WorldWideScience

Sample records for cell-surface electrical potential

  1. Ephemeral Electric Potential and Electric Field Sensor

    Science.gov (United States)

    Generazio, Edward R. (Inventor)

    2017-01-01

    Systems, methods, and devices of the various embodiments provide for the minimization of the effects of intrinsic and extrinsic leakage electrical currents enabling true measurements of electric potentials and electric fields. In an embodiment, an ephemeral electric potential and electric field sensor system may have at least one electric field sensor and a rotator coupled to the electric field sensor and be configured to rotate the electric field sensor at a quasi-static frequency. In an embodiment, ephemeral electric potential and electric field measurements may be taken by rotating at least one electric field sensor at a quasi-static frequency, receiving electrical potential measurements from the electric field sensor when the electric field sensor is rotating at the quasi-static frequency, and generating and outputting images based at least in part on the received electrical potential measurements.

  2. Graphing Electric Potential.

    Science.gov (United States)

    De Jong, Marvin L.

    1993-01-01

    Describes the powerful graphing ability of computer algebra systems (CAS) to create three-dimensional graphs or surface graphics of electric potentials. Provides equations along with examples of the printouts. Lists the programs Mathematica, Maple, Derive, Theorist, MathCad, and MATLAB as promising CAS systems. (MVL)

  3. Immunogenicity and diagnostic potential of synthetic antigenic cell surface glycans of Leishmania.

    Science.gov (United States)

    Anish, Chakkumkal; Martin, Christopher E; Wahlbrink, Annette; Bogdan, Christian; Ntais, Pantelis; Antoniou, Maria; Seeberger, Peter H

    2013-11-15

    Detection and quantification of pathogen-derived antigenic structures is a key method for the initial diagnosis and follow-up of various infectious diseases. Complex parasitic diseases such as leishmaniasis require highly sensitive and specific tests prior to treatment with potentially toxic drugs. To investigate the diagnostic potential of cell surface glycans found on Leishmania parasites, we identified diagnostically relevant glycan epitopes and used synthetic glycan microarrays to screen sera from infected humans and dogs. On the basis of the screening results, we selected a tetrasaccharide to generate anti-glycan antibodies. The corresponding tetrasaccharide-carrier protein conjugate was immunogenic in mice, and sera obtained from immunized mice specifically detected the Leishmania parasite. These results demonstrate how synthetic glycan arrays, in combination with immunological methods, help to identify promising carbohydrate antigens for pathogen detection.

  4. Electric potential and electric field imaging

    Science.gov (United States)

    Generazio, E. R.

    2017-02-01

    The technology and methods for remote quantitative imaging of electrostatic potentials and electrostatic fields in and around objects and in free space is presented. Electric field imaging (EFI) technology may be applied to characterize intrinsic or existing electric potentials and electric fields, or an externally generated electrostatic field made be used for "illuminating" volumes to be inspected with EFI. The baseline sensor technology (e-Sensor) and its construction, optional electric field generation (quasi-static generator), and current e-Sensor enhancements (ephemeral e-Sensor) are discussed. Demonstrations for structural, electronic, human, and memory applications are shown. This new EFI capability is demonstrated to reveal characterization of electric charge distribution creating a new field of study embracing areas of interest including electrostatic discharge (ESD) mitigation, crime scene forensics, design and materials selection for advanced sensors, dielectric morphology of structures, tether integrity, organic molecular memory, and medical diagnostic and treatment efficacy applications such as cardiac polarization wave propagation and electromyography imaging.

  5. Cell Surface Glycoprotein of Reactive Stromal Fibroblasts as a Potential Antibody Target in Human Epithelial Cancers

    Science.gov (United States)

    Garin-Chesa, Pilar; Old, Lloyd J.; Rettig, Wolfgang J.

    1990-09-01

    The F19 antigen is a cell surface glycoprotein (M_r, 95,000) of human sarcomas and proliferating, cultured fibroblasts that is absent from resting fibroblasts in normal adult tissues. Normal and malignant epithelial cells are also F19^-. The present immunohistochemical study describes induction of F19 in the reactive mesenchyme of epithelial tumors. F19^+ fibroblasts were found in primary and metastatic carcinomas, including colorectal (18 of 18 cases studied), breast (14/14), ovarian (21/21), bladder (9/10), and lung carcinomas (13/13). In contrast, the stroma of benign colorectal adenomas, fibrocystic disease and fibroadenomas of breast, benign prostate hyperplasia, in situ bladder carcinomas, and benign ovarian tumors showed no or only moderate numbers of F19^+ fibroblasts. Analysis of dermal incision wounds revealed that F19 is strongly induced during scar formation. Comparison of F19 with the extracellular matrix protein tenascin, a putative marker of tumor mesenchyme, showed a cellular staining pattern for F19 vs. the extracellular matrix pattern for tenascin and widespread expression of tenascin in F19^- normal tissues and benign tumors. Our results suggest that the F19^+ phenotype correlates with specialized fibroblast functions in wound healing and malignant tumor growth. Because of its abundance in tumor mesenchyme, F19 may serve as a target for antibodies labeled with radioisotopes or toxic agents, or inflammatogenic antibodies, in carcinoma patients.

  6. Characterization of the osteogenic potential of mesenchymal stem cells from human periodontal ligament based on cell surface markers.

    Science.gov (United States)

    Alvarez, Ruth; Lee, Hye-Lim; Wang, Cun-Yu; Hong, Christine

    2015-12-18

    Mesenchymal stem cell (MSC)-mediated therapy has been shown to be clinically effective in regenerating tissue defects. For improved regenerative therapy, it is critical to isolate homogenous populations of MSCs with high capacity to differentiate into appropriate tissues. The utilization of stem cell surface antigens provides a means to identify MSCs from various tissues. However, few surface markers that consistently isolate highly regenerative MSCs have been validated, making it challenging for routine clinical applications and making it all the more imperative to identify reliable surface markers. In this study, we used three surface marker combinations: CD51/CD140α, CD271, and STRO-1/CD146 for the isolation of homogenous populations of dental mesenchymal stem cells (DMSCs) from heterogeneous periodontal ligament cells (PDLCs). Fluorescence-activated cell sorting analysis revealed that 24% of PDLCs were CD51(+)/CD140α(+), 0.8% were CD271(+), and 2.4% were STRO-1(+)/CD146(+). Sorted cell populations were further assessed for their multipotent properties by inducing osteogenic and chondrogenic differentiation. All three subsets of isolated DMSCs exhibited differentiation capacity into osteogenic and chondrogenic lineages but with varying degrees. CD271(+) DMSCs demonstrated the greatest osteogenic potential with strong induction of osteogenic markers such as DLX5, RUNX2, and BGLAP. Our study provides evidence that surface marker combinations used in this study are sufficient markers for the isolation of DMSCs from PDLCs. These results provide important insight into using specific surface markers for identifying homogenous populations of DMSCs for their improved utilization in regenerative medicine.

  7. IMPLICATIONS OF MICROBIAL ADHESION TO HYDROCARBONS FOR EVALUATING CELL-SURFACE HYDROPHOBICITY .1. ZETA-POTENTIALS OF HYDROCARBON DROPLETS

    NARCIS (Netherlands)

    BUSSCHER, HJ; VANDEBELTGRITTER, B; VANDERMEI, HC

    1995-01-01

    Microbial adhesion to hydrocarbons (MATH) is generally considered to be a measure of the organisms cell surface hydrophobicity. As microbial adhesion is a complicated interplay of long-range van der Waals and electrostatic forces and various short-range interactions, the above statement only holds

  8. Nitrogen-deficient microalgae are rich in cell-surface mannose: potential implications for prey biorecognition by phagotrophic protozoa

    Science.gov (United States)

    Martel, Claire M.

    2009-01-01

    Flow cytometry was used to quantify the abundance of mannose-linked glycoconjugates on microalgae precultured using low- or high-nitrate media. Nitrogen-deficient microalgae were richer in cell-surface mannose than nitrogen-sufficient. Findings are discussed in view of recent research which reveals mannose-specific ‘feeding receptors’ assist prey biorecognition by phagotrophic protozoa that ingest microalgae. PMID:24031323

  9. TEACHING PHYSICS: Teaching electric current and electrical potential

    Science.gov (United States)

    Carlton, Kevin

    1999-11-01

    Electric current and electrical potential is an abstract topic that students find great difficulty in understanding. This paper presents a teaching strategy aimed at facilitating the learner in developing a useful mechanistic mental model of the nature of simple electric circuits. The model is not at the highest level required of a graduate physicist but should be appropriate to students reaching a good secondary education level.

  10. Improving tumor targeting and therapeutic potential of Salmonella VNP20009 by displaying cell surface CEA-specific antibodies.

    Science.gov (United States)

    Bereta, Michal; Hayhurst, Andrew; Gajda, Mariusz; Chorobik, Paulina; Targosz, Marta; Marcinkiewicz, Janusz; Kaufman, Howard L

    2007-05-22

    Genetically modified Salmonella typhimurium VNP20009 (VNP) is a useful vehicle for cancer therapy and vaccine development but exhibits limited tumor targeting in vivo. We engineered a novel VNP derivative that expressed carcinoembryonic antigen (CEA)-specific single chain antibody fragments (scFv) on the cell surface to increase tumor-specific targeting. There was significant scFv cell surface display visualized by flow cytometry and confocal microscopy when cells were probed with fluorescently labeled CEA. Atomic force microscopy (AFM) measurements on whole bacteria confirmed binding of unlabeled CEA to the displayed scFv. The modified VNP strain exhibited increased localization in the upper gastrointestinal tract of CEA transgenic mice and accumulated in CEA-expressing tumors. Furthermore, treatment with a single dose of the VNP derivative inhibited growth of MC38CEA tumors and was associated with local accumulation of CD3(+) T cells and CD11b(+) macrophages. The display of antibody fragments on the surface of VNP represents a novel strategy for both targeting CEA-expressing tumors and increasing the immunogenicity of Salmonella-based vaccines for cancer.

  11. Electric potential microelectrode for studies of electrobiogeophysics

    DEFF Research Database (Denmark)

    Damgaard, Lars Riis; Risgaard-Petersen, Nils; Nielsen, Lars Peter

    2014-01-01

    increase by approximately 2mV from the sediment-water interface to a depth of approximately 20mm, in accordance with the location and direction of the electric currents estimated from oxygen, pH, and H2S microprofiles. The EPM also captured emergence and decay of electric diffusion potentials......Spatially separated electron donors and acceptors in sediment can be exploited by the so-called “cable bacteria.” Electric potential microelectrodes (EPMs) were constructed to measure the electric fields that should appear when cable bacteria conduct electrons over centimeter distances. The EPMs...... were needle-shaped, shielded Ag/AgCl half-cells that were rendered insensitive to redox-active species in the environment. Tip diameters of 40 to 100 μm and signal resolution of approximately 10 μV were achieved. A test in marine sediments with active cable bacteria showed an electric potential...

  12. Electric Potential and Electric Field Imaging with Applications

    Science.gov (United States)

    Generazio, Ed

    2016-01-01

    The technology and techniques for remote quantitative imaging of electrostatic potentials and electrostatic fields in and around objects and in free space is presented. Electric field imaging (EFI) technology may be applied to characterize intrinsic or existing electric potentials and electric fields, or an externally generated electrostatic field may be used for (illuminating) volumes to be inspected with EFI. The baseline sensor technology, electric field sensor (e-sensor), and its construction, optional electric field generation (quasistatic generator), and current e-sensor enhancements (ephemeral e-sensor) are discussed. Demonstrations for structural, electronic, human, and memory applications are shown. This new EFI capability is demonstrated to reveal characterization of electric charge distribution, creating a new field of study that embraces areas of interest including electrostatic discharge mitigation, crime scene forensics, design and materials selection for advanced sensors, dielectric morphology of structures, inspection of containers, inspection for hidden objects, tether integrity, organic molecular memory, and medical diagnostic and treatment efficacy applications such as cardiac polarization wave propagation and electromyography imaging.

  13. Electric Potential and Electric Field Imaging with Dynamic Applications & Extensions

    Science.gov (United States)

    Generazio, Ed

    2017-01-01

    The technology and methods for remote quantitative imaging of electrostatic potentials and electrostatic fields in and around objects and in free space is presented. Electric field imaging (EFI) technology may be applied to characterize intrinsic or existing electric potentials and electric fields, or an externally generated electrostatic field made be used for volumes to be inspected with EFI. The baseline sensor technology (e-Sensor) and its construction, optional electric field generation (quasi-static generator), and current e- Sensor enhancements (ephemeral e-Sensor) are discussed. Critical design elements of current linear and real-time two-dimensional (2D) measurement systems are highlighted, and the development of a three dimensional (3D) EFI system is presented. Demonstrations for structural, electronic, human, and memory applications are shown. Recent work demonstrates that phonons may be used to create and annihilate electric dipoles within structures. Phonon induced dipoles are ephemeral and their polarization, strength, and location may be quantitatively characterized by EFI providing a new subsurface Phonon-EFI imaging technology. Results from real-time imaging of combustion and ion flow, and their measurement complications, will be discussed. Extensions to environment, Space and subterranean applications will be presented, and initial results for quantitative characterizing material properties are shown. A wearable EFI system has been developed by using fundamental EFI concepts. These new EFI capabilities are demonstrated to characterize electric charge distribution creating a new field of study embracing areas of interest including electrostatic discharge (ESD) mitigation, manufacturing quality control, crime scene forensics, design and materials selection for advanced sensors, combustion science, on-orbit space potential, container inspection, remote characterization of electronic circuits and level of activation, dielectric morphology of

  14. Consumers and green electricity : profiling potential purchasers

    Energy Technology Data Exchange (ETDEWEB)

    Rowlands, I. [Waterloo Univ., ON (Canada). Dept. of Environment and Resource Studies; Scott, D. [Environment Canada, Downsview, ON (Canada). Adaptation and Impacts Research Group; Parker, P. [Waterloo Univ., ON (Canada). Dept. of Geography

    2001-07-01

    Electricity markets around the world are being opened to competition and environmental concerns are prompting consumers to consider buying electricity that has been generated by renewable energy sources such as solar or wind power. This report profiles the potential purchaser of such green electricity. It presents 3 sets of hypotheses, each drawing upon the literature on green product purchasers. It is suggested that those who are willing to pay higher premiums for green electricity are likely to have certain demographic characteristics and social values. This study is based on a case-study of a survey conducted in the Waterloo region of southern Ontario, Canada, the objective of which was to determine what kind of people are potential green electricity purchasers. Results were presented to power managers and marketers. A 158-item survey was sent to 1,110 individuals, between September 2000 and April 2001. A total of 474 questionnaires were answered, for a response rate of 43 per cent. It was noted that although the response rate was high, the survey may not be representative because the respondents were older, better educated, and wealthier than the general population. In addition, the respondents had previously indicated they were willing to pay $25 for a home energy evaluation, suggesting a special interest in issues regarding energy and the environment. It was determined that attitudinal characteristics, such as liberalism and perceived consumer effectiveness, ideally identify the potential green electricity buyer. 34 refs., 5 tabs.

  15. Global potential for wind-generated electricity

    Science.gov (United States)

    Lu, Xi; McElroy, Michael B.; Kiviluoma, Juha

    2009-01-01

    The potential of wind power as a global source of electricity is assessed by using winds derived through assimilation of data from a variety of meteorological sources. The analysis indicates that a network of land-based 2.5-megawatt (MW) turbines restricted to nonforested, ice-free, nonurban areas operating at as little as 20% of their rated capacity could supply >40 times current worldwide consumption of electricity, >5 times total global use of energy in all forms. Resources in the contiguous United States, specifically in the central plain states, could accommodate as much as 16 times total current demand for electricity in the United States. Estimates are given also for quantities of electricity that could be obtained by using a network of 3.6-MW turbines deployed in ocean waters with depths <200 m within 50 nautical miles (92.6 km) of closest coastlines. PMID:19549865

  16. 30 CFR 57.12011 - High-potential electrical conductors.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false High-potential electrical conductors. 57.12011... Electricity Surface and Underground § 57.12011 High-potential electrical conductors. High-potential electrical conductors shall be covered, insulated, or placed to prevent contact with low potential conductors. ...

  17. Electrically tunable artificial gauge potential for polaritons

    Science.gov (United States)

    Lim, Hyang-Tag; Togan, Emre; Kroner, Martin; Miguel-Sanchez, Javier; Imamoğlu, Atac

    2017-02-01

    Neutral particles subject to artificial gauge potentials can behave as charged particles in magnetic fields. This fascinating premise has led to demonstrations of one-way waveguides, topologically protected edge states and Landau levels for photons. In ultracold neutral atoms, effective gauge fields have allowed the emulation of matter under strong magnetic fields leading to realization of Harper-Hofstadter and Haldane models. Here we show that application of perpendicular electric and magnetic fields effects a tunable artificial gauge potential for two-dimensional microcavity exciton polaritons. For verification, we perform interferometric measurements of the associated phase accumulated during coherent polariton transport. Since the gauge potential originates from the magnetoelectric Stark effect, it can be realized for photons strongly coupled to excitations in any polarizable medium. Together with strong polariton-polariton interactions and engineered polariton lattices, artificial gauge fields could play a key role in investigation of non-equilibrium dynamics of strongly correlated photons.

  18. Electric Potential Distribution in an Electrochemical Cell

    Science.gov (United States)

    Millet, Pierre

    1996-10-01

    Many students learning electrochemistry do not understand how current flows from one electrode to the other in an electrochemical cell. This is generally because interfacial phenomena and ionic conductivity in the electrolytic solution tend to be treated separately by chemistry teachers. An easy way to improve students' understanding of "how it works" is to compute the two dimensional electric potential distribution in the cell and to make a gravitational analogy. Current flows from one electrode to the other just like a ball would do in a gravitational field.

  19. 30 CFR 56.12011 - High-potential electrical conductors.

    Science.gov (United States)

    2010-07-01

    ... 30 Mineral Resources 1 2010-07-01 2010-07-01 false High-potential electrical conductors. 56.12011... § 56.12011 High-potential electrical conductors. High-potential electrical conductors shall be covered, insulated, or placed to prevent contact with low potential conductors. ...

  20. Predicting the Potential Market for Electric Vehicles

    DEFF Research Database (Denmark)

    Jensen, Anders Fjendbo; Cherchi, Elisabetta; Mabit, Stefan Lindhard

    2017-01-01

    Forecasting the potential demand for electric vehicles is a challenging task. Because most studies for new technologies rely on stated preference (SP) data, market share predictions will reflect shares in the SP data and not in the real market. Moreover, typical disaggregate demand models are sui....... Accounting for the diffusion effect, instead allows predicting the usual slow penetration of a new product in the initial years after product launch and a faster market share increase after diffusion takes place.......Forecasting the potential demand for electric vehicles is a challenging task. Because most studies for new technologies rely on stated preference (SP) data, market share predictions will reflect shares in the SP data and not in the real market. Moreover, typical disaggregate demand models...... are suitable to forecast demand in relatively stable markets, but show limitations in the case of innovations. When predicting the market for new products it is crucial to account for the role played by innovation and how it penetrates the new market over time through a diffusion process. However, typical...

  1. A Novel Platform for the Potentiation of Therapeutic Antibodies Based on Antigen-Dependent Formation of IgG Hexamers at the Cell Surface.

    Directory of Open Access Journals (Sweden)

    Rob N de Jong

    2016-01-01

    Full Text Available IgG antibodies can organize into ordered hexamers on cell surfaces after binding their antigen. These hexamers bind the first component of complement C1 inducing complement-dependent target cell killing. Here, we translated this natural concept into a novel technology platform (HexaBody technology for therapeutic antibody potentiation. We identified mutations that enhanced hexamer formation and complement activation by IgG1 antibodies against a range of targets on cells from hematological and solid tumor indications. IgG1 backbones with preferred mutations E345K or E430G conveyed a strong ability to induce conditional complement-dependent cytotoxicity (CDC of cell lines and chronic lymphocytic leukemia (CLL patient tumor cells, while retaining regular pharmacokinetics and biopharmaceutical developability. Both mutations potently enhanced CDC- and antibody-dependent cellular cytotoxicity (ADCC of a type II CD20 antibody that was ineffective in complement activation, while retaining its ability to induce apoptosis. The identified IgG1 Fc backbones provide a novel platform for the generation of therapeutics with enhanced effector functions that only become activated upon binding to target cell-expressed antigen.

  2. Electric breakdown potentials under longitudinal magnetic fields

    Energy Technology Data Exchange (ETDEWEB)

    Delgado-Aparicio V, L.F.; Soberon V P, F. [Pontificia Universidad Catolica del Peru, Lima (Peru). Seccion Fisica. Grupo de Investigacion en Plasmas. E-mail: plasma@pucp.edu.pe

    1998-07-01

    A study of a DC ionization potential with longitudinal magnetic fields in a parallel plate configuration is presented. A variation of the well known Paschen curve is studied for two different separation distances (2.0 and 6.7 cm) between the electrodes; more than orders of magnitude in pressures (1.4 x 10{sup -2} to 40 mbar); and magnetic fields up to 250 Gauss. The differences between the curves with and without B field are explained by the fluid model only by means of perpendicular mobility ({mu}) and diffusion (D) coefficients, cyclotron frequencies ({omega}{sub c}), Larmor radii (r-L) and collision frequencies v{sup =}{pi}{sup -1} with neutrals, independently of whether they produce ionization or not. Some inversions or crossings of the electric behavior between the right and left branch of different modified Paschen curves are due to the increasing collision frequencies and anomalous coefficients producing lower ionization potentials than the established ones in the absence of magnetic fields. (author)

  3. Lunar electric fields, surface potential and associated plasma sheaths

    Science.gov (United States)

    Freeman, J. W.; Ibrahim, M.

