WorldWideScience

Sample records for cellular defense system

  1. Cellular Defense System Gene Expression Profiling of Human Whole Blood: Opportunities to Predict Health Benefits in Response to Diet12

    OpenAIRE

    Drew, Janice E.

    2012-01-01

    Diet is a critical factor in the maintenance of human cellular defense systems, immunity, inflammation, redox regulation, metabolism, and DNA repair that ensure optimal health and reduce disease risk. Assessment of dietary modulation of cellular defense systems in humans has been limited due to difficulties in accessing target tissues. Notably, peripheral blood gene expression profiles associated with nonhematologic disease are detectable. Coupled with recent innovations in gene expression te...

  2. Pb-induced cellular defense system in the root meristematic cells of Allium sativum L

    Directory of Open Access Journals (Sweden)

    Liu Donghua

    2010-03-01

    Full Text Available Abstract Background Electron microscopy (EM techniques enable identification of the main accumulations of lead (Pb in cells and cellular organelles and observations of changes in cell ultrastructure. Although there is extensive literature relating to studies on the influence of heavy metals on plants, Pb tolerance strategies of plants have not yet been fully explained. Allium sativum L. is a potential plant for absorption and accumulation of heavy metals. In previous investigations the effects of different concentrations (10-5 to 10-3 M of Pb were investigated in A. sativum, indicating a significant inhibitory effect on shoot and root growth at 10-3 to 10-4 M Pb. In the present study, we used EM and cytochemistry to investigate ultrastructural alterations, identify the synthesis and distribution of cysteine-rich proteins induced by Pb and explain the possible mechanisms of the Pb-induced cellular defense system in A. sativum. Results After 1 h of Pb treatment, dictyosomes were accompanied by numerous vesicles within cytoplasm. The endoplasm reticulum (ER with swollen cisternae was arranged along the cell wall after 2 h. Some flattened cisternae were broken up into small closed vesicles and the nuclear envelope was generally more dilated after 4 h. During 24-36 h, phenomena appeared such as high vacuolization of cytoplasm and electron-dense granules in cell walls, vacuoles, cytoplasm and mitochondrial membranes. Other changes included mitochondrial swelling and loss of cristae, and vacuolization of ER and dictyosomes during 48-72 h. In the Pb-treatment groups, silver grains were observed in cell walls and in cytoplasm, suggesting the Gomori-Swift reaction can indirectly evaluate the Pb effects on plant cells. Conclusions Cell walls can immobilize some Pb ions. Cysteine-rich proteins in cell walls were confirmed by the Gomori-Swift reaction. The morphological alterations in plasma membrane, dictyosomes and ER reflect the features of detoxification

  3. Low levels of graphene and graphene oxide inhibit cellular xenobiotic defense system mediated by efflux transporters.

    Science.gov (United States)

    Liu, Su; Jiang, Wei; Wu, Bing; Yu, Jing; Yu, Haiyan; Zhang, Xu-Xiang; Torres-Duarte, Cristina; Cherr, Gary N

    2016-06-01

    Low levels of graphene and graphene oxide (GO) are considered to be environmentally safe. In this study, we analyzed the potential effects of graphene and GO at relatively low concentrations on cellular xenobiotic defense system mediated by efflux transporters. The results showed that graphene (<0.5 μg/mL) and GO (<20 μg/mL) did not decrease cell viability, generate reactive oxygen species, or disrupt mitochondrial function. However, graphene and GO at the nontoxic concentrations could increase calcein-AM (CAM, an indicator of membrane ATP-binding cassette (ABC) transporter) activity) accumulation, indicating inhibition of ABC transporters' efflux capabilities. This inhibition was observed even at 0.005 μg/mL graphene and 0.05 μg/mL GO, which are 100 times and 400 times lower than their lowest toxic concentration from cytotoxicity experiments, respectively. The inhibition of ABC transporters significantly increased the toxicity of paraquat and arsenic, known substrates of ABC transporters. The inhibition of ABC transporters was found to be based on graphene and GO damaging the plasma membrane structure and fluidity, thus altering functions of transmembrane ABC transporters. This study demonstrates that low levels of graphene and GO are not environmentally safe since they can significantly make cell more susceptible to other xenobiotics, and this chemosensitizing activity should be considered in the risk assessment of graphene and GO. PMID:26554512

  4. [Cellular defense system of some synanthropic dipterans inhabitant of bacterially aggressive environment].

    Science.gov (United States)

    Kind, T V

    2014-01-01

    nodulation. A differing case is Syrphidae whe-e charcoal injection produce depletion of hemolymph both from particles and all types of hemocytes. So the specimen of different higher Diptera families can use different schemes of cellular defense reaction. PMID:25509155

  5. Systemic defense signaling in tomato

    Institute of Scientific and Technical Information of China (English)

    LI Changbao; SUN Jiaqiang; JIANG Hongling; WU Xiaoyan; LI Chuanyou

    2005-01-01

    The wound-inducible expression of proteinase inhibitors (PIs) genes in tomato provides a powerful model system to elucidate the signal transduction pathway of sys- temic defense response. An increasing body of evidence indi- cates that systemin and jasmonic acid (JA) work in the same signaling pathway to activate the expression of PIs and other defense-related genes. However, little is known about how systemin and JA interact to regulate cell to cell communica- tion over long distances. Genetic analysis of the systemin/JA signaling pathway in tomato plants provides a unique opportunity to dissect the mechanism by which peptide and oxylipin signals interact to coordinate systemic expression of defense-related genes. Previously, it has been proposed that systemin is the long-distance mobile signal for systemic expression of defense related genes. However, recent genetic approach provided new evidence that jasmonic acid, rather than systemin, functions as the systemic wound signal, and that the peptide systemin works to regulate the biosynthesis of JA.

  6. Integrated cellular systems

    Science.gov (United States)

    Harper, Jason C.

    integrate cells and direct their behaviors. This process permits, for the first time, the selection and in situ isolation of a single target cell from a population of cells with mixed phenotypes, and the subsequent monitoring of its behavior, and that of its progeny, under well defined conditions. These techniques promise a new means to integrate biomolecules with nanostructures and macroscale systems, and to manipulate cellular behavior at the individual cell level, having significant implications towards development of practical and robust integrated cellular systems.

  7. Ethanol Cellular Defense Induce Unfolded Protein Response in Yeast.

    Science.gov (United States)

    Navarro-Tapia, Elisabet; Nana, Rebeca K; Querol, Amparo; Pérez-Torrado, Roberto

    2016-01-01

    Ethanol is a valuable industrial product and a common metabolite used by many cell types. However, this molecule produces high levels of cytotoxicity affecting cellular performance at several levels. In the presence of ethanol, cells must adjust some of their components, such as the membrane lipids to maintain homeostasis. In the case of microorganism as Saccharomyces cerevisiae, ethanol is one of the principal products of their metabolism and is the main stress factor during fermentation. Although, many efforts have been made, mechanisms of ethanol tolerance are not fully understood and very little evidence is available to date for specific signaling by ethanol in the cell. This work studied two S. cerevisiae strains, CECT10094, and Temohaya-MI26, isolated from flor wine and agave fermentation (a traditional fermentation from Mexico) respectively, which differ in ethanol tolerance, in order to understand the molecular mechanisms underlying the ethanol stress response and the reasons for different ethanol tolerance. The transcriptome was analyzed after ethanol stress and, among others, an increased activation of genes related with the unfolded protein response (UPR) and its transcription factor, Hac1p, was observed in the tolerant strain CECT10094. We observed that this strain also resist more UPR agents than Temohaya-MI26 and the UPR-ethanol stress correlation was corroborated observing growth of 15 more strains and discarding UPR correlation with other stresses as thermal or oxidative stress. Furthermore, higher activation of UPR pathway in the tolerant strain CECT10094 was observed using a UPR mCherry reporter. Finally, we observed UPR activation in response to ethanol stress in other S. cerevisiae ethanol tolerant strains as the wine strains T73 and EC1118. This work demonstrates that the UPR pathway is activated under ethanol stress occurring in a standard fermentation and links this response to an enhanced ethanol tolerance. Thus, our data suggest that there

  8. Ethanol cellular defense induce unfolded protein response in yeast

    Directory of Open Access Journals (Sweden)

    Elisabet eNavarro-Tapia

    2016-02-01

    Full Text Available Ethanol is a valuable industrial product and a common metabolite used by many cell types. However, this molecule produces high levels of cytotoxicity affecting cellular performance at several levels. In the presence of ethanol, cells must adjust some of their components, such as the membrane lipids to maintain homeostasis. In the case of microorganism as Saccharomyces cerevisiae, ethanol is one of the principal products of their metabolism and is the main stress factor during fermentation. Although many efforts have been made, mechanisms of ethanol tolerance are not fully understood and very little evidence is available to date for specific signaling by ethanol in the cell. This work studied two Saccharomyces cerevisiae strains, CECT10094 and Temohaya-MI26, isolated from flor wine and agave fermentation (a traditional fermentation from Mexico respectively, which differ in ethanol tolerance, in order to understand the molecular mechanisms underlying the ethanol stress response and the reasons for different ethanol tolerance. The transcriptome was analyzed after ethanol stress and, among others, an increased activation of genes related with the unfolded protein response (UPR and its transcription factor, Hac1p, was observed in the tolerant strain CECT10094. We observed that this strain also resist more UPR agents than Temohaya-MI26 and the UPR-ethanol stress correlation was corroborated observing growth of 15 more strains and discarding UPR correlation with other stresses as thermal or oxidative stress. Furthermore, higher activation of UPR pathway in the tolerant strain CECT10094 was observed using a UPR mCherry reporter. Finally, we observed UPR activation in response to ethanol stress in other S. cerevisiae ethanol tolerant strains as the wine strains T73 and EC1118. This work demonstrates that the UPR pathway is activated under ethanol stress occurring in a standard fermentation and links this response to an enhanced ethanol tolerance. Thus

  9. Cellular-based preemption system

    Science.gov (United States)

    Bachelder, Aaron D. (Inventor)

    2011-01-01

    A cellular-based preemption system that uses existing cellular infrastructure to transmit preemption related data to allow safe passage of emergency vehicles through one or more intersections. A cellular unit in an emergency vehicle is used to generate position reports that are transmitted to the one or more intersections during an emergency response. Based on this position data, the one or more intersections calculate an estimated time of arrival (ETA) of the emergency vehicle, and transmit preemption commands to traffic signals at the intersections based on the calculated ETA. Additional techniques may be used for refining the position reports, ETA calculations, and the like. Such techniques include, without limitation, statistical preemption, map-matching, dead-reckoning, augmented navigation, and/or preemption optimization techniques, all of which are described in further detail in the above-referenced patent applications.

  10. Defense system shortcuts and limits of scope.

    Science.gov (United States)

    Rewald, E; Francischetti, M M

    2000-10-01

    Defense, as a key factor of life, shares the biological tendencies of simplicity and energy saving. We propose that, like the mind, defense tends to rely on shortcuts via immune memes. Also, response repetition may induce the formation of virtual 'modules' [toolkits] to simplify and perfect performance. Engaged modules may expand by proliferating or by capturing immune components from the 'dormant' and even perhaps from active ones. With regard to recovery and/or survival, complexity of the integrated defense system (IDS) (1) requires to be inside of what we call the 'functional window'. In contrast to the physiological and common disease repair, energy is squandered when IDS perceives real danger. Our concern is the uncertain transition to conditions that do not fit into the IDS routine and, even worse, that are outside the functional window where the system is lacking. PMID:11000052

  11. Intelligent systems for strategic power infrastructure defense

    Science.gov (United States)

    Jung, Ju-Hwan

    A fault or disturbance in a power system can be severe due to the sources of vulnerability such as human errors, protection and control system failures, a failure of communication networks to deliver critical control signals, and market and load uncertainties. There have been several catastrophic failures resulting from disturbances involving the sources of vulnerability while power systems are designed to withstand disturbances or faults. To avoid catastrophic failures or minimize the impact of a disturbance(s), the state of the power system has to be analyzed correctly and preventive or corrective self-healing control actions have to be deployed. This dissertation addresses two aspects of power systems: Defense system and diagnosis, both concerned with the power system analysis and operation during events involving faults or disturbances. This study is intended to develop a defense system that is able to assess power system vulnerability and to perform self-healing control actions based on the system-wide analysis. In order to meet the requirements of the system-wide analysis, the defense system is designed with multi-agent system technologies. Since power systems are dynamic and uncertain the self-healing control actions need to be adaptive. This study applies the reinforcement learning technique to provide a theoretical basis for adaptation. One of the important issues in adaptation is the convergence of the learning algorithm. An appropriate convergence criterion is derived and an application with a load-shedding scheme is demonstrated in this study. This dissertation also demonstrates the feasibility of the defense system and self-healing control actions through multi-agent system technologies. The other subject of this research is to investigate the methodology for on-line fault diagnosis using the information from Sequence-of-Events Recorders (SER). The proposed multiple-hypothesis analysis generates one or more hypothetical fault scenarios to interpret the

  12. Analysis of cellular manufacturing systems

    OpenAIRE

    Heragu, Sunderesh; Meng, Gang; Zijm, Henk; Ommeren, van, J.C.

    2001-01-01

    In this paper, we present an open queuing network modeling approach to estimate performance measures of a cellular manufacturing layout. It is assumed a layout and production data for a planning period of specified length are available. The production data takes into account, processing and handling set-up times as well as transfer and process batch size information of multiple products that flow through the system. It is assumed that two sets of discrete material handling devices are used fo...

  13. Adult Drosophila melanogaster evolved for antibacterial defense invest in infection-induced expression of both humoral and cellular immunity genes

    Directory of Open Access Journals (Sweden)

    McGraw Elizabeth A

    2011-08-01

    Full Text Available Abstract Background While the transcription of innate immunity genes in response to bacterial infection has been well-characterised in the Drosophila model, we recently demonstrated the capacity for such transcription to evolve in flies selected for improved antibacterial defense. Here we use this experimental system to examine how insects invest in constitutive versus infection-induced transcription of immunity genes. These two strategies carry with them different consequences with respect to energetic and pleiotropic costs and may be more or less effective in improving defense depending on whether the genes contribute to humoral or cellular aspects of immunity. Findings Contrary to expectation we show that selection preferentially increased the infection-induced expression of both cellular and humoral immunity genes. Given their functional roles, infection induced increases in expression were expected for the humoral genes, while increases in constitutive expression were expected for the cellular genes. We also report a restricted ability to improve transcription of immunity genes that is on the order of 2-3 fold regardless of total transcription level of the gene. Conclusions The evolved increases in infection-induced expression of the cellular genes may result from specific cross talk with humoral pathways or from generalised strategies for enhancing immunity gene transcription. A failure to see improvements in constitutive expression of the cellular genes suggests either that increases might come at too great a cost or that patterns of expression in adults are decoupled from the larval phase where increases would be most effective. The similarity in fold change increase across all immunity genes may suggest a shared mechanism for the evolution of increased transcription in small, discrete units such as duplication of cis-regulatory elements.

  14. DEFENSE MECHANISMS FOR COMPUTER-BASED INFORMATION SYSTEMS

    OpenAIRE

    Majid Alshammari; Christian Bach

    2013-01-01

    Nowadays, corporations and a government agencies relay on computer-based information system to manage their information, this information may be classified, so it will be dangerous if it is disclosed by unauthorized persons. Therefore, there is urgent need for defense. In this research, defense has been categorized into four mechanisms technical defense, operation defense, management defense, and physical defense based on the logic of computer and network security. Also, each mechanism has be...

  15. Immediate–Early(IE) gene regulation of cytomegalovirus:IE1-and pp71-mediated viral strategies against cellular defenses

    Institute of Scientific and Technical Information of China (English)

    Lilith; Torres; Qiyi; Tang

    2014-01-01

    Three crucial hurdles hinder studies on human cytomegalovirus(HCMV): strict species specificity, differences between in vivo and in vitro infection, and the complexity of gene regulation. Ever since the sequencing of the whole genome was first accomplished, functional studies on individual genes have been the mainstream in the CMV field. Gene regulation has therefore been elucidated in a more detailed fashion. However, viral gene regulation is largely controlled by both cellular and viral components. In other words, viral gene expression is determined by the virus–host interaction. Generally, cells respond to viral infection in a defensive pattern; at the same time, viruses try to counteract the cellular defense or else hide in the host(latency). Viruses evolve effective strategies against cellular defense in order to achieve replicative success. Whether or not they are successful, cellular defenses remain in the whole viral replication cycle: entry, immediate–early(IE) gene expression, early gene expression, DNA replication, late gene expression, and viral egress. Many viral strategies against cellular defense, and which occur in the immediate–early time of viral infection, have been documented. In this review, we will summarize the documented biological functions of IE1 and pp71 proteins, especially with regard to how they counteract cellular intrinsic defenses.

  16. Relative roles of the cellular and humoral responses in the Drosophila host defense against three gram-positive bacterial infections.

    Directory of Open Access Journals (Sweden)

    Nadine T Nehme

    Full Text Available BACKGROUND: Two NF-kappaB signaling pathways, Toll and immune deficiency (imd, are required for survival to bacterial infections in Drosophila. In response to septic injury, these pathways mediate rapid transcriptional activation of distinct sets of effector molecules, including antimicrobial peptides, which are important components of a humoral defense response. However, it is less clear to what extent macrophage-like hemocytes contribute to host defense. METHODOLOGY/PRINCIPAL FINDINGS: In order to dissect the relative importance of humoral and cellular defenses after septic injury with three different gram-positive bacteria (Micrococcus luteus, Enterococcus faecalis, Staphylococcus aureus, we used latex bead pre-injection to ablate macrophage function in flies wildtype or mutant for various Toll and imd pathway components. We found that in all three infection models a compromised phagocytic system impaired fly survival--independently of concomitant Toll or imd pathway activation. Our data failed to confirm a role of the PGRP-SA and GNBP1 Pattern Recognition Receptors for phagocytosis of S. aureus. The Drosophila scavenger receptor Eater mediates the phagocytosis by hemocytes or S2 cells of E. faecalis and S. aureus, but not of M. luteus. In the case of M. luteus and E. faecalis, but not S. aureus, decreased survival due to defective phagocytosis could be compensated for by genetically enhancing the humoral immune response. CONCLUSIONS/SIGNIFICANCE: Our results underscore the fundamental importance of both cellular and humoral mechanisms in Drosophila immunity and shed light on the balance between these two arms of host defense depending on the invading pathogen.

  17. Estimation in Cellular Radio Systems

    OpenAIRE

    Blom, Jonas; Gunnarsson, Fredrik; Gustafsson, Fredrik

    1999-01-01

    The problem to track time-varying parameters in cellular radio systems is studied, and the focus is on estimation based only on the signals that are readily available. Previous work have demonstrated very good performance, but were relying on analog measurement that are not available. Most of the information is lost due to quantization and sampling at a rate that might be as low as 2 Hz (GSM case). For that matter a maximum likelihood estimator have been designed and exemplified in the case o...

  18. A Hierarchal System of Defense Mechanisms.

    Science.gov (United States)

    Lee, Gerald

    1979-01-01

    Constructs a hierarchy of defense mechanisms through the addition of the concepts of secondary and tertiary defenses to the already existing psychoanalytic defenses and increases the explanatory power of the concept of defense. Research generated from this theoretical model is presented. (Author/BEF)

  19. Cellular structure in system of interacting particles

    OpenAIRE

    Lev, Bohdan

    2008-01-01

    The general description of formation the cellular structure in the system of interacting particles is proposed. Interactions between particles are presumably well-understood and the phase transition in which can be studied in the scale of particle resolution. We presented analytical results of possible cellular structures for suspension of colloidal particles, in system particles immersed in liquid crystal and gravitational system. We have shown that cellular structure formation can occur in ...

  20. Recent development of cellular manufacturing systems

    Indian Academy of Sciences (India)

    P K Arora; A Haleem; M K Singh

    2013-06-01

    Cellular manufacturing system has been proved a vital approach for batch and job shop production systems. Group technology has been an essential tool for developing a cellular manufacturing system. The paper aims to discuss various cell formation techniques and highlights the significant research work done in past over the years and attempts to points out the gap in research.

  1. Analysis of cellular manufacturing systems

    NARCIS (Netherlands)

    Heragu, Sunderesh; Meng, Gang; Zijm, Henk; Ommeren, van Jan-Kees

    2001-01-01

    In this paper, we present an open queuing network modeling approach to estimate performance measures of a cellular manufacturing layout. It is assumed a layout and production data for a planning period of specified length are available. The production data takes into account, processing and handli

  2. Comparative genomics of defense systems in archaea and bacteria

    OpenAIRE

    Makarova, Kira S.; Wolf, Yuri I.; Koonin, Eugene V.

    2013-01-01

    Our knowledge of prokaryotic defense systems has vastly expanded as the result of comparative genomic analysis, followed by experimental validation. This expansion is both quantitative, including the discovery of diverse new examples of known types of defense systems, such as restriction-modification or toxin-antitoxin systems, and qualitative, including the discovery of fundamentally new defense mechanisms, such as the CRISPR-Cas immunity system. Large-scale statistical analysis reveals that...

  3. The Universe as a Cellular System

    CERN Document Server

    Aragón-Calvo, Miguel A

    2014-01-01

    Cellular systems are observed everywhere in nature, from crystal domains in metals, soap froth and cucumber cells to the network of cosmological voids. Surprisingly, despite their disparate scale and origin all cellular systems follow certain scaling laws relating their geometry, topology and dynamics. Using a cosmological N-body simulation we found that the Cosmic Web, the largest known cellular system, follows the same scaling relations seen elsewhere in nature. Our results extend the validity of scaling relations in cellular systems by over 30 orders of magnitude in scale with respect to previous studies. The dynamics of cellular systems can be used to interpret local observations such as the local velocity anomaly as the result of a collapsing void in our cosmic backyard. Moreover, scaling relations depend on the curvature of space, providing an independent measure of geometry.

  4. THE DEFENSE PLANNING SYSTEMS AND THEIR IMPLICATIONS

    Directory of Open Access Journals (Sweden)

    Laszlo STICZ

    2010-01-01

    Full Text Available Defense planning in the Alliance is a fundamental element of the arrangements which enable its member countries to enjoy the crucial political, military and resource advantages of collective defense and other common military efforts to enhance security and stability. In this respect, the aim of this paper is to outline the role of the Armed Forces and the specific processes aiming to achieve the ultimate goal of a nation regarding national security, with focus on defense planning and the PDPS.

  5. MILLIMETER-WAVE EMISSIVITY OF CELLULAR SYSTEMS

    Science.gov (United States)

    A general analysis has been presented of the millimeter-wave and farinfrared spectroscopic properties of in vivo cellular systems, and of the boson radiative equilibrium with steady-state nonequilibrium molecular systems. The frequency threshhold of spectroscopic properties assoc...

  6. Antioxidant defense system in hibernating mammals

    Directory of Open Access Journals (Sweden)

    Antonova Ekaterina Petrovna

    2015-06-01

    Full Text Available Hibernation is one of the most striking examples of mammals’ phenotypic flexibility, allowing them to survive at low temperatures and in the conditions of insufficient food and water. During the torpor there is a decrease in both body temperature and metabolic rate that is accompanied by slowing respiration, significant drop of oxygen consumption as well as by the reduction of cerebral blood circulation and heart rate. In spite of a number of physiological adaptations to hibernation, the arousal from torpor is accompanied by the oxidative stress caused by the enormous rise of oxygen consumption. In these conditions, the antioxidant defense system which provides harmless transfer from torpor to arousal and vice versa, i.e., without any tissues’ oxidative damage, becomes especially important. Up to date, it is not fully elucidated how the antioxidant system acts during the periods of hibernation, but it deserves careful attention. In the review the information about the use of different strategies of antioxidant protection during hibernation in small mammals was collected

  7. Deception used for Cyber Defense of Control Systems

    Energy Technology Data Exchange (ETDEWEB)

    Wayne F. Boyer; Miles A. McQueen

    2009-05-01

    Control system cyber security defense mechanisms may employ deception to make it more difficult for attackers to plan and execute successful attacks. These deceptive defense mechanisms are organized and initially explored according to a specific deception taxonomy and the seven abstract dimensions of security previously proposed as a framework for the cyber security of control systems.

  8. Weighted Centroid Correction Localization in Cellular Systems

    Directory of Open Access Journals (Sweden)

    Rong-Zheng Li

    2011-01-01

    Full Text Available Problem statement: There is a large demand for wireless Location-Based Service (LBS and it is provided by many wireless cellular systems. In process of positioning a Mobile Station (MS, the computing speed is as important as the positioning accuracy and the algorithm should also be resistant to environmental influences. Approach: A new positioning method based on Weighted Centroid Correction Localization (WCCL for wireless cellular systems is introduced in this article. Firstly, referring to the receiving-state of an MS in cellular systems, it computes a weighted centroid of surrounding Base Stations (BSs as a rough approximate position of the MS. Then, according to the distances between the MS and the BSs being less or bigger than the computed distances between the BSs and the weighted centroid, it corrects the coordinate of the weighted centroid towards the directions of the BSs by moving it closer or farther in turn. Results: According to our experiments, WCCL improves the positioning accuracy, as well as to provide a better resistance to environmental influences. Conclusion: As a modified centroid-based localization algorithm, WCCL obtains weighting factors from the receiving-state of MS in multi-cells structured cellular systems and obtains a better positioning result in cellular systems without updating the network equipment. Therefore, for the cellular positioning problem, WCCL algorithm can be an alternate solution.

  9. Cellular computation using classifier systems

    OpenAIRE

    Kelly, Ciaran; Decraene, James, Lobo, Victor; Mitchell, George G.; McMullin, Barry; O'Brien, Darragh

    2006-01-01

    The EU FP6 Integrated Project PACE ('Programmable Artificial Cell Evolution') is investigating the creation, de novo, of chemical 'protocells'. These will be minimal 'wetware' chemical systems integrating molecular information carriers, primitive energy conversion (metabolism) and containment (membrane). Ultimately they should be capable of autonomous reproduction, and be 'programmable' to realise specific desired function. A key objective of PACE is to explore the application of such pro...

  10. Whole body exposure to low-dose γ-radiation enhances the antioxidant defense system

    International Nuclear Information System (INIS)

    It is believed that the extent of cellular damage by low- radiation dose is proportional to the effects observed at high radiation dose as per the Linear-No-Threshold (LNT) hypothesis. However, this notion may not be true at low-dose radiation exposure in the living system. Recent evidence suggest that the living organisms do not respond to ionizing radiations in a linear manner in the low dose range 0.01-0.5Gy and rather restore the homeostasis both in vivo and in vitro by normal physiological mechanisms such as cellular and DNA repair processes, immune reactions, antioxidant defense, adaptive responses, activation of immune functions, stimulation of growth etc. In this study, we have attempted to find the critical radiation dose range and the post irradiation period during which the antioxidant defense systems in the lungs, liver and kidneys remain stimulated in these organs after whole body exposure of the animals to low-dose radiation

  11. Hierarchical Cellular Structures in High-Capacity Cellular Communication Systems

    CERN Document Server

    Jain, R K; Agrawal, N K

    2011-01-01

    In the prevailing cellular environment, it is important to provide the resources for the fluctuating traffic demand exactly in the place and at the time where and when they are needed. In this paper, we explored the ability of hierarchical cellular structures with inter layer reuse to increase the capacity of mobile communication network by applying total frequency hopping (T-FH) and adaptive frequency allocation (AFA) as a strategy to reuse the macro and micro cell resources without frequency planning in indoor pico cells [11]. The practical aspects for designing macro- micro cellular overlays in the existing big urban areas are also explained [4]. Femto cells are inducted in macro / micro / pico cells hierarchical structure to achieve the required QoS cost effectively.

  12. Viral RNA Silencing Suppressors (RSS): Novel Strategy of Viruses to Ablate the Host RNA Interference (RNAi) Defense System

    OpenAIRE

    Bivalkar-Mehla, Shalmali; Vakharia, Janaki; Mehla, Rajeev; Abreha, Measho; Kanwar, Jagat Rakesh; Tikoo, Akshay; Chauhan, Ashok

    2010-01-01

    Pathogenic viruses have developed a molecular defense arsenal for their survival by counteracting the host anti-viral system known as RNA interference (RNAi). Cellular RNAi, in addition to regulating gene expression through microRNAs, also serves as a barrier against invasive foreign nucleic acids. RNAi is conserved across the biological species, including plants, animals and invertebrates. Viruses in turn, have evolved mechanisms that can counteract this anti-viral defense of the host. Recen...

  13. Literature Review on Dynamic Cellular Manufacturing System

    Science.gov (United States)

    Nouri Houshyar, A.; Leman, Z.; Pakzad Moghadam, H.; Ariffin, M. K. A. M.; Ismail, N.; Iranmanesh, H.

    2014-06-01

    In previous decades, manufacturers faced a lot of challenges because of globalization and high competition in markets. These problems arise from shortening product life cycle, rapid variation in demand of products, and also rapid changes in manufcaturing technologies. Nowadays most manufacturing companies expend considerable attention for improving flexibility and responsiveness in order to overcome these kinds of problems and also meet customer's needs. By considering the trend toward the shorter product life cycle, the manufacturing environment is towards manufacturing a wide variety of parts in small batches [1]. One of the major techniques which are applied for improving manufacturing competitiveness is Cellular Manufacturing System (CMS). CMS is type of manufacturing system which tries to combine flexibility of job shop and also productivity of flow shop. In addition, Dynamic cellular manufacturing system which considers different time periods for the manufacturing system becomes an important topic and attracts a lot of attention to itself. Therefore, this paper made attempt to have a brief review on this issue and focused on all published paper on this subject. Although, this topic gains a lot of attention to itself during these years, none of previous researchers focused on reviewing the literature of that which can be helpful and useful for other researchers who intend to do the research on this topic. Therefore, this paper is the first study which has focused and reviewed the literature of dynamic cellular manufacturing system.

  14. Defense High Level Waste Disposal Container System Description Document

    Energy Technology Data Exchange (ETDEWEB)

    N. E. Pettit

    2001-07-13

    The Defense High Level Waste Disposal Container System supports the confinement and isolation of waste within the Engineered Barrier System of the Monitored Geologic Repository (MGR). Disposal containers are loaded and sealed in the surface waste handling facilities, transferred to the underground through the accesses using a rail mounted transporter, and emplaced in emplacement drifts. The defense high level waste (HLW) disposal container provides long-term confinement of the commercial HLW and defense HLW (including immobilized plutonium waste forms [IPWF]) placed within disposable canisters, and withstands the loading, transfer, emplacement, and retrieval loads and environments. US Department of Energy (DOE)-owned spent nuclear fuel (SNF) in disposable canisters may also be placed in a defense HLW disposal container along with commercial HLW waste forms, which is known as co-disposal. The Defense High Level Waste Disposal Container System provides containment of waste for a designated period of time, and limits radionuclide release. The disposal container/waste package maintains the waste in a designated configuration, withstands maximum handling and rockfall loads, limits the individual canister temperatures after emplacement, resists corrosion in the expected handling and repository environments, and provides containment of waste in the event of an accident. Defense HLW disposal containers for HLW disposal will hold up to five HLW canisters. Defense HLW disposal containers for co-disposal will hold up to five HLW canisters arranged in a ring and one DOE SNF canister inserted in the center and/or one or more DOE SNF canisters displacing a HLW canister in the ring. Defense HLW disposal containers also will hold two Multi-Canister Overpacks (MCOs) and two HLW canisters in one disposal container. The disposal container will include outer and inner cylinders, outer and inner cylinder lids, and may include a canister guide. An exterior label will provide a means by

  15. A framework for understanding cellular manufacturing systems

    OpenAIRE

    Silva, Sílvio Carmo; Alves, Anabela Carvalho

    2002-01-01

    Many practical benefits, such as superior quality of products and short manufacturing lead times, are usually associated with Cellular Manufacturing. These and other benefits can lead to important competitive advantages of companies. However, to fully achieve these benefits there is a need for an evolution from the traditional concept of CM to the more comprehensive one, which we call Product Oriented Manufacturing. Here systems are dynamically reconfigured for total manufac...

  16. Spectrum sharing for future mobile cellular systems

    OpenAIRE

    Bennis, M

    2009-01-01

    Abstract Spectrum sharing has become a high priority research area over the past few years. The motivation behind this lies in the fact that the limited spectrum is currently inefficiently utilized. As recognized by the World radio communication conference (WRC)-07, the amount of identified spectrum is not large enough to support large bandwidths for a substantial number of operators. Therefore, it is paramount for future mobile cellular systems to share the frequency spectrum and coexist ...

  17. Systemin/Jasmonate-Mediated Systemic Defense Signaling in Tomato

    Institute of Scientific and Technical Information of China (English)

    Jia-Qiang Sun; Hong-Ling Jiang; Chuan-You Li

    2011-01-01

    Wound-inducible proteinase inhibitors (Pis)in tomato plants provide a useful model system to elucidate the signal transduction pathways that regulate systemic defense response. Among the proposed intercellular signals for wound-induced Pis expression are the peptide systemin and the oxylipin-derived phytohormone jasmonic acid (JA).An increasing body of evidence indicates that systemin and JA work in the same signaling pathway to activate the ex-pression of Pis and other defense-related genes. However, relatively less is known about how these signals interact to promote cell-to-cell communication over long distances. Genetic analysis of the systemin/JA signaling pathway in tomato plants provides a unique opportunity to study, in a single experimental system, the mechanism by which peptide and oxylipin signals interact to coordinate systemic expression of defense-related genes. Previously, it has been proposed that systemin is the long-distance mobile signal for defense gene expression. Recently, grafting experiments with tomato mutants defective in JA biosynthesis and signaling provide new evidence that JA, rather than systemin, functions as the systemic wound signal, and that the biosynthesis of JA is regulated by the peptide systemin. Further understanding of the systemin/JA signaling pathway promises to provide new insights into the basic mechanisms governing plant de-fense to biotic stress.

  18. Dynamic Channel Allocation in Sectored Cellular Systems

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    It is known that dynamic channel assignment(DCA) strategy outperforms the fixed channel assignment(FCA) strategy in omni-directional antenna cellular systems. One of the most important methods used in DCA was channel borrowing. But with the emergence of cell sectorization and spatial division multiple access(SDMA) which are used to increase the capacity of cellular systems, the channel assignment faces a series of new problems. In this paper, a dynamic channel allocation scheme based on sectored cellular systems is proposed. By introducing intra-cell channel borrowing (borrowing channels from neighboring sectors) and inter-cell channel borrowing (borrowing channels from neighboring cells) methods, previous DCA strategies, including compact pattern based channel borrowing(CPCB) and greedy based dynamic channel assignment(GDCA) schemes proposed by the author, are improved significantly. The computer simulation shows that either intra-cell borrowing scheme or inter-cell borrowing scheme is efficient enough to uniform and non-uniform traffic service distributions.

  19. 47 CFR 22.923 - Cellular system configuration.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Cellular system configuration. 22.923 Section... MOBILE SERVICES Cellular Radiotelephone Service § 22.923 Cellular system configuration. Mobile stations... directly or through cellular repeaters. Auxiliary test stations may communicate with base or...

  20. Multiple operating system rotation environment moving target defense

    Science.gov (United States)

    Evans, Nathaniel; Thompson, Michael

    2016-03-22

    Systems and methods for providing a multiple operating system rotation environment ("MORE") moving target defense ("MTD") computing system are described. The MORE-MTD system provides enhanced computer system security through a rotation of multiple operating systems. The MORE-MTD system increases attacker uncertainty, increases the cost of attacking the system, reduces the likelihood of an attacker locating a vulnerability, and reduces the exposure time of any located vulnerability. The MORE-MTD environment is effectuated by rotation of the operating systems at a given interval. The rotating operating systems create a consistently changing attack surface for remote attackers.

  1. Traffic Engineering of Cellular Wireless Communication Systems

    DEFF Research Database (Denmark)

    Iversen, Villy Bæk

    2002-01-01

    In mobile communications an efficient utilisation of the channels is of great importance. In this paper we describe the basic principles for obtaining the maximum utilisation and study strategies for obtaining these limits. In general a high degree of sharing is efficient, but requires service...... protection mechanisms to guarantee the Quality of Service for all services. We study cellular systems with hierarchical cells, and the effect of overlapping cells, and we show that by call packing we obtain a very high utilisation. The models are generalisations of the Erlang-B formula, and include general...

  2. System defense mechanisms in nuclear power generation control computers

    International Nuclear Information System (INIS)

    Dual-redundant computers are used to control and monitor the production of power from Ontario Hydro's nuclear power reactors. Each computer must have the capability of monitoring its own performance and detecting faults. Upon fault detection, each computer must initiate corrective responses to ensure the integrity of critical control functions. This paper reviews those features the authors have termed system defense mechanisms

  3. Defense High Level Waste Disposal Container System Description

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2000-10-12

    The Defense High Level Waste Disposal Container System supports the confinement and isolation of waste within the Engineered Barrier System of the Monitored Geologic Repository (MGR). Disposal containers are loaded and sealed in the surface waste handling facilities, transferred to the underground through the accesses using a rail mounted transporter, and emplaced in emplacement drifts. The defense high level waste (HLW) disposal container provides long-term confinement of the commercial HLW and defense HLW (including immobilized plutonium waste forms (IPWF)) placed within disposable canisters, and withstands the loading, transfer, emplacement, and retrieval loads and environments. U.S. Department of Energy (DOE)-owned spent nuclear fuel (SNF) in disposable canisters may also be placed in a defense HLW disposal container along with commercial HLW waste forms, which is known as 'co-disposal'. The Defense High Level Waste Disposal Container System provides containment of waste for a designated period of time, and limits radionuclide release. The disposal container/waste package maintains the waste in a designated configuration, withstands maximum handling and rockfall loads, limits the individual canister temperatures after emplacement, resists corrosion in the expected handling and repository environments, and provides containment of waste in the event of an accident. Defense HLW disposal containers for HLW disposal will hold up to five HLW canisters. Defense HLW disposal containers for co-disposal will hold up to five HLW canisters arranged in a ring and one DOE SNF canister in the ring. Defense HLW disposal containers also will hold two Multi-Canister Overpacks (MCOs) and two HLW canisters in one disposal container. The disposal container will include outer and inner cylinders, outer and inner cylinder lids, and may include a canister guide. An exterior label will provide a means by which to identify the disposal container and its contents. Different

  4. The natural defense system and the normative self model.

    Science.gov (United States)

    Kourilsky, Philippe

    2016-01-01

    Infectious agents are not the only agressors, and the immune system is not the sole defender of the organism. In an enlarged perspective, the 'normative self model' postulates that a 'natural defense system' protects man and other complex organisms against the environmental and internal hazards of life, including infections and cancers. It involves multiple error detection and correction mechanisms that confer robustness to the body at all levels of its organization. According to the model, the self relies on a set of physiological norms, and NONself (meaning : Non Obedient to the Norms of the self) is anything 'off-norms'. The natural defense system comprises a set of 'civil defenses' (to which all cells in organs and tissues contribute), and a 'professional army ', made of a smaller set of mobile cells. Mobile and non mobile cells differ in their tuning abilities. Tuning extends the recognition capabilities of NONself by the mobile cells, which increase their defensive function. To prevent them to drift, which would compromise self/NONself discrimination, the more plastic mobile cells need to periodically refer to the more stable non mobile cells to keep within physiological standards. PMID:27303629

  5. Defense against common-mode failures in protection system design

    International Nuclear Information System (INIS)

    The introduction of digital instrumentation and control into reactor safety systems creates a heightened concern about common-mode failure. This paper discusses the concern and methods to cope with the concern. Common-mode failures have been a ''fact-of-life'' in existing systems. The informal introduction of defense-in-depth and diversity (D-in-D ampersand D)-coupled with the fact that hardware common-mode failures are often distributed in time-has allowed systems to deal with past common-mode failures. However, identical software operating in identical redundant systems presents the potential for simultaneous failure. Consequently, the use of digital systems raises the concern about common-mode failure to a new level. A more methodical approach to mitigating common-mode failure is needed to address these concerns. Purposeful introduction of D-in-D ampersand D has been used as a defense against common-mode failure in reactor protection systems. At least two diverse systems are provided to mitigate any potential initiating event. Additionally, diverse displays and controls are provided to allow the operator to monitor plant status and manually initiate engineered safety features. A special form of conimon-mode failure analysis called ''defense-in-depth and diversity analysis'' has been developed to identify possible conimon-mode failure vulnerabilities in digital systems. An overview of this analysis technique is provided

  6. Defense and attack of complex and dependent systems

    International Nuclear Information System (INIS)

    A framework is constructed for how to analyze the strategic defense of an infrastructure subject to attack by a strategic attacker. Merging operations research, reliability theory, and game theory for optimal analytical impact, the optimization program for the defender and attacker is specified. Targets can be in parallel, series, combined series-parallel, complex, k-out-of-n redundancy, independent, interdependent, and dependent. The defender and attacker determine how much to invest in defending versus attacking each of multiple targets. A target can have economic, human, and symbolic values, subjectively assessed by the defender and attacker. A contest success function determines the probability of a successful attack on each target, dependent on the investments by the defender and attacker into each target, and on characteristics of the contest. The defender minimizes the expected damage plus the defense costs. The attacker maximizes the expected damage minus the attack costs. Each agent is concerned about how his investments vary across the targets, and the impact on his utilities. Interdependent systems are analyzed where the defense and attack on one target impacts all targets. Dependent systems are analyzed applying Markov analysis and repeated games where a successful attack on one target in the first period impacts the unit costs of defense and attack, and the contest intensity, for the other target in the second period.

  7. Simulating Complex Systems by Cellular Automata

    CERN Document Server

    Kroc, Jiri; Hoekstra, Alfons G

    2010-01-01

    Deeply rooted in fundamental research in Mathematics and Computer Science, Cellular Automata (CA) are recognized as an intuitive modeling paradigm for Complex Systems. Already very basic CA, with extremely simple micro dynamics such as the Game of Life, show an almost endless display of complex emergent behavior. Conversely, CA can also be designed to produce a desired emergent behavior, using either theoretical methodologies or evolutionary techniques. Meanwhile, beyond the original realm of applications - Physics, Computer Science, and Mathematics – CA have also become work horses in very different disciplines such as epidemiology, immunology, sociology, and finance. In this context of fast and impressive progress, spurred further by the enormous attraction these topics have on students, this book emerges as a welcome overview of the field for its practitioners, as well as a good starting point for detailed study on the graduate and post-graduate level. The book contains three parts, two major parts on th...

  8. REQUIREMENTS FOR SYSTEMS DEVELOPMENT LIFE CYCLE MODELS FOR LARGE-SCALE DEFENSE SYSTEMS

    Directory of Open Access Journals (Sweden)

    Kadir Alpaslan DEMIR

    2015-10-01

    Full Text Available TLarge-scale defense system projects are strategic for maintaining and increasing the national defense capability. Therefore, governments spend billions of dollars in the acquisition and development of large-scale defense systems. The scale of defense systems is always increasing and the costs to build them are skyrocketing. Today, defense systems are software intensive and they are either a system of systems or a part of it. Historically, the project performances observed in the development of these systems have been signifi cantly poor when compared to other types of projects. It is obvious that the currently used systems development life cycle models are insuffi cient to address today’s challenges of building these systems. Using a systems development life cycle model that is specifi cally designed for largescale defense system developments and is effective in dealing with today’s and near-future challenges will help to improve project performances. The fi rst step in the development a large-scale defense systems development life cycle model is the identifi cation of requirements for such a model. This paper contributes to the body of literature in the fi eld by providing a set of requirements for system development life cycle models for large-scale defense systems. Furthermore, a research agenda is proposed.

  9. Optimization of Inter Cellular Movement of Parts in Cellular Manufacturing System Using Genetic Algorithm

    OpenAIRE

    Siva Prasad Darla; C.D. Naiju; Polu Vidya Sagar; B. Venkat Likhit

    2014-01-01

    In the modern manufacturing environment, Cellular Manufacturing Systems (CMS) have gained greater importance in job shop or batch-type production to gain economic advantage similar to those of mass production. Successful implementation of CMS highly depends on the determination of part families; machine cells and minimizing inter cellular movement. This study considers machine component grouping problems namely inter-cellular movement and cell load variation by developing a mathematical model...

  10. Immunity, suicide or both? Ecological determinants for the combined evolution of anti-pathogen defense systems

    OpenAIRE

    Iranzo, Jaime; Lobkovsky, Alexander E.; Wolf, Yuri I.; Koonin, Eugene V.

    2015-01-01

    Background Parasite-host arms race is one of the key factors in the evolution of life. Most cellular life forms, in particular prokaryotes, possess diverse forms of defense against pathogens including innate immunity, adaptive immunity and programmed cell death (altruistic suicide). Coevolution of these different but interacting defense strategies yields complex evolutionary regimes. Results We develop and extensively analyze a computational model of coevolution of different defense strategie...

  11. Passive automatic anti-piracy defense system of ships

    Science.gov (United States)

    Szustakowski, M.; Życzkowski, M.; Ciurapiński, W.; Karol, M.; Kastek, M.; Stachowiak, R.; Markowski, P.

    2013-10-01

    The article describes the technological solution for ship self-defense against pirate attacks. The paper presents the design solutions in the field of direct physical protection. All the solutions are connected with the latest optoelectronic and microwave systems and sensors to detect, recognize and the threat posed by pirates. In particular, tests of effectiveness and the detection-range of technology demonstrator developed by a team of authors were carried out.

  12. Pineal Proteins Upregulate Specific Antioxidant Defense Systems in the Brain

    OpenAIRE

    Vijay K. Bharti; Srivastava, R. S.

    2009-01-01

    The neuroendocrine functions of the pineal affect a wide variety of glandular and nervous system processes. Beside melatonin (MEL), the pineal gland secretes and expresses certain proteins essential for various physiological functions. It has been suggested that the pineal gland may also have an antioxidant role due to secretory product other than MEL. Therefore, the present study was designed to study the effect of buffalo (Bubalus bubalis) pineal proteins (PP) on the antioxidant defense sys...

  13. The design about the intrusion defense system for IHEP

    International Nuclear Information System (INIS)

    With the development of network technologies, limitations on traditional methods of network security protection are becoming more and more obvious. An individual network security product or the simple combination of several products can hardly complete the goal of keeping from hackers' intrusion. Therefore, on the basis of the analyses about the security problems of IHEPNET which is an open and scientific research network, the author designs an intrusion defense system especially for IHEPNET

  14. Optimization of Inter Cellular Movement of Parts in Cellular Manufacturing System Using Genetic Algorithm

    Directory of Open Access Journals (Sweden)

    Siva Prasad Darla

    2014-01-01

    Full Text Available In the modern manufacturing environment, Cellular Manufacturing Systems (CMS have gained greater importance in job shop or batch-type production to gain economic advantage similar to those of mass production. Successful implementation of CMS highly depends on the determination of part families; machine cells and minimizing inter cellular movement. This study considers machine component grouping problems namely inter-cellular movement and cell load variation by developing a mathematical model and optimizing the solution using Genetic Algorithm to arrive at a cell formation to minimize the inter-cellular movement and cell load variation. The results are presented with a numerical example.

  15. Ozone and nitrogen dioxide. A study on mechanisms of toxic action and cellular defense.

    OpenAIRE

    I.M.C.M. Rietjens

    1986-01-01

    Ozone and nitrogen dioxide are major toxic components of photochemical smog. They arise from the combustion of fossil fuels (traffic, industrial processes) and from solar radiation-catalyzed reactions in polluted atmospheres.The morphological, physiological and biochemical effects of ozone and nitrogen dioxide on the respiratory system of man and experimental animals have been investigated over the last decades.More recently the development of i) isolation and cell culture procedures for diff...

  16. 76 FR 44293 - Defense Acquisition Regulations System; Defense Federal Acquisition Regulation Supplement; Only...

    Science.gov (United States)

    2011-07-25

    ... presented by the Under Secretary of Defense for Acquisition, Technology, & Logistics in a memorandum dated... are in support of contingency, humanitarian, or peacekeeping operations, or to facilitate defense...) At or below the simplified acquisition threshold; or (ii) In support of contingency, humanitarian...

  17. Possible allied ballistic missile defense systems: Related guidance and control requirements

    Science.gov (United States)

    Roche, C.; Cotillard, C.

    1995-01-01

    This document discusses several different types of Ballistic Missile Defense Systems for NATO for both medium and long range attacks. The feasibility, technological assessment and forecasting, and defense architecture is presented, along with each system's capabilities.

  18. Control Systems Cyber Security:Defense in Depth Strategies

    Energy Technology Data Exchange (ETDEWEB)

    David Kuipers; Mark Fabro

    2006-05-01

    Information infrastructures across many public and private domains share several common attributes regarding IT deployments and data communications. This is particularly true in the control systems domain. A majority of the systems use robust architectures to enhance business and reduce costs by increasing the integration of external, business, and control system networks. However, multi-network integration strategies often lead to vulnerabilities that greatly reduce the security of an organization, and can expose mission-critical control systems to cyber threats. This document provides guidance and direction for developing ‘defense-in-depth’ strategies for organizations that use control system networks while maintaining a multi-tier information architecture that requires: Maintenance of various field devices, telemetry collection, and/or industrial-level process systems Access to facilities via remote data link or modem Public facing services for customer or corporate operations A robust business environment that requires connections among the control system domain, the external Internet, and other peer organizations.

  19. Cellular toxicity (High-Throughput Cellular Assays for Modeling Toxicity in the Fish Reproductive System)

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The purpose of this project is to adapt cellular in vitro assay systems of the rainbow trout pituitary, liver and ovary for high-throughput screening (HTS) of...

  20. Minmax defense strategy for complex multi-state systems

    International Nuclear Information System (INIS)

    This paper presents a general optimization methodology that merges game theory and multi-state system survivability theory. The defender has multiple alternatives of defense strategy that presumes separation and protection of system elements. The attacker also has multiple alternatives of its attack strategy based on a combination of different possible attack actions against different groups of system elements. The defender minimizes, and the attacker maximizes, the expected damage caused by the attack (taking into account the unreliability of system elements and the multi-state nature of complex series-parallel systems). The problem is defined as a two-period minmax non-cooperative game between the defender who moves first and the attacker who moves second. An exhaustive minmax optimization algorithm is presented based on a double-loop genetic algorithm for determining the solution. A universal generating function technique is applied for evaluating the losses caused by system performance reduction. Illustrative examples with solutions are presented

  1. Pineal Proteins Upregulate Specific Antioxidant Defense Systems in the Brain

    Directory of Open Access Journals (Sweden)

    Vijay K. Bharti

    2009-01-01

    Full Text Available The neuroendocrine functions of the pineal affect a wide variety of glandular and nervous system processes. Beside melatonin (MEL, the pineal gland secretes and expresses certain proteins essential for various physiological functions. It has been suggested that the pineal gland may also have an antioxidant role due to secretory product other than MEL. Therefore, the present study was designed to study the effect of buffalo (Bubalus bubalis pineal proteins (PP on the antioxidant defense system in the brain of female rats. The twenty-four rats were taken in present study and were divided into four groups: control (0 day, control (28 day, vehicle control and buffalo PP. The PP was injected 100 µg/kg BW intraperitoneal (i.p. daily for 28 days. The activities of superoxide dismutase (SOD, glutathione peroxidase (GPx, catalase (CAT, glutathione reductase (GR and reduced glutathione (GSH concentration and the levels of lipid peroxidation (LPO in the brain tissue were measured to assess the antioxidant systems. These enzymes protect from adverse effects of free radicals and help in amelioration of oxidative stress. Buffalo pineal proteins administration did not cause any effect on brain LPO, whereas GPx, GR and GSH were significantly (p < 0.05 decreased. However, SOD and CAT activities were increased to significant levels than the control in PP treated rats. Our study herein suggested that buffalo (Bubalus bubalis pineal proteins upregulates specific antioxidant defense systems and can be useful in control of various oxidative stress-induced neuronal diseases.

  2. a GIS of SARDINIA'S Coastal Defense System (xvi - XVIII Century)

    Science.gov (United States)

    Deidda, M.; Musa, C.; Vacca, G.

    2015-06-01

    The use of GIS as a tool for archival, analysis and representation of geographic information has become significantly popular in many scientific fields that are directly concerned with the "territory" as their object of study. The field of application of GIS, however, has expanded also in other areas, such as those related to humanities and architecture, in which the territory is studied in an "indirect" mode because it constitutes a kind of substrate on which to develop a specific spatial analysis for particular purposes. Among these areas are to be included certainly archeology and restoration, fields in which the GIS has become a useful tool for historical studies. In this work we present a GIS developed for the study of the historical and territorial coastal defense system of Sardinia (16th - 18th century), in order to respond to the need to store, analyze and efficiently manage the information regarding cultural heritage and landscape heritage such as that consisting of the coastal defensive towers of Sardinia. This defensive system, in fact, was composed by over 100 towers positioned around the entire coastal perimeter of Sardinia, of which more than 90 still exist today. Their position was planned on the basis of the following criteria: - Warning the neighboring towers about the sighting of enemy ships - Protecting coasts located near the towns - Monitoring the water sources near the coast - Allowing for the full visibility of the coasts of any morphology With this study we also verified, through the use of high resolution and high accuracy DTM (LiDAR) and the topographic databases, whether the positioning criteria specified in the design of the system were respected and effective.

  3. Ethanol Extract of Ganoderma lucidum Augments Cellular Anti-oxidant Defense through Activation of Nrf2/HO-1

    Directory of Open Access Journals (Sweden)

    Yoo-hwan Lee

    2016-03-01

    augments the cellular anti-oxidant defense capacity through activation of Nrf2/HO-1, thereby protecting C2C12 myoblasts from H2O2-induced oxidative cytotoxicity.

  4. Redesigning the Structure of Republic Indonesian Defense System; An Analysis of Systems Thinking

    Directory of Open Access Journals (Sweden)

    Ade Muhammad

    2013-09-01

    Full Text Available The phenomenon of Shrinking Defense Capabilities is only the tip of the iceberg of Indonesian Defense System’s problem. The root of the problem that lies beneath and outside is keep untouched. This journal is the part of the efforts to reveal the phenomenon’s structure that binding as a system. The present structure is a cause of that phenomenon and the basis of the current organization responsible for Republic Indonesia Defense sector. With the Systems thinking analysis, the research has revealed the structure that become the problem cause and give a holistic solution trough redesigning new structure based on two theories namely the Strategic-Operational Management with Vision Dissemination of Prof. Dr. Jürgen Strohhecker and the Defense System Generic Dr. Ir. Muhammad Tasrif, M.Eng and Ir. Ade Muhammad, M.Han. The logical consequences would also changing of formation of Indonesian Defense Organization. Keywords : Design, Redesigning, Defense System, System, Defense, Holistic

  5. 77 FR 39125 - Defense Acquisition Regulations System; Defense Federal Acquisition Regulation Supplement; Only...

    Science.gov (United States)

    2012-06-29

    ... at 76 FR 44293 on July 25, 2011, to address acquisitions using competitive procedures in which only..., 2011 (76 FR 59623) through October 7, 2011. DoD received comments on the proposed rule from 19... Supplement; Only One Offer (DFARS Case 2011-D013); Defense Federal Acquisition Regulation...

  6. Testicular defense systems: immune privilege and innate immunity.

    Science.gov (United States)

    Zhao, Shutao; Zhu, Weiwei; Xue, Shepu; Han, Daishu

    2014-09-01

    The mammalian testis possesses a special immunological environment because of its properties of remarkable immune privilege and effective local innate immunity. Testicular immune privilege protects immunogenic germ cells from systemic immune attack, and local innate immunity is important in preventing testicular microbial infections. The breakdown of local testicular immune homeostasis may lead to orchitis, an etiological factor of male infertility. The mechanisms underlying testicular immune privilege have been investigated for a long time. Increasing evidence shows that both a local immunosuppressive milieu and systemic immune tolerance are involved in maintaining testicular immune privilege status. The mechanisms underlying testicular innate immunity are emerging based on the investigation of the pattern recognition receptor-mediated innate immune response in testicular cells. This review summarizes our current understanding of testicular defense mechanisms and identifies topics that merit further investigation. PMID:24954222

  7. Dynamic production scheduling in virtual cellular manufacturing systems

    OpenAIRE

    马俊; Ma, Jun

    2012-01-01

    Manufacturing companies must constantly improve productivity to respond to dynamic changes in customer demand in order to maintain their competitiveness and marketshares. This requires manufacturers to adopt more efficient methodologies to design and control their manufacturing systems. In recent decades, virtual cellular manufacturing (VCM), as an advanced manufacturing concept, has attracted increasing attention in the research community, because traditional cellular manufacturing is inadeq...

  8. Control Systems Cyber Security: Defense-in-Depth Strategies

    Energy Technology Data Exchange (ETDEWEB)

    Mark Fabro

    2007-10-01

    Information infrastructures across many public and private domains share several common attributes regarding IT deployments and data communications. This is particularly true in the control systems domain. A majority of the systems use robust architectures to enhance business and reduce costs by increasing the integration of external, business, and control system networks. However, multi-network integration strategies often lead to vulnerabilities that greatly reduce the security of an organization, and can expose mission-critical control systems to cyber threats. This document provides guidance and direction for developing ‘defense-in-depth’ strategies for organizations that use control system networks while maintaining a multi-tier information architecture that requires: • Maintenance of various field devices, telemetry collection, and/or industrial-level process systems • Access to facilities via remote data link or modem • Public facing services for customer or corporate operations • A robust business environment that requires connections among the control system domain, the external Internet, and other peer organizations.

  9. Challenges in Characterizing and Controlling Complex Cellular Systems

    Science.gov (United States)

    Wikswo, John

    2011-03-01

    Multicellular dynamic biological processes such as developmental differentiation, wound repair, disease, aging, and even homeostasis can be represented by trajectories through a phase space whose extent reflects the genetic, post-translational, and metabolic complexity of the process - easily extending to tens of thousands of dimensions. Intra- and inter-cellular sensing and regulatory systems and their nested, redundant, and non-linear feed-forward and feed-back controls create high-dimensioned attractors in this phase space. Metabolism provides free energy to drive non-equilibrium processes and dynamically reconfigure attractors. Studies of single molecules and cells provide only minimalist projections onto a small number of axes. It may be difficult to infer larger-scale emergent behavior from linearized experiments that perform only small amplitude perturbations on a limited number of the dimensions. Complete characterization may succeed for bounded component problems, such as an individual cell cycle or signaling cascade, but larger systems problems will require a coarse-grained approach. Hence a new experimental and analytical framework is needed. Possibly one could utilize high-amplitude, multi-variable driving of the system to infer coarse-grained, effective models, which in turn can be tested by their ability to control systems behavior. Navigation at will between attractors in a high-dimensioned dynamical system will provide not only detailed knowledge of the shape of attractor basins, but also measures of underlying stochastic events such as noise in gene expression or receptor binding and how both affect system stability and robustness. Needed for this are wide-bandwidth methods to sense and actuate large numbers of intracellular and extracellular variables and automatically and rapidly infer dynamic control models. The success of this approach may be determined by how broadly the sensors and actuators can span the full dimensionality of the phase space

  10. Spectral efficiency of cellular land mobile radio systems

    OpenAIRE

    Hammuda, H; McGeehan, JP; Bateman, A.

    1988-01-01

    A rigorous and comprehensive approach to the definition and evaluation of spectral efficiency of cellular land mobile radio systems is presented. The method accounts for all pertinent system parameters within a cellular land mobile radio network. Subjective tests to evaluate the protection ratio for various systems is thought to be imperative to the evaluation of spectral efficiency. A comparison is made of a number of current and proposed mobile radio schemes based on the spectral efficiency...

  11. Theater ballistic missile defense: modeling and analysis of the Marine Corps "HAWK" Missile Defense System

    OpenAIRE

    Monroe, William James Fredrick.

    1995-01-01

    This thesis develops the concept of integrating IDEF modeling with matrix analysis to explore the current state of the Marine Corps, 'As Is', Theater Ballistic Missile Defense information architecture. It demonstrates the possibility of using matrix analysis in conjunction with IDEF modeling to identify deficiencies within an existing information architecture. Using this framework, new technologies and advancements can also be measured to ensure they accurately address deficiencies identified...

  12. Cellular Particle Dynamics simulation of biomechanical relaxation processes of multi-cellular systems

    Science.gov (United States)

    McCune, Matthew; Kosztin, Ioan

    2013-03-01

    Cellular Particle Dynamics (CPD) is a theoretical-computational-experimental framework for describing and predicting the time evolution of biomechanical relaxation processes of multi-cellular systems, such as fusion, sorting and compression. In CPD, cells are modeled as an ensemble of cellular particles (CPs) that interact via short range contact interactions, characterized by an attractive (adhesive interaction) and a repulsive (excluded volume interaction) component. The time evolution of the spatial conformation of the multicellular system is determined by following the trajectories of all CPs through numerical integration of their equations of motion. Here we present CPD simulation results for the fusion of both spherical and cylindrical multi-cellular aggregates. First, we calibrate the relevant CPD model parameters for a given cell type by comparing the CPD simulation results for the fusion of two spherical aggregates to the corresponding experimental results. Next, CPD simulations are used to predict the time evolution of the fusion of cylindrical aggregates. The latter is relevant for the formation of tubular multi-cellular structures (i.e., primitive blood vessels) created by the novel bioprinting technology. Work supported by NSF [PHY-0957914]. Computer time provided by the University of Missouri Bioinformatics Consortium.

  13. Interference cancellation technique under imperfect synchronization in cellular systems

    Institute of Scientific and Technical Information of China (English)

    WANG; Xin; WU; Zhuo

    2009-01-01

    In this paper, an asynchronous cooperative cellular system applied with space-time block coding(STBC)is investigated. A signal detector is proposed based on parallel interference cancellation(PIC), to cancel the inter-symbol interference(ISI)caused by the imperfect synchronization. Simulation results show that the proposed PIC detector can effectively suppress the ISI, but there is still a comparatively high error floor, due to the co-channel interference(CCI)of the cellular system.

