distance based approach (WEDBA as a multiple attribute decision making method to deal with the complex plant or facility layout design problems of the industrial environment. Three examples are included to illustrate the approach.

  7. Proto-oncogene mRNA levels and activities of multiple transcription factors in C3H 10T 1/2 murine embryonic fibroblasts exposed to 835.62 and 847.74 MHz cellular phone communication frequency radiation.

    Science.gov (United States)

    Goswami, P C; Albee, L D; Parsian, A J; Baty, J D; Moros, E G; Pickard, W F; Roti Roti, J L; Hunt, C R

    1999-03-01

    This study was designed to determine whether two differently modulated radiofrequencies of the type generally used in cellular phone communications could elicit a general stress response in a biological system. The two modulations and frequencies studied were a frequency-modulated continuous wave (FMCW) with a carrier frequency of 835.62 MHz and a code division multiple-access (CDMA) modulation centered on 847.74 MHz. Changes in proto-oncogene expression, determined by measuring Fos, Jun, and Myc mRNA levels as well as by the DNA-binding activity of the AP1, AP2 and NF-kappaB transcription factors, were used as indicators of a general stress response. The effect of radiofrequency exposure on proto-oncogene expression was assessed (1) in exponentially growing C3H 10T 1/2 mouse embryo fibroblasts during their transition to plateau phase and (2) during transition of serum-deprived cells to the proliferation cycle after serum stimulation. Exposure of serum-deprived cells to 835.62 MHz FMCW or 847.74 MHz CDMA microwaves (at an average specific absorption rate, SAR, of 0.6 W/kg) did not significantly change the kinetics of proto-oncogene expression after serum stimulation. Similarly, these exposures did not affect either the Jun and Myc mRNA levels or the DNA-binding activity of AP1, AP2 and NF-kappaB in exponential cells during transit to plateau-phase growth. Therefore, these results suggest that the radiofrequency exposure is unlikely to elicit a general stress response in cells of this cell line under these conditions. However, statistically significant increases (approximately 2-fold, P = 0.001) in Fos mRNA levels were detected in exponential cells in transit to the plateau phase and in plateau-phase cells exposed to 835.62 MHz FMCW microwaves. For 847.74 MHz CDMA exposure, the increase was 1.4-fold (P = 0.04). This increase in Fos expression suggests that expression of specific genes could be affected by radiofrequency exposure. PMID:10073668

  8. Epigenetics and Cellular Metabolism

    Science.gov (United States)

    Xu, Wenyi; Wang, Fengzhong; Yu, Zhongsheng; Xin, Fengjiao

    2016-01-01

    Living eukaryotic systems evolve delicate cellular mechanisms for responding to various environmental signals. Among them, epigenetic machinery (DNA methylation, histone modifications, microRNAs, etc.) is the hub in transducing external stimuli into transcriptional response. Emerging evidence reveals the concept that epigenetic signatures are essential for the proper maintenance of cellular metabolism. On the other hand, the metabolite, a main environmental input, can also influence the processing of epigenetic memory. Here, we summarize the recent research progress in the epigenetic regulation of cellular metabolism and discuss how the dysfunction of epigenetic machineries influences the development of metabolic disorders such as diabetes and obesity; then, we focus on discussing the notion that manipulating metabolites, the fuel of cell metabolism, can function as a strategy for interfering epigenetic machinery and its related disease progression as well. PMID:27695375

  9. Architected Cellular Materials

    Science.gov (United States)

    Schaedler, Tobias A.; Carter, William B.

    2016-07-01

    Additive manufacturing enables fabrication of materials with intricate cellular architecture, whereby progress in 3D printing techniques is increasing the possible configurations of voids and solids ad infinitum. Examples are microlattices with graded porosity and truss structures optimized for specific loading conditions. The cellular architecture determines the mechanical properties and density of these materials and can influence a wide range of other properties, e.g., acoustic, thermal, and biological properties. By combining optimized cellular architectures with high-performance metals and ceramics, several lightweight materials that exhibit strength and stiffness previously unachievable at low densities were recently demonstrated. This review introduces the field of architected materials; summarizes the most common fabrication methods, with an emphasis on additive manufacturing; and discusses recent progress in the development of architected materials. The review also discusses important applications, including lightweight structures, energy absorption, metamaterials, thermal management, and bioscaffolds.

  10. Epigenetics and Cellular Metabolism

    Science.gov (United States)

    Xu, Wenyi; Wang, Fengzhong; Yu, Zhongsheng; Xin, Fengjiao

    2016-01-01

    Living eukaryotic systems evolve delicate cellular mechanisms for responding to various environmental signals. Among them, epigenetic machinery (DNA methylation, histone modifications, microRNAs, etc.) is the hub in transducing external stimuli into transcriptional response. Emerging evidence reveals the concept that epigenetic signatures are essential for the proper maintenance of cellular metabolism. On the other hand, the metabolite, a main environmental input, can also influence the processing of epigenetic memory. Here, we summarize the recent research progress in the epigenetic regulation of cellular metabolism and discuss how the dysfunction of epigenetic machineries influences the development of metabolic disorders such as diabetes and obesity; then, we focus on discussing the notion that manipulating metabolites, the fuel of cell metabolism, can function as a strategy for interfering epigenetic machinery and its related disease progression as well.

  11. Asymptotic behaviour of the first moment of the number of steps in the by-excess and by-deficiency Euclidean algorithms

    International Nuclear Information System (INIS)

    The first moments for the number of steps in different Euclidean algorithms are considered. For these moments asymptotic formulae with new remainder terms are obtained using refined estimates for sums of fractional parts and some ideas in Selberg's elementary proof of the prime number theorem. Bibliography: 12 titles.

  12. Asymptotic behaviour of the first moment of the number of steps in the by-excess and by-deficiency Euclidean algorithms

    Science.gov (United States)

    Frolenkov, Dmitrii A.

    2012-02-01

    The first moments for the number of steps in different Euclidean algorithms are considered. For these moments asymptotic formulae with new remainder terms are obtained using refined estimates for sums of fractional parts and some ideas in Selberg's elementary proof of the prime number theorem. Bibliography: 12 titles.

  13. 三维欧氏距离变换的一种新方法%A New Method of Three-Dimensional Euclidean Distance Transform

    Institute of Scientific and Technical Information of China (English)

    诸葛婴; 田捷; 王蔚洪

    2001-01-01

    常见的三维距离变换算法大都是对城市街区、棋盘等二维近似欧氏距离变换算法的三维扩展,得到的依然是近似欧氏距离.提出一种新的三维欧氏距离变换算法,可以得到完全欧氏距离,时间复杂度为O(n3logn).将该算法应用于三维医学CT图像内部软组织的显示,取得了较好的效果.%Most existing algorithms of three-dimensional distance transformare extensions of two-dimensional approximate Euclidean distance transform algorithms such as the city block/chessboard. Such algorithms can only get the approximate Euclidean distance. A new method of three-dimensional true Euclidean distance transform is presented in this paper. The proposed method can get the true Euclidean distance with time complexity O(n3*log n). Moreover, this method is used to render the soft tissue in three-dimensional medical CT images, and good result has been obtained.

  14. Improved Algorithm of Complete Three-dimensional Euclidean Distance Transform%三维完全欧氏距离变换的改进算法

    Institute of Scientific and Technical Information of China (English)

    董箭; 彭认灿; 郑义东

    2013-01-01

    三维欧氏距离变换是对由黑白像素构成的三维二值图像中所有像素找出其到最近黑色像素的欧氏距离.在对现有三维欧氏距离变换算法进行深入研究的基础上,充分利用二维欧氏距离变换的结果,进一步减少参与距离计算和比较的黑点个数,进而提出了三维完全欧氏距离变换的改进算法.整个算法的时间复杂度为O((n3log n)/ψ (n)).并将该改进算法应用于海底污染源的扩散分析,取得了较好的效果.%The three-dimensional euclidean distance transform converts a three-dimensional image into one where each pixel has a value equal to its euclidean distance to the nearest foreground pixel. After having lucubrated the existent algorithm of three-dimensional euclidean distance transform, the paper makes full use of the result of binary euclidean distance transform to reduce the number of pixels in distance calculation and comparison, and the improved algorithm of complete three-dimensional euclidean distance transform with time complexity 0((n log n)/(p(n))has been put forward. Moreover, the improved algorithm is used to analyse pollution diffusion, and good result has been obtained.

  15. Generalized Noise Clustering Based on Non-Euclidean Distance%基于非欧氏距离的广义噪声聚类

    Institute of Scientific and Technical Information of China (English)

    何光普; 李敏; 武斌; 武小红

    2008-01-01

    Based on a new distance,Dave's generalized noise clustering (GNC) algorithm is extended to non-Euclidean generalized noise clustering (NGNC) model.Different from fuzzy c-means (FCM) model and GNC model which are based on Euclidean distance,the presented model is based on non-Euclidean distance.Based on robust statistical point of view and influence function,the non-Euclidean distance is more robust than the Euclidean distance.So the NGNC algorithm is more robust than the GNC algorithm.Moreover,with the new distance NGNC can deal with noises or outliers better than GNC and fuzzy c-means (FCM).The experimental results show the better performance of NGNC algorithm.%利用一种新的距离测度将Dave的广义噪声聚类(GNC)扩展成非欧氏距离的广义噪声聚类(NGNC).模糊C-均值聚类(FCM)和广义噪声聚类都是基于欧氏距离的模型,与它们不同之处在于NGNC是基于非欧氏距离的模型,建立在鲁棒统计观点和势函数基础上,这种非欧氏距离比欧氏距离更加鲁棒,因此NGNC算法比GNC算法更加鲁棒.并且,建立在新的距离测度上的NGNC在处理噪声和野值方面比GNC和FCM更好.实验结果表明了NGNC的良好特性.

  16. Cellular Response to Irradiation

    Institute of Scientific and Technical Information of China (English)

    LIU Bo; YAN Shi-Wei

    2011-01-01

    To explore the nonlinear activities of the cellular signaling system composed of one transcriptional arm and one protein-interaction arm, we use an irradiation-response module to study the dynamics of stochastic interactions.It is shown that the oscillatory behavior could be described in a unified way when the radiation-derived signal and noise are incorporated.

  17. The New Cellular Immunology

    Science.gov (United States)

    Claman, Henry N.