    1975-01-01

    A review is given of studies of the electric-field environment of the moon. Surface electric potentials are reported for the dayside and terminator regions, electron and ion densities in the plasma sheath adjacent to each surface-potential regime are evaluated, and the corresponding Debye lengths are estimated. The electric fields, which are approximated by the surface potential over the Debye length, are shown to be at least three orders of magnitude higher than the pervasive solar-wind electric field and to be confined to within a few tens of meters of the lunar surface.

  4. The potential for matrix conversion in marine electric propulsion systems.

    OpenAIRE

    Ciaramella, K. M.

    2006-01-01

    The growing interest in marine electric propulsion is driven by the need to improve ship performance. This thesis presents a review of existing marine electric propulsion technology and potential future variable speed drives. The matrix converter was found to be a promising alternative to existing technology and this thesis describes an investigation into its potential for marine electric propulsion. The matrix converter performs direct AC-AC conversion using a high switching frequency to pro...

  5. Action potential propagation: ion current or intramembrane electric field?

    Science.gov (United States)

    Martí, Albert; Pérez, Juan J; Madrenas, Jordi

    2018-01-01

    The established action potential propagation mechanisms do not satisfactorily explain propagation on myelinated axons given the current knowledge of biological channels and membranes. The flow across ion channels presents two possible effects: the electric potential variations across the lipid bilayers (action potential) and the propagation of an electric field through the membrane inner part. The proposed mechanism is based on intra-membrane electric field propagation, this propagation can explain the action potential saltatory propagation and its constant delay independent of distance between Ranvier nodes in myelinated axons.

  6. Potential demand and cost-benefit analysis of electric cars

    OpenAIRE

    Zito, Pietro; Salerno, Silvia

    2004-01-01

    In this study an analysis of electric family car performances is carried out. In particular, the aim of this research is to appraise the possibility of introducing electric cars in urban mobility and the evaluation of its economic feasibility. First of all, we determined the potential electric car demand, which was forecasted using a stated preference (SP) analysis. The survey was carried out at the University of Palermo considering a particular target of consumer: ‘the hybrid household’. A l...

  7. Multipurpose Electric Potential Sensor for Spacecraft Applications Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This proposal is based on a new, compact, solid-state electric potential sensor that has over an order of magnitude lower voltage noise than the prior...

  8. Defining Electric Potential Difference by Moving a Multimeter's Ground Probe

    Science.gov (United States)

    Stoeckel, Marta R.

    2018-01-01

    The abstract nature of electric potential difference (voltage) can make it a difficult concept to grasp, but understanding the relative nature of voltage is central to developing a conceptual understanding of electric circuits. In laboratory situations, I see these conceptual difficulties manifest when students have difficulty placing voltmeter probes appropriately or struggle to interpret unexpected multimeter readings, such as negative voltages. I developed this lab activity to reinforce the relative nature of electric potential difference and to provide students with a concrete understanding of what a voltmeter measures, including the significance of the placement of each multimeter probe.

  9. Potential Uses of Bagasse for Ethanol Production Versus Electricity Production

    Directory of Open Access Journals (Sweden)

    Zumalacárregui-De Cárdenas Lourdes Margarita

    2015-07-01

    Full Text Available The procedure to carry out the energy balance for ethanol production by bagasse’s hydrolysis is presented. The loss of potentialities for electric power generation when bagasse is used to produce ethanol instead of electricity directly is calculated. Potential losses are 45-64% according to the efficiency of the lignocellulosic ethanol production. The relationship that exists between the volume of ethanol and the efficiency of Otto and Rankine cycles is analyzed. Those cycles are used to produce electricity from ethanol and bagasse, respectively.

  10. Synthesis and in vitro anti-tumor activity of novel HPMA copolymer-drug conjugates with potential cell surface targeting property for carcinoma cells.

    Science.gov (United States)

    Xiang, Qingyu; Yang, Yang; Zhou, Zhou; Zhou, Dan; Jin, Yun; Zhang, Zhirong; Huang, Yuan

    2012-02-01

    In several groups of malignant tumors including head and neck tumors, a protein named Hsp47/CBP2 leaked from the cell was expressed on the tumor cell surface. Several synthetic peptides have been identified as effective ligands for binding to Hsp47/CBP2. This study has focused on the synthesis and in vitro characterization of a targeting delivery system of 5-fluorouracil (5-FU) to human head and neck squamous cell carcinoma (HNSCC) in order to improve anti-cancer efficacy and reduce dose-limiting toxicity of 5-FU. An N-(2-hydroxypropyl) methacrylamide (HPMA) copolymer, with Hsp47/CBP2 binding peptide sequence (namely WHYPWFQNWAMA) as a targeting ligand, was synthesized by a novel and simplified synthetic route. Under the controlled synthetic conditions, 1,3-dimethylol-5-FU, derived from 5-FU, was attached to the HPMA copolymer backbone via the lysosomally degradable GFLG linker, while the WHYPWFQNWAMA was conjugated via a non-degradable Gly-Gly (GG) linker. A control polymer without targeting moiety was also synthesized (P-FU). The in vitro cytotoxicity, internalization and apoptosis assays of the polymeric conjugates were evaluated. The characteristic apoptotic morphological changes were also assessed. Compared to 5-FU and P-FU, the HPMA copolymer containing the Hsp47/CBP2 binding peptide (P-FU-peptide) exhibited the highest cytotoxic efficacy to cell line of human head and neck squamous cell carcinoma (pHPMA copolymer-Hsp47/CBP2 binding peptide conjugates showed a promising future for the treatment of HNSCC with improved efficacy. Copyright © 2011 Elsevier B.V. All rights reserved.

  11. Understanding the Electrical Behavior of the Action Potential in Terms of Elementary Electrical Sources

    Science.gov (United States)

    Rodriguez-Falces, Javier

    2015-01-01

    A concept of major importance in human electrophysiology studies is the process by which activation of an excitable cell results in a rapid rise and fall of the electrical membrane potential, the so-called action potential. Hodgkin and Huxley proposed a model to explain the ionic mechanisms underlying the formation of action potentials. However,…

  12. Potential of Biomass Based Electricity Generation in Sri Lanka

    Directory of Open Access Journals (Sweden)

    KP Ariyadasa

    2015-12-01

    Full Text Available Biomass has attracted much attention as a primary energy source for electricity generation due to its potential to supply low cost fuel source with considerable environmental and socio-economic benefits. Despite having favorable climatic conditions to grow and use biomass for electricity generation, biomass based electricity generation in Sri Lanka is lagging behind due to many reasons. Many countries rely on the agricultural or forestry by-products or residuals as the main source of biomass for electricity generation mainly due to the comparatively low cost and sustainable supply of these by-products. Sri Lanka does not have this advantage and has to rely mainly on purposely grown biomass for electricity generation. Development of short rotation energy plantations seems to be the best option available for Sri Lanka to produce biomass for commercial scale electricity generation. The highly favorable growing conditions, availability of promising tree species and a variety of plantation management options and significant environmental and socio-economic benefits associated with energy plantation development greatly favor this option. This paper examines the potential of using plantation grown biomass as a fuel source for electricity generation in Sri Lanka.

  13. Detecting rapid mass movements using electrical self-potential measurements

    Science.gov (United States)

    Heinze, Thomas; Limbrock, Jonas; Pudasaini, Shiva P.; Kemna, Andreas

    2017-04-01

    Rapid mass movements are a latent danger for lives and infrastructure in almost any part of the world. Often such mass movements are caused by increasing pore pressure, for example, landslides after heavy rainfall or dam breaking after intrusion of water in the dam. Among several other geophysical methods used to observe water movement, the electrical self-potential method has been applied to a broad range of monitoring studies, especially focusing on volcanism and dam leakage but also during hydraulic fracturing and for earthquake prediction. Electrical self-potential signals may be caused by various mechanisms. Though, the most relevant source of the self-potential field in the given context is the streaming potential, caused by a flowing electrolyte through porous media with electrically charged internal surfaces. So far, existing models focus on monitoring water flow in non-deformable porous media. However, as the self-potential is sensitive to hydraulic parameters of the soil, any change in these parameters will cause an alteration of the electric signal. Mass movement will significantly influence the hydraulic parameters of the solid as well as the pressure field, assuming that fluid movement is faster than the pressure diffusion. We will present results of laboratory experiments under drained and undrained conditions with fluid triggered as well as manually triggered mass movements, monitored with self-potential measurements. For the undrained scenarios, we observe a clear correlation between the mass movements and signals in the electric potential, which clearly differ from the underlying potential variations due to increased saturation and fluid flow. In the drained experiments, we do not observe any measurable change in the electric potential. We therefore assume that change in fluid properties and release of the load causes disturbances in flow and streaming potential. We will discuss results of numerical simulations reproducing the observed effect. Our

  14. The Electrically Evoked Compound Action Potential: From Laboratory to Clinic.

    Science.gov (United States)

    He, Shuman; Teagle, Holly F B; Buchman, Craig A

    2017-01-01

    The electrically evoked compound action potential (eCAP) represents the synchronous firing of a population of electrically stimulated auditory nerve fibers. It can be directly recorded on a surgically exposed nerve trunk in animals or from an intra-cochlear electrode of a cochlear implant. In the past two decades, the eCAP has been widely recorded in both animals and clinical patient populations using different testing paradigms. This paper provides an overview of recording methodologies and response characteristics of the eCAP, as well as its potential applications in research and clinical situations. Relevant studies are reviewed and implications for clinicians are discussed.

  15. Bacterial Cell Surface Adsorption of Rare Earth Elements

    Science.gov (United States)

    Jiao, Y.; Park, D.; Reed, D.; Fujita, Y.; Yung, M.; Anderko, A.; Eslamimanesh, A.

    2015-12-01

    Rare earth elements (REE) play a critical role in many emerging clean energy technologies, including high-power magnets, wind turbines, solar panels, hybrid/electric vehicle batteries and lamp phosphors. In order to sustain demand for such technologies given current domestic REE shortages, there is a need to develop new approaches for ore processing/refining and recycling of REE-containing materials. To this end, we have developed a microbially-mediated bioadsorption strategy with application towards enrichment of REE from complex mixtures. Specifically, the bacterium Caulobacter crescentus was genetically engineered to display lanthanide binding tags (LBTs), short peptides that possess high affinity and specificity for rare earth elements, on its cell surface S-layer protein. Under optimal conditions, LBT-displayed cells adsorbed greater than 5-fold more REE than control cells lacking LBTs. Competition binding experiments with a selection of REEs demonstrated that our engineered cells could facilitate separation of light- from heavy- REE. Importantly, binding of REE onto our engineered strains was much more favorable compared to non-REE metals. Finally, REE bound to the cell surface could be stripped off using citrate, providing an effective and non-toxic REE recovery method. Together, this data highlights the potential of our approach for selective REE enrichment from REE containing mixtures.

  16. Geo-Electrical Investigation for Groundwater Potential of Ihievbe ...

    African Journals Online (AJOL)

    ADOWIE PERE

    2017-12-10

    Dec 10, 2017 ... ABSTRACT: This study was carried out within Ihiebe Ogben community in Akoko Edo LGA, Nigeria to investigate the ground water potential of the Basement Complex rocks using Vertical Electrical Sounding (VES) that deploys Schlumberger Array configuration. Evaluation of hydrogeological parameters ...

  17. Geo-electrical investigation for groundwater potential of Ihievbe ...

    African Journals Online (AJOL)

    This study was carried out within Ihiebe Ogben community in Akoko Edo LGA, Nigeria to investigate the ground water potential of the Basement Complex rocks using Vertical Electrical Sounding (VES) that deploys Schlumberger Array configuration. Evaluation of hydrogeological parameters shows fractured/ weathered ...

  18. Vertical electrical sounding to delineate the potential aquifer zones ...

    Indian Academy of Sciences (India)

    The delineation of potential aquifer zones is an important aspect for groundwater prospecting. Hence, the direct current (DC) resistivity soundings method also known as vertical electrical sounding (VES) is one of the most applied geophysical techniques for groundwater prospecting that was used in the capital city, Niamey ...

  19. Vertical Electrical Sounding to delineate the potential Aquifer zones ...

    Indian Academy of Sciences (India)

    48

    Vertical Electrical Sounding to delineate the potential Aquifer zones for drinking water in. Niamey city, Niger, Africa. Joy Choudhury*, LohithKumar K, E. Nagaiah, S Sonkamble, Shakeel Ahmed, Venay Kumar. Corresponding author email- joy8099@gmail.com. CSIR - National Geophysical Research Institute,. Uppal Road ...

  20. Equal-potential interpretation of electrically induced resonances in metamaterials

    DEFF Research Database (Denmark)

    Peng, Liang; Mortensen, N. Asger

    2011-01-01

    We propose a general description of electrically induced resonances (EIR) in metamaterials (MMs) comprising subwavelength unit cells. Based on classical electrodynamics, we found that EIR is governed by an equal-potential effect. Our theory accounts for the EIR phenomena and can give a renewed...... definition of the effective electric field and hence effective permittivity for MMs made of either dielectrics or metals as well as combinations thereof. The EIR, inherent to the periodic structures, may be the unifying origin of recently observed anomalous electromagnetic phenomena, e.g. the enhanced...... transmission, the suppressed transmission and the enhanced absorption by a variety of metal film structures in the terahertz range....

  1. Substantial improvements of fuel economy. Potentials of electric and hybrid electric vehicles

    Energy Technology Data Exchange (ETDEWEB)

    Joergensen, K. [Technical Univ. of Denmark (Denmark); Nielsen, L.H. [Forskningscenter Risoe (Denmark)

    1996-12-01

    This paper evaluates the scope for improvement of the energy and environmental impacts of road traffic by means of electrical and hybrid electric propulsion. These technologies promise considerable improvements of the fuel economy of vehicles compared to the present vehicle types as well as beneficial effects for the energy and traffic system. The paper - based on work carried out in the project `Transportation fuel based on renewable energy`, funded by the National Energy Agency of Denmark and carried out by Department of Buildings and Energy, Technical University of Denmark and System Analysis Department, Risoe National Laboratory - assesses the potentials for reduction of the primary energy consumption and emissions, and points to the necessary technical development to reap these benefits. A case study concerning passenger cars is analysed by means of computer simulations, comparing electric and hybrid electric passenger car to an equivalent reference vehicle (a conventional gasoline passenger car). (au) 10 refs.

  2. A Novel Platform for the Potentiation of Therapeutic Antibodies Based on Antigen-Dependent Formation of IgG Hexamers at the Cell Surface

    DEFF Research Database (Denmark)

    de Jong, R. N.; Beurskens, F. J.; Verploegen, S.

    2016-01-01

    ) for therapeutic antibody potentiation. We identified mutations that enhanced hexamer formation and complement activation by IgG1 antibodies against a range of targets on cells from hematological and solid tumor indications. IgG1 backbones with preferred mutations E345K or E430G conveyed a strong ability to induce...... conditional complement-dependent cytotoxicity (CDC) of cell lines and chronic lymphocytic leukemia (CLL) patient tumor cells, while retaining regular pharmacokinetics and biopharmaceutical developability. Both mutations potently enhanced CDC- and antibody-dependent cellular cytotoxicity (ADCC) of a type II CD...

  3. Small solar thermal electric power plants with early commercial potential

    Science.gov (United States)

    Jones, H. E.; Bisantz, D. J.; Clayton, R. N.; Heiges, H. H.; Ku, A. C.

    1979-01-01

    Cost-effective small solar thermal electric power plants (1- to 10-MW nominal size) offer an attractive way of helping the world meet its future energy needs. The paper describes the characteristics of a conceptual near-term plant (about 1 MW) and a potential 1990 commercial version. The basic system concept is one in which steam is generated using two-axis tracking, parabolic dish, and point-focusing collectors. The steam is transported through low-loss piping to a central steam turbine generator unit where it is converted to electricity. The plants have no energy storage and their output power level varies with the solar insolation level. This system concept, which is firmly based on state-of-the-art technology, is projected to offer one of the fastest paths for U.S. commercialization of solar thermal electric power plants through moderate technology advances and mass production.

  4. CPM Is a Useful Cell Surface Marker to Isolate Expandable Bi-Potential Liver Progenitor Cells Derived from Human iPS Cells

    Directory of Open Access Journals (Sweden)

    Taketomo Kido

    2015-10-01

    Full Text Available To develop a culture system for large-scale production of mature hepatocytes, liver progenitor cells (LPCs with a high proliferation potential would be advantageous. We have found that carboxypeptidase M (CPM is highly expressed in embryonic LPCs, hepatoblasts, while its expression is decreased along with hepatic maturation. Consistently, CPM expression was transiently induced during hepatic specification from human-induced pluripotent stem cells (hiPSCs. CPM+ cells isolated from differentiated hiPSCs at the immature hepatocyte stage proliferated extensively in vitro and expressed a set of genes that were typical of hepatoblasts. Moreover, the CPM+ cells exhibited a mature hepatocyte phenotype after induction of hepatic maturation and also underwent cholangiocytic differentiation in a three-dimensional culture system. These results indicated that hiPSC-derived CPM+ cells share the characteristics of LPCs, with the potential to proliferate and differentiate bi-directionally. Thus, CPM is a useful marker for isolating hiPSC-derived LPCs, which allows development of a large-scale culture system for producing hepatocytes and cholangiocytes.

  5. Comprehensive Screening of Cell Surface Markers Expressed by Adult-Derived Human Liver Stem/Progenitor Cells Harvested at Passage 5: Potential Implications for Engraftment

    Directory of Open Access Journals (Sweden)

    Pierre-Edouard Dollet

    2016-01-01

    Full Text Available Mesenchymal stromal cells (MSCs are known to have potential therapeutic benefits for a number of diseases. However, many studies report low engraftment levels, regardless of the target organ. One possible explanation could be that MSCs do not express the necessary receptors for engraftment. Indeed, MSCs appear to use a similar mechanism to leukocytes to engraft into injured organs, relying on various receptors for rolling, firm adhesion, and transmigration. In this study, we conducted an extensive surface molecule screening of adult-derived human liver stem/progenitor cells (ADHLSC in an attempt to shed some light on this subject. We observed that ADHLSCs lack expression of most of the costimulatory molecules tested. Furthermore, study of the adhesion molecule profile of ADHLSCs revealed that they do not express selectin ligands or LFA-1 which are, respectively, involved in the rolling process and the firm adhesion. In addition, ADHLSCs slightly express VLA-4 and lose expression of CXCR4 altogether on their surface during culture expansion. However, ADHLSCs express all the integrin couples and matrix metalloproteinases needed to bind and integrate the extracellular matrix once the endothelial barrier is crossed. Collectively, these results suggest that binding to the endothelium may be the critical weak point in the engraftment process.

  6. Early PREdiction of Severe Sepsis (ExPRES-Sepsis) study: protocol for an observational derivation study to discover potential leucocyte cell surface biomarkers.