  14. Cellular transcriptional profiling in influenza A virus-infected lung epithelial cells: The role of the nonstructural NS1 protein in the evasion of the host innate defense and its potential contribution to pandemic influenza

    Science.gov (United States)

    Geiss, Gary K.; Salvatore, Mirella; Tumpey, Terrence M.; Carter, Victoria S.; Wang, Xiuyan; Basler, Christopher F.; Taubenberger, Jeffery K.; Bumgarner, Roger E.; Palese, Peter; Katze, Michael G.; García-Sastre, Adolfo

    2002-08-01

    The NS1 protein of influenza A virus contributes to viral pathogenesis, primarily by enabling the virus to disarm the host cell type IFN defense system. We examined the downstream effects of NS1 protein expression during influenza A virus infection on global cellular mRNA levels by measuring expression of over 13,000 cellular genes in response to infection with wild-type and mutant viruses in human lung epithelial cells. Influenza A/PR/8/34 virus infection resulted in a significant induction of genes involved in the IFN pathway. Deletion of the viral NS1 gene increased the number and magnitude of expression of cellular genes implicated in the IFN, NF-B, and other antiviral pathways. Interestingly, different IFN-induced genes showed different sensitivities to NS1-mediated inhibition of their expression. A recombinant virus with a C-terminal deletion in its NS1 gene induced an intermediate cellular mRNA expression pattern between wild-type and NS1 knockout viruses. Most significantly, a virus containing the 1918 pandemic NS1 gene was more efficient at blocking the expression of IFN-regulated genes than its parental influenza A/WSN/33 virus. Taken together, our results suggest that the cellular response to influenza A virus infection in human lung cells is significantly influenced by the sequence of the NS1 gene, demonstrating the importance of the NS1 protein in regulating the host cell response triggered by virus infection.

  15. Defense Nuclear Material Stewardship Integrated Inventory Information Management System (IIIMS)

    International Nuclear Information System (INIS)

    Sandia National Laboratories was tasked with developing the Defense Nuclear Material Stewardship Integrated Inventory Information Management System (IIIMS) with the sponsorship of NA-125.3 and the concurrence of DOE/NNSA field and area offices. The purpose of IIIMS was to modernize nuclear materials management information systems at the enterprise level. Projects over the course of several years attempted to spearhead this modernization. The scope of IIIMS was broken into broad enterprise-oriented materials management and materials forecasting. The IIIMS prototype was developed to allow multiple participating user groups to explore nuclear material requirements and needs in detail. The purpose of material forecasting was to determine nuclear material availability over a 10 to 15 year period in light of the dynamic nature of nuclear materials management. Formal DOE Directives (requirements) were needed to direct IIIMS efforts but were never issued and the project has been halted. When restarted, duplicating or re-engineering the activities from 1999 to 2003 is unnecessary, and in fact future initiatives can build on previous work. IIIMS requirements should be structured to provide high confidence that discrepancies are detected, and classified information is not divulged. Enterprise-wide materials management systems maintained by the military can be used as overall models to base IIIMS implementation concepts upon.

  16. Defense Nuclear Material Stewardship Integrated Inventory Information Management System (IIIMS).

    Energy Technology Data Exchange (ETDEWEB)

    Aas, Christopher A.; Lenhart, James E.; Bray, Olin H.; Witcher, Christina Jenkin

    2004-11-01

    Sandia National Laboratories was tasked with developing the Defense Nuclear Material Stewardship Integrated Inventory Information Management System (IIIMS) with the sponsorship of NA-125.3 and the concurrence of DOE/NNSA field and area offices. The purpose of IIIMS was to modernize nuclear materials management information systems at the enterprise level. Projects over the course of several years attempted to spearhead this modernization. The scope of IIIMS was broken into broad enterprise-oriented materials management and materials forecasting. The IIIMS prototype was developed to allow multiple participating user groups to explore nuclear material requirements and needs in detail. The purpose of material forecasting was to determine nuclear material availability over a 10 to 15 year period in light of the dynamic nature of nuclear materials management. Formal DOE Directives (requirements) were needed to direct IIIMS efforts but were never issued and the project has been halted. When restarted, duplicating or re-engineering the activities from 1999 to 2003 is unnecessary, and in fact future initiatives can build on previous work. IIIMS requirements should be structured to provide high confidence that discrepancies are detected, and classified information is not divulged. Enterprise-wide materials management systems maintained by the military can be used as overall models to base IIIMS implementation concepts upon.

  17. The brittleness model of complex system based on cellular automata

    Institute of Scientific and Technical Information of China (English)

    LIN De-ming; JIN Hong-zhang; LI Qi; WU Hong-mei

    2004-01-01

    Now the research on the complex system is a hot spot. Brittleness is one of the basic characteristics of a complex system. In a complex system, after one of subsystems is struck to be collapsed, the whole system will collapse. Meanwhile, cellular automata is a discrete dynamic system. When the rule is given, the cellular automata could be defined. Then it can imitate the complex action. Cellular automata is used to simulate the brittleness action in this study. Entropy was used to analyze the action and get the rule. Then,three normal brittleness models were given. The result shows that the brittleness of complex system is existent and in addition some important behavior mode of complex system brittleness has been achieved.

  18. A methodology for the derivation of survivability and reliability requirements for a Strategic Defense System

    OpenAIRE

    Lee, Wai H.

    1989-01-01

    Survivability and reliability have become important factors in the design of a mission effective Strategic Defense System (SDS) that protects the United States against a ballistic missile attack by the Soviet Union. The SDS must be reliable and responsive in providing this defense. It must also be survivable to counter the Soviet tactics of negating the defense. To ensure an effective, responsive and affordable system, survivability and reliability features must be incorporated...

  19. Defense Acquisition and Budgeting: Investigating the Adequacy of Linkage Between Systems

    OpenAIRE

    Jones, L. R.; McCaffery, Jerry L.

    2005-01-01

    In this article we assess evidence and test the hypothesis that the complicated architecture and processes of national defense planning, programming, budgeting and execution and the defense acquisition decision system produce system linkage weaknesses that lead to unintended and negative consequences for defense acquisition and procurement. The purpose of this article is to identify key points of linkage weakness and failure between DOD financial management and acquisition deci...

  20. Immunoisolation Patch System for Cellular Transplantation

    Science.gov (United States)

    Wang, Taylor G. (Inventor)

    2014-01-01

    An immunoisolation patch system, and particularly a patch system comprising multiple immunoisolation microcapsules, each encapsulating biological material such as cells for transplantation, which can be used in the prophylactic and therapeutic treatment of disease in large animals and humans without the need for immunosuppression.

  1. Performance evaluation of cellular layouts : extension to DRC system contexts

    NARCIS (Netherlands)

    Suresh, NC; Gaalman, GJC

    2000-01-01

    This study involves a comparison of the performance of functional layouts (FL) and cellular manufacturing (CM) systems in a dual-resource-constrained( DRC) system context. Past studies of FL and CM have been based mostly on single-resource-constrained( SRC) systems. Recent studies have included labo

  2. Interactions between Polymyxa betae and plant systemic defense ways

    OpenAIRE

    Desoignies, Nicolas; Schramme, Florence; Legrève, Anne; Vienna Interntational Plant Conference Association - Plant Diseases and resistance mechanisms

    2013-01-01

    Polymyxa betae is the vector of Beet necrotic yellow vein virus (BNYVV), the causal agent of sugar beet rhizomania disease. Because of the widespread use of cultivars partially resistant to BNYVV, resistance breaking BNYVV isolates have been reported. In order to develop alternative control strategies, we investigated interactions between P. betae and plant defenses. A first set of bioassays was conducted in order to assess P. betae infection after the elicitation of inducible defenses in sug...

  3. Piezomechatronic-based systems in aircraft, space, and defense applications

    Science.gov (United States)

    Maillard, T.; Claeyssen, F.; LeLetty, R.; Sosnicki, O.; Pages, A.; Vazquez Carazo, A.

    2009-05-01

    In Space & Defense fields, there is a trend for miniaturisation in active optics, fine instruments, robotic missions, microsatellites, UAVs, MAVs which directly impact on the design of actuators. A new generation of small and smart actuators such like piezoelectric (piezo) actuators, are responding to this trend, thanks to their capacity to offer high energy density and to support both extreme and various requirements. In Space vehicles, UAVs, missiles, military vehicles, etc., onboard place and available electric power can be very limited. For instance, a micro satellite often must operate all its instruments with less than 100W of power. As a result, allocated electric power per actuator is typically between 0.1 to 10W. This is also the case in small UAVs and in MAVs. Because of the high cost of embedded mass, space & military actuators need also to offer high output energy to mass ratio. One of the main difficulties is often the ability to withstand launching vibrations and shocks. Space environments add other constrains. A clear example is the vacuum conditions, which can induce difficulties to release the heat out off the actuator or for out gassing near optics. Other critical spacerelated environmental conditions include the thermal operation range required as well as the radiation-resistant requirements. In other situations, actuator strength to humidity is often an issue, especially for piezoelectric ceramics. Thus, the success of the application relies not only on design issues but also on material reliability. Specific actions at this level are needed to be undertaken to secure space projects. To cope with these issues and to illustrate the trend, the piezo actuators and mechanisms from Cedrat are presented. They have been initially developed and qualified to meet space requirements but logically found also applications in defense and micro aerial vehicle fields, for various micromechatronic functions. The paper presents typical applications and piezo

  4. Traffic Engineering of Cellular Wireless Communication Systems

    DEFF Research Database (Denmark)

    Iversen, Villy Bæk

    2002-01-01

    In mobile communications an efficient utilisation of the channels is of great importance. In this paper we describe the basic principles for obtaining the maximum utilisation and study strategies for obtaining these limits. In general a high degree of sharing is efficient, but requires service pr...... arrival processes, and multi-rate (multi-media) traffic for third generation systems....

  5. Cross-Layer Energy Optimization in Cooperative Cellular Systems

    Directory of Open Access Journals (Sweden)

    M.Sushanth Babu

    2012-10-01

    Full Text Available Cooperative networking is currently under standardization for future wireless cellular systems. This paper considers energy efficient transmission schemes in cooperative cellular transceiver systems. In which, we analyze how to minimize energy consumption per information bit in single link. Further, Target bit error probability, Packet length, Retransmission and Transmission distance for both coded and un coded system are considered as the performance metrics for the optimization of energy. The simulation results illustrate the effect of energy efficient modulation schemes for variable distances and fixed bit error probability.

  6. Modeling evolution and immune system by cellular automata

    Energy Technology Data Exchange (ETDEWEB)

    Bezzi, M. [Scuola Internazionale Superiore di Studi Avanzati, Trieste (Italy); Istituto Nazionale di Fisica della Materia, Florence (Italy)

    2001-07-01

    In this review the behavior of two different biological systems is investigated using cellular automata. Starting from this spatially extended approach it is also tried, in some cases, to reduce the complexity of the system introducing mean-field approximation, and solving (or trying to solve) these simplified systems. It is discussed the biological meaning of the results, the comparison with experimental data (if available) and the different features between spatially extended and mean-field versions. The biological systems considered in this review are the following: Darwinian evolution in simple ecosystems and immune system response. In the first section the main features of molecular evolution are introduced, giving a short survey of genetics for physicists and discussing some models for prebiotic systems and simple ecosystems. It is also introduced a cellular automaton model for studying a set of evolving individuals in a general fitness landscape, considering also the effects of co-evolution. In particular the process of species formation (speciation) is described in sect. 5. The second part deals with immune system modeling. The biological features of immune response are discussed, as well as it is introduced the concept of shape space and of idiotypic network. More detailed reviews which deal with immune system models (mainly focused on idiotypic network models) can be found. Other themes here discussed: the applications of CA to immune system modeling, two complex cellular automata for humoral and cellular immune response. Finally, it is discussed the biological data and the general conclusions are drawn in the last section.

  7. Modeling evolution and immune system by cellular automata

    International Nuclear Information System (INIS)

    In this review the behavior of two different biological systems is investigated using cellular automata. Starting from this spatially extended approach it is also tried, in some cases, to reduce the complexity of the system introducing mean-field approximation, and solving (or trying to solve) these simplified systems. It is discussed the biological meaning of the results, the comparison with experimental data (if available) and the different features between spatially extended and mean-field versions. The biological systems considered in this review are the following: Darwinian evolution in simple ecosystems and immune system response. In the first section the main features of molecular evolution are introduced, giving a short survey of genetics for physicists and discussing some models for prebiotic systems and simple ecosystems. It is also introduced a cellular automaton model for studying a set of evolving individuals in a general fitness landscape, considering also the effects of co-evolution. In particular the process of species formation (speciation) is described in sect. 5. The second part deals with immune system modeling. The biological features of immune response are discussed, as well as it is introduced the concept of shape space and of idiotypic network. More detailed reviews which deal with immune system models (mainly focused on idiotypic network models) can be found. Other themes here discussed: the applications of CA to immune system modeling, two complex cellular automata for humoral and cellular immune response. Finally, it is discussed the biological data and the general conclusions are drawn in the last section

  8. Evolution of Cellular Automata using Lindenmayer Systems and Fourier Transforms

    OpenAIRE

    Berg, Sivert

    2013-01-01

    Cellular automata (CAs) are a class of highly parallel computing systems consisting of many simple computing elements called cells. The cells can only communicate with neighboring cells, meaning there is no global communication in the system. Programming such a system to solve complex problems can be a daunting task, and indirect methods are often applied to make it easier. In this thesis we use evolutionary algorithms (EAs) to evolve CAs. We also look at the possibility of employing L-system...

  9. Hyper bio assembler for 3D cellular systems

    CERN Document Server

    Arai, Fumihito; Yamato, Masayuki

    2015-01-01

    Hyper Bio Assembler for Cellular Systems is the first book to present a new methodology for measuring and separating target cells at high speed and constructing 3D cellular systems in vitro. This book represents a valuable resource for biologists, biophysicists and robotic engineers, as well as researchers interested in this new frontier area, offering a better understanding of the measurement, separation, assembly, analysis and synthesis of complex biological tissue, and of the medical applications of these technologies. This book is the outcome of the new academic fields of the Ministry of Education, Culture, Sports, Science and Technology’s Grant-in-Aid for Scientific Research in Japan.

  10. Crystal structure of a murine α-class glutathione S-transferase involved in cellular defense against oxidative stress

    NARCIS (Netherlands)

    Krengel, Ute; Schröter, Klaus-Hasso; Hoier, Helga; Arkema, Anita; Kalk, Kor H.; Zimniak, Piotr; Dijkstra, Bauke W.

    1998-01-01

    Glutathione S-transferases (GSTs) are ubiquitous multifunctional enzymes which play a key role in cellular detoxification. The enzymes protect the cells against toxicants by conjugating them to glutathione. Recently, a novel subgroup of α-class GSTs has been identified with altered substrate specifi

  11. PROVIDING FINANCIAL SUPPORT FOR THE NATIONAL DEFENSE SYSTEM IN THE CURRENT GEOPOLITICAL CONTEXT

    OpenAIRE

    VASILE DINU

    2016-01-01

    This paper highlights the most important aspects, seen mainly from an economic and financial perspective, of the correlation between the needs of the national defense system with the possibilities conferred by the development stage of our country, considering that Romania is a NATO member, with certain commitments in this regard. Obviously, based on the recent Political Agreement on increasing national defense funding, we c...

  12. 75 FR 71560 - Defense Federal Acquisition Regulation Supplement; Cost and Software Data Reporting System (DFARS...

    Science.gov (United States)

    2010-11-24

    ... the cost and software data reporting requirements. DoD published a proposed rule at 75 FR 25165 on May... from small entities and other interested parties (proposed rule at 75 FR 25165). No comments were... Defense Acquisition Regulations System 48 CFR Parts 215, 234, 242, and 252 RIN 0750-AG46 Defense...

  13. Antioxidative defense

    Directory of Open Access Journals (Sweden)

    Stevanović Jelka

    2011-01-01

    Full Text Available Free radicals occur constantly during metabolism and take part in numerous physiological processes, such as: intra-cellular and inter-cellular signalization, gene expression, removal of damaged or senescent cells, and control of the tone of blood vessels. However, there is an increased quantity of free radicals in situations of so-called oxidative stress, when they cause serious damage to cellular membranes (peroxidation of their lipids, damage of membrane proteins, and similar, to interior cellular protein molecules, as well as DNA molecules and carbohydrates. This is precisely why the organism has developed numerous mechanisms for removing free radicals and/or preventing their production. Some of these are enzyme-related and include superoxide-dismutase, catalase, glutathione-peroxidase, and others. Other, non-enzyme mechanisms, imply antioxidative activities of vitamins E and C, provitamin A, coenzyme Q, reduced glutation, and others. Since free radicals can leave the cell that has produced them and become dispersed throughout the body, in addition to antioxidative defense that functions within cellular structures, antioxidant extra-cellular defense has also been developed. This is comprised by: transferrin, lactoferrin, haptoglobin, hemopexin, ceruloplasmin, albumins, extra-cellular isoform SOD, extracellular glutathione-peroxidase, glucose, bilirubin, urates, and many other molecules.

  14. Combined activation of the energy and cellular-defense pathways may explain the potent anti-senescence activity of methylene blue.

    Science.gov (United States)

    Atamna, Hani; Atamna, Wafa; Al-Eyd, Ghaith; Shanower, Gregory; Dhahbi, Joseph M

    2015-12-01

    Methylene blue (MB) delays cellular senescence, induces complex-IV, and activates Keap1/Nrf2; however, the molecular link of these effects to MB is unclear. Since MB is redox-active, we investigated its effect on the NAD/NADH ratio in IMR90 cells. The transient increase in NAD/NADH observed in MB-treated cells triggered an investigation of the energy regulator AMPK. MB induced AMPK phosphorylation in a transient pattern, which was followed by the induction of PGC1α and SURF1: both are inducers of mitochondrial and complex-IV biogenesis. Subsequently MB-treated cells exhibited >100% increase in complex-IV activity and a 28% decline in cellular oxidants. The telomeres erosion rate was also significantly lower in MB-treated cells. A previous research suggested that the pattern of AMPK activation (i.e., chronic or transient) determines the AMPK effect on cell senescence. We identified that the anti-senescence activity of MB (transient activator) was 8-times higher than that of AICAR (chronic activator). Since MB lacked an effect on cell cycle, an MB-dependent change to cell cycle is unlikely to contribute to the anti-senescence activity. The current findings in conjunction with the activation of Keap1/Nrf2 suggest a synchronized activation of the energy and cellular defense pathways as a possible key factor in MB's potent anti-senescence activity. PMID:26386875

  15. Towards a continuum theory of movement in interacting cellular systems

    Science.gov (United States)

    Newman, Timothy

    2003-10-01

    Interacting cellular systems form the basis of all higher organisms, and are fundamental to the understanding of embryogenesis, organ function, and neoplasms. I will describe a stochastic model of cell interactions which can be applied to these problems, and present some of our recent results on chemotactic response.

  16. [Oxidative injury and its defense system in vivo].

    Science.gov (United States)

    Niwa, Y

    1999-03-01

    We and other researchers verified that excessively produced free radicals by neutrophils induce various diseases such as Behçet's disease, MCLS, SLE (neutrophil-stimulated lymphocytes), RA (synovial fluid neutrophils), Crohn's disease, colitis ulcerosa, and dermatitis herpetiformis (Dühring). Recently, it was reported that environmental toxic agents including herbicides such as paraquat, insecticides, nitrogen oxide, and ultraviolet radiation produce free radicals. Nitrogen oxide, a main product of the combustion of petroleum, is a prominent component of exhaust from automobiles. Generation of 1O2 by ultraviolet radiation has also increased by breaks in the earth's ozone layer induced by halogenated hydrocarbon gas. Various diseases have been induced by these agents such as malignancies, severe adult atopic dermatitis, complication of cataract and retinolysis with atopic dermatitis, skin cancer, male infertility, severe collagen diseases, and lung fibrosis. It was also found that PCB, methyl-Hg and Mn, Cd induce neuropathic diseases through the increase in free radical production. On the other hand, a self-defense system exists against oxidative injuries; high-molecular-weight antioxidants such as SOD, catalase, and GSH-Px, and low-molecular-weight antioxidants such as vitamin C, E, A, polyphenols, flavonoids, and catechin. As protection from oxidative injury, various antioxidant products have been developed, however, the development of SOD injection was given up by all the pharmaceutical companies in the world on account of ineffectiveness due to rapid excretion from the kidney, low affinity to the receptor and weak penetration into the cell. A.M. Michelson, a French biochemist succeeded in developing an effective bovine liposomal-encapsulated SOD solving these problems, however, he also gave it up since French government prohibited bovine products due to the prion virus. Regarding low-molecular-weight antioxidants, synthetic products generally show low affinity

  17. The Tumorigenic Roles of the Cellular REDOX Regulatory Systems

    OpenAIRE

    Stéphanie Anaís Castaldo; Joana Raquel Freitas; Nadine Vasconcelos Conchinha; Patrícia Alexandra Madureira

    2016-01-01

    The cellular REDOX regulatory systems play a central role in maintaining REDOX homeostasis that is crucial for cell integrity, survival, and proliferation. To date, a substantial amount of data has demonstrated that cancer cells typically undergo increasing oxidative stress as the tumor develops, upregulating these important antioxidant systems in order to survive, proliferate, and metastasize under these extreme oxidative stress conditions. Since a large number of chemotherapeutic agents cur...

  18. Proteomic characterization of an isolated fraction of synthetic proteasome inhibitor (PSI-induced inclusions in PC12 cells might offer clues to aggresomes as a cellular defensive response against proteasome inhibition by PSI

    Directory of Open Access Journals (Sweden)

    Li Xing'an

    2010-08-01

    Full Text Available Abstract Background Cooperation of constituents of the ubiquitin proteasome system (UPS with chaperone proteins in degrading proteins mediate a wide range of cellular processes, such as synaptic function and neurotransmission, gene transcription, protein trafficking, mitochondrial function and metabolism, antioxidant defence mechanisms, and apoptotic signal transduction. It is supposed that constituents of the UPS and chaperone proteins are recruited into aggresomes where aberrant and potentially cytotoxic proteins may be sequestered in an inactive form. Results To determinate the proteomic pattern of synthetic proteasome inhibitor (PSI-induced inclusions in PC12 cells after proteasome inhibition by PSI, we analyzed a fraction of PSI-induced inclusions. A proteomic feature of the isolated fraction was characterized by identification of fifty six proteins including twenty previously reported protein components of Lewy bodies, twenty eight newly identified proteins and eight unknown proteins. These proteins, most of which were recognized as a profile of proteins within cellular processes mediated by the UPS, a profile of constituents of the UPS and a profile of chaperone proteins, are classed into at least nine accepted categories. In addition, prolyl-4-hydroxylase beta polypeptide, an endoplasmic reticulum member of the protein disulfide isomerase family, was validated in the developmental process of PSI-induced inclusions in the cells. Conclusions It is speculated that proteomic characterization of an isolated fraction of PSI-induced inclusions in PC12 cells might offer clues to appearance of aggresomes serving as a cellular defensive response against proteasome inhibition.

  19. Uplink Channel Dependent Scheduling for Future Cellular Systems

    OpenAIRE

    Jersenius, Kristina

    2007-01-01

    One goal in the development of future cellular systems is to increase performance. Channel dependent scheduling can possibly contribute to a performance enhancement. It requires channel qualityinformation and uplink channel knowledge is often incomplete. This master thesis work compares channel dependent scheduling and channel independent scheduling for a Single Carrier Frequency Division Multiple Access-based uplink in time domain and time and frequencydomain assuming continuous channel qual...

  20. Evaluating Defense Architecture Frameworks for C4I System Using Analytic Hierarchy Process

    Directory of Open Access Journals (Sweden)

    Abdullah S. Alghamdi

    2009-01-01

    Full Text Available Problem statement: The Command, Control, Communications, Computers and Intelligence (C4I Systems provided situational awareness about operational environment and supported in decision making and directed to operative environment. These systems had been used by various agencies like defense, police, investigation, road, rail, airports, oil and gas related department. However, the increase use of C4I system had made it more important and attractive. Consequently interest in design and development of C4I system had increased among the researchers. Many defense industry frameworks were available but the problem was a suitable selection of a framework in design and development of C4I system. Approach: This study described the concepts, tool and methodology being used for evaluation analysis of different frameworks by Analytic Hierarchy Process (AHP. Results: We had compared different defense industry frameworks like Department of Defense Architecture Framework (DODAF, Ministry of Defense Architecture Framework (MODAF and NATO Architecture Framework (NAF and found that AHP is fairly good tool in terms of analysis. Conclusion: Different defense industry frameworks such as DODAF, MODAF and NAF had been evaluated and compared using AHP.

  1. Induction of multixenobiotic defense mechanisms in resistant Daphnia magna clones as a general cellular response to stress.

    Science.gov (United States)

    Jordão, Rita; Campos, Bruno; Lemos, Marco F L; Soares, Amadeu M V M; Tauler, Romà; Barata, Carlos

    2016-06-01

    Multixenobiotic resistance mechanisms (MXR) were recently identified in Daphnia magna. Previous results characterized gene transcripts of genes encoding and efflux activities of four putative ABCB1 and ABCC transporters that were chemically induced but showed low specificity against model transporter substrates and inhibitors, thus preventing us from distinguishing between activities of different efflux transporter types. In this study we report on the specificity of induction of ABC transporters and of the stress protein hsp70 in clones selected to be genetically resistant to ABCB1 chemical substrates. Clones resistant to mitoxantrone, ivermectin and pentachlorophenol showed distinctive transcriptional responses of transporter protein coding genes and of putative transporter dye activities. Expression of hsp70 proteins also varied across resistant clones. Clones resistant to mitoxantrone and pentachlorophenol showed high constitutive levels of hsp70. Transcriptional levels of the abcb1 gene transporter and of putative dye transporter activity were also induced to a greater extent in the pentachlorophenol resistant clone. Observed higher dye transporter activities in individuals from clones resistant to mitoxantrone and ivermectin were unrelated with transcriptional levels of the studied four abcc and abcb1 transporter genes. These findings suggest that Abcb1 induction in D. magna may be a part of a general cellular stress response. PMID:27039215

  2. 48 CFR 47.301-3 - Using the Defense Transportation System (DTS).

    Science.gov (United States)

    2010-10-01

    ... 48 Federal Acquisition Regulations System 1 2010-10-01 2010-10-01 false Using the Defense Transportation System (DTS). 47.301-3 Section 47.301-3 Federal Acquisition Regulations System FEDERAL ACQUISITION... Transportation System (DTS). (a) All military and civilian agencies shipping, or arranging for the...

  3. Cellular structure of detonation utilized in propulsion system

    Science.gov (United States)

    Zhang, XuDong; Fan, BaoChun; Gui, MingYue; Pan, ZhenHua

    2012-10-01

    How to confine a detonation in a combustor is a key issue of detonation applications in propulsion systems. Based on achieving schemes, detonations applied in the combustor, including pulse detonation wave (PDW), oblique detonation wave (ODW) and rotating detonation wave (RDW), are different from that described by the classic CJ theory in fine structures and its self-sustaining mechanisms. In this work, the cellular structures and flow fields of ODW and RDW were obtained numerically, and the fundamental characteristics and self-sustaining mechanisms of the detonations were analyzed and discussed. ODW front consists of three parts: the ZND-like front, the single-headed triple point front and the dual-headed triple point front. Cellular structures of RDW are heterogeneous, and the cell size near the outer wall is smaller than that near the inner wall.

  4. Multiuser Scheduling on the Downlink of an LTE Cellular System

    Directory of Open Access Journals (Sweden)

    Raymond Kwan

    2008-01-01

    Full Text Available The challenge of scheduling user transmissions on the downlink of a long-term evolution (LTE cellular communication system is addressed. In particular, a novel optimalmultiuser scheduler is proposed. Numerical results show that the system performance improves with increasing correlation among OFDMA subcarriers. It is found that only a limited amount of feedback information is needed to achieve relatively good performance. A suboptimal reduced-complexity scheduler is also proposed and shown to provide good performance. The suboptimal scheme is especially attractive when the number of users is large, in which case the complexity of the optimal scheme is high.

  5. A novel mathematical model describing adaptive cellular drug metabolism and toxicity in the chemoimmune system.

    Directory of Open Access Journals (Sweden)

    Attila Tóth

    Full Text Available Cells cope with the threat of xenobiotic stress by activating a complex molecular network that recognizes and eliminates chemically diverse toxic compounds. This "chemoimmune system" consists of cellular Phase I and Phase II metabolic enzymes, Phase 0 and Phase III ATP Binding Cassette (ABC membrane transporters, and nuclear receptors regulating these components. In order to provide a systems biology characterization of the chemoimmune network, we designed a reaction kinetic model based on differential equations describing Phase 0-III participants and regulatory elements, and characterized cellular fitness to evaluate toxicity. In spite of the simplifications, the model recapitulates changes associated with acquired drug resistance and allows toxicity predictions under variable protein expression and xenobiotic exposure conditions. Our simulations suggest that multidrug ABC transporters at Phase 0 significantly facilitate the defense function of successive network members by lowering intracellular drug concentrations. The model was extended with a novel toxicity framework which opened the possibility of performing in silico cytotoxicity assays. The alterations of the in silico cytotoxicity curves show good agreement with in vitro cell killing experiments. The behavior of the simplified kinetic model suggests that it can serve as a basis for more complex models to efficiently predict xenobiotic and drug metabolism for human medical applications.

  6. A two-level formal specification of a defense communications system

    Energy Technology Data Exchange (ETDEWEB)

    Chisholm, G.H. [Argonne National Lab., IL (United States). Decision and Information Sciences Div.; Kemmerer, R.A. [Univ. of California, Santa Barbara, CA (United States). Reliable Software Group

    1994-04-01

    Computer systems are being used in critical situations with sensitive data, which makes it very important to ensure that these systems perform as desired. The defense communications system contains particularly sensitive data. A two-level ASLAN formal specification of a defense communications system is presented. The ASLAN model is designed to enhance the understanding of critical requirements and demands of the defense communications system. For the top-level (high-level) specifications, the structural details of the actual network are actual network are abstracted to allow more time for examining the interactions between the sites and the network. At this level, DataGrams move through the network, although the actual routing decisions are not specified. More details are added in the second-level specification. At this level, structure is added to the network.

  7. A Mean Field Game Approach to Scheduling in Cellular Systems

    OpenAIRE

    Manjrekar, Mayank; Ramaswamy, Vinod; Shakkottai, Srinivas

    2013-01-01

    We study auction-theoretic scheduling in cellular networks using the idea of mean field equilibrium (MFE). Here, agents model their opponents through a distribution over their action spaces and play the best response. The system is at an MFE if this action is itself a sample drawn from the assumed distribution. In our setting, the agents are smart phone apps that generate service requests, experience waiting costs, and bid for service from base stations. We show that if we conduct a second-pr...

  8. Optimal channel utilization and service protection in cellular communication systems

    DEFF Research Database (Denmark)

    Iversen, Villy Bæk

    In mobile communications an efficient utilization of the channels is of great importance.In this paper we consider the basic principles for obtaining the maximum utilization, and we study strategies for obtaining these limits.In general a high degree of sharing is efficient, but requires service...... protection mechanisms for protecting services and subscriber groups.We study cellular systems with overlaid cells, and the effect of overlapping cells, and we show that by dynamic channel allocation we obtain a high utilization.The models are generalizations of the Erlang-B formula, and can be evaluated...

  9. Modeling and simulation for train control system using cellular automata

    Institute of Scientific and Technical Information of China (English)

    LI; KePing; GAO; ZiYou; YANG; LiXing

    2007-01-01

    Train control system plays a key role in railway traffic. Its function is to manage and control the train movement on railway networks. In our previous works, based on the cellular automata (CA) model, we proposed several models and algorithms for simulating the train movement under different control system conditions. However, these models are only suitable for some simple traffic conditions. Some basic factors, which are important for train movement, are not considered. In this paper, we extend these models and algorithms and give a unified formula. Using the proposed method, we analyze and discuss the space-time diagram of railway traffic flow and the trajectories of the train movement. The numerical simulation and analytical results demonstrate that the unified CA model is an effective tool for simulating the train control system.

  10. Network Randomization and Dynamic Defense for Critical Infrastructure Systems

    Energy Technology Data Exchange (ETDEWEB)

    Chavez, Adrian R. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Martin, Mitchell Tyler [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Hamlet, Jason [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Stout, William M.S. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Lee, Erik [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-04-01

    Critical Infrastructure control systems continue to foster predictable communication paths, static configurations, and unpatched systems that allow easy access to our nation's most critical assets. This makes them attractive targets for cyber intrusion. We seek to address these attack vectors by automatically randomizing network settings, randomizing applications on the end devices themselves, and dynamically defending these systems against active attacks. Applying these protective measures will convert control systems into moving targets that proactively defend themselves against attack. Sandia National Laboratories has led this effort by gathering operational and technical requirements from Tennessee Valley Authority (TVA) and performing research and development to create a proof-of-concept solution. Our proof-of-concept has been tested in a laboratory environment with over 300 nodes. The vision of this project is to enhance control system security by converting existing control systems into moving targets and building these security measures into future systems while meeting the unique constraints that control systems face.

  11. The Dwight D. Eisenhower National System of Interstate and Defense Highways: The Road to Success?

    Science.gov (United States)

    Blas, Elisheva

    2010-01-01

    The Dwight D. Eisenhower National System of Interstate and Defense Highways was an innovation that changed America. The highway system, the greatest public works project in American history, made travel faster, easier, and safer. However, there were serious negative effects of the highways; they hindered the growth of cities, destroyed…

  12. Coarse-grained cellular automaton for traffic systems

    CERN Document Server

    Krawczyk, Malgorzata J

    2012-01-01

    A coarse-grained cellular automaton is proposed to simulate traffic systems. There, cells represent road sections. A cell can be in two states: jammed or passable. Numerical calculations are performed for a piece of square lattice with open boundary conditions, for the same piece with some cells removed and for a map of a small city. The results indicate the presence of a phase transition in the parameter space, between two macroscopic phases: passable and jammed. The results are supplemented by exact calculations of the stationary probabilities of states for the related Kripke structure constructed for the traffic system. There, the symmetry-based reduction of the state space allows to partially reduce the computational limitations of the numerical method.

  13. A MULTI CRITERIA APPROACH TO DESIGNING THE CELLULAR MANUFACTURING SYSTEM

    Directory of Open Access Journals (Sweden)

    Rika Ampuh Hadiguna

    2005-01-01

    Full Text Available Cellular manufacturing system design problems such as design framework, manufacturing cells layout and layout evaluation. The research objective is developing the framework to designing manufacturing cells with considering the organization and management aspects in shopfloor. In this research have compared the existing layout with proposed layout which applied the multi criteria approach. The proposed method is combining Analytical Hierarchy Process (AHP, Clustering and heuristic approach. The result has show that grouping with Single Linkage Clustering (SLC to be selected as manufacturing cells. The comparison of clustering weight is 0,567, 0,245 and 0,188 for SLC, Complete Linkage Clustering (CLC and Average Linkage Clustering (ALC, respectively. This result shows that generating layout by using grouping result from SLC. The evaluation result shows that types of manufacturing cells better than process layout which used the existing system.

  14. The coagulation system and its function in early immune defense.

    OpenAIRE

    T. van der Poll; Herwald, Heiko

    2014-01-01

    Blood coagulation has a Janus-faced role in infectious diseases. When systemically activated, it can cause serious complications associated with high morbidity and mortality. However, coagulation is also part of the innate immune system and its local activation has been found to play an important role in the early host response to infection. Though the latter aspect has been less investigated, phylogenetic studies have shown that many factors involved in coagulation have ancestral origins whi...

  15. Performance Analysis of Handoff in CDMA Cellular System

    Directory of Open Access Journals (Sweden)

    Dr. Dalveer Kaur

    2013-07-01

    Full Text Available Handoff mechanism is extremely important in cellular network because of the cellular architecture employed to maximize spectrum utilization. This unique feature has driven the rapid growth in the mobile network industry, changing it from a new technology into a massive industry within less than two decades. Handoff is the essential functionality for dealing with the mobility of the mobile users. This paper shows the soft handoff effects on the uplink direction of IS-95 CDMA networks is carried out, leading to optimize soft handoff for capacity under perfect power control approach. In practical systems, there is a nonzero handoff completion delay and soft handoff provides the required robustness to delays, although it comes at the expense of additional network resources. Thus, there is a tradeoff between the extent of soft handoff required and the handoff execution delay. This paper presents an analytical framework to study this tradeoff and also discuss simulation results simulated with the help of Matlab. For this, handoff dropping probability is minimized up to 0.1%.

  16. Vulnerability And Risk Assessment Using The Homeland-Defense Operational Planning System (HOPS)

    International Nuclear Information System (INIS)

    For over ten years, the Counterproliferation Analysis and Planning System (CAPS) at Lawrence Livermore National Laboratory (LLNL) has been a planning tool used by U.S. combatant commands for mission support planning against foreign programs engaged in the manufacture of weapons of mass destruction (WMD). CAPS is endorsed by the Secretary of Defense as the preferred counterproliferation tool to be used by the nation's armed services. A sister system, the Homeland-Defense Operational Planning System (HOPS), is a new operational planning tool leveraging CAPS expertise designed to support the defense of the U.S. homeland. HOPS provides planners with a basis to make decisions to protect against acts of terrorism, focusing on the defense of facilities critical to U.S. infrastructure. Criticality of facilities, structures, and systems is evaluated on a composite matrix of specific projected casualty, economic, and sociopolitical impact bins. Based on these criteria, significant unidentified vulnerabilities are identified and secured. To provide insight into potential successes by malevolent actors, HOPS analysts strive to base their efforts mainly on unclassified open-source data. However, more cooperation is needed between HOPS analysts and facility representatives to provide an advantage to those whose task is to defend these facilities. Evaluated facilities include: refineries, major ports, nuclear power plants and other nuclear licensees, dams, government installations, convention centers, sports stadiums, tourist venues, and public and freight transportation systems. A generalized summary of analyses of U.S. infrastructure facilities is presented

  17. Risk Assessment Using The Homeland-Defense Operational Planning System (HOPS)

    International Nuclear Information System (INIS)

    For over ten years, the Counterproliferation Analysis and Planning System (CAPS) at Lawrence Livermore National Laboratory (LLNL) has been a planning tool used by U.S. combatant commands for mission support planning against foreign programs engaged in the manufacture of weapons of mass destruction (WMD). CAPS is endorsed by the Secretary of Defense as the preferred counterproliferation tool to be used by the nation's armed services. A sister system, the Homeland-Defense Operational Planning System (HOPS), is a new operational planning tool leveraging CAPS expertise designed to support the defense of the U.S. homeland. HOPS provides planners with a basis to make decisions to protect against acts of terrorism, focusing on the defense of facilities critical to U.S. infrastructure. Criticality of facilities, structures, and systems is evaluated on a composite matrix of specific projected casualty, economic, and sociopolitical impact bins. Based on these criteria, significant unidentified vulnerabilities are identified and secured. To provide insight into potential successes by malevolent actors, HOPS analysts strive to base their efforts mainly on unclassified open-source data. However, more cooperation is needed between HOPS analysts and facility representatives to provide an advantage to those whose task is to defend these facilities. Evaluated facilities include: refineries, major ports, nuclear power plants and other nuclear licensees, dams, government installations, convention centers, sports stadiums, tourist venues, and public and freight transportation systems. A generalized summary of analyses of U.S. infrastructure facilities will be presented

  18. Risk Assessment Using The Homeland-Defense Operational Planning System (HOPS)

    Energy Technology Data Exchange (ETDEWEB)

    Durling, R L; Price, D E; Spero, K K

    2005-06-06

    For over ten years, the Counterproliferation Analysis and Planning System (CAPS) at Lawrence Livermore National Laboratory (LLNL) has been a planning tool used by U.S. combatant commands for mission support planning against foreign programs engaged in the manufacture of weapons of mass destruction (WMD). CAPS is endorsed by the Secretary of Defense as the preferred counterproliferation tool to be used by the nation's armed services. A sister system, the Homeland-Defense Operational Planning System (HOPS), is a new operational planning tool leveraging CAPS expertise designed to support the defense of the U.S. homeland. HOPS provides planners with a basis to make decisions to protect against acts of terrorism, focusing on the defense of facilities critical to U.S. infrastructure. Criticality of facilities, structures, and systems is evaluated on a composite matrix of specific projected casualty, economic, and sociopolitical impact bins. Based on these criteria, significant unidentified vulnerabilities are identified and secured. To provide insight into potential successes by malevolent actors, HOPS analysts strive to base their efforts mainly on unclassified open-source data. However, more cooperation is needed between HOPS analysts and facility representatives to provide an advantage to those whose task is to defend these facilities. Evaluated facilities include: refineries, major ports, nuclear power plants and other nuclear licensees, dams, government installations, convention centers, sports stadiums, tourist venues, and public and freight transportation systems. A generalized summary of analyses of U.S. infrastructure facilities will be presented.

  19. Vulnerability And Risk Assessment Using The Homeland-Defense Operational Planning System (HOPS)

    Energy Technology Data Exchange (ETDEWEB)

    Durling, Jr., R L; Price, D E; Spero, K K

    2005-01-03

    For over ten years, the Counterproliferation Analysis and Planning System (CAPS) at Lawrence Livermore National Laboratory (LLNL) has been a planning tool used by U.S. combatant commands for mission support planning against foreign programs engaged in the manufacture of weapons of mass destruction (WMD). CAPS is endorsed by the Secretary of Defense as the preferred counterproliferation tool to be used by the nation's armed services. A sister system, the Homeland-Defense Operational Planning System (HOPS), is a new operational planning tool leveraging CAPS expertise designed to support the defense of the U.S. homeland. HOPS provides planners with a basis to make decisions to protect against acts of terrorism, focusing on the defense of facilities critical to U.S. infrastructure. Criticality of facilities, structures, and systems is evaluated on a composite matrix of specific projected casualty, economic, and sociopolitical impact bins. Based on these criteria, significant unidentified vulnerabilities are identified and secured. To provide insight into potential successes by malevolent actors, HOPS analysts strive to base their efforts mainly on unclassified open-source data. However, more cooperation is needed between HOPS analysts and facility representatives to provide an advantage to those whose task is to defend these facilities. Evaluated facilities include: refineries, major ports, nuclear power plants and other nuclear licensees, dams, government installations, convention centers, sports stadiums, tourist venues, and public and freight transportation systems. A generalized summary of analyses of U.S. infrastructure facilities is presented.

  20. Changes in the glucosinolate-myrosinase defense system in Brassica juncea cotyledons during seedling development.

    Science.gov (United States)

    Wallace, S K; Eigenbrode, Sanford D

    2002-02-01

    Optimal defense theory (ODT) predicts that plant defenses will be allocated to plant organs and tissues in proportion to their relative fitness values and susceptibilities to attack. This study was designed to test ODT predictions on the myrosinase-glucosinolate defense system in Brassica juncea by examining the relationships between the fitness value of B. juncea cotyledons and the levels and effectiveness of cotyledon defenses. Specifically, we estimated fitness value of cotyledons during plant development by measuring plant growth and seed production after cotyledon damage or removal at successive seedling ages. Cotyledon removal within five days of emergence had a significant impact on growth and seed production, but cotyledon removal at later stages did not. Consistent with ODT, glucosinolate and myrosinase levels in cotyledons also declined with seedling age, as did relative defenses against a generalist herbivore, Spodoptera eridania, as estimated by bioassay. Declines in glucosinolates were as predicted by a passive, allometric dilution model based on cotyledon expansion. Declines in myrosinase activity were significantly more gradual than predicted by allometric dilution, suggesting active retention of myrosinase activity as young cotyledons expand. PMID:11925065

  1. Testicular defense systems: immune privilege and innate immunity

    OpenAIRE

    Zhao, Shutao; Zhu, Weiwei; Xue, Shepu; Han, Daishu

    2014-01-01

    The mammalian testis possesses a special immunological environment because of its properties of remarkable immune privilege and effective local innate immunity. Testicular immune privilege protects immunogenic germ cells from systemic immune attack, and local innate immunity is important in preventing testicular microbial infections. The breakdown of local testicular immune homeostasis may lead to orchitis, an etiological factor of male infertility. The mechanisms underlying testicular immune...

  2. Wireless Secrecy in Cellular Systems with Infrastructure--Aided Cooperation

    CERN Document Server

    Popovski, Petar

    2008-01-01

    In cellular systems, confidentiality of uplink transmission with respect to eavesdropping terminals can be ensured by creating intentional inteference via scheduling of concurrent downlink transmissions. In this paper, this basic idea is explored from an information-theoretic standpoint by focusing on a two-cell scenario where the involved base stations are connected via a finite-capacity backbone link. A number of transmission strategies are considered that aim at improving uplink confidentiality under constraints on the downlink rate that acts as an interfering signal. The strategies differ mainly in the way the backbone link is exploited by the cooperating downlink- to the uplink-operated base stations. Achievable rates are derived for both the Gaussian (unfaded) and the fading cases, under different assumptions on the channel state information available at different nodes. Numerical results are also provided to corroborate the analysis. Overall, the analysis reveals that a combination of scheduling and ba...

  3. Cellular Manufacturing System with Dynamic Lot Size Material Handling

    Science.gov (United States)

    Khannan, M. S. A.; Maruf, A.; Wangsaputra, R.; Sutrisno, S.; Wibawa, T.

    2016-02-01

    Material Handling take as important role in Cellular Manufacturing System (CMS) design. In several study at CMS design material handling was assumed per pieces or with constant lot size. In real industrial practice, lot size may change during rolling period to cope with demand changes. This study develops CMS Model with Dynamic Lot Size Material Handling. Integer Linear Programming is used to solve the problem. Objective function of this model is minimizing total expected cost consisting machinery depreciation cost, operating costs, inter-cell material handling cost, intra-cell material handling cost, machine relocation costs, setup costs, and production planning cost. This model determines optimum cell formation and optimum lot size. Numerical examples are elaborated in the paper to ilustrate the characterictic of the model.

  4. Summary note on report TR-61-B: EMP protective systems by the Defense Civil Preparedness Agency

    International Nuclear Information System (INIS)

    The EMP Protective Systems report presents a description of representative problems and solutions associated with providing protection against nuclear electromagnetic pulse for communication facilities. The protection techniques shown for the Emergency Operating Centers (usually small radio and telephone communication facilities) of the Defense Civil Preparedness Agency are applicable to many facilities. Numerous photos and diagrams augment the information in the text

  5. Second Line of Defense Virtual Private Network Guidance for Deployed and New CAS Systems

    Energy Technology Data Exchange (ETDEWEB)

    Singh, Surya V.; Thronas, Aaron I.

    2010-01-01

    This paper discusses the importance of remote access via virtual private network (VPN) for the Second Line of Defense (SLD) Central Alarm System (CAS) sites, the requirements for maintaining secure channels while using VPN and implementation requirements for current and future sites.

  6. Cellular mechanisms underlying the interaction between cannabinoid and opioid system.

    Science.gov (United States)

    Parolaro, D; Rubino, T; Viganò, D; Massi, P; Guidali, C; Realini, N

    2010-04-01

    Recently, the presence of functional interaction between the opioid and cannabinoid system has been shown in various pharmacological responses. Although there is an increasing interest for the feasible therapeutic application of a co-administration of cannabinoids and opioids in some disorders (i.e. to manage pain, to modulate immune system and emotions) and the combined use of the two drugs by drug abusers is becoming largely diffuse, only few papers focused on cellular and molecular mechanisms underlying this interaction. This review updates the biochemical and molecular underpinnings of opioid and cannabinoid interaction, both within the central nervous system and periphery. The most convincing theory for the explanation of this reciprocal interaction involves (i) the release of opioid peptides by cannabinoids or endocannabinoids by opioids, (ii) the existence of a direct receptor-receptor interaction when the receptors are co-expressed in the same cells, and (iii) the interaction of their intracellular pathways. Finally, the cannabinoid/opioid interaction might be different in the brain rewarding networks and in those accounting for other pharmacological effects (antinociception, modulation of emotionality and cognitive behavior), as well as between the central nervous system and periphery. Further insights about the cannabinoid/opioid interaction could pave the way for new and promising therapeutic approaches. PMID:20017730

  7. Ginsan activated the antioxidant defense systems in irradiated mice

    International Nuclear Information System (INIS)

    Ginsan, a polysaccharide extracted from Panax ginseng, has hematopoietic activity and is also known as a good biological-response modifier. In this investigation, we studied the effects of ginsan on the γ-radiation induced alterations of some antioxidant systems in spleen of Balb/c mice. There are many data that irradiation induces Reactive Oxygen Species (ROS), which plays an important causative role in radiation damage of cell. The level of ROS in cells is regulated by enzymatic and nonenzymatic antioxidant systems. The most powerful ones among them are superoxide dismutases (SODs) catalyzing the dismutation of superoxide anion radical o2 to H2O2, catalase deactivating h-2O2 and reduced glutathion (GSH) detoxifying H2O2 and other ROS> At the 5th day after sublethal whole body irradiation, splenocytes of irradiated mice expressed only marginally increased levels of Mn-SOD, however, Cu/Zn-SOD, catalase, thioredoxine reductase (TR) and thioredoxine (TRX) mRNA (135% increase compared to control), however, the combination of irradiation with ginsan increased the SODs and GPX production more effectively. In addition to the above results, we obtained the similar data of protein expression. The enzyme activities of SOD, catalase, and GPX of ginsan-treated and irradiated mice were significantly enhanced by 140, 115, 126% respectively, compared with those of irradiated mice. Based on these results, we propose that the induction of antioxidant enzymes of ginsan is at least in part due to its capacity to protect against radiation

  8. Y-12 defense programs. Nuclear Packaging Systems testing capabilities

    International Nuclear Information System (INIS)

    The Nuclear Packaging Systems (NPS) Department can manage/accomplish any packaging task. The NPS organization is responsible for managing the design, testing, certification, procurement, operation, refurbishment, maintenance, and disposal of packaging used to transport radioactive materials, other hazardous materials, and general cargoes on public roads and within the Oak Ridge Y-12 Plant. Additionally, the NPS Department has developed a Quality Assurance plan for all packaging, design and procurement of nonweapon shipping containers for radioactive materials, and design and procurement of performance-oriented packaging for hazardous materials. Further, the NPS Department is responsible for preparation and submittal of Safety Analysis Reports for Packaging (SARP). The NPS Department coordinates shipping container procurement and safety certification activities that have lead-times of up to two years. A Packaging Testing Capabilities Table at the Oak Ridge complex is included as a table

  9. Towards an integrated defense system for cyber security situation awareness experiment

    Science.gov (United States)

    Zhang, Hanlin; Wei, Sixiao; Ge, Linqiang; Shen, Dan; Yu, Wei; Blasch, Erik P.; Pham, Khanh D.; Chen, Genshe

    2015-05-01

    In this paper, an implemented defense system is demonstrated to carry out cyber security situation awareness. The developed system consists of distributed passive and active network sensors designed to effectively capture suspicious information associated with cyber threats, effective detection schemes to accurately distinguish attacks, and network actors to rapidly mitigate attacks. Based on the collected data from network sensors, image-based and signals-based detection schemes are implemented to detect attacks. To further mitigate attacks, deployed dynamic firewalls on hosts dynamically update detection information reported from the detection schemes and block attacks. The experimental results show the effectiveness of the proposed system. A future plan to design an effective defense system is also discussed based on system theory.

  10. The Tumorigenic Roles of the Cellular REDOX Regulatory Systems

    Science.gov (United States)

    Castaldo, Stéphanie Anaís; Freitas, Joana Raquel; Conchinha, Nadine Vasconcelos; Madureira, Patrícia Alexandra

    2016-01-01

    The cellular REDOX regulatory systems play a central role in maintaining REDOX homeostasis that is crucial for cell integrity, survival, and proliferation. To date, a substantial amount of data has demonstrated that cancer cells typically undergo increasing oxidative stress as the tumor develops, upregulating these important antioxidant systems in order to survive, proliferate, and metastasize under these extreme oxidative stress conditions. Since a large number of chemotherapeutic agents currently used in the clinic rely on the induction of ROS overload or change of ROS quality to kill the tumor, the cancer cell REDOX adaptation represents a significant obstacle to conventional chemotherapy. In this review we will first examine the different factors that contribute to the enhanced oxidative stress generally observed within the tumor microenvironment. We will then make a comprehensive assessment of the current literature regarding the main antioxidant proteins and systems that have been shown to be positively associated with tumor progression and chemoresistance. Finally we will make an analysis of commonly used chemotherapeutic drugs that induce ROS. The current knowledge of cancer cell REDOX adaptation raises the issue of developing novel and more effective therapies for these tumors that are usually resistant to conventional ROS inducing chemotherapy. PMID:26682014

  11. The Tumorigenic Roles of the Cellular REDOX Regulatory Systems.

    Science.gov (United States)

    Castaldo, Stéphanie Anaís; Freitas, Joana Raquel; Conchinha, Nadine Vasconcelos; Madureira, Patrícia Alexandra

    2016-01-01

    The cellular REDOX regulatory systems play a central role in maintaining REDOX homeostasis that is crucial for cell integrity, survival, and proliferation. To date, a substantial amount of data has demonstrated that cancer cells typically undergo increasing oxidative stress as the tumor develops, upregulating these important antioxidant systems in order to survive, proliferate, and metastasize under these extreme oxidative stress conditions. Since a large number of chemotherapeutic agents currently used in the clinic rely on the induction of ROS overload or change of ROS quality to kill the tumor, the cancer cell REDOX adaptation represents a significant obstacle to conventional chemotherapy. In this review we will first examine the different factors that contribute to the enhanced oxidative stress generally observed within the tumor microenvironment. We will then make a comprehensive assessment of the current literature regarding the main antioxidant proteins and systems that have been shown to be positively associated with tumor progression and chemoresistance. Finally we will make an analysis of commonly used chemotherapeutic drugs that induce ROS. The current knowledge of cancer cell REDOX adaptation raises the issue of developing novel and more effective therapies for these tumors that are usually resistant to conventional ROS inducing chemotherapy. PMID:26682014

  12. The Tumorigenic Roles of the Cellular REDOX Regulatory Systems

    Directory of Open Access Journals (Sweden)

    Stéphanie Anaís Castaldo

    2016-01-01

    Full Text Available The cellular REDOX regulatory systems play a central role in maintaining REDOX homeostasis that is crucial for cell integrity, survival, and proliferation. To date, a substantial amount of data has demonstrated that cancer cells typically undergo increasing oxidative stress as the tumor develops, upregulating these important antioxidant systems in order to survive, proliferate, and metastasize under these extreme oxidative stress conditions. Since a large number of chemotherapeutic agents currently used in the clinic rely on the induction of ROS overload or change of ROS quality to kill the tumor, the cancer cell REDOX adaptation represents a significant obstacle to conventional chemotherapy. In this review we will first examine the different factors that contribute to the enhanced oxidative stress generally observed within the tumor microenvironment. We will then make a comprehensive assessment of the current literature regarding the main antioxidant proteins and systems that have been shown to be positively associated with tumor progression and chemoresistance. Finally we will make an analysis of commonly used chemotherapeutic drugs that induce ROS. The current knowledge of cancer cell REDOX adaptation raises the issue of developing novel and more effective therapies for these tumors that are usually resistant to conventional ROS inducing chemotherapy.

  13. Iterative Cellular Screening System for Nanoparticle Safety Testing

    Directory of Open Access Journals (Sweden)

    Franziska Sambale

    2015-01-01

    Full Text Available Nanoparticles have the potential to exhibit risks to human beings and to the environment; due to the wide applications of nanoproducts, extensive risk management must not be neglected. Therefore, we have constructed a cell-based, iterative screening system to examine a variety of nanoproducts concerning their toxicity during development. The sensitivity and application of various cell-based methods were discussed and proven by applying the screening to two different nanoparticles: zinc oxide and titanium dioxide nanoparticles. They were used as benchmarks to set up our methods and to examine their effects on mammalian cell lines. Different biological processes such as cell viability, gene expression of interleukin-8 and heat shock protein 70, as well as morphology changes were investigated. Within our screening system, both nanoparticle suspensions and coatings can be tested. Electric cell impedance measurements revealed to be a good method for online monitoring of cellular behavior. The implementation of three-dimensional cell culture is essential to better mimic in vivo conditions. In conclusion, our screening system is highly efficient, cost minimizing, and reduces the need for animal studies.

  14. 47 CFR 90.672 - Unacceptable interference to non-cellular 800 MHz licensees from 800 MHz cellular systems or part...

    Science.gov (United States)

    2010-10-01

    ... Business/Industrial Land Transportation Pool. (a) Definition. Except as provided in 47 CFR 90.617(k... 47 Telecommunication 5 2010-10-01 2010-10-01 false Unacceptable interference to non-cellular 800 MHz licensees from 800 MHz cellular systems or part 22 Cellular Radiotelephone systems, and within...

  15. Motivational cues predict the defensive system in team handball: A model based on regulatory focus theory.

    Science.gov (United States)

    Debanne, T; Laffaye, G

    2015-08-01

    This study was based on the naturalistic decision-making paradigm and regulatory focus theory. Its aim was to model coaches' decision-making processes for handball teams' defensive systems based on relevant cues of the reward structure, and to determine the weight of each of these cues. We collected raw data by video-recording 41 games that were selected using a simple random method. We considered the defensive strategy (DEF: aligned or staged) to be the dependent variable, and the three independent variables were (a) numerical difference between the teams; (b) score difference between the teams; and (c) game periods. We used a logistic regression design (logit model) and a multivariate logistic model to explain the link between DEF and the three category independent variables. Each factor was weighted differently during the decision-making process to select the defensive system, and combining these variables increased the impact on this process; for instance, a staged defense is 43 times more likely to be chosen during the final period in an unfavorable situation and in a man advantage. Finally, this shows that the coach's decision-making process could be based on a simple match or could require a diagnosis of the situation based on the relevant cues. PMID:25262855

  16. Analysis and design of a cooperative weapon assignment module for advanced battle manager of a ballistic missile defense system

    OpenAIRE

    Brown, Willie D.

    2006-01-01

    The United States is in the midst of an ambitious effort to build and deploy a wide range of ballistic missile defense systems. These ballistic missile defense systems will be effective against a host of current and postulated threats from ballistic missiles. In this thesis study, we explore the process of enhancing the effectiveness of weapon assignment for a system of systems. First, analysis of information is drawn from current proposed system of the ABM and its construction from the ...

  17. Role of Nrf2 antioxidant defense in mitigating cadmium-induced oxidative stress in the olfactory system of zebrafish

    International Nuclear Information System (INIS)

    Exposure to trace metals can disrupt olfactory function in fish leading to a loss of behaviors critical to survival. Cadmium (Cd) is an olfactory toxicant that elicits cellular oxidative stress as a mechanism of toxicity while also inducing protective cellular antioxidant genes via activation of the nuclear factor (erythroid-derived 2)-like 2 (Nrf2) pathway. However, the molecular mechanisms of Cd-induced olfactory injury have not been characterized. In the present study, we investigated the role of the Nrf2-mediated antioxidant defense pathway in protecting against Cd-induced olfactory injury in zebrafish. A dose-dependent induction of Nrf2-regulated antioxidant genes associated with cellular responses to oxidative stress was observed in the olfactory system of adult zebrafish following 24 h Cd exposure. Zebrafish larvae exposed to Cd for 3 h showed increased glutathione S-transferase pi (gst pi), glutamate–cysteine ligase catalytic subunit (gclc), heme oxygenase 1 (hmox1) and peroxiredoxin 1 (prdx1) mRNA levels indicative of Nrf2 activation, and which were blocked by morpholino-mediated Nrf2 knockdown. The inhibition of antioxidant gene induction in Cd-exposed Nrf2 morphants was associated with disruption of olfactory driven behaviors, increased cell death and loss of olfactory sensory neurons (OSNs). Nrf2 morphants also exhibited a downregulation of OSN-specific genes after Cd exposure. Pre-incubation of embryos with sulforaphane (SFN) partially protected against Cd-induced olfactory tissue damage. Collectively, our results indicate that oxidative stress is an important mechanism of Cd-mediated injury in the zebrafish olfactory system. Moreover, the Nrf2 pathway plays a protective role against cellular oxidative damage and is important in maintaining zebrafish olfactory function. -- Highlights: ► Oxidative stress is an important mechanism of Cd-mediated olfactory injury. ► Cd induces antioxidant gene expression in the zebrafish olfactory system. ► The

  18. Role of Nrf2 antioxidant defense in mitigating cadmium-induced oxidative stress in the olfactory system of zebrafish

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Lu; Gallagher, Evan P., E-mail: evang3@uw.edu

    2013-01-15

    Exposure to trace metals can disrupt olfactory function in fish leading to a loss of behaviors critical to survival. Cadmium (Cd) is an olfactory toxicant that elicits cellular oxidative stress as a mechanism of toxicity while also inducing protective cellular antioxidant genes via activation of the nuclear factor (erythroid-derived 2)-like 2 (Nrf2) pathway. However, the molecular mechanisms of Cd-induced olfactory injury have not been characterized. In the present study, we investigated the role of the Nrf2-mediated antioxidant defense pathway in protecting against Cd-induced olfactory injury in zebrafish. A dose-dependent induction of Nrf2-regulated antioxidant genes associated with cellular responses to oxidative stress was observed in the olfactory system of adult zebrafish following 24 h Cd exposure. Zebrafish larvae exposed to Cd for 3 h showed increased glutathione S-transferase pi (gst pi), glutamate–cysteine ligase catalytic subunit (gclc), heme oxygenase 1 (hmox1) and peroxiredoxin 1 (prdx1) mRNA levels indicative of Nrf2 activation, and which were blocked by morpholino-mediated Nrf2 knockdown. The inhibition of antioxidant gene induction in Cd-exposed Nrf2 morphants was associated with disruption of olfactory driven behaviors, increased cell death and loss of olfactory sensory neurons (OSNs). Nrf2 morphants also exhibited a downregulation of OSN-specific genes after Cd exposure. Pre-incubation of embryos with sulforaphane (SFN) partially protected against Cd-induced olfactory tissue damage. Collectively, our results indicate that oxidative stress is an important mechanism of Cd-mediated injury in the zebrafish olfactory system. Moreover, the Nrf2 pathway plays a protective role against cellular oxidative damage and is important in maintaining zebrafish olfactory function. -- Highlights: ► Oxidative stress is an important mechanism of Cd-mediated olfactory injury. ► Cd induces antioxidant gene expression in the zebrafish olfactory system. ► The

  19. Risk Assessment Using The Homeland-Defense Operational Planning System (HOPS)

    International Nuclear Information System (INIS)

    The Homeland-Defense Operational Planning System (HOPS), is a new operational planning tool leveraging Lawrence Livermore National Laboratory's expertise in weapons systems and in sparse information analysis to support the defense of the U.S. homeland. HOPS provides planners with a basis to make decisions to protect against acts of terrorism, focusing on the defense of facilities critical to U.S. infrastructure. Criticality of facilities, structures, and systems is evaluated on a composite matrix of specific projected casualty, economic, and sociopolitical impact bins. Based on these criteria, significant unidentified vulnerabilities are identified and secured. To provide insight into potential successes by malevolent actors, HOPS analysts strive to base their efforts mainly on unclassified open-source data. However, more cooperation is needed between HOPS analysts and facility representatives to provide an advantage to those whose task is to defend these facilities. Evaluated facilities include: refineries, major ports, nuclear power plants and other nuclear licensees, dams, government installations, convention centers, sports stadiums, tourist venues, and public and freight transportation systems. A generalized summary of analyses of U.S. infrastructure facilities will be presented

  20. Use of the Homeland-Defense Operational Planning System (HOPS) for Emergency Management

    International Nuclear Information System (INIS)

    The Homeland-Defense Operational Planning System (HOPS), is a new operational planning tool leveraging Lawrence Livermore National Laboratory's expertise in weapons systems and in sparse information analysis to support the defense of the U.S. homeland. HOPS provides planners with a basis to make decisions to protect against acts of terrorism, focusing on the defense of facilities critical to U.S. infrastructure. Criticality of facilities, structures, and systems is evaluated on a composite matrix of specific projected casualty, economic, and sociopolitical impact bins. Based on these criteria, significant unidentified vulnerabilities are identified and secured. To provide insight into potential successes by malevolent actors, HOPS analysts strive to base their efforts mainly on unclassified open-source data. However, more cooperation is needed between HOPS analysts and facility representatives to provide an advantage to those whose task is to defend these facilities. Evaluated facilities include: refineries, major ports, nuclear power plants and other nuclear licensees, dams, government installations, convention centers, sports stadiums, tourist venues, and public and freight transportation systems. A generalized summary of analyses of U.S. infrastructure facilities will be presented

  1. Safety assessment of inter-channel / inter-system digital communications: A defensive measures approach

    International Nuclear Information System (INIS)

    Inappropriately designed inter-channel and inter-system digital communications could initiate common cause failure of multiple channels or multiple systems. Defensive measures were introduced in EPRI report TR-1002835 (Guideline for Performing Defense-in-Depth and Diversity Assessments for Digital Upgrades) to assess, on a deterministic basis, the susceptibility of digital systems architectures to common-cause failures. This paper suggests how this approach could be applied to assess inter-channel and inter-system digital communications from a safety standpoint. The first step of the approach is to systematically identify the so called 'influence factors' that one end of the data communication path can have on the other. Potential factors to be considered would typically include data values, data volumes and data rates. The second step of the approach is to characterize the ways possible failures of a given end of the communication path could affect these influence factors (e.g., incorrect data values, excessive data rates, time-outs, incorrect data volumes). The third step is to analyze the designed-in measures taken to guarantee independence of the other end. In addition to classical error detection and correction codes, typical defensive measures are one-way data communication, fixed-rate data communication, fixed-volume data communication, validation of data values. (authors)

  2. Use of the Homeland-Defense Operational Planning System (HOPS) for Emergency Management

    Energy Technology Data Exchange (ETDEWEB)

    Durling, Jr., R L; Price, D E

    2005-12-16

    The Homeland-Defense Operational Planning System (HOPS), is a new operational planning tool leveraging Lawrence Livermore National Laboratory's expertise in weapons systems and in sparse information analysis to support the defense of the U.S. homeland. HOPS provides planners with a basis to make decisions to protect against acts of terrorism, focusing on the defense of facilities critical to U.S. infrastructure. Criticality of facilities, structures, and systems is evaluated on a composite matrix of specific projected casualty, economic, and sociopolitical impact bins. Based on these criteria, significant unidentified vulnerabilities are identified and secured. To provide insight into potential successes by malevolent actors, HOPS analysts strive to base their efforts mainly on unclassified open-source data. However, more cooperation is needed between HOPS analysts and facility representatives to provide an advantage to those whose task is to defend these facilities. Evaluated facilities include: refineries, major ports, nuclear power plants and other nuclear licensees, dams, government installations, convention centers, sports stadiums, tourist venues, and public and freight transportation systems. A generalized summary of analyses of U.S. infrastructure facilities will be presented.