    1973-01-01

    Discusses the nature of the immune response and traces many of the discoveries that have led to the present state of knowledge in immunology. The new cellular immunology is directing its efforts toward improving health by proper manipulation of the immune mechanisms of the body. (JR)

  18. Cellular Therapy for Heart Failure.

    Science.gov (United States)

    Psaltis, Peter J; Schwarz, Nisha; Toledo-Flores, Deborah; Nicholls, Stephen J

    2016-01-01

    The pathogenesis of cardiomyopathy and heart failure (HF) is underpinned by complex changes at subcellular, cellular and extracellular levels in the ventricular myocardium. For all of the gains that conventional treatments for HF have brought to mortality and morbidity, they do not adequately address the loss of cardiomyocyte numbers in the remodeling ventricle. Originally conceived to address this problem, cellular transplantation for HF has already gone through several stages of evolution over the past two decades. Various cell types and delivery routes have been implemented to positive effect in preclinical models of ischemic and nonischemic cardiomyopathy, with pleiotropic benefits observed in terms of myocardial remodeling, systolic and diastolic performance, perfusion, fibrosis, inflammation, metabolism and electrophysiology. To a large extent, these salubrious effects are now attributed to the indirect, paracrine capacity of transplanted stem cells to facilitate endogenous cardiac repair processes. Promising results have also followed in early phase human studies, although these have been relatively modest and somewhat inconsistent. This review details the preclinical and clinical evidence currently available regarding the use of pluripotent stem cells and adult-derived progenitor cells for cardiomyopathy and HF. It outlines the important lessons that have been learned to this point in time, and balances the promise of this exciting field against the key challenges and questions that still need to be addressed at all levels of research, to ensure that cell therapy realizes its full potential by adding to the armamentarium of HF management. PMID:27280304

  19. Calculus of spatial distribution of absorbed dose to cellular level by Monte Carlo simulation for a radio-labelled peptide with {sup 188}Re and with nuclear internalization : preliminary results; Calculo de la distribucion espacial de dosis absorbida a nivel celular por simulacion Monte Carlo para un peptido radiomarcado con {sup 188}Re y con internalizacion nuclear : resultados preliminares

    Energy Technology Data Exchange (ETDEWEB)

    Rojas C, E. L. [ININ, Carretera Mexico-Toluca s/n, 52750 Ocoyoacac, Estado de Mexico (Mexico); Santos C, C. L. [Universidad Autonoma del Estado de Mexico, Paseo Tollocan y Jesus Carranza, Toluca 50120, Estado de Mexico (Mexico)], e-mail: leticia.rojas@inin.gob.mx

    2009-10-15

    The {sup 188}Re is a radionuclide of radiation gamma emitter, useful in obtaining of gamma-graphic images, but it is also emitter of beta radiations and Auger electrons. A bio-molecule directed to a specific receptor of a cancer cell labeled with a emitter radionuclide of beta particles and Auger electrons, as the {sup 188}Re-Tat-Bombesin, it has the potential to be used in radiotherapy of molecular targets for its capacity to penetrate to cellular nucleus. In this system, the radiation dose is distributed in way located at microscopic levels in sub cellular specific places, where Auger emissions contributes of significant way in absorbed dose. The cellular dosimetry is realized in most of cases, using analytic or semi analytical methods, for example the cellular MIRD methodology. However, it is required to complement these calculations simulating the electrons transport and considering experimental bio kinetics data. Therefore, in this work preliminary results are presented of dosimetric calculation to sub cellular level for {sup 188}Re-Tat-Bombesin by Monte Carlo simulation, using the 2008 version of PENELOPE: PENEASY code. The spatial distribution of absorbed dose in membrane, cytoplasm and nucleus, was calculated with geometry of a cell of 10 {mu}m of diameter, a nucleus of 2 {mu}m of ratio and membrane of 0.2 {mu}m of thickness, considering elementary constitution for each cellular compartment proposal in literature. The total number of disintegrations at sub cellular level was evaluated integrating the activity in function of time starting from experimental bio kinetics data in mamma cancer cells MDA-MB231. The preliminary results show that 46.4% of total disintegrations for unit of captured activity by cell occurs in nucleus, 38.4% in membrane and 15.2% in cytoplasm. The due absorbed dose to Auger electrons for 1 Bq of {sup 188}Re located in cellular membrane were respectively of 1.32E-1 and 1.43E-1 Gy in cytoplasm and nucleus. (Author)

  20. Magnetic Cellular Switches

    OpenAIRE

    Overby, Darryl R.; Alenghat, Francis J.; Montoya-Zavala, Martín; Bei, HuCheng; Oh, Philmo; Karavitis, John; Ingber, Donald E.

    2004-01-01

    This paper focuses on the development of magnetic cellular switches to enable magnetic control of intracellular functions in living mammalian cells, including receptor signal transduction and gene transcription. Our approach takes advantage of the mechanosensitivity of adenosine 3′,5′-monophosphate (cAMP) induction and downstream transcription controlled by the cAMP regulatory element (CRE) to engineer gene constructs that optically report gene expression in living cells. We activate transcri...

  1. Cellular therapy in Tuberculosis

    Directory of Open Access Journals (Sweden)

    Shreemanta K. Parida

    2015-03-01

    Full Text Available Cellular therapy now offer promise of potential adjunct therapeutic options for treatment of drug-resistant tuberculosis (TB. We review here the role of Mesenchymal stromal cells, (MSCs, as well as other immune effector cells in the therapy of infectious diseases with a focus on TB. MSCs represent a population of tissue-resident non-hematopoietic adult progenitor cells which home into injured tissues increase the proliferative potential of broncho-alveolar stem cells and restore lung epithelium. MSCs have been shown to be immune-modulatory and anti-inflammatory mediated via cell-cell contacts as well as soluble factors. We discuss the functional profile of MSCs and their potential use for adjunct cellular therapy of multi-drug resistant TB, with the aim of limiting tissue damage, and to convert unproductive inflammatory responses into effective anti-pathogen directed immune responses. Adjunct cellular therapy could potentially offer salvage therapy options for patients with drug-resistant TB, increase clinically relevant anti-M.tuberculosis directed immune responses and possibly shorten the duration of anti-TB therapy.

  2. A Possible Cosmological Application of Some Thermodynamic Properties of the Black Body Radiation in n-Dimensional Euclidean Spaces

    Directory of Open Access Journals (Sweden)

    Julian Gonzalez-Ayala

    2015-06-01

    Full Text Available In this work, we present the generalization of some thermodynamic properties of the black body radiation (BBR towards an n-dimensional Euclidean space. For this case, the Planck function and the Stefan–Boltzmann law have already been given by Landsberg and de Vos and some adjustments by Menon and Agrawal. However, since then, not much more has been done on this subject, and we believe there are some relevant aspects yet to explore. In addition to the results previously found, we calculate the thermodynamic potentials, the efficiency of the Carnot engine, the law for adiabatic processes and the heat capacity at constant volume. There is a region at which an interesting behavior of the thermodynamic potentials arises: maxima and minima appear for the n—dimensional BBR system at very high temperatures and low dimensionality, suggesting a possible application to cosmology. Finally, we propose that an optimality criterion in a thermodynamic framework could be related to the 3—dimensional nature of the universe.

  3. A possible cosmological application of some thermodynamic properties of the black body radiation in $n-$dimensional Euclidean spaces

    CERN Document Server

    Gonzalez-Ayala, Julian; Cordero, Rubén; Angulo-Brown, F

    2015-01-01

    In this work we present the generalization of some thermodynamic properties of the black body radiation (BBR) towards an $n-$dimensional Euclidean space. For this case the Planck function and the Stefan-Boltzmann law have already been given by Landsberg and de Vos and some adjustments by Menon and Agrawal. However, since then no much more has been done on this subject and we believe there are some relevant aspects yet to explore. In addition to the results previously found we calculate the thermodynamic potentials, the efficiency of the Carnot engine, the law for adiabatic processes and the heat capacity at constant volume. There is a region at which an interesting behavior of the thermodynamic potentials arise, maxima and minima appear for the $n-d$ BBR system at very high temperatures and low dimensionality, suggesting a possible application to cosmology. Finally we propose that an optimality criterion in a thermodynamic framework could have to do with the $3-d$ nature of the universe.

  4. Multi-resolution Shape Analysis via Non-Euclidean Wavelets: Applications to Mesh Segmentation and Surface Alignment Problems.

    Science.gov (United States)

    Kim, Won Hwa; Chung, Moo K; Singh, Vikas

    2013-01-01

    The analysis of 3-D shape meshes is a fundamental problem in computer vision, graphics, and medical imaging. Frequently, the needs of the application require that our analysis take a multi-resolution view of the shape's local and global topology, and that the solution is consistent across multiple scales. Unfortunately, the preferred mathematical construct which offers this behavior in classical image/signal processing, Wavelets, is no longer applicable in this general setting (data with non-uniform topology). In particular, the traditional definition does not allow writing out an expansion for graphs that do not correspond to the uniformly sampled lattice (e.g., images). In this paper, we adapt recent results in harmonic analysis, to derive Non-Euclidean Wavelets based algorithms for a range of shape analysis problems in vision and medical imaging. We show how descriptors derived from the dual domain representation offer native multi-resolution behavior for characterizing local/global topology around vertices. With only minor modifications, the framework yields a method for extracting interest/key points from shapes, a surprisingly simple algorithm for 3-D shape segmentation (competitive with state of the art), and a method for surface alignment (without landmarks). We give an extensive set of comparison results on a large shape segmentation benchmark and derive a uniqueness theorem for the surface alignment problem.

  5. Cellular functions of the microprocessor.

    Science.gov (United States)

    Macias, Sara; Cordiner, Ross A; Cáceres, Javier F

    2013-08-01

    The microprocessor is a complex comprising the RNase III enzyme Drosha and the double-stranded RNA-binding protein DGCR8 (DiGeorge syndrome critical region 8 gene) that catalyses the nuclear step of miRNA (microRNA) biogenesis. DGCR8 recognizes the RNA substrate, whereas Drosha functions as an endonuclease. Recent global analyses of microprocessor and Dicer proteins have suggested novel functions for these components independent of their role in miRNA biogenesis. A HITS-CLIP (high-throughput sequencing of RNA isolated by cross-linking immunoprecipitation) experiment designed to identify novel substrates of the microprocessor revealed that this complex binds and regulates a large variety of cellular RNAs. The microprocessor-mediated cleavage of several classes of RNAs not only regulates transcript levels, but also modulates alternative splicing events, independently of miRNA function. Importantly, DGCR8 can also associate with other nucleases, suggesting the existence of alternative DGCR8 complexes that may regulate the fate of a subset of cellular RNAs. The aim of the present review is to provide an overview of the diverse functional roles of the microprocessor.

  6. Quantum deformations of $D=4$ Euclidean, Lorentz, Kleinian and quaternionic $\\mathfrak{o}^{\\star}(4)$ symmetries in unified $\\mathfrak{o}(4;\\mathbb{C})$ setting

    CERN Document Server

    Borowiec, A; Tolstoy, V N

    2015-01-01

    We employ new calculational technique and present complete list of classical $r$-matrices for $D=4$ complex homogeneous orthogonal Lie algebra $\\mathfrak{o}(4;\\mathbb{C})$, the rotational symmetry of four-dimensional complex space-time. Further applying reality conditions we obtain the classical $r$-matrices for all possible real forms of $\\mathfrak{o}(4;\\mathbb{C})$: Euclidean $\\mathfrak{o}(4)$, Lorentz $\\mathfrak{o}(3,1)$, Kleinian $\\mathfrak{o}(2,2)$ and quaternionic $\\mathfrak{o}^{\\star}(4)$ Lie algebras. For $\\mathfrak{o}(3,1)$ we get known four classical $D=4$ Lorentz $r$-matrices, but for other real Lie algebras (Euclidean, Kleinian, quaternionic) we provide new results.

  7. 欧氏空间中n维Pedoe不等式的推广及应用%Generlization of n-Dimsensional Pedoe Inequalities in Euclidean Space and Application

    Institute of Scientific and Technical Information of China (English)

    杨世国; 王文

    2011-01-01

    The theory and method of distance geometry were used to study the problems of geometric inequalities for the edge-length and volume of two n-dimensional simplexes in the Euclidean space En. Two forms of the improved n-dimensional Peng-Chang inequalities were established. From these we obtained the generalization of n-dimensional Pedoe inequalities in the Euclidean space En.%利用距离几何的理论与方法,研究欧氏空间En中两个n维单形的棱长与体积的几何不等式,建立了n维单形两种加强形式的彭-常不等式,从而推广了En中n维Pedoe不等式.