    Science.gov (United States)

    Datta, Deepankar; Conway Morris, Andrew; Antonelli, Jean; Warner, Noel; Brown, Kenneth Alun; Wright, John; Simpson, A John; Rennie, Jillian; Hulme, Gillian; Lewis, Sion Marc; Mare, Tracey Anne; Cookson, Sharon; Weir, Christopher John; Dimmick, Ian; Keenan, Jim; Rossi, Adriano Giorgio; Shankar-Hari, Manu; Walsh, Timothy S

    2016-08-01

    Sepsis is an acute illness resulting from infection and the host immune response. Early identification of individuals at risk of developing life-threatening severe sepsis could enable early triage and treatment, and improve outcomes. Currently available biomarkers have poor predictive value for predicting subsequent clinical course in patients with suspected infection. Circulating leucocytes provide readily accessible tissues that reflect many aspects of the complex immune responses described in sepsis. We hypothesise that measuring cellular markers of immune responses by flow cytometry will enable early identification of infected patients at risk of adverse outcomes. We aim to characterise leucocyte surface markers (biomarkers) and their abnormalities in a population of patients presenting to the hospital emergency department with suspected sepsis, and explore their ability to predict subsequent clinical course. We will conduct a prospective, multicentre, clinical, exploratory, cohort observational study. To answer our study question, 3 patient populations will be studied. First, patients with suspected sepsis from the emergency department (n=300). To assess performance characteristics of potential tests, critically ill patients with established sepsis, and age and gender matched patients without suspicion of infection requiring hospital admission (both n=100) will be recruited as comparator populations. In all 3 groups, we plan to assess circulating biomarker profiles using flow cytometry. We will select candidate biomarkers by cross-cohort comparison, and then explore their predictive value for clinical outcomes within the cohort with suspected sepsis. The study will be carried out based on the principles in the Declaration of Helsinki and the International Conference on Harmonisation Good Clinical Practice. Ethics approval has been granted from the Scotland A Research Ethics Committee (REC) and Oxford C REC. On conclusion of this study, the results will be

  7. Correlation of ISS Electric Potential Variations with Mission Operations

    Science.gov (United States)

    Willis, Emily M.; Minow, Joseph I.; Parker, Linda Neergaard

    2014-01-01

    Spacecraft charging on the International Space Station (ISS) is caused by a complex combination of the low Earth orbit plasma environment, space weather events, operations of the high voltage solar arrays, and changes in the ISS configuration and orbit parameters. Measurements of the ionospheric electron density and temperature along the ISS orbit and variations in the ISS electric potential are obtained from the Floating Potential Measurement Unit (FPMU) suite of four plasma instruments (two Langmuir probes, a Floating Potential Probe, and a Plasma Impedance Probe) on the ISS. These instruments provide a unique capability for monitoring the response of the ISS electric potential to variations in the space environment, changes in vehicle configuration, and operational solar array power manipulation. In particular, rapid variations in ISS potential during solar array operations on time scales of tens of milliseconds can be monitored due to the 128 Hz sample rate of the Floating Potential Probe providing an interesting insight into high voltage solar array interaction with the space plasma environment. Comparing the FPMU data with the ISS operations timeline and solar array data provides a means for correlating some of the more complex and interesting ISS electric potential variations with mission operations. In addition, recent extensions and improvements to the ISS data downlink capabilities have allowed more operating time for the FPMU than ever before. The FPMU was operated for over 200 days in 2013 resulting in the largest data set ever recorded in a single year for the ISS. In this paper we provide examples of a number of the more interesting ISS charging events observed during the 2013 operations including examples of rapid charging events due to solar array power operations, auroral charging events, and other charging behavior related to ISS mission operations.

  8. Magnetic field-aligned electric potentials in nonideal plasma flows

    Science.gov (United States)

    Schindler, K.; Hesse, M.; Birn, J.

    1991-01-01

    The electric field component parallel to the magnetic field arising from plasma flows which violate the frozen-in field condition of ideal magnetohydrodynamics is discussed. The quantity of interest is the potential U = integral E parallel ds where the integral is extended along field lines. It is shown that U can be directly related to magnetic field properties, expressed by Euler potentials, even when time-dependence is included. These results are applicable to earth's magnetosphere, to solar flares, to aligned-rotator models of compact objects, and to galactic rotation. On the basis of order-of-magnitude estimates, these results support the view that parallel electric fields associated with nonideal plasma flows might play an important role in cosmic particle acceleration.

  9. Electric Potential and Electric Field Imaging with Dynamic Applications: 2017 Research Award Innovation

    Science.gov (United States)

    Generazio, Ed

    2017-01-01

    The technology and methods for remote quantitative imaging of electrostatic potentials and electrostatic fields in and around objects and in free space is presented. Electric field imaging (EFI) technology may be applied to characterize intrinsic or existing electric potentials and electric fields, or an externally generated electrostatic field may be used for illuminating volumes to be inspected with EFI. The baseline sensor technology (e-Sensor) and its construction, optional electric field generation (quasi-static generator), and current e- Sensor enhancements (ephemeral e-Sensor) are discussed. Critical design elements of current linear and real-time two-dimensional (2D) measurement systems are highlighted, and the development of a three dimensional (3D) EFI system is presented. Demonstrations for structural, electronic, human, and memory applications are shown. Recent work demonstrates that phonons may be used to create and annihilate electric dipoles within structures. Phonon induced dipoles are ephemeral and their polarization, strength, and location may be quantitatively characterized by EFI providing a new subsurface Phonon-EFI imaging technology. Initial results from real-time imaging of combustion and ion flow, and their measurement complications, will be discussed. These new EFI capabilities are demonstrated to characterize electric charge distribution creating a new field of study embracing areas of interest including electrostatic discharge (ESD) mitigation, crime scene forensics, design and materials selection for advanced sensors, combustion science, on-orbit space potential, container inspection, remote characterization of electronic circuits and level of activation, dielectric morphology of structures, tether integrity, organic molecular memory, atmospheric science, and medical diagnostic and treatment efficacy applications such as cardiac polarization wave propagation and electromyography imaging.

  10. Potential of mean force for electrical conductivity of dense plasmas

    Science.gov (United States)

    Starrett, C. E.

    2017-12-01

    The electrical conductivity in dense plasmas can be calculated with the relaxation-time approximation provided that the interaction potential between the scattering electron and the ion is known. To date there has been considerable uncertainty as to the best way to define this interaction potential so that it correctly includes the effects of ionic structure, screening by electrons and partial ionization. Current approximations lead to significantly different results with varying levels of agreement when compared to bench-mark calculations and experiments. We present a new way to define this potential, drawing on ideas from classical fluid theory to define a potential of mean force. This new potential results in significantly improved agreement with experiments and bench-mark calculations, and includes all the aforementioned physics self-consistently.

  11. Use of plant woody species electrical potential for irrigation scheduling.

    Science.gov (United States)

    Ríos-Rojas, Liliana; Morales-Moraga, David; Alcalde, José A; Gurovich, Luis A

    2015-01-01

    The electrical response of plants to environmental stimuli can be measured and quantitatively related to the intensity of several stimulating sources, like temperature, solar radiation, soil water content, evapotranspiration rates, sap flow and dendrometric cycles. These relations can be used to assess the influence of different environmental situations on soil water availability to plants, defined as a steady state condition between leaf transpirative flow and soil water flow to plant roots. A restricted soil water flow due to soil dryness can trigger water stress in plants, if the atmospheric evaporative demand is high, causing partial stomata closure as a physiological response to avoid plant dehydration; water stressed and unstressed plants manifest a differential electrical response. Real time plant electrical response measurements can anticipate actions that prevent the plant reaching actual stress conditions, optimizing stomata gas exchange and photosynthetic rates. An electrophysiological sensor developed in this work, allows remote real-time recording information on plant electrical potential (EP) in the field, which is highly related to EP measurements obtained with a laboratory Keithley voltmeter sensor used in an highly controlled experimental setup. Our electrophysiological sensor is a wireless, autonomous devise, which transmits EP information via Internet to a data server. Using both types of sensors (EP electrodes with a Keithley voltmeter and the electrophysiological sensor), we measured in real time the electrical responses of Persea americana and Prunus domestica plants, to induced water deficits. The differential response for 2 scenarios: irrigation and water restriction is identified by a progressive change in slope on the daily maximal and minimal electric signal values in stressed plants, and a zero-slope for similar signals for well-watered plants. Results show a correspondence between measured signals obtained by our electrophysiological

  12. The The geothermal potentials for electric development in Maluku Province

    Directory of Open Access Journals (Sweden)

    Vijaya Isnaniawardhani

    2018-03-01

    Full Text Available The characteristic of small to medium size islands is the limited amount of natural resources for electric generation. Presently the needs of energy in Maluku Province are supplied by the diesel generation units. The electricity distributes through an isolated grid system of each island. There are 10 separate systems in Maluku Province, namely Ambon, Namlea, Tual, Saumlaki, Mako, Piru, Bula, Masohi, Dobo and Langgur. From the geothermal point of view, this condition is suitable because the nature of the generation is small to medium and the locations are dispersed. The geological condition of Maluku Province is conducive for the formation of geothermal resources. The advanced utilization of geothermal energy in Maluku Province is in Tulehu located about 8 kilometers NE of Ambon. It is expected that 60 MW electric will be produced at the first stage in 2019. A total of 100 MW resources were estimated. Other places of geothermal potentials are Lauke and Tawen both located in Ambon Island with the potentials of 25 MW respectively. In Oma Haruku, Saparua and Nusa Laut the geothermal potentials were estimated to be 25 MW each. The total amount of geothermal energy in Maluku Province is thus, 225 MW which will contribute significantly to the needs of projected 184 MW in the year 2025. Keywords: Maluku Province, geothermal energy, suitable, dispersed, conducive

  13. Research on potential user identification model for electric energy substitution

    Science.gov (United States)

    Xia, Huaijian; Chen, Meiling; Lin, Haiying; Yang, Shuo; Miao, Bo; Zhu, Xinzhi

    2018-01-01

    The implementation of energy substitution plays an important role in promoting the development of energy conservation and emission reduction in china. Energy service management platform of alternative energy users based on the data in the enterprise production value, product output, coal and other energy consumption as a potential evaluation index, using principal component analysis model to simplify the formation of characteristic index, comprehensive index contains the original variables, and using fuzzy clustering model for the same industry user’s flexible classification. The comprehensive index number and user clustering classification based on constructed particle optimization neural network classification model based on the user, user can replace electric potential prediction. The results of an example show that the model can effectively predict the potential of users’ energy potential.

  14. Electrical/optical dual-function redox potential transistor

    Science.gov (United States)

    Li, Shunpu; Wang, Wensi; Xu, Ju; Chu, Daping; Shen, Z. John; Roy, Saibal

    2013-01-01

    We demonstrate a new type of transistors, the electrical/optical “dual-function redox-potential transistors”, which is solution processable and environmentally stable. This device consists of vertically staked electrodes that act as gate, emitter and collector. It can perform as a normal transistor, whilst one electrode which is sensitised by dye enables to generate photocurrent when illuminated. Solution processable oxide-nanoparticles were used to form various functional layers, which allow an electrolyte to penetrate through and, consequently, the current between emitter and collector can be controlled by the gate potential modulated distribution of ions. The result here shows that the device performs with high ON-current under low driving voltage (transistor performance can readily be controlled by photo-illumination. Such device with combined optical and electrical functionalities allows single device to perform the tasks that are usually done by a circuit/system with multiple optical and electrical components, and it is promising for various applications. PMID:24310311

  15. Nanotomography of Cell Surfaces with Evanescent Fields

    Directory of Open Access Journals (Sweden)

    Michael Wagner

    2008-01-01

    Full Text Available The technique of variable-angle total internal reflection fluorescence microscopy (TIRFM and its application to nanotomography of cell surfaces are described. Present applications include (1 3D imaging of chromosomes in their metaphase to demonstrate axial resolution in the nanometre range, (2 measurements of cell-substrate topology, which upon cholesterol depletion shows some loosening of cell-substrate contacts, and (3 measurements of cell topology upon photodynamic therapy (PDT, which demonstrate cell swelling and maintenance of focal contacts. The potential of the method for in vitro diagnostics, but also some requirements and limitations are discussed.

  16. Potential for generation of public electricity in cane sugar factories

    Energy Technology Data Exchange (ETDEWEB)

    Torisson, T.

    1984-04-01

    Sugar cane is the most efficient crop for the conversion of solar energy into biomass. The possibility of conservation of energy in cane sugar producing countries by substituting bagasse for imported oil, was studied in Guyana, South America and financed by the World Bank. The concept of cogeneration was considered, where the heat energy generated by burning bagasse of high fiber content is converted into steam and used both for electricity generation and generation of internal power. Several methods of achieving energy efficiency in this process were discussed such as efficient generation and use of the steam by using high pressure boilers, drying and pelletization of bagasse, and using sugar cane trash as fuel. About 40% of the bagasse could be available for the generation of electric energy. A method for evaluation of the power potential showed that the quantity of public electricity produced, depended on certain important process parameters, fiber content, steam conditions and process steam. The cost effectiveness of the project increases with increasing fiber content in the sugar cane.

  17. Potential for deserts to supply reliable renewable electric power

    Science.gov (United States)

    Labordena, Mercè; Lilliestam, Johan

    2015-04-01

    To avoid dangerous climate change, the electricity systems must be decarbonized by mid-century. The world has sufficient renewable electricity resources for complete power sector decarbonization, but an expansion of renewables poses several challenges for the electricity systems. First, wind and solar PV power are intermittent and supply-controlled, making it difficult to securely integrate this fluctuating generation into the power systems. Consequently, power sources that are both renewable and dispatchable, such as biomass, hydro and concentrating solar power (CSP), are particularly important. Second, renewable power has a low power density and needs vast areas of land, which is problematic both due to cost reasons and due to land-use conflicts, in particular with agriculture. Renewable and dispatchable technologies that can be built in sparsely inhabited regions or on land with low competition with agriculture would therefore be especially valuable; this land-use competition greatly limits the potential for hydro and biomass electricity. Deserts, however, are precisely such low-competition land, and are at the same time the most suited places for CSP generation, but this option would necessitate long transmission lines from remote places in the deserts to the demand centers such as big cities. We therefore study the potential for fleets of CSP plants in the large deserts of the world to produce reliable and reasonable-cost renewable electricity for regions with high and/or rapidly increasing electricity demand and with a desert within or close to its borders. The regions in focus here are the European Union, North Africa and the Middle East, China and Australia. We conduct the analysis in three steps. First, we identify the best solar generation areas in the selected deserts using geographic information systems (GIS), and applying restrictions to minimize impact on biodiversity, soils, human heath, and land-use and land-cover change. Second, we identify

  18. Formation of v-shaped potentials. [auroral zone electric fields

    Science.gov (United States)

    Thiemann, H.; Singh, N.; Schunk, R. W.

    1983-01-01

    The V-shaped potential structures formed by the injection of a non-neutral electron current into a cold background plasma were simulated numerically. The injection disturbs the initial quasi-neutral plasma, leading to the excitation of strong turbulences which heat the plasma. This leads to expulsion of the plasma from the simulation region. Due to ambipolar electric fields the current injection is interrupted and the initial background plasma is extracted from the system. A particle composition with the characteristics of the two plasma reservoirs now represents the plasma in the simulation region. The interaction of the electron beam with this plasma excites turbulences of smaller amplitudes. A nearly constant time averaged potential drop with nonstationary distribution develops across the system. Single and multiple double layers may form for the duration of one ion plasma period.

  19. Solar salt pond potential site survey for electrical power generation

    Science.gov (United States)

    Hurick, M. G.

    1982-01-01

    A solar salt gradient pond acts as a passive heat sink or thermal battery in which energy can be recovered through the conversion of thermal energy into electrical energy. Here, a condensation of a larger report that focused on the identification of potential salt gradient pond sites in the United States using in-situ resources is presented. It is shown that there are at least 24 states that lie in a primary or secondary potential site category. Fourteen states are assigned as primary states and ten are assigned as secondary. The division is subjectively based on the severity of winter weather. The most promising states are those that lie in the southern half of the country. When the primary and secondary category states are combined with the other states that may be able to support a pond, a total of 38 states exhibit the possibility of supporting power generation sites of various size.

  20. Potential impacts of electric vehicles on air quality in Taiwan.

    Science.gov (United States)

    Li, Nan; Chen, Jen-Ping; Tsai, I-Chun; He, Qingyang; Chi, Szu-Yu; Lin, Yi-Chiu; Fu, Tzung-May

    2016-10-01

    The prospective impacts of electric vehicle (EV) penetration on the air quality in Taiwan were evaluated using an air quality model with the assumption of an ambitious replacement of current light-duty vehicles under different power generation scenarios. With full EV penetration (i.e., the replacement of all light-duty vehicles), CO, VOCs, NOx and PM2.5 emissions in Taiwan from a fleet of 20.6 million vehicles would be reduced by 1500, 165, 33.9 and 7.2Ggyr(-1), respectively, while electric sector NOx and SO2 emissions would be increased by up to 20.3 and 12.9Ggyr(-1), respectively, if the electricity to power EVs were provided by thermal power plants. The net impacts of these emission changes would be to reduce the annual mean surface concentrations of CO, VOCs, NOx and PM2.5 by about 260, 11.3, 3.3ppb and 2.1μgm(-3), respectively, but to increase SO2 by 0.1ppb. Larger reductions tend to occur at time and place of higher ambient concentrations and during high pollution events. Greater benefits would clearly be attained if clean energy sources were fully encouraged. EV penetration would also reduce the mean peak-time surface O3 concentrations by up to 7ppb across Taiwan with the exception of the center of metropolitan Taipei where the concentration increased by <2ppb. Furthermore, full EV penetration would reduce annual days of O3 pollution episodes by ~40% and PM2.5 pollution episodes by 6-10%. Our findings offer important insights into the air quality impacts of EV and can provide useful information for potential mitigation actions. Copyright © 2016 Elsevier B.V. All rights reserved.

  1. The Mycobacterium tuberculosis cell-surface glycoprotein apa as a potential adhesin to colonize target cells via the innate immune system pulmonary C-type lectin surfactant protein A.

    Science.gov (United States)

    Ragas, Aude; Roussel, Lucie; Puzo, Germain; Rivière, Michel

    2007-02-23

    Tuberculosis is still a major health problem, and understanding the mechanism by which Mycobacterium tuberculosis (Mtb) invades and colonizes its host target cells remains an important issue for the control of infection. The innate immune system C-type lectins (C-TLs), including the human pulmonary surfactant protein A (PSP-A), have been recently identified as determinant players in the early recognition of the invading pathogen and in mounting the host defense response. Although the antigenic lipoglycan mannosylated lipoarabinomannan is currently considered to be the major C-TL target on the mycobacterial surface, the recognition by some C-TLs of the only mycobacterial species composing the "Mtb complex" indicates that mannosylated lipoarabinomannan cannot account alone for this specificity. Thus, we searched for the mycobacterial molecules targeted by human PSP-A, focusing our attention on the Mtb surface glycoproteins. We developed an original functional proteomic approach based on a lectin blot assay using crude human bronchoalveolar lavage fluid as a source of physiological PSP-A. Combined with selective cell-surface protein extraction and mass spectrometry peptide mapping, this strategy allowed us to identify the Apa (alanine- and proline-rich antigenic) glycoprotein as new potential target for PSP-A. This result was supported by direct binding of PSP-A to purified Apa. Moreover, EDTA addition or deglycosylation of purified Apa samples completely abolished the interaction, demonstrating that the interaction is calcium- and mannose-dependent, as expected. Finally, we provide convincing evidence that Apa, formerly considered as mainly secreted, is associated with the cell wall for a sufficiently long time to aid in the attachment of PSP-A. Because, to date, Apa seems to be restricted to the Mtb complex strains, we propose that it may account for the selective recognition of those strains by PSP-A and other immune system C-TLs containing homologous functional

  2. Effect of electrical potential of microbubbles on ozone dissolution

    Science.gov (United States)

    Hasegawa, H.; Kataoka, H.; Asano, K.

    2009-02-01

    Microbubbles make ozone water generation effective due to the high dissolution rate of gas in contrast to a conventional generating method. Therefore, it is presumable that ozone water generation using microbubbles can be achieved by the low concentration ozone gas. In our previous study, a compact and low power microbubble generator was developed. The microbubbles are generated by the local shear stress in the flow through a pipe with slits. In the present study, in order to investigate the relationship between the electrical potential of the gas-water interface and the cleaning of cloth using ozone microbubbles, two models with different slit angles (θ=30 and 60 deg) were installed. High concentration ozone water is produced for θ = 60 deg in contrast to the θ=30 deg case. When a cloth is washed in the θ=60 deg case, the soiled cloth can be cleaned easily in comparison with the θ=30 deg case, because the zeta potential of microbubbles for θ=60 deg is larger than that for θ=30 deg.

  3. Load shift potential of electric vehicles in Europe

    Science.gov (United States)

    Babrowski, Sonja; Heinrichs, Heidi; Jochem, Patrick; Fichtner, Wolf

    2014-06-01

    Many governments highly encourage electric mobility today, aiming at a high market penetration. This development would bring forth an impact on the energy system, which strongly depends on the driving and charging behavior of the users. While an uncontrolled immediate charging might strain the local grid and/or higher peak loads, there are benefits to be gained by a controlled charging. We examine six European mobility studies in order to display the effects of controlled and uncontrolled unidirectional charging. Taking into account country-specific driving patterns, we generate for each country a charging load curve corresponding to uncontrolled charging and consider the corresponding parking time at charging facilities in order to identify load shift potentials. The main results are that besides the charging power of the vehicles, the possibility to charge at the work place has a significant influence on the uncontrolled charging curve. Neither national nor regional differences are as significant. When charging is only possible at home, the vehicle availability at charging facilities during the day for all countries is at least 24%. With the additional possibility to charge at work, at least 45% are constantly available. Accordingly, we identified a big potential for load shifting through controlled charging.

  4. Ionospheric potential variability in global electric circuit models (Invited)

    Science.gov (United States)

    Mareev, E.; Volodin, E. M.; Kalinin, A.; Sllyunyaev, N.