  3. Risk Assessment Using The Homeland-Defense Operational Planning System (HOPS)

    Energy Technology Data Exchange (ETDEWEB)

    Price, D E; Durling, R L

    2005-10-10

    The Homeland-Defense Operational Planning System (HOPS), is a new operational planning tool leveraging Lawrence Livermore National Laboratory's expertise in weapons systems and in sparse information analysis to support the defense of the U.S. homeland. HOPS provides planners with a basis to make decisions to protect against acts of terrorism, focusing on the defense of facilities critical to U.S. infrastructure. Criticality of facilities, structures, and systems is evaluated on a composite matrix of specific projected casualty, economic, and sociopolitical impact bins. Based on these criteria, significant unidentified vulnerabilities are identified and secured. To provide insight into potential successes by malevolent actors, HOPS analysts strive to base their efforts mainly on unclassified open-source data. However, more cooperation is needed between HOPS analysts and facility representatives to provide an advantage to those whose task is to defend these facilities. Evaluated facilities include: refineries, major ports, nuclear power plants and other nuclear licensees, dams, government installations, convention centers, sports stadiums, tourist venues, and public and freight transportation systems. A generalized summary of analyses of U.S. infrastructure facilities will be presented.

  4. Neurogenesis of cough, other airway defensive behaviors and breathing: A holarchical system?☆

    OpenAIRE

    Bolser, Donald C.; Poliacek, Ivan; Jakus, Jan; Fuller, David D.; Paul W Davenport

    2006-01-01

    Cough and breathing are generated by a common muscular system. However, these two behaviors differ significantly in their mechanical features and regulation. The current conceptualization of the neurogenic mechanism for these behaviors holds that the multifunctional respiratory pattern generator undergoes reconfiguration to produce cough. Our previous results indicate the presence of a functional cough gate mechanism that controls the excitability of this airway defensive behavior, but is not...

  5. The antioxidant defense system of Varroa destructor mites facilitates the infestation of Apis mellifera

    Directory of Open Access Journals (Sweden)

    Dmochowska-Ślęzak Kamila

    2016-06-01

    Full Text Available Varroa destructor is a parasitic mite of the Western honey bee. The activity of five antioxidant enzymes of V. destructor were analysed. Glutathione content and total antioxidant status was also evaluated. Our results suggest that antioxidant enzymes constitute the main line of defense against ROS in V. destructor, whereas low-molecular-weight antioxidants play a limited role in the antioxidant system of mites.

  6. Defense and intelligence technologies applied to law enforcement: remote consultation information system (RCIS)

    Science.gov (United States)

    Penninger, Robert B.

    1995-05-01

    The Remote Consultation Information System is a body-worn fully integrated communication system that provide secure two-way voice-quality audio, secure full-color video transmission, automatic position location (APL) via Global Positioning System, and vital life signs moitoring, all linked to an adaptable base station via spread-spectrum low-probability-of- intercept, Broadband Code Division Multiple Access radio link. All of these technologies have been developed by the defense and intelligence communities and are now availbale for law enforcement agencies.

  7. The similia principle: results obtained in a cellular model system.

    Science.gov (United States)

    Wiegant, Fred; Van Wijk, Roeland

    2010-01-01

    This paper describes the results of a research program focused on the beneficial effect of low dose stress conditions that were applied according to the similia principle to cells previously disturbed by more severe stress conditions. In first instance, we discuss criteria for research on the similia principle at the cellular level. Then, the homologous ('isopathic') approach is reviewed, in which the initial (high dose) stress used to disturb cellular physiology and the subsequent (low dose) stress are identical. Beneficial effects of low dose stress are described in terms of increased cellular survival capacity and at the molecular level as an increase in the synthesis of heat shock proteins (hsps). Both phenomena reflect a stimulation of the endogenous cellular self-recovery capacity. Low dose stress conditions applied in a homologous approach stimulate the synthesis of hsps and enhance survival in comparison with stressed cells that were incubated in the absence of low dose stress conditions. Thirdly, the specificity of the low dose stress condition is described where the initial (high dose) stress is different in nature from the subsequently applied (low dose) stress; the heterologous or 'heteropathic' approach. The results support the similia principle at the cellular level and add to understanding of how low dose stress conditions influence the regulatory processes underlying self-recovery. In addition, the phenomenon of 'symptom aggravation' which is also observed at the cellular level, is discussed in the context of self-recovery. Finally, the difference in efficiency between the homologous and the heterologous approach is discussed; a perspective is indicated for further research; and the relationship between studies on the similia principle and the recently introduced concept of 'postconditioning hormesis' is emphasized. PMID:20129172

  8. Protective Effects of Zinc Against Acute Arsenic Toxicity by Regulating Antioxidant Defense System and Cumulative Metallothionein Expression.

    Science.gov (United States)

    Ganger, Renuka; Garla, Roobee; Mohanty, Biraja Prasad; Bansal, Mohinder Pal; Garg, Mohan Lal

    2016-02-01

    Arsenic (As), a toxic metalloid, is one of the major global concerns. The toxicity resulting from As exposure is linked to the generation of reactive oxygen intermediates during their redox cycling and metabolic activation processes that cause lipid peroxidation (LPO). Zinc (Zn), a redox-inactive metal, helps to maintain cellular functions because of its prominent role in antioxidant network through multiple mechanisms. The present study, therefore, explores the effectiveness of administered Zn to combat against acute As toxicity by analysis of antioxidant defense status, alkaline phosphatase (ALP) activity, histological profile, MT expression, and elemental status in rat liver. To achieve this goal, four experimental groups, one control and three receiving different metal supplementations, were chosen (group 1, control; group 2, Zn supplemented; group 3, As substituted; group 4, Zn + As supplemented). The levels of reduced glutathione (GSH) and activities of glutathione reductase (GR) and ALP were lowered, whereas LPO levels and activity of superoxide dismutase (SOD) were elevated with no significant change in catalase (CAT) activity. Histopathological changes were also observed in the As substituted group in comparison to the control. Particle-induced X-ray emission (PIXE) analysis showed decrease in Fe and S concentration in rat liver after As intoxication, whereas As was below detection limit, i.e., <1 ppm. Zn administration almost restored the antioxidants, ALP activity, histopathological changes, and elemental status. A cumulative increase in MT expression was found with the combined treatment of Zn and As. Also, Zn alone caused no significant change in the antioxidant defense system. It can be concluded that restoration of antioxidant activity and increased MT expression are the two independent protective mechanisms of Zn to reduce acute As toxicity. PMID:26113309

  9. Fröhlich systems in cellular physiology

    Czech Academy of Sciences Publication Activity Database

    Šrobár, Fedor

    2012-01-01

    Roč. 113, č. 2 (2012), s. 95-104. ISSN 1214-6994 R&D Projects: GA ČR(CZ) GAP102/11/0649 Institutional support: RVO:67985882 Keywords : Biophysics * Cellular Physiology Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering

  10. Whole-Organism Cellular Pathology: A Systems Approach to Phenomics.

    Science.gov (United States)

    Cheng, K C; Katz, S R; Lin, A Y; Xin, X; Ding, Y

    2016-01-01

    Phenotype is defined as the state of an organism resulting from interactions between genes, environment, disease, molecular mechanisms, and chance. The purpose of the emerging field of phenomics is to systematically determine and measure phenotypes across biology for the sake of understanding. Phenotypes can affect more than one cell type and life stage, so ideal phenotyping would include the state of every cell type within the context of both tissue architecture and the whole organism at each life stage. In medicine, high-resolution anatomic assessment of phenotype is obtained from histology. Histology's interpretative power, codified by Virchow as cellular pathology, is derived from its ability to discern diagnostic and characteristic cellular changes in diseased tissues. Cellular pathology is observed in every major human disease and relies on the ability of histology to detect cellular change in any cell type due to unbiased pan-cellular staining, even in optically opaque tissues. Our laboratory has shown that histology is far more sensitive than stereomicroscopy for detecting phenotypes in zebrafish mutants. Those studies have also shown that more complete sampling, greater consistency in sample orientation, and the inclusion of phenotypes extending over longer length scales would provide greater coverage of common phenotypes. We are developing technical approaches to achieve an ideal detection of cellular pathology using an improved form of X-ray microtomography that retains the strengths and addresses the weaknesses of histology as a screening tool. We are using zebrafish as a vertebrate model based on the overlaps between zebrafish and mammalian tissue architecture, and a body size small enough to allow whole-organism, volumetric imaging at cellular resolution. Automation of whole-organism phenotyping would greatly increase the value of phenomics. Potential societal benefits would include reduction in the cost of drug development, a reduction in the

  11. Augmenting defense-in-depth with the concepts of observability and diagnosability from Control Theory and Discrete Event Systems

    International Nuclear Information System (INIS)

    Defense-in-depth is a fundamental principle/strategy for achieving system safety. First conceptualized within the nuclear industry, defense-in-depth is the basis for risk-informed decisions by the U.S. Nuclear Regulatory Commission, and is recognized under various names in other industries (e.g., layers of protection in the Chemical industry). Accidents typically result from the absence or breach of defenses or violation of safety constraints. Defense-in-depth is realized by a diversity of safety barriers and a network of redundancies. However, this same redundancy and the intrinsic nature of defense-in-depth - the multiple lines of defense or 'protective layers' along a potential accident sequence - may enhance mechanisms concealing the occurrence of incidents, or that the system has transitioned to a hazardous state (accident pathogens) and that an accident is closer to being released. Consequently, the ability to safely operate the system may be hampered and the efficiency of defense-in-depth may be degraded or worse may backfire. Several accidents reports identified hidden failures or degraded observability of accidents pathogens as major contributing factors. In this work, we begin to address this potential theoretical deficiency in defense-in-depth by bringing concepts from Control Theory and Discrete Event Systems to bear on issues of system safety and accident prevention. We introduce the concepts of controllability, observability, and diagnosability, and frame the current understanding of system safety as a 'control problem' handled by defense-in-depth and safety barriers (or safety constraints). Observability and diagnosability are information-theoretic concepts, and they provide important complements to the energy model of accident causation from which the defense-in-depth principle derives. We formulate a new safety-diagnosability principle for supporting accident prevention, and propose that defense-in-depth be augmented with this principle, without

  12. Department of Defense picture archiving and communication system acceptance testing: results and identification of problem components.

    Science.gov (United States)

    Allison, Scott A; Sweet, Clifford F; Beall, Douglas P; Lewis, Thomas E; Monroe, Thomas

    2005-09-01

    The PACS implementation process is complicated requiring a tremendous amount of time, resources, and planning. The Department of Defense (DOD) has significant experience in developing and refining PACS acceptance testing (AT) protocols that assure contract compliance, clinical safety, and functionality. The DOD's AT experience under the initial Medical Diagnostic Imaging Support System contract led to the current Digital Imaging Network-Picture Archiving and Communications Systems (DIN-PACS) contract AT protocol. To identify the most common system and component deficiencies under the current DIN-PACS AT protocol, 14 tri-service sites were evaluated during 1998-2000. Sixteen system deficiency citations with 154 separate types of limitations were noted with problems involving the workstation, interfaces, and the Radiology Information System comprising more than 50% of the citations. Larger PACS deployments were associated with a higher number of deficiencies. The most commonly cited systems deficiencies were among the most expensive components of the PACS. PMID:15924273

  13. Station Model for Rail Transit System Using Cellular Automata

    Institute of Scientific and Technical Information of China (English)

    XUN Jing; NING Bin; LI Ke-Ping

    2009-01-01

    In this paper, we propose a new cellular automata model to simulate the railway traffic at station.Based on NaSch model, the proposed station model is composed of the main track and the siding track.Two different schemes for trains passing through station are considered.One is the scheme of "pass by the main track, start and stop by the siding track".The other is the scheme of "two tracks play the same role".We simulate the train movement using the proposed model and analyze the traffic flow at station.The simulation results demonstrate that the proposed cellular automata model can be successfully used for the simulations of railway traffic.Some characteristic behaviors of railway traffic flow can be reproduced.Moreover, the simulation values of the minimum headway are close to the theoretical values.This result demonstrates the dependability and availability of the proposed model.

  14. Cellular Manufacturing Systems: Organization, Trends And Innovative Methods

    OpenAIRE

    İŞLİER, Attila

    2015-01-01

    Interchangeability is the distinguishing feature of modern manufacturing. A huge production capacity to satisfy the people is reached thanks to that concept. Yet that prospect brought up a dilemma too. Efficient but inflexible flow lines for very limited product types on one side and unproductive flexible batch production for numerous diverse parts on the other side. The remedy is thought of as Cellular Manufacturing (CM). That seemed a brilliant idea but the proliferation of CM has never rea...

  15. Diversity and Multiplexing Tradeoff in the Uplink of Cellular Systems with Linear MMSE Receiver

    CERN Document Server

    Song, Xiaoshi; Liu, Danpu

    2011-01-01

    In this paper, we extend the diversity and multiplexing tradeoff (DMT) analysis from point-to-point channels to cellular systems to evaluate the impact of inter-cell interference on the system reliability and efficiency. Fundamental tradeoff among diversity order, multiplexing gain and inter-cell interference intensity is characterized to reveal the capability of multiple antennas in cellular systems. And the detrimental effects of the inter-cell interference on the system performance of diversity and multiplexing is presented and analyzed.

  16. Brassinosteroids Denigrate the Seasonal Stress through Antioxidant Defense System in Seedlings of Brassica juncea L.

    OpenAIRE

    Sandeep Kumar; Geetika Sirhindi; Renu Bhardwaj; Manish Kumar

    2014-01-01

    The present work has been undertaken to study the effect of exogenously application of 24-epiBL and 28-homoBL on soluble protein, proline contents and antioxidant defense system of Brassica juncea L. RLM 619 under the influence of seasonal stress. It was observed that 24-epiBL and 28-homoBL treatment enhance the soluble protein, dry weight and shoot length of B. juncea seedlings under seasonal stress. If seeds treated with the different concentrations (10-6, 10-8 and 10-10 M) of 24-epiBL and ...

  17. BRASSINOSTEROIDS DENIGRATE THE SEASONAL STRESS THROUGH ANTIOXIDANT DEFENSE SYSTEM IN SEEDLINGS OF BRASSICA JUNCEA L

    OpenAIRE

    Kumar, Sandeep; Sirhindi, Geetika; Bhardwaj, Renu; Kumar, Manish

    2014-01-01

    The present work has been undertaken to study the effect of exogenously application of 24-epiBL and 28-homoBL on soluble protein, proline contents and antioxidant defense system of Brassica juncea L. RLM 619 under the influence of seasonal stress. It was observed that 24-epiBL and 28-homoBL treatment enhance the soluble protein, dry weight and shoot length of B. juncea seedlings under seasonal stress. If seeds treated with the different concentrations (10 -6, 10 -8 and 10 -10 M) of 24-epiBL a...

  18. Kinetic Monte Carlo and Cellular Particle Dynamics Simulations of Multicellular Systems

    CERN Document Server

    Flenner, Elijah; Barz, Bogdan; Neagu, Adrian; Forgacs, Gabor; Kosztin, Ioan

    2011-01-01

    Computer modeling of multicellular systems has been a valuable tool for interpreting and guiding in vitro experiments relevant to embryonic morphogenesis, tumor growth, angiogenesis and, lately, structure formation following the printing of cell aggregates as bioink particles. Computer simulations based on Metropolis Monte Carlo (MMC) algorithms were successful in explaining and predicting the resulting stationary structures (corresponding to the lowest adhesion energy state). Here we introduce two alternatives to the MMC approach for modeling cellular motion and self-assembly: (1) a kinetic Monte Carlo (KMC), and (2) a cellular particle dynamics (CPD) method. Unlike MMC, both KMC and CPD methods are capable of simulating the dynamics of the cellular system in real time. In the KMC approach a transition rate is associated with possible rearrangements of the cellular system, and the corresponding time evolution is expressed in terms of these rates. In the CPD approach cells are modeled as interacting cellular ...

  19. Modeling chemical systems using cellular automata a textbook and laboratory manual

    CERN Document Server

    Kier, Lemont B; Cheng, Chao-Kun

    2006-01-01

    Provides a practical introduction to an exciting modeling paradigm for complex systems. This book discusses the nature of scientific inquiry using models and simulations, and describes the nature of cellular automata models. It gives descriptions of how cellular automata models can be used in the study of a variety of phenomena.

  20. TRUSYSTEM - A computerized system for the management of defense transuranic waste

    International Nuclear Information System (INIS)

    The U.S. Department of Energy (DOE) is embarking on a program to dispose of defense transuranic waste in the Waste Isolation Pilot Plant (WIPP) near Carlsbad, NM. In order to ensure that WIPP receives the waste at the required rate, the careful management of a logistic system involving numerous DOE facilities and specialized waste transports is required. TRUSYSTEM is being used in the design and operation of this complex system. TRUSYSTEM consists of a computerized database (PRIMS), a system simulation program (TRUSIM), and software for the monitoring and dispatching of waste transports (PHOENIX). This system designed to operate on the new generation of microcomputers, is being written in machine independent code (FORTRAN and ''C''). It is easily maintained and can be moved between different types of computers. TRUSYSTEM resides entirely within computer memory. This provides the speed necessary for real-time operations

  1. TRUSYSTEM: A computerized system for the management of defense transuranic waste

    International Nuclear Information System (INIS)

    The US Department of Energy (DOE) is embarking on a program to dispose of defense transuranic waste in the Waste Isolation Pilot Plant (WIPP) near Carlsbad, NM. In order to ensure that WIPP receives the waste at the required rate, the careful management of a logistic system involving numerous DOE facilities and specialized transports is required. TRUSYSTEM is being used in the design and operation of this complex system. TRUSYSTEM consists of a computerized database, a system simulation program, and software for the monitoring and dispatching of waste transport. This system, designed to operate on new generation of microcomputers, is being written in machine independent code (FORTRAN and ''C''). It is easily maintained and can be moved among different types of computers. TRUSYSTEM resides entirely within computer memory. This provides the speed necessary for real-time operations

  2. Method for performing diversity and defense-in-depth analyses of reactor protection systems

    International Nuclear Information System (INIS)

    The purpose of this NUREG is to describe a method for analyzing computer-based nuclear reactor protection systems that discovers design vulnerabilities to common-mode failure. The potential for common-mode failure has become an important issue as the software content of protection systems has increased. This potential was not present in earlier analog protection systems because it could usually be assumed that common-mode failure, if it did occur, was due to slow processes such as corrosion or premature wear-out. This assumption is no longer true for systems containing software. It is the purpose of the analysis method described here to determine points of a design for which credible common-mode failures are uncompensated either by diversity or defense-in-depth

  3. A unique cellular scaling rule in the avian auditory system.

    Science.gov (United States)

    Corfield, Jeremy R; Long, Brendan; Krilow, Justin M; Wylie, Douglas R; Iwaniuk, Andrew N

    2016-06-01

    Although it is clear that neural structures scale with body size, the mechanisms of this relationship are not well understood. Several recent studies have shown that the relationship between neuron numbers and brain (or brain region) size are not only different across mammalian orders, but also across auditory and visual regions within the same brains. Among birds, similar cellular scaling rules have not been examined in any detail. Here, we examine the scaling of auditory structures in birds and show that the scaling rules that have been established in the mammalian auditory pathway do not necessarily apply to birds. In galliforms, neuronal densities decrease with increasing brain size, suggesting that auditory brainstem structures increase in size faster than neurons are added; smaller brains have relatively more neurons than larger brains. The cellular scaling rules that apply to auditory brainstem structures in galliforms are, therefore, different to that found in primate auditory pathway. It is likely that the factors driving this difference are associated with the anatomical specializations required for sound perception in birds, although there is a decoupling of neuron numbers in brain structures and hair cell numbers in the basilar papilla. This study provides significant insight into the allometric scaling of neural structures in birds and improves our understanding of the rules that govern neural scaling across vertebrates. PMID:26002617

  4. Configuration of Risk Monitor System by PLant Defense-In.Depth Monitor and Relability Monitor

    DEFF Research Database (Denmark)

    Yoshikawa, Hidekazu; Lind, Morten; Yang, Ming;

    2012-01-01

    A new method of risk monitor system of a nuclear power plant has been proposed from the aspect by what degree of safety functions incorporated in the plant system is maintained by multiple barriers of defense-in-depth (DiD). Wherein, the central idea is plant DiD risk monitor and reliability...... monitor derived from the four aspects of (i) design principle of nuclear safety to realize DiD concept, (ii) definition of risk and risk to be monitored, (iii) severe accident phenomena as major risk, (iv) scheme of risk ranking, and (v) dynamic risk display. In this paper, the overall frame of the...... proposed frame on risk monitor system is summarized and the detailed discussion is made on the definitions of major terminologies of risk, risk ranking, anatomy of fault occurrence, two-layer configuration of risk monitor, how to configure individual elements of plant DiD risk monitor and its example...

  5. Bifurcation and complex dynamics of a discrete-time predator-prey system involving group defense

    Directory of Open Access Journals (Sweden)

    S. M. Sohel Rana

    2015-09-01

    Full Text Available In this paper, we investigate the dynamics of a discrete-time predator-prey system involving group defense. The existence and local stability of positive fixed point of the discrete dynamical system is analyzed algebraically. It is shown that the system undergoes a flip bifurcation and a Neimark-Sacker bifurcation in the interior of R+2 by using bifurcation theory. Numerical simulation results not only show the consistence with the theoretical analysis but also display the new and interesting dynamical behaviors, including phase portraits, period-7, 20-orbits, attracting invariant circle, cascade of period-doubling bifurcation from period-20 leading to chaos, quasi-periodic orbits, and sudden disappearance of the chaotic dynamics and attracting chaotic set. The Lyapunov exponents are numerically computed to characterize the complexity of the dynamical behaviors.

  6. High power and efficiency quantum cascade laser systems for defense and security applications

    Science.gov (United States)

    Tsekoun, A.; Lyakh, A.; Maulini, R.; Lane, M.; Macdonald, T.; Go, R.; Patel, C. Kumar N.

    2009-05-01

    Leveraging Pranalytica's fundamental research into high power and high wallplug efficiency QCL devices and high performance/reliability QCL packaging technologies, we developed several models of turn-key QCL systems for security-related applications. Our tabletop high power system produces, at room temperature, more than 2W of nominally collimated continuous wave radiation at 4.6 μm. Our flashlight-size portable illuminator at 4.6 μm produces over 100 mW average power portable illuminator at 9.6 μm produces more than 20 mW, both with runtime of ~10 hrs. These systems are opening the window of QCL acceptance into real-world security and defense applications. At chip level, we have demonstrated 3W of CW power at room temperature from a single, high reflectivity coated chip.

  7. Statistical analysis of nanoparticle dosing in a dynamic cellular system

    Science.gov (United States)

    Summers, Huw D.; Rees, Paul; Holton, Mark D.; Rowan Brown, M.; Chappell, Sally C.; Smith, Paul J.; Errington, Rachel J.

    2011-03-01

    The delivery of nanoparticles into cells is important in therapeutic applications and in nanotoxicology. Nanoparticles are generally targeted to receptors on the surfaces of cells and internalized into endosomes by endocytosis, but the kinetics of the process and the way in which cell division redistributes the particles remain unclear. Here we show that the chance of success or failure of nanoparticle uptake and inheritance is random. Statistical analysis of nanoparticle-loaded endosomes indicates that particle capture is described by an over-dispersed Poisson probability distribution that is consistent with heterogeneous adsorption and internalization. Partitioning of nanoparticles in cell division is random and asymmetric, following a binomial distribution with mean probability of 0.52-0.72. These results show that cellular targeting of nanoparticles is inherently imprecise due to the randomness of nature at the molecular scale, and the statistical framework offers a way to predict nanoparticle dosage for therapy and for the study of nanotoxins.

  8. Changing the paradigm: a radiation protection model for utilizing active systems in homeland defense applications

    International Nuclear Information System (INIS)

    Full text: The emerging threat from non-state actors and terrorist organizations openly asserting the use of weapons of mass destruction has led to an increased global emphasis on homeland defense and national security. The threat is real and thus many states are spending large amounts of resources to combat the problem. Issues such as unsecured radioactive sources, illicit movement of nuclear material, the use of radioactive sources in the medical and industrial sectors are just a few of the areas being examined. Countering these threats involves a major paradigm shift in the manner in which radiation is viewed. Radiation-based technologies are playing a key role in this emerging area from the development of new passive detection modalities to the use of active systems for detecting illicit materials. The screening of humans, baggage and cargo present overwhelming challenges. With these developments comes the concern from both governmental agencies and the public as to the safety of such systems. This paper will explore one such modality; the employment of radiation-based illicit material detection systems. The authors will present a review of the international (IAEA) and US regulatory and statutory documents as well as recommendations from scientific bodies such as the International Commission on Radiological Protection and the International Commission on Radiation Units and Measurements. This review coupled with sound scientific data are used to develop a proposed framework for the future employment of active systems for homeland defense applications. Issues considered include the ability to effectively measure and/or predict dose, is the As Low As Reasonably Achievable (ALARA) concept appropriate?, how much dose should be allowed relative to national security?, where does the linear-no-threshold hypothesis fit in? The authors use this analysis to develop a model which examines the trade-offs and cost-benefits in using the proposed systems. The major findings of

  9. Integration of peroxisomes into an endomembrane system that governs cellular aging

    Directory of Open Access Journals (Sweden)

    Adam eBeach

    2012-07-01

    Full Text Available The peroxisome is an organelle that has long been known for its essential roles in oxidation of fatty acids, maintenance of reactive oxygen species (ROS homeostasis and anaplerotic replenishment of tricarboxylic acid (TCA cycle intermediates destined for mitochondria. Growing evidence supports the view that these peroxisome-confined metabolic processes play an essential role in defining the replicative and chronological age of a eukaryotic cell. Much progress has recently been made in defining molecular mechanisms that link cellular aging to fatty acid oxidation, ROS turnover and anaplerotic metabolism in peroxisomes. Emergent studies have revealed that these organelles not only house longevity-defining metabolic reactions but can also regulate cellular aging via their dynamic communication with other cellular compartments. Peroxisomes communicate with other organelles by establishing extensive physical contact with lipid bodies, maintaining an endoplasmic reticulum (ER to peroxisome connectivity system, exchanging certain metabolites, and being involved in the bidirectional flow of some of their protein and lipid constituents. The scope of this review is to summarize the evidence that peroxisomes are dynamically integrated into an endomembrane system that governs cellular aging. We discuss recent progress in understanding how communications between peroxisomes and other cellular compartments within this system influence the development of a pro- or anti-aging cellular pattern. We also propose a model for the integration of peroxisomes into the endomembrane system governing cellular aging and critically evaluate several molecular mechanisms underlying such integration.

  10. DMPD: Toll-like receptors and the host defense against microbial pathogens: bringingspecificity to the innate-immune system. [Dynamic Macrophage Pathway CSML Database

    Lifescience Database Archive (English)

    Full Text Available 15075354 Toll-like receptors and the host defense against microbial pathogens: bringings... the host defense against microbial pathogens: bringingspecificity to the innate-...immune system. PubmedID 15075354 Title Toll-like receptors and the host defense against microbial pathogens: bringings

  11. Influence of attacker's target recognition ability on defense strategy in homogeneous parallel systems

    International Nuclear Information System (INIS)

    The article considers defense resource allocation in a system exposed to external intentional attack. The defender distributes its resource between deploying redundant elements and their protection from attacks. The attacker observes all the elements and tries to detect the unprotected elements. All the detected unprotected elements are destroyed with negligible effort. The attacker then distributes its effort evenly among all of the undetected elements or among elements from a chosen subset of undetected elements. The vulnerability of each element is determined by an attacker-defender contest success function depending on the resources allocated to protection and attack efforts and on the contest intensity. The expected damage caused by the attack is evaluated as system unsupplied demand. The article studies the influence of the unprotected elements' detection probability on the optimal resource distribution.

  12. Two satellite image sets for the training and validation of image processing systems for defense applications

    Science.gov (United States)

    Peterson, Michael R.; Aldridge, Shawn; Herzog, Britny; Moore, Frank

    2010-04-01

    Many image processing algorithms utilize the discrete wavelet transform (DWT) to provide efficient compression and near-perfect reconstruction of image data. Defense applications often require the transmission of data at high levels of compression over noisy channels. In recent years, evolutionary algorithms (EAs) have been utilized to optimize image transform filters that outperform standard wavelets for bandwidth-constrained compression of satellite images. The optimization of these filters requires the use of training images appropriately chosen for the image processing system's intended applications. This paper presents two robust sets of fifty images each intended for the training and validation of satellite and unmanned aerial vehicle (UAV) reconnaissance image processing algorithms. Each set consists of a diverse range of subjects consisting of cities, airports, military bases, and landmarks representative of the types of images that may be captured during reconnaissance missions. Optimized algorithms may be "overtrained" for a specific problem instance and thus exhibit poor performance over a general set of data. To reduce the risk of overtraining an image filter, we evaluate the suitability of each image as a training image. After evolving filters using each image, we assess the average compression performance of each filter across the entire set of images. We thus identify a small subset of images from each set that provide strong performance as training images for the image transform optimization problem. These images will also provide a suitable platform for the development of other algorithms for defense applications. The images are available upon request from the contact author.

  13. A new method for decreasing cell-load variation in dynamic cellular manufacturing systems

    OpenAIRE

    Aidin Delgoshaei; Mohd Khairol Mohd Ariffin,; Btht Hang Tuah Bin Baharudin; Zulkiflle Leman

    2016-01-01

    Cell load variation is considered a significant shortcoming in scheduling of cellular manufacturing systems. In this article, a new method is proposed for scheduling dynamic cellular manufacturing systems in the presence of bottleneck and parallel machines. The aim of this method is to control cell load variation during the process of determining the best trading off values between in-house manufacturing and outsourcing. A genetic algorithm (GA) is developed because of the high potential of t...

  14. Tensegrity, cellular biophysics, and the mechanics of living systems

    Science.gov (United States)

    Ingber, Donald E.; Wang, Ning; Stamenović, Dimitrije

    2014-01-01

    The recent convergence between physics and biology has led many physicists to enter the fields of cell and developmental biology. One of the most exciting areas of interest has been the emerging field of mechanobiology that centers on how cells control their mechanical properties, and how physical forces regulate cellular biochemical responses, a process that is known as mechanotransduction. In this article, we review the central role that tensegrity (tensional integrity) architecture, which depends on tensile prestress for its mechanical stability, plays in biology. We describe how tensional prestress is a critical governor of cell mechanics and function, and how use of tensegrity by cells contributes to mechanotransduction. Theoretical tensegrity models are also described that predict both quantitative and qualitative behaviors of living cells, and these theoretical descriptions are placed in context of other physical models of the cell. In addition, we describe how tensegrity is used at multiple size scales in the hierarchy of life — from individual molecules to whole living organisms — to both stabilize three-dimensional form and to channel forces from the macroscale to the nanoscale, thereby facilitating mechanochemical conversion at the molecular level. PMID:24695087

  15. Tensegrity, cellular biophysics, and the mechanics of living systems

    Science.gov (United States)

    Ingber, Donald E.; Wang, Ning; Stamenović, Dimitrije

    2014-04-01

    The recent convergence between physics and biology has led many physicists to enter the fields of cell and developmental biology. One of the most exciting areas of interest has been the emerging field of mechanobiology that centers on how cells control their mechanical properties, and how physical forces regulate cellular biochemical responses, a process that is known as mechanotransduction. In this article, we review the central role that tensegrity (tensional integrity) architecture, which depends on tensile prestress for its mechanical stability, plays in biology. We describe how tensional prestress is a critical governor of cell mechanics and function, and how use of tensegrity by cells contributes to mechanotransduction. Theoretical tensegrity models are also described that predict both quantitative and qualitative behaviors of living cells, and these theoretical descriptions are placed in context of other physical models of the cell. In addition, we describe how tensegrity is used at multiple size scales in the hierarchy of life—from individual molecules to whole living organisms—to both stabilize three-dimensional form and to channel forces from the macroscale to the nanoscale, thereby facilitating mechanochemical conversion at the molecular level.

  16. Tensegrity, cellular biophysics, and the mechanics of living systems

    International Nuclear Information System (INIS)

    The recent convergence between physics and biology has led many physicists to enter the fields of cell and developmental biology. One of the most exciting areas of interest has been the emerging field of mechanobiology that centers on how cells control their mechanical properties, and how physical forces regulate cellular biochemical responses, a process that is known as mechanotransduction. In this article, we review the central role that tensegrity (tensional integrity) architecture, which depends on tensile prestress for its mechanical stability, plays in biology. We describe how tensional prestress is a critical governor of cell mechanics and function, and how use of tensegrity by cells contributes to mechanotransduction. Theoretical tensegrity models are also described that predict both quantitative and qualitative behaviors of living cells, and these theoretical descriptions are placed in context of other physical models of the cell. In addition, we describe how tensegrity is used at multiple size scales in the hierarchy of life—from individual molecules to whole living organisms—to both stabilize three-dimensional form and to channel forces from the macroscale to the nanoscale, thereby facilitating mechanochemical conversion at the molecular level. (review article)

  17. System for retrieval of solidified Hanford high-level defense wastes: a design description

    International Nuclear Information System (INIS)

    The major objective of the Hanford defense waste retrieval activity is to design, develop, demonstrate, and qualify the equipment, staff, and methods required for the safe retrieval of solidified high-level radioactive wastes from the 133 large (75-foot-diameter) single-shell, underground storage tanks at Hanford. The program is currently limited to research, development, and demonstration of systems and technology in support of selecting an alternative for disposal of Hanford high-level defense waste. This report relates the requirements and sets forth much of the data and criteria currently known. It describes the preliminary design of a remotely operated, self-contained, mobile system which incorporates a mechanical, articulated mining boom. This boom, when equipped with a variety of suitable tools, can remotely cut up in-tank equipment, remove the debris, recover the solid wastes, clean the tank walls, and deposit the waste into a bucket elevator. The bucket elevator automatically removes the waste from the tank and deposits it into shielded shipping containers. The containers are remotely sealed, washed, and deposited into sealing containers. The containers are remotely sealed, washed, and deposited into sealing containers which are sealed and placed on a transfer vehicle for transport. All of the retrieval equipment is installed on a mobile platform which bridges the tank domes to avoid loading the dome structure. Mobility provides for progressing from tank to tank until all of the 75-foot-diameter, single-shell tanks have been emptied. Ancillary equipment includes in-tank viewing and lighting systems, positive filtered ventilation equipment for contamination control, equipment for washing tools and mining equipment, and includes provisions for remote maintenance of contaminated equipment, and quick removal and replacement of parts

  18. A Simple Scheme for Improved Performance of Fixed Outage Rate Cellular System

    Directory of Open Access Journals (Sweden)

    Hussein M.A. Basi

    2004-01-01

    Full Text Available The traffic characteristic of mobile cellular systems is rather distinct from that of a fixed telephone network. However the system planning and design are still carried out with the tools of conventional traffic theory. In the recent days much work is being done in the performance evaluation of mobile or cellular communication systems in order to develop a system with greater efficiency. The useful parameters to estimate the performance of the system are voice quality, frequency spectrum efficiency and Grade of Service (GOS. The grade of service will be affected due to outage of channels. In this study, a new scheme is proposed to reduce lost calls due to channel outage in the fixed rate outage cellular system. In this proposed scheme the call will never dropped but may be delayed. The system performance is evaluated for different conditions and the results are discussed.

  19. A novel antibody-dependent cellular cytotoxicity mechanism involved in defense against malaria requires costimulation of monocytes FcgammaRII and FcgammaRIII

    DEFF Research Database (Denmark)

    Jafarshad, Ali; Dziegiel, Morten Hanefeld; Lundquist, Rasmus; Nielsen, Leif K; Singh, Subhash; Druilhe, Pierre L

    2007-01-01

    Clinical experiments have shown that the Ab-dependent cell-mediated inhibition of Plasmodium falciparum is a major mechanism controlling malaria parasitemia and thereby symptoms. In this study, we demonstrate that a single merozoite per monocyte (MN) is sufficient to trigger optimal antiparasitic......-dependent cellular cytotoxicity and implies that all MN are not equally effective. These findings have both fundamental and practical implications, particularly for vaccine discovery....

  20. Defense-in-depth and diversity assessment of the RESAR-414 Integrated Protection System

    International Nuclear Information System (INIS)

    This report discusses the defense-in-depth and diversity principles as they apply to safety related instrumentation and presents guidelines which can be used to assess the degree to which the designs of complex, interconnected safety systems conform to these principles. These guidelines are based on the use of the block concept, an approach in which the components and modules of the system are aggregated into a small number of functional units, or blocks, to simplify the analysis. It is believed that the use of the block concept and the guidelines will result in a conservative assessment of the capability of such systems to function when subjected to postulated to common-mode failures. A preliminary assessment of the RESAR-414 Integrated Protection System by means of the guidelines is also presented. The results of this assessment support the conclusion that, for purposes of a preliminary design approval, the RESAR-414 Integrated Protection System is acceptable. However, the assessment, has also resulted in requirements for additional analyses and tests, the results of which must demonstrate conformance to the guidelines prior to the issuance of a Final Design Approval

  1. Combined activation of the energy and cellular-defense pathways may explain the potent anti-senescence activity of methylene blue

    OpenAIRE

    Atamna, Hani; Atamna, Wafa; Al-Eyd, Ghaith; Shanower, Gregory; Dhahbi, Joseph M.

    2015-01-01

    Methylene blue (MB) delays cellular senescence, induces complex-IV, and activates Keap1/Nrf2; however, the molecular link of these effects to MB is unclear. Since MB is redox-active, we investigated its effect on the NAD/NADH ratio in IMR90 cells. The transient increase in NAD/NADH observed in MB-treated cells triggered an investigation of the energy regulator AMPK. MB induced AMPK phosphorylation in a transient pattern, which was followed by the induction of PGC1α and SURF1: both are inducer...

  2. Research Progression of Cellular Autophagy in Liver System Diseases

    Directory of Open Access Journals (Sweden)

    Chunyun Liu

    2013-09-01

    Full Text Available Autophagy is a basic biological phenomenon widely existed in eukaryotic cells and an important mechanism for cells to adjust to the surrounding environment, prevent invasion of pathogenic micro-organisms and maintain homeostasis, whose activity changes evidently in multiple liver system diseases, suggesting that there is close association between autophagy and the generation and development of liver system diseases. It is also reported that autophagy develops and exerts an important function in many liver-related diseases, such as hepatic carcinoma, non-alcoholic fatty liver disease, alcoholic liver disease, viral liver disease and acute liver injury. Therefore, this study aimed to summarize the relationship between autophagy and multiple liver diseases, hoping to explore the effect of autophagy in liver system diseases and further study the regulative effect of autophagy so as to provide new thoughts for their treatment.

  3. Defense Travel System use of restricted airfare in conjunction with the GSA City Pair Program to effectively reduce TDY travel costs within DoD

    OpenAIRE

    Feehan, Ashton F.

    2008-01-01

    This thesis discusses the Naval Postgraduate School's use of the Defense Travel System (DTS) and examines a process of using the GSA City Pair Program in conjunction with restricted airfare as a means to effectively reduce travel costs and conserve Department of Defense dollars. The thesis starts off with a brief introduction of travel at NPS, and is followed by a section on the Defense Travel System to include NPS's history with electronic travel systems. The third chapter highlights NPS's c...

  4. Radiating Frohlich system as a model of cellular electromagnetism

    Czech Academy of Sciences Publication Activity Database

    Šrobár, Fedor

    2015-01-01

    Roč. 34, č. 4 (2015), s. 355-360. ISSN 1536-8378 Institutional support: RVO:67985882 Keywords : electromagnetic radiation * Fröhlich systems * cell physiology Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering Impact factor: 1.194, year: 2014

  5. Four-Way Relaying in Wireless Cellular Systems

    DEFF Research Database (Denmark)

    Liu, Huaping; Popovski, Petar; De Carvalho, Elisabeth; Zhao, Yuping; Sun, Fan

    2013-01-01

    Two-way relaying in wireless systems has initiated a large research effort during the past few years. Nevertheless, it represents only a specific traffic pattern and it is of interest to investigate other traffic patterns where such a simultaneous processing of information flows can bring...

  6. 32 CFR 37.1025 - Must I report information to the Defense Assistance Awards Data System?

    Science.gov (United States)

    2010-07-01

    ... required by 31 U.S.C. 6101-6106 (see 32 CFR part 21, subpart E). ... 32 National Defense 1 2010-07-01 2010-07-01 false Must I report information to the Defense... Award Reporting Information About the Award § 37.1025 Must I report information to the...

  7. Moving Target Defense

    CERN Document Server

    Jajodia, Sushil; Swarup, Vipin; Wang, Cliff; Wang, X Sean

    2011-01-01

    Moving Target Defense: Creating Asymmetric Uncertainty for Cyber Threats was developed by a group of leading researchers. It describes the fundamental challenges facing the research community and identifies new promising solution paths. Moving Target Defense which is motivated by the asymmetric costs borne by cyber defenders takes an advantage afforded to attackers and reverses it to advantage defenders. Moving Target Defense is enabled by technical trends in recent years, including virtualization and workload migration on commodity systems, widespread and redundant network connectivity, instr

  8. Iterative Cellular Screening System for Nanoparticle Safety Testing

    OpenAIRE

    Franziska Sambale; Frank Stahl; Ferdinand Rüdinger; Dror Seliktar; Cornelia Kasper; Detlef Bahnemann; Thomas Scheper

    2015-01-01

    Nanoparticles have the potential to exhibit risks to human beings and to the environment; due to the wide applications of nanoproducts, extensive risk management must not be neglected. Therefore, we have constructed a cell-based, iterative screening system to examine a variety of nanoproducts concerning their toxicity during development. The sensitivity and application of various cell-based methods were discussed and proven by applying the screening to two different nanoparticles: zinc oxide ...

  9. The cellular composition of the human immune system is shaped by age and cohabitation.

    Science.gov (United States)

    Carr, Edward J; Dooley, James; Garcia-Perez, Josselyn E; Lagou, Vasiliki; Lee, James C; Wouters, Carine; Meyts, Isabelle; Goris, An; Boeckxstaens, Guy; Linterman, Michelle A; Liston, Adrian

    2016-04-01

    Detailed population-level description of the human immune system has recently become achievable. We used a 'systems-level' approach to establish a resource of cellular immune profiles of 670 healthy individuals. We report a high level of interindividual variation, with low longitudinal variation, at the level of cellular subset composition of the immune system. Despite the profound effects of antigen exposure on individual antigen-specific clones, the cellular subset structure proved highly elastic, with transient vaccination-induced changes followed by a return to the individual's unique baseline. Notably, the largest influence on immunological variation identified was cohabitation, with 50% less immunological variation between individuals who share an environment (as parents) than between people in the wider population. These results identify local environmental conditions as a key factor in shaping the human immune system. PMID:26878114

  10. Analytical Results to Improve the Capacity of A Cellular System In Frequency Selective Rayleigh Fading Channel

    Directory of Open Access Journals (Sweden)

    Pravindra Kumar

    2010-10-01

    Full Text Available One of the biggest draw back of wireless environment is the limited bandwidth. However, the users sharing this limited bandwidth have been increased considerably by using SDMA technique that can enhancethe capacity of communication system. There are some techniques that can increase the capacity of the cellular system, these are- Spreading Technique, Error Control Coding Technique, Multipath Diversity Technique ( i.e. Rake Receiver, Smart Antenna Technique. In this paper we have used all these technique and examined how thecapacity of cellular system vary with varying the different parameters such as- the value of spreading factor, the number of Rake fingers, the number of interfering cells, value of directivity of Adaptive Antenna at base station. In the results we find that the capacity of a cellular system is varying with these parameters.

  11. Aggregatibacter actinomycetemcomitans, a potent immunoregulator of the periodontal host defense system and alveolar bone homeostasis.

    Science.gov (United States)

    Herbert, B A; Novince, C M; Kirkwood, K L

    2016-06-01

    Aggregatibacter actinomycetemcomitans is a perio-pathogenic bacteria that has long been associated with localized aggressive periodontitis. The mechanisms of its pathogenicity have been studied in humans and preclinical experimental models. Although different serotypes of A. actinomycetemcomitans have differential virulence factor expression, A. actinomycetemcomitans cytolethal distending toxin (CDT), leukotoxin, and lipopolysaccharide (LPS) have been most extensively studied in the context of modulating the host immune response. Following colonization and attachment in the oral cavity, A. actinomycetemcomitans employs CDT, leukotoxin, and LPS to evade host innate defense mechanisms and drive a pathophysiologic inflammatory response. This supra-physiologic immune response state perturbs normal periodontal tissue remodeling/turnover and ultimately has catabolic effects on periodontal tissue homeostasis. In this review, we have divided the host response into two systems: non-hematopoietic and hematopoietic. Non-hematopoietic barriers include epithelium and fibroblasts that initiate the innate immune host response. The hematopoietic system contains lymphoid and myeloid-derived cell lineages that are responsible for expanding the immune response and driving the pathophysiologic inflammatory state in the local periodontal microenvironment. Effector systems and signaling transduction pathways activated and utilized in response to A. actinomycetemcomitans will be discussed to further delineate immune cell mechanisms during A. actinomycetemcomitans infection. Finally, we will discuss the osteo-immunomodulatory effects induced by A. actinomycetemcomitans and dissect the catabolic disruption of balanced osteoclast-osteoblast-mediated bone remodeling, which subsequently leads to net alveolar bone loss. PMID:26197893

  12. Design of mobile telemedicine systems using GSM and IS-54 cellular telephone standards.

    Science.gov (United States)

    Istepanian, R H; Woodward, B; Gorilas, E; Balos, P A

    1998-01-01

    This paper presents an overview of the design of mobile telemedical systems using cellular telephone channels. A mobile telemedicine communication system was studied using both the GSM and the IS-54 standards, which are the most widely used commercial cellular telephone systems in Europe and North America, respectively. A simulation using a photoplethesmography signal showed successful transmission of data with bit error rates of less than 10(-7) at the receiver for the IS-54 standard and less than 10(-5) for the GSM standard, depending on the mobile channel conditions used. PMID:9640747

  13. Nervous system disease associated with dominant cellular radiosensitivity

    International Nuclear Information System (INIS)

    Ionizing radiation sensitivity has been demonstrated in the following neurological diseases: sporadic and familial Alzheimer's disease, familial non-specific dementia, amyotrophic lateral sclerosis and Parkinsonism dementia of Guam, Huntington's disease, multiple sclerosis. Family studies in many cases give data consistent with dominant genetics, as does cell fusion analysis in the one disease so studied. In no case was there an absolute association between radiosensitivity and a given neurological disease. It is proposed that the underlying mutations are in genes controlling facets of nervous or immune system differentiation and development. 15 references, 2 tables

  14. Method for analyzing signaling networks in complex cellular systems.

    Science.gov (United States)

    Plavec, Ivan; Sirenko, Oksana; Privat, Sylvie; Wang, Yuker; Dajee, Maya; Melrose, Jennifer; Nakao, Brian; Hytopoulos, Evangelos; Berg, Ellen L; Butcher, Eugene C

    2004-02-01

    Now that the human genome has been sequenced, the challenge of assigning function to human genes has become acute. Existing approaches using microarrays or proteomics frequently generate very large volumes of data not directly related to biological function, making interpretation difficult. Here, we describe a technique for integrative systems biology in which: (i) primary cells are cultured under biologically meaningful conditions; (ii) a limited number of biologically meaningful readouts are measured; and (iii) the results obtained under several different conditions are combined for analysis. Studies of human endothelial cells overexpressing different signaling molecules under multiple inflammatory conditions show that this system can capture a remarkable range of functions by a relatively small number of simple measurements. In particular, measurement of seven different protein levels by ELISA under four different conditions is capable of reconstructing pathway associations of 25 different proteins representing four known signaling pathways, implicating additional participants in the NF-kappaBorRAS/mitogen-activated protein kinase pathways and defining additional interactions between these pathways. PMID:14745015

  15. Cellular automata

    CERN Document Server

    Codd, E F

    1968-01-01

    Cellular Automata presents the fundamental principles of homogeneous cellular systems. This book discusses the possibility of biochemical computers with self-reproducing capability.Organized into eight chapters, this book begins with an overview of some theorems dealing with conditions under which universal computation and construction can be exhibited in cellular spaces. This text then presents a design for a machine embedded in a cellular space or a machine that can compute all computable functions and construct a replica of itself in any accessible and sufficiently large region of t

  16. Multicompartmentalized polymeric systems: towards biomimetic cellular structure and function.

    Science.gov (United States)

    Marguet, Maïté; Bonduelle, Colin; Lecommandoux, Sébastien

    2013-01-21

    The cell is certainly one of the most complex and exciting systems in Nature that scientists are still trying to fully understand. Such a challenge pushes material scientists to seek to reproduce its perfection by building biomimetic materials with high-added value and previously unmatched properties. Thanks to their versatility, their robustness and the current state of polymer chemistry science, we believe polymer-based materials to constitute or represent ideal candidates when addressing the challenge of biomimicry, which defines the focus of this review. The first step consists in mimicking the structure of the cell: its inner compartments, the organelles, with a multicompartmentalized structure, and the rest, i.e. the cytoplasm minus the organelles (mainly cytoskeleton/cytosol) with gels or particular solutions (highly concentrated for example) in one compartment, and finally the combination of both. Achieving this first structural step enables us to considerably widen the gap of possibilities in drug delivery systems. Another powerful property of the cell lies in its metabolic function. The second step is therefore to achieve enzymatic reactions in a compartment, as occurs in the organelles, in a highly controlled, selective and efficient manner. We classify the most exciting polymersome nanoreactors reported in our opinion into two different subsections, depending on their very final concept or purpose of design. We also highlight in a thorough table the experimental sections crucial to such work. Finally, after achieving control over these prerequisites, scientists are able to combine them and push the frontiers of biomimicry further: from cell structure mimics towards a controlled biofunctionality. Such a biomimetic approach in material design and the future research it will stimulate, are believed to bring considerable enrichments to the fields of drug delivery, (bio)sensors, (bio)catalysis and (bio)technology. PMID:23073077

  17. Design mobile satellite system architecture as an integral part of the cellular access digital network

    Science.gov (United States)

    Chien, E. S. K.; Marinho, J. A.; Russell, J. E., Sr.

    1988-01-01

    The Cellular Access Digital Network (CADN) is the access vehicle through which cellular technology is brought into the mainstream of the evolving integrated telecommunications network. Beyond the integrated end-to-end digital access and per call network services provisioning of the Integrated Services Digital Network (ISDN), the CADN engenders the added capability of mobility freedom via wireless access. One key element of the CADN network architecture is the standard user to network interface that is independent of RF transmission technology. Since the Mobile Satellite System (MSS) is envisioned to not only complement but also enhance the capabilities of the terrestrial cellular telecommunications network, compatibility and interoperability between terrestrial cellular and mobile satellite systems are vitally important to provide an integrated moving telecommunications network of the future. From a network standpoint, there exist very strong commonalities between the terrestrial cellular system and the mobile satellite system. Therefore, the MSS architecture should be designed as an integral part of the CADN. This paper describes the concept of the CADN, the functional architecture of the MSS, and the user-network interface signaling protocols.

  18. Molecular and cellular designs of insect taste receptor system

    Directory of Open Access Journals (Sweden)

    Kunio Isono

    2010-06-01

    Full Text Available The insect gustatory receptors (GRs are members of a large G-protein coupled receptor family distantly related to the insect olfactory receptors. They are phylogenetically different from taste receptors of most other animals. GRs are often coexpressed with other GRs in single receptor neurons. Taste receptors other than GRs are also expressed in some neurons. Recent molecular studies in the fruitfly Drosophila revealed that the insect taste receptor system not only covers a wide ligand spectrum of sugars, bitter substances or salts that are common to mammals but also includes reception of pheromone and somatosensory stimulants. However, the central mechanism to perceive and discriminate taste information is not yet elucidated. Analysis of the primary projection of taste neurons to the brain shows that the projection profiles depend basically on the peripheral locations of the neurons as well as the GRs that they express. These results suggest that both peripheral and central design principles of insect taste perception are different from those of olfactory perception.

  19. Analysis of the NATO Fleet Air-Defense System%北约舰队防空体系分析

    Institute of Scientific and Technical Information of China (English)

    齐强; 王瑞麒

    2001-01-01

    介绍了以美国为首的北约舰队防空体系的构成、任务和主要防御对象。对北约舰队防空体系的最新发展动向进行了分析。针对目前北约舰队防空体系中的薄弱环节,提出了突防的具体构想。%This paper introduces the construction, tasks ,targets and analyzed the future developing tendency of the fleet air-defense system of NATO led by U.S.. According to the weak links of the defense system,this paper presents a idea to break through the defense net.

  20. A miniature mimic of host defense peptides with systemic antibacterial efficacy

    International Nuclear Information System (INIS)

    Oligomers of acylated lysines (OAKs) are synthetic mimics of host defense peptides (HDPs) with promising antimicrobial properties. Here we challenged the OAK concept for its ability to generate both systemically efficient and economically viable lead compounds for fighting multidrug-resistant bacteria. We describe the design and characterization of a miniature OAK composed of only 3 lysyls and 2 acyls (designated C12(ω7)K-β12) that preferentially targets gram-positive species by a bacteriostatic mode of action. To gain insight into the mechanism of action, we examined the interaction of OAK with various potential targets, including phospholipid bilayers, using surface plasmon resonance, and Langmuir monolayers, using insertion assays, epifluorescence microscopy, and grazing incidence X-ray diffraction, in a complementary manner. Collectively, the data support the notion that C12(ω7)K-β12 damages the plasma-membrane architecture similarly to HDPs, that is, following a near-classic 2-step interaction including high-affinity electrostatic adhesion and a subsequent shallow insertion that was limited to the phospholipid head group region. Notably, preliminary acute toxicity and efficacy studies performed with mouse models of infection have consolidated the potential of OAK for treating bacterial infections, including systemic treatments of methicillin-resistant Staphylococcus aureus. Such simple yet robust chemicals might be useful for various antibacterial applications while circumventing potential adverse effects associated with cytolytic compounds.

  1. Teaching Clinical (and Nonclinical) Psychology through Applications to the Legal System: Violence Risk Assessment and the Insanity Defense

    Science.gov (United States)

    Costanzo, Marina L.; Costanzo, Mark A.

    2013-01-01

    The prediction of dangerousness and the insanity defense are two areas where psychologists provide research-based expertise to the courts. Teachers of psychology can use these topics to capture the attention of students and to show how psychological research and theory can inform and influence the legal system. Specifically, teachers can use the…

  2. Joint-Service Integration: An Organizational Culture Study of the United States Department of Defense Voluntary Education System

    Science.gov (United States)

    Benson, Martin K.

    2010-01-01

    The purpose of the descriptive case study with a multiple case framework was to (a) describe the organizational cultures of education programs and leaders in the United States (U.S.) Department of Defense (DoD) voluntary education system on Oahu, Hawaii; (b) determine if an overlapping common organizational culture exists; and (c) assess the…

  3. Application of spectral hole burning to the study of in vitro cellular systems

    Energy Technology Data Exchange (ETDEWEB)

    Milanovich, Nebojsa

    1999-11-08

    Chapter 1 of this thesis describes the various stages of tumor development and a multitude of diagnostic techniques used to detect cancer. Chapter 2 gives an overview of the aspects of hole burning spectroscopy important for its application to the study of cellular systems. Chapter 3 gives general descriptions of cellular organelles, structures, and physical properties that can serve as possible markers for the differentiation of normal and cancerous cells. Also described in Chapter 3 are the principles of cryobiology important for low temperature spectroscopy of cells, characterization of MCF-10F (normal) and MCF-7 (cancer) cells lines which will serve as model systems, and cellular characteristics of aluminum phthalocyanine tetrasulfonate (APT), which was used as the test probe. Chapters 4 and 5 are previously published papers by the author pertaining to the results obtained from the application of hole burning to the study of cellular systems. Chapter 4 presents the first results obtained by spectral hole burning of cellular systems and Chapter 5 gives results for the differentiation of MCF-10F and MCF-7 cells stained with APT by an external applied electric (Stark) field. A general conclusion is presented in Chapter 6. Appendices A and B provide additional characterization of the cell/probe model systems. Appendix A describes the uptake and subcellular distribution of APT in MCF-10F and MCF-7 cells and Appendix B compares the hole burning characteristics of APT in cells when the cells are in suspension and when they are examined while adhering to a glass coverslip. Appendix C presents preliminary results for a novel probe molecule, referred to as a molecular thumbtack, designed by the authors for use in future hole burning applications to cellular systems.

  4. The biocontrol endophytic bacterium Pseudomonas fluorescens PICF7 induces systemic defense responses in aerial tissues upon colonization of olive roots

    Directory of Open Access Journals (Sweden)

    Carmen eGómez-Lama Cabanás

    2014-09-01

    Full Text Available Pseudomonas fluorescens PICF7, a native olive root endophyte and effective biocontrol agent (BCA against Verticillium wilt of olive, is able to trigger a broad range of defense responses in root tissues of this woody plant. In order to elucidate whether strain PICF7 also induces systemic defense responses in above-ground organs, aerial tissues of olive plants grown under non-gnotobiotic conditions were collected at different time points after root bacterization with this endophytic BCA. A suppression subtractive hybridization (SSH cDNA library, enriched in up-regulated genes, was generated. This strategy enabled the identification of 376 ESTs (99 contigs and 277 singlets, many of them related to response to different stresses. Five ESTs, involved in defense responses, were selected to carry out time-course quantitative real-time PCR (qRT-PCR experiments aiming to: (i validate the induction of these genes, and (ii shed light on their expression pattern along time (from 1 to 15 days. Induction of olive genes potentially coding for lypoxigenase 2, catalase, 1-aminocyclopropane-1-carboxylate oxidase and phenylananine ammonia-lyase was thus confirmed at some time points. Computational analysis also revealed that different transcription factors were up-regulated in olive aerial tissues (i.e. jerf, bHLH, WRKYs, as previously reported for roots. Results confirmed that root colonization by this endophytic bacterium does not only trigger defense responses in this organ but also mount a wide array of systemic defense responses in distant tissues (stems, leaves. This sheds light on how olive plants respond to the ‘non-hostile’ colonization by a bacterial endophyte and how induced defense response can contribute to the biocontrol activity of strain PICF7.