  8. A note on Padé approximant in extended euclidean algorithm%浅析Padé逼近的扩展欧几里德方法

    Institute of Scientific and Technical Information of China (English)

    李志刚; 陈佘喜

    2012-01-01

    介绍Padé逼近的一般理论,通过引入扩展欧几里德算法给出对任何形式幂级数(n,m)阶Padé逼近的一种计算方法;还给出该方法求Padé逼近的一个应用实例.%The general theory about Pade approximants was introduced, and a new way of computations was provided, that with extended Euclidean algorithm of an (m,n)-Pade approximant to a formal power series. In addiotions, an application example was given by applying Extended Euclidean Algorithm to obtain Pade approximation.

  9. 在细胞水平上对高温珊瑚白化的初步研究%Preliminary study of coral bleaching at cellular level under thermal stress

    Institute of Scientific and Technical Information of China (English)

    李淑; 余克服; 陈天然; 施祺

    2011-01-01

    Global warming and abnormal high temperature cause coral-zooxanthellae symbiosis collapse and significant loss of zooxanthellae (coral bleaching), and further lead to degradation of coral reef ecosystems. In this study, the authors collected six species of corals from the Luhuitou fringing reef in Sanya, and designed a mesocosm experiment that those corals were cultured under a high temperature stress, for investigating the diversity of tolerance among different coral symbiosis at cellular level. The results are as follows. The diversity of tolerance among different coral species under acute thermal stresses was related to their morphologies: branching corals showed the lowest tolerance in thermal stress, while the foliose and massive corals showed stronger tolerance, which was similar to the result in the field monitoring. Different coral species showed different ways of zooxanthellae loss under thermal stresses: zooxanthellae discharging continuously, like Pocillopora damicornis; zooxanthellae discharging partly and followed by coral tissues containing zooxanthellae separating from skeletons,like Acropora hyacinthus and Acropora brueggemanni; the residual zooxauthellae in coral tissues using mitotic proliferation to replenish their numbers quickly, like Pavona decussate; cell necrosis of zooxanthellae in coral tissues, like Porites lutea. This study confirmed that the role of coral host and zooxanthellae should be considered simultaneously in further studies of coral responses to global warming.%全球变暖背景下的异常高温能够导致珊瑚及其虫黄藻组成的共生体系崩溃,虫黄藻大量损失,出现珊瑚白化,并可能进一步导致珊瑚礁生态系统退化.文章通过对6种造礁石珊瑚的急性高温胁迫实验,分析不同种属的石珊瑚虫黄藻共生体系对高温的耐受性差异,为全球变暖背景下珊瑚群落演替趋势提供理论依据.结果显示:1)在急性高温胁迫下,石珊瑚耐受的差异性与其

  10. 欧氏空间En中Pedoe不等式推广%Generalizations of n-dimensional Pedoe inequality in euclidean space En

    Institute of Scientific and Technical Information of China (English)

    王文; 杨世国

    2012-01-01

    Using the theory and method of distance geometry, some geometric inequalities for n-dimensional simplexes were proved in Euclidean space En, from which the n-dimensional Pedoe inequality and Peng-Chang's inequality involving the edge-lengths of two simplexes were generalized. In addition, the k-n type Pedoe inequality and Peng-Chang's inequality in Euclidean space were generalized. Finally, a new inequality for an n-dimensional simplex in Euclidean space was obtained.%应用距离几何的理论与方法,先证明了欧氏空间中n维单形的几个几何不等式,然后建立了加强形式的涉及两个单形棱长的n维Pedoe不等式和彭-常不等式以及k-n型Pedoe不等式和彭-常不等式.另外还建立了一个重要不等式.

  11. The ACADS gene variation spectrum in 114 patients with short-chain acyl-CoA dehydrogenase (SCAD) deficiency is dominated by missense variations leading to protein misfolding at the cellular level

    DEFF Research Database (Denmark)

    Pedersen, Christina Bak; Kølvrå, Steen; Kølvraa, Agnete;

    2008-01-01

    , 26 missense, one start codon, and two stop codon variations. In vitro import studies of variant SCAD proteins in isolated mitochondria from SCAD deficient (SCAD-/-) mice demonstrated an increased tendency of the abnormal proteins to misfold and aggregate compared to the wild-type, a phenomenon that...... often leads to gain-of-function cellular phenotypes. However, no correlation was found between the clinical phenotype and the degree of SCAD dysfunction. We propose that SCAD deficiency should be considered as a disorder of protein folding that can lead to clinical disease in combination with other...

  12. Multiuser Cellular Network

    CERN Document Server

    Bao, Yi; Chen, Ming

    2011-01-01

    Modern radio communication is faced with a problem about how to distribute restricted frequency to users in a certain space. Since our task is to minimize the number of repeaters, a natural idea is enlarging coverage area. However, coverage has restrictions. First, service area has to be divided economically as repeater's coverage is limited. In this paper, our fundamental method is to adopt seamless cellular network division. Second, underlying physics content in frequency distribution problem is interference between two close frequencies. Consequently, we choose a proper frequency width of 0.1MHz and a relevantly reliable setting to apply one frequency several times. We make a few general assumptions to simplify real situation. For instance, immobile users yield to homogenous distribution; repeaters can receive and transmit information in any given frequency in duplex operation; coverage is mainly decided by antenna height. Two models are built up to solve 1000 users and 10000 users situations respectively....

  13. Parametric study of double cellular detonation structure

    Science.gov (United States)

    Khasainov, B.; Virot, F.; Presles, H.-N.; Desbordes, D.

    2013-05-01

    A parametric numerical study is performed of a detonation cellular structure in a model gaseous explosive mixture whose decomposition occurs in two successive exothermic reaction steps with markedly different characteristic times. Kinetic and energetic parameters of both reactions are varied in a wide range in the case of one-dimensional steady and two-dimensional (2D) quasi-steady self-supported detonations. The range of governing parameters of both exothermic steps is defined where a "marked" double cellular structure exists. It is shown that the two-level cellular structure is completely governed by the kinetic parameters and the local overdrive ratio of the detonation front propagating inside large cells. Furthermore, since it is quite cumbersome to use detailed chemical kinetics in unsteady 2D case, the proposed work should help to identify the mixtures and the domain of their equivalence ratio where double detonation structure could be observed.

  14. Never-ageing cellular senescence

    OpenAIRE

    Ogrunc, Müge; d’Adda di Fagagna, Fabrizio

    2011-01-01

    Cellular senescence was historically discovered as a form of cellular ageing of in vitro cultured cells. It has been under the spotlight following the evidence of oncogene-induced senescence in vivo and its role as a potent tumour suppressor mechanism. Presently, a PubMed search using keywords ‘cellular senescence and cancer’ reveals 8398 number of references (by April 2011) showing that while our knowledge of senescence keeps expanding, the complexity of the phenomenon keeps us – researchers...

  15. The State of Cellular Probes

    OpenAIRE

    Yim, Youngbin

    2003-01-01

    Cellular probe technology is one of several potentially promising technologies for obtaining accurate travel time information. In 1996, the Federal Communications Commission (FCC) mandated E911 requirements that cellular location be provided when 911 emergency calls come in to emergency management authorities. The E911 requirements allow 50 -300 meters from the emergency call location, depending on the type of cellular phone technology used and whether handset-based or network-based solutions...

  16. Euclidean gravity attracts

    OpenAIRE

    Bakker MR; Smit, J.

    1993-01-01

    We look at gravitational attraction in simplicial gravity using the dynamical triangulation method. On the dynamical triangulation configurations we measure quenched propagators of a free massive scalar field. The masses measured from these propagators show that gravitational attraction is present.

  17. Euclidean gravity attracts

    CERN Document Server

    De Bakker, B V; Bakker, Bas de; Smit, Jan

    1994-01-01

    We look at gravitational attraction in simplicial gravity using the dynamical triangulation method. On the dynamical triangulation configurations we measure quenched propagators of a free massive scalar field. The masses measured from these propagators show that gravitational attraction is present.

  18. Euclidean Geometry via Programming.

    Science.gov (United States)

    Filimonov, Rossen; Kreith, Kurt

    1992-01-01

    Describes the Plane Geometry System computer software developed at the Educational Computer Systems laboratory in Sofia, Bulgaria. The system enables students to use the concept of "algorithm" to correspond to the process of "deductive proof" in the development of plane geometry. Provides an example of the software's capability and compares it to…

  19. Intensity Thresholds on Raw Acceleration Data: Euclidean Norm Minus One (ENMO) and Mean Amplitude Deviation (MAD) Approaches

    Science.gov (United States)

    Bakrania, Kishan; Yates, Thomas; Rowlands, Alex V.; Esliger, Dale W.; Bunnewell, Sarah; Sanders, James; Davies, Melanie; Khunti, Kamlesh; Edwardson, Charlotte L.

    2016-01-01

    Objectives (1) To develop and internally-validate Euclidean Norm Minus One (ENMO) and Mean Amplitude Deviation (MAD) thresholds for separating sedentary behaviours from common light-intensity physical activities using raw acceleration data collected from both hip- and wrist-worn tri-axial accelerometers; and (2) to compare and evaluate the performances between the ENMO and MAD metrics. Methods Thirty-three adults [mean age (standard deviation (SD)) = 27.4 (5.9) years; mean BMI (SD) = 23.9 (3.7) kg/m2; 20 females (60.6%)] wore four accelerometers; an ActiGraph GT3X+ and a GENEActiv on the right hip; and an ActiGraph GT3X+ and a GENEActiv on the non-dominant wrist. Under laboratory-conditions, participants performed 16 different activities (11 sedentary behaviours and 5 light-intensity physical activities) for 5 minutes each. ENMO and MAD were computed from the raw acceleration data, and logistic regression and receiver-operating-characteristic (ROC) analyses were implemented to derive thresholds for activity discrimination. Areas under ROC curves (AUROC) were calculated to summarise performances and thresholds were assessed via executing leave-one-out-cross-validations. Results For both hip and wrist monitor placements, in comparison to the ActiGraph GT3X+ monitors, the ENMO and MAD values derived from the GENEActiv devices were observed to be slightly higher, particularly for the lower-intensity activities. Monitor-specific hip and wrist ENMO and MAD thresholds showed excellent ability for separating sedentary behaviours from motion-based light-intensity physical activities (in general, AUROCs >0.95), with validation indicating robustness. However, poor classification was experienced when attempting to isolate standing still from sedentary behaviours (in general, AUROCs <0.65). The ENMO and MAD metrics tended to perform similarly across activities and accelerometer brands. Conclusions Researchers can utilise these robust monitor-specific hip and wrist ENMO and MAD

  20. Vanderbilt University Study Creates New Roadmap for Cellular Activity - Office of Cancer Clinical Proteomics Research

    Science.gov (United States)

    Scientists studying cellular processes have long sought to measure redox modifications because they provide one of the normal layers of cell control. But redox disruption or oxidative stress at the cellular level can also create a pathway to diseases like

  1. About Strongly Universal Cellular Automata

    Directory of Open Access Journals (Sweden)

    Maurice Margenstern

    2013-09-01

    Full Text Available In this paper, we construct a strongly universal cellular automaton on the line with 11 states and the standard neighbourhood. We embed this construction into several tilings of the hyperbolic plane and of the hyperbolic 3D space giving rise to strongly universal cellular automata with 10 states.

