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Sample records for cells including detailed

  1. Method for including detailed evaluation of daylight levels in Be06

    DEFF Research Database (Denmark)

    Petersen, Steffen

    2008-01-01

    . In order to evaluate whether a certain design is good daylight design or not building designers must perform detailed evaluation of daylight levels, including the daylight performance of dynamic solar shadings, and include these in the energy performance evaluation. However, the mandatory national...... for electrical lighting by 30% compared to the method suggested in this paper, which is based on hourly calculated illuminance levels in a representative point in the workspace. Implementing the suggested method in Be06 would be a better representation of the reality compared to the current method. Furthermore...

  2. Including excitons in semiconductor solar cell modelling

    OpenAIRE

    Burgelman, Marc; Minnaert, Ben

    2005-01-01

    Excitons are marginally important in classical semiconductor device physics, and their treatment is not included in standard solar cell modelling. However, in organic semiconductors and solar cells, the role of excitons is essential, as the primary effect of light absorption is exciton generation, and free electrons and holes are created by exciton dissociation. First steps to include excitons in solar cell modelling were presented by Green 1996 and Zhang 1998. Their model was restricted to a...

  3. Can quantum coherent solar cells break detailed balance?

    Energy Technology Data Exchange (ETDEWEB)

    Kirk, Alexander P. [School of Electrical, Computer and Energy Engineering, Arizona State University, Tempe, Arizona 85287 (United States)

    2015-07-21

    Carefully engineered coherent quantum states have been proposed as a design attribute that is hypothesized to enable solar photovoltaic cells to break the detailed balance (or radiative) limit of power conversion efficiency by possibly causing radiative recombination to be suppressed. However, in full compliance with the principles of statistical mechanics and the laws of thermodynamics, specially prepared coherent quantum states do not allow a solar photovoltaic cell—a quantum threshold energy conversion device—to exceed the detailed balance limit of power conversion efficiency. At the condition given by steady-state open circuit operation with zero nonradiative recombination, the photon absorption rate (or carrier photogeneration rate) must balance the photon emission rate (or carrier radiative recombination rate) thus ensuring that detailed balance prevails. Quantum state transitions, entropy-generating hot carrier relaxation, and photon absorption and emission rate balancing are employed holistically and self-consistently along with calculations of current density, voltage, and power conversion efficiency to explain why detailed balance may not be violated in solar photovoltaic cells.

  4. Can quantum coherent solar cells break detailed balance?

    International Nuclear Information System (INIS)

    Carefully engineered coherent quantum states have been proposed as a design attribute that is hypothesized to enable solar photovoltaic cells to break the detailed balance (or radiative) limit of power conversion efficiency by possibly causing radiative recombination to be suppressed. However, in full compliance with the principles of statistical mechanics and the laws of thermodynamics, specially prepared coherent quantum states do not allow a solar photovoltaic cell—a quantum threshold energy conversion device—to exceed the detailed balance limit of power conversion efficiency. At the condition given by steady-state open circuit operation with zero nonradiative recombination, the photon absorption rate (or carrier photogeneration rate) must balance the photon emission rate (or carrier radiative recombination rate) thus ensuring that detailed balance prevails. Quantum state transitions, entropy-generating hot carrier relaxation, and photon absorption and emission rate balancing are employed holistically and self-consistently along with calculations of current density, voltage, and power conversion efficiency to explain why detailed balance may not be violated in solar photovoltaic cells

  5. Detailed balance limit efficiency of silicon intermediate band solar cells

    Institute of Scientific and Technical Information of China (English)

    Cao Quan; Ma Zhi-Hua; Xue Chun-Lai; Zuo Yu-Hua; Wang Qi-Ming

    2011-01-01

    The detailed balance method is used to study the potential of the intermediate band solar cell (IBSC),which can improve the efficiency of the Si-based solar cell with a bandgap between 1.1 eV to 1.7 eV. It shows that a crystalline silicon solar cell with an intermediate band located at 0.36 eV below the conduction band or above the valence band can reach a limiting efficiency of 54% at the maximum light concentration,improving greatly than 40.7% of the Shockley-Queisser limit for the single junction Si solar cell. The simulation also shows that the limiting efficiency of the siliconbased solar cell increases as the bandgap increases from 1.1 eV to 1.7 eV,and the amorphous Si solar cell with a bandgap of 1.7 eV exhibits a radiative limiting efficiency of 62.47%,having a better potential.

  6. Galvanic cells including cobalt-chromium alloys.

    Science.gov (United States)

    Gjerdet, N R

    1980-01-01

    Galvanic cells may be created when dentures made of cobalt-chromium alloys are placed on teeth with metallic restorations. The power of such cells was evaluated in an in vitro galvanic using amalgams, gold alloy, and nickel-chromium alloys. The amalgams and one of the nickel-chromium alloys revealed high corrosion currents when placed in contact with cobalt-chromium alloy, the conventional amalgam showing the highest values. The gold alloy and another nickel-chromium alloy exhibited low corrosion currents and they were noble with respect to cobalt-chromium.

  7. Tomographic brain imaging with nucleolar detail and automatic cell counting

    Science.gov (United States)

    Hieber, Simone E.; Bikis, Christos; Khimchenko, Anna; Schweighauser, Gabriel; Hench, Jürgen; Chicherova, Natalia; Schulz, Georg; Müller, Bert

    2016-09-01

    Brain tissue evaluation is essential for gaining in-depth insight into its diseases and disorders. Imaging the human brain in three dimensions has always been a challenge on the cell level. In vivo methods lack spatial resolution, and optical microscopy has a limited penetration depth. Herein, we show that hard X-ray phase tomography can visualise a volume of up to 43 mm3 of human post mortem or biopsy brain samples, by demonstrating the method on the cerebellum. We automatically identified 5,000 Purkinje cells with an error of less than 5% at their layer and determined the local surface density to 165 cells per mm2 on average. Moreover, we highlight that three-dimensional data allows for the segmentation of sub-cellular structures, including dendritic tree and Purkinje cell nucleoli, without dedicated staining. The method suggests that automatic cell feature quantification of human tissues is feasible in phase tomograms obtained with isotropic resolution in a label-free manner.

  8. Tomographic brain imaging with nucleolar detail and automatic cell counting.

    Science.gov (United States)

    Hieber, Simone E; Bikis, Christos; Khimchenko, Anna; Schweighauser, Gabriel; Hench, Jürgen; Chicherova, Natalia; Schulz, Georg; Müller, Bert

    2016-01-01

    Brain tissue evaluation is essential for gaining in-depth insight into its diseases and disorders. Imaging the human brain in three dimensions has always been a challenge on the cell level. In vivo methods lack spatial resolution, and optical microscopy has a limited penetration depth. Herein, we show that hard X-ray phase tomography can visualise a volume of up to 43 mm(3) of human post mortem or biopsy brain samples, by demonstrating the method on the cerebellum. We automatically identified 5,000 Purkinje cells with an error of less than 5% at their layer and determined the local surface density to 165 cells per mm(2) on average. Moreover, we highlight that three-dimensional data allows for the segmentation of sub-cellular structures, including dendritic tree and Purkinje cell nucleoli, without dedicated staining. The method suggests that automatic cell feature quantification of human tissues is feasible in phase tomograms obtained with isotropic resolution in a label-free manner. PMID:27581254

  9. Treatment Options for Plasma Cell Neoplasms (Including Multiple Myeloma)

    Science.gov (United States)

    ... Treatment Health Professional Plasma Cell Neoplasms Treatment Research Plasma Cell Neoplasms (Including Multiple Myeloma) Treatment (PDQ®)–Patient Version General Information About Plasma Cell Neoplasms Go to Health Professional Version Key ...

  10. Stages of Plasma Cell Neoplasms (Including Multiple Myeloma)

    Science.gov (United States)

    ... Treatment Health Professional Plasma Cell Neoplasms Treatment Research Plasma Cell Neoplasms (Including Multiple Myeloma) Treatment (PDQ®)–Patient Version General Information About Plasma Cell Neoplasms Go to Health Professional Version Key ...

  11. Detailed Functional and Proteomic Characterization of Fludarabine Resistance in Mantle Cell Lymphoma Cells.

    Directory of Open Access Journals (Sweden)

    Lucie Lorkova

    Full Text Available Mantle cell lymphoma (MCL is a chronically relapsing aggressive type of B-cell non-Hodgkin lymphoma considered incurable by currently used treatment approaches. Fludarabine is a purine analog clinically still widely used in the therapy of relapsed MCL. Molecular mechanisms of fludarabine resistance have not, however, been studied in the setting of MCL so far. We therefore derived fludarabine-resistant MCL cells (Mino/FR and performed their detailed functional and proteomic characterization compared to the original fludarabine sensitive cells (Mino. We demonstrated that Mino/FR were highly cross-resistant to other antinucleosides (cytarabine, cladribine, gemcitabine and to an inhibitor of Bruton tyrosine kinase (BTK ibrutinib. Sensitivity to other types of anti-lymphoma agents was altered only mildly (methotrexate, doxorubicin, bortezomib or remained unaffacted (cisplatin, bendamustine. The detailed proteomic analysis of Mino/FR compared to Mino cells unveiled over 300 differentially expressed proteins. Mino/FR were characterized by the marked downregulation of deoxycytidine kinase (dCK and BTK (thus explaining the observed crossresistance to antinucleosides and ibrutinib, but also by the upregulation of several enzymes of de novo nucleotide synthesis, as well as the up-regulation of the numerous proteins of DNA repair and replication. The significant upregulation of the key antiapoptotic protein Bcl-2 in Mino/FR cells was associated with the markedly increased sensitivity of the fludarabine-resistant MCL cells to Bcl-2-specific inhibitor ABT199 compared to fludarabine-sensitive cells. Our data thus demonstrate that a detailed molecular analysis of drug-resistant tumor cells can indeed open a way to personalized therapy of resistant malignancies.

  12. Multimillion-cell SAGD models, opportunity for detailed field analysis

    Energy Technology Data Exchange (ETDEWEB)

    Akram, F. [Schlumberger Canada Limited (Canada)

    2011-07-01

    In the heavy oil industry, steam assisted gravity drainage (SAGD) is a new thermal oil recovery process used to enhance oil recovery. However, operators have trouble reaching their objectives. A better understanding of reservoir response is necessary to reach the desired production rates. The aim of this paper is to present a new methodology for carrying out a reservoir simulation study. A multimillion-cell model was developed with realistic properties, structural complexity and heterogeneities; both full field and thermal simulations were conducted. Results showed that the computing technology thus developed offers greater accuracy in less time than is required when using conventional techniques. In addition, this methodology provides a higher degree of confidence in the results by bridging the gap between geology and engineering. The paper is a first step in producing a framework and the methodology presented herein will need to be further tested to confirm its benefits over conventional techniques.

  13. Detailed NMR, Including 1,1-ADEQUATE, and Anticancer Studies of Compounds from the Echinoderm Colobometra perspinosa

    Directory of Open Access Journals (Sweden)

    Catherine H. Liptrot

    2009-11-01

    Full Text Available From the dichloromethane/methanol extract of the crinoid Colobometra perspinosa, collected south east of Richards Island (Bedara, Family Islands, Central Great Barrier Reef, Australia, 3-(1'-hydroxypropyl-1,6,8-trihydroxy-9,10-anthraquinone [one of the two stereoisomers of rhodoptilometrin, (1], 3-propyl-1,6,8-trihydroxy-9,10-anthraquinone (3, 2-[(phenylacetylamino]ethanesulfonic acid (4, and 4-hydroxybutanoic acid (5 were isolated. Comparison of 1H- and 13C-NMR data for rhodoptilometrin (1 with those reported in the literature showed significant differences for some resonances associated with rings A and C. In an attempt to provide accurately assigned 1H- and 13C-NMR data, as well as to confirm the structure of 1, a thorough NMR investigation of this compound was undertaken. Measurements included: concentration dependent 13C, 1D selective NOE, HSQC, HMBC and 1,1-ADEQUATE. The NMR data for 4 and 5 are reported here for the first time, as is their occurrence from the marine environment. The in vitro anticancer activity of the original extract was found to be associated with 1, 3 and 5.

  14. Fuel cell repeater unit including frame and separator plate

    Science.gov (United States)

    Yamanis, Jean; Hawkes, Justin R; Chiapetta, Jr., Louis; Bird, Connie E; Sun, Ellen Y; Croteau, Paul F

    2013-11-05

    An example fuel cell repeater includes a separator plate and a frame establishing at least a portion of a flow path that is operative to communicate fuel to or from at least one fuel cell held by the frame relative to the separator plate. The flow path has a perimeter and any fuel within the perimeter flow across the at least one fuel cell in a first direction. The separator plate, the frame, or both establish at least one conduit positioned outside the flow path perimeter. The conduit is outside of the flow path perimeter and is configured to direct flow in a second, different direction. The conduit is fluidly coupled with the flow path.

  15. Elliptical posts allow for detailed control of non-equibiaxial straining of cell cultures

    DEFF Research Database (Denmark)

    Olesen, Christian Gammelgaard; Pennisi, Cristian Pablo; de Zee, Mark;

    2013-01-01

    tissue cells in vivo are subjected to a range of mechanical deformations including shear strain caused by activities of daily living. Shear strains are suspected to play an important role in tissue necrosis. Method The Flexcell system was redesigned using a finite element model in order to obtain large...... areas of the membrane in a controlled, uniform non-equibiaxial strain state. Results The redesign was manufactured and the resulting strains were experimentally validated by means of image analysis methods. The results showed that the system could be used for experiments varying the shear strain....... Conclusion The result allows scientists and experimentalists to apply detailed control of the strain tensor applied to tissue samples in two dimensions....

  16. Molecular-detailed simulation of red blood cells in Stokes flows

    Science.gov (United States)

    Peng, Zhangli; Zhu, Qiang

    2010-11-01

    The red blood cell (RBC) membrane consists of a lipid bilayer and a cytoskeleton. By coupling a multiscale approach of RBC membranes with a boundary element method (BEM) for the exterior and interior fluids, we developed a numerical capacity to relate the fluid-structure interaction of RBCs in Stokes flows with detailed mechanical loads inside its molecular architecture. Our multiscale approach includes three models: in the whole cell level, a finite element method (FEM) is employed to model the lipid bilayer and the cytoskeleton as two distinct layers of continuum shells; the mechanical properties of the cytoskeleton are obtained from a molecular-based model; the spectrin, a major protein of the cytoskeleton, is simulated using a constitutive model. BEM is applied to predict the exterior and interior Stokes flows, and is coupled with the FEM of the membrane through a staggered coupling algorithm. Using this method, we simulated the tumbling and tank-treading behaviors of RBCs in shear flows, and investigated the RBC dynamics in capillary flows. The structural deformation of the cytoskeleton and the interaction force between the lipid bilayer and the cytoskeleton are predicted.

  17. Eukaryotic cell encystation and cancer cell dormancy: is a greater devil veiled in the details of a lesser evil?

    OpenAIRE

    Baig, Abdul Mannan; Khan, Naveed Ahmed; Abbas, Farhat

    2015-01-01

    Cancer cell dormancy is the main cause of cancer recurrence and failure of therapy as dormant cells evade not only the anticancer drugs but also the host immune system. These dormant cells veil themselves from detection by imaging and/or using biomarkers, which imposes an additional problem in targeting such cells. A similar form of hibernation process known as encystation is studied in detail for pathogenic unicellular eukaryotic microorganisms. By examination using microarray gene expressio...

  18. A detailed approach to model transport, heterogeneous chemistry, and electrochemistry in solid-oxide fuel cells

    OpenAIRE

    Janardhanan, Vinod

    2007-01-01

    This book lays out a numerical framework for the detailed description of heterogeneous chemistry, electrochemistry and porous media transport in solid-oxide fuel cells (SOFC). Assuming hydrogen as the only electrochemically active species, a modified Butler-Volmer equation is used to model the electrochemical charge transfer.

  19. Modelling of Dual-Junction Solar Cells including Tunnel Junction

    OpenAIRE

    Abdelaziz Amine; Yamina Mir; Mimoun Zazoui

    2013-01-01

    Monolithically stacked multijunction solar cells based on III–V semiconductors materials are the state-of-art of approach for high efficiency photovoltaic energy conversion, in particular for space applications. The individual subcells of the multi-junction structure are interconnected via tunnel diodes which must be optically transparent and connect the component cells with a minimum electrical resistance. The quality of these diodes determines the output performance of the solar cell. The p...

  20. Modelling of Dual-Junction Solar Cells including Tunnel Junction

    Directory of Open Access Journals (Sweden)

    Abdelaziz Amine

    2013-01-01

    Full Text Available Monolithically stacked multijunction solar cells based on III–V semiconductors materials are the state-of-art of approach for high efficiency photovoltaic energy conversion, in particular for space applications. The individual subcells of the multi-junction structure are interconnected via tunnel diodes which must be optically transparent and connect the component cells with a minimum electrical resistance. The quality of these diodes determines the output performance of the solar cell. The purpose of this work is to contribute to the investigation of the tunnel electrical resistance of such a multi-junction cell through the analysis of the current-voltage (J-V characteristics under illumination. Our approach is based on an equivalent circuit model of a diode for each subcell. We examine the effect of tunnel resistance on the performance of a multi-junction cell using minimization of the least squares technique.

  1. Flexible organic solar cells including efficiency enhancing grating structures

    DEFF Research Database (Denmark)

    Oliveira Hansen, Roana Melina de; Liu, Yinghui; Madsen, Morten;

    2013-01-01

    In this work, a new method for the fabrication of organic solar cells containing functional light-trapping nanostructures on flexible substrates is presented. Polyimide is spin-coated on silicon support substrates, enabling standard micro- and nanotechnology fabrication techniques......, such as photolithography and electron-beam lithography, besides the steps required for the bulk-heterojunction organic solar cell fabrication. After the production steps, the solar cells on polyimide are peeled off the silicon support substrates, resulting in flexible devices containing nanostructures for light absorption...... enhancement. Since the solar cells avoid using brittle electrodes, the performance of the flexible devices is not affected by the peeling process. We have investigated three different nanostructured grating designs and conclude that gratings with a 500 nm pitch distance have the highest light...

  2. Thymoquinone causes multiple effects, including cell death, on dividing plant cells.

    Science.gov (United States)

    Hassanien, Sameh E; Ramadan, Ahmed M; Azeiz, Ahmed Z Abdel; Mohammed, Rasha A; Hassan, Sabah M; Shokry, Ahmed M; Atef, Ahmed; Kamal, Khalid B H; Rabah, Samar; Sabir, Jamal S M; Abuzinadah, Osama A; El-Domyati, Fotouh M; Martin, Gregory B; Bahieldin, Ahmed

    2013-01-01

    Thymoquinone (TQ) is a major constituent of Nigella sativa oil with reported anti-oxidative activity and anti-inflammatory activity in animal cells. It also inhibits proliferation and induces programmed cell death (apoptosis) in human skin cancer cells. The present study sought to detect the influence of TQ on dividing cells of three plant systems and on expression of Bcl2-associated athanogene-like (BAG-like) genes that might be involved during the process of cell death. BAG genes are known for the regulation of diverse physiological processes in animals, including apoptosis, tumorigenesis, stress responses, and cell division. Synthetic TQ at 0.1mg/mL greatly reduced wheat seed germination rate, whereas 0.2mg/mL completely inhibited germination. An Evans blue assay revealed moderate cell death in the meristematic zone of Glycine max roots after 1h of TQ treatment (0.2mg/mL), with severe cell death occurring in this zone after 2h of treatment. Light microscopy of TQ-treated (0.2mg/mL) onion hairy root tips for 1h revealed anti-mitotic activity and also cell death-associated changes, including nuclear membrane disruption and nuclear fragmentation. Transmission electron microscopy of TQ-treated cells (0.2mg/mL) for 1h revealed shrinkage of the plasma membrane, leakage of cell lysate, degradation of cell walls, enlargement of vacuoles and condensation of nuclei. Expression of one BAG-like gene, previously associated with cell death, was induced 20 min after TQ treatment in Glycine max root tip cells. Thus, TQ has multiple effects, including cell death, on dividing plant cells and plants may serve as a useful system to further investigate the mechanisms underlying the response of eukaryotic cells to TQ. PMID:24296078

  3. Cell mechanics and stress: from molecular details to the 'universal cell reaction' and hormesis.

    Science.gov (United States)

    Agutter, Paul S

    2007-04-01

    The 'universal cell reaction' (UCR), a coordinated biphasic response to external (noxious and other) stimuli observed in all living cells, was described by Nasonov and his colleagues in the mid-20th century. This work has received no attention from cell biologists in the West, but the UCR merits serious consideration. Although it is non-specific, it is likely to be underpinned by precise mechanisms and, if these mechanisms were characterized and their relationship to the UCR elucidated, then our understanding of the integration of cellular function could be improved. As a step towards identifying such mechanisms, I review some recent advances in understanding cell mechanics and the stress response and I suggest potentially testable hypotheses. There is a particular need for time-course studies of cellular responses to different stimulus doses or intensities. I also suggest a correspondence with hormesis; re-investigation of the UCR using modern biophysical and molecular-biological techniques might throw light on this much-discussed phenomenon.

  4. Detailed dynamic Solid Oxide Fuel Cell modeling for electrochemical impedance spectra simulation

    Science.gov (United States)

    Hofmann, Ph.; Panopoulos, K. D.

    This paper presents a detailed flexible mathematical model for planar solid oxide fuel cells (SOFCs), which allows the simulation of steady-state performance characteristics, i.e. voltage-current density (V- j) curves, and dynamic operation behavior, with a special capability of simulating electrochemical impedance spectroscopy (EIS). The model is based on physico-chemical governing equations coupled with a detailed multi-component gas diffusion mechanism (Dusty-Gas Model (DGM)) and a multi-step heterogeneous reaction mechanism implicitly accounting for the water-gas-shift (WGS), methane reforming and Boudouard reactions. Spatial discretization can be applied for 1D (button-cell approximation) up to quasi-3D (full size anode supported cell in cross-flow configuration) geometries and is resolved with the finite difference method (FDM). The model is built and implemented on the commercially available modeling and simulations platform gPROMS™. Different fuels based on hydrogen, methane and syngas with inert diluents are run. The model is applied to demonstrate a detailed analysis of the SOFC inherent losses and their attribution to the EIS. This is achieved by means of a step-by-step analysis of the involved transient processes such as gas conversion in the main gas chambers/channels, gas diffusion through the porous electrodes together with the heterogeneous reactions on the nickel catalyst, and the double-layer current within the electrochemical reaction zone. The model is an important tool for analyzing SOFC performance fundamentals as well as for design and optimization of materials' and operational parameters.

  5. Detailed dynamic solid oxide fuel cell modeling for electrochemical impedance spectra simulation

    Energy Technology Data Exchange (ETDEWEB)

    Hofmann, Ph. [Laboratory of Steam Boilers and Thermal Plants, School of Mechanical Engineering, Thermal Engineering Section, National Technical University of Athens, Heroon Polytechniou 9, 15780 Athens (Greece); Panopoulos, K.D. [Institute for Solid Fuels Technology and Applications, Centre for Research and Technology Hellas, 4th km. Ptolemais-Mpodosakeio Hospital, Region of Kouri, P.O. Box 95, GR 502, 50200 Ptolemais (Greece)

    2010-08-15

    This paper presents a detailed flexible mathematical model for planar solid oxide fuel cells (SOFCs), which allows the simulation of steady-state performance characteristics, i.e. voltage-current density (V-j) curves, and dynamic operation behavior, with a special capability of simulating electrochemical impedance spectroscopy (EIS). The model is based on physico-chemical governing equations coupled with a detailed multi-component gas diffusion mechanism (Dusty-Gas Model (DGM)) and a multi-step heterogeneous reaction mechanism implicitly accounting for the water-gas-shift (WGS), methane reforming and Boudouard reactions. Spatial discretization can be applied for 1D (button-cell approximation) up to quasi-3D (full size anode supported cell in cross-flow configuration) geometries and is resolved with the finite difference method (FDM). The model is built and implemented on the commercially available modeling and simulations platform gPROMS trademark. Different fuels based on hydrogen, methane and syngas with inert diluents are run. The model is applied to demonstrate a detailed analysis of the SOFC inherent losses and their attribution to the EIS. This is achieved by means of a step-by-step analysis of the involved transient processes such as gas conversion in the main gas chambers/channels, gas diffusion through the porous electrodes together with the heterogeneous reactions on the nickel catalyst, and the double-layer current within the electrochemical reaction zone. The model is an important tool for analyzing SOFC performance fundamentals as well as for design and optimization of materials' and operational parameters. (author)

  6. Eukaryotic cell encystation and cancer cell dormancy: is a greater devil veiled in the details of a lesser evil?

    Science.gov (United States)

    Baig, Abdul Mannan; Khan, Naveed Ahmed; Abbas, Farhat

    2015-01-01

    Cancer cell dormancy is the main cause of cancer recurrence and failure of therapy as dormant cells evade not only the anticancer drugs but also the host immune system. These dormant cells veil themselves from detection by imaging and/or using biomarkers, which imposes an additional problem in targeting such cells. A similar form of hibernation process known as encystation is studied in detail for pathogenic unicellular eukaryotic microorganisms. By examination using microarray gene expression profiles, immunocytochemistry tools, and siRNAs during the process of encystation, understanding the covert features of cancer cell dormancy as proposed could be possible. This knowledge can be extended to dormant cancer cells to uncover the mechanisms that underlie this ghost, yet dangerous state of human cancers. We propose a strategy to induce dormancy and exit this state by application of knowledge gained from the encystation induction and retrieval processes in pathogenic eukaryotic microorganisms. Given that early detection and characterization of dormant malignant tumor cells is important as a general strategy to monitor and prevent the development of overt metastatic disease, this homology may enable the design of therapies that could either awake the dormant cell from dormancy to make it available for therapies or prolong such a phase to make cancer appear as a chronic disease. PMID:25859414

  7. Superficial Type of Multiple Basal Cell Carcinomas: Detailed Comparative Study of Its Dermoscopic and Histopathological Findings

    Directory of Open Access Journals (Sweden)

    Akiko Hirofuji

    2011-01-01

    Full Text Available We investigated in detail the dermoscopic and histopathological findings in a case of a superficial type of multiple basal cell carcinomas (BCCs. These multiple lesions (occurring in the chest, neck, and back showed three different findings, respectively. Dermoscopy of the erythematous and brown-colored patch on the anterior chest showed spoke wheel areas, and the histopathological cross-section revealed vertical spoke wheel structures. In the black- and brown-colored patch at the neck, the dermatoscopy showed a maple leaf-like structure, which was in accordance with the strengthening of the histological lateral connection of the lesion. The brown-colored patch of the lateral back histologically showed irregularly enlarged spoke wheel-like areas with peripheral increased melanin pigments, which correlated with the dark black color of dermoscopic maple leaf-like areas. The vertical spoke wheel areas by dermatoscopy revealed a horizontal spoke wheel structure by histopathology.

  8. New Two-Diode Model for Detailed Analysis of Multicrystalline Silicon Solar Cells

    Science.gov (United States)

    Kurobe, Ken-ichi; Matsunami, Hiroyuki

    2005-12-01

    In order to extract electrical properties of multicrystalline silicon (Mx-Si) solar cells precisely, current-voltage (I-V) measurement in the dark condition was carried out. A new two-diode model, which includes a diffusion-current dominant area (DCA) and a recombination-current dominant area (RCA), with nonequivalent series resistances, was proposed as a new equivalent circuit. Electrical properties as fitting parameters were successfully extracted using a successive approximation method. This characterization derived J01 and J02, which are the diode saturation current densities for n=1 and 2 diodes, respectively. To ensure this diode model, temperature characteristics were measured, and the validity of this model was shown through calculation of the energy band gap from J01. J01 and J02 indicate a power factor and a loss factor of solar cells, respectively, which are newly proposed to classify solar cell performance.

  9. 14 CFR Appendix D to Part 43 - Scope and Detail of Items (as Applicable to the Particular Aircraft) To Be Included in Annual and...

    Science.gov (United States)

    2010-01-01

    ...) Internal engine—for cylinder compression and for metal particles or foreign matter on screens and sump...—for improper installation and poor condition. (4) Antenna including trailing antenna—for...

  10. Detailed magnetic model simulations of the H- injection chicane magnets for the CERN PS Booster Upgrade, including eddy currents and influence on beam dynamics

    CERN Document Server

    Benedetto, E; Borburgh, J; Carli, C; Martini, M; Forte, V

    2014-01-01

    The CERN PS Booster will be upgraded with an H- injection system. The chicanemagnets for the injection bump ramp-down in 5 ms and generate eddy currents in the inconel vacuum chamber which perturb the homogeneity of the magnetic field. The multipolar field components are extracted from 3D OPERA simulations and are included in the lattice model. The -beating correction is computed all along the ramp and complete tracking simulations including space-charge are performed to evaluate the impact of these perturbations and correction on beam dynamics.

  11. Detailed uranium hydrogeochemical and stream sediment reconnaissance data release for the eastern portion of the Montrose NTMS quadrangle, Colorado, including concentrations of forty-five additional elements

    International Nuclear Information System (INIS)

    In September and October 1979, the Los Alamos Scientific Laboratory (LASL) conducted a detailed geochemical survey for uranium primarily in the Sawatch Range in the eastern part of the Montrose National Topographic Map Series (NTMS) quadrangle, Colorado, as part of the National Hydrogeochemical and Stream Sediment Reconnaissance (HSSR). This study was done as a follow-up to the uranium reconnaissance previously conducted by the LASL (Broxton et al, 1974). Totals of 1034 water and 2087 sediment samples were collected from streams and springs from 2088 locations within a 5420-km2 area. Statistical data for uranium concentrations in water and sediment samples are tabulated. Elemental concentration, field measurement, weather, geologic, and geographic data for each sample location are listed for waters in and for sediments in appendices. This report contains uranium analyses for water samples and multielement analyses for sediment samples. Sediments were analyzed for uranium and thorium as well as aluminum, antimony, arsenic, barium, beryllium, bismuth, cadmium, calcium, cerium, cesium, chlorine, chromium, cobalt, copper, dysprosium, europium, gold, hafnium, iron, lanthanum, lead, lithium, lutetium, magnesium, manganese, nickel, niobium, potassium, rubidium, samarium, scandium, selenium, silver, sodium, strontium, tantalum, terbium, tin, titanium, tungsten, vanadium, ytterbium, zinc, and zirconium. All elemental analyses were performed at the LASL. Water samples were analyzed for uranium by fluorometry and are reported as parts per billion. Sediments were analyzed for uranium by delayed neutron counting. Other elemental concentrations in sediments were determined by neutron activation analysis for 31 elements, by x-ray fluorescence for 12 elements, and by arc-source emission spectrography for 2 elements. Analytical results for sediments are reported as parts per million

  12. Detailed analysis of the clinical effects of cell therapy for thoracolumbar spinal cord injury: an original study

    Directory of Open Access Journals (Sweden)

    Sharma A

    2013-07-01

    Full Text Available Alok Sharma,1 Nandini Gokulchandran,1 Hemangi Sane,2 Prerna Badhe,1 Pooja Kulkarni,2 Mamta Lohia,3 Anjana Nagrajan,3 Nancy Thomas3 1Department of Medical Services and Clinical Research, 2Department of Research and Development, 3Department of Neurorehabilitation, NeuroGen Brain and Spine Institute, Surana Sethia Hospital and Research Centre, Chembur, Mumbai, India Background: Cell therapy is amongst the most promising treatment strategies in spinal cord injury (SCI because it focuses on repair. There are many published animal studies and a few human trials showing remarkable results with various cell types. The level of SCI determines whether paraplegia or quadriplegia is present, and greatly influences recovery. The purpose of this study was to determine the significance of the clinical effects and long-term safety of intrathecal administration of autologous bone marrow-derived mononuclear cells, along with changes in functional independence and quality of life in patients with thoracolumbar SCI. Methods: We undertook a retrospective analysis of a clinical study in which a nonrandomized sample of 110 patients with thoracolumbar SCI underwent autologous bone marrow-derived mononuclear cell transplantation intrathecally and subsequent neurorehabilitation, with a mean follow-up of 2 years ± 1 month. Changes on any parameters were recorded at follow-up. The data were analyzed using the Wilcoxon's signed-rank test and McNemar's test. Functional Independence Measure and American Spinal Injury Association (ASIA scores were recorded, and a detailed neurological assessment was performed. Results: Overall improvement was seen in 91% of patients, including reduction in spasticity, partial sensory recovery, and improvement in trunk control, postural hypotension, bladder management, mobility, activities of daily living, and functional independence. A significant association of these symptomatic improvements with the cell therapy intervention was established

  13. Physiology, morphology and detailed passive models of guinea-pig cerebellar Purkinje cells.

    Science.gov (United States)

    Rapp, M; Segev, I; Yarom, Y

    1994-01-01

    1. Purkinje cells (PCs) from guinea-pig cerebellar slices were physiologically characterized using intracellular techniques. Extracellular caesium ions were used to linearize the membrane properties of PCs near the resting potential. Under these conditions the average input resistance, RN, was 29 M omega, the average system time constant, tau 0, was 82 ms and the average cable length, LN, was 0.59. 2. Three PCs were fully reconstructed following physiological measurements and staining with horseradish peroxidase. Assuming that each spine has an area of 1 micron 2 and that the spine density over the spiny dendrites is ten spines per micrometre length, the total membrane area of each PC is approximately 150,000 microns 2, of which approximately 100,000 microns 2 is in the spines. 3. Detailed passive cable and compartmental models were built for each of the three reconstructed PCs. Computational methods were devised to incorporate globally the huge number of spines into these models. In all three cells the models predict that the specific membrane resistivity, Rm, of the soma is much lower than the dendritic Rm (approximately 500 and approximately 100,000 omega cm2 respectively). The specific membrane capacitance, Cm, is estimated to be 1.5-2 muF cm-2 and the specific cytoplasm resistivity, Ri, is 250 omega cm. 4. The average cable length of the dendrites according to the model is 0.13 lambda, suggesting that under caesium conditions PCs are electrically very compact. Brief somatic spikes, however, are expected to attenuate 30-fold when spreading passively into the dendritic terminals. A simulated 200 Hz train of fast, 90 mV somatic spikes produced a smooth 12 mV steady depolarization at the dendritic terminals. 5. A transient synaptic conductance increase, with a 1 nS peak at 0.5 ms and a driving force of 60 mV, is expected to produce approximately 20 mV peak depolarization at the spine head membrane. This EPSP then attenuates between 200- and 900-fold into the soma

  14. Circulating human B and plasma cells. Age-associated changes in counts and detailed characterization of circulating normal CD138(-) and CD138(+) plasma cells

    NARCIS (Netherlands)

    Caraux, Anouk; Klein, Bernard; Paiva, Bruno; Bret, Caroline; Schmitz, Alexander; Fuhler, Gwenny M.; Bos, Nico A.; Johnsen, Hans E.; Orfao, Alberto; Perez-Andres, Martin

    2010-01-01

    Generation of B and plasma cells involves several organs with a necessary cell trafficking between them. A detailed phenotypic characterization of four circulating B-cell subsets (immature-, naive-, memory- B-lymphocytes and plasma cells) of 106 healthy adults was realized by multiparametric flow cy

  15. Three dimensional model for particle saltation close to stream beds, including a detailed description of the particle interaction with turbulence and inter-particle collisions

    KAUST Repository

    Moreno, Pablo M.

    2011-05-19

    We present in this paper a new three-dimensional (3-D) model for bed-load sediment transport, based on a Lagrangian description. We analyze generalized sub-models for the velocities after collision and the representation of the bed-roughness. The free-flight sub-model includes the effect of several forces, such as buoyancy, drag, virtual mass, lift, Basset and Magnus, and also addresses the particle rotation. A recent methodology for saving computational time in the Basset force is also employed. The sub-models for the post-collision velocity and rotation are based on the conservation of linear and angular momentum during the collision with the bed. We develop a new 3-D representation for the bed roughness by using geometric considerations. In order to address the interaction of particles with the turbulent flow, we tracked the particles through a computed turbulent velocity field for a smooth flat plate. This velocity field was used as a surrogate of the 3-D turbulent conditions close to the bed in streams. We first checked that the basic turbulence statistics for this velocity field could be used to approximate those in an open-channel flow. We then analyzed the interaction of the sediment and the turbulence for a single and multiple particles. We compared numerical results with experimental data obtained by Niño and García (1998b). We show that model predictions are in good agreement with existing data, in the sand size range. © 2011 ASCE.

  16. Theoretical efficiency limit for a two-terminal multi-junction "step-cell" using detailed balance method

    Science.gov (United States)

    Abdul Hadi, Sabina; Fitzgerald, Eugene A.; Nayfeh, Ammar

    2016-02-01

    Here we present detailed balance efficiency limit for a novel two-terminal dual and triple junction "step-cell" under AM 1.5G and AM 0 incident spectrums. The step-cell is a multi-junction (MJ) solar cell in which part of the top cell is removed, exposing some of the bottom cell area to unfiltered incident light, thus increasing bottom cell's photogenerated current. Optical generation of the bottom cell is modeled in two parts: step part, limited by the bottom cell bandgap, and conventional part, additionally limited by the top cell absorption. Our results show that conventionally designed MJ cell with optimized bandgap combination of 1.64 eV/0.96 eV for dual junction and 1.91 eV/1.37 eV/0.93 eV for triple junction has the highest theoretical efficiency limit. However, the step-cell design provides significant efficiency improvement for cells with non-optimum bandgap values. For example, for 1.41 eV ( ˜GaAs)/Si dual junction under AM 1.5G, efficiency limit increases from ˜21% in a conventional design to 38.7% for optimized step-cell. Similar benefits are observed for three-junction step-cell and for AM 0 spectrum studied here. Step-cell relaxes bandgap requirements for efficient MJ solar cells, providing an opportunity for a wider selection of materials and cost reduction.

  17. Advances in the theory and application of BSF cells. [including electrical resistivity and photovoltaic cells

    Science.gov (United States)

    Mandelkorn, J.; Lamneck, J. H.

    1975-01-01

    The characteristics and behavior of p(+), p solar cells were investigated. The p(+), p cells were made by the removal of the n(+) surface layers from n(+), p p(+), BSF cells followed by application of a suitable contact to the resultant p(+), p structures. The open circuit voltage of p(+), p cells was found to increase with increasing 'p' bulk resistivity. The measured open circuit velocity-temperature coefficients were positive and increased with increasing resistivity. An outline of prior limitations in solar cell design is presented, and the removal of these limitations through use of BSF effects is pointed out. The study of BSF effects made feasible production of very thin high efficiency silicon cells as well as high resistivity-high efficiency cells, two desirable types of silicon cells which were previously impossible to make.

  18. Detailed Analysis of Temperature Characteristics of an InGaP/InGaAs/Ge Triple-Junction Solar Cell

    Science.gov (United States)

    Nishioka, Kensuke; Sueto, Tsuyoshi; Uchida, Masaki; Ota, Yasuyuki

    2010-06-01

    Temperature characteristics of an InGaP/InGaAs/Ge triple-junction solar cell were analyzed in detail using an equivalent circuit calculation. The current-voltage ( I- V) characteristics of single-junction solar cells (InGaP, InGaAs, Ge solar cells) were measured at various temperatures. Fitting of I- V curves between measured and calculated data was carried out, and the diode parameters and temperature exponents of the single-junction solar cells were extracted. The parameters for each single-junction solar cell were used in the equivalent circuit model for the triple-junction solar cell, and calculations of solar cell performance were carried out. Measured and calculated results of the I- V characteristics at various temperatures agreed well.

  19. Detailed analysis of the cell-inactivation mechanism by accelerated protons and light ions

    CERN Document Server

    Kundrát, P

    2006-01-01

    Published survival data for V79 cells irradiated by monoenergetic protons, helium-3, carbon, and oxygen ions and for CHO cells irradiated by carbon ions have been analyzed using the probabilistic two-stage model of cell inactivation. Three different classes of DNA damages formed by traversing particles have been distinguished, namely severe single-track damages which might lead to cell inactivation directly, less severe damages where cell inactivation is caused by their combinations, and damages of negligible severity that can be repaired easily. Probabilities of single ions to form these damages have been assessed in dependence on their linear energy transfer (LET) values. Damage induction probabilities increase with atomic number and LET. While combined damages play crucial role at lower LET values, single-track damages dominate in high-LET regions. The yields of single-track lethal damages for protons have been compared with the Monte Carlo estimates of complex DNA lesions, indicating that lethal events co...

  20. Detailed analysis of an endoreversible fuel cell : Maximum power and optimal operating temperature determination

    CERN Document Server

    Vaudrey, A; Lanzetta, F; Glises, R

    2009-01-01

    Producing useful electrical work in consuming chemical energy, the fuel cell have to reject heat to its surrounding. However, as it occurs for any other type of engine, this thermal energy cannot be exchanged in an isothermal way in finite time through finite areas. As it was already done for various types of systems, we study the fuel cell within the finite time thermodynamics framework and define an endoreversible fuel cell. Considering different types of heat transfer laws, we obtain an optimal value of the operating temperature, corresponding to a maximum produced power. This analysis is a first step of a thermodynamical approach of design of thermal management devices, taking into account performances of the whole system.

  1. Detailed analysis of bathocuproine layer for organic solar cells based on copper phthalocyanine and C60

    Science.gov (United States)

    Huang, Jiang; Yu, Junsheng; Lin, Hui; Jiang, Yadong

    2009-04-01

    The electrical characteristics of double heterojunction organic solar cells with various thicknesses of bathocuproine (BCP) as an exciton-blocking layer (EBL) were studied. A theoretical analysis using optical transfer matrix theory was carried out to obtain insight into how the EBL influences light-absorbing and exciton-transporting properties in the organic layers. The results showed that by employing an 8-10 nm BCP layer exciton density was increased by 46%. By using an improved equivalent circuit model, photovoltaic characteristics were parametrized and modeled, which revealed that the thin BCP layer solar cells was also discussed.

  2. A detailed analysis of cyclin A accumulation at the G(1)/S border in normal and transformed cells.

    Science.gov (United States)

    Erlandsson, F; Linnman, C; Ekholm, S; Bengtsson, E; Zetterberg, A

    2000-08-25

    The temporal relationship between cyclin A accumulation and the onset of DNA replication was analyzed in detail. Five untransformed and nine transformed asynchronously growing cell cultures were investigated using a triple immunofluorescence staining protocol combined with computerized evaluation of staining intensities in individual cells. The simultaneous staining of BrdU, cyclin A, and cyclin E made it possible to determine the cell cycle position of each cell investigated. Cells at the G(1)/S border were identified on the basis of cyclin E content and were further analyzed with respect to cyclin A and BrdU content. A method was developed to calculate objective thresholds defining the highest staining intensity found in the negative cells in the population. Using the thresholds we could distinguish cells with minute amounts of cyclin A and BrdU from truly negative cells. We show that the onset of cyclin A accumulation and the start of DNA replication occurs at the same time, or deviating by a few minutes at the most. We also show that cyclin A accumulates continuously during S. This study clearly demonstrates that nuclear cyclin A can be used as a reliable marker for the S and G(2) phases in both normal and transformed interphase cells. PMID:10942581

  3. Detailed interrogation of trypanosome cell biology via differential organelle staining and automated image analysis

    Directory of Open Access Journals (Sweden)

    Wheeler Richard J

    2012-01-01

    Full Text Available Abstract Background Many trypanosomatid protozoa are important human or animal pathogens. The well defined morphology and precisely choreographed division of trypanosomatid cells makes morphological analysis a powerful tool for analyzing the effect of mutations, chemical insults and changes between lifecycle stages. High-throughput image analysis of micrographs has the potential to accelerate collection of quantitative morphological data. Trypanosomatid cells have two large DNA-containing organelles, the kinetoplast (mitochondrial DNA and nucleus, which provide useful markers for morphometric analysis; however they need to be accurately identified and often lie in close proximity. This presents a technical challenge. Accurate identification and quantitation of the DNA content of these organelles is a central requirement of any automated analysis method. Results We have developed a technique based on double staining of the DNA with a minor groove binding (4'', 6-diamidino-2-phenylindole (DAPI and a base pair intercalating (propidium iodide (PI or SYBR green fluorescent stain and color deconvolution. This allows the identification of kinetoplast and nuclear DNA in the micrograph based on whether the organelle has DNA with a more A-T or G-C rich composition. Following unambiguous identification of the kinetoplasts and nuclei the resulting images are amenable to quantitative automated analysis of kinetoplast and nucleus number and DNA content. On this foundation we have developed a demonstrative analysis tool capable of measuring kinetoplast and nucleus DNA content, size and position and cell body shape, length and width automatically. Conclusions Our approach to DNA staining and automated quantitative analysis of trypanosomatid morphology accelerated analysis of trypanosomatid protozoa. We have validated this approach using Leishmania mexicana, Crithidia fasciculata and wild-type and mutant Trypanosoma brucei. Automated analysis of T. brucei

  4. Specification of region-specific neurons including forebrain glutamatergic neurons from human induced pluripotent stem cells.

    Directory of Open Access Journals (Sweden)

    Hui Zeng

    Full Text Available BACKGROUND: Directed differentiation of human induced pluripotent stem cells (hiPSC into functional, region-specific neural cells is a key step to realizing their therapeutic promise to treat various neural disorders, which awaits detailed elucidation. METHODOLOGY/PRINCIPAL FINDINGS: We analyzed neural differentiation from various hiPSC lines generated by others and ourselves. Although heterogeneity in efficiency of neuroepithelial (NE cell differentiation was observed among different hiPSC lines, the NE differentiation process resembles that from human embryonic stem cells (hESC in morphology, timing, transcriptional profile, and requirement for FGF signaling. NE cells differentiated from hiPSC, like those from hESC, can also form rostral phenotypes by default, and form the midbrain or spinal progenitors upon caudalization by morphogens. The rostrocaudal neural progenitors can further mature to develop forebrain glutamatergic projection neurons, midbrain dopaminergic neurons, and spinal motor neurons, respectively. Typical ion channels and action potentials were recorded in the hiPSC-derived neurons. CONCLUSIONS/SIGNIFICANCE: Our results demonstrate that hiPSC, regardless of how they were derived, can differentiate into a spectrum of rostrocaudal neurons with functionality, which supports the considerable value of hiPSC for study and treatment of patient-specific neural disorders.

  5. ISOLATION OF HEPATIC OVAL CELLS FROM DIFFERENT MODEL RATS INCLUDING DIABETIC RATS

    Institute of Scientific and Technical Information of China (English)

    LU Ying-li; YE Ting-ting; XIA Fang-zhen; WANG Ning-jian; YANG Hua; CHEN Yi

    2009-01-01

    Objective To acquire oval cells (progenitor stem cells) from adult rat liver of different models including diabetic rats. Methods Thirty Sprague-Dawley (SD) rats were divided into 5 groups randomly: control, 2-acetylaminofluorene (2-AAF), 2-AAF+partial hepatectomy (PH), 2-AAF+carbon tetrachloride (CCl4), and diabetic groups. As two-step collagenase perfusion protocol of Seglen, oval cells were isolated by Percoll density gradient centrifugation. Thy1.1 positive cells were sorted by flow cytometry, and then cultured in Dulbeccos minimum Eagles medium (DMEM). Immunofluorescence staining was applied to labelling Thy1.1. Results Different rates of Thy1.1 positive oval cells were found in different rat model groups: 0.5% in 2-AAF, 0.3% in 2-hAAF+PH, 0.2% in 2-AAF+CCl4 , 0.1% in diabetic, and 0.0% in control. Isolated cells adhered to plate with fusiform or polygon as epithelial cells. Conclusion Progenitor stem cells exist in injured liver tissue including those from diabetic rats.

  6. Detailed analysis of ultrathin fluorescent red dye interlayer for organic photovoltaic cells

    Institute of Scientific and Technical Information of China (English)

    Zang Yue; Yu Jun-Sheng; Wang Na-Na; Jiang Ya-Dong

    2011-01-01

    The influence of an ultrathin 4-(dicyanomethylene)-2-t-butyl-6-(1,1,7,7-tetramethyljulolidyl-9-enyl)-4H-pyran (DCJTB) fluorescent dye layer at donor/acceptor heterojunction on the performance of small-molecule organic photovoltaic (OPV) cell is studied. The structure of OPV cell is of indium-tin oxide (ITO)/copper phthalocyanine (CuPc)/DCJTB/fullerene (C60)/bathophenanthroline (Bphen)/Ag. The results show that open circuit voltage (Voc)increases to 0.57 V as the fihn thicknees of DCJTB layer increases from 0.2 to 2.0 nm. By using an equivalent circuit model, the enhancement of Voc is found to be attributed to the reduced reverse saturation current density (Js) which is due to the lower highest occupied molecular orbital (HOMO) level in DCJTB than that in CuPc. Also, the short circuit current density (Jsc) is affected when the DCJTB layer becomes thicker, resulting from the high series resistance RsA due to the low charge carrier mobility of fluorescent red dye.

  7. Dynamics Analysis of an HIV Infection Model including Infected Cells in an Eclipse Stage

    Directory of Open Access Journals (Sweden)

    Shengyu Zhou

    2013-01-01

    Full Text Available In this paper, an HIV infection model including an eclipse stage of infected cells is considered. Some quicker cells in this stage become productively infected cells, a portion of these cells are reverted to the uninfected class, and others will be latent down in the body. We consider CTL-response delay in this model and analyze the effect of time delay on stability of equilibrium. It is shown that the uninfected equilibrium and CTL-absent infection equilibrium are globally asymptotically stable for both ODE and DDE model. And we get the global stability of the CTL-present equilibrium for ODE model. For DDE model, we have proved that the CTL-present equilibrium is locally asymptotically stable in a range of delays and also have studied the existence of Hopf bifurcations at the CTL-present equilibrium. Numerical simulations are carried out to support our main results.

  8. A POROELASTIC MODEL FOR CELL CRAWLING INCLUDING MECHANICAL COUPLING BETWEEN CYTOSKELETAL CONTRACTION AND ACTIN POLYMERIZATION.

    Science.gov (United States)

    Taber, L A; Shi, Y; Yang, L; Bayly, P V

    2011-01-01

    Much is known about the biophysical mechanisms involved in cell crawling, but how these processes are coordinated to produce directed motion is not well understood. Here, we propose a new hypothesis whereby local cytoskeletal contraction generates fluid flow through the lamellipodium, with the pressure at the front of the cell facilitating actin polymerization which pushes the leading edge forward. The contraction, in turn, is regulated by stress in the cytoskeleton. To test this hypothesis, finite element models for a crawling cell are presented. These models are based on nonlinear poroelasticity theory, modified to include the effects of active contraction and growth, which are regulated by mechanical feedback laws. Results from the models agree reasonably well with published experimental data for cell speed, actin flow, and cytoskeletal deformation in migrating fish epidermal keratocytes. The models also suggest that oscillations can occur for certain ranges of parameter values. PMID:21765817

  9. Myc and Miz-1 have coordinate genomic functions including targeting Hox genes in human embryonic stem cells

    Directory of Open Access Journals (Sweden)

    Varlakhanova Natalia

    2011-11-01

    Full Text Available Abstract Background A proposed role for Myc in maintaining mouse embryonic stem (ES cell pluripotency is transcriptional repression of key differentiation-promoting genes, but detail of the mechanism has remained an important open topic. Results To test the hypothesis that the zinc finger protein Miz-1 plays a central role, in the present work we conducted chromatin immunoprecipitation/microarray (ChIP-chip analysis of Myc and Miz-1 in human ES cells, finding homeobox (Hox genes as the most significant functional class of Miz-1 direct targets. Miz-1 differentiation-associated target genes specifically lack acetylated lysine 9 and trimethylated lysine 4 of histone H3 (AcH3K9 and H3K4me3 9 histone marks, consistent with a repressed transcriptional state. Almost 30% of Miz-1 targets are also bound by Myc and these cobound genes are mostly factors that promote differentiation including Hox genes. Knockdown of Myc increased expression of differentiation genes directly bound by Myc and Miz-1, while a subset of the same genes is downregulated by Miz-1 loss-of-function. Myc and Miz-1 proteins interact with each other and associate with several corepressor factors in ES cells, suggesting a mechanism of repression of differentiation genes. Conclusions Taken together our data indicate that Miz-1 and Myc maintain human ES cell pluripotency by coordinately suppressing differentiation genes, particularly Hox genes. These data also support a new model of how Myc and Miz-1 function on chromatin.

  10. Model-based deconvolution of cell cycle time-series data reveals gene expression details at high resolution.

    Directory of Open Access Journals (Sweden)

    Dan Siegal-Gaskins

    2009-08-01

    Full Text Available In both prokaryotic and eukaryotic cells, gene expression is regulated across the cell cycle to ensure "just-in-time" assembly of select cellular structures and molecular machines. However, present in all time-series gene expression measurements is variability that arises from both systematic error in the cell synchrony process and variance in the timing of cell division at the level of the single cell. Thus, gene or protein expression data collected from a population of synchronized cells is an inaccurate measure of what occurs in the average single-cell across a cell cycle. Here, we present a general computational method to extract "single-cell"-like information from population-level time-series expression data. This method removes the effects of 1 variance in growth rate and 2 variance in the physiological and developmental state of the cell. Moreover, this method represents an advance in the deconvolution of molecular expression data in its flexibility, minimal assumptions, and the use of a cross-validation analysis to determine the appropriate level of regularization. Applying our deconvolution algorithm to cell cycle gene expression data from the dimorphic bacterium Caulobacter crescentus, we recovered critical features of cell cycle regulation in essential genes, including ctrA and ftsZ, that were obscured in population-based measurements. In doing so, we highlight the problem with using population data alone to decipher cellular regulatory mechanisms and demonstrate how our deconvolution algorithm can be applied to produce a more realistic picture of temporal regulation in a cell.

  11. Physical parameters, modeling, and methodological details in using IR laser pulses to warm frozen or vitrified cells ultra-rapidly.

    Science.gov (United States)

    Kleinhans, F W; Mazur, Peter

    2015-04-01

    We report additional details of the thermal modeling, selection of the laser, and construction of the Cryo Jig used for our ultra-rapid warming studies of mouse oocytes (Jin et al., 2014). A Nd:YAG laser operating at 1064 nm was selected to deliver short 1ms pulses of sufficient power to produce a warming rate of 1×10(7)°C/min from -190°C to 0°C. A special Cryo Jig was designed and built to rapidly remove the sample from LN2 and expose it to the laser pulse. India ink carbon black particles were required to increase the laser energy absorption of the sample. The thermal model reported here is more general than that previously reported. The modeling reveals that the maximum warming rate achievable via external warming across the cell membrane is proportional to (1/R(2)) where R is the cell radius.

  12. Induced Resistance to Ofatumumab Mediated Cell Clearance Mechanisms, Including Complement Dependent Cytotoxicity, in Chronic Lymphocytic Leukemia

    OpenAIRE

    Baig, Nisar A.; Taylor, Ronald P.; Lindorfer, Margaret A.; Church, Amy K.; LaPlant, Betsy R.; Pettinger, Adam M.; Shanafelt, Tait D.; Nowakowski, Grzegorz S.; Zent, Clive S.

    2014-01-01

    Ofatumumab (OFA), a human CD20 targeting mAb, kills B-lymphocytes utilizing the innate immune system including complement dependent cytotoxicity (CDC). The efficacy of OFA in patients with chronic lymphocytic leukemia (CLL) is limited by drug resistance, which is not well characterized. To better understand mechanisms of resistance, we prospectively studied CLL cells isolated from blood samples collected before and after in vivo exposure to the initial dose of OFA therapy in 25 patients under...

  13. A full model for simulation of electrochemical cells including complex behavior

    Energy Technology Data Exchange (ETDEWEB)

    Esperilla, J.J.; Felez, J.; Romero, G.; Carretero, A. [ETS Ingenieros Industriales, Universidad Politecnica de Madrid, Jose Gutierrez Abascal, 2, 28006 Madrid (Spain)

    2007-02-25

    This communication presents a model of electrochemical cells developed in order to simulate their electrical, chemical and thermal behavior showing the differences when thermal effects are or not considered in the charge-discharge process. The work presented here has been applied to the particular case of the Pb,PbSO{sub 4}H{sub 2}SO{sub 4} (aq)PbO{sub 2},Pb cell, which forms the basis of the lead-acid batteries so widely used in the automotive industry and as traction batteries in electric or hybrid vehicles. Each half-cell is considered independently in the model. For each half-cell, in addition to the main electrode reaction, a secondary reaction is considered: the hydrogen evolution reaction in the negative electrode and the oxygen evolution reaction in the positive. The equilibrium potential is calculated with the Nernst equation, in which the activity coefficients are fitted to an exponential function using experimental data. On the other hand, the two main mechanisms that produce the overpotential are considered, that is the activation or charge transfer and the diffusion mechanisms. First, an isothermal model has been studied in order to show the behavior of the main phenomena. A more complex model has also been studied including thermal behavior. This model is very useful in the case of traction batteries in electric and hybrid vehicles where high current intensities appear. Some simulation results are also presented in order to show the accuracy of the proposed models. (author)

  14. A full model for simulation of electrochemical cells including complex behavior

    Science.gov (United States)

    Esperilla, J. J.; Félez, J.; Romero, G.; Carretero, A.

    This communication presents a model of electrochemical cells developed in order to simulate their electrical, chemical and thermal behavior showing the differences when thermal effects are or not considered in the charge-discharge process. The work presented here has been applied to the particular case of the Pb,PbSO 4|H 2SO 4 (aq)|PbO 2,Pb cell, which forms the basis of the lead-acid batteries so widely used in the automotive industry and as traction batteries in electric or hybrid vehicles. Each half-cell is considered independently in the model. For each half-cell, in addition to the main electrode reaction, a secondary reaction is considered: the hydrogen evolution reaction in the negative electrode and the oxygen evolution reaction in the positive. The equilibrium potential is calculated with the Nernst equation, in which the activity coefficients are fitted to an exponential function using experimental data. On the other hand, the two main mechanisms that produce the overpotential are considered, that is the activation or charge transfer and the diffusion mechanisms. First, an isothermal model has been studied in order to show the behavior of the main phenomena. A more complex model has also been studied including thermal behavior. This model is very useful in the case of traction batteries in electric and hybrid vehicles where high current intensities appear. Some simulation results are also presented in order to show the accuracy of the proposed models.

  15. Detail and survey radioautographs

    Energy Technology Data Exchange (ETDEWEB)

    Wainwright, Wm.W.

    1949-04-19

    The much used survey or contact type of radioautograph is indispensible for a study of the gross distribution of radioactive materials. A detail radioautograph is equally indispensible. The radioautograph makes possible the determination of plutonium with respect to cells. Outlines of survey and detail techniques are given.

  16. Eukar yotic cell encystation and cancer cell dormancy:is a greater devil veiled in the details of a lesser evil?

    Institute of Scientific and Technical Information of China (English)

    Abdul Mannan Baig; Naveed Ahmed Khan; Farhat Abbas

    2015-01-01

    Cancer cell dormancy is the main cause of cancer recurrence and failure of therapy as dormant cells evade not only the anticancer drugs but also the host immune system. hTese dormant cells veil themselves from detection by imaging and/or using biomarkers, which imposes an additional problem in targeting such cells. A similar form of hibernation process known as encystation is studied in detail for pathogenic unicellular eukaryotic microorganisms. By examination using microarray gene expression proifles, immunocytochemistry tools, and siRNAs during the process of encystation, understanding the covert features of cancer cell dormancy as proposed could be possible. hTis knowledge can be extended to dormant cancer cells to uncover the mechanisms that underlie this ghost, yet dangerous state of human cancers. We propose a strategy to induce dormancy and exit this state by application of knowledge gained from the encystation induction and retrieval processes in pathogenic eukaryotic microorganisms. Given that early detection and characterization of dormant malignant tumor cells is important as a general strategy to monitor and prevent the development of overt metastatic disease, this homology may enable the design of therapies that could either awake the dormant cell from dormancy to make it available for therapies or prolong such a phase to make cancer appear as a chronic disease.

  17. Immune therapy including dendritic cell based therapy in chronic hepatitis B virus infection

    Institute of Scientific and Technical Information of China (English)

    Sk Md Fazle Akbar; Norio Horiike; Morikazu Onji

    2006-01-01

    Hepatitis B virus (HBV) infection is a global public health problem. Of the approximately 2 billion people who have been infected worldwide, more than 400 million are chronic carriers of HBV. Considerable numbers of chronic HBV carriers suffer from progressive liver diseases. In addition, all HBV carriers are permanent source of this virus. There is no curative therapy for chronic HBV carriers. Antiviral drugs are recommended for about 10% patients, however, these drugs are costly, have limited efficacy, and possess considerable side effects.Recent studies have shown that immune responses of the host to the HBV are critically involved at every stage of chronic HBV infection: (1) These influence acquisition of chronic HBV carrier state, (2) They are important in the context of liver damages, (3) Recovery from chronic HBV-related liver diseases is dependent on nature and extent of HBV-specific immune responses.However, induction of adequate levels of HBV-specific immune responses in chronic HBV carriers is difficult.During the last one decade, hepatitis B vaccine has been administered to chronic HBV carriers as a therapeutic approach (vaccine therapy). The present regimen of vaccine therapy is safe and cheap, but not so effective.A dendritic cell-based therapeutic vaccine has recently been developed for treating chronic HBV infection. In this review, we will discuss about the concept, scientific logics, strategies and techniques of development of HBV-specific immune therapies including vaccine therapy and dendritic cell-based vaccine therapy for treating chronic HBV infection.

  18. THULE: A detailed description

    International Nuclear Information System (INIS)

    This report describes the THULE scheme of lattice physics calculation which has been developed in FORTRAN for the IBM 7090. This scheme predicts the neutron flux over energy and space, for many groups and regions, together with reactivity and reaction rate edits for both a single lattice cell and a reactor core. This report describes in detail the input requirements for the THULE programme which forms the main part of the scheme. Brief descriptions of the 7090 programmes TED 6 and NOAH are included as appendices. TED 6 will produce the THULE edits from a WDSN output tape and NOAH is a version of the METHUSELAH programme which contains many of the THULE edits and will also produce input cards for THULE. (author)

  19. Particle-in-cell simulations for virtual cathode oscillator including foil ablation effects

    International Nuclear Information System (INIS)

    We have performed two- and three-dimensional, relativistic, electromagnetic, particle-in-cell simulations of an axially extracted virtual cathode oscillator (vircator). The simulations include, for the first time, self-consistent dynamics of the anode foil under the influence of the intense electron beam. This yields the variation of microwave output power as a function of time, including the role of anode ablation and anode-cathode gap closure. These simulations have been done using locally developed particle-in-cell (PIC) codes. The codes have been validated using two vircator designs available from the literature. The simulations reported in the present paper take account of foil ablation due to the intense electron flux, the resulting plasma expansion and shorting of the anode-cathode gap. The variation in anode transparency due to plasma formation is automatically taken into account. We find that damage is generally higher near the axis. Also, at all radial positions, there is little damage in the early stages, followed by a period of rapid erosion, followed in turn by low damage rates. A physical explanation has been given for these trends. As a result of gap closure due to plasma formation from the foil, the output microwave power initially increases, reaches a near-flat-top and then decreases steadily, reaching a minimum around 230 ns. This is consistent with a typical plasma expansion velocity of ∼2 cm/μs reported in the literature. We also find a significant variation in the dominant output frequency, from 6.3 to 7.6 GHz. This variation is small as long as the plasma density is small, up to ∼40 ns. As the AK gap starts filling with plasma, there is a steady increase in this frequency.

  20. Particle-in-cell simulations for virtual cathode oscillator including foil ablation effects

    Science.gov (United States)

    Singh, Gursharn; Chaturvedi, S.