    2013-12-01

    The ionospheric potential (IP) represents the electric voltage between the Earth's surface and the lower ionosphere and may be measured with a sufficient accuracy using the balloon soundings over the lowest 15-20 km. This parameter can serve as a global index relating the state of the global electric circuit (GEC) to the planetary climate. Exploring the GEC as a diagnostic tool for climate studies requires an accurate modeling of the IP stationary state and its dynamics, while a question of secular trend of the IP is still under discussion (Markson, 2007; Williams, 2009; Williams and Mareev, 2013). This paper addresses a possibility of correct calculation of the IP in 3D models of the GEC and its adequate parameterization to be used in General Circulation Models (GCM). Our approach is based on the use the integral representation for the contribution of charging currents, supporting the generators (in particular, electrified clouds) in the GEC, into the ionospheric potential (Kalinin et al., 2011; Mareeva et al., 2011). Simple enough analytical expressions for IP induced by the charging electric currents are suggested, including the contribution of the Austausch generator. We have developed also the spherical numerical model of the GEC and applied it for IP calculation for different-type cloud contribution into the circuit. A suggested IP parameterization is appropriate for the use in climate-model simulations (Mareev and Volodin, 2011). We use a high-resolution GCM of the atmosphere and ocean INMCM4.0 for the modeling the GEC. The main characteristics of the model are: atmosphere - 2x1.5 degrees in longitude and latitude, 21 levels; ocean - 1x0.5 degrees in longitude and latitude, 40 levels. We have taken into account quasi-stationary currents of electrified clouds as principal contributors into the DC global circuit. One of the most important aspects of this approach is an account for all the electrified clouds- both thunderstorms and electrified shower cloud. The

  5. Load management potential of electric vehicles; Lastmanagementpotential Elektrofahrzeuge

    Energy Technology Data Exchange (ETDEWEB)

    Agsten, Michael; Schlegel, Steffen; Westermann, Dirk [Technische Univ. Ilmenau (Germany)

    2011-07-01

    The introduction of battery electric vehicles, allows to minimize the participation of fossile energy by controlling the charging process with respect to renewables, like wind, solar or hydro power. First investigations have shown a concentration of charging power during already existing peak power periods. As a matter of fact there will be an overload on distribution grid devices. The following paper deals with the modeling and optimization of the expected electric load caused by electric vehicles. (orig.)

  6. Carbon Nanotubes and Graphene Nanoribbons: Potentials for Nanoscale Electrical Interconnects

    Directory of Open Access Journals (Sweden)

    Swastik Kar

    2013-08-01

    Full Text Available Carbon allotropes have generated much interest among different scientific communities due to their peculiar properties and potential applications in a variety of fields. Carbon nanotubes and more recently graphene have shown very interesting electrical properties along with the possibility of being grown and/or deposited at a desired location. In this Review, we will focus our attention on carbon-based nanostructures (in particular, carbon nanotubes and graphene nanoribbons which could play an important role in the technological quest to replace copper/low-k for interconnect applications. We will provide the reader with a number of possible architectures, including single-wall as well as multi-wall carbon nanotubes, arranged in horizontal and vertical arrays, regarded as individual objects as well as bundles. Modification of their functional properties in order to fulfill interconnect applications requirements are also presented. Then, in the second part of the Review, recently discovered graphene and in particular graphene and few-graphene layers nanoribbons are introduced. Different architectures involving nanostructured carbon are presented and discussed in light of interconnect application in terms of length, chirality, edge configuration and more.

  7. Protective role of allicin (diallyl thiosulfinate) on cell surface ...

    African Journals Online (AJOL)

    altered glycoconjugates in plasma, buccal mucosa tumour tissues and erythrocyte membrane of tumour bearing hamsters were normalized after treated with allicin. Conclusion: The results suggest that allicin has considerable potential to protect and restore the cell surface glycoconjugates moieties in the presence of allicin ...

  8. Protective role of allicin (diallyl thiosulfinate) on cell surface ...

    African Journals Online (AJOL)

    DMBAaltered glycoconjugates in plasma, buccal mucosa tumour tissues and erythrocyte membrane of tumour bearing hamsters were normalized after treated with allicin. Conclusion: The results suggest that allicin has considerable potential to protect and restore the cell surface glycoconjugates moieties in the presence of ...

  9. Potentiality Prediction of Electric Power Replacement Based on Power Market Development Strategy

    Science.gov (United States)

    Miao, Bo; Yang, Shuo; Liu, Qiang; Lin, Jingyi; Zhao, Le; Liu, Chang; Li, Bin

    2017-05-01

    The application of electric power replacement plays an important role in promoting the development of energy conservation and emission reduction in our country. To exploit the potentiality of regional electric power replacement, the regional GDP (gross domestic product) and energy consumption are taken as potentiality evaluation indicators. The principal component factors are extracted with PCA (principal component analysis), and the integral potentiality analysis is made to the potentiality of electric power replacement in the national various regions; a region is taken as a research object, and the potentiality of electric power replacement is defined and quantified. The analytical model for the potentiality of multi-scenario electric power replacement is developed, and prediction is made to the energy consumption with the grey prediction model. The relevant theoretical research is utilized to realize prediction analysis on the potentiality amount of multi-scenario electric power replacement.

  10. Facilitation and refractoriness of the electrically evoked compound action potential.

    Science.gov (United States)

    Hey, Matthias; Müller-Deile, Joachim; Hessel, Horst; Killian, Matthijs

    2017-11-01

    In this study we aim to resolve the contributions of facilitation and refractoriness at very short pulse intervals. Measurements of the refractory properties of the electrically evoked compound action potential (ECAP) of the auditory nerve in cochlear implant (CI) users at inter pulse intervals below 300 μs are influenced by facilitation and recovery effects. ECAPs were recorded using masker pulses with a wide range of current levels relative to the probe pulse levels, for three suprathreshold probe levels and pulse intervals from 13 to 200 μs. Evoked potentials were measured for 21 CI patients by using the masked response extraction artifact cancellation procedure. During analysis of the measurements the stimulation current was not used as absolute value, but in relation to the patient's individual ECAP threshold. This enabled a more general approach to describe facilitation as a probe level independent effect. Maximum facilitation was found for all tested inter pulse intervals at masker levels near patient's individual ECAP threshold, independent from probe level. For short inter pulse intervals an increased N1P1 amplitude was measured for subthreshold masker levels down to 120 CL below patient's individual ECAP threshold in contrast to the recreated state. ECAPs recorded with inter pulse intervals up to 200 μs are influenced by facilitation and recovery. Facilitation effects are most pronounced for masker levels at or below ECAP threshold, while recovery effects increase with higher masker levels above ECAP threshold. The local maximum of the ECAP amplitude for masker levels around ECAP threshold can be explained by the mutual influence of maximum facilitation and minimal refractoriness. Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.

  11. A Marked Point Process Framework for Extracellular Electrical Potentials

    Directory of Open Access Journals (Sweden)

    Carlos A. Loza

    2017-12-01

    Full Text Available Neuromodulations are an important component of extracellular electrical potentials (EEP, such as the Electroencephalogram (EEG, Electrocorticogram (ECoG and Local Field Potentials (LFP. This spatially temporal organized multi-frequency transient (phasic activity reflects the multiscale spatiotemporal synchronization of neuronal populations in response to external stimuli or internal physiological processes. We propose a novel generative statistical model of a single EEP channel, where the collected signal is regarded as the noisy addition of reoccurring, multi-frequency phasic events over time. One of the main advantages of the proposed framework is the exceptional temporal resolution in the time location of the EEP phasic events, e.g., up to the sampling period utilized in the data collection. Therefore, this allows for the first time a description of neuromodulation in EEPs as a Marked Point Process (MPP, represented by their amplitude, center frequency, duration, and time of occurrence. The generative model for the multi-frequency phasic events exploits sparseness and involves a shift-invariant implementation of the clustering technique known as k-means. The cost function incorporates a robust estimation component based on correntropy to mitigate the outliers caused by the inherent noise in the EEP. Lastly, the background EEP activity is explicitly modeled as the non-sparse component of the collected signal to further improve the delineation of the multi-frequency phasic events in time. The framework is validated using two publicly available datasets: the DREAMS sleep spindles database and one of the Brain-Computer Interface (BCI competition datasets. The results achieve benchmark performance and provide novel quantitative descriptions based on power, event rates and timing in order to assess behavioral correlates beyond the classical power spectrum-based analysis. This opens the possibility for a unifying point process framework of

  12. Accuracy of measurement in electrically evoked compound action potentials.

    Science.gov (United States)

    Hey, Matthias; Müller-Deile, Joachim

    2015-01-15

    Electrically evoked compound action potentials (ECAP) in cochlear implant (CI) patients are characterized by the amplitude of the N1P1 complex. The measurement of evoked potentials yields a combination of the measured signal with various noise components but for ECAP procedures performed in the clinical routine, only the averaged curve is accessible. To date no detailed analysis of error dimension has been published. The aim of this study was to determine the error of the N1P1 amplitude and to determine the factors that impact the outcome. Measurements were performed on 32 CI patients with either CI24RE (CA) or CI512 implants using the Software Custom Sound EP (Cochlear). N1P1 error approximation of non-averaged raw data consisting of recorded single-sweeps was compared to methods of error approximation based on mean curves. The error approximation of the N1P1 amplitude using averaged data showed comparable results to single-point error estimation. The error of the N1P1 amplitude depends on the number of averaging steps and amplification; in contrast, the error of the N1P1 amplitude is not dependent on the stimulus intensity. Single-point error showed smaller N1P1 error and better coincidence with 1/√(N) function (N is the number of measured sweeps) compared to the known maximum-minimum criterion. Evaluation of N1P1 amplitude should be accompanied by indication of its error. The retrospective approximation of this measurement error from the averaged data available in clinically used software is possible and best done utilizing the D-trace in forward masking artefact reduction mode (no stimulation applied and recording contains only the switch-on-artefact). Copyright © 2014 Elsevier B.V. All rights reserved.

  13. Vertical electrical sounding to delineate the potential aquifer zones ...

    Indian Academy of Sciences (India)

    Joy Choudhury

    2017-09-06

    Sep 6, 2017 ... direct current (DC) resistivity soundings method also known as vertical electrical sounding (VES) is one of the most applied ... Vertical electrical sounding; groundwater; aquifer zones; borehole litholog; Niamey. 1. Introduction. Niger is a ..... Telford M, Geldart L P, Sheriff R E and Keys D A 1976. Applied ...

  14. A preliminary assessment of the potential risks from electrical ...

    African Journals Online (AJOL)

    concern in Kenya, 17 (71 %) face a high risk of direct interactions with electrical infrastructure. Priority species ... A rapid preliminary risk assessment of electrical infrastructure was conducted in two areas of Kenya: i) ..... The fundamentals and practice of Overhead Line Maintenance (132kV and above), pp217-245. Eskom ...

  15. Survey of wind power potential for wind-based electricity

    African Journals Online (AJOL)

    Mgina

    2006-12-31

    Dec 31, 2006 ... for 12 months. INTRODUCTION. Generation of electricity world wide is mainly from fossil fuels, renewable and nuclear sources. Fossil fuel sources currently provide a larger share of world electricity generation than any other energy resource and are expected to remain in that position. f o r. m o r e. t h a n a.

  16. Inhibition potentiates the synchronizing action of electrical synapses

    Directory of Open Access Journals (Sweden)

    Benjamin Pfeuty

    2007-11-01

    Full Text Available In vivo and in vitro experimental studies have found that blocking electrical interactions connecting GABAergic interneurons reduces oscillatory activity in the µ range in cortex. However, recent theoretical works have shown that the ability of electrical synapses to promote or impede synchrony, when alone, depends on their location on the dendritic tree of the neurons, the intrinsic properties of the neurons and the connectivity of the network. The goal of the present paper is to show that this versatility in the synchronizing ability of electrical synapses is greatly reduced when the neurons also interact via inhibition. To this end, we study a model network comprising two-compartment conductance-based neurons interacting with both types of synapses. We investigate the effect of electrical synapses on the dynamical state of the network as a function of the strength of the inhibition. We find that for weak inhibition, electrical synapses reinforce inhibition-generated synchrony only if they promote synchrony when they are alone. In contrast, when inhibition is sufficiently strong, electrical synapses improve synchrony even if when acting alone they would stabilize asynchronous firing. We clarify the mechanism underlying this cooperative interplay between electrical and inhibitory synapses. We show that it is relevant in two physiologically observed regimes: spike-to-spike synchrony, where neurons fire at almost every cycle of the population oscillations, and stochastic synchrony, where neurons fire irregularly and at a rate which is substantially lower than the frequency of the global population rhythm.

  17. Multipurpose Electric Potential Sensor for Spacecraft Applications Project

    Data.gov (United States)

    National Aeronautics and Space Administration — The original goal of Phase I was to study the feasibility of developing an electric sensor that can be used for as many NASA sensing applications as possible. During...

  18. Characterizing soil erosion potential using electrical resistivity imaging : final report.

    Science.gov (United States)

    2017-04-01

    The erosion rate, or erodibility, of soil depends on many soil characteristics including: plasticity, : water content, grain size, percent clay, compaction, and shear strength. Many of these characteristics also : influence soil in situ bulk electric...

  19. Characterizing soil erosion potential using electrical resistivity imaging : technical summary.

    Science.gov (United States)

    2017-04-01

    The erosion rate, or erodibility, of soil depends on many soil characteristics : including: plasticity, water content, grain size, percent clay, compaction, and shear : strength. Many of these characteristics also influence soil in situ bulk electric...

  20. Electric potential invariants and ions-in-molecules effective potentials for molecular Rydberg states

    Science.gov (United States)

    Coy, Stephen L.; Grimes, David D.; Zhou, Yan; Field, Robert W.; Wong, Bryan M.

    2016-12-01

    The dependence of multipole moments and polarizabilities on external fields appears in many applications including biomolecular molecular mechanics, optical non-linearity, nanomaterial calculations, and the perturbation of spectroscopic signatures in atomic clocks. Over a wide range of distances, distributed multipole and polarizability potentials can be applied to obtain the variation of atom-centered atoms-in-molecules electric properties like bonding-quenched polarizability. For cylindrically symmetric charge distributions, we examine single-center and atom-centered effective polarization potentials in a non-relativistic approximation for Rydberg states. For ions, the multipole expansion is strongly origin-dependent, but we note that origin-independent invariants can be defined. The several families of invariants correspond to optimized representations differing by origin and number of terms. Among them, a representation at the center of dipole polarizability optimizes the accuracy of the potential with terms through 1/r4. We formulate the single-center expansion in terms of polarization-modified effective multipole moments, defining a form related to the source-multipole expansion of Brink and Satchler. Atom-centered potentials are an origin independent alternative but are limited both by the properties allowed at each center and by the neglected effects like bond polarizability and charge flow. To enable comparisons between single-center effective potentials in Cartesian or spherical form and two-center effective potentials with differing levels of mutual induction between atomic centers, we give analytical expressions for the bond-length and origin-dependence of multipole and polarizability terms projected in the multipole and polarizability expansion of Buckingham. The atom-centered potentials can then be used with experimental data and ab initio calculations to estimate atoms-in-molecules properties. Some results are given for BaF+ and HF showing the

  1. Microbial cell surfaces and secretion systems

    NARCIS (Netherlands)

    Tommassen, J.P.M.|info:eu-repo/dai/nl/069127077; Wosten, H.A.B.|info:eu-repo/dai/nl/120693186

    2015-01-01

    Microbial cell surfaces, surface-exposed organelles, and secreted proteins are important for the interaction with the environment, including adhesion to hosts, protection against host defense mechanisms, nutrient acquisition, and intermicrobial competition. Here, we describe the structures of the

  2. Analysis on Potential of Electric Energy Market based on Large Industrial Consumer

    Science.gov (United States)

    Lin, Jingyi; Zhu, Xinzhi; Yang, Shuo; Xia, Huaijian; Yang, Di; Li, Hao; Lin, Haiying

    2018-01-01

    The implementation of electric energy substitution by enterprises plays an important role in promoting the development of energy conservation and emission reduction in china. In order to explore alternative energy potential of industrial enterprises, to simulate and analyze the process of industrial enterprises, identify high energy consumption process and equipment, give priority to alternative energy technologies, and determine the enterprise electric energy substitution potential predictive value, this paper constructs the evaluation model of the influence factors of the electric energy substitution potential of industrial enterprises, and uses the combined weight method to determine the weight value of the evaluation factors to calculate the target value of the electric energy substitution potential. Taking the iron and steel industry as an example, this method is used to excavate the potential. The results show that the method can effectively tap the potential of the electric power industry

  3. Microbial cell surfaces and secretion systems

    OpenAIRE

    Tommassen, J.P.M.; Wosten, H.A.B.

    2015-01-01

    Microbial cell surfaces, surface-exposed organelles, and secreted proteins are important for the interaction with the environment, including adhesion to hosts, protection against host defense mechanisms, nutrient acquisition, and intermicrobial competition. Here, we describe the structures of the cell envelopes of bacteria , fungi, and oomycetes , and the mechanisms they have evolved for the transport of proteins across these envelopes to the cell surface and into the extracellular milieu.

  4. Quantifying the Relationship Between Curvature and Electric Potential in Lipid Bilayers

    DEFF Research Database (Denmark)

    Bruhn, Dennis Skjøth; Lomholt, Michael Andersen; Khandelia, Himanshu

    2016-01-01

    Cellular membranes mediate vital cellular processes by being subject to curvature and transmembrane electrical potentials. Here we build upon the existing theory for flexoelectricity in liquid crystals to quantify the coupling between lipid bilayer curvature and membrane potentials. Using molecul...

  5. Cell Surface Proteome of Dental Pulp Stem Cells Identified by Label-Free Mass Spectrometry.

    Directory of Open Access Journals (Sweden)

    Christian Niehage

    Full Text Available Multipotent mesenchymal stromal cells (MSCs are promising tools for regenerative medicine. They can be isolated from different sources based on their plastic-adherence property. The identification of reliable cell surface markers thus becomes the Holy Grail for their prospective isolation. Here, we determine the cell surface proteomes of human dental pulp-derived MSCs isolated from single donors after culture expansion in low (2% or high (10% serum-containing media. Cell surface proteins were tagged on intact cells using cell impermeable, cleavable sulfo-NHS-SS-biotin, which allows their enrichment by streptavidin pull-down. For the proteomic analyses, we first compared label-free methods to analyze cell surface proteomes i.e. composition, enrichment and proteomic differences, and we developed a new mathematical model to determine cell surface protein enrichment using a combinatorial gene ontology query. Using this workflow, we identified 101 cluster of differentiation (CD markers and 286 non-CD cell surface proteins. Based on this proteome profiling, we identified 14 cell surface proteins, which varied consistently in abundance when cells were cultured under low or high serum conditions. Collectively, our analytical methods provide a basis for identifying the cell surface proteome of dental pulp stem cells isolated from single donors and its evolution during culture or differentiation. Our data provide a comprehensive cell surface proteome for the precise identification of dental pulp-derived MSC populations and their isolation for potential therapeutic intervention.

  6. Potential Analysis of Electric Vehicle (EV) Grid Integration

    DEFF Research Database (Denmark)

    Wu, Qiuwei; Nielsen, Arne Hejde; Østergaard, Jacob

    2011-01-01

    Electric vehicles (EVs) have been considered as distributed energy resources (DER) to handle the fluctuation from renewable energy resources (RES), especially the wind power. The intelligent management of EV charging and discharging can achieve the goal of providing up and down regulating power...

  7. A preliminary assessment of the potential risks from electrical ...

    African Journals Online (AJOL)

    A rapid risk assessment of the interactions between Kenya's large birds and electrical infrastructure was conducted around Magadi and Naivasha in Kenya in January 2009. Six out of the seven <132 kV distribution pole designs assessed pose an electrocution risk to medium and large-sized birds. Several sites of high bird ...

  8. assessment of wind energy potential for electricity generation in ...

    African Journals Online (AJOL)

    Renewable energy sources such as wind, if thoroughly investigated, could be used to reduce the dependence on fossil fuels for electricity generation. Although wind energy is one of the most efficient renewable energy sources, it is very variable compared to other sources of energy. It is also more sensitive to variations with ...

  9. assessment of wind energy potential for electricity generation in ...

    African Journals Online (AJOL)

    Wind energy is proposed as an alternative source of electricity to fossil fuel generators ... it is very variable compared to other sources of energy. It is also more sensitive to variations with topography and weather patterns compared to solar energy. ... A study on wind speed pattern and the available wind power in Tanzania.

  10. In situ electron holography of electric potentials inside a solid-state electrolyte: Effect of electric-field leakage

    Energy Technology Data Exchange (ETDEWEB)

    Aizawa, Yuka; Yamamoto, Kazuo; Sato, Takeshi [Nanostructures Research Laboratory, Japan Fine Ceramics Center, 2-4-1 Mutsuno, Atsuta-ku, Nagoya, Aichi 456-8587 (Japan); Murata, Hidekazu [Faculty of Science and Technology, Meijo University, 1-501 Shiogamaguchi, Tempaku-ku, Nagoya, Aichi 468-8502 (Japan); Yoshida, Ryuji; Fisher, Craig A.J. [Nanostructures Research Laboratory, Japan Fine Ceramics Center, 2-4-1 Mutsuno, Atsuta-ku, Nagoya, Aichi 456-8587 (Japan); Kato, Takehisa; Iriyama, Yasutoshi [Department of Materials, Physics and Energy Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Aichi 464-8601 (Japan); Hirayama, Tsukasa, E-mail: t-hirayama@jfcc.or.jp [Nanostructures Research Laboratory, Japan Fine Ceramics Center, 2-4-1 Mutsuno, Atsuta-ku, Nagoya, Aichi 456-8587 (Japan)

    2017-07-15

    In situ electron holography is used to observe changes of electric-potential distributions in an amorphous lithium phosphorus oxynitride (LiPON) solid-state electrolyte when different voltages are applied. 2D phase images are simulated by integrating the 3D potential distribution along the electron trajectory through a thin Cu/LiPON/Cu region. Good agreement between experimental and simulated phase distributions is obtained when the influence of the external electric field is taken into account using the 3D boundary-charge method. Based on the precise potential changes, the lithium-ion and lithium-vacancy distributions inside the LiPON layer and electric double layers (EDLs) are inferred. The gradients of the phase drops at the interfaces in relation to EDL widths are discussed. - Highlights: • Solid-state electrolyte LiPON has been observed by in situ electron holography. • Observed phase distributions are compared with those simulated numerically. • 3D electric fields around the specimen are taken into account in the simulation. • Electric-potential distributions inside LiPON have been obtained. • The lithium-ion and lithium-vacancy distributions inside the LiPON are inferred.