  2. Cellular Dynamic Simulator: An Event Driven Molecular Simulation Environment for Cellular Physiology

    Science.gov (United States)

    Byrne, Michael J.; Waxham, M. Neal; Kubota, Yoshihisa

    2010-01-01

    In this paper, we present the Cellular Dynamic Simulator (CDS) for simulating diffusion and chemical reactions within crowded molecular environments. CDS is based on a novel event driven algorithm specifically designed for precise calculation of the timing of collisions, reactions and other events for each individual molecule in the environment. Generic mesh based compartments allow the creation / importation of very simple or detailed cellular structures that exist in a 3D environment. Multiple levels of compartments and static obstacles can be used to create a dense environment to mimic cellular boundaries and the intracellular space. The CDS algorithm takes into account volume exclusion and molecular crowding that may impact signaling cascades in small sub-cellular compartments such as dendritic spines. With the CDS, we can simulate simple enzyme reactions; aggregation, channel transport, as well as highly complicated chemical reaction networks of both freely diffusing and membrane bound multi-protein complexes. Components of the CDS are generally defined such that the simulator can be applied to a wide range of environments in terms of scale and level of detail. Through an initialization GUI, a simple simulation environment can be created and populated within minutes yet is powerful enough to design complex 3D cellular architecture. The initialization tool allows visual confirmation of the environment construction prior to execution by the simulator. This paper describes the CDS algorithm, design implementation, and provides an overview of the types of features available and the utility of those features are highlighted in demonstrations. PMID:20361275

  3. Mapping of cellular iron using hyperspectral fluorescence imaging in a cellular model of Parkinson's disease

    Science.gov (United States)

    Oh, Eung Seok; Heo, Chaejeong; Kim, Ji Seon; Lee, Young Hee; Kim, Jong Min

    2013-05-01

    Parkinson's disease (PD) is characterized by progressive dopaminergic cell loss in the substantianigra (SN) and elevated iron levels demonstrated by autopsy and with 7-Tesla magnetic resonance imaging. Direct visualization of iron with live imaging techniques has not yet been successful. The aim of this study is to visualize and quantify the distribution of cellular iron using an intrinsic iron hyperspectral fluorescence signal. The 1-methyl-4-phenylpyridinium (MPP+)-induced cellular model of PD was established in SHSY5Y cells. The cells were exposed to iron by treatment with ferric ammonium citrate (FAC, 100 μM) for up to 6 hours. The hyperspectral fluorescence imaging signal of iron was examined usinga high- resolution dark-field optical microscope system with signal absorption for the visible/ near infrared (VNIR) spectral range. The 6-hour group showed heavy cellular iron deposition compared with the small amount of iron accumulation in the 1-hour group. The cellular iron was dispersed in a small, particulate form, whereas extracellular iron was detected in an aggregated form. In addition, iron particles were found to be concentrated on the cell membrane/edge of shrunken cells. The cellular iron accumulation readily occurred in MPP+-induced cells, which is consistent with previous studies demonstrating elevated iron levels in the SN in PD. This direct iron imaging methodology could be applied to analyze the physiological role of iron in PD, and its application might be expanded to various neurological disorders involving other metals, such as copper, manganese or zinc.

  4. MIMO Communication for Cellular Networks

    CERN Document Server

    Huang, Howard; Venkatesan, Sivarama

    2012-01-01

    As the theoretical foundations of multiple-antenna techniques evolve and as these multiple-input multiple-output (MIMO) techniques become essential for providing high data rates in wireless systems, there is a growing need to understand the performance limits of MIMO in practical networks. To address this need, MIMO Communication for Cellular Networks presents a systematic description of MIMO technology classes and a framework for MIMO system design that takes into account the essential physical-layer features of practical cellular networks. In contrast to works that focus on the theoretical performance of abstract MIMO channels, MIMO Communication for Cellular Networks emphasizes the practical performance of realistic MIMO systems. A unified set of system simulation results highlights relative performance gains of different MIMO techniques and provides insights into how best to use multiple antennas in cellular networks under various conditions. MIMO Communication for Cellular Networks describes single-user,...

  5. Influence of income on tertiary students acquisition of cellular products

    Directory of Open Access Journals (Sweden)

    G. A.P Drotsky

    2007-12-01

    Full Text Available Purpose: The purpose of the article is to determine whether there are any differences between high and low-income group students in their selection of a cellular phone brand or network operator. Design/Methodology/Approach: Four hypotheses are set to determine if there are any significant differences between the two income groups in current decision-making. It is established that there exist no significant difference between high and low-income students in their selection of cellular phones and network operators. The levels of agreement or disagreement on various statements do, however, give an indication of the importance that students place on aspects that they view as important when acquiring a cellular phone or network operator.Findings: In the article, it is established that no significant differences exist between the two income groups. The levels of agreement or disagreement indicate the importance that subscription method, social value, service quality and branding has on student decision-making. Implications: The article provides a better understanding of the influence that income plays in student's decision-making in acquiring cellular products and services. Possible future research in student cellular usage can be guided through the information obtained in this article. Originality/Value: The article provides information to cellular network operators, service providers and cellular phone manufactures regarding the influence of income on students' acquisition of cellular products and services. Information from the article can assist in the establishment of marketing plans for the student market by these role players.

  6. Cellular systems biology profiling applied to cellular models of disease.

    Science.gov (United States)

    Giuliano, Kenneth A; Premkumar, Daniel R; Strock, Christopher J; Johnston, Patricia; Taylor, Lansing

    2009-11-01

    Building cellular models of disease based on the approach of Cellular Systems Biology (CSB) has the potential to improve the process of creating drugs as part of the continuum from early drug discovery through drug development and clinical trials and diagnostics. This paper focuses on the application of CSB to early drug discovery. We discuss the integration of protein-protein interaction biosensors with other multiplexed, functional biomarkers as an example in using CSB to optimize the identification of quality lead series compounds.

  7. Actual problems of cellular cardiomyoplasty

    Directory of Open Access Journals (Sweden)

    Bulat Kaupov

    2010-04-01

    Full Text Available The paper provides review of cellular technologies used incardiology, describes types of cellular preparations depending onsources of cells and types of compounding cells. The generalmechanisms of therapies with stem cells applications are described.Use of cellular preparations for treatment of cardiovascular diseasesand is improvement of the forecast at patients with heartinsufficiency of various genesis is considered as alternative topractice with organ transplantations. Efforts of biotechnologicallaboratories are directed on search of optimum population of cellsfor application in cardiology and studying of mechanisms andfactors regulating function of cardiac stem cells.

  8. Validation of origins of tea samples using partial least squares analysis and Euclidean distance method with near-infrared spectroscopy data

    Science.gov (United States)

    He, Wei; Zhou, Jian; Cheng, Hao; Wang, Liyuan; Wei, Kang; Wang, Weifeng; Li, Xinghui

    2012-02-01

    In today's global food markets, the ability to trace the origins of agricultural products is becoming increasingly important. We developed an efficient procedure for validating the authenticity and origin of tea samples where Partial Least Squares and Euclidean Distance methods, based on near-infrared spectroscopy data, were combined to classify tea samples from different tea producing areas. Four models for identification of authenticity of tea samples were constructed and utilized in our two-step procedure. High accuracy rates of 98.60%, 97.90%, 97.55%, and 99.83% for the calibration set, and 97.19%, 97.54%, 97.83%, 100% for test set, were achieved. After the first identification step, employing the four origin authenticity models, followed by the second step using the Euclidean Distance method, accuracy rates for specific origin identification were 98.43% in the calibration set and 96.84% in the test set. This method, employing two-step analysis of multi-origin model, accurately identified the origin of tea samples collected in four different areas. This study provided a potential reference method for the detection of "geographical indication" of agricultural products' and is available for use in traceability of origin studies.

  9. 基于核的图像欧氏距离人脸识别%Image Euclidean distance based on kernel for face recognition

    Institute of Scientific and Technical Information of China (English)

    郝建东; 张伟伟

    2011-01-01

    引进了两幅图像之间的一种新的距离度量方法——图像欧氏距离,该距离是利用核函数对传统的欧氏距离进行改进而得到的.在此基础上,设计了一种新的分类识别方法——基于核的图像欧氏距离人脸识别方法,并应用于人脸识别中.为验证该算法的可行性,对人脸图像进行DCT变换得到预处理样本,并在ORL和Yale人脸库上进行多角度的比较实验.分析实验结果表明,该方法优于其它距离分类器算法.%A new distance named Euclidean distance in the image is proposed, which consists of the idea of kernel function. On this basis, a novel recognition method called image Euclidean distance based on kernel for face recognition is designed. In order to verify the feasibility of the proposed method, firstly, it takes pretreatment to facial images to get pretreatment samples. The experiments show that this new algorithm outperforms other distance classifier algorithms.

  10. The Inverse Problem on Property of Quadratic Function in n-Dimensional Euclidean Space%关于Rn空间上二次函数的反问题

    Institute of Scientific and Technical Information of China (English)

    邓勇

    2015-01-01

    The author studied an important property of quadratic function on the tangent plane in n-dimensional Euclidean space. This result published on journal of Kashgar Teachers College in 2012,33(6),and its title was“The property on Tangent Planes of a Quadratic Funcion in Rn”.In this paper,we further its inverse problem, that it is proved that a scalar-valued function f(x) defined in n-dimensional Euclidean space must be quadratic,if the intersection of tangent planes x1 and x2 always contains the midpoint of the line joining x1 and x2 .%在研究了Rn空间上二次函数切平面的一个重要性的基础上,进一步研究其反问题,即证明了定义于Rn空间上的任意一个纯量函数f(x),如果它在点x1和x2处切平面的交线始终包含连接点x1和x2线段的中点,那么f(x)必为二次函数。

  11. Weighted Improved Euclidean Localization Algorithm Based on ZigBee%基于ZigBee的加权改进Euclidean定位算法

    Institute of Scientific and Technical Information of China (English)

    金纯; 何山; 胡建农; 周亮; 徐洪刚

    2011-01-01

    RSSI-based triangle and centroid location sometimes can not work, in order to solve this problem, weighted improved Euclidean localization algorithm is proposed based on ZigBee. The algorithm uses the least square method to derive the parameters of the environment, and then the weighted improved Euclidean localization algorithm is used to locate the unknown nodes. The result of the simulation and experiment proves that it can be applied to some applications.%针对基于RSSI的三角形质心定位算法会出现不能工作的情况,提出了基于ZigBee的加权改进Euclidean定位算法.此算法利用最小二乘法得到环境参数,然后利用加权改进Euclidean定位算法对未知节点进行定位.仿真和实验表明,该算法具有一定的实用价值.

  12. 基于优化欧氏距离的协同过滤推荐%Collaborative Filtering Recommendation Based on Optimization Euclidean Distance

    Institute of Scientific and Technical Information of China (English)

    陈小辉; 高燕

    2015-01-01

    由于推荐系统中用户对项目的评价数据具有多样性和稀疏性的特点,传统的相似性度量算法不能有效查找相似邻居,本文提出一种基于优化欧氏距离的邻居相似度计算方法,在欧氏距离计算的基础上引入归一化处理和杰卡德相似系数,并最终作出评价预测和推荐。在典型数据集上的实验结果显示该算法能够有效提高协同过滤推荐系统的推荐性能。%User evaluation data of items often are of the biodiversity and sparse characteristic in collaborative filtering recommen-dation system, the traditional similarity measurement algorithm cannot effectively find similar neighbors, this paper proposed a neighbor similarity computing algorithm based on optimized Euclidean distance. The algorithm introduced normalization and Jac-card similarity coefficient based on Euclidean distance calculation, and finally made the evaluation prediction and recommenda-tion. The experiments result on typical dataset show that the algorithm can effectively improve the performance of collaborative fil-tering recommendation system.