    2011-06-01

    We have performed two- and three-dimensional, relativistic, electromagnetic, particle-in-cell simulations of an axially extracted virtual cathode oscillator (vircator). The simulations include, for the first time, self-consistent dynamics of the anode foil under the influence of the intense electron beam. This yields the variation of microwave output power as a function of time, including the role of anode ablation and anode-cathode gap closure. These simulations have been done using locally developed particle-in-cell (PIC) codes. The codes have been validated using two vircator designs available from the literature. The simulations reported in the present paper take account of foil ablation due to the intense electron flux, the resulting plasma expansion and shorting of the anode-cathode gap. The variation in anode transparency due to plasma formation is automatically taken into account. We find that damage is generally higher near the axis. Also, at all radial positions, there is little damage in the early stages, followed by a period of rapid erosion, followed in turn by low damage rates. A physical explanation has been given for these trends. As a result of gap closure due to plasma formation from the foil, the output microwave power initially increases, reaches a near-flat-top and then decreases steadily, reaching a minimum around 230 ns. This is consistent with a typical plasma expansion velocity of ˜2 cm/μs reported in the literature. We also find a significant variation in the dominant output frequency, from 6.3 to 7.6 GHz. This variation is small as long as the plasma density is small, up to ˜40 ns. As the AK gap starts filling with plasma, there is a steady increase in this frequency.

  1. LOCKE Detailed Specification Tables

    CERN Document Server

    Menezo, Lucia G; Gregorio, Jose-Angel

    2012-01-01

    This document shows the detailed specification of LOCKE coherence protocol for each cache controller, using a table-based technique. This representation provides clear, concise visual information yet includes sufficient detail (e.g., transient states) arguably lacking in the traditional, graphical form of state diagrams.

  2. A practical algorithm for optimal operation management of distribution network including fuel cell power plants

    Energy Technology Data Exchange (ETDEWEB)

    Niknam, Taher; Meymand, Hamed Zeinoddini; Nayeripour, Majid [Electrical and Electronic Engineering Department, Shiraz University of Technology, Shiraz (Iran)

    2010-08-15

    Fuel cell power plants (FCPPs) have been taken into a great deal of consideration in recent years. The continuing growth of the power demand together with environmental constraints is increasing interest to use FCPPs in power system. Since FCPPs are usually connected to distribution network, the effect of FCPPs on distribution network is more than other sections of power system. One of the most important issues in distribution networks is optimal operation management (OOM) which can be affected by FCPPs. This paper proposes a new approach for optimal operation management of distribution networks including FCCPs. In the article, we consider the total electrical energy losses, the total electrical energy cost and the total emission as the objective functions which should be minimized. Whereas the optimal operation in distribution networks has a nonlinear mixed integer optimization problem, the optimal solution could be obtained through an evolutionary method. We use a new evolutionary algorithm based on Fuzzy Adaptive Particle Swarm Optimization (FAPSO) to solve the optimal operation problem and compare this method with Genetic Algorithm (GA), Particle Swarm Optimization (PSO), Differential Evolution (DE), Ant Colony Optimization (ACO) and Tabu Search (TS) over two distribution test feeders. (author)

  3. Inactivation of Ricin Toxin by Nanosecond Pulsed Electric Fields Including Evidences from Cell and Animal Toxicity

    Science.gov (United States)

    Wei, Kai; Li, Wei; Gao, Shan; Ji, Bin; Zang, Yating; Su, Bo; Wang, Kaile; Yao, Maosheng; Zhang, Jue; Wang, Jinglin

    2016-01-01

    Ricin is one of the most toxic and easily produced plant protein toxin extracted from the castor oil plant, and it has been classified as a chemical warfare agent. Here, nanosecond pulsed electric fields (nsPEFs) at 30 kV/cm (pulse durations: 10 ns, 100 ns, and 300 ns) were applied to inactivating ricin up to 4.2 μg/mL. To investigate the efficacy, cells and mice were tested against the ricin treated by the nsPEFs via direct intraperitoneal injection and inhalation exposure. Results showed that nsPEFs treatments can effectively reduce the toxicity of the ricin. Without the nsPEFs treatment, 100% of mice were killed upon the 4 μg ricin injection on the first day, however 40% of the mice survived the ricin treated by the nsPEFs. Compared to injection, inhalation exposure even with higher ricin dose required longer time to observe mice fatality. Pathological observations revealed damages to heart, lung, kidney, and stomach after the ricin exposure, more pronounced for lung and kidney including severe bleeding. Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) and circular dichroism (CD) analyses revealed that although the primary structure of ricin was not altered, its secondary structures (beta-sheet and beta-turn) underwent transition upon the nsPEFs treatment.

  4. Inactivation of Ricin Toxin by Nanosecond Pulsed Electric Fields Including Evidences from Cell and Animal Toxicity.

    Science.gov (United States)

    Wei, Kai; Li, Wei; Gao, Shan; Ji, Bin; Zang, Yating; Su, Bo; Wang, Kaile; Yao, Maosheng; Zhang, Jue; Wang, Jinglin

    2016-01-05

    Ricin is one of the most toxic and easily produced plant protein toxin extracted from the castor oil plant, and it has been classified as a chemical warfare agent. Here, nanosecond pulsed electric fields (nsPEFs) at 30 kV/cm (pulse durations: 10 ns, 100 ns, and 300 ns) were applied to inactivating ricin up to 4.2 μg/mL. To investigate the efficacy, cells and mice were tested against the ricin treated by the nsPEFs via direct intraperitoneal injection and inhalation exposure. Results showed that nsPEFs treatments can effectively reduce the toxicity of the ricin. Without the nsPEFs treatment, 100% of mice were killed upon the 4 μg ricin injection on the first day, however 40% of the mice survived the ricin treated by the nsPEFs. Compared to injection, inhalation exposure even with higher ricin dose required longer time to observe mice fatality. Pathological observations revealed damages to heart, lung, kidney, and stomach after the ricin exposure, more pronounced for lung and kidney including severe bleeding. Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) and circular dichroism (CD) analyses revealed that although the primary structure of ricin was not altered, its secondary structures (beta-sheet and beta-turn) underwent transition upon the nsPEFs treatment.

  5. Detailed uranium geochemical stream sediment survey data release for selected portions of the Craig NTMS Quadrangle, Colorado, and Rawlins NTMS Quadrangle, Wyoming, including concentrations of forty-five additional elements

    International Nuclear Information System (INIS)

    This detailed study was undertaken at the request of the NURE Program Office, Grand Junction, Colorado, to aid in the evaluation of the uranium potential of the Browns Park and North Park formations in the area east of the Maybell-Lay, Colorado, uranium district and southeast of the Baggs, Wyoming, uranium district. A total of 1199 stream sediment samples was collected from 1199 locations in five areas in the quadrangle. The areas are designated Spring Creek, Hahns Peak, Elkhead Mountains, Yampa, and White River. All samples were collected at a nominal density of one sample location per 1.4 km2 Standardized field, analytical, and data base management procedures used during the sampling program are described briefly in the appendixes of this report. Elemental concentration, field measurement, weather, geologic, and geographic data for each sample location are listed in Appendix I. Uranium values range from 1.47 to 27.34 and have a mean of 3.53 ppM. Uranium/thorium ratios for sediment samples are also included in Appendix I. Sediments were analyzed for uranium and thorium as well as aluminum, antimony, arsenic, barium, beryllium, bismuth, cadmium, calcium, cerium, cesium, chlorine, chromium, cobalt, copper, dysprosium, europium, gold, hafnium, iron, lanthanum, lead, lithium, lutetium, magnesium, manganese, nickel, niobium, potassium, rubidium, samarium, scandium, selenium, silver, sodium, strontium, tantalum, terbium, tin, titanium, tungsten, vanadium, ytterbium, zinc, and zirconium (Appendix I). All sediments were analyzed for uranium by delayed neutron counting. Other elemental concentrations in sediments were determined by neutron activation analysis for 31 elements, by x-ray fluorescence for 12 elements, and by arc-source emission spectrography for 2 elements. Analytical results for sediments are reported as ppM

  6. Insect biofuel cells using trehalose included in insect hemolymph leading to an insect-mountable biofuel cell.

    Science.gov (United States)

    Shoji, Kan; Akiyama, Yoshitake; Suzuki, Masato; Hoshino, Takayuki; Nakamura, Nobuhumi; Ohno, Hiroyuki; Morishima, Keisuke

    2012-12-01

    In this paper, an insect biofuel cell (BFC) using trehalose included in insect hemolymph was developed. The insect BFC is based on trehalase and glucose oxidase (GOD) reaction systems which oxidize β-glucose obtained by hydrolyzing trehalose. First, we confirmed by LC-MS that a sufficient amount of trehalose was present in the cockroach hemolymph (CHL). The maximum power density obtained using the insect BFC was 6.07 μW/cm(2). The power output was kept more than 10 % for 2.5 h by protecting the electrodes with a dialysis membrane. Furthermore, the maximum power density was increased to 10.5 μW/cm(2) by using an air diffusion cathode. Finally, we succeeded in driving a melody integrated circuit (IC) and a piezo speaker by connecting five insect BFCs in series. The results indicate that the insect BFC is a promising insect-mountable battery to power environmental monitoring micro-tools.

  7. Methods of improving the efficiency of photovoltaic cells. [including X ray analysis

    Science.gov (United States)

    Loferski, J. J.; Roessler, B.; Crisman, E. E.; Chen, L. Y.; Kaul, R.

    1974-01-01

    Work on aluminum-alloyed silicon grating cells is continued. Optimization of the geometry (grating line width and spacing) confirms the analysis of such cells. A 1 sq cm grating cell was fabricated and its i-V characteristic was measured under an AMO solar simulator. It is found that the efficiency of this cell would be about 7.9%, if it were covered by the usual antireflection coating. The surface of the cell is not covered by a diffused junction. The response is blue shifted; the current is somewhat higher than that produced by a commercial Si cell. However, the open circuit voltage is low, and attempts to optimize the open circuit voltage of the aluminum-alloy junctions are described. A preliminary X-ray topographic examination of GaAs specimens of the type commonly used to make solar cells is studied. The X-ray study shows that the wafers are filled with regions having strain gradients, possibly caused by precipitates. It is possible that a correlation exists between the presence of low mechanical perfection and minority carrier diffusion lengths of GaAs crystals.

  8. Silicon quantum dot superlattice solar cell structure including silicon nanocrystals in a photogeneration layer

    OpenAIRE

    Yamada, Shigeru; Kurokawa, Yasuyoshi; Miyajima, Shinsuke; KONAGAI, MAKOTO

    2014-01-01

    The solar cell structure of n-type poly-silicon/5-nm-diameter silicon nanocrystals embedded in an amorphous silicon oxycarbide matrix (30 layers)/p-type hydrogenated amorphous silicon/Al electrode was fabricated on a quartz substrate. An open-circuit voltage and a fill factor of 518 mV and 0.51 in the solar cell were obtained, respectively. The absorption edge of the solar cell was 1.49 eV, which corresponds to the optical bandgap of the silicon nanocrystal materials, suggesting that it is po...

  9. Methods for the measurement of cell and tissue compatibility including tissue regeneration processes

    OpenAIRE

    C. Wiegand; Hipler, UC

    2008-01-01

    Biocompatibility is one of the main requirements for the safe use of medical devices. Determination of cytotoxicity is part of the initial evaluation stipulated by ISO standards for the biological evaluation of medical devices. The use of cell cultures to test the biocompatibility of drugs, biomaterials or treatment techniques used in various disciplines is gaining in importance. A wide variety of self-initiated and commercially available cell lines has been evaluated and used: cultured fibro...

  10. A Circuit Model for CMOS Hall Cells Performance Evaluation including Temperature Effects

    Directory of Open Access Journals (Sweden)

    Maria-Alexandra Paun

    2013-01-01

    Full Text Available In order to provide the information on their Hall voltage, sensitivity, and drift with temperature, a new simpler lumped circuit model for the evaluation of various Hall cells has been developed. In this sense, the finite element model proposed by the authors in this paper contains both geometrical parameters (dimensions of the cells and physical parameters such as the mobility, conductivity, Hall factor, carrier concentration, and the temperature influence on them. Therefore, a scalable finite element model in Cadence, for behavior simulation in circuit environment of CMOS Hall effect devices, with different shapes and technologies assessing their performance, has been elaborated.

  11. Fabrication of Polymer Solar Cells Using Aqueous Processing for All Layers Including the Metal Back Electrode

    DEFF Research Database (Denmark)

    Søndergaard, Roar; Helgesen, Martin; Jørgensen, Mikkel;

    2011-01-01

    The challenges of printing all layers in polymer solar cells from aqueous solution are met by design of inks for the electron-, hole-, active-, and metallic back electrode-layers. The conversion of each layer to an insoluble state after printing enables multilayer formation from the same solvent...

  12. Detailed kinetics of EBV-specific CD4(+) and CD8(+) T cells during primary EBV infection in a kidney transplant patient

    NARCIS (Netherlands)

    E.R.W.A.N. Piriou; K. van Dort; J.F.L. Weel; F.J. Bemelman; L.E. Gamadia; M.H.J. van Oers; D. van Baarle

    2006-01-01

    The etiology of infectious mononucleosis is poorly understood and usually detected many weeks after infection. Here, we present a unique case of primary symptomatic EBV infection after kidney transplantation, in whom we analyzed both EBV-specific CD4(+) and CD8(+)T cells in detail from the moment of

  13. Global Bifurcation Structure and Variability of Pacemaker Rhythm in a Detailed Model of Cardiac Sinoatrial Node Cells

    Science.gov (United States)

    Pan, Zhenxing; Doi, Shinji

    As a cardiac pacemaker, sinoatrial node spontaneously generates periodic electrical signals (action potentials) in its cells. The action potential generation is deeply related to various ion channels in cell membranes, and the abnormalities of ion channels cause sinus arrhythmia. We use the Zhang model of sinoatrial node cells to investigate the relation between pacemaker rhythm (frequency of action potential generation) and ion channels. The Zhang model is described by the Hodgkin-Huxley-type nonlinear ordinary differential equations, and its parameter values vary between periphery and center cells of sinoatrial node. We analyze the bifurcation structure of the Zhang model, and investigate the variability of pacemaker rhythm and its sensitivity on ion channel conductance changes for both periphery and center cells. Moreover, these results are compared with the previous results of another sinoatrial node cell model: Yanagihara-Noma-Irisawa model.

  14. Cancer risks related to low-level RF/MW exposures, including cell phones.

    Science.gov (United States)

    Szmigielski, Stanislaw

    2013-09-01

    For years, radiofrequency (RF) and microwave (MW) radiations have been applied in the modern world. The rapidly increasing use of cellular phones called recent attention to the possible health risks of RF/MW exposures. In 2011, a group of international experts organized by IARC (International Agency for Research on Cancer in Lyon) concluded that RF/MW radiations should be listed as a possible carcinogen (group 2B) for humans. Three meta-analyses of case-control studies have concluded that using cell phones for more than ten years was associated with an increase in the overall risk of developing a brain tumor. The Interphone Study, the largest health-related case-control international study of use of cell phones and head and neck tumors, showed no statistically significant increases in brain cancers related to higher amounts of cell phone use, but excess risk in a small subgroup of more heavily exposed users associated with latency and laterality was reported. So far, the published studies do not show that mobile phones could for sure increase the risk of cancer. This conclusion is based on the lack of a solid biological mechanism, and the fact that brain cancer rates are not going up significantly. However, all of the studies so far have weaknesses, which make it impossible to entirely rule out a risk. Mobile phones are still a new technology and there is little evidence about effects of long-term use. For this reason, bioelectromagnetic experts advise application of a precautionary resources. It suggests that if people want to use a cell phone, they can choose to minimize their exposure by keeping calls short and preferably using hand-held sets. It also advises discouraging children from making non essential calls as well as also keeping their calls short.

  15. 25 Years of Tension over Actin Binding to the Cadherin Cell Adhesion Complex: The Devil is in the Details.

    Science.gov (United States)

    Nelson, W James; Weis, William I

    2016-07-01

    Over the past 25 years, there has been a conceptual (re)evolution in understanding how the cadherin cell adhesion complex, which contains F-actin-binding proteins, binds to the actin cytoskeleton. There is now good synergy between structural, biochemical, and cell biological results that the cadherin-catenin complex binds to F-actin under force. PMID:27166091

  16. Accurate expressions for solar cell fill factors including series and shunt resistances

    Science.gov (United States)

    Green, Martin A.

    2016-02-01

    Together with open-circuit voltage and short-circuit current, fill factor is a key solar cell parameter. In their classic paper on limiting efficiency, Shockley and Queisser first investigated this factor's analytical properties showing, for ideal cells, it could be expressed implicitly in terms of the maximum power point voltage. Subsequently, fill factors usually have been calculated iteratively from such implicit expressions or from analytical approximations. In the absence of detrimental series and shunt resistances, analytical fill factor expressions have recently been published in terms of the Lambert W function available in most mathematical computing software. Using a recently identified perturbative relationship, exact expressions in terms of this function are derived in technically interesting cases when both series and shunt resistances are present but have limited impact, allowing a better understanding of their effect individually and in combination. Approximate expressions for arbitrary shunt and series resistances are then deduced, which are significantly more accurate than any previously published. A method based on the insights developed is also reported for deducing one-diode fits to experimental data.

  17. Early lymphocyte recovery as a predictor of outcome, including relapse, after hematopoieticstem cell transplantation

    Directory of Open Access Journals (Sweden)

    Juliane Morando

    2012-01-01

    Full Text Available BACKGROUND: Despite advances in the treatment of acute leukemia, many patients need to undergo hematopoietic stem cell transplantation. Recent studies show that early lymphocyte recovery may be a predictor of relapse and survival in these patients. OBJECTIVE: To analyze the influence of lymphocyte recovery on Days +30 and +100 post-transplant on the occurrence of relapse and survival. METHODS: A descriptive, retrospective study was performed of 137 under 21-year-old patients who were submitted to hematopoietic stem cell transplantation for acute leukemia between 1995 and 2008. A lymphocyte count 0.3 x 10(9/L were considered adequate. Lymphocyte recovery was also analyzed on Day +100 with < 0.75 x 10(9/Land < 0.75 x 10(9/L being considered inadequate and adequate lymphocyte recovery, respectively. RESULTS: There was no significant difference in the occurrence of relapse between patients with inadequate and adequate lymphocyte recovery on Day +30 post-transplant. However, the transplant-related mortality was significantly higher in patients with inadequate recovery on Day +30. Patients with inadequate lymphocyte recovery on Day +30 had worse overall survival and relapse-free survival than patients with adequate recovery. There was no significant difference in the occurrence of infections and acute or chronic graft-versus-host disease. Patients with inadequate lymphocyte recovery on Day +100 had worse overall survival and relapse-free survival and a higher cumulative incidence of relapse. CONCLUSION: The evaluation of lymphocyte recovery on Day +30 is not a good predictor of relapse after transplant however patients with inadequate lymphocyte recovery had worse overall survival and relapse-free survival. Inadequate lymphocyte recovery on Day +100 is correlated with higher cumulative relapse as well as lower overall survival and relapse-free survival.

  18. Simulation study of GaAsP/Si tandem cells including the impact of threading dislocations on the luminescent coupling between the cells

    Science.gov (United States)

    Onno, Arthur; Harder, Nils-Peter; Oberbeck, Lars; Liu, Huiyun

    2016-03-01

    A model, derived from the detailed balance model from Shockley and Queisser, has been adapted to monolithically grown GaAsP/Si tandem dual junction solar cells. In this architecture, due to the difference of lattice parameters between the silicon bottom cell - acting as the substrate - and the GaAsP top cell, threading dislocations (TDs) arise at the IIIV/ Si interface and propagate in the top cell. These TDs act as non-radiative recombination centers, degrading the performances of the tandem cell. Our model takes into account the impact of TDs by integrating the NTT model developed by Yamaguchi et. al.. Two surface geometries have been investigated: flat and ideally textured. Finally the model considers the luminescent coupling (LC) between the cells due to reemitted photons from the top cell cascading to the bottom cell. Without dislocations, LC allows a greater flexibility in the cell design by rebalancing the currents between the two cells when the top cell presents a higher short-circuit current. However we show that, as the TD density (TDD) increases, nonradiative recombinations take over radiative recombinations in the top cell and the LC is quenched. As a result, nonoptimized tandem cells with higher short-circuit current in the top cell experience a very fast degradation of efficiency for TDDs over 104cm-2. On the other hand optimized cells with matching currents only experience a small efficiency drop for TDDs up to 105cm-2. High TDD cells therefore need to be current-matched for optimal performances as the flexibility due to LC is lost.

  19. Main: Clone Detail [KOME

    Lifescience Database Archive (English)

    Full Text Available Clone Detail Mapping Pseudomolecule data detail Detail information Mapping to the TIGR japonica Pseudomolecu...les kome_mapping_pseudomolecule_data_detail.zip kome_mapping_pseudomolecule_data_detail ...

  20. Cathode material comparison of thermal runaway behavior of Li-ion cells at different state of charges including over charge

    Science.gov (United States)

    Mendoza-Hernandez, Omar Samuel; Ishikawa, Hiroaki; Nishikawa, Yuuki; Maruyama, Yuki; Umeda, Minoru

    2015-04-01

    The analysis of Li-ion secondary cells under outstanding conditions, as overcharge and high temperatures, is important to determine thermal abuse characteristics of electroactive materials and precise risk assessments on Li-ion cells. In this work, the thermal runaway behavior of LiCoO2 and LiMn2O4 cathode materials were compared at different state of charges (SOCs), including overcharge, by carrying out accelerating rate calorimetry (ARC) measurements using 18650 Li-ion cells. Onset temperatures of self-heating reactions and thermal runaway behavior were identified, and by using these onset points thermal mapping plots were made. We were able to identify non-self-heating, self-heating and thermal runaway regions as a function of state of charge and temperature. The cell using LiMn2O4 cathode material was found to be more thermally stable than the cell using LiCoO2. In parallel with the ARC measurements, the electrochemical behavior of the cells was monitored by measuring the OCV and internal resistance of the cells. The electrochemical behavior of the cells showed a slightly dependency on SOC.

  1. Combining a BCL2 Inhibitor with the Retinoid Derivative Fenretinide Targets Melanoma Cells Including Melanoma Initiating Cells

    OpenAIRE

    Mukherjee, Nabanita; Reuland, Steven N.; Lu, Yan; Luo, Yuchun; Lambert, Karoline; Fujita, Mayumi; Robinson, William A.; Robinson, Steven E.; David A Norris; Yiqun G Shellman

    2014-01-01

    Investigations from multiple laboratories support the existence of melanoma initiating cells (MICs) that potentially contribute to melanoma's drug resistance. ABT-737, a small molecule BCL-2/BCL-XL/BCL-W inhibitor, is promising in cancer treatments, but not very effective against melanoma, with the anti-apoptotic protein MCL-1 as the main contributor to resistance. The synthetic retinoid fenretinide (4-HPR) has shown promise for treating breast cancers. Here, we tested whether the combination...

  2. Combination chemotherapy including high dose methotrexate and radiotherapy, in the treatment of small cell carcinoma of the lung

    International Nuclear Information System (INIS)

    Twenty-nine (88%) of thirty-three patients who were treated with multiple drug chemotherapy, including high dose methotrexate, and radiotherapy for small cell carcinoma of the lung showed significant improvement in their clinical condition and quality of life. Treatment was well tolerated and toxicity acceptable. Cerebral metastases were not detected in any patient on presentation and only developed in three patients (9%). Little information exists regarding the use of high dose methotrexate in small cell carcinoma of the lung. There is no evidence, on the data available, that high dose methotrexate is any more effective than conventional doses. (author)

  3. Detailed search for protein kinase(s) involved in plasma membrane H+-ATPase activity regulation of yeast cells.

    Science.gov (United States)

    Pereira, Renata R; Castanheira, Diogo; Teixeira, Janaina A; Bouillet, Leoneide E M; Ribeiro, Erica M C; Trópia, Maria M J; Alvarez, Florencia; Correa, Lygia F M; Mota, Bruno E F; Conceição, Luis Eduardo F R; Castro, Ieso M; Brandão, Rogelio L

    2015-03-01

    This study displays a screening using yeast strains deficient in protein kinases known to exist in Saccharomyces cerevisiae. From 95 viable single mutants, 20 mutants appear to be affected in the glucose-induced extracellular acidification. The mutants that are unaffected in calcium signaling were tested for their sensitivity to hygromycin B. Furthermore, we verified whether the remaining mutants produced enzymes that are appropriately incorporated at plasma membrane. Finally, we measure the kinetic properties of the enzyme in purified plasma membranes from glucose-starved as well as glucose-fermenting cells. We confirmed the kinase Ptk2 involvement in H(+)-ATPase regulation (increase of affinity for ATP). However, the identification of the kinase(s) responsible for phosphorylation that leads to an increase in Vmax appears to be more complex. Complementary experiments were performed to check how those protein kinases could be related to the control of the plasma membrane H(+)-ATPase and/or the potential membrane. In summary, our results did not permit us to identify the protein kinase(s) involved in regulating the catalytic efficiency of the plasma membrane H(+)-ATPase. Therefore, our results indicate that the current regulatory model based on the phosphorylation of two different sites located in the C-terminus tail of the enzyme could be inappropriate.

  4. Neuritogenic Lewis rat T cells use Tcrb chains that include a new Tcrb-V8 family member.

    Science.gov (United States)

    Zhang, X M; Esch, T R; Clark, L; Gregorian, S; Rostami, A; Otvos, L; Heber-Katz, E

    1994-01-01

    The P2 protein obtained from Schwann cells induces a population of T cells which, upon adoptive transfer, causes the disease experimental allergic neuritis (EAN), an animal model for Guillain-Barre syndrome. In this report, a truncated peptide, FR22, derived from a previously reported neuritogenic T-cell determinant, was used to generate from Lewis rats T cells that were shown to cause EAN. Since our previous studies showed that Tcrb-V8 was used by a majority of T-cell hybridomas specific for the neuritogenic peptide P26, which contains the FR22 sequence, we sequenced the Tcrb-V8+ mRNA from FR22-specific T-cell lines, and compared the sequences obtained with those obtained from similarly generated myelin basic protein (MBP) 68-88-specific Lewis rat T-cell lines. We found that in the EAN lines, several members of the Tcrb-V8 family were used, including a new family member, Tcrb-V8E. This was more diverse than the MBP-68-88-specific response in which only a single Tcrb-V8 family member was used. Also, in the EAN lines, the beta chain sequences did not show the same conserved junctional regions seen in the MBP lines. Thus, T-cell receptor beta chain usage in the response to this dominant neuritogenic peptide appears to be less restricted than the response to the dominant encephalitogenic determinant of MBP both in V region usage and in CDR3 usage. PMID:7521858

  5. Detailed analysis of the cell infiltrate and the expression of mediators of synovial inflammation and joint destruction in the synovium of patients with psoriatic arthritis: implications for treatment

    NARCIS (Netherlands)

    A.W.R. van Kuijk; P. Reinders-Blankert; T.J.M. Smeets; B.A.C. Dijkmans; P.P. Tak

    2006-01-01

    Background: The synovial tissue is a primary target of many inflammatory arthropathies, including psoriatic arthritis ( PsA). Identification of proinflammatory molecules in the synovium may help to identify potentially therapeutic targets. Objective: To investigate extensively the features of cell i

  6. Mesenchymal stem cell therapy ameliorates diabetic nephropathy via the paracrine effect of renal trophic factors including exosomes

    Science.gov (United States)

    Nagaishi, Kanna; Mizue, Yuka; Chikenji, Takako; Otani, Miho; Nakano, Masako; Konari, Naoto; Fujimiya, Mineko

    2016-01-01

    Bone marrow-derived mesenchymal stem cells (MSCs) have contributed to the improvement of diabetic nephropathy (DN); however, the actual mediator of this effect and its role has not been characterized thoroughly. We investigated the effects of MSC therapy on DN, focusing on the paracrine effect of renal trophic factors, including exosomes secreted by MSCs. MSCs and MSC-conditioned medium (MSC-CM) as renal trophic factors were administered in parallel to high-fat diet (HFD)-induced type 2 diabetic mice and streptozotocin (STZ)-induced insulin-deficient diabetic mice. Both therapies showed approximately equivalent curative effects, as each inhibited the exacerbation of albuminuria. They also suppressed the excessive infiltration of BMDCs into the kidney by regulating the expression of the adhesion molecule ICAM-1. Proinflammatory cytokine expression (e.g., TNF-α) and fibrosis in tubular interstitium were inhibited. TGF-β1 expression was down-regulated and tight junction protein expression (e.g., ZO-1) was maintained, which sequentially suppressed the epithelial-to-mesenchymal transition of tubular epithelial cells (TECs). Exosomes purified from MSC-CM exerted an anti-apoptotic effect and protected tight junction structure in TECs. The increase of glomerular mesangium substrate was inhibited in HFD-diabetic mice. MSC therapy is a promising tool to prevent DN via the paracrine effect of renal trophic factors including exosomes due to its multifactorial action. PMID:27721418

  7. Detailed Analysis of Apoptosis and Delayed Luminescence of Human Leukemia Jurkat T Cells after Proton Irradiation and Treatments with Oxidant Agents and Flavonoids

    Directory of Open Access Journals (Sweden)

    Irina Baran

    2012-01-01

    Full Text Available Following previous work, we investigated in more detail the relationship between apoptosis and delayed luminescence (DL in human leukemia Jurkat T cells under a wide variety of treatments. We used menadione and hydrogen peroxide to induce oxidative stress and two flavonoids, quercetin, and epigallocatechin gallate, applied alone or in combination with menadione or H2O2. 62 MeV proton beams were used to irradiate cells under a uniform dose of 2 or 10 Gy, respectively. We assessed apoptosis, cell cycle distributions, and DL. Menadione, H2O2 and quercetin were potent inducers of apoptosis and DL inhibitors. Quercetin decreased clonogenic survival and the NAD(PH level in a dose-dependent manner. Proton irradiation with 2 Gy but not 10 Gy increased the apoptotic rate. However, both doses induced a substantial G2/M arrest. Quercetin reduced apoptosis and prolonged the G2/M arrest induced by radiation. DL spectroscopy indicated that proton irradiation disrupted the electron flow within Complex I of the mitochondrial respiratory chain, thus explaining the massive necrosis induced by 10 Gy of protons and also suggested an equivalent action of menadione and quercetin at the level of the Fe/S center N2, which may be mediated by their binding to a common site within Complex I, probably the rotenone-binding site.

  8. Quality of life, reproduction and sexuality after stem cell transplantation with partially T-cell-depleted grafts and after conditioning with a regimen including total body irradiation.

    NARCIS (Netherlands)

    Claessens, J.J.M.; Beerendonk, C.C.M.; Schattenberg, A.V.M.B.

    2006-01-01

    Thirty-four men and 36 women (median age 43 and 45 years, respectively) underwent stem cell transplantation (SCT) for acute leukaemia in first complete remission or chronic myelogenous leukaemia in first chronic phase between 1981 and 2001 from HLA-identical siblings. The conditioning regimen includ

  9. Numerical Simulation of Effects of Cloud Top Temperatures and Generating Cells on Secondary Ice Production in Stratiform Clouds with a Detailed Microphysical Model

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    This paper outlines a one-dimensional,heightdependent bin model with detailed microphysical processes in which ice splinters are produced by a riming process.The model is then applied to simulate the shift of particle size distribution effected by the secondary ice production process within clouds with different generating cells and cloud top temperatures.The result of model simulations reveals the general effects of cloud updrafts on increasing ice particle concentration by extending the residence time of ice particles in clouds and providing sufficiently large supercooled water droplets.The rimesplintering mechanism is more effective in clouds with lower ice seeding rates than those with higher rates.Evolutions of hydrometeor size distribution triggered by the rime-splintering mechanism indicate that the interaction between large ice particles and supercooled water drops adds a "second maximum" to the primary ice spectra.

  10. Fabrication of Mediatorless/Membraneless Glucose/Oxygen Based Biofuel Cell using Biocatalysts Including Glucose Oxidase and Laccase Enzymes

    Science.gov (United States)

    Christwardana, Marcelinus; Kim, Ki Jae; Kwon, Yongchai

    2016-01-01

    Mediatorless and membraneless enzymatic biofuel cells (EBCs) employing new catalytic structure are fabricated. Regarding anodic catalyst, structure consisting of glucose oxidase (GOx), poly(ethylenimine) (PEI) and carbon nanotube (CNT) is considered, while three cathodic catalysts consist of glutaraldehyde (GA), laccase (Lac), PEI and CNT that are stacked together in different ways. Catalytic activities of the catalysts for glucose oxidation and oxygen reduction reactions (GOR and ORR) are evaluated. As a result, it is confirmed that the catalysts work well for promotion of GOR and ORR. In EBC tests, performances of EBCs including 150 μm-thick membrane are measured as references, while those of membraneless EBCs are measured depending on parameters like glucose flow rate, glucose concentration, distance between two electrodes and electrolyte pH. With the measurements, how the parameters affect EBC performance and their optimal conditions are determined. Based on that, best maximum power density (MPD) of membraneless EBC is 102 ± 5.1 μW · cm−2 with values of 0.5 cc · min−1 (glucose flow rate), 40 mM (glucose concentration), 1 mm (distance between electrodes) and pH 3. When membrane and membraneless EBCs are compared, MPD of the membraneless EBC that is run at the similar operating condition to EBC including membrane is speculated as about 134 μW · cm−2. PMID:27426264

  11. Fabrication of Mediatorless/Membraneless Glucose/Oxygen Based Biofuel Cell using Biocatalysts Including Glucose Oxidase and Laccase Enzymes

    Science.gov (United States)

    Christwardana, Marcelinus; Kim, Ki Jae; Kwon, Yongchai

    2016-07-01

    Mediatorless and membraneless enzymatic biofuel cells (EBCs) employing new catalytic structure are fabricated. Regarding anodic catalyst, structure consisting of glucose oxidase (GOx), poly(ethylenimine) (PEI) and carbon nanotube (CNT) is considered, while three cathodic catalysts consist of glutaraldehyde (GA), laccase (Lac), PEI and CNT that are stacked together in different ways. Catalytic activities of the catalysts for glucose oxidation and oxygen reduction reactions (GOR and ORR) are evaluated. As a result, it is confirmed that the catalysts work well for promotion of GOR and ORR. In EBC tests, performances of EBCs including 150 μm-thick membrane are measured as references, while those of membraneless EBCs are measured depending on parameters like glucose flow rate, glucose concentration, distance between two electrodes and electrolyte pH. With the measurements, how the parameters affect EBC performance and their optimal conditions are determined. Based on that, best maximum power density (MPD) of membraneless EBC is 102 ± 5.1 μW · cm‑2 with values of 0.5 cc · min‑1 (glucose flow rate), 40 mM (glucose concentration), 1 mm (distance between electrodes) and pH 3. When membrane and membraneless EBCs are compared, MPD of the membraneless EBC that is run at the similar operating condition to EBC including membrane is speculated as about 134 μW · cm‑2.

  12. Fabrication of Mediatorless/Membraneless Glucose/Oxygen Based Biofuel Cell using Biocatalysts Including Glucose Oxidase and Laccase Enzymes.

    Science.gov (United States)

    Christwardana, Marcelinus; Kim, Ki Jae; Kwon, Yongchai

    2016-01-01

    Mediatorless and membraneless enzymatic biofuel cells (EBCs) employing new catalytic structure are fabricated. Regarding anodic catalyst, structure consisting of glucose oxidase (GOx), poly(ethylenimine) (PEI) and carbon nanotube (CNT) is considered, while three cathodic catalysts consist of glutaraldehyde (GA), laccase (Lac), PEI and CNT that are stacked together in different ways. Catalytic activities of the catalysts for glucose oxidation and oxygen reduction reactions (GOR and ORR) are evaluated. As a result, it is confirmed that the catalysts work well for promotion of GOR and ORR. In EBC tests, performances of EBCs including 150 μm-thick membrane are measured as references, while those of membraneless EBCs are measured depending on parameters like glucose flow rate, glucose concentration, distance between two electrodes and electrolyte pH. With the measurements, how the parameters affect EBC performance and their optimal conditions are determined. Based on that, best maximum power density (MPD) of membraneless EBC is 102 ± 5.1 μW · cm(-2) with values of 0.5 cc · min(-1) (glucose flow rate), 40 mM (glucose concentration), 1 mm (distance between electrodes) and pH 3. When membrane and membraneless EBCs are compared, MPD of the membraneless EBC that is run at the similar operating condition to EBC including membrane is speculated as about 134 μW · cm(-2). PMID:27426264

  13. Optimizing Staining Protocols for Laser Microdissection of Specific Cell Types from the Testis Including Carcinoma In Situ

    DEFF Research Database (Denmark)

    Sonne, Si Brask; Dalgaard, Marlene D; Nielsen, John Erik;

    2009-01-01

    protocols, and present two staining protocols for frozen sections, one for fast and specific staining of fetal germ cells, testicular carcinoma in situ cells, and other cells with embryonic stem cell-like properties that express the alkaline phosphatase, and one for specific staining of lipid droplet......Microarray and RT-PCR based methods are important tools for analysis of gene expression; however, in tissues containing many different cells types, such as the testis, characterization of gene expression in specific cell types can be severely hampered by noise from other cells. The laser...... microdissection technology allows for enrichment of specific cell types. However, when the cells are not morphologically distinguishable, it is necessary to use a specific staining method for the target cells. In this study we have tested different fixatives, storage conditions for frozen sections and staining...

  14. The Structure of Plant Cell Walls: I. The Macromolecular Components of the Walls of Suspension-cultured Sycamore Cells with a Detailed Analysis of the Pectic Polysaccharides.

    Science.gov (United States)

    Talmadge, K W; Keegstra, K; Bauer, W D; Albersheim, P

    1973-01-01

    This is the first in a series of papers dealing with the structure of cell walls isolated from suspension-cultured sycamore cells (Acer pseudoplatanus). These studies have been made possible by the availability of purified hydrolytic enzymes and by recent improvements in the techniques of methylation analysis. These techniques have permitted us to identify and quantitate the macromolecular components of sycamore cell walls. These walls are composed of 10% arabinan, 2% 3,6-linked arabinogalactan, 23% cellulose, 9% oligo-arabinosides (attached to hydroxyproline), 8% 4-linked galactan, 10% hydroxyproline-rich protein, 16% rhamnogalacturonan, and 21% xyloglucan.The structures of the pectic polymers (the neutral arabinan, the neutral galactan, and the acidic rhamnogalacturonan) were obtained, in part, by methylation analysis of fragments of these polymers which were released from the sycamore walls by the action of a highly purified endopolygalacturonase. The data suggest a branched arabinan and a linear 4-linked galactan occurring as side chains on the rhamnogalacturonan. Small amounts or pieces of a xyloglucan, the wall hemicellulose, appear to be covalently linked to some of the galactan chains. Thus, the galactan appears to serve as a bridge between the xyloglucan and rhamnogalacturonan components of the wall.The rhamnogalacturonan consists of an alpha-(1 --> 4)-linked galacturonan chain which is interspersed with 2-linked rhamnosyl residues. The rhamnosyl residues are not randomly distributed in the chain but probably occur in units of rhamnosyl- (1 --> 4)-galacturonosyl- (1 --> 2)-rhamnosyl. This sequence appears to alternate with a homogalacturonan sequence containing approximately 8 residues of 4-linked galacturonic acid. About half of the rhamnosyl residues are branched, having a substituent attached to carbon 4. This is likely to be the site of attachment of the 4-linked galactan.The hydroxyprolyl oligo-arabinosides of the hydroxyproline-rich glycoprotein

  15. Immunofluorescence Microscopy and mRNA Analysis of Human Embryonic Stem Cells (hESCs) Including Primary Cilia Associated Signaling Pathways

    DEFF Research Database (Denmark)

    Vestergaard, Maj Linea; Awan, Aashir; Warzecha, Caroline Becker;

    2016-01-01

    This chapter describes the procedures for immunofluorescence microscopy (IFM) and quantitative PCR (qPCR) analyses of human embryonic stem cells (hESCs) grown specifically under feeder-free conditions. A detailed protocol is provided outlining the steps from initially growing the cells, passaging...... onto 16-well glass chambers, and continuing with the general IFM and qPCR anlysis. The techniques are illustrated with results on cellular localization of transcriptional factors and components of the Hedgehog, Wnt, PDGF, and TGFβ signaling pathways to primary cilia in stem cell maintenance...

  16. Hybrid (particle in cell-fluid) simulation of ion-acoustic soliton generation including super-thermal and trapped electrons

    Energy Technology Data Exchange (ETDEWEB)

    Nopoush, M.; Abbasi, H. [Faculty of Physics, Amirkabir University of Technology, P. O. Box 15875-4413, Tehran (Iran, Islamic Republic of)

    2011-08-15

    The present paper is devoted to the simulation of the nonlinear disintegration of a localized perturbation into an ion-acoustic soliton in a plasma. Recently, this problem was studied by a simple model [H. Abbasi et al., Plasma Phys. Controlled Fusion 50, 095007 (2008)]. The main assumptions were (i) in the electron velocity distribution function (DF), the ion-acoustic soliton velocity was neglected in comparison to the electron thermal velocity, (ii) on the ion-acoustic evolution time-scale, the electron velocity DF was assumed to be stationary, and (iii) the calculation was restricted to the small amplitude case. In order to generalize the model, one has to consider the evolution of the electron velocity DF for finite amplitudes. For this purpose, a one dimensional electrostatic hybrid code, particle in cell (PIC)-fluid, was designed. It simulates the electrons dynamics by the PIC method and the cold ions dynamics by the fluid equations. The plasma contains a population of super-thermal electrons and, therefore, a Lorentzian (kappa) velocity DF is used to model the high energy tail in the electron velocity DF. Electron trapping is included in the simulation in view of their nonlinear resonant interaction with the localized perturbation. A Gaussian initial perturbation is used to model the localized perturbation. The influence of both the trapped and the super-thermal electrons on this process is studied and compared with the previous model.

  17. France; Financial Sector Assessment Program—Detailed Assessments of Observance of Standards and Codes including Banking Supervision, Insurance Regulation, Securities Legislation, Monetary and Financial Policy Transparency, Payments Systems, Securities Settlement, and Anti-Money Laundering and Combating the Financing of Terrorism

    OpenAIRE

    International Monetary Fund

    2005-01-01

    The Detailed Assessments of the Observance of Standards and Codes on France reviews compliance with Basel Core Principles for effective banking supervision. The French insurance market is notable for its broad range of distribution channels, which include tied agents, insurance brokers, salaried sales forces, direct writing mutuals, and financial institutions. France fulfills all prerequisites for effective payment clearing and settlement systems. Capital markets are large and sophisticated, ...

  18. Sericin, a protein derived from silkworms, accelerates the proliferation of several mammalian cell lines including a hybridoma

    OpenAIRE

    Terada, Satoshi; Nishimura, Taeko; Sasaki, Masahiro; Yamada, Hideyuki; Miki, Masao

    2002-01-01

    Sericin, a constituent of the silkworm cocoon, was added to the culture of four mammalian cell lines: murine hybridoma 2E3-O,human hepatoblastoma HepG2, human epithelial HeLa and human embryonal kidney 293 cells. The proliferation of all cell lineswas accelerated in the presence of sericin. The hybridoma cellline was further studied. The 2E3-O cell line was so well adapted to serum-free medium that both the proliferation rate and maximum cell density in serum-free ASF103 medium were higher th...

  19. A gene panel, including LRP12, is frequently hypermethylated in major types of B-cell lymphoma.

    Directory of Open Access Journals (Sweden)

    Nicole Bethge

    Full Text Available Epigenetic modifications and DNA methylation in particular, have been recognized as important mechanisms to alter gene expression in malignant cells. Here, we identified candidate genes which were upregulated after an epigenetic treatment of B-cell lymphoma cell lines (Burkitt's lymphoma, BL; Follicular lymphoma, FL; Diffuse large B-cell lymphoma, DLBCL activated B-cell like, ABC; and germinal center like, GCB and simultaneously expressed at low levels in samples from lymphoma patients. Qualitative methylation analysis of 24 candidate genes in cell lines revealed five methylated genes (BMP7, BMPER, CDH1, DUSP4 and LRP12, which were further subjected to quantitative methylation analysis in clinical samples from 59 lymphoma patients (BL, FL, DLBCL ABC and GCB; and primary mediastinal B-cell lymphoma, PMBL. The genes LRP12 and CDH1 showed the highest methylation frequencies (94% and 92%, respectively. BMPER (58%, DUSP4 (32% and BMP7 (22%, were also frequently methylated in patient samples. Importantly, all gene promoters were unmethylated in various control samples (CD19+ peripheral blood B cells, peripheral blood mononuclear cells and tonsils as well as in follicular hyperplasia samples, underscoring a high specificity. The combination of LRP12 and CDH1 methylation could successfully discriminate between the vast majority of the lymphoma and control samples, emphasized by receiver operating characteristic analysis with a c-statistic of 0.999. These two genes represent promising epigenetic markers which may be suitable for monitoring of B-cell lymphoma.

  20. Prognostic factors (including HPV status) for irradiation of locally advanced squamous cell carcinoma of the head and neck (SCCHN)

    Energy Technology Data Exchange (ETDEWEB)

    Rades, Dirk; Seibold, Nina D. [Lubeck Univ. (Germany). Dept. of Radiation Oncology; Gebhard, Maximilian P.; Noack, Frank; Thorns, Christoph [Lubeck Univ. (Germany). Inst. of Pathology; Schild, Steven E. [Mayo Clinic Scottsdale, AZ (United States). Dept. of Radiation Oncology

    2011-10-15

    The prognosis of patients with locally advanced squamous cell carcinoma of the head and neck (SCCHN) is generally poor. However, prognostic factors can help optimize the care for the individual patient. This study investigated potential prognostic factors, including HPV status, for locoregional control (LRC), metastases-free survival (MFS), and survival (OS). Twelve potential prognostic factors were investigated in 170 patients irradiated for stage III or IV SCCHN, including age ({<=} 60 vs > 60 years), gender, ECOG performance score (0-1 vs 2), preradiotherapy hemoglobin level (< 12 vs {>=} 12 g/dl), tumor site (oropharynx, oral cavity, hypopharynx, or larynx), histological grade (G1-2 vs G3), T category (T1-T2 vs T3-T4), N category (N0-N1 vs N2-N3), AJCC stage (III vs IV), surgery (no vs yes), and chemotherapy (no vs yes). On multivariate analysis, positive HPV status (RR 2.34; p = 0.014), ECOG performance score 0-1 (RR 1.94; p = 0.017), preRT hemoglobin {>=} 12 g/dl (RR 1.88; p = 0.018), T category T1-T2 (RR 2.72; p < 0.001), and surgery (RR 2.29; p = 0.007) were significantly associated with improved LRC. PreRT hemoglobin {>=} 12 g/dl (RR 1.98; p = 0.040) and T category T1-T2 (RR 3.33; p < 0.001) were significantly associated with improved MFS. Positive HPV status (RR 2.19; p = 0.019), pre-RT hemoglobin {>=} 12 g/dl (RR 2.15; p = 0.002), T category T1-T2 (RR 2.31; p = 0.002), and AJCC stage III (RR 1.91; p = 0.034) were significantly associated with improved OS. Improved treatment outcomes were significantly associated with positive HPV status, better performance status, lower tumor stage, and pretreatment hemoglobin levels {>=} 12 g/dl. These factors should be considered in future trials.

  1. Prognostic factors (including HPV status) for irradiation of locally advanced squamous cell carcinoma of the head and neck (SCCHN)

    International Nuclear Information System (INIS)

    The prognosis of patients with locally advanced squamous cell carcinoma of the head and neck (SCCHN) is generally poor. However, prognostic factors can help optimize the care for the individual patient. This study investigated potential prognostic factors, including HPV status, for locoregional control (LRC), metastases-free survival (MFS), and survival (OS). Twelve potential prognostic factors were investigated in 170 patients irradiated for stage III or IV SCCHN, including age (≤ 60 vs > 60 years), gender, ECOG performance score (0-1 vs 2), preradiotherapy hemoglobin level (< 12 vs ≥ 12 g/dl), tumor site (oropharynx, oral cavity, hypopharynx, or larynx), histological grade (G1-2 vs G3), T category (T1-T2 vs T3-T4), N category (N0-N1 vs N2-N3), AJCC stage (III vs IV), surgery (no vs yes), and chemotherapy (no vs yes). On multivariate analysis, positive HPV status (RR 2.34; p = 0.014), ECOG performance score 0-1 (RR 1.94; p = 0.017), preRT hemoglobin ≥ 12 g/dl (RR 1.88; p = 0.018), T category T1-T2 (RR 2.72; p < 0.001), and surgery (RR 2.29; p = 0.007) were significantly associated with improved LRC. PreRT hemoglobin ≥ 12 g/dl (RR 1.98; p = 0.040) and T category T1-T2 (RR 3.33; p < 0.001) were significantly associated with improved MFS. Positive HPV status (RR 2.19; p = 0.019), pre-RT hemoglobin ≥ 12 g/dl (RR 2.15; p = 0.002), T category T1-T2 (RR 2.31; p = 0.002), and AJCC stage III (RR 1.91; p = 0.034) were significantly associated with improved OS. Improved treatment outcomes were significantly associated with positive HPV status, better performance status, lower tumor stage, and pretreatment hemoglobin levels ≥ 12 g/dl. These factors should be considered in future trials.

  2. Crowdsourcing detailed flood data

    Science.gov (United States)

    Walliman, Nicholas; Ogden, Ray; Amouzad*, Shahrzhad

    2015-04-01

    Over the last decade the average annual loss across the European Union due to flooding has been 4.5bn Euros, but increasingly intense rainfall, as well as population growth, urbanisation and the rising costs of asset replacements, may see this rise to 23bn Euros a year by 2050. Equally disturbing are the profound social costs to individuals, families and communities which in addition to loss of lives include: loss of livelihoods, decreased purchasing and production power, relocation and migration, adverse psychosocial effects, and hindrance of economic growth and development. Flood prediction, management and defence strategies rely on the availability of accurate information and flood modelling. Whilst automated data gathering (by measurement and satellite) of the extent of flooding is already advanced it is least reliable in urban and physically complex geographies where often the need for precise estimation is most acute. Crowdsourced data of actual flood events is a potentially critical component of this allowing improved accuracy in situations and identifying the effects of local landscape and topography where the height of a simple kerb, or discontinuity in a boundary wall can have profound importance. Mobile 'App' based data acquisition using crowdsourcing in critical areas can combine camera records with GPS positional data and time, as well as descriptive data relating to the event. This will automatically produce a dataset, managed in ArcView GIS, with the potential for follow up calls to get more information through structured scripts for each strand. Through this local residents can provide highly detailed information that can be reflected in sophisticated flood protection models and be core to framing urban resilience strategies and optimising the effectiveness of investment. This paper will describe this pioneering approach that will develop flood event data in support of systems that will advance existing approaches such as developed in the in the UK

  3. Efficiency enhancement in solid state dye sensitized solar cells by including inverse opals with controlled layer thicknesses

    Science.gov (United States)

    Zheng, Hanbin; Shah, Said Karim; Abbas, Mamatimin; Ly, Isabelle; Rivera, Thomas; Almeida, Rui M.; Hirsch, Lionel; Toupance, Thierry; Ravaine, Serge

    2016-09-01

    The photoconversion efficiency of dye sensitized solar cells can be enhanced by the incorporation of light management nanostructures such as photonic crystals. Here, we present a facile route to incorporate titania inverse opals into solid state dye sensitized solar cells and report photoconversion efficiency enhancements of up to 56% compared with a model system without the inverse opal. Our approach is based on the precise design of titania inverse opals with a predetermined thickness that can be controlled at the individual layer level. By choosing an inverse opal exhibiting a photonic bandgap which overlaps the absorption bands of the dye, our results show that there is an optimal thickness of the inverse opal structure for maximum efficiency enhancement of the cell. This is the first experimental proof that the thickness of a titania inverse opal plays a pivotal role in cell efficiency enhancement in solid state dye sensitized solar cells.

  4. HIV-1 Nef and Vpu Are Functionally Redundant Broad-Spectrum Modulators of Cell Surface Receptors, Including Tetraspanins

    Science.gov (United States)

    Haller, Claudia; Müller, Birthe; Fritz, Joëlle V.; Lamas-Murua, Miguel; Stolp, Bettina; Pujol, François M.; Keppler, Oliver T.

    2014-01-01

    ABSTRACT HIV-1 Nef and Vpu are thought to optimize virus replication in the infected host, at least in part via their ability to interfere with vesicular host cell trafficking. Despite the use of distinct molecular mechanisms, Nef and Vpu share specificity for some molecules such as CD4 and major histocompatibility complex class I (MHC-I), while disruption of intracellular transport of the host cell restriction factor CD317/tetherin represents a specialized activity of Vpu not exerted by HIV-1 Nef. To establish a profile of host cell receptors whose intracellular transport is affected by Nef, Vpu, or both, we comprehensively analyzed the effect of these accessory viral proteins on cell surface receptor levels on A3.01 T lymphocytes. Thirty-six out of 105 detectable receptors were significantly downregulated by HIV-1 Nef, revealing a previously unappreciated scope with which HIV-1 Nef remodels the cell surface of infected cells. Remarkably, the effects of HIV-1 Vpu on host cell receptor exposure largely matched those of HIV-1 Nef in breadth and specificity (32 of 105, all also targeted by Nef), even though the magnitude was generally less pronounced. Of particular note, cell surface exposure of all members of the tetraspanin (TSPAN) protein family analyzed was reduced by both Nef and Vpu, and the viral proteins triggered the enrichment of TSPANs in a perinuclear area of the cell. While Vpu displayed significant colocalization and physical association with TSPANs, interactions of Nef with TSPANs were less robust. TSPANs thus emerge as a major target of deregulation in host cell vesicular transport by HIV-1 Nef and Vpu. The conservation of this activity in two independent accessory proteins suggests its importance for the spread of HIV-1 in the infected host. IMPORTANCE In this paper, we define that HIV-1 Nef and Vpu display a surprising functional overlap and affect the cell surface exposure of a previously unexpected breadth of cellular receptors. Our analyses

  5. Regulation of the Proliferation of Colon Cancer Cells by Compounds that Affect Glycolysis, Including 3-Bromopyruvate, 2-Deoxyglucose and Biguanides

    OpenAIRE

    Lea, Michael A.; Qureshi, Mehreen S.; Buxhoeveden, Michael; Gengel, Nicolette; Kleinschmit, Jessica; desBordes, Charles

    2013-01-01

    In previous studies we observed that 2-deoxyglucose blocked the acidification of the medium used for culture of colon cancer cells caused by incubation with biguanides and had an additive inhibitory effect on growth. In the present work, we found that 3-bromopyruvate can also prevent the lowering of pH caused by biguanide treatment. 3-Bromopyruvate inhibited colonic cancer cell proliferation but the effect was not always additive to that of biguanides and an additive effect was more notable i...

  6. Detailed balance and entanglement

    International Nuclear Information System (INIS)

    We study a connection between quantum detailed balance, which is a concept of importance in statistical mechanics, and entanglement. We also explore how this connection fits into thermofield dynamics. (paper)

  7. Detailed Soils 24K

    Data.gov (United States)

    Kansas Data Access and Support Center — This data set is a digital soil survey and is the most detailed level of soil geographic data developed by the National Cooperative Soil Survey. The information was...

  8. Degeneration of retinal on bipolar cells induced by serum including autoantibody against TRPM1 in mouse model of paraneoplastic retinopathy.

    Directory of Open Access Journals (Sweden)

    Shinji Ueno

    Full Text Available The paraneoplastic retinopathies (PRs are a group of eye diseases characterized by a sudden and progressive dysfunction of the retina caused by an antibody against a protein in a neoplasm. Evidence has been obtained that the transient receptor potential melastatin 1 (TRPM1 protein was one of the antigens for the autoantibody against the ON bipolar cells in PR patients. However, it has not been determined how the autoantibody causes the dysfunction of the ON bipolar cells. We hypothesized that the antibody against TRPM1 in the serum of patients with PR causes a degeneration of retinal ON bipolar cells. To test this hypothesis, we injected the serum from the PR patient, previously shown to contain anti-TRPM1 antibodies by westerblot, intravitreally into mice and examined the effects on the retina. We found that the electroretinograms (ERGs of the mice were altered acutely after the injection, and the shape of the ERGs resembled that of the patient with PR. Immunohistochemical analysis of the eyes injected with the serum showed immunoreactivity against bipolar cells only in wild-type animals and not in TRPM1 knockout mice,consistent with the serum containing anti-TRPM1 antibodies. Histology also showed that some of the bipolar cells were apoptotic by 5 hours after the injection in wild type mice, but no bipolar cell death was found in TRPM1 knockout mice, . At 3 months, the inner nuclear layer was thinner and the amplitudes of the ERGs were still reduced. These results indicate that the serum of a patient with PR contained an antibody against TRPM1 caused an acute death of retinal ON bipolar cells of mice.

  9. Fuel cell integral bundle assembly including ceramic open end seal and vertical and horizontal thermal expansion control

    Science.gov (United States)

    Zafred, Paolo R.; Gillett, James E.

    2012-04-24

    A plurality of integral bundle assemblies contain a top portion with an inlet fuel plenum and a bottom portion containing a base support, the base supports a dense, ceramic air exhaust manifold having four supporting legs, the manifold is below and connects to air feed tubes located in a recuperator zone, the air feed tubes passing into the center of inverted, tubular, elongated, hollow electrically connected solid oxide fuel cells having an open end above a combustion zone into which the air feed tubes pass and a closed end near the inlet fuel plenum, where the open end of the fuel cells rest upon and within a separate combination ceramic seal and bundle support contained in a ceramic support casting, where at least one flexible cushion ceramic band seal located between the recuperator and fuel cells protects and controls horizontal thermal expansion, and where the fuel cells operate in the fuel cell mode and where the base support and bottom ceramic air exhaust manifolds carry from 85% to all of the weight of the generator.

  10. Regulation of the proliferation of colon cancer cells by compounds that affect glycolysis, including 3-bromopyruvate, 2-deoxyglucose and biguanides.

    Science.gov (United States)

    Lea, Michael A; Qureshi, Mehreen S; Buxhoeveden, Michael; Gengel, Nicolette; Kleinschmit, Jessica; Desbordes, Charles

    2013-02-01

    In previous studies performed by our group, we observed that 2-deoxyglucose blocked the acidification of the medium used for culture of colon cancer cells caused by incubation with biguanides and it had an additive inhibitory effect on growth. In the present work, we found that 3-bromopyruvate can also prevent the lowering of pH caused by biguanide treatment. 3-Bromopyruvate inhibited colonic cancer cell proliferation, but the effect was not always additive to that of biguanides and an additive effect was more notable in combined treatment with 3-bromopyruvate and 2-deoxyglucose. The induction of alkaline phosphatase activity by butyrate was not consistently affected by combination with other agents that modified glucose metabolism. The drug combinations that were examined inhibited proliferation of wild-type and p53-null cells and affected colonic cancer lines with different growth rates. PMID:23393330

  11. T cell determinants of myelin basic protein include a unique encephalitogenic I-E-restricted epitope for Lewis rats

    OpenAIRE

    1989-01-01

    The major encephalitogenic epitope for Lewis rats is the 72-89 sequence of guinea pig basic protein (GP-BP) or rat basic protein (Rt-BP). T cells responsive to this epitope are I-A restricted and preferentially express the V alpha 2:V beta 8 gene combination in their TCR. In this work, we describe for the first time the delayed appearance of T cells specific for additional discrete determinant of BP, the nonencephalitogenic 55-68 sequence of GP-BP restricted by I-A, and the encephalitogenic 8...

  12. The conjectured S-type retrograde planet in ν Octantis: more evidence including four years of iodine-cell radial velocities

    Science.gov (United States)

    Ramm, D. J.; Nelson, B. E.; Endl, M.; Hearnshaw, J. B.; Wittenmyer, R. A.; Gunn, F.; Bergmann, C.; Kilmartin, P.; Brogt, E.

    2016-08-01

    We report 1212 radial-velocity (RV) measurements obtained in the years 2009-2013 using an iodine cell for the spectroscopic binary ν Octantis (K1 III/IV). This system (a_{bin} ˜ 2.6 au, P ˜ 1050 d) is conjectured to have a Jovian planet with a semimajor axis half that of the binary host. The extreme geometry only permits long-term stability if the planet is in a retrograde orbit. Whilst the reality of the planet (P ˜ 415 d) remains uncertain, other scenarios (stellar variability or apsidal motion caused by a yet unobserved third star) continue to appear substantially less credible based on cross-correlation function bisectors, line-depth ratios and many other independent details. If this evidence is validated but the planet is disproved, the claims of other planets using RVs will be seriously challenged. We also describe a significant revision to the previously published RVs and the full set of 1437 RVs now encompasses nearly 13 yr. The sensitive orbital dynamics allow us to constrain the 3D architecture with a broad prior probability distribution on the mutual inclination, which with posterior samples obtained from an N-body Markov chain Monte Carlo is found to be 152.5°±^{0.7}_{0.6}. None of these samples are dynamically stable beyond 106 yr. However, a grid search around the best-fitting solution finds a region that has many models stable for 107 yr, and includes one model within 1σ that is stable for at least 108 yr. The planet's exceptional nature demands robust independent verification and makes the theoretical understanding of its formation a worthy challenge.

  13. The conjectured S-type retrograde planet in ν Octantis: more evidence including four years of iodine-cell radial velocities

    Science.gov (United States)

    Ramm, D. J.; Nelson, B. E.; Endl, M.; Hearnshaw, J. B.; Wittenmyer, R. A.; Gunn, F.; Bergmann, C.; Kilmartin, P.; Brogt, E.

    2016-08-01

    We report 1212 radial-velocity (RV) measurements obtained in the years 2009-2013 using an iodine cell for the spectroscopic binary nu Octantis (K1III/IV). This system (a_bin~2.6 au, P~1050 days) is conjectured to have a Jovian planet with a semi-major axis half that of the binary host. The extreme geometry only permits long-term stability if the planet is in a retrograde orbit. Whilst the reality of the planet (P~415 days) remains uncertain, other scenarios (stellar variability or apsidal motion caused by a yet unobserved third star) continue to appear substantially less credible based on CCF bisectors, line-depth ratios and many other independent details. If this evidence is validated but the planet is disproved, the claims of other planets using RVs will be seriously challenged. We also describe a significant revision to the previously published RVs and the full set of 1437 RVs now encompasses nearly 13 years. The sensitive orbital dynamics allow us to constrain the three-dimensional architecture with a broad prior probability distribution on the mutual inclination, which with posterior samples obtained from an N-body Markov chain Monte Carlo is found to be 158.4 +/- 1.2 deg. None of these samples are dynamically stable beyond 1 Myr. However, a grid search around the best-fitting solution finds a region that has many models stable for 10 Myr, and includes one model within 1-sigma that is stable for at least 100 Myr. The planet's exceptional nature demands robust independent verification and makes the theoretical understanding of its formation a worthy challenge.

  14. Non-islet cell tumour-induced hypoglycaemia: a review of the literature including two new cases.

    NARCIS (Netherlands)

    Groot, J.W. de; Rikhof, B.; Doorn, J. van; Bilo, H.J.; Alleman, M.A.; Honkoop, A.H.; Graaf, W.T.A. van der

    2007-01-01

    This review focuses on the tumour types and symptoms associated with non-islet cell tumour-induced hypoglycaemia (NICTH) as well as the pathogenesis, diagnosis and treatment of this rare paraneoplastic phenomenon. In addition, we report two illustrative cases of patients suffering from NICTH caused

  15. Non-islet cell tumour-induced hypoglycaemia : a review of the literature including two new cases

    NARCIS (Netherlands)

    De Groot, Jan Willem B.; Rikhof, Bart; Van Doom, Jaap; Bilo, Henk J. G.; Alleman, Maarten A.; Honkoop, Aafke H.; Van der Graaf, Winette T. A.