  5. Bayesian approaches to reverse engineer cellular systems: a simulation study on nonlinear Gaussian networks

    Directory of Open Access Journals (Sweden)

    Ramoni Marco F

    2007-05-01

    Full Text Available Abstract Background Reverse engineering cellular networks is currently one of the most challenging problems in systems biology. Dynamic Bayesian networks (DBNs seem to be particularly suitable for inferring relationships between cellular variables from the analysis of time series measurements of mRNA or protein concentrations. As evaluating inference results on a real dataset is controversial, the use of simulated data has been proposed. However, DBN approaches that use continuous variables, thus avoiding the information loss associated with discretization, have not yet been extensively assessed, and most of the proposed approaches have dealt with linear Gaussian models. Results We propose a generalization of dynamic Gaussian networks to accommodate nonlinear dependencies between variables. As a benchmark dataset to test the new approach, we used data from a mathematical model of cell cycle control in budding yeast that realistically reproduces the complexity of a cellular system. We evaluated the ability of the networks to describe the dynamics of cellular systems and their precision in reconstructing the true underlying causal relationships between variables. We also tested the robustness of the results by analyzing the effect of noise on the data, and the impact of a different sampling time. Conclusion The results confirmed that DBNs with Gaussian models can be effectively exploited for a first level analysis of data from complex cellular systems. The inferred models are parsimonious and have a satisfying goodness of fit. Furthermore, the networks not only offer a phenomenological description of the dynamics of cellular systems, but are also able to suggest hypotheses concerning the causal interactions between variables. The proposed nonlinear generalization of Gaussian models yielded models characterized by a slightly lower goodness of fit than the linear model, but a better ability to recover the true underlying connections between

  6. Influenza Viral Manipulation of Sphingolipid Metabolism and Signaling to Modulate Host Defense System

    Directory of Open Access Journals (Sweden)

    Madhuvanthi Vijayan

    2014-01-01

    Full Text Available Viruses attempt to create a distinctive cellular environment to favor viral replication and spread. Recent studies uncovered new functions of the sphingolipid signaling/metabolism during pathogenic virus infections. While sphingolipids such as sphingomyelin and ceramide were reported to influence the entry step of several viruses, sphingolipid-metabolizing enzymes could directly alter viral replication processes. Influenza virus was shown to increase the level of sphingosine kinase (SK 1 to promote virus propagation. The mechanism involves regulation of intracellular signaling pathways, leading to the amplification of influenza viral RNA synthesis and nuclear export of viral ribonucleoprotein (RNP complex. However, bovine viral diarrhea virus inhibits SK1 to enhance the efficacy of virus replication, demonstrating the presence of virus-specific strategies for modulation of the sphingolipid system. Therefore, investigating the sphingolipid metabolism and signaling in the context of virus replication could help us design innovative therapeutic approaches to improve human health.

  7. Colorado potato beetle manipulates plant defenses in local and systemic leaves

    OpenAIRE

    Chung, Seung Ho; Rosa, Cristina; Hoover, Kelli; Luthe, Dawn S; Felton, Gary W.

    2013-01-01

    Herbivore microbial associates can affect diverse interactions between plants and insect herbivores. Some insect symbionts enable herbivores to expand host plant range or to facilitate host plant use by modifying plant physiology. However, little attention has been paid to the role of herbivore-associated microbes in manipulating plant defenses. We have recently shown that Colorado potato beetle secrete the symbiotic bacteria to suppress plant defenses. The bacteria in oral secretions from th...

  8. Brassinosteroids Denigrate the Seasonal Stress through Antioxidant Defense System in Seedlings of Brassica juncea L.

    Directory of Open Access Journals (Sweden)

    Sandeep Kumar

    2014-05-01

    Full Text Available The present work has been undertaken to study the effect of exogenously application of 24-epiBL and 28-homoBL on soluble protein, proline contents and antioxidant defense system of Brassica juncea L. RLM 619 under the influence of seasonal stress. It was observed that 24-epiBL and 28-homoBL treatment enhance the soluble protein, dry weight and shoot length of B. juncea seedlings under seasonal stress. If seeds treated with the different concentrations (10-6, 10-8 and 10-10 M of 24-epiBL and 28-homoBL revealed batter growth, protein and proline contents as compare to untreated seedlings. Similarly the activities of antioxidant enzymes SOD, CAT, APOX, DHAR, PPO and Auxinases were enhanced by the application of different concentration of both brassinosteroids, whereas MDA content was decrease with both brassinosteroids treatments. Then we have concluded that both brassinolides have the seasonal stress ameliorative properties in B. juncea seedlings grown under the influence of seasonal stress. This study culminates to the role of brassinolides as an anti-stress property for protection of plant from various types of stresses.

  9. Effect of 3-bromopyruvic acid on human erythrocyte antioxidant defense system.

    Science.gov (United States)

    Sadowska-Bartosz, Izabela; Bartosz, Grzegorz

    2013-12-01

    3-Bromopyruvate (3-BP) is a promising compound for anticancer therapy, its main mode of action being the inhibition of glycolytic enzymes, but this compound also induces oxidative stress. This study aimed at characterisation of the effect of 3-BP on the antioxidant defense system of erythrocytes. Suspensions of erythrocytes in PBS containing 5 mM glucose were treated with different concentration of 3-BP at 37°C for 1 h. Activities of antioxidant enzymes were estimated by standard colorimetric methods. The antioxidant capacity of erythrocytes was estimated using the 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS(•+)) decolorisation assay and ferricyanide reduction. The content of reduced and oxidized glutathione was estimated fluorimetrically with o-phtalaldehyde. 3-BP did not affect the integrity of the erythrocyte membrane (lack of changes in the osmotic fragility). However, it induced oxidative stress in erythrocytes, as evidenced by the decrease in the content of acid-soluble thiols and reduced glutathione (GSH). Superoxide dismutase (SOD) and glutathione S-transferase (GST) activities were significantly decreased. 3-BP also decreased the transmembrane reduction of ferricyanide. Thus induction of oxidative stress in erythrocytes by 3-BP is due to depletion of glutathione and inhibition of antioxidant enzymes. PMID:23881849

  10. Responses of antioxidant defense system of Lespedeza davurica to enhanced UV-B radiation

    International Nuclear Information System (INIS)

    The objective of this study was to investigate the effects of different UV-B radiation intensity (CK, T1, T2) on antioxidant defense system and other related indicators of Lespedeza davurica (Laxm.) Schindl. Malonaldehyde (MDA), ascorbic acid (AsA) and carotenoid (Car) contents, as well as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) activities of leaves from Lespedeza davurica under different UV-B radiation intensity were investigated. Samples were collected once every three days. The UV-B treatment was continued 15 days. Result indicated that SOD and POD activities decreased, APX and POD activities increased with UV-B radiation enhanced during the whole treatment time. SOD, POD and CAT activities decreased with UV-B radiation intensity increasing. APX activity increased during the first 9-day treatment with radiation intensity increasing then decreased with radiation intensity increasing. UV-B radiation increased AsA content, decreased Car content. Both AsA and Car contents decreased with radiation intensity increasing when compared with control. O2- and MDA increased with radiation intensity increasing. All other tested indicators increased except SOD and POD activity as well as AsA content decreased after UV-B radiation treatment. Comprehensive evaluation of subordinate function showed that UV-B radiation reduced the antioxidant capacity of Lespedeza davurica, and the antioxidant capacity decreased with UV-B radiation intensity increasing. (author)

  11. Imbalanced free radicals and antioxidant defense systems in schizophrenia: A comparative study

    Institute of Scientific and Technical Information of China (English)

    LI Hui-chun; CHEN Qiao-zhen; MA Ying; ZHOU Jun-fu

    2006-01-01

    Objective: To examine changes of blood oxidative-antiovidative level in schizophrenic patients and its relationship with clinical symptoms. Methods: Forty-six Chinese patients met DSM-Ⅳ (Diagnostic and Statistical Manual of Mental Disorders-Ⅳ) criteria for schizophrenia and fifty age- and sex-matched healthy controls were enrolled in the present study. Baseline psychiatric symptom severity was assessed with brief psychiatric rating scale, positive and negative syndrome scale on the blood draw day. Fresh blood samples were collected to measure levels of nitric oxide and lipid peroxide in plasma as well as activities of superoxide dismutase, catalase and glutathione peroxidase in red blood cells by spectrophotometric assays simultaneously. Results:Comparison of the biochemical parameters indicated that the level of nitric oxide and lipid peroxide increased in patient group,which represented a positive correlation with positive scale scores; while the activities of three critical enzymes decreased and showed a negative linear correlation. Conclusion: This study showed that there are dysregulation of free radical metabolism and poor activities of the antioxidant defense systems in schizophrenic patients. Excess free radicals formation may play a critical role in the etiology of schizophrenia. Using antioxidants might be an effective therapeutic approach to partially alleviate or prevent the symptoms of schizophrenia.

  12. Plant natriuretic peptides: Systemic regulators of plant homeostasis and defense that can affect cardiomyoblasts

    KAUST Repository

    Gehring, Christoph A

    2010-09-01

    Immunologic evidence has suggested the presence of biologically active natriuretic peptide (NPs) hormones in plants because antiatrial NP antibodies affinity purify biologically active plant NPs (PNP). In the model plant, an Arabidopsis thaliana PNP (AtPNP-A) has been identified and characterized. AtPNP-A belongs to a novel class of molecules that share some similarity with the cell wall loosening expansins but do not contain the carbohydrate-binding wall anchor thus suggesting that PNPs and atrial natriuretic peptides are heterologs. AtPNP-A acts systemically, and this is consistent with its localization in the apoplastic extracellular space and the conductive tissue. Furthermore, AtPNP-A signals via the second messenger cyclic guanosine 3′,5′-monophosphate and modulates ion and water transport and homeostasis. It also plays a critical role in host defense against pathogens. AtPNP-A can be classified as novel paracrine plant hormone because it is secreted into the apoplastic space in response to stress and can enhance its own expression. Interestingly, purified recombinant PNP induces apo-ptosis in a dose-dependent manner and was most effective on cardiac myoblast cell lines. Because PNP is mimicking the effect of ANP in some instances, PNP may prove to provide useful leads for development of novel therapeutic NPs. Copyright © 2013 by The American Federation for Medical Research.

  13. Contemporary strategic management approach in the defense system by U.S. methodology concept

    Directory of Open Access Journals (Sweden)

    Veselin I. Mrdak

    2013-12-01

    Full Text Available The paper wants to emphasize the importance of contemporary strategic management approachs in the defense system. Displays the work of American author Leslie Lewis and C. Robert Roll "Strategy-to- tasks: a methodology for resource allocation and management". It were used by parts of the discussion above, in order to describe the strategy-to-tasks methodology for planning, programming and budgeting system (PPBS. In developed Western countries adopted some elements of strategic management to determine and monitor the execution of strategic plans in the defense system. This trend is spreading to other countries as a result of the necessity of obeying certain methodological and technological achievements. The uncritical acceptance of a strategic management approach can cause great confusion and problems, and the terminology and scientific explanation for this trend is of great importance. Introduction PPBS is a resource management framework based on force planning concept. It was developed at the lRand Company during the late eighties and is adapted to the special needs of several DoD organization. The planning, programming and budgeting system (PPBS PPBS is DoD`ş primary system for planning and managing defense resources. It links the overall U.S. national security strategy to specific programs. It was designed to facilitate fiscally-constrained planning, programming and budgeting in terms of complete programs (i.e. forces and systems rather than through artificial budget categories. The goal is to determine forces, systems and program costs. Strategy-to-tasks and the PPBS The Strategy-To-Tasks methodology, regardless of its application, it must be consistent and supportive of each phase of the PPBS. This section describes the basics frameworks of which are adapted to resource allocation and management activities for the Special Operations Command (USSOCOM. The authors have used it during the various phases of the PPBS. The National Security

  14. A survey of COTS wireless transceiver solutions for unmanned/unattended homeland defense systems

    Science.gov (United States)

    Wurth, Timothy J.; Wells, Jeffrey S.; Trimble, Michael L.

    2004-08-01

    As the war on terrorism continues abroad, the need to maintain security at home remains a major concern. Every aspect of the security effort can benefit from rapid information transfer. Advances in Commercial-Off-the-Shelf (COTS) radios make them valuable in applications ranging from deployed sensors on unsecured borders, to organizing an emergency response team. Additionally, the reduced cost and availability of these transceivers make them a viable alternative to custom developments. As an example, COTS radios can serve as the communication element in Unattended Ground Sensor (UGS) and munitions systems. Other applications include networked sonobuoys and networked RFID tracking. An additional advantage of COTS solutions is that they are available in a small form factor. They have extremely small mechanical outlines and are easily installed in systems requiring miniature designs and light payloads such as man-pack emplaced radios for the Army"s Future Combat System (FCS), as well as missions carried out by Unmanned Aerial Vehicles (UAV). The availability of the COTS radios result in a low-cost alternative for communication links that are applicable to military and Homeland Defense projects. They provide an easily obtainable, low-cost radio alternative enabling quick design cycles to meet mission timeliness. COTS solutions eliminate months from a typical design cycle and have many features already implemented such as ad-hoc routing and encryption. This enables robust hardware to be fielded quickly when a new need arises. The focus of this paper is to identify different COTS modules that can efficiently and cost effectively be applied to these and other various applications.

  15. Multilevel Access Control in a MANET for a Defense Messaging system using Elliptic Curve Cryptography

    Directory of Open Access Journals (Sweden)

    J.Nafeesa Begum, K.Kumar, Dr.V.Sumathy

    2010-06-01

    Full Text Available The trend of the Civilian society has moved from the industrial age focus onautomation and scale towards information based on computing andcommunication. Today’s Warfare is also moving towards an information ageparadigm based on information sharing, situational awareness, and distributedpoints of intelligence, command and control. A widely-networked fighting force isbetter able to share information about tactical situations that may begeographically widespread, asymmetric, and rapidly changing. Commandersmust be able to better assess situations across broad theaters, with extensivedata, voice, and especially video feeds as strategic inputs. Thus, network-centricwarfare improves effectiveness at both the tactical "point of the spear" and in theachievement of broader strategic goals. Broadly disseminated knowledge assetsenable fighting forces that must self-synchronize, even as they physicallydisperse to address dynamic battlefield conditions. The speed of decision hasincreased and command decisions must be rapidly relayed and implemented, toimprove battlefield outcomes. Multilevel access control in a MANET for aDefense messaging system is used to have the command decisions relayed toall people who are active in the group and also to all people who have beenidentified as higher in the hierarchy instead of sending one to one messages toeach individual.. The system developed is secure, multi site and allows for globalcommunication using the inherent properties of Elliptic Curve cryptography .Elliptic Curve cryptography provides a greater security with less bit size and it isfast when compared to other schemes. The implementation suggests that it is asecure system which occupies fewer bits and can be used for low power devices.

  16. Phagocytosis, a cellular immune response in insects

    Directory of Open Access Journals (Sweden)

    C Rosales

    2011-06-01

    Full Text Available Insects like many other organisms are exposed to a wide range of infectious agents. Defense against these agents is provided by innate immune systems, which include physical barriers, humoral responses, and cellular responses. The humoral responses are characterized by the production of antimicrobial peptides, while the cellular defense responses include nodulation, encapsulation, melanization and phagocytosis. The phagocytic process, whereby cells ingest large particles, is of fundamental importance for insects’ development and survival. Phagocytic cells recognize foreign particles through a series of receptors on their cell membrane for pathogen-associated molecules. These receptors in turn initiate a series of signaling pathways that instruct the cell to ingest and eventually destroy the foreign particle. This review describes insect innate humoral and cellular immune functions with emphasis on phagocytosis. Recent advances in our understanding of the phagocytic cell types in various insect species; the receptors involved and the signaling pathways activated during phagocytosis are discussed.

  17. Modelling Cellular Processes using Membrane Systems with Peripheral and Integral Proteins

    OpenAIRE

    Cavaliere, Matteo; Sedwards, Sean

    2006-01-01

    Membrane systems were introduced as models of computation inspired by the structure and functioning of biological cells. Recently, membrane systems have also been shown to be suitable to model cellular processes. We introduce a new model called Membrane Systems with Peripheral and Integral Proteins. The model has compartments enclosed by membranes, floating objects, objects associated to the internal and external surfaces of the membranes and also objects integral to the membranes. The floati...

  18. Mimo radar waveform design for spectrum sharing with cellular systems a Matlab based approach

    CERN Document Server

    Khawar, Awais; Clancy, T Charles

    2016-01-01

    This book discusses spectrum sharing between cellular systems and radars. The book addresses a novel way to design radar waveforms that can enable spectrum sharing between radars and communication systems, without causing interference to communication systems, and at the same time achieving radar objectives of target detection, estimation, and tracking. The book includes a MATLAB-based approach, which provides reader with a way to learn, experiment, compare, and build on top of existing algorithms.

  19. Budgeting for National Defense Acquisition: assessing system linkage and the impact of transformation / by Jones, Lawrence R., and Jerry McCaffery.

    OpenAIRE

    Jones, Lawrence R.; McCaffery, Jerry; Fierstine, Kory L.

    2005-01-01

    In this article we conduct a processual analysis (Barzelay, 2003; Barzelay & Gallego, 2005) to assess evidence and test the following hypothesis: the complicated architecture and processes of national defense planning, programming, budgeting and execution and the defense acquisition decision system lead to unintended and negative consequences for defense acquisition and procurement. The purpose of this article is to identify key points of linkage weakness or failure between DoD financial mana...

  20. An integrated approach for the cell formation and layout design in cellular manufacturing systems

    NARCIS (Netherlands)

    Javadi, Babak; Jolai, Fariborz; Slomp, Jannes; Rabbani, Masoud; Tavakkoli-Moghaddam, Reza

    2013-01-01

    In this paper, a comprehensive model is presented for cell formation and layout design in cellular manufacturing systems (CMS). The proposed model incorporates an extensive coverage of important operational features and especially layout design aspects to determine optimal cell configuration and Int

  1. Angiotensin II and vasopressin are involved in the defense system against anaphylactic hypotension in anesthetized rats.

    Science.gov (United States)

    Wang, Mofei; Shibamoto, Toshishige; Kuda, Yuhichi; Sun, Lingling; Tanida, Mamoru; Kurata, Yasutaka

    2014-05-15

    Anaphylactic shock is sometimes life-threatening, but the defense system against this circulatory failure was not fully understood. Ameliorating roles of angiotensin (ANG) II and vasopressin in anaphylactic hypotension were investigated in anesthetized ovalbumin-sensitized Sprague-Dawley rats. The sensitized rats were randomly allocated to the following pretreatment groups (n=7/group): (1) control (non-pretreatment), (2) ANG II synthesis inhibitor captopril, (3) ANG II receptor antagonist losartan, and (4) V1a vasopressin receptor antagonist. Anaphylactic shock was induced by an intravenous injection of the antigen. The systemic arterial pressure (SAP), central venous pressure (CVP), portal venous pressure (PVP) and portal venous blood flow (PBF) were measured, and splanchnic vascular resistance (Rspl: (SAP-PVP)/PBF) was determined. In the control group, SAP markedly decreased, followed by a gradual recovery toward baseline. Rspl transiently decreased immediately after antigen, and then increased 1.5-fold at 15 min and thereafter. The pretreatment with either losartan, captopril or V1a receptor antagonist augmented the initial fall of SAP and attenuated the SAP recovery along with augmentation of the late increase in Rspl. The 2-h survival rate was significantly smaller in either pretreatment group than in the control group (100%). Plasma levels of ANG II and vasopressin increased to 3.8- and 9.8-fold, respectively, at 30 min after antigen in the control group, whereas captopril pretreatment inhibited the increase in ANG II. In conclusion, inhibition of ANG II or vasopressin exacerbates anaphylaxis-induced hypotension in anesthetized rats. PMID:24650734

  2. A preliminary study on estimating extra-cellular nitrate reductase activities in estuarine systems

    OpenAIRE

    Pant H. K.

    2009-01-01

    Enzymes catalyzing ammonium (NH4+)/nitrate (NO3–) into nitrous oxide (N2O)/molecular nitrogen (N2), play critical roles in water quality management. The objective of this paper was to investigate the role of extra-cellular enzymes in cycling of nitrogen (N) in aquatic systems. It appears that N in estuaries, salt marshes, etc., does not stay long enough to be available for uptake, thus, creating N limited conditions. This study showed that indigenous extra-cellular nitrate reductase along wit...

  3. Impact of Underlaid Multi-antenna D2D on Cellular Downlink in Massive MIMO Systems

    OpenAIRE

    Agarwal, Amit; Mukherjee, Sudarshan; Mohammed, Saif Khan

    2015-01-01

    In this paper, we consider a massive multiple-input multiple-output (MIMO) downlink system underlaid with a network of multi-antenna D2D user equipments (UEs). Each D2D transmitter (Tx) uses all its antennas to beamform information towards its desired D2D receiver, which uses only a single antenna for reception. While beamforming at the D2D Tx reduces D2D interference to the neighbouring cellular UEs (CUEs), the cellular-to-D2D interference is also negligible due to highly directional beamfor...

  4. Immune multi-agent model using vaccine for cooperative air-defense system of systems for surface warship formation based on danger theory

    Institute of Scientific and Technical Information of China (English)

    Jun Wang; Xiaozhe Zhao; Beiping Xu; Wei Wang; Zhiyong Niu

    2013-01-01

    Aiming at the problem on cooperative air-defense of surface warship formation, this paper maps the cooperative air-defense system of systems (SoS) for surface warship formation (CASoSSWF) to the biological immune system (BIS) according to the similarity of the defense mechanism and characteristics be-tween the CASoSSWF and the BIS, and then designs the mo-dels of components and the architecture for a monitoring agent, a regulating agent, a kil er agent, a pre-warning agent and a com-municating agent by making use of the theories and methods of the artificial immune system, the multi-agent system (MAS), the vaccine and the danger theory (DT). Moreover a new immune multi-agent model using vaccine based on DT (IMMUVBDT) for the cooperative air-defense SoS is advanced. The immune response and immune mechanism of the CASoSSWF are analyzed. The model has a capability of memory, evolution, commendable dy-namic environment adaptability and self-learning, and embodies adequately the cooperative air-defense mechanism for the CA-SoSSWF. Therefore it shows a novel idea for the CASoSSWF which can provide conception models for a surface warship forma-tion operation simulation system.

  5. REVIEWS OF TOPICAL PROBLEMS: Study of spatially extended dynamical systems using probabilistic cellular automata

    Science.gov (United States)

    Vanag, Vladimir K.

    1999-05-01

    Spatially extended dynamical systems are ubiquitous and include such things as insect and animal populations; complex chemical, technological, and geochemical processes; humanity itself, and much more. It is clearly desirable to have a certain universal tool with which the highly complex behaviour of nonlinear dynamical systems can be analyzed and modelled. For this purpose, cellular automata seem to be good candidates. In the present review, emphasis is placed on the possibilities that various types of probabilistic cellular automata (PCA), such as DSMC (direct simulation Monte Carlo) and LGCA (lattice-gas cellular automata), offer. The methods are primarily designed for modelling spatially extended dynamical systems with inner fluctuations accounted for. For the Willamowskii-Roessler and Oregonator models, PCA applications to the following problems are illustrated: the effect of fluctuations on the dynamics of nonlinear systems; Turing structure formation; the effect of hydrodynamic modes on the behaviour of nonlinear chemical systems (stirring effects); bifurcation changes in the dynamical regimes of complex systems with restricted geometry or low spatial dimension; and the description of chemical systems in microemulsions.

  6. Cooperative joint precoding in a downlink cellular system with shared relay: Design and performance evaluation

    KAUST Repository

    Kwon, JaeWoo

    2012-10-01

    In this paper, we investigate a relay enhanced cellular system, where a relay station is located in the overlap area served by two base stations. We propose cooperative joint precoding schemes for the downlink transmission of such relay enhanced cellular system to maximize the system capacity while minimizing the interference at both the relay station and the mobile stations. We formulate the optimization problems to maximize the system capacity and design the multiuser precoding vectors at each base station and the relay station. We quantify the ergodic rate performance of the proposed multiuser precoding schemes through statistical analysis. The extensively derived ergodic expressions will facilitate the accurate performance evaluation of the proposed transmission schemes. Numerical results show that the proposed schemes can effectively cancel the interference and improve the sum rate and the outage performance for cell edge users. © 2002-2012 IEEE.

  7. Broad specificity AhpC-like peroxiredoxin and its thioredoxin reductant in the sparse antioxidant defense system of Treponema pallidum

    Science.gov (United States)

    Parsonage, Derek; Desrosiers, Daniel C.; Hazlett, Karsten R. O.; Sun, Yongcheng; Nelson, Kimberly J.; Cox, David L.; Radolf, Justin D.; Poole, Leslie B.

    2010-01-01

    Little is known about the mechanisms by which Treponema pallidum (Tp), the causative agent of syphilis, copes with oxidative stress as it establishes persistent infection within its obligate human host. The Tp genomic sequence indicates that the bacterium’s antioxidant defenses do not include glutathione and are limited to just a few proteins, with only one, TP0509, offering direct defense against peroxides. Although this Tp peroxiredoxin (Prx) closely resembles AhpC-like Prxs, Tp lacks AhpF, the typical reductant for such enzymes. Functionally, TpAhpC resembles largely eukaryotic, nonAhpC typical 2-Cys Prx proteins in using thioredoxin (Trx, TP0919) as an efficient electron donor and exhibiting broad specificity toward hydroperoxide substrates. Unlike many of the eukaryotic Prxs, however, TpAhpC is relatively resistant to inactivation during turnover with hydroperoxide substrates. As is often observed in typical 2-Cys Prxs, TpAhpC undergoes redox-sensitive oligomer formation. Quantitative immunoblotting revealed that TpTrx and TpAhpC are present at very high levels (over 100 and 300 μM, respectively) in treponemes infecting rabbit testes; their redox potentials, at -242 ± 1 and -192 ± 2 mV, respectively, are consistent with the role of TpTrx as the cellular reductant of TpAhpC. Transcriptional analysis of select antioxidant genes confirmed the presence of high mRNA levels for ahpC and trx which diminish greatly when spirochetes replicate under in vitro growth conditions. Thus, T. pallidum has evolved an extraordinarily robust, broad-spectrum AhpC as its sole mechanism for peroxide defense to combat this significant threat to treponemal growth and survival during infection. PMID:20304799

  8. Defense remote-handled transuranic waste implementation plan: Transuranic Waste Program System Integration Office

    International Nuclear Information System (INIS)

    This document presents a detailed schedule for the implementation of the strategy for managing defense remote-handled (RH) transuranic (TRU) waste. The baseline management strategy was defined in the Defense Remote-Handled Transuranic Waste Cost/Schedule Optimization Study and is summarized in this document. Also included are revised RH TRU waste inventory projections, current site management plans, a list of key decision points and milestones, and a discussion of uncertainties associated with management of RH TRU waste. The plans are summarized in a detailed schedule diagram and in an RH TRU waste work off diagram. 9 refs., 5 figs., 4 tabs

  9. Role of Multi Agent System for Qos Guarantee in Cellular Networks

    OpenAIRE

    Nupur Giri; Shrikant Bodhe

    2012-01-01

    The paper highlights the benefits of Multi Agent System for maintaining QoS in cellular network by evaluating various multi agent based call admission control strategies for different traffic conditions. It establishes the effect of degree of distribution of agents on system performance by comparing the two service architectures, namely centralized and distributed, for reactivity, responsiveness, utilization of resources, communication overhead, sustainability, scalability, robustness and mo...

  10. Cellular and subcellular localization of protein I in the peripheral nervous system.

    OpenAIRE

    Fried, G.; Nestler, E J; De Camilli, P; Stjärne, L; Olson, L; Lundberg, J M; Hökfelt, T; Ouimet, C C; Greengard, P

    1982-01-01

    The cellular and subcellular distribution of protein I, a major brain phosphoprotein, has been studied in the peripheral nervous system. The levels of protein I in various peripheral nerves and innervated peripheral tissues were determined by radioimmunoassay and radioimmunolabeling of polyacrylamide gels. The results indicated tha protein I is present throughout the peripheral nervous system. Denervation studies of adrenal medulla and iris suggested that the protein I contained in peripheral...

  11. Phase transition in the economically modeled growth of a cellular nervous system

    OpenAIRE

    Nicosia V.; Vertes P.E.; Schafer W.R.; Latora V.; Bullmore E.T.

    2013-01-01

    Spatially embedded complex networks, such as nervous systems, the Internet, and transportation networks, generally have nontrivial topological patterns of connections combined with nearly minimal wiring costs. However, the growth rules shaping these economical tradeoffs between cost and topology are not well understood. Here, we study the cellular nervous system of the nematode worm Caenorhabditis elegans, together with information on the birth times of neurons and on their spatial locations....

  12. Development Of An Agent Based Simulation For The Cellular Transport System And Scenario Based Performance Analysis

    Directory of Open Access Journals (Sweden)

    Elif Karakaya

    2016-01-01

    Full Text Available Intralogistics Systems, in the rapidly increasing global business environment, are becoming more complex to analyze and more challenging to manage. In order to achieve the highest possible performance of warehouse operations, demanded by today’s modern businesses, intralogistics systems should be agile and flexible. With this objective in mind, the Cellular Transport System (CTS was developed as an alternative intralogistics system by Fraunhofer Institute for Material Flow and Logistics. This study investigates novel intralogistics system, the CTS, and proposes an integrated simulation tool in order to systematically anticipate change drivers and take optimum measures for the CTS.

  13. ROS signaling, phytohormone signaling and toxin tolerance: defense mechanisms in Arabidopsis thaliana against Botrytis cinerea

    OpenAIRE

    Cui, Fuqiang

    2014-01-01

    To face the constant challenges from numerous pathogens in the environment, sophisticated defense systems have evolved in plants. Reactive oxygen species (ROS) and phytohormones are important cellular compounds that regulate plant defense systems to overcome biotic stresses from different pathogens. Against biotrophic pathogens, which require living host cells, hypersensitive cell death response (HR), a type of programed cell death mediated by ROS and salicylic acid (SA), is effective for imm...

  14. Compact, readily deployable reactor systems for secure power for civilian and defense applications

    International Nuclear Information System (INIS)

    The U.S. electricity system is a very complex, highly interdependent network of large power plants and long transmission lines that requires constant and precise control. Disruption can rapidly propagate through the infrastructure, causing major portions to fail, as seen in the past. Such events have been triggered by natural causes. Global terrorism raises the possibility of deliberate physical attacks on the system against power plants, transmission lines, sub-stations, etc. - or cyber attacks against computers and controls to shut it down for long periods. Domestic military bases that depend on the civilian electric grid cannot function if it goes down for extended times. Natural disasters like hurricanes Katrina and Rita have shown the need for secure emergency power. If nuclear, biological, or chemical attacks on cities were to occur, panic and evacuations would shut down much of the U.S. electric system for many months. A new reactor system, DEER (Deployable Electric Energy Reactor) can provide secure emergency power for civilian and defense needs. The DEER system is compact and quickly deployable using existing types of transport vehicles. The DEER reactors have integral gamma shields, and can be transported from their deployment site after shutdown, with very low and acceptable radiation doses to handling and transport personnel. Two DEER system concepts are described with detailed neutronic and thermal hydraulic analyses of 10 and 50 MW(e) designs for each concept. The baseline DEER-1 system uses commercial TRIGA fuel, with water coolant at standard PWR conditions. The sealed DEER-1 reactor operates for several years without refueling. After shutdown, it is removed to appropriate site for refueling or disposal. If needed, a new DEER-1 reactor can be installed at the location. The advanced DEER-2 system uses existing TRISO fuel particles in porous fuel elements with direct water cooling of the particles. After shutdown, the spent TRISO fuel particles are

  15. Cellular and molecular mechanisms of sexual differentiation in the mammalian nervous system.

    Science.gov (United States)

    Forger, Nancy G; Strahan, J Alex; Castillo-Ruiz, Alexandra

    2016-01-01

    Neuroscientists are likely to discover new sex differences in the coming years, spurred by the National Institutes of Health initiative to include both sexes in preclinical studies. This review summarizes the current state of knowledge of the cellular and molecular mechanisms underlying sex differences in the mammalian nervous system, based primarily on work in rodents. Cellular mechanisms examined include neurogenesis, migration, the differentiation of neurochemical and morphological cell phenotype, and cell death. At the molecular level we discuss evolving roles for epigenetics, sex chromosome complement, the immune system, and newly identified cell signaling pathways. We review recent findings on the role of the environment, as well as genome-wide studies with some surprising results, causing us to re-think often-used models of sexual differentiation. We end by pointing to future directions, including an increased awareness of the important contributions of tissues outside of the nervous system to sexual differentiation of the brain. PMID:26790970

  16. 75 FR 32122 - Revisions to Defense Priorities and Allocations System Regulations

    Science.gov (United States)

    2010-06-07

    ..., emergency preparedness, and recovery from natural disasters. BIS has extensive experience using its... (Emergency Preparedness) of the Robert T. Stafford Disaster Relief and Emergency Assistance Act (42 U.S.C..., Transportation, Health and Human Services, Defense, and Agriculture, will comprise the Federal Priorities...

  17. 78 FR 28756 - Defense Federal Acquisition Regulation Supplement: System for Award Management Name Changes...

    Science.gov (United States)

    2013-05-16

    ... Management (SAM) database (see FAR subpart 4.11) and does not have a CAGE code, DLA Logistics Information... by the Defense Logistics Information Service (DLIS) to identify a commercial or Government entity; or...-7011 Payments in Support of Emergencies and Contingency Operations. * * * * * (c) * * * (2) * * *...

  18. Revolutionizing the United States Army's chemical defense through the acquisition of software and software-intensive systems

    OpenAIRE

    Vogelhut, Jonas.

    1999-01-01

    This thesis presents an analysis of how the United States Army can revolutionize the passage of critical chemical defense information on the battlefield. The current process for passage of this critical information is heavily dependent on short-range and stand-alone chemical detection systems, transmitted over secure radio vertically throughout the chain of command. These factors result in inaccurate, time-lagged information reaching command decision- makers, increasing the risk of contaminat...

  19. Chronic exposure to MDMA (ecstasyinduces DNA damage, impairs functional antioxidant cellular defenses, enhances the lipid peroxidation process and alters testes histopathology in male rat

    Directory of Open Access Journals (Sweden)

    Nadia Gamal Zaki, ** Laila Abdel Kawy

    2013-04-01

    Full Text Available Background : 3,4-Methylenedioxymethamphetamine (MDMA or "ecstasy" is consumed mainly by young population. For this reason, it is especially relevant to take into consideration the effects on the reproductive system. The influence of MDMA on the fertility and reproduction of the male rat was assessed in this study. Material and methods: MDMA was administered orally at 0 mg/kg (control, 10 and 30 mg/kg to male rats for 15,30,45 consecutive days followed by 15 days withdrawal. Hormonal, biochemical, histological and testicular were evaluated in the rats. The present study aimed to investigate if daily oral administration of ecstasy at low doses(10mg for 45 days has any deleterious effects on reproductive functions of male rats. Animals were randomly divided into four groups of ten rats each, assigned as control rats, or(0mg ecstasy, rats treated with 10mg ecstasy for, (15,30,45 days, rats treated with 30mg/kg body weight ecstasy for(,15,30,45days by oral gavage. The third group(45 days was followed by 15 withdrawal period(W15. Results: The activities of superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase in testicular homogenate were decreased while the levels of lipid peroxidation increased significantly in the treated rats as compared with the corresponding group of control animals. In group 30mg, only, arachidonic acid was significantly elevated in the testicular homogenate while linoleic acid was decresed when compared to control. Testis DNA fragmentation was observed in 30mg group, but not 10.mg. It is concluded that low doses of ecstasy exposure(10 mg/Kg had moderate detrimental effects on reproductive organ system and more severe effects are likely to be observed at higher dose levels. These results indicate that ecstasy is directly toxic to primary Leydig cells, and that the decreased percentage of normal cells and the increased level of DNA damage in ecstasy -exposed Leydig cells may be responsible for

  20. A preliminary study on estimating extra-cellular nitrate reductase activities in estuarine systems

    Directory of Open Access Journals (Sweden)

    Pant H. K.

    2009-07-01

    Full Text Available Enzymes catalyzing ammonium (NH4+/nitrate (NO3– into nitrous oxide (N2O/molecular nitrogen (N2, play critical roles in water quality management. The objective of this paper was to investigate the role of extra-cellular enzymes in cycling of nitrogen (N in aquatic systems. It appears that N in estuaries, salt marshes, etc., does not stay long enough to be available for uptake, thus, creating N limited conditions. This study showed that indigenous extra-cellular nitrate reductase along with others involved in N transformations in the waters/sediments of estuarine systems can cause complete removal of NH4+ and NO3– from the waters and available NH4+ and NO3– from the sediments. These results indicate that due to high extra-cellular nitrate reductase and other enzymes associated with N transformations in sediments/waters, substantial amounts of NH4+ and NO3– can be quickly lost from the systems as N2O and/or nitric oxide (NO, in turn, creating N limited conditions in estuarine systems. Such high activities of indigenous nitrate reductase and others are useful in removing readily bioavailable N from the systems, thereby avoidance of eutrophic conditions. However, they might contribute in increasing the N2O, a potent greenhouse gas with global warming potential (GWP of 296, in the atmosphere.

  1. Consequences of Social Isolation in Rats on Their Antioxidant Defense System and Erythrocyte Deformability

    Directory of Open Access Journals (Sweden)

    Sami Aydoğan

    2009-02-01

    of the major oxidants for the erythrocyte membrane results in the decreased lipid peroxidation. Furthermore, our results reveal that the social isolation stress causes alterations in the antioxidant defense system and these alteration results in the changes in erythrocyte deformability reflecting that some tissue perfusion problems can occur with long term and repeated loneliness and especially in the early stages of the life span.

  2. A Stream Control Transmission Protocol Based OAM System of 3G Cellular Network

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    OAM (Operations, Administration and Maintenance) system is a very important component of 3G cellular network. In order to acquire overall management, fast response and steady operation, an SCTP (Stream Control Transmission Protocol) based OAM, i. e. , SOAM system was proposed. SOAM implements new characters of SCTP such as multi-stream, enforced SACK and heartbeat mechanism on its transport layer. These characters help SOAM decrease the message transmission delay and accelerate the link failure detection. Besides, a new component named SOAM agent was introduced to improve the operation efficiency of SOAM. The experimental results prove the proposed SOAM system achieves better performance on signaling transmission compared with conventional TCP based OAM system.

  3. Antimicrobial defense of the earthworm

    Czech Academy of Sciences Publication Activity Database

    Bilej, Martin; De Baetselier, P.; Beschin, A.

    2000-01-01

    Roč. 45, č. 4 (2000), s. 283-300. ISSN 0015-5632 R&D Projects: GA ČR GA310/99/1385; GA ČR GA310/00/1372 Institutional research plan: CEZ:AV0Z5020903 Keywords : cellular defense mechanisms * humoral defense mechanisms Subject RIV: EE - Microbiology, Virology Impact factor: 0.752, year: 2000

  4. The RBE of tritium-beta exposure for the induction of the adaptive response and apoptosis; cellular defense mechanisms against the biological effects of ionizing radiation

    International Nuclear Information System (INIS)

    Adaption to radiation is one of a few biological responses that has been demonstrated to occur in mammalian cells exposed to doses of ionizing radiation in the occupational exposure range. The adaptive response has been well characterized in the yeast Saccharomyces cerevisiae, although the doses required to induce the response are higher than in mammalian cells. When yeast cells are primed with sublethal doses of gamma-radiation, they subsequently undergo an adaptive response and develop resistance to radiation, heat the chemical mutagens in a time and dose dependent manner. We have used this model system to assess the relative ability of tritium-beta radiation to induce the adaptive response the examined tritium-induced radiation resistance, thermal tolerance and suppression of mutation. The results show that sublethal priming doses of tritium caused yeast cells to develop resistance to radiation, heat, and a chemical mutagen MNNG. The magnitude and kinetics of the response, per unit dose, were the same for tritium and gamma-radiation. Therefore, the relative biological effectiveness (RBE) of tritium induction of the adaptive response was about 1.0. Apoptosis is a genetically programmed cell death or cell suicide. Cells damaged by radiation can be selectively removed from the population by apoptosis and therefore eliminated as a potential cancer risk to the organism. Since we have previously shown that apoptosis is a sensitive indicator of radiation damage in human lymphocytes exposed to low doses, we have used this endpoint to investigate the potency of tritium-beta radiation. Initially, tritium was compared to X-rays for relative effectiveness at inducing apoptosis. The results showed the lymphocytes irradiated in vitro with X-rays or tritium had similar levels of apoptosis per unit dose. Therefore the relative biology effectiveness of tritium for induction of apoptosis in human lymphocytes was also about 1. In the work presented here, we have demonstrated that

  5. Redox Homeostasis and Cellular Antioxidant Systems: Crucial Players in Cancer Growth and Therapy

    Directory of Open Access Journals (Sweden)

    Barbara Marengo

    2016-01-01

    Full Text Available Reactive oxygen species (ROS and their products are components of cell signaling pathways and play important roles in cellular physiology and pathophysiology. Under physiological conditions, cells control ROS levels by the use of scavenging systems such as superoxide dismutases, peroxiredoxins, and glutathione that balance ROS generation and elimination. Under oxidative stress conditions, excessive ROS can damage cellular proteins, lipids, and DNA, leading to cell damage that may contribute to carcinogenesis. Several studies have shown that cancer cells display an adaptive response to oxidative stress by increasing expression of antioxidant enzymes and molecules. As a double-edged sword, ROS influence signaling pathways determining beneficial or detrimental outcomes in cancer therapy. In this review, we address the role of redox homeostasis in cancer growth and therapy and examine the current literature regarding the redox regulatory systems that become upregulated in cancer and their role in promoting tumor progression and resistance to chemotherapy.

  6. Green light radiation effects on free radicals inhibition in cellular and chemical systems.

    Science.gov (United States)

    Comorosan, Sorin; Polosan, Silviu; Jipa, Silviu; Popescu, Irinel; Marton, George; Ionescu, Elena; Cristache, Ligia; Badila, Dumitru; Mitrica, Radu

    2011-01-10

    Free radicals generation is inhibited through green light (GL) irradiation in cellular systems and in chemical reactions. Standard melanocyte cultures were UV-irradiated and the induced cellular reactive oxygen species (ROS) were quantified by the fluorescence technique. The same cell cultures, previously protected by a 24h GL exposure, displayed a significantly lower ROS production. A simple chemical reaction is subsequently chosen, in which the production of free radicals is well defined. Paraffin wax and mineral oil were GL irradiated during thermal degradation and the oxidation products checked by chemiluminescence [CL] and Fourier transform infrared spectra [FT-IR]. The same clear inhibition of the radical oxidation of alkanes is recorded. A quantum chemistry modeling of these results is performed and a mechanism involving a new type of Rydberg macromolecular systems with implications for biology and medicine is suggested. PMID:20934350

  7. An analytical approach to air defense: cost, effectiveness and SWOT analysis of employing fighter aircraft and modern SAM systems

    Science.gov (United States)

    Kus, Orcun; Kocaman, Ibrahim; Topcu, Yucel; Karaca, Volkan

    2012-05-01

    The problem of defending a specific airspace is among the main issues a military commander to solve. Proper protection of own airspace is crucial for mission success at the battlefield. The military doctrines of most world armed forces involve two main options of defending the airspace. One of them is utilizing formations of fighter aircraft, which is a flexible choice. The second option is deploying modern SAM (Surface to Air Missile) systems, which is more expansive. On the other hand the decision makers are to cope with miscellaneous restrictions such as the budgeting problems. This study defines air defense concept according to modern air warfare doctrine. It considers an air defense scenario over an arbitrary airspace and compares the performance and cost-effectiveness of employing fighter aircraft and SAM systems. It also presents SWOT (Strenghts - Weakness - Opportunities - Threats) analyses of air defense by fighter aircraft and by modern SAMs and tries to point out whichever option is better. We conclude that deploying SAMs has important advantages over using fighter aircraft by means of interception capacity within a given time period and is cost-effective.

  8. Improved Analysis of Co-Channel Interference in Cellular Communications Systems

    Institute of Scientific and Technical Information of China (English)

    ZHANG Zu-fan; DU Hui-ping; ZHU Wei-le

    2005-01-01

    In terms of the carrier-to-interference-ratio, the performance of co-channel interference in cellular communications systems is studied. The approach is based on an improved analysis, which allows to take into account some area in the desired sector may not be interfered by some co-channel sectors with exact geometrical analysis, instead of the entire sector interfered by some co-channel sectors. Other features, such as power control and the number of interferences are also included.

  9. Investigation of Vehicles as Probes Using Global Positioning System and Cellular Phone Tracking: Field Operational Test

    OpenAIRE

    Yim, Y. B. Youngbin; Cayford, Randall

    2001-01-01

    This paper reports on the first phase of the location technology evaluation for probe vehicles. Two technologies were evaluated, Global Positioning Systems (GPS) and the cellular phone tracking technology developed by US Wireless. Although GPS has shown great potential for vehicle probes, much of the previous research is theoretical in nature. Very little work has been done in the areas of experimental research, implementation or deployment. Most of the field tests were anecdotal; a systemati...

  10. Stochastic Ordering based Carrier-to-Interference Ratio Analysis for the Shotgun Cellular Systems

    CERN Document Server

    Madhusudhanan, Prasanna; Youjian,; Liu,; Brown, Timothy X; Baker, Kenneth R

    2011-01-01

    A simple analytical tool based on stochastic ordering is developed to compare the distributions of carrier-to-interference ratio at the mobile station of two cellular systems where the base stations are distributed randomly according to certain non-homogeneous Poisson point processes. The comparison is conveniently done by studying only the base station densities without having to solve for the distributions of the carrier-to-interference ratio, that are often hard to obtain.

  11. Molecular biophysics: detection and characterization of damage in molecular, cellular, and physiological systems

    International Nuclear Information System (INIS)

    This section contains summaries of research on the detection and characterization of damage in molecular, cellular, and physiological systems. Projects under investigation in this section include: chemical synthesis of nucleic acid derivatives; structural and conformational properties of biological molecules in solution; crystallographic and chemical studies of immunoglobulin structure; instrument design and development for x-ray and neutron scattering studies of biological molecules; and chromobiology and circadian regulation

  12. An Exact Path-Loss Density Model for Mobiles in a Cellular System

    OpenAIRE

    Abdulla, Mouhamed; Shayan, Yousef R.

    2013-01-01

    In trying to emulate the spatial position of wireless nodes for purpose of analysis, we rely on stochastic simulation. And, it is customary, for mobile systems, to consider a base-station radiation coverage by an ideal cell shape. For cellular analysis, a hexagon contour is always preferred mainly because of its tessellating nature. Despite this fact, largely due to its intrinsic simplicity, in literature only random dispersion model for a circular shape is known. However, if considered, this...

  13. A Novel Mathematical Model Describing Adaptive Cellular Drug Metabolism and Toxicity in the Chemoimmune System

    OpenAIRE

    Tóth, Attila; Brózik, Anna; Szakács, Gergely; Sarkadi, Balázs; Hegedüs, Tamás

    2015-01-01

    Cells cope with the threat of xenobiotic stress by activating a complex molecular network that recognizes and eliminates chemically diverse toxic compounds. This “chemoimmune system” consists of cellular Phase I and Phase II metabolic enzymes, Phase 0 and Phase III ATP Binding Cassette (ABC) membrane transporters, and nuclear receptors regulating these components. In order to provide a systems biology characterization of the chemoimmune network, we designed a reaction kinetic model based on d...

  14. Site-specific recombinatorics: in situ cellular barcoding with the Cre Lox system

    OpenAIRE

    Weber, Tom S.; Dukes, Mark; Miles, Denise; Glaser, Stefan; Naik, Shalin; Duffy, Ken R.

    2016-01-01

    Cellular barcoding is a significant, recently developed, biotechnology tool that enables the familial identification of progeny of individual cells in vivo. Most existing approaches rely on ex vivo viral transduction of cells with barcodes, followed by adoptive transfer into an animal, which works well for some systems, but precludes barcoding cells in their native environment, such as those inside solid tissues. With a view to overcoming this limitation, we propose a new design for a genetic...

  15. Mobile Radio Communications: Second and Third Generation Cellular and WATM Systems: 2nd

    OpenAIRE

    Steele, R; Hanzo, L

    1999-01-01

    This comprehensive all-in-one reference work covers the fundamental physical aspects of mobile communications and explains the latest techniques employed in second and third generation digital cellular mobile radio systems. Mobile radio communications technology has progressed rapidly and it is now capable of the transmission of voice, data and image signals. This new edition reflects the current state-of-the-art by featuring: * Expanded and updated sections on voice compression techniques, i...

  16. Generalized Carrier to Interference Ratio Analysis for the Shotgun Cellular System

    CERN Document Server

    Madhusudhanan, Prasanna; Liu, Youjian; Brown, Timothy X; Baker, Kenneth

    2010-01-01

    In this paper, the performance of cellular systems in which the base-stations (BS) are randomly placed (so-called shotgun cellular system, SCS) is studied. Previous results for a uniform 2-dimensional distribution of BS are generalized to non-uniform placement of BS in any dimension. The carrier-to-interference ratio (CIR) and the carrier-to-interference-plus-noise ratio (CINR) are analyzed for dense and sparse BS density cellular systems, respectively. A semi-analytical expression for the tail probability of the CIR is derived. Additionally, a tool for comparing the CIR performances of two SCSs based on BS densities is presented. With these results, we completely characterize the performance of a SCS with an arbitrary BS density function. Next, a SCS affected by random shadow fading is shown to be equivalent to another SCS without shadow fading and a different BS density function, and hence the performance of a uniform l-D SCS is independent of shadow fading. Thus, for a sparse uniform l-D SCS, the effect of...

  17. Core Principles for Talent Management System and its Impact on Competitive Advantage "Applied Study Cellular Communications Companies in Jordan"

    OpenAIRE

    Mohammad Fathi Almaaitah; Yoshifumi Harada; Mohd Foad Bin Sakdan

    2015-01-01

    The aim of this study is to measure the core principles of talent management system and its impact on competitive advantage in Cellular communications companies in Jordan, The population of the study consisted of Three cellular telecom companies operating in the Jordanian market, the study sample was selected a random sample of employees in administrative levels: top management and middle management and supervisors in cellular communications companies operating in Jordan, and around include (...

  18. Putative Antiparasite Defensive System Involving Ribosomal and Nonribosomal Oligopeptides in Cyanobacteria of the Genus Planktothrix

    OpenAIRE

    Rohrlack, Thomas; Christiansen, Guntram; Kurmayer, Rainer

    2013-01-01

    Parasitic chytrid fungi can inflict significant mortality on cyanobacteria but frequently fail to keep cyanobacterial dominance and bloom formation in check. Our study tested whether oligopeptide production, a common feature in many cyanobacteria, can be a defensive mechanism against chytrid parasitism. The study employed the cyanobacterial strain Planktothrix NIVA-CYA126/8 and its mutants with knockout mutations for microcystins, anabaenopeptins, and microviridins, major oligopeptide classes...

  19. Lead tolerance mechanism in Conyza canadensis: subcellular distribution, ultrastructure, antioxidative defense system, and phytochelatins.

    Science.gov (United States)

    Li, Ying; Zhou, Chuifan; Huang, Meiying; Luo, Jiewen; Hou, Xiaolong; Wu, Pengfei; Ma, Xiangqing

    2016-03-01

    We used hydroponic experiments to examine the effects of different concentrations of lead (Pb) on the performance of the Pb-tolerable plant Conyza canadensis. In these experiments, most of the Pb was accumulated in the roots; there was very little Pb accumulated in stems and leaves. C. canadensis is able to take up significant amounts of Pb whilst greatly restricting its transportation to specific parts of the aboveground biomass. High Pb concentrations inhibited plant growth, increased membrane permeability, elevated antioxidant enzyme activity in roots, and caused a significant increase in root H2O2 and malondialdehyde content. Analysis of Pb content at the subcellular level showed that most Pb was associated with the cell wall fraction, followed by the nucleus-rich fraction, and with a minority present in the mitochondrial and soluble fractions. Furthermore, transmission electron microscopy and energy dispersive X-ray analysis of root cells revealed that the cell wall and intercellular space in C. canadensis roots are the main locations of Pb accumulation. Additionally, high Pb concentrations adversely affected the cellular structure of C. canadensis roots. The increased enzyme activity suggests that the antioxidant system may play an important role in eliminating or alleviating Pb toxicity in C. canadensis roots. However, the levels of non-protein sulfhydryl compounds, glutathione, and phytochelatin did not significantly change in either the roots or leaves under Pb-contaminated treatments. Our results provide strong evidence that cell walls restrict Pb uptake into the root and act as an important barrier protecting root cells, while demonstrating that antioxidant enzyme levels are correlated with Pb exposure. These findings demonstrate the roles played by these detoxification mechanisms in supporting Pb tolerance in C. canadensis. PMID:26733305

  20. Cognitive Cellular Systems: A New Challenge on the RF Analog Frontend

    Science.gov (United States)

    Varga, Gabor; Schrey, Moritz; Subbiah, Iyappan; Ashok, Arun; Heinen, Stefan

    2016-07-01

    Cognitive Cellular Systems are seen today as one of the most promising ways of moving forward solving or at least easing the still worsening situation of congested spectrum caused by the growing number of users and the expectation of higher data transfer rates. As the intelligence of a Cognitive Radio system is located in the digital domain - the Cognitive Engine and associated layers - extensive research has been ongoing in that domain since Mitola published his idea in 1999. Since, a big progress has been made in the domain of architectures and algorithms making systems more efficient and highly flexible. The pace of this progress, however, is going to be impeded by hard requirements on the received and transmitted signal quality, introducing ultimate challenges on the performance of the RF analog frontend, such as in-band local oscillator harmonics, ultra low sensitivity and ultra high linearity. The RF frontend is thus likely to become the limiting technical factor in the true realization of a Cognitive Cellular System. Based on short recapitulations of the most crucial issues in RF analog design for Cognitive Systems, this article will point out why those mechanisms become responsible for the limitation of the overall performance particularly in a broadband Cognitive Cellular System. Furthermore, as part of a possible solution to ease the situation, system design of a high intermediate frequency (IF) to UHF frequency converter for cognitive radios is discussed and the performance of such a converter analyzed as a proof of concept. In addition to successfully tackling some of the challenges, such a high-IF converter enables white space operation for existing commercial devices by acting as frequency converter. From detailed measurements, the capabilities in both physical layer and application layer performance of a high-IF frontend developed out of off-the-shelf components is explained and is shown to provide negligible degradation to the commercial device

  1. PHENOMENON OF PSYCHOLOGICAL DEFENSE

    OpenAIRE

    Elena T. Sokolova

    2011-01-01

    The author discusses the controversial issues of formation and functioning of psychological defense mechanisms in ontogenesis and in personality disorders as they are represented in classical and contemporary psychoanalysis, in cognitivism and communication theory. The paper emphasizes the role of cognitive organization (style), sign-symbolic mediation, representative system of object relations and attachments in individual typological variability of the level organization of defense mechanis...

  2. [Peculiarities of antioxidant defense system in erythroid cells and tissues of pigs under action of chromium chloride].

    Science.gov (United States)

    Iskra, R Ia; Vlizlo, V V

    2013-01-01

    The influence of CrCI3 in the amount of 400 mg Cr/kg of feed on antioxidant defense in populations of erythrocytes, erythroid bone marrow cells and tissues of pigs was studied. The increasing of the antioxidant defense of swine organism, as evidenced by the increase in superoxide dismutase and glutathione peroxidase activity in the fractions of "young" erythrocytes, was shown. Superoxide dismutase activity decreases, while glutathione and catalase activity increases in the erythroid cells of the bone marrow after of CrCl3 action. Oxidative processes are intensified in the liver of pigs of the experimental group, in contrast to other tissues, leading to the increase of content of TBARS-products, growth of superoxide dismutase activity and reduction of glutathione peroxidase activity. At the same time, the action of CrCl3 in other tissues activates antioxidant system, including the kidneys, lungs and myocardium, increases superoxide dismutase activity, and catalase activity in the spleen and kidneys. A decrease of content of TBARS-products and reduction of superoxide dismutase activity, as well as the increase of katalase activity and reduction of glutathione content were discovered in the skeletal muscles of pigs of the experimental group. As a result of research it is suggested to add CrCl3 to the diet of pigs to enhance antioxidant defense during their intensive growth. PMID:23940874

  3. ELR chemokine signaling in host defense and disease in a viral model of central nervous system disease

    Directory of Open Access Journals (Sweden)

    Martin P Hosking

    2014-06-01

    Full Text Available Intracranial infection of the neurotropic JHM strain of mouse hepatitis virus (JHMV into the central nervous system (CNS of susceptible strains of mice results in an acute encephalomyelitis, accompanied by viral replication in glial cells and robust infiltration of virus-specific T cells that contribute to host defense through cytokine secretion and cytolytic activity. Mice that survive the acute stage of disease develop an immune-mediated demyelinating diseases characterized by viral persistence in white matter tracts and a chronic neuroinflammatory response dominated by T cells and macrophages. Early following JHMV infection, there is a dynamic expression of chemokines and chemokine receptors that contribute to neuroinflammation by regulating innate and adaptive immune responses as well influencing glial biology. In response to JHMV infection, we have shown that signaling through the chemokine receptor CXCR2 contributes to host defense through recruitment of polymorphonuclear cells (PMNs to the CNS that enhance permeability of the blood-brain-barrier (BBB and facilitating entry of virus-specific T cells into the parenchyma. Further, CXCR2 promotes the protection of oligodendroglia from cytokine-induced apoptosis and restricts the severity of demyelination. This review covers aspects related to the role of CXCR2 in host defense and disease in response to JHMV infection.

  4. A Geometrical-Based Model for Cochannel Interference Analysis and Capacity Estimation of CDMA Cellular Systems

    Directory of Open Access Journals (Sweden)

    Konstantinos B. Baltzis

    2008-10-01

    Full Text Available A common assumption in cellular communications is the circular-cell approximation. In this paper, an alternative analysis based on the hexagonal shape of the cells is presented. A geometrical-based stochastic model is proposed to describe the angle of arrival of the interfering signals in the reverse link of a cellular system. Explicit closed form expressions are derived, and simulations performed exhibit the characteristics and validate the accuracy of the proposed model. Applications in the capacity estimation of WCDMA cellular networks are presented. Dependence of system capacity of the sectorization of the cells and the base station antenna radiation pattern is explored. Comparisons with data in literature validate the accuracy of the proposed model. The degree of error of the hexagonal and the circular-cell approaches has been investigated indicating the validity of the proposed model. Results have also shown that, in many cases, the two approaches give similar results when the radius of the circle equals to the hexagon inradius. A brief discussion on how the proposed technique may be applied to broadband access networks is finally made.

  5. Dynamic mapping of flood boundaries: current possibilities offered by Earth Observation System and Cellular Automata

    Directory of Open Access Journals (Sweden)

    A. Gerardi

    2014-01-01

    Full Text Available Flooding is an ongoing and complex problem in Italy. Very large floods caused inundation of the closest areas to the city centre in Rome in 1937, 1976, 1992, 2005 and most recently in 2008. Rome is located at the bottom of the Tiber River catchment, which cover an area of 16 000 km2. Intense precipitations struck the Tyrrhenian Sea side of the peninsula inducing a flood event on the Tiber and Aniene's (Tiber's tributary basins – which captured the attention of the national and international media. Actually there is no validated model in operation for real-time flood forecasting. This research aims at comparing the Cellular Model CAESAR (Cellular Automation Evolutionary Slope And River application on a reach of the Aniene River with Earth Observation Systems. The main result expected is the prediction of future channel dynamics on short and medium time scale.

  6. A Pedestrian Navigation System Using Cellular Phone Video-Conferencing Functions

    Directory of Open Access Journals (Sweden)

    Akihiko Sugiura

    2012-01-01

    Full Text Available A user’s position-specific field has been developed using the Global Positioning System (GPS technology. To determine the position using cellular phones, a device was developed, in which a pedestrian navigation unit carries the GPS. However, GPS cannot specify a position in a subterranean environment or indoors, which is beyond the reach of transmitted signals. In addition, the position-specification precision of GPS, that is, its resolution, is on the order of several meters, which is deemed insufficient for pedestrians. In this study, we proposed and evaluated a technique for locating a user’s 3D position by setting up a marker in the navigation space detected in the image of a cellular phone. By experiment, we verified the effectiveness and accuracy of the proposed method. Additionally, we improved the positional precision because we measured the position distance using numerous markers.

  7. BioXyce: an engineering platform for the study of cellular systems.

    Science.gov (United States)

    May, E E; Schiek, R L

    2009-03-01

    Researchers use constructs from the field of electrical engineering for the modelling and analysis of biological systems, but few exploit parallels between electrical and biological circuits for simulation purposes. The authors discuss the development of BioXyce, a circuit-based biological simulation platform that uses Xyce, a large-scale electrical circuit simulator, as its simulation engine. BioXyce is capable of simulating whole-cell and multicellular systems. Simulation results for the central metabolism in Escherichia coli K12 and cellular differentiation in Drosophila sp. are presented. PMID:19292562

  8. Estimation of the Carrier-to-Interference Ratio in Cellular Radio Systems

    OpenAIRE

    Gunnarsson, Fredrik; Blom, Jonas; Gustafsson, Fredrik

    1999-01-01

    The problem to track time-varying parameters in cellular radio systems is studied. The focus is on estimation based only on the signals that are readily available. Previous work has demonstrated very good performance relying on analog measurement. In a real system most of the information is lost due to quantization and sampling at a rate that might be as low as 2 Hz (GSM case). Therefore a different approach is required and for that matter a maximum likelihood estimator has been designed and ...

  9. An asynchronous communication system based on the hyperchaotic system of 6th-order cellular neural network

    Science.gov (United States)

    Wang, Xingyuan; Xu, Bing; Luo, Chao

    2012-11-01

    This paper proposes a novel asynchronous communication scheme. Based on this scheme, a model using the hyperchaotic system of 6th-order Cellular Neural Network (CNN) is designed. This scheme enhances the security of asynchronous communication compared to the conventional ones. It is noteworthy that the proposed communication scheme does not depend on synchronization, and almost all chaotic systems can be involved in this scheme. Numerical simulations show the effectiveness of this scheme.

  10. Defense on the Move: Ant-Based Cyber Defense

    Energy Technology Data Exchange (ETDEWEB)

    Fink, Glenn A.; Haack, Jereme N.; McKinnon, Archibald D.; Fulp, Errin W.