  13. IL-2 expression in activated human memory FOXP3+ cells critically depends on the cellular levels of FOXP3 as well as of four transcription factors of T cell activation

    Directory of Open Access Journals (Sweden)

    Hanna eBendfeldt

    2012-08-01

    Full Text Available The human CD4+FOXP3+ T cell population is heterogeneous and consists of various subpopulations which remain poorly defined. Anergy and suppression are two main functional characteristics of FOXP3+Treg cells. We used the anergic behavior of FOXP3+Treg cells for a better discrimination and characterization of such subpopulations. We compared IL-2-expressing with IL-2-nonexpressing cells within the memory FOXP3+ T cell population. In contrast to IL-2 nonexpressing FOXP3+ cells, IL-2-expressing FOXP3+ cells exhibit intermediate characteristics of Treg and Th cells concerning the Treg cell markers CD25, GITR, and Helios. Besides lower levels of FOXP3, they also have higher levels of the transcription factors NFATc2, c-Fos, NF-Bp65, and c-Jun. An approach combining flow cytometric measurements with statistical interpretation for quantitative transcription factor analysis suggest that the physiological expression levels not only of FOXP3 but also of NFATc2, c-Jun, c-Fos, and NF-Bp65 are limiting for the decision whether IL-2 is expressed or not in activated peripheral human memory FOXP3+ cells. These findings demonstrate that concomitant high levels of NFATc2, c-Jun, c-Fos, and NF-Bp65 lead in addition to potential IL-2 expression in those FOXP3+ cells with low levels of FOXP3. We hypothesize that not only the level of FOXP3 expression but also the amounts of the four transcription factors studied represent determining factors for the anergic phenotype of FOXP3+ Treg cells.

  14. Cytokines as cellular communicators

    Directory of Open Access Journals (Sweden)

    R. Debets

    1996-01-01

    Full Text Available Cytokines and their receptors are involved in the pathophysiology of many diseases. Here we present a detailed review on cytokines, receptors and signalling routes, and show that one important lesson from cytokine biology is the complex and diverse regulation of cytokine activity. The activity of cytokines is controlled at the level of transcription, translation, storage, processing, posttranslational modification, trapping, binding by soluble proteins, and receptor number and/or function. Translation of this diverse regulation in strategies aimed at the control of cytokine activity will result in the development of more specific and selective drugs to treat diseases.

  15. Cellular vs. organ approaches to dose estimates

    International Nuclear Information System (INIS)

    The cellular distribution of tissue-incorporated radionuclides has generally been neglected in the dosimetry of internal emitters. Traditional dosimetry assumes homogeneous distribution of radionuclides in organs of interest, while presuming that the ranges of particulate radiations are large relative to typical cell diameters. The macroscopic distribution of dose thus calculated has generally served as a sufficient approximation for the energy deposited within radiosensitive sites. However, with the increasing utilization of intracellular agents, such as thallium-201, it has become necessary to examine the microscopic distribution of energy at the cellular level. This is particularly important in the instance of radionuclides that decay by electron capture or by internal conversion with the release of Auger and Coster-Kronig electrons. In many instances, these electrons are released as a dense shower of low-energy particles with ranges of subcellular dimensions. The high electron density in the immediate vicinity of the decaying atom produces a focal deposition of energy that far exceeds the average dose taken over several cell diameters. These studies point out the increasing need to take into account the microscopic distribution of dose on the cellular level as radionuclides distributed in cells become more commonplace, especially if the decay involves electron capture or internal conversion. As radiotracers are developed for the measurement of intracellular functions these factors should be given greater consideration. 16 references, 5 figures, 5 tables

  16. Pirin inhibits cellular senescence in melanocytic cells.

    Science.gov (United States)

    Licciulli, Silvia; Luise, Chiara; Scafetta, Gaia; Capra, Maria; Giardina, Giuseppina; Nuciforo, Paolo; Bosari, Silvano; Viale, Giuseppe; Mazzarol, Giovanni; Tonelli, Chiara; Lanfrancone, Luisa; Alcalay, Myriam

    2011-05-01

    Cellular senescence has been widely recognized as a tumor suppressing mechanism that acts as a barrier to cancer development after oncogenic stimuli. A prominent in vivo model of the senescence barrier is represented by nevi, which are composed of melanocytes that, after an initial phase of proliferation induced by activated oncogenes (most commonly BRAF), are blocked in a state of cellular senescence. Transformation to melanoma occurs when genes involved in controlling senescence are mutated or silenced and cells reacquire the capacity to proliferate. Pirin (PIR) is a highly conserved nuclear protein that likely functions as a transcriptional regulator whose expression levels are altered in different types of tumors. We analyzed the expression pattern of PIR in adult human tissues and found that it is expressed in melanocytes and has a complex pattern of regulation in nevi and melanoma: it is rarely detected in mature nevi, but is expressed at high levels in a subset of melanomas. Loss of function and overexpression experiments in normal and transformed melanocytic cells revealed that PIR is involved in the negative control of cellular senescence and that its expression is necessary to overcome the senescence barrier. Our results suggest that PIR may have a relevant role in melanoma progression. PMID:21514450

  17. Cellular mechanisms during vascular development

    OpenAIRE

    Blum, Yannick

    2012-01-01

    The vascular system is an essential organ in vertebrate animals and provides the organism with enough oxygen and nutrients. It is composed of an interconnected network of blood vessels, which form using a number of different morphogenetic mechanisms. Angiogenesis describes the formation of new blood vessels from preexisting vessels. A number of molecular pathways have been shown to be essential during angiogenesis. However, cellular architecture of blood vessels as well as cellular mechanisms...

  18. Cellular automaton for chimera states

    OpenAIRE

    García-Morales, Vladimir

    2016-01-01

    A minimalistic model for chimera states is presented. The model is a cellular automaton (CA) which depends on only one adjustable parameter, the range of the nonlocal coupling, and is built from elementary cellular automata and the majority (voting) rule. This suggests the universality of chimera-like behavior from a new point of view: Already simple CA rules based on the majority rule exhibit this behavior. After a short transient, we find chimera states for arbitrary initial conditions, the...

  19. Mathematical Modeling of Cellular Metabolism.

    Science.gov (United States)

    Berndt, Nikolaus; Holzhütter, Hermann-Georg

    2016-01-01

    Cellular metabolism basically consists of the conversion of chemical compounds taken up from the extracellular environment into energy (conserved in energy-rich bonds of organic phosphates) and a wide array of organic molecules serving as catalysts (enzymes), information carriers (nucleic acids), and building blocks for cellular structures such as membranes or ribosomes. Metabolic modeling aims at the construction of mathematical representations of the cellular metabolism that can be used to calculate the concentration of cellular molecules and the rates of their mutual chemical interconversion in response to varying external conditions as, for example, hormonal stimuli or supply of essential nutrients. Based on such calculations, it is possible to quantify complex cellular functions as cellular growth, detoxification of drugs and xenobiotic compounds or synthesis of exported molecules. Depending on the specific questions to metabolism addressed, the methodological expertise of the researcher, and available experimental information, different conceptual frameworks have been established, allowing the usage of computational methods to condense experimental information from various layers of organization into (self-) consistent models. Here, we briefly outline the main conceptual frameworks that are currently exploited in metabolism research.

  20. Cellular heterogeneity and live cell arrays.

    Science.gov (United States)

    Walling, Maureen A; Shepard, Jason R E

    2011-07-01

    In the past decade, the tendency to move from a global, one-size-fits-all treatment philosophy to personalized medicine is based, in part, on the nuanced differences and sub-classifications of disease states. Our knowledge of these varied states stems from not only the ability to diagnose, classify, and perform experiments on cell populations as a whole, but also from new technologies that allow interrogation of cell populations at the individual cell level. Such departures from conventional thinking are driven by the recognition that clonal cell populations have numerous activities that manifest as significant levels of non-genetic heterogeneity. Clonal populations by definition originate from a single genetic origin so are regarded as having a high level of homogeneity as compared to genetically distinct cell populations. However, analysis at the single cell level has revealed a different phenomenon; cells and organisms require an inherent level of non-genetic heterogeneity to function properly, and in some cases, to survive. The growing understanding of this occurrence has lead to the development of methods to monitor, analyze, and better characterize the heterogeneity in cell populations. Following the trend of DNA- and protein microarrays, platforms capable of simultaneously monitoring each cell in a population have been developed. These cellular microarray platforms and other related formats allow for continuous monitoring of single live cells and simultaneously generate individual cell and average population data that are more descriptive and information-rich than traditional bulk methods. These technological advances have helped develop a better understanding of the intricacies associated with biological processes and afforded greater insight into complex biological systems. The associated instruments, techniques, and reagents now allow for highly multiplexed analyses, which enable multiple cellular activities, processes, or pathways to be monitored

  1. Hierarchical Cellular Structures in High-Capacity Cellular Communication Systems

    CERN Document Server

    Jain, R K; Agrawal, N K

    2011-01-01

    In the prevailing cellular environment, it is important to provide the resources for the fluctuating traffic demand exactly in the place and at the time where and when they are needed. In this paper, we explored the ability of hierarchical cellular structures with inter layer reuse to increase the capacity of mobile communication network by applying total frequency hopping (T-FH) and adaptive frequency allocation (AFA) as a strategy to reuse the macro and micro cell resources without frequency planning in indoor pico cells [11]. The practical aspects for designing macro- micro cellular overlays in the existing big urban areas are also explained [4]. Femto cells are inducted in macro / micro / pico cells hierarchical structure to achieve the required QoS cost effectively.

  2. Continuum representations of cellular solids

    Energy Technology Data Exchange (ETDEWEB)

    Neilsen, M.K.

    1993-09-01

    Cellular materials consist of interconnected struts or plates which form cells. The struts or plates are constructed from a variety of metals, polymers, ceramics and wood products. Cellular materials are often used in impact limiters for shipping containers to protect the contents from accidental impact events. These materials exhibit a variety of complex behavior when subjected to crushing loads. This research focuses on the development of continuum representations of cellular solids that can be used in the finite element analysis of shipping container accidents. A significant portion of this work is the development of a new methodology to relate localized deformations to appropriate constitutive descriptions. This methodology provides the insight needed to select constitutive descriptions for cellular solids that capture the localized deformations that are observed experimentally. Constitutive relations are developed for two different cellular materials, aluminum honeycomb and polyurethane foam. These constitutive relations are based on plasticity and continuum damage theories. Plasticity is used to describe the permanent deformation exhibited by both aluminum honeycomb and polyurethane foam. Continuum damage is needed to capture the change in elastic parameters due to cracking of the polyurethane cell wall materials. The new constitutive description of polyurethane foam is implemented in both static and dynamic finite element codes, and analytical and numerical predictions are compared with available experimental data.