  11. Photoionization cross section in a spherical quantum dot: Effects of some parabolic confining electric potentials

    Directory of Open Access Journals (Sweden)

    M. Tshipa

    2017-12-01

    Full Text Available A theoretical investigation of the effects of spatial variation of confining electric potential on photoionization cross section (PCS in a spherical quantum dot is presented. The potential profiles considered here are the shifted parabolic potential and the inverse lateral shifted parabolic potential compared with the well-studied parabolic potential. The primary findings are that parabolic potential and the inverse lateral shifted parabolic potential blue shift the peaks of the PCS while the shifted parabolic potential causes a red shift.

  12. Potential of Reversible Solid Oxide Cells as Electricity Storage System

    Directory of Open Access Journals (Sweden)

    Paolo Di Giorgio

    2016-08-01

    Full Text Available Electrical energy storage (EES systems allow shifting the time of electric power generation from that of consumption, and they are expected to play a major role in future electric grids where the share of intermittent renewable energy systems (RES, and especially solar and wind power plants, is planned to increase. No commercially available technology complies with all the required specifications for an efficient and reliable EES system. Reversible solid oxide cells (ReSOC working in both fuel cell and electrolysis modes could be a cost effective and highly efficient EES, but are not yet ready for the market. In fact, using the system in fuel cell mode produces high temperature heat that can be recovered during electrolysis, when a heat source is necessary. Before ReSOCs can be used as EES systems, many problems have to be solved. This paper presents a new ReSOC concept, where the thermal energy produced during fuel cell mode is stored as sensible or latent heat, respectively, in a high density and high specific heat material and in a phase change material (PCM and used during electrolysis operation. The study of two different storage concepts is performed using a lumped parameters ReSOC stack model coupled with a suitable balance of plant. The optimal roundtrip efficiency calculated for both of the configurations studied is not far from 70% and results from a trade-off between the stack roundtrip efficiency and the energy consumed by the auxiliary power systems.

  13. Electric Mars: A Large Trans-Terminator Electric Potential Drop on Closed Magnetic Field Lines Above Utopia Planitia

    Science.gov (United States)

    Collinson, Glyn; Mitchell, David; Xu, Shaosui; Glocer, Alex; Grebowsky, Joseph; Hara, Takuya; Lillis, Robert; Espley, Jared; Mazelle, Christian; Sauvaud, Jean-Andre

    2017-01-01

    Abstract Parallel electric fields and their associated electric potential structures play a crucial role inionospheric-magnetospheric interactions at any planet. Although there is abundant evidence that parallel electric fields play key roles in Martian ionospheric outflow and auroral electron acceleration, the fields themselves are challenging to directly measure due to their relatively weak nature. Using measurements by the Solar Wind Electron Analyzer instrument aboard the NASA Mars Atmosphere and Volatile EvolutioN(MAVEN) Mars Scout, we present the discovery and measurement of a substantial (Phi) Mars 7.7 +/-0.6 V) parallel electric potential drop on closed magnetic field lines spanning the terminator from day to night above the great impact basin of Utopia Planitia, a region largely free of crustal magnetic fields. A survey of the previous 26 orbits passing over a range of longitudes revealed similar signatures on seven orbits, with a mean potential drop (Phi) Mars of 10.9 +/- 0.8 V, suggestive that although trans-terminator electric fields of comparable strength are not ubiquitous, they may be common, at least at these northerly latitudes.

  14. Electric field as a potential directional cue in homing of bone marrow-derived mesenchymal stem cells to cutaneous wounds.

    Science.gov (United States)

    Zimolag, Eliza; Borowczyk-Michalowska, Julia; Kedracka-Krok, Sylwia; Skupien-Rabian, Bozena; Karnas, Elzbieta; Lasota, Slawomir; Sroka, Jolanta; Drukala, Justyna; Madeja, Zbigniew

    2017-02-01

    Bone marrow-derived cells are thought to participate and enhance the healing process contributing to skin cells or releasing regulatory cytokines. Directional cell migration in a weak direct current electric field (DC-EF), known as electrotaxis, may be a way of cell recruitment to the wound site. Here we examined the influence of electric field on bone marrow adherent cells (BMACs) and its potential role as a factor attracting mesenchymal stem cells to cutaneous wounds. We observed that in an external EF, BMAC movement was accelerated and highly directed with distinction of two cell populations migrating toward opposite poles: mesenchymal stem cells migrated toward the cathode, whereas macrophages toward the anode. Analysis of intracellular pathways revealed that macrophage electrotaxis mostly depended on Rho family small GTPases and calcium ions, but interruption of PI3K and Arp2/3 had the most pronounced effect on electrotaxis of MSCs. However, in all cases we observed only a partial decrease in directionality of cell movement after inhibition of certain proteins. Additionally, although we noticed the accumulation of EGFR at the cathodal side of MSCs, it was not involved in electrotaxis. Moreover, the cell reaction to EF was very dynamic with first symptoms occurring within <1min. In conclusion, the physiological DC-EF may act as a factor positioning bone marrow cells within a wound bed and the opposite direction of MSC and macrophage movement did not result either from utilizing different signalling or redistribution of investigated cell surface receptors. Copyright © 2016 The Authors. Published by Elsevier B.V. All rights reserved.

  15. Nanostructuring of Solar Cell Surfaces

    DEFF Research Database (Denmark)

    Davidsen, Rasmus Schmidt; Schmidt, Michael Stenbæk

    Solar energy is by far the most abundant renewable energy source available, but the levelized cost of solar energy is still not competitive with that of fossil fuels. Therefore there is a need to improve the power conversion effciency of solar cells without adding to the production cost. The main...... objective of this PhD thesis is to develop nanostructured silicon (Si) solar cells with higher power conversion efficiency using only scalable and cost-efficient production methods. The nanostructures, known as 'black silicon', are fabricated by single-step, maskless reactive ion etching and used as front....... This result indicates the potential of improved cell performance and higher output power at diffuse light conditions and during daily and yearly operation. A second batch of RIEtextured solar cells with laser-doped selective emitters (LDSE) was fabricated. A power conversion eciency of 18.1% and a ll factor...

  16. Potential of an electric prosthesis for dynamic facial reanimation.

    Science.gov (United States)

    Griffin, Garrett R; Kim, Jennifer C

    2011-09-01

    Chronic facial paralysis is a devastating condition with severe functional and emotional consequences. The current surgical armamentarium permits the predictable reestablishment of a protective blink as well as good resting symmetry. Yet the ultimate goal of symmetric, spontaneous emotional expression remains elusive despite significant progress in the areas of peripheral nerve grafting and free tissue transfer. This commentary explores the possibility of an implantable electrical prosthesis for facial reanimation. It reviews animal studies supporting this concept as well as recent human data suggesting that such an implant could rescue denervated facial musculature, thus overcoming a major hurdle for existing reanimation techniques.

  17. Biosensor regeneration via substrate electric potential: A physical mechanism

    Science.gov (United States)

    Jiang, Rui-Bin; Lei, U.

    2010-06-01

    Literatures showed that the immune type biosensors can be regenerated by applying a voltage (ΔΦ) across the combined macromolecules but the underlying physics was not clarified. By incorporating an electric double layer force and a van der Waals force into a weight-ensemble Brownian dynamics simulation, we found that the dissociation rate constant for biotin-streptavidin increases exponentially with ΔΦ, and reaches 418-fold when ΔΦ equals 1 V. Macroscopic diffusion simulations using such enhanced dissociation rate constants agree with the previous experiments, and explain quantitatively the finding that the regeneration using square-wave voltage is superior to that using saw-tooth voltage.

  18. Functions of proteoglycans at the cell surface

    DEFF Research Database (Denmark)

    Höök, M; Woods, A; Johansson, S

    1986-01-01

    Proteoglycans (primarily heparan sulphate proteoglycans) are found at the surface of most adherent eukaryotic cells. Earlier studies suggest that these molecules can be associated with the cell surface principally by two different mechanisms. Proteoglycans may occur as membrane......-intercalated glycoproteins, where the core protein of the proteoglycan is anchored in the lipid interior of the plasma membrane, or they may be bound via the polysaccharide components of the molecule to specific anchoring proteins present at the cell surface. A number of functions have been proposed for cell surface......-associated proteoglycans, including: regulation of cell-substrate adhesion; regulation of cell proliferation; participation in the binding and uptake of extracellular components; and participation in the regulation of extracellular matrix formation. Evidence is discussed suggesting that the cell-associated heparan...

  19. Electric and Magnetic Coulomb Potentials in the Deuteron

    Directory of Open Access Journals (Sweden)

    Bernard Schaeffer

    2013-09-01

    Full Text Available After one century of nuclear physics, the underlying fun- damental laws of nuclear physics are still missing. Bohr had found a formula for the H atom and another for the H2 molecule but no equivalent formula exists for the deuteron 2H. The only known Coulomb interaction in a nucleus by the mainstream nuclear physics is the long range repulsion between protons, forgetting that the neutron contains elec- tric charges with no net charge. The neutron is attracted by the proton in a way discovered two millenaries ago by the Greeks. This attraction is equilibrated by the repulsion between the opposite magnetic moments of the proton and of the neutron in the deuteron. The bare application of ge- ometry together with electric and magnetic Coulomb’s in- teractions accounts for the binding energy of the deuteron, without fitting, with only 4 per cent discrepancy, proving the electromagnetic nature of the nuclear energy.

  20. Defining Electric Potential Difference by Moving a Multimeter's Ground Probe

    Science.gov (United States)

    Stoeckel, Marta R.

    2018-01-01

    The abstract nature of electric potential difference (voltage) can make it a difficult concept to grasp, but understanding the relative nature of voltage is central to developing a conceptual understanding of electric circuits. In laboratory situations, I see these conceptual difficulties manifest when students have difficulty placing voltmeter…

  1. Effects of electrolysis time and electric potential on chlorine generation of electrolyzed deep ocean water

    Directory of Open Access Journals (Sweden)

    Guoo-Shyng Wang Hsu

    2017-10-01

    Full Text Available Electrolyzed water is a sustainable disinfectant, which can comply with food safety regulations and is environmentally friendly. A two-factor central composite design was adopted for studying the effects of electrolysis time and electric potential on the chlorine generation efficiency of electrolyzed deep ocean water (DOW. DOW was electrolyzed in a glass electrolyzing cell equipped with platinum–plated titanium anode and cathode. The results showed that chlorine concentration reached maximal level in the batch process. Prolonged electrolysis reduced chlorine concentration in the electrolyte and was detrimental to electrolysis efficiency, especially under high electric potential conditions. Therefore, the optimal choice of electrolysis time depends on the electrolyzable chloride in DOW and cell potential adopted for electrolysis. The higher the electric potential, the faster the chlorine level reaches its maximum, but the lower the electric efficiency will be.

  2. Effects of electrolysis time and electric potential on chlorine generation of electrolyzed deep ocean water.

    Science.gov (United States)

    Hsu, Guoo-Shyng Wang; Lu, Yi-Fa; Hsu, Shun-Yao

    2017-10-01

    Electrolyzed water is a sustainable disinfectant, which can comply with food safety regulations and is environmentally friendly. A two-factor central composite design was adopted for studying the effects of electrolysis time and electric potential on the chlorine generation efficiency of electrolyzed deep ocean water (DOW). DOW was electrolyzed in a glass electrolyzing cell equipped with platinum-plated titanium anode and cathode. The results showed that chlorine concentration reached maximal level in the batch process. Prolonged electrolysis reduced chlorine concentration in the electrolyte and was detrimental to electrolysis efficiency, especially under high electric potential conditions. Therefore, the optimal choice of electrolysis time depends on the electrolyzable chloride in DOW and cell potential adopted for electrolysis. The higher the electric potential, the faster the chlorine level reaches its maximum, but the lower the electric efficiency will be. Copyright © 2016. Published by Elsevier B.V.

  3. Measuring of Electric Potentials for the Diagnostics of Corrosion Protection of the Metal Structures

    National Research Council Canada - National Science Library

    R. M. Dzhala; B. Ya. Verbenets; M. I. Melnyk

    2016-01-01

    .... The structure and principles of operation of the new equipment aimed at measuring the constant and alternative electric voltages and at finding the polarization potential with removing the Ohmic component are described...

  4. Electric potential measured, concentration reported: how to get mmols from mV.

    Science.gov (United States)

    Lewenstam, A

    1996-01-01

    The interdependence between the electric potential of a galvanic cell and concentration of electrolytes will be analyzed in order to show how a clinically relevant report can be obtained. The use of SI units is emphasized.

  5. Nanometer-Scale Electrical Potential Profiling Across Perovskite Solar Cells

    Energy Technology Data Exchange (ETDEWEB)

    Xiao, Chuanxiao; Jiang, Chun-Sheng; Ke, Weijun; Wang, Changlei; Gorman, Brian; Yan, Yanfa; Al-Jassim, Mowafak

    2016-11-21

    We used Kelvin probe force microscopy to study the potential distribution on cross-section of perovskite solar cells with different types of electron-transporting layers (ETLs). Our results explain the low open-circuit voltage and fill factor in ETL-free cells, and support the fact that intrinsic SnO2 as an alternative ETL material can make high-performance devices. Furthermore, the potential-profiling results indicate a reduction in junction-interface recombination by the optimized SnO2 layer and adding a fullerene layer, which is consistent with the improved device performance and current-voltage hysteresis.

  6. Structure and functions of fungal cell surfaces

    Science.gov (United States)

    Nozawa, Y.

    1984-01-01

    A review with 24 references on the biochemistry, molecular structure, and function of cell surfaces of fungi, especially dermatophytes: the chemistry and structure of the cell wall, the effect of polyene antibiotics on the morphology and function of cytoplasmic membranes, and the chemical structure and function of pigments produced by various fungi are discussed.

  7. Swiss hydropower production. Optimisation potentials in European electricity trading; Die Schweizer Wasserkrafterzeugung. Optimierungspotenziale im europaeischen Stromhandel

    Energy Technology Data Exchange (ETDEWEB)

    Pieper, Thomas; Laue, Dirk Simon [RWE Supply and Trading GmbH, Essen (Germany)

    2011-10-15

    Swiss hydropower plays a central role in the formation of electricity prices in Western Europe as well as in cross-border electricity transmission. Although subject to constraints relating to hydrology as well as energy economy, its inherent flexibility makes it possible to make use of the most profitable electricity price differences relative to neighbouring European electricity markets (referred to as location spreads), especially between France and Italy. In times of favourable framework conditions it is also possible to use location spread potentials in relation to Germany. Studies in electricity statistics permit analysis of cross-border transmission schedules and spot price differences as well as identification of behavioural patterns in history and thus of optimisation potentials of Swiss hydropower production.

  8. Influence of the Ambient Electric Field on Measurements of the Actively Controlled Spacecraft Potential by MMS

    Science.gov (United States)

    Torkar, K.; Nakamura, R.; Andriopoulou, M.; Giles, B. L.; Jeszenszky, H.; Khotyaintsev, Y. V.; Lindqvist, P.-A.; Torbert, R. B.

    2017-12-01

    Space missions with sophisticated plasma instrumentation such as Magnetospheric Multiscale, which employs four satellites to explore near-Earth space benefit from a low electric potential of the spacecraft, to improve the plasma measurements and therefore carry instruments to actively control the potential by means of ion beams. Without control, the potential varies in anticorrelation with plasma density and temperature to maintain an equilibrium between the plasma current and the one of photoelectrons produced at the surface and overcoming the potential barrier. A drawback of the controlled, almost constant potential is the difficulty to use it as convenient estimator for plasma density. This paper identifies a correlation between the spacecraft potential and the ambient electric field, both measured by double probes mounted at the end of wire booms, as the main responsible for artifacts in the potential data besides the known effect of the variable photoelectron production due to changing illumination of the surface. It is shown that the effect of density variations is too weak to explain the observed correlation with the electric field and that a correction of the artifacts can be achieved to enable the reconstruction of the uncontrolled potential and plasma density in turn. Two possible mechanisms are discussed: the asymmetry of the current-voltage characteristic determining the probe to plasma potential and the fact that a large equipotential structure embedded in an electric field results in asymmetries of both the emission and spatial distribution of photoelectrons, which results in an increase of the spacecraft potential.

  9. Potential of electric vehicles in the public transport sector. Paper No. 7789

    Energy Technology Data Exchange (ETDEWEB)

    Jordan, J.

    1977-01-01

    In order to demonstrate the practicality of the high performance battery electric in urban areas, South Yorkshire Passenger Transport Executive has undertaken an extensive evaluation program. The operational responsibility encompasses the cities of Sheffield, Rotherham, Barnsley and Doncaster within the British heavy industrial and steel belt. All four British battery electric buses, of differing design concepts, are being utilized. They are the Chloride, Silent Rider, the Lucas Electric Midibus and two electric buses of similar size to the Lucas bus built by Crompton Limited in Wales. An assessment was made of the potential of battery driven vehicles in the passenger transit sector. Electric buses were capable of meeting the performance demands of existing timetabling on busy urban routes within the South Yorkshire Metropolitan area.

  10. Electrically configurable graphene field-effect transistors with a graded-potential gate.

    Science.gov (United States)

    Wang, Xiaowei; Jiang, Xingbin; Wang, Ting; Shi, Jia; Liu, Mingju; Zeng, Qibin; Cheng, Zhihai; Qiu, Xiaohui

    2015-05-13

    A device architecture for electrically configurable graphene field-effect transistor (GFET) using a graded-potential gate is present. The gating scheme enables a linearly varying electric field that modulates the electronic structure of graphene and causes a continuous shift of the Dirac points along the channel of GFET. This spatially varying electrostatic modulation produces a pseudobandgap observed as a suppressed conductance of graphene within a controllable energy range. By tuning the electrical gradient of the gate, a GFET device is reversibly transformed between ambipolar and n- and p-type unipolar characteristics. We further demonstrate an electrically programmable complementary inverter, showing the extensibility of the proposed architecture in constructing logic devices based on graphene and other Dirac materials. The electrical configurable GFET might be explored for novel functionalities in smart electronics.

  11. A physics-based potential and electric field model of a nanoscale ...

    Indian Academy of Sciences (India)

    In this paper, we have developed a physics-based model for surface potential, channel potential, electric field and drain current for AlGaN/GaN high electron mobility transistor with high-K gate dielectric using two-dimensional Poisson equation under full depletion approximation with the inclusion of effect of polarization ...

  12. An alternative approach to the electric charge quantization with non-global potentials

    Energy Technology Data Exchange (ETDEWEB)

    Rangel, Fabricio Augusto Barone; Silva, Giancarlo Thales Camilo da, E-mail: fabricio.barone@gmail.com, E-mail: gcamilo@unifei.edu.br [ICE, Universidade Federal de Itajuba, MG (Brazil); Helayel-Neto, Jose Abdalla, E-mail: helayel@cbpf.br [Centro Brasileiro de Pesquisas Fisicas (CBPF), Rio de Janeiro, RJ (Brazil)

    2012-07-01

    We present an alternative approach to justify the electric charge quantization by means of non-global electromagnetic potentials. We adopt a non-global potential whose singularity does not goes over to infinity and can be entirely embedded into an arbitrarily small closed ball. (author)

  13. Modelling the potential for wind energy integration on China’s coal-heavy electricity grid

    Science.gov (United States)

    Davidson, Michael R.; Zhang, Da; Xiong, Weiming; Zhang, Xiliang; Karplus, Valerie J.

    2016-07-01

    Expanding the use of wind energy for electricity generation forms an integral part of China’s efforts to address degraded air quality and climate change. However, the integration of wind energy into China’s coal-heavy electricity system presents significant challenges owing to wind’s variability and the grid’s system-wide inflexibilities. Here we develop a model to predict how much wind energy can be generated and integrated into China’s electricity mix, and estimate a potential production of 2.6 petawatt-hours (PWh) per year in 2030. Although this represents 26% of total projected electricity demand, it is only 10% of the total estimated physical potential of wind resources in the country. Increasing the operational flexibility of China’s coal fleet would allow wind to deliver nearly three-quarters of China’s target of producing 20% of primary energy from non-fossil sources by 2030.