    2014-04-15

    Many common cyber defenses (like firewalls and IDS) are as static as trench warfare allowing the attacker freedom to probe them at will. The concept of Moving Target Defense (MTD) adds dynamism to the defender side, but puts the systems to be defended themselves in motion, potentially at great cost to the defender. An alternative approach is a mobile resilient defense that removes attackers’ ability to rely on prior experience without requiring motion in the protected infrastructure itself. The defensive technology absorbs most of the cost of motion, is resilient to attack, and is unpredictable to attackers. The Ant-Based Cyber Defense (ABCD) is a mobile resilient defense providing a set of roaming, bio-inspired, digital-ant agents working with stationary agents in a hierarchy headed by a human supervisor. The ABCD approach provides a resilient, extensible, and flexible defense that can scale to large, multi-enterprise infrastructures like the smart electric grid.

  11. The evolution of early cellular systems viewed through the lens of biological interactions

    Directory of Open Access Journals (Sweden)

    Anthony M Poole

    2015-10-01

    Full Text Available The minimal cell concept represents a pragmatic approach to the question of how few genes are required to run a cell. This is a helpful way to build a parts-list, and has been more successful than attempts to deduce a minimal gene set for life by inferring the gene repertoire of the Last Universal Common Ancestor, as few genes trace back to this hypothetical ancestral state. However, the study of minimal cellular systems is the study of biological outliers where, by practical necessity, coevolutionary interactions are minimised or ignored. In this paper, we consider the biological context from which minimal genomes have been removed. For instance, some of the most reduced genomes are from endosymbionts and are the result of coevolutionary interactions with a host; few such organisms are ‘free-living’. As few, if any, biological systems exist in complete isolation, we expect that, as with modern life, early biological systems were part of an ecosystem, replete with organismal interactions. We favour refocusing discussions of the evolution of cellular systems on processes rather than gene counts. We therefore draw a distinction between a pragmatic minimal cell (an interesting engineering problem, a distributed genome (a system resulting from an evolutionary transition involving more than one cell and the looser coevolutionary interactions that are ubiquitous in ecosystems. Finally, we consider the distributed genome and coevolutionary interactions between genomic entities in the context of early evolution.

  12. Cellular resilience.

    Science.gov (United States)

    Smirnova, Lena; Harris, Georgina; Leist, Marcel; Hartung, Thomas

    2015-01-01

    Cellular resilience describes the ability of a cell to cope with environmental changes such as toxicant exposure. If cellular metabolism does not collapse directly after the hit or end in programmed cell death, the ensuing stress responses promote a new homeostasis under stress. The processes of reverting "back to normal" and reversal of apoptosis ("anastasis") have been studied little at the cellular level. Cell types show astonishingly similar vulnerability to most toxicants, except for those that require a very specific target, metabolism or mechanism present only in specific cell types. The majority of chemicals triggers "general cytotoxicity" in any cell at similar concentrations. We hypothesize that cells differ less in their vulnerability to a given toxicant than in their resilience (coping with the "hit"). In many cases, cells do not return to the naive state after a toxic insult. The phenomena of "pre-conditioning", "tolerance" and "hormesis" describe this for low-dose exposures to toxicants that render the cell more resistant to subsequent hits. The defense and resilience programs include epigenetic changes that leave a "memory/scar" - an alteration as a consequence of the stress the cell has experienced. These memories might have long-term consequences, both positive (resistance) and negative, that contribute to chronic and delayed manifestations of hazard and, ultimately, disease. This article calls for more systematic analyses of how cells cope with toxic perturbations in the long-term after stressor withdrawal. A technical prerequisite for these are stable (organotypic) cultures and a characterization of stress response molecular networks. PMID:26536287

  13. Polyamines Confer Salt Tolerance in Mung Bean (Vigna radiata L.) by Reducing Sodium Uptake, Improving Nutrient Homeostasis, Antioxidant Defense, and Methylglyoxal Detoxification Systems

    Science.gov (United States)

    Nahar, Kamrun; Hasanuzzaman, Mirza; Rahman, Anisur; Alam, Md. Mahabub; Mahmud, Jubayer-Al; Suzuki, Toshisada; Fujita, Masayuki

    2016-01-01

    The physiological roles of PAs (putrescine, spermidine, and spermine) were investigated for their ability to confer salt tolerance (200 mM NaCl, 48 h) in mung bean seedlings (Vigna radiata L. cv. BARI Mung-2). Salt stress resulted in Na toxicity, decreased K, Ca, Mg, and Zn contents in roots and shoots, and disrupted antioxidant defense system which caused oxidative damage as indicated by increased lipid peroxidation, H2O2 content, O2•- generation rate, and lipoxygenase activity. Salinity-induced methylglyoxal (MG) toxicity was also clearly evident. Salinity decreased leaf chlorophyll (chl) and relative water content (RWC). Supplementation of salt affected seedlings with exogenous PAs enhanced the contents of glutathione and ascorbate, increased activities of antioxidant enzymes (dehydroascorbate reductase, glutathione reductase, catalase, and glutathione peroxidase) and glyoxalase enzyme (glyoxalase II), which reduced salt-induced oxidative stress and MG toxicity, respectively. Exogenous PAs reduced cellular Na content and maintained nutrient homeostasis and modulated endogenous PAs levels in salt affected mung bean seedlings. The overall salt tolerance was reflected through improved tissue water and chl content, and better seedling growth. PMID:27516763

  14. AN EFFICIENT RADIO RESOURCE MANAGEMENT STRATEGY FOR ADAPTIVE OFDM CELLULAR SYSTEMS

    Institute of Scientific and Technical Information of China (English)

    Yu Guanding; Zhang Zhaoyang; Qiu Peiliang

    2006-01-01

    This paper presents an efficient Radio Resource Management (RRM) strategy for adaptive Orthogonal Frequency Division Multiplexing (OFDM) cellular systems. In the proposed strategy, only those users who have the same distance from their base stations can reuse a same subcarrier. This can guarantee the received Carrier-to-Interference ratio (C/I) of each subcarrier to be acceptable as required by system planning. Then by employing different modulation scheme on each subcarrier according to its received C/I, system spectral efficiency can be gracefully increased. Analytical and simulation results show that the spectral efficiency is improved by 40% without sacrificing the Bit Error Rate (BER) performance and call blocking probability and system capacity of the proposed strategy is better than conventional systems.

  15. SELF-ADAPTIVE CONTROLS OF A COMPLEX CELLULAR SIGNALING TRANSDUCTION SYSTEM

    Institute of Scientific and Technical Information of China (English)

    LI Hong; ZHOU Zhiyuan; DAI Rongyang; LUO Bo; ZHENG Xiaoli; YANG Wenli; HE Tao; WU Minglu

    2004-01-01

    In cells, the interactions of distinct signaling transduction pathways originating from cross-talkings between signaling molecules give rise to the formation of signaling transduction networks, which contributes to the changes (emergency) of kinetic behaviors of signaling system compared with single molecule or pathway. Depending on the known experimental data, we have constructed a model for complex cellular signaling transduction system, which is derived from signaling transduction of epidermal growth factor receptor in neuron. By the computational simulating methods, the self-adaptive controls of this system have been investigated. We find that this model exhibits a relatively stable selfadaptive system, especially to over-stimulation of agonist, and the amplitude and duration of signaling intermediates in it could be controlled by multiple self-adaptive effects, such as "signal scattering", "positive feedback", "negative feedback" and "B-Raf shunt". Our results provide an approach to understanding the dynamic behaviors of complex biological systems.

  16. DNA Mismatch Repair System: Repercussions in Cellular Homeostasis and Relationship with Aging

    Directory of Open Access Journals (Sweden)

    Juan Cristóbal Conde-Pérezprina

    2012-01-01

    Full Text Available The mechanisms that concern DNA repair have been studied in the last years due to their consequences in cellular homeostasis. The diverse and damaging stimuli that affect DNA integrity, such as changes in the genetic sequence and modifications in gene expression, can disrupt the steady state of the cell and have serious repercussions to pathways that regulate apoptosis, senescence, and cancer. These altered pathways not only modify cellular and organism longevity, but quality of life (“health-span”. The DNA mismatch repair system (MMR is highly conserved between species; its role is paramount in the preservation of DNA integrity, placing it as a necessary focal point in the study of pathways that prolong lifespan, aging, and disease. Here, we review different insights concerning the malfunction or absence of the DNA-MMR and its impact on cellular homeostasis. In particular, we will focus on DNA-MMR mechanisms regulated by known repair proteins MSH2, MSH6, PMS2, and MHL1, among others.

  17. Synchronization, TIGoRS, and Information Flow in Complex Systems: Dispositional Cellular Automata.

    Science.gov (United States)

    Sulis, William H

    2016-04-01

    Synchronization has a long history in physics where it refers to the phase matching of two identical oscillators. This notion has been extensively studied in physics as well as in biology, where it has been applied to such widely varying phenomena as the flashing of fireflies and firing of neurons in the brain. Human behavior, however, may be recurrent but it is not oscillatory even though many physiological systems do exhibit oscillatory tendencies. Moreover, much of human behaviour is collaborative and cooperative, where the individual behaviours may be distinct yet contemporaneous (if not simultaneous) and taken collectively express some functionality. In the context of behaviour, the important aspect is the repeated co-occurrence in time of behaviours that facilitate the propagation of information or of functionality, regardless of whether or not these behaviours are similar or identical. An example of this weaker notion of synchronization is transient induced global response synchronization (TIGoRS). Previous work has shown that TIGoRS is a ubiquitous phenomenon among complex systems, enabling them to stably parse environmental transients into salient units to which they stably respond. This leads to the notion of Sulis machines, which emergently generate a primitive linguistic structure through their dynamics. This article reviews the notion of TIGoRS and its expression in several complex systems models including tempered neural networks, driven cellular automata and cocktail party automata. The emergent linguistics of Sulis machines are discussed. A new class of complex systems model, the dispositional cellular automaton is introduced. A new metric for TIGoRS, the excess synchronization, is introduced and applied to the study of TIGoRS in dispositional cellular automata. It is shown that these automata exhibit a nonlinear synchronization response to certain perturbing transients. PMID:27033136

  18. Erosion/corrosion concerns in feed preparation systems at the Defense Waste Processing Facility

    International Nuclear Information System (INIS)

    The Savannah River Site (SRS) has been operating a nuclear fuel cycle since the 1950's to produce nuclear materials in support of the national defense effort. The Department of Energy authorized the construction of the Defense Waste Processing Facility (DWPF) to immobilize the high level radioactive waste resulting from these processes as a durable borosilicate glass. The DWPF, after having undergone extensive testing, has been approved for operations and is currently immobilizing radioactive waste. To ensure reliability of the DWPF remote canyon processing equipment, a materials evaluation program was performed prior to radioactive operations to determine to what extent erosion/corrosion would impact design life of equipment. The program consisted of performing pre-service baseline inspections on critical equipment and follow-up inspections after completion of DWPF cold chemical demonstration runs. Non-destructive examination (NDE) techniques were used to assess erosion/corrosion as well as evaluation of corrosion coupon racks. These results were used to arrive at predicted equipment life for selected feed preparation equipment. It was concluded with the exception of the coil and agitator for the slurry mix evaporator (SME), which are exposed to erosive glass frit particles, all of the equipment should meet its design life

  19. Disruption of non-enzymatic antioxidant defense systems in the brain of rats with water-immersion restraint stress

    OpenAIRE

    Ohta, Yoshiji; Yashiro, Koji; Ohashi, Koji; Imai, Yoichiro

    2012-01-01

    We examined whether non-enzymatic antioxidant defense systems are disrupted in the brain of rats with water-immersion restraint stress. When rats were exposed to water-immersion restraint stress for 1.5, 3 or 6 h, the brain had decreased ascorbic acid and reduced glutathione contents and increased lipid peroxide and nitric oxide metabolites contents at 3 h and showed further changes in these components with a reduction of vitamin E content at 6 h. Increased serum levels of stress markers were...

  20. Characterization of inhibitors of phosphodiesterase 1C on a human cellular system.

    Science.gov (United States)

    Dunkern, Torsten R; Hatzelmann, Armin

    2007-09-01

    Different inhibitors of the Ca(2+)/calmodulin-stimulated phosphodiesterase 1 family have been described and used for the examination of phosphodiesterase 1 in cellular, organ or animal models. However, the inhibitors described differ in potency and selectivity for the different phosphodiesterase family enzymes, and in part exhibit additional pharmacodynamic actions. In this study, we demonstrate that phosphodiesterase 1C is expressed in the human glioblastoma cell line A172 with regard to mRNA, protein and activity level, and that lower activities of phosphodiesterase 2, phosphodiesterase 3, phosphodiesterase 4 and phosphodiesterase 5 are also present. The identity of the phosphodiesterase 1C activity detected was verified by downregulation of the mRNA and protein through human phosphodiesterase 1C specific small interfering RNA. In addition, the measured K(m) values (cAMP, 1.7 microm; cGMP, 1.3 microm) are characteristic of phosphodiesterase 1C. We demonstrate that treatment with the Ca(2+) ionophore ionomycin increases intracellular Ca(2+) in a concentration-dependent way without affecting cell viability. Under conditions of enhanced intracellular Ca(2+) concentration, a rapid increase in cAMP levels caused by the adenylyl cyclase activator forskolin was abolished, indicating the involvement of Ca(2+)-activated phosphodiesterase 1C. The reduction of forskolin-stimulated cAMP levels was reversed by phosphodiesterase 1 inhibitors in a concentration-dependent way. Using this cellular system, we compared the cellular potency of published phosphodiesterase 1 inhibitors, including 8-methoxymethyl-3-isobutyl-1-methylxanthine, vinpocetine, SCH51866, and two established phosphodiesterase 1 inhibitors developed by Schering-Plough (named compounds 31 and 30). We demonstrate that up to 10 microm 8-methoxymethyl-3-isobutyl-1-methylxanthine and vinpocetine had no effect on the reduction of forskolin-stimulated cAMP levels by ionomycin, whereas the more selective and up to 10

  1. Phase-Type Models of Channel-Holding Times in Cellular Communication Systems

    DEFF Research Database (Denmark)

    Christensen, Thomas Kaare; Nielsen, Bo Friis; Iversen, Villy Bæk

    2004-01-01

    In this paper, we derive the distribution of the channel-holding time when both cell-residence and call-holding times are phase-type distributed. Furthermore, the distribution of the number of handovers, the conditional channel-holding time distributions, and the channel-holding time when cell re...... residence times are correlated are derived. All distributions are of phase type, making them very general and flexible. The channel-holding times are of importance in performance evaluation and simulation of cellular mobile communication systems....

  2. In Silico Modeling of the Immune System: Cellular and Molecular Scale Approaches

    Directory of Open Access Journals (Sweden)

    Mariagrazia Belfiore

    2014-01-01

    Full Text Available The revolutions in biotechnology and information technology have produced clinical data, which complement biological data. These data enable detailed descriptions of various healthy and diseased states and responses to therapies. For the investigation of the physiology and pathology of the immune responses, computer and mathematical models have been used in the last decades, enabling the representation of biological processes. In this modeling effort, a major issue is represented by the communication between models that work at cellular and molecular level, that is, multiscale representation. Here we sketch some attempts to model immune system dynamics at both levels.

  3. Evolution of the cellular communication system: An analysis in the Computational Paradigm

    International Nuclear Information System (INIS)

    We discuss the problem of the evolution of the cellular communication system from the RNA world to progenote to the modern cell. Our method analyses syntactical structure of molecular fossils in the non-coding regions of DNA within the information-processing gene model developed earlier. We concluded that sequence-specific binding is an ancient communication process with its origin in the RNA world. Moreover, we illustrate our viewpoint using four evolution snapshots from the first RNA segments, some 4.1. billion years ago, to the first cell, 3.8 billion years ago. (author). 31 refs

  4. Plant Defense against Insect Herbivores

    Directory of Open Access Journals (Sweden)

    Søren Bak

    2013-05-01

    Full Text Available Plants have been interacting with insects for several hundred million years, leading to complex defense approaches against various insect feeding strategies. Some defenses are constitutive while others are induced, although the insecticidal defense compound or protein classes are often similar. Insect herbivory induce several internal signals from the wounded tissues, including calcium ion fluxes, phosphorylation cascades and systemic- and jasmonate signaling. These are perceived in undamaged tissues, which thereafter reinforce their defense by producing different, mostly low molecular weight, defense compounds. These bioactive specialized plant defense compounds may repel or intoxicate insects, while defense proteins often interfere with their digestion. Volatiles are released upon herbivory to repel herbivores, attract predators or for communication between leaves or plants, and to induce defense responses. Plants also apply morphological features like waxes, trichomes and latices to make the feeding more difficult for the insects. Extrafloral nectar, food bodies and nesting or refuge sites are produced to accommodate and feed the predators of the herbivores. Meanwhile, herbivorous insects have adapted to resist plant defenses, and in some cases even sequester the compounds and reuse them in their own defense. Both plant defense and insect adaptation involve metabolic costs, so most plant-insect interactions reach a stand-off, where both host and herbivore survive although their development is suboptimal.

  5. Multi-objective group scheduling with learning effect in the cellular manufacturing system

    Directory of Open Access Journals (Sweden)

    Mohammad Taghi Taghavi-fard

    2011-01-01

    Full Text Available Group scheduling problem in cellular manufacturing systems consists of two major steps. Sequence of parts in each part-family and the sequence of part-family to enter the cell to be processed. This paper presents a new method for group scheduling problems in flow shop systems where it minimizes makespan (Cmax and total tardiness. In this paper, a position-based learning model in cellular manufacturing system is utilized where processing time for each part-family depends on the entrance sequence of that part. The problem of group scheduling is modeled by minimizing two objectives of position-based learning effect as well as the assumption of setup time depending on the sequence of parts-family. Since the proposed problem is NP-hard, two meta heuristic algorithms are presented based on genetic algorithm, namely: Non-dominated sorting genetic algorithm (NSGA-II and non-dominated rank genetic algorithm (NRGA. The algorithms are tested using randomly generated problems. The results include a set of Pareto solutions and three different evaluation criteria are used to compare the results. The results indicate that the proposed algorithms are quite efficient to solve the problem in a short computational time.

  6. Efficient Analysis of Systems Biology Markup Language Models of Cellular Populations Using Arrays.

    Science.gov (United States)

    Watanabe, Leandro; Myers, Chris J

    2016-08-19

    The Systems Biology Markup Language (SBML) has been widely used for modeling biological systems. Although SBML has been successful in representing a wide variety of biochemical models, the core standard lacks the structure for representing large complex regular systems in a standard way, such as whole-cell and cellular population models. These models require a large number of variables to represent certain aspects of these types of models, such as the chromosome in the whole-cell model and the many identical cell models in a cellular population. While SBML core is not designed to handle these types of models efficiently, the proposed SBML arrays package can represent such regular structures more easily. However, in order to take full advantage of the package, analysis needs to be aware of the arrays structure. When expanding the array constructs within a model, some of the advantages of using arrays are lost. This paper describes a more efficient way to simulate arrayed models. To illustrate the proposed method, this paper uses a population of repressilator and genetic toggle switch circuits as examples. Results show that there are memory benefits using this approach with a modest cost in runtime. PMID:26912276

  7. Translocation Biosensors – Cellular System Integrators to Dissect CRM1-Dependent Nuclear Export by Chemicogenomics

    Directory of Open Access Journals (Sweden)

    Roland H. Stauber

    2009-07-01

    Full Text Available Fluorescent protein biosensors are powerful cellular systems biology tools for dissecting the complexity of cellular processes with high spatial and temporal resolution. As regulated nucleo-cytoplasmic transport is crucial for the modulation of numerous (pathophysiological cellular responses, a detailed understanding of its molecular mechanism would open up novel options for a rational manipulation of the cell. In contrast to genetic approaches, we here established and employed high-content cellular translocation biosensors applicable for dissecting nuclear export by chemicogenomics. A431 cell lines, stably expressing a translocation biosensor composed of glutathione S-transferase, GFP and a rational combination of nuclear import and export signals, were engineered by antibiotic selection and flow cytometry sorting. Using an optimized nuclear translocation algorithm, the translocation response could be robustly quantified on the Cellomics Arrayscan® VTI platform. Subsequent to assay optimization, the assay was developed into a higher density 384-well format high-content assay and employed for the screening of the 17K ChemBioNet compound collection. This library was selected on the basis of a genetic algorithm used to identify maximum common chemical substructures in a database of annotated bioactive molecules and hence, is well-placed in the chemical space covered by bioactive compounds. Automated multiparameter data analysis combined with visual inspection allowed us to identify and to rationally discriminate true export inhibitors from false positives, which included fluorescent compounds or cytotoxic substances that dramatically affected the cellular morphology. A total of 120 potential hit compounds were selected for Cellomics Arrayscan® VTI based rescreening. The export inhibitory activity of 20 compounds effective at concentrations < 25 μM were confirmed by fluorescence microscopy in several cell lines. Interestingly, kinetic analysis

  8. Detection and Cellular Localization of Phospho-STAT2 in the Central Nervous System by Immunohistochemical Staining

    DEFF Research Database (Denmark)

    Khorooshi, Reza; Owens, Trevor

    2013-01-01

    Phosphorylation of signal transducers and activators of transcription (STATs) indicates their involvement in active signaling. Here we describe immunohistochemical staining procedures for detection and identification of the cellular localization of phospho-STAT2 in the central nervous system (CNS...

  9. DNA-controlled dynamic colloidal nanoparticle systems for mediating cellular interaction

    Science.gov (United States)

    Ohta, Seiichi; Glancy, Dylan; Chan, Warren C. W.

    2016-02-01

    Precise control of biosystems requires development of materials that can dynamically change physicochemical properties. Inspired by the ability of proteins to alter their conformation to mediate function, we explored the use of DNA as molecular keys to assemble and transform colloidal nanoparticle systems. The systems consist of a core nanoparticle surrounded by small satellites, the conformation of which can be transformed in response to DNA via a toe-hold displacement mechanism. The conformational changes can alter the optical properties and biological interactions of the assembled nanosystem. Photoluminescent signal is altered by changes in fluorophore-modified particle distance, whereas cellular targeting efficiency is increased 2.5 times by changing the surface display of targeting ligands. These concepts provide strategies for engineering dynamic nanotechnology systems for navigating complex biological environments.

  10. A federation of simulations based on cellular automata in cyber-physical systems

    Directory of Open Access Journals (Sweden)

    Hoang Van Tran

    2016-02-01

    Full Text Available In cyber-physical system (CPS, cooperation between a variety of computational and physical elements usually poses difficulties to current modelling and simulation tools. Although much research has proposed to address those challenges, most solutions do not completely cover uncertain interactions in CPS. In this paper, we present a new approach to federate simulations for CPS. A federation is a combination of, and coordination between simulations upon a standard of communication. In addition, a mixed simulation is defined as several parallel simulations federated in a common time progress. Such simulations run on the models of physical systems, which are built based on cellular automata theory. The experimental results are performed on a federation of three simulations of forest fire spread, river pollution diffusion and wireless sensor network. The obtained results can be utilized to observe and predict the behaviours of physical systems in their interactions.

  11. Reform of the planning, programming, budgeting system, and management control in the U.S. Department of Defense: insights fom budget theory

    OpenAIRE

    Jones, L R; McCaffery, Jerry L.

    2005-01-01

    Resource decision making for the Department of Defense (DOD) is one of the most challenging tasks in all of public financial management. DOD coordinates national security threat assessment, long- and intermediate-range planning and programming with annual budget formulation and execution. Between 2001 and 2004, Defense Secretary Donald Rumsfeld changed the system significantly, simplifying, and synchronizing it with presidential terms of office. We assess Planning, Programming, Budgeting S...

  12. Calcium Supplementation Improves Na+/K+ Ratio, Antioxidant Defense and Glyoxalase Systems in Salt-Stressed Rice Seedlings

    Science.gov (United States)

    Rahman, Anisur; Nahar, Kamrun; Hasanuzzaman, Mirza; Fujita, Masayuki

    2016-01-01

    The present study investigates the regulatory role of exogenous calcium (Ca) in developing salt stress tolerance in rice seedlings. Hydroponically grown 13-day-old rice (Oryza sativa L. cv. BRRI dhan47) seedlings were exposed to 200 mM NaCl alone and combined with 2 mM CaCl2 and 2 mM ethylene glycol tetraacetic acid (EGTA, a Ca scavenger) for 3 days. The salt stress caused growth inhibition, chlorosis and water shortage in the rice seedlings. The salt-induced stress disrupted ion homeostasis through Na+ influx and K+ efflux, and decreased other mineral nutrient uptake. Salt stress caused oxidative stress in seedlings through lipid peroxidation, loss of plasma membrane integrity, higher reactive oxygen species (ROS) production and methylglyoxal (MG) formation. The salt-stressed seedlings supplemented with exogenous Ca recovered from water loss, chlorosis and growth inhibition. Calcium supplementation in the salt-stressed rice seedlings improved ion homeostasis by inhibition of Na+ influx and K+ leakage. Exogenous Ca also improved ROS and MG detoxification by improving the antioxidant defense and glyoxalase systems, respectively. On the other hand, applying EGTA along with salt and Ca again negatively affected the seedlings as EGTA negated Ca activity. It confirms that, the positive responses in salt-stressed rice seedlings to exogenous Ca were for Ca mediated improvement of ion homeostasis, antioxidant defense and glyoxalase system.

  13. Oral Rg1 supplementation strengthens antioxidant defense system against exercise-induced oxidative stress in rat skeletal muscles

    Directory of Open Access Journals (Sweden)

    Yu Szu-Hsien

    2012-05-01

    Full Text Available Abstract Background Previous studies reported divergent results on nutraceutical actions and free radical scavenging capability of ginseng extracts. Variations in ginsenoside profile of ginseng due to different soil and cultivating season may contribute to the inconsistency. To circumvent this drawback, we assessed the effect of major ginsenoside-Rg1 (Rg1 on skeletal muscle antioxidant defense system against exhaustive exercise-induced oxidative stress. Methods Forty weight-matched rats were evenly divided into control (N = 20 and Rg1 (N = 20 groups. Rg1 was orally administered at the dose of 0.1 mg/kg bodyweight per day for 10-week. After this long-term Rg1 administration, ten rats from each group performed an exhaustive swimming, and remaining rats considered as non-exercise control. Tibialis anterior (TA muscles were surgically collected immediately after exercise along with non-exercise rats. Results Exhaustive exercise significantly (p Conclusions This study provide compelling evidences that Rg1 supplementation can strengthen antioxidant defense system in skeletal muscle and completely attenuate the membrane lipid peroxidation induced by exhaustive exercise. Our findings suggest that Rg1 can use as a nutraceutical supplement to buffer the exhaustive exercise-induced oxidative stress.

  14. Host-pathogen interactions between the human innate immune system and Candida albicans - Understanding and modeling defense and evasion strategies

    Directory of Open Access Journals (Sweden)

    Sybille eDühring

    2015-06-01

    Full Text Available The diploid, polymorphic yeast Candida albicans is one of the most important humanpathogenic fungi. C. albicans can grow, proliferate and coexist as a commensal on or within thehuman host for a long time. Alterations in the host environment, however, can render C. albicansvirulent. In this review, we describe the immunological cross-talk between C. albicans and thehuman innate immune system. We give an overview in form of pairs of human defense strategiesincluding immunological mechanisms as well as general stressors such as nutrient limitation,pH, fever etc. and the corresponding fungal response and evasion mechanisms. FurthermoreComputational Systems Biology approaches to model and investigate these complex interactionare highlighted with a special focus on game-theoretical methods and agent-based models. Anoutlook on interesting questions to be tackled by Systems Biology regarding entangled defenseand evasion mechanisms is given.

  15. Application of the defense-in-depth concept to qualify computer-based instrumentation and control systems important to safety

    International Nuclear Information System (INIS)

    In parallel to the technological development, the authorities and expert organisations are preparing the application of computer-based I and C to NPPs from the regulatory point of view. Generally the associated world-wide procedure follows steps like identification of safety issues, completion of the regulatory framework particularly regarding the licensing requirements and furthermore, recommendation of an appropriate set of qualification methods to prove that the requirements are met. The paper's intention is to show from the regulatory point of view that the choice as well as the combination of the qualification methods depend on system design features and development strategy. Similar as for the safety system design required, a defense-in-depth qualification concept is suggested to be helpful in order to prove that the computer-based system meets the licensing requirements. (author)

  16. Naringin abrogated radiation induced oxidative stress through modulation of redox regulated cellular signaling system

    International Nuclear Information System (INIS)

    Ionizing radiation is widely used as major diagnostic and therapeutic applications. However, the deleterious effects of ionizing radiation are due to generation of reactive oxygen species. The amounts of ionizing radiation that can be given to treat malignant tumours are often limited by toxicity in the surrounding normal tissues and organs. The aim of this study was to investigate the role of Naringin (NG), a natural flavonoid, present in many plant parts against radiation induced oxidative stress with an evidence based exploration of the mechanism involved. Isolated murine splenocyte were irradiated with γ radiation (6 Gy) along with/without different concentrations of NG (50 and 100 μM). Biochemical, immunoblot, flow cytometry and immunofluorescence study was subject to be performed to observe its molecular mechanisms of action. Pretreatment with NG significantly prevented the radiation induced intracellular ROS generation, therefore prevented cellular TBARS formation and development of cellular nitrite. NG showed the significant reduction in nuclear DNA damage with respect to irradiated splenocyte through inhibition of DNA-PKcs and p-γH2AX. It recovered radiation induced reduced cell viability through modulation of redox regulated cell signaling system. It resulted in significant inhibition of radiation induced G1/S phase cell cycle arrest mediated by modulation of p53 dependent p21/WAF1 expression followed by Cyclin E and CDK2 expression. NG was involved in blocking radiation induced p38 function; reversed radiation mediated differential stress response through inhibition of NF-κB pathway. It prevented p-IKKα/β, p-IκBα, p-p65, COX2 expression. It also reversed the radiation induced p38/NF-κB guided inflammatory development. Thus it down regulated radiation induced CRP, MCP-1, and iNOS2 gene expression. This novel role of naringin provides a basis for therapeutic applications in future against radiation induced molecular and cellular functional

  17. Riboflavin-induced Priming for Pathogen Defense in Arabidopsis thaliana

    Institute of Scientific and Technical Information of China (English)

    Shujian Zhang; Xue Yang; Maowu Sun; Feng Sun; Sheng Deng; Hansong Dong

    2009-01-01

    Riboflavin (vitamin B2) participates in a variety of redox processes that affect plant defense responses. Previously we have shown that riboflavin induces pathogen resistance in the absence of hypersensitive cell death (HCD) in plants. Herein, we report that riboflavin induces priming of defense responses in Arabidopsis thaliana toward infection by virulent Pseudomonas syringae pv. Tomato DC3000 (Pst). Induced resistance was mechanistically connected with the expression of defense response genes and cellular defense events, including H2O2 burst, HCD, and callose deposition in the plant. Riboflavin treatment and inoculation of plants with Pst were neither active but both synergized to induce defense responses. The priming process needed NPR1 (essential regulator of systemic acquired resistance) and maintenance of H2O2 burst but was independent of salicylic acid, jasmonic acid, ethylene, and abscisic acid. Our results suggest that the role of riboflavin in priming defenses is subject to a signaling process distinct from the known pathways of hormone signal transduction.

  18. Plant Defense against Insect Herbivores

    OpenAIRE

    Søren Bak; Joel Fürstenberg-Hägg; Mika Zagrobelny

    2013-01-01

    Plants have been interacting with insects for several hundred million years, leading to complex defense approaches against various insect feeding strategies. Some defenses are constitutive while others are induced, although the insecticidal defense compound or protein classes are often similar. Insect herbivory induce several internal signals from the wounded tissues, including calcium ion fluxes, phosphorylation cascades and systemic- and jasmonate signaling. These are perceived in undamaged...

  19. Phase transition in the economically modeled growth of a cellular nervous system

    CERN Document Server

    Nicosia, Vincenzo; Schafer, William R; Latora, Vito; Bullmore, Edward T; 10.1073/pnas.1300753110

    2013-01-01

    Spatially-embedded complex networks, such as nervous systems, the Internet and transportation networks, generally have non-trivial topological patterns of connections combined with nearly minimal wiring costs. However the growth rules shaping these economical trade-offs between cost and topology are not well understood. Here we study the cellular nervous system of the nematode worm C. elegans, together with information on the birth times of neurons and on their spatial locations. We find that the growth of this network undergoes a transition from an accelerated to a constant increase in the number of links (synaptic connections) as a function of the number of nodes (neurons). The time of this phase transition coincides closely with the observed moment of hatching, when development switches metamorphically from oval to larval stages. We use graph analysis and generative modelling to show that the transition between different growth regimes, as well as its coincidence with the moment of hatching, can be explain...

  20. Flux-driven cellular precipitation in open system to form porous Cu3Sn

    Science.gov (United States)

    Gusak, Andriy M.; Chen, Chih; Tu, K. N.

    2016-05-01

    Recently, a new morphology of porous Cu3Sn with lamellar structure is observed. Several possible explanations of the formation are proposed and compared. The most reasonable one seems to be the one based on a theory of flux-driven cellular precipitation in open system. Outflux of Sn from Cu6Sn5 generates simultaneously supersaturation with vacancies and with copper leading to the eutectoid-like transformation β → α + γ (where γ is void). The transformation is complete due to a complete outflux of Sn from the Cu6Sn5 phase. Simple formulae for prediction of the lamellar structure parameters and the propagation velocity are obtained and compared reasonably with experimental data. The suggested model can be interpreted as one more case of the flux-driven phase transformations in open systems.

  1. Role of Multi Agent System for Qos Guarantee in Cellular Networks

    Directory of Open Access Journals (Sweden)

    Nupur Giri

    2012-10-01

    Full Text Available The paper highlights the benefits of Multi Agent System for maintaining QoS in cellular network by evaluating various multi agent based call admission control strategies for different traffic conditions. It establishes the effect of degree of distribution of agents on system performance by comparing the two service architectures, namely centralized and distributed, for reactivity, responsiveness, utilization of resources, communication overhead, sustainability, scalability, robustness and modifiability. It also establishes the relationship between the social attitude of an agent towards the other agents and fairness of resource distribution in distributed architecture. This evaluation helps in building knowledge for choosing the optimal multi agent based call admission and channel borrowing schemes, along with the most suitable service architecture for the required QoS and traffic conditions.

  2. Cross-Layer Design and Analysis of Downlink Communications in Cellular CDMA Systems

    Directory of Open Access Journals (Sweden)

    Sun Jin Yuan

    2006-01-01

    Full Text Available A cellular CDMA network with voice and data communications is considered. Focusing on the downlink direction, we seek for the overall performance improvement which can be achieved by cross-layer analysis and design, taking physical layer, link layer, network layer, and transport layer into account. We are concerned with the role of each single layer as well as the interaction among layers, and propose algorithms/schemes accordingly to improve the system performance. These proposals include adaptive scheduling for link layer, priority-based handoff strategy for network admission control, and an algorithm for the avoidance of TCP spurious timeouts at the transport layer. Numerical results show the performance gain of each proposed scheme over independent performance of an individual layer in the wireless mobile network. We conclude that the system performance in terms of capacity, throughput, dropping probability, outage, power efficiency, delay, and fairness can be enhanced by jointly considering the interactions across layers.

  3. A mathematical model in cellular manufacturing system considering subcontracting approach under constraints

    Directory of Open Access Journals (Sweden)

    Kamran Forghani

    2012-10-01

    Full Text Available In this paper, a new mathematical model in cellular manufacturing systems (CMSs has been presented. In order to increase the performance of manufacturing system, the production quantity of parts has been considered as a decision variable, i.e. each part can be produced and outsourced, simultaneously. This extension would be minimized the unused capacity of machines. The exceptional elements (EEs are taken into account and would be totally outsourced to the external supplier in order to remove intercellular material handling cost. The problem has been formulated as a mixed-integer programming to minimize the sum of manufacturing variable costs under budget, machines capacity and demand constraints. Also, to evaluate advantages of the model, several illustrative numerical examples have been provided to compare the performance of the proposed model with the available classical approaches in the literature.

  4. Capturing asteroids into bound orbits around the earth: Massive early return on an asteroid terminal defense system

    International Nuclear Information System (INIS)

    Nuclear explosives may be used to capture small asteroids (e.g., 20--50 meters in diameter) into bound orbits around the earth. The captured objects could be used for construction material for manned and unmanned activity in Earth orbit. Asteroids with small approach velocities, which are the ones most likely to have close approaches to the Earth, require the least energy for capture. They are particularly easy to capture if they pass within one Earth radius of the surface of the Earth. They could be intercepted with intercontinental missiles if the latter were retrofit with a more flexible guiding and homing capability. This asteroid capture-defense system could be implemented in a few years at low cost by using decommissioned ICMs. The economic value of even one captured asteroid is many times the initial investment. The asteroid capture system would be an essential part of the learning curve for dealing with larger asteroids that can hit the earth

  5. Microbial community induces a plant defense system under growing on the lunar regolith analogue

    Science.gov (United States)

    Zaetz, Irina; Mytrokhyn, Olexander; Lukashov, Dmitry; Mashkovska, Svitlana; Kozyrovska, Natalia; Foing, Bernard H.

    The lunar rock considered as a potential source of chemical elements essential for plant nutrition, however, this substrate is of a low bioavailability. The use of microorganisms for decomposition of silicate rocks and stimulation of plant growth is a key idea in precursory scenario of growing pioneer plants for a lunar base (Kozyrovska et al., 2004; 2006; Zaetz et al., 2006). In model experiments a consortium of well-defined plant-associated bacteria were used for growing of French marigold (Tagetes patula L.) in anorthosite, analogous to a lunar rock. Inoculated plants appeared better seed germination, more fast development and also increased accumulation of K, Mg, Mn, Co, Cu and lowered level of the toxic Zn, Ni, Cr, comparing to control tagetes'. Bacteria regulate metal homeostasis in plants by changing their bioavailability and by stimulating of plant defense mechanisms. Inoculated plants were being accommodated to growth under stress conditions on anorthosite used as a substrate. In contrast, control plants manifested a heavy metal-induced oxidative stress, as quantified by protein carbonyl accumulation. Depending on the plant organ sampled and developmental stage there were increases or loses in the antioxidant enzyme activities (guaiacol peroxidase and glutathione-S-transferase). These changes were most evident in inoculated plants. Production of phenolic compounds, known as antioxidants and heavy metal chelators, is rised in variants of inoculated marigolds. Guaiacol peroxidase plays the main role, finally, in a reducing toxicity of heavy metals in plant leaves, while glutathione-S-transferase and phenolics overcome stress in roots.

  6. 77 FR 11355 - Defense Federal Acquisition Regulation Supplement; Business Systems-Definition and Administration...

    Science.gov (United States)

    2012-02-24

    ... systems, purchasing systems, earned value management systems (EVMS), material management and accounting... 252.215-7002, to clarify that the contractor's accounting system shall provide for management reviews...) assessing and approving/disapproving the six business systems, (2) making the decision to withhold...

  7. Cyber security level assignment for research reactor digital instrumentation and control system architecture using concept of defense in depth

    International Nuclear Information System (INIS)

    Due to recent aging of the analog instrumentation of many nuclear power plants (NPPs) and research reactors, the system reliability decreases while maintenance and testing costs increase. In addition, it is difficult to find the substitutable analog equipment s due to obsolescence. Therefore, the instrumentation and control (I and C) systems have changed from analog system to digital system due to these facts. With the introduction of digital systems, research reactors are forced to care for the problem of cyber attacks because I and C systems have been digitalized using networks or communication systems. Especially, it is more issued at research reactors due to the accessibility of human resources. In the real world, an IBM researcher has been successful in controlling the software by penetrating a NPPs network in U.S. on July 2008 and acquiring the control right of nuclear facilities after one week. Moreover, the malignant code called 'stuxnet' impaired the nearly 1,000 centrifugal separators in Iran according to an IAEA report. The problem of cyber attacks highlights the important of cyber security, which should be emphasized. Defense.in.depth (DID) is a significant concept for the cyber security to work properly. DID institutes and maintains a hardy program for critical digital asset (CDA) by implementing multiple security boundaries. In this work, we assign cyber security levels to a typical digital I and C system using DID concept. This work is very useful in applying the concept of DID to nuclear industry with respect to cyber security

  8. Theoretical implications of cellular immune reactions against helper lymphocytes infected by an immune system retrovirus.

    Science.gov (United States)

    Reibnegger, G; Fuchs, D; Hausen, A; Werner, E R; Dierich, M P; Wachter, H

    1987-01-01

    The breakdown of the immune system induced by the human immunodeficiency virus might be due to the active immune destruction of human immunodeficiency virus-infected helper T lymphocytes expressing viral antigens. By numerical simulation, we have studied possible consequences that a hypothetical immunodeficiency virus (IDV) may have on the cellular immune response by using a mathematical model. In this model, IDV infects CD4+ (helper) T cells and is actively synthesized by the immunologically activated helper T cells. Infected helper T cells synthesizing IDV express antigenic determinants specific for IDV and trigger a cellular immune response against themselves that is mediated by cytotoxic T cells and cytotoxic macrophages. The dynamic evolution of the model in the case of mixed-type infections with IDV and with another pathogen that evokes a cell-mediated immune response shows strong interactions between both simultaneous infections. The model might be of value to elucidate the dynamics leading to opportunistic infections. Furthermore, a pivotal role for immunological stimulation in the progressive exacerbation of the disease can be demonstrated. PMID:2959958

  9. Cocaine and MDMA Induce Cellular and Molecular Changes in Adult Neurogenic Systems: Functional Implications

    Directory of Open Access Journals (Sweden)

    Vivian Capilla-Gonzalez

    2011-06-01

    Full Text Available The capacity of the brain to generate new adult neurons is a recent discovery that challenges the old theory of an immutable adult brain. A new and fascinating field of research now focuses on this regenerative process. The two brain systems that constantly produce new adult neurons, known as the adult neurogenic systems, are the dentate gyrus (DG of the hippocampus and the lateral ventricules/olfactory bulb system. Both systems are involved in memory and learning processes. Different drugs of abuse, such as cocaine and MDMA, have been shown to produce cellular and molecular changes that affect adult neurogenesis. This review summarizes the effects that these drugs have on the adult neurogenic systems. The functional relevance of adult neurogenesis is obscured by the functions of the systems that integrate adult neurons. Therefore, we explore the effects that cocaine and MDMA produce not only on adult neurogenesis, but also on the DG and olfactory bulbs. Finally, we discuss the possible role of new adult neurons in cocaine- and MDMA-induced impairments. We conclude that, although harmful drug effects are produced at multiple physiological and anatomical levels, the specific consequences of reduced hippocampus neurogenesis are unclear and require further exploration.

  10. Investigation of Applications of SA in the Design of Dynamic Cellular Manufacturing Systems

    Directory of Open Access Journals (Sweden)

    V. Jayakumar,

    2010-08-01

    Full Text Available Manufacturing industries are under intense pressure from the increasingly competitive global marketplace. Shorter product lifecycle, time to market and diverse customer needs have challengedmanufacturers to improve the efficiency and productivity of their production activities. Manufacturing systems should be able to adjust or respond quickly to adopt necessary changes in product design and product demand without major investment. Traditional manufacturingsystems are not capable of satisfying such requirements. Although a cellular manufacturing system (CMS provides great benefits, the design of CMS is complex for real life problems. The design of such a kind of manufacturing system under dynamic production environment, with variety and demand varying between each planning horizon, requires pervasive use of a Metaheuristics such as Genetic Algorithm (GA, Simulated Annealing algorithm (SA, and Tabu Search (TS. Thedynamic cell formation (CF problem (involving the formation of a mathematical model depicting the variableproduct mix and demand across the planning horizons is known to be one of the NP-hard combinational problems. Although some optimization algorithms can find the optimal solution for small- and medium-sized problems, they have a disadvantage in that the memory and computational time requirements are extremely high, and increase exponentially, as the problem size increases. In such situations, meta-heuristics are used for exploring and exploiting the search space to obtain good solutions. In contrast to other stochastic searches, SAs in particularhave the following unique features: it does not get trapped in local minimum. Allow uphill moves controlled by parameter called temperature. Final result not dependent on initial state.These features often makes them a preferable choice over traditional heuristics. The objective of this paper is to review how the SA has been applied so far for the Design of Cellular Manufacturing

  11. Multi-flow scheduling for coordinated direct and relayed users in cellular systems

    DEFF Research Database (Denmark)

    Thai, Chan; Popovski, Petar; Kaneko, Megumi;

    2013-01-01

    There are two basic principles used in wireless network coding to design throughput-efficient schemes: (1) aggregation of communication flows and (2) interference is embraced and subsequently cancelled or mitigated. These principles inspire design of many novel multi-flow transmission (MFT) schemes....... Such are the Coordinated Direct/Relay (CDR) schemes, where each basic transmission involves two flows to a direct and a relayed user. Usage of MFT schemes as building blocks of more complex transmission schemes essentially changes the problem of scheduling, since some of the flows to be scheduled are...... coupled in a signal domain and they need to be assigned a communication resource simultaneously. In this paper we define a novel framework that can be used to analyze MFT schemes and assess the system-level gains. The framework is based on cellular wireless users with two-way traffic and it sets the basis...

  12. Fixed channel assignment in cellular communication systems considering the whole set of packed patterns

    DEFF Research Database (Denmark)

    Borges, Pedro Manuel F. C.; Vidal, Rene Victor Valqui

    2000-01-01

    This paper addresses the problem of fixed channel assignment in cellular communication systems with nonuniform traffic distribution. The objective of the channel assignment is to minimise the average blocking probability. Methods for finding a good allocation can be based on first building a number...... of sets of cochannel cells or allocation patterns and then assigning them to channels. This usually implies that only a subset of the feasible region is attainable. The approach suggested in this paper uses the concept of packed pattern, since all patterns in an optimal solution will be of that kind...... further advantage of flexibility when dealing with extensions to the problem. A neighbouring structure was used, that facilitated the calculations while still allowing for the search in the entire solution space. A summary of extensive numerical experiments is presented. The outcome is an improvement over...

  13. Designing a mathematical model for integrating dynamic cellular manufacturing into supply chain system

    Science.gov (United States)

    Aalaei, Amin; Davoudpour, Hamid

    2012-11-01

    This article presents designing a new mathematical model for integrating dynamic cellular manufacturing into supply chain system with an extensive coverage of important manufacturing features consideration of multiple plants location, multi-markets allocation, multi-period planning horizons with demand and part mix variation, machine capacity, and the main constraints are demand of markets satisfaction in each period, machine availability, machine time-capacity, worker assignment, available time of worker, production volume for each plant and the amounts allocated to each market. The aim of the proposed model is to minimize holding and outsourcing costs, inter-cell material handling cost, external transportation cost, procurement & maintenance and overhead cost of machines, setup cost, reconfiguration cost of machines installation and removal, hiring, firing and salary worker costs. Aimed to prove the potential benefits of such a design, presented an example is shown using a proposed model.

  14. Computer simulation of a cellular automata model for the immune response in a retrovirus system

    International Nuclear Information System (INIS)

    Immune response in a retrovirus system is modeled by a network of three binary cell elements to take into account some of the main functional features of T4 cells, T8 cells, and viruses. Two different intercell interactions are introduced, one of which leads to three fixed points while the other yields bistable fixed points oscillating between a healthy state and a sick state in a mean field treatment. Evolution of these cells is studied for quenched and annealed random interactions on a simple cubic lattice with a nearest neighbor interaction using inhomogenous cellular automata. Populations of T4 cells and viral cells oscillate together with damping (with constant amplitude) for annealed (quenched) interaction on increasing the value of mixing probability B from zero to a characteristic value Bca (Bcq). For higher B, the average number of T4 cells increases while that of the viral infected cells decreases monotonically on increasing B, suggesting a phase transition at Bca (Bcq)

  15. Fixed channel assignment in cellular communication systems considering the whole set of packed patterns

    DEFF Research Database (Denmark)

    Borges, Pedro Manuel F. C.; Vidal, Rene Victor Valqui

    2000-01-01

    This paper addresses the problem of fixed channel assignment in cellular communication systems with nonuniform traffic distribution. The objective of the channel assignment is to minimise the average blocking probability. Methods for finding a good allocation can be based on first building a number...... of sets of cochannel cells or allocation patterns and then assigning them to channels. This usually implies that only a subset of the feasible region is attainable. The approach suggested in this paper uses the concept of packed pattern, since all patterns in an optimal solution will be of that kind....... With a constructive method, the entire set of packed patterns is built and used in the optimisation process. The complexity (large-scale and nonlinearity) of the resulting problem suggested the use of general search procedures (local search, tabu search, simulated annealing, etc.), which have the...

  16. A cellular system that degrades misfolded proteins and protects against neurodegeneration.

    Science.gov (United States)

    Guo, Lili; Giasson, Benoit I; Glavis-Bloom, Alex; Brewer, Michael D; Shorter, James; Gitler, Aaron D; Yang, Xiaolu

    2014-07-01

    Misfolded proteins compromise cellular function and cause disease. How these proteins are detected and degraded is not well understood. Here we show that PML/TRIM19 and the SUMO-dependent ubiquitin ligase RNF4 act together to promote the degradation of misfolded proteins in the mammalian cell nucleus. PML selectively interacts with misfolded proteins through distinct substrate recognition sites and conjugates these proteins with the small ubiquitin-like modifiers (SUMOs) through its SUMO ligase activity. SUMOylated misfolded proteins are then recognized and ubiquitinated by RNF4 and are subsequently targeted for proteasomal degradation. We further show that PML deficiency exacerbates polyglutamine (polyQ) disease in a mouse model of spinocerebellar ataxia 1 (SCA1). These findings reveal a mammalian system that removes misfolded proteins through sequential SUMOylation and ubiquitination and define its role in protection against protein-misfolding diseases. PMID:24882209

  17. Hypoxia tolerance and antioxidant defense system of juvenile jumbo squids in oxygen minimum zones

    Science.gov (United States)

    Trübenbach, Katja; Teixeira, Tatiana; Diniz, Mário; Rosa, Rui

    2013-10-01

    Jumbo squid (Dosidicus gigas) is a large oceanic squid endemic off the Eastern Tropical Pacific that undertakes diel vertical migrations into mesopelagic oxygen minimum zones. One of the expected physiological effects of such migration is the generation of reactive oxygen species (ROS) at the surface, promoted by the transition between hypoxia and reoxygenation states. The aim of this study was to investigate the energy expenditure rates and the antioxidant stress strategies of juvenile D. gigas under normoxia and hypoxia, namely by quantifying oxygen consumption rates, antioxidant enzyme activities [including superoxide dismutase (SOD), catalase (CAT) and glutathione-S-transferase (GST)], heat shock protein expression (Hsp70/Hsc70), and lipid peroxidation [malondialdehyde (MDA) levels]. A high significant decrease (68%) in squid's metabolic rates was observed during hypoxia (pGST activity and MDA concentrations did not change significantly from normoxia to hypoxia (p>0.05), with the latter indicating no enhancement of lipid peroxidation (i.e. cellular damage) at the warmer and normoxic surface waters. The understanding of such physiological strategies that are linked to oxygen deprivation and reoxygenation phases may provide valuable information about how this species is quickly responding to the impacts of environmental stressors coupled with global climate change.

  18. Heavy metal induced antioxidant defense system of green microalgae and its effective role in phycoremediation of tannery effluent.

    Science.gov (United States)

    Ajayan, K V; Selvaraju, M

    2012-11-15

    Investigation of tannery effluent toxicology in green microalgae is of great importance from ecological point of view, because heavy metal has become a major contaminant in recent years. The present study determined the effect of various concentrations (0, 10, 25, 50, 75 and 100%) of heavy metals containing tannery effluent on cell growth and antioxidant defense system of two green microalgae. Treatment with effluent induced accumulation of reactive oxygen species (ROS), superoxide dismutase (SOD), catalase (CAT) and ascorbate peroxidase (APX). Lower tannery effluent concentrations increased algal growth, whereas higher concentration suppressed the growth and photosynthetic content. Both strains of the microalgae had proven effective in removing heavy metals from aqueous solutions with the highest removal efficiency being near 100% and it can be used for phycoremediation of wastewater in large scale. PMID:24261120

  19. Tracking the Dynamic Relationship between Cellular Systems and Extracellular Subproteomes in Pseudomonas aeruginosa Biofilms.

    Science.gov (United States)

    Park, Amber J; Murphy, Kathleen; Surette, Matthew D; Bandoro, Christopher; Krieger, Jonathan R; Taylor, Paul; Khursigara, Cezar M

    2015-11-01

    The transition of the opportunistic pathogen Pseudomonas aeruginosa from free-living bacteria into surface-associated biofilm communities represents a viable target for the prevention and treatment of chronic infectious disease. We have established a proteomics platform that identified 2443 and 1142 high-confidence proteins in P. aeruginosa whole cells and outer-membrane vesicles (OMVs), respectively, at three time points during biofilm development (ProteomeXchange identifier PXD002605). The analysis of cellular systems, specifically the phenazine biosynthetic pathway, demonstrates that whole-cell protein abundance correlates to end product (i.e., pyocyanin) concentrations in biofilm but not in planktonic cultures. Furthermore, increased cellular protein abundance in this pathway results in quantifiable pyocyanin in early biofilm OMVs and OMVs from both growth modes isolated at later time points. Overall, our data indicate that the OMVs being released from the surface of the biofilm whole cells have unique proteomes in comparison to their planktonic counterparts. The relative abundance of OMV proteins from various subcellular sources showed considerable differences between the two growth modes over time, supporting the existence and preferential activation of multiple OMV biogenesis mechanisms under different conditions. The consistent detection of cytoplasmic proteins in all of the OMV subproteomes challenges the notion that OMVs are composed of outer membrane and periplasmic proteins alone. Direct comparisons of outer-membrane protein abundance levels between OMVs and whole cells shows ratios that vary greatly from 1:1 and supports previous studies that advocate the specific inclusion, or "packaging", of proteins into OMVs. The quantitative analysis of packaged protein groups suggests biogenesis mechanisms that involve untethered, rather than absent, peptidoglycan-binding proteins. Collectively, individual protein and biological system analyses of biofilm OMVs

  20. Effectiveness Evaluation of Air Defense System Based on Queuing Theory%基于排队论的防空作战效能评估

    Institute of Scientific and Technical Information of China (English)

    高志华; 唐金国; 文建国; 李木易; 刘志豪; 王雷

    2012-01-01

    文章分析了防空体系对空袭目标的拦截次数计算方法,通过排队论的方法,建立了防空体系效能评估模型。最后通过仿真计算,得出影响防空体系作战效能的重要因素,为决策提供一定的理论依据。%This text analyzes the computing technology of intercepting numbers of air defense system to the goal. Through the queuing theory method, have set up the evaluation efficiency model of air defense system. Finally simulation results show the important factors of in- fluencing the fighting efficiency of air defense system, and offer certain theoretical foundation for people who making policy.

  1. The role of time delay in adaptive cellular negative feedback systems.

    Science.gov (United States)

    Lapytsko, Anastasiya; Schaber, Jörg

    2016-06-01

    Adaptation in cellular systems is often mediated by negative feedbacks, which usually come with certain time delays causing several characteristic response patterns including an overdamped response, damped or sustained oscillations. Here, we analyse generic two-dimensional delay differential equations with delayed negative feedback describing the dynamics of biochemical adaptive signal-response networks. We derive explicit thresholds and boundaries showing how time delay determines characteristic response patterns of these networks. Applying our theoretical analyses to concrete data we show that adaptation to osmotic stress in yeast is optimal in the sense of minimizing adaptation time without causing oscillatory behaviour, i.e., a critically damped response. In addition, our framework demonstrates that a slight increase of time delay in the NF-κB system might induce a switch from damped to sustained oscillatory behaviour. Thus, we demonstrate how delay differential equations can be used to explicitly study the delay in biochemical negative feedback systems. Our analysis also provides insight into how time delay may tune biological signal-response patterns and control the systems behaviour. PMID:26995333

  2. Graphical Methods for Defense Against False-data Injection Attacks on Power System State Estimation

    OpenAIRE

    Bi, Suzhi; Jun, Ying; Zhang

    2013-01-01

    The normal operation of power system relies on accurate state estimation that faithfully reflects the physical aspects of the electrical power grids. However, recent research shows that carefully synthesized false-data injection attacks can bypass the security system and introduce arbitrary errors to state estimates. In this paper, we use graphical methods to study defending mechanisms against false-data injection attacks on power system state estimation. By securing carefully selected meter ...

  3. Cellular bases of radiation-induced residual insufficiency in the haematopoietic system

    International Nuclear Information System (INIS)

    Following radiation exposure, man's survival and further well-being largely depends on the degree of damage to his heamatopietic system. Stem cells are particualarly sensitive to radiation. Over and beyond acute radiation damge, residual radiation damage is of significance since it reduces the performance of the haematopietic system and enhances the risk of leukaemia. Knowledge concerning cellular bases may be important for preventive and therapeutic measures. The measurement method presented is based on the fact that stem cells from transfused bone marrow will settle in the spleen of highly irradiated mice and be able to reconstruct the haematopietic system. Initally individual colonies can be observed which originate from a single stem cell and the proliferation of its descendants. Counting these colonies will give the number of stem cells. The reduction of the proliferation factor measured in the stem-cell quality test apparently is not due to a shift in the age structure of the stem cell compartment but to a damage which is located within a more or less substantial proportion of the stem cells themselves. This damage is the cause of stem cell descendant growth retarded on an average. It is probable that recovery observed after irradiation is brought about by less-damaged or undamaged stem cells replacing damaged ones. Initial results point to the fact that this replacement can be influenced by treatment after irradiation. (orig./MG)

  4. System Re-set: High LET Radiation or Transient Musculoskeletal Disuse Cause Lasting Changes in Oxidative Defense Pathways Within Bone

    Science.gov (United States)

    Kumar, Akhilesh; Chatterjee, A.; Alwood, Joshua S.; Dvorochkin, Natalya; Almeida, Eduardo A. C.

    2011-01-01

    Six months post-IR, there were no notable changes in skeletal expression of 84 principal genes in the p53 signaling pathway due to low dose IR (0.5Gy), HU, or both. In contrast, numerous genes relevant to oxidative stress were regulated by the treatments, typically in a direction indicative of increased oxidative stress and impaired defense. IR and HU independently reduced (between 0.46 to 0.88 fold) expression levels of Noxa1, Gpx3, Prdx2, Prdx3, and Zmynd17. Surprisingly, transient HU alone (sham-irradiated) decreased expression of several redox-related genes (Gpx1,Gstk1, Prdx1, Txnrd2), which were not affected significantly by IR alone. Irradiation increased (1.13 fold) expression of a gene responsible for production of superoxides by neutrophils (NCF2). Of interest, only combined treatment with HU and IR led to increased expression levels of Ercc2, (1.19 fold), a DNA excision repair enzyme. Differences in gene expression levels may reflect a change in gene expression on a per cell basis, a shift in the repertoire of specific cell types within the tissue, or both. Serum nitrite/nitrate levels were elevated to comparable levels (1.6-fold) due to IR, HU or both, indicative of elevated systemic nitrosyl stress. CONCLUSIONS The magnitude of changes in skeletal expression of oxidative stress-related genes six months after irradiation and/or transient unloading tended to be relatively modest (0.46-1.15 fold), whereas the p53 pathway was not affected. The finding that many different oxidative stress-related genes differed from controls at this late time point implicates a generalized impairment of oxidative defense within skeletal tissue, which coincides with both profound radiation damage to osteoprogenitors/stem cells in bone marrow and impaired remodeling of mineralized tissue.

  5. 75 FR 75549 - Defense Federal Acquisition Regulation Supplement; Business Systems-Definition and Administration...

    Science.gov (United States)

    2010-12-03

    ...-D038) in the Federal Register on January 15, 2010 (75 FR 2457). The public comment period closed March... to evaluate the adequacy of contractor business systems. Furthermore, to reduce the subjectivity...

  6. Using a Virtual Tissue Culture System to Assist Students in Understanding Life at the Cellular Level

    Science.gov (United States)

    McLauglin, Jacqueline S.; Seaquist, Stephen B.

    2008-01-01

    In every biology course ever taught in the nation's classrooms, and in every biology book ever published, students are taught about the "cell." The cell is as fundamental to biology as the atom is to chemistry. Truly, everything an organism does occurs fundamentally at the cellular level. Beyond memorizing the cellular definition, students are not…

  7. Feeding Behaviors in Cellular Slime Molds: A Microbial System To Study Competition.

    Science.gov (United States)

    Bozzone, Donna M.

    1997-01-01

    Describes a laboratory project for first-year biology students that examines competition among various cellular slime molds. After a brief introduction to the topic of competition and basic life history information about cellular slime molds, students choose a question and design original experiments to seek an answer. (Author/AIM)

  8. Cellular Decomposition Based Hybrid-Hierarchical Control Systems with Applications to Flight Management Systems

    Science.gov (United States)

    Caines, P. E.

    1999-01-01

    The work in this research project has been focused on the construction of a hierarchical hybrid control theory which is applicable to flight management systems. The motivation and underlying philosophical position for this work has been that the scale, inherent complexity and the large number of agents (aircraft) involved in an air traffic system imply that a hierarchical modelling and control methodology is required for its management and real time control. In the current work the complex discrete or continuous state space of a system with a small number of agents is aggregated in such a way that discrete (finite state machine or supervisory automaton) controlled dynamics are abstracted from the system's behaviour. High level control may then be either directly applied at this abstracted level, or, if this is in itself of significant complexity, further layers of abstractions may be created to produce a system with an acceptable degree of complexity at each level. By the nature of this construction, high level commands are necessarily realizable at lower levels in the system.

  9. Multi-modal low cost mobile indoor surveillance system on the Robust Artificial Intelligence-based Defense Electro Robot (RAIDER)

    Science.gov (United States)

    Nair, Binu M.; Diskin, Yakov; Asari, Vijayan K.

    2012-10-01

    We present an autonomous system capable of performing security check routines. The surveillance machine, the Clearpath Husky robotic platform, is equipped with three IP cameras with different orientations for the surveillance tasks of face recognition, human activity recognition, autonomous navigation and 3D reconstruction of its environment. Combining the computer vision algorithms onto a robotic machine has given birth to the Robust Artificial Intelligencebased Defense Electro-Robot (RAIDER). The end purpose of the RAIDER is to conduct a patrolling routine on a single floor of a building several times a day. As the RAIDER travels down the corridors off-line algorithms use two of the RAIDER's side mounted cameras to perform a 3D reconstruction from monocular vision technique that updates a 3D model to the most current state of the indoor environment. Using frames from the front mounted camera, positioned at the human eye level, the system performs face recognition with real time training of unknown subjects. Human activity recognition algorithm will also be implemented in which each detected person is assigned to a set of action classes picked to classify ordinary and harmful student activities in a hallway setting.The system is designed to detect changes and irregularities within an environment as well as familiarize with regular faces and actions to distinguish potentially dangerous behavior. In this paper, we present the various algorithms and their modifications which when implemented on the RAIDER serves the purpose of indoor surveillance.