  3. Prognosis of Different Cellular Generations

    Directory of Open Access Journals (Sweden)

    Preetish Ranjan

    2013-04-01

    Full Text Available Technological advancement in mobile telephony from 1G to 3G, 4G and 5G has a very axiomatic fact that made an entire world a global village. The cellular system employs a different design approach and technology that most commercial radio and television system use. In the cellular system, the service area is divided into cells and a transmitter is designed to serve an individual cell. The system seeks to make efficient use of available channels by using low-power transmitters to allow frequency reuse at a smaller distance. Maximizing the number of times each channel can be reused in a given geographical area is the key to an efficient cellular system design. During the past three decades, the world has seen significant changes in telecommunications industry. There have been some remarkable aspects to the rapid growth in wireless communications, as seen by the large expansion in mobile systems. This paper focuses on “Past, Present & Future of Cellular Telephony” and some light has been thrown upon the technologies of the cellular systems, namely 1G, 2G, 2.5G, 3G and future generations like 4G and 5G systems as well.

  4. The Role of Lipids in Cellular Architecture and Function

    OpenAIRE

    Lopes Sampaio, Julio

    2011-01-01

    All cells are delimited by membranes that protect the cell from the surrounding environment. In eukaryotic cells the same principle applies at subcellular level where membranes delimit functional cell organelles. The membrane structure, properties and function are defined in part by their lipid composition. Lipidomics is the large‐scale study of pathways and networks of cellular lipids in biological systems. It involves the identification and quantitation of cellular lipid molecular species a...

  5. Aging, cellular senescence, and cancer.

    Science.gov (United States)

    Campisi, Judith

    2013-01-01

    For most species, aging promotes a host of degenerative pathologies that are characterized by debilitating losses of tissue or cellular function. However, especially among vertebrates, aging also promotes hyperplastic pathologies, the most deadly of which is cancer. In contrast to the loss of function that characterizes degenerating cells and tissues, malignant (cancerous) cells must acquire new (albeit aberrant) functions that allow them to develop into a lethal tumor. This review discusses the idea that, despite seemingly opposite characteristics, the degenerative and hyperplastic pathologies of aging are at least partly linked by a common biological phenomenon: a cellular stress response known as cellular senescence. The senescence response is widely recognized as a potent tumor suppressive mechanism. However, recent evidence strengthens the idea that it also drives both degenerative and hyperplastic pathologies, most likely by promoting chronic inflammation. Thus, the senescence response may be the result of antagonistically pleiotropic gene action. PMID:23140366

  6. 平面Bonnesen型不等式的几点注记%NOTES ON BONNESEN-STYLE INEQUALITY IN THE EUCLIDEAN PLANE

    Institute of Scientific and Technical Information of China (English)

    何刚; 徐文学

    2012-01-01

    In this paper, we first investigate the Bonnesen-style inequality for compact domain in the Euclidean plane. By using the perimeters and areas of the compact domain and its convex hull, we obtain some new Bonnesen-style inequalities and two new Bonnesen-style inequalities for planar compact convex domain expressing by its inradius and outradius.%本文研究了平面紧域的Bonnesen型不等式.利用紧域及其凸包的周长和面积得到一些新的Bonnesen型不等式以及两个用最大内切圆半径与最小外接圆半径表示的Bonnesen型不等式.

  7. Empirical multiscale networks of cellular regulation.

    Directory of Open Access Journals (Sweden)

    Benjamin de Bivort

    2007-10-01

    Full Text Available Grouping genes by similarity of expression across multiple cellular conditions enables the identification of cellular modules. The known functions of genes enable the characterization of the aggregate biological functions of these modules. In this paper, we use a high-throughput approach to identify the effective mutual regulatory interactions between modules composed of mouse genes from the Alliance for Cell Signaling (AfCS murine B-lymphocyte database which tracks the response of approximately 15,000 genes following chemokine perturbation. This analysis reveals principles of cellular organization that we discuss along four conceptual axes. (1 Regulatory implications: the derived collection of influences between any two modules quantifies intuitive as well as unexpected regulatory interactions. (2 Behavior across scales: trends across global networks of varying resolution (composed of various numbers of modules reveal principles of assembly of high-level behaviors from smaller components. (3 Temporal behavior: tracking the mutual module influences over different time intervals provides features of regulation dynamics such as duration, persistence, and periodicity. (4 Gene Ontology correspondence: the association of modules to known biological roles of individual genes describes the organization of functions within coexpressed modules of various sizes. We present key specific results in each of these four areas, as well as derive general principles of cellular organization. At the coarsest scale, the entire transcriptional network contains five divisions: two divisions devoted to ATP production/biosynthesis and DNA replication that activate all other divisions, an "extracellular interaction" division that represses all other divisions, and two divisions (proliferation/differentiation and membrane infrastructure that activate and repress other divisions in specific ways consistent with cell cycle control.

  8. Adaptive stochastic cellular automata: Applications

    Science.gov (United States)

    Qian, S.; Lee, Y. C.; Jones, R. D.; Barnes, C. W.; Flake, G. W.; O'Rourke, M. K.; Lee, K.; Chen, H. H.; Sun, G. Z.; Zhang, Y. Q.; Chen, D.; Giles, C. L.

    1990-09-01

    The stochastic learning cellular automata model has been applied to the problem of controlling unstable systems. Two example unstable systems studied are controlled by an adaptive stochastic cellular automata algorithm with an adaptive critic. The reinforcement learning algorithm and the architecture of the stochastic CA controller are presented. Learning to balance a single pole is discussed in detail. Balancing an inverted double pendulum highlights the power of the stochastic CA approach. The stochastic CA model is compared to conventional adaptive control and artificial neural network approaches.

  9. Cellular automaton for chimera states

    Science.gov (United States)

    García-Morales, Vladimir

    2016-04-01

    A minimalistic model for chimera states is presented. The model is a cellular automaton (CA) which depends on only one adjustable parameter, the range of the nonlocal coupling, and is built from elementary cellular automata and the majority (voting) rule. This suggests the universality of chimera-like behavior from a new point of view: Already simple CA rules based on the majority rule exhibit this behavior. After a short transient, we find chimera states for arbitrary initial conditions, the system spontaneously splitting into stable domains separated by static boundaries, some synchronously oscillating and the others incoherent. When the coupling range is local, nontrivial coherent structures with different periodicities are formed.

  10. Cellular senescence in aging primates.

    Science.gov (United States)

    Herbig, Utz; Ferreira, Mark; Condel, Laura; Carey, Dee; Sedivy, John M

    2006-03-01

    The aging of organisms is characterized by a gradual functional decline of all organ systems. Mammalian somatic cells in culture display a limited proliferative life span, at the end of which they undergo an irreversible cell cycle arrest known as replicative senescence. Whether cellular senescence contributes to organismal aging has been controversial. We investigated telomere dysfunction, a recently discovered biomarker of cellular senescence, and found that the number of senescent fibroblasts increases exponentially in the skin of aging baboons, reaching >15% of all cells in very old individuals. In addition, the same cells contain activated ataxia-telangiectasia mutated kinase and heterochromatinized nuclei, confirming their senescent status. PMID:16456035

  11. Effect of cellular mobility on immune response

    Science.gov (United States)

    Pandey, R. B.; Mannion, R.; Ruskin, H. J.

    2000-08-01

    Mobility of cell types in our HIV immune response model is subject to an intrinsic mobility and an explicit directed mobility, which is governed by Pmob. We investigate how restricting the explicit mobility, while maintaining the innate mobility of a viral-infected cell, affects the model's results. We find that increasing the explicit mobility of the immune system cells leads to viral dominance for certain levels of viral mutation. We conclude that increasing immune system cellular mobility indirectly increases the virus’ inherent mobility.

  12. Analysis of cellular manufacturing systems

    NARCIS (Netherlands)

    Heragu, Sunderesh; Meng, Gang; Zijm, Henk; Ommeren, van Jan-Kees

    2001-01-01

    In this paper, we present an open queuing network modeling approach to estimate performance measures of a cellular manufacturing layout. It is assumed a layout and production data for a planning period of specified length are available. The production data takes into account, processing and handli

  13. Cellular uptake of metallated cobalamins

    DEFF Research Database (Denmark)

    Tran, MQT; Stürup, Stefan; Lambert, Ian H.;

    2016-01-01

    Cellular uptake of vitamin B12-cisplatin conjugates was estimated via detection of their metal constituents (Co, Pt, and Re) by inductively coupled plasma mass spectrometry (ICP-MS). Vitamin B12 (cyano-cob(iii)alamin) and aquo-cob(iii)alamin [Cbl-OH2](+), which differ in the β-axial ligands (CN(-...

  14. Activation of the NLRP3 inflammasome by cellular labile iron.

    Science.gov (United States)

    Nakamura, Kyohei; Kawakami, Toru; Yamamoto, Naoki; Tomizawa, Miyu; Fujiwara, Tohru; Ishii, Tomonori; Harigae, Hideo; Ogasawara, Kouetsu

    2016-02-01

    Cellular labile iron, which contains chelatable redox-active Fe(2+), has been implicated in iron-mediated cellular toxicity leading to multiple organ dysfunction. Iron homeostasis is controlled by monocytes/macrophages through their iron recycling and storage capacities. Furthermore, iron sequestration by monocytes/macrophages is regulated by pro-inflammatory cytokines including interleukin-1, highlighting the importance of these cells in the crosstalk between inflammation and iron homeostasis. However, a role for cellular labile iron in monocyte/macrophage-mediated inflammatory responses has not been defined. Here we describe how cellular labile iron activates the NLRP3 inflammasome in human monocytes. Stimulation of lipopolysaccharide-primed peripheral blood mononuclear cells with ferric ammonium citrate increases the level of cellular Fe(2+) levels in monocytes and induces production of interleukin-1β in a dose-dependent manner. This ferric ammonium citrate-induced interleukin-1β production is dependent on caspase-1 and is significantly inhibited by an Fe(2+)-specific chelator. Ferric ammonium citrate consistently induced interleukin-1β secretion in THP1 cells, but not in NLRP3-deficient THP1 cells, indicating a requirement for the NLRP3 inflammasome. Additionally, activation of the inflammasome is mediated by potassium efflux, reactive oxygen species-mediated mitochondrial dysfunction, and lysosomal membrane permeabilization. Thus, these results suggest that monocytes/macrophages not only sequestrate iron during inflammation, but also mediate inflammation in response to cellular labile iron, which provides novel insights into the role of iron in chronic inflammation. PMID:26577567

  15. Cellular Phone Base Stations: Technology and Exposures (invited paper)

    International Nuclear Information System (INIS)

    The principles and practice of cellular radio systems for mobile communications are presented using GSM 1800 as a reference system. In particular, the concepts of small cells and frequency re-use and the components of radio base station technology are described. Public and cellular broadcasting have been widely available for many years and a brief history is given to indicate length of exposure from these sources. National and international guidelines for safe exposure to non-ionising radiation are used by cellular operators to define exclusion zones around transmitting antennas. A methodology for calculating an exclusion zone is described, together with an example for a typical antenna configuration. Estimated levels of exposure near practical base stations are given and comparisons made with other sources of RF radiation. Finally, the digital nature of today's cellular radio systems, such as GSM, are explained and the implications described. (author)

  16. Molecular and cellular level of action of digitalis.

    Science.gov (United States)