    2007-01-01

    This review focuses on the tumour types and symptoms associated with non-islet cell tumour-induced hypoglycaemia (NICTH) as well as the pathogenesis, diagnosis and treatment of this rare paraneoplastic phenomenon. In addition, we report two illustrative cases of patients suffering from NICTH caused

  16. 2', 3'-cyclic nucleotide 3'-phosphodiesterase is expressed in dissociated rat cerebellar cells and included in the postsynaptic density fraction.

    Science.gov (United States)

    Cho, Sun-Jung; Jung, Jae Seob; Jin, IngNyol; Moon, Il Soo

    2003-08-31

    We have shown by protein sequencing that the phosphotyrosine-containing 48 kDa protein band of the rat cerebellar postsynaptic density fraction (CBL-PSD) is 2', 3'-cyclic nucleotide 3'-phosphodiesterase 2 (CNP2). Immunoblot analysis indicated that both CNP1 and CNP2 isoforms are present in the CBL-PSD fraction, whereas there is little CNP2 in the forebrain (FB)-PSD fraction. Both isoforms in the CBL-PSD fraction were tyrosine-phosphorylated to a basal extent. They were efficiently dissociated from the complexes in the PSD fraction by salt, but not by non-ionic detergents such as n-octyl glucoside (OG) and Triton X-100. Immunocytochemistry of dissociated cerebellar cultures revealed patchy CNP staining in oligodendrocytes (OLs), Purkinje cells (PCs), and unidentified PSD95-positive cells, but no staining in granule cells (GCs). Our results indicate that both CNP1 and CNP2 are expressed in cerian populations of cerebellar cells in addition to OL, and that they are associated with complexes that are co-isolated with the PSD. PMID:14503857

  17. Removal of an oral squamous cell carcinoma including parts of osseointegrated implants in the marginal mandibulectomy. A case report.

    NARCIS (Netherlands)

    Meijer, G.J.; Dieleman, F.J.; Berge, S.J.; Merkx, M.A.W.

    2010-01-01

    PURPOSE: The incidence of oral squamous cell carcinomas (OSCC) arising around dental implants will increase because of the rising popularity of dental implants. In this case, a novel surgical treatment of an OSCC in the vicinity of endosseous implants is reported. MATERIALS AND METHODS: In a 69-year

  18. The HER2 amplicon includes several genes required for the growth and survival of HER2 positive breast cancer cells — A data description

    Directory of Open Access Journals (Sweden)

    Vesa Hongisto

    2014-12-01

    Full Text Available A large number of breast cancers are characterized by amplification and overexpression of the chromosome segment surrounding the HER2 (ERBB2 oncogene. As the HER2 amplicon at 17q12 contains multiple genes, we have systematically explored the role of the HER2 co-amplified genes in breast cancer cell growth and their relation to trastuzumab resistance. We integrated array comparative genomic hybridization (aCGH data of the HER2 amplicon from 71 HER2 positive breast tumors and 10 cell lines with systematic functional RNA interference analysis of 23 core amplicon genes with several phenotypic endpoints in a panel of trastuzumab responding and non-responding HER2 positive breast cancer cells. In this Data in Brief we give a detailed description of the experimental procedures and the data analysis methods used in the study (1.

  19. Removal of an oral squamous cell carcinoma including parts of osseointegrated implants in the marginal mandibulectomy. A case report

    OpenAIRE

    Meijer, Gert J; Dieleman, Francois J.; Stefaan J Bergé; Merkx, Matthias A.W.

    2010-01-01

    Purpose The incidence of oral squamous cell carcinomas (OSCC) arising around dental implants will increase because of the rising popularity of dental implants. In this case, a novel surgical treatment of an OSCC in the vicinity of endosseous implants is reported. Materials and methods In a 69-year-old woman, a recurrent OSCC (cT2N0M0) developed in the floor of the mouth extending to the attached keratinized peri-implant mucosa of both interforaminal-placed dental implants. Radiographically, n...

  20. A Molecular Profile of Cocaine Abuse Includes the Differential Expression of Genes that Regulate Transcription, Chromatin, and Dopamine Cell Phenotype

    Science.gov (United States)

    Bannon, Michael J; Johnson, Magen M; Michelhaugh, Sharon K; Hartley, Zachary J; Halter, Steven D; David, James A; Kapatos, Gregory; Schmidt, Carl J

    2014-01-01

    Chronic drug abuse, craving, and relapse are thought to be linked to long-lasting changes in neural gene expression arising through transcriptional and chromatin-related mechanisms. The key contributions of midbrain dopamine (DA)-synthesizing neurons throughout the addiction process provide a compelling rationale for determining the drug-induced molecular changes that occur in these cells. Yet our understanding of these processes remains rudimentary. The postmortem human brain constitutes a unique resource that can be exploited to gain insights into the pathophysiology of complex disorders such as drug addiction. In this study, we analyzed the profiles of midbrain gene expression in chronic cocaine abusers and well-matched drug-free control subjects using microarray and quantitative PCR. A small number of genes exhibited robust differential expression; many of these are involved in the regulation of transcription, chromatin, or DA cell phenotype. Transcript abundances for approximately half of these differentially expressed genes were diagnostic for assigning subjects to the cocaine-abusing vs control cohort. Identification of a molecular signature associated with pathophysiological changes occurring in cocaine abusers' midbrains should contribute to the development of biomarkers and novel therapeutic targets for drug addiction. PMID:24642598

  1. A constitutive model for the compressive response of metallic closed-cell foams including micro-inertia effects

    Directory of Open Access Journals (Sweden)

    Barthélémy Romain

    2015-01-01

    Full Text Available Metallic foams have known a keen interest in the last decades. Their ability to undergo very large deformations while transmitting low stress levels make them capable of performing functions of protective layers against intense loadings and of energy absorbers, for instance. The behaviour of metal foams varies considerably between quasi-static and dynamic regimes. Those differences can be linked to the strain-rate sensitivity of the skeleton material and to micro-inertial effects (induced by the crushing of the foam cells. In the present work, a micromechanical model has been developed to take into account micro-inertia effects on the macroscopic behaviour of closed-cell foams under dynamic loading conditions. The proposed modelling is based on the dynamic homogenisation procedure introduced by Molinari and Mercier (J. Mech. Phys. Solids 49 (2001 1497–1516. Within this framework, the macrostress is the sum of two terms. The first one is a static stress, that can be described with any existing model of metal foam. The second contribution is a dynamic stress related to micro-inertia effects. Considering an initially spherical shell as a Representative Volume Element (RVE of the foam material, a closed-form expression of the dynamic stress was obtained. The proposed modelling was applied to shock propagation in aluminium foams (it should however be noted that the present theory is not restricted to uniaxial deformation but can be applied to arbitrary loadings. From experimental data of the literature, it is observed that incorporating micro-inertia effects allows one to achieve a better description of the foam shock response. This indicates that micro-inertia may have a significant influence on the dynamic behaviour of metallic foams.

  2. Detailed impedance characterization of a well performing and durable Ni:CGO infiltrated cermet anode for metal-supported solid oxide fuel cells

    DEFF Research Database (Denmark)

    Nielsen, Jimmi; Klemensø, Trine; Blennow Tullmar, Peter

    2012-01-01

    as a transmission line response could account for the intermediate frequency arc. The PET model enabled a detailed insight into the effect of adding minor amounts of Ni into the infiltrated CGO and allowed an estimation of important characteristics such as the electrochemical utilization thickness of the anode...

  3. Three Latin Phonological Details

    DEFF Research Database (Denmark)

    Olsen, Birgit Anette

    2006-01-01

    The present paper deals with three minor details of Latin phonology: 1) the development of the initial sequence *u¿l¿-, where it is suggested that an apparent vacillation between ul- and vol-/vul- represents sandhi variants going back to the proto-language, 2) the adjectives ama¯rus ‘bitter' and ......' and ava¯rus ‘greedy' and 3) the internal cluster -mpl- of the nouns exemplum and templum....

  4. Primary T-cells from human CD4/CCR5-transgenic rats support all early steps of HIV-1 replication including integration, but display impaired viral gene expression

    Directory of Open Access Journals (Sweden)

    Hermann Volker

    2007-07-01

    cells complete all of the early steps in the HIV-1 replication cycle, including provirus integration in vivo, with high efficiency. A deficiency in gene expression was disclosed at the single cell level and could be counteracted by the human pTEFb transcription complex factor Cyclin T1. Collectively, these results provide the basis for the advancement of this transgenic rat model through strategies aimed at boosting HIV-1 gene expression in primary rat CD4 T-cells, including human Cyclin T1 transgenesis.

  5. PREFACE: 9th International Fröhlich's Symposium: Electrodynamic Activity of Living Cells (Including Microtubule Coherent Modes and Cancer Cell Physics)

    Science.gov (United States)

    Cifra, Michal; Pokorný, Jirí; Kucera, Ondrej

    2011-12-01

    This volume contains papers presented at the International Fröhlich's Symposium entitled 'Electrodynamic Activity of Living Cells' (1-3 July 2011, Prague, Czech Republic). The Symposium was the 9th meeting devoted to physical processes in living matter organized in Prague since 1987. The hypothesis of oscillation systems in living cells featured by non-linear interaction between elastic and electrical polarization fields, non-linear interactions between the system and the heat bath leading to energy downconversion along the frequency scale, energy condensation in the lowest frequency mode and creation of a coherent state was formulated by H Fröhlich, founder of the theory of dielectric materials. He assumed that biological activity is based not only on biochemical but also on biophysical mechanisms and that their disturbances form basic links along the cancer transformation pathway. Fröhlich outlined general ideas of non-linear physical processes in biological systems. The downconversion and the elastic-polarization interactions should be connected in a unified theory and the solution based on comprehensive non-linear characteristics. Biochemical and genetic research of biological systems are highly developed and have disclosed a variety of cellular and subcellular structures, chemical reactions, molecular information transfer, and genetic code sequences - including their pathological development. Nevertheless, the cancer problem is still a big challenge. Warburg's discovery of suppressed oxidative metabolism in mitochondria in cancer cells suggested the essential role of physical mechanisms (but his discovery has remained without impact on cancer research and on the study of physical properties of biological systems for a long time). Mitochondria, the power plants of the cell, have several areas of activity-oxidative energy production is connected with the formation of a strong static electric field around them, water ordering, and liberation of non

  6. GLI1 confers profound phenotypic changes upon LNCaP prostate cancer cells that include the acquisition of a hormone independent state.

    Directory of Open Access Journals (Sweden)

    Sandeep K Nadendla

    Full Text Available The GLI (GLI1/GLI2 transcription factors have been implicated in the development and progression of prostate cancer although our understanding of how they actually contribute to the biology of these common tumours is limited. We observed that GLI reporter activity was higher in normal (PNT-2 and tumourigenic (DU145 and PC-3 androgen-independent cells compared to androgen-dependent LNCaP prostate cancer cells and, accordingly, GLI mRNA levels were also elevated. Ectopic expression of GLI1 or the constitutively active ΔNGLI2 mutant induced a distinct cobblestone-like morphology in LNCaP cells that, regarding the former, correlated with increased GLI2 as well as expression of the basal/stem-like markers CD44, β1-integrin, ΔNp63 and BMI1, and decreased expression of the luminal marker AR (androgen receptor. LNCaP-GLI1 cells were viable in the presence of the AR inhibitor bicalutamide and gene expression profiling revealed that the transcriptome of LNCaP-GLI1 cells was significantly closer to DU145 and PC-3 cells than to control LNCaP-pBP (empty vector cells, as well as identifying LCN2/NGAL as a highly induced transcript which is associated with hormone independence in breast and prostate cancer. Functionally, LNCaP-GLI1 cells displayed greater clonal growth and were more invasive than control cells but they did not form colonies in soft agar or prostaspheres in suspension suggesting that they do not possess inherent stem cell properties. Moreover, targeted suppression of GLI1 or GLI2 with siRNA did not reverse the transformed phenotype of LNCaP-GLI1 cells nor did double GLI1/GLI2 knockdowns activate AR expression in DU145 or PC-3 cells. As such, early targeting of the GLI oncoproteins may hinder progression to a hormone independent state but a more detailed understanding of the mechanisms that maintain this phenotype is required to determine if their inhibition will enhance the efficacy of anti-hormonal therapy through the induction of a luminal

  7. A recurrent germline BAP1 mutation and extension of the BAP1 tumor predisposition spectrum to include basal cell carcinoma

    DEFF Research Database (Denmark)

    Wadt, Karin Anna Wallentin; Aoude, L G; Johansson, P;

    2015-01-01

    We report four previously undescribed families with germline BRCA1-associated protein-1 gene (BAP1) mutations and expand the clinical phenotype of this tumor syndrome. The tumor spectrum in these families is predominantly uveal malignant melanoma (UMM), cutaneous malignant melanoma (CMM) and meso......We report four previously undescribed families with germline BRCA1-associated protein-1 gene (BAP1) mutations and expand the clinical phenotype of this tumor syndrome. The tumor spectrum in these families is predominantly uveal malignant melanoma (UMM), cutaneous malignant melanoma (CMM......) and mesothelioma, as previously reported for germline BAP1 mutations. However, mutation carriers from three new families, and one previously reported family, developed basal cell carcinoma (BCC), thus suggesting inclusion of BCC in the phenotypic spectrum of the BAP1 tumor syndrome. This notion is supported...... by the finding of loss of BAP1 protein expression by immunochemistry in two BCCs from individuals with germline BAP1 mutations and no loss of BAP1 staining in 53 of sporadic BCCs consistent with somatic mutations and loss of heterozygosity of the gene in the BCCs occurring in mutation carriers. Lastly, we...

  8. Pleural fluid cell-free DNA integrity index to identify cytologically negative malignant pleural effusions including mesotheliomas

    Directory of Open Access Journals (Sweden)

    Sriram Krishna B

    2012-09-01

    Full Text Available Abstract Background The diagnosis of malignant pleural effusions (MPE is often clinically challenging, especially if the cytology is negative for malignancy. DNA integrity index has been reported to be a marker of malignancy. The aim of this study was to evaluate the utility of pleural fluid DNA integrity index in the diagnosis of MPE. Methods We studied 75 pleural fluid and matched serum samples from consecutive subjects. Pleural fluid and serum ALU DNA repeats [115bp, 247bp and 247bp/115bp ratio (DNA integrity index] were assessed by real-time quantitative PCR. Pleural fluid and serum mesothelin levels were quantified using ELISA. Results Based on clinico-pathological evaluation, 52 subjects had MPE (including 16 mesotheliomas and 23 had benign effusions. Pleural fluid DNA integrity index was higher in MPE compared with benign effusions (1.2 vs. 0.8; p Conclusion Pleural fluid DNA integrity index is a promising diagnostic biomarker for identification of MPEs, including mesothelioma. This biomarker may be particularly useful in cases of MPE where pleural aspirate cytology is negative, and could guide the decision to undertake more invasive definitive testing. A prospective validation study is being undertaken to validate our findings and test the clinical utility of this biomarker for altering clinical practice.

  9. Expression patterns of DLK1 and INSL3 identify stages of Leydig cell differentiation during normal development and in testicular pathologies, including testicular cancer and Klinefelter syndrome

    DEFF Research Database (Denmark)

    Lottrup, G; Nielsen, J E; Maroun, L L;

    2014-01-01

    STUDY QUESTION: What is the differentiation stage of human testicular interstitial cells, in particular Leydig cells (LC), within micronodules found in patients with infertility, testicular cancer and Klinefelter syndrome? SUMMARY ANSWER: The Leydig- and peritubular-cell populations in testes with......, are impaired in adult men with testicular pathologies including testis cancer and Klinefelter syndrome. STUDY FUNDING/COMPETING INTERESTS: This work was funded by Rigshospitalet's research funds, the Danish Cancer Society and Kirsten and Freddy Johansen's foundation. The authors have no conflicts of...... specimens and in 58 adult testis samples from patients with testicular germ cell tumours, including precursor carcinoma in situ (CIS), infertility or Klinefelter syndrome. PARTICIPANTS/MATERIALS, SETTING, METHODS: The expression patterns of DLK1, INSL3, chicken ovalbumin upstream promoter transcription...

  10. Fuel cell system including a unit for electrical isolation of a fuel cell stack from a manifold assembly and method therefor

    Science.gov (United States)

    Kelley; Dana A. , Farooque; Mohammad , Davis; Keith

    2007-10-02

    A fuel cell system with improved electrical isolation having a fuel cell stack with a positive potential end and a negative potential, a manifold for use in coupling gases to and from a face of the fuel cell stack, an electrical isolating assembly for electrically isolating the manifold from the stack, and a unit for adjusting an electrical potential of the manifold such as to impede the flow of electrolyte from the stack across the isolating assembly.

  11. R5-SHIV induces multiple defects in T cell function during early infection of rhesus macaques including accumulation of T reg cells in lymph nodes.

    Directory of Open Access Journals (Sweden)

    Michael Santosuosso

    Full Text Available BACKGROUND: HIV-1 is a pathogen that T cell responses fail to control. HIV-1gp120 is the surface viral envelope glycoprotein that interacts with CD4 T cells and mediates entry. HIV-1gp120 has been implicated in immune dysregulatory functions that may limit anti-HIV antigen-specific T cell responses. We hypothesized that in the context of early SHIV infection, immune dysregulation of antigen-specific T-effector cell and regulatory functions would be detectable and that these would be associated or correlated with measurable concentrations of HIV-1gp120 in lymphoid tissues. METHODS: Rhesus macaques were intravaginally inoculated with a Clade C CCR5-tropic simian-human immunodeficiency virus, SHIV-1157ipd3N4. HIV-1gp120 levels, antigen-specificity, levels of apoptosis/anergy and frequency and function of Tregs were examined in lymph node and blood derived T cells at 5 and 12 weeks post inoculation. RESULTS/CONCLUSIONS: We observed reduced responses to Gag in CD4 and gp120 in CD8 lymph node-derived T cells compared to the peripheral blood at 5 weeks post-inoculation. Reduced antigen-specific responses were associated with higher levels of PD-1 on lymph node-derived CD4 T cells as compared to peripheral blood and uninfected lymph node-derived CD4 T cells. Lymph nodes contained increased numbers of Tregs as compared to peripheral blood, which positively correlated with gp120 levels; T regulatory cell depletion restored CD8 T cell responses to Gag but not to gp120. HIV gp120 was also able to induce T regulatory cell chemotaxis in a dose-dependent, CCR5-mediated manner. These studies contribute to our broader understanding of the ways in which HIV-1 dysregulates T cell function and localization during early infection.

  12. Pleural fluid cell-free DNA integrity index to identify cytologically negative malignant pleural effusions including mesotheliomas

    International Nuclear Information System (INIS)

    The diagnosis of malignant pleural effusions (MPE) is often clinically challenging, especially if the cytology is negative for malignancy. DNA integrity index has been reported to be a marker of malignancy. The aim of this study was to evaluate the utility of pleural fluid DNA integrity index in the diagnosis of MPE. We studied 75 pleural fluid and matched serum samples from consecutive subjects. Pleural fluid and serum ALU DNA repeats [115bp, 247bp and 247bp/115bp ratio (DNA integrity index)] were assessed by real-time quantitative PCR. Pleural fluid and serum mesothelin levels were quantified using ELISA. Based on clinico-pathological evaluation, 52 subjects had MPE (including 16 mesotheliomas) and 23 had benign effusions. Pleural fluid DNA integrity index was higher in MPE compared with benign effusions (1.2 vs. 0.8; p<0.001). Cytology had a sensitivity of 55% in diagnosing MPE. If cytology and pleural fluid DNA integrity index were considered together, they exhibited 81% sensitivity and 87% specificity in distinguishing benign and malignant effusions. In cytology-negative pleural effusions (35 MPE and 28 benign effusions), elevated pleural fluid DNA integrity index had an 81% positive predictive value in detecting MPEs. In the detection of mesothelioma, at a specificity of 90%, pleural fluid DNA integrity index had similar sensitivity to pleural fluid and serum mesothelin (75% each respectively). Pleural fluid DNA integrity index is a promising diagnostic biomarker for identification of MPEs, including mesothelioma. This biomarker may be particularly useful in cases of MPE where pleural aspirate cytology is negative, and could guide the decision to undertake more invasive definitive testing. A prospective validation study is being undertaken to validate our findings and test the clinical utility of this biomarker for altering clinical practice

  13. Detailed Debunking of Denial

    Science.gov (United States)

    Enting, I. G.; Abraham, J. P.

    2012-12-01

    The disinformation campaign against climate science has been compared to a guerilla war whose tactics undermine the traditional checks and balances of science. One comprehensive approach has to been produce archives of generic responses such as the websites of RealClimate and SkepticalScience. We review our experiences with an alternative approach of detailed responses to a small number of high profile cases. Our particular examples were Professor Ian Plimer and Christopher Monckton, the Third Viscount Monckton of Brenchley, each of whom has been taken seriously by political leaders in our respective countries. We relate our experiences to comparable examples such as John Mashey's analysis of the Wegman report and the formal complaints about Lomborg's "Skeptical Environmentalist" and Durkin's "Great Global Warming Swindle". Our two approaches used contrasting approaches: an on-line video of a lecture vs an evolving compendium of misrepresentations. Additionally our approaches differed in the emphasis. The analysis of Monckton concentrated on the misrepresentation of the science, while the analysis of Plimer concentrated on departures from accepted scientific practice: fabrication of data, misrepresentation of cited sources and unattributed use of the work of others. Benefits of an evolving compendium were the ability to incorporate contributions from members of the public who had identified additional errors and the scope for addressing new aspects as they came to public attention. `Detailed debunking' gives non-specialists a reference point for distinguishing non-science when engaging in public debate.

  14. Charybdotoxin is a new member of the K+ channel toxin family that includes dendrotoxin I and mast cell degranulating peptide

    International Nuclear Information System (INIS)

    A polypeptide was identified in the venom of the scorpion Leiurus quinquestriatus hebraeus by its potency to inhibit the high affinity binding of the radiolabeled snake venom toxin dendrotoxin I (125I-DTXI) to its receptor site. It has been purified, and its properties investigated by different techniques were found to be similar to those of MCD and DTXI, two polypeptide toxins active on a voltage-dependent K+ channel. However, its amino acid sequence was determined, and it was shown that this toxin is in fact charybdotoxin (ChTX), a toxin classically used as a specific tool to block one class of Ca2+-activated K+ channels. ChTX, DTXI, and MCD are potent convulsants and are highly toxic when injected intracerebroventricularly in mice. Their toxicities correlate well with their affinities for their receptors in rat brain. These three structurally different toxins release [3H]GABA from preloaded synaptosomes, the efficiency order being DTXI > ChTX > MCD. Both binding and cross-linking experiments of ChTX to rat brain membranes and to the purified MCD/DTXI binding protein have shown that the α-subunit of the MCD/DTXI-sensitive K+ channel protein also contains the ChTX binding sites. Binding sites for DTXI, MCD, and ChTX are in negative allosteric interaction. The results show that charybdotoxin belongs to the family of toxins which already includes the dendrotoxins and MCD, which are blockers of voltage-sensitive K+ channels. ChTX is clearly not selective for Ca2+-activated K+ channel

  15. The ethanol extract of Scutellaria baicalensis and the active compounds induce cell cycle arrest and apoptosis including upregulation of p53 and Bax in human lung cancer cells

    International Nuclear Information System (INIS)

    Despite a lack of scientific authentication, Scutellaria baicalensis is clinically used in Chinese medicine as a traditional adjuvant to chemotherapy of lung cancer. In this study, cytotoxicity assays demonstrated that crude ethanolic extracts of S. baicalensis were selectively toxic to human lung cancer cell lines A549, SK-LU-1 and SK-MES-1 compared with normal human lung fibroblasts. The active compounds baicalin, baicalein and wogonin did not exhibit such selectivity. Following exposure to the crude extracts, cellular protein expression in the cancer cell lines was assessed using 2D gel electrophoresis coupled with MALDI-TOF-MS/Protein Fingerprinting. The altered protein expression indicated that cell growth arrest and apoptosis were potential mechanisms of cytotoxicity. These observations were supported by PI staining cell cycle analysis using flow cytometry and Annexin-V apoptotic analysis by fluorescence microscopy of cancer cells treated with the crude extract and pure active compounds. Moreover, specific immunoblotting identification showed the decreased expression of cyclin A results in the S phase arrest of A549 whereas the G0/G1 phase arrest in SK-MES-1 cells results from the decreased expression of cyclin D1. Following treatment, increased expression in the cancer cells of key proteins related to the enhancement of apoptosis was observed for p53 and Bax. These results provide further insight into the molecular mechanisms underlying the clinical use of this herb as an adjuvant to lung cancer therapy. - Research highlights: → Scutellaria baicalensis is a clinical adjuvant to lung cancer chemotherapy in China. → Scutellaria ethanol extracts selectively toxic to A549, SK-LU-1 and SK-MES-1. → Baicalin, baicalein and wogonin were toxic to all lung cancer cell lines. → Proteomics identified increased p53 and BAX in response to Scutellaria extracts.

  16. Bcl-1 gene rearrangements in mantle cell lymphoma : A comprehensive analysis of 118 cases, including B-5-fixed tissue, by polymerase chain reaction and southern transfer analysis

    NARCIS (Netherlands)

    Chibbar, R; Leung, K; McCormick, S; Ritzkalla, K; Strickler, J; Staggs, R; Poppema, S; Brunning, RD; McGlennen, RC

    1998-01-01

    We evaluated 118 cases of mantle cell lymphoma by polymerase chain reaction (PCR) for the major translocation cluster (MIG) region and another breakpoint corresponding to probe p94(PS), located 24 kb telomeric to the MTC locus on chromosome 11. The specimens included 64 frozen, 19 formalin-fixed, an

  17. Clinicopathological features of aggressive B-cell lymphomas including B-cell lymphoma, unclassifiable, with features intermediate between diffuse large B-cell and Burkitt lymphomas: a study of 44 patients from Argentina.

    Science.gov (United States)

    Bürgesser, María Virginia; Gualco, Gabriela; Diller, Ana; Natkunam, Yasodha; Bacchi, Carlos E

    2013-06-01

    Aggressive B-cell lymphomas incorporate a wide spectrum of lymphomas that pose challenges in diagnosis as well as treatment. We evaluated the clinicopathological features of 44 patients with aggressive B-cell lymphomas which were classified into 3 groups based on the World Health Organization 2008 classification as follows: including 30 cases of diffuse large B-cell lymphoma (DLBCL), 8 cases of Burkitt lymphoma (BL) and 6 cases of B-cell lymphoma, unclassifiable, with features intermediate between Burkitt lymphoma and diffuse large B-cell lymphoma (BCLU). Male predominance was observed in BL and BCLU groups and the mean age varied from 29 years in BL, 61 years in DLBCL and 70 years in BCLU. Patients with BCLU presented at more advanced stages and had a higher international prognostic index. By immunohistochemistry, they shared characteristics of both BL (including more frequent expression of SOX11) and DLBCL. FISH analyses showed three cases with more than one rearrangement: one MYC/BCL2 and two BCL2/BCL6, in addition to which one case with BCL2/IGH translocation and another with MYC rearrangement were also detected. The mean follow-up survival time of BCLU was 6.6 months, which was significantly shorter in comparison to DLBCL (31 months) and BL (30 months), respectively. The importance of recognizing this BCLU group relies on its different clinical course, poor prognosis and shorter survival than DLBCL and BL. An accurate diagnosis is critical for risk stratification and to improve therapeutic approaches and outcomes.

  18. Anti cancer activity on Graviola, an exciting medicinal plant extract vs various cancer cell lines and a detailed computational study on its potent anti-cancerous leads.

    Science.gov (United States)

    Paul, Jeno; Gnanam, R; Jayadeepa, R M; Arul, L

    2013-01-01

    Nature is the world's best chemist: Many naturally occurring compounds have very complicated structures that present great challenges to chemists wishing to determine their structures or replicate them. The plant derived herbal compounds have a long history of clinical use, better patient tolerance and acceptance. Their high ligand binding affinity to the target introduce the prospect of their use in chemo preventive applications; in addition they are freely available natural compounds that can be safely used to prevent various ailments. Plants became the basis of traditional medicine system throughout the world for thousands of years and continue to provide mankind with new remedies. Here, we present a research study on a medicinal plant, Graviola, a native of North America but rarely grown in India. It has a wide potent anticancerous agents coined as Acetogenins which play a key role towards many varieties of cancer, Acetogenins are potent inhibitors of NADH oxidase of the plasma membranes of cancer cells. Potent leads were taken for the study through literature survey, major types of cancer targets were identified, the natureceuticals and the cancer protein were subjected to docking analysis, further with the help of the dock score and other descriptor properties top ranked molecules were collected, commercial drug was also selected and identified as a Test compound for the study. Later, the phytochemicals were subjected to toxicity analysis. Those screened compounds were then considered for active site analysis and to find the best binding site for the study. R Programming library was used to find the best leads. Phytochemicals such as Anonaine, Friedelin, Isolaureline, Annonamine, Anomurine, Kaempferol, Asimilobine, Quercetin, Xylopine were clustered and the highly clustered compounds such as Annonamine , Kaempferol termed to be a potential lead for the study. Further study on experimental analysis may prove the potentiality of these compounds. In the

  19. Enteric bacterial metabolites propionic and butyric acid modulate gene expression, including CREB-dependent catecholaminergic neurotransmission, in PC12 cells--possible relevance to autism spectrum disorders.

    Directory of Open Access Journals (Sweden)

    Bistra B Nankova

    Full Text Available Alterations in gut microbiome composition have an emerging role in health and disease including brain function and behavior. Short chain fatty acids (SCFA like propionic (PPA, and butyric acid (BA, which are present in diet and are fermentation products of many gastrointestinal bacteria, are showing increasing importance in host health, but also may be environmental contributors in neurodevelopmental disorders including autism spectrum disorders (ASD. Further to this we have shown SCFA administration to rodents over a variety of routes (intracerebroventricular, subcutaneous, intraperitoneal or developmental time periods can elicit behavioral, electrophysiological, neuropathological and biochemical effects consistent with findings in ASD patients. SCFA are capable of altering host gene expression, partly due to their histone deacetylase inhibitor activity. We have previously shown BA can regulate tyrosine hydroxylase (TH mRNA levels in a PC12 cell model. Since monoamine concentration is known to be elevated in the brain and blood of ASD patients and in many ASD animal models, we hypothesized that SCFA may directly influence brain monoaminergic pathways. When PC12 cells were transiently transfected with plasmids having a luciferase reporter gene under the control of the TH promoter, PPA was found to induce reporter gene activity over a wide concentration range. CREB transcription factor(s was necessary for the transcriptional activation of TH gene by PPA. At lower concentrations PPA also caused accumulation of TH mRNA and protein, indicative of increased cell capacity to produce catecholamines. PPA and BA induced broad alterations in gene expression including neurotransmitter systems, neuronal cell adhesion molecules, inflammation, oxidative stress, lipid metabolism and mitochondrial function, all of which have been implicated in ASD. In conclusion, our data are consistent with a molecular mechanism through which gut related environmental signals

  20. Nabokov's Details: Making Sense of Irrational Standards

    OpenAIRE

    Horgan, Pelagia

    2012-01-01

    Vladimir Nabokov's passion for detail is well-known, central to our very idea of the "Nabokovian." Yet Nabokov's most important claims for detail pose a challenge for the reader who would take them seriously. Startlingly extreme and deliberately counterintuitive -- Nabokov called them his "irrational standards" -- these claims push the very limits of reason and belief. Nabokov's critics have tended to treat his more extravagant claims for detail -- including his assertion that the "capacity t...

  1. Biokinetics and repeated exposure in in vitro assays : A detailed study into the behaviour of chlorpromazine and diazepam in different cell systems

    NARCIS (Netherlands)

    Broeders, J.J.W.

    2013-01-01

    The potential risk of compounds is commonly assessed with animal experimentation and extrapolation of these data to assess human health effects. The use of Integrated Testing o Strategies combines different methods, including in vitro tests and in silico methods, to perform risk assessment with less

  2. A study of aneuploidy and DNA fragmentation in spermatozoa of three men with sex chromosome mosaicism including a 45,X cell line.

    Science.gov (United States)

    Nguyen, Minh Huong; Morel, Frederic; Bujan, Louis; May-Panloup, Pascale; De Braekeleer, Marc; Perrin, Aurore

    2015-06-01

    Meiotic segregation of mosaic males with a 45,X cell line has been little examined. In this study, we evaluated the risk of aneuploid gametes using fluorescence in situ hybridization (FISH) and DNA fragmentation in ejaculated spermatozoa of three men with sex chromosome mosaicism including a 45,X cell line. Triple- and dual-color FISH were performed. Sperm DNA fragmentation was detected using the TUNEL assay. A significantly increased frequency of XY disomic spermatozoa was observed for patients (P)1 and P2. A significant increase in diploidy and autosomal aneuploidy was found in P2 and P3, respectively. The rate of DNA fragmentation was not different from that observed in a control group. Data from the literature are scarce (only 3 cases reported), making comparison of the present data difficult, especially as the frequencies of the cell lines comprising the mosaicism differed between patients. Furthermore, the proportion of the different cell lines can differ from one tissue to another in the same patient. Whether the relative levels of the several cell lines present in the mosaicism can influence the rate of aneuploid spermatozoa remains unknown.

  3. Detailed deletion mapping of loss of heterozygosity on 9p13-23 in laryngeal squamous cell carcinoma by microsatellite analysis

    Institute of Scientific and Technical Information of China (English)

    徐先发; 高燕宁; 程书鈞

    2004-01-01

    Background This study was designed to investigate the hot spots of microsatellite loss of heterozygosity (LOH) on 9p13-23 in laryngeal squamous cell carcinoma and to find out the correlation between the incidence of microsatellite LOH and the clinicopathological parameters.Methods Tumor tissues were obtained from paraffin embedded sections with microdissection. Genomic DNA was extracted from tumor tissues and peripheral blood lymphocytes with the phenol-chloroform. Polymerase chain reaction (PCR) amplification and denaturing gel electrophoresis were carried out in a set of 42 squamous cell carcinoma (SCC) of larynx and corresponding peripheral blood lymphocytes using 13 highly polymorphic microsatellite markers on 9p13-23. The correlation was analyzed between microsatellite LOH at the high frequency on 9p13-23 and clinicopathological parameters in the patients with squamous cell carcinoma of larynx.Results Of the 42 laryngeal cancers, 41 (97.6%) showed LOH in at least one of the microsatellite markers tested on 9p13-23. The most frequently deleted marker was D9S162 in 17 of the 19 (89.5%) informative samples. The marker D9S171, which is located on 9p21, had LOH detected in 12 of the 15 informative cases (80.0%). LOH at the D9S1748 marker (closest to the p16 gene locus) was detected in 18 of the 36 informative cases (50.0%). Allelic deletion mapping revealed two minimal regions of LOH encompassing markers D9S161-D9S171 on 9p21 and IFNA-D9S162 on 9p22-23. Multiple LOH (≥4) on 9p21-23 was found more frequently in the patients under 60 years, with supraglottic SCC or cervical lymph node metastasis than those over 60 years, with glottic SCC or without cervical lymph node metastasis (P<0.01 or 0.01, 0.05, respectively). On the contrary, there was no correlation between T stages or pathologic classification and the frequency of LOH on 9p21-23 in 42 SCC of Larynx.Conclusions These findings imply the presence of at least two putative tumor suppressor genes on 9p13-23 in

  4. Signal transduction profile of chemical sensitisers in dendritic cells: An endpoint to be included in a cell-based in vitro alternative approach to hazard identification?

    International Nuclear Information System (INIS)

    The development of non-animal testing methods for the assessment of skin sensitisation potential is an urgent challenge within the framework of existing and forthcoming legislation. Efforts have been made to replace current animal tests, but so far no alternative methods have been developed. It is widely recognised that alternatives to animal testing cannot be accomplished with a single approach, but rather will require the integration of results obtained from different in vitro and in silico assays. The argument subjacent to the development of in vitro dendritic cell (DC)-based assays is that sensitiser-induced changes in the DC phenotype can be differentiated from those induced by irritants. This assumption is derived from the unique capacity of DC to convert environmental signals encountered at the skin into a receptor expression pattern (MHC class II molecules, co-stimulatory molecules, chemokine receptors) and a soluble mediator release profile that will stimulate T lymphocytes. Since signal transduction cascades precede changes in surface marker expression and cytokine/chemokine secretion, these phenotypic modifications are a consequence of a signal transduction profile that is specifically triggered by sensitisers and not by irritants. A limited number of studies have addressed this subject and the present review attempts to summarise and highlight all of the signalling pathways modulated by skin sensitisers and irritants. Furthermore, we conclude this review by focusing on the most promising strategies suitable for inclusion into a cell-based in vitro alternative approach to hazard identification.

  5. Biokinetics and repeated exposure in in vitro assays : A detailed study into the behaviour of chlorpromazine and diazepam in different cell systems

    OpenAIRE

    Broeders, J.J.W.

    2013-01-01

    The potential risk of compounds is commonly assessed with animal experimentation and extrapolation of these data to assess human health effects. The use of Integrated Testing o Strategies combines different methods, including in vitro tests and in silico methods, to perform risk assessment with less costs and less animal experiments. Although alternatives to animal tests have been developed and validated, research into alternatives for certain toxicological endpoints is yet limited. One of th...

  6. The conjectured S-type retrograde planet in nu Octantis: more evidence including four years of iodine-cell radial velocities

    CERN Document Server

    Ramm, D J; Endl, M; Hearnshaw, J B; Wittenmyer, R A; Gunn, F; Bergmann, C; Kilmartin, P; Brogt, E

    2016-01-01

    We report 1212 radial-velocity (RV) measurements obtained in the years 2009-2013 using an iodine cell for the spectroscopic binary nu Octantis (K1III/IV). This system (a_bin~2.6 au, P~1050 days) is conjectured to have a Jovian planet with a semi-major axis half that of the binary host. The extreme geometry only permits long-term stability if the planet is in a retrograde orbit. Whilst the reality of the planet (P~415 days) remains uncertain, other scenarios (stellar variability or apsidal motion caused by a yet unobserved third star) continue to appear substantially less credible based on CCF bisectors, line-depth ratios and many other independent details. If this evidence is validated but the planet is disproved, the claims of other planets using RVs will be seriously challenged. We also describe a significant revision to the previously published RVs and the full set of 1437 RVs now encompasses nearly 13 years. The sensitive orbital dynamics allow us to constrain the three-dimensional architecture with a bro...

  7. Tumor-specific hypermethylation of epigenetic biomarkers, including SFRP1, predicts for poorer survival in patients from the TCGA Kidney Renal Clear Cell Carcinoma (KIRC project.

    Directory of Open Access Journals (Sweden)

    Christopher J Ricketts

    Full Text Available The recent publication of the TCGA Kidney Renal Clear Cell Carcinoma (KIRC project has provided an immense wealth and breadth of data providing an invaluable tool for confirmation and expansion upon previous observations in a large data set containing multiple data types including DNA methylation, somatic mutation, and clinical information. In clear cell renal cell carcinoma (CCRCC many genes have been demonstrated to be epigenetically inactivated by promoter hypermethylated and in a small number of cases to be associated with clinical outcome. This study created two cohorts based on the Illumina BeadChip array used to confirm the frequency of tumor-specific hypermethylation of these published hypermethylated genes, assess the impact of somatic mutation or chromosomal loss and provide the most comprehensive assessment to date of the association of this hypermethylation with patient survival. Hypermethylation of the Fibrillin 2 (FBN2 gene was the most consistent epigenetic biomarker for CCRCC across both cohorts in 40.2% or 52.5% of tumors respectively. Hypermethylation of the secreted frizzled-related protein 1 (SFRP1 gene and the basonuclin 1 (BNC1 gene were both statistically associated with poorer survival in both cohorts (SFRP1 - p = <0.0001 or 0.0010 and BNC1 - p = <0.0001 or 0.0380 and represented better independent markers of survival than tumor stage, grade or dimension in one cohort and tumor stage or dimension in the other cohort. Loss of the SFRP1 protein can potentially activate the WNT pathway and this analysis highlighted hypermethylation of several other WNT pathway regulating genes and demonstrated a poorer survival outcome for patients with somatic mutation of these genes. The success of demethylating drugs in hematological malignances and the current trials in solid tumors suggest that the identification of clinically relevant hypermethylated genes combined with therapeutic advances may improve the effectiveness and

  8. Nisin production in a chitin-including continuous fermentation system with Lactococcus lactis displaying a cell wall chitin-binding domain.

    Science.gov (United States)

    Şimşek, Ömer

    2014-03-01

    The limiting factors in the continuous production of nisin are high amount of biomass loss and low dilution rate application. In this study, a chitin-including continuous nisin fermentation system (CICON-FER) was constructed for high volumetric nisin production using nisin producer L. lactis displaying cell wall chitin-binding domain (ChBD) together with chitin in the reactor. In this respect, the highest binding conditions of relevant L. lactis cells to chitin were determined. Then the chitin flakes carrying nisin-producing L. lactis cells were used within the CICON-FER system at different dilution rates (0.1-0.9 h⁻¹) and initial glucose concentrations (20-60 g l⁻¹). The results revealed that the pH 7 conditions and the use of 100 mM sodium phosphate buffer with 0.1 % Tween 20 and Triton X-100 significantly increased the binding capacity of ChBD displaying L. lactis cells to chitin. The constructed CICON-FER system maintained the presence of the ChBD surface displaying L. lactis cells in the reactor system until 0.9 h⁻¹ dilution rate that resulted in a considerably high level of volumetric nisin production and productivity (10,500 IU ml⁻¹ and 9,450 IU ml⁻¹ h⁻¹, respectively) with the combination of a 0.9-h⁻¹ dilution rate and a 40-g l⁻¹ initial glucose concentration. In conclusion, an innovative nisin fermentation system that yielded the highest nisin production thus far and that was feasible for industrial application was created.

  9. A systems-level approach to parental genomic imprinting: the imprinted gene network includes extracellular matrix genes and regulates cell cycle exit and differentiation.

    Science.gov (United States)

    Al Adhami, Hala; Evano, Brendan; Le Digarcher, Anne; Gueydan, Charlotte; Dubois, Emeric; Parrinello, Hugues; Dantec, Christelle; Bouschet, Tristan; Varrault, Annie; Journot, Laurent

    2015-03-01

    Genomic imprinting is an epigenetic mechanism that restrains the expression of ∼ 100 eutherian genes in a parent-of-origin-specific manner. The reason for this selective targeting of genes with seemingly disparate molecular functions is unclear. In the present work, we show that imprinted genes are coexpressed in a network that is regulated at the transition from proliferation to quiescence and differentiation during fibroblast cell cycle withdrawal, adipogenesis in vitro, and muscle regeneration in vivo. Imprinted gene regulation is not linked to alteration of DNA methylation or to perturbation of monoallelic, parent-of-origin-dependent expression. Overexpression and knockdown of imprinted gene expression alters the sensitivity of preadipocytes to contact inhibition and adipogenic differentiation. In silico and in cellulo experiments showed that the imprinted gene network includes biallelically expressed, nonimprinted genes. These control the extracellular matrix composition, cell adhesion, cell junction, and extracellular matrix-activated and growth factor-activated signaling. These observations show that imprinted genes share a common biological process that may account for their seemingly diverse roles in embryonic development, obesity, diabetes, muscle physiology, and neoplasm.

  10. The odyssey of Hsp60 from tumor cells to other destinations includes plasma membrane-associated stages and Golgi and exosomal protein-trafficking modalities.

    Directory of Open Access Journals (Sweden)

    Claudia Campanella

    Full Text Available BACKGROUND: In a previous work we showed for the first time that human tumor cells secrete Hsp60 via exosomes, which are considered immunologically active microvesicles involved in tumor progression. This finding raised questions concerning the route followed by Hsp60 to reach the exosomes, its location in them, and whether Hsp60 can be secreted also via other mechanisms, e.g., by the Golgi. We addressed these issues in the work presented here. PRINCIPAL FINDINGS: We found that Hsp60 localizes in the tumor cell plasma membrane, is associated with lipid rafts, and ends up in the exosomal membrane. We also found evidence that Hsp60 localizes in the Golgi apparatus and its secretion is prevented by an inhibitor of this organelle. CONCLUSIONS/SIGNIFICANCE: We propose a multistage process for the translocation of Hsp60 from the inside to the outside of the cell that includes a combination of protein traffic pathways and, ultimately, presence of the chaperonin in the circulating blood. The new information presented should help in designing future strategies for research and for developing diagnostic-monitoring means useful in clinical oncology.

  11. On Detailing in Contemporary Architecture

    DEFF Research Database (Denmark)

    Kristensen, Claus; Kirkegaard, Poul Henning

    2010-01-01

    Details in architecture have a significant influence on how architecture is experienced. One can touch the materials and analyse the detailing - thus details give valuable information about the architectural scheme as a whole. The absence of perceptual stimulation like details and materiality...... / tactility can blur the meaning of the architecture and turn it into an empty statement. The present paper will outline detailing in contemporary architecture and discuss the issue with respect to architectural quality. Architectural cases considered as sublime piece of architecture will be presented...

  12. Antiproliferative Effects of New Dimeric Ellagitannin from Cornus alba in Prostate Cancer Cells Including Apoptosis-Related S-Phase Arrest.

    Science.gov (United States)

    Park, Kwan Hee; Yin, Jun; Yoon, Ki Hoon; Hwang, Yoon Jeong; Lee, Min Won

    2016-01-01

    Activity-guided isolation of 80% acetone extract of Cornus alba, which is traditionally used as an anti-inflammatory, hemostatic and diuretic in Korea, yielded one novel compound, tentatively designated cornusiin H (13), together with 12 known compounds. The known compounds included four flavonoids (catechin (1), quercetin-3-O-β-D-glucuronide (2), quercetin-3-O-β-D-glucopyranoside (3), kaempferol-3-O-β-D-glucopyranoside (4)) and eight hydrolysable tannins (gallic acid (5), 2,6-di-O-galloyl-hamamelofuranoside (6), 2-galloyl-4-caffeoyl-L-threonic acid (7) 2,3-di-O-galloyl-4-caffeoyl-L-threonic acid (8), 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranoside (9), cornusiin B (10), cornusiin A (11) and camptothin B (12)). All compounds exhibited potent 1,1-diphenyl-2-picrylhydrazyl (DPPH)-free radical scavenging activity. Especially, the radical scavenging activities of 6 and 9-13 were higher than that of vitamin C. Compounds 9, 11, 12 and 13 inhibited the production of nitric oxide (NO) in lipopolysaccharide-stimulated RAW264.7 cells to the same degree as N(G)-Monomethyl-L-arginine (L-NMMA). When the antiproliferative effects of the isolated compounds were assessed in prostate cancer cells, the dimeric ellagitannins (11-13) selectively inhibited LNCaP hormone-dependent prostate cancer cells. Flow cytometry analysis indicated that the dimeric ellagitannins induced apoptosis and S-phase arrest. These results suggest that dimeric ellagitannins from Cornus alba can be developed as functional materials or herbal medicines for prostate tumors such as benign prostate hyperplasia and early-stage prostate cancer. PMID:26805810

  13. Antiproliferative Effects of New Dimeric Ellagitannin from Cornus alba in Prostate Cancer Cells Including Apoptosis-Related S-Phase Arrest

    Directory of Open Access Journals (Sweden)

    Kwan Hee Park

    2016-01-01

    Full Text Available Activity-guided isolation of 80% acetone extract of Cornus alba, which is traditionally used as an anti-inflammatory, hemostatic and diuretic in Korea, yielded one novel compound, tentatively designated cornusiin H (13, together with 12 known compounds. The known compounds included four flavonoids (catechin (1, quercetin-3-O-β-d-glucuronide (2, quercetin-3-O-β-d-glucopyranoside (3, kaempferol-3-O-β-d-glucopyranoside (4 and eight hydrolysable tannins (gallic acid (5, 2,6-di-O-galloyl-hamamelofuranoside (6, 2-galloyl-4-caffeoyl-l-threonic acid (7 2,3-di-O-galloyl-4-caffeoyl-l-threonic acid (8, 1,2,3,4,6-penta-O-galloyl-β-d-glucopyranoside (9, cornusiin B (10, cornusiin A (11 and camptothin B (12. All compounds exhibited potent 1,1-diphenyl-2-picrylhydrazyl (DPPH-free radical scavenging activity. Especially, the radical scavenging activities of 6 and 9–13 were higher than that of vitamin C. Compounds 9, 11, 12 and 13 inhibited the production of nitric oxide (NO in lipopolysaccharide-stimulated RAW264.7 cells to the same degree as NG-Monomethyl-l-arginine (l-NMMA. When the antiproliferative effects of the isolated compounds were assessed in prostate cancer cells, the dimeric ellagitannins (11–13 selectively inhibited LNCaP hormone-dependent prostate cancer cells. Flow cytometry analysis indicated that the dimeric ellagitannins induced apoptosis and S-phase arrest. These results suggest that dimeric ellagitannins from Cornus alba can be developed as functional materials or herbal medicines for prostate tumors such as benign prostate hyperplasia and early-stage prostate cancer.

  14. A novel BH3 mimetic efficiently induces apoptosis in melanoma cells through direct binding to anti-apoptotic Bcl-2 family proteins, including phosphorylated Mcl-1.

    Science.gov (United States)

    Liu, Yubo; Xie, Mingzhou; Song, Ting; Sheng, Hongkun; Yu, Xiaoyan; Zhang, Zhichao

    2015-03-01

    The Bcl-2 family modulates sensitivity to chemotherapy in many cancers, including melanoma, in which the RAS/BRAF/MEK/ERK pathway is constitutively activated. Mcl-1, a major anti-apoptotic protein in the Bcl-2 family, is extensively expressed in melanoma and contributes to melanoma's well-documented chemoresistance. Here, we provide the first evidence that Mcl-1 phosphorylation at T163 by ERK1/2 and JNK is associated with the resistance of melanoma cell lines to the existing BH3 mimetics gossypol, S1 and ABT-737, and a novel anti-apoptotic mechanism of phosphorylated Mcl-1 (pMcl-1) is revealed. pMcl-1 antagonized the known BH3 mimetics by sequestering pro-apoptotic proteins that were released from Bcl-2/Mcl-1. Furthermore, an anthraquinone BH3 mimetic, compound 6, was identified to be the first small molecule to that induces endogenous apoptosis in melanoma cells by directly binding Bcl-2, Mcl-1, and pMcl-1 and disrupting the heterodimers of these proteins. Although compound 6 induced upregulation of the pro-apoptotic protein Noxa, its apoptotic induction was independent of Noxa. These data reveal the promising therapeutic potential of targeting pMcl-1 to treat melanoma. Compound 6 is therefore a potent drug that targets pMcl-1 in melanoma.

  15. Verrucous carcinomas of the head and neck, including those with associated squamous cell carcinoma, lack transcriptionally active high-risk human papillomavirus.

    Science.gov (United States)

    Patel, Kalyani R; Chernock, Rebecca D; Zhang, Tian R; Wang, Xiaowei; El-Mofty, Samir K; Lewis, James S

    2013-11-01

    Most oropharyngeal squamous cell carcinomas (SCC) and histologic variants harbor transcriptionally active human papillomavirus (HPV). While HPV DNA can be found in many non-oropharyngeal head and neck carcinomas, transcriptionally active HPV is rare. Verrucous carcinoma is a variant with bland cytology, warty appearance, locally destructive growth, and lack of metastasis when lacking a frankly invasive carcinoma component. Studies have shown variable rates of HPV DNA and p16 protein expression in such tumors but still have not clearly addressed if the virus has biological activity or clinical relevance in the positive cases. Department files were searched for verrucous neoplasms, including pure verrucous carcinoma, verrucous carcinoma with dysplasia or minimal invasion, and SCC arising in verrucous carcinoma (ie, having a major component of frankly invasive carcinoma). p16 immunohistochemistry, HPV DNA polymerase chain reaction (PCR) and E6/E7 mRNA reverse transcription PCR for high-risk HPV types were performed. Of the 49 cases, 6 (12.2%) showed strong (>50%) staining for p16. HPV DNA was detected in 7/49 (14.3%) cases, but only one case was positive for both p16, and HPV DNA. A total of 36 cases yielded sufficient RNA for RT-PCR (18 verrucous carcinomas, 13 atypical verrucous carcinomas, and 5 SCC arising in verrucous carcinoma). All 36 were negative, including the four p16-positive and three HPV DNA-positive tumors tested. Although a minority of verrucous carcinoma lesions are p16 and HPV DNA positive, transcriptionally active high-risk HPV is uniformly absent. These findings argue that verrucous carcinoma and its related squamous cell carcinomas are not HPV-driven tumors.

  16. The fine details of evolution.

    Science.gov (United States)

    Laskowski, Roman A; Thornton, Janet M; Sternberg, Michael J E

    2009-08-01

    Charles Darwin's theory of evolution was based on studies of biology at the species level. In the time since his death, studies at the molecular level have confirmed his ideas about the kinship of all life on Earth and have provided a wealth of detail about the evolutionary relationships between different species and a deeper understanding of the finer workings of natural selection. We now have a wealth of data, including the genome sequences of a wide range of organisms, an even larger number of protein sequences, a significant knowledge of the three-dimensional structures of proteins, DNA and other biological molecules, and a huge body of information about the operation of these molecules as systems in the molecular machinery of all living things. This issue of Biochemical Society Transactions contains papers from oral presentations given at a Biochemical Society Focused Meeting to commemorate the 200th Anniversary of Charles Darwin's birth, held on 26-27 January 2009 at the Wellcome Trust Conference Centre, Cambridge. The talks reported on some of the insights into evolution which have been obtained from the study of protein sequences, structures and systems. PMID:19614583

  17. Computed tomography:the details.

    Energy Technology Data Exchange (ETDEWEB)

    Doerry, Armin Walter

    2007-07-01

    Computed Tomography (CT) is a well established technique, particularly in medical imaging, but also applied in Synthetic Aperture Radar (SAR) imaging. Basic CT imaging via back-projection is treated in many texts, but often with insufficient detail to appreciate subtleties such as the role of non-uniform sampling densities. Herein are given some details often neglected in many texts.

  18. Optimizing Staining Protocols for Laser Microdissection of Specific Cell Types from the Testis Including Carcinoma In Situ

    OpenAIRE

    Si Brask Sonne; Dalgaard, Marlene D; John Erik Nielsen; Hoei-Hansen, Christina E.; Ewa Rajpert-De Meyts; Lise Mette Gjerdrum; Henrik Leffers

    2009-01-01

    Udgivelsesdato: May 2009 Microarray and RT-PCR based methods are important tools for analysis of gene expression; however, in tissues containing many different cells types, such as the testis, characterization of gene expression in specific cell types can be severely hampered by noise from other cells. The laser microdissection technology allows for enrichment of specific cell types. However, when the cells are not morphologically distinguishable, it is necessary to use a specific staining...

  19. Detailed Electrochemical Characterisation of Large SOFC Stacks

    DEFF Research Database (Denmark)

    Mosbæk, Rasmus Rode; Hjelm, Johan; Barfod, R.;

    2012-01-01

    application of advanced methods for detailed electrochemical characterisation during operation. An operating stack is subject to steep compositional gradients in the gaseous reactant streams, and significant temperature gradients across each cell and across the stack, which makes it a complex system...... Fuel Cell A/S was characterised in detail using electrochemical impedance spectroscopy. An investigation of the optimal geometrical placement of the current probes and voltage probes was carried out in order to minimise measurement errors caused by stray impedances. Unwanted stray impedances...... are particularly problematic at high frequencies. Stray impedances may be caused by mutual inductance and stray capacitance in the geometrical set-up and do not describe the fuel cell. Three different stack geometries were investigated by electrochemical impedance spectroscopy. Impedance measurements were carried...

  20. Optoelectronic pH Meter: Further Details

    Science.gov (United States)

    Jeevarajan, Antony S.; Anderson, Mejody M.; Macatangay, Ariel V.

    2009-01-01

    A collection of documents provides further detailed information about an optoelectronic instrument that measures the pH of an aqueous cell-culture medium to within 0.1 unit in the range from 6.5 to 7.5. The instrument at an earlier stage of development was reported in Optoelectronic Instrument Monitors pH in a Culture Medium (MSC-23107), NASA Tech Briefs, Vol. 28, No. 9 (September 2004), page 4a. To recapitulate: The instrument includes a quartz cuvette through which the medium flows as it is circulated through a bioreactor. The medium contains some phenol red, which is an organic pH-indicator dye. The cuvette sits between a light source and a photodetector. [The light source in the earlier version comprised red (625 nm) and green (558 nm) light-emitting diodes (LEDs); the light source in the present version comprises a single green- (560 nm)-or-red (623 nm) LED.] The red and green are repeatedly flashed in alternation. The responses of the photodiode to the green and red are processed electronically to obtain the ratio between the amounts of green and red light transmitted through the medium. The optical absorbance of the phenol red in the green light varies as a known function of pH. Hence, the pH of the medium can be calculated from the aforesaid ratio.

  1. Predictive value of diffusion-weighted imaging without and with including contrast-enhanced magnetic resonance imaging in image analysis of head and neck squamous cell carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Noij, Daniel P., E-mail: d.noij@vumc.nl [Department of Radiology and Nuclear Medicine, VU University Medical Center, De Boelelaan 1117, Amsterdam, Noord-Holland (Netherlands); Pouwels, Petra J.W., E-mail: pjw.pouwels@vumc.nl [Department of Physics and Medical Technology, VU University Medical Center, De Boelelaan 1117, Amsterdam, Noord-Holland (Netherlands); Ljumanovic, Redina, E-mail: rljumanovic@adventh.org [Department of Radiology and Nuclear Medicine, VU University Medical Center, De Boelelaan 1117, Amsterdam, Noord-Holland (Netherlands); Knol, Dirk L., E-mail: dirklknol@gmail.com [Department of Epidemiology and Biostatistics, VU University Medical Center, De Boelelaan 1117, Amsterdam, Noord-Holland (Netherlands); Doornaert, Patricia, E-mail: p.doornaert@vumc.nl [Department of Radiation Oncology, VU University Medical Center, De Boelelaan 1117, Amsterdam, Noord-Holland (Netherlands); Bree, Remco de, E-mail: r.debree@vumc.nl [Department of Otolaryngology – Head and Neck Surgery, VU University Medical Center, De Boelelaan 1117, Amsterdam, Noord-Holland (Netherlands); Castelijns, Jonas A., E-mail: j.castelijns@vumc.nl [Department of Radiology and Nuclear Medicine, VU University Medical Center, De Boelelaan 1117, Amsterdam, Noord-Holland (Netherlands); Graaf, Pim de, E-mail: p.degraaf@vumc.nl [Department of Radiology and Nuclear Medicine, VU University Medical Center, De Boelelaan 1117, Amsterdam, Noord-Holland (Netherlands)

    2015-01-15

    Highlights: • Primary tumor volume and lymph node ADC1000 are predictors of survival. • CE-T1WI does not improve the prognostic capacity of DWI. • Using CE-T1WI for ROI placement results in lower interobserver agreement. - Abstract: Objectives: To assess disease-free survival (DFS) in head and neck squamous cell carcinoma (HNSCC) treated with (chemo)radiotherapy ([C]RT). Methods: Pretreatment MR-images of 78 patients were retrospectively studied. Apparent diffusion coefficients (ADC) were calculated with two sets of two b-values: 0–750 s/mm{sup 2} (ADC{sub 750}) and 0–1000 s/mm{sup 2} (ADC{sub 1000}). One observer assessed tumor volume on T1-WI. Two independent observers assessed ADC-values of primary tumor and largest lymph node in two sessions (i.e. without and with including CE-T1WI in image analysis). Interobserver and intersession agreement were assessed with intraclass correlation coefficients (ICC) separately for ADC{sub 750} and ADC{sub 1000}. Lesion volumes and ADC-values were related to DFS using Cox regression analysis. Results: Median follow-up was 18 months. Interobserver ICC was better without than with CE-T1WI (primary tumor: 0.92 and 0.75–0.83, respectively; lymph node: 0.81–0.83 and 0.61–0.64, respectively). Intersession ICC ranged from 0.84 to 0.89. With CE-T1WI, mean ADC-values of primary tumor and lymph node were higher at both b-values than without CE-T1WI (P < 0.001). Tumor volume (sensitivity: 73%; specificity: 57%) and lymph node ADC{sub 1000} (sensitivity: 71–79%; specificity: 77–79%) were independent significant predictors of DFS without and with including CE-T1WI (P < 0.05). Conclusions: Pretreatment primary tumor volume and lymph node ADC{sub 1000} were significant independent predictors of DFS in HNSCC treated with (C)RT. DFS could be predicted from ADC-values acquired without and with including CE-T1WI in image analysis. The inclusion of CE-T1WI did not result in significant improvements in the predictive value of

  2. Phonetic Detail in American English

    Institute of Scientific and Technical Information of China (English)

    Ray Freeze

    1987-01-01

    @@ In the course of teaching general phonetics and phonological analysis in the psat few years,l have found some phonetic detail which some native speakers as well as non-native speakers were unaware of. This subtle detail will be the focus of this presentation. Som e of this detail many of you will already be aware of because of your experience in learning, teaching, and thinking about English. If anything is new to you, I hope you might enjoy hearing about it even if it turns out not to be useful in your work.

  3. The influence of bone surface availability in bone remodelling - A mathematical model including coupled geometrical and biomechanical regulations of bone cells

    OpenAIRE

    Pivonka, Peter; Buenzli, Pascal R.; Scheiner, Stefan; Hellmich, Christian; Dunstan, Colin R.

    2012-01-01

    Bone is a biomaterial undergoing continuous renewal. The renewal process is known as bone remodelling and is operated by bone-resorbing cells (osteoclasts) and bone-forming cells (osteoblasts). Both biochemical and biomechanical regulatory mechanisms have been identified in the interaction between osteoclasts and osteoblasts. Here we focus on an additional and poorly understood potential regulatory mechanism of bone cells, that involves the morphology of the microstructure of bone. Bone cells...

  4. Diethylnitrosamine-induced expression of germline-specific genes and pluripotency factors, including vasa and oct4, in medaka somatic cells.