  10. Scaling Laws and Design Principles for Multi-Cellular Wireless OFDMA Systems

    CERN Document Server

    Aggarwal, Rohit; Schniter, Philip

    2011-01-01

    In this paper, we consider the downlink of large-scale multi-cellular OFDMA-based networks and study performance bounds of the system as a function of the number of users $K$, the number of base-stations $B$, and the number of resource-blocks $N$. Here, a resource block is a collection of subcarriers such that all such collections, that are disjoint have associated independently fading channels. We derive novel upper and lower bounds on the sum-utility for a general spatial geometry of base stations, a truncated path loss model, and a variety of fading models (Rayleigh, Nakagami-$m$, Weibull, and LogNormal). We also establish the associated scaling laws and show that, in the special case of fixed number of resource blocks, a grid-based network of base stations, and Rayleigh-fading channels, the sum information capacity of the system scales as $\\Theta(B \\log\\log K/B)$ for extended networks, and as $O(B \\log\\log K)$ and $\\Omega(\\log \\log K)$ for dense networks. Interpreting these results, we develop some design...

  11. Kinetic Monte Carlo and cellular particle dynamics simulations of multicellular systems

    Science.gov (United States)

    Flenner, Elijah; Janosi, Lorant; Barz, Bogdan; Neagu, Adrian; Forgacs, Gabor; Kosztin, Ioan

    2012-03-01

    Computer modeling of multicellular systems has been a valuable tool for interpreting and guiding in vitro experiments relevant to embryonic morphogenesis, tumor growth, angiogenesis and, lately, structure formation following the printing of cell aggregates as bioink particles. Here we formulate two computer simulation methods: (1) a kinetic Monte Carlo (KMC) and (2) a cellular particle dynamics (CPD) method, which are capable of describing and predicting the shape evolution in time of three-dimensional multicellular systems during their biomechanical relaxation. Our work is motivated by the need of developing quantitative methods for optimizing postprinting structure formation in bioprinting-assisted tissue engineering. The KMC and CPD model parameters are determined and calibrated by using an original computational-theoretical-experimental framework applied to the fusion of two spherical cell aggregates. The two methods are used to predict the (1) formation of a toroidal structure through fusion of spherical aggregates and (2) cell sorting within an aggregate formed by two types of cells with different adhesivities.

  12. Resource Allocation for Downlink Cellular OFDMA Systems: Part II - Asymptotic Analysis and Applications

    CERN Document Server

    Ksairi, Nassar; ciblat, Phiippe; Hachem, Walid

    2008-01-01

    In a companion paper (see Resource Allocation for Downlink Cellular OFDMA systems: Part I - Optimal Allocation), we characterized the optimal resource allocation in terms of power control and sub-carrier assignment, for a downlink OFDMA system impaired by multicell interference. In our model, the network is assumed to be one dimensional (linear) for the sake of analysis. We also assume that a certain part of the available bandwidth is likely to be reused by different base stations and that the other part of the bandwidth is shared in an orthogonal way between the different base stations. The optimal resource allocation characterized in Part I is obtained by minimizing the total power spent by the network under the constraint that all users rate requirements in nats/s/Hz are satisfied. It is worth noting that when optimal resource allocation is used, any user receives data either in the reused bandwidth or in the protected bandwidth, but not in both (except for at most one pivot user in each cell). In the pres...

  13. On Intercell Interference and Its Cancellation in Cellular Multicarrier CDMA Systems

    Directory of Open Access Journals (Sweden)

    Plass Simon

    2008-01-01

    Full Text Available Abstract The handling of intercell interference at the cell border area is a strong demand in future communication systems to guarantee efficient use of the available bandwidth. Therefore, this paper focuses on the application of iterative intercell interference cancellation schemes in cellular multicarrier code division multiple access (MC-CDMA systems at the receiver side for the downlink. First, the influence of the interfering base stations to the total intercell interference is investigated. Then, different concepts for intercell interference cancellation are described and investigated for scenarios with several interfering cells. The first approach is based on the use of the hard decision of the demodulator to reconstruct the received signals. This does not require the higher amount of complexity compared to the second approach which is based on the use of the more reliable soft values from the decoding process. Furthermore, the extrinsic information as a reliability measure of this soft iterative cancellation process is investigated in more detail based on the geographical position of the mobile terminal. Both approaches show significant performance gains in the severe cell border area. With the soft intercell interference cancellation scheme, it is possible to reach the single-user bound. Therefore, the intercell interference can be almost eliminated.

  14. Multi-tier Network Performance Analysis using a Shotgun Cellular System

    CERN Document Server

    Madhusudhanan, Prasanna; Youjian,; Liu,; Brown, Timothy X; Baker, Kenneth R

    2011-01-01

    This paper studies the carrier-to-interference ratio (CIR) and carrier-to-interference-plus-noise ratio (CINR) performance at the mobile station (MS) within a multi-tier network composed of M tiers of wireless networks, with each tier modeled as the homogeneous n-dimensional (n-D, n=1,2, and 3) shotgun cellular system, where the base station (BS) distribution is given by the homogeneous Poisson point process in n-D. The CIR and CINR at the MS in a single tier network are thoroughly analyzed to simplify the analysis of the multi-tier network. For the multi-tier network with given system parameters, the following are the main results of this paper: (1) semi-analytical expressions for the tail probabilities of CIR and CINR; (2) a closed form expression for the tail probability of CIR in the range [1,Infinity); (3) a closed form expression for the tail probability of an approximation to CIR in the entire range [0,Infinity); (4) a lookup table based approach for obtaining the tail probability of CINR, and (5) the ...

  15. OFDM AF Variable Gain Relay System for the Next Generation Mobile Cellular

    Directory of Open Access Journals (Sweden)

    E. Kocan

    2012-06-01

    Full Text Available In this paper we present analytical performance evaluation of a dual-hop OFDM amplify-andforward (AF variable gain (VG relay system implementing ordered subcarrier mapping (SCM at the relay station (R, considered to be a very interesting solution for the next generation mobile cellular networks. A scenario with no direct communication between the source of information (S and destination terminal (D, with the Rayleigh fading statistics on both hops is assumed. A closed form analytical expression for the bit error rate (BER performance of the considered system with DPSK modulation is derived, while for its ergodic capacity performance, a tight upper bound expression is obtained. The accuracy of the undertaken analytical approach is confirmed through comparison with simulation results. It is shown that significant capacity enhancement can be achieved through SCM implementation at R, for all the signal-to-noise ratio (SNR values on both hops, but especially in the region of small SNRs on hops. BER analysis reveals that in the region of small and medium average SNRs on both hops BER performance may also be improved with SCM at R station.

  16. Near-Earth Objects: Targets for Future Human Exploration, Solar System Science, and Planetary Defense

    Science.gov (United States)

    Abell, Paul A.

    2011-01-01

    Human exploration of near-Earth objects (NEOs) beginning circa 2025 - 2030 is one of the stated objectives of U.S. National Space Policy. Piloted missions to these bodies would further development of deep space mission systems and technologies, obtain better understanding of the origin and evolution of our Solar System, and support research for asteroid deflection and hazard mitigation strategies. This presentation will discuss some of the physical characteristics of NEOs and review some of the current plans for NEO research and exploration from both a human and robotic mission perspective.

  17. The DEFENSE (debris Flows triggEred by storms - nowcasting system): An early warning system for torrential processes by radar storm tracking using a Geographic Information System (GIS)

    Science.gov (United States)

    Tiranti, Davide; Cremonini, Roberto; Marco, Federica; Gaeta, Armando Riccardo; Barbero, Secondo

    2014-09-01

    Debris flows, responsible for economic losses and occasionally casualties in the alpine region, are mainly triggered by heavy rains characterized by hourly peaks of varying intensity, depending on the features of the basin under consideration. By integrating a recent classification of alpine basins with the radar storm tracking method, an innovative early warning system called DEFENSE (DEbris Flows triggEred by storms - Nowcasting SystEm) was developed using a Geographical Information System (GIS). Alpine catchments were classified into three main classes based on the weathering capacity of the bedrock into clay or clay-like minerals, the amount of which, in unconsolidated material, directly influences the debris flow rheology, and thus the sedimentary processes, the alluvial fan architecture, as well as the triggering frequency and seasonal occurrence probability of debris flows. Storms were identified and tracked by processing weather radar observations; subsequently, rainfall intensities and storm severity were estimated over each classified basin. Due to rainfall threshold values determined for each basin class, based on statistical analysis of historical records, an automatic corresponding warning could be issued to municipalities.

  18. In defense of unified health system: discourses of health professionals, municipal counselors and aldermen

    Directory of Open Access Journals (Sweden)

    Karly Garcia Delamuta

    2015-03-01

    Full Text Available The objective of this study was to evaluate the perceptions of municipal health counselors, primary care professionals and aldermen about the Unified Health System and the Brazilian Primary Care Policy. From these, we intend to analyze their involvement to improve the system and verify participation in projects that foster discussions about the challenges of this issue. The investigation took a qualitative approach, the data being collected through 28 semi-structured interviews conducted between November and December 2010 in Londrina-PR. Between the interviewed groups, it becomes apparent that health professionals have better conceptual approach of public health policies. However, all groups demonstrate misconceptions and distance for the principles and guidelines of the Unified Health System, as well as provisions of the Brazilian Primary Care Policy. The findings pointed indicate focus on disease, prioritization of medical consultations and greater value of hospital structures. Although conceptualized with misconceptions, limitations are noted at the public health services. However, the proposals to change the frame remain with distorted connotations. In these groups no practical actions or projects were found to improve the public health scenario. It is concluded the need for ownership of theoretical knowledge about policies involving health organization, by stakeholders, to change the paradigms of the traditional model to the Primary Health care become valued and understood as form of organization of the system.

  19. Hetero-cellular prototyping by synchronized multi-material bioprinting for rotary cell culture system.

    Science.gov (United States)

    Snyder, Jessica; Son, Ae Rin; Hamid, Qudus; Wu, Honglu; Sun, Wei

    2016-03-01

    Bottom-up tissue engineering requires methodological progress of biofabrication to capture key design facets of anatomical arrangements across micro, meso and macro-scales. The diffusive mass transfer properties necessary to elicit stability and functionality require hetero-typic contact, cell-to-cell signaling and uniform nutrient diffusion. Bioprinting techniques successfully build mathematically defined porous architecture to diminish resistance to mass transfer. Current limitations of bioprinted cell assemblies include poor micro-scale formability of cell-laden soft gels and asymmetrical macro-scale diffusion through 3D volumes. The objective of this work is to engineer a synchronized multi-material bioprinter (SMMB) system which improves the resolution and expands the capability of existing bioprinting systems by packaging multiple cell types in heterotypic arrays prior to deposition. This unit cell approach to arranging multiple cell-laden solutions is integrated with a motion system to print heterogeneous filaments as tissue engineered scaffolds and nanoliter droplets. The set of SMMB process parameters control the geometric arrangement of the combined flow's internal features and constituent material's volume fractions. SMMB printed hepatocyte-endothelial laden 200 nl droplets are cultured in a rotary cell culture system (RCCS) to study the effect of microgravity on an in vitro model of the human hepatic lobule. RCCS conditioning for 48 h increased hepatocyte cytoplasm diameter 2 μm, increased metabolic rate, and decreased drug half-life. SMMB hetero-cellular models present a 10-fold increase in metabolic rate, compared to SMMB mono-culture models. Improved bioprinting resolution due to process control of cell-laden matrix packaging as well as nanoliter droplet printing capability identify SMMB as a viable technique to improve in vitro model efficacy. PMID:26759993

  20. Implementation of a cellular neural network-based segmentation algorithm on the bio-inspired vision system

    Science.gov (United States)

    Karabiber, Fethullah; Grassi, Giuseppe; Vecchio, Pietro; Arik, Sabri; Yalcin, M. Erhan

    2011-01-01

    Based on the cellular neural network (CNN) paradigm, the bio-inspired (bi-i) cellular vision system is a computing platform consisting of state-of-the-art sensing, cellular sensing-processing and digital signal processing. This paper presents the implementation of a novel CNN-based segmentation algorithm onto the bi-i system. The experimental results, carried out for different benchmark video sequences, highlight the feasibility of the approach, which provides a frame rate of about 26 frame/sec. Comparisons with existing CNN-based methods show that, even though these methods are from two to six times faster than the proposed one, the conceived approach is more accurate and, consequently, represents a satisfying trade-off between real-time requirements and accuracy.

  1. Dissertation Defense: Computational Fluid Dynamics Uncertainty Analysis for Payload Fairing Spacecraft Environmental Control Systems

    Science.gov (United States)

    Groves, Curtis Edward

    2014-01-01

    Spacecraft thermal protection systems are at risk of being damaged due to airflow produced from Environmental Control Systems. There are inherent uncertainties and errors associated with using Computational Fluid Dynamics to predict the airflow field around a spacecraft from the Environmental Control System. This paper describes an approach to quantify the uncertainty in using Computational Fluid Dynamics to predict airflow speeds around an encapsulated spacecraft without the use of test data. Quantifying the uncertainty in analytical predictions is imperative to the success of any simulation-based product. The method could provide an alternative to traditional validation by test only mentality. This method could be extended to other disciplines and has potential to provide uncertainty for any numerical simulation, thus lowering the cost of performing these verifications while increasing the confidence in those predictions.Spacecraft requirements can include a maximum airflow speed to protect delicate instruments during ground processing. Computational Fluid Dynamics can be used to verify these requirements; however, the model must be validated by test data. This research includes the following three objectives and methods. Objective one is develop, model, and perform a Computational Fluid Dynamics analysis of three (3) generic, non-proprietary, environmental control systems and spacecraft configurations. Several commercially available and open source solvers have the capability to model the turbulent, highly three-dimensional, incompressible flow regime. The proposed method uses FLUENT, STARCCM+, and OPENFOAM. Objective two is to perform an uncertainty analysis of the Computational Fluid Dynamics model using the methodology found in Comprehensive Approach to Verification and Validation of Computational Fluid Dynamics Simulations. This method requires three separate grids and solutions, which quantify the error bars around Computational Fluid Dynamics predictions

  2. Dissertation Defense Computational Fluid Dynamics Uncertainty Analysis for Payload Fairing Spacecraft Environmental Control Systems

    Science.gov (United States)

    Groves, Curtis Edward

    2014-01-01

    Spacecraft thermal protection systems are at risk of being damaged due to airflow produced from Environmental Control Systems. There are inherent uncertainties and errors associated with using Computational Fluid Dynamics to predict the airflow field around a spacecraft from the Environmental Control System. This paper describes an approach to quantify the uncertainty in using Computational Fluid Dynamics to predict airflow speeds around an encapsulated spacecraft without the use of test data. Quantifying the uncertainty in analytical predictions is imperative to the success of any simulation-based product. The method could provide an alternative to traditional "validation by test only" mentality. This method could be extended to other disciplines and has potential to provide uncertainty for any numerical simulation, thus lowering the cost of performing these verifications while increasing the confidence in those predictions. Spacecraft requirements can include a maximum airflow speed to protect delicate instruments during ground processing. Computational Fluid Dynamics can be used to verify these requirements; however, the model must be validated by test data. This research includes the following three objectives and methods. Objective one is develop, model, and perform a Computational Fluid Dynamics analysis of three (3) generic, non-proprietary, environmental control systems and spacecraft configurations. Several commercially available and open source solvers have the capability to model the turbulent, highly three-dimensional, incompressible flow regime. The proposed method uses FLUENT, STARCCM+, and OPENFOAM. Objective two is to perform an uncertainty analysis of the Computational Fluid Dynamics model using the methodology found in "Comprehensive Approach to Verification and Validation of Computational Fluid Dynamics Simulations". This method requires three separate grids and solutions, which quantify the error bars around Computational Fluid Dynamics

  3. A Systems Biology Approach to the Coordination of Defensive and Offensive Molecular Mechanisms in the Innate and Adaptive Host-Pathogen Interaction Networks.

    Science.gov (United States)

    Wu, Chia-Chou; Chen, Bor-Sen

    2016-01-01

    Infected zebrafish coordinates defensive and offensive molecular mechanisms in response to Candida albicans infections, and invasive C. albicans coordinates corresponding molecular mechanisms to interact with the host. However, knowledge of the ensuing infection-activated signaling networks in both host and pathogen and their interspecific crosstalk during the innate and adaptive phases of the infection processes remains incomplete. In the present study, dynamic network modeling, protein interaction databases, and dual transcriptome data from zebrafish and C. albicans during infection were used to infer infection-activated host-pathogen dynamic interaction networks. The consideration of host-pathogen dynamic interaction systems as innate and adaptive loops and subsequent comparisons of inferred innate and adaptive networks indicated previously unrecognized crosstalk between known pathways and suggested roles of immunological memory in the coordination of host defensive and offensive molecular mechanisms to achieve specific and powerful defense against pathogens. Moreover, pathogens enhance intraspecific crosstalk and abrogate host apoptosis to accommodate enhanced host defense mechanisms during the adaptive phase. Accordingly, links between physiological phenomena and changes in the coordination of defensive and offensive molecular mechanisms highlight the importance of host-pathogen molecular interaction networks, and consequent inferences of the host-pathogen relationship could be translated into biomedical applications. PMID:26881892

  4. A low cost maritime control aircraft-ship-weapons system. [antiship missile defense

    Science.gov (United States)

    Fluk, H.

    1981-01-01

    It is pointed out that the long-range antiship standoff missile is emerging as the foremost threat on the seas. Delivered by high speed bombers, surface ships, and submarines, a missile attack can be mounted against selected targets from any point on the compass. An investigation is conducted regarding the configuration of a system which could most efficiently identify and destroy standoff threats before they launch their weapons. It is found that by using ships for carrying and launching missiles, and employing aircraft with a powerful radar only for search and missile directing operations, aircraft cost and weight can be greatly reduced. The employment of V/STOL aircraft in preference to other types of aircraft makes it possible to use ships of smaller size for carrying the aircraft. However, in order to obtain an all-weather operational capability for the system, ships are selected which are still big enough to display the required stability in heavy seas.

  5. Near-Earth Objects: Targets for Future Human Exploration, Solar System Science, Resource Utilization, and Planetary Defense

    Science.gov (United States)

    Abell, Paul A.

    2011-01-01

    U.S. President Obama stated on April 15, 2010 that the next goal for human spaceflight will be to send human beings to a near-Earth asteroid by 2025. Given this direction from the White House, NASA has been involved in studying various strategies for near-Earth object (NEO) exploration in order to follow U.S. Space Exploration Policy. This mission would be the first human expedition to an interplanetary body beyond the Earth-Moon system and would prove useful for testing technologies required for human missions to Mars and other Solar System destinations. Missions to NEOs would undoubtedly provide a great deal of technical and engineering data on spacecraft operations for future human space exploration while conducting in-depth scientific investigations of these primitive objects. In addition, the resulting scientific investigations would refine designs for future extraterrestrial resource extraction and utilization, and assist in the development of hazard mitigation techniques for planetary defense. This presentation will discuss some of the physical characteristics of NEOs and review some of the current plans for NEO research and exploration from both a human and robotic mission perspective.

  6. Active cyber defense: enhancing national cyber defense

    OpenAIRE

    Wong, Tiong Pern.

    2011-01-01

    With increased dependency on the Internet, cyber attacks are fast becoming an attractive option for state adversaries, in part because of the ease of hiding one's identity. In response, governments around the world are taking measures to improve their national cyber defenses. However, these defenses, which are generally passive in nature, have been insufficient to address the threat. This thesis explores the possibility of employing active cyber defenses to improve cyber defenses at the na...

  7. The Regional Dimension of Romania's Participation to the Anti-Missile Defense System

    Directory of Open Access Journals (Sweden)

    Florin Iftode

    2015-08-01

    Full Text Available The current security environment is characterized by a high degree of instability and unpredictability, through the manifestation of new risks and threats, especially those with asymmetrical nature, by redefining the relations between the great powers, and increasing freedom of action of the regional actors (state and non-state. At the international level, the end of the Cold War has led to a fragmentation of efforts in the security field, with direct implications on the need for further efforts of the international community coordination. In this era of profound change and transformation it is not possible the adoption of an isolationist policy of security, but only in a broader context of interdependencies and relationships between general trends, threats or challenges specific to a particular area or geographic areas. Swift operationalization of the anti-missile system will strengthen the national security both directly, through the protection it provides for the entire national territory, and indirectly, as the system will have a deterrent important role, able to mitigate risks of possible attacks by ballistic missiles. The role of deterrence will remove the risk of attack against any Allied territory on which, by virtue of membership in NATO and obligations involved according to article 5 of the Washington Treaty, Romania has also responsibilities.

  8. In the presence of danger:the extracellular matrix defensive response to central nervous system injury

    Institute of Scientific and Technical Information of China (English)

    Lyn B. Jakeman; Kent E. Williams; Bryan Brautigam

    2014-01-01

    Glial cells in the central nervous system (CNS) contribute to formation of the extracellular matrix, which provides adhesive sites, signaling molecules, and a diffusion barrier to enhance efifcient neurotransmission and axon potential propagation. In the normal adult CNS, the extracellular matrix (ECM) is relatively stable except in selected regions characterized by dynamic remodel-ing. However, after trauma such as a spinal cord injury or cortical contusion, the lesion epicenter becomes a focus of acute neuroinlfammation. The activation of the surrounding glial cells leads to a dramatic change in the composition of the ECM at the edges of the lesion, creating a perile-sion environment dominated by growth inhibitory molecules and restoration of the peripheral/central nervous system border. An advantage of this response is to limit the invasion of damaging cells and diffusion of toxic molecules into the spared tissue regions, but this occurs at the cost of inhibiting migration of endogenous repair cells and preventing axonal regrowth. The following review was prepared by reading and discussing over 200 research articles in the ifeld published in PubMed and selecting those with signiifcant impact and/or controversial points. This article highlights structural and functional features of the normal adult CNS ECM and then focuses on the reactions of glial cells and changes in the perilesion border that occur following spinal cord or contusive brain injury. Current research strategies directed at modifying the inhibitory perile-sion microenvironment without eliminating the protective functions of glial cell activation are discussed.

  9. Effects of parasite pressure on parasite mortality and reproductive output in a rodent-flea system: inferring host defense trade-offs.

    Science.gov (United States)

    Warburton, Elizabeth M; Kam, Michael; Bar-Shira, Enav; Friedman, Aharon; Khokhlova, Irina S; Koren, Lee; Asfur, Mustafa; Geffen, Eli; Kiefer, Daniel; Krasnov, Boris R; Degen, A Allan

    2016-09-01

    Evaluating host resistance via parasite fitness helps place host-parasite relationships within evolutionary and ecological contexts; however, few studies consider both these processes simultaneously. We investigated how different levels of parasite pressure affect parasite mortality and reproductive success in relationship to host defense efforts, using the rodent Gerbillus nanus and the flea Xenopsylla conformis as a host-parasite system. Fifteen immune-naïve male rodents were infested with 20, 50, or 100 fleas for four weeks. During this time number of new imagoes produced per adult flea (our flea reproductive output metric), flea mortality, and change in circulating anti-flea immunoglobulin G (our measure of adaptive immune defense) were monitored. Three hypotheses guided this work: (1) increasing parasite pressure would heighten host defenses; (2) parasite mortality would increase and parasite reproductive output would decrease with increasing investment in host defense; and (3) hosts under high parasite pressure could invest in behavioral and/or immune responses. We predicted that at high infestation levels (a) parasite mortality would increase; (b) flea reproductive output per individual would decrease; and (c) host circulating anti-flea antibody levels would increase. The hypotheses were partially supported. Flea mortality significantly increased and flea reproductive output significantly decreased as flea pressure increased. Host adaptive immune defense did not significantly change with increasing flea pressure. Therefore, we inferred that investment in host behavioral defense, either alone or in combination with density-dependent effects, may be more efficient at increasing flea mortality and decreasing flea reproductive output than antibody production during initial infestation in this system. PMID:27130319

  10. Designing and implementing a regional urban modeling system using the SLEUTH cellular urban model

    Science.gov (United States)

    Jantz, C.A.; Goetz, S.J.; Donato, D.; Claggett, P.

    2010-01-01

    This paper presents a fine-scale (30 meter resolution) regional land cover modeling system, based on the SLEUTH cellular automata model, that was developed for a 257000 km2 area comprising the Chesapeake Bay drainage basin in the eastern United States. As part of this effort, we developed a new version of the SLEUTH model (SLEUTH-3r), which introduces new functionality and fit metrics that substantially increase the performance and applicability of the model. In addition, we developed methods that expand the capability of SLEUTH to incorporate economic, cultural and policy information, opening up new avenues for the integration of SLEUTH with other land-change models. SLEUTH-3r is also more computationally efficient (by a factor of 5) and uses less memory (reduced 65%) than the original software. With the new version of SLEUTH, we were able to achieve high accuracies at both the aggregate level of 15 sub-regional modeling units and at finer scales. We present forecasts to 2030 of urban development under a current trends scenario across the entire Chesapeake Bay drainage basin, and three alternative scenarios for a sub-region within the Chesapeake Bay watershed to illustrate the new ability of SLEUTH-3r to generate forecasts across a broad range of conditions. ?? 2009 Elsevier Ltd.

  11. An archived multi-objective simulated annealing for a dynamic cellular manufacturing system

    Science.gov (United States)

    Shirazi, Hossein; Kia, Reza; Javadian, Nikbakhsh; Tavakkoli-Moghaddam, Reza

    2014-05-01

    To design a group layout of a cellular manufacturing system (CMS) in a dynamic environment, a multi-objective mixed-integer non-linear programming model is developed. The model integrates cell formation, group layout and production planning (PP) as three interrelated decisions involved in the design of a CMS. This paper provides an extensive coverage of important manufacturing features used in the design of CMSs and enhances the flexibility of an existing model in handling the fluctuations of part demands more economically by adding machine depot and PP decisions. Two conflicting objectives to be minimized are the total costs and the imbalance of workload among cells. As the considered objectives in this model are in conflict with each other, an archived multi-objective simulated annealing (AMOSA) algorithm is designed to find Pareto-optimal solutions. Matrix-based solution representation, a heuristic procedure generating an initial and feasible solution and efficient mutation operators are the advantages of the designed AMOSA. To demonstrate the efficiency of the proposed algorithm, the performance of AMOSA is compared with an exact algorithm (i.e., ∈-constraint method) solved by the GAMS software and a well-known evolutionary algorithm, namely NSGA-II for some randomly generated problems based on some comparison metrics. The obtained results show that the designed AMOSA can obtain satisfactory solutions for the multi-objective model.

  12. A versatile transreplication-based system to identify cellular proteins involved in geminivirus replication.

    Science.gov (United States)

    Morilla, Gabriel; Castillo, Araceli G; Preiss, Werner; Jeske, Holger; Bejarano, Eduardo R

    2006-04-01

    A versatile green fluorescent protein (GFP) expression cassette containing the replication origins of the monopartite begomovirus Tomato yellow leaf curl Sardinia virus (TYLCSV) is described. Transgenic Nicotiana benthamiana plants containing one copy of the cassette stably integrated into their genome were superinfected with TYLCSV, which mobilized and replicated the cassette as an episomal replicon. The expression of the reporter gene (the GFP gene) was thereby modified. Whereas GFP fluorescence was dimmed in the intercostal areas, an increase of green fluorescence in veins of all leaves placed above the inoculation site, as well as in transport tissues of roots and stems, was observed. The release of episomal trans replicons from the transgene and the increase in GFP expression were dependent on the cognate geminiviral replication-associated protein (Rep) and required interaction between Rep and the intergenic region of TYLCSV. This expression system is able to monitor the replication status of TYLCSV in plants, as induction of GFP expression is only produced in those tissues where Rep is present. To further confirm this notion, the expression of a host factor required for geminivirus replication, the proliferating cellular nuclear antigen (PCNA) was transiently silenced. Inhibition of PCNA prevented GFP induction in veins and reduced viral DNA. We propose that these plants could be widely used to easily identify host factors required for geminivirus replication by virus-induced gene silencing. PMID:16537630

  13. Computer simulation of a cellular automata model for the immune response in a retrovirus system

    Science.gov (United States)

    Pandey, R. B.

    1989-02-01

    Immune response in a retrovirus system is modeled by a network of three binary cell elements to take into account some of the main functional features of T4 cells, T8 cells, and viruses. Two different intercell interactions are introduced, one of which leads to three fixed points while the other yields bistable fixed points oscillating between a healthy state and a sick state in a mean field treatment. Evolution of these cells is studied for quenched and annealed random interactions on a simple cubic lattice with a nearest neighbor interaction using inhomogenous cellular automata. Populations of T4 cells and viral cells oscillate together with damping (with constant amplitude) for annealed (quenched) interaction on increasing the value of mixing probability B from zero to a characteristic value B ca ( B cq). For higher B, the average number of T4 cells increases while that of the viral infected cells decreases monotonically on increasing B, suggesting a phase transition at B ca ( B cq).

  14. The effect of an autologous cellular gel-matrix integrated implant system on wound healing

    Directory of Open Access Journals (Sweden)

    Morales Patricio

    2010-06-01

    Full Text Available Abstract Background This manuscript reports the production and preclinical studies to examine the tolerance and efficacy of an autologous cellular gel-matrix integrated implant system (IIS aimed to treat full-thickness skin lesions. Methods The best concentration of fibrinogen and thrombin was experimentally determined by employing 28 formula ratios of thrombin and fibrinogen and checking clot formation and apparent stability. IIS was formed by integrating skin cells by means of the in situ gelification of fibrin into a porous crosslinked scaffold composed of chitosan, gelatin and hyaluronic acid. The in vitro cell proliferation within the IIS was examined by the MTT assay and PCNA expression. An experimental rabbit model consisting of six circular lesions was utilized to test each of the components of the IIS. Then, the IIS was utilized in an animal model to cover a 35% body surface full thickness lesion. Results The preclinical assays in rabbits demonstrated that the IIS was well tolerated and also that IIS-treated rabbit with lesions of 35% of their body surface, exhibited a better survival rate (p = 0,06. Conclusion IIS should be further studied as a new wound dressing which shows promising properties, being the most remarkable its good biological tolerance and cell growth promotion properties.

  15. A multi-objective model for designing a group layout of a dynamic cellular manufacturing system

    Science.gov (United States)

    Kia, Reza; Shirazi, Hossein; Javadian, Nikbakhsh; Tavakkoli-Moghaddam, Reza

    2013-04-01

    This paper presents a multi-objective mixed-integer nonlinear programming model to design a group layout of a cellular manufacturing system in a dynamic environment, in which the number of cells to be formed is variable. Cell formation (CF) and group layout (GL) are concurrently made in a dynamic environment by the integrated model, which incorporates with an extensive coverage of important manufacturing features used in the design of CMSs. Additionally, there are some features that make the presented model different from the previous studies. These features include the following: (1) the variable number of cells, (2) the integrated CF and GL decisions in a dynamic environment by a multi-objective mathematical model, and (3) two conflicting objectives that minimize the total costs (i.e., costs of intra and inter-cell material handling, machine relocation, purchasing new machines, machine overhead, machine processing, and forming cells) and minimize the imbalance of workload among cells. Furthermore, the presented model considers some limitations, such as machine capability, machine capacity, part demands satisfaction, cell size, material flow conservation, and location assignment. Four numerical examples are solved by the GAMS software to illustrate the promising results obtained by the incorporated features.

  16. Space experiment "Cellular Responses to Radiation in Space (CellRad)": Hardware and biological system tests.

    Science.gov (United States)

    Hellweg, Christine E; Dilruba, Shahana; Adrian, Astrid; Feles, Sebastian; Schmitz, Claudia; Berger, Thomas; Przybyla, Bartos; Briganti, Luca; Franz, Markus; Segerer, Jürgen; Spitta, Luis F; Henschenmacher, Bernd; Konda, Bikash; Diegeler, Sebastian; Baumstark-Khan, Christa; Panitz, Corinna; Reitz, Günther

    2015-11-01

    One factor contributing to the high uncertainty in radiation risk assessment for long-term space missions is the insufficient knowledge about possible interactions of radiation with other spaceflight environmental factors. Such factors, e.g. microgravity, have to be considered as possibly additive or even synergistic factors in cancerogenesis. Regarding the effects of microgravity on signal transduction, it cannot be excluded that microgravity alters the cellular response to cosmic radiation, which comprises a complex network of signaling pathways. The purpose of the experiment "Cellular Responses to Radiation in Space" (CellRad, formerly CERASP) is to study the effects of combined exposure to microgravity, radiation and general space flight conditions on mammalian cells, in particular Human Embryonic Kidney (HEK) cells that are stably transfected with different plasmids allowing monitoring of proliferation and the Nuclear Factor κB (NF-κB) pathway by means of fluorescent proteins. The cells will be seeded on ground in multiwell plate units (MPUs), transported to the ISS, and irradiated by an artificial radiation source after an adaptation period at 0 × g and 1 × g. After different incubation periods, the cells will be fixed by pumping a formaldehyde solution into the MPUs. Ground control samples will be treated in the same way. For implementation of CellRad in the Biolab on the International Space Station (ISS), tests of the hardware and the biological systems were performed. The sequence of different steps in MPU fabrication (cutting, drilling, cleaning, growth surface coating, and sterilization) was optimized in order to reach full biocompatibility. Different coatings of the foil used as growth surface revealed that coating with 0.1 mg/ml poly-D-lysine supports cell attachment better than collagen type I. The tests of prototype hardware (Science Model) proved its full functionality for automated medium change, irradiation and fixation of cells. Exposure of

  17. 75Se-release: A short and long term assay system for cellular cytotoxicity

    International Nuclear Information System (INIS)

    The γ-emitting aminoacid 75Se-selenomethionine (75SeM) was examined as a target cell label in cytotoxic assays. It was efficiently taken up by activated, intensely metabolizing cells of various types but hardly at all by resting or low-metabolizing cells. Culturing activated cells in methionine-deficient medium with 3-5μCi75SeM/ml for 18-22 h usually resulted in an uptake of 3-20 cpm/cell which was 3-200 times that of 51Cr marked cells. 75SeM-labelled cells kept in medium at ambient temperature or at 370C maintained a high radioactivity per cell and a viability above 85% for at least 72 h without significant increase in spontaneous isotope release or loss in sensitivity in subsequent cytotoxic tests. 75Se-labelled material released from target cells was not reutilized by unlabelled lymphoid cells. Provided the cells were carefully washed after labelling and kept in optimal culture conditions, the reasonably low baseline release in the medium control allowed performance of long-term assays of up to 54 h. However, strong cytotoxic reactions could cause over 50% specific 75Se-release within 5 h. With constant amounts of effector cells, identical or even higher specific releases were obtained on 6 x 102 targets as compared to 1 x 104 targets/well. Thus, the 75Se-release assay offers a single monitoring system suitable for short and long term cytotoxic reactions on a microscale, using 1 x 103 or less targets/well. Its sensitivity permits evaluation of strong and weak reactions as well as early and delayed onser cytotoxicity. In addition with a γ-spectrometer the radioactivity of 75Se can easily be distinguished from that of 51Cr. Due to this, and an improved method for 51Cr labelling of cells, a double γ-labelling of cellular proteins is available which provides new possibilities for monitoring cellular interactions in short and long term tests. (orig./MG)

  18. ABC transporters and xenobiotic defense systems in early life stages of rainbow trout (Oncorhynchus mykiss).

    Science.gov (United States)

    Kropf, Christian; Segner, Helmut; Fent, Karl

    2016-01-01

    Embryos of oviparous fish, in contrast to (ovo) viviparous species, develop in the aquatic environment, and therefore need solute transport systems at their body surfaces for maintaining internal homeostasis and defending against potentially harmful substances. We hypothesized that solute transporters undergo changes in tissue distribution from the embryo to the larval stage. We therefore studied the mRNA profiles of eight ABC transporters (abcb1a, abcb1b, abcc1, abcc2, abcc3, abcc4, abcc5, abcg2) and three solute carriers (oatp1d, putative oatp2 putative, mate1) in different body regions (head, yolk sac epithelium, abdominal viscera, skin/muscles) of developing rainbow trout. Additionally, we investigated mRNA levels of phase I (cyp1a, cyp3a) and phase II (gstp, putative ugt1, putative ugt2) biotransformation enzymes. The study covered the developmental period from the eleuthero-embryo stage to the first-feeding larval stage (1-20days post-hatch, dph). At 1dph, transcripts of abcc2, abcc4, abcg2, cyp3a, gstp, putative mate1, and putative oatp2 occurred primarily in the yolk sac epithelium, whereas at later stages expression of these genes was predominantly observed in the abdominal viscera. The functional activity of ABC transporters in fish early life stages was assessed by rhodamine B accumulation assays. Finally, we investigated the potential impact of xenobiotics (clotrimazole, clofibric acid) on the ABC and biotransformation systems of trout early life stages. While clofibric acid had no effect, clotrimazole lead to an increased rhodamine B accumulation. The results provide evidence that the transition from the eleuthero-embryo to the larval stage is accompanied by a major alteration in tissue expression of ABC transporters. PMID:26945521

  19. Cellular respiration: replicating in vivo systems biology for in vitro exploration of human exposome, microbiome, and disease pathogenesis biomarkers

    Science.gov (United States)

    This editorial develops a philosophy for expanding the scope of Journal of Breath Research (JBR) into the realm of cellular level study, and links certain topics back to more traditional systemic research for understanding human health based on exhaled breath constituents. The ex...

  20. Self-synthesizing transposons: unexpected key players in the evolution of viruses and defense systems.

    Science.gov (United States)

    Krupovic, Mart; Koonin, Eugene V

    2016-06-01

    Self-synthesizing transposons are the largest known transposable elements that encode their own DNA polymerases (DNAP). The Polinton/Maverick family of self-synthesizing transposons is widespread in eukaryotes and abundant in the genomes of some protists. In addition to the DNAP and a retrovirus-like integrase, most of the polintons encode homologs of the major and minor jelly-roll capsid proteins, DNA-packaging ATPase and capsid maturation protease. Therefore, polintons are predicted to alternate between the transposon and viral lifestyles although virion formation remains to be demonstrated. Polintons are related to a group of eukaryotic viruses known as virophages that parasitize on giant viruses of the family Mimiviridae and another recently identified putative family of polinton-like viruses (PLV) predicted to lead a similar, dual life style. Comparative genomic analysis of polintons, virophages, PLV and the other viruses with double-stranded (ds)DNA genomes infecting eukaryotes and prokaryotes suggests that the polintons evolved from bacterial tectiviruses and could have been the ancestors of a broad range of eukaryotic viruses including adenoviruses and members of the proposed order 'Megavirales' as well as linear cytoplasmic plasmids. Recently, a group of predicted self-synthesizing transposons was discovered also in prokaryotes. These elements, denoted casposons, encode a DNAP and a homolog of the CRISPR-associated Cas1 endonuclease that has an integrase activity but no capsid proteins. Thus, unlike polintons, casposons appear to be limited to the transposon life style although they could have evolved from viruses. The casposons are thought to have played a pivotal role in the origin of the prokaryotic adaptive immunity, giving rise to the adaptation module of the CRISPR-Cas systems. PMID:26836982

  1. Changes in Antioxidant Defense System Using Different Lipid Emulsions in Parenteral Nutrition in Children after Hematopoietic Stem Cell Transplantation

    Directory of Open Access Journals (Sweden)

    María Auxiliadora Baena-Gómez

    2015-08-01

    Full Text Available Background: Traditionally, lipids used in parenteral nutrition (PN are based on ω-6 fatty acid-rich vegetable oils, such as soybean oil, with potential adverse effects involving oxidative stress. Methods: We evaluated the antioxidant defense system in children, after hematopoietic stem cell transplantation (HSCT, who were randomized to use a lipid emulsion with fish oil or soybean oil. Blood samples at baseline, at 10 days, and at the end of the PN were taken to analyze plasma retinol, α-tocopherol, β-carotene, coenzyme Q9 and coenzyme Q10 levels, and catalase (CAT, glutathione reductase (GR, glutathione peroxidase (GPOX, and superoxide dismutase (SOD levels in lysed erythrocytes. Results: An increase in plasma α-tocopherol levels in the group of patients receiving the fish oil-containing emulsion (FO compared with the group receiving the soybean emulsion was observed at day 10 of PN. Concurrently, plasma α-tocopherol increased in the FO group and β-carotene decreased in both groups at day 10 compared with baseline levels, being more significant in the group receiving the FO emulsion. Conclusion: FO-containing emulsions in PN could improve the antioxidant profile by increasing levels of α-tocopherol in children after HSCT who are at higher risk of suffering oxidative stress and metabolic disorders.

  2. Influence of Different Doses of Levofloxacin on Antioxidant Defense Systems and Markers of Renal and Hepatic Dysfunctions in Rats

    Directory of Open Access Journals (Sweden)

    Ebenezer Tunde Olayinka

    2015-01-01

    Full Text Available Levofloxacin (LFX is a broad spectrum fluoroquinolone antibiotic used in the treatment of infections such as pneumonia, chronic bronchitis, and sinusitis. The present study assessed the likely toxic effect of LFX on hepatic and renal tissues in rats. Twenty male Wistar rats were randomly divided into four treatment groups: A: control, B: 5 mg/kg bw LFX (half therapeutic dose, C: 10 mg/kg bw LFX (therapeutic dose, and D: 20 mg/kg bw LFX (double therapeutic dose. After seven days of administration, result indicated significant (P<0.05 increase in plasma ALT, AST, and ALP activities in the treated groups compared to control. Also, there was a significant increase in plasma creatinine, urea, and total bilirubin in the treated groups relative to control. Plasma total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglycerides also increased significantly in the treated groups relative to control. Also, hepatic MDA level increased significantly in all the treated groups. However, hepatic SOD, catalase, and GST activities were significantly reduced in the LFX-treated animals. Moreover, GSH and ascorbic acid levels were significantly decreased in the LFX-treated groups relative to control. In conclusion, three doses of levofloxacin depleted antioxidant defense system and induced oxidative stress and hepatic and renal dysfunctions in rats.

  3. Aloin Protects Skin Fibroblasts from Heat Stress-Induced Oxidative Stress Damage by Regulating the Oxidative Defense System.

    Directory of Open Access Journals (Sweden)

    Fu-Wei Liu

    Full Text Available Oxidative stress is commonly involved in the pathogenesis of skin damage induced by environmental factors, such as heat stress. Skin fibroblasts are responsible for the connective tissue regeneration and the skin recovery from injury. Aloin, a bioactive compound in Aloe vera, has been reported to have various pharmacological activities, such as anti-inflammatory effects. The aim of this study was to investigate the protective effect of aloin against heat stress-mediated oxidative stress in human skin fibroblast Hs68 cells. Hs68 cells were first incubated at 43°C for 30 min to mimic heat stress. The study was further examined if aloin has any effect on heat stress-induced oxidative stress. We found that aloin protected Hs68 cells against heat stress-induced damage, as assessed by 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assay. Aloin protected Hs68 cells by regulating reactive oxygen species production and increasing the levels of glutathione, cytosolic and mitochondrial superoxide dismutase. Aloin also prevented the elevation of thiobarbituric acid reactive substances and the reduction of 8-OH-dG induced by heat stress. These results indicated that aloin protected human skin fibroblasts from heat stress-induced oxidative stress damage by regulating the oxidative defense system.

  4. Aloin Protects Skin Fibroblasts from Heat Stress-Induced Oxidative Stress Damage by Regulating the Oxidative Defense System.

    Science.gov (United States)

    Liu, Fu-Wei; Liu, Fu-Chao; Wang, Yu-Ren; Tsai, Hsin-I; Yu, Huang-Ping

    2015-01-01

    Oxidative stress is commonly involved in the pathogenesis of skin damage induced by environmental factors, such as heat stress. Skin fibroblasts are responsible for the connective tissue regeneration and the skin recovery from injury. Aloin, a bioactive compound in Aloe vera, has been reported to have various pharmacological activities, such as anti-inflammatory effects. The aim of this study was to investigate the protective effect of aloin against heat stress-mediated oxidative stress in human skin fibroblast Hs68 cells. Hs68 cells were first incubated at 43°C for 30 min to mimic heat stress. The study was further examined if aloin has any effect on heat stress-induced oxidative stress. We found that aloin protected Hs68 cells against heat stress-induced damage, as assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase assay. Aloin protected Hs68 cells by regulating reactive oxygen species production and increasing the levels of glutathione, cytosolic and mitochondrial superoxide dismutase. Aloin also prevented the elevation of thiobarbituric acid reactive substances and the reduction of 8-OH-dG induced by heat stress. These results indicated that aloin protected human skin fibroblasts from heat stress-induced oxidative stress damage by regulating the oxidative defense system. PMID:26637174

  5. CADMIUM AND NICKEL COMBINED EFFECT ON ASCORBATE-DEPENDENT MECHANISM OF ANTIOXIDATIVE DEFENSE SYSTEM OF PEA

    Directory of Open Access Journals (Sweden)

    Artiushenko Tetiana A.

    2014-04-01

    towards control level, i.e. there is a significant induction of the synthesis process. In addition, the amount of ascorbate oxidized forms, as in previous research period, continued to increase. After 24 hours of experiment we registered similar to 12 hours trend of changes in the content of ascorbic acid metabolites. Violation of pro-/antioxidant balance also accompanied by increased ascorbate peroxidase activity. In the pea root system there is the tendency to increase the ascorbate peroxidase activity and this is in concordance with the increasing content of ascorbic and dehydroascorbate acids. At the beginning of stressful activities caused by cadmium and nickel compounds in the leaves is not the case of ascorbate peroxidase activity changes, although 7-hour influence resulted in a statistically significant increase in activity specified oxidoreductase. By the action of heavy metals in low concentrations the content of ascorbic acid in pea leaves increased by 14%. High content in the medium of at least one of the metals since 7-hour exposure caused the depletion of antioxidant resources, as evidenced by the decrease of ascorbic acid in the assimilation organs. After 24 hours of experiment we observed a slowdown in ascorbate peroxidase activity, which could be explained by the lack of ascorbic acid under prolonged stress exposure.

  6. Real-time monitoring of cellular dynamics using a microfluidic cell culture system with integrated electrode array and potentiostat

    DEFF Research Database (Denmark)

    Zor, Kinga; Vergani, M.; Heiskanen, Arto;

    2011-01-01

    A versatile microfluidic, multichamber cell culture and analysis system with an integrated electrode array and potentiostat suitable for electrochemical detection and microscopic imaging is presented in this paper. The system, which allows on-line electrode cleaning and modification, was developed...... for real-time monitoring of cellular dynamics, exemplified in this work by monitoring of redox metabolism inside living yeast cells and dopamine release from PC12 cells....

  7. Mogućnosti implementacije savremenih sistema upravljanja saobraćajem u sistem odbrane / Possibilities of implementation of contemporary traffic management systems in the defense system

    Directory of Open Access Journals (Sweden)

    Srđan Ljubojević

    2007-10-01

    Full Text Available Savremeni trendovi u upravljanju saobraćajem okrenuti su ka efikasnijem iskorišćenju postojećih kapaciteta. Ovaj rad je izraz težnje da se sagleda uloga inteligentnih transportnih sistema u takvom pristupu upravljanju saobraćajem, kao i da se ukaže na budućnost sistema odbrane na tom polju. / The latest trends in traffic management focus on more efficient usage of existing capacities. This paper tries to perceive the role of Intelligent Transportation Systems within such an approach to traffic management as well as to point out to the future of the Defense System in this field.

  8. Micro 3D cell culture systems for cellular behavior studies: Culture matrices, devices, substrates, and in-situ sensing methods.

    Science.gov (United States)

    Choi, Jonghoon; Lee, Eun Kyu; Choo, Jaebum; Yuh, Junhan; Hong, Jong Wook

    2015-09-01

    Microfabricated systems equipped with 3D cell culture devices and in-situ cellular biosensing tools can be a powerful bionanotechnology platform to investigate a variety of biomedical applications. Various construction substrates such as plastics, glass, and paper are used for microstructures. When selecting a construction substrate, a key consideration is a porous microenvironment that allows for spheroid growth and mimics the extracellular matrix (ECM) of cell aggregates. Various bio-functionalized hydrogels are ideal candidates that mimic the natural ECM for 3D cell culture. When selecting an optimal and appropriate microfabrication method, both the intended use of the system and the characteristics and restrictions of the target cells should be carefully considered. For highly sensitive and near-cell surface detection of excreted cellular compounds, SERS-based microsystems capable of dual modal imaging have the potential to be powerful tools; however, the development of optical reporters and nanoprobes remains a key challenge. We expect that the microsystems capable of both 3D cell culture and cellular response monitoring would serve as excellent tools to provide fundamental cellular behavior information for various biomedical applications such as metastasis, wound healing, high throughput screening, tissue engineering, regenerative medicine, and drug discovery and development. PMID:26358782

  9. Studies of cellular radiosensitivity in hereditary disorders of nervous system and muscle

    Energy Technology Data Exchange (ETDEWEB)

    Brennan, S.; Lewis, P.D. (Royal Postgraduate Medical School, London (UK))

    1983-12-01

    Skin fibroblasts from patients with familial dysautonomia, Duchenne muscular dystrophy and Charcot-Marie-Tooth disease show normal sensitivity to ionising radiation, as measured by post-irradiation clonal growth. Previous reports of cellular hypersensitivity to ionising radiation and other DNA-damaging agents in familial dysautonomia and Duchenne muscular dystrophy have not been confirmed.

  10. Studies of cellular radiosensitivity in hereditary disorders of nervous system and muscle

    International Nuclear Information System (INIS)

    Skin fibroblasts from patients with familial dysautonomia, Duchenne muscular dystrophy and Charcot-Marie-Tooth disease show normal sensitivity to ionising radiation, as measured by post-irradiation clonal growth. Previous reports of cellular hypersensitivity to ionising radiation and other DNA-damaging agents in familial dysautonomia and Duchenne muscular dystrophy have not been confirmed. (author)

  11. Studies of cellular radiosensitivity in hereditary disorders of nervous system and muscle.

    OpenAIRE

    Brennan, S; Lewis, P.D.

    1983-01-01

    Skin fibroblasts from patients with familial dysautonomia, Duchenne muscular dystrophy and Charcot-Marie-Tooth disease show normal sensitivity to ionising radiation, as measured by post-irradiation clonal growth. Previous reports of cellular hypersensitivity to ionising radiation and other DNA-damaging agents in familial dysautonomia and Duchenne muscular dystrophy have not been confirmed.

  12. Heat-induced regulation of antioxidant defense system and nutrient accumulation in hexaploid bread wheat (triticum aestivum l.)

    International Nuclear Information System (INIS)

    Ten cultivars (five registered S-24, Inqlab-91, Saher-2006, Fsd-2008, and Lasani, and five candidate cultivars P.B-18, M.P-65, S.H-20, AARI-10, and G.A-20) of spring wheat (Triticum aestivum L.) were examined for high temperature stress tolerance. Plants were grown in soil filled pots in the Botanical Garden of the Department of Botany University of Agriculture Faisalabad, Pakistan. Three different temperature regimes (30, 40 and 50 degree C) were applied at two different growth stages (tillering and boot) for three temperature durations 30, 60 and 90 min in a growth chamber. The leaf and root samples were collected after two weeks of temperature treatment and then analyzed for enzymatic and non-enzymatic antioxidants as well as inorganic nutrients (N, P, K+, Ca2+). At the end, data obtained were statistically analyzed to distinguish heat tolerant from non-tolerant wheat cultivars. After appraisal of growth, antioxidant defense system and uptake of nutrients it was found that cvs. S-24, Inqlab-91, Saher-2006, Fsd-2008, Lasani and G.A-20 exhibited better thermo-tolerance capabilities than the other wheat cultivars (P.B-18, M.P-65, S.H-20, AARI-10). Among the thermo-tolerant wheat cultivars, G.A-20 and Lasani were superior in maintaining shoot fresh weights and shoot length, high antioxidant activities and better nutrient uptake at both tillering and boot stages. The response of all cultivars to heat stress applied at the tillering stage or boot stage was almost the same. (author)

  13. Variations in Growth, Photosynthesis and Defense System Among Four Weed Species Under Increased UV-B Radiation

    Institute of Scientific and Technical Information of China (English)

    Shiwen Wang; Liusheng Duan; Anthony Egrinya Eneji; Zhaohu Li

    2007-01-01

    Weed tolerance of UV-B radiation varies with species, and the radiation could affect weed ecology and management.Variations in growth, photosynthesis and defense system among four important agronomic weeds, Abutilon theophrasti Medik, Amaranthus retroflexus L., Digitaria sangulnalis (L.) Scop and Chloris virgata Swartz, under increased UV-B radiation (ambient and increased radiation at 2.7, 5.4 and 10.8 kJ·m-2·d-1) were studied in the greenhouse experiment. After 2 weeks of radiation, the shoots' dry mass decreased with increasing UV-B radiation except for D. sanguinalis. The reduction in biomass was the result of changes in morphology and physiology.Higher levels of UV-B treatment decreased the leaf area, plant height, net photosynthetic rate and chlorophyll contents, while it increased the contents of wax and UV-B absorbing compound in all species, except for A. retroflexus,which did not increase significantly. The activity of superoxide dismutase, catalase, ascorbate peroxide and the content of ascorbic acid changed differently among the weed species as UV-B radiation increased. D. sanguinalis was the most tolerant and A. retroflexus the most sensitive to increased UV-B radiation. The results also show that the two grass species (D. sanguinalis and C. virgata) were more tolerant to UV-B radiation than the two broadleafed species (A. theophrasti and A. retroflexus). The UV-B absorbing compound and leaf wax played important roles against UV-B damages in the two grass weeds. The overall results suggest that weed community, competition and management will be altered by continuous ozone depletion.

  14. Beta-carotene reduces oxidative stress, improves glutathione metabolism and modifies antioxidant defense systems in lead-exposed workers

    International Nuclear Information System (INIS)

    The aim of this study was to determine whether beta-carotene administration reduces oxidative stress and influences antioxidant, mainly glutathione-related, defense systems in workers chronically exposed to lead. The population consisted of two randomly divided groups of healthy male volunteers exposed to lead. Workers in the first group (reference group) were not administered any antioxidants, while workers in the second group (CAR group) were treated orally with 10 mg of beta-carotene once a day for 12 weeks. Biochemical analysis included measuring markers of lead-exposure and oxidative stress in addition to the levels and activities of selected antioxidants. After treatment, levels of malondialdehyde, lipid hydroperoxides and lipofuscin significantly decreased compared with the reference group. However, the level of glutathione significantly increased compared with the baseline. Treatment with beta-carotene also resulted in significantly decreased glutathione peroxidase activity compared with the reference group, while the activities of other glutathione-related enzymes and of superoxide dismutase were not significantly changed. However, the activities of glucose-6-phosphate dehydrogenase and catalase, as well as the level of alpha-tocopherol, were significantly higher after treatment compared with the baseline. Despite controversy over the antioxidant properties of beta-carotene in vivo, our findings showed reduced oxidative stress after beta-carotene supplementation in chronic lead poisoning. - Highlights: • Beta-carotene reduces oxidative stress in lead-exposed workers. • Beta-carotene elevates glutathione level in lead-exposed workers. • Beta-carotene administration could be beneficial in lead poisoning

  15. Role of 9-lipoxygenase and α-dioxygenase oxylipin pathways as modulators of local and systemic defense.

    Science.gov (United States)

    Vicente, Jorge; Cascón, Tomás; Vicedo, Begonya; García-Agustín, Pilar; Hamberg, Mats; Castresana, Carmen

    2012-07-01

    Plant 9-lipoxygenases (9-LOX) and α-dioxygenases (α-DOX) initiate the synthesis of oxylipins after bacterial infection. Here, the role of these enzymes in plants' defense was investigated using individual Arabidopsis thaliana lox1 and dox1 mutants and a double lox1 dox1 mutant. Studies with Pseudomonas syringae pv. tomato (Pst) revealed the enhanced susceptibility of lox1 to the virulent strain Pst DC3000 and the partial impairment of lox1 and dox1 mutants to activate systemic acquired resistance. Notably, both defects were enhanced in the lox1 dox1 plants as compared with individual mutants. We found that pre-treatment with 9-LOX- and α-DOX-generated oxylipins protected plant tissues against bacterial infection. The strongest effect in this respect was exerted by 9-ketooctadecatrienoic acid (9-KOT), which is produced from linolenic acid by 9-LOX. Quantification of 9-KOT revealed its accumulation after bacterial infection. The levels were reduced in lox1 and lox1 dox1 plants but strongly increased in the dox1 mutant due to metabolic interaction of the two pathways. Transcriptional analyses indicated that 9-KOT pre-treatment modifies hormone homeostasis during bacterial infection. The nature of the changes detected suggested that 9-KOT interferes with the hormonal changes caused by bacterial effectors. This notion was substantiated by the finding that 9-KOT failed to reduce the growth of PstDC3000hrpA, a mutant compromised in effector secretion, and of the avirulent strain Pst DC3000 avrRpm1. Further support for the action of the 9-LOX- and α-DOX-oxylipin pathways as modulators of hormone homeostasis was the observation that lox1 dox1 seedlings are hypersensitive to the growth-inhibitory effect of ABA and showed enhanced activation of ABA-inducible marker genes as compared with wild-type plants. PMID:22199234

  16. Role of 9-Lipoxygenase and α-Dioxygenase Oxylipin Pathways as Modulators of Local and Systemic Defense

    Institute of Scientific and Technical Information of China (English)

    Jorge Vicente; Tomás Cascón; Begonya Vicedo; Pilar García-Agustín; Mats Hamberg; Carmen Castresana

    2012-01-01

    Plant 9-lipoxygenases(9-LOX)and α-dioxygenases(α-DOX)initiate the synthesis of oxylipins after bacterial infection.Here,the role of these enzymes in plants' defense was investigated using individual Arabidopsis thaliana lox1 and dox1 mutants and a double lox1 dox1 mutant.Studies with Pseudomonas syringae pv.tomato(Pst)revealed the enhanced susceptibility of lox1 to the virulent strain Pst DC3000 and the partial impairment of lox1 and dox1 mutants to activate systemic acquired resistance.Notably,both defects were enhanced in the lox1 dox1 plants as compared with individual mutants.We found that pre-treatment with 9-LOX- and α-DOX-generated oxylipins protected plant tissues against bacterial infection.The strongest effect in this respect was exerted by 9-ketooctadecatrienoic acid(9-KOT),which is produced from linolenic acid by 9-LOX.Quantification of 9-KOT revealed its accumulation after bacterial infection.The levels were reduced in lox1 and lox1 dox1 plants but strongly increased in the dox1 mutant due to metabolic interaction of the two pathways.Transcriptional analyses indicated that 9-KOT pre-treatment modifies hormone homeostasis during bacterial infection.The nature of the changes detected suggested that 9-KOT interferes with the hormonal changes caused by bacterial effectors.This notion was substantiated by the finding that 9-KOT failed to reduce the growth of PstDC3000hrpA,a mutant compromised in effector secretion,and of the avirulent strain Pst DC3000 avrRpm1.Further support for the action of the 9-LOX- and α-DOX-oxylipin pathways as modulators of hormone homeostasis was the observation that lox1 dox1 seedlings are hypersensitive to the growth-inhibitory effect of ABA and showed enhanced activation of ABA-inducible marker genes as compared with wild-type plants.

  17. Beta-carotene reduces oxidative stress, improves glutathione metabolism and modifies antioxidant defense systems in lead-exposed workers

    Energy Technology Data Exchange (ETDEWEB)

    Kasperczyk, Sławomir, E-mail: kaslav@mp.pl [Dept. of Biochemistry, School of Medicine with the Division of Dentistry, Medical University of Silesia, ul. Jordana 19, 41-808 Zabrze (Poland); Dobrakowski, Michał [Dept. of Biochemistry, School of Medicine with the Division of Dentistry, Medical University of Silesia, ul. Jordana 19, 41-808 Zabrze (Poland); Kasperczyk, Janusz [Dept. of Environmental Medicine and Epidemiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, ul. Jordana 19, 41-808 Zabrze (Poland); Ostałowska, Alina; Zalejska-Fiolka, Jolanta; Birkner, Ewa [Dept. of Biochemistry, School of Medicine with the Division of Dentistry, Medical University of Silesia, ul. Jordana 19, 41-808 Zabrze (Poland)

    2014-10-01

    The aim of this study was to determine whether beta-carotene administration reduces oxidative stress and influences antioxidant, mainly glutathione-related, defense systems in workers chronically exposed to lead. The population consisted of two randomly divided groups of healthy male volunteers exposed to lead. Workers in the first group (reference group) were not administered any antioxidants, while workers in the second group (CAR group) were treated orally with 10 mg of beta-carotene once a day for 12 weeks. Biochemical analysis included measuring markers of lead-exposure and oxidative stress in addition to the levels and activities of selected antioxidants. After treatment, levels of malondialdehyde, lipid hydroperoxides and lipofuscin significantly decreased compared with the reference group. However, the level of glutathione significantly increased compared with the baseline. Treatment with beta-carotene also resulted in significantly decreased glutathione peroxidase activity compared with the reference group, while the activities of other glutathione-related enzymes and of superoxide dismutase were not significantly changed. However, the activities of glucose-6-phosphate dehydrogenase and catalase, as well as the level of alpha-tocopherol, were significantly higher after treatment compared with the baseline. Despite controversy over the antioxidant properties of beta-carotene in vivo, our findings showed reduced oxidative stress after beta-carotene supplementation in chronic lead poisoning. - Highlights: • Beta-carotene reduces oxidative stress in lead-exposed workers. • Beta-carotene elevates glutathione level in lead-exposed workers. • Beta-carotene administration could be beneficial in lead poisoning.