    Charlemagne, D

    1993-04-01

    The pharmacological receptor of cardiac glycosides is the Na+/K(+)-ATPase which consists of a catalytic alpha (M(r) = 112,000) and glycosylated beta (M(r) = 35,000) subunit. The enzyme is responsible for the vectorial transport across the sarcolemma of three Na+ ions outward and two K+ ions inward against their electrochemical gradient. Specific inhibition of the Na+ pump by digitalis induces a positive inotropic effect by increasing the intracellular Na+ concentration which in turn induces an increase in the intracellular Ca2+ concentration by the Na+/Ca2+ exchange and an increase in the Ca2+ pool of the sarcoplasmic reticulum; toxic effects are observed at higher doses of cardiac glycosides leading to spontaneous calcium release from the sarcoplasmic reticulum. Three isoforms of the alpha catalytic subunit have been identified by molecular cloning. They share a high homology in the deduced amino acid sequence with eight transmembrane domains. The ouabain binding domain is located on the extracellular side and ouabain sensitivity depends mainly on the two residues at the border of the first extracellular domain. The isoforms differed by their ouabain sensitivity, are expressed in a tissue-specific and hormonally-regulated manner. Moreover, expression of the isoforms and their ouabain sensitivity vary from species to species with an alpha 1 isoform of very low affinity being the major isoform (80%) in the adult rat heart and an alpha 1 isoform of high affinity representing 50% of total alpha mRNA abundance in the human heart. Therefore the effect of digitalis on the heart depends mainly on the isoform which is expressed and on the regulation of their expression according to age, hormonal influence and pathology. PMID:7684015

  17. Hypoxic sensitizers: radiobiological studies at the cellular level

    International Nuclear Information System (INIS)

    The nitroimidazoles have been found to selectively sensitize hypoxic cells to the effects of irradiation. The latest in this family of drugs is RO-07-0582, which is able to mimic 80 percent of the oxygen effect at a concentration of 5 mM by modifying the sensitivity of hypoxic cells to single doses of gamma rays; however, it is not a substitution for oxygen in promoting the repair of sublethal radiation damage between split doses. Studies show that it is a powerful cytotoxic agent as well and selectively operates against hypoxic cells. (auth)

  18. Bio-optofluidics and biophotonics at the cellular level

    DEFF Research Database (Denmark)

    Bañas, Andrew Rafael; Palima, Darwin; Tauro, Sandeep;

    2012-01-01

    We present ongoing research and development activities for constructing a compact next generation BioPhotonics Workstation and a Bio-optofluidic Cell Sorter (cell-BOCS) for all-optical micro-manipulation platforms utilizing low numerical aperture beam geometries. Unlike conventional high NA optical...

  19. Cellular solidification of transparent monotectics

    Science.gov (United States)

    Kaulker, W. F.

    1986-01-01

    Understanding how liquid phase particles are engulfed or pushed during freezing of a monotectic is addressed. The additional complication is that the solid-liquid interface is nonplanar due to constitutional undercooling. Some evidence of particle pushing where the particles are the liquid phase of the montectic was already observed. Cellular freezing of the succinonitrile-glycerol system also occurred. Only a few compositions were tested at that time. The starting materials were not especially pure so that cellular interface observed was likely due to the presence of unkown impurities, the major portion of which was water. Topics addressed include: the effort of modeling the particle pushing process using the computer, establishing an apparatus for the determination of phase diagrams, and the measurement of the temperature gradients with a specimen which will solidify on the temperature gradient microscope stage.

  20. Reversibly assembled cellular composite materials.

    Science.gov (United States)

    Cheung, Kenneth C; Gershenfeld, Neil

    2013-09-13

    We introduce composite materials made by reversibly assembling a three-dimensional lattice of mass-produced carbon fiber-reinforced polymer composite parts with integrated mechanical interlocking connections. The resulting cellular composite materials can respond as an elastic solid with an extremely large measured modulus for an ultralight material (12.3 megapascals at a density of 7.2 milligrams per cubic centimeter). These materials offer a hierarchical decomposition in modeling, with bulk properties that can be predicted from component measurements and deformation modes that can be determined by the placement of part types. Because site locations are locally constrained, structures can be produced in a relative assembly process that merges desirable features of fiber composites, cellular materials, and additive manufacturing.

  1. WWW Business Applications Based on the Cellular Model

    Institute of Scientific and Technical Information of China (English)

    Toshio Kodama; Tosiyasu L. Kunii; Yoichi Seki

    2008-01-01

    A cellular model based on the Incrementally Modular Abstraction Hierarchy (IMAH) is a novel model that can represent the architecture of and changes in cyberworlds, preserving invariants from a general level to a specific one. We have developed a data processing system called the Cellular Data System (CDS). In the development of business applications, you can prevent combinatorial explosion in the process of business design and testing by using CDS. In this paper, we have first designed and implemented wide-use algebra on the presentation level. Next, we have developed and verified the effectiveness of two general business applications using CDS: 1) a customer information management system, and 2) an estimate system.

  2. Analysis of cellular manufacturing systems

    OpenAIRE

    Heragu, Sunderesh; Meng, Gang; Zijm, Henk; Ommeren, van, J.C.

    2001-01-01

    In this paper, we present an open queuing network modeling approach to estimate performance measures of a cellular manufacturing layout. It is assumed a layout and production data for a planning period of specified length are available. The production data takes into account, processing and handling set-up times as well as transfer and process batch size information of multiple products that flow through the system. It is assumed that two sets of discrete material handling devices are used fo...

  3. Identification of Nonstationary Cellular Automata

    Institute of Scientific and Technical Information of China (English)

    AndrewI.Adamatzky

    1992-01-01

    The principal feature of nonstationary cellular automata(NCA) is that a local transitiol rule of each cell is changed at each time step depending on neighborhood configuration at previous time step.The identification problem for NCA is extraction of local transition rules and the establishment of mechanism for changing these rules using sequence of NCA configurations.We present serial and parallel algorithms for identification of NCA.

  4. The Origins of Cellular Life

    OpenAIRE

    Schrum, Jason P.; Zhu, Ting F.; SZOSTAK, JACK W.

    2010-01-01

    Understanding the origin of cellular life on Earth requires the discovery of plausible pathways for the transition from complex prebiotic chemistry to simple biology, defined as the emergence of chemical assemblies capable of Darwinian evolution. We have proposed that a simple primitive cell, or protocell, would consist of two key components: a protocell membrane that defines a spatially localized compartment, and an informational polymer that allows for the replication and inheritance of fun...

  5. Stochastic Nature in Cellular Processes

    Institute of Scientific and Technical Information of China (English)

    刘波; 刘圣君; 王祺; 晏世伟; 耿轶钊; SAKATA Fumihiko; GAO Xing-Fa

    2011-01-01

    The importance of stochasticity in cellular processes is increasingly recognized in both theoretical and experimental studies. General features of stochasticity in gene regulation and expression are briefly reviewed in this article, which include the main experimental phenomena, classification, quantization and regulation of noises. The correlation and transmission of noise in cascade networks are analyzed further and the stochastic simulation methods that can capture effects of intrinsic and extrinsic noise are described.

  6. CELLULAR FETAL MICROCHIMERISM IN PREECLAMPSIA

    OpenAIRE

    Gammill, Hilary S; Aydelotte, Tessa M.; Guthrie, Katherine A.; Nkwopara, Evangelyn C.; Nelson, J. Lee

    2013-01-01

    Previous studies have shown elevated concentrations of free fetal deoxyribonucleic acid and erythroblasts in maternal circulation in preeclampsia compared with normal pregnancy. Pluripotent and immunocompetent fetal cells also transfer to the maternal circulation during pregnancy, but whether concentrations of fetal mononuclear cells also differed in preeclampsia was unknown. We sought to quantify cellular fetal microchimerism in maternal circulation in women with preeclampsia and healthy con...

  7. Cellular reactions to patterned biointerfaces

    OpenAIRE

    Schulte, Vera Antonie

    2012-01-01

    The subject of this thesis is to study cellular reactions to topographically, mechanically and biochemically tunable polymeric biomaterials. Different aspects of in vitro cell-biomaterial interactions were systematically studied with the murine fibroblast cell line NIH L929 and primary human dermal fibroblasts (HDFs). Besides a general cytocompatibility assessment of the applied materials and the quantification of cell adhesion per se, cell morphological changes (e.g. cell spreading) and intr...

  8. CELLULAR INTERACTIONS MEDIATED BY GLYCONECTIDS

    OpenAIRE

    Popescu, O.; Sumanovski, L. T.; I. Checiu; Elisabeta Popescu; G. N. Misevic

    1999-01-01

    Cellular interactions involve many types of cell surface molecules and operate via homophilic and/or heterophilic protein-protein and protein-carbohydrate binding. Our investigations in different model-systems (marine invertebrates and mammals) have provided direct evidence that a novel class of primordial proteoglycans, named by us gliconectins, can mediate cell adhesion via a new alternative molecular mechanism of polyvalent carbohydrate-carbohydrate binding. Biochemical characterization of...

  9. Progress of cellular dedifferentiation research

    Institute of Scientific and Technical Information of China (English)

    LIU Hu-xian; HU Da-hai; JIA Chi-yu; FU Xiao-bing

    2006-01-01

    Differentiation, the stepwise specialization of cells, and transdifferentiation, the apparent switching of one cell type into another, capture much of the stem cell spotlight. But dedifferentiation, the developmental reversal of a cell before it reinvents itself, is an important process too. In multicellular organisms, cellular dedifferentiation is the major process underlying totipotency, regeneration and formation of new stem cell lineages. In humans,dedifferentiation is often associated with carcinogenesis.The study of cellular dedifferentiation in animals,particularly early events related to cell fate-switch and determination, is limited by the lack of a suitable,convenient experimental system. The classic example of dedifferentiation is limb and tail regeneration in urodele amphibians, such as salamanders. Recently, several investigators have shown that certain mammalian cell types can be induced to dedifferentiate to progenitor cells when stimulated with the appropriate signals or materials. These discoveries open the possibility that researchers might enhance the endogenous regenerative capacity of mammals by inducing cellular dedifferentiation in vivo.

  10. The insect cellular immune response

    Institute of Scientific and Technical Information of China (English)

    Michael R. Strand

    2008-01-01

    The innate immune system of insects is divided into humoral defenses that include the production of soluble effector molecules and cellular defenses like phagocytosis and encapsulation that are mediated by hemocytes. This review summarizes current understanding of the cellular immune response. Insects produce several terminally differentiated types of hemocytes that are distinguished by morphology, molecular and antigenic markers, and function. The differentiated hemocytes that circulate in larval or nymphal stage insects arise from two sources: progenitor cells produced during embryogenesis and mesodermally derived hematopoietic organs. Regulation of hematopoiesis and hemocyte differentiation also involves several different signaling pathways. Phagocytosis and encapsulation require that hemocytes first recognize a given target as foreign followed by activation of downstream signaling and effector responses. A number of humoral and cellular receptors have been identified that recognize different microbes and multicellular parasites. In turn, activation of these receptors stimulates a number of signaling pathways that regulate different hemocyte functions. Recent studies also identify hemocytes as important sources of a number of humoral effector molecules required for killing different foreign invaders.

  11. Cellular communications a comprehensive and practical guide

    CERN Document Server

    Tripathi, Nishith

    2014-01-01

    Even as newer cellular technologies and standards emerge, many of the fundamental principles and the components of the cellular network remain the same. Presenting a simple yet comprehensive view of cellular communications technologies, Cellular Communications provides an end-to-end perspective of cellular operations, ranging from physical layer details to call set-up and from the radio network to the core network. This self-contained source forpractitioners and students represents a comprehensive survey of the fundamentals of cellular communications and the landscape of commercially deployed

  12. From Cellular Mechanotransduction to Biologically Inspired Engineering

    Science.gov (United States)

    Ingber, Donald E.