    Science.gov (United States)

    Shen, Jialing; Yokota, Shinpei; Yokoi, Hayato; Suzuki, Tohru

    2016-09-16

    Various methods have been developed to reprogram mammalian somatic cells into pluripotent cells as well as to directly reprogram somatic cells into other cell lineages. We are interested in applying these methods to fish, and here, we examined whether mRNA expression of germline-specific genes (vasa, nanos2, -3) and pluripotency factors (oct4, sox2, c-myc, nanog) is inducible in somatic cells of Japanese medaka (Oryzias latipes). We found that the expression of vasa is induced in the gut and regenerating fin by exposure to a carcinogen, diethylnitrosamine (DEN). Induction of vasa in the gut started on the 5th day of treatment with >50 ppm DEN. In addition, nanos2, -3, oct4, sox2, klf4, c-myc, and nanog were also expressed simultaneously in some vasa-positive gut and regenerating fin samples. Vasa-positive cells were detected by immunohistochemistry (IHC) in the muscle surrounding the gut and in the wound epidermis, blastema, and fibroblast-like cells in regenerating fin. In vasa:GFP transgenic medaka, green fluorescent protein (GFP) fluorescence appeared in the wound epidermis and fibroblast-like cells in the regenerating fin following DEN exposure, in agreement with the IHC data. Our data show that mRNA expression of genes relevant to germ cell specification and pluripotency can be induced in fish somatic cells by exposure to DEN, suggesting the possibility of efficient and rapid cell reprogramming of fish somatic cells. PMID:27514449

  5. No evidence for a different magnitude of the time factor for continuously fractionated irradiation and protocols including gaps in two human squamous cell carcinoma in nude mice

    International Nuclear Information System (INIS)

    Background and purpose: To study whether the magnitude of the time factor is different for continuously fractionated irradiation and for fractionation protocols including gaps. Materials and methods: Two human head and neck squamous cell carcinomas (SCCs), FaDu and GL, were transplanted subcutaneously into the right hindleg of NMRI (nu/nu) mice and irradiated with 30 fractions under ambient conditions within 2, 6 and 10 weeks. Irradiations within 6 and 10 weeks were given either as a continuous course or with a mid-course gap of 3 weeks. The end-point of the experiments was local tumor control at day 120 (FaDu) or day 180 (GL) after the end of treatment. Results: In FaDu tumors, two experimental cohorts (A, B) yielded significantly different results and were analyzed separately. In cohort A, the tumor control dose 50% (TCD50) increased from 37 to 89 Gy when the treatment time of continuous fractionated irradiation was extended from 2 to 10 weeks. The recovered dose/day (Dr) was 0.98 Gy (95% confidence interval, 0.72; 1.27). In cohort B, the TCD50 increased from 35 to 63 Gy, and the Dr was 0.51 Gy (0.24; 0.75). In GL tumors, the TCD50 for continuously fractionated irradiation increased from 41 to 48 Gy. This increase was not significant, and the Dr was 0.15 Gy (0; 0.30). None of the TCD50 and Dr values obtained in both tumor models for continuous irradiation vs. irradiation with a gap were significantly different. Conclusions: Prolongation of the overall treatment time of fractionated irradiation resulted in a pronounced decrease of local control in human FaDu SCC and little decrease of local control in human GL SCC. No evidence was found that the magnitude of the time factor in these tumors is different for continuous fractionation or fractionation protocols including gaps

  6. 细致平衡理论计算CdS/CdTe异质结太阳电池的极限转换效率%DETAIL BALANCE LIMIT OF EFFICIENCY OF CdS/CdTe HETEROJUNCTION SOLAR CELLS

    Institute of Scientific and Technical Information of China (English)

    熊超; 陈磊; 袁洪春; 姚若河

    2013-01-01

    Based on detailed balance theory and considered the barriers at interface of heterojunction,which result from the band offset and block photon-generated carriers transport,a photoelectric conversion model for heterojunction solar cells was established.The problem of the limit efficiency of heterojunction solar cells was solved using the detailed balance theory.A method to realize the Ⅰ-Ⅴ characteristics of the CdS/CdTe heterojunction solar cells under the illumination and the limit conversion efficiency of CdS/CdTe solar cells was proposed in this article.Under the illumination,the Ⅰ-Ⅴ characteristics and maximum conversion efficiency are closely related to the built-in barrier of the CdS/CdTe solar cells.It is shown that a theoretical efficiency limit of 29% can be achieved for CdS/CdTe heterojunction solar cells under AM1.5 illumination.Considering the typical parameters of CdS and CdTe,the maximum conversion efficiency of 25% can be achieved.Analysis of the reality of the efficiency is not high and the feasibility of improving conversion efficiency of the CdS/CdTe heterojunction solar cells.%在细致平衡理论基础上,针对异质结电池在结处由于能带不连续而形成载流子运动势垒可能对光生载流子的运输存在阻碍作用,建立异质结太阳电池的光电转换模型,解决了细致平衡理论无法计算异质结太阳电池极限效率的问题,推导出CdS/CdTe异质结太阳电池光照下的Ⅰ-Ⅴ特性表达式以及最大光电转换效率的求法.指出电池的Ⅰ-Ⅴ特性以及最大转换效率与电池的内建势垒密切相关,并求出在理想情况AM1.5的光照下CdS/CdTe异质结太阳电池最大转换效率可达29%;考虑到CdS和CdTe的典型参数后,计算出其最大转换效率可达25%.分析了现实中电池效率不高的原因,并指出实现电池转换效率提高的可行性.

  7. Studies on cell migration, adenylate cyclase and membrane-coating granules in the buccal epithelium of the zinc-deficient rabbit, including the influence of isoproterenol.

    Science.gov (United States)

    Chen, S Y

    1988-01-01

    Cell migration was slightly increased; cytochemical reaction deposits of adenylate cyclase and the area density of membrane-coating granules (MCG) were significantly increased. Upon isoproterenol stimulation, the MCG area density was significantly increased, whereas the cell migration rate was unchanged. Thus in zinc deficiency, there may be a simultaneous increase in the production and secretion of MCGs, in adenylate cyclase activity, and in cell migration. The non-significantly increased cell migration rate may not keep pace with the significantly increased cell-production rate, resulting in thickening of the epithelium.

  8. Whole-body-MR imaging including DWIBS in the work-up of patients with head and neck squamous cell carcinoma: A feasibility study

    Energy Technology Data Exchange (ETDEWEB)

    Noij, Daniel P., E-mail: d.noij@vumc.nl [Department of Radiology and Nuclear Medicine, VU University Medical Center, De Boelelaan 1117, PO Box 7057, 1007 MB Amsterdam (Netherlands); Boerhout, Els J., E-mail: e.boerhout@vumc.nl [Department of Radiology and Nuclear Medicine, VU University Medical Center, De Boelelaan 1117, PO Box 7057, 1007 MB Amsterdam (Netherlands); Pieters-van den Bos, Indra C., E-mail: i.pieters@vumc.nl [Department of Radiology and Nuclear Medicine, VU University Medical Center, De Boelelaan 1117, PO Box 7057, 1007 MB Amsterdam (Netherlands); Comans, Emile F., E-mail: efi.comans@vumc.nl [Department of Radiology and Nuclear Medicine, VU University Medical Center, De Boelelaan 1117, PO Box 7057, 1007 MB Amsterdam (Netherlands); Oprea-Lager, Daniela, E-mail: d.oprea-lager@vumc.nl [Department of Radiology and Nuclear Medicine, VU University Medical Center, De Boelelaan 1117, PO Box 7057, 1007 MB Amsterdam (Netherlands); Reinhard, Rinze, E-mail: r.reinhard@vumc.nl [Department of Radiology and Nuclear Medicine, VU University Medical Center, De Boelelaan 1117, PO Box 7057, 1007 MB Amsterdam (Netherlands); Hoekstra, Otto S., E-mail: os.hoekstra@vumc.nl [Department of Radiology and Nuclear Medicine, VU University Medical Center, De Boelelaan 1117, PO Box 7057, 1007 MB Amsterdam (Netherlands); Bree, Remco de, E-mail: r.debree@vumc.nl [Department Otolaryngology/Head and Neck Surgery, VU University Medical Center, De Boelelaan 1117, PO Box 7057, 1007 MB Amsterdam (Netherlands); Graaf, Pim de, E-mail: p.degraaf@vumc.nl [Department of Radiology and Nuclear Medicine, VU University Medical Center, De Boelelaan 1117, PO Box 7057, 1007 MB Amsterdam (Netherlands); Castelijns, Jonas A., E-mail: j.castelijns@vumc.nl [Department of Radiology and Nuclear Medicine, VU University Medical Center, De Boelelaan 1117, PO Box 7057, 1007 MB Amsterdam (Netherlands)

    2014-07-15

    Objectives: To assess the feasibility of whole-body magnetic resonance imaging (WB-MRI) including diffusion-weighted whole-body imaging with background-body-signal-suppression (DWIBS) for the evaluation of distant malignancies in head and neck squamous cell carcinoma (HNSCC); and to compare WB-MRI findings with {sup 18}F-fluorodeoxyglucose positron emission tomography/computed tomography ({sup 18}F-FDG-PET/CT) and chest-CT. Methods: Thirty-three patients with high risk for metastatic spread (26 males; range 48–79 years, mean age 63 ± 7.9 years (mean ± standard deviation) years) were prospectively included with a follow-up of six months. WB-MRI protocol included short-TI inversion recovery and T1-weighted sequences in the coronal plane and half-fourier acquisition single-shot turbo spin-echo T2 and contrast-enhanced-T1-weighted sequences in the axial plane. Axial DWIBS was reformatted in the coronal plane. Interobserver variability was assessed using weighted kappa and the proportion specific agreement (PA). Results: Two second primary tumors and one metastasis were detected on WB-MRI. WB-MRI yielded seven clinically indeterminate lesions which did not progress at follow-up. The metastasis and one second primary tumor were found when combining {sup 18}F-FDG-PET/CT and chest-CT findings. Interobserver variability for WB-MRI was κ = 0.91 with PA ranging from 0.82 to 1.00. For {sup 18}F-FDG-PET/CT κ could not be calculated due to a constant variable in the table and PA ranged from 0.40 to 0.99. Conclusions: Our WB-MRI protocol with DWIBS is feasible in the work-up of HNSCC patients for detection and characterization of distant pathology. WB-MRI can be complementary to {sup 18}F-FDG-PET/CT, especially in the detection of non {sup 18}F-FDG avid second primary tumors.

  9. The alkaline single cell electrophoresis assay with eight mouse organs: results with 22 mono-functional alkylating agents (including 9 dialkyl N-nitrosoamines) and 10 DNA crosslinkers.

    Science.gov (United States)

    Tsuda, S; Matsusaka, N; Madarame, H; Miyamae, Y; Ishida, K; Satoh, M; Sekihashi, K; Sasaki, Y F

    2000-04-13

    The genotoxicity of 22 mono-functional alkylating agents (including 9 dialkyl N-nitrosoamines) and 10 DNA crosslinkers selected from IARC (International Agency for Research on Cancer) groups 1, 2A, and 2B was evaluated in eight mouse organs with the alkaline single cell gel electrophoresis (SCGE) (comet) assay. Groups of four mice were treated once intraperitoneally at the dose at which micronucleus tests had been conducted, and the stomach, colon, liver, kidney, bladder, lung, brain, and bone marrow were sampled 3, 8, and/or 24 h later. All chemicals were positive in the SCGE assay in at least one organ. Of the 22 mono-functional alkylating agents, over 50% were positive in all organs except the brain and bone marrow. The two subsets of mono-functional alkylating agents differed in their bone marrow genotoxicity: only 1 of the 9 dialkyl N-nitrosoamines was positive in bone marrow as opposed to 8 of the 13 other alkylating agents, reflecting the fact that dialkyl N-nitrosoamines are poor micronucleus inducers in hematopoietic cells. The two groups of mono-functional alkylating agents also differ in hepatic carcinogenicity in spite of the fact that they are similar in hepatic genotoxicity. While dialkyl N-nitrosoamines produce tumors primarily in mouse liver, only one (styrene-7,8-oxide) out of 10 of the other type of mono-functional alkylating agents is a mouse hepatic carcinogen. Taking into consideration our previous results showing high concordance between hepatic genotoxicity and carcinogenicity for aromatic amines and azo compounds, a possible explanation for the discrepancy might be that chemicals that require metabolic activation show high concordance between genotoxicity and carcinogenicity in the liver. A high percent of the 10 DNA crosslinkers were positive in the SCGE assay in the gastrointestinal mucosa, but less than 50% were positive in the liver and lung. In this study, we allowed 10 min alkali-unwinding to obtain low and stable control values

  10. The role of versican G3 domain in regulating breast cancer cell motility including effects on osteoblast cell growth and differentiation in vitro – evaluation towards understanding breast cancer cell bone metastasis

    Directory of Open Access Journals (Sweden)

    Du William

    2012-08-01

    Full Text Available Abstract Background Versican is detected in the interstitial tissues at the invasive margins of breast carcinoma, is predictive of relapse, and negatively impacts overall survival rates. The versican G3 domain is important in breast cancer cell growth, migration and bone metastasis. However, mechanistic studies evaluating versican G3 enhanced breast cancer bone metastasis are limited. Methods A versican G3 construct was exogenously expressed in the 66c14 and the MC3T3-E1 cell line. Cells were observed through light microscopy and viability analyzed by Coulter Counter or determined with colorimetric proliferation assays. The Annexin V-FITC apoptosis detection kit was used to detect apoptotic activity. Modified Chemotactic Boyden chamber migration invasion assays were applied to observe tumor migration and invasion to bone stromal cells and MC3T3-E1 cells. Alkaline phosphatase (ALP staining and ALP ELISA assays were performed to observe ALP activity in MC3T3-E1 cells. Results In the four mouse breast cancer cell lines 67NR, 66c14, 4T07, and 4T1, 4T1 cells expressed higher levels of versican, and showed higher migration and invasion ability to MC3T3-E1 cells and primary bone stromal cells. 4T1 conditioned medium (CM inhibited MC3T3-E1 cell growth, and even lead to apoptosis. Only 4T1 CM prevented MC3T3-E1 cell differentiation, noted by inhibition of alkaline phosphatase (ALP activity. We exogenously expressed a versican G3 construct in a cell line that expresses low versican levels (66c14, and observed that the G3-expressing 66c14 cells showed enhanced cell migration and invasion to bone stromal and MC3T3-E1 cells. This observation was prevented by selective EGFR inhibitor AG1478, selective MEK inhibitor PD 98059, and selective AKT inhibitor Triciribine, but not by selective JNK inhibitor SP 600125. Versican G3 enhanced breast cancer cell invasion to bone stromal cells or osteoblast cells appears to occur through enhancing EGFR/ERK or AKT signaling

  11. Human CD34+ CD133+ hematopoietic stem cells cultured with growth factors including Angptl5 efficiently engraft adult NOD-SCID Il2rγ-/- (NSG mice.

    Directory of Open Access Journals (Sweden)

    Adam C Drake

    Full Text Available Increasing demand for human hematopoietic stem cells (HSCs in clinical and research applications necessitates expansion of HSCs in vitro. Before these cells can be used they must be carefully evaluated to assess their stem cell activity. Here, we expanded cord blood CD34(+ CD133(+ cells in a defined medium containing angiopoietin like 5 and insulin-like growth factor binding protein 2 and evaluated the cells for stem cell activity in NOD-SCID Il2rg(-/- (NSG mice by multi-lineage engraftment, long term reconstitution, limiting dilution and serial reconstitution. The phenotype of expanded cells was characterized by flow cytometry during the course of expansion and following engraftment in mice. We show that the SCID repopulating activity resides in the CD34(+ CD133(+ fraction of expanded cells and that CD34(+ CD133(+ cell number correlates with SCID repopulating activity before and after culture. The expanded cells mediate long-term hematopoiesis and serial reconstitution in NSG mice. Furthermore, they efficiently reconstitute not only neonate but also adult NSG recipients, generating human blood cell populations similar to those reported in mice reconstituted with uncultured human HSCs. These findings suggest an expansion of long term HSCs in our culture and show that expression of CD34 and CD133 serves as a marker for HSC activity in human cord blood cell cultures. The ability to expand human HSCs in vitro should facilitate clinical use of HSCs and large-scale construction of humanized mice from the same donor for research applications.

  12. TAE226, a Bis-Anilino Pyrimidine Compound, Inhibits the EGFR-Mutant Kinase Including T790M Mutant to Show Anti-Tumor Effect on EGFR-Mutant Non-Small Cell Lung Cancer Cells.

    Directory of Open Access Journals (Sweden)

    Hiroki Otani

    Full Text Available TAE226, a bis-anilino pyrimidine compound, has been developed as an inhibitor of focal adhesion kinase (FAK and insulin-like growth factor-I receptor (IGF-IR. In this study, we investigated the effect of TAE226 on non-small-cell lung cancer (NSCLC, especially focusing on the EGFR mutational status. TAE226 was more effective against cells with mutant EGFR, including the T790M mutant, than against cells with wild-type one. TAE226 preferentially inhibited phospho-EGFR and its downstream signaling mediators in the cells with mutant EGFR than in those with wild-type one. Phosphorylation of FAK and IGF-IR was not inhibited at the concentration at which the proliferation of EGFR-mutant cells was inhibited. Results of the in vitro binding assay indicated significant differences in the affinity for TAE226 between the wild-type and L858R (or delE746_A750 mutant, and the reduced affinity of ATP to the L858R (or delE746_A750 mutant resulted in good responsiveness of the L858R (or delE746_A750 mutant cells to TAE226. Of interest, the L858R/T790M or delE746_A750/T790M mutant enhanced the binding affinity for TAE226 compared with the L858R or delE746_A750 mutant, resulting in the effectiveness of TAE226 against T790M mutant cells despite the T790M mutation restoring the ATP affinity for the mutant EGFR close to that for the wild-type. TAE226 also showed higher affinity of about 15-fold for the L858R/T790M mutant than for the wild-type one by kinetic interaction analysis. The anti-tumor effect against EGFR-mutant tumors including T790M mutation was confirmed in mouse models without any significant toxicity. In summary, we showed that TAE226 inhibited the activation of mutant EGFR and exhibited anti-proliferative activity against NSCLCs carrying EGFR mutations, including T790M mutation.

  13. A new staging system for locally advanced (pT3-4) renal cell carcinoma: a multicenter European study including 2,000 patients.

    NARCIS (Netherlands)

    Ficarra, V.; Galfano, A.; Guille, F.; Schips, L.; Tostain, J.; Mejean, A.; Lang, H.; Mulders, P.F.A.; Taille, A. De La; Chautard, D.; Descotes, J.L.; Cindolo, L.; Novara, G.; Rioux-Leclercq, N.; Zattoni, F.; Artibani, W.; Patard, J.J.

    2007-01-01

    PURPOSE: We provide an adequate prognostic stratification for locally advanced renal cell carcinoma and propose a new TNM classification. MATERIALS AND METHODS: We analyzed clinical and pathological data on a large series of patients undergoing radical nephrectomy for pT3-4 renal cell carcinoma at 1

  14. Transformative Dynamics in Detailed Planning

    DEFF Research Database (Denmark)

    Quitzau, Maj-Britt; Poulsen, Naja; Gustavsson, Ted;

    that the translation process relies heavily on integration of impositions in the detailed plan, although this has clear limitations, since some sustainable strategies are more difficult to impose than others. It also shows how strategic navigation may represent an alternative translation strategy to promote more...... difficult sustainable strategies that address the project design more directly. In conclusion, the paper argues that strategic navigation represents a stronger mediator of change compared to the detailed plan, but that especially timing issues in the coordination between formal planning and design processes...

  15. Comparing Epileptiform Behavior of Mesoscale Detailed Models and Population Models of Neocortex

    NARCIS (Netherlands)

    Visser, Sid; Meijer, Hil G.E.; Lee, Hyong C.; Drongelen, van Wim; Putten, van Michel J.A.M; Gils, van Stephan A.

    2010-01-01

    Two models of the neocortex are developed to study normal and pathologic neuronal activity. One model contains a detailed description of a neocortical microcolumn represented by 656 neurons, including superficial and deep pyramidal cells, four types of inhibitory neurons, and realistic synaptic cont

  16. Solar power satellite. Concept evaluation. Activities report. Volume 2: Detailed report

    Science.gov (United States)

    1977-01-01

    Comparative data are presented among various design approaches to thermal engine and photovoltaic SPS (Solar Power System) concepts, to provide criteria for selecting the most promising systems for more detailed definition. The major areas of the SPS system to be examined include solar cells, microwave power transmission, transportation, structure, rectenna, energy payback, resources, and environmental issues.

  17. DAGAL: Detailed Anatomy of Galaxies

    CERN Document Server

    Knapen, Johan H

    2016-01-01

    The current IAU Symposium is closely connected to the EU-funded network DAGAL (Detailed Anatomy of Galaxies), with the final annual network meeting of DAGAL being at the core of this international symposium. In this short paper, we give an overview of DAGAL, its training activities, and some of the scientific advances that have been made under its umbrella.

  18. Fatigue-Prone Details in Steel Bridges

    Directory of Open Access Journals (Sweden)

    Mohsen Heshmati

    2012-11-01

    Full Text Available This paper reviews the results of a comprehensive investigation including more than 100 fatigue damage cases, reported for steel and composite bridges. The damage cases are categorized according to types of detail. The mechanisms behind fatigue damage in each category are identified and studied. It was found that more than 90% of all reported damage cases are of deformation-induced type and generated by some kind of unintentional or otherwise overlooked interaction between different load-carrying members or systems in the bridge. Poor detailing, with unstiffened gaps and abrupt changes in stiffness at the connections between different members were also found to contribute to fatigue cracking in many details.

  19. Progenitor Cell Therapy in a Porcine Acute Myocardial Infarction Model Induces Cardiac Hypertrophy, Mediated by Paracrine Secretion of Cardiotrophic Factors Including TGFβ1

    OpenAIRE

    Doyle, Brendan; Sorajja, Paul; Hynes, Brian; Kumar, Arun H. S.; Araoz, Phillip A.; Stalboerger, Paul G.; Miller, Dylan; Reed, Cynthia; Schmeckpeper, Jeffrey; Wang, Shaohua; Liu, Chunsheng; Terzic, Andre; Kruger, David; Riederer, Stephen; Caplice, Noel M.

    2008-01-01

    Administration of endothelial progenitor cells (EPC) is a promising therapy for post-infarction cardiac repair. However, the mechanisms that underlie apparent beneficial effects on myocardial remodeling are unclear. In a porcine model of acute myocardial infarction, we investigated the therapeutic effects of a mixed population of culture modified peripheral blood mononuclear cells (termed hereafter porcine EPC). Porcine EPC were isolated using methods identical to those previously adopted for...

  20. Phage T4 endonuclease V stimulates DNA repair replication in isolated nuclei from ultraviolet-irradiated human cells, including xeroderma pigmentosum fibroblasts

    International Nuclear Information System (INIS)

    The repair mode of DNA replication has been demonstrated in isolated nuclei from uv-irradiated human cells. Nuclei are incubated in a mixture containing [3H]thymidine triphosphate and bromodeoxyuridine triphosphate in a 1:5 ratio. The 3H at the density of parental DNA in alkaline CsCl density gradients is then a measure of repair. In nuclei prepared from WI38 cells 30 min after irradiation, repair replication is uv-dependent and proceeds at approximately the in vivo rate for 5 min. Repair replication is reduced in irradiated nuclei or in nuclei prepared immediately after irradiation. It is Mg2+-dependent and stimulated by added ATP and deoxyribonucleoside triphosphates. No repair replication is observed in nuclei from xeroderma pigmentosum (complementation group A) cells. However, upon addition of coliphage T4 endonuclease V, which specifically nicks DNA containing pyrimidine dimers, repair replication is observed in nuclei from irradiated xeroderma pigmentosum cells and is stimulated in WI38 nuclei. The reaction then persists for an hour and is dependent upon added ATP and deoxyribonucleoside triphosphates. The repair label is in stretches of roughly 35 nucleotides, as it is in intact cells. Added pancreatic DNase does not promote uv-dependent repair synthesis. Our results support the view that xeroderma pigmentosum (group A) cells are defective in the incision step of the DNA excision repair pathway, and demonstrate the utility of this system for probing DNA repair mechanisms

  1. Progenitor Cell Therapy in a Porcine Acute Myocardial Infarction Model Induces Cardiac Hypertrophy, Mediated by Paracrine Secretion of Cardiotrophic Factors Including TGFβ1

    Science.gov (United States)

    Doyle, Brendan; Sorajja, Paul; Hynes, Brian; Kumar, Arun H.S.; Araoz, Phillip A.; Stalboerger, Paul G.; Miller, Dylan; Reed, Cynthia; Schmeckpeper, Jeffrey; Wang, Shaohua; Liu, Chunsheng; Terzic, Andre; Kruger, David; Riederer, Stephen

    2008-01-01

    Administration of endothelial progenitor cells (EPC) is a promising therapy for post-infarction cardiac repair. However, the mechanisms that underlie apparent beneficial effects on myocardial remodeling are unclear. In a porcine model of acute myocardial infarction, we investigated the therapeutic effects of a mixed population of culture modified peripheral blood mononuclear cells (termed hereafter porcine EPC). Porcine EPC were isolated using methods identical to those previously adopted for harvest of EPC in human cell therapy studies. In addition the therapeutic effects of paracrine factors secreted by these cells was evaluated in vitro and in vivo. Intracoronary injection of autologous porcine EPC was associated with increased infarct territory mass and improved regional ventricular systolic function at 2 months compared to control. Treatment with conditioned media derived from autologous EPC was associated with similar improved effects on infarct territory mass and function. Histologic analysis of the infarct territory revealed significantly increased cardiomyocyte size in EPC and conditioned media treated groups, when compared to controls. A paracrine EPC effect was also verified in a pure myocardial preparation in which cardiomyocytes devoid of fibroblast, neuronal and vascular elements directly responded by increasing cell mass when exposed to the same conditioned media. Analysis of conditioned media revealed elevated levels of TGFβ1 (human 267.3±11.8 pg/ml, porcine 57.1±6.1 pg/ml), a recognized mediator of hypertrophic signaling in the heart. Neutralizing antibodies to TGFβ1 attenuated the pro-hypertrophic effect of conditioned media, and use of recombinant TGFβ1 added to fresh media replicated the pro-hypertrophic effects of conditioned media in vitro. These data demonstrate the potential of paracrine factors secreted from endothelial progenitor cells to induce cardiomyocyte hypertrophy contributing to increased infarct territory LV mass, with

  2. A model predictive control strategy for the space heating of a smart building including cogeneration of a fuel cell-electrolyzer system

    DEFF Research Database (Denmark)

    Sossan, Fabrizio; Bindner, Henrik W.; Madsen, Henrik;

    2014-01-01

    that the predictive energy replacement strategy reduces the operating costs of the system and is able to provide a larger amount of regulating power to the grid. In the paper, we also develop a novel dynamic model of a PEM fuel cell suitable for micro-grid applications. The model is realized applying a grey....... In the proposed setup, heat is provided by conventional electric radiators and a combined heat and power generation system, composed by a fuel cell and an electrolyzer. The energy replacement strategy is formulated using model predictive control and mathematical models of the components involved. Simulations show...

  3. First-line erlotinib and bevacizumab in patients with locally advanced and/or metastatic non-small-cell lung cancer : a phase II study including molecular imaging

    NARCIS (Netherlands)

    Dingemans, A. -M. C.; de Langen, A. J.; van den Boogaart, V.; Marcus, J. T.; Backes, W. H.; Scholtens, H. T. G. M.; van Tinteren, H.; Hoekstra, O. S.; Pruim, J.; Brans, B.; Thunnissen, F. B.; Smit, E. F.; Groen, H. J. M.

    2011-01-01

    Background: Both bevacizumab and erlotinib have clinical activity in non-small-cell lung cancer (NSCLC). Preclinical data suggest synergistic activity. Patients and methods: Chemonaive patients with stage IIIb or IV non-squamous NSCLC were treated with bevacizumab 15 mg/kg every 3 weeks and erlotini

  4. Granular cell tumor of the oral cavity; a case series including a case of metachronous occurrence in the tongue and the lung

    NARCIS (Netherlands)

    S. van de Loo; E. Thunissen; P. Postmus; I. van der Waal

    2015-01-01

    The granular cell tumor (GCT) is a rare, benign tumor that most commonly occurs in the oral cavity, particularly in the anterior part of the tongue. In this study the experience with 16 patients with a GCT observed in a single Institution will be discussed. Although no radicality has been obtained i

  5. SU-D-BRB-06: Treating Glioblastoma Multiforme (GBM) as a Chronic Disease: Implication of Temporal-Spatial Dose Fractionation Optimization Including Cancer Stem Cell Dynamics

    International Nuclear Information System (INIS)

    Purpose: To explore the feasibility of improving GBM treatment outcome with temporal-spatial dose optimization of an ordinary differential equation (ODE) that models the differentiation and distinct radiosensitivity between cancer stem cells (CSC) and differentiated cancer cells (DCC). Methods: The ODE was formulated into a non-convex optimization problem with the objective to minimize remaining total cancer cells 500 days from the onset of radiotherapy when the total cancer cell number was 3.5×107, while maintaining normal tissue biological effective dose (BED) of 100Gy resulted from standard prescription of 2Gyx30. Assuming spatially separated CSC and DCC, optimization was also performed to explore the potential benefit from dose-painting the two compartments. Dose escalation to a sub-cell-population in the GTV was also examined assuming that a 2 cm margin around the GTV allows sufficient dose drop-off to 100Gy BED. The recurrence time was determined as the time at which the total cancer cell number regrows to 109 cells. Results: The recurrence time with variable fractional doses administered once per week, bi-week and month for one year were found to be 615, 593 and 570 days, superior to the standard-prescription recurrence time of 418 days. The optimal dose-fraction size progression for both uniform and dose-painting to the tumor is low and relatively constant in the beginning and gradually increases to more aggressive fractions at end of the treatment course. Dose escalation to BED of 200Gy to the whole tumor alongside with protracted weekly treatment was found to further delay recurrence to 733 days. Dose-painting of 200 and 500Gy BED to CSC on a year-long weekly schedule further extended recurrence to 736 and 1076 days, respectively. Conclusion: GBM treatment outcome can possibly be improved with a chronic treatment approach. Further dose escalation to the entire tumor or CSC targeted killing is needed to achieve total tumor control. This work is supported

  6. SU-D-BRB-06: Treating Glioblastoma Multiforme (GBM) as a Chronic Disease: Implication of Temporal-Spatial Dose Fractionation Optimization Including Cancer Stem Cell Dynamics

    Energy Technology Data Exchange (ETDEWEB)

    Yu, V; Nguyen, D; Pajonk, F; Kaprealian, T; Kupelian, P; Steinberg, M; Low, D; Sheng, K [Department of Radiation Oncology, UCLA, Los Angeles, CA (United States)

    2015-06-15

    Purpose: To explore the feasibility of improving GBM treatment outcome with temporal-spatial dose optimization of an ordinary differential equation (ODE) that models the differentiation and distinct radiosensitivity between cancer stem cells (CSC) and differentiated cancer cells (DCC). Methods: The ODE was formulated into a non-convex optimization problem with the objective to minimize remaining total cancer cells 500 days from the onset of radiotherapy when the total cancer cell number was 3.5×10{sup 7}, while maintaining normal tissue biological effective dose (BED) of 100Gy resulted from standard prescription of 2Gyx30. Assuming spatially separated CSC and DCC, optimization was also performed to explore the potential benefit from dose-painting the two compartments. Dose escalation to a sub-cell-population in the GTV was also examined assuming that a 2 cm margin around the GTV allows sufficient dose drop-off to 100Gy BED. The recurrence time was determined as the time at which the total cancer cell number regrows to 10{sup 9} cells. Results: The recurrence time with variable fractional doses administered once per week, bi-week and month for one year were found to be 615, 593 and 570 days, superior to the standard-prescription recurrence time of 418 days. The optimal dose-fraction size progression for both uniform and dose-painting to the tumor is low and relatively constant in the beginning and gradually increases to more aggressive fractions at end of the treatment course. Dose escalation to BED of 200Gy to the whole tumor alongside with protracted weekly treatment was found to further delay recurrence to 733 days. Dose-painting of 200 and 500Gy BED to CSC on a year-long weekly schedule further extended recurrence to 736 and 1076 days, respectively. Conclusion: GBM treatment outcome can possibly be improved with a chronic treatment approach. Further dose escalation to the entire tumor or CSC targeted killing is needed to achieve total tumor control. This work

  7. Fatigue-Prone Details in Steel Bridges

    OpenAIRE

    Mohsen Heshmati; Mohammad Al-Emrani; Reza Haghani

    2012-01-01

    This paper reviews the results of a comprehensive investigation including more than 100 fatigue damage cases, reported for steel and composite bridges. The damage cases are categorized according to types of detail. The mechanisms behind fatigue damage in each category are identified and studied. It was found that more than 90% of all reported damage cases are of deformation-induced type and generated by some kind of unintentional or otherwise overlooked interaction between different load-carr...

  8. Etiology of out-of-hospital cardiac arrest diagnosed via detailed examinations including perimortem computed tomography

    Directory of Open Access Journals (Sweden)

    Yoshihiro Moriwaki

    2013-01-01

    Full Text Available Context: The spectrum of the etiology of out-of-hospital cardiopulmonary arrest (OHCPA has not been established. We have performed perimortem computed tomography (CT during cardiopulmonary resuscitation. Aims: To clarify the incidence of non-cardiac etiology (NCE, actual distribution of the causes of OHCPA via perimortem CT and its usefulness. Settings and Design: Population-based observational case series study. Materials and Methods: We reviewed the medical records of 1846 consecutive OHCPA cases and divided them into two groups: 370 showing an obvious cause of OHCPA with NCE (trauma, neck hanging, terminal stage of malignancy, and gastrointestinal bleeding and others. Results: Of a total OHCPA, perimortem CT was performed in 57.5% and 62.5% were finally diagnosed as NCE: Acute aortic dissection (AAD 8.07%, pulmonary thrombo-embolization (PTE 1.46%, hypoxia due to pneumonia 5.25%, asthma and acute worsening of chronic obstructive pulmonary disease 2.06%, cerebrovascular disorder (CVD 4.48%, airway obstruction 7.64%, and submersion 5.63%. The rates of patients who survived to hospital discharge were 6-14% in patients with NCE. Out of the 1476 cases excluding obvious NCE of OHCPA, 66.3% underwent perimortem CT, 14.6% of cases without obvious NCE and 22.1% of cases with perimortem CT were confirmed as having some NCE. Conclusions: Of the total OHCPA the incidences of NCE was 62.5%; the leading etiologies were AAD, airway obstruction, submersion, hypoxia and CVD. The rates of cases converted from cardiac etiology to NCE using perimortem CT were 14.6% of cases without an obvious NCE.

  9. Aircraft empennage structural detail design

    Science.gov (United States)

    Meholic, Greg; Brown, Rhonda; Hall, Melissa; Harvey, Robert; Singer, Michael; Tella, Gustavo

    1993-01-01

    This project involved the detailed design of the aft fuselage and empennage structure, vertical stabilizer, rudder, horizontal stabilizer, and elevator for the Triton primary flight trainer. The main design goals under consideration were to illustrate the integration of the control systems devices used in the tail surfaces and their necessary structural supports as well as the elevator trim, navigational lighting system, electrical systems, tail-located ground tie, and fuselage/cabin interface structure. Accommodations for maintenance, lubrication, adjustment, and repairability were devised. Weight, fabrication, and (sub)assembly goals were addressed. All designs were in accordance with the FAR Part 23 stipulations for a normal category aircraft.

  10. Seasonal Variation in Abundance and Species Composition of a Planktonic Diatom Assemblage Including Viable Cells on the Bottom in Matsushima Bay, Northeastern Japan

    Institute of Scientific and Technical Information of China (English)

    ICHINOMIYA Mutsuo; TANIGUCHI Akira

    2003-01-01

    Seasonal variation in abundance and species composition of a planktonic diatom assemblage distributed in the water column and also settled on the bottom was investigated for the shallow coastal water in Matsushima Bay on the Pacific coast of northeastern Japan during the period from October 1999 to September 2000. A spring bloom of diatoms began in April when nutrient concentrations started to increase, indicating the importance of nutrients. Viable cells of Skeletonema costatum and Thalassiosira spp., which were the dominant species in the water column throughout the year, were also always abundant in the bottom sediment. Both populations in the water column and on the bottom fluctuated essentially in parallel.For the planktonic diatoms in shallow coastal waters to maintain their vegetative populations in the water column, it would be advantageous for them to have a seeding population of viable cells on the bottom that are easily resuspended into the upper photic layer.

  11. Insulin-like growth factor I induces proliferation and migration of porcine trophectoderm cells through multiple cell signaling pathways, including protooncogenic protein kinase 1 and mitogen-activated protein kinase.

    Science.gov (United States)

    Jeong, Wooyoung; Song, Gwonhwa; Bazer, Fuller W; Kim, Jinyoung

    2014-03-25

    , IGF-I significantly stimulated both proliferation and migration of pTr cells, but these effects were blocked by P38 inhibitor (SB203580), U0126, MTOR inhibitor (rapamycin) and LY294002. Taken together, these results indicate that IGF-I coordinately regulates multiple cell signaling pathways including PI3K-AKT1-RPS6 and ERK1/2 MAPK signaling pathways that are critical to proliferation, migration and survival of trophectoderm cells during early pregnancy in pigs. PMID:24508636

  12. A systems-level approach to parental genomic imprinting: the imprinted gene network includes extracellular matrix genes and regulates cell cycle exit and differentiation

    OpenAIRE

    Al Adhami, Hala; Evano, Brendan; Le Digarcher, Anne; Gueydan, Charlotte; Dubois, Emeric; Parrinello, Hugues; Dantec, Christelle; Bouschet, Tristan; Varrault, Annie; Journot, Laurent

    2015-01-01

    Genomic imprinting is an epigenetic mechanism that restrains the expression of ∼100 eutherian genes in a parent-of-origin-specific manner. The reason for this selective targeting of genes with seemingly disparate molecular functions is unclear. In the present work, we show that imprinted genes are coexpressed in a network that is regulated at the transition from proliferation to quiescence and differentiation during fibroblast cell cycle withdrawal, adipogenesis in vitro, and ...

  13. Modification of histones by sugar β-N-acetylglucosamine (GlcNAc) occurs on multiple residues, including histone H3 serine 10, and is cell cycle-regulated.

    Science.gov (United States)

    Zhang, Suisheng; Roche, Kevin; Nasheuer, Heinz-Peter; Lowndes, Noel Francis

    2011-10-28

    The monosaccharide, β-N-acetylglucosamine (GlcNAc), can be added to the hydroxyl group of either serines or threonines to generate an O-linked β-N-acetylglucosamine (O-GlcNAc) residue (Love, D. C., and Hanover, J. A. (2005) Sci. STKE 2005 312, 1-14; Hart, G. W., Housley, M. P., and Slawson, C. (2007) Nature 446, 1017-1022). This post-translational protein modification, termed O-GlcNAcylation, is reversible, analogous to phosphorylation, and has been implicated in many cellular processes. Here, we present evidence that in human cells all four core histones of the nucleosome are substrates for this glycosylation in the relative abundance H3, H4/H2B, and H2A. Increasing the intracellular level of UDP-GlcNAc, the nucleotide sugar donor substrate for O-GlcNAcylation enhanced histone O-GlcNAcylation and partially suppressed phosphorylation of histone H3 at serine 10 (H3S10ph). Expression of recombinant H3.3 harboring an S10A mutation abrogated histone H3 O-GlcNAcylation relative to its wild-type version, consistent with H3S10 being a site of histone O-GlcNAcylation (H3S10glc). Moreover, O-GlcNAcylated histones were lost from H3S10ph immunoprecipitates, whereas immunoprecipitation of either H3K4me3 or H3K9me3 (active or inactive histone marks, respectively) resulted in co-immunoprecipitation of O-GlcNAcylated histones. We also examined histone O-GlcNAcylation during cell cycle progression. Histone O-GlcNAcylation is high in G(1) cells, declines throughout the S phase, increases again during late S/early G(2), and persists through late G(2) and mitosis. Thus, O-GlcNAcylation is a novel histone post-translational modification regulating chromatin conformation during transcription and cell cycle progression.

  14. Impact of plasmids, including those encodingVirB4/D4 type IV secretion systems, on Salmonella enterica serovar Heidelberg virulence in macrophages and epithelial cells.

    Directory of Open Access Journals (Sweden)

    Kuppan Gokulan

    Full Text Available Salmonella enterica serovar Heidelberg (S. Heidelberg can cause foodborne illness in humans following the consumption of contaminated meat and poultry products. Recent studies from our laboratory have demonstrated that certain S. Heidelberg isolated from food-animal sources harbor multiple transmissible plasmids with genes that encode antimicrobial resistance, virulence and a VirB4/D4 type-IV secretion system. This study examines the potential role of these transmissible plasmids in bacterial uptake and survival in intestinal epithelial cells and macrophages, and the molecular basis of host immune system modulation that may be associated with disease progression. A series of transconjugant and transformant strains were developed with different combinations of the plasmids to determine the roles of the individual and combinations of plasmids on virulence. Overall the Salmonella strains containing the VirB/D4 T4SS plasmids entered and survived in epithelial cells and macrophages to a greater degree than those without the plasmid, even though they carried other plasmid types. During entry in macrophages, the VirB/D4 T4SS encoding genes are up-regulated in a time-dependent fashion. When the potential mechanisms for increased virulence were examined using an antibacterial Response PCR Array, the strain containing the T4SS down regulated several host innate immune response genes which likely contributed to the increased uptake and survival within macrophages and epithelial cells.

  15. Impact of plasmids, including those encodingVirB4/D4 type IV secretion systems, on Salmonella enterica serovar Heidelberg virulence in macrophages and epithelial cells.

    Science.gov (United States)

    Gokulan, Kuppan; Khare, Sangeeta; Rooney, Anthony W; Han, Jing; Lynne, Aaron M; Foley, Steven L

    2013-01-01

    Salmonella enterica serovar Heidelberg (S. Heidelberg) can cause foodborne illness in humans following the consumption of contaminated meat and poultry products. Recent studies from our laboratory have demonstrated that certain S. Heidelberg isolated from food-animal sources harbor multiple transmissible plasmids with genes that encode antimicrobial resistance, virulence and a VirB4/D4 type-IV secretion system. This study examines the potential role of these transmissible plasmids in bacterial uptake and survival in intestinal epithelial cells and macrophages, and the molecular basis of host immune system modulation that may be associated with disease progression. A series of transconjugant and transformant strains were developed with different combinations of the plasmids to determine the roles of the individual and combinations of plasmids on virulence. Overall the Salmonella strains containing the VirB/D4 T4SS plasmids entered and survived in epithelial cells and macrophages to a greater degree than those without the plasmid, even though they carried other plasmid types. During entry in macrophages, the VirB/D4 T4SS encoding genes are up-regulated in a time-dependent fashion. When the potential mechanisms for increased virulence were examined using an antibacterial Response PCR Array, the strain containing the T4SS down regulated several host innate immune response genes which likely contributed to the increased uptake and survival within macrophages and epithelial cells.

  16. Characterization of Genotoxic Response to 15 Multiwalled Carbon Nanotubes with Variable Physicochemical Properties Including Surface Functionalizations in the FE1-Muta(TM) Mouse Lung Epithelial Cell Line

    DEFF Research Database (Denmark)

    Jackson, Petra; Kling, Kirsten; Jensen, Keld Alstrup;

    2015-01-01

    Carbon nanotubes vary greatly in physicochemical properties. We compared cytotoxic and genotoxic response to 15 multiwalled carbon nanotubes (MWCNT) with varying physicochemical properties to identify drivers of toxic responses. The studied MWCNT included OECD Working Party on Manufactured...

  17. Enteric Bacterial Metabolites Propionic and Butyric Acid Modulate Gene Expression, Including CREB-Dependent Catecholaminergic Neurotransmission, in PC12 Cells - Possible Relevance to Autism Spectrum Disorders

    OpenAIRE

    Nankova, Bistra B; Agarwal, Raj; MacFabe, Derrick F.; La Gamma, Edmund F.

    2014-01-01

    Alterations in gut microbiome composition have an emerging role in health and disease including brain function and behavior. Short chain fatty acids (SCFA) like propionic (PPA), and butyric acid (BA), which are present in diet and are fermentation products of many gastrointestinal bacteria, are showing increasing importance in host health, but also may be environmental contributors in neurodevelopmental disorders including autism spectrum disorders (ASD). Further to this we have shown SCFA ad...

  18. Endothelin-2/Vasoactive Intestinal Contractor: Regulation of Expression via Reactive Oxygen Species Induced by CoCl22, and Biological Activities Including Neurite Outgrowth in PC12 Cells

    Directory of Open Access Journals (Sweden)

    Eiichi Kotake-Nara

    2006-01-01

    Full Text Available This paper reviews the local hormone endothelin-2 (ET-2, or vasoactive intestinal contractor (VIC, a member of the vasoconstrictor ET peptide family, where ET-2 is the human orthologous peptide of the murine VIC. While ET-2/VIC gene expression has been observed in some normal tissues, ET-2 recently has been reported to act as a tumor marker and as a hypoxia-induced autocrine survival factor in tumor cells. A recently published study reported that the hypoxic mimetic agent CoCl2 at 200 µM increased expression of the ET-2/VIC gene, decreased expression of the ET-1 gene, and induced intracellular reactive oxygen species (ROS increase and neurite outgrowth in neuronal model PC12 cells. The ROS was generated by addition of CoCl2 to the culture medium, and the CoCl2-induced effects were completely inhibited by the antioxidant N-acetyl cysteine. Furthermore, interleukin-6 (IL-6 gene expression was up-regulated upon the differentiation induced by CoCl2. These results suggest that expression of ET-2/VIC and ET-1 mediated by CoCl2-induced ROS may be associated with neuronal differentiation through the regulation of IL-6 expression. CoCl2 acts as a pro-oxidant, as do Fe(II, III and Cu(II. However, some biological activities have been reported for CoCl2 that have not been observed for other metal salts such as FeCl3, CuSO4, and NiCl2. The characteristic actions of CoCl2 may be associated with the differentiation of PC12 cells. Further elucidation of the mechanism of neurite outgrowth and regulation of ET-2/VIC expression by CoCl2 may lead to the development of treatments for neuronal disorders.

  19. Peripheral nerve pathology, including aberrant Schwann cell differentiation, is ameliorated by doxycycline in a laminin-α2-deficient mouse model of congenital muscular dystrophy

    OpenAIRE

    Homma, Sachiko; Beermann, Mary Lou; Miller, Jeffrey Boone

    2011-01-01

    The most common form of childhood congenital muscular dystrophy, Type 1A (MDC1A), is caused by mutations in the human LAMA2 gene that encodes the laminin-α2 subunit. In addition to skeletal muscle deficits, MDC1A patients typically show a loss of peripheral nerve function. To identify the mechanisms underlying this loss of nerve function, we have examined pathology and cell differentiation in sciatic nerves and ventral roots of the laminin-α2-deficient (Lama2−/−) mice, which are models for MD...

  20. An atypical human induced pluripotent stem cell line with a complex, stable, and balanced genomic rearrangement including a large de novo 1q uniparental disomy.

    Science.gov (United States)

    Steichen, Clara; Maluenda, Jérôme; Tosca, Lucie; Luce, Eléanor; Pineau, Dominique; Dianat, Noushin; Hannoun, Zara; Tachdjian, Gérard; Melki, Judith; Dubart-Kupperschmitt, Anne

    2015-03-01

    Human induced pluripotent stem cells (hiPSCs) hold great promise for cell therapy through their use as vital tools for regenerative and personalized medicine. However, the genomic integrity of hiPSCs still raises some concern and is one of the barriers limiting their use in clinical applications. Numerous articles have reported the occurrence of aneuploidies, copy number variations, or single point mutations in hiPSCs, and nonintegrative reprogramming strategies have been developed to minimize the impact of the reprogramming process on the hiPSC genome. Here, we report the characterization of an hiPSC line generated by daily transfections of modified messenger RNAs, displaying several genomic abnormalities. Karyotype analysis showed a complex genomic rearrangement, which remained stable during long-term culture. Fluorescent in situ hybridization analyses were performed on the hiPSC line showing that this karyotype is balanced. Interestingly, single-nucleotide polymorphism analysis revealed the presence of a large 1q region of uniparental disomy (UPD), demonstrating for the first time that UPD can occur in a noncompensatory context during nonintegrative reprogramming of normal fibroblasts.

  1. Hematopoietic stem cell transplantation activity worldwide in 2012 and a SWOT analysis of the Worldwide Network for Blood and Marrow Transplantation Group including the global survey.

    Science.gov (United States)

    Niederwieser, D; Baldomero, H; Szer, J; Gratwohl, M; Aljurf, M; Atsuta, Y; Bouzas, L F; Confer, D; Greinix, H; Horowitz, M; Iida, M; Lipton, J; Mohty, M; Novitzky, N; Nunez, J; Passweg, J; Pasquini, M C; Kodera, Y; Apperley, J; Seber, A; Gratwohl, A

    2016-06-01

    Data on 68 146 hematopoietic stem cell transplants (HSCTs) (53% autologous and 47% allogeneic) gathered by 1566 teams from 77 countries and reported through their regional transplant organizations were analyzed by main indication, donor type and stem cell source for the year 2012. With transplant rates ranging from 0.1 to 1001 per 10 million inhabitants, more HSCTs were registered from unrelated 16 433 donors than related 15 493 donors. Grafts were collected from peripheral blood (66%), bone marrow (24%; mainly non-malignant disorders) and cord blood (10%). Compared with 2006, an increase of 46% total (57% allogeneic and 38% autologous) was observed. Growth was due to an increase in reporting teams (18%) and median transplant activity/team (from 38 to 48 HSCTs/team). An increase of 167% was noted in mismatched/haploidentical family HSCT. A Strengths, Weaknesses, Opportunities, Threats (SWOT) analysis revealed the global perspective of WBMT to be its major strength and identified potential to be the key professional body for patients and authorities. The limited data collection remains its major weakness and threat. In conclusion, global HSCT grows over the years without plateauing (allogeneic>autologous) and at different rates in the four World Health Organization regions. Major increases were observed in allogeneic, haploidentical HSCT and, to a lesser extent, in cord blood transplantation. PMID:26901703

  2. Hematopoietic stem cell transplantation activity worldwide in 2012 and a SWOT analysis of the Worldwide Network for Blood and Marrow Transplantation Group including the global survey.

    Science.gov (United States)

    Niederwieser, D; Baldomero, H; Szer, J; Gratwohl, M; Aljurf, M; Atsuta, Y; Bouzas, L F; Confer, D; Greinix, H; Horowitz, M; Iida, M; Lipton, J; Mohty, M; Novitzky, N; Nunez, J; Passweg, J; Pasquini, M C; Kodera, Y; Apperley, J; Seber, A; Gratwohl, A

    2016-06-01

    Data on 68 146 hematopoietic stem cell transplants (HSCTs) (53% autologous and 47% allogeneic) gathered by 1566 teams from 77 countries and reported through their regional transplant organizations were analyzed by main indication, donor type and stem cell source for the year 2012. With transplant rates ranging from 0.1 to 1001 per 10 million inhabitants, more HSCTs were registered from unrelated 16 433 donors than related 15 493 donors. Grafts were collected from peripheral blood (66%), bone marrow (24%; mainly non-malignant disorders) and cord blood (10%). Compared with 2006, an increase of 46% total (57% allogeneic and 38% autologous) was observed. Growth was due to an increase in reporting teams (18%) and median transplant activity/team (from 38 to 48 HSCTs/team). An increase of 167% was noted in mismatched/haploidentical family HSCT. A Strengths, Weaknesses, Opportunities, Threats (SWOT) analysis revealed the global perspective of WBMT to be its major strength and identified potential to be the key professional body for patients and authorities. The limited data collection remains its major weakness and threat. In conclusion, global HSCT grows over the years without plateauing (allogeneic>autologous) and at different rates in the four World Health Organization regions. Major increases were observed in allogeneic, haploidentical HSCT and, to a lesser extent, in cord blood transplantation.

  3. A Generalized Detailed Balance Relation

    Science.gov (United States)

    Ruelle, David

    2016-08-01

    Given a system M in a thermal bath we obtain a generalized detailed balance relation for the ratio r=π _τ (K→ J)/π _τ (J→ K) of the transition probabilities M:J→ K and M:K→ J in time τ . We assume an active bath, containing solute molecules in metastable states. These molecules may react with M and the transition J→ K occurs through different channels α involving different reactions with the bath. We find that r=sum p^α r^α , where p^α is the probability that channel α occurs, and r^α depends on the amount of heat (more precisely enthalpy) released to the bath in channel α.

  4. A generalized detailed balance relation

    CERN Document Server

    Ruelle, David

    2015-01-01

    Given a system $M$ in a thermal bath we obtain a generalized detailed balance relation for the ratio $r=\\pi_\\tau(K\\to J)/\\pi_\\tau(J\\to K)$ of the transition probabilities $M:J\\to K$ and $M:K\\to J$ in time $\\tau$. We assume an active bath, containing solute molecules in metastable states. These molecules may react with $M$ and the transition $J\\to K$ occurs through different channels $\\alpha$ involving different reactions with the bath. We find that $r=\\sum p^\\alpha r^\\alpha$, where $p^\\alpha$ is the probability that channel $\\alpha$ occurs, and $r^\\alpha$ depends on the amount of heat (more precisely enthalpy) released to the bath in channel $\\alpha$.

  5. Transfection of an expressive construct including IgG1 and Fv1 genes in ovary cell line for infliximab expression

    Directory of Open Access Journals (Sweden)

    Zohreh Sarabinejad

    2016-04-01

    Full Text Available Background: Infeliximab is a form of chimeric antibody which neutralizes the most important inflammatory cytokine, TNF-a, in inflammatory disorders. The aim of current study was to pilot expression of chimeric infliximab in Chinese Hamster ovary (CHO cells. Methods: In this research study, pVITRO2-neo-mcs vector that consist of infliximab light chain and heavy chain was used to transform into the E.coli by CaCl2 method. The plasmid was then purified and transfected to cultured CHO cells by Lipofectamine 2000® (Invitrogen GmbH, Germany. Transfected cells were selected upon G-418 treatment after 2 weeks and the level of expression, based on standard curve, was measured using IgG ELISA kit after 48 hours for each clone. High level expressed clone was then cultured in roller bottles and recombinant chimeric product was purified by protein A affinity chromatography. The purity of the product was analyzed by 10% gel SDS-PAGE from eluted samples. The efficacy of the purification was analyzed by ELISA before and after purification step. This article is a master's student thesis from February 2015 to August 2016 in pharmaceutical technology development center, Tehran University of Medical Sciences, Tehran, Iran. Results: The purified plasmid was analyzed on 2% agarose gel. After selective pressure of G-418, 10 stable transfect clones were assessed for infliximab secretion by IgG ELISA kit at 450 nm. The maximum and minimum expression which detected by ELISA were 23 ng/ml and 6 ng/ml, respectively. The band width of infliximab fraction during purification procedure was observed at 0.7-0.8 min. The efficiency of the purification by ELISA was 70%. On SDS-PAGE analysis, two bands, 25 and 50 kDa, respect to light and heavy chains of Infliximab, was confirmed the expression of recombinant protein. Conclusion: In the current study, the construct for infliximab monoclonal antibody production was designed using genetic engineering techniques and the expression

  6. Thirty Meter Telescope Detailed Science Case: 2015

    CERN Document Server

    Skidmore, Warren; Fukugawa, Misato; Goswami, Aruna; Hao, Lei; Jewitt, David; Laughlin, Greg; Steidel, Charles; Hickson, Paul; Simard, Luc; Schöck, Matthias; Treu, Tommaso; Cohen, Judith; Anupama, G C; Dickinson, Mark; Harrison, Fiona; Kodama, Tadayuki; Lu, Jessica R; Macintosh, Bruce; Malkan, Matt; Mao, Shude; Narita, Norio; Sekiguchi, Tomohiko; Subramaniam, Annapurni; Tanaka, Masaomi; Tian, Feng; A'Hearn, Michael; Akiyama, Masayuki; Ali, Babar; Aoki, Wako; Bagchi, Manjari; Barth, Aaron; Bhalerao, Varun; Bradac, Marusa; Bullock, James; Burgasser, Adam J; Chapman, Scott; Chary, Ranga-Ram; Chiba, Masashi; Cooray, Asantha; Crossfield, Ian; Currie, Thayne; Das, Mousumi; Dewangan, G C; de Grijs, Richard; Do, Tuan; Dong, Subo; Evslin, Jarah; Fang, Taotao; Fang, Xuan; Fassnacht, Christopher; Fletcher, Leigh; Gaidos, Eric; Gal, Roy; Ghez, Andrea; Giavalisco, Mauro; Grady, Carol A; Greathouse, Thomas; Gogoi, Rupjyoti; Guhathakurta, Puragra; Ho, Luis; Hasan, Priya; Herczeg, Gregory J; Honda, Mitsuhiko; Imanishi, Masa; Inanmi, Hanae; Iye, Masanori; Kamath, U S; Kane, Stephen; Kashikawa, Nobunari; Kasliwal, Mansi; Kirby, Vishal KasliwalEvan; Konopacky, Quinn M; Lepine, Sebastien; Li, Di; Li, Jianyang; Liu, Junjun; Liu, Michael C; Lopez-Rodriguez, Enrigue; Lotz, Jennifer; Lubin, Philip; Macri, Lucas; Maeda, Keiichi; Marchis, Franck; Marois, Christian; Marscher, Alan; Martin, Crystal; Matsuo, Taro; Max, Claire; McConnachie, Alan; McGough, Stacy; Melis, Carl; Meyer, Leo; Mumma, Michael; Muto, Takayuki; Nagao, Tohru; Najita, Joan R; Navarro, Julio; Pierce, Michael; Prochaska, Jason X; Oguri, Masamune; Ojha, Devendra K; Okamoto, Yoshiko K; Orton, Glenn; Otarola, Angel; Ouchi, Masami; Packham, Chris; Padgett, Deborah L; Pandey, Shashi Bhushan; Pilachowsky, Catherine; Pontoppidan, Klaus M; Primack, Joel; Puthiyaveettil, Shalima; Ramirez-Ruiz, Enrico; Reddy, Naveen; Rich, Michael; Richter, Matthew J; Schombert, James; Sen, Anjan Ananda; Shi, Jianrong; Sheth, Kartik; Srianand, R; Tan, Jonathan C; Tanaka, Masayuki; Tanner, Angelle; Tominaga, Nozomu; Tytler, David; U, Vivian; Wang, Lingzhi; Wang, Xiaofeng; Wang, Yiping; Wilson, Gillian; Wright, Shelley; Wu, Chao; Wu, Xufeng; Xu, Renxin; Yamada, Toru; Yang, Bin; Zhao, Gongbo; Zhao, Hongsheng

    2015-01-01

    The TMT Detailed Science Case describes the transformational science that the Thirty Meter Telescope will enable. Planned to begin science operations in 2024, TMT will open up opportunities for revolutionary discoveries in essentially every field of astronomy, astrophysics and cosmology, seeing much fainter objects much more clearly than existing telescopes. Per this capability, TMT's science agenda fills all of space and time, from nearby comets and asteroids, to exoplanets, to the most distant galaxies, and all the way back to the very first sources of light in the Universe. More than 150 astronomers from within the TMT partnership and beyond offered input in compiling the new 2015 Detailed Science Case. The contributing astronomers represent the entire TMT partnership, including the California Institute of Technology (Caltech), the Indian Institute of Astrophysics (IIA), the National Astronomical Observatories of the Chinese Academy of Sciences (NAOC), the National Astronomical Observatory of Japan (NAOJ),...

  7. Langevin dynamics neglecting detailed balance condition.

    Science.gov (United States)

    Ohzeki, Masayuki; Ichiki, Akihisa

    2015-07-01

    An improved method for driving a system into a desired distribution, for example, the Gibbs-Boltzmann distribution, is proposed, which makes use of an artificial relaxation process. The standard techniques for achieving the Gibbs-Boltzmann distribution involve numerical simulations under the detailed balance condition. In contrast, in the present study we formulate the Langevin dynamics, for which the corresponding Fokker-Planck operator includes an asymmetric component violating the detailed balance condition. This leads to shifts in the eigenvalues and results in the acceleration of the relaxation toward the steady state. The numerical implementation demonstrates faster convergence and shorter correlation time, and the technique of biased event sampling, Nemoto-Sasa theory, further highlights the efficacy of our method. PMID:26274123

  8. Dutchess Co, NY, Detailed Hydraulics

    Data.gov (United States)

    Federal Emergency Management Agency, Department of Homeland Security — Hydraulic data include spatial datasets and data tables necessary for documenting the hydraulic procedures for estimating flood discharges for a flood insurance...

  9. Methods for rearing Mesostoma ehrenbergii in the laboratory for cell biology experiments, including identification of factors that influence production of different egg types.

    Science.gov (United States)

    Hoang, Carina; Ferraro-Gideon, Jessica; Gauthier, Kimberley; Forer, Arthur

    2013-10-01

    Mesostoma ehrenbergii spermatocytes are uniquely useful to study various aspects of cell division. Their chromosomes are large in size and few in number, with only three bivalent and four univalent chromosomes. During prometaphase, bipolar bivalents oscillate regularly to and from the poles for 1-2 hours. The univalents remain at the poles but occasionally move from one pole to the other. In addition, a precocious cleavage furrow forms during prometaphase and remains partially constricted until anaphase. Attempts to rear these animals indefinitely in laboratory conditions, however, have been mostly unsuccessful because of their reproductive strategy. M. ehrenbergii are hermaphroditic flatworms that can produce viviparous offspring (termed S eggs) and/or diapausing eggs (termed D eggs) and they follow either one of two reproductive patterns: (1) they first form S eggs and following the delivery of these eggs produce D eggs, or (2) they only produce D eggs. When only D eggs are formed, which is common under laboratory conditions, the stocks die out until the D eggs hatch, which is irregular and creates unpredictable wait times. Consequently, in order to maintain M. ehrenbergii stocks to study their spermatocytes, we examined various factors that might influence egg-type production. Feeding them daily and keeping them at 25°C favours S egg production. Currently, our cultures have reached the 53rd generation. We herein describe our rearing and dissection methods, and some experiments which led to our present rearing methods. PMID:23686667

  10. Radiochemotherapy including cisplatin alone versus cisplatin + 5-fluorouracil for locally advanced unresectable stage IV squamous cell carcinoma of the head and neck

    Energy Technology Data Exchange (ETDEWEB)

    Tribius, Silke; Kilic, Yasemin [Dept. of Radiation Oncology, Univ. Medical Center Hamburg-Eppendorf, Hamburg (Germany); Kronemann, Stefanie [Dept. of Radiation Oncology, Univ. Hospital Schleswig-Holstein, Campus Luebeck (Germany); Schroeder, Ursula [Dept. of Head and Neck Surgery, Univ. Hospital Schleswig-Holstein, Campus Luebeck (Germany); Hakim, Samer [Dept. of Oro-Maxillo-Facial Surgery, Univ. Hospital Schleswig-Holstein, Campus Luebeck (Germany); Schild, Steven E. [Dept. of Radiation Oncology, Mayo Clinic, Scottsdale, AZ (United States); Rades, Dirk [Dept. of Radiation Oncology, Univ. Medical Center Hamburg-Eppendorf, Hamburg (Germany); Dept. of Radiation Oncology, Univ. Hospital Schleswig-Holstein, Campus Luebeck (Germany)

    2009-10-15

    Background and purpose: the optimal radiochemotherapy regimen for advanced head-and-neck cancer is still debated. This nonrandomized study compares two cisplatin-based radiochemotherapy regimens in 128 patients with locally advanced unresectable stage IV squamous cell carcinoma of the head and neck (SCCHN). Patients and methods: concurrent chemotherapy consisted of either two courses cisplatin (20 mg/m{sup 2}/d1-5 + 29-33; n = 54) or two courses cisplatin (20 mg/m{sup 2}/d1-5 + 29-33) + 5-fluorouracil (5-FU; 600 mg/m{sup 2}/d1-5 + 29-33; n = 74). Results: at least one grade 3 toxicity occurred in 25 of 54 patients (46%) receiving cisplatin alone and in 52 of 74 patients (70%) receiving cisplatin + 5-FU. The latter regimen was particularly associated with increased rates of mucositis (p = 0.027) and acute skin toxicity (p = 0.001). Seven of 54 (13%) and 20 of 74 patients (27%) received only one chemotherapy course due to treatment-related acute toxicity. Late toxicity in terms of xerostomia, neck fibrosis, skin toxicity, and lymphedema was not significantly different. The 2-year locoregional control rates were 67% after cisplatin alone and 52% after cisplatin + 5-FU (p = 0.35). The metastases-free survival rates were 79% and 69%, respectively (p = 0.65), and the overall survival rates 70% and 51%, respectively (p = 0.10). On multivariate analysis, outcome was significantly associated with performance status, T-category, N-category, hemoglobin level prior to radiotherapy, and radiotherapy break > 1 week. Conclusion: two courses of fractionated cisplatin (20 mg/m{sup 2}/day) alone appear preferable, as this regimen resulted in similar outcome and late toxicity as two courses of cisplatin + 5-FU, but in significantly less acute toxicity. (orig.)

  11. Radiochemotherapy including cisplatin alone versus cisplatin + 5-fluorouracil for locally advanced unresectable stage IV squamous cell carcinoma of the head and neck

    International Nuclear Information System (INIS)

    Background and purpose: the optimal radiochemotherapy regimen for advanced head-and-neck cancer is still debated. This nonrandomized study compares two cisplatin-based radiochemotherapy regimens in 128 patients with locally advanced unresectable stage IV squamous cell carcinoma of the head and neck (SCCHN). Patients and methods: concurrent chemotherapy consisted of either two courses cisplatin (20 mg/m2/d1-5 + 29-33; n = 54) or two courses cisplatin (20 mg/m2/d1-5 + 29-33) + 5-fluorouracil (5-FU; 600 mg/m2/d1-5 + 29-33; n = 74). Results: at least one grade 3 toxicity occurred in 25 of 54 patients (46%) receiving cisplatin alone and in 52 of 74 patients (70%) receiving cisplatin + 5-FU. The latter regimen was particularly associated with increased rates of mucositis (p = 0.027) and acute skin toxicity (p = 0.001). Seven of 54 (13%) and 20 of 74 patients (27%) received only one chemotherapy course due to treatment-related acute toxicity. Late toxicity in terms of xerostomia, neck fibrosis, skin toxicity, and lymphedema was not significantly different. The 2-year locoregional control rates were 67% after cisplatin alone and 52% after cisplatin + 5-FU (p = 0.35). The metastases-free survival rates were 79% and 69%, respectively (p = 0.65), and the overall survival rates 70% and 51%, respectively (p = 0.10). On multivariate analysis, outcome was significantly associated with performance status, T-category, N-category, hemoglobin level prior to radiotherapy, and radiotherapy break > 1 week. Conclusion: two courses of fractionated cisplatin (20 mg/m2/day) alone appear preferable, as this regimen resulted in similar outcome and late toxicity as two courses of cisplatin + 5-FU, but in significantly less acute toxicity. (orig.)

  12. Hypocholesterolaemic Activity of Lupin Peptides: Investigation on the Crosstalk between Human Enterocytes and Hepatocytes Using a Co-Culture System Including Caco-2 and HepG2 Cells.

    Science.gov (United States)

    Lammi, Carmen; Zanoni, Chiara; Ferruzza, Simonetta; Ranaldi, Giulia; Sambuy, Yula; Arnoldi, Anna

    2016-01-01

    Literature indicates that peptic and tryptic peptides derived from the enzymatic hydrolysis of lupin protein are able to modulate cholesterol metabolism in human hepatic HepG2 cells and that part of these peptides are absorbed in a small intestine model based on differentiated human Caco-2 cells. In this paper, a co-culture system, including Caco-2 and HepG2 cells, was investigated with two objectives: (a) to verify whether cholesterol metabolism in HepG2 cells was modified by the peptides absorption through Caco-2 cells; (b) to investigate how lupin peptides influence cholesterol metabolism in Caco-2 cells. The experiments showed that the absorbed peptides, not only maintained their bioactivity on HepG2 cells, but that this activity was improved by the crosstalk of the two cells systems in co-culture. In addition, lupin peptides showed a positive influence on cholesterol metabolism in Caco-2 cells, decreasing the proprotein convertase subtilisin/kexin type 9 (PCSK9) secretion. PMID:27455315

  13. Hypocholesterolaemic Activity of Lupin Peptides: Investigation on the Crosstalk between Human Enterocytes and Hepatocytes Using a Co-Culture System Including Caco-2 and HepG2 Cells

    Directory of Open Access Journals (Sweden)

    Carmen Lammi

    2016-07-01

    Full Text Available Literature indicates that peptic and tryptic peptides derived from the enzymatic hydrolysis of lupin protein are able to modulate cholesterol metabolism in human hepatic HepG2 cells and that part of these peptides are absorbed in a small intestine model based on differentiated human Caco-2 cells. In this paper, a co-culture system, including Caco-2 and HepG2 cells, was investigated with two objectives: (a to verify whether cholesterol metabolism in HepG2 cells was modified by the peptides absorption through Caco-2 cells; (b to investigate how lupin peptides influence cholesterol metabolism in Caco-2 cells. The experiments showed that the absorbed peptides, not only maintained their bioactivity on HepG2 cells, but that this activity was improved by the crosstalk of the two cells systems in co-culture. In addition, lupin peptides showed a positive influence on cholesterol metabolism in Caco-2 cells, decreasing the proprotein convertase subtilisin/kexin type 9 (PCSK9 secretion.

  14. Broken detailed balance at mesoscopic scales in active biological systems.

    Science.gov (United States)

    Battle, Christopher; Broedersz, Chase P; Fakhri, Nikta; Geyer, Veikko F; Howard, Jonathon; Schmidt, Christoph F; MacKintosh, Fred C

    2016-04-29

    Systems in thermodynamic equilibrium are not only characterized by time-independent macroscopic properties, but also satisfy the principle of detailed balance in the transitions between microscopic configurations. Living systems function out of equilibrium and are characterized by directed fluxes through chemical states, which violate detailed balance at the molecular scale. Here we introduce a method to probe for broken detailed balance and demonstrate how such nonequilibrium dynamics are manifest at the mesosopic scale. The periodic beating of an isolated flagellum from Chlamydomonas reinhardtii exhibits probability flux in the phase space of shapes. With a model, we show how the breaking of detailed balance can also be quantified in stationary, nonequilibrium stochastic systems in the absence of periodic motion. We further demonstrate such broken detailed balance in the nonperiodic fluctuations of primary cilia of epithelial cells. Our analysis provides a general tool to identify nonequilibrium dynamics in cells and tissues. PMID:27126047

  15. 3D nuclear organization of telomeres in the Hodgkin cell lines U-HO1 and U-HO1-PTPN1: PTPN1 expression prevents the formation of very short telomeres including "t-stumps"

    Directory of Open Access Journals (Sweden)

    Lemieux Bruno

    2010-12-01

    Full Text Available Abstract Background In cancer cells the three-dimensional (3D telomere organization of interphase nuclei into a telomeric disk is heavily distorted and aggregates are found. In Hodgkin's lymphoma quantitative FISH (3D Q-FISH reveals a major impact of nuclear telomere dynamics during the transition form mononuclear Hodgkin (H to diagnostic multinuclear Reed-Sternberg (RS cells. In vitro and in vivo formation of RS-cells is associated with the increase of very short telomeres including "t-stumps", telomere loss, telomeric aggregate formation and the generation of "ghost nuclei". Results Here we analyze the 3D telomere dynamics by Q-FISH in the novel Hodgkin cell line U-HO1 and its non-receptor protein-tyrosine phosphatase N1 (PTPN1 stable transfectant U-HO1-PTPN1, derived from a primary refractory Hodgkin's lymphoma. Both cell lines show equally high telomerase activity but U-HO1-PTPN differs from U-HO1 by a three times longer doubling time, low STAT5A expression, accumulation of RS-cells (p As expected, multinuclear U-HO1-RS-cells and multinuclear U-HO1-PTPN1-RS-cells differ from their mononuclear H-precursors by their nuclear volume (p Conclusion Abundant RS-cells without additional very short telomeres including "t-stumps", high rate of apoptosis, but low STAT5A expression, are hallmarks of the U-HO1-PTPN1 cell line. These characteristics are independent of telomerase activity. Thus, PTPN1 induced dephosphorylation of STAT5 with consecutive lack of Akt/PKB activation and cellular arrest in G2, promoting induction of apoptosis, appears as a possible pathogenetic mechanism deserving further experimental investigation.

  16. Optical modulator including grapene

    Energy Technology Data Exchange (ETDEWEB)

    Liu, Ming; Yin, Xiaobo; Zhang, Xiang

    2016-06-07

    The present invention provides for a one or more layer graphene optical modulator. In a first exemplary embodiment the optical modulator includes an optical waveguide, a nanoscale oxide spacer adjacent to a working region of the waveguide, and a monolayer graphene sheet adjacent to the spacer. In a second exemplary embodiment, the optical modulator includes at least one pair of active media, where the pair includes an oxide spacer, a first monolayer graphene sheet adjacent to a first side of the spacer, and a second monolayer graphene sheet adjacent to a second side of the spacer, and at least one optical waveguide adjacent to the pair.

  17. The dose dependent in vitro responses of MCF-7 and MDA-MB-231 cell lines to extracts of Vatica diospyroides symington type SS fruit include effects on mode of cell death

    OpenAIRE

    Theera Srisawat; Yaowapa Sukpondma; Potchanapond Graidist; Siriphon Chimplee; Kanyanatt Kanokwiroon

    2015-01-01

    Background: Vatica diospyroides type LS is a known source of valuable compounds for cancer treatment, however, in contrast little is known about therapeutic efficacy of type SS. Objective: This study focused on in vitro cytotoxicity of these fruit extracts, and the cell death mode they induce in breast cancer cells. Materials and Methods: Acetone extracts of fruit were tested for cytotoxicity against MCF-7 and MDA-MB-231 cell lines. The apoptosis and necrosis of these cells were quantified by...

  18. FOXC1 in human trabecular meshwork cells is involved in regulatory pathway that includes miR-204, MEIS2, and ITGβ1.

    Science.gov (United States)

    Paylakhi, Seyed Hassan; Moazzeni, Hamidreza; Yazdani, Shahin; Rassouli, Paniz; Arefian, Ehsan; Jaberi, Elham; Arash, Emad Heidari; Gilani, Ahmad Sadighi; Fan, Jian-Bing; April, Craig; Amin, Sadaf; Suri, Fatemeh; Elahi, Elahe

    2013-06-01

    Forkhead box C1 (FOXC1) is a transcription factor that affects eye development. FOXC1 is implicated in the etiology of glaucoma because mutations in the gene are among the causes of Axenfeld-Rieger syndrome which is often accompanied by glaucoma. Glaucoma is the second leading cause of blindness. It is a complex disorder whose genetic basis in most patients remains unknown. Microarrays expression analysis was performed to identify genes in human trabecular meshwork (TM) primary cultures that are affected by FOXC1 and genes that may have roles in glaucoma. This represents the first genome wide analysis of FOXC1 target genes in any tissue. FOXC1 knock down by siRNAs affected the expression of 849 genes. Results on selected genes were confirmed by real time PCR, immunoblotting, and dual luciferase reporter assays. Observation of MEIS2 as a FOXC1 target and consideration of FOXC1 as a potential target of miR-204 prompted testing the effect of this micro RNA on expression of FOXC1 and several genes identified by array analysis as FOXC1 target genes. It was observed that miR-204 caused decreased expression of FOXC1 and the FOXC1 target genes CLOCK, PLEKHG5, ITGβ1, and MEIS2 in the TM cultures. Expression of CLOCK, PLEKHG5, ITGβ1 has not previously been reported to be affected by miR-204. The data suggest existence of a complex regulatory pathway in the TM part of which includes interactions between FOXC1, miR-204, MEIS2, and ITGβ1. All these molecules are known to have TM relevant functions, and the TM is strongly implicated in the etiology of glaucoma. PMID:23541832

  19. Optomechanical details in injection-molded assemblies

    Science.gov (United States)

    Hebert, Raymond T.

    1995-12-01

    With the advent of low-cost electro-optic components such as LEDs, laser diodes and CCD imaging devices, the cost and performance demands now fall upon the optical subsystems in order to achieve realistic marketing targets for many emerging commercial and consumer products. One of the many benefits of injection-molded plastic optics is the diversity of features that are available to the design team. Once designed and incorporated into the tooling, many features are virtually free in high-volume production. These features can include mechanical details as well as optical functions. Registration features can be included for precisely positioning optical elements to one another or to other assemblies such as printed circuit boards or housings. Snaps, compression features, spring-loading elements, standoffs, self-tapping screws or ultrasonically weldable features can greatly facilitate ease of assembly.

  20. It's all in the details: methods in breast development and cancer

    OpenAIRE

    Bentires-Alj, Mohamed; Clarke, Robert B.; Jonkers, Jos; Smalley, Matthew; Stein, Torsten

    2009-01-01

    The inaugural European Network for Breast Development and Cancer (ENBDC) meeting on 'Methods in Mammary Gland Development and Cancer' was held in Weggis, Switzerland last April. The goal was to discuss the details of techniques used to study mammary gland biology and tumourigenesis. Highlights of this meeting included the use of four-colour fluorescence for protein co-localisation in tissue microarrays, genome analysis at single cell resolution, technical issues in the isolation of normal and...

  1. 4-IBP, a σ1 Receptor Agonist, Decreases the Migration of Human Cancer Cells, Including Glioblastoma Cells, In Vitro and Sensitizes Them In Vitro and In Vivo to Cytotoxic Insults of Proapoptotic and Proautophagic Drugs

    Directory of Open Access Journals (Sweden)

    Veronique Mégalizzi

    2007-05-01

    Full Text Available Although the molecular function of cr receptors has not been fully defined and the natural ligand(s is still not known, there is increasing evidence that these receptors and their ligands might play a significant role in cancer biology. 4-(N-tibenzylpiperidin-4-yl-4iodobenzamide (4-IBP, a selective σ1, agonist, has been used to investigate whether this compound is able to modify: 1 in vitro the migration and proliferation of human cancer cells; 2 in vitro the sensitivity of human glioblastoma cells to cytotoxic drugs; and 3 in vivo in orthotopic glioblastoma and non-small cell lung carcinoma (NSCLC models the survival of mice coadministered cytotoxic agents. 4-IBP has revealed weak anti proliferative effects on human U373-MG glioblastoma and C32 melanoma cells but induced marked concentration-dependent decreases in the growth of human A549 NSCLC and PC3 prostate cancer cells. The compound was also significantly antimigratory in all four cancer cell lines. This may result, at least in U373-MG cells, from modifications to the actin cytoskeleton. 4-IBP modified the sensitivity of U373-MG cells in vitro to proapoptotic lomustin and proautophagic temozolomide, and markedly decreased the expression of two proteins involved in drug resistance: glucosylceramide synthase and Rho guanine nucleotide dissociation inhibitor. In vivo, 4-IBP increased the antitumor effects of temozolomide and irinotecan in immunodeficient mice that were orthotopically grafted with invasive cancer cells.

  2. Analytic device including nanostructures

    KAUST Repository

    Di, Fabrizio, E.

    2015-07-02

    A device for detecting an analyte in a sample comprising: an array including a plurality of pixels, each pixel including a nanochain comprising: a first nanostructure, a second nanostructure, and a third nanostructure, wherein size of the first nanostructure is larger than that of the second nanostructure, and size of the second nanostructure is larger than that of the third nanostructure, and wherein the first nanostructure, the second nanostructure, and the third nanostructure are positioned on a substrate such that when the nanochain is excited by an energy, an optical field between the second nanostructure and the third nanostructure is stronger than an optical field between the first nanostructure and the second nanostructure, wherein the array is configured to receive a sample; and a detector arranged to collect spectral data from a plurality of pixels of the array.

  3. The dose dependent in vitro responses of MCF-7 and MDA-MB-231 cell lines to extracts of Vatica diospyroides symington type SS fruit include effects on mode of cell death

    Science.gov (United States)

    Srisawat, Theera; Sukpondma, Yaowapa; Graidist, Potchanapond; Chimplee, Siriphon; Kanokwiroon, Kanyanatt

    2015-01-01

    Background: Vatica diospyroides type LS is a known source of valuable compounds for cancer treatment, however, in contrast little is known about therapeutic efficacy of type SS. Objective: This study focused on in vitro cytotoxicity of these fruit extracts, and the cell death mode they induce in breast cancer cells. Materials and Methods: Acetone extracts of fruit were tested for cytotoxicity against MCF-7 and MDA-MB-231 cell lines. The apoptosis and necrosis of these cells were quantified by fluorescence activated cell sorter (FACS) and western blot analyses. Results: After 72 h of treatment, the 50% growth inhibition concentrations (IC50) levels were 16.21 ± 0.13 µg/mL against MCF-7 and 30.0 ± 4.30 µg/mL against MDA-MB-231, indicating high and moderate cytotoxicity, respectively. From the FACS results, we estimate that the cotyledon extract at half IC50 produced 11.7% dead MCF-7 cells via apoptosis, whereas another concentrations both apoptosis and necrosis modes co-existed in a dose-dependent manner. In MDA-MB-231 cell line, only the apoptosis was induced by the pericarp extract in a dose-dependent manner. With the extracts at half IC50 concentration, in both cells, the expression of p21 decreased while that of Bax increased within 12–48 h of dosing, confirming apoptosis induced by time-dependent responses. Apoptosis dependent on p53 was found in MCF-7, whereas the mutant p53 of MDA-MB-231 cells was expressed. Conclusion: The results indicate that fruit extracts of V. diospyroides have cytotoxic effects against MCF-7 and MDA-MB-231 cells via apoptosis pathway in a dose-dependent manner. This suggests that the extracts could provide active ingredients for the development, targeting breast cancer therapy. PMID:26109760

  4. Thirty Meter Telescope Detailed Science Case: 2015

    Science.gov (United States)

    Skidmore, Warren; TMT International Science Development Teams; Science Advisory Committee, TMT

    2015-12-01

    The TMT Detailed Science Case describes the transformational science that the Thirty Meter Telescope will enable. Planned to begin science operations in 2024, TMT will open up opportunities for revolutionary discoveries in essentially every field of astronomy, astrophysics and cosmology, seeing much fainter objects much more clearly than existing telescopes. Per this capability, TMT's science agenda fills all of space and time, from nearby comets and asteroids, to exoplanets, to the most distant galaxies, and all the way back to the very first sources of light in the universe. More than 150 astronomers from within the TMT partnership and beyond offered input in compiling the new 2015 Detailed Science Case. The contributing astronomers represent the entire TMT partnership, including the California Institute of Technology (Caltech), the Indian Institute of Astrophysics (IIA), the National Astronomical Observatories of the Chinese Academy of Sciences (NAOC), the National Astronomical Observatory of Japan (NAOJ), the University of California, the Association of Canadian Universities for Research in Astronomy (ACURA) and US associate partner, the Association of Universities for Research in Astronomy (AURA). Cover image: artist's rendition of the TMT International Observatory on Mauna Kea opening in the late evening before beginning operations.

  5. The Giant Cell.

    Science.gov (United States)

    Stockdale, Dennis

    1998-01-01

    Provides directions for the construction of giant plastic cells, including details for building and installing the organelles. Also contains instructions for preparing the ribosomes, nucleolus, nucleus, and mitochondria. (DDR)

  6. Detailed Chemical Kinetic Modeling of Cyclohexane Oxidation

    Energy Technology Data Exchange (ETDEWEB)

    Silke, E J; Pitz, W J; Westbrook, C K; Ribaucour, M

    2006-11-10

    A detailed chemical kinetic mechanism has been developed and used to study the oxidation of cyclohexane at both low and high temperatures. Reaction rate constant rules are developed for the low temperature combustion of cyclohexane. These rules can be used for in chemical kinetic mechanisms for other cycloalkanes. Since cyclohexane produces only one type of cyclohexyl radical, much of the low temperature chemistry of cyclohexane is described in terms of one potential energy diagram showing the reaction of cyclohexyl radical + O{sub 2} through five, six and seven membered ring transition states. The direct elimination of cyclohexene and HO{sub 2} from RO{sub 2} is included in the treatment using a modified rate constant of Cavallotti et al. Published and unpublished data from the Lille rapid compression machine, as well as jet-stirred reactor data are used to validate the mechanism. The effect of heat loss is included in the simulations, an improvement on previous studies on cyclohexane. Calculations indicated that the production of 1,2-epoxycyclohexane observed in the experiments can not be simulated based on the current understanding of low temperature chemistry. Possible 'alternative' H-atom isomerizations leading to different products from the parent O{sub 2}QOOH radical were included in the low temperature chemical kinetic mechanism and were found to play a significant role.

  7. Monte Carlo methods beyond detailed balance

    NARCIS (Netherlands)

    Schram, Raoul D.; Barkema, Gerard T.

    2015-01-01

    Monte Carlo algorithms are nearly always based on the concept of detailed balance and ergodicity. In this paper we focus on algorithms that do not satisfy detailed balance. We introduce a general method for designing non-detailed balance algorithms, starting from a conventional algorithm satisfying

  8. Identification of a Developmental Gene Expression Signature, Including HOX Genes, for the Normal Human Colonic Crypt Stem Cell Niche: Overexpression of the Signature Parallels Stem Cell Overpopulation During Colon Tumorigenesis

    OpenAIRE

    Bhatlekar, Seema; Addya, Sankar; Salunek, Moreh; Orr, Christopher R.; Surrey, Saul; McKenzie, Steven; Fields, Jeremy Z.; Boman, Bruce M

    2013-01-01

    Our goal was to identify a unique gene expression signature for human colonic stem cells (SCs). Accordingly, we determined the gene expression pattern for a known SC-enriched region—the crypt bottom. Colonic crypts and isolated crypt subsections (top, middle, and bottom) were purified from fresh, normal, human, surgical specimens. We then used an innovative strategy that used two-color microarrays (∼18,500 genes) to compare gene expression in the crypt bottom with expression in the other cryp...

  9. Detailed computation of hot-plasma atomic spectra

    OpenAIRE

    Pain, Jean-Christophe; Gilleron, Franck; Blenski, Thomas

    2015-01-01

    We present recent evolutions of the detailed opacity code SCO-RCG which combines statistical modelings of levels and lines with fine-structure calculations. The code now includes the Partially-Resolved-Transition-Array model, which allows one to replace a complex transition array by a small-scale detailed calculation preserving energy and variance of the genuine transition array and yielding improved high-order moments. An approximate method for studying the impact of strong magnetic field on...

  10. Displaying of Details in Subvoxel Accuracy

    Institute of Scientific and Technical Information of China (English)

    蔡文立; 陈天洲; 等

    1996-01-01

    Under the volume segmentation in voxel space,a lot of details,some fine and thin objects,are ignored.In order to accurately display these details,this paper has developed a methodology for volume segmentation in subvoxel space.In the subvoxel space,most of the “bridges”between adjacent layers are broken down.Based on the subvoxel space,an automatic segmentation algorithm reserving details is discussed.After segmentation,volume data in subvoxel space are reduced to original voxel space.Thus,the details with width of only one or several voxels are extracted and displayed.

  11. Numerical simulation of spark ignition including ionization

    NARCIS (Netherlands)

    Thiele, M; Selle, S; Riedel, U; Warnatz, J; Maas, U

    2000-01-01

    A detailed understanding of the processes associated Midi spark ignition, as a first step during combustion, is of great importance fur clean operation of spark ignition engines. In the past 10 years. a growing concern for environmental protection, including low emission of pollutants, has increased

  12. Halo-substituted thiosemicarbazones and their copper(II), nickel(II) complexes: Detailed spectroscopic characterization and study of antitumour activity against HepG2 human hepatoblastoma cells

    Science.gov (United States)

    Jagadeesh, M.; Kalangi, Suresh K.; Sivarama Krishna, L.; Reddy, A. Varada

    2014-01-01

    Copper(II) and nickel(II) complexes of two different halogen substituted thiosemicarbazone ligands were synthesized. The ligands 3,4-difluoroacetophenone thiosemicarbazone (1) and 2-bromo-4'-chloroacetophenone thiosemicarbazone (2) were characterized and confirmed spectroscopically by FT-IR, FT-Raman, UV-vis and fluorescence spectral analysis, while the respective copper(II) complexes [Cu(C9H9N3F2S)2Cl2] (1a), [Cu(C9H9N3ClBrS)2Cl2] (2a) and nickel(II) complexes [Ni(C9H9N3F2S)2] (1b), [Ni(C9H9N3ClBrS)2] (2b) were characterized by FT-IR, UV-vis and electron paramagnetic spectroscopy (EPR). The EPR spectra of the Cu(II) complexes provided the rhombic octahedral and axial symmetry of the complexes 1a and 2a respectively. For the complex 1a, the g values calculated as g1 = 2.1228, g2 = 2.0706 and g3 = 2.001 between 2900 and 3300 G. While for the complex 2a, a set of two resonance absorptions were observed. The synthesized compounds were tested for antitumor activity and showed that the ability to kill liver cancer cells significantly. Out of all the synthesized compounds, copper(II) complexes 1a and 2a showed high cytotoxic effect on liver cancer cells with 67.51% and 42.77% of cytotoxicity respectively at 100 μM.

  13. Detailed Aerosol Characterization using Polarimetric Measurements

    Science.gov (United States)

    Hasekamp, Otto; di Noia, Antonio; Stap, Arjen; Rietjens, Jeroen; Smit, Martijn; van Harten, Gerard; Snik, Frans

    2016-04-01

    Anthropogenic aerosols are believed to cause the second most important anthropogenic forcing of climate change after greenhouse gases. In contrast to the climate effect of greenhouse gases, which is understood relatively well, the negative forcing (cooling effect) caused by aerosols represents the largest reported uncertainty in the most recent assessment of the International Panel on Climate Change (IPCC). To reduce the large uncertainty on the aerosol effects on cloud formation and climate, accurate satellite measurements of aerosol optical properties (optical thickness, single scattering albedo, phase function) and microphysical properties (size distribution, refractive index, shape) are essential. There is growing consensus in the aerosol remote sensing community that multi-angle measurements of intensity and polarization are essential to unambiguously determine all relevant aerosol properties. This presentations adresses the different aspects of polarimetric remote sensing of atmospheric aerosols, including retrieval algorithm development, validation, and data needs for climate and air quality applications. During past years, at SRON-Netherlands Instite for Space Research retrieval algorithms have been developed that make full use of the capabilities of polarimetric measurements. We will show results of detailed aerosol properties from ground-based- (groundSPEX), airborne- (NASA Research Scanning Polarimeter), and satellite (POLDER) measurements. Also we will discuss observational needs for future instrumentation in order to improve our understanding of the role of aerosols in climate change and air quality.

  14. Detailed Analysis of Motor Unit Activity

    DEFF Research Database (Denmark)

    Nikolic, Mile; Sørensen, John Aasted; Dahl, Kristian;

    1997-01-01

    System for decomposition of EMG signals intotheir constituent motor unit potentials and their firing patterns.The aim of the system is detailed analysis ofmotor unit variability.......System for decomposition of EMG signals intotheir constituent motor unit potentials and their firing patterns.The aim of the system is detailed analysis ofmotor unit variability....

  15. Understanding brains: details, intuition, and big data.

    Directory of Open Access Journals (Sweden)

    Eve Marder

    2015-05-01

    Full Text Available Understanding how the brain works requires a delicate balance between the appreciation of the importance of a multitude of biological details and the ability to see beyond those details to general principles. As technological innovations vastly increase the amount of data we collect, the importance of intuition into how to analyze and treat these data may, paradoxically, become more important.

  16. UNIVERSALIZATION OF BUS BODY FACING DETAILS AND PRODUCTION TOOLING FOR THEIR MANUFACTURING

    Directory of Open Access Journals (Sweden)

    M. Taranenko

    2009-01-01

    Full Text Available The directions concerning bus body production costs reduction based on large-size facing details stamping on electric-hydraulic presses are considered. The engineering of unified large-size facing detail blanks, manufacturing processes and variants of unified technological tooling for bus body facing details production, including simple- or multi-type details are described.

  17. SEPAC flight software detailed design specifications, volume 1

    Science.gov (United States)

    1982-01-01

    The detailed design specifications (as built) for the SEPAC Flight Software are defined. The design includes a description of the total software system and of each individual module within the system. The design specifications describe the decomposition of the software system into its major components. The system structure is expressed in the following forms: the control-flow hierarchy of the system, the data-flow structure of the system, the task hierarchy, the memory structure, and the software to hardware configuration mapping. The component design description includes details on the following elements: register conventions, module (subroutines) invocaton, module functions, interrupt servicing, data definitions, and database structure.

  18. Analysis of Common Fatigue Details in Steel Truss Structures

    Institute of Scientific and Technical Information of China (English)

    张玉玲; 潘际炎; 潘际銮

    2004-01-01

    Generally, the number of fatigue cycles, the range of the repeated stresses, and the type of the structural details are the key factors affecting fatigue in large-scale welded structures. Seven types of structure details were tested using a 2000-kN hydraulic-pressure-servo fatigue machine to imitate fatigue behavior in modern steel-truss-structures fabricated using thicker welded steel plates and integral joint technology. The details included longitudinal edge welds, welded attachment affecting detail, integral joint, and weld repairs on plate edges. The fatigue damage locations show that the stress (normal or shear), the shape, and the location of the weld start and end points are three major factors reducing the fatigue strength. The test results can be used for similar large structures.

  19. Development of a Modular Assay for Detailed Immunophenotyping of Peripheral Human Whole Blood Samples by Multicolor Flow Cytometry

    Directory of Open Access Journals (Sweden)

    Paul F. Rühle

    2016-08-01

    Full Text Available The monitoring of immune cells gained great significance in prognosis and prediction of therapy responses. For analyzing blood samples, the multicolor flow cytometry has become the method of choice as it combines high specificity on single cell level with multiple parameters and high throughput. Here, we present a modular assay for the detailed immunophenotyping of blood (DIoB that was optimized for an easy and direct application in whole blood samples. The DIoB assay characterizes 34 immune cell subsets that circulate the peripheral blood including all major immune cells such as T cells, B cells, natural killer (NK cells, monocytes, dendritic cells (DCs, neutrophils, eosinophils, and basophils. In addition, it evaluates their functional state and a few non-leukocytes that also have been associated with the outcome of cancer therapy. This DIoB assay allows a longitudinal and close-meshed monitoring of a detailed immune status in patients requiring only 2.0 mL of peripheral blood and it is not restricted to peripheral blood mononuclear cells. It is currently applied for the immune monitoring of patients with glioblastoma multiforme (IMMO-GLIO-01 trial, NCT02022384, pancreatic cancer (CONKO-007 trial, NCT01827553, and head and neck cancer (DIREKHT trial, NCT02528955 and might pave the way for immune biomarker identification for prediction and prognosis of therapy outcome.

  20. Detailed Globes Enhance Education and Recreation

    Science.gov (United States)

    2007-01-01

    Orbis World Globes creates inflatable globes-Earthballs-in many sizes that depict Earth as it is seen from space, complete with atmospheric cloud cover. Orbis designs and produces the most visually authentic replicas of Earth ever created, and NASA took notice of Orbis globes and employed a 16-inch diameter EarthBall for an educational film it made aboard the STS-45 shuttle mission. Orbis later collaborated with NASA to create two 16-foot diameter world globes for display at the 2002 Olympic Winter Games in Salt Lake City, using more detailed satellite imagery. The satellite image now printed on all Orbis globes displays 1-kilometer resolution and is 21,600 by 43,200 pixels in size, and Orbis globes are otherwise meteorologically accurate, though the cloud cover has been slightly reduced in order for most of the landforms to be visible. Orbis also developed the exclusive NightGlow Cities feature, enabling EarthBalls to display the world's cities as they appear as the Earth revolves from daylight into night. Orbis inflatable globes are available in sizes from 1 to 100 feet in diameter, with the most common being the standard 16-inch and 1-meter diameter EarthBalls. Applications include educational uses from preschools to universities, games, and for a variety of display purposes at conferences, trade shows, festivals, concerts, and parades. A 16-foot diameter Orbis globe was exhibited at the United Nations' World Urban Forum, in Vancouver, Canada; the Space 2006 conference, in San Jose, California; and the X-Prize Cup Personal Spaceflight Exposition in Las Cruces, New Mexico.

  1. Post Entitlement Management Information - Detail Database

    Data.gov (United States)

    Social Security Administration — Contains data that supports the detailed and aggregate receipt, pending and clearance data, as well as other strategic and tactical MI for many Title II and Title...

  2. Fine phonetic detail and intonational meaning

    OpenAIRE

    Post, B.M.B.; Imperio, M. d'; Gussenhoven, C.

    2007-01-01

    International audience The development of theories about form-function relations in intonation should be informed by a better understanding of the dependencies that hold among different phonetic parameters. Fine phonetic detail encodes both linguistically structured meaning and paralinguistic meaning.

  3. Template Assembly for Detailed Urban Reconstruction

    KAUST Repository

    Nan, Liangliang

    2015-05-04

    We propose a new framework to reconstruct building details by automatically assembling 3D templates on coarse textured building models. In a preprocessing step, we generate an initial coarse model to approximate a point cloud computed using Structure from Motion and Multi View Stereo, and we model a set of 3D templates of facade details. Next, we optimize the initial coarse model to enforce consistency between geometry and appearance (texture images). Then, building details are reconstructed by assembling templates on the textured faces of the coarse model. The 3D templates are automatically chosen and located by our optimization-based template assembly algorithm that balances image matching and structural regularity. In the results, we demonstrate how our framework can enrich the details of coarse models using various data sets.

  4. Bacteria repelling poly(methylmethacrylate-co-dimethylacrylamide) coatings for biomedical devices† †Electronic supplementary information (ESI) available: Polymer microarray screening, including analysis of bacterial adhesion by fluorescence microscopy and SEM, and chemical composition of bacteria repelling polymers identified in the screen; polymer synthesis and characterisation; preparation of catheter pieces and solvent studies, and details for confocal imaging/analysis. See DOI: 10.1039/c4tb01129e Click here for additional data file.

    Science.gov (United States)

    Venkateswaran, Seshasailam; Wu, Mei; Gwynne, Peter J.; Hardman, Ailsa; Lilienkampf, Annamaria; Pernagallo, Salvatore; Blakely, Garry; Swann, David G.

    2014-01-01

    Nosocomial infections due to bacteria have serious implications on the health and recovery of patients in a variety of medical scenarios. Since bacterial contamination on medical devices contributes to the majority of nosocomical infections, there is a need for redesigning the surfaces of medical devices, such as catheters and tracheal tubes, to resist the binding of bacteria. In this work, polyurethanes and polyacrylates/acrylamides, which resist binding by the major bacterial pathogens underpinning implant-associated infections, were identified using high-throughput polymer microarrays. Subsequently, two ‘hit’ polymers, PA13 (poly(methylmethacrylate-co-dimethylacrylamide)) and PA515 (poly(methoxyethylmethacrylate-co-diethylaminoethylacrylate-co-methylmethacrylate)), were used to coat catheters and substantially shown to decrease binding of a variety of bacteria (including isolates from infected endotracheal tubes and heart valves from intensive care unit patients). Catheters coated with polymer PA13 showed up to 96% reduction in bacteria binding in comparison to uncoated catheters. PMID:25580245

  5. Details of the battle to control Campeche Bay spill

    Energy Technology Data Exchange (ETDEWEB)

    1979-11-01

    Details of the battle to control Campeche Bay spill from Petroleos Mexicanos' well at Ixtoc 1 are given, including the poor performance of ''Operation Sombrero'' and air and surface monitoring of spill transport, particularly by the US Coast Guard.

  6. Eros details enhanced by computer processing

    Science.gov (United States)

    2000-01-01

    The NEAR camera's ability to show details of Eros's surface is limited by the spacecraft's distance from the asteroid. That is, the closer the spacecraft is to the surface, the more that details are visible. However mission scientists regularly use computer processing to squeeze an extra measure of information from returned data. In a technique known as 'superresolution', many images of the same scene acquired at very, very slightly different camera pointing are carefully overlain and processed to bright out details even smaller than would normally be visible. In this rendition constructed out of 20 image frames acquired Feb. 12, 2000, the images have first been enhanced ('high-pass filtered') to accentuate small-scale details. Superresolution was then used to bring out features below the normal ability of the camera to resolve.Built and managed by The Johns Hopkins University Applied Physics Laboratory, Laurel, Maryland, NEAR was the first spacecraft launched in NASA's Discovery Program of low-cost, small-scale planetary missions. See the NEAR web page at http://near.jhuapl.edu for more details.

  7. Contribution to a Theory of Detailed Design

    DEFF Research Database (Denmark)

    Mortensen, Niels Henrik

    1999-01-01

    It has been recognised, that literature actually do not propose a theory of detailed design. In this paper a theory contribution is proposed, linking part design to organ design and allowing a type of functional reasoning. The proposed theory satisfies our need for explaining the nature of a part...... structure, for support of synthesis of part structure, i.e. detailed design, and our need for digital modelling of part structures.The aim of this paper is to contribute to a design theory valid for detailed design. The proposal is based upon the theory's ability to explain the nature of machine parts...... and assemblies, to support the synthesis of parts and to allow the modelling, especially digital modelling of a part structure. The contribution is based upon Theory of Technical Systems, Hubka, and the Domain Theory, Andreasen. This paper is based on a paper presented at ICED 99, Mortensen, but focus...

  8. Local address and emergency contact details

    CERN Multimedia

    2013-01-01

    The HR Department would like to remind members of the personnel that they are responsible for ensuring that their personal data concerning local address and preferred emergency contact details remains valid and up-to-date.   Both are easily accessible via the links below: Local address: https://edh.cern.ch/Document/Personnel/LocalAddressChange   Emergency contacts: https://edh.cern.ch/Document/Personnel/EC   Please take a few minutes to check your details and modify if necessary. Thank you in advance. HR Department Head Office

  9. Detail in architecture: Between arts & crafts

    Science.gov (United States)

    Dulencin, Juraj

    2016-06-01

    Architectural detail represents an important part of architecture. Not only can it be used as an identifier of a specific building but at the same time enhances the experience of the realized project. Within it lie the signs of a great architect and clues to understanding his or her way of thinking. It is therefore the central topic of a seminar offered to architecture students at the Brno University of Technology. During the course of the semester-long class the students acquaint themselves with atypical architectural details of domestic and international architects by learning to read them, understand them and subsequently draw them by creating architectural blueprints. In other words, by general analysis of a detail the students learn theoretical thinking of its architect who, depending on the nature of the design, had to incorporate a variety of techniques and crafts. Students apply this analytical part to their own architectural detail design. The methodology of the seminar consists of experiential learning by project management and is complemented by a series of lectures discussing a diversity of details as well as materials and technologies required to implement it. The architectural detail design is also part of students' bachelors thesis, therefore, the realistic nature of their blueprints can be verified in the production process of its physical counterpart. Based on their own documentation the students choose the most suitable manufacturing process whether it is supplied by a specific technology or a craftsman. Students actively participate in the production and correct their design proposals in real scale with the actual material. A student, as a future architect, stands somewhere between a client and an artisan, materializes his or her idea and adjusts the manufacturing process so that the final detail fulfills aesthetic consistency and is in harmony with its initial concept. One of the very important aspects of the design is its economic cost, an

  10. Immunofluorescent Staining of Mouse Intestinal Stem Cells

    Science.gov (United States)

    O’Rourke, Kevin P.; Dow, Lukas E; Lowe, Scott W

    2016-01-01

    Immunofluorescent staining of organoids can be performed to visualize molecular markers of cell behavior. For example, cell proliferation marked by incorporation of nucleotide (EdU), or to observe markers of intestinal differentiation including paneth cells, goblet cells, or enterocytes (see Figure 1). In this protocol we detail a method to fix, permeabilize, stain and mount intestinal organoids for analysis by immunofluorescent confocal microscopy.

  11. Surface Detail Capturing for Realistic Facial Animation

    Institute of Scientific and Technical Information of China (English)

    Pei-Hsuan Tu; I-Chen Lin; Jeng-Sheng Yeh; Rung-Huei Liang; Ming Ouhyoung

    2004-01-01

    In this paper, a facial animation system is proposed for capturing both geometrical information and illumination changes of surface details, called expression details, from video clips simultaneously, and the captured data can be widely applied to different 2D face images and 3D face models. While tracking the geometric data,we record the expression details by ratio images. For 2D facial animation synthesis, these ratio images are used to generate dynamic textures. Because a ratio image is obtained via dividing colors of an expressive face by those of a neutral face, pixels with ratio value smaller than one are where a wrinkle or crease appears. Therefore, the gradients of the ratio value at each pixel in ratio images are regarded as changes of a face surface, and original normals on the surface can be adjusted according to these gradients. Based on this idea, we can convert the ratio images into a sequence of normal maps and then apply them to animated 3D model rendering. With the expression detail mapping, the resulted facial animations are more life-like and more expressive.

  12. New details emerge from the Einstein files

    CERN Multimedia

    Overbye, D

    2002-01-01

    For many years the FBI spied on Einstein. New details of this surveilance are emerging in "The Einstein File: J. Edgar Hoover's Secret War Against the World's Most Famous Scientist," by Fred Jerome, who sued the government with the help of the Public Citizen Litigation Group to obtain a less censored version of the file (1 page).

  13. Big Heads, Small Details and Autism

    Science.gov (United States)

    White, Sarah; O'Reilly, Helen; Frith, Uta

    2009-01-01

    Autism is thought to be associated with a bias towards detail-focussed processing. While the cognitive basis remains controversial, one strong hypothesis is that there are high processing costs associated with changing from local into global processing. A possible neural mechanism underlying this processing style is abnormal neural connectivity;…

  14. Analysis of local influences in structural details of the bridges

    Directory of Open Access Journals (Sweden)

    Adam RUDZIK

    2015-03-01

    Full Text Available The article analyses the problems of local influences in structural details of bridges as the critical locations, whose damages or excessive force may directly affect the safety of users. These analyses are shown on selected examples. Presented is the example of local changes in the forms of proper vibrations in the node of the truss bridge that can be used in expert issues concerning the causes of damages. The second example are the changes in stresses in the stay cable anchorage element including the nonlinear material models. Models of this type can be successfully used by engineers as they allow for analysis of selected structural details without the need for detailed mapping of the entire structure, but only a selected section.

  15. Crystal structure of listeriolysin O reveals molecular details of oligomerization and pore formation

    Science.gov (United States)

    Köster, Stefan; van Pee, Katharina; Hudel, Martina; Leustik, Martin; Rhinow, Daniel; Kühlbrandt, Werner; Chakraborty, Trinad; Yildiz, Özkan

    2014-04-01

    Listeriolysin O (LLO) is an essential virulence factor of Listeria monocytogenes that causes listeriosis. Listeria monocytogenes owes its ability to live within cells to the pH- and temperature-dependent pore-forming activity of LLO, which is unique among cholesterol-dependent cytolysins. LLO enables the bacteria to cross the phagosomal membrane and is also involved in activation of cellular processes, including the modulation of gene expression or intracellular Ca2+ oscillations. Neither the pore-forming mechanism nor the mechanisms triggering the signalling processes in the host cell are known in detail. Here, we report the crystal structure of LLO, in which we identified regions important for oligomerization and pore formation. Mutants were characterized by determining their haemolytic and Ca2+ uptake activity. We analysed the pore formation of LLO and its variants on erythrocyte ghosts by electron microscopy and show that pore formation requires precise interface interactions during toxin oligomerization on the membrane.

  16. Detailed sectional anatomy of the spine

    International Nuclear Information System (INIS)

    Morphologic studies on the human spine constitute a special challenge because of the spine's complex topographic anatomy and the intimate relationship between the supporting skeleton and the contiguous soft tissues (muscles, discs, joint capsules) as well as the neurovascular contents of the spinal canal and intervertebral foramina. The improving resolution and multiplanar image reformatting capabilities of modern CT scanners call for accurate anatomic reference material. Such anatomic images should be available without distortion, in natural colors, and in considerable detail. The images should present the anatomy in the correct axial, sagittal, and coronal planes and should also be sufficiently closely spaced so as to follow the thin cuts of modern CT scanners. This chapter details one of several recent attempts to correlate gross anatomy with the images depicted by high-resolution CT. The methods of specimen preparation, sectioning, and photographing have been documented elsewhere

  17. Detailed Jet Dynamics in a Collapsing Bubble

    Science.gov (United States)

    Supponen, Outi; Obreschkow, Danail; Kobel, Philippe; Farhat, Mohamed

    2015-12-01

    We present detailed visualizations of the micro-jet forming inside an aspherically collapsing cavitation bubble near a free surface. The high-quality visualizations of large and strongly deformed bubbles disclose so far unseen features of the dynamics inside the bubble, such as a mushroom-like flattened jet-tip, crown formation and micro-droplets. We also find that jetting near a free surface reduces the collapse time relative to the Rayleigh time.

  18. Thirty Meter Telescope Detailed Science Case: 2015

    OpenAIRE

    Skidmore, Warren; Dell'Antonio, Ian; Fukugawa, Misato; Goswami, Aruna; Hao, Lei; Jewitt, David; Laughlin, Greg; Steidel, Charles; Hickson, Paul; Simard, Luc; Schöck, Matthias; Treu, Tommaso; Cohen, Judith; Anupama, G. C; Dickinson, Mark

    2015-01-01

    The TMT Detailed Science Case describes the transformational science that the Thirty Meter Telescope will enable. Planned to begin science operations in 2024, TMT will open up opportunities for revolutionary discoveries in essentially every field of astronomy, astrophysics and cosmology, seeing much fainter objects much more clearly than existing telescopes. Per this capability, TMT's science agenda fills all of space and time, from nearby comets and asteroids, to exoplanets, to the most dist...

  19. The OPERA neutrino velocity measurement: Analysis details

    International Nuclear Information System (INIS)

    The OPERA neutrino experiment at the underground Gran Sasso Laboratory has measured the velocity of neutrinos from the CERN CNGS beam over a baseline of about 730 km. An early arrival time of CNGS muon neutrinos with respect to the one computed assuming the speed of light in vacuum was measured. Details on the statistical analysis of the nominal beam data sample and the short-bunch analysis as well as additional cross checks and an outlook of upcoming measurements are discussed.

  20. Detailed spectral analysis of decellularized skin implants

    Science.gov (United States)

    Timchenko, E. V.; Timchenko, P. E.; Volova, L. T.; Dolgushkin, D. A.; Shalkovsky, P. Y.; Pershutkina, S. V.

    2016-08-01

    The resutls of detailed analysis of donor skin implants using Raman spectroscopy method are presented. Fourier-deconvolution method was used to separate overlapping spectrum lines and to improve its informativeness. Based on the processed spectra were introduced coefficients that represent changes in relative concentration of implant components, which determines the quality of implants. It was established that Raman spectroscopy method can be used in assessment of skin implants.

  1. Structural concepts and details for seismic design

    Energy Technology Data Exchange (ETDEWEB)

    1991-09-01

    This manual discusses building and building component behavior during earthquakes, and provides suggested details for seismic resistance which have shown by experience to provide adequate performance during earthquakes. Special design and construction practices are also described which, although they might be common in some high-seismic regions, may not be common in low and moderate seismic-hazard regions of the United States. Special attention is given to describing the level of detailing appropriate for each seismic region. The UBC seismic criteria for all seismic zones is carefully examined, and many examples of connection details are given. The general scope of discussion is limited to materials and construction types common to Department of Energy (DOE) sites. Although the manual is primarily written for professional engineers engaged in performing seismic-resistant design for DOE facilities, the first two chapters, plus the introductory sections of succeeding chapters, contain descriptions which are also directed toward project engineers who authorize, review, or supervise the design and construction of DOE facilities. 88 refs., 188 figs.

  2. Long-term CD4+ T-cell count evolution after switching from regimens including HIV nucleoside reverse transcriptase inhibitors (NRTI plus protease inhibitors to regimens containing NRTI plus non-NRTI or only NRTI

    Directory of Open Access Journals (Sweden)

    Cicconi Paola

    2011-01-01

    Full Text Available Abstract Background Data regarding CD4+ recovery after switching from protease inhibitor (PI-based regimens to regimens not containing PI are scarce. Methods Subjects with virological success on first-PI-regimens who switched to NNRTI therapy (NNRTI group or to nucleoside reverse transcriptase (NRTI-only (NRTI group were studied. The effect of the switch on the ongoing CD4+ trend was assessed by two-phase linear regression (TPLR, allowing us to evaluate whether a change in the CD4+ trend (hinge occurred and the time of its occurrence. Furthermore, we described the evolution of the frequencies in CD4-count classes across four relevant time-points (baseline, before and immediately after the switch, and last visit. Finally, we explored whether the CD4+ counts evolved differently in patients who switched to NNRTI or NRTI-only regimens by considering: the overall CD4+ trends, the time to CD4+≥ 500/mm3 after the switch, and the area-under-the-curve (AUC of the CD4+ after the switch. Results Eight hundred and ninety-six patients, followed for a median of 2,121 days, were included. At TPLR, hinges occurred in 581/844 (68.9%, but in only 40/581 (6.9% within a time interval (180 days compatible with a possible relationship to the switch; furthermore, in 19/40 cases, CD4+ counts appeared to decrease after the hinges. In comparison with the NNRTI group, the NRTI group showed CD4+ count greater at baseline (P = 0.0234 and before the switch (P ≤ 0.0001, superior CD4+ T-cell increases after HAART was started, lower probability of not achieving CD4+ ≥ 500/mm3 (P = 0.0024, and, finally, no significant differences in the CD4+ T-cell AUC after the switch after adjusting for possible confounders (propensity score and pre-switch AUC. Persistence at CD4+ 3 was observed in 34/435 (7.5% patients, and a decrease below this level was found in only 10/259 (3.9% with baseline CD4+ ≥ 350/mm3. Conclusions Switching from first-line PI to NNRTI- or NRTI-based regimens

  3. Detailed Analysis of Electrical Characteristics of an InGaP/lnGaAs/Ge Triple-junction Solar Cell%三结砷化镓光伏电池电学特性的理论和实验分析

    Institute of Scientific and Technical Information of China (English)

    王子龙; 张华; 刘业风; 吴银龙; 颜慧磊; 张聪

    2013-01-01

    太阳能高倍聚光光伏发电系统(high concentration photovoltaic system,HCPV)将高效聚光光伏电池与聚光器利用组合在一起,具有较高的效率。针对三结聚光 InGaP/lnGaAs/Ge 叠层光伏电池具有较高效率且温度特性较好的特点,基于单二极管模型等效电路,建立了三结聚光InGaP/lnGaAs/Ge叠层光伏电池电学特性的数学模型,并在不同聚光倍数下(120、130、140和150倍),对三结砷化镓光伏电池的开路电压和电池效率进行了理论分析和计算,并与实验结果进行了对比。结果表明:三结砷化镓光伏电池开路电压和电池效率随着聚光比的逐步增大而增加,随着电池温度的增大而减小。实验结果与理论计算进行了对比,在相同电池温度下,开路电压和电池效率的误差分别为2.04%和8.4%。所建立的三结砷化镓光伏电池的数学模型,为研究其电学性能提供了一定的理论基础,可用来分析和讨论相关参数对高倍聚光式光伏系统性能的影响。%High concentration photovoltaic system combined high efficient triple-junction solar cell with concentrator, which had higher conversion efficiency compared with traditional silicon solar cell. The mathematical model of electrical characteristic for triple-junction solar cell which had excellent performance both in efficiency and temperature characteristic was established based on the one-diode equivalent circuit cell model. Detailed analysis on the influence factors of open-circuit voltage and cell efficiency for triple-junction solar cell output electrical characteristic under high concentration (120X、130X、140X and 150X) had been done, and the calculated results was compared with experimental date. The results show that under high concentrated light intensity, the open-circuit voltage and cell efficiency of the triple-junction solar cell were increasing as the concentration ratio increased. What opposite with it

  4. A time dependent anatomically detailed model of cardiac conduction

    Science.gov (United States)

    Saxberg, B. E.; Grumbach, M. P.; Cohen, R. J.

    1985-01-01

    In order to understand the determinants of transitions in cardiac electrical activity from normal patterns to dysrhythmias such as ventricular fibrillation, we are constructing an anatomically and physiologically detailed finite element simulation of myocardial electrical propagation. A healthy human heart embedded in paraffin was sectioned to provide a detailed anatomical substrate for model calculations. The simulation of propagation includes anisotropy in conduction velocity due to fiber orientation as well as gradients in conduction velocities, absolute and relative refractory periods, action potential duration and electrotonic influence of nearest neighbors. The model also includes changes in the behaviour of myocardial tissue as a function of the past local activity. With this model, we can examine the significance of fiber orientation and time dependence of local propagation parameters on dysrhythmogenesis.

  5. Examination of the regulatory frameworks applicable to biologic drugs (including stem cells and their progeny) in Europe, the U.S., and Australia: part I--a method of manual documentary analysis.

    Science.gov (United States)

    Ilic, Nina; Savic, Snezana; Siegel, Evan; Atkinson, Kerry; Tasic, Ljiljana

    2012-12-01

    Recent development of a wide range of regulatory standards applicable to production and use of tissues, cells, and other biologics (or biologicals), as advanced therapies, indicates considerable interest in the regulation of these products. The objective of this study was to analyze and compare high-tier documents within the Australian, European, and U.S. biologic drug regulatory environments using qualitative methodology. Cohort 1 of the selected 18 high-tier regulatory documents from the European Medicines Agency (EMA), the U.S. Food and Drug Administration (FDA), and the Therapeutic Goods Administration (TGA) regulatory frameworks were subject to a manual documentary analysis. These documents were consistent with the legal requirements for manufacturing and use of biologic drugs in humans and fall into six different categories. Manual analysis included a terminology search. The occurrence, frequency, and interchangeable use of different terms and phrases were recorded in the manual documentary analysis. Despite obvious differences, manual documentary analysis revealed certain consistency in use of terminology across analyzed frameworks. Phrase search frequencies have shown less uniformity than the search of terms. Overall, the EMA framework's documents referred to "medicinal products" and "marketing authorization(s)," the FDA documents discussed "drug(s)" or "biologic(s)," and the TGA documents referred to "biological(s)." Although high-tier documents often use different terminology they share concepts and themes. Documents originating from the same source have more conjunction in their terminology although they belong to different frameworks (i.e., Good Clinical Practice requirements based on the Declaration of Helsinki, 1964). Automated (software-based) documentary analysis should be obtained for the conceptual and relational analysis.

  6. Detailed modeling of mountain wave PSCs

    Directory of Open Access Journals (Sweden)

    S. Fueglistaler

    2003-01-01

    Full Text Available Polar stratospheric clouds (PSCs play a key role in polar ozone depletion. In the Arctic, PSCs can occur on the mesoscale due to orographically induced gravity waves. Here we present a detailed study of a mountain wave PSC event on 25-27 January 2000 over Scandinavia. The mountain wave PSCs were intensively observed by in-situ and remote-sensing techniques during the second phase of the SOLVE/THESEO-2000 Arctic campaign. We use these excellent data of PSC observations on 3 successive days to analyze the PSCs and to perform a detailed comparison with modeled clouds. We simulated the 3-dimensional PSC structure on all 3 days with a mesoscale numerical weather prediction (NWP model and a microphysical box model (using best available nucleation rates for ice and nitric acid trihydrate particles. We show that the combined mesoscale/microphysical model is capable of reproducing the PSC measurements within the uncertainty of data interpretation with respect to spatial dimensions, temporal development and microphysical properties, without manipulating temperatures or using other tuning parameters. In contrast, microphysical modeling based upon coarser scale global NWP data, e.g. current ECMWF analysis data, cannot reproduce observations, in particular the occurrence of ice and nitric acid trihydrate clouds. Combined mesoscale/microphysical modeling may be used for detailed a posteriori PSC analysis and for future Arctic campaign flight and mission planning. The fact that remote sensing alone cannot further constrain model results due to uncertainities in the interpretation of measurements, underlines the need for synchronous in-situ PSC observations in campaigns.

  7. Detailed measurement on a HESCO diffuser

    DEFF Research Database (Denmark)

    Jensen, Rasmus Lund; Holm, Dorte; Nielsen, Peter V.

    2007-01-01

    This paper focuses on measuring the inlet velocity from a HESCO diffuser used in the IEA Annex 20 work as a function of the volume flow it provides. The aim of the present work is to establish a relation between the inlet velocity, the effective area and the airflow. This is important because...... the inlet velocity is a very important boundary condition both in CFD calculation and general flow measurements. If only the volume flow and the geometrical area are used, a relatively large error in the inlet velocity may result. From the detailed measurements it was possible to establish an expression...

  8. A detailed phylogeny for the Methanomicrobiales

    Science.gov (United States)

    Rouviere, P.; Mandelco, L.; Winker, S.; Woese, C. R.

    1992-01-01

    The small subunit rRNA sequence of twenty archaea, members of the Methanomicrobiales, permits a detailed phylogenetic tree to be inferred for the group. The tree confirms earlier studies, based on far fewer sequences, in showing the group to be divided into two major clusters, temporarily designated the "methanosarcina" group and the "methanogenium" group. The tree also defines phylogenetic relationships within these two groups, which in some cases do not agree with the phylogenetic relationships implied by current taxonomic names--a problem most acute for the genus Methanogenium and its relatives. The present phylogenetic characterization provides the basis for a consistent taxonomic restructuring of this major methanogenic taxon.

  9. Detailed radon emanation mapping in Northern Latium

    International Nuclear Information System (INIS)

    Detailed radon surveys over 5,000 km2 of Northern Latium, covering the northern part of the volcanic province of Central Italy, commenced in the mid eighties as part of a geothermal exploration programme; the surveys have subsequently been continued and amplified with environmental protection in mind. The area is now covered by ground emission maps, radon levels in water supplies, emissions from the different lithologies and concentrations in houses. The high uraniferous content of the volcanics, the porous nature of the ubiquitous pyroclastics, and active geothermal systems in the area combine to convey to ground level high concentrations of radon. The emissions show strong lateral variations which are geologically and tectonically controlled, such that only detailed surveys reveal the extent and locations of anomalous radon emanations. Unfortunately, long ago towns often developed in strategic locations. For Northern Latium this means on volcanic highs formed by faulted tuff blocks, two geological features associated with particularly high radon emissions. As a result, in contrast to the low average indoor radon concentrations for the greater part of Italy, in some of these town the average values exceed 450 Bq/m3. (author). 1 fig

  10. Broken detailed balance in active fluctuations of semiflexible filaments

    Science.gov (United States)

    Gladrow, Jannes; Fakhri, Nikta; Mackintosh, Fred C.; Schmidt, Christoph F.; Broedersz, Chase P.

    2015-03-01

    Non-equilibrium microscopic force generation in cells often results in stochastic steady-state fluctuations. In the cell cytoskeleton, for example, cytoplasmic myosins can drive vigorous conformational fluctuations of actin filaments and microtubules. We here present an analytical and numerical analysis of randomly driven shape fluctuations of semiflexible filaments in a viscoelastic environment. To detect and quantify non-equilibrium dynamics, we focus on the breaking of detailed balance in a conformational phase space subtended by eigenmodes of the beam equation. Molecular dynamics simulations reveal a non-zero circulatory flux in phase space induced by motor activity. Furthermore, we derived an analytical expression of nonequilibrium mode correlations that allows us to predict temporal effects of active molecular motors.

  11. Large-scale violation of detailed balance in biological systems

    Science.gov (United States)

    Broedersz, Chase; Battle, Christopher; Fakhri, Nikta; Mackintosh, Fred; Schmidt, Christopher

    2015-03-01

    Living systems are out of equilibrium. A fundamental manifestation of non-equilibrium dynamics in biological systems is the violation of detailed balance: at the microscopic level, enzymatic processes such as kinetic proofreading or molecular motor activity clearly violate detailed balance. We study how such non-equilibrium dynamics emerge at macroscopic scales in cellular assemblies. We measure the steady-state dynamics of two systems, beating flagella of Chlamydomonas reinhardtii and mechanosensitive primary cilia protruding from epithelial kidney cells. The flagellum exhibits clear non-equilibrium driving, whereas fluctuations in the primary cilium are difficult to differentiate from Brownian motion. We parameterize the shapes of the flagellum and primary cilium using a low-dimensional representation of their configuration phase space, and use the measured dynamics to infer the steady-state probability distributions and probability currents. For both the flagellum and the primary cilium we find significant, coherent circulating probability currents, demonstrating that these systems violate detailed balance at the mesoscopic scale.

  12. Detailed Performance Model for Photovoltaic Systems: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Tian, H.; Mancilla-David, F.; Ellis, K.; Muljadi, E.; Jenkins, P.

    2012-07-01

    This paper presents a modified current-voltage relationship for the single diode model. The single-diode model has been derived from the well-known equivalent circuit for a single photovoltaic cell. The modification presented in this paper accounts for both parallel and series connections in an array.

  13. A meaningful expansion around detailed balance

    CERN Document Server

    Colangeli, Matteo; Wynants, Bram

    2011-01-01

    We consider Markovian dynamics modeling open mesoscopic systems which are driven away from detailed balance by a nonconservative force. A systematic expansion is obtained of the stationary distribution around an equilibrium reference, in orders of the nonequilibrium forcing. The first order around equilibrium has been known since the work of McLennan (1959), and involves the transient irreversible entropy flux. The expansion generalizes the McLennan formula to higher orders, complementing the entropy flux with the dynamical activity. The latter is more kinetic than thermodynamic and is a possible realization of Landauer's insight (1975) that, for nonequilibrium, the relative occupation of states also depends on the noise along possible escape routes. In that way nonlinear response around equilibrium can be meaningfully discussed in terms of two main quantities only, the entropy flux and the dynamical activity. The expansion makes mathematical sense as shown in the simplest cases from exponential ergodicity.

  14. Detailed Design of Intelligent Object Framework

    Directory of Open Access Journals (Sweden)

    Sasa Savicand Hao Shi

    2013-12-01

    Full Text Available The design and implementation of Intelligent Object Framework(IOF aims to unite the communication and device management through a platform independent ma nagement protocol in conjunction with a management application. The Core Framework is devel oped using Microsoft Visual Studio, Microsoft’s .NET Framework and Microsoft’s Windows Mobile SDK. Secondary Intelligent Object is developed using Tibbo Integrated Development Environment (TIDE and T-BASIC programming language that is loaded on an EM1026 Embedded Device Platform running Tibbo Op erating System (TiOS. The backend database is based on Microsoft’s SQL Server.In this paper, prot ocols associated with Smart Living are first reviewed.The system architecture and intelligent ob ject management studio are presented. Then device application design and database design are detailed . Finally conclusions are drawn and future work is addressed.

  15. Radioactive contamination mapping system detailed design report

    International Nuclear Information System (INIS)

    The Hanford Site's 100 Area production reactors released radioactively and chemically contaminated liquids into the soil column. The primary source of the contaminated liquids was reactor coolant and various waste waters released from planned liquid discharges, as well as pipelines, pipe junctions, and retention basins leaking into the disposal sites. Site remediation involves excavating the contaminated soils using conventional earthmoving techniques and equipment, treating as appropriate, transporting the soils, and disposing the soils at ERDF. To support remediation excavation, disposal, and documentation requirements, an automated radiological monitoring system was deemed necessary. The RCMS (Radioactive Contamination Mapping System) was designed to fulfill this need. This Detailed Design Report provides design information for the RCMS in accordance with Bechtel Hanford, Inc. Engineering Design Project Instructions

  16. Detailed Chromospheric Activity Nature of KIC 9641031

    CERN Document Server

    Yoldaş, Ezgi

    2016-01-01

    This study depends on KIC 9641031 eclipsing binary system with a chromospherically active component. There are three type variations, such as geometrical variations due to eclipses, sinusoidal variations due to the rotational modulations and also flares, in the light curves obtained with the data taken from the Kepler Mission Database. Taking into account results obtained from KIC 9641031's observations in the Kepler Mission Database, we present and discuss the details of chromospheric activity. The sinusoidal light variations due to rotational modulation and the flare events were modelled separately. 92 different data subsets separated using the analytic models described in the literature were modelled separately to obtain the cool spot configuration. It is seen that just one component of the system is chromospherically active star. On this component, there are two active regions separated by about 180 deg longitudinally between the latitudes of +50 deg and +100 deg, whose locations and forms are rapidly cha...

  17. Detailed chemical kinetic oxidation mechanism for a biodiesel surrogate

    Energy Technology Data Exchange (ETDEWEB)

    Herbinet, O; Pitz, W J; Westbrook, C K

    2007-09-20

    A detailed chemical kinetic mechanism has been developed and used to study the oxidation of methyl decanoate, a surrogate for biodiesel fuels. This model has been built by following the rules established by Curran et al. for the oxidation of n-heptane and it includes all the reactions known to be pertinent to both low and high temperatures. Computed results have been compared with methyl decanoate experiments in an engine and oxidation of rapeseed oil methyl esters in a jet stirred reactor. An important feature of this mechanism is its ability to reproduce the early formation of carbon dioxide that is unique to biofuels and due to the presence of the ester group in the reactant. The model also predicts ignition delay times and OH profiles very close to observed values in shock tube experiments fueled by n-decane. These model capabilities indicate that large n-alkanes can be good surrogates for large methyl esters and biodiesel fuels to predict overall reactivity, but some kinetic details, including early CO{sub 2} production from biodiesel fuels, can be predicted only by a detailed kinetic mechanism for a true methyl ester fuel. The present methyl decanoate mechanism provides a realistic kinetic tool for simulation of biodiesel fuels.

  18. Detailed chemical kinetic oxidation mechanism for a biodiesel surrogate

    Energy Technology Data Exchange (ETDEWEB)

    Herbinet, O; Pitz, W J; Westbrook, C K

    2007-09-17

    A detailed chemical kinetic mechanism has been developed and used to study the oxidation of methyl decanoate, a surrogate for biodiesel fuels. This model has been built by following the rules established by Curran et al. for the oxidation of n-heptane and it includes all the reactions known to be pertinent to both low and high temperatures. Computed results have been compared with methyl decanoate experiments in an engine and oxidation of rapeseed oil methyl esters in a jet stirred reactor. An important feature of this mechanism is its ability to reproduce the early formation of carbon dioxide that is unique to biofuels and due to the presence of the ester group in the reactant. The model also predicts ignition delay times and OH profiles very close to observed values in shock tube experiments fueled by n-decane. These model capabilities indicate that large n-alkanes can be good surrogates for large methyl esters and biodiesel fuels to predict overall reactivity, but some kinetic details, including early CO2 production from biodiesel fuels, can be predicted only by a detailed kinetic mechanism for a true methyl ester fuel. The present methyl decanoate mechanism provides a realistic kinetic tool for simulation of biodiesel fuels.

  19. Probing the structural details of xylan degradation by real-time NMR spectroscopy

    DEFF Research Database (Denmark)

    Petersen, Bent O.; Lok, Finn; Meier, Sebastian

    2014-01-01

    The biodegradation of abundantly available cell wall polysaccharides has recently received much attention, not least because cell wall polysaccharides are substrates for the human gut microbiota and for environmentally sustainable processes of biomass conversion to value-added compounds. A major...... and in products. Reaction progress was visualized for the biodegradation of arabinoxylan by different crude microbial enzyme preparations. The direct observation of structural details in complex mixtures containing arabinoxylan fragments is significant, as such structural details reportedly modulate the health...

  20. Some articulatory details of emotional speech

    Science.gov (United States)

    Lee, Sungbok; Yildirim, Serdar; Bulut, Murtaza; Kazemzadeh, Abe; Narayanan, Shrikanth

    2005-09-01

    Differences in speech articulation among four emotion types, neutral, anger, sadness, and happiness are investigated by analyzing tongue tip, jaw, and lip movement data collected from one male and one female speaker of American English. The data were collected using an electromagnetic articulography (EMA) system while subjects produce simulated emotional speech. Pitch, root-mean-square (rms) energy and the first three formants were estimated for vowel segments. For both speakers, angry speech exhibited the largest rms energy and largest articulatory activity in terms of displacement range and movement speed. Happy speech is characterized by largest pitch variability. It has higher rms energy than neutral speech but articulatory activity is rather comparable to, or less than, neutral speech. That is, happy speech is more prominent in voicing activity than in articulation. Sad speech exhibits longest sentence duration and lower rms energy. However, its articulatory activity is no less than neutral speech. Interestingly, for the male speaker, articulation for vowels in sad speech is consistently more peripheral (i.e., more forwarded displacements) when compared to other emotions. However, this does not hold for female subject. These and other results will be discussed in detail with associated acoustics and perceived emotional qualities. [Work supported by NIH.

  1. Basic and detail engineering development of PTAMB

    International Nuclear Information System (INIS)

    The purpose of the future Treatment and Conditioning of Medium and Low Activity Solid and Liquid Waste Plant (PTAMB) of the National Atomic Energy Commission (CNEA) will be to put up medium and low activity solid and liquid waste and to verify the quality of the conditioned waste generated in Ezeiza Atomic Center (CAE), Constituyentes Atomic Center (CAC) and other national producers outside CNEA. The PTAMB is a Class I Radioactive Installation (according to Basic Standard AR 10.1.1, Rev. 3 RNA, paragraphs 17 and 22) also called Relevant Installation. The aim of this document is to list the steps that carried out the Projects Department of the National Program of Radioactive Waste Management (PNGRR) to arrive to the realization of the detailed engineering of the plant. The project is in Public Tender stage and the beginning of the construction would be March 2010. Once built, the Plant will process the radioactive waste contained in the conceptual engineering, offering more precise control of these and their compatibility with the new final disposal systems to build. (author)

  2. Level of detail technique for plant models

    Institute of Scientific and Technical Information of China (English)

    Xiaopeng ZHANG; Qingqiong DENG; Marc JAEGER

    2006-01-01

    Realistic modelling and interactive rendering of forestry and landscape is a challenge in computer graphics and virtual reality. Recent new developments in plant growth modelling and simulation lead to plant models faithful to botanical structure and development, not only representing the complex architecture of a real plant but also its functioning in interaction with its environment. Complex geometry and material of a large group of plants is a big burden even for high performances computers, and they often overwhelm the numerical calculation power and graphic rendering power. Thus, in order to accelerate the rendering speed of a group of plants, software techniques are often developed. In this paper, we focus on plant organs, i.e. leaves, flowers, fruits and inter-nodes. Our approach is a simplification process of all sparse organs at the same time, i. e. , Level of Detail (LOD) , and multi-resolution models for plants. We do explain here the principle and construction of plant simplification. They are used to construct LOD and multi-resolution models of sparse organs and branches of big trees. These approaches take benefit from basic knowledge of plant architecture, clustering tree organs according to biological structures. We illustrate the potential of our approach on several big virtual plants for geometrical compression or LOD model definition. Finally we prove the efficiency of the proposed LOD models for realistic rendering with a virtual scene composed by 184 mature trees.

  3. Theory including future not excluded

    DEFF Research Database (Denmark)

    Nagao, K.; Nielsen, H.B.

    2013-01-01

    , Ehrenfest's theorem, and the conserved probability current density. In addition,we showthat the expectation value at the present time t of a future-included theory for large T - t and large t - T corresponds to that of a future-not-included theory with a proper inner product for large t - T. Hence, the CAT......We study a complex action theory (CAT) whose path runs over not only past but also future. We show that, if we regard a matrix element defined in terms of the future state at time T and the past state at time TA as an expectation value in the CAT, then we are allowed to have the Heisenberg equation...

  4. Comparison between analytical and test results for transformer base details

    International Nuclear Information System (INIS)

    A caveat concerning weak-way bending of sheet metal transformer base details is included in the Generic Implementation Procedure (GIP) for resolution of Unresolved Safety Issue (USI) A-46. The weak-way bending caveat originated from a concern by the Senior Seismic Review and Advisory Panel (SSRAP). Transformer base details have, however, performed satisfactorily during past strong motion earthquakes. A transformer with thin sheet metal base supports without bracing for lateral forces was recently evaluated for seismic adequacy. Simple analyses indicated very high stresses and field conditions (lack of washers) raised the potential for sheet metal pull-through at the anchor bolts. Shake table testing was therefore performed to confirm that transformer base details have much higher seismic capacity than indicated by linear-elastic analysis. This paper will compare the analytically predicted performance of thin sheet metal transformer supports to the actual performance observed in past strong motion earthquakes; laboratory shake table test data is also presented as confirmation of the experience data

  5. Hepatitis C-Induced Hepatitis Flare in a Patient with Non-Hodgkin B-Cell Lymphoma Treated by Rituximab Including Chemotherapy (Rituximab, Cyclophosphamide, Hydroxydaunorubicin, Oncovin - Vincristine, Prednisolone Regimen

    Directory of Open Access Journals (Sweden)

    Asim Ulcay

    2014-06-01

    Full Text Available Hepatitis virus infections can lead to more critical outcomes such as severe hepatic dysfunction, failure and fulminancy in immunosuppressive patients compared to immunocompetent individuals. It is globally accepted that reactivation of both Hepatitis B virus [HBV ] and Hepatitis C virus [HCV] occurs after chemotherapy and antibody treatments of malignant diseases or solid organ/ bone marrow transplant in recipient patients. Especially among B-cell Non Hodgkin Lymphoma [NHL] patients, according to various studies, the seroprevelance of HCV is higher than that of the general population. On the other hand the role of HCV in the pathogenesis and etiology of NHL has been suggested. Today, cytotoxic drugs, corticosteroids, rituximab and hepatotoxic regimens are administered to NHL patients. Specifically, it has been emphasized that the utilization of rituximab [Anti CD20 antibody ] regiments for B-cell NHL patients may result with flares in HCV patients conspicuously. Here, we report the case of an acute flare up due to HCV infection in a patient who underwent a 4 month course of rituximab containing chemotherapy against a B cell NHL [CD20+ ] disease and a dramatic recovery from HCV infection at the end. [Dis Mol Med 2014; 2(3.000: 51-54

  6. Biodiversity conservation including uncharismatic species

    DEFF Research Database (Denmark)

    Muñoz, Joaquin

    2007-01-01

    Recent papers mention ideas on the topics of biodiversity conservation strategies and priorities (Redford et al. 2003; Lamoreux et al. 2006; Rodrı´guez et al. 2006), the current status of biodiversity (Loreau et al. 2006), the obligations of conservation biologists regarding management policies...... (Chapron 2006; Schwartz 2006), and the main threats to biodiversity (including invasive species) (Bawa 2006). I suggest, however, that these articles do not really deal with biodiversity. Rather, they all focus on a few obviously charismatic groups (mammals, birds, some plants, fishes, human culture...

  7. The surgery of peripheral nerves (including tumors)

    DEFF Research Database (Denmark)

    Fugleholm, Kåre

    2013-01-01

    Surgical pathology of the peripheral nervous system includes traumatic injury, entrapment syndromes, and tumors. The recent significant advances in the understanding of the pathophysiology and cellular biology of peripheral nerve degeneration and regeneration has yet to be translated into improved...... surgical techniques and better outcome after peripheral nerve injury. Decision making in peripheral nerve surgery continues to be a complex challenge, where the mechanism of injury, repeated clinical evaluation, neuroradiological and neurophysiological examination, and detailed knowledge of the peripheral...... nervous system response to injury are prerequisite to obtain the best possible outcome. Surgery continues to be the primary treatment modality for peripheral nerve tumors and advances in adjuvant oncological treatment has improved outcome after malignant peripheral nerve tumors. The present chapter...

  8. ACCREDITATION FOR TECHNICAL ABILITIES INCLUDING COMPUTER SKILLS

    Directory of Open Access Journals (Sweden)

    Halit Hami OZ

    2013-01-01

    Full Text Available Sector Skills are defined by state-sponsored, employer-led organizations that cover specific economic sectors in the European Union and other countries in the world to reduce skills gaps and shortages, improve productivity, boost the skills of their sector workforces and improve learning supply. The accreditation and registration systems used by professional bodies raise the profile of the profession. In many countries including the European Union, professional associations are beginning to accept practice-based accreditation, generally as an alternative to their mainstream systems. Besides studying the certain agencies in the European Union for assessing/accreditating practical abilities , Accreditation for practical abilities of Information Communication Technology and Business Management/Language domains developed by Accreditation Council for Practical abilities are also studied in detail as an example to establish a similar agency in Turkey.

  9. Families classification including multiopposition asteroids

    Science.gov (United States)

    Milani, Andrea; Spoto, Federica; Knežević, Zoran; Novaković, Bojan; Tsirvoulis, Georgios

    2016-01-01

    In this paper we present the results of our new classification of asteroid families, upgraded by using catalog with > 500,000 asteroids. We discuss the outcome of the most recent update of the family list and of their membership. We found enough evidence to perform 9 mergers of the previously independent families. By introducing an improved method of estimation of the expected family growth in the less populous regions (e.g. at high inclination) we were able to reliably decide on rejection of one tiny group as a probable statistical fluke. Thus we reduced our current list to 115 families. We also present newly determined ages for 6 families, including complex 135 and 221, improving also our understanding of the dynamical vs. collisional families relationship. We conclude with some recommendations for the future work and for the family name problem.

  10. Detailed Burnup Calculations for Testing Nuclear Data

    Science.gov (United States)

    Leszczynski, F.

    2005-05-01

    A general method (MCQ) has been developed by introducing a microscopic burnup scheme that uses the Monte Carlo calculated fluxes and microscopic reaction rates of a complex system and a depletion code for burnup calculations as a basis for solving nuclide material balance equations for each spatial region in which the system is divided. Continuous energy-dependent cross-section libraries and full 3D geometry of the system can be input for the calculations. The resulting predictions for the system at successive burnup time steps are thus based on a calculation route where both geometry and cross sections are accurately represented, without geometry simplifications and with continuous energy data, providing an independent approach for benchmarking other methods and nuclear data of actinides, fission products, and other burnable absorbers. The main advantage of this method over the classical deterministic methods currently used is that the MCQ System is a direct 3D method without the limitations and errors introduced on the homogenization of geometry and condensation of energy of deterministic methods. The Monte Carlo and burnup codes adopted until now are the widely used MCNP and ORIGEN codes, but other codes can be used also. For using this method, there is need of a well-known set of nuclear data for isotopes involved in burnup chains, including burnable poisons, fission products, and actinides. For fixing the data to be included in this set, a study of the present status of nuclear data is performed, as part of the development of the MCQ method. This study begins with a review of the available cross-section data of isotopes involved in burnup chains for power and research nuclear reactors. The main data needs for burnup calculations are neutron cross sections, decay constants, branching ratios, fission energy, and yields. The present work includes results of selected experimental benchmarks and conclusions about the sensitivity of different sets of cross

  11. Believing details known to have been suggested

    OpenAIRE

    Higham, Philip A.

    1998-01-01

    In 2 experiments, participants viewed a videotape of a simulated armed robbery, later answered misleading questions about it, and then finally completed a source monitoring test. For the test, participants were asked to indicate for each test item whether it was (1) seen in the video only, (2) read about in the questions only, (3) both seen and read about, (4) not remembered or (5) known to have occurred but the source was unclear. The latter response category was included on the test to remo...

  12. Rotator cuff tear: A detailed update

    Directory of Open Access Journals (Sweden)

    Vivek Pandey

    2015-01-01

    Full Text Available Rotator cuff tear has been a known entity for orthopaedic surgeons for more than two hundred years. Although the exact pathogenesis is controversial, a combination of intrinsic factors proposed by Codman and extrinsic factors theorized by Neer is likely responsible for most rotator cuff tears. Magnetic resonance imaging remains the gold standard for the diagnosis of rotator cuff tears, but the emergence of ultrasound has revolutionized the diagnostic capability. Even though mini-open rotator cuff repair is still commonly performed, and results are comparable to arthroscopic repair, all-arthroscopic repair of rotator cuff tear is now fast becoming a standard care for rotator cuff repair. Appropriate knowledge of pathology and healing pattern of cuff, strong and biological repair techniques, better suture anchors, and gradual rehabilitation of postcuff repair have led to good to excellent outcome after repair. As the healing of degenerative cuff tear remains unpredictable, the role of biological agents such as platelet-rich plasma and stem cells for postcuff repair augmentation is still under evaluation. The role of scaffolds in massive cuff tear is also being probed.

  13. Optimization of Processing and Modeling Issues for Thin Film Solar Cell Devices Including Concepts for the Development of Polycrystalline Multijunctions Annual Subcontract Report, 24 August 1999 - 23 August 2000

    Energy Technology Data Exchange (ETDEWEB)

    Birkmire, R. W.; Phillips, J. E.; Shafarman, W. N.; Eser, E.; Hegedus, S. S.; McCandless, B. E.

    2001-11-14

    This report describes the results achieved during Phase I of a three-phase subcontract to develop and understand thin-film solar cell technology associated with CuInSe2 and related alloys, a-Si and its alloys, and CdTe. Modules based on all these thin films are promising candidates to meet DOE long-range efficiency, reliability, and manufacturing cost goals. The critical issues being addressed under this program are intended to provide the science and engineering basis for developing viable commercial processes and to improve module performance. The generic research issues addressed are: (1) quantitative analysis of processing steps to provide information for efficient commercial-scale equipment design and operation; (2) device characterization relating the device performance to materials properties and process conditions; (3) development of alloy materials with different bandgaps to allow improved device structures for stability and compatibility with module design; (4) development of improved window/heterojunction layers and contacts to improve device performance and reliability; and (5) evaluation of cell stability with respect to illumination, temperature, and ambient, and with respect to device structure and module encapsulation.

  14. Optimization of Processing and Modeling Issues for Thin Film Solar Cell Devices Including Concepts for The Development of Polycrystalline Multijunctions: Annual Report; 24 August 1998-23 August 1999

    Energy Technology Data Exchange (ETDEWEB)

    Birkmire, R.W.; Phillips, J.E.; Shafarman, W.N.; Eser, E.; Hegedus, S.S.; McCandless, B.E. (Institute of Energy Conversion)

    2000-08-25

    This report describes results achieved during phase 1 of a three-phase subcontract to develop and understand thin-film solar cell technology associated to CuInSe{sub 2} and related alloys, a-Si and its alloys, and CdTe. Modules based on all these thin films are promising candidates to meet DOE long-range efficiency, reliability, and manufacturing cost goals. The critical issues being addressed under this program are intended to provide the science and engineering basis for the development of viable commercial processes and to improve module performance. The generic research issues addressed are: (1) quantitative analysis of processing steps to provide information for efficient commercial-scale equipment design and operation; (2) device characterization relating the device performance to materials properties and process conditions; (3) development of alloy materials with different bandgaps to allow improved device structures for stability and compatibility with module design; (4) development of improved window/heterojunction layers and contacts to improve device performance and reliability; and (5) evaluation of cell stability with respect to illumination, temperature, and ambient and with respect to device structure and module encapsulation.

  15. Broken Detailed Balance of Filament Dynamics in Active Networks

    Science.gov (United States)

    Gladrow, J.; Fakhri, N.; MacKintosh, F. C.; Schmidt, C. F.; Broedersz, C. P.

    2016-06-01

    Myosin motor proteins drive vigorous steady-state fluctuations in the actin cytoskeleton of cells. Endogenous embedded semiflexible filaments such as microtubules, or added filaments such as single-walled carbon nanotubes are used as novel tools to noninvasively track equilibrium and nonequilibrium fluctuations in such biopolymer networks. Here, we analytically calculate shape fluctuations of semiflexible probe filaments in a viscoelastic environment, driven out of equilibrium by motor activity. Transverse bending fluctuations of the probe filaments can be decomposed into dynamic normal modes. We find that these modes no longer evolve independently under nonequilibrium driving. This effective mode coupling results in nonzero circulatory currents in a conformational phase space, reflecting a violation of detailed balance. We present predictions for the characteristic frequencies associated with these currents and investigate how the temporal signatures of motor activity determine mode correlations, which we find to be consistent with recent experiments on microtubules embedded in cytoskeletal networks.

  16. Broken detailed balance reveals stress heterogeneity in active matter

    CERN Document Server

    Gladrow, J; MacKintosh, F C; Schmidt, C F; Broedersz, C P

    2016-01-01

    Myosin motor proteins drive vigorous steady-state fluctuations in the actin cytoskeleton of cells. Endogenous embedded semiflexible filaments such as microtubules, or added filaments such as single-walled carbon nanotubes are used as novel tools to non-invasively track equilibrium and non-equilibrium fluctuations in such biopolymer networks. Here we analytically calculate shape fluctuations of semiflexible probe filaments in a viscoelastic environment, driven out of equilibrium by motor activity. Transverse bending fluctuations of the probe filaments can be decomposed into dynamic normal modes. We find that these modes no longer evolve independently under non-equilibrium driving. This effective mode coupling results in non-zero circulatory currents in a conformational phase space, reflecting a violation of detailed balance. We present predictions for the characteristic frequencies associated with these currents and investigate how the temporal signatures of motor activity determine mode correlations, which we...

  17. Including Magnetostriction in Micromagnetic Models

    Science.gov (United States)

    Conbhuí, Pádraig Ó.; Williams, Wyn; Fabian, Karl; Nagy, Lesleis

    2016-04-01

    The magnetic anomalies that identify crustal spreading are predominantly recorded by basalts formed at the mid-ocean ridges, whose magnetic signals are dominated by iron-titanium-oxides (Fe3-xTixO4), so called "titanomagnetites", of which the Fe2.4Ti0.6O4 (TM60) phase is the most common. With sufficient quantities of titanium present, these minerals exhibit strong magnetostriction. To date, models of these grains in the pseudo-single domain (PSD) range have failed to accurately account for this effect. In particular, a popular analytic treatment provided by Kittel (1949) for describing the magnetostrictive energy as an effective increase of the anisotropy constant can produce unphysical strains for non-uniform magnetizations. I will present a rigorous approach based on work by Brown (1966) and by Kroner (1958) for including magnetostriction in micromagnetic codes which is suitable for modelling hysteresis loops and finding remanent states in the PSD regime. Preliminary results suggest the more rigorously defined micromagnetic models exhibit higher coercivities and extended single domain ranges when compared to more simplistic approaches.

  18. Statistical Analysis of Detailed 3-D CFD LES Simulations with Regard to CCV Modeling

    Directory of Open Access Journals (Sweden)

    Vítek Oldřich

    2016-06-01

    Full Text Available The paper deals with statistical analysis of large amount of detailed 3-D CFD data in terms of cycle-to-cycle variations (CCVs. These data were obtained by means of LES calculations of many consecutive cycles. Due to non-linear nature of Navier-Stokes equation set, there is a relatively significant CCV. Hence, every cycle is slightly different – this leads to requirement to perform statistical analysis based on ensemble averaging procedure which enables better understanding of CCV in ICE including its quantification. The data obtained from the averaging procedure provides results on different space resolution levels. The procedure is applied locally, i.e., in every cell of the mesh. Hence there is detailed CCV information on local level – such information can be compared with RANS simulations. Next, volume/mass averaging provides information at specific locations – e.g., gap between electrodes of a spark plug. Finally, volume/mass averaging of the whole combustion chamber leads to global information which can be compared with experimental data or results of system simulation tools (which are based on 0-D/1-D approach.

  19. Transfer Effects of Adding Seductive Details to Case-Based Instruction

    Science.gov (United States)

    Abercrombie, Sara

    2013-01-01

    The current research investigates the efficacy of the case-based instructional method for teacher education when seductive details (i.e. interesting but extraneous details) are included or removed. Aspiring teachers (n = 108) learned about principles of writing effective feedback in a text-based lesson without a description of a classroom case (C…

  20. Thermovoltaic semiconductor device including a plasma filter

    Science.gov (United States)

    Baldasaro, Paul F.

    1999-01-01

    A thermovoltaic energy conversion device and related method for converting thermal energy into an electrical potential. An interference filter is provided on a semiconductor thermovoltaic cell to pre-filter black body radiation. The semiconductor thermovoltaic cell includes a P/N junction supported on a substrate which converts incident thermal energy below the semiconductor junction band gap into electrical potential. The semiconductor substrate is doped to provide a plasma filter which reflects back energy having a wavelength which is above the band gap and which is ineffectively filtered by the interference filter, through the P/N junction to the source of radiation thereby avoiding parasitic absorption of the unusable portion of the thermal radiation energy.

  1. Obesity Is an Independent Predictor of Poor Survival in Metastatic Breast Cancer: Retrospective Analysis of a Patient Cohort Whose Treatment Included High-Dose Chemotherapy and Autologous Stem Cell Support

    International Nuclear Information System (INIS)

    The purpose of the study was to identify predictors of long-term survival in metastatic breast cancer (MBC). A cohort of 96 patients, who received high-dose chemotherapy with autologous stem cell support (HD-ASCT) as part of their treatment, was analyzed. Percent long-term survival at 10 years was 24.5% (CI 17.2-34.9%) when metastasis was diagnosed and 14.4% (CI 8.7-23.9%) when MBC was diagnosed. Survival was impacted significantly by body mass index (BMI). Median overall survival from initial diagnosis or from time of metastasis for patients with BMIs =30 and >30 (obese) was 7.1 (CI 4.4-8.7) and 3.2 years (2.41-6.75), respectively, or 3.2 or 2.3 years (all P=0.02). Also, obesity was the only independent patient-related predictor of time to metastasis and of survival. While obesity is linked with poor outcomes in earlier stages of breast cancer, this has not been previously reported for MBC

  2. Exploration of networks using overview+detail with constraint-based cooperative layout.

    Science.gov (United States)

    Dwyer, Tim; Marriott, Kim; Schreiber, Falk; Stuckey, Peter; Woodward, Michael; Wybrow, Michael

    2008-01-01

    A standard approach to large network visualization is to provide an overview of the network and a detailed view of a small component of the graph centred around a focal node. The user explores the network by changing the focal node in the detailed view or by changing the level of detail of a node or cluster. For scalability, fast force-based layout algorithms are used for the overview and the detailed view. However, using the same layout algorithm in both views is problematic since layout for the detailed view has different requirements to that in the overview. Here we present a model in which constrained graph layout algorithms are used for layout in the detailed view. This means the detailed view has high-quality layout including sophisticated edge routing and is customisable by the user who can add placement constraints on the layout. Scalability is still ensured since the slower layout techniques are only applied to the small subgraph shown in the detailed view. The main technical innovations are techniques to ensure that the overview and detailed view remain synchronized, and modifying constrained graph layout algorithms to support smooth, stable layout. The key innovation supporting stability are new dynamic graph layout algorithms that preserve the topology or structure of the network when the user changes the focus node or the level of detail by in situ semantic zooming. We have built a prototype tool and demonstrate its use in two application domains, UML class diagrams and biological networks.

  3. The Devil's Hole Is In The Details

    Science.gov (United States)

    Wallace, M. G.

    2012-12-01

    As the granularity of Quaternary paleoclimatic proxy signatures from continental and oceanic sources continues to resolve, increasingly integrated studies such as Shakun et. al. (2012), and Kohfeld and Ridgewell (2009) have emerged, with far-reaching but sometimes conflicting paleo-global climate interpretations. Accordingly, none of the competing empirical and phenomenological narratives regarding the time series of Quaternary temperature patterns fit with sufficient fidelity to the observational record. Among other examples, the Shakun et al. study reviewed and processed 80 proxy sites worldwide for paleotemperature reconstruction, but left out the premier Devil's Hole poxy site in the continental Southwestern U.S. The Devil's Hole site presents a nominally earlier record of an interglacial warming signal (Landwehr and Winograd 2012). Both cite similar data (NOAA, 2005) as confirmation of their competing interpretations. Clearly both cannot be right. Epistemic origins of this apparent conflict may be rooted on the ongoing controversy concerning the importance of orbital forcings to the 100K year Quaternary glacial oscillations. Orbital forcings had been intrinsically posited as the only possible extraterrestrial driver of global temperature cycles over the Quaternary time frame. Yet other extraterrestrial climate forcing parameters are now conceivable. This paper reexamines past 3He marine core measurements conducted on two oceans and two hemispheres, and alternatives to the associated interpretations of researchers Patterson and Farley (1998). This study includes a new phenomenological and empirical exploration of an alternate extraterrestrial Quaternary global climate forcing model. This new interpretation is possible based on improved mapping of the Local Interstellar Medium (ISM), as documented in recent works such as those by Frisch and Mueller (2011). References: Frisch, P.C., and HR Mueller, 2011, Time-Variability in the Interstellar Boundary Conditions of

  4. Assessing Pulmonary Pathology by Detailed Examination of Respiratory Function

    OpenAIRE

    Vaickus, Louis J; Bouchard, Jacqueline; Kim, Jiyoun; Natarajan, Sudha; Remick, Daniel G.

    2010-01-01

    Pulmonary inflammation causes multiple alterations within the lung, including mucus production, recruitment of inflammatory cells, and airway hyperreactivity (AHR). Measurement of AHR by direct, invasive means (eg, mechanical ventilation) or noninvasive techniques, like whole body plethysmography (WBP), assesses the severity of pulmonary inflammation in animal models of inflammatory lung disease. Direct measurement of AHR is acknowledged as the most accurate method for assessing airway mechan...

  5. 46 CFR 178.230 - Stability letter or Certificate of Inspection stability details.

    Science.gov (United States)

    2010-10-01

    ... an emergency; (6) Location, type and amount of fixed ballast; (7) Location and details of foam flotation material; and (8) Maximum weight of portable equipment permitted on the vessel including...

  6. Detailed computation of hot-plasma atomic spectra

    CERN Document Server

    Pain, Jean-Christophe; Blenski, Thomas

    2015-01-01

    We present recent evolutions of the detailed opacity code SCO-RCG which combines statistical modelings of levels and lines with fine-structure calculations. The code now includes the Partially-Resolved-Transition-Array model, which allows one to replace a complex transition array by a small-scale detailed calculation preserving energy and variance of the genuine transition array and yielding improved high-order moments. An approximate method for studying the impact of strong magnetic field on opacity and emissivity was also recently implemented. The Zeeman line profile is modeled by fourth-order Gram-Charlier expansion series, which is a Gaussian multiplied by a linear combination of Hermite polynomials. Electron collisional line broadening is often modeled by a Lorentzian function and one has to calculate the convolution of a Lorentzian with Gram-Charlier distribution for a huge number of spectral lines. Since the numerical cost of the direct convolution would be prohibitive, we propose, in order to obtain t...

  7. Detailed ultraviolet asymptotics for AdS scalar field perturbations

    CERN Document Server

    Evnin, Oleg

    2016-01-01

    We present a range of methods suitable for accurate evaluation of the leading asymptotics for integrals of products of Jacobi polynomials in limits when the degrees of some or all polynomials inside the integral become large. The structures in question have recently emerged in the context of effective descriptions of small amplitude perturbations in anti-de Sitter (AdS) spacetime. The limit of high degree polynomials corresponds in this situation to effective interactions involving extreme short-wavelength modes, whose dynamics is crucial for the turbulent instabilities that determine the ultimate fate of small AdS perturbations. We explicitly apply the relevant asymptotic techniques to the case of a self-interacting probe scalar field in AdS and extract a detailed form of the leading large degree behavior, including closed form analytic expressions for the numerical coefficients appearing in the asymptotics.

  8. In Brief: Report details climate change effects on cultural sites

    Science.gov (United States)

    Zielinski, Sarah

    2007-04-01

    A new report from UNESCO (United Nations Educational, Scientific, and Cultural Organization) details how 26 World Heritage sites could be affected by coming climate changes. The 26 examples, which are meant to be representative of the range of threats to the 830 sites inscribed in the World Heritage List, are divided into five types: archaeological sites, glaciers, historic cities and settlements, marine biodiversity, and terrestrial biodiversity. Some of the examples include the Great Barrier Reef, which is expected to experience more frequent episodes of coral bleaching; Timbuktu in Mali, threatened by desertification; and the Chavín Archaeological Site in the Peruvian Central Andes, one of the earliest and best-known pre-Columbian sites, which could be affected by glacier melting and flooding. The report, ``Case Studies on Climate Change and World Heritage,'' is available at http://whc.unesco.org/documents/publi_climatechange.pdf

  9. Detailed Configuration Calculations for Non-LTE Modeling

    Science.gov (United States)

    Fontes, Christopher J.; Abdallah, Joseph, Jr.; Clark, Robert E. H.; Kilcrease, David P.

    1998-11-01

    We continue our work to explore the feasibility of creating detailed atomic models for radiation-hydrodynamics simulations of ICF applications. By further optimizing our atomic data codes we are able to create non-LTE models with a level of complexity approximately one order of magnitude greater (in size) than previously obtained. We present emissivities for gold which include on the order of 75,000 configurations per temperature-density point. The inclusion of additional configurations has yielded improved results for quantities such as the ion fraction distributions, but the question of spectral convergence is yet unanswered. The creation of still larger models will be discussed as well as comparison with experiment and other theories. The possibility of using these models for in-line simulations will also be discussed.

  10. 46 CFR 70.25-1 - Electrical engineering details.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 3 2010-10-01 2010-10-01 false Electrical engineering details. 70.25-1 Section 70.25-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) PASSENGER VESSELS GENERAL PROVISIONS General Electrical Engineering Requirements § 70.25-1 Electrical engineering details. All electrical engineering details and installations shall...

  11. 46 CFR 188.25-1 - Electrical engineering details.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 7 2010-10-01 2010-10-01 false Electrical engineering details. 188.25-1 Section 188.25-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) OCEANOGRAPHIC RESEARCH VESSELS GENERAL PROVISIONS General Electrical Engineering Requirements § 188.25-1 Electrical engineering details. (a) The electrical engineering details...

  12. 46 CFR 90.25-1 - Electrical engineering details.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 4 2010-10-01 2010-10-01 false Electrical engineering details. 90.25-1 Section 90.25-1 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) CARGO AND MISCELLANEOUS VESSELS GENERAL PROVISIONS General Electrical Engineering Requirements § 90.25-1 Electrical engineering details. (a) All electrical engineering details...

  13. Detailed experimental characterization of a reformate fuelled PEM stack

    DEFF Research Database (Denmark)

    Korsgaard, Anders; Nielsen, Mads Pagh; Kær, Søren Knudsen

    2006-01-01

    integrators. Additionally, the paper contains a comprehensive set of test results based on a commercial reformate PEM stack  A series of different synthesis gas compositions were applied to the stack including 5 and 10 ppm CO content, 20% CO2 and air bleeding. During these tests, the dynamic response...... with electric power output from 1-3-kW. All process inputs for the stack can be altered to provide realistic performance analyses, corresponding to those encountered in field applications. These include cathode/anode dew point control, cathode flow rate, cooling water temperature control as well as synthesis...... gas mixing (CO, CO2, N2, Air and H2). The control system includes 12 thermocouple inputs, up to 60 cell voltages, more than 10 flow measurements and 10 pressure measurements, all at sample rates up to 1 kHz. The system design is thoroughly explained to provide valuable information for system...

  14. Computer aided design of reentrant coaxial filters including coaxial excitation

    NARCIS (Netherlands)

    Boria, V.; Gerini, G.; Guglielmi, M.

    1999-01-01

    An advanced EM based CAD tool is used for the detailed characterisation of a family of reentrant coaxial waveguide filters. The EM analysis includes the effects of tuning screws and of the input/output coaxial excitation. The software is essentially used as an efficient replacement for the tradition

  15. Detailed analysis of the promoter activity of an attenuated lentivirus.

    Science.gov (United States)

    Blatti-Cardinaux, Laure; Sanjosé, Leticia; Zahno, Marie-Luise; Zanoni, Reto; Reina, Ramses; Bertoni, Giuseppe

    2016-07-01

    In spite of an eradication campaign that eliminated clinical cases of caprine arthritis encephalitis virus-induced arthritis in the Swiss goat population, seroconversions are still observed. In the affected flocks, viruses belonging mainly to the small ruminant lentivirus A4 subtype are regularly isolated. These viruses are considered attenuated, except in the mammary gland, where high viral loads and histopathological lesions have been observed. We previously characterized and sequenced such field isolates, detecting several potentially attenuating mutations in their LTR. Here we present a detailed analysis of the promoter activity of these genetic elements, which was comparable to those of virulent isolates. An AP-1 binding site was shown to be crucial for promoter activity in reporter gene assays and also in the context of a replicating molecular clone. Other sites, such as AML(vis) and a conserved E-box, appeared to be less crucial. Analysis of a unique AP-4 site showed a clear discrepancy between results obtained with reporter gene assays and those with mutated viruses. Within the limits of this in vitro study, we did not find evidence pointing to the LTR as the genetic correlate of attenuation for these viruses. Finally, the limited replication of SRLV A4 in mammary cell culture could not explain the suggested mammary tropism. In contrast, and in view of the abundance of macrophages in the mammary gland, it is the striking replication capacity of SRLV A4 in these cells, unaffected by all LTR mutations tested, which may explain the apparent mammary tropism of these viruses. PMID:27114068

  16. Detailed pharmacognostical studies on Berberis aristata DC plant

    Directory of Open Access Journals (Sweden)

    Bhawana Rathi

    2013-01-01

    Full Text Available Background: Berberis aristata DC (Berberidaceae commonly known in Hindi as "Dāruhaldi" and "Citra," is an important medicinal herb native to Northern Himalaya region. The plant is used traditionally in Indian system of medicine as an antibacterial, antiperiodic, antidiarrheal and anticancer and it is also used in the treatment of ophthalmic infections. Its root, stem and leaves also find their use in treatment of various ailments and hence is used extensively in Ayurveda. Materials and Methods: Samples of the whole plants of B. aristata were collected and identified. Hand and microtome sections were taken, stained and mounted and the cell content and cell wall structure were studied according to the method described by Kay and Johansen. Representative sketches were made with the help of camera Lucida. Methods for determining the quantitative values were the same as described elsewhere. For fluorescence analysis, the powder of the root, stem and leaf were examined under ultraviolet light. Total ash, acid insoluble ash and water-soluble ash values and water- and alcohol-soluble extractives were determined. Results: The detailed investigations carried on the pharmacognosy of the root; stem and leaf of B. aristata have brought out some salient diagnostic features, which allow one to differentiate it from other substitutes and or adulterants. The determination of quantitative values, fluorescence analysis and the use of lycopodium spore analysis has specifically contributed to this differentiation. Conclusion: From the foregoing observation on the pharmacognosy of root, stem and leaf of B. aristata DC, the salient diagnostic characters of three parts have been presented, which can allow one to differentiate it from other substitutes and or adulterants.

  17. Jacobian transformed and detailed balance approximations for photon induced scattering

    International Nuclear Information System (INIS)

    Photon emission and scattering are enhanced by the number of photons in the final state, and the photon transport equation reflects this in scattering-emission kernels and source terms. This is often a complication in both theoretical and numerical analyzes, requiring approximations and assumptions about background and material temperatures, incident and exiting photon energies, local thermodynamic equilibrium, plus other related aspects of photon scattering and emission. We review earlier schemes parameterizing photon scattering-emission processes, and suggest two alternative schemes. One links the product of photon and electron distributions in the final state to the product in the initial state by Jacobian transformation of kinematical variables (energy and angle), and the other links integrands of scattering kernels in a detailed balance requirement for overall (integrated) induced effects. Compton and inverse Compton differential scattering cross sections are detailed in appropriate limits, numerical integrations are performed over the induced scattering kernel, and for tabulation induced scattering terms are incorporated into effective cross sections for comparisons and numerical estimates. Relativistic electron distributions are assumed for calculations. Both Wien and Planckian distributions are contrasted for impact on induced scattering as LTE limit points. We find that both transformed and balanced approximations suggest larger induced scattering effects at high photon energies and low electron temperatures, and smaller effects in the opposite limits, compared to previous analyzes, with 10-20% increases in effective cross sections. We also note that both approximations can be simply implemented within existing transport modules or opacity processors as an additional term in the effective scattering cross section. Applications and comparisons include effective cross sections, kernel approximations, and impacts on radiative transport solutions in 1D

  18. Detailed model for practical pulverized coal furnaces and gasifiers

    Energy Technology Data Exchange (ETDEWEB)

    Smith, P.J.; Smoot, L.D.

    1989-08-01

    This study has been supported by a consortium of nine industrial and governmental sponsors. Work was initiated on May 1, 1985 and completed August 31, 1989. The central objective of this work was to develop, evaluate and apply a practical combustion model for utility boilers, industrial furnaces and gasifiers. Key accomplishments have included: Development of an advanced first-generation, computer model for combustion in three dimensional furnaces; development of a new first generation fouling and slagging submodel; detailed evaluation of an existing NO{sub x} submodel; development and evaluation of an improved radiation submodel; preparation and distribution of a three-volume final report: (a) Volume 1: General Technical Report; (b) Volume 2: PCGC-3 User's Manual; (c) Volume 3: Data Book for Evaluation of Three-Dimensional Combustion Models; and organization of a user's workshop on the three-dimensional code. The furnace computer model developed under this study requires further development before it can be applied generally to all applications; however, it can be used now by specialists for many specific applications, including non-combusting systems and combusting geseous systems. A new combustion center was organized and work was initiated to continue the important research effort initiated by this study. 212 refs., 72 figs., 38 tabs.

  19. Hydrodynamics of interaction of particles (including cells) with surfaces

    Science.gov (United States)

    Duszyk, Marek; Doroszewski, Jan

    The study of the phenomena related to the motion of particles flowing in the proximity of the wall is pursued for purely cognitive reason as well as for some important practical purposes in various fields of technology, biology and medicine. When small spherical rigid particles move in the direction parallel to the surface their velocity is smaller than that of the fluid and depends on the ratio of the distance from the wall to the particle radius. The velocity of a particle falling down in a vertical cylinder is maximal in an eccentric position. A sphere in contact with the wall remains stationary. Translational velocity of spherical rigid particles the dimension of which are comparable to that of the tube is only slightly dependent of their lateral position. The differences in the flow parameters of deformable particles in comparison with rigid ones depend on the particle and fluid viscosity coefficient. When the particles move perpendicularly toward the wall, their velocity decreases as the particle approaches the surface. The change of particle velocity is inversely proportional to the gap. There are several theories explaining the influence of the channel diameter on the suspension viscosity (sigma phenomenon); a modern approach is based on the analysis of rheological properties of suspensions. The explanations of the Fahraeus effect (i.e. the fact that the concentration of particles flowing in a tube linking two containers are smaller than that in the containers) are based on non-uniform particle distribution in a transverse cross section and on the differences of velocities of particles and medium. The deviation of the velocity profile of a suspension of rigid particles flowing through a tube from the parabolic shape (blunting) does not depend on the flow velocity; as concerns deformable particles, however, this effect is the smaller the greater is the flow velocity. When the Reynolds number for particles is greater than 10 -3, there appears a component of particle velocity perpendicular to the streamline direction. This phenomenon is the cause of the lateral migration of particles. Neutrally buoyant rigid particles migrate to a certain concentrical region situated between the tube axis and the wall (tubular pinch region). Deformable neutrally buoyant particles migrate towards the tube axis, and deformable non-neutrally buoyant particles may move either toward the tube axis or toward the wall. In the research on the influence of the flow delimiting surface on the motion of particles in suspension a considerable progress has recently been made. However, the phenomena in this field are extremely complex. At present, two main types of approach may be distinguished. On a microscopic level direct interactions between particles and surfaces are analyzed. A macroscopic approach consists in treating particle suspension as fluid, and overall influence of the surface on its properties are studied. A comprehensive theory linking these two levels has not yet emerged.

  20. General Information about Plasma Cell Neoplasms (Including Multiple Myeloma)

    Science.gov (United States)

    ... or certain chemicals . Tests that examine the blood, bone marrow, and urine are used to detect (find) and ... amyloidosis. Radiation therapy : Radiation therapy is given for bone lesions of the spine . Chemotherapy: Chemotherapy is given to ...

  1. Treatment Option Overview (Plasma Cell Neoplasms Including Multiple Myeloma)

    Science.gov (United States)

    ... or certain chemicals . Tests that examine the blood, bone marrow, and urine are used to detect (find) and ... amyloidosis. Radiation therapy : Radiation therapy is given for bone lesions of the spine . Chemotherapy: Chemotherapy is given to ...

  2. Research on the Hotel Image Based on the Detail Service

    Science.gov (United States)

    Li, Ban; Shenghua, Zheng; He, Yi

    Detail service management, initially developed as marketing programs to enhance customer loyalty, has now become an important part of customer relation strategy. This paper analyzes the critical factors of detail service and its influence on the hotel image. We establish the theoretical model of influencing factors on hotel image and propose corresponding hypotheses. We use applying statistical method to test and verify the above-mentioned hypotheses. This paper provides a foundation for further study of detail service design and planning issues.

  3. A detailed view of the intracellular transcriptome of Listeria monocytogenes in murine macrophages using RNA-seq

    Directory of Open Access Journals (Sweden)

    Tilman Gunter Schultze

    2015-10-01

    Full Text Available Listeria monocytogenes is a bacterial pathogen and causative agent for the foodborne infection listeriosis, which is mainly a threat for pregnant, elderly or immunocompromised individuals. Due to its ability to invade and colonize diverse eukaryotic cell types including cells from invertebrates, L. monocytogenes has become a well-established model organism for intracellular growth. Almost ten years ago, we and others presented the first whole-genome microarray-based intracellular transcriptome of L. monocytogenes. With the advent of newer technologies addressing transcriptomes in greater detail, we revisit this work, and analyze the intracellular transcriptome of L. monocytogenes during growth in murine macrophages using a deep sequencing based approach.We detected 656 differentially expressed genes of which 367 were upregulated during intracellular growth in macrophages compared to extracellular growth in BHI. This study confirmed ~64% of all regulated genes previously identified by microarray analysis. Many of the regulated genes that were detected in the current study involve transporters for various metals, ions as well as complex sugars such as mannose. We also report changes in antisense transcription, especially upregulations during intracellular bacterial survival. A notable finding was the detection of regulatory changes for a subset of temperate A118-like prophage genes, thereby shedding light on the transcriptional profile of this bacteriophage during intracellular growth. In total, our study provides an updated genome-wide view of the transcriptional landscape of L. monocytogenes during intracellular growth and represents a rich resource for future detailed analysis.

  4. Combustion instability detection using the wavelet detail of pressure fluctuations

    Institute of Scientific and Technical Information of China (English)

    Junjie JI; Yonghao LUO

    2008-01-01

    A combustion instability detection method that uses the wavelet detail of combustion pressure fluctuations is put forward. To confirm this method, combustion pressure fluctuations in a stoker boiler are recorded at stable and unstable combustion with a pressure transducer. Daubechies one-order wavelet is chosen to obtain the wavelet details for comparison. It shows that the wavelet approximation indicates the general pressure change in the furnace, and the wavelet detail magnitude is consistent with the intensity of turbulence and combustion noise. The magnitude of the wavelet detail is nearly constant when the combustion is stable, however, it will fluctuate much when the combustion is unstable.

  5. Lunar Surface Access Module Descent Engine Turbopump Technology: Detailed Design

    Science.gov (United States)

    Alvarez, Erika; Forbes, John C.; Thornton, Randall J.

    2010-01-01

    The need for a high specific impulse LOX/LH2 pump-fed lunar lander engine has been established by NASA for the new lunar exploration architecture. Studies indicate that a 4-engine cluster in the thrust range of 9,000-lbf each is a candidate configuration for the main propulsion of the manned lunar lander vehicle. The lander descent engine will be required to perform multiple burns including the powered descent onto the lunar surface. In order to achieve the wide range of thrust required, the engines must be capable of throttling approximately 10:1. Working under internal research and development funding, NASA Marshall Space Flight Center (MSFC) has been conducting the development of a 9,000-lbf LOX/LH2 lunar lander descent engine technology testbed. This paper highlights the detailed design and analysis efforts to develop the lander engine Fuel Turbopump (FTP) whose operating speeds range from 30,000-rpm to 100,000-rpm. The capability of the FTP to operate across this wide range of speeds imposes several structural and dynamic challenges, and the small size of the FTP creates scaling and manufacturing challenges that are also addressed in this paper.

  6. Detailed 3-D nuclear analysis of ITER blanket modules

    Energy Technology Data Exchange (ETDEWEB)

    Bohm, T.D., E-mail: tdbohm@wisc.edu [University of Wisconsin-Madison, Madison, WI (United States); Sawan, M.E.; Marriott, E.P.; Wilson, P.P.H. [University of Wisconsin-Madison, Madison, WI (United States); Ulrickson, M.; Bullock, J. [Sandia National Laboratories, Albuquerque, NM (United States)

    2014-10-15

    In ITER, the blanket modules (BM) are arranged around the plasma to provide thermal and nuclear shielding for the vacuum vessel (VV), magnets, and other components. As a part of the BM design process, nuclear analysis is required to determine the level of nuclear heating, helium production, and radiation damage in the BM. Additionally, nuclear heating in the VV is also important for assessing the BM design. We used the CAD based DAG-MCNP5 transport code to analyze detailed models inserted into a 40-degree partially homogenized ITER global model. The regions analyzed include BM01, the neutral beam injection (NB) region, and the upper port region. For BM01, the results show that He production meets the limit necessary for re-welding, and the VV heating behind BM01 is acceptable. For the NBI region, the VV nuclear heating behind the NB region exceeds the design limit by a factor of two. For the upper port region, the nuclear heating of the VV exceeds the design limit by up to 20%. The results presented in this work are being used to modify the BM design in the cases where limits are exceeded.

  7. Further ALMA observations and detailed modeling of the Red Rectangle

    CERN Document Server

    Bujarrabal, V; Alcolea, J; Santander-Garcia, M; Van Winckel, H; Contreras, C Sanchez

    2016-01-01

    We present new high-quality ALMA observations of the Red Rectangle (a well known post-AGB object) in C17O J=6-5 and H13CN J=4-3 line emission and results from a new reduction of already published 13CO J=3-2 data. A detailed model fitting of all the molecular line data, including previous maps and single-dish spectra, was performed using a sophisticated code. These observations and the corresponding modeling allowed us to deepen the analysis of the nebular properties. We also stress the uncertainties in the model fitting. We confirm the presence of a rotating equatorial disk and an outflow, which is mainly formed of gas leaving the disk. The mass of the disk is ~ 0.01 Mo, and that of the CO-rich outflow is ~ 10 times smaller. High temperatures of ~ 100 K are derived for most components. From comparison of the mass values, we roughly estimate the lifetime of the rotating disk, which is found to be of about 10000 yr. Taking data of a few other post-AGB composite nebulae into account, we find that the lifetimes o...

  8. Fluctuations, temperature, and detailed balance in classical nucleation theory

    Energy Technology Data Exchange (ETDEWEB)

    McGraw, R. [Environmental Chemistry Division, Brookhaven National Laboratory, Upton, New York 11973 (United States); LaViolette, R.A. [Idaho National Engineering Laboratory, P.O. Box 1625, Idaho Falls, Idaho 83415 (United States)

    1995-06-08

    The role of temperature in classical nucleation theory is examined. It is shown that while even small clusters are assigned a temperature in the classical theory, this must be a fluctuating quantity. Stochastic simulations of cluster evaporation and growth are presented to track the temperature fluctuations in time. The relation {l_angle}{vert_bar}{delta}{ital T}{vert_bar}{sup 2}{r_angle}={ital kT}{sup @2}{ital d}0/{ital C}{sub {nu}} for the mean square temperature fluctuation is confirmed, where {ital k} is the Boltzmann constant, {ital C}{sub {nu}} is the cluster heat capacity, and {ital T}{sub 0} is the bath temperature. For small capillary drops (50--100 molecules), the resulting rms temperature fluctuations of 10{degree}--20{degree} might be expected to have a significant effect on the nucleation rate. However, the simulations reveal a cluster temperature distribution that is centered several degrees below {ital T}{sub 0}. A theory is presented to explain this effect. To first order, which includes Gaussian fluctuations of the cluster temperature {ital T}, we find that the effective temperature for cluster evaporation is {ital T}{minus}{ital h}/2{ital C}{sub {nu}}, where {ital h} is the latent heat. This temperature correction is precisely that required by detailed balance and results both in a centering of the cluster temperature distribution on {ital T}{sub 0} and a cancellation of any significant effect of temperature fluctuations on the nucleation rate.

  9. Detailed Evaluation of MODIS Fire Radiative Power Measurements

    Science.gov (United States)

    Ichoku, Charles

    2010-01-01

    Satellite remote sensing is providing us tremendous opportunities to measure the fire radiative energy (FRE) release rate or power (FRP) from open biomass burning, which affects many vegetated regions of the world on a seasonal basis. Knowledge of the biomass burning characteristics and emission source strengths of different (particulate and gaseous) smoke constituents is one of the principal ingredients upon which the assessment, modeling, and forecasting of their distribution and impacts depend. This knowledge can be gained through accurate measurement of FRP, which has been shown to have a direct relationship with the rates of biomass consumption and emissions of major smoke constituents. Over the last decade or so, FRP has been routinely measured from space by both the MODIS sensors aboard the polar orbiting Terra and Aqua satellites, and the SEVIRI sensor aboard the Meteosat Second Generation (MSG) geostationary satellite. During the last few years, FRP has been gaining recognition as an important parameter for facilitating the development of various scientific studies relating to the quantitative characterization of biomass burning and their emissions. Therefore, we are conducting a detailed analysis of the FRP products from MODIS to characterize the uncertainties associated with them, such as those due to the MODIS bow-tie effects and other factors, in order to establish their error budget for use in scientific research and applications. In this presentation, we will show preliminary results of the MODIS FRP data analysis, including comparisons with airborne measurements.

  10. Accounting for highly excited states in detailed opacity calculations

    CERN Document Server

    Pain, Jean-Christophe

    2015-01-01

    In multiply-charged ion plasmas, a significant number of electrons may occupy high-energy orbitals. These "Rydberg" electrons, when they act as spectators, are responsible for a number of satellites of X-ray absorption or emission lines, yielding a broadening of the red wing of the resonance lines. The contribution of such satellite lines may be important, because of the high degeneracy of the relevant excited configurations which give these large Boltzmann weights. However, it is in general difficult to take these configurations into account since they are likely to give rise to a large number of lines. We propose to model the perturbation induced by the spectators in a way similar to the Partially-Resolved-Transition-Array approach recently published by C. Iglesias. It consists in a partial detailed-line-accounting calculation in which the effect of the Rydberg spectators is included through a shift and width, expressed in terms of the canonical partition functions, which are key-ingredients of the Super-Tr...

  11. 46 CFR 56.75-30 - Pipe joining details.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Pipe joining details. 56.75-30 Section 56.75-30 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Brazing § 56.75-30 Pipe joining details. (a) Silver brazing. (1) Circumferential pipe joints...

  12. 46 CFR 56.75-25 - Detail requirements.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 2 2010-10-01 2010-10-01 false Detail requirements. 56.75-25 Section 56.75-25 Shipping COAST GUARD, DEPARTMENT OF HOMELAND SECURITY (CONTINUED) MARINE ENGINEERING PIPING SYSTEMS AND APPURTENANCES Brazing § 56.75-25 Detail requirements. (a) Pipe may be fabricated by brazing when the...

  13. ZBLAN supercontinuum generation - detailed comparison between measurement and simulation

    DEFF Research Database (Denmark)

    Agger, Christian; Petersen, Christian; Dupont, Sune;

    2011-01-01

    We present a detailed comparison between modeling and experiments on supercontinuum generation in a ZBLAN fiber. Good agreement is obtained when pumping both in the normal and anomalous dispersion regimes.......We present a detailed comparison between modeling and experiments on supercontinuum generation in a ZBLAN fiber. Good agreement is obtained when pumping both in the normal and anomalous dispersion regimes....

  14. Commentary: The Perils of Seduction: Distracting Details or Incomprehensible Abstractions?

    Science.gov (United States)

    Goetz, Ernest T.; Sadoski, Mark

    1995-01-01

    Reviews studies that have explicitly investigated the "seductive detail" effect (in which a reader's attention is diverted toward the interesting but unimportant seductive details and away from the uninteresting but important main ideas). Concludes that these studies do not provide convincing evidence for the existence of the effect. Argues that…

  15. 44 CFR 5.27 - Deletion of identifying details.

    Science.gov (United States)

    2010-10-01

    ... 44 Emergency Management and Assistance 1 2010-10-01 2010-10-01 false Deletion of identifying details. 5.27 Section 5.27 Emergency Management and Assistance FEDERAL EMERGENCY MANAGEMENT AGENCY..., FEMA may delete identifying details when making available or publishing an opinion, statement of...

  16. Space Telecommunications Radio System (STRS) Architecture, Tutorial Part 2 - Detailed

    Science.gov (United States)

    Handler, Louis

    2014-01-01

    The STRS architecture detail presentation presents each requirement in the STRS Architecture Standard with some examples and supporting information. The purpose is to give a platform provider, application provider, or application integrator a better, more detailed understanding of the STRS Architecture Standard and its use.

  17. Detailed source process of the 2007 Tocopilla earthquake.

    Science.gov (United States)

    Peyrat, S.; Madariaga, R.; Campos, J.; Asch, G.; Favreau, P.; Bernard, P.; Vilotte, J.

    2008-05-01

    We investigated the detail rupture process of the Tocopilla earthquake (Mw 7.7) of the 14 November 2007 and of the main aftershocks that occurred in the southern part of the North Chile seismic gap using strong motion data. The earthquake happen in the middle of the permanent broad band and strong motion network IPOC newly installed by GFZ and IPGP, and of a digital strong-motion network operated by the University of Chile. The Tocopilla earthquake is the last large thrust subduction earthquake that occurred since the major Iquique 1877 earthquake which produced a destructive tsunami. The Arequipa (2001) and Antofagasta (1995) earthquakes already ruptured the northern and southern parts of the gap, and the intraplate intermediate depth Tarapaca earthquake (2005) may have changed the tectonic loading of this part of the Peru-Chile subduction zone. For large earthquakes, the depth of the seismic rupture is bounded by the depth of the seismogenic zone. What controls the horizontal extent of the rupture for large earthquakes is less clear. Factors that influence the extent of the rupture include fault geometry, variations of material properties and stress heterogeneities inherited from the previous ruptures history. For subduction zones where structures are not well known, what may have stopped the rupture is not obvious. One crucial problem raised by the Tocopilla earthquake is to understand why this earthquake didn't extent further north, and at south, what is the role of the Mejillones peninsula that seems to act as a barrier. The focal mechanism was determined using teleseismic waveforms inversion and with a geodetic analysis (cf. Campos et al.; Bejarpi et al., in the same session). We studied the detailed source process using the strong motion data available. This earthquake ruptured the interplate seismic zone over more than 150 km and generated several large aftershocks, mainly located south of the rupture area. The strong-motion data show clearly two S

  18. Investigating the Interaction of Graphic Organizers and Seductive Details: Can a Graphic Organizer Mitigate the Seductive-Details Effect?

    Science.gov (United States)

    Rowland-Bryant, Emily; Skinner, Christopher H.; Skinner, Amy L.; Saudargas, Richard; Robinson, Daniel H.; Kirk, Emily R.

    2009-01-01

    The interaction between seductive details (SD) and a graphic organizer (GO) was investigated. Undergraduate students (n = 207) read a target-material passage about Freud's psychosexual stages. Depending on condition, the participants also read a biographical paragraph (SD-only), viewed a graphic organizer that linked the seductive details to the…

  19. Recovering and Preventing Loss of Detailed Memory: Differential Rates of Forgetting for Detail Types in Episodic Memory

    Science.gov (United States)

    Sekeres, Melanie J.; Bonasia, Kyra; St-Laurent, Marie; Pishdadian, Sara; Winocur, Gordon; Grady, Cheryl; Moscovitch, Morris

    2016-01-01

    Episodic memories undergo qualitative changes with time, but little is known about how different aspects of memory are affected. Different types of information in a memory, such as perceptual detail, and central themes, may be lost at different rates. In patients with medial temporal lobe damage, memory for perceptual details is severely impaired,…

  20. Detailed Hydrographic Feature Extraction from High-Resolution LiDAR Data

    Energy Technology Data Exchange (ETDEWEB)

    Danny L. Anderson

    2012-05-01

    Detailed hydrographic feature extraction from high-resolution light detection and ranging (LiDAR) data is investigated. Methods for quantitatively evaluating and comparing such extractions are presented, including the use of sinuosity and longitudinal root-mean-square-error (LRMSE). These metrics are then used to quantitatively compare stream networks in two studies. The first study examines the effect of raster cell size on watershed boundaries and stream networks delineated from LiDAR-derived digital elevation models (DEMs). The study confirmed that, with the greatly increased resolution of LiDAR data, smaller cell sizes generally yielded better stream network delineations, based on sinuosity and LRMSE. The second study demonstrates a new method of delineating a stream directly from LiDAR point clouds, without the intermediate step of deriving a DEM. Direct use of LiDAR point clouds could improve efficiency and accuracy of hydrographic feature extractions. The direct delineation method developed herein and termed “mDn”, is an extension of the D8 method that has been used for several decades with gridded raster data. The method divides the region around a starting point into sectors, using the LiDAR data points within each sector to determine an average slope, and selecting the sector with the greatest downward slope to determine the direction of flow. An mDn delineation was compared with a traditional grid-based delineation, using TauDEM, and other readily available, common stream data sets. Although, the TauDEM delineation yielded a sinuosity that more closely matches the reference, the mDn delineation yielded a sinuosity that was higher than either the TauDEM method or the existing published stream delineations. Furthermore, stream delineation using the mDn method yielded the smallest LRMSE.

  1. Ligands, cell-based models, and readouts required for Toll-like receptor action.

    LENUS (Irish Health Repository)

    Dellacasagrande, Jerome

    2012-02-01

    This chapter details the tools that are available to study Toll-like receptor (TLR) biology in vitro. This includes ligands, host cells, and readouts. The use of modified TLRs to circumvent some technical problems is also discussed.

  2. Detailed characterization of Mirafiori lettuce virus-resistant transgenic lettuce.

    Science.gov (United States)

    Kawazu, Yoichi; Fujiyama, Ryoi; Noguchi, Yuji; Kubota, Masaharu; Ito, Hidekazu; Fukuoka, Hiroyuki

    2010-04-01

    Lettuce big-vein disease is caused by Mirafiori lettuce virus (MiLV), which is vectored by the soil-borne fungus Olpidium brassicae. A MiLV-resistant transgenic lettuce line was developed through introducing inverted repeats of the MiLV coat protein (CP) gene. Here, a detailed characterization study of this lettuce line was conducted by comparing it with the parental, non-transformed 'Kaiser' cultivar. There were no significant differences between transgenic and non-transgenic lettuce in terms of pollen fertility, pollen dispersal, seed production, seed dispersal, dormancy, germination, growth of seedlings under low or high temperature, chromatographic patterns of leaf extracts, or effects of lettuce on the growth of broccoli or soil microflora. A significant difference in pollen size was noted, but the difference was small. The length of the cotyledons of the transgenic lettuce was shorter than that of 'Kaiser,' but there were no differences in other morphological characteristics. Agrobacterium tumefaciens used for the production of transgenic lettuce was not detected in transgenic seeds. The transgenic T(3), T(4), and T(5) generations showed higher resistance to MiLV and big-vein symptoms expression than the resistant 'Pacific' cultivar, indicating that high resistance to lettuce big-vein disease is stably inherited. PCR analysis showed that segregation of the CP gene was nearly 3:1 in the T(1) and T(2) generations, and that the transgenic T(3) generation was homozygous for the CP gene. Segregation of the neomycin phosphotransferase II (npt II) gene was about 3:1 in the T(1) generation, but the full length npt II gene was not detected in the T(2) or T(3) generation. The segregation pattern of the CP and npt II genes in the T(1) generation showed the expected 9:3:3:1 ratio. These results suggest that the fragment including the CP gene and that including the npt II gene have been integrated into two unlinked loci, and that the T(1) plant selected in our study did

  3. Data on the descriptive overview and the quality assessment details of 12 qualitative research papers.

    Science.gov (United States)

    Barnabishvili, Maia; Ulrichs, Timo; Waldherr, Ruth

    2016-09-01

    This data article presents the supplementary material for the review paper "Role of acceptability barriers in delayed diagnosis of Tuberculosis: Literature review from high burden countries" (Barnabishvili et al., in press) [1]. General overview of 12 qualitative papers, including the details about authors, years of publication, data source locations, study objectives, overview of methods, study population characteristics, as well as the details of intervention and the outcome parameters of the papers are summarized in the first two tables included to the article. Quality assessment process of the methodological strength of 12 papers and the results of the critical appraisal are further described and summarized in the second part of the article.

  4. An Integrated Biochemistry Laboratory, Including Molecular Modeling

    Science.gov (United States)

    Hall, Adele J. Wolfson Mona L.; Branham, Thomas R.

    1996-11-01

    ) experience with methods of protein purification; (iii) incorporation of appropriate controls into experiments; (iv) use of basic statistics in data analysis; (v) writing papers and grant proposals in accepted scientific style; (vi) peer review; (vii) oral presentation of results and proposals; and (viii) introduction to molecular modeling. Figure 1 illustrates the modular nature of the lab curriculum. Elements from each of the exercises can be separated and treated as stand-alone exercises, or combined into short or long projects. We have been able to offer the opportunity to use sophisticated molecular modeling in the final module through funding from an NSF-ILI grant. However, many of the benefits of the research proposal can be achieved with other computer programs, or even by literature survey alone. Figure 1.Design of project-based biochemistry laboratory. Modules (projects, or portions of projects) are indicated as boxes. Each of these can be treated independently, or used as part of a larger project. Solid lines indicate some suggested paths from one module to the next. The skills and knowledge required for protein purification and design are developed in three units: (i) an introduction to critical assays needed to monitor degree of purification, including an evaluation of assay parameters; (ii) partial purification by ion-exchange techniques; and (iii) preparation of a grant proposal on protein design by mutagenesis. Brief descriptions of each of these units follow, with experimental details of each project at the end of this paper. Assays for Lysozyme Activity and Protein Concentration (4 weeks) The assays mastered during the first unit are a necessary tool for determining the purity of the enzyme during the second unit on purification by ion exchange. These assays allow an introduction to the concept of specific activity (units of enzyme activity per milligram of total protein) as a measure of purity. In this first sequence, students learn a turbidimetric assay

  5. Characteristics and impact of drug detailing for gabapentin.

    Directory of Open Access Journals (Sweden)

    Michael A Steinman

    2007-04-01

    Full Text Available BACKGROUND: Sales visits by pharmaceutical representatives ("drug detailing" are common, but little is known about the content of these visits or about the impact of visit characteristics on prescribing behavior. In this study, we evaluated the content and impact of detail visits for gabapentin by analyzing market research forms completed by physicians after receiving a detail visit for this drug. METHODS AND FINDINGS: Market research forms that describe detail visits for gabapentin became available through litigation that alleged that gabapentin was promoted for "off-label" uses. Forms were available for 97 physicians reporting on 116 detail visits between 1995 and 1999. Three-quarters of recorded visits (91/116 occurred in 1996. Two-thirds of visits (72/107 were 5 minutes or less in duration, 65% (73/113 were rated of high informational value, and 39% (42/107 were accompanied by the delivery or promise of samples. During the period of this study, gabapentin was approved by the US Food and Drug Administration only for the adjunctive treatment of partial seizures, but in 38% of visits (44/115 the "main message" of the visit involved at least one off-label use. After receiving the detail visit, 46% (50/108 of physicians reported the intention to increase their prescribing or recommending of gabapentin in the future. In multivariable analysis, intent to increase future use or recommendation of gabapentin was associated with receiving the detail in a small group (versus one-on-one setting and with low or absent baseline use of the drug, but not with other factors such as visit duration, discussion of "on-label" versus "off-label" content, and the perceived informational value of the presentation. CONCLUSIONS: Detail visits for gabapentin were of high perceived informational value and often involved messages about unapproved uses. Despite their short duration, detail visits were frequently followed by physician intentions to increase their future

  6. Progress in polymer solar cell

    Institute of Scientific and Technical Information of China (English)

    LI LiGui; LU GuangHao; YANG XiaoNiu; ZHOU EnLe

    2007-01-01

    This review outlines current progresses in polymer solar cell. Compared to traditional silicon-based photovoltaic (PV) technology, the completely different principle of optoelectric response in the polymer cell results in a novel configuration of the device and more complicated photovoltaic generation process. The conception of bulk-heterojunction (BHJ) is introduced and its advantage in terms of morphology is addressed. The main aspects including the morphology of photoactive layer, which limit the efficiency and stability of polymer solar cell, are discussed in detail. The solutions to boosting up both the efficiency and stability (lifetime) of the polymer solar cell are highlighted at the end of this review.

  7. Detailed black hole state counting in loop quantum gravity

    Science.gov (United States)

    Agullo, Ivan; Barbero G., J. Fernando; Borja, Enrique F.; Diaz-Polo, Jacobo; Villaseñor, Eduardo J. S.

    2010-10-01

    We give a complete and detailed description of the computation of black hole entropy in loop quantum gravity by employing the most recently introduced number-theoretic and combinatorial methods. The use of these techniques allows us to perform a detailed analysis of the precise structure of the entropy spectrum for small black holes, showing some relevant features that were not discernible in previous computations. The ability to manipulate and understand the spectrum up to the level of detail that we describe in the paper is a crucial step toward obtaining the behavior of entropy in the asymptotic (large horizon area) regime.

  8. Detailed black hole state counting in loop quantum gravity

    CERN Document Server

    Agullo, Ivan; Borja, Enrique F; Diaz-Polo, Jacobo; Villaseñor, Eduardo J S; 10.1103/PhysRevD.82.084029

    2011-01-01

    We give a complete and detailed description of the computation of black hole entropy in loop quantum gravity by employing the most recently introduced number-theoretic and combinatorial methods. The use of these techniques allows us to perform a detailed analysis of the precise structure of the entropy spectrum for small black holes, showing some relevant features that were not discernible in previous computations. The ability to manipulate and understand the spectrum up to the level of detail that we describe in the paper is a crucial step towards obtaining the behavior of entropy in the asymptotic (large horizon area) regime.

  9. Reliability assessment of welded steel details in bridges using inspection

    DEFF Research Database (Denmark)

    Toft, Henrik Stensgaard; Sørensen, John Dalsgaard; Yalamas, T.;

    2014-01-01

    membrane stresses are estimated using a generic bridge structure and traffic measurements. The optimal reliability level for a welded detail in a bridge subjected to fatigue are estimated by cost benefit-analysis taking into account the risk of human lives through the Life Quality Index. Since the optimal......This paper presents a bilinear two-dimensional probabilistic fracture mechanics model for fatigue crack growth in welded steel details. The model takes besides membrane stresses also the influence of misalignments and bending stresses in the welded detail into account. The distribution of the...

  10. Lightning climatology in the Congo Basin: detailed analysis

    Science.gov (United States)

    Soula, Serge; Kigotsi, Jean; Georgis, Jean-François; Barthe, Christelle

    2016-04-01

    The lightning climatology of the Congo Basin including several countries of Central Africa is analyzed in detail for the first time. It is based on World Wide Lightning Location Network (WWLLN) data for the period from 2005 to 2013. A comparison of these data with the Lightning Imaging Sensor (LIS) data for the same period shows the WWLLN detection efficiency (DE) in the region increases from about 1.70 % in the beginning of the period to 5.90 % in 2013, relative to LIS data, but not uniformly over the whole 2750 km × 2750 km area. Both the annual flash density and the number of stormy days show sharp maximum values localized in eastern of Democratic Republic of Congo (DRC) and west of Kivu Lake, regardless of the reference year and the period of the year. These maxima reach 12.86 fl km-2 and 189 days, respectively, in 2013, and correspond with a very active region located at the rear of the Virunga mountain range characterised with summits that can reach 3000 m. The presence of this range plays a role in the thunderstorm development along the year. The estimation of this local maximum of the lightning density by taking into account the DE, leads to a value consistent with that of the global climatology by Christian et al. (2003) and other authors. Thus, a mean maximum value of about 157 fl km-2 y-1 is found for the annual lightning density. The zonal distribution of the lightning flashes exhibits a maximum between 1°S and 2°S and about 56 % of the flashes located below the equator in the 10°S - 10°N interval. The diurnal evolution of the flash rate has a maximum between 1400 and 1700 UTC, according to the reference year, in agreement with previous works in other regions of the world.

  11. Detailed genetic structure of European bitterling populations in Central Europe

    Directory of Open Access Journals (Sweden)

    Veronika Bartáková

    2015-11-01

    Full Text Available The European bitterling (Rhodeus amarus is a small cyprinid fish whose populations declined markedly between 1950 and 1980. However, its range currently expands, partly due to human-assisted introductions. We determined the genetic variability and detailed spatial structure among bitterling populations in Central Europe and tested alternative hypotheses about colonization of this area. Twelve polymorphic microsatellite loci on a large sample of 688 individuals had been used to analyse genetic variability and population structure. Samples originated from 27 localities with emphasis on area of the Czech Republic where three major sea drainages (Black, Baltic, and Northern Sea meet. Highly variable level of intrapopulation genetic variability had generally been detected and a recent decrease in numbers (“bottleneck” had been indicated by genetic data among six populations. High level of interpopulation differentiation was identified even within the basins. There was a significant role of genetic drift and indications of low dispersal ability of R. amarus. Surprisingly, the Odra River was inhabited by two distinct populations without any genetic signatures of a secondary contact. Czech part of the Odra (Baltic basin was colonized from the Danubian refugium (similarly to adjacent Danubian basin rivers including the Morava, while Polish part of the Odra was genetically similar to the populations in the Vistula River (Baltic basin, that has been colonized by a different (Eastern phylogeographic lineage of R. amarus. Most Czech R. amarus populations were colonized from the Danubian refugium, suggesting potential for a human-mediated colonization of the Odra or Elbe Rivers by R. amarus. One Elbe basin population was genetically mixed from the two (Danubian and Eastern phylogeographic lineages. In general the Czech populations of R. amarus were genetically stable except for a single population which has probably been recently introduced. This research

  12. Cellular Phone Towers, Cell towers developed for Appraiser's Department in 2003. Location was based upon parcel centroids, and corrected to orthophotography. Probably includes towers other than cell towers (uncertain). Not published., Published in 2003, 1:1200 (1in=100ft) scale, Sedgwick County, Kansas.

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — This Cellular Phone Towers dataset, published at 1:1200 (1in=100ft) scale, was produced all or in part from Other information as of 2003. It is described as 'Cell...

  13. Transport van perkplanten kan in details nog beter

    NARCIS (Netherlands)

    Harkema, H.; Westra, E.H.

    2012-01-01

    De perkgoedsector pakt veel zaken die de kwaliteit betreffen goed aan. Er zijn alleen nog wat details die beter kunnen. Te denken valt aan licht geven tijdens tranport en lage luchtvochtigheid voorkomen.

  14. CDC WONDER: Detailed Mortality - Underlying Cause of Death

    Data.gov (United States)

    U.S. Department of Health & Human Services — The Detailed Mortality - Underlying Cause of Death data on CDC WONDER are county-level national mortality and population data spanning the years 1999-2009. Data are...

  15. All the Exquisite Details of a Coffee Mug.

    Science.gov (United States)

    Larsen, Dave M., Jr.

    1999-01-01

    Describes a three-part exercise used in a first semester freshman composition class, intended to show students the world of details in even the most ordinary, everyday objects by having students write about a plastic coffee mug. (SR)

  16. A New Video Coding Method Based on Improving Detail Regions

    Institute of Scientific and Technical Information of China (English)

    2002-01-01

    The Moving Pictures Expert Group (MPEG) and H.263 standard coding method is widely used in video compression. However, the visual quality of detail regions such as eyes and mouth is not content in people at the decoder, as far as the conference telephone or videophone is concerned. A new coding method based on improving detail regions is presented in this paper. Experimental results show that this method can improve the visual quality at the decoder.

  17. Real-Time Crowdsourcing of Detailed Soccer Data

    OpenAIRE

    Perin, Charles; Vuillemot, Romain; Fekete, Jean-Daniel

    2013-01-01

    International audience This article explores how spectators of a live soccer game can collect detailed data while watching the game. Our motivation arouse from the lack of free detailed sport data, contrasting with the large amount of simple statistics collected for every popular games and available on the web. Assuming many spectators carry a smart phone during a game, we implemented a series of input interfaces for collecting data in real time. In a user study, we asked participants to u...

  18. Detail Enhancement for Infrared Images Based on Propagated Image Filter

    Directory of Open Access Journals (Sweden)

    Yishu Peng

    2016-01-01

    Full Text Available For displaying high-dynamic-range images acquired by thermal camera systems, 14-bit raw infrared data should map into 8-bit gray values. This paper presents a new method for detail enhancement of infrared images to display the image with a relatively satisfied contrast and brightness, rich detail information, and no artifacts caused by the image processing. We first adopt a propagated image filter to smooth the input image and separate the image into the base layer and the detail layer. Then, we refine the base layer by using modified histogram projection for compressing. Meanwhile, the adaptive weights derived from the layer decomposition processing are used as the strict gain control for the detail layer. The final display result is obtained by recombining the two modified layers. Experimental results on both cooled and uncooled infrared data verify that the proposed method outperforms the method based on log-power histogram modification and bilateral filter-based detail enhancement in both detail enhancement and visual effect.

  19. Sellafield site (including Drigg) emergency scheme manual

    International Nuclear Information System (INIS)

    This Emergency Scheme defines the organisation and procedures available should there be an accident at the Sellafield Site which results in, or may result in, the release of radioactive material, or the generation of a high radiation field, which might present a hazard to employees and/or the general public. This manual covers the general principles of the total emergency scheme and those detailed procedures which are not specific to any single department. (U.K.)

  20. Older adults report moderately more detailed autobiographical memories

    Directory of Open Access Journals (Sweden)

    Robert S Gardner

    2015-05-01

    Full Text Available Autobiographical memory (AM is an essential component of the human mind. Although the amount and types of subjective detail (content that compose AMs constitute important dimensions of recall, age-related changes in memory content are not well characterized. Previously, we introduced the Cue-Recalled Autobiographical Memory test (CRAM; see http://cramtest.info, an instrument that collects subjective reports of AM content, and applied it to college-aged subjects. CRAM elicits AMs using naturalistic word-cues. Subsequently, subjects date each cued AM to a life period and count the number of remembered details from specified categories (features, e.g., temporal detail, spatial detail, persons, objects, and emotions. The current work applies CRAM to a broad range of individuals (18-78 years old to quantify the effects of age on AM content. Subject age showed a moderately positive effect on AM content: older compared with younger adults reported ~16% more details (~25 vs. ~21 in typical AMs. This age-related increase in memory content was similarly observed for remote and recent AMs, although content declined with the age of the event among all subjects. In general, the distribution of details across features was largely consistent among younger and older adults. However, certain types of details, i.e., those related to objects and sequences of events, contributed more to the age effect on content. Altogether, this work identifies a moderate age-related feature-specific alteration in the way life events are subjectively recalled, among an otherwise stable retrieval profile.

  1. Older adults report moderately more detailed autobiographical memories.

    Science.gov (United States)

    Gardner, Robert S; Mainetti, Matteo; Ascoli, Giorgio A

    2015-01-01

    Autobiographical memory (AM) is an essential component of the human mind. Although the([A-z]+) amount and types of subjective detail (content) that compose AMs constitute important dimensions of recall, age-related changes in memory content are not well characterized. Previously, we introduced the Cue-Recalled Autobiographical Memory test (CRAM; see http://cramtest.info), an instrument that collects subjective reports of AM content, and applied it to college-aged subjects. CRAM elicits AMs using naturalistic word-cues. Subsequently, subjects date each cued AM to a life period and count the number of remembered details from specified categories (features), e.g., temporal detail, spatial detail, persons, objects, and emotions. The current work applies CRAM to a broad range of individuals (18-78 years old) to quantify the effects of age on AM content. Subject age showed a moderately positive effect on AM content: older compared with younger adults reported ∼16% more details (∼25 vs. ∼21 in typical AMs). This age-related increase in memory content was similarly observed for remote and recent AMs, although content declined with the age of the event among all subjects. In general, the distribution of details across features was largely consistent among younger and older adults. However, certain types of details, i.e., those related to objects and sequences of events, contributed more to the age effect on content. Altogether, this work identifies a moderate age-related feature-specific alteration in the way life events are subjectively recalled, among an otherwise stable retrieval profile. PMID:26042064

  2. Details and justifications for the MAP concept specification for acceleration above 63 GeV

    Energy Technology Data Exchange (ETDEWEB)

    Berg, J. Scott [Brookhaven National Lab. (BNL), Upton, NY (United States). Collider-Accelerator Dept.

    2014-02-28

    The Muon Accelerator Program (MAP) requires a concept specification for each of the accelerator systems. The Muon accelerators will bring the beam energy from a total energy of 63 GeV to the maximum energy that will fit on the Fermilab site. Justifications and supporting references are included, providing more detail than will appear in the concept specification itself.

  3. Mechanical Model of Geometric Cell and Topological Algorithm for Cell Dynamics from Single-Cell to Formation of Monolayered Tissues with Pattern

    KAUST Repository

    Kachalo, Sëma

    2015-05-14

    Geometric and mechanical properties of individual cells and interactions among neighboring cells are the basis of formation of tissue patterns. Understanding the complex interplay of cells is essential for gaining insight into embryogenesis, tissue development, and other emerging behavior. Here we describe a cell model and an efficient geometric algorithm for studying the dynamic process of tissue formation in 2D (e.g. epithelial tissues). Our approach improves upon previous methods by incorporating properties of individual cells as well as detailed description of the dynamic growth process, with all topological changes accounted for. Cell size, shape, and division plane orientation are modeled realistically. In addition, cell birth, cell growth, cell shrinkage, cell death, cell division, cell collision, and cell rearrangements are now fully accounted for. Different models of cell-cell interactions, such as lateral inhibition during the process of growth, can be studied in detail. Cellular pattern formation for monolayered tissues from arbitrary initial conditions, including that of a single cell, can also be studied in detail. Computational efficiency is achieved through the employment of a special data structure that ensures access to neighboring cells in constant time, without additional space requirement. We have successfully generated tissues consisting of more than 20,000 cells starting from 2 cells within 1 hour. We show that our model can be used to study embryogenesis, tissue fusion, and cell apoptosis. We give detailed study of the classical developmental process of bristle formation on the epidermis of D. melanogaster and the fundamental problem of homeostatic size control in epithelial tissues. Simulation results reveal significant roles of solubility of secreted factors in both the bristle formation and the homeostatic control of tissue size. Our method can be used to study broad problems in monolayered tissue formation. Our software is publicly

  4. Solar cell radiation handbook

    Science.gov (United States)

    Tada, H. Y.; Carter, J. R., Jr.; Anspaugh, B. E.; Downing, R. G.

    1982-01-01

    The handbook to predict the degradation of solar cell electrical performance in any given space radiation environment is presented. Solar cell theory, cell manufacturing and how they are modeled mathematically are described. The interaction of energetic charged particles radiation with solar cells is discussed and the concept of 1 MeV equivalent electron fluence is introduced. The space radiation environment is described and methods of calculating equivalent fluences for the space environment are developed. A computer program was written to perform the equivalent fluence calculations and a FORTRAN listing of the program is included. Data detailing the degradation of solar cell electrical parameters as a function of 1 MeV electron fluence are presented.

  5. A pilot study evaluating alternative approaches of academic detailing in rural family practice clinics

    Directory of Open Access Journals (Sweden)

    Hartung Daniel M

    2012-12-01

    Full Text Available Abstract Background Academic detailing is an interactive, convenient, and user-friendly approach to delivering non-commercial education to healthcare clinicians. While evidence suggests academic detailing is associated with improvements in prescribing behavior, uncertainty exists about generalizability and scalability in diverse settings. Our study evaluates different models of delivering academic detailing in a rural family medicine setting. Methods We conducted a pilot project to assess the feasibility, effectiveness, and satisfaction with academic detailing delivered face-to-face as compared to a modified approach using distance-learning technology. The recipients were four family medicine clinics within the Oregon Rural Practice-based Research Network (ORPRN. Two clinics were allocated to receive face-to-face detailing and two received outreach through video conferencing or asynchronous web-based outreach. Surveys at midpoint and completion were used to assess effectiveness and satisfaction. Results Each clinic received four outreach visits over an eight month period. Topics included treatment-resistant depression, management of atypical antipsychotics, drugs for insomnia, and benzodiazepine tapering. Overall, 90% of participating clinicians were satisfied with the program. Respondents who received in person detailing reported a higher likelihood of changing their behavior compared to respondents in the distance detailing group for five of seven content areas. While 90%-100% of respondents indicated they would continue to participate if the program were continued, the likelihood of participation declined if only distance approaches were offered. Conclusions We found strong support and satisfaction for the program among participating clinicians. Participants favored in-person approaches to distance interactions. Future efforts will be directed at quantitative methods for evaluating the economic and clinical effectiveness of detailing in rural

  6. State of the States. Fuel Cells in America 2012

    Energy Technology Data Exchange (ETDEWEB)

    Curtin, Sandra [Fuel Cells 2000, Washington, DC (United States); Gangi, Jennifer [Fuel Cells 2000, Washington, DC (United States); Skukowski, Ryan [Fuel Cells 2000, Washington, DC (United States)

    2012-09-01

    This report, written by Fuel Cells 2000 and partially funded by the U.S. Department of Energy's Fuel Cell Technologies Program, continues to build on the April 2010 State of the States report that provided a snapshot of fuel cell and hydrogen activity in the 50 states and District of Columbia. This update report provides more details on the progress and activities that happened since the second report, issued in June 2011. Details reported for each state include new policies and funding, recent and planned fuel cell and hydrogen installations, and recent activity by state industry and universities.

  7. Steel-framed buildings: Impacts of wall detail configurations on the whole wall thermal performance

    Energy Technology Data Exchange (ETDEWEB)

    Kosny, J.; Desjarlais, A.O.; Christian, J.E.

    1998-06-01

    The main objective of this paper is the influence of architectural wall details on the whole wall thermal performance. Whole wall thermal performance analysis was performed for six light gage steel-framed wall systems (some with wood components). For each wall system, all wall details were simulated using calibrated 3-D finite difference computer modeling. The thermal performance of the six steel-framed wall systems included various system details and the whole wall system thermal performance for a typical single-story ranch house. Currently, predicted heat losses through building walls are typically based on measurements of the wall system clear wall area using test methods such as ASTM C 236 or are calculated by one of the procedures recommended in the ASHRAE Handbook of Fundamentals that often is carried out for the clear wall area exclusively. In this paper, clear wall area is defined as the part of the wall system that is free of thermal anomalies due to building envelope details or thermally unaffected by intersections with other surfaces of the building envelope. Clear wall experiments or calculations normally do not include the effects of building envelope details such as corners, window and door openings, and structural intersections with roofs, floors, ceilings, and other walls. In steel-framed wall systems, these details typically consist of much more structural components than the clear wall. For this situation, the thermal properties measured or calculated for the clear wall area do not adequately represent the total wall system thermal performance. Factors that would impact the ability of today`s standard practice to accurately predict the total wall system thermal performance are the accuracy of the calculation methods, the area of the total wall that is clear wall, and the quantity and thermal performance of the various wall system details.

  8. Selectively detail-enhanced fusion of differently exposed images with moving objects.

    Science.gov (United States)

    Li, Zhengguo; Zheng, Jinghong; Zhu, Zijian; Wu, Shiqian

    2014-10-01

    In this paper, we introduce an exposure fusion scheme for differently exposed images with moving objects. The proposed scheme comprises a ghost removal algorithm in a low dynamic range domain and a selectively detail-enhanced exposure fusion algorithm. The proposed ghost removal algorithm includes a bidirectional normalization-based method for the detection of nonconsistent pixels and a two-round hybrid method for the correction of nonconsistent pixels. Our detail-enhanced exposure fusion algorithm includes a content adaptive bilateral filter, which extracts fine details from all the corrected images simultaneously in gradient domain. The final image is synthesized by selectively adding the extracted fine details to an intermediate image that is generated by fusing all the corrected images via an existing multiscale algorithm. The proposed exposure fusion algorithm allows fine details to be exaggerated while existing exposure fusion algorithms do not provide such an option. The proposed scheme usually outperforms existing exposure fusion schemes when there are moving objects in real scenes. In addition, the proposed ghost removal algorithm is simpler than existing ghost removal algorithms and is suitable for mobile devices with limited computational resource. PMID:25148667

  9. Testing for detailed balance in a financial market

    Science.gov (United States)

    Fiebig, H. R.; Musgrove, D. P.

    2015-06-01

    We test a historical price-time series in a financial market (the NASDAQ 100 index) for a statistical property known as detailed balance. The presence of detailed balance would imply that the market can be modeled by a stochastic process based on a Markov chain, thus leading to equilibrium. In economic terms, a positive outcome of the test would support the efficient market hypothesis, a cornerstone of neo-classical economic theory. In contrast to the usage in prevalent economic theory the term equilibrium here is tied to the returns, rather than the price-time series. The test is based on an action functional S constructed from the elements of the detailed balance condition and the historical data set, and then analyzing S by means of simulated annealing. Checks are performed to verify the validity of the analysis method. We discuss the outcome of this analysis.

  10. Study on Hierarchical Structure of Detailed Control Planning

    Institute of Scientific and Technical Information of China (English)

    2010-01-01

    Using case studies,this paper analyzes the characteristics of detailed control planning and its hierarchical controls,the form and composition of plan content,and methodological innovations.It then suggests improvements to the planning structure that are oriented at adaptability,fairness,centrality,and scientific principles with regard to the content,methods,and results of the planning.Regarding the hierarchical control system,the paper suggests that the detailed control plan should be composed of "block planning" and "plot planning".It is believed that through a combination of block and plot planning,the problem of joining long-term and short-term planning will be solved and it will be possible to address the need for adjustment and revision of detailed control plan.

  11. Probabilistic Model for Fatigue Crack Growth in Welded Bridge Details

    DEFF Research Database (Denmark)

    Toft, Henrik Stensgaard; Sørensen, John Dalsgaard; Yalamas, Thierry;

    2013-01-01

    In the present paper a probabilistic model for fatigue crack growth in welded steel details in road bridges is presented. The probabilistic model takes the influence of bending stresses in the joints into account. The bending stresses can either be introduced by e.g. misalignment or redistribution...... of stresses in the structure. The fatigue stress ranges are estimated from traffic measurements and a generic bridge model. Based on the probabilistic models for the resistance and load the reliability is estimated for a typical welded steel detail. The results show that large misalignments in the...

  12. Tile-based Level of Detail for the Parallel Age

    Energy Technology Data Exchange (ETDEWEB)

    Niski, K; Cohen, J D

    2007-08-15

    Today's PCs incorporate multiple CPUs and GPUs and are easily arranged in clusters for high-performance, interactive graphics. We present an approach based on hierarchical, screen-space tiles to parallelizing rendering with level of detail. Adapt tiles, render tiles, and machine tiles are associated with CPUs, GPUs, and PCs, respectively, to efficiently parallelize the workload with good resource utilization. Adaptive tile sizes provide load balancing while our level of detail system allows total and independent management of the load on CPUs and GPUs. We demonstrate our approach on parallel configurations consisting of both single PCs and a cluster of PCs.

  13. Article Including Environmental Barrier Coating System

    Science.gov (United States)

    Lee, Kang N. (Inventor)

    2015-01-01

    An enhanced environmental barrier coating for a silicon containing substrate. The enhanced barrier coating may include a bond coat doped with at least one of an alkali metal oxide and an alkali earth metal oxide. The enhanced barrier coating may include a composite mullite bond coat including BSAS and another distinct second phase oxide applied over said surface.

  14. Static, Lightweight Includes Resolution for PHP

    NARCIS (Netherlands)

    Hills, M.A.; Klint, P.; Vinju, J.J.

    2014-01-01

    Dynamic languages include a number of features that are challenging to model properly in static analysis tools. In PHP, one of these features is the include expression, where an arbitrary expression provides the path of the file to include at runtime. In this paper we present two complementary analy

  15. European Fuel Cells R&D Review

    Science.gov (United States)

    Michael, P. D.; Maguire, J.

    1994-09-01

    A review is presented on the status of fuel cell development in Europe, addressing the research, development, and demonstration (RD&D) and commercialization activities being undertaken, identifying key European organizations active in development and commercialization of fuel cells, and detailing their future plans. This document describes the RD&D activities in Europe on alkaline, phosphoric acid, polymer electrolyte, direct methanol, solid oxide, and molten carbonate fuel cell types. It describes the European Commission's activities, its role in the European development of fuel cells, and its interaction with the national programs. It then presents a country-by-country breakdown. For each country, an overview is given, presented by fuel cell type. Scandinavian countries are covered in less detail. American organizations active in Europe, either in supplying fuel cell components, or in collaboration, are identified. Applications include transportation and cogeneration.

  16. Pharmaceutical crystallography: is there a devil in the details?

    DEFF Research Database (Denmark)

    Bond, A. D.

    2012-01-01

    Modern instruments for small-molecule crystallography continue to become more sophisticated and more automated. This technical progress provides a basis for frontier research in chemical and pharmaceutical crystallography, but it also encourages analytical crystallographers to become more...... are presented for pharmaceutical compounds, and the potential importance of the "details" in pharmaceutical crystallography is discussed....

  17. Details from the Dashboard: Charter School Race/Ethnicity Demographics

    Science.gov (United States)

    National Alliance for Public Charter Schools, 2012

    2012-01-01

    This "Details from the Dashboard" report examines race/ethnicity breakouts for public charter schools and traditional public schools at the state and the school district level. The data in this report indicate that in the large majority of states, the race/ethnicity student demographics of charter schools are almost identical to those of the…

  18. Detailed behavioral modeling of bang-bang phase detectors

    DEFF Research Database (Denmark)

    Jiang, Chenhui; Andreani, Pietro; Keil, U. D.

    2006-01-01

    In this paper, the metastability of current-mode logic (CML) latches and flip-flops is studied in detail. Based on the results of this analysis, a behavioral model of bang-bang phase detectors (BBPDs) is proposed, which is able to reliably capture the critical deadzone effect. The impact of jitter...

  19. Energetic Mapping of Ni Catalysts by Detailed Kinetic Modeling

    DEFF Research Database (Denmark)

    Bjørgum, Erlend; Chen, De; Bakken, Mari G.;

    2005-01-01

    precursor seems to result in more steplike sites, kinks, and defects for carbon monoxide dissociation. A detailed kinetic modeling of the TPO results based on elementary reaction steps has been conducted to give an energetic map of supported Ni catalysts. Experimental results from the ideal Ni surface fit...

  20. Complex and detailed balancing of chemical reaction networks revisited

    NARCIS (Netherlands)

    van der Schaft, Abraham; Rao, Shodhan; Jayawardhana, Bayu

    2015-01-01

    The characterization of the notions of complex and detailed balancing for mass action kinetics chemical reaction networks is revisited from the perspective of algebraic graph theory, in particular Kirchhoff’s Matrix Tree theorem for directed weighted graphs. This yields an elucidation of previously

  1. A Detailed Survey on Various Tracking Methods Using RFID

    OpenAIRE

    S. Arivarasi; S. Krishna Anand

    2013-01-01

    RFID, a wide range of technology is replaced instead of barcode. When multiple RFID readers find a single RFID tag at same time, duplicates occur. The main objective is to eliminate duplicates in RFID data. Designing an exact duplicate elimination method is very difficult. This paper presented a detailed analysis of tracking, monitoring, managing and filtering mechanisms for RFID data.

  2. Details from the Dashboard: Charter Schools by Geographic Region

    Science.gov (United States)

    National Alliance for Public Charter Schools, 2012

    2012-01-01

    While a majority of charter schools nationwide operate in urban and suburban areas, charter schools exist in all corners of the nation, and are expanding into all types of communities. This "Details from the Dashboard" report presents statistics on the number of charter schools and students enrolled in charter schools by the four geographic…

  3. Detailed bathymetric surveys in the central Indian Basin

    Digital Repository Service at National Institute of Oceanography (India)

    Kodagali, V.N.; KameshRaju, K.A.; Ramprasad, T.; George, P.; Jaisankar, S.

    Over 420,000 line kilometers of echo-sounding data was collected in the Central Indian Basin. This data was digitized, merged with navigation data and a detailed bathymetric map of the Basin was prepared. The Basin can be broadly classified...

  4. Detailed analysis of harmonic effects in the static Kramer drive

    Energy Technology Data Exchange (ETDEWEB)

    Zahawi, B.A. [Manchester School of Engineering, University of Manchester (United Kingdom); Refoufi, L. [Faculty of Engineering, Universite de Boumerdes (Algeria)

    2000-08-01

    A comprehensive examination of harmonic effect in slipenergy recovery drives employing a diode bridge rectifier in the rotor recovery circuit is presented. Torque and stator current pulsations at several speeds of operation are investigated in detail. Experimental verification of computed quantities shows excellent agreement. (orig.)

  5. 20 CFR 422.604 - Request for detailed information.

    Science.gov (United States)

    2010-04-01

    ... Section 422.604 Employees' Benefits SOCIAL SECURITY ADMINISTRATION ORGANIZATION AND PROCEDURES Administrative Review Process Under the Coal Industry Retiree Health Benefit Act of 1992 § 422.604 Request for... whom you have premium responsibility, you may request detailed information as to the work histories...

  6. Pipeline and energy facility siting: getting at the relevant details

    Energy Technology Data Exchange (ETDEWEB)

    Desautels, Denise L.; Mayberry, Alan K.

    2007-10-15

    Due process requirements may be time-consuming and expensive, but they result in an agency being provided the information it needs to make a decision even as the affected participants are given an adequate voice. Here's some advice on how to insure that the crucial details come to the forefront. (author)

  7. Video-based facial animation with detailed appearance texture

    Institute of Scientific and Technical Information of China (English)

    2006-01-01

    Facial shape transformation described by facial animation parameters (FAPs) involves the dynamic movement or deformation of eyes, brows, mouth, and lips, while detailed facial appearance concerns the facial textures such as creases, wrinkles, etc.Video-based facial animation exhibits not only facial shape transformation but also detailed appearance updates. In this paper, a novel algorithm for effectively extracting FAPs from video is proposed. Our system adopts the ICA-enforced direct appearance model (DAM) to track faces from video sequences; and then, FAPs are extracted from every frame of the video based on an extended model of Wincandidate 3.1. Facial appearance details are transformed from each frame by mapping an expression ratio image to the original image. We adopt wavelet to synthesize expressive details by combining the low-frequency signals of the original face and high-frequency signals of the expressive face from each frame of the video. Experimental results show that our proposed algorithm is suitable for reproducing realistic, expressive facial animations.

  8. A detailed breast model and its application for Chinese female breast dose estimation in mammography

    International Nuclear Information System (INIS)

    The IAEA protocol for breast dosimetry is based on the calculations of Dance which used very simple models of the breast based on the western female data. In order to estimate the dose more precisely in the mammographic examination for the Chinese female, a 3D detailed breast model was constructed. This model contains the detailed structures in the breast, including skin, subcutaneous fat, Cooper's ligaments, and fibro-glandular region containing intra-glandular fat, gland, ductal system, and terminal ductal lobular units. And the size of this breast model was adjusted to represent the characteristic of Chinese female. The glandular tissue conversion coefficients were calculated using this detailed breast model with Monte Carlo code, Geant4. The comparison between the data obtained in this study and the data from Dance was carried out. The discrepancy may come from both the structure and size of the breast models

  9. Analysis of Dynamic Interactions between Different Drivetrain Components with a Detailed Wind Turbine Model

    Science.gov (United States)

    Bartschat, A.; Morisse, M.; Mertens, A.; Wenske, J.

    2016-09-01

    The presented work describes a detailed analysis of the dynamic interactions among mechanical and electrical drivetrain components of a modern wind turbine under the influence of parameter variations, different control mechanisms and transient excitations. For this study, a detailed model of a 2MW wind turbine with a gearbox, a permanent magnet synchronous generator and a full power converter has been developed which considers all relevant characteristics of the mechanical and electrical subsystems. This model includes an accurate representation of the aerodynamics and the mechanical properties of the rotor and the complete mechanical drivetrain. Furthermore, a detailed electrical modelling of the generator, the full scale power converter with discrete switching devices, its filters, the transformer and the grid as well as the control structure is considered. The analysis shows that, considering control measures based on active torsional damping, interactions between mechanical and electrical subsystems can significantly affect the loads and thus the individual lifetime of the components.

  10. Composite Pressure Vessel Including Crack Arresting Barrier

    Science.gov (United States)

    DeLay, Thomas K. (Inventor)

    2013-01-01

    A pressure vessel includes a ported fitting having an annular flange formed on an end thereof and a tank that envelopes the annular flange. A crack arresting barrier is bonded to and forming a lining of the tank within the outer surface thereof. The crack arresting barrier includes a cured resin having a post-curing ductility rating of at least approximately 60% through the cured resin, and further includes randomly-oriented fibers positioned in and throughout the cured resin.

  11. Fuel Cell Handbook, Fourth Edition

    Energy Technology Data Exchange (ETDEWEB)

    Stauffer, D.B; Hirschenhofer, J.H.; Klett, M.G.; Engleman, R.R.

    1998-11-01

    Robust progress has been made in fuel cell technology since the previous edition of the Fuel Cell Handbook was published in January 1994. This Handbook provides a foundation in fuel cells for persons wanting a better understanding of the technology, its benefits, and the systems issues that influence its application. Trends in technology are discussed, including next-generation concepts that promise ultra high efficiency and low cost, while providing exceptionally clean power plant systems. Section 1 summarizes fuel cell progress since the last edition and includes existing power plant nameplate data. Section 2 addresses the thermodynamics of fuel cells to provide an understanding of fuel cell operation at two levels (basic and advanced). Sections 3 through 6 describe the four major fuel cell types and their performance based on cell operating conditions. The section on polymer electrolyte membrane fuel cells has been added to reflect their emergence as a significant fuel cell technology. Phosphoric acid, molten carbonate, and solid oxide fuel cell technology description sections have been updated from the previous edition. New information indicates that manufacturers have stayed with proven cell designs, focusing instead on advancing the system surrounding the fuel cell to lower life cycle costs. Section 7, Fuel Cell Systems, has been significantly revised to characterize near-term and next-generation fuel cell power plant systems at a conceptual level of detail. Section 8 provides examples of practical fuel cell system calculations. A list of fuel cell URLs is included in the Appendix. A new index assists the reader in locating specific information quickly.

  12. Talent in autism: hyper-systemizing, hyper-attention to detail and sensory hypersensitivity

    Science.gov (United States)

    Baron-Cohen, Simon; Ashwin, Emma; Ashwin, Chris; Tavassoli, Teresa; Chakrabarti, Bhismadev

    2009-01-01

    We argue that hyper-systemizing predisposes individuals to show talent, and review evidence that hyper-systemizing is part of the cognitive style of people with autism spectrum conditions (ASC). We then clarify the hyper-systemizing theory, contrasting it to the weak central coherence (WCC) and executive dysfunction (ED) theories. The ED theory has difficulty explaining the existence of talent in ASC. While both hyper-systemizing and WCC theories postulate excellent attention to detail, by itself excellent attention to detail will not produce talent. By contrast, the hyper-systemizing theory argues that the excellent attention to detail is directed towards detecting ‘if p, then q’ rules (or [input–operation–output] reasoning). Such law-based pattern recognition systems can produce talent in systemizable domains. Finally, we argue that the excellent attention to detail in ASC is itself a consequence of sensory hypersensitivity. We review an experiment from our laboratory demonstrating sensory hypersensitivity detection thresholds in vision. We conclude that the origins of the association between autism and talent begin at the sensory level, include excellent attention to detail and end with hyper-systemizing. PMID:19528020

  13. General aspects of immunotoxicology including validation issues

    NARCIS (Netherlands)

    Frieke Kuper, C.

    2006-01-01

    Histologic examination of lymphoid organs has revealed immunotoxic effects of a broad range of substances. The thymus has proven especially sensitive. The relative lack of sensitivity of mucosa-associated lymphoid cells and tissues may be due to shortcomings in the way they are examined. Validation

  14. Sellafield Site (including Drigg) emergency scheme manual

    International Nuclear Information System (INIS)

    This Scheme defines the organisation and procedures available should there be an accident at the Sellafield Site which results in, or may result in, the release of radioactive material, or the generation of a high radiation field, which might present a hazard to employees and/or the general public. Several categories of emergencies on the Sellafield Site are mentioned; a building emergency which is confined to one building, a Site emergency standby when the effects of a building emergency go outside that building, a Site emergency alert (District Emergency Standby) when a release of activity affects Site operations and could have serious Site effects and a District Emergency Alert when a radioactivity release may interfere with the normal activity of the General Public. A Drigg Emergency Standby situation would operate similarly at the Drigg Site. The detailed arrangements and responsibilities of appointed personnel are set out in this manual. (UK)

  15. 28 CFR 20.32 - Includable offenses.

    Science.gov (United States)

    2010-07-01

    ... 28 Judicial Administration 1 2010-07-01 2010-07-01 false Includable offenses. 20.32 Section 20.32 Judicial Administration DEPARTMENT OF JUSTICE CRIMINAL JUSTICE INFORMATION SYSTEMS Federal Systems and Exchange of Criminal History Record Information § 20.32 Includable offenses. (a) Criminal history...

  16. An Investigation of Placement and Type of Seductive Details: The Primacy Effect of Seductive Details on Text Recall

    Science.gov (United States)

    Rowland, Emily; Skinner, Christopher H.; Davis-Richards, Kai; Saudargas, Richard; Robinson, Daniel H.

    2008-01-01

    Seductive details are interesting, but sometimes irrelevant to the target material present in texts and lectures. In the current study, 388 undergraduate students read six paragraphs describing Sigmund Freud's psychosexual stages (i.e., target material). Participants in four groups also read one of two biographical paragraphs. The biographical…

  17. Detailed characterization of anode-supported SOFCs by impedance spectroscopy

    DEFF Research Database (Denmark)

    Barfod, R.; Mogensen, Mogens Bjerg; Klemensø, Trine;

    2007-01-01

    Anode-supported thin electrolyte cells are studied by electrochemical impedance spectroscopy (EIS). The aim is to describe how the losses of this type of cells are distributed at low current density (around open-circuit voltage) as a function of temperature. An equivalent circuit consisting...

  18. Results of Detailed Hydrologic Characterization Tests—Fiscal and Calendar Year 2005

    Energy Technology Data Exchange (ETDEWEB)

    Spane, Frank A.; Newcomer, Darrell R.

    2008-02-27

    This report provides the results of detailed hydrologic characterization tests conducted within selected Hanford Site wells during fiscal and calendar year 2005. Detailed characterization tests performed included groundwater-flow characterization, barometric response evaluation, slug tests, in-well vertical groundwater-flow assessments, and a single-well tracer and constant-rate pumping test. Hydraulic property estimates obtained from the detailed hydrologic tests include hydraulic conductivity, transmissivity, specific yield, effective porosity, in-well lateral and vertical groundwater-flow velocity, aquifer groundwater-flow velocity, and depth-distribution profiles of hydraulic conductivity. In addition, local groundwater-flow characteristics (i.e., hydraulic gradient and flow direction) were determined for a site where detailed well testing was performed. Results obtained from these tests provide hydrologic information that supports the needs of Resource Conservation and Recovery Act waste management area characterization as well as sitewide groundwater monitoring and modeling programs. These results also reduce the uncertainty of groundwater-flow conditions at selected locations on the Hanford Site.

  19. Detailed kinetic modeling of the thermal degradation of lignins

    International Nuclear Information System (INIS)

    The aim of this kinetic work is to provide a better understanding of the pyrolysis of lignin and biomasses not only in terms of devolatilazation rate but also of the volatile species released. The complexity of both lignin structure and its degradation mechanism meant that a lumping approach suitable for handling the huge amount of initial, intermediate and final products had to be used. Despite these simplifications, the proposed semi-detailed kinetic scheme involves about 100 molecular and radical species in 500 elementary and lumped reactions. It has already been proved that this lignin devolatilization model correctly predicts the degradation rates and the detail of the released products. This work constitutes an initial yet significant step towards deriving a complete kinetic scheme of biomass devolatilization.

  20. Samnett: the EMPS model with power flow constraints: implementation details

    Energy Technology Data Exchange (ETDEWEB)

    Helseth, Arild; Warland, Geir; Mo, Birger; Fosso, Olav B.

    2011-12-15

    This report describes the development and implementation of Samnett. Samnett is a new prototype for solving the coupled market and transmission network problem. The prototype is based on the EMPS model (Samkjoeringsmodellen). Results from the market model are distributed to a detailed transmission network model, where a DC power flow detects if there are overloads on monitored lines or interconnections. In case of overloads, power flow constraints are generated and added to the market problem. This report is an updated version of TR A6891 {sup I}mplementing Network Constraints in the EMPS model{sup .} It further elaborates on theoretical and implementation details in Samnett, but does not contain the case studies and file descriptions presented in TR A6891.(auth)

  1. DETAILED MODELLING OF CHARGING BEHAVIOUR OF SMART SOLAR TANKS

    DEFF Research Database (Denmark)

    Fan, Jianhua; Andersen, Elsa; Furbo, Simon;

    The charging behaviour of smart solar tanks for solar combisystems for one-family houses is investigated with detailed Computational Fluid Dynamics (CFD) modelling and Particle Image Velocimetry (PIV) measurements. The smart solar tank can be charged with a variable auxiliary volume fitted...... to the expected future energy demand. Therefore the heat loss from the tank is decreased and the thermal performance of the solar heating system is increased compared to a traditional system with a fixed auxiliary volume. The solar tank can be charged either by an electric heating element situated in the tank...... or by an electric heating element in a side-arm mounted on the side of the tank. Detailed CFD models of the smart tanks are built with different mesh densities in the tank and in the side-arm. The thermal conditions of the tank during charging are calculated with the CFD models. The fluid flow and temperature...

  2. DETAILED MODELLING OF CHARGING BEHAVIOUR OF SMART SOLAR TANKS

    DEFF Research Database (Denmark)

    Fan, Jianhua; Andersen, Elsa; Furbo, Simon;

    2010-01-01

    The charging behaviour of smart solar tanks for solar combisystems for one-family houses is investigated with detailed Computational Fluid Dynamics (CFD) modelling and Particle Image Velocimetry (PIV) measurements. The smart solar tank can be charged with a variable auxiliary volume fitted...... to the expected future energy demand. Therefore the heat loss from the tank is decreased and the thermal performance of the solar heating system is increased compared to a traditional system with a fixed auxiliary volume. The solar tank can be charged either by an electric heating element situated in the tank...... or by an electric heating element in a side-arm mounted on the side of the tank. Detailed CFD models of the smart tanks are built with different mesh densities in the tank and in the side-arm. The thermal conditions of the tank during charging are calculated with the CFD models. The fluid flow and temperature...

  3. Exploring Architectural Details Through a Wearable Egocentric Vision Device.

    Science.gov (United States)

    Alletto, Stefano; Abati, Davide; Serra, Giuseppe; Cucchiara, Rita

    2016-01-01

    Augmented user experiences in the cultural heritage domain are in increasing demand by the new digital native tourists of 21st century. In this paper, we propose a novel solution that aims at assisting the visitor during an outdoor tour of a cultural site using the unique first person perspective of wearable cameras. In particular, the approach exploits computer vision techniques to retrieve the details by proposing a robust descriptor based on the covariance of local features. Using a lightweight wearable board, the solution can localize the user with respect to the 3D point cloud of the historical landmark and provide him with information about the details at which he is currently looking. Experimental results validate the method both in terms of accuracy and computational effort. Furthermore, user evaluation based on real-world experiments shows that the proposal is deemed effective in enriching a cultural experience. PMID:26901197

  4. Exploring Architectural Details Through a Wearable Egocentric Vision Device

    Directory of Open Access Journals (Sweden)

    Stefano Alletto

    2016-02-01

    Full Text Available Augmented user experiences in the cultural heritage domain are in increasing demand by the new digital native tourists of 21st century. In this paper, we propose a novel solution that aims at assisting the visitor during an outdoor tour of a cultural site using the unique first person perspective of wearable cameras. In particular, the approach exploits computer vision techniques to retrieve the details by proposing a robust descriptor based on the covariance of local features. Using a lightweight wearable board, the solution can localize the user with respect to the 3D point cloud of the historical landmark and provide him with information about the details at which he is currently looking. Experimental results validate the method both in terms of accuracy and computational effort. Furthermore, user evaluation based on real-world experiments shows that the proposal is deemed effective in enriching a cultural experience.

  5. Neutrino diffusive transport in hot quark matter: a detailed analysis

    CERN Document Server

    Colvero, Gustavo C

    2014-01-01

    We perform an extensive analysis of neutrino diffusion in quark matter within the MIT bag model at arbitrary temperature and degeneracy. We examine in detail the contribution of each of the relevant weak interaction processes to the total neutrino opacity and evaluate the effect of the strange quark mass, the bag constant, and the QCD perturbative corrections to the MIT bag model. We also investigate the anisotropic contribution to the neutrino distribution function in scatterings, the mean energy transfer and the mean scattering angle. The density and temperature dependence of the diffusion coefficients $D_n$ that govern the cooling and deleptonization of a compact star is shown in detail. Finally, our numerical results for the neutrino mean free paths are compared against known analytic approximations. We conclude that neutrino scattering constitutes a significant portion of the total neutrino opacity in leptonized quark matter and neutrino-quark scattering is, in general, very similar to neutrino-electron ...

  6. Detailed assessment of homology detection using different substitution matrices

    Institute of Scientific and Technical Information of China (English)

    LI Jing; WANG Wei

    2006-01-01

    Homology detection plays a key role in bioinformatics, whereas substitution matrix is one of the most important components in homology detection. Thus, besides the improvement of alignment algorithms, another effective way to enhance the accuracy of homology detection is to use proper substitution matrices or even construct new matrices.A study on the features of various matrices and on the comparison of the performances between different matrices in homology detection enable us to choose the most proper or optimal matrix for some specific applications. In this paper, by taking BLOSUM matrices as an example, some detailed features of matrices in homology detection are studied by calculating the distributions of numbers of recognized proteins over different sequence identities and sequence lengths. Our results clearly showed that different matrices have different preferences and abilities to the recognition of remote homologous proteins. Furthermore, detailed features of the various matrices can be used to improve the accuracy of homology detection.

  7. Detailed thermal-hydraulic computation into a containment building

    Energy Technology Data Exchange (ETDEWEB)

    Caruso. A.; Flour, I.; Simonin, O. [EDF/LNH, Chatou (France); Cherbonnel, C [EDF/SEPTEN, Villeurbanne (France)

    1995-09-01

    This paper deals with numerical predictions of the influence of water sprays upon stratifications into a containment building using a two-dimensional two-phase flow code. Basic equations and closure assumptions are briefly presented. A test case in a situation involving spray evaporation is first detailed to illustrate the validation step. Then results are presented for a compressible recirculating flow into a containment building with condensation phenomena.

  8. Influence of Cannabis on Fatal Traffic Crash: A Detailed Analysis

    OpenAIRE

    VAN ELSLANDE, Pierre; FOURNIER, Jean-Yves; Jaffard, Magali

    2012-01-01

    The influence of cannabis on traffic crashes is a growing concern. Experimental studies provide ample evidences of cannabis influence on psychomotor and cognitive performances. Epidemiological works describe the excess crash risk that this substance causes. And yet, this psychotropic drug influence in causing crashes is still at the centre of many discussions. The present analysis consists in exploiting crash data in detail to obtain a more precise understanding of the failures that drivers a...

  9. DETAILED MODELLING OF CHARGING BEHAVIOUR OF SMART SOLAR TANKS

    OpenAIRE

    Fan, Jianhua; Andersen, Elsa; Furbo, Simon; Perers, Bengt

    2010-01-01

    The charging behaviour of smart solar tanks for solar combisystems for one-family houses is investigated with detailed Computational Fluid Dynamics (CFD) modelling and Particle Image Velocimetry (PIV) measurements. The smart solar tank can be charged with a variable auxiliary volume fitted to the expected future energy demand. Therefore the heat loss from the tank is decreased and the thermal performance of the solar heating system is increased compared to a traditional system with a fixed au...

  10. Technology of Strengthening Steel Details by Surfacing Composite Coatings

    Science.gov (United States)

    Burov, V. G.; Bataev, A. A.; Rakhimyanov, Kh M.; Mul, D. O.

    2016-04-01

    The article considers the problem of forming wear resistant meal ceramic coatings on steel surfaces using the results of our own investigations and the analysis of achievements made in the country and abroad. Increasing the wear resistance of surface layers of steel details is achieved by surfacing composite coatings with carbides or borides of metals as disperse particles in the strengthening phase. The use of surfacing on wearing machine details and mechanisms has a history of more than 100 years. But still engineering investigations in this field are being conducted up to now. The use of heating sources which provide a high density of power allows ensuring temperature and time conditions of surfacing under which composites with peculiar service and functional properties are formed. High concentration of energy in the zone of melt, which is created from powder mixtures and the hardened surface layer, allows producing the transition zone between the main material and surfaced coating. Surfacing by the electron beam directed from vacuum to the atmosphere is of considerable technological advantages. They give the possibility of strengthening surface layers of large-sized details by surfacing powder mixtures without their preliminary compacting. A modified layer of the main metal with ceramic particles distributed in it is created as a result of heating surfaced powders and the detail surface layer by the electron beam. Technology of surfacing allows using powders of refractory metals and graphite in the composition of powder mixtures. They interact with one another and form the particles of the hardening phase of the composition coating. The chemical composition of the main and surfaced materials is considered to be the main factor which determines the character of metallurgical processes in local zones of melt as well as the structure and properties of surfaced composition.

  11. NUCLEAR ESPIONAGE: Report Details Spying on Touring Scientists.

    Science.gov (United States)

    Malakoff, D

    2000-06-30

    A congressional report released this week details dozens of sometimes clumsy attempts by foreign agents to obtain nuclear secrets from U.S. nuclear scientists traveling abroad, ranging from offering scientists prostitutes to prying off the backs of their laptop computers. The report highlights the need to better prepare traveling researchers to safeguard secrets and resist such temptations, say the two lawmakers who requested the report and officials at the Department of Energy, which employs the scientists. PMID:17769833

  12. "Academic detailing" improving prescribing practices in North Vancouver, conference told

    OpenAIRE

    Silversides, A.

    1997-01-01

    Physician prescribing practices were the focus of a recent 1-day conference in Toronto. A BC hospital pharmacist outlined a successful initiative that provides physicians with impartial prescribing advice, saying it has resulted in considerable savings and improved prescribing practices in North Vancouver. Drugs of Choice author Dr. Joel Lexchin says such initiatives, called academic detailing, along with peer feedback, are cost-effective ways to improve prescribing habits.

  13. Strategic Pricing and Detailing Behavior in International Markets

    OpenAIRE

    Pradeep K. Chintagunta; Ramarao Desiraju

    2005-01-01

    We study three determinants of the levels of price and detailing effort across geographic markets: the within-market response to each variable, the nature of interfirm strategic interactions both within that market and across markets. We empirically examine the interactions of Prozac, Zoloft, and Paxil across the United States, the United Kingdom, Germany, France, and Italy. Our results indicate that all three factors driving marketing mix interactions are at play in this product category. Th...

  14. Detail enhancement of blurred infrared images based on frequency extrapolation

    Science.gov (United States)

    Xu, Fuyuan; Zeng, Deguo; Zhang, Jun; Zheng, Ziyang; Wei, Fei; Wang, Tiedan

    2016-05-01

    A novel algorithm for enhancing the details of the blurred infrared images based on frequency extrapolation has been raised in this paper. Unlike other researchers' work, this algorithm mainly focuses on how to predict the higher frequency information based on the Laplacian pyramid separation of the blurred image. This algorithm uses the first level of the high frequency component of the pyramid of the blurred image to reverse-generate a higher, non-existing frequency component, and adds back to the histogram equalized input blurred image. A simple nonlinear operator is used to analyze the extracted first level high frequency component of the pyramid. Two critical parameters are participated in the calculation known as the clipping parameter C and the scaling parameter S. The detailed analysis of how these two parameters work during the procedure is figure demonstrated in this paper. The blurred image will become clear, and the detail will be enhanced due to the added higher frequency information. This algorithm has the advantages of computational simplicity and great performance, and it can definitely be deployed in the real-time industrial applications. We have done lots of experiments and gave illustrations of the algorithm's performance in this paper to convince its effectiveness.

  15. Applications of GPR in Structural Detailing of the Medway Tunnel

    Science.gov (United States)

    Alani, Amir M.; Faramarzi, Assad

    2013-04-01

    This investigation focuses on applications of GPR on structural detailing of a major tunnel under the River Medway in north Kent, UK. Construction of the tunnel was completed in 1996 and it carries a substantial volume of traffic between two major areas of Medway. The construction of the tunnel is an "immersed tube" tunnel type that connects a number of segments at immersion joint points. This investigation reports on utilisation of two separate GPR antenna systems at different frequencies in establishing structural details of the tunnel roof at immersion joints. The processed data compiled as a result of this investigation provided much needed information to tunnel engineers for forthcoming maintenance planning purposes. It also provided ample information in confirming rather doubted construction plans originally produced. The reported results are conclusive in terms of construction materials used (information was not originally available and needed confirmation) as well as establishing the required information on the formation of the tunnel roof joints. The presentation is complemented by providing detailed information of a complex process of adopting the GPR systems used in this endeavour.

  16. Haemophilus influenzae Disease (Including Hib) Symptoms

    Science.gov (United States)

    ... is considered invasive. Symptoms of pneumonia usually include: Fever and chills Cough Shortness of breath or difficulty breathing Sweating ... the blood. It can cause symptoms such as: Fever and chills Excessive tiredness Pain in the belly Nausea with ...

  17. What Does Long-Term Care Include?

    Medline Plus

    Full Text Available Video: "What Does Long-Term Care Include?" Long-term care involves a variety of services designed to meet a person's health or personal care needs during a short or long period of ...

  18. Births and deaths including fetal deaths

    Data.gov (United States)

    U.S. Department of Health & Human Services — Access to a variety of United States birth and death files including fetal deaths: Birth Files, 1968-2009; 1995-2005; Fetal death file, 1982-2005; Mortality files,...

  19. Including Indigenous Minorities in Decision-Making

    DEFF Research Database (Denmark)

    Pristed Nielsen, Helene

    Based on theories of public sphere participation and deliberative democracy, this book presents empirical results from a study of experiences with including Aboriginal and Maori groups in political decision-making in respectively Western Australia and New Zealand...

  20. A Detailed Immunohistochemical Analysis of a Large Series of Cervical and Vaginal Gastric-type Adenocarcinomas.

    Science.gov (United States)

    Carleton, Claire; Hoang, Lien; Sah, Shatrughan; Kiyokawa, Takako; Karamurzin, Yevgeniy S; Talia, Karen L; Park, Kay J; McCluggage, W Glenn

    2016-05-01

    Adenocarcinomas exhibiting gastric differentiation represent a recently described and uncommon subtype of non-human papillomavirus (HPV)-related cervical adenocarcinoma. They comprise a spectrum from a well-differentiated variant (adenoma malignum/mucinous variant of minimal deviation adenocarcinoma) to a more poorly differentiated overtly malignant form, generally referred to as gastric-type adenocarcinoma. Rarely, such tumors have also been described as primary vaginal neoplasms. Gastric-type adenocarcinomas exhibit considerable morphologic overlap with adenocarcinomas originating outside the female genital tract, especially mucinous adenocarcinomas arising in the pancreas and biliary tract. Moreover, they often metastasize to unusual sites, such as the ovary and peritoneum/omentum, where they can be mistaken for metastatic adenocarcinomas from other, nongynecologic sites. There is little information regarding the immunophenotype of gastric-type adenocarcinomas, and knowledge of this is important to aid in the distinction from other adenocarcinomas. In this study, we undertook a detailed immunohistochemical analysis of a large series of cervical (n=45) and vaginal (n=2) gastric-type adenocarcinomas. Markers included were cytokeratin (CK)7, CK20, CDX2, carcinoembryonic antigen, CA125, CA19.9, p16, estrogen receptor, progesterone receptor, MUC6, PAX8, PAX2, p53, hepatocyte nuclear factor 1 beta, carbonic anhydrase IX, human epidermal receptor 2 (HER2), and mismatch repair (MMR) proteins. All markers were classified as negative, focal (<50% of tumor cells positive), or diffuse (≥50% tumor cells positive) except for p53 (classified as "wild-type" or "mutation-type"), HER2 (scored using the College of American Pathologists guidelines for gastric carcinomas), and MMR proteins (categorized as retained or lost). There was positive staining with CK7 (47/47-45 diffuse, 2 focal), MUC6 (17/21-6 diffuse, 11 focal), carcinoembryonic antigen (25/31-12 diffuse, 13 focal

  1. Genes (including RNA editing information) - RMG | LSDB Archive [Life Science Database Archive metadata

    Lifescience Database Archive (English)

    Full Text Available [ Credits ] BLAST Search Image Search Home About Archive Update History Contact us RMG Genes... (including RNA editing information) Data detail Data name Genes (including RNA editing information...ase Site Policy | Contact Us Genes (including RNA editing information) - RMG | LSDB Archive ...

  2. Fitting the linear quadratic model to detailed data sets for different dose ranges

    Science.gov (United States)

    Garcia, L. M.; Leblanc, J.; Wilkins, D.; Raaphorst, G. P.

    2006-06-01

    Survival curve behaviour and degree of correspondence between the linear-quadratic (LQ) model and experimental data in an extensive dose range for high dose rates were analysed. Detailed clonogenic assays with irradiation given in 0.5 Gy increments and a total dose range varying from 10.5 to 16 Gy were performed. The cell lines investigated were: CHOAA8 (Chinese hamster fibroblast cells), U373MG (human glioblastoma cells), CP3 and DU145 (human prostate carcinoma cell lines). The analyses were based on χ2-statistics and Monte Carlo simulation of the experiments. A decline of LQ fit quality at very low doses (<2 Gy) is observed. This result can be explained by the hypersensitive effect observed in CHOAA8, U373MG and DU145 data and an adaptive-type response in the CP3 cell line. A clear improvement of the fit is discerned by removing the low dose data points. The fit worsening at high doses also shows that LQ cannot explain this region. This shows that the LQ model fits better the middle dose region of the survival curve. The analysis conducted in our study reveals a dose dependency of the LQ fit in different cell lines.

  3. Detailed modeling of the atmospheric degradation mechanism of very-short lived brominated species

    OpenAIRE

    G. Krysztofiak; Catoire, Valéry; Poulet, G.; Marécal, V.; Pirre, Michel; Louis, F.; Canneaux, S.; B. Josse

    2012-01-01

    International audience Detailed chemical reaction schemes for the atmospheric degradations of the very short-lived species (VSLS) bromoform (CHBr3) and dibromomethane (CH2Br2) have been established. These degradation schemes have been implemented in the meteorological/tracer transport model CATT-BRAMS used in the present case as pseudo one-dimensional model with chemistry of CH4, CO, HOx, NOx, NOy and Ox. They include the main possible reactions of the intermediate brominated peroxy radica...

  4. The Hybrid Detailed / Statistical Opacity Code SCO-RCG: New Developments and Applications

    OpenAIRE

    Pain, Jean-Christophe; Gilleron, Franck; Porcherot, Quentin; Blenski, Thomas

    2013-01-01

    We present the hybrid opacity code SCO-RCG which combines statistical approaches with fine-structure calculations. Radial integrals needed for the computation of detailed transition arrays are calculated by the code SCO (Super-configuration Code for Opacity), which calculates atomic structure at finite temperature and density, taking into account plasma effects on the wave-functions. Levels and spectral lines are then computed by an adapted RCG routine of R. D. Cowan. SCO-RCG now includes the...

  5. Detailed atmospheric abundance analysis of the optical counterpart of the IR source IRAS 16559-2957

    CERN Document Server

    Molina, R E

    2013-01-01

    We have undertaken a detailed abundance analysis of the optical counterpart of the IR source IRAS16559-2957 with the aim of confirming its possible post-AGB nature. The star shows solar metallicity and our investigation of a large number of elements including CNO and 12C/13C suggests that this object has experienced the first dredge-up and it is likely still at RGB stage.

  6. Gibraltar; Detailed Assessment Report of Observance of the Insurance Core Principles

    OpenAIRE

    International Monetary Fund

    2007-01-01

    This paper examines Gibraltar’s Detailed Assessment Report of the Observance of the Insurance Core Principles. Foreign establishments operating in Gibraltar are all European Union (EU)-based and therefore covered by EU directives, including the directive on freedom of establishment. A key reason for the growth in this sector is the ability of firms licensed in Gibraltar to passport their services to EU member states. The domestic insurance market is, however, quite small with a population i...

  7. Electric Power Monthly, August 1990. [Glossary included

    Energy Technology Data Exchange (ETDEWEB)

    1990-11-29

    The Electric Power Monthly (EPM) presents monthly summaries of electric utility statistics at the national, Census division, and State level. The purpose of this publication is to provide energy decisionmakers with accurate and timely information that may be used in forming various perspectives on electric issues that lie ahead. Data includes generation by energy source (coal, oil, gas, hydroelectric, and nuclear); generation by region; consumption of fossil fuels for power generation; sales of electric power, cost data; and unusual occurrences. A glossary is included.

  8. Synthesis and characterization of phase change memory cells

    Institute of Scientific and Technical Information of China (English)

    2009-01-01

    Phase change memory (PCM) cells based on Ge2Sb2Te5 were synthesized and investigated. Currentvoltage measurements demonstrated different final resistances. Transmission electron microscopy (TEM),high resolution electron microscopy (HREM) and the energy dispersive X-ray spectroscopy (EDS) analyses were used to characterize the microstructures of the PCM cells. The architectures,structures and defects in the cells including the deposited elemental distributions and the interfacial structures between electrodes and barrier layers were studied in detail.

  9. The Effects of Seductive Details in an Inflatable Planetarium

    Science.gov (United States)

    Gillette, Sean

    Astronomy is becoming a forgotten science, which is evident by its relatively low enrollment figures compared to biology, chemistry, and physics. A portable inflatable planetarium brings relevance back to astronomy and offers support to students and educators by simulating realistic astronomical environments. This study sought to determine if learning is improved in an inflatable planetarium by adhering to the design principles of the cognitive theory of multimedia learning (CTML), specifically the coherence principle, in an authentic classroom. Two groups of 5th grade students of similar ability were purposefully assigned using a 1-teacher-to-many-students format with mean lesson lengths of 34 minutes. The experimental group was differentiated with seductive details, defined as interesting but irrelevant facts that can distract learning. The control group ( n = 28), with seductive details excluded, outperformed the experimental group (n = 28), validating the coherence principle and producing a Cohen's effect size of medium practical significance (d = 0.4). These findings suggest that CTML, when applied to planetarium instruction, does increase student learning and that seductive details do have a negative effect on learning. An adult training project was created to instruct educators on the benefits of CTML in astronomy education. This study leads to positive social change by highlighting astronomy education while providing educators with design principles of CTML in authentic settings to maximize learning, aid in the creation of digital media (astronomical simulations/instructional lessons for planetariums) and provide valuable training for owners of inflatable planetariums with the eventual goal of increasing student enrollment of astronomy courses at the local level.

  10. Cell-Based Biosensors Principles and Applications

    CERN Document Server

    Wang, Ping

    2009-01-01

    Written by recognized experts the field, this leading-edge resource is the first book to systematically introduce the concept, technology, and development of cell-based biosensors. You find details on the latest cell-based biosensor models and novel micro-structure biosensor techniques. Taking an interdisciplinary approach, this unique volume presents the latest innovative applications of cell-based biosensors in a variety of biomedical fields. The book also explores future trends of cell-based biosensors, including integrated chips, nanotechnology and microfluidics. Over 140 illustrations hel

  11. Po Superconducting Magnet:detail of the windings

    CERN Multimedia

    1982-01-01

    The Po superconducting dipole was built as a prototype beam transport magnet for the SPS extracted proton beam Po. Its main features were: coil aperture 72 mm, length 5 m, room-temperature yoke, NbTi cable conductor impregnated with solder, nominal field 4.2 T at 4.7 K (87% of critical field). It reached its nominal field without any quench. The photo shows a detail of the inner layer winding before superposing the outer layer to form the complete coil of a pole. Worth noticing is the interleaved glass-epoxy sheet (white) with grooved channels for the flow of cooling helium. See also 8307552X.

  12. Soviet Illegal Whaling: The Devil and the Details

    OpenAIRE

    Ivashchenko, Yulia V.; Clapham, Phillip J.; Brownell, Jr. , Robert L.

    2011-01-01

    In 1948, the U.S.S.R. began a global campaign of illegal whaling that lasted for three decades and, together with the poorly managed “legal” whaling of other nations, seriously depleted whale populations. Although the general story of this whaling has been told and the catch record largely corrected for the Southern Hemisphere, major gaps remain in the North Pacific. Furthermore, little attention has been paid to the details of this system or its economic context. Using interviews with for...

  13. GRB 050822: Detailed analysis of an XRF observed by Swift

    OpenAIRE

    Godet, O; Page, K. L.; Osborne, J.; B. Zhang; Burrows, D.N.; O'Brien, P. T.; Hill, J. E.; Racusin, J.; Beardmore, A. P.; Goad, M. R.; Falcone, A; Morris, D. C.; Ziaeepour, H.

    2007-01-01

    We report on the temporal and spectral characteristics of the early X-ray emission from the GRB 050822 as observed by Swift. This burst is likely to be an XRF showing major X-ray flares in its XRT light-curve. The quality of the data allows a detailed spectral analysis of the early afterglow in the X-ray band. During the X-ray flares, a positive correlation between the count rate and the spectral hardness (i.e. the higher the count rate, the harder the spectrum) is clearly seen for the X-ray ...

  14. Aircraft wing structural detail design (wing, aileron, flaps, and subsystems)

    Science.gov (United States)

    Downs, Robert; Zable, Mike; Hughes, James; Heiser, Terry; Adrian, Kenneth

    1993-01-01

    The goal of this project was to design, in detail, the wing, flaps, and ailerons for a primary flight trainer. Integrated in this design are provisions for the fuel system, the electrical system, and the fuselage/cabin carry-through interface structure. This conceptual design displays the general arrangement of all major components in the wing structure, taking into consideration the requirements set forth by the appropriate sections of Federal Aviation Regulation Part 23 (FAR23) as well as those established in the statement of work.

  15. Number plates – confidentiality and your personal details

    CERN Multimedia

    Since the 15th May 2008, Geneva’s ‘Service des automobiles et de la navigation’ has offered the possibility of finding out the name and address of owners of vehicles registered in the Canton of Geneva simply by sending a SMS of the car’s number plate to 939.However, owners of number plates registered in Geneva can block the divulgence of their personal details by filling out the following form, available at:http://www.ge.ch/san/doc/sms-confidentialite.pdf

  16. Detail study of SiC MOSFET switching characteristics

    DEFF Research Database (Denmark)

    Li, Helong; Munk-Nielsen, Stig

    2014-01-01

    This paper makes detail study of the latest SiC MOSFETs switching characteristics in relation to gate driver maximum current, gate resistance, common source inductance and parasitic switching loop inductance. The switching performance of SiC MOSFETs in terms of turn on and turn off voltage...... and current are presented. Switching losses analysis is made according to the experiment results. The switching characteristics study and switching losses analysis could give some guidelines of gate driver IC and gate resistance selection, switching losses estimation and circuit design of SiC MOSFETs....

  17. Detailed Atmosphere Model Fits to Disk-Dominated ULX Spectra

    OpenAIRE

    Hui, Y; Krolik, Julian H.

    2008-01-01

    We have chosen 6 Ultra-Luminous X-ray sources from the {\\it XMM-Newton} archive whose spectra have high signal-to-noise and can be fitted solely with a disk model without requiring any power-law component. To estimate systematic errors in the inferred parameters, we fit every spectrum to two different disk models, one based on local blackbody emission (KERRBB) and one based on detailed atmosphere modelling (BHSPEC). Both incorporate full general relativistic treatment of the disk surface brig...

  18. 34 CFR 300.20 - Include.

    Science.gov (United States)

    2010-07-01

    ... 34 Education 2 2010-07-01 2010-07-01 false Include. 300.20 Section 300.20 Education Regulations of the Offices of the Department of Education (Continued) OFFICE OF SPECIAL EDUCATION AND REHABILITATIVE SERVICES, DEPARTMENT OF EDUCATION ASSISTANCE TO STATES FOR THE EDUCATION OF CHILDREN WITH...

  19. 46 CFR 289.2 - Vessels included.

    Science.gov (United States)

    2010-10-01

    ... 46 Shipping 8 2010-10-01 2010-10-01 false Vessels included. 289.2 Section 289.2 Shipping MARITIME ADMINISTRATION, DEPARTMENT OF TRANSPORTATION REGULATIONS AFFECTING SUBSIDIZED VESSELS AND OPERATORS INSURANCE OF... of this part are: (a) All vessels which may in the future be constructed or sold with...

  20. 42 CFR 409.10 - Included services.

    Science.gov (United States)

    2010-10-01

    ... 42 Public Health 2 2010-10-01 2010-10-01 false Included services. 409.10 Section 409.10 Public Health CENTERS FOR MEDICARE & MEDICAID SERVICES, DEPARTMENT OF HEALTH AND HUMAN SERVICES MEDICARE PROGRAM HOSPITAL INSURANCE BENEFITS Inpatient Hospital Services and Inpatient Critical Access Hospital...

  1. Deciphering the details of RNA aminoglycoside interactions: from atomistic models to biotechnological applications

    Energy Technology Data Exchange (ETDEWEB)

    Ilgu, Muslum [Iowa State Univ., Ames, IA (United States)

    2012-01-01

    A detailed study was done of the neomycin-B RNA aptamer for determining its selectivity and binding ability to both neomycin– and kanamycin-class aminoglycosides. A novel method to increase drug concentrations in cells for more efficiently killing is described. To test the method, a bacterial model system was adopted and several small RNA molecules interacting with aminoglycosides were cloned downstream of T7 RNA polymerase promoter in an expression vector. Then, the growth analysis of E. coli expressing aptamers was observed for 12-hour period. Our analysis indicated that aptamers helped to increase the intracellular concentration of aminoglycosides thereby increasing their efficacy.

  2. A quick overview of membrane computing with some details about spiking neural P systems

    Institute of Scientific and Technical Information of China (English)

    Gheorghe Pǎun

    2007-01-01

    We briefly present the basic elements of membrane computing,a branch of natural computing inspired by the structure and functioning of living cells,then we give some details about spiking neural P systems,a class of membrane systems recently introduced,with motivations related to the way neurons communicate by means of spikes.In both cases,of general P systems and of spiking neural P systems,we introduce the fundamental concepts,give a few examples,then recall the types of results and of applications.A series of bibliographical references are provided.

  3. Occlusion Culling Algorithm Using Prefetching and Adaptive Level of Detail Technique

    Institute of Scientific and Technical Information of China (English)

    ZHENG Fu-ren; ZHAN Shou-yi; Yang Bing

    2006-01-01

    A novel approach that integrates occlusion culling within the view-dependent rendering framework is proposed. The algorithm uses the prioritized-layered projection(PLP) algorithm to occlude those obscured objects, and uses an approximate visibility technique to accurately and efficiently determine which objects will be visible in the coming future and prefetch those objects from disk before they are rendered. View-dependent rendering technique provides the ability to change level of detail over the surface seamlessly and smoothly in real-time according to cell solidity value.

  4. Manufacturing details by Neutron Radiography of Archaeological Pottery

    Energy Technology Data Exchange (ETDEWEB)

    Bernedo, Alfredo Victor Bellido; Latini, Rose Mary [Universidade Federal Fluminense (UFF), Niteroi, RJ (Brazil); Souza, Maria Ines Silvani; Vinagre Filho, Ubirajara Maribondo [Instituto de Engenharia Nuclear (IEN/CNEN-RJ), Rio de Janeiro, RJ (Brazil)

    2011-07-01

    Full text: The aim of the present work was to investigate manufacturing details of archaeological pot-sherds ceramics by Neutron Radiography. Pottery is perhaps the most important artefact found in excavation. Its archaeological importance relies on the fact that it can reveal cultural traditions and commercial influences in ancient communities. These pottery was recently discovered in archaeological earth circular structures sites in Acre state Brazil and the characteristics of clay used in their manufacture have been studied by modern scientific techniques such as Instrumental Neutron Activation Analysis (INAA), Thermoluminescence Dating and Moessbauer Spectroscopy. Different fragments of pottery were submitted to a neutron flux of the order of 10{sup 5}n.cm{sup -2}2:s{sup -1} for 3 minutes in the research reactor Argonauta at the Instituto de Engenharia Nuclear/CNEN. Digital processing techniques using imaging plate were applied to the image of the selected sample. The Neutrongraphy shows two different manufacturing details: palette and rollers. The fragment made by the technique of palette show a homogeneous mass and the neutrongraphy of ceramic fragments made by the technique of the rollers, pottery funeral, can be seen horizontal traces of the junction of rollers, overlapping, forming layers supported on each other. This technique allows you to create more stable structures. Thus, both the technique of the pallet as the roller can be characterized by Neutron Radiography. (author)

  5. Effects of Geometric Details on Slat Noise Generation and Propagation

    Science.gov (United States)

    Khorrami, Mehdi R.; Lockard, David P.

    2009-01-01

    The relevance of geometric details to the generation and propagation of noise from leading-edge slats is considered. Typically, such details are omitted in computational simulations and model-scale experiments thereby creating ambiguities in comparisons with acoustic results from flight tests. The current study uses two-dimensional, computational simulations in conjunction with a Ffowcs Williams-Hawkings (FW-H) solver to investigate the effects of previously neglected slat "bulb" and "blade" seals on the local flow field and the associated acoustic radiation. The computations show that the presence of the "blade" seal at the cusp in the simulated geometry significantly changes the slat cove flow dynamics, reduces the amplitudes of the radiated sound, and to a lesser extent, alters the directivity beneath the airfoil. Furthermore, the computations suggest that a modest extension of the baseline "blade" seal further enhances the suppression of slat noise. As a side issue, the utility and equivalence of FW-H methodology for calculating far-field noise as opposed to a more direct approach is examined and demonstrated.

  6. The detailed nature of active central cluster galaxies

    CERN Document Server

    Loubser, S I

    2013-01-01

    We present detailed integral field unit (IFU) observations of the central few kiloparsecs of the ionised nebulae surrounding four active central cluster galaxies (CCGs) in cooling flow clusters (Abell 0496, 0780, 1644 and 2052). Our sample consists of CCGs with H{\\alpha} filaments, and have existing data from the X-ray regime available. Here, we present the detailed optical emission-line (and simultaneous absorption line) data over a broad wavelength range to probe the dominant ionisation processes, excitation sources, morphology and kinematics of the hot gas (as well as the morphology and kinematics of the stars). This, combined with the other multiwavelength data, will form a complete view of the different phases (hot and cold gas and stars) and how they interact in the processes of star formation and feedback detected in central galaxies in cooling flow clusters, as well as the influence of the host cluster. We derive the optical dust extinction maps of the four nebulae. We also derive a range of different...

  7. Detailed kinetic modeling study of n-pentanol oxidation

    KAUST Repository

    Heufer, Karl Alexander

    2012-10-18

    To help overcome the world\\'s dependence upon fossil fuels, suitable biofuels are promising alternatives that can be used in the transportation sector. Recent research on internal combustion engines shows that short alcoholic fuels (e.g., ethanol or n-butanol) have reduced pollutant emissions and increased knock resistance compared to fossil fuels. Although higher molecular weight alcohols (e.g., n-pentanol and n-hexanol) exhibit higher reactivity that lowers their knock resistance, they are suitable for diesel engines or advanced engine concepts, such as homogeneous charge compression ignition (HCCI), where higher reactivity at lower temperatures is necessary for engine operation. The present study presents a detailed kinetic model for n-pentanol based on modeling rules previously presented for n-butanol. This approach was initially validated using quantum chemistry calculations to verify the most stable n-pentanol conformation and to obtain C-H and C-C bond dissociation energies. The proposed model has been validated against ignition delay time data, speciation data from a jet-stirred reactor, and laminar flame velocity measurements. Overall, the model shows good agreement with the experiments and permits a detailed discussion of the differences between alcohols and alkanes. © 2012 American Chemical Society.

  8. Manufacturing details by Neutron Radiography of Archaeological Pottery

    International Nuclear Information System (INIS)

    Full text: The aim of the present work was to investigate manufacturing details of archaeological pot-sherds ceramics by Neutron Radiography. Pottery is perhaps the most important artefact found in excavation. Its archaeological importance relies on the fact that it can reveal cultural traditions and commercial influences in ancient communities. These pottery was recently discovered in archaeological earth circular structures sites in Acre state Brazil and the characteristics of clay used in their manufacture have been studied by modern scientific techniques such as Instrumental Neutron Activation Analysis (INAA), Thermoluminescence Dating and Moessbauer Spectroscopy. Different fragments of pottery were submitted to a neutron flux of the order of 105n.cm-22:s-1 for 3 minutes in the research reactor Argonauta at the Instituto de Engenharia Nuclear/CNEN. Digital processing techniques using imaging plate were applied to the image of the selected sample. The Neutrongraphy shows two different manufacturing details: palette and rollers. The fragment made by the technique of palette show a homogeneous mass and the neutrongraphy of ceramic fragments made by the technique of the rollers, pottery funeral, can be seen horizontal traces of the junction of rollers, overlapping, forming layers supported on each other. This technique allows you to create more stable structures. Thus, both the technique of the pallet as the roller can be characterized by Neutron Radiography. (author)

  9. Animation-Based Learning in Geology: Impact of Animations Coupled with Seductive Details

    Science.gov (United States)

    Clayton, Rodney L.

    Research is not clear on how to address the difficulty students have conceptualizing geologic processes and phenomena. This study investigated how animations coupled with seductive details effect learners' situational interest and emotions. A quantitative quasi-experimental study employing an independent-measures factorial design was used. The participants included a convenience sampling of 102 undergraduates. There was a main effect of seductive details on comprehension, F (2, 94) = 10.02, p animations significantly increased learning performance compared to illustrations, t(94) = 2.03, p animations to illustrations (DeltaM = 7.93, p = .007). There was a significant effect of seductive details on perceived interest after controlling for spatial ability and prior knowledge, F (2, 94) = 3.65, p = .030, etap2 = .072. Learners' prior knowledge also had a significant effect on perceived interest, F (1, 94) = 4.74, p = .032, etap2 = .048. There appeared to be no effect of presentational modality on perceived interest, F .05. Considering the inconsistent results of studies, and the potential impact of affective factors, further research is needed to evaluate the efficacy of animations and the use of seductive details under different learning conditions.

  10. [Biophysical Characterization of Biopharmaceuticals, Including Antibody Drugs].

    Science.gov (United States)

    Uchiyama, Susumu

    2016-01-01

    Biopharmaceuticals, including antibody drugs, are now popular because of their high specificity with low adverse effects, especially in the treatment of cancer and autoimmune diseases. However, because the active pharmaceutical ingredients of biopharmaceuticals are proteins, biophysical characterization of these therapeutic proteins should be required. In this manuscript, methods of chemical and physical characterization of therapeutic proteins are described. In terms of chemical characterization, analysis of chemical modifications of the constituent amino acids is explained. Physical characterization includes higher order structural analysis and assessment of protein aggregates. Quantification methods of aggregates with different sizes, recently encouraged by the U.S. Food and Drug Administration (FDA), are introduced. As for the stability of therapeutic proteins, the importance of chemical and physical stability is explained. Finally, the contribution of colloidal and structural stability to the production of an antibody drug less prone to aggregation is introduced.

  11. Electric power monthly, September 1990. [Glossary included

    Energy Technology Data Exchange (ETDEWEB)

    1990-12-17

    The purpose of this report is to provide energy decision makers with accurate and timely information that may be used in forming various perspectives on electric issues. The power plants considered include coal, petroleum, natural gas, hydroelectric, and nuclear power plants. Data are presented for power generation, fuel consumption, fuel receipts and cost, sales of electricity, and unusual occurrences at power plants. Data are compared at the national, Census division, and state levels. 4 figs., 52 tabs. (CK)

  12. Including knowledge creation & enabling in risk management

    OpenAIRE

    Hop, Eirik

    2015-01-01

    As a contribution to Statoil Technical Efficiency Programme (STEP), has the thesis looked at how to improve the risk management process in Statoil ASA. Through theoretical research was the primary research question created: “How can knowledge creation & enabling improve our understanding of risk management?” To create a theoretical foundation, the thesis looked at principles, methods, and models for the adequate assessment and management of risk. This includes a new perspective on ri...

  13. Rotor assembly including superconducting magnetic coil

    Energy Technology Data Exchange (ETDEWEB)

    Snitchler, Gregory L. (Shrewsbury, MA); Gamble, Bruce B. (Wellesley, MA); Voccio, John P. (Somerville, MA)

    2003-01-01

    Superconducting coils and methods of manufacture include a superconductor tape wound concentrically about and disposed along an axis of the coil to define an opening having a dimension which gradually decreases, in the direction along the axis, from a first end to a second end of the coil. Each turn of the superconductor tape has a broad surface maintained substantially parallel to the axis of the coil.

  14. Including heavy flavour production in PDF fits

    OpenAIRE

    A.M. Cooper-Sarkar

    2007-01-01

    AT HERA heavy quarks may contribute up to 30% of the structure function $F_2$. The introduction of heavy quarks requires an extension of the DGLAP formalism. The effect of using different heavy flavour number schemes, and different approaches to the running of $\\alpha_s$, are compared using the ZEUS PDF fit formalism. The potential of including charm data in the fit is explored, using $D^*$ double differential cross-sections rather than the inclusive quantity $F_2^{c\\bar{c}}$.

  15. Detailed free span assessment for Mexilhao flow lines

    Energy Technology Data Exchange (ETDEWEB)

    Pereira, Antonio; Franco, Luciano; Eigbe, Uwa; BomfimSilva, Carlos [INTECSEA, Houston, TX (United States); Escudero, Carlos [PETROBRAS S.A., Rio de Janeiro, RJ (Brazil)

    2009-07-01

    The subsea gas production system of Mexilhao Field SPS-35, Santos Basin, offshore Brazil, is composed basically of two rigid 12.75 inches production flow lines approximately 21 km long installed in a fairly rough seabed. During the basic design, the free span assessment was performed considering the maximum allowable free span length determined by the response model proposed by DNV-RP-F105. This approach resulted in a large number of predicted free span requiring corrections, leading to a higher capital cost for the project. In this sense, a detailed free span VIV fatigue assessment was proposed, considering multi-spans and multi-mode effects and also the post lay survey data. The assessment followed the DNV-RP-F105 recommendations for multi-spans and multi-mode effects, using Finite Element Analysis to determine the natural frequencies, mode shapes and corresponding stresses associated with the mode shapes. The assessment was performed in three stages, the first during the detailed design as part of the bottom roughness analysis using the expected residual pipelay tension. The second stage was performed after pipelay, considering the post-lay survey data, where the actual requirements for span correction were determined. Actual pipelay tension was used and seabed soil stiffness adjusted in the model to match the as-laid pipeline profile obtained from the survey data. The first and second stage assessments are seamlessly automated to speed up the evaluation process and allow for quick response in the field, which was important to keep the construction vessel time minimized. The third stage was performed once the corrections of the spans were made and the purpose was to confirm that the new pipeline configuration along the supported spans had sufficient fatigue life for the temporary and operational phases. For the assessment of all three stages, the probability of occurrence and directionality of the near bottom current was considered to improve prediction of the

  16. A kicked quantum system including the continuum

    International Nuclear Information System (INIS)

    The behaviour of a quantum particle in a separable one-term potential with three-dimensional form factor is investigated under the influence of an external force which alters the potential strength periodically or quasiperiodically. The unperturbed system possesses one bound state and a continuum of scattering states which has treated almost analytically. First numerical results, fully including the emission channel, indicate, for certain parameter combinations with commensurate or incommensurate frequency ratios, either a regular or an irregular dynamical behaviour of the system. 17 refs.; 3 figs

  17. Drug delivery device including electrolytic pump

    KAUST Repository

    Foulds, Ian G.

    2016-03-31

    Systems and methods are provided for a drug delivery device and use of the device for drug delivery. In various aspects, the drug delivery device combines a “solid drug in reservoir” (SDR) system with an electrolytic pump. In various aspects an improved electrolytic pump is provided including, in particular, an improved electrolytic pump for use with a drug delivery device, for example an implantable drug delivery device. A catalytic reformer can be incorporated in a periodically pulsed electrolytic pump to provide stable pumping performance and reduced actuation cycle.

  18. Musculoskeletal ultrasound including definitions for ultrasonographic pathology

    DEFF Research Database (Denmark)

    Wakefield, RJ; Balint, PV; Szkudlarek, Marcin;

    2005-01-01

    Ultrasound (US) has great potential as an outcome in rheumatoid arthritis trials for detecting bone erosions, synovitis, tendon disease, and enthesopathy. It has a number of distinct advantages over magnetic resonance imaging, including good patient tolerability and ability to scan multiple joints...... pathologies. This article presents the first report from the OMERACT ultrasound special interest group, which has compared US against the criteria of the OMERACT filter. Also proposed for the first time are consensus US definitions for common pathological lesions seen in patients with inflammatory arthritis....

  19. Ultrastructural Details of Cryptosporidium parvum Development in Calf Intestine

    Directory of Open Access Journals (Sweden)

    Mª José Rosales

    1998-11-01

    Full Text Available Cryptosporidium parvum and C. muris appear to be different species found in calves, with different oocysts size and distribution on the gastrointestinal tract. This work presents new images of C. parvum ultrastructure in calf intestine, mainly its development in nonmicrovillous cells and the presence of microtubular structures in the membrane enveloping the macrogamonts and immature oocysts.

  20. Terminology and details of the diagnostic process for testis cancer.

    LENUS (Irish Health Repository)

    Connolly, Stephen S

    2011-03-01

    We examined the process and causes of diagnostic delay, defined as the interval from symptom onset to diagnosis, for testis (germ cell) cancer and the change with time. Diagnostic delay influences disease burden and may be subdivided into symptomatic interval, defined as symptom onset to first presentation, and diagnostic interval, defined as first presentation to diagnosis.

  1. A Detailed Strategy for Managing Corporation Cyber War Security

    Directory of Open Access Journals (Sweden)

    Walid Al-Ahmad

    2015-05-01

    Full Text Available Modern corporations depend heavily on information and communication technologies and are becoming increasingly interconnected locally and internationally. This interconnectedness and dependency on information technology make corporations vulnerable to cyber attacks. Corporate managers therefore need to understand the growing cyber war threats and implement appropriate strategies to mitigate the risks. This research work is an attempt to develop a generic and detailed strategy to assist corporations in managing the cyber war security. The implementation of such a strategy will definitely lead to a more secure business environment and as a result will attract foreign investments to the Arab countries in the Middle East. Such a strategy can be considered as a first step toward protecting corporations from cyber war threats in an effective manner.

  2. A detailed study of patent system for protection of inventions

    Directory of Open Access Journals (Sweden)

    Tulasi G

    2008-01-01

    Full Text Available Creations of brain are called intellect. Since these creations have good commercial value, are called as property. Inventions are intellectual property and can be protected by patents provided the invention is novel, non-obvious, useful and enabled. To have fare trade among member countries, World Trade Organisation proposed TRIPS agreement. India had taken necessary initiation by signing the World Trade Organisation agreement and transformed to global needs. The aim of this article is to enlighten pharmaceutical professionals especially in the field of research and development about planning inventions by thorough review of prior-art, which saves time and money. A thorough understanding is made possible by providing details of origin; present governing bodies, their role along with the Act that is safeguarding the patent system.

  3. Detail analysis of fusion neutronics benchmark experiment for iron

    Energy Technology Data Exchange (ETDEWEB)

    Konno, Chikara [Fusion Research and Development Directorate, Japan Atomic Energy Agency, Tokai-mura, Naka-gun, Ibaraki-ken 319-1195 (Japan)], E-mail: konno.chikara@jaea.go.jp; Ochiai, Kentaro [Fusion Research and Development Directorate, Japan Atomic Energy Agency, Tokai-mura, Naka-gun, Ibaraki-ken 319-1195 (Japan); Wada, Masayuki [Japan Computer System, Mito 310-0805 (Japan); Takakura, Kosuke; Sato, Satoshi [Fusion Research and Development Directorate, Japan Atomic Energy Agency, Tokai-mura, Naka-gun, Ibaraki-ken 319-1195 (Japan)

    2009-06-15

    In order to specify the best nuclear data on iron, the fusion neutronics benchmark experiment on iron at Japan Atomic Energy Agency (JAEA)/Fusion Neutronics Source (FNS) was analyzed in detail with MCNP-4C and the latest nuclear data libraries, JENDL-3.3, FENDL-2.1, JEFF-3.1 and ENDF/B-VII.0. As a result, totally the calculation result with ENDF/B-VII.0 agreed with the measurement best, except that it underestimated the measured neutron flux above 10 MeV with the depth. It was noted that the calculation result with JENDL-3.3 overestimated the measured neutrons below a few keV. Through the DORT calculations based on the iron data in ENDF/B-VII.0, it was found out that the first inelastic scattering cross-section data of {sup 57}Fe in JENDL-3.3 caused the overestimation.

  4. A detailed and verified wind resource atlas for Denmark

    Energy Technology Data Exchange (ETDEWEB)

    Mortensen, N.G.; Landberg, L.; Rathmann, O.; Nielsen, M.N. [Risoe National Lab., Roskilde (Denmark); Nielsen, P. [Energy and Environmental Data, Aalberg (Denmark)

    1999-03-01

    A detailed and reliable wind resource atlas covering the entire land area of Denmark has been established. Key words of the methodology are wind atlas analysis, interpolation of wind atlas data sets, automated generation of digital terrain descriptions and modelling of local wind climates. The atlas contains wind speed and direction distributions, as well as mean energy densities of the wind, for 12 sectors and four heights above ground level: 25, 45, 70 and 100 m. The spatial resolution is 200 meters in the horizontal. The atlas has been verified by comparison with actual wind turbine power productions from over 1200 turbines. More than 80% of these turbines were predicted to within 10%. The atlas will become available on CD-ROM and on the Internet. (au)

  5. The AECL study for an intense neutron - generator (technical details)

    International Nuclear Information System (INIS)

    The AECL study for an intense neutron-generator has been in progress for two years. Recently the scientific and technical details and the conceptual designs were compiled in a report supporting proposals addressed to AECL's Board of Directors for further work. The compilation is being issued in this form to permit further discussion of the technical aspects. However readers are asked to appreciate that it was written primarily for an AECL audience, and specifically that those chapters giving tentative information about costs, the rate of investment and similar items have been omitted or modified, many references have been made to interim internal reports in order to complete the local documentation, but these references do not imply that the reports themselves can be made generally available. (author)

  6. The AECL study for an intense neutron - generator (technical details)

    Energy Technology Data Exchange (ETDEWEB)

    Bartholomew, G.A.; Tunnicliffe, P.R

    1966-07-01

    The AECL study for an intense neutron-generator has been in progress for two years. Recently the scientific and technical details and the conceptual designs were compiled in a report supporting proposals addressed to AECL's Board of Directors for further work. The compilation is being issued in this form to permit further discussion of the technical aspects. However readers are asked to appreciate that it was written primarily for an AECL audience, and specifically that those chapters giving tentative information about costs, the rate of investment and similar items have been omitted or modified, many references have been made to interim internal reports in order to complete the local documentation, but these references do not imply that the reports themselves can be made generally available. (author)

  7. Particle Simulation Research on the Detailed Process of Bubble Generation

    Institute of Scientific and Technical Information of China (English)

    马燕云; 常文蔚; 银燕; 曹莉华; 岳宗五

    2001-01-01

    The detailed process of bubble generation in laser-plasma interaction has been studied by particle simulation. During the interaction between laser and plasma, a strong electrostatic field caused by charge separation is observed, whose structure looks like a potential well. Electrons are accelerated to 10 MeV within some tens of femtoseconds when the laser intensity is 5× 1018 W/cm2. After many electrons escape from the potentiaLwell-like structure, electron cavitons are generated. Because of laser filamentation, the intensity of the laser becomes very asymmetric, the caviton structure is squeezed and deformed, and then electron bubbles are generated, whichfinally results in ion bubble formation. It is the electron caviton and laser beam filamentation that cause bubble generation. The i0 MeV superthermal electron generation accompanied by the electron caviton is attributed to EV . E heating, which is a locally oscillating electron heating mechanism.

  8. Detailed HI kinematics of Tully-Fisher calibrator galaxies

    CERN Document Server

    Ponomareva, Anastasia A; Bosma, Albert

    2016-01-01

    We present spatially-resolved HI kinematics of 32 spiral galaxies which have Cepheid or/and Tip of the Red Giant Branch distances, and define a calibrator sample for the Tully-Fisher relation. The interferometric HI data for this sample were collected from available archives and supplemented with new GMRT observations. This paper describes an uniform analysis of the HI kinematics of this inhomogeneous data set. Our main result is an atlas for our calibrator sample that presents global HI profiles, integrated HI column-density maps, HI surface density profiles and, most importantly, detailed kinematic information in the form of high-quality rotation curves derived from highly-resolved, two-dimensional velocity fields and position-velocity diagrams.

  9. Detailed analysis of observed antiprotons in cosmic rays

    Directory of Open Access Journals (Sweden)

    P Davoudifar

    2009-12-01

    Full Text Available In the present work, the origin of antiprotons observed in cosmic rays (above the atmosphere is analyzed in details. We have considered the origin of the primaries, (which their interactions with the interstellar medium is one of the most important sources of antiprotons is a supernova type II then used a diffusion model for their propagation. We have used the latest parameterization for antiproton production cross section in pp collisions (instead of well known parameterization introduced by Tan et al. as well as our calculated residence time for primaries. The resulted intensity shows the secondary antiprotons produced in pp collisions in the galaxy, have a high population as one can not consider an excess for extragalactic antiprotons. Also there is a high degree of uncertainty in different parameters.

  10. Devil in the Details: A Critical Review of "Theoretical Loss".

    Science.gov (United States)

    Tom, Matthew A; Shaffer, Howard J

    2016-09-01

    In their review of Internet gambling studies, Auer and Griffiths (J Gambl Stud 30(4), 879-887, 2014) question the validity of using bet size as an indicator of gambling intensity. Instead, in that review and in a response (Auer and Griffiths, J Gambl Stud 31(3), 921-931, 2015) to a previous comment (Braverman et al., J Gambl Stud 31(2), 359-366, 2015), Auer and Griffiths suggested using "theoretical loss" as a preferable measure of gambling intensity. This comment extends and advances the discussion about measures of gambling intensity. In this paper, we describe previously identified problems that Auer and Griffiths need to address to sustain theoretical loss as a viable measure of gambling intensity and add details to the discussion that demonstrate difficulties associated with the use of theoretical loss with certain gambling games. PMID:26670791

  11. San Joaquin-Tulare Conjunctive Use Model: Detailed model description

    Energy Technology Data Exchange (ETDEWEB)

    Quinn, N.W.T.

    1992-03-01

    The San Joaquin - Tulare Conjunctive Use Model (SANTUCM) was originally developed for the San Joaquin Valley Drainage Program to evaluate possible scenarios for long-term management of drainage and drainage - related problems in the western San Joaquin Valley of California. A unique aspect of this model is its coupling of a surface water delivery and reservoir operations model with a regional groundwater model. The model also performs salinity balances along the tributaries and along the main stem of the San Joaquin River to allow assessment of compliance with State Water Resources Control Board water quality objectives for the San Joaquin River. This document is a detailed description of the various subroutines, variables and parameters used in the model.

  12. A Comparison of Detailed Level and Superconfiguration Models of Neon

    Energy Technology Data Exchange (ETDEWEB)

    Hansen, S B; Fournier, K B; Bauche-Arnoult, C; Bauche, J; Peyrusse, O

    2005-01-03

    The superconfiguration (SC) approach to collisional-radiative modeling can significantly decrease the computational demands of finding non-LTE level populations in complex systems. However, it has not yet been fully determined whether the statistical averaging of SC models leads to a significant loss of accuracy. The present work compares results from two independent models: a detailed-level accounting (DLA) model based on HULLAC data and the SC model MOST. The relatively simple level structures of the K- and L-shell ions of the neon test system ensure a tractable number of levels in the DLA model but challenge the statistical assumptions of the SC approach. Nonetheless, we find fair agreement between the two models for average ion charges, SC populations, and various effective temperatures.

  13. Horava-Lifshitz early universe phase transition beyond detailed balance

    Energy Technology Data Exchange (ETDEWEB)

    Kheyri, F.; Khodadi, M.; Sepangi, H.R. [Shahid Beheshti University, Department of Physics, Tehran (Iran, Islamic Republic of)

    2013-01-15

    The early universe is believed to have undergone a QCD phase transition to hadrons at about 10 {mu}s after the big bang. We study such a transition in the context of the non-detailed balance Horava-Lifshitz theory by investigating the effects of the dynamical coupling constant {lambda} in a flat universe. The evolution of the relevant physical quantities, namely the energy density {rho}, temperature T, scale factor a and the Hubble parameter H is investigated before, during and after the phase transition, assumed to be of first order. Also, in view of the recent lattice QCD simulations data, we study a cross-over phase transition of the early universe whose results are based on two different sets of lattice data. (orig.)

  14. Detailed structure of spinning detonation in a circular tube

    Energy Technology Data Exchange (ETDEWEB)

    Tsuboi, N. [Space Transportation Engineering Department, Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency, Yoshinodai 3-1-1, Sagamihara, Kanagawa 229-8510 (Japan); Eto, K.; Hayashi, A.K. [Department of Mechanical Engineering, Aoyama Gakuin University, Fuchinobe 5-10-1, Sagamihara, Kanagawa 229-8558 (Japan)

    2007-04-15

    A single spinning detonation wave propagating in a circular tube, discovered experimentally in 1926, is simulated three-dimensionally with a detailed chemical reaction mechanism. The detonation front obtained numerically rotates periodically with a Mach leg, whiskers, and a transverse detonation. A long pressure trail, which is distributed from the transverse detonation to downstream, was reproduced, clearly showing that the pressure trail also spins synchronously with the transverse detonation. The formation of an unburned gas pocket behind the detonation front was not observed in the present simulations because the rotating transverse detonation completely consumed the unburned gas. The calculated profiles of instantaneous OH mass fraction have a keystone shape behind the detonation front. The numerical results for pitch, track angle, Mach stem angle, and incident shock angle on the tube wall agree well with the experimental results. (author)

  15. Details of a Successful Clinical Decision Support System

    Science.gov (United States)

    Friedlin, Jeff; Dexter, Paul R.; Overhage, J. Marc

    2007-01-01

    Computerized physician order entry (CPOE) with clinical decision support (CDS) is regarded as one of the most effective ways to improve the quality of health care and increase patient safety. As electronic medical records become more available, such systems will increasingly become the method of choice to achieve these goals. Creating a CPOE/CDS system is a complex task, and some fail despite time consuming and expensive development. The CPOE system at the Regenstrief Institute incorporates sophisticated CDS and is one of the oldest and most successful in the U.S. Many years in development, it is currently used by hundreds of providers. Our well established, successful system can serve as a template or model for the future development of similar systems. We recently completed a full analysis of our CPOE/CDS system and present details of its structure, functionality and contents. PMID:18693837

  16. Detailed design of 700 MWe steam generator features

    Energy Technology Data Exchange (ETDEWEB)

    Korde, M.; John, B. [Nuclear Power Corp. of India Ltd., Mumbai, Maharashtra (India)]. E-mail: bjohn@npcil.co.in

    2006-07-01

    The next stage in the Indian nuclear power programme consists of building 700 MWe Indian Pressurized Heavy Water Reactor (IPHWR) units. This involves up-rating of all the plant equipment like reactor, steam generators (SGs), turbo-generator, major pumps, etc. The SG used in the current generation of IPHWRs, which have an electrical power of 540 MWe, is a mushroom type, inverted U-tube, natural-circulation SG. The 700 MWe SG design has the same the tube diameter, tube pitch and outer diameter of the steam generator sections as the 434 MWth SG, with certain changes in geometry of the feed header, flow restrictor in the downcomer and flow distribution plate. The changes resulted in a 30% increase in steam flow rate while maintaining the same circulation ratio. The paper describes detailing of these changes using a CFD code for optimizing the flow field. (author)

  17. Inlet Guide Vane Wakes Including Rotor Effects

    Science.gov (United States)

    Johnston, R. T.; Fleeter, S.

    2001-02-01

    Fundamental experiments are described directed at the investigation of forcing functions generated by an inlet guide vane (IGV) row, including interactions with the downstream rotor, for application to turbomachine forced response design systems. The experiments are performed in a high-speed research fan facility comprised of an IGV row upstream of a rotor. IGV-rotor axial spacing is variable, with the IGV row able to be indexed circumferentially, thereby allowing measurements to be made across several IGV wakes. With an IGV relative Mach number of 0.29, measurements include the IGV wake pressure and velocity fields for three IGV-rotor axial spacings. The decay characteristics of the IGV wakes are compared to the Majjigi and Gliebe empirical correlations. After Fourier decomposition, a vortical-potential gust splitting analysis is implemented to determine the vortical and potential harmonic wake gust forcing functions both upstream and downstream of the rotor. Higher harmonics of the vortical gust component of the IGV wakes are found to decay at a uniform rate due to viscous diffusion.

  18. Aerosol simulation including chemical and nuclear reactions

    Energy Technology Data Exchange (ETDEWEB)

    Marwil, E.S.; Lemmon, E.C.

    1985-01-01

    The numerical simulation of aerosol transport, including the effects of chemical and nuclear reactions presents a challenging dynamic accounting problem. Particles of different sizes agglomerate and settle out due to various mechanisms, such as diffusion, diffusiophoresis, thermophoresis, gravitational settling, turbulent acceleration, and centrifugal acceleration. Particles also change size, due to the condensation and evaporation of materials on the particle. Heterogeneous chemical reactions occur at the interface between a particle and the suspending medium, or a surface and the gas in the aerosol. Homogeneous chemical reactions occur within the aersol suspending medium, within a particle, and on a surface. These reactions may include a phase change. Nuclear reactions occur in all locations. These spontaneous transmutations from one element form to another occur at greatly varying rates and may result in phase or chemical changes which complicate the accounting process. This paper presents an approach for inclusion of these effects on the transport of aerosols. The accounting system is very complex and results in a large set of stiff ordinary differential equations (ODEs). The techniques for numerical solution of these ODEs require special attention to achieve their solution in an efficient and affordable manner. 4 refs.

  19. Detailed requirements document for the Interactive Financial Management System (IFMS), volume 1

    Science.gov (United States)

    Dodson, D. B.

    1975-01-01

    The detailed requirements for phase 1 (online fund control, subauthorization accounting, and accounts receivable functional capabilities) of the Interactive Financial Management System (IFMS) are described. This includes information on the following: systems requirements, performance requirements, test requirements, and production implementation. Most of the work is centered on systems requirements, and includes discussions on the following processes: resources authority, allotment, primary work authorization, reimbursable order acceptance, purchase request, obligation, cost accrual, cost distribution, disbursement, subauthorization performance, travel, accounts receivable, payroll, property, edit table maintenance, end-of-year, backup input. Other subjects covered include: external systems interfaces, general inquiries, general report requirements, communication requirements, and miscellaneous. Subjects covered under performance requirements include: response time, processing volumes, system reliability, and accuracy. Under test requirements come test data sources, general test approach, and acceptance criteria. Under production implementation come data base establishment, operational stages, and operational requirements.

  20. Using VIS/NIR and IR spectral cameras for detecting and separating crime scene details

    Science.gov (United States)

    Kuula, Jaana; Pölönen, Ilkka; Puupponen, Hannu-Heikki; Selander, Tuomas; Reinikainen, Tapani; Kalenius, Tapani; Saari, Heikki

    2012-06-01

    Detecting invisible details and separating mixed evidence is critical for forensic inspection. If this can be done reliably and fast at the crime scene, irrelevant objects do not require further examination at the laboratory. This will speed up the inspection process and release resources for other critical tasks. This article reports on tests which have been carried out at the University of Jyväskylä in Finland together with the Central Finland Police Department and the National Bureau of Investigation for detecting and separating forensic details with hyperspectral technology. In the tests evidence was sought after at an assumed violent burglary scene with the use of VTT's 500-900 nm wavelength VNIR camera, Specim's 400- 1000 nm VNIR camera, and Specim's 1000-2500 nm SWIR camera. The tested details were dried blood on a ceramic plate, a stain of four types of mixed and absorbed blood, and blood which had been washed off a table. Other examined details included untreated latent fingerprints, gunshot residue, primer residue, and layered paint on small pieces of wood. All cameras could detect visible details and separate mixed paint. The SWIR camera could also separate four types of human and animal blood which were mixed in the same stain and absorbed into a fabric. None of the cameras could however detect primer residue, untreated latent fingerprints, or blood that had been washed off. The results are encouraging and indicate the need for further studies. The results also emphasize the importance of creating optimal imaging conditions into the crime scene for each kind of subjects and backgrounds.

  1. Synchronization of Green Algae by Light and Dark Regimes for Cell Cycle and Cell Division Studies.

    Science.gov (United States)

    Hlavová, Monika; Vítová, Milada; Bišová, Kateřina

    2016-01-01

    A synchronous population of cells is one of the prerequisites for studying cell cycle processes such as DNA replication, nuclear and cellular division. Green algae dividing by multiple fission represent a unique single cell system enabling the preparation of highly synchronous cultures by application of a light-dark regime similar to what they experience in nature. This chapter provides detailed protocols for synchronization of different algal species by alternating light-dark cycles; all critical points are discussed extensively. Moreover, detailed information on basic analysis of cell cycle progression in such cultures is presented, including analyses of nuclear, cellular, and chloroplast divisions. Modifications of basic protocols that enable changes in cell cycle progression are also suggested so that nuclear or chloroplast divisions can be followed separately.

  2. Strategies for replacement of obsolete equipment, including reverse engineering

    International Nuclear Information System (INIS)

    The presentation shall detail the challenges facing nuclear power plants with the replacement of obsolete equipment and the strategies used to overcome those challenges. The presentation will outline the common equipment types which are either obsolete or are becoming obsolete, with a focus on safety related components. The four options of the obsolete equipment replacement philosophy will be presented with replacement examples from each of the options shown for discussion purposes. Detailed examples from each of the four obsolete equipment replacement options of, (1) commercially available equivalent component, (2) modification of a commercial available component, (3) reverse engineering of the original component and finally (4) design changes using a new component, shall be presented to evaluate the advantages and disadvantages of each option. The presentation will include the technical challenges, cost and schedule concerns for each of the four options. Emphasis will be placed on the technological challenges associated with replacing old and obsolete equipment. The following is a bullet list of the challenges which will be discussed: 1) Missing, misleading or no information on the original component. 2) Acquiring information from the original equipment manufacturer and the plant. 3) Using a sample component for the replacement evaluation and or reverse engineering. 4) Reverse engineering old equipment with newly available discrete components. The presentation will include the equivalency documentation using the EPRI guidelines when replacing an original component with a different yet form, fit and functional equivalent component. The presentation will conclude with a discussion of the advantages and disadvantages of the replacement of the obsolete component with a form, fit and functional equivalent component vs. the replacement of the original component with a new component with today's technology. (author)

  3. Details and Consequences of Water Vapor Diffusion In The Pore Space of Snow

    Science.gov (United States)

    Sokratov, S. A.; Bartelt, P.; Schneebeli, M.; Lehning, M.

    Despite a long history of extensive experimental and theoretical studies on the process of water vapor diffusion in snow, no quantitative explanation for the observed diffu- sion characteristics such as mass-transfer rates and snow density change is available at present. Results of a detailed investigatation of the process are presented. The pro- posed description of water vapor flux in snow now includes thermal diffusion, grav- itation, convective air flow, and volumetric mass-production. The relative importance of the components in the overall mass-transfer is analyzed. Although experimental data of sufficient detail concerning the individual components are not available, the results of our analysis provide an improved understanding of the sources of discrepan- cies in published experimental results. The consequences of the water vapor transport description for heat transfer and metamorphism are also discussed.

  4. Detailed description and user`s manual of high burnup fuel analysis code EXBURN-I

    Energy Technology Data Exchange (ETDEWEB)

    Suzuki, Motoe [Japan Atomic Energy Research Inst., Tokai, Ibaraki (Japan). Tokai Research Establishment; Saitou, Hiroaki

    1997-11-01

    EXBURN-I has been developed for the analysis of LWR high burnup fuel behavior in normal operation and power transient conditions. In the high burnup region, phenomena occur which are different in quality from those expected for the extension of behaviors in the mid-burnup region. To analyze these phenomena, EXBURN-I has been formed by the incorporation of such new models as pellet thermal conductivity change, burnup-dependent FP gas release rate, and cladding oxide layer growth to the basic structure of low- and mid-burnup fuel analysis code FEMAXI-IV. The present report describes in detail the whole structure of the code, models, and materials properties. Also, it includes a detailed input manual and sample output, etc. (author). 55 refs.

  5. The Hybrid Detailed / Statistical Opacity Code SCO-RCG: New Developments and Applications

    CERN Document Server

    Pain, Jean-Christophe; Porcherot, Quentin; Blenski, Thomas

    2013-01-01

    We present the hybrid opacity code SCO-RCG which combines statistical approaches with fine-structure calculations. Radial integrals needed for the computation of detailed transition arrays are calculated by the code SCO (Super-configuration Code for Opacity), which calculates atomic structure at finite temperature and density, taking into account plasma effects on the wave-functions. Levels and spectral lines are then computed by an adapted RCG routine of R. D. Cowan. SCO-RCG now includes the Partially Resolved Transition Array model, which allows one to replace a complex transition array by a small-scale detailed calculation preserving energy and variance of the genuine transition array and yielding improved high-order moments. An approximate method for studying the impact of strong magnetic field on opacity and emissivity was also recently implemented.

  6. Direct numerical simulation of temporally evolving turbulent luminous jet flames with detailed fuel and soot chemistry

    Science.gov (United States)

    Lecoustre, Vivien; Arias, Paul; Roy, Somesh; Wang, Wei; Luo, Zhaoyu; Haworth, Dan; Im, Hong; Lu, Tianfeng; Ma, Kwan-Liu; Sankaran, Ramanan; Trouve, Arnaud

    2011-11-01

    Direct numerical simulations of 2D temporally-evolving luminous turbulent ethylene-air jet diffusion flames are performed using a high-order compressible Navier-Stokes solver. The simulations use a reduced mechanism derived from a detailed ethylene-air chemical kinetic mechanism that includes the reaction pathways for the formation of polycyclic aromatic hydrocarbons. The gas-phase chemistry is coupled with a detailed soot particle model based on the method of moments with interpolative closure that accounts for soot nucleation, coagulation, surface growth through HACA mechanism, and oxidation. Radiative heat transfer of CO2, H2O, and soot is treated by solving the radiative transfer equation using the discrete transfer method. This work presents preliminary results of radiation effects on soot dynamics at the tip of a jet diffusion flame with a particular focus on soot formation/oxidation.

  7. Direct numerical simulation of temporally evolving luminous jet flames with detailed fuel and soot chemistry

    Energy Technology Data Exchange (ETDEWEB)

    Sankaran, Ramanan [ORNL

    2011-01-01

    Direct numerical simulations of 2D temporally-evolving luminous turbulent ethylene-air jet diffusion flames are performed using a high-order compressible Navier-Stokes solver. The simulations use a reduced mechanism derived from a detailed ethylene-air chemical kinetic mechanism that includes the reaction pathways for the formation of polycyclic aromatic hydrocarbons. The gas-phase chemistry is coupled with a detailed soot particle model based on the method of moments with interpolative closure that accounts for soot nucleation, coagulation, surface growth through HACA mechanism, and oxidation. Radiative heat transfer of CO{sub 2}, H{sub 2}O, and soot is treated by solving the radiative transfer equation using the discrete transfer method. This work presents preliminary results of radiation effects on soot dynamics at the tip of a jet diffusion flame with a particular focus on soot formation/oxidation.

  8. Identification of a detailed function list for public health emergency management using three qualitative methods

    Institute of Scientific and Technical Information of China (English)

    HU Guo-qing; RAO Ke-qin; SUN Zhen-qiu

    2007-01-01

    Background At present, many administrators lack a theoretical guide to build management systemsto cope with public health emergencies. This study developed a detailed function list for public health emergency management to help building the management systems scientifically.Methods The five steps in this study including literature review, semistructured interviews with experts, focus group,Delphi study and focus group were conducted to develop the detailed list of functions.Results An initial list of 25 functions with 188 items was formed through literature review. The list was reduced to 10 functions with 57 items by semistructured interviews with experts and focus group. Next, all these 57 items reached the consensus through one-round Delphi study. Some revisions of the consensus were done by the same focus group.Conclusion The final 10 functions with 57 items could be used as a guide for most countries during their building of a public health emergency management system.

  9. A Catalog of Detailed Visual Morphological Classifications for 14034 Galaxies in the Sloan Digital Sky Survey

    CERN Document Server

    Nair, Preethi B

    2010-01-01

    We present a catalog of detailed visual classifications for 14034 galaxies in the Sloan Digital Sky Survey (SDSS) Data Release 4 (DR4). Our sample includes nearly all spectroscopically-targeted galaxies in the redshift range 0.01detail the systematics and biases of our sample and summarize the overall statistical properties of the sample, noting the most obvious trends that are relevant for general comparisons of our catalog with previously published work.

  10. Detailed simulations of lighting conditions in office rooms lit by daylight and artificial light

    DEFF Research Database (Denmark)

    Iversen, Anne

    . The detailed simulations includes studies of the resolution of different weather data sets in climate-based daylight modeling. Furthermore, influence of the electrical lighting demand by simulating with dynamic occupancy patterns is studied. Finally the thesis explores the influence of obstructions in an urban......In this thesis the effect on the annual artificial lighting demand is investigated by employing detailed simulations of lighting conditions in office rooms lit by daylight and artificial. The simulations of the artificial lighting demand is accomplished through daylight simulations in Radiance...... canyon on the daylight availability within the buildings, and hence on the energy consumption for artificial lights. The results from the thesis demonstrates that the effect on the outcome of the daylight simulations when simulating with typical weather data files for the location of Copenhagen...

  11. CosmoSpec: Fast and detailed computation of the cosmological recombination radiation from hydrogen and helium

    CERN Document Server

    Chluba, Jens

    2015-01-01

    We present the first fast and detailed computation of the cosmological recombination radiation released during the hydrogen (redshift z ~ 1300) and helium (z ~ 2500 and z ~ 6000) recombination epochs, introducing the code CosmoSpec. Our computations include important radiative transfer effects, 500-shell bound-bound and free-bound emission for all three species, the effects of electron scattering and free-free absorption as well as interspecies (HeII --> HeI --> HI) photon feedback. The latter effect modifies the shape and amplitude of the recombination radiation and CosmoSpec improves significantly over previous treatments of it. Utilizing effective multilevel atom and conductance approaches, one calculation takes only ~ 15 seconds on a standard laptop as opposed to days for previous computations. This is an important step towards detailed forecasts and feasibility studies considering the detection of the cosmological recombination lines and what one may hope to learn from the ~ 6.1 photons emitted per hydro...

  12. Models of bovine babesiosis including juvenile cattle.

    Science.gov (United States)

    Saad-Roy, C M; Shuai, Zhisheng; van den Driessche, P

    2015-03-01

    Bovine Babesiosis in cattle is caused by the transmission of protozoa of Babesia spp. by ticks as vectors. Juvenile cattle (resistance to Bovine Babesiosis, rarely show symptoms, and acquire immunity upon recovery. Susceptibility to the disease varies between breeds of cattle. Models of the dynamics of Bovine Babesiosis transmitted by the cattle tick that include these factors are formulated as systems of ordinary differential equations. Basic reproduction numbers are calculated, and it is proved that if these numbers are below the threshold value of one, then Bovine Babesiosis dies out. However, above the threshold number of one, the disease may approach an endemic state. In this case, control measures are suggested by determining target reproduction numbers. The percentage of a particular population (for example, the adult bovine population) needed to be controlled to eradicate the disease is evaluated numerically using Columbia data from the literature. PMID:25715822

  13. CLIC expands to include the Southern Hemisphere

    CERN Multimedia

    Roberto Cantoni

    2010-01-01

    Australia has recently joined the CLIC collaboration: the enlargement will bring new expertise and resources to the project, and is especially welcome in the wake of CERN budget redistributions following the recent adoption of the Medium Term Plan.   The countries involved in CLIC collaboration With the signing of a Memorandum of Understanding on 26 August 2010, the ACAS network (Australian Collaboration for Accelerator Science) became the 40th member of in the multilateral CLIC collaboration making Australia the 22nd country to join the collaboration. “The new MoU was signed by the ACAS network, which includes the Australian Synchrotron and the University of Melbourne”, explains Jean-Pierre Delahaye, CLIC Study Leader. “Thanks to their expertise, the Australian institutes will contribute greatly to the CLIC damping rings and the two-beam test modules." Institutes from any country wishing to join the CLIC collaboration are invited to assume responsibility o...

  14. CERN Technical Training: LABVIEW courses include RADE

    CERN Multimedia

    HR Department

    2009-01-01

    The contents of the "LabView Basic I" and "LabView Intermediate II" courses have recently been changed to include, respectively, an introduction to and expert training in the Rapid Application Development Environment (RADE). RADE is a LabView-based application developed at CERN to integrate LabView in the accelerator and experiment control infrastructure. It is a suitable solution to developing expert tools, machine development analysis and independent test facilities. The course names have also been changed to "LabVIEW Basics I with RADE Introduction" and "LabVIEW Intermediate II with Advanced RADE Application". " LabVIEW Basics I with RADE Introduction" is designed for: Users preparing to develop applications using LabVIEW, or NI Developer Suite; users and technical managers evaluating LabVIEW or NI Developer Suite in purchasing decisions; users pursuing the Certified LabVIEW Developer certification. The course pr...

  15. CERN Technical Training: LABVIEW courses include RADE

    CERN Multimedia

    HR Department

    2009-01-01

    The contents of "LabView Basic I" and "LabView Intermediate II" trainings have been recently changed to include, respectively, an introduction and an expert training on the Rapid Application Development Environment (RADE). RADE is a LabView-based application developed at CERN to integrate LabView in the accelerator and experiment control infrastructure. It is a suitable solution to develop expert tools, machine development analysis and independent test facilities. The course names have also been changed to "LabVIEW Basics I with RADE Introduction" and "LabVIEW Intermediate II with Advanced RADE Application". " LabVIEW Basics I with RADE Introduction" is designed for: Users preparing to develop applications using LabVIEW, or NI Developer Suite; users and technical managers evaluating LabVIEW or NI Developer Suite in purchasing decisions; users pursuing the Certified LabVIEW Developer certification. The course prepare...

  16. CERN Technical Training: LABVIEW courses include RADE

    CERN Multimedia

    HR Department

    2009-01-01

    The contents of the "LabView Basic I" and "LabView Intermediate II" courses have recently been changed to include, respectively, an introduction to and expert training in the Rapid Application Development Environment (RADE). RADE is a LabView-based application developed at CERN to integrate LabView in the accelerator and experiment control infrastructure. It is a suitable solution to developing expert tools, machine development analysis and independent test facilities. The course names have also been changed to "LabVIEW Basics I with RADE Introduction" and "LabVIEW Intermediate II with Advanced RADE Application". " LabVIEW Basics I with RADE Introduction" is designed for: Users preparing to develop applications using LabVIEW, or NI Developer Suite; users and technical managers evaluating LabVIEW or NI Developer Suite in purchasing decisions; users pursuing the Certified LabVIEW Developer certification. The course prepares participants to develop test and measurement, da...

  17. Critical point anomalies include expansion shock waves

    Energy Technology Data Exchange (ETDEWEB)

    Nannan, N. R., E-mail: ryan.nannan@uvs.edu [Mechanical Engineering Discipline, Anton de Kom University of Suriname, Leysweg 86, PO Box 9212, Paramaribo, Suriname and Process and Energy Department, Delft University of Technology, Leeghwaterstraat 44, 2628 CA Delft (Netherlands); Guardone, A., E-mail: alberto.guardone@polimi.it [Department of Aerospace Science and Technology, Politecnico di Milano, Via La Masa 34, 20156 Milano (Italy); Colonna, P., E-mail: p.colonna@tudelft.nl [Propulsion and Power, Delft University of Technology, Kluyverweg 1, 2629 HS Delft (Netherlands)

    2014-02-15

    From first-principle fluid dynamics, complemented by a rigorous state equation accounting for critical anomalies, we discovered that expansion shock waves may occur in the vicinity of the liquid-vapor critical point in the two-phase region. Due to universality of near-critical thermodynamics, the result is valid for any common pure fluid in which molecular interactions are only short-range, namely, for so-called 3-dimensional Ising-like systems, and under the assumption of thermodynamic equilibrium. In addition to rarefaction shock waves, diverse non-classical effects are admissible, including composite compressive shock-fan-shock waves, due to the change of sign of the fundamental derivative of gasdynamics.

  18. Including electronic effects in damage cascade simulations

    International Nuclear Information System (INIS)

    A method for including the effects of electronic losses and electron-phonon coupling in radiation damage simulations has been developed and implemented for 10 keV cascades in Fe. The MD simulations are coupled to a continuum model for the electronic energy and energy lost by the atoms, due to electronic friction and electron-phonon coupling, is gained by electronic system. Electronic energy transport is described by the heat diffusion equation and energy is returned to the lattice via a stochastic force. Thus the temperature of the atomic system is controlled by a Langevin thermostat at the local electronic temperature, which varies with time and space. The results of simulations with this inhomogeneous thermostat are compared with those of homogeneous (constant temperature) thermostat simulations for a range of electron-phonon coupling strengths. The residual defect concentration was found to have a non-monotonic variation with coupling strength.

  19. AMS at the ANU including biomedical applications

    Energy Technology Data Exchange (ETDEWEB)

    Fifield, L.K.; Allan, G.L.; Cresswell, R.G.; Ophel, T.R. [Australian National Univ., Canberra, ACT (Australia); King, S.J.; Day, J.P. [Manchester Univ. (United Kingdom). Dept. of Chemistry

    1993-12-31

    An extensive accelerator mass spectrometry program has been conducted on the 14UD accelerator at the Australian National University since 1986. In the two years since the previous conference, the research program has expanded significantly to include biomedical applications of {sup 26}Al and studies of landform evolution using isotopes produced in situ in surface rocks by cosmic ray bombardment. The system is now used for the measurement of {sup 10}Be, {sup 14}C, {sup 26}Al, {sup 36}Cl, {sup 59}Ni and {sup 129}I, and research is being undertaken in hydrology, environmental geochemistry, archaeology and biomedicine. On the technical side, a new test system has permitted the successful off-line development of a high-intensity ion source. A new injection line to the 14UD has been established and the new source is now in position and providing beams to the accelerator. 4 refs.

  20. Methods for derivation of multipotent neural crest cells derived from human pluripotent stem cells

    Science.gov (United States)

    Avery, John; Dalton, Stephen

    2016-01-01

    Summary Multipotent, neural crest cells (NCCs) produce a wide-range of cell types during embryonic development. This includes melanocytes, peripheral neurons, smooth muscle cells, osteocytes, chondrocytes and adipocytes. The protocol described here allows for highly-efficient differentiation of human pluripotent stem cells to a neural crest fate within 15 days. This is accomplished under feeder-free conditions, using chemically defined medium supplemented with two small molecule inhibitors that block glycogen synthase kinase 3 (GSK3) and bone morphogenic protein (BMP) signaling. This technology is well-suited as a platform to understand in greater detail the pathogenesis of human disease associated with impaired neural crest development/migration. PMID:25986498