  18. Role of garlic oil and selenium as stimulators to some antioxidant defense system in irradiated male rats

    International Nuclear Information System (INIS)

    The increased level of lipid peroxide product; malondialdehyde (MDA) in various tissues of irradiated animals, may play a crucial role in damaging effects of exposure to ionizing radiation mediated by reactive free radicals generation. The protective efficiency of oral administration of garlic oil (500 mg/kg b.wt) three times weekly together with a single intraperitoneal injection of selenium (0.5 mg/kg b.wt) weekly for two weeks was examined on some antioxidant defense system as well as ultrastructural study in rats subjected to fractionated doses of gamma radiation up to a dose level of 6 Gy (1.5 Gy day after day). Exposure to gamma radiation induced a significant elevation in the level of malondialdehyde in plasma and liver and non-significant change was observed in superoxide dismutase activity (SOD). Also a significant increase was occurred in hepatic glutathione (GSH) content and glutathione peroxidase (GSHPx) activity. The pretreatment of irradiated rats with garlic oil and selenium caused a significant decrease in elevated level of MDA and a significant increase in the activity of SOD, GSHPx and GSH contents in both blood and liver. Concerning the ultrastructure studies, liver of irradiated rats showed abnormal shape of nucleus and nucleus membrane, swollen mitochondria with ruptured cristae, rupture of endoplasmic reticulum and vaculated cytoplasm, while intestine of irradiated rats exhibited deformed, shortened and abnormal structure of microvilli, accumulation of nuclear chromatin and dilatation of terminal web layer. In group of rats treated with garlic oil and selenium before exposure to gamma radiation, noticeable amelioration in ultrastructure changes of liver and intestine induced by irradiation was observed indicating a beneficial radioprotective role of both garlic oil and selenium

  19. Onset of herbivore-induced resistance in systemic tissue primed for jasmonate-dependent defenses is activated by abscisic acid

    Directory of Open Access Journals (Sweden)

    Irene A. Vos

    2013-12-01

    Full Text Available In Arabidopsis, the MYC2 transcription factor on the one hand and the AP2/ERF transcription factors ORA59 and ERF1 on the other hand regulate distinct branches of the jasmonic acid (JA signaling pathway in an antagonistic fashion, co-regulated by abscisic acid (ABA and ethylene, respectively. Feeding by larvae of the specialist herbivorous insect Pieris rapae (small cabbage white butterfly results in activation of the MYC-branch and concomitant suppression of the ERF-branch in insect-damaged leaves. Here we investigated differential JA signaling activation in undamaged systemic leaves of P. rapae-infested plants. We found that the MYC2 transcription factor gene was induced both in the local insect-damaged leaves and the systemic undamaged leaves of P. rapae-infested Arabidopsis plants. However, in contrast to the insect-damaged leaves, the undamaged tissue did not show activation of the MYC-branch marker gene VSP1. Comparison of the hormone signal signature revealed that the levels of JA and (+-7-iso-jasmonoyl-L-isoleucine (JA-Ile raised to similar extents in locally damaged and systemically undamaged leaves, but the production of ABA and the JA precursor 12-oxo-phytodienoic acid (OPDA was enhanced only in the local herbivore-damaged leaves, and not in the distal undamaged leaves. Challenge of undamaged leaves of pre-infested plants with either P. rapae larvae or exogenously applied ABA led to potentiated expression levels of MYC2 and VSP1, with the latter reaching extremely high expression levels. Moreover, P. rapae-induced resistance, as measured by reduction of caterpillar growth on pre-infested plants, was blocked in the ABA biosynthesis mutant aba2-1, that was also impaired in P. rapae-induced expression of VSP1. Together, these results suggest that ABA is a crucial regulator of herbivore-induced resistance by activating primed JA-regulated defense responses upon secondary herbivore attack in Arabidopsis.

  20. Playing Defense

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    With the U.S. financial system still reeling and recession looming on Europe’s doorstep, the world is looking to China as a calm port in the storm. But China has its own set of woes as clouds gather over its economy as a result of the domino effect from the overseas economic downturn and lingering inflationary fears. On October 26, Zhou Xiaochuan, Governor of the People’s Bank of China, delivered a report on the country’s current economic situation and the safeguards that the government has put in place to fend off economic risks. Excerpts follow.

  1. Combination of Polymer Technology and Carbon Nanotube Array for the Development of an Effective Drug Delivery System at Cellular Level

    Directory of Open Access Journals (Sweden)

    Riggio Cristina

    2009-01-01

    Full Text Available Abstract In this article, a carbon nanotube (CNT array-based system combined with a polymer thin film is proposed as an effective drug release device directly at cellular level. The polymeric film embedded in the CNT array is described and characterized in terms of release kinetics, while in vitro assays on PC12 cell line have been performed in order to assess the efficiency and functionality of the entrapped agent (neural growth factor, NGF. PC12 cell differentiation, following incubation on the CNT array embedding the alginate delivery film, demonstrated the effectiveness of the proposed solution. The achieved results indicate that polymeric technology could be efficiently embedded in CNT array acting as drug delivery system at cellular level. The implication of this study opens several perspectives in particular in the field of neurointerfaces, combining several functions into a single platform.

  2. Improving Spectral Capacity and Wireless Network Coverage by Cognitive Radio Technology and Relay Nodes in Cellular Systems

    DEFF Research Database (Denmark)

    Frederiksen, Flemming Bjerge

    2008-01-01

    Methods to enhance the use of the frequency spectrum by automatical spectrum sensing plus spectrum sharing in a cognitive radio technology context have been presented and discussed in this paper. Ideas to improve the wireless transmission by orthogonal OFDM-based communication and to increase the...... coverage of cellular systems by future wireless networks, relay channels, relay stations and collaborate radio have been presented as well. A revised hierarchical deployment of the future wireless and wired networks are shortly discussed....

  3. Interleukin 17-Mediated Host Defense against Candida albicans

    Directory of Open Access Journals (Sweden)

    Florian Sparber

    2015-08-01

    Full Text Available Candida albicans is part of the normal microbiota in most healthy individuals. However, it can cause opportunistic infections if host defenses are breached, with symptoms ranging from superficial lesions to severe systemic disease. The study of rare congenital defects in patients with chronic mucocutaneous candidiasis led to the identification of interleukin-17 (IL-17 as a key factor in host defense against mucosal fungal infection. Experimental infections in mice confirmed the critical role of IL-17 in mucocutaneous immunity against C. albicans. Research on mouse models has also contributed importantly to our current understanding of the regulation of IL-17 production by different cellular sources and its effector functions in distinct tissues. In this review, we highlight recent findings on IL-17-mediated immunity against C. albicans in mouse and man.

  4. The Capsid Protein of Turnip Crinkle Virus Overcomes two Separate Defense Barriers to Facilitate Viral Systemic Movement in Arabidopsis

    Science.gov (United States)

    The capsid protein (CP) of Turnip crinkle virus (TCV) is a multi-functional protein needed for virus assembly, suppression of RNA silencing-based antiviral defense, and long distance movement in infected plants. In this report, we have examined genetic requirements for the different functions of TCV...

  5. 47 CFR 22.909 - Cellular markets.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 2 2010-10-01 2010-10-01 false Cellular markets. 22.909 Section 22.909... Cellular Radiotelephone Service § 22.909 Cellular markets. Cellular markets are standard geographic areas used by the FCC for administrative convenience in the licensing of cellular systems. Cellular...

  6. Modulation of exogenous glutathione in antioxidant defense system against Cd stress in the two barley genotypes differing in Cd tolerance.

    Science.gov (United States)

    Chen, Fei; Wang, Fang; Wu, Feibo; Mao, Weihua; Zhang, Guoping; Zhou, Meixue

    2010-08-01

    Soil cadmium (Cd) contamination has posed a serious problem for safe food production and become a potential agricultural and environmental hazard worldwide. Greenhouse hydroponic experiments were conducted to investigate the modulation of exogenous GSH (reduced glutathione) in antioxidant defense system against the Cd-induced toxicity in plants exposed to 5 muM Cd using two barley genotypes differing in Cd tolerance. Addition of 20 mg L(-1) GSH in 5 muM Cd culture medium significantly alleviated Cd-induced growth inhibition, especially for the sensitive genotype Dong 17 and dramatically depressed O(2)(-), H(2)O(2) and malondialdehyde (MDA) accumulation. GSH mediated intracellular GSH content to keep the level over the control especially in the case of Cd-induced GSH reduction. External GSH counteracted Cd-induced alterations of certain antioxidant enzymes, e.g. brought root dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and glutathione peroxidase (GPX) activities of the both genotypes down towards the control level, but elevated the depressed ascorbate peroxidase (APX) and catalase (CAT) activities in Dong 17 after 10-15 d treatment. The examination of APX and superoxide dismutase (SOD) isoenzymes revealed GSH significantly increased MnSOD, sAPX and tAPX activities in the both genotypes, and strongly stimulated Cd-induced decrease in cAPX in the sensitive genotype. Furthermore, External GSH up-regulated root cAPX and leaf cAPX, CAT1, and CAT2 expression at transcript level in Dong 17 to achieve stimulation. These data, especially from the results of depressed O(2)(-), H(2)O(2) and MDA accumulation and elevated Cd-induced decrease in GSH content and APX (strongly stimulated cAPX, sAPX and tAPX) and CAT activities by GSH addition in the sensitive genotype, suggest that elevated intracellular GSH and stimulated APX (especially cAPX, sAPX and tAPX iosenzymes) and CAT activities, when concerning ROS scavenging systems, play an important role

  7. Transcriptional Characteristics of Xa21-mediated Defense Responses in Rice

    Institute of Scientific and Technical Information of China (English)

    Qiang Gan; Hui Bai; Xianfeng Zhao; Yong Tao; Haipan Zeng; Yuning Han; Wenyuan Song; Lihuang Zhu; Guozhen Liu

    2011-01-01

    Bacterial blight, caused by Xanthomonas oryzae pv. oryzae (Xoo), is the most destructive bacterial disease of rice. The cloned rice gene Xa21 confers resistance to a broad spectrum of Xoo races. To identify genes involved in Xa21-mediated immunity, a whole-genome oligonucleotide microarray of rice was used to profile the expression of rice genes between incompatible interactions and mock treatments at 0, 4, 8, 24, 72 and 120 h post inoculation (hpi) or between incompatible and compatible interactions at 4 hpi, respectively. A total of 441 differentially expressed genes, designated as XDGs (Xa21 mediated differentially expressed genes), were identified. Based on their functional annotations, the XDGs were assigned to 14 categories, including defense-related, signaling, transcriptional regulators. Most of the defense-related genes belonged to the pathogenesis-related gene family, which was induced dramatically at 72 and 120 hpi. Interestingly, most signaling and transcriptional regulator genes were downregulated at 4 and 8 hpi, suggesting that negative regulation of cellular signaling may play a role in the Xa21-mediated defense response. Comparison of expression profiles between Xa21- and other R gene-mediated defense systems revealed interesting common responses. Representative XDGs with supporting evidences were also discussed.

  8. Cellular Automata in Modular Space : Rigid Systems – Volume I – Number III

    OpenAIRE

    Fridenfalk, Mikael

    2015-01-01

    This book series presents a new type of cellular automata for 2D pattern generation, characterized by a high reproduction rate, in combination with the application of a small-sized 2D modular square lattice. The presented patterns are in the spirit of mathematical minimalism, generated from rudimentary kernels and a minimal set of rules. In similarity with fractals, this new concept could provide for the generation of patterns and geometries with applications in areas such as, visual arts, lo...

  9. Cellular Automata in Modular Space : Rigid Systems – Volume V – Number I

    OpenAIRE

    Fridenfalk, Mikael

    2015-01-01

    This book series presents a new type of cellular automata for 2D pattern generation, characterized by a high reproduction rate, in combination with the application of a small-sized 2D modular square lattice. The presented patterns are in the spirit of mathematical minimalism, generated from rudimentary kernels and a minimal set of rules. In similarity with fractals, this new concept could provide for the generation of patterns and geometries with applications in areas such as, visual arts, lo...

  10. Cellular Automata in Modular Space : Rigid Systems – Volume III – Number IV

    OpenAIRE

    Fridenfalk, Mikael

    2015-01-01

    This book series presents a new type of cellular automata for 2D pattern generation, characterized by a high reproduction rate, in combination with the application of a small-sized 2D modular square lattice. The presented patterns are in the spirit of mathematical minimalism, generated from rudimentary kernels and a minimal set of rules. In similarity with fractals, this new concept could provide for the generation of patterns and geometries with applications in areas such as, visual arts, lo...

  11. Cellular Automata in Modular Space : Rigid Systems – Volume I – Number II

    OpenAIRE

    Fridenfalk, Mikael

    2015-01-01

    This book series presents a new type of cellular automata for 2D pattern generation, characterized by a high reproduction rate, in combination with the application of a small-sized 2D modular square lattice. The presented patterns are in the spirit of mathematical minimalism, generated from rudimentary kernels and a minimal set of rules. In similarity with fractals, this new concept could provide for the generation of patterns and geometries with applications in areas such as, visual arts, lo...

  12. Cellular Automata in Modular Space : Rigid Systems – Volume IV – Number IV

    OpenAIRE

    Fridenfalk, Mikael

    2015-01-01

    This book series presents a new type of cellular automata for 2D pattern generation, characterized by a high reproduction rate, in combination with the application of a small-sized 2D modular square lattice. The presented patterns are in the spirit of mathematical minimalism, generated from rudimentary kernels and a minimal set of rules. In similarity with fractals, this new concept could provide for the generation of patterns and geometries with applications in areas such as, visual arts, lo...

  13. Cellular Automata in Modular Space : Rigid Systems – Volume III – Number II

    OpenAIRE

    Fridenfalk, Mikael

    2015-01-01

    This book series presents a new type of cellular automata for 2D pattern generation, characterized by a high reproduction rate, in combination with the application of a small-sized 2D modular square lattice. The presented patterns are in the spirit of mathematical minimalism, generated from rudimentary kernels and a minimal set of rules. In similarity with fractals, this new concept could provide for the generation of patterns and geometries with applications in areas such as, visual arts, lo...

  14. Cellular Automata in Modular Space : Rigid Systems – Volume II – Number V

    OpenAIRE

    Fridenfalk, Mikael

    2015-01-01

    This book series presents a new type of cellular automata for 2D pattern generation, characterized by a high reproduction rate, in combination with the application of a small-sized 2D modular square lattice. The presented patterns are in the spirit of mathematical minimalism, generated from rudimentary kernels and a minimal set of rules. In similarity with fractals, this new concept could provide for the generation of patterns and geometries with applications in areas such as, visual arts, lo...

  15. Cellular Automata in Modular Space : Rigid Systems – Volume IV – Number III

    OpenAIRE

    Fridenfalk, Mikael

    2015-01-01

    This book series presents a new type of cellular automata for 2D pattern generation, characterized by a high reproduction rate, in combination with the application of a small-sized 2D modular square lattice. The presented patterns are in the spirit of mathematical minimalism, generated from rudimentary kernels and a minimal set of rules. In similarity with fractals, this new concept could provide for the generation of patterns and geometries with applications in areas such as, visual arts, lo...

  16. Cellular Automata in Modular Space : Rigid Systems – Volume I – Number V

    OpenAIRE

    Fridenfalk, Mikael

    2015-01-01

    This book series presents a new type of cellular automata for 2D pattern generation, characterized by a high reproduction rate, in combination with the application of a small-sized 2D modular square lattice. The presented patterns are in the spirit of mathematical minimalism, generated from rudimentary kernels and a minimal set of rules. In similarity with fractals, this new concept could provide for the generation of patterns and geometries with applications in areas such as, visual arts, lo...

  17. Cellular Automata in Modular Space : Rigid Systems – Volume IV – Number I

    OpenAIRE

    Fridenfalk, Mikael

    2015-01-01

    This book series presents a new type of cellular automata for 2D pattern generation, characterized by a high reproduction rate, in combination with the application of a small-sized 2D modular square lattice. The presented patterns are in the spirit of mathematical minimalism, generated from rudimentary kernels and a minimal set of rules. In similarity with fractals, this new concept could provide for the generation of patterns and geometries with applications in areas such as, visual arts, lo...

  18. Microfluidics-Based in Vivo Mimetic Systems for the Study of Cellular Biology

    OpenAIRE

    Kim, Donghyuk; Wu, Xiaojie; Young, Ashlyn T.; Haynes, Christy L.

    2014-01-01

    Conspectus The human body is a complex network of molecules, organelles, cells, tissues, and organs: an uncountable number of interactions and transformations interconnect all the system’s components. In addition to these biochemical components, biophysical components, such as pressure, flow, and morphology, and the location of all of these interactions play an important role in the human body. Technical difficulties have frequently limited researchers from observing cellular biology as it oc...

  19. Phenomenon of Psychological Defense

    Directory of Open Access Journals (Sweden)

    Elena T. Sokolova

    2011-01-01

    Full Text Available The author discusses the controversial issues of formation and functioning of psy¬chological defense mechanisms in ontogenesis and in personality disorders as they are represented in classical and contemporary psychoanalysis, in cognitivism and communication theory. The paper emphasizes the role of cognitive organi¬zation (style, sign-symbolic mediation, representative system of object relations and attachments in individual typological variability of the level organization of ciency of personal and social adaptation, in maturity and mental health of personality

  20. Abnormalities in the cellular phase of blood fibrinolytic activity in systemic lupus erythematosus and in venous thromboembolism

    International Nuclear Information System (INIS)

    Fibrinolytic activities of whole blood and plasma were determined by 125I-fibrin radiometric assay in 16 normal subjects, and in 11 patients with systemic lupus erythematosus (SLE), 14 with progressive systemic sclerosis (PSS), 23 with venous thromboembolic disease, and 20 patients awaiting elective surgery. Mean whole blood and plasma activities for patients with PSS, and for those awaiting elective surgery, were similar to normal values, as was the mean plasma activity in patients with SLE. However, mean whole blood activity in SLE was significantly decreased compared with normals (p less than 0.05), with mean plasma activity accounting for 44% of mean whole blood activity (compared with 17% in normal subjects), representing a 67% decrease in mean calculated cellular phase activity in SLE, when compared with normals. Since the numbers of cells (neutrophils, monocytes) possibly involved in cellular activity were not decreased, the findings suggest a functional defect in fibrinolytic activity of one or more blood cell types in SLE. An additional finding was the participation of the cellular phase as well as the well-known plasma phase of blood in the fibrinolytic response to thromboembolism

  1. Construction of the Disciplinary System of Defense S & T Information Science & Its Core Field Research%装备科技情报学科体系构筑与核心领域研究

    Institute of Scientific and Technical Information of China (English)

    符福峘

    2003-01-01

    Five issues regarding the construction of the disciplinary system of defense S & T information science are discussed, i. e. the concept and the research subject; the basic factors; the research object and major fields; the na-ture and academic position; and the function and mission. It's pointed out that strengthening the research on the theoreti-cal system of defense S & T information science and using this system to guide information practice and promote the devel-opment of defense S & T information undertakings are of great significance.

  2. Understanding Defense Mechanisms.

    Science.gov (United States)

    Cramer, Phebe

    2015-12-01

    Understanding defense mechanisms is an important part of psychotherapy. In this article, we trace the history of the concept of defense, from its origin with Freud to current views. The issue of defense as an unconscious mechanism is examined. The question of whether defenses are pathological, as well as their relation to pathology, is discussed. The effect of psychotherapy on the use of defenses, and their relation to a therapeutic alliance is explored. A series of empirical research studies that demonstrate the functioning of defense mechanisms and that support the theory is presented. Research also shows that as part of normal development, different defenses emerge at different developmental periods, and that gender differences in defense use occur. PMID:26583439

  3. A novel cell traction force microscopy to study multi-cellular system.

    Directory of Open Access Journals (Sweden)

    Xin Tang

    2014-06-01

    Full Text Available Traction forces exerted by adherent cells on their microenvironment can mediate many critical cellular functions. Accurate quantification of these forces is essential for mechanistic understanding of mechanotransduction. However, most existing methods of quantifying cellular forces are limited to single cells in isolation, whereas most physiological processes are inherently multi-cellular in nature where cell-cell and cell-microenvironment interactions determine the emergent properties of cell clusters. In the present study, a robust finite-element-method-based cell traction force microscopy technique is developed to estimate the traction forces produced by multiple isolated cells as well as cell clusters on soft substrates. The method accounts for the finite thickness of the substrate. Hence, cell cluster size can be larger than substrate thickness. The method allows computing the traction field from the substrate displacements within the cells' and clusters' boundaries. The displacement data outside these boundaries are not necessary. The utility of the method is demonstrated by computing the traction generated by multiple monkey kidney fibroblasts (MKF and human colon cancerous (HCT-8 cells in close proximity, as well as by large clusters. It is found that cells act as individual contractile groups within clusters for generating traction. There may be multiple of such groups in the cluster, or the entire cluster may behave a single group. Individual cells do not form dipoles, but serve as a conduit of force (transmission lines over long distances in the cluster. The cell-cell force can be either tensile or compressive depending on the cell-microenvironment interactions.

  4. Cellular Biology in Terms of Stochastic Nonlinear Biochemical Dynamics: Emergent Properties, Isogenetic Variations and Chemical System Inheritability

    Science.gov (United States)

    Qian, Hong

    2010-12-01

    Based on a stochastic, nonlinear, open biochemical reaction system perspective, we present an analytical theory for cellular biochemical processes. The chemical master equation (CME) approach provides a unifying mathematical framework for cellular modeling. We apply this theory to both self-regulating gene networks and phosphorylation-dephosphorylation signaling modules with feedbacks. Two types of bistability are illustrated in mesoscopic biochemical systems: one that has a macroscopic, deterministic counterpart and another that does not. In certain cases, the latter stochastic bistability is shown to be a "ghost" of the extinction phenomenon. We argue the thermal fluctuations inherent in molecular processes do not disappear in mesoscopic cell-sized nonlinear systems; rather they manifest themselves as isogenetic variations on a different time scale. Isogenetic biochemical variations in terms of the stochastic attractors can have extremely long lifetime. Transitions among discrete stochastic attractors spend most of the time in "waiting", exhibit punctuated equilibria. It can be naturally passed to "daughter cells" via a simple growth and division process. The CME system follows a set of nonequilibrium thermodynamic laws that include non-increasing free energy F( t) with external energy drive Q hk ≥0, and total entropy production rate e p =- dF/ dt+ Q hk ≥0. In the thermodynamic limit, with a system's size being infinitely large, the nonlinear bistability in the CME exhibits many of the characteristics of macroscopic equilibrium phase transition.

  5. Virtual cellular manufacturing system based on resource element approach and analyzing its performance over different basic layouts

    Directory of Open Access Journals (Sweden)

    G. R. Esmaeilian

    2012-01-01

    Full Text Available This research aims to present how choosing a suitable layout can improve the performance of virtual cellular manufacturing systems (VCMSs, especially minimizing the material flow between machines required by each family group. To present the efficacy of basic layouts on performances of VCMSs, a multi-objective mathematical model with a goal programming (GP approach is developed to generate VCMSs based on resource-elements (REs. The formulated model is coded in Lingo software and is run over functional and distributed arrangements of the same machines. The performance and the validity of the developed model are checked by a numerical example taken from the literature. The objective function of the mathematical model is measured for that example over two mentioned layout to compare the performance of the generated systems. Moreover, because of the material handling costs importance, material flows are measured to find the best option as a basic layout for VCMSs. To compare the performance of the generated system with the classical cellular manufacturing system (CMS, cell capacity utilization (CCU is employed as an independent criterion to evaluate each system. The result illustrates the priority of distributed layouts for generating RE-based VCMSs because of its flexibility, minimizing the objective function for the mathematical model, and smaller material flow by the components. In addition, the generated VCMSs outperforms the classical CMS from the CCU point of view.

  6. BER Analysis to Increase Capacity of A Cellular System Using SDMA Over a Multipath Rayleigh Fading Channel

    Directory of Open Access Journals (Sweden)

    M. Gangadharappa

    2010-07-01

    Full Text Available In this paper, we have presented how the capacity of cellular system can be enhanced by using Space Division Multiple Access (SDMA with directive antenna in mobile communication systems. One of the biggest draw back of wireless environment is the limited bandwidth. However,the users sharing this limited bandwidth have been increased considerably. SDMA is a technology by which the capacity of the existing mobile communication system can economically be increased. Adaptive Antenna provides a spot beam for each users in the cells as it moves. This paper analysis the comparison of average Bit Error Rate (BER of SDMA and Code Division Multiple Access (CDMAtechnique and the way in which SDMA can be introduced to increase the capacity of a cellular system in Frequency Selective Multipath Rayleigh Fading Channel. The BER is found for a standard omni directional base station antenna, and the another set of curve is found for flat top beam having a directivity of 8 dB. We see that SDMA offers improved capacity in wireless communication system.

  7. A Versatile System for the In-Field Non-Destructive Characterization of Radioactive Waste Packages and of Objects in the Defense against Nuclear Threats

    International Nuclear Information System (INIS)

    In-filed non-destructive characterization of radioactive waste packages and of objects in the defense of nuclear threats requires purpose-built but similar equipment. Based on commercially available measuring devices like dose-rate and gamma detectors, X-ray and gamma-transmission sources etc. a versatile and mobile mechanical positioning system for these devices is designed, assembled and operated facilitating basic to even complex measuring procedures. Several in-field measuring campaigns resulted in its further optimization. Today an highly mobile and flexible mechanical manipulator system is available supporting nearly all types of required measuring devices thus rising to nearly all occasions. (authors)

  8. Research of HLA Air-Defense Simulation System Based on Agent%基于Agent的HLA空防对抗仿真系统研究

    Institute of Scientific and Technical Information of China (English)

    李云芳; 万晓冬

    2012-01-01

    以防空作战为背景,介绍了防空作战仿真系统的组成结构,对防空作战中各成员的功能进行了分析,建立了系统中火力单元指控的Agent模型结构.通过分析各Agent之间的联系,建立了多Agent之间的交互关系,并详细描述了火力单元指控的射击指挥行为过程.最后,给出了基于Agent模型的火力单元指挥控制仿真实例,验证模型的可行性.%The structure of air-defense combat simulation system was introduced, and the member function of air-defense combat was analyzed for air defense operations. Then, Agent model structure of firepower unit command and control was built Through the analysis of the relationship between the various Agent, the interaction between the Agent was established. Also, detailed descriptions of firing command action plan was given. Finally, The simulation example of firepower unit command and control is given based on Agent model, verified the feasibility of the model.

  9. Linear programming embedded particle swarm optimization for solving an extended model of dynamic virtual cellular manufacturing systems

    Directory of Open Access Journals (Sweden)

    H. Rezazadeh

    2009-04-01

    Full Text Available The concept of virtual cellular manufacturing system (VCMS is finding acceptance among researchers as an extension to grouptechnology. In fact, in order to realize benefits of cellular manufacturing system in the functional layout, the VCMS createsprovisional groups of resources (machines, parts and workers in the production planning and control system. This paperdevelops a mathematical model to design the VCMS under a dynamic environment with a more integrated approach whereproduction planning, system reconfiguration and workforce requirements decisions are incorporated. The advantages of theproposed model are as follows: considering the operations sequence, alternative process plans for part types, machine timecapacity,worker time‐capacity, cross‐training, lot splitting, maximal cell size, balanced workload for cells and workers. Anefficient linear programming embedded particle swarm optimization algorithm is used to solve the proposed model. Thealgorithm searches over the 0‐1 integer variables and for each 0‐1 integer solution visited; corresponding values of integervariables are determined by solving a linear programming sub‐problem using the simplex algorithm. Numerical examples showthat the proposed method is efficient and effective in searching for near optimal solutions.

  10. Cellular automata-based artificial life system of horizontal gene transfer

    Directory of Open Access Journals (Sweden)

    Ji-xin Liu

    2016-02-01

    Full Text Available Mutation and natural selection is the core of Darwin's idea about evolution. Many algorithms and models are based on this idea. However, in the evolution of prokaryotes, more and more researches have indicated that horizontal gene transfer (HGT would be much more important and universal than the authors had imagined. Owing to this mechanism, the prokaryotes not only become adaptable in nearly any environment on Earth, but also form a global genetic bank and a super communication network with all the genes of the prokaryotic world. Under this background, they present a novel cellular automata model general gene transfer to simulate and study the vertical gene transfer and HGT in the prokaryotes. At the same time, they use Schrodinger's life theory to formulate some evaluation indices and to discuss the intelligence and cognition of prokaryotes which is derived from HGT.

  11. Prevalence and Mechanisms of Dynamic Chemical Defenses in Tropical Sponges

    OpenAIRE

    Rohde, Sven; Nietzer, Samuel; Schupp, Peter J

    2015-01-01

    Sponges and other sessile invertebrates are lacking behavioural escape or defense mechanisms and rely therefore on morphological or chemical defenses. Studies from terrestrial systems and marine algae demonstrated facultative defenses like induction and activation to be common, suggesting that sessile marine organisms also evolved mechanisms to increase the efficiency of their chemical defense. However, inducible defenses in sponges have not been investigated so far and studies on activated d...

  12. Dynamics of uptake and metabolism of small molecules in cellular response systems.

    Directory of Open Access Journals (Sweden)

    Maria Werner

    Full Text Available BACKGROUND: Proper cellular function requires uptake of small molecules from the environment. In response to changes in extracellular conditions cells alter the import and utilization of small molecules. For a wide variety of small molecules the cellular response is regulated by a network motif that combines two feedback loops, one which regulates the transport and the other which regulates the subsequent metabolism. RESULTS: We analyze the dynamic behavior of two widespread but logically distinct two-loop motifs. These motifs differ in the logic of the feedback loop regulating the uptake of the small molecule. Our aim is to examine the qualitative features of the dynamics of these two classes of feedback motifs. We find that the negative feedback to transport is accompanied by overshoot in the intracellular amount of small molecules, whereas a positive feedback to transport removes overshoot by boosting the final steady state level. On the other hand, the negative feedback allows for a rapid initial response, whereas the positive feedback is slower. We also illustrate how the dynamical deficiencies of one feedback motif can be mitigated by an additional loop, while maintaining the original steady-state properties. CONCLUSIONS: Our analysis emphasizes the core of the regulation found in many motifs at the interface between the metabolic network and the environment of the cell. By simplifying the regulation into uptake and the first metabolic step, we provide a basis for elaborate studies of more realistic network structures. Particularly, this theoretical analysis predicts that FeS cluster formation plays an important role in the dynamics of iron homeostasis.

  13. Cellular and molecular mechanisms of HGF/Met in the cardiovascular system.

    Science.gov (United States)

    Gallo, Simona; Sala, Valentina; Gatti, Stefano; Crepaldi, Tiziana

    2015-12-01

    Met tyrosine kinase receptor, also known as c-Met, is the HGF (hepatocyte growth factor) receptor. The HGF/Met pathway has a prominent role in cardiovascular remodelling after tissue injury. The present review provides a synopsis of the cellular and molecular mechanisms underlying the effects of HGF/Met in the heart and blood vessels. In vivo, HGF/Met function is particularly important for the protection of the heart in response to both acute and chronic insults, including ischaemic injury and doxorubicin-induced cardiotoxicity. Accordingly, conditional deletion of Met in cardiomyocytes results in impaired organ defence against oxidative stress. After ischaemic injury, activation of Met provides strong anti-apoptotic stimuli for cardiomyocytes through PI3K (phosphoinositide 3-kinase)/Akt and MAPK (mitogen-activated protein kinase) cascades. Recently, we found that HGF/Met is also important for autophagy regulation in cardiomyocytes via the mTOR (mammalian target of rapamycin) pathway. HGF/Met induces proliferation and migration of endothelial cells through Rac1 (Ras-related C3 botulinum toxin substrate 1) activation. In fibroblasts, HGF/Met antagonizes the actions of TGFβ1 (transforming growth factor β1) and AngII (angiotensin II), thus preventing fibrosis. Moreover, HGF/Met influences the inflammatory response of macrophages and the immune response of dendritic cells, indicating its protective function against atherosclerotic and autoimmune diseases. The HGF/Met axis also plays an important role in regulating self-renewal and myocardial regeneration through the enhancement of cardiac progenitor cells. HGF/Met has beneficial effects against myocardial infarction and endothelial dysfunction: the cellular and molecular mechanisms underlying repair function in the heart and blood vessels are common and include pro-angiogenic, anti-inflammatory and anti-fibrotic actions. Thus administration of HGF or HGF mimetics may represent a promising therapeutic agent for the

  14. Wi-Fi Hotspot Auto-Discovery: A Practical & Energy-Aware System for Smart Objects using Cellular Signals

    OpenAIRE

    Nithyananthan Poosamani; Injong Rhee

    2015-01-01

    The Internet of Things (IoT) paradigm aims to interconnect a variety of heterogeneous Smart Objects (SO) using energy-efficient methodologies and standard communication protocols. A majority of consumer devices sold today come equipped with wireless LAN and cellular technology to connect with the world-wide network. To discover Wi-Fi hot spots, there is a need for constant scanning of Wi-Fi radio in these devices and results in significant battery drain. We present PRiSM, a practical system t...

  15. A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons.

    Science.gov (United States)

    Williams, Michael R; Fricano-Kugler, Catherine J; Getz, Stephanie A; Skelton, Patrick D; Lee, Jeonghoon; Rizzuto, Christian P; Geller, Joseph S; Li, Meijie; Luikart, Bryan W

    2016-01-01

    Retroviruses expressing a fluorescent protein, Cas9, and a small guide RNA are used to mimic nonsense PTEN mutations from autism patients in developing mouse neurons. We compare the cellular phenotype elicited by CRISPR-Cas9 to those elicited using shRNA or Cre/Lox technologies and find that knockdown or knockout (KO) produced a corresponding moderate or severe neuronal hypertrophy in all cells. In contrast, the Cas9 approach produced missense and nonsense Pten mutations, resulting in a mix of KO-equivalent hypertrophic and wild type-like phenotypes. Importantly, despite this mixed phenotype, the neuronal hypertrophy resulting from Pten loss was evident on average in the population of manipulated cells. Having reproduced the known Pten KO phenotype using the CRISPR-Cas9 system we design viruses to target a gene that has recently been associated with autism, KATNAL2. Katnal2 deletion in the mouse results in decreased dendritic arborization of developing neurons. We conclude that retroviral implementation of the CRISPR-Cas9 system is an efficient system for cellular phenotype discovery in wild-type animals. PMID:27161796

  16. A Retroviral CRISPR-Cas9 System for Cellular Autism-Associated Phenotype Discovery in Developing Neurons

    Science.gov (United States)

    Williams, Michael R.; Fricano-Kugler, Catherine J.; Getz, Stephanie A.; Skelton, Patrick D.; Lee, Jeonghoon; Rizzuto, Christian P.; Geller, Joseph S.; Li, Meijie; Luikart, Bryan W.

    2016-01-01

    Retroviruses expressing a fluorescent protein, Cas9, and a small guide RNA are used to mimic nonsense PTEN mutations from autism patients in developing mouse neurons. We compare the cellular phenotype elicited by CRISPR-Cas9 to those elicited using shRNA or Cre/Lox technologies and find that knockdown or knockout (KO) produced a corresponding moderate or severe neuronal hypertrophy in all cells. In contrast, the Cas9 approach produced missense and nonsense Pten mutations, resulting in a mix of KO-equivalent hypertrophic and wild type-like phenotypes. Importantly, despite this mixed phenotype, the neuronal hypertrophy resulting from Pten loss was evident on average in the population of manipulated cells. Having reproduced the known Pten KO phenotype using the CRISPR-Cas9 system we design viruses to target a gene that has recently been associated with autism, KATNAL2. Katnal2 deletion in the mouse results in decreased dendritic arborization of developing neurons. We conclude that retroviral implementation of the CRISPR-Cas9 system is an efficient system for cellular phenotype discovery in wild-type animals. PMID:27161796

  17. Automatic design system for a steel sheet pile cellular cofferdam and its evaluation; Koya ita cell shiki kozobutsu no kihon sekkei no jidoka oyobi system no hyoka

    Energy Technology Data Exchange (ETDEWEB)

    Sekine, Y.; Ito, A.; Yokota, H. [Port and Harbour Research Inst., Kanagawa (Japan)

    1995-03-01

    A new design scheme has recently been introduced to a sheet pile cellular cofferdam, which takes the effect of the embedded part of a sheet pile on the structural stability into account. On the basis of the modification of the design way, the automatic design system for this type of structure has been established. Port engineers are able to conduct basic design of a breakwater and a seaward made of the sheet pile cellular cofferdam. Parametric studies have been conducted using this automatic design system. It has been confirmed based on the study that this system provides the reasonable structural sections, embedded length, and so on. Some particular characteristics of this type of structures have also been provided in this present report. 5 refs., 59 figs., 4 tabs.

  18. Estimation of the PM2.5 Pollution Levels in Beijing Based on Nighttime Light Data from the Defense Meteorological Satellite Program-Operational Linescan System

    OpenAIRE

    Runya Li; Xiangnan Liu; Xuqing Li

    2015-01-01

    Nighttime light data record the artificial light on the Earth’s surface and can be used to estimate the degree of pollution associated with particulate matter with an aerodynamic diameter of less than 2.5 μm (PM2.5) in the ground-level atmosphere. This study proposes a simple method for monitoring PM2.5 concentrations at night by using nighttime light imagery from the Defense Meteorological Satellite Program-Operational Linescan System (DMSP-OLS). This research synthesizes remote sensing and...

  19. Design and testing of a new microcalorimetric vessel for use with living cellular systems and in titration experiments.

    Science.gov (United States)

    Nordmark, M G; Laynez, J; Schön, A; Suurkuusk, J; Wadsö, I

    1984-12-01

    A new microcalorimetric vessel primarily intended for use with living cellular systems and in titration experiments has been designed and tested. The vessel, which forms a modular system, fits into an ampoule measuring cylinder of the LKB 'BioActivity Monitor'. It can be used with different sample cups, volume 1-3 ml, and can be equipped with different types of stirrer and sample holders for cellular materials. Experiments can be performed with or without medium perfusing through the vessel. Small quantities of reagents can be added to the sample compartment during the measurements. Stepwise calorimetric titrations can be performed by an automatic procedure. Test experiments reported include results of measurements with human T-lymphoma cells in stirred suspension and melanoma cells adhered to a polystyrene film in a stirred perfusing medium. Results from titration experiments where N-acetyl-D-alanine was bound to ristocetin A are reported, delta G degree' = -16.5 +/- 0.2 kJ mol-1 and delta H degree' = -32.1 +/- 0.4 kJ mol-1. PMID:6397499

  20. U.S. Department of Energy Space and Defense Power Systems Program Ten-Year Strategic Plan, Volume 1 and Volume 2

    Energy Technology Data Exchange (ETDEWEB)

    Dwight, Carla

    2013-06-01

    The Department of Energy's Space and Defense Power Systems program provides a unique capability for supplying power systems that function in remote or hostile environments. This capability has been functioning since the early 1960s and counts the National Aeronautics and Space Administration as one of its most prominent customers. This enabling technology has assisted the exploration of our solar system including the planets Jupiter, Saturn, Mars, Neptune, and soon Pluto. This capability is one-of-kind in the world in terms of its experience (over five decades), breadth of power systems flown (over two dozen to date) and range of power levels (watts to hundreds of watts). This document describes the various components of that infrastructure, work scope, funding needs, and its strategic plans going forward.

  1. Calcium Mitigates Arsenic Toxicity in Rice Seedlings by Reducing Arsenic Uptake and Modulating the Antioxidant Defense and Glyoxalase Systems and Stress Markers

    Directory of Open Access Journals (Sweden)

    Anisur Rahman

    2015-01-01

    Full Text Available The effect of exogenous calcium (Ca on hydroponically grown rice seedlings was studied under arsenic (As stress by investigating the antioxidant and glyoxalase systems. Fourteen-day-old rice (Oryza sativa L. cv. BRRI dhan29 seedlings were exposed to 0.5 and 1 mM Na2HAsO4 alone and in combination with 10 mM CaCl2 (Ca for 5 days. Both levels of As caused growth inhibition, chlorosis, reduced leaf RWC, and increased As accumulation in the rice seedlings. Both doses of As in growth medium induced oxidative stress through overproduction of reactive oxygen species (ROS by disrupting the antioxidant defense and glyoxalase systems. Exogenous application of Ca along with both levels of As significantly decreased As accumulation and restored plant growth and water loss. Calcium supplementation in the As-exposed rice seedlings reduced ROS production, increased ascorbate (AsA content, and increased the activities of monodehydroascorbate reductase (MDHAR, dehydroascorbate reductase (DHAR, catalase (CAT, glutathione peroxidase (GPX, superoxide dismutase (SOD, and the glyoxalase I (Gly I and glyoxalase II (Gly II enzymes compared with seedlings exposed to As only. These results suggest that Ca supplementation improves rice seedlings tolerance to As-induced oxidative stress by reducing As uptake, enhancing their antioxidant defense and glyoxalase systems, and also improving growth and physiological condition.

  2. Prokaryotic homologs of Argonaute proteins are predicted to function as key components of a novel system of defense against mobile genetic elements

    Directory of Open Access Journals (Sweden)

    van der Oost John

    2009-08-01

    Full Text Available Abstract Background In eukaryotes, RNA interference (RNAi is a major mechanism of defense against viruses and transposable elements as well of regulating translation of endogenous mRNAs. The RNAi systems recognize the target RNA molecules via small guide RNAs that are completely or partially complementary to a region of the target. Key components of the RNAi systems are proteins of the Argonaute-PIWI family some of which function as slicers, the nucleases that cleave the target RNA that is base-paired to a guide RNA. Numerous prokaryotes possess the CRISPR-associated system (CASS of defense against phages and plasmids that is, in part, mechanistically analogous but not homologous to eukaryotic RNAi systems. Many prokaryotes also encode homologs of Argonaute-PIWI proteins but their functions remain unknown. Results We present a detailed analysis of Argonaute-PIWI protein sequences and the genomic neighborhoods of the respective genes in prokaryotes. Whereas eukaryotic Ago/PIWI proteins always contain PAZ (oligonucleotide binding and PIWI (active or inactivated nuclease domains, the prokaryotic Argonaute homologs (pAgos fall into two major groups in which the PAZ domain is either present or absent. The monophyly of each group is supported by a phylogenetic analysis of the conserved PIWI-domains. Almost all pAgos that lack a PAZ domain appear to be inactivated, and the respective genes are associated with a variety of predicted nucleases in putative operons. An additional, uncharacterized domain that is fused to various nucleases appears to be a unique signature of operons encoding the short (lacking PAZ pAgo form. By contrast, almost all PAZ-domain containing pAgos are predicted to be active nucleases. Some proteins of this group (e.g., that from Aquifex aeolicus have been experimentally shown to possess nuclease activity, and are not typically associated with genes for other (putative nucleases. Given these observations, the apparent extensive

  3. Cellular responses induced by environmental stress factors in Arctic Seabird chicks : Responses of the antioxidant defense system and autophagic lysosomal processes related to contaminant exposure and food restriction

    OpenAIRE

    Hegseth, Marit Nøst

    2011-01-01

    The papers of this thesis are not available in Munin: 1. Marit Nøst Hegseth, Lionel Camus, Lisa Bjørnsdatter Helgason, Raffaella Bocchetti, Geir Wing Gabrielsen and Francesco Regoli: 'Hepatic antioxidant responses related to levels of PCBs and metals in chicks of three Arctic seabird species', Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology (2011) 154: 28-35. Available at http://dx.doi.org/10.1016/j.cbpc.2011.02.008 2. Marit Nøst Hegseth, Lionel Camus, Stefania Go...

  4. New Concepts and Theories For Intelligent Control of Cellular Manufacturing Systems

    DEFF Research Database (Denmark)

    Langer, Gilad

    1996-01-01

    evolve around the idea of integrating intelligent elements into the control systems of the manufacturing systems. The research will concentrate on integration of manufacturing units by use of intelligent control mechanisms, information technology and the material handling as the key integrators.......This paper will present some new theories such as biological manufacturing system, the fractal factory theory, holonic manufacturing systems, and multi-agent theory in the context of manufacturing systems. The objective of the paper is to show how these theories can contribute to develop the new...... concepts that are required for controlling the manufacturing systems of the future. Thereafter it will present the potential for improvement of the manufacturing system by introduction and implementation of these theories. The paper tries to encapsulate the main area of my Ph.D. thesis research which will...

  5. On Modeling Large-Scale Multi-Agent Systems with Parallel, Sequential and Genuinely Asynchronous Cellular Automata

    International Nuclear Information System (INIS)

    We study certain types of Cellular Automata (CA) viewed as an abstraction of large-scale Multi-Agent Systems (MAS). We argue that the classical CA model needs to be modified in several important respects, in order to become a relevant and sufficiently general model for the large-scale MAS, and so that thus generalized model can capture many important MAS properties at the level of agent ensembles and their long-term collective behavior patterns. We specifically focus on the issue of inter-agent communication in CA, and propose sequential cellular automata (SCA) as the first step, and genuinely Asynchronous Cellular Automata (ACA) as the ultimate deterministic CA-based abstract models for large-scale MAS made of simple reactive agents. We first formulate deterministic and nondeterministic versions of sequential CA, and then summarize some interesting configuration space properties (i.e., possible behaviors) of a restricted class of sequential CA. In particular, we compare and contrast those properties of sequential CA with the corresponding properties of the classical (that is, parallel and perfectly synchronous) CA with the same restricted class of update rules. We analytically demonstrate failure of the studied sequential CA models to simulate all possible behaviors of perfectly synchronous parallel CA, even for a very restricted class of non-linear totalistic node update rules. The lesson learned is that the interleaving semantics of concurrency, when applied to sequential CA, is not refined enough to adequately capture the perfect synchrony of parallel CA updates. Last but not least, we outline what would be an appropriate CA-like abstraction for large-scale distributed computing insofar as the inter-agent communication model is concerned, and in that context we propose genuinely asynchronous CA. (author)

  6. Takeover Defenses and Competition

    OpenAIRE

    Martijn Cremers; Vinay Nair; Urs Peyer

    2007-01-01

    This paper studies the interaction between takeover defenses and product market competition. We find that firms in more competitive industries have more takeover defenses. This is the opposite result from what one would expect if takeover defenses always constitute an inefficient outcome that increases agency costs and managerial entrenchment. A novel explantion is provided by considering the nature of the relationship between the firm and the product (or labor) market. For firms in industrie...

  7. Norwegian cyber defense

    OpenAIRE

    Stensboel, Karl Birger

    2013-01-01

    Approved for public release; distribution is unlimited. This thesis postulates the need for a more proactive approach to cyber defense in Norway and offers recommendations about how Norway can be better prepared to counter cyber threats. It finds that Norways strategic infrastructure is vulnerable to cyber attacks and that Norway has no coherent strategy for meeting this challenge. The thesis argues that an effective cyber defense requires a wide range of offensive and defensive measures a...

  8. Temporal order of evolution of DNA replication systems inferred by comparison of cellular and viral DNA polymerases

    Directory of Open Access Journals (Sweden)

    Koonin Eugene V

    2006-12-01

    Full Text Available Abstract Background The core enzymes of the DNA replication systems show striking diversity among cellular life forms and more so among viruses. In particular, and counter-intuitively, given the central role of DNA in all cells and the mechanistic uniformity of replication, the core enzymes of the replication systems of bacteria and archaea (as well as eukaryotes are unrelated or extremely distantly related. Viruses and plasmids, in addition, possess at least two unique DNA replication systems, namely, the protein-primed and rolling circle modalities of replication. This unexpected diversity makes the origin and evolution of DNA replication systems a particularly challenging and intriguing problem in evolutionary biology. Results I propose a specific succession for the emergence of different DNA replication systems, drawing argument from the differences in their representation among viruses and other selfish replicating elements. In a striking pattern, the DNA replication systems of viruses infecting bacteria and eukaryotes are dominated by the archaeal-type B-family DNA polymerase (PolB whereas the bacterial replicative DNA polymerase (PolC is present only in a handful of bacteriophage genomes. There is no apparent mechanistic impediment to the involvement of the bacterial-type replication machinery in viral DNA replication. Therefore, I hypothesize that the observed, markedly unequal distribution of the replicative DNA polymerases among the known cellular and viral replication systems has a historical explanation. I propose that, among the two types of DNA replication machineries that are found in extant life forms, the archaeal-type, PolB-based system evolved first and had already given rise to a variety of diverse viruses and other selfish elements before the advent of the bacterial, PolC-based machinery. Conceivably, at that stage of evolution, the niches for DNA-viral reproduction have been already filled with viruses replicating with the

  9. Role of Antagonistic Microorganisms and Organic Amendment in Stimulating the Defense System of Okra Against Root Rotting Fungi.

    Science.gov (United States)

    Shafique, Hafiza Asma; Sultana, Viqar; Ara, Jehan; Ehteshamul-Haque, Syed; Athar, Mohammad

    2015-01-01

    Without application of chemical pesticides control of soilborne diseases is a great challenge. Stimulation of natural plant's defense is considered as one of the most promising alternative strategy for crop protection. Organic amendment of soil besides direct suppressing the pathogen, has been reported to have an influence on phytochemicals in plants. In the present study, Pseudomonas aeruginosa, a plant growth promoting rhizobacterium and Paecilomyces lilacinus, an egg parasite of root knot and cysts nematodes were examined individually and in combination in soil amended with cotton cake for suppressing the root rotting fungi and stimulating the synthesis of polyphenols and improving the antioxidant status in okra. Application of P. aeruginosa and P. lilacinus in soil amended with cotton cake significantly (P Rhizoctonia solani. Combine use of biocontrol agents in cotton cake amended soil showed maximum positive impact on plant growth, polyphenol concentration and antioxidant activity in okra. PMID:26373176

  10. Crushing behavior of hybrid hexagonal/octagonal cellular composite system: Aramid/carbon hybrid composite

    International Nuclear Information System (INIS)

    Highlights: • Replacing the carbon/epoxy by aramid/epoxy did not affect the peak load. • The post crush force efficiency showed improvements by using aramid/epoxy packing. • The specific energy showed improvement by using aramid/epoxy packing. • Brittle fracture dominated the crush behavior for the carbon/epoxy packing. • Plastic deformation without fracture was observed for aramid/epoxy packing. - Abstract: In the current paper a series of experiments were conducted to assess the crashworthiness of cellular hexagonal/octagonal composite device. Each device composed of 6 cells of carbon fiber reinforced composite (CFRP). Different arrangements of the octagonal and the hexagonal cells were studied. All the configurations were filled with foam. The main objective of the current paper was to examine the effect of using the aramid/epoxy instead of the carbon/epoxy layers to pack the device. The specimens were tested under quasi-static compression loading up to complete crushing. The results showed that the packing material did not have a significant effect for the case of all hexagonal open cells. For the other configurations, introducing the aramid/epoxy instead of the carbon/epoxy showed improvements in the stroke efficiency, the crush load stability, the average crushing load, the energy absorbed and the specific energy absorption. In order to understand the mechanisms that led to this improvement, the packing material were examine after crushing using an optical microscope and a scanning electron microscope (SEM). For the carbon/epoxy, the images showed many failure mechanisms whereas, for the aramid/epoxy, only delamination was noted

  11. Self-defense System in Germany and Its Significance to China%德国正当防卫制度及其对我国的启示

    Institute of Scientific and Technical Information of China (English)

    龙正凤

    2015-01-01

    德国的正当防卫制度是建立在个人权利的保护和法保护两大基本原则之上的。德国正当防卫制度所保护的法益、对紧急防卫的限度、对紧急帮助权、对防卫过当等有关法律规定与我国的正当防卫制度不同。结合我国的立法实际,通过对德国正当防卫制度的立法审视,对于完善我国的正当防卫制度,具有重要的理论和实践意义。%German self-defense system is based on the two fundamental principles of protecting laws and individual rights, Compared with self-defense system in Germany,self-defense system in China is quite differences in terms of the protected legal interests,the limit of emergent defense,emergent help right,excessive defense and other relevant laws and regulations, On the basis of the reality of China legislation,it will have important theoretical and practical significance for improving self-defense sys-tem in China.

  12. 基于Multi-Agent的防空导弹武器系统模型设计%Design of Multi-Agent Based Model on Air Defense Missile Weapon System

    Institute of Scientific and Technical Information of China (English)

    陈宝印; 栾立秋; 张成斌

    2011-01-01

    建立科学的结构模型是进行防空导弹武器系统仿真的基础和关键环节.针对防空导弹武器系统的特点,提出了基于Multi-Agent的防空导弹武器系统模型建模方法,设计了防空导弹武器系统模型结构.利用Multi-Agent建模技术,把防空导弹武器系统实体映射成相应的Multi-Agent系统,并以Agent的形式对防空导弹武器系统这一客观复杂系统进行了深刻的认识,为防空导弹武器系统建模仿真奠定了基础.%Forming a scientific system model is the key of Air Defense Missile Weapon System simulation. According to the characters of Air Defense Missile Weapon System, a modeling method of multi-agent based model on Air Defense Missile Weapon System is given. The framework on Air Defense Missile Weapon System is designed. Using the Multi-Agent modeling technology, maps the Air Defense Missile Weapon System entity the corresponding Multi-Agent system, and carries on the profound understanding by the Agent form to Air Defense Missile Weapon System this objective complicated, which will be helpful to realize the modeling simulation on Air Defense Missile Weapon System.

  13. Cellular Automata based Encrypted ECG-hash Code Generation: An Application in Inter-human Biometric Authentication System

    Directory of Open Access Journals (Sweden)

    Subrata Nandi

    2014-10-01

    Full Text Available In this modern era, biometrics incorporate various mechanisms to recognize inimitable features of human beings by utilizing their biological and evident features. This paper proposes a novel technique for constructing a resilient and secure biometric recognition system. In this paper, an ECG-hash code of two distinct individuals has been formed by taking dot product of electrocardiogram (ECG feature matrices of two persons located at two different sites at respective databases. The validity of the system increases as samples from both persons, between whom the transmission takes place, are essential. Besides, electrocardiogram is such a unique feature of an individual that could not be compromised at any circumstance as contradictory to other features like fingerprints, face recognition etc. Moreover, the ECG-hash code is encrypted using rule vector of cellular automata that gives better security in terms of randomness of generated cipher text.

  14. Policy implications of the Strategic Defense Initiative

    International Nuclear Information System (INIS)

    Specific topics include: the technological feasibility of proposed components and architectures; the compatibility of the proposed systems with existing and proposed arms control agreements, with special emphasis upon the ABM Treaty, Outer Space Treaty, the Defense and Space Treaty, and the START Treaty; the compatibility of proposed systems with classical warfare doctrine and the four modern strategic nuclear doctrines of Massive Retaliation, Assured Destruction, Countervailing and Flexible Response; the economics of strategic defense including an assessment of overall governmental spending, of the suballocation for defense, and of the feasibility of defensive systems which are cost-effective at the margin; and, in summary, an assessment of the New Strategic Concept which balances arms control, offensive forces, and defensive forces. This study falls within the realm of defense policy analysis in that it attempts to determine whether the administration's proposed Strategic Defense Initiative, as well as the long-term strategic defensive systems derived from SDI research, constitute efficient, desirable allocation of scarce government resources - especially in a period of seemingly relaxed superpower tensions and numerous demands upon those resources

  15. Cellular Automata

    OpenAIRE

    Bagnoli, Franco

    1998-01-01

    An introduction to cellular automata (both deterministic and probabilistic) with examples. Definition of deterministic automata, dynamical properties, damage spreading and Lyapunov exponents; probabilistic automata and Markov processes, nonequilibrium phase transitions, directed percolation, diffusion; simulation techniques, mean field. Investigation themes: life, epidemics, forest fires, percolation, modeling of ecosystems and speciation. They represent my notes for the school "Dynamical Mod...

  16. INTERRELATION OF THE PHYSICAL IMAGE OF "I" AND THE PSYCHOLOGICAL DEFENSE MECHANISM OF DISABLED PERSON WITH THE DEFEAT OF THE MUSCULOSKELETAL SYSTEM

    Directory of Open Access Journals (Sweden)

    A. V. GOLTSOV

    2016-01-01

    Full Text Available The article contains the results of an empirical study based on identifying characteristics of the relationship of psychological defense mechanisms of personality and physical image "I" disabled with damage to the musculoskeletal system. Disability is associated with lesions of the musculoskeletal system and was due to a variety of injuries, according to their psychological effect, is a serious and difficult going through a crisis of identity. The author substantiates the relevance of the study, determines the degree of its scientific elaboration, formulates a hypothesis and tests it in the course of empirical research. The study found that self-pity, self-centeredness determines the attractiveness of anatomical exaggeration and social characteristics of the physical image of "I", as well as resentment, vulnerability, ambition, jealousy, hostility. The desire to overcome the traumatic circumstances, to correct their shortcomings determines an increase in the distortion of the image of the functional characteristics of the physical "I". Rationalization is the mechanism of psychological defense, determines the adequacy of their perception of physicality and desire for self-management of their physical "I".

  17. Silverleaf whitefly induces salicylic acid defenses and suppresses effectual jasmonic acid defenses.

    Science.gov (United States)

    Zarate, Sonia I; Kempema, Louisa A; Walling, Linda L

    2007-02-01

    The basal defenses important in curtailing the development of the phloem-feeding silverleaf whitefly (Bemisia tabaci type B; SLWF) on Arabidopsis (Arabidopsis thaliana) were investigated. Sentinel defense gene RNAs were monitored in SLWF-infested and control plants. Salicylic acid (SA)-responsive gene transcripts accumulated locally (PR1, BGL2, PR5, SID2, EDS5, PAD4) and systemically (PR1, BGL2, PR5) during SLWF nymph feeding. In contrast, jasmonic acid (JA)- and ethylene-dependent RNAs (PDF1.2, VSP1, HEL, THI2.1, FAD3, ERS1, ERF1) were repressed or not modulated in SLWF-infested leaves. To test for a role of SA and JA pathways in basal defense, SLWF development on mutant and transgenic lines that constitutively activate or impair defense pathways was determined. By monitoring the percentage of SLWF nymphs in each instar, we show that mutants that activate SA defenses (cim10) or impair JA defenses (coi1) accelerated SLWF nymphal development. Reciprocally, mutants that activate JA defenses (cev1) or impair SA defenses (npr1, NahG) slowed SLWF nymphal development. Furthermore, when npr1 plants, which do not activate downstream SA defenses, were treated with methyl jasmonate, a dramatic delay in nymph development was observed. Collectively, these results showed that SLWF-repressed, JA-regulated defenses were associated with basal defense to the SLWF. PMID:17189328

  18. Silverleaf Whitefly Induces Salicylic Acid Defenses and Suppresses Effectual Jasmonic Acid Defenses1[W][OA

    Science.gov (United States)

    Zarate, Sonia I.; Kempema, Louisa A.; Walling, Linda L.

    2007-01-01

    The basal defenses important in curtailing the development of the phloem-feeding silverleaf whitefly (Bemisia tabaci type B; SLWF) on Arabidopsis (Arabidopsis thaliana) were investigated. Sentinel defense gene RNAs were monitored in SLWF-infested and control plants. Salicylic acid (SA)-responsive gene transcripts accumulated locally (PR1, BGL2, PR5, SID2, EDS5, PAD4) and systemically (PR1, BGL2, PR5) during SLWF nymph feeding. In contrast, jasmonic acid (JA)- and ethylene-dependent RNAs (PDF1.2, VSP1, HEL, THI2.1, FAD3, ERS1, ERF1) were repressed or not modulated in SLWF-infested leaves. To test for a role of SA and JA pathways in basal defense, SLWF development on mutant and transgenic lines that constitutively activate or impair defense pathways was determined. By monitoring the percentage of SLWF nymphs in each instar, we show that mutants that activate SA defenses (cim10) or impair JA defenses (coi1) accelerated SLWF nymphal development. Reciprocally, mutants that activate JA defenses (cev1) or impair SA defenses (npr1, NahG) slowed SLWF nymphal development. Furthermore, when npr1 plants, which do not activate downstream SA defenses, were treated with methyl jasmonate, a dramatic delay in nymph development was observed. Collectively, these results showed that SLWF-repressed, JA-regulated defenses were associated with basal defense to the SLWF. PMID:17189328

  19. Managing complexity of cellular systems: theoretical tools for dynamic modeling of metabolic reaction networks

    NARCIS (Netherlands)

    Nikerel, I.E.

    2009-01-01

    The study on biological systems attracts more and more interest since these systems offer interesting solutions to problems in diverse areas ranging from sustainable production of chemicals and fuels, environmental problems to medical questions. This thesis is concerned with the effort of improvin

  20. New Concepts and Theories For Intelligent Control of Cellular Manufacturing Systems

    DEFF Research Database (Denmark)

    Langer, Gilad

    1996-01-01

    This paper will present some new theories such as biological manufacturing system, the fractal factory theory, holonic manufacturing systems, agile manufacturing, object orientation, multi-agent theory, artificial intelligence, and artificial life in the context of manufacturing systems....... The paper tries to encapsulate the main area of my Ph.D. thesis research which will evolve around the idea of integrating intelligent elements into the control systems of the manufacturing systems. Furthermore it intends to show how the curriculum and discussions of the IPS Ph.D. course will and have...... contributed to my research. The research will concentrate on integration of manufacturing units by use of intelligent control mechanisms, information technology and the material handling as the key integrators....

  1. Analysis of environment, safety, and health (ES{ampersand}H) management systems for Department of Energy (DOE) Defense Programs (DP) facilities

    Energy Technology Data Exchange (ETDEWEB)

    Neglia, A. V., LLNL

    1998-03-01

    The purpose of this paper is to provide a summary analysis and comparison of various environment, safety, and health (ES&H) management systems required of, or suggested for use by, the Departrnent of Energy Defense Programs` sites. The summary analysis is provided by means of a comparison matrix, a set of Vean diagrams that highlights the focus of the systems, and an `End Gate` filter diagram that integrates the three Vean diagrams. It is intended that this paper will act as a starting point for implementing a particular system or in establishing a comprehensive site-wide integrated ES&H management system. Obviously, the source documents for each system would need to be reviewed to assure proper implementation of a particular system. The matrix compares nine ES&H management systems against a list of elements generated by identifying the unique elements of all the systems. To simplify the matrix, the elements are listed by means of a brief title. An explanation of the matrix elements is provided in Attachment 2 entitled, `Description of System Elements.` The elements are categorized under the Total Quality Management (TQM) `Plan, Do, Check, Act` framework with the added category of `Policy`. (The TQM concept is explained in the `DOE Quality Management implementation Guidelines,` July 1997 (DOE/QM- 0008)). The matrix provides a series of columns and rows to compare the unique elements found in each of the management systems. A `V` is marked if the element is explicitly identified as part of the particular ES&H management system. An `X` is marked if the element is not found in the particular ES&H management system, or if it is considered to be inadequately addressed. A `?` is marked if incorporation of the element is not clear. Attachment I provides additional background information which explains the justification for the marks in the matrix cells. Through the Vean diagrams and the `End Gate` filter in Section 3, the paper attempts to pictorially display the focus of

  2. Developing land use scenario dynamics model by the integration of system dynamics model and cellular automata model

    Institute of Scientific and Technical Information of China (English)

    HE; Chunyang; SHI; Peijun; CHEN; Jin; Li; Xiaobing; PAN; Ya

    2005-01-01

    Modeling land use scenario changes and its potential impacts on the structure and function of the ecosystem in the typical regions are helpful to understanding the interactive mechanism between land use system and ecological system. A Land Use Scenario Dynamics (LUSD) model by the integration of System Dynamics (SD) model and Cellular Automata (CA) model is developed with land use scenario changes in northern China in the next 20 years simulated in this paper. The basic idea of LUSD model is to simulate the land use scenario demands by using SD model at first, then allocate the land use scenario patterns at the local scale with the considerations of land use suitability, inheritance ability and neighborhood effect by using CA model to satisfy the balance between land use scenario demands and supply. The application of LUSD model in northern China suggests that the model has the ability to reflect the complex behavior of land use system at different scales to some extent and is a useful tool for assessing the potential impacts of land use system on ecological system. In addition, the simulated results also indicate that obvious land use changes will take place in the farming-pastoral zone of northern China in the next 20 years with cultivated land and urban land being the most active land use types.