    2010-01-01

    This article is based on a lecture I presented as the recipient of the 2009 Pritzker Distinguished Lecturer Award at the Biomedical Engineering Society annual meeting in October 2009. Here, I review more than thirty years of research from my laboratory, beginning with studies designed to test the theory that cells use tensegrity (tensional integrity) architecture to stabilize their shape and sense mechanical signals, which I believed to be critical for control of cell function and tissue development. Although I was trained as a cell biologist, I found that the tools I had at my disposal were insufficient to experimentally test these theories, and thus I ventured into engineering to find critical solutions. This path has been extremely fruitful as it has led to confirmation of the critical role that physical forces play in developmental control, as well as how cells sense and respond to mechanical signals at the molecular level through a process known as cellular mechanotransduction. Many of the predictions of the cellular tensegrity model relating to cell mechanical behaviors have been shown to be valid, and this vision of cell structure led to discovery of the central role that transmembrane adhesion receptors, such as integrins, and the cytoskeleton play in mechanosensing and mechanochemical conversion. In addition, these fundamental studies have led to significant unexpected technology fallout, including development of micromagnetic actuators for non-invasive control of cellular signaling, microfluidic systems as therapeutic extracorporeal devices for sepsis therapy, and new DNA-based nanobiotechnology approaches that permit construction of artificial tensegrities that mimic properties of living materials for applications in tissue engineering and regenerative medicine. PMID:20140519

  13. Euclidean Closed Linear Transformations of Complex Spacetime and generally of Complex Spaces of dimension four endowed with the Same or Different Metric

    Science.gov (United States)

    Vossos, Spyridon; Vossos, Elias

    2016-08-01

    Relativity Theory and the corresponding Relativistic Quantum Mechanics are the fundamental theories of physics. Special Relativity (SR) relates the frames of Relativistic Inertial observers (RIOs), through Linear Spacetime Transformation (LSTT) of linear spacetime. Classic Special Relativity uses real spacetime endowed with Lorentz metric and the frames of two RIOs with parallel spatial axes are always related through Lorentz Boost (LB). This cancels the transitive attribute in parallelism, when three RIOs are related, because LB is not closed transformation, causing Thomas Rotation. In this presentation, we consider closed LSTT of Complex Spacetime, so there is no necessity for spatial axes rotation and all the frames are chosen having parallel spatial axes. The solution is expressed by a 4x4 matrix (Λ) containing components of the complex velocity of one Observer wrt another and two functions depended by the metric of Spacetime. Demanding isometric transformation, it emerges a class of metrics that are in accordance with the closed LSTT and the transformation matrix contains one parameter ω depended by the metric of Spacetime. In case that we relate RIOs with steady metric, it emerges one steady number (ωI ) depended by the metric of Spacetime of the specific SR. If ωI is an imaginary number, the elements of the Λ are complex numbers, so the corresponding spacetime is necessarily complex and there exists real Universal Speed (UI). The specific value ωI =±i gives Vossos transformation (VT) endowed with Lorentz metric (for gii=1) of complex spacetime and invariant spacetime interval (or equivalently invariant speed of light in vacuum), which produce the theory of Euclidean Complex Relativistic Mechanics (ECRMs). If ωI is a real number (ωI #0) the elements of the Λ are real numbers, so the corresponding spacetime is real, but there exist imaginary UI. The specific value ωI =0 gives Galileo Transformation (GT) with the invariant time, in which any other

  14. Modeling In Vitro Cellular Responses to Silver Nanoparticles

    Directory of Open Access Journals (Sweden)

    Dwaipayan Mukherjee

    2014-01-01

    Full Text Available Engineered nanoparticles (NPs have been widely demonstrated to induce toxic effects to various cell types. In vitro cell exposure systems have high potential for reliable, high throughput screening of nanoparticle toxicity, allowing focusing on particular pathways while excluding unwanted effects due to other cells or tissue dosimetry. The work presented here involves a detailed biologically based computational model of cellular interactions with NPs; it utilizes measurements performed in human cell culture systems in vitro, to develop a mechanistic mathematical model that can support analysis and prediction of in vivo effects of NPs. The model considers basic cellular mechanisms including proliferation, apoptosis, and production of cytokines in response to NPs. This new model is implemented for macrophages and parameterized using in vitro measurements of changes in cellular viability and mRNA levels of cytokines: TNF, IL-1b, IL-6, IL-8, and IL-10. The model includes in vitro cellular dosimetry due to nanoparticle transport and transformation. Furthermore, the model developed here optimizes the essential cellular parameters based on in vitro measurements, and provides a “stepping stone” for the development of more advanced in vivo models that will incorporate additional cellular and NP interactions.

  15. Huntington's disease: implications of associated cellular radiosensitivity

    International Nuclear Information System (INIS)

    Ionizing radiation sensitivity was studied in a series of Huntington's Disease (HD) patients and controls by measurement of radiation-induced chromosome aberrations in lymphocytes and by clonogenic survival of lymphoblastoid cell lines. As a group, HD patients were found to be significantly more radioisensitive than controls (p<0.001), but there was an overlap between values for the two groups such that an absolute distinction is not possible. These data are consistent with an association between HD and radiosensitivity but not with identity between HD and a radiosensitive phenotype, so that cellular radiosensitivity cannot be used for individual diagnosis. Analysis of three families including 5 HD patients and 11 first-degree relatives confirmed this conclusion and demonstrated that even within a given family presymptomatic diagnosis cannot be based on measurement of radiosensitivity. However, the common association of cellular radiosensitivity with HD probands and their families provides a potential lead to the identification of HD gene(s) and so to an eventual understanding of the aetiopathogenesis of this disease at the molecular level. (author)

  16. Impaired nonspecific cellular immunity in experimental cholestasis.

    Science.gov (United States)

    Roughneen, P T; Drath, D B; Kulkarni, A D; Rowlands, B J

    1987-11-01

    The abilities of polymorphonuclear leukocytes (PMN) and pulmonary alveolar macrophages (PAM), to demonstrate chemotaxis, phagocytosis, and superoxide release after bile duct ligation in the rat were investigated to determine the effect of cholestasis on nonspecific cellular immune mechanisms. Chemotactic response to C5a and FMLP, phagocytosis of 14C labeled Staphylococcus aureus, and zymosan-induced superoxide release were evaluated 21 days after bile duct ligation (BDL), sham operation, or in normal controls. Serum total bilirubin level was elevated after BDL (p less than 0.01). Chemotactic ability was similar to each group. PMN phagocytic uptake of 14C labeled Staphylococcus aureus was depressed in BDL (p less than 0.05). BDL rats exhibited impaired PAM phagocytic indices and improved PMN superoxide release (p less than 0.03). PAM superoxide release was similar in each study group. Alterations in phagocytic function with cholestasis are important deficits in nonspecific cellular immunity that may contribute to the high incidence of infective complications associated with obstructive jaundice. PMID:2823730

  17. Cellular Auxin Homeostasis:Gatekeeping Is Housekeeping

    Institute of Scientific and Technical Information of China (English)

    Michel Ruiz Rosquete; Elke Barbez; Jürgen Kleine-Vehn

    2012-01-01

    The phytohormone auxin is essential for plant development and contributes to nearly every aspect of the plant life cycle.The spatio-temporal distribution of auxin depends on a complex interplay between auxin metabolism and cell-to-cell auxin transport.Auxin metabolism and transport are both crucial for plant development;however,it largely remains to be seen how these processes are integrated to ensure defined cellular auxin levels or even gradients within tissues or organs.In this review,we provide a glance at very diverse topics of auxin biology,such as biosynthesis,conjugation,oxidation,and transport of auxin.This broad,but certainly superficial,overview highlights the mutual importance of auxin metabolism and transport.Moreover,it allows pinpointing how auxin metabolism and transport get integrated to jointly regulate cellular auxin homeostasis.Even though these processes have been so far only separately studied,we assume that the phytohormonal crosstalk integrates and coordinates auxin metabolism and transport.Besides the integrative power of the global hormone signaling,we additionally introduce the hypothetical concept considering auxin transport components as gatekeepers for auxin responses.

  18. Cellular host responses to gliomas.

    Directory of Open Access Journals (Sweden)

    Joseph Najbauer

    Full Text Available BACKGROUND: Glioblastoma multiforme (GBM is the most aggressive type of malignant primary brain tumors in adults. Molecular and genetic analysis has advanced our understanding of glioma biology, however mapping the cellular composition of the tumor microenvironment is crucial for understanding the pathology of this dreaded brain cancer. In this study we identified major cell populations attracted by glioma using orthotopic rodent models of human glioma xenografts. Marker-specific, anatomical and morphological analyses revealed a robust influx of host cells into the main tumor bed and tumor satellites. METHODOLOGY/PRINCIPAL FINDINGS: Human glioma cell lines and glioma spheroid orthotopic implants were used in rodents. In both models, the xenografts recruited large numbers of host nestin-expressing cells, which formed a 'network' with glioma. The host nestin-expressing cells appeared to originate in the subventricular zone ipsilateral to the tumor, and were clearly distinguishable from pericytes that expressed smooth muscle actin. These distinct cell populations established close physical contact in a 'pair-wise' manner and migrated together to the deeper layers of tumor satellites and gave rise to tumor vasculature. The GBM biopsy xenografts displayed two different phenotypes: (a low-generation tumors (first in vivo passage in rats were highly invasive and non-angiogenic, and host nestin-positive cells that infiltrated into these tumors displayed astrocytic or elongated bipolar morphology; (b high-generation xenografts (fifth passage had pronounced cellularity, were angiogenic with 'glomerulus-like' microvascular proliferations that contained host nestin-positive cells. Stromal cell-derived factor-1 and its receptor CXCR4 were highly expressed in and around glioma xenografts, suggesting their role in glioma progression and invasion. CONCLUSIONS/SIGNIFICANCE: Our data demonstrate a robust migration of nestin-expressing host cells to glioma, which

  19. Estimation in Cellular Radio Systems

    OpenAIRE

    Blom, Jonas; Gunnarsson, Fredrik; Gustafsson, Fredrik

    1999-01-01

    The problem to track time-varying parameters in cellular radio systems is studied, and the focus is on estimation based only on the signals that are readily available. Previous work have demonstrated very good performance, but were relying on analog measurement that are not available. Most of the information is lost due to quantization and sampling at a rate that might be as low as 2 Hz (GSM case). For that matter a maximum likelihood estimator have been designed and exemplified in the case o...

  20. Cellular immune responses to HIV

    Science.gov (United States)

    McMichael, Andrew J.; Rowland-Jones, Sarah L.

    2001-04-01

    The cellular immune response to the human immunodeficiency virus, mediated by T lymphocytes, seems strong but fails to control the infection completely. In most virus infections, T cells either eliminate the virus or suppress it indefinitely as a harmless, persisting infection. But the human immunodeficiency virus undermines this control by infecting key immune cells, thereby impairing the response of both the infected CD4+ T cells and the uninfected CD8+ T cells. The failure of the latter to function efficiently facilitates the escape of virus from immune control and the collapse of the whole immune system.