  14. Non-invasive electrocardiogram detection of in vivo zebrafish embryos using electric potential sensors

    Science.gov (United States)

    Rendon-Morales, E.; Prance, R. J.; Prance, H.; Aviles-Espinosa, R.

    2015-11-01

    In this letter, we report the continuous detection of the cardiac electrical activity in embryonic zebrafish using a non-invasive approach. We present a portable and cost-effective platform based on the electric potential sensing technology, to monitor in vivo electrocardiogram activity from the zebrafish heart. This proof of principle demonstration shows how electrocardiogram measurements from the embryonic zebrafish may become accessible by using electric field detection. We present preliminary results using the prototype, which enables the acquisition of electrophysiological signals from in vivo 3 and 5 days-post-fertilization zebrafish embryos. The recorded waveforms show electrocardiogram traces including detailed features such as QRS complex, P and T waves.

  15. Kinetic Model of Electric Potentials in Localized Collisionless Plasma Structures under Steady Quasi-gyrotropic Conditions

    Science.gov (United States)

    Schindler, K.; Birn, J.; Hesse, M.

    2012-01-01

    Localized plasma structures, such as thin current sheets, generally are associated with localized magnetic and electric fields. In space plasmas localized electric fields not only play an important role for particle dynamics and acceleration but may also have significant consequences on larger scales, e.g., through magnetic reconnection. Also, it has been suggested that localized electric fields generated in the magnetosphere are directly connected with quasi-steady auroral arcs. In this context, we present a two-dimensional model based on Vlasov theory that provides the electric potential for a large class of given magnetic field profiles. The model uses an expansion for small deviation from gyrotropy and besides quasineutrality it assumes that electrons and ions have the same number of particles with their generalized gyrocenter on any given magnetic field line. Specializing to one dimension, a detailed discussion concentrates on the electric potential shapes (such as "U" or "S" shapes) associated with magnetic dips, bumps, and steps. Then, it is investigated how the model responds to quasi-steady evolution of the plasma. Finally, the model proves useful in the interpretation of the electric potentials taken from two existing particle simulations.

  16. Kinetic model of electric potentials in localized collisionless plasma structures under steady quasi-gyrotropic conditions

    Energy Technology Data Exchange (ETDEWEB)

    Schindler, K. [Ruhr University Bochum, 44780 Bochum (Germany); Birn, J. [Space Science Institute, Boulder, Colorado 80301 (United States); Hesse, M. [Nasa Goddard Space Flight Center, Greenbelt, Maryland 20771 (United States)

    2012-08-15

    Localized plasma structures, such as thin current sheets, generally are associated with localized magnetic and electric fields. In space plasmas localized electric fields not only play an important role for particle dynamics and acceleration but may also have significant consequences on larger scales, e.g., through magnetic reconnection. Also, it has been suggested that localized electric fields generated in the magnetosphere are directly connected with quasi-steady auroral arcs. In this context, we present a two-dimensional model based on Vlasov theory that provides the electric potential for a large class of given magnetic field profiles. The model uses an expansion for small deviation from gyrotropy and besides quasineutrality it assumes that electrons and ions have the same number of particles with their generalized gyrocenter on any given magnetic field line. Specializing to one dimension, a detailed discussion concentrates on the electric potential shapes (such as 'U' or 'S' shapes) associated with magnetic dips, bumps, and steps. Then, it is investigated how the model responds to quasi-steady evolution of the plasma. Finally, the model proves useful in the interpretation of the electric potentials taken from two existing particle simulations.

  17. Enhancing the stability of xylanase from Cellulomonas fimi by cell-surface display on Escherichia coli.

    Science.gov (United States)

    Chen, Y-P; Hwang, I-E; Lin, C-J; Wang, H-J; Tseng, C-P

    2012-03-01

    The cell-surface display of Cex, which encodes xylanase and exoglucanase from Cellulomonas fimi, was constructed on Escherichia coli using PgsA as the anchor protein. Characterization of the cell-surface display of Cex was performed. PgsA was fused to the N-terminus of Cex and six histidines were utilized as spacers between the targeting and anchor proteins. Successful cell-surface display of Cex was demonstrated by Western blot and immunofluorescence analyses on E. coli C41 (DE3). According to the time-course analysis, the xylanase activity of Cex was achieved at 49Ug(-1) dry cell weight after 12 h culture at 37°C. The optimal temperature and pH ranges of the cell-surface displayed protein with whole-cell were broader than the corresponding ranges of the purified form. Further determination of thermostability indicated that the half-life of cell-surface displayed Cex was 1·6 times longer than that of purified Cex at 60°C. We have successfully developed the cell-surface display of xylanase on E. coli. The cell-surface display can enhance the stability of xylanase against changes in temperature and has the potential of becoming a whole-cell biocatalyst for industrial applications, such as biobleaching of paper and production of renewable energy. The results demonstrated that the cell-surface display of xylanase embedded in the cell membrane is more stable than that of the purified enzyme. Thus, to improve the stability of heterologous proteins production, cell-surface display using the PgsA anchor protein as a tool can be considered in E. coli. © 2012 The Authors. Journal of Applied Microbiology © 2012 The Society for Applied Microbiology.

  18. The potential and electric field in the cochlear outer hair cell membrane.

    Science.gov (United States)

    Harland, Ben; Lee, Wen-han; Brownell, William E; Sun, Sean X; Spector, Alexander A

    2015-05-01

    Outer hair cell electromechanics, critically important to mammalian active hearing, is driven by the cell membrane potential. The membrane protein prestin is a crucial component of the active outer hair cell's motor. The focus of the paper is the analysis of the local membrane potential and electric field resulting from the interaction of electric charges involved. Here the relevant charges are the ions inside and outside the cell, lipid bilayer charges, and prestin-associated charges (mobile-transferred by the protein under the action of the applied field, and stationary-relatively unmoved by the field). The electric potentials across and along the membrane are computed for the case of an applied DC-field. The local amplitudes and phases of the potential under different frequencies are analyzed for the case of a DC + AC-field. We found that the effect of the system of charges alters the electric potential and internal field, which deviate significantly from their traditional linear and constant distributions. Under DC + AC conditions, the strong frequency dependence of the prestin mobile charge has a relatively small effect on the amplitude and phase of the resulting potential. The obtained results can help in a better understanding and experimental verification of the mechanism of prestin performance.

  19. Non-uniform distribution of outer hair cell transmembrane potential induced by extracellular electric field.

    Science.gov (United States)

    Ramamoorthy, Sripriya; Wilson, Teresa M; Wu, Tao; Nuttall, Alfred L

    2013-12-17

    Intracochlear electric fields arising out of sound-induced receptor currents, silent currents, or electrical current injected into the cochlea induce transmembrane potential along the outer hair cell (OHC) but its distribution along the cells is unknown. In this study, we investigated the distribution of OHC transmembrane potential induced along the cell perimeter and its sensitivity to the direction of the extracellular electric field (EEF) on isolated OHCs at a low frequency using the fast voltage-sensitive dye ANNINE-6plus. We calibrated the potentiometric sensitivity of the dye by applying known voltage steps to cells by simultaneous whole-cell voltage clamp. The OHC transmembrane potential induced by the EEF is shown to be highly nonuniform along the cell perimeter and strongly dependent on the direction of the electrical field. Unlike in many other cells, the EEF induces a field-direction-dependent intracellular potential in the cylindrical OHC. We predict that without this induced intracellular potential, EEF would not generate somatic electromotility in OHCs. In conjunction with the known heterogeneity of OHC membrane microdomains, voltage-gated ion channels, charge, and capacitance, the EEF-induced nonuniform transmembrane potential measured in this study suggests that the EEF would impact the cochlear amplification and electropermeability of molecules across the cell. Copyright © 2013 Biophysical Society. Published by Elsevier Inc. All rights reserved.

  20. Numerical and experimental study of the effect of the induced electric potential in Lorentz force velocimetry

    Science.gov (United States)

    Hernández, Daniel; Boeck, Thomas; Karcher, Christian; Wondrak, Thomas

    2018-01-01

    Lorentz force velocimetry (LFV) is a contactless velocity measurement technique for electrically conducting fluids. When a liquid metal or a molten glass flows through an externally applied magnetic field, eddy currents and a flow-braking force are generated inside the liquid. This force is proportional to the velocity or flow rate of the fluid and, due to Newton’s third law, a force of the same magnitude but in opposite direction acts on the source of the applied magnetic field which in our case are permanent magnets. According to Ohm’s law for moving conductors at low magnetic Reynolds numbers, an electric potential is induced which ensures charge conservation. In this paper, we analyze the contribution of the induced electric potential to the total Lorentz force by considering two different scenarios: conducting walls of finite thickness and aspect ratio variation of the cross-section of the flow. In both the cases, the force component generated by the electric potential is always in the opposite direction to the total Lorentz force. This force component is sensitive to the electric boundary conditions of the flow of which insulating and perfectly conducting walls are the two limiting cases. In the latter case, the overall electric resistance of the system is minimized, resulting in a considerable increase in the measured Lorentz force. Additionally, this force originating from the electric potential also decays when the aspect ratio of the cross-section of the flow is changed. Hence, the sensitivity of the measurement technique is enhanced by either increasing wall conductivity or optimizing the aspect ratio of the cross-section of the flow.

  1. Cell surface analysis and adhesion of chemically modified streptococci

    NARCIS (Netherlands)

    van der Mei, HC; van de Belt-Gritter, B; Doyle, RJ; Busscher, HJ

    2001-01-01

    In this paper, streptococcal cell surfaces are chemically modified, and the effects of the modification on cell surface hydrophobicity and charge, together with adhesion to hexadecane are determined. Acetic and succinic anhydrides, neutralizing or converting ammonium groups into negatively charged

  2. Comparison of three methods for the estimation of cross-shock electric potential using Cluster data

    Directory of Open Access Journals (Sweden)

    A. P. Dimmock

    2011-05-01

    Full Text Available Cluster four point measurements provide a comprehensive dataset for the separation of temporal and spatial variations, which is crucial for the calculation of the cross shock electrostatic potential using electric field measurements. While Cluster is probably the most suited among present and past spacecraft missions to provide such a separation at the terrestrial bow shock, it is far from ideal for a study of the cross shock potential, since only 2 components of the electric field are measured in the spacecraft spin plane. The present paper is devoted to the comparison of 3 different techniques that can be used to estimate the potential with this limitation. The first technique is the estimate taking only into account the projection of the measured components onto the shock normal. The second uses the ideal MHD condition E·B = 0 to estimate the third electric field component. The last method is based on the structure of the electric field in the Normal Incidence Frame (NIF for which only the potential component along the shock normal and the motional electric field exist. All 3 approaches are used to estimate the potential for a single crossing of the terrestrial bow shock that took place on the 31 March 2001. Surprisingly all three methods lead to the same order of magnitude for the cross shock potential. It is argued that the third method must lead to more reliable results. The effect of the shock normal inaccuracy is investigated for this particular shock crossing. The resulting electrostatic potential appears too high in comparison with the theoretical results for low Mach number shocks. This shows the variability of the potential, interpreted in the frame of the non-stationary shock model.

  3. Cortical potentials after electrical intraneural stimulation of the optic nerve during orbital enucleation.

    Science.gov (United States)

    Benedičič, Mitja; Beltram, Matej; Olup, Brigita Drnovšek; Bošnjak, Roman

    2012-12-01

    The aim of this study was to present cortical potentials after electrical intraneural stimulation of the optic nerve during orbital enucleation due to malignant melanoma of the choroid or the ciliary body. These cortical potentials were related to cortical potentials after electrical epidural stimulation of the optic nerve, recorded during non-manipulative phases of neurosurgery for central skull base tumors. Cortical potentials were recorded with surface occipital electrode (Oz) in six patients undergoing orbital enucleation under total intravenous anesthesia. Two thin needle stimulating electrodes were inserted inside the intraorbital part of the optic nerve. The electrical stimulus consisted of a rectangular current pulse of varying intensity (0.2-10.0 mA) and duration (0.1-0.3 ms); the stimulation rate was 2 Hz; the bandpass filter was 1-1,000 Hz; the analysis time was 50-300 ms. Cortical potentials could not be obtained or were inconsistently elicitable in three patients with longstanding history (>3 months) of severe visual deterioration, while they consisted of several positive and negative deflections in a patient with a short history of mild visual impairment. In two other patients, cortical potentials consisted of N20, P30 and N40 waves. Cortical potentials after electrical intraneural stimulation of the optic nerve could be recorded in patients with a short history of visual deterioration and without optic nerve atrophy and appear more heterogeneous than cortical potentials after electrical epidural stimulation of the optic nerve, recorded during non-manipulative phases of neurosurgery for central skull base tumors.

  4. Comparison of three methods for the estimation of cross-shock electric potential using Cluster data

    Directory of Open Access Journals (Sweden)

    Y. Hobara

    2011-05-01

    Full Text Available Cluster four point measurements provide a comprehensive dataset for the separation of temporal and spatial variations, which is crucial for the calculation of the cross shock electrostatic potential using electric field measurements. While Cluster is probably the most suited among present and past spacecraft missions to provide such a separation at the terrestrial bow shock, it is far from ideal for a study of the cross shock potential, since only 2 components of the electric field are measured in the spacecraft spin plane. The present paper is devoted to the comparison of 3 different techniques that can be used to estimate the potential with this limitation. The first technique is the estimate taking only into account the projection of the measured components onto the shock normal. The second uses the ideal MHD condition E·B = 0 to estimate the third electric field component. The last method is based on the structure of the electric field in the Normal Incidence Frame (NIF for which only the potential component along the shock normal and the motional electric field exist. All 3 approaches are used to estimate the potential for a single crossing of the terrestrial bow shock that took place on the 31 March 2001. Surprisingly all three methods lead to the same order of magnitude for the cross shock potential. It is argued that the third method must lead to more reliable results. The effect of the shock normal inaccuracy is investigated for this particular shock crossing. The resulting electrostatic potential appears too high in comparison with the theoretical results for low Mach number shocks. This shows the variability of the potential, interpreted in the frame of the non-stationary shock model.

  5. On the quasi-polynomial 3D potentials of electric and magnetic fields

    Directory of Open Access Journals (Sweden)

    Nadezhda K. Krasnova

    2017-03-01

    Full Text Available Spectrographic electron and ion optical structures markedly raise the possibilities of modern energy and mass analysis. Electric and magnetic fields which potentials are expressed by functions homogeneous in Euler's sense are the effective instrumentation that is used for creating new spectrographic analytical devices with the determined working characteristics. This paper puts forward and discusses some methods for building 3D harmonic and homogeneous in Euler's sense structures representable as the polynomials of finite degree with respect to one of variables. These strictly mathematical approaches provide a possibility of expanding significantly a class of quasi-polynomial potentials and of enriching modern analytical instrumentation by new spectrographic electrical and magnetic configurations.

  6. Direct Measurement of Built-in Electrical Potential in Photovoltaic Devices by Scanning Kelvin Probe Microscopy

    Energy Technology Data Exchange (ETDEWEB)

    Jiang, C. S.; Mutinho, H. R.; Hasoon, F. S.; Al-Thani, H. A.; Friedman, D. J.; Geisz, J. F.; Wang, Q.; Romero, M. J.; Al-Jassim, M. M.

    2003-05-01

    We report on direct measurements of the built-in electrical potential in Cu(In,Ga)Se2, GaInP2 single-junction, and GaInP2/GaAs tandem-junction solar cells, by using scanning Kelvin probe microscopy. Potential profiles on cross sections of the devices were measured quantitatively and spatially resolved in open and short circuit, under and without illuminations, with selective photon energies matching the band gaps of the junctions. The measurements provide valuable information about the electrical properties of the devices and are useful for understanding the performance and improving the design of solar cells.

  7. Probes for anionic cell surface detection

    Science.gov (United States)

    Smith, Bradley D.

    2013-03-05

    Embodiments of the present invention are generally directed to compositions comprising a class of molecular probes for detecting the presence of anionic cell surfaces. Embodiments include compositions that are enriched for these compositions and preparations, particularly preparations suitable for use as laboratory/clinical reagents and diagnostic indicators, either alone or as part of a kit. An embodiment of the invention provides for a highly selective agent useful in the discernment and identification of dead or dying cells, such as apoptotic cells, in a relatively calcium-free environment. An embodiment of the invention provides a selective agent for the identification of bacteria in a mixed population of bacterial cells and nonbacterial cells.

  8. An economic analysis of the electricity generation potential from biogas resources in the state of Indiana

    Science.gov (United States)

    Giraldo, Juan S.

    Anaerobic digestion is a process that is a common part of organic waste management systems and is used in concentrated animal feeding operations (CAFOs), wastewater treatment plants (WWTPs), and municipal solid waste (MSW) landfills. The process produces biogas, which contains methane, and it can be burned to generate electricity. Previous reports have indicated that based on the availability of feedstocks there is a large potential for biogas production and use for electricity generation in the state of Indiana. However, these reports varied in their consideration of important factors that affect the technical and economic feasibility of being able to develop the resources available. The goal of this thesis is to make a more targeted assessment of the electricity generation potential from biogas resources at CAFOs, WWTPs, and MSW landfills in Indiana. A capital budgeting model is used to estimate the net present value (NPV) of biogas electricity projects at facilities that are identified as technically suitable. A statewide estimate of the potential generation capacity is made by estimating the number of facilities that could profitably undertake a biogas electricity project. In addition this thesis explored the impact that different incentive policies would have on the economic viability of these projects. The results indicated that the electricity generation potential is much smaller when technical and economic factors are taken into account in addition to feedstock availability. In particular it was found that projects at hog farms are unlikely to be economically feasible in the present even when financial incentives are considered. In total, 47.94 MW of potential generating capacity is estimated from biogas production at CAFOs, WWTPs, and MSW landfills. Though results indicated that 37.10 MW of capacity are economically feasible under current operating conditions, sensitivity analysis reveals that these projects are very sensitive to capital cost assumptions

  9. A novel electrical potential sensing method for in vitro stent fracture monitoring and detection.

    Science.gov (United States)

    Park, Chan-Hee; Tijing, Leonard D; Yun, Yeoheung; Kim, Cheol Sang

    2011-01-01

    This article describes a preliminary investigation and prototype fabrication of a novel potential sensing method to continuously monitor vascular stent fractures. A potential measurement system consisting of Wheatstone bridge circuit and signal conditioning circuit was designed for the cardiovascular stent durability and fatigue test. Each end of a bare and polyurethane-covered Nitinol vascular stent was electrically connected to the potential measurement system and then immersed either in simulated body fluid (SBF) media or distilled water at 36.4 ± 1 °C. When the stent experienced fracture (i.e., a cut), its electrical potential decreased with an increase in electrical resistance. This method successfully measured fractures in the stent regardless of location. Furthermore, the number of cycles at the onset of stent fracture was accurately detected and continuously monitored using this technique. Thus, the present fracture detection method, which to our knowledge is the first ever report to use electrical potential measurement for stent durability test, gives a fast, real-time, accurate and efficient detection of fractures in stent during in vitro fatigue and durability test.

  10. Cortical somatosensory evoked potentials from lumbosacral dermatomes: airpuff versus electrical stimulation.

    Science.gov (United States)

    Schimsheimer, R J; Boejharat, K R; van der Sluijs, J C; Stijnen, T; Gryz, E

    1995-01-01

    Cortical potentials were elicited by airpuff stimulation of the L5 and S1 dermatome in a group of 24 healthy volunteers. The results were compared with the SEPs obtained by conventional electrical stimulation. Both stimulus modalities produce stable and good reproducible cortical responses of similar waveform. The most stable second negative peak, labeled N2, was used in this study. Mean latencies (in msec) were: N2 L5 air = 67.1 +/- 3.3, N2 L5 electr. = 55.7 +/- 3.7 N2 S1 air = 67.2 +/- 3.9, and N2 S1 electr. = 55.1 +/- 2.9 The maximum R/L difference (mean + 3 SD) was 5.7 msec, 5.9 msec, 7.2 msec and 7.2 msec for respectively N2 L5 air, N2 L5 electrical, N2 S1 air and N2 S1 electrical. Single regression analysis showed a significant influence of height, but not age upon all latencies. Multiple regression analysis with height and age as independent variables showed a significant influence of height and age together upon the latencies of the electrical SEP (both L5 and S1). For the airpuff SEP only height was significant. Gender had no effect on the cortical components. The amplitude of peak N2 after electrical stimulation of the S1 dermatome was significant higher than after airpuff stimulation, 2.9 and 1.7 microvolt respectively. For the L5 dermatome both types of stimuli produced responses of nearly equal amplitude, 2.5 and 2.1 microvolt for electrical and airpuff stimulation respectively. Airpuff SEPs may provide a good alternative for electrical stimulation.

  11. Evaluation of potential opportunities for electric power generation from landfill gas at “Tsalapitsa”

    Directory of Open Access Journals (Sweden)

    Ganev Ivaylo

    2014-01-01

    Full Text Available Potential opportunities for electric power generation from landfill gas (LFG utilization were estimated for the second largest landfill site in Bulgaria, situated near the city of Plovdiv. The work performed was based on detailed analysis of experimentally obtained and model-predicted features of the “Tsalapitsa” landfill site. The study presents a short description of the site, the global characteristics of the disposed municipal solid waste, and the experimentally obtained methane composition of the LFG. Based on the above described observations, the potential for LFG recovery at “Tsalapitsa” was determined, together with that for electric power generation for the next 25 years. A set of recommendations was then developed regarding the parameters required for the installation of electric power generation from LFG in Plovdiv.

  12. Effects on functional groups and zeta potential of SAP1pulsed electric field technology.

    Science.gov (United States)

    Liang, Rong; Li, Xuenan; Lin, Songyi; Wang, Jia

    2017-01-01

    SAP 1 pulsed electric field (PEF) technology. The effects of electric field intensity and pulse frequency on SAP 1 electric field intensity 15 kV cm -1 , pulse frequency 1600 Hz and flow velocity 2.93 mL min -1 ). Furthermore, the PEF-treated SAP 1 < MW < 3kDa under optimal conditions lacked the characteristic absorbance of N-H, C = C and the amide band and the zeta potential was reduced to -18.0 mV. Overall, the results of the present study suggest that the improvement of antioxidant activity of SAP 1 < MW < 3kDa is a result of the contribution of the functional groups and the change in zeta potential when treated with PEF. © 2016 Society of Chemical Industry. © 2016 Society of Chemical Industry.

  13. DSM Electricity Savings Potential in the Buildings Sector in APP Countries

    Energy Technology Data Exchange (ETDEWEB)

    McNeil, MIchael; Letschert, Virginie; Shen, Bo; Sathaye, Jayant; de la Ru du Can, Stephane

    2011-01-12

    The global economy has grown rapidly over the past decade with a commensurate growth in the demand for electricity services that has increased a country's vulnerability to energy supply disruptions. Increasing need of reliable and affordable electricity supply is a challenge which is before every Asia Pacific Partnership (APP) country. Collaboration between APP members has been extremely fruitful in identifying potential efficiency upgrades and implementing clean technology in the supply side of the power sector as well established the beginnings of collaboration. However, significantly more effort needs to be focused on demand side potential in each country. Demand side management or DSM in this case is a policy measure that promotes energy efficiency as an alternative to increasing electricity supply. It uses financial or other incentives to slow demand growth on condition that the incremental cost needed is less than the cost of increasing supply. Such DSM measures provide an alternative to building power supply capacity The type of financial incentives comprise of rebates (subsidies), tax exemptions, reduced interest loans, etc. Other approaches include the utilization of a cap and trade scheme to foster energy efficiency projects by creating a market where savings are valued. Under this scheme, greenhouse gas (GHG) emissions associated with the production of electricity are capped and electricity retailers are required to meet the target partially or entirely through energy efficiency activities. Implementation of DSM projects is very much in the early stages in several of the APP countries or localized to a regional part of the country. The purpose of this project is to review the different types of DSM programs experienced by APP countries and to estimate the overall future potential for cost-effective demand-side efficiency improvements in buildings sectors in the 7 APP countries through the year 2030. Overall, the savings potential is estimated to be

  14. Retrieving electric resistivity data from self-potential measurements by cross-correlation

    NARCIS (Netherlands)

    Slob, E.C.; Snieder, R.; Revil, A.

    2010-01-01

    We show that the two-point cross-correlation of self-potential field recordings is equal to the electric resistivity between the two points. This holds under the condition that spatially and temporally uncorrelated noise sources exist throughout the volume. These sources should have a known

  15. Electric-double-layer potential distribution in multiple-layer immiscible electrolytes

    NARCIS (Netherlands)

    Das, S.; Hardt, Steffen

    2011-01-01

    In this Brief Report, we calculate the electric-double-layer (EDL) electrostatic potential in a system of several layers of immiscible electrolytes. Verwey-Niessen theory predicts that at the interface between two immiscible electrolytes back-to-back EDLs are formed. The present analysis extends

  16. Tension moderation and fluctuation spectrum in simulated lipid membranes under an applied electric potential

    DEFF Research Database (Denmark)

    Loubet, Bastien; Lomholt, Michael Andersen; Khandelia, Himanshu

    2013-01-01

    We investigate the effect of an applied electric potential on the mechanics of a coarse grained POPC bilayer under tension. The size and duration of our simulations allow for a detailed and accurate study of the fluctuations. Effects on the fluctuation spectrum, tension, bending rigidity, and bil......We investigate the effect of an applied electric potential on the mechanics of a coarse grained POPC bilayer under tension. The size and duration of our simulations allow for a detailed and accurate study of the fluctuations. Effects on the fluctuation spectrum, tension, bending rigidity......, and bilayer thickness are investigated in detail. In particular, the least square fitting technique is used to calculate the fluctuation spectra. The simulations confirm a recently proposed theory that the effect of an applied electric potential on the membrane will be moderated by the elastic properties...... fluctuations. The effect of the applied electric potential on the bending rigidity is non-existent within error bars. However, when the membrane is stretched there is a point where the bending rigidity is lowered due to a decrease of the thickness of the membrane. All these effects should prove important...

  17. Survey of wind power potential for wind-based electricity at ...

    African Journals Online (AJOL)

    The potential for wind-generated electricity is examined using 22 months wind data collected from a prospective site located in the southern highlands of Tanzania. While the data for the year 2001 was from March to December that of 2002 was for all the twelve months of the year. Characteristics of monthly and annual wind ...

  18. Nociception-related somatosensory evoked potentials in awake dogs recorded after intra epidermal electrical stimulation

    NARCIS (Netherlands)

    van Oostrom, H.; Stienen, P.J.; Doornenbal, A.; Hellebrekers, L.J.

    2009-01-01

    Eur J Pain. 2009 Feb;13(2):154-60. Epub 2008 May 16. Nociception-related somatosensory evoked potentials in awake dogs recorded after intra epidermal electrical stimulation. van Oostrom H, Stienen PJ, Doornenbal A, Hellebrekers LJ. Department of Clinical Sciences of Companion Animals, Division

  19. Transmembrane potential generated by a magnetically induced transverse electric field in a cylindrical axonal model.

    Science.gov (United States)

    Ye, Hui; Cotic, Marija; Fehlings, Michael G; Carlen, Peter L

    2011-01-01

    During the electrical stimulation of a uniform, long, and straight nerve axon, the electric field oriented parallel to the axon has been widely accepted as the major field component that activates the axon. Recent experimental evidence has shown that the electric field oriented transverse to the axon is also sufficient to activate the axon, by inducing a transmembrane potential within the axon. The transverse field can be generated by a time-varying magnetic field via electromagnetic induction. The aim of this study was to investigate the factors that influence the transmembrane potential induced by a transverse field during magnetic stimulation. Using an unmyelinated axon model, we have provided an analytic expression for the transmembrane potential under spatially uniform, time-varying magnetic stimulation. Polarization of the axon was dependent on the properties of the magnetic field (i.e., orientation to the axon, magnitude, and frequency). Polarization of the axon was also dependent on its own geometrical (i.e., radius of the axon and thickness of the membrane) and electrical properties (i.e., conductivities and dielectric permittivities). Therefore, this article provides evidence that aside from optimal coil design, tissue properties may also play an important role in determining the efficacy of axonal activation under magnetic stimulation. The mathematical basis of this conclusion was discussed. The analytic solution can potentially be used to modify the activation function in current cable equations describing magnetic stimulation.

  20. Ion Permeability of Artificial Membranes Evaluated by Diffusion Potential and Electrical Resistance Measurements

    Science.gov (United States)

    Shlyonsky, Vadim

    2013-01-01

    In the present article, a novel model of artificial membranes that provides efficient assistance in teaching the origins of diffusion potentials is proposed. These membranes are made of polycarbonate filters fixed to 12-mm plastic rings and then saturated with a mixture of creosol and "n"-decane. The electrical resistance and potential…

  1. A study of wind energy potential: remedy for fluctuation of electric ...

    African Journals Online (AJOL)

    Aside, wind energy is a renewable source of energy and it is environmentally friendly which conforms to the national energy and environmental policy of Nigeria with respect to the new world order of economic development and environmental sustainability. Keywords: wind energy potential, electric power fluctuation, wind ...

  2. Students' Reasoning When Tackling Electric Field and Potential in Explanation of DC Resistive Circuits

    Science.gov (United States)

    Leniz, Ane; Zuza, Kristina; Guiasola, Jenaro

    2017-01-01

    This study examines the causal reasoning that university students use to explain how dc circuits work. We analyze how students use the concepts of electric field and potential difference in their explanatory models of dc circuits, and what kinds of reasoning they use at the macroscopic and microscopic levels in their explanations. This knowledge…

  3. Quantitative comparison of a human cancer cell surface proteome between interphase and mitosis.

    Science.gov (United States)

    Özlü, Nurhan; Qureshi, Mohammad H; Toyoda, Yusuke; Renard, Bernhard Y; Mollaoglu, Gürkan; Özkan, Nazlı E; Bulbul, Selda; Poser, Ina; Timm, Wiebke; Hyman, Anthony A; Mitchison, Timothy J; Steen, Judith A

    2015-01-13

    The cell surface is the cellular compartment responsible for communication with the environment. The interior of mammalian cells undergoes dramatic reorganization when cells enter mitosis. These changes are triggered by activation of the CDK1 kinase and have been studied extensively. In contrast, very little is known of the cell surface changes during cell division. We undertook a quantitative proteomic comparison of cell surface-exposed proteins in human cancer cells that were tightly synchronized in mitosis or interphase. Six hundred and twenty-eight surface and surface-associated proteins in HeLa cells were identified; of these, 27 were significantly enriched at the cell surface in mitosis and 37 in interphase. Using imaging techniques, we confirmed the mitosis-selective cell surface localization of protocadherin PCDH7, a member of a family with anti-adhesive roles in embryos. We show that PCDH7 is required for development of full mitotic rounding pressure at the onset of mitosis. Our analysis provided basic information on how cell cycle progression affects the cell surface. It also provides potential pharmacodynamic biomarkers for anti-mitotic cancer chemotherapy. © 2014 The Authors.

  4. Tuning coercive force by adjusting electric potential in solution processed Co/Pt(111) and the mechanism involved

    Science.gov (United States)

    Chang, Cheng-Hsun-Tony; Kuo, Wei-Hsu; Chang, Yu-Chieh; Tsay, Jyh-Shen; Yau, Shueh-Lin

    2017-03-01

    A combination of a solution process and the control of the electric potential for magnetism represents a new approach to operating spintronic devices with a highly controlled efficiency and lower power consumption with reduced production cost. As a paradigmatic example, we investigated Co/Pt(111) in the Bloch-wall regime. The depression in coercive force was detected by applying a negative electric potential in an electrolytic solution. The reversible control of coercive force by varying the electric potential within few hundred millivolts is demonstrated. By changing the electric potential in ferromagnetic layers with smaller thicknesses, the efficiency for controlling the tunable coercive force becomes higher. Assuming that the pinning domains are independent of the applied electric potential, an electric potential tuning-magnetic anisotropy energy model was derived and provided insights into our knowledge of the relation between the electric potential tuning coercive force and the thickness of the ferromagnetic layer. Based on the fact that the coercive force can be tuned by changing the electric potential using a solution process, we developed a novel concept of electric-potential-tuned magnetic recording, resulting in a stable recording media with a high degree of writing ability.

  5. Tuning coercive force by adjusting electric potential in solution processed Co/Pt(111) and the mechanism involved.

    Science.gov (United States)

    Chang, Cheng-Hsun-Tony; Kuo, Wei-Hsu; Chang, Yu-Chieh; Tsay, Jyh-Shen; Yau, Shueh-Lin

    2017-03-03

    A combination of a solution process and the control of the electric potential for magnetism represents a new approach to operating spintronic devices with a highly controlled efficiency and lower power consumption with reduced production cost. As a paradigmatic example, we investigated Co/Pt(111) in the Bloch-wall regime. The depression in coercive force was detected by applying a negative electric potential in an electrolytic solution. The reversible control of coercive force by varying the electric potential within few hundred millivolts is demonstrated. By changing the electric potential in ferromagnetic layers with smaller thicknesses, the efficiency for controlling the tunable coercive force becomes higher. Assuming that the pinning domains are independent of the applied electric potential, an electric potential tuning-magnetic anisotropy energy model was derived and provided insights into our knowledge of the relation between the electric potential tuning coercive force and the thickness of the ferromagnetic layer. Based on the fact that the coercive force can be tuned by changing the electric potential using a solution process, we developed a novel concept of electric-potential-tuned magnetic recording, resulting in a stable recording media with a high degree of writing ability.

  6. A class of Fourier integrals based on the electric potential of an elongated dipole.

    Science.gov (United States)

    Skianis, Georgios Aim

    2014-01-01

    In the present paper the closed expressions of a class of non tabulated Fourier integrals are derived. These integrals are associated with a group of functions at space domain, which represent the electric potential of a distribution of elongated dipoles which are perpendicular to a flat surface. It is shown that the Fourier integrals are produced by the Fourier transform of the Green's function of the potential of the dipole distribution, times a definite integral in which the distribution of the polarization is involved. Therefore the form of this distribution controls the expression of the Fourier integral. Introducing various dipole distributions, the respective Fourier integrals are derived. These integrals may be useful in the quantitative interpretation of electric potential anomalies produced by elongated dipole distributions, at spatial frequency domain.

  7. Biomolecular strategies for cell surface engineering

    Science.gov (United States)

    Wilson, John Tanner

    Islet transplantation has emerged as a promising cell-based therapy for the treatment of diabetes, but its clinical efficacy remains limited by deleterious host responses that underlie islet destruction. In this dissertation, we describe the assembly of ultrathin conformal coatings that confer molecular-level control over the composition and biophysicochemical properties of the islet surface with implications for improving islet engraftment. Significantly, this work provides novel biomolecular strategies for cell surface engineering with broad biomedical and biotechnological applications in cell-based therapeutics and beyond. Encapsulation of cells and tissue offers a rational approach for attenuating deleterious host responses towards transplanted cells, but a need exists to develop cell encapsulation strategies that minimize transplant volume. Towards this end, we endeavored to generate nanothin films of diverse architecture with tunable properties on the extracellular surface of individual pancreatic islets through a process of layer-by-layer (LbL) self assembly. We first describe the formation of poly(ethylene glycol) (PEG)-rich conformal coatings on islets via LbL self assembly of poly(L-lysine)-g-PEG(biotin) and streptavidin. Multilayer thin films conformed to the geometrically and chemically heterogeneous islet surface, and could be assembled without loss of islet viability or function. Significantly, coated islets performed comparably to untreated controls in a murine model of allogenic intraportal islet transplantation, and, to our knowledge, this is the first study to report in vivo survival and function of nanoencapsulated cells or cell aggregates. Based on these findings, we next postulated that structurally similar PLL-g-PEG copolymers comprised of shorter PEG grafts might be used to initiate and propagate the assembly of polyelectrolyte multilayer (PEM) films on pancreatic islets, while simultaneously preserving islet viability. Through control of PLL

  8. Actin cortex architecture regulates cell surface tension.

    Science.gov (United States)

    Chugh, Priyamvada; Clark, Andrew G; Smith, Matthew B; Cassani, Davide A D; Dierkes, Kai; Ragab, Anan; Roux, Philippe P; Charras, Guillaume; Salbreux, Guillaume; Paluch, Ewa K

    2017-06-01

    Animal cell shape is largely determined by the cortex, a thin actin network underlying the plasma membrane in which myosin-driven stresses generate contractile tension. Tension gradients result in local contractions and drive cell deformations. Previous cortical tension regulation studies have focused on myosin motors. Here, we show that cortical actin network architecture is equally important. First, we observe that actin cortex thickness and tension are inversely correlated during cell-cycle progression. We then show that the actin filament length regulators CFL1, CAPZB and DIAPH1 regulate mitotic cortex thickness and find that both increasing and decreasing thickness decreases tension in mitosis. This suggests that the mitotic cortex is poised close to a tension maximum. Finally, using a computational model, we identify a physical mechanism by which maximum tension is achieved at intermediate actin filament lengths. Our results indicate that actin network architecture, alongside myosin activity, is key to cell surface tension regulation.

  9. Electrical Potential of Acupuncture Points: Use of a Noncontact Scanning Kelvin Probe

    Directory of Open Access Journals (Sweden)

    Brian J. Gow

    2012-01-01

    Full Text Available Objective. Acupuncture points are reportedly distinguishable by their electrical properties. However, confounders arising from skin-to-electrode contact used in traditional electrodermal methods have contributed to controversies over this claim. The Scanning Kelvin Probe is a state-of-the-art device that measures electrical potential without actually touching the skin and is thus capable of overcoming these confounding effects. In this study, we evaluated the electrical potential profiles of acupoints LI-4 and PC-6 and their adjacent controls. We hypothesize that acupuncture point sites are associated with increased variability in potential compared to adjacent control sites. Methods. Twelve healthy individuals were recruited for this study. Acupuncture points LI-4 and PC-6 and their adjacent controls were assessed. A 2 mm probe tip was placed over the predetermined skin site and adjusted to a tip-to-sample distance of 1.0 mm under tip oscillation settings of 62.4 Hz frequency. A surface potential scan spanning a 1.0 cm × 1.0 cm area was obtained. Results. At both the PC-6 and LI-4 sites, no significant differences in mean potential were observed compared to their respective controls (Wilcoxon rank-sum test, and 0.79, resp.. However, the LI-4 site was associated with significant increase in variability compared to its control as denoted by standard deviation and range ( and 0.0005, resp.. At the PC-6 site, no statistical differences in variability were observed. Conclusion. Acupuncture points may be associated with increased variability in electrical potential.

  10. Estimate of electrical potential difference between plasmas with different degrees of ionization

    Energy Technology Data Exchange (ETDEWEB)

    Chang, Chong [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2017-01-12

    The electrical potential difference has been estimated across the mixing region of two plasmas with different degrees of ionization. The estimation has been carried out in two different contexts of a charge neutral mixing region and a charge non-neutral sheath. Ion energy gained due to the potential difference has also been estimated. In both analyses, ion energy gain is proportional to the degree of ionization, and a fairly large ionization appears to be needed for overcoming the potential energy barrier of strongly coupled plasmas.

  11. NON-UNIFORMITY OF THE DISTRIBUTION OF ELECTRICAL TRANSMEMBRANE POTENTIALS IN CARDIAC DEFIBRILLATION

    Directory of Open Access Journals (Sweden)

    O. E. Gudkova

    2015-01-01

    Full Text Available The need for emergency cessation of ventricular fibrillation is one of the problems of modern reanimatology. In spite of searches for novel methods, there is the only effective method — electrical cardiac defibrillation.Objective: to investigate the effect of different forms of pulses on cell membranes in a model experiment and to assess their use for effective cardiac defibrillation.Materials and methods. The Maxwell model was used for theoretical analysis of the spatial distribution of an electric field in the red blood cell membrane. The electric effect on a single cell was calculated using the experimental findings and the equivalent electrical circuit of the myocardial structure during a defibrillation procedure. The cardiomyocyte membrane potential upon exposure to defibrillator discharge was estimated. Exposure of the red blood cell membrane to single, two unipolar and two heteropolar pulses was examined.Results. There is non-additivity of speeds upon double exposure as compared to single one. Single pulse causes a lower effect of electroporation than two double pulses. Hyperpolarization and depolarization processes in the cardiomyocyte membranes occur successively during electrical cardiac defibrillation.Conclusion. Two heteropolar pulses cause an effect of biological membrane electroporation with a greater probability than two unipolar ones.

  12. Theoretical analysis of transmembrane potential of cells exposed to nanosecond pulsed electric field.

    Science.gov (United States)

    Lu, Wei; Wu, Ke; Hu, Xiangjun; Xie, Xiangdong; Ning, Jing; Wang, Changzhen; Zhou, Hongmei; Yang, Guoshan

    2017-02-01

    Intracellular electroporation occurs when the cells are exposed to nanosecond pulsed electric field (nsPEF). It is believed the electroporation (formation and extension of pores on the membrane induced by external electric field) is affected significantly by the transmembrane potential. This paper analyzed transmembrane potential induced by nsPEF in the term of pulse frequency spectrum, aiming to provide a theoretical explanation to intracellular bio-effects. Based on the double-shelled spherical cell model, the frequency dependence of transmembrane potential was obtained by solving Laplace's equation, while the time course of transmembrane potential was obtained by a method combined with discrete Fourier transform and Laplace transform. First-order Debye equation was used to describe the dielectric relaxation of the cell medium. Frequency-domain analysis showed that when the electric field frequency was higher than 10 5 Hz, the transmembrane potential on the organelle membrane (ΔΦ o ) was increasing to exceed the transmembrane potential on the cellular membrane (ΔΦ c ). In the time-domain analysis, transmembrane potentials induced by four nsPEF (short trapezoid, long trapezoid, bipolar and sine shapes) with the same field strength were compared with each other. It showed that ΔΦ o is obviously larger than ΔΦ c if the curve of the normalized frequency spectrum of the pulse is more similar with the curve of normalized ΔΦ o in frequency domain. Pulses with major frequency components higher than 10 8 Hz lead to both small ΔΦ o and ΔΦ c . This may explain why high power pulsed microwave lead to unobvious bio-effects of cells than nsPEF with trapezoid form. Through the pulse frequency spectrum it is clearer to understand the relationship between nsPEF and the transmembrane potential.

  13. Computational Modeling of Single Neuron Extracellular Electric Potentials and Network Local Field Potentials using LFPsim.

    Science.gov (United States)

    Parasuram, Harilal; Nair, Bipin; D'Angelo, Egidio; Hines, Michael; Naldi, Giovanni; Diwakar, Shyam

    2016-01-01

    Local Field Potentials (LFPs) are population signals generated by complex spatiotemporal interaction of current sources and dipoles. Mathematical computations of LFPs allow the study of circuit functions and dysfunctions via simulations. This paper introduces LFPsim, a NEURON-based tool for computing population LFP activity and single neuron extracellular potentials. LFPsim was developed to be used on existing cable compartmental neuron and network models. Point source, line source, and RC based filter approximations can be used to compute extracellular activity. As a demonstration of efficient implementation, we showcase LFPs from mathematical models of electrotonically compact cerebellum granule neurons and morphologically complex neurons of the neocortical column. LFPsim reproduced neocortical LFP at 8, 32, and 56 Hz via current injection, in vitro post-synaptic N2a, N2b waves and in vivo T-C waves in cerebellum granular layer. LFPsim also includes a simulation of multi-electrode array of LFPs in network populations to aid computational inference between biophysical activity in neural networks and corresponding multi-unit activity resulting in extracellular and evoked LFP signals.

  14. Constant electric field simulations of the membrane potential illustrated with simple systems.

    Science.gov (United States)

    Gumbart, James; Khalili-Araghi, Fatemeh; Sotomayor, Marcos; Roux, Benoît

    2012-02-01

    Advances in modern computational methods and technology make it possible to carry out extensive molecular dynamics simulations of complex membrane proteins based on detailed atomic models. The ultimate goal of such detailed simulations is to produce trajectories in which the behavior of the system is as realistic as possible. A critical aspect that requires consideration in the case of biological membrane systems is the existence of a net electric potential difference across the membrane. For meaningful computations, it is important to have well validated methodologies for incorporating the latter in molecular dynamics simulations. A widely used treatment of the membrane potential in molecular dynamics consists of applying an external uniform electric field E perpendicular to the membrane. The field acts on all charged particles throughout the simulated system, and the resulting applied membrane potential V is equal to the applied electric field times the length of the periodic cell in the direction perpendicular to the membrane. A series of test simulations based on simple membrane-slab models are carried out to clarify the consequences of the applied field. These illustrative tests demonstrate that the constant-field method is a simple and valid approach for accounting for the membrane potential in molecular dynamics studies of biomolecular systems. This article is part of a Special Issue entitled: Membrane protein structure and function. Copyright © 2011 Elsevier B.V. All rights reserved.

  15. Alteration in cell surface properties of Burkholderia spp. during surfactant-aided biodegradation of petroleum hydrocarbons

    Energy Technology Data Exchange (ETDEWEB)

    Mohanty, Sagarika; Mukherji, Suparna [Indian Institute of Technology Bombay, Mumbai (India). Centre for Environmental Science and Engineering (CESE)

    2012-04-15

    Chemical surfactants may impact microbial cell surface properties, i.e., cell surface hydrophobicity (CSH) and cell surface charge, and may thus affect the uptake of components from non-aqueous phase liquids (NAPLs). This work explored the impact of Triton X-100, Igepal CA 630, and Tween 80 (at twice the critical micelle concentration, CMC) on the cell surface characteristics of Burkholderia cultures, Burkholderia cepacia (ES1, aliphatic degrader) and Burkholderia multivorans (NG1, aromatic degrader), when grown on a six-component model NAPL. In the presence of Triton X-100, NAPL biodegradation was enhanced from 21% to 60% in B. cepacia and from 18% to 53% in B. multivorans. CSH based on water contact angle (50-52 ) was in the same range for both strains while zeta potential at neutral pH was -38 and -31 mV for B. cepacia and B. multivorans, respectively. In the presence of Triton X-100, their CSH increased to greater than 75 and the zeta potential decreased. This induced a change in the mode of uptake and initiated aliphatic hydrocarbon degradation by B. multivorans and increased the rate of aliphatic hydrocarbon degradation in B. cepacia. Igepal CA 630 and Tween 80 also altered the cell surface properties. For B. cepacia grown in the presence of Triton X-100 at two and five times its CMC, CSH increased significantly in the log growth phase. Growth in the presence of the chemical surfactants also affected the abundance of chemical functional groups on the cell surface. Cell surface changes had maximum impact on NAPL degradation in the presence of emulsifying surfactants, Triton X-100 and Igepal CA630.

  16. The Market for electric vehicles – what do potential users want

    DEFF Research Database (Denmark)

    Christensen, Linda; Kveiborg, Ole; Mabit, Stefan Lindhard

    This paper investigates some of the factors that influence the potential mass introduction of electric vehicles. The main contribution of the paper is an analysis of how recharging influences the demand. We do this by a joint analysis that includes estimation of a model predicting demand for elec......-economic assessments are carried out. The socio-economic assessment shows that the revenue impacts for the government as well as the price of the car and the electricity consumption are still key issues in this aspect.......This paper investigates some of the factors that influence the potential mass introduction of electric vehicles. The main contribution of the paper is an analysis of how recharging influences the demand. We do this by a joint analysis that includes estimation of a model predicting demand...... for electric vehicles based upon price, driving range, acceleration, and accessibility to recharging, an in depth analysis of the drivers’ need for recharging based on their observed driving patterns found in the National Travel Survey and a GSP based recording of driving behaviour of a sample of drivers...

  17. Surface plasmon resonance biomolecular recognition nanosystem: influence of the interfacial electrical potential.

    Science.gov (United States)

    Lopatynskyi, Andrii; Guiver, Michael; Chegel, Volodymyr

    2014-09-01

    It is shown that the response of a surface plasmon resonance nanosystem designed according to Kretschmann geometry on the application of an external electric potential to the gold-electrolyte interface is well described by the proposed mathematical model, which takes into account the geometric surface imperfection and dependence of optical constants of the surface layer of gold film and capacitance of the electrical double layer on applied voltage. This model allows the appropriate correction for results of electrochemical surface plasmon resonance measurements. The dependence of a value of biomolecules adsorption in a surface plasmon resonance nanosystem on applied electric potential is shown for the first time. It is found that a shift of surface plasmon resonance angular position (Δθ(SPR)) and a change of capacitance of electrical double layer on the surface of gold (ΔC(dl)) for the adsorption of proteins under applied voltage are related to the nonlinear dependence Δθ(SPR) = (a + b x ΔC(dl))(-1). This phenomenon can be exploited in biochemical analysis to monitor the interaction of biomolecules, enhance response of biosensors, block unwanted adsorption, etc.

  18. Quasi-polynomial 3D electric and magnetic potentials homogeneous in Euler's sense

    Directory of Open Access Journals (Sweden)

    Alexander S. Berdnikov

    2017-03-01

    Full Text Available Electric and magnetic fields homogeneous in Euler's sense are a useful instrument for designing the systems of charge particle optics. The similarity principle for the charged particle trajectories in these fields was applied by Golikov for the first time to create spectrographic charge particle optical systems in a more systematic and intelligence way when using the fields being homogeneous in Euler's sense. This paper studies the Laplace potentials homogeneous in Euler's sense. The coefficients of the polynomials are functions of the two rest coordinates; they are presented not by the polynomial but ought to be the functions harmonic and homogeneous in Euler's sense. We have solved a finite chain of Poisson equations starting from the highest coefficients. By means of the proposed procedure we obtained new classes of potentials which provided a base for electric and magnetic spectrograph systems.

  19. Estimate of the electric energy generating potential for different sources of biogas in Brazil

    Energy Technology Data Exchange (ETDEWEB)

    Salomon, Karina Ribeiro; Silva Lora, Electo Eduardo [Federal University of Itajuba - UNIFEI, Excellence Group in Thermal Power and Distributed Generation - NEST/IEM, Av BPS 1303, CP 50, Itajuba, MG, CEP 37.500-903 (Brazil)

    2009-09-15

    The increasing interest in the recuperation of the biogas coming from organic residues, associated with its energetic use is a subject that has been widely discussed. Biogas was merely seen as a sub-product obtained from anaerobic decomposition (without oxygen) of organic residue. In the paper is carried out an evaluation of the quantities of organic residues coming out from the sugar and alcohol industry (vinasse), urban solid and liquid wastes (garbage and sewage) and livestock residues (bovine and swine manure) in Brazil. Finally the electricity generation potential of biogas out of the evaluated sources of organic residues in Brazil is estimated. The results of this study indicate that the potential regarding the production of biogas out of the aforementioned organic residues of electricity production using could meet an energy demand of about 1.05 to 1.13 %. Constraints for biogas energy utilization are identified and discussed. (author)

  20. Electrical potentials between stent-grafts made from different metals induce negligible corrosion.

    Science.gov (United States)

    Kazimierczak, A; Podraza, W; Lenart, S; Wiernicki, I; Gutowski, P

    2013-10-01

    Evaluation of the risk of galvanic corrosion in various stent-grafts in current practice, when devices with unmatched alloy compositions are deployed together. Five nitinol (NT) and two steel (SS) stent-grafts produced by different companies were used in different combinations to create 21 samples (NT:NT, n = 10; NT:SS, n = 10; SS:SS, n = 1). Electric potential was measured between the metal couplings after immersion in 0.9% NaCl at a temperature of 37 °C. Subsequently, the same samples were incubated for 24 months in 0.9% NaCl at 37-39 °C under hermetic conditions and examined under a scanning electron microscope in order to search for any evidence of corrosion. Electric potentials between different metals alloys were found (means: NT:SS, 181 μV; NT:NT, 101 μV; SS:SS, 160 μV). The mean electrical potential between stainless steel and nitinol samples was significantly higher than between NT:NT couplings (p corrosion (>10 μm) on a nitinol surface was found (associated with previous mechanical damage) in an NT:SS sample after 24 months of incubation in vitro and no sign of mechanical failure of the wires was found. Direct contact between the stainless steel and the nitinol alloys does indeed create electrical potential but with a minimal risk of galvanic corrosion. No evidence was found for significant galvanic corrosion when two endovascular implants (stent-grafts) made from different metal composition were used in the same procedure. Copyright © 2013 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.

  1. Detection of Fatigue Crack in Basalt FRP Laminate Composite Pipe using Electrical Potential Change Method

    Science.gov (United States)

    Altabey, Wael A.; Noori, Mohammed

    2017-05-01

    Novel modulation electrical potential change (EPC) method for fatigue crack detection in a basalt fibre reinforced polymer (FRP) laminate composite pipe is carried out in this paper. The technique is applied to a laminate pipe with an embedded crack in three layers [0º/90º/0º]s. EPC is applied for evaluating the dielectric properties of basalt FRP pipe by using an electrical capacitance sensor (ECS) to discern damages in the pipe. Twelve electrodes are mounted on the outer surface of the pipe and the changes in the modulation dielectric properties of the piping system are analyzed to detect damages in the pipe. An embedded crack is created by a fatigue internal pressure test. The capacitance values, capacitance change and node potential distribution of ECS electrodes are calculated before and after crack initiates using a finite element method (FEM) by ANSYS and MATLAB, which are combined to simulate sensor characteristics and fatigue behaviour. The crack lengths of the basalt FRP are investigated for various number of cycles to failure for determining crack growth rate. Response surfaces are adopted as a tool for solving inverse problems to estimate crack lengths from the measured electric potential differences of all segments between electrodes to validate the FEM results. The results show that, the good convergence between the FEM and estimated results. Also the results of this study show that the electrical potential difference of the basalt FRP laminate increases during cyclic loading, caused by matrix cracking. The results indicate that the proposed method successfully provides fatigue crack detection for basalt FRP laminate composite pipes.

  2. Comparison of magnetic field and electric potential produced by frog heart muscle

    Science.gov (United States)

    Burstein, Deborah; Cohen, David

    1985-04-01

    A comparison is made here between the magnetic field and electric potential produced by a thin strip of frog heart muscle. An experimental test is made of the theory which states that the wave front of a single fiber (or parallel bundle of fibers as in this strip) can be represented, for both the magnetic field and electric potential, by the same single-current dipole. First, an experimental measurement is made of the ratio of magnetic field/electric potential produced by an actual current dipole in an electrolytic tank. Then the dipole is replaced by the muscle strip and a measurement is again made of the ratio; this is done for three muscle strips at eight different source-to-detector distances ranging from 1 to 5 cm. It is found, in all cases, that the muscle ratios are equal to those of the actual dipole to within the experimental uncertainty of ±10%. Therefore, to this extent the theory is verified for this case of a thin strip of frog heart tissue.

  3. Electrical Retrieval of Living Microorganisms from Cryopreserved Marine Sponges Using a Potential-Controlled Electrode.

    Science.gov (United States)

    Koyama, Sumihiro; Nishi, Shinro; Tokuda, Maki; Uemura, Moeka; Ishikawa, Yoichi; Seya, Takeshi; Chow, Seinen; Ise, Yuji; Hatada, Yuji; Fujiwara, Yoshihiro; Tsubouchi, Taishi

    2015-10-01

    The purpose of this study was to develop a novel electrical retrieval method (ER method) for living sponge-associated microorganisms from marine sponges frozen at -80 °C. A -0.3-V vs. Ag/AgCl constant potential applied for 2 h at 9 °C induced the attachment of the sponge-associated microorganisms to an indium tin oxide/glass (ITO) or a gallium-doped zinc oxide/glass (GZO) working electrode. The electrically attached microorganisms from homogenized Spirastrella insignis tissues had intact cell membranes and showed intracellular dehydrogenase activity. Dead microorganisms were not attracted to the electrode when the homogenized tissues were autoclaved for 15 min at 121 °C before use. The electrically attached microorganisms included cultivable microorganisms retrieved after detachment from the electrode by application of a 9-MHz sine-wave potential. Using the ER method, we obtained 32 phyla and 72 classes of bacteria and 3 archaea of Crenarchaeota thermoprotei, Marine Group I, and Thaumarchaeota incertae sedis from marine sponges S. insignis and Callyspongia confoederata. Employment of the ER method for extraction and purification of the living microorganisms holds potential of single-cell cultivation for genome, transcriptome, proteome, and metabolome analyses of bioactive compounds producing sponge-associated microorganisms.

  4. Potential of Electricity Generation on the Western Coast of Mediterranean Sea in Egypt

    Science.gov (United States)

    Ahmed Shata, A. S.; Abdelaty, S. M.; Hanitsch, R.

    2008-01-01

    A technical and economic assessment has been made of the electricity generation by wind turbines located at three promising potential wind sites: Sidi Barrani, Mersa Matruh and El Dabaa in the extreme northwest of Egypt along the Mediterranean Sea. These contiguous stations along the coast have an annual mean wind speed greater than 5.0 m/s at a height of 10 m. Weibull's parameters and the power law coefficient for all seasons have been estimated and used to describe the distribution and behavior of seasonal winds at these stations. The annual values of wind potential at the heights of 70-100 m above the ground level were obtained by extrapolation of the 10 m data from the results of our previous work using the power law. The three stations have a high wind power density, ranging from 340-425 to 450-555 W/m2 at the heights of 70-100 m, respectively. In this paper, an analysis of the cost per kWh of electricity generated by two different systems has been made: one using a relatively large single 2 MW wind turbine and the other - 25 small wind turbines (80 kW, total 2 MW) arranged in a wind farm. The yearly energy output of each system at each site was determined, and the electricity generation costs in each case were also calculated and compared with those at using diesel oil, natural gas and photovoltaic systems furnished by the Egyptian Electricity Authority. The single 2 MW wind turbine was found to be more efficient than the wind farm. For all the three considered stations the electricity production cost was found to be less than 2 ɛ cent/kWh, which is about half the specific cost of the wind farm.

  5. Evolution of the electric potential of an insulator under charged particle impact

    Science.gov (United States)

    Giglio, E.; Guillous, S.; Cassimi, A.; Zhang, H. Q.; Nagy, G. U. L.; Tőkési, K.

    2017-03-01

    Insulating glass capillaries have been shown to lead to ion transmission without any change in either the ion charge state or in the ion kinetic energy. This surprising process has been attributed to a self-organized distribution of charge patches creating the necessary guiding electric potential on the capillary walls. By the use of our original electrometer, it has been possible to measure and monitor simultaneously and in a nondestructive way the electric potential and the transmitted beam intensity during the charging up by an Ar+ ion beam. We show that glass microcapillaries can reach potentials higher than 500 V, even in the case of singly charged ions, opening the possibility of high transmission rates and providing a renewed sight into ion beam transport by tapered capillaries. The setup, also suitable for the determination of leakage currents governing the capillary potential dynamics, allowed one to evidence that secondary electrons may strongly affect the rise of the capillary potential and consequently avoid Coulomb blocking of the beam transmission across insulating capillaries.

  6. Potential Benefits of an Integrated Electric-Acoustic Sound Processor with Children: A Preliminary Report.

    Science.gov (United States)

    Wolfe, Jace; Neumann, Sara; Schafer, Erin; Marsh, Megan; Wood, Mark; Baker, R Stanley

    2017-02-01

    A number of published studies have demonstrated the benefits of electric-acoustic stimulation (EAS) over conventional electric stimulation for adults with functional low-frequency acoustic hearing and severe-to-profound high-frequency hearing loss. These benefits potentially include better speech recognition in quiet and in noise, better localization, improvements in sound quality, better music appreciation and aptitude, and better pitch recognition. There is, however, a paucity of published reports describing the potential benefits and limitations of EAS for children with functional low-frequency acoustic hearing and severe-to-profound high-frequency hearing loss. The objective of this study was to explore the potential benefits of EAS for children. A repeated measures design was used to evaluate performance differences obtained with EAS stimulation versus acoustic- and electric-only stimulation. Seven users of Cochlear Nucleus Hybrid, Nucleus 24 Freedom, CI512, and CI422 implants were included in the study. Sentence recognition (assayed using the pediatric version of the AzBio sentence recognition test) was evaluated in quiet and at three fixed signal-to-noise ratios (SNR) (0, +5, and +10 dB). Functional hearing performance was also evaluated with the use of questionnaires, including the comparative version of the Speech, Spatial, and Qualities, the Listening Inventory for Education Revised, and the Children's Home Inventory for Listening Difficulties. Speech recognition in noise was typically better with EAS compared to participants' performance with acoustic- and electric-only stimulation, particularly when evaluated at the less favorable SNR. Additionally, in real-world situations, children generally preferred to use EAS compared to electric-only stimulation. Also, the participants' classroom teachers observed better hearing performance in the classroom with the use of EAS. Use of EAS provided better speech recognition in quiet and in noise when compared to

  7. Potential reuse of small household waste electrical and electronic equipment: Methodology and case study.

    Science.gov (United States)

    Bovea, María D; Ibáñez-Forés, Valeria; Pérez-Belis, Victoria; Quemades-Beltrán, Pilar

    2016-07-01

    This study proposes a general methodology for assessing and estimating the potential reuse of small waste electrical and electronic equipment (sWEEE), focusing on devices classified as domestic appliances. Specific tests for visual inspection, function and safety have been defined for ten different types of household appliances (vacuum cleaner, iron, microwave, toaster, sandwich maker, hand blender, juicer, boiler, heater and hair dryer). After applying the tests, reuse protocols have been defined in the form of easy-to-apply checklists for each of the ten types of appliance evaluated. This methodology could be useful for reuse enterprises, since there is a lack of specific protocols, adapted to each type of appliance, to test its potential of reuse. After applying the methodology, electrical and electronic appliances (used or waste) can be segregated into three categories: the appliance works properly and can be classified as direct reuse (items can be used by a second consumer without prior repair operations), the appliance requires a later evaluation of its potential refurbishment and repair (restoration of products to working order, although with possible loss of quality) or the appliance needs to be finally discarded from the reuse process and goes directly to a recycling process. Results after applying the methodology to a sample of 87.7kg (96 units) show that 30.2% of the appliances have no potential for reuse and should be diverted for recycling, while 67.7% require a subsequent evaluation of their potential refurbishment and repair, and only 2.1% of them could be directly reused with minor cleaning operations. This study represents a first approach to the "preparation for reuse" strategy that the European Directive related to Waste Electrical and Electronic Equipment encourages to be applied. However, more research needs to be done as an extension of this study, mainly related to the identification of the feasibility of repair or refurbishment operations

  8. The potential for electricity efficiency improvements in the US Residential Sector

    Energy Technology Data Exchange (ETDEWEB)

    Koomey, J.G.; Atkinson, C.; Meier, A.; McMahon, J.E.; Boghosian, S.; Atkinson, B.; Turiel, I.; Levine, M.D.; Nordman, B.; Chan, P.

    1991-07-01

    This study represents the most elaborate assessment to date of US residential sector electricity improvements. Previous analyses have estimated the conservation potential for other countries, states, or individual utility service territories. As concern over greenhouse gas emissions has increased, interest has grown in estimates of conservation potential for the US residential sector as a whole. The earliest detailed estimate of US conservation potential is now out of date, while more recent estimates are less detailed than