  3. RF and ELF Exposures from Cellular Phone Handsets: TDMA and CDMA Systems (invited paper)

    International Nuclear Information System (INIS)

    The future digital systems for personal communications have now been partially standardised, so it is possible to get a good idea of the exposure power levels and time variation of the power, which may be assumed to be the two biologically relevant parameters. Using GSM and the coming UMTS system the relevant details are discussed, where the main difference is between the highly periodic TDMA (Time Division Multiple Access) power variation and the more smooth, slowly varying CDMA signal (Code Division Multiple Access). Epidemiological and biological studies must reflect the intricate details of the various systems. (author)

  4. Effect of the Route of Administration and PEGylation of Poly(amidoamine) Dendrimers on Their Systemic and Lung Cellular Biodistribution.

    Science.gov (United States)

    Zhong, Qian; Merkel, Olivia M; Reineke, Joshua J; da Rocha, Sandro R P

    2016-06-01

    There are many opportunities in the development of oral inhalation (oi) formulations for the delivery of small molecule therapeutics and biologics to and through the lungs. Nanocarriers have the potential to play a key role in advancing oi technologies and pushing the boundary of the pulmonary delivery market. In this work we investigate the effect of the route of administration and PEGylation on the systemic and lung cellular biodistribution of generation 3, amino-terminated poly(amidoamine) (PAMAM) dendrimers (G3NH2). Pharmacokinetic profiles show that the dendrimers reach their peak concentration in systemic circulation within a few hours after pulmonary delivery, independent of their chemistry (PEGylated or not), charge (+24 mV for G3NH2 vs -3.7 mV for G3NH2-24PEG1000), or size (5.1 nm for G3NH2 and 9.9 nm for G3NH2-24PEG1000). However, high density of surface modification with PEG enhances pulmonary absorption and the peak plasma concentration upon pulmonary delivery. The route of administration and PEGylation also significantly impact the whole body and local (lung cellular) distribution of the dendrimers. While ca. 83% of G3NH2 is found in the lungs upon pulmonary delivery at 6.5 h post administration, only 2% reached the lungs upon intravenous (iv) delivery. Moreover, no measurable concentration of either G3NH2 or G3NH2-24PEG1000 is found in the lymph nodes upon iv administration, while these are the tissues with the second highest mass distribution of dendrimers post pulmonary delivery. Dendrimer chemistry also significantly impacts the (cellular) distribution of the nanocarriers in the lung tissue. Upon pulmonary delivery, approximately 20% of the lung endothelial cells are seen to internalize G3NH2-24PEG1000, compared to only 6% for G3NH2. Conversely, G3NH2 is more readily taken up by lung epithelial cells (35%) when compared to its PEGylated counterpart (24%). The results shown here suggest that both the pulmonary route of administration and dendrimer

  5. Micro electrochemical sensors and PCR systems: cellular and molecular tools for wine yeast analysis

    OpenAIRE

    Ress, Cristina

    2010-01-01

    Nowadays, exciting bioanalytical microsystems are currently receiving increasing attention in biology since they can comply with the considerable demand for reliable, sensitive and low-cost analysis tools. Small reagents volumes, low power consumption, portability, fast analysis, high throughput and systems integration are the key aspects that make these systems more and more appealing within both the academic and industrial communities. In the last years, many microdevices were developed for...

  6. Error Performance Analysis to Increase Capacity of A Cellular System Using SDMA

    OpenAIRE

    Md. M. Hossain; Hossain, J.

    2010-01-01

    One of the biggest drawbacks of the wireless environment is the limited bandwidth. However, the users sharing this limited bandwidth have been increasing considerably.Space Division Multiple Access (SDMA) is a new technology by which the capacity of existing mobile communication systems can economically be increased. This paper has been presented how the capacity can be enhanced by using SDMA with smart antennas in mobile communications system. Based on Adaptive Antenna Array (AAA) technology...

  7. Enhanced Cellular Delivery and Biocompatibility of a Small Layered Double Hydroxide–Liposome Composite System

    Directory of Open Access Journals (Sweden)

    Haiyan Dong

    2014-11-01

    Full Text Available The various classes of gene delivery vectors possess distinct advantages and disadvantages, each of which impacts on cargo loading, delivery and, ultimately, its function. With this in mind, herein we report on a small layered double hydroxide (sLDH–liposome composite system, drawing upon the salient features of LDH and liposome classes of vectors, while avoiding their inherent shortfalls when used independently. sLDH–liposome composites were prepared by the hydration of freeze-dried matrix method. These composite systems, with a Z-average size of ≈200 nm, exhibited low cytotoxicity and demonstrated good suspension stability, both in water and cell culture medium after rehydration. Our studies demonstrate that short dsDNAs/ssDNAs were completely bound and protected in the composite system at an sLDH:DNA mass ratio of 20:1, regardless of the approach to DNA loading. This composite system delivered DNA to HCT-116 cells with ≈3-fold greater efficiency, when compared to sLDH alone. Our findings point towards the sLDH-liposome composite system being an effective and biocompatible gene delivery system.

  8. Characterization of a novel bioreactor system for 3D cellular mechanobiology studies.

    Science.gov (United States)

    Cook, Colin A; Huri, Pinar Y; Ginn, Brian P; Gilbert-Honick, Jordana; Somers, Sarah M; Temple, Joshua P; Mao, Hai-Quan; Grayson, Warren L

    2016-08-01

    In vitro engineering systems can be powerful tools for studying tissue development in response to biophysical stimuli as well as for evaluating the functionality of engineered tissue grafts. It has been challenging, however, to develop systems that adequately integrate the application of biomimetic mechanical strain to engineered tissue with the ability to assess functional outcomes in real time. The aim of this study was to design a bioreactor system capable of real-time conditioning (dynamic, uniaxial strain, and electrical stimulation) of centimeter-long 3D tissue engineered constructs simultaneously with the capacity to monitor local strains. The system addresses key limitations of uniform sample loading and real-time imaging capabilities. Our system features an electrospun fibrin scaffold, which exhibits physiologically relevant stiffness and uniaxial alignment that facilitates cell adhesion, alignment, and proliferation. We have demonstrated the capacity for directly incorporating human adipose-derived stromal/stem cells into the fibers during the electrospinning process and subsequent culture of the cell-seeded constructs in the bioreactor. The bioreactor facilitates accurate pre-straining of the 3D constructs as well as the application of dynamic and static uniaxial strains while monitoring bulk construct tensions. The incorporation of fluorescent nanoparticles throughout the scaffolds enables in situ monitoring of local strain fields using fluorescent digital image correlation techniques, since the bioreactor is imaging compatible, and allows the assessment of local sample stiffness and stresses when coupled with force sensor measurements. In addition, the system is capable of measuring the electromechanical coupling of skeletal muscle explants by applying an electrical stimulus and simultaneously measuring the force of contraction. The packaging of these technologies, biomaterials, and analytical methods into a single bioreactor system has produced a

  9. Regulation of autophagy in oxygen-dependent cellular stress.

    Science.gov (United States)

    Ryter, Stefan W; Choi, Augustine M K

    2013-01-01

    Oxidative stress caused by supraphysiological production of reactive oxygen species (ROS), can cause cellular injury associated with protein and lipid oxidation, DNA damage, and mitochondrial dysfunction. The cellular responses triggered by oxidative stress include the altered regulation of signaling pathways that culminate in the regulation of cell survival or cell death pathways. Recent studies suggest that autophagy, a cellular homeostatic process that governs the turnover of damaged organelles and proteins, may represent a general cellular and tissue response to oxidative stress. The autophagic pathway involves the encapsulation of substrates in double-membraned vesicles, which are subsequently delivered to the lysosome for enzymatic degradation and recycling of metabolic precursors. Autophagy may play multifunctional roles in cellular adaptation to stress, by maintaining mitochondrial integrity, and removing damaged proteins. Additionally, autophagy may play important roles in the regulation of inflammation and immune function. Modulation of the autophagic pathway has been reported in cell culture models of oxidative stress, including altered states of oxygen tension (i.e., hypoxia, hyperoxia), and exposure to oxidants. Furthermore, proteins that regulate autophagy may be subject to redox regulation. The heme oxygenase- 1 (HO)-1 enzyme system may have a role in the regulation of autophagy. Recent studies suggest that carbon monoxide (CO), a reaction product of HO activity which can alter mitochondrial function, may induce autophagy in cultured epithelial cells. In conclusion, current research suggests a central role for autophagy as a mammalian oxidative stress response and its interrelationship to other stress defense systems. PMID:23092322

  10. Plants versus Fungi and Oomycetes: Pathogenesis, Defense and Counter-Defense in the Proteomics Era

    Directory of Open Access Journals (Sweden)

    Abdelbasset El Hadrami

    2012-06-01

    Full Text Available Plant-fungi and plant-oomycete interactions have been studied at the proteomic level for many decades. However, it is only in the last few years, with the development of new approaches, combined with bioinformatics data mining tools, gel staining, and analytical instruments, such as 2D-PAGE/nanoflow-LC-MS/MS, that proteomic approaches thrived. They allow screening and analysis, at the sub-cellular level, of peptides and proteins resulting from plants, pathogens, and their interactions. They also highlight post-translational modifications to proteins, e.g., glycosylation, phosphorylation or cleavage. However, many challenges are encountered during in planta studies aimed at stressing details of host defenses and fungal and oomycete pathogenicity determinants during interactions. Dissecting the mechanisms of such host-pathogen systems, including pathogen counter-defenses, will ensure a step ahead towards understanding current outcomes of interactions from a co-evolutionary point of view, and eventually move a step forward in building more durable strategies for management of diseases caused by fungi and oomycetes. Unraveling intricacies of more complex proteomic interactions that involve additional microbes, i.e., PGPRs and symbiotic fungi, which strengthen plant defenses will generate valuable information on how pathosystems actually function in nature, and thereby provide clues to solving disease problems that engender major losses in crops every year.

  11. Defense Mechanisms: A Bibliography.

    Science.gov (United States)

    Pedrini, D. T.; Pedrini, Bonnie C.

    This bibliography includes studies of defense mechanisms, in general, and studies of multiple mechanisms. Defense mechanisms, briefly and simply defined, are the unconscious ego defendants against unpleasure, threat, or anxiety. Sigmund Freud deserves the clinical credit for studying many mechanisms and introducing them in professional literature.…

  12. Stress, ageing and their influence on functional, cellular and molecular aspects of the immune system.

    Science.gov (United States)

    Vitlic, Ana; Lord, Janet M; Phillips, Anna C

    2014-06-01

    The immune response is essential for keeping an organism healthy and for defending it from different types of pathogens. It is a complex system that consists of a large number of components performing different functions. The adequate and controlled interaction between these components is necessary for a robust and strong immune response. There are, however, many factors that interfere with the way the immune response functions. Stress and ageing now consistently appear in the literature as factors that act upon the immune system in the way that is often damaging. This review focuses on the role of stress and ageing in altering the robustness of the immune response first separately, and then simultaneously, discussing the effects that emerge from their interplay. The special focus is on the psychological stress and the impact that it has at different levels, from the whole system to the individual molecules, resulting in consequences for physical health. PMID:24562499

  13. CELLULAR AND MOLECULAR BIOMARKERS AND POTENTIAL THERAPEUTIC TARGETS IN SYSTEMIC LUPUS ERYTHEMATOSUS

    Directory of Open Access Journals (Sweden)

    A. A. Mesnyankina

    2016-01-01

    Full Text Available Systemic lupus erythematosus (SLE is one of the most severe and prognostically poor systemic connective tissue diseases that affect mainly women of childbearing age. The recent researches have provided a deeper insight into its pathogenesis, the identification and definition of a role of cytokines, cells, and intercellular bonds involved in the development of SLE. These data are borne in mind when designing novel biological agents, the action of which is aimed at inhibiting the targets implicated in the development of the disease. This information also may provide better understanding the mechanism of action of drugs, such as belimumab and rituximab, which have already proven their efficacy. 

  14. Penitentiary integral defense

    Directory of Open Access Journals (Sweden)

    Francisco Javier Rodríguez Román

    2012-07-01

    Full Text Available Penitentiary integral defense is a set of techniques designed to solve the breach of the peace that may appear in the penitentiary world. This paper studies the scope and legal framework of penitentiary self-defense. This kind of self-defense is defined by the particular characteristics of a confinement context and a very specific regulation aimed at preserving the integrity of prisoners and penitentiary staff as well as prison facilities. A technical section illustrating the main penitentiary integral defense’s fundamentals and methods is also included. The defense methods showed here are: frisking, confinement cell entry, prisoner transfer, active resistance, aggressions between prisoners, standard defense equipment, handcuffs use, and techniques against holds, non-armed attacks and attacks with an edged weapon.

  15. Nano-jewels in biology. Gold and platinum on diamond nanoparticles as antioxidant systems against cellular oxidative stress.

    Science.gov (United States)

    Martín, Roberto; Menchón, Cristina; Apostolova, Nadezda; Victor, Victor M; Alvaro, Mercedes; Herance, José Raúl; García, Hermenegildo

    2010-11-23

    Diamond nanoparticles (DNPs) obtained by explosive detonation have become commercially available. These commercial DNPs can be treated under Fenton conditions (FeSO(4) and H(2)O(2) at acidic pH) to obtain purer DNP samples with a small average particle size (4 nm) and a large population of surface OH groups (HO-DNPs). These Fenton-treated HO-DNPs have been used as a support of gold and platinum nanoparticles (≤2 nm average size). The resulting materials (Au/HO-DNP and Pt/HO-DNP) exhibit a high antioxidant activity against reactive oxygen species induced in a hepatoma cell line. In addition to presenting good biocompatibility, Au/HO- and Pt/HO-DNP exhibit about a two-fold higher antioxidant activity than glutathione, one of the reference antioxidant systems. The most active material against cellular oxidative stress was Au/HO-DNP. PMID:20939514

  16. Performance Evaluation of WiMAX Broadband from High Altitude Platform Cellular System and Terrestrial Coexistence Capability

    Directory of Open Access Journals (Sweden)

    T. Hult

    2008-12-01

    Full Text Available The performance obtained from providing worldwide interoperability for microwave access (WiMAX from high altitude platforms (HAPs with multiple antenna payloads is investigated, and the coexistence capability with multiple-operator terrestrial WiMAX deployments is examined. A scenario composed of a single HAP and coexisting multiple terrestrial WiMAX base stations deployed inside the HAP coverage area (with radius of 30 km to provide services to fixed users with the antenna mounted on the roof with a directive antenna to receive signals from HAPs is proposed. A HAP cellular configuration with different possible reuse patterns is established. The coexistence performance is assessed in terms of HAP downlink and uplink performance, interfered by terrestrial WiMAX deployment. Simulation results show that it is effective to deliver WiMAX via HAPs and share the spectrum with terrestrial systems.

  17. The regeneration of epidermal cells of Saintpaulia leaves as a new plant-tissue system for cellular radiation biology.

    Science.gov (United States)

    Engels, F M; van der Laan, F M; Leenhouts, H P; Chadwick, K H

    1980-09-01

    Investigation of the nucleus of epidermal cells of the petioles of Saintpaulia leaves by cytofluorimetry revealed that all cells are in a non-cycling pre DNA synthesis phase. Cultivation of dissected leaves results in a synchronous regeneration process of a defined number of cells. Five days after onset of cultivation the cells reach the first mitosis. The nuclear development during the regeneration process is described. Irradiation of the leaves results in a directly visible inhibition of this regenerating capability which is used to quantify cell survival in a tissue. The data show that the radiation response has a similar shape to that of the survival of single cells in culture. This response can be observed before the first mitosis of the cells and its application as a new plant tissue system for cellular radiation research is discussed. PMID:7012060

  18. Effects of cadmium on ultrastructure and antioxidative defense system in hyperaccumulator and non-hyperaccumulator ecotypes of Sedum alfredii Hance.

    Science.gov (United States)

    Jin, Xiaofen; Yang, Xiaoe; Islam, Ejazul; Liu, Dan; Mahmood, Qaisar

    2008-08-15

    Plant growth, ultrastructural and antioxidant adaptations and glutathione biosynthesis in Cd-hyperaccumulating ecotype Sedum alfredii Hance (HE) countering high Cd environment were investigated and compared with its non Cd-hyperaccumulating ecotype (NHE). Cadmium exposure resulted in significant ultrastructural changes in root meristem and leaf mesophyll cells of S. alfredii, but damage was more pronounced in NHE even when Cd concentrations were one-tenth of those applied to HE. Cadmium stress damaged chloroplasts causing imbalanced lamellae formation coupled with early leaf senescence. Histochemical results revealed that glutathione (GSH) biosynthesis inhibition led to overproduction of hydrogen peroxide (H(2)O(2)) and superoxide radical (O(2)(*-)) in HE but not in NHE. Differences were noted in both HE and NHE for catalase (CAT), guaiacol peroxidase (GPX), ascorbate peroxidase (APX) and glutathione reductase (GR) activities under various Cd stress levels. No relationship was found between antioxidative defense capacity including activities of superoxide dismutase (SOD), CAT, GPX, APX and GR as well as ascorbic acid (AsA) contents and Cd tolerance in the two ecotypes of S. alfredii. The GSH biosynthesis induction in root and shoot exposed to elevated Cd conditions may be involved in Cd tolerance and hyperaccumulation in HE of S. alfredii H. PMID:18242844

  19. Effects of cadmium on ultrastructure and antioxidative defense system in hyperaccumulator and non-hyperaccumulator ecotypes of Sedum alfredii Hance

    Energy Technology Data Exchange (ETDEWEB)

    Jin Xiaofen [Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, Zhejiang University, Hangzhou 310029 (China); Zhejiang Provincial Key Lab of Subtropical Soil Science and Plant Nutrition, College of Environmental and Natural Resources Science, Zhejiang University, Hangzhou 310029 (China); Yang Xiaoe [Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, Zhejiang University, Hangzhou 310029 (China); Zhejiang Provincial Key Lab of Subtropical Soil Science and Plant Nutrition, College of Environmental and Natural Resources Science, Zhejiang University, Hangzhou 310029 (China)], E-mail: xyang@zju.edu.cn; Islam, Ejazul [Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, Zhejiang University, Hangzhou 310029 (China); Zhejiang Provincial Key Lab of Subtropical Soil Science and Plant Nutrition, College of Environmental and Natural Resources Science, Zhejiang University, Hangzhou 310029 (China); Nuclear Institute of Agriculture, Tandojam 48800, Hyderabad (Pakistan); Liu Dan [School of Tourism and Health, Zhejiang Forestry College, 311300 Lin' an (China); Mahmood, Qaisar [Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, Zhejiang University, Hangzhou 310029 (China); Zhejiang Provincial Key Lab of Subtropical Soil Science and Plant Nutrition, College of Environmental and Natural Resources Science, Zhejiang University, Hangzhou 310029 (China)

    2008-08-15

    Plant growth, ultrastructural and antioxidant adaptations and glutathione biosynthesis in Cd-hyperaccumulating ecotype Sedum alfredii Hance (HE) countering high Cd environment were investigated and compared with its non Cd-hyperaccumulating ecotype (NHE). Cadmium exposure resulted in significant ultrastructural changes in root meristem and leaf mesophyll cells of S. alfredii, but damage was more pronounced in NHE even when Cd concentrations were one-tenth of those applied to HE. Cadmium stress damaged chloroplasts causing imbalanced lamellae formation coupled with early leaf senescence. Histochemical results revealed that glutathione (GSH) biosynthesis inhibition led to overproduction of hydrogen peroxide (H{sub 2}O{sub 2}) and superoxide radical (O{sub 2}{center_dot}{sup -}) in HE but not in NHE. Differences were noted in both HE and NHE for catalase (CAT), guaiacol peroxidase (GPX), ascorbate peroxidase (APX) and glutathione reductase (GR) activities under various Cd stress levels. No relationship was found between antioxidative defense capacity including activities of superoxide dismutase (SOD), CAT, GPX, APX and GR as well as ascorbic acid (AsA) contents and Cd tolerance in the two ecotypes of S. alfredii. The GSH biosynthesis induction in root and shoot exposed to elevated Cd conditions may be involved in Cd tolerance and hyperaccumulation in HE of S. alfredii H.

  20. Effects of cadmium on ultrastructure and antioxidative defense system in hyperaccumulator and non-hyperaccumulator ecotypes of Sedum alfredii Hance

    International Nuclear Information System (INIS)

    Plant growth, ultrastructural and antioxidant adaptations and glutathione biosynthesis in Cd-hyperaccumulating ecotype Sedum alfredii Hance (HE) countering high Cd environment were investigated and compared with its non Cd-hyperaccumulating ecotype (NHE). Cadmium exposure resulted in significant ultrastructural changes in root meristem and leaf mesophyll cells of S. alfredii, but damage was more pronounced in NHE even when Cd concentrations were one-tenth of those applied to HE. Cadmium stress damaged chloroplasts causing imbalanced lamellae formation coupled with early leaf senescence. Histochemical results revealed that glutathione (GSH) biosynthesis inhibition led to overproduction of hydrogen peroxide (H2O2) and superoxide radical (O2·-) in HE but not in NHE. Differences were noted in both HE and NHE for catalase (CAT), guaiacol peroxidase (GPX), ascorbate peroxidase (APX) and glutathione reductase (GR) activities under various Cd stress levels. No relationship was found between antioxidative defense capacity including activities of superoxide dismutase (SOD), CAT, GPX, APX and GR as well as ascorbic acid (AsA) contents and Cd tolerance in the two ecotypes of S. alfredii. The GSH biosynthesis induction in root and shoot exposed to elevated Cd conditions may be involved in Cd tolerance and hyperaccumulation in HE of S. alfredii H

  1. Application of the Prunus spp. Cyanide Seed Defense System onto Wheat: Reduced Insect Feeding and Field Growth Tests.

    Science.gov (United States)

    Mora, Carlos A; Halter, Jonas G; Adler, Cornel; Hund, Andreas; Anders, Heidrun; Yu, Kang; Stark, Wendelin J

    2016-05-11

    Many crops are ill-protected against insect pests during storage. To protect cereal grains from herbivores during storage, pesticides are often applied. While pesticides have an undoubtable functionality, increasing concerns are arising about their application. In the present study, we investigated a bioinspired cyanogenic grain coating with amygdalin as cyanogenic precursor mimicking the feeding-triggered release of hydrogen cyanide (HCN) found for example in bitter almonds. The multilayer coating consisted of biodegradable polylactic acid with individual layers containing amygdalin or β-glucosidase which is capable of degrading amygdalin to HCN. This reaction occurred only when the layers were ruptured, e.g., by a herbivore attack. Upon feeding coated cyanogenic wheat grains to Tenebrio molitor (mealworm beetle), Rhizopertha dominica (lesser grain borer), and Plodia interpunctella (Indianmeal moth), their reproduction as well as consumption rate were significantly reduced, whereas germination ability increased compared to noncoated grains. In field experiments, we observed an initial growth delay compared to uncoated grains which became negligible at later growth stages. The here shown strategy to artificially apply a naturally occurring defense mechanisms could be expanded to other crops than wheat and has the potential to replace certain pesticides with the benefit of complete biodegradability and increased safety during storage. PMID:27119432

  2. GIS Based System for Post-Earthquake Crisis Managment Using Cellular Network

    Science.gov (United States)

    Raeesi, M.; Sadeghi-Niaraki, A.

    2013-09-01

    Earthquakes are among the most destructive natural disasters. Earthquakes happen mainly near the edges of tectonic plates, but they may happen just about anywhere. Earthquakes cannot be predicted. Quick response after disasters, like earthquake, decreases loss of life and costs. Massive earthquakes often cause structures to collapse, trapping victims under dense rubble for long periods of time. After the earthquake and destroyed some areas, several teams are sent to find the location of the destroyed areas. The search and rescue phase usually is maintained for many days. Time reduction for surviving people is very important. A Geographical Information System (GIS) can be used for decreasing response time and management in critical situations. Position estimation in short period of time time is important. This paper proposes a GIS based system for post-earthquake disaster management solution. This system relies on several mobile positioning methods such as cell-ID and TA method, signal strength method, angel of arrival method, time of arrival method and time difference of arrival method. For quick positioning, the system can be helped by any person who has a mobile device. After positioning and specifying the critical points, the points are sent to a central site for managing the procedure of quick response for helping. This solution establishes a quick way to manage the post-earthquake crisis.

  3. GIS BASED SYSTEM FOR POST-EARTHQUAKE CRISIS MANAGMENT USING CELLULAR NETWORK

    Directory of Open Access Journals (Sweden)

    M. Raeesi

    2013-09-01

    Full Text Available Earthquakes are among the most destructive natural disasters. Earthquakes happen mainly near the edges of tectonic plates, but they may happen just about anywhere. Earthquakes cannot be predicted. Quick response after disasters, like earthquake, decreases loss of life and costs. Massive earthquakes often cause structures to collapse, trapping victims under dense rubble for long periods of time. After the earthquake and destroyed some areas, several teams are sent to find the location of the destroyed areas. The search and rescue phase usually is maintained for many days. Time reduction for surviving people is very important. A Geographical Information System (GIS can be used for decreasing response time and management in critical situations. Position estimation in short period of time time is important. This paper proposes a GIS based system for post–earthquake disaster management solution. This system relies on several mobile positioning methods such as cell-ID and TA method, signal strength method, angel of arrival method, time of arrival method and time difference of arrival method. For quick positioning, the system can be helped by any person who has a mobile device. After positioning and specifying the critical points, the points are sent to a central site for managing the procedure of quick response for helping. This solution establishes a quick way to manage the post–earthquake crisis.

  4. Cloud computing models and their application in LTE based cellular systems

    NARCIS (Netherlands)

    Staring, A.J.; Karagiannis, G.

    2013-01-01

    As cloud computing emerges as the next novel concept in computer science, it becomes clear that the model applied in large data storage systems used to resolve issues coming forth from an increasing demand, could also be used to resolve the very high bandwidth requirements on access network, core ne

  5. Phagocytic defense in the lung.

    Science.gov (United States)

    Reynolds, H Y

    1985-01-01

    immunizing antigen, type of adjuvant and site of administration will be required. Finally, selective immunization of the mucosal surfaces of the airways will present different challenges than parenteral methods that elicit systemic responses and may not be satisfactory to prevent certain respiratory infections. The prospects are exciting despite much work in cellular immunology that remains for the future. PMID:3890719

  6. Analytical tools for the study of cellular glycosylation in the immune system

    Directory of Open Access Journals (Sweden)

    Yvette eVan Kooyk

    2013-12-01

    Full Text Available It is becoming increasingly clear that glycosylation plays important role in intercellular communication within the immune system. Glycosylation-dependent interactions are crucial for the innate and adaptive immune system and regulate immune cell trafficking, synapse formation, activation, and survival. These functions take place by the cis or trans interaction of lectins with glycans. Classical immunological and biochemical methods have been used for the study of lectin function; however, the investigation of their counterparts, glycans, requires very specialized methodologies that have been extensively developed in the past decade within the Glycobiology scientific community. This Mini-Review intends to summarize the available technology for the study of glycan biosynthesis, its regulation and characterization for their application to the study of glycans in Immunology.

  7. In Vitro Testing of Biomaterials for Neural Repair: Focus on Cellular Systems and High-Content Analysis.

    Science.gov (United States)

    Baldassarro, Vito Antonio; Dolci, Luisa Stella; Mangano, Chiara; Giardino, Luciana; Gualandi, Chiara; Focarete, Maria Letizia; Calzà, Laura

    2016-01-01

    Biomimetic materials are designed to stimulate specific cellular responses at the molecular level. To improve the soundness of in vitro testing of the biological impact of new materials, appropriate cell systems and technologies must be standardized also taking regulatory issues into consideration. In this study, the biological and molecular effects of different scaffolds on three neural systems, that is, the neural cell line SH-SY5Y, primary cortical neurons, and neural stem cells, were compared. The effect of poly(L-lactic acid) scaffolds having different surface geometry (conventional two-dimensional seeding flat surface, random or aligned fibers as semi3D structure) and chemical functionalization (laminin or ECM extract) were studied. The endpoints were defined for efficacy (i.e., neural differentiation and neurite elongation) and for safety (i.e., cell death/survival) using high-content analysis. It is demonstrated that (i) the definition of the biological properties of biomaterials is profoundly influenced by the test system used; (ii) the definition of the in vitro safety profile of biomaterials for neural repair is also influenced by the test system; (iii) cell-based high-content screening may well be successfully used to characterize both the efficacy and safety of novel biomaterials, thus speeding up and improving the soundness of this critical step in material science having medical applications. PMID:27588220

  8. Cellular Localization of Aquaporin-1 in the Human and Mouse Trigeminal Systems

    OpenAIRE

    Gao, Junying; Tan, Meiyun; Gu, Minxia; Marshall, Charles; Ding, Jiong; Hu, Gang; Xiao, Ming

    2012-01-01

    Previous studies reported that a subpopulation of mouse and rat trigeminal neurons express water channel aquaporin-1 (AQP1). In this study we make a comparative investigation of AQP1 localization in the human and mouse trigeminal systems. Immunohistochemistry and immunofluorescence results showed that AQP1 was localized to the cytoplasm and cell membrane of some medium and small-sized trigeminal neurons. Additionally, AQP1 was found in numerous peripheral trigeminal axons of humans and mice. ...

  9. Cellular automata for the spreading of technologies in socio-economic systems

    OpenAIRE

    Kun, Ferenc; Kocsis, Gergely; Farkas, Janos

    2008-01-01

    We introduce an agent-based model for the spreading of technological developments in socio-economic systems where the technology is mainly used for the collaboration/interaction of agents. Agents use products of different technologies to collaborate with each other which induce costs proportional to the difference of technological levels. Additional costs arise when technologies of different providers are used. Agents can adopt technologies and providers of their interacting partners in order...

  10. Tinnitus: pathology of synaptic plasticity at the cellular and system levels.

    Science.gov (United States)

    Guitton, Matthieu J

    2012-01-01

    Despite being more and more common, and having a high impact on the quality of life of sufferers, tinnitus does not yet have a cure. This has been mostly the result of limited knowledge of the biological mechanisms underlying this adverse pathology. However, the last decade has witnessed tremendous progress in our understanding on the pathophysiology of tinnitus. Animal models have demonstrated that tinnitus is a pathology of neural plasticity, and has two main components: a molecular, peripheral component related to the initiation phase of tinnitus; and a system-level, central component-related to the long-term maintenance of tinnitus. Using the most recent experimental data and the molecular/system dichotomy as a framework, we describe here the biological basis of tinnitus. We then discuss these mechanisms from an evolutionary perspective, highlighting similarities with memory. Finally, we consider how these discoveries can translate into therapies, and we suggest operative strategies to design new and effective combined therapeutic solutions using both pharmacological (local and systemic) and behavioral tools (e.g., using tele-medicine and virtual reality settings). PMID:22408611

  11. Isonitrosoacetophenone drives transcriptional reprogramming in Nicotiana tabacum cells in support of innate immunity and defense.

    Directory of Open Access Journals (Sweden)

    Arnaud T Djami-Tchatchou

    Full Text Available Plants respond to various stress stimuli by activating broad-spectrum defense responses both locally as well as systemically. As such, identification of expressed genes represents an important step towards understanding inducible defense responses and assists in designing appropriate intervention strategies for disease management. Genes differentially expressed in tobacco cell suspensions following elicitation with isonitrosoacetophenone (INAP were identified using mRNA differential display and pyro-sequencing. Sequencing data produced 14579 reads, which resulted in 198 contigs and 1758 singletons. Following BLAST analyses, several inducible plant defense genes of interest were identified and classified into functional categories including signal transduction, transcription activation, transcription and protein synthesis, protein degradation and ubiquitination, stress-responsive, defense-related, metabolism and energy, regulation, transportation, cytoskeleton and cell wall-related. Quantitative PCR was used to investigate the expression of 17 selected target genes within these categories. Results indicate that INAP has a sensitising or priming effect through activation of salicylic acid-, jasmonic acid- and ethylene pathways that result in an altered transcriptome, with the expression of genes involved in perception of pathogens and associated cellular re-programming in support of defense. Furthermore, infection assays with the pathogen Pseudomonas syringae pv. tabaci confirmed the establishment of a functional anti-microbial environment in planta.

  12. Cellular and Network Mechanisms Underlying Information Processing in a Simple Sensory System

    Science.gov (United States)

    Jacobs, Gwen; Henze, Chris; Biegel, Bryan (Technical Monitor)

    2002-01-01

    Realistic, biophysically-based compartmental models were constructed of several primary sensory interneurons in the cricket cercal sensory system. A dynamic atlas of the afferent input to these cells was used to set spatio-temporal parameters for the simulated stimulus-dependent synaptic inputs. We examined the roles of dendritic morphology, passive membrane properties, and active conductances on the frequency tuning of the neurons. The sensitivity of narrow-band low pass interneurons could be explained entirely by the electronic structure of the dendritic arbors and the dynamic sensitivity of the SIZ. The dynamic characteristics of interneurons with higher frequency sensitivity required models with voltage-dependent dendritic conductances.

  13. Complex Systems in APL: Fractals, Evolving Cellular Automata and Artificial Life

    OpenAIRE

    Alfonseca, Manuel; Ortega, Alfonso; Cruz Echeandía, Marina de la

    2002-01-01

    This is the author's version of the work. It is posted here for your personal use. Not for redistribution. The definitive Version of Record was published in APL Quote Quad 32.4 (2002): 17 – 26, http://dx.doi.org/10.1145/604444.602233 We have been working for several years on the representation, study and simulation of complex systems by means of formal methods. APL2 and other programming languages have been used to develop our tools and experiments. This paper summarizes our APL2 works on ...

  14. An Inducible System for Rapid Degradation of Specific Cellular Proteins Using Proteasome Adaptors

    Science.gov (United States)

    Wilmington, Shameika R.; Matouschek, Andreas

    2016-01-01

    A common way to study protein function is to deplete the protein of interest from cells and observe the response. Traditional methods involve disrupting gene expression but these techniques are only effective against newly synthesized proteins and leave previously existing and stable proteins untouched. Here, we introduce a technique that induces the rapid degradation of specific proteins in mammalian cells by shuttling the proteins to the proteasome for degradation in a ubiquitin-independent manner. We present two implementations of the system in human culture cells that can be used individually to control protein concentration. Our study presents a simple, robust, and flexible technology platform for manipulating intracellular protein levels. PMID:27043013

  15. Characterization of the emergent properties of a synthetic quasi-cellular system

    Directory of Open Access Journals (Sweden)

    Lazzerini-Ospri Lorenzo

    2012-03-01

    Full Text Available Abstract Background The process of solutes entrapment during liposomes formation is interesting for the investigation of the relationship between the formation of compartments and the distribution of molecules inside them; a relevant issue in the studies of the origin of life. Theoretically, when no interactions are supposed among the chemical species to be entrapped, the entrapment is described by a standard Poisson process. But very recent experimental findings show that, for small liposomes (100 nm diameter, the distribution of entrapped molecules is best described by a power-law function. This is of a great importance, as the two random processes give rise to two completely different scenarios. Here we present an in silico stochastic simulation of the encapsulation of a cell-free molecular translation system (the PURE system, obtained following two different entrapment models: a pure Poisson process, and a power-law. The protein synthesis inside the liposomes has been studied in both cases, with the aim to highlight experimental observables that could be measured to assess which model gives a better representation of the real process. Results Firstly, a minimal model for in vitro protein synthesis, based on the PURE system molecular composition, has been formalized. Then, we have designed a reliable experimental simulation where stochastic factors affect the reaction course inside the compartment. To this end, 24 solutes, which represent the PURE system components, have been stochastically distributed among vesicles by following either a Poisson or a power-law distribution. The course of the protein synthesis within each vesicle has been consequently calculated, as a function of vesicle size. Our study can predict translation yield in a population of small liposomes down to the attoliter (10-18 L range. Our results show that the efficiency of protein synthesis peaks at approximately 3·10-16 L (840 nm diam. with a Poisson distribution of

  16. Combined herbicide and saline stress differentially modulates hormonal regulation and antioxidant defense system in Oryza sativa cultivars.

    Science.gov (United States)

    Islam, Faisal; Ali, Basharat; Wang, Jian; Farooq, Muhammad A; Gill, Rafaqat A; Ali, Shafaqat; Wang, Danying; Zhou, Weijun

    2016-10-01

    Plants are simultaneously exposed to a combination of biotic and abiotic stresses in field conditions. Crops respond to the combined stress in a unique way which cannot be understood by extrapolating the results of individual stress. In the present study, effects of individual and combined stress of herbicide (2,4-dichlorophenoxyacetic acid) and salinity (NaCl) on two Oryza sativa cultivars (ZJ 88 and XS 134) were investigated. Both herbicide and saline stress affected the plant growth differentially and produced oxidative stress in rice cultivars. Interestingly, the combination of herbicide and salinity showed a significant protection to both rice cultivars by reducing ROS (H2O2, O2(-)) and lipid peroxidation through modulation of enzymatic (SOD, POD, CAT and APX) and non-enzymatic (TSP, sugars, phenolic and proline) antioxidants. In addition, active regulation of transcript levels of genes encoding Na(+) and K(+) (OsHKT1;5, OsLti6a,b, OsHKT2;1, OsSOS1, OsCNGC1, OsNHX1 and OsAKT1) transporter proteins reduced sodium and enhanced potassium accumulation under combined stress, resulted a better growth and ionic homeostasis in both rice cultivars. The production of ABA and IAA was significantly higher in cultivar XS 134 compared to cultivar ZJ 88 under control conditions. However, combined herbicide and saline stress enhanced the accumulation of phytohormones (IAA and ABA) and transcription of ethylene in cultivar ZJ 88, which might be one of the factors responsible for poor salt tolerance in sensitive cultivar. These findings indicated that herbicide application under saline stress confers tolerance to salinity in rice cultivars, likely by reducing oxidative damage, modulating mineral absorption, upgradation of antioxidant defense and by dynamic regulation of key genes involved in Na(+) and K(+) homeostasis in plants. PMID:27258572

  17. Polyhydroxylated fullerene attenuates oxidative stress-induced apoptosis via a fortifying Nrf2-regulated cellular antioxidant defence system

    Directory of Open Access Journals (Sweden)

    Ye SF

    2014-04-01

    Full Text Available Shefang Ye,1 Min Chen,1 Yuanqin Jiang,1,2 Mingliang Chen,3 Tong Zhou,1 Yange Wang,1 Zhenqing Hou,1 Lei Ren11Department of Biomaterials, Research Center of Biomedical Engineering, College of Materials, Xiamen University, Xiamen, People's Republic of China; 2First Affiliated Hospital of Xiamen University, Xiamen, People's Republic of China; 3Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen, People's Republic of ChinaAbstract: Polyhydroxylated derivatives of fullerene C60, named fullerenols (C60[OH]n, have stimulated great interest because of their potent antioxidant properties in various chemical and biological systems, which enable them to be used as a new promising pharmaceutical for the future treatment of oxidative stress-related diseases, but the details remain unknown. Nuclear factor erythroid 2-related factor 2 (Nrf2 is a principal transcription factor that regulates expression of several antioxidant genes via binding to the antioxidant response element and plays a crucial role in cellular defence against oxidative stress. In this study we investigated whether activation of the Nrf2/antioxidant response element pathway contributes to the cytoprotective effects of C60(OH24. Our results showed that C60(OH24 enhanced nuclear translocation of Nrf2 and upregulated expression of phase II antioxidant enzymes, including heme oxygenase-1 (HO-1, NAD(PH: quinine oxidoreductase 1, and γ-glutamate cysteine ligase in A549 cells. Treatment with C60(OH24 resulted in phosphorylation of p38 mitogen-activated protein kinases (p38 MAPK, extracellular signal-regulated kinases, and c-Jun-N-terminal kinases. By using inhibitors of cellular kinases, we showed that pretreatment of A549 cells with SB203580, a specific inhibitor of p38 MAPK, abolished nuclear translocation of Nrf2 and induction of HO-1 protein induced by C60(OH24, indicating an involvement of p38 MAPK in Nrf2/HO-1 activation by C60

  18. Current concepts concerning radioprotective and cryoprotective properties of dimethyl sulfoxide in cellular systems

    International Nuclear Information System (INIS)

    Low concentrations (less than 1%) of both DMSO and glycerol gave surprisingly good cryoprotection to mammalian cells in tissue culture. Dextrans of varying molecular weight were cryoprotective. Combinations of these dextrans with small and essentially undamaging (in an osmotic sense) amounts of either DMSO or glycerol resulted in additive cryoprotection, and in some instances in synergism. Glycerol was as effective as DMSO in these combinations. If combinations of clinically acceptable dextrans (Rheomacrodex) and glycerol provide as much protection against freezing damage to other cell and tissue systems as they do to Chinese hamster cells in tissue culture, as demonstrated in this paper, then it should be possible to preserve tested or transplanted bone marrow, lymphocytes, platelets, and other cells without either the osmotic damage associated with high concentrations of glycerol or the toxicity problems associated with the cryoprotectant

  19. Efficient Uplink Modeling for Dynamic System-Level Simulations of Cellular and Mobile Networks

    Directory of Open Access Journals (Sweden)

    Lobinger Andreas

    2010-01-01

    Full Text Available A novel theoretical framework for uplink simulations is proposed. It allows investigations which have to cover a very long (real- time and which at the same time require a certain level of accuracy in terms of radio resource management, quality of service, and mobility. This is of particular importance for simulations of self-organizing networks. For this purpose, conventional system level simulators are not suitable due to slow simulation speeds far beyond real-time. Simpler, snapshot-based tools are lacking the aforementioned accuracy. The runtime improvements are achieved by deriving abstract theoretical models for the MAC layer behavior. The focus in this work is long term evolution, and the most important uplink effects such as fluctuating interference, power control, power limitation, adaptive transmission bandwidth, and control channel limitations are considered. Limitations of the abstract models will be discussed as well. Exemplary results are given at the end to demonstrate the capability of the derived framework.

  20. SYSTEM LEVEL SIMULATION FOR TWO TIER MACRO-FEMTO CELLULAR NETWORKS

    Directory of Open Access Journals (Sweden)

    Shiqi Xing

    2014-12-01

    Full Text Available LTE is an emerging wireless communication technology to provide high- speed data service for the mobile phones and data terminals. To improve indoor coverage and capacity Femtocells are included in 3GPP since Release 8. There is no common simulation platform is available for performance justification of LTEFemtocells. LTE-Sim is an object-oriented open source simulator which incorporates a complete protocol stack can be used for simulating two-tier macro-femto scenarios. To the best of our knowledge no paper provides the guideline to perform system level simulation of Femtocell networks. Here, in this paper Femtocells performance is evaluated in multi-Macrocells and multi-Femtocells environment with interference from Microcells and Macrocell users along with the scripting

  1. Joint Beam-Power Coordinative Scheduling of Neighboring Sectors in Cellular Systems

    Directory of Open Access Journals (Sweden)

    Fei Ji

    2011-12-01

    Full Text Available Beam cooperative scheduling of downlink transmission is an important technique to improve the spectrum efficiency in next generation mobile networks. This paper focuses on switched beams (the emission angles of the beams are fixed and proposes a joint beam-power coordinative scheduling algorithm among neighbor sectors in the downlink of mobile systems. Each sector coordinates the applied order and transmitted power of the beams with adjacent interfering sector, so as to reduce inter-sector interference and maximize throughputs. This scheduling problem is modeled as a constrained optimization problem and solved by our proposed iterative approach. Computer simulation shows that the proposed approach significantly outperform the existing round robin beam servicing approach and the approach that applies only beam cooperative scheduling.

  2. Cellular transcripts regulated during infections with Highly Pathogenic H5N1 Avian Influenza virus in 3 host systems

    Directory of Open Access Journals (Sweden)

    Noor Suriani M

    2011-04-01

    Full Text Available Abstract Background Highly pathogenic Avian Influenza (HPAI virus is able to infect many hosts and the virus replicates in high levels in the respiratory tract inducing severe lung lesions. The pathogenesis of the disease is actually the outcome of the infection as determined by complex host-virus interactions involving the functional kinetics of large numbers of participating genes. Understanding the genes and proteins involved in host cellular responses are therefore, critical for the elucidation of the mechanisms of infection. Methods Differentially expressed transcripts regulated in a H5N1 infections of whole lung organ of chicken, in-vitro chick embryo lung primary cell culture (CeLu and a continuous Madin Darby Canine Kidney cell line was undertaken. An improved mRNA differential display technique (Gene Fishing™ using annealing control primers that generates reproducible, authentic and long PCR products that are detectable on agarose gels was used for the identification of differentially expressed genes (DEGs. Seven of the genes have been selected for validation using a TaqMan® based real time quantitative PCR assay. Results Thirty seven known and unique differentially expressed genes from lungs of chickens, CeLu and MDCK cells were isolated. Among the genes isolated and identified include heat shock proteins, Cyclin D2, Prenyl (decaprenyl diphosphate synthase, IL-8 and many other unknown genes. The quantitative real time RT-PCR assay data showed that the transcription kinetics of the selected genes were clearly altered during infection by the Highly Pathogenic Avian Influenza virus. Conclusion The Gene Fishing™ technique has allowed for the first time, the isolation and identification of sequences of host cellular genes regulated during H5N1 virus infection. In this limited study, the differentially expressed genes in the three host systems were not identical, thus suggesting that their responses to the H5N1 infection may not share

  3. Identification of multiple cellular uptake pathways of polystyrene nanoparticles and factors affecting the uptake: relevance for drug delivery systems.

    Science.gov (United States)

    Firdessa, Rebuma; Oelschlaeger, Tobias A; Moll, Heidrun

    2014-01-01

    Nanoparticles may address challenges by human diseases through improving diagnosis, vaccination and treatment. The uptake mechanism regulates the type of threat a particle poses on the host cells and how a cell responds to it. Hence, understanding the uptake mechanisms and cellular interactions of nanoparticles at the cellular and subcellular level is a prerequisite for their effective biomedical applications. The present study shows the uptake mechanisms of polystyrene nanoparticles and factors affecting their uptake in bone marrow-derived macrophages, 293T kidney epithelial cells and L929 fibroblasts. Labeling with the endocytic marker FM4-64 and transmission electron microscopy studies show that the nanoparticles were internalized rapidly via endocytosis and accumulated in intracellular vesicles. Soon after their internalizations, nanoparticles trafficked to organelles with acidic pH. Analysis of the ultrastructural morphology of the plasma membrane invaginations or extravasations provides clear evidence for the involvement of several uptake routes in parallel to internalize a given type of nanoparticles by mammalian cells, highlighting the complexity of the nanoparticle-cell interactions. Blocking the specific endocytic pathways by different pharmacological inhibitors shows similar outcomes. The potential to take up nanoparticles varies highly among different cell types in a particle sizes-, time- and energy-dependent manner. Furthermore, infection and the activation status of bone marrow-derived macrophages significantly affect the uptake potential of the cells, indicating the need to understand the diseases' pathogenesis to establish effective and rational drug-delivery systems. This study enhances our understanding of the application of nanotechnology in biomedical sciences. PMID:25224362

  4. Activation of Hepatic STAT3 Maintains Pulmonary Defense during Endotoxemia.

    Science.gov (United States)

    Hilliard, Kristie L; Allen, Eri; Traber, Katrina E; Kim, Yuri; Wasserman, Gregory A; Jones, Matthew R; Mizgerd, Joseph P; Quinton, Lee J

    2015-10-01

    Pneumonia and infection-induced sepsis are worldwide public health concerns. Both pathologies elicit systemic inflammation and induce a robust acute-phase response (APR). Although APR activation is well regarded as a hallmark of infection, the direct contributions of liver activation to pulmonary defense during sepsis remain unclear. By targeting STAT3-dependent acute-phase changes in the liver, we evaluated the role of liver STAT3 activity in promoting host defense in the context of sepsis and pneumonia. We employed a two-hit endotoxemia/pneumonia model, whereby administration of 18 h of intraperitoneal lipopolysaccharide (LPS; 5 mg/kg of body weight) was followed by intratracheal Escherichia coli (10(6) CFU) in wild-type mice or those lacking hepatocyte STAT3 (hepSTAT3(-/-)). Pneumonia alone (without endotoxemia) was effectively controlled in the absence of liver STAT3. Following endotoxemia and pneumonia, however, hepSTAT3(-/-) mice, with significantly reduced levels of circulating and airspace acute-phase proteins, exhibited significantly elevated lung and blood bacterial burdens and mortality. These data suggested that STAT3-dependent liver responses are necessary to promote host defense. While neither recruited airspace neutrophils nor lung injury was altered in endotoxemic hepSTAT3(-/-) mice, alveolar macrophage reactive oxygen species generation was significantly decreased. Additionally, bronchoalveolar lavage fluid from this group of hepSTAT3(-/-) mice allowed greater bacterial growth ex vivo. These results suggest that hepatic STAT3 activation promotes both cellular and humoral lung defenses. Taken together, induction of liver STAT3-dependent gene expression programs is essential to countering the deleterious consequences of sepsis on pneumonia susceptibility. PMID:26216424

  5. Integrated cyber defenses towards cyber defense doctrine

    OpenAIRE

    Cloud, Donald W.

    2007-01-01

    At the same time that the Department of Defense (DoD) has leveraged Network Centric Warfare concepts to increase the operational effectiveness of U.S. military forces and to gain decision superiority over adversaries, the DoD has become increasingly dependent upon the secure operations of computer networks and infrastructure. As a result, DoD computer network operations have become a vital center of gravity of U.S. military forces. Unfortunately, computer networks are growing faster than ...

  6. Unfolding Green Defense

    DEFF Research Database (Denmark)

    Larsen, Kristian Knus

    2015-01-01

    consumption in military operations, defense expenditure, energy security, and global climate change. The report then proceeds to introduce the NATO Green Defence Framework before exploring specific current uses of green technologies and green strategies for defense. The report concludes that a number of...... political, military, organizational, and technological challenges and possibilities are related to the development of green solutions for defense. Based on this conclusion the report argues that it is essential to comprehensively describe how a green solution is linked to a security challenge to develop...

  7. Immunology defense system in insects: an approach in velvetbean catterpillar, Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae, AgMNPV-resistant/ Sistema imunológico de defesa em insetos: uma abordagem em lagartas da soja, Anticarsia gemmatalis Hübner (Lepidoptera: Noctuidae, resistentes ao AgMNPV

    Directory of Open Access Journals (Sweden)

    Ângela Maria Ferreira Falleiros

    2004-05-01

    Full Text Available The insects occupy almost all ecological niches and are constantly exposed to the attack of innumerable natural enemies, many of which are potentially pathogenic. To survive to these attacks, the insects had developed efficient defense systems, represented by outer cuticle, the digestory system and hemolymph cells, the hemocytes, that promote cellular defense. The velvetbean caterpillar, Anticarsia gemmatalis, as soybean pest crop, demand 60% of the insecticide applications in the national soybean crops. With the more and more use of the nucleopolihedrovirus Baculovirus anticarsia aiming at its control, studies involving the foreing body recognition and its destruction by the defense systems together with the appearance of resistant strains caterpillar are supports for methodologies that become the control of this more efficient, until as prevention for the appearing of caterpillar naturally resistant in the field.Os insetos ocupam quase todos os nichos ecológicos e estão constantemente expostos ao ataque de inúmeros inimigos naturais, muitos dos quais são potencialmente patogênicos. Para sobreviver a esses ataques, os insetos desenvolveram eficiente sistema de defesa, representado pela espessa cutícula que os recobre, pelo sistema digestório e por células da hemolinfa, os hemócitos, que promovem a defesa celular. A lagarta da soja, Anticarsia gemmatalis, como praga desta cultura, demanda 60% das aplicações de inseticidas nas lavouras de soja nacionais. Com a utilização cada vez mais ampla do nucleopoliedrovirus Baculovirus anticarsia visando seu controle, estudos envolvendo o reconhecimento de corpos estranhos pelo sistema de defesa e sua destruição juntamente com o aparecimento de linhagens de lagartas resistentes, são suportes para metodologias que tornam o controle deste inseto-praga mais eficaz, até como prevenção para o surgimento de lagartas naturalmente resistentes no campo.

  8. Cellular-signaling pathways unveil the carcinogenic potential of chemicals.

    Science.gov (United States)

    Hendriks, Giel; van de Water, Bob; Schoonen, Willem; Vrieling, Harry

    2013-06-01

    Most of the current in vitro carcinogenicity assays assess the potential carcinogenic properties of chemicals through the detection of inflicted DNA damage or subsequent chromosome damage and gene mutations. Unfortunately, these assays generally do not provide mechanistic insight into the reactive properties of a chemical. Upon chemical-induced damage of biomolecules, molecular sensors will activate general and damage-specific cellular response pathways that provide protection against the (geno)toxic and potential carcinogenic properties of chemicals. These cellular defense mechanisms include activation of cell-cycle checkpoints, DNA repair systems and induction of apoptosis or necrosis. Visualization of activated cellular-signaling pathways forms a powerful means to readily detect the genotoxic potential of chemical compounds and simultaneously gain insight into their reactive properties. Over the past years, various in vitro reporter assays have been developed that monitor activation of general and more specific cellular-signaling pathways, including the GreenScreen HC and ToxTracker assays. In this review we provide a perspective on how we can exploit activation of cellular signaling pathways to shed light on the mode of action of the chemical exposure and to develop sophisticated mechanism-based in vitro assays for cancer risk assessment. PMID:23339022

  9. Department of Defense Worldwide Numbers for TBI

    Science.gov (United States)

    ... the Defense Medical Surveillance System , a continuously expanding relational database that documents military and medical experiences of ... Section 508 FAQs Technical Issues & Maintenance Schedule External Links Disclaimer No Fear Act USA.gov This site's ...

  10. Defense Meteorological Satellite Program (DMSP) Film

    Data.gov (United States)

    National Oceanic and Atmospheric Administration, Department of Commerce — The United States Air Force Defense Meteorological Satellite Program (DMSP) Operational Linescan System (OLS) is a polar orbiting meteorological sensor with two...

  11. Perilipin Expression Reveals Adipogenic Potential of hADSCs inside Superporous Polymeric Cellular Delivery Systems

    Directory of Open Access Journals (Sweden)

    Sorina Dinescu

    2014-01-01

    Full Text Available Recent progress in tissue engineering and regenerative medicine envisages the use of cell-scaffold bioconstructs to best mimic the natural in vivo microenvironment. Our aim was not only to develop novel 3D porous scaffolds for regenerative applications by the association of gelatin (G, alginate (A, and polyacrylamide (PAA major assets but also to evaluate their in vitro potential to support human adipose-derived stem cells (hADSCs adipogenesis. G-A-PAA biomatrix investigated in this work is an interesting substrate combining the advantages of the three individual constituents, namely, biodegradability of G, hydrophilicity of A and PAA, superior elasticity at compression with respect to the G-A and PAA controls, and the capacity to generate porous scaffolds. hADSCs inside these novel interpenetrating polymer networks (IPNs were able to populate the entire scaffold structure and to display their characteristic spindle-like shape as a consequence of a good interaction with G component of the matrices. Additionally, hADSCs proved to display the capacity to differentiate towards mature adipocytes, to accumulate lipids inside their cytoplasm, and to express perilipin late adipogenic marker inside novel IPNs described in this study. On long term, this newly designed biomatrix aims to represent a stem cell delivery system product dedicated for modern regenerative strategies.

  12. Carp erythrodermatitis: host defense-pathogen interaction.

    OpenAIRE

    Pourreau, C.N.

    1990-01-01

    The outcome of a bacterial infection depends on the interaction between pathogen and host. The ability of the microbe to survive in the host depends on its invasive potential (i.e. spreading and multiplication), and its ability to obtain essential nutrients and to resist the host's defense system. On the other hand, the host's resistance to a bacterial attack depends on its physiological state, the intensity of the bacterial attack and the efficacy of the defense system to neutralize toxins a...

  13. Auxins in defense strategies

    OpenAIRE

    Čarná, M. (Mária); Repka, V.; Skůpa, P. (Petr); Šturdík, E.

    2014-01-01

    Plant hormones operate in a very complex network where they regulate and control different vital mechanisms. They coordinate growth, development and defense via signaling involving different interactions of molecules. Activation of molecules responsible for regulation of plant immunity is mainly provided by salicylic and jasmonic acid signaling pathways. Similar to the signaling of these defense-associated plant hormones, auxin can also affect resistance to different pathogen groups and disea...

  14. Surfing China's National Defense

    Institute of Scientific and Technical Information of China (English)

    Ji Guilin

    2010-01-01

    @@ Following the start of its first test run on August 20, 2009, the website www.mod.gov.cn of the Ministry of National Defense (MOD) of the People's Republic of China has logged more than 2 billion hits,from many countries and regions including China, the United States,the United Kingdom, Japan, Australia and Singapore. China National Defense News reporters recently interviewed Ji Guilin, the website's Editor in Chief, on its performance and the feedback of netizens.

  15. Understanding Defensive Equity

    OpenAIRE

    Robert Novy-Marx

    2014-01-01

    High volatility and high beta stocks tilt strongly to small, unprofitable, and growth firms. These tilts explain the poor absolute performance of the most aggressive stocks. In conjunction with the well documented inability of the Fama and French three-factor model to price small growth stocks, especially unprofitable small growth stocks, these tilts also drive the abnormal performance of defensive equity (i.e., low volatility and/or low beta strategies). While defensive strategy performance ...

  16. Remodelling of cellular excitation (reaction) and intercellular coupling (diffusion) by chronic atrial fibrillation represented by a reaction-diffusion system

    Science.gov (United States)

    Zhang, Henggui; Garratt, Clifford J.; Kharche, Sanjay; Holden, Arun V.

    2009-06-01

    Human atrial tissue is an excitable system, in which myocytes are excitable elements, and cell-to-cell electrotonic interactions are via diffusive interactions of cell membrane potentials. We developed a family of excitable system models for human atrium at cellular, tissue and anatomical levels for both normal and chronic atrial fibrillation (AF) conditions. The effects of AF-induced remodelling of cell membrane ionic channels (reaction kinetics) and intercellular gap junctional coupling (diffusion) on atrial excitability, conduction of excitation waves and dynamics of re-entrant excitation waves are quantified. Both ionic channel and gap junctional coupling remodelling have rate dependent effects on atrial propagation. Membrane channel conductance remodelling allows the propagation of activity at higher rates than those sustained in normal tissue or in tissue with gap junctional remodelling alone. Membrane channel conductance remodelling is essential for the propagation of activity at rates higher than 300/min as seen in AF. Spatially heterogeneous gap junction coupling remodelling increased the risk of conduction block, an essential factor for the genesis of re-entry. In 2D and 3D anatomical models, the dynamical behaviours of re-entrant excitation waves are also altered by membrane channel modelling. This study provides insights to understand the pro-arrhythmic effects of AF-induced reaction and diffusion remodelling in atrial tissue.

  17. Transforming the force : a comparative analysis of the Department of Defense's (DoD'S) Enterprise Resource Planning (ERP) systems

    OpenAIRE

    Hill, Charles W.

    2007-01-01

    Automated Information Systems (AIS) are Software systems that support administrative functions, such as accounting, payroll, finance, personnel, inventory control, logistics, and equipment and maintenance scheduling. An ERP system is a type of AIS that works to integrate all the different functional business areas of an organization. Since the 1990s, a large number of corporations have transitioned from legacy proprietary software to an ERP. The companies who have successfully made the transi...

  18. Nutrient supply and simulated herbivory differentially influence the content and the potential effectiveness of glucosinolate-based defense system in brassica species

    Science.gov (United States)

    • Environmental stresses often result in upregulation of defense metabolism in plants. However, the dynamics of nitrogen-containing defense compounds that could be remobilized for maintenance-metabolism is less predictable under stress. Even less known is the impact of nutrient deficiency on the eff...

  19. Evaluating and managing Cold War era historic properties : the cultural significance of U.S. Air Force defensive radar systems.

    Energy Technology Data Exchange (ETDEWEB)

    Whorton, M.

    1999-01-20

    Aircraft and later missile radar early warning stations played an important role in the Cold War. They are associated with important technological, social, political, and military themes of the Cold War and are worthy of preservation. The scope and scale of these systems make physical preservation impractical, but the U.S. Air Force program of historical evaluation and documentation of these systems will provide valuable information to future generations studying this historic period.

  20. 基于DoDAF的舰载弹炮结合防空武器系统模型%Shipboard Missile Gun Integrated Air-Defense Weapon System Model Based on DoDAF

    Institute of Scientific and Technical Information of China (English)

    王洪胜; 禹大勇; 曲延明

    2014-01-01

    In current sea battle, aiming at the point-defense ship-to-air missile and small caliber shipboard artillery can’t realize reserving battle task independently, establish shipboard missile-gun integrated air-defense weapon system based on DoDAF. Analyze design process of shipboard missile-gun integrated air-defense weapon system, combining with military requirement and battle flow to establish part of battle model, then describe operation process of shipboard missile-gun integrated air-defense weapon system in water surface ship terminal defense. It promotes the top layer design of shipboard missile-gun integrated air-defense weapon system and gives technology support for danger control.%针对现代海战中点防御舰空导弹和小口径舰炮难以独立完成预定作战任务的问题,构建一种基于DoDAF的舰载弹炮结合防空武器系统模型。分析了舰载弹炮结合防空武器系统设计步骤,结合军事需求、作战流程构建了部分作战模型,并阐述了舰载弹炮结合防空武器系统在水面舰艇末端防御作战中的活动过程。目的是推动舰载弹炮结合防空武器系统顶层设计和风险控制提供技术支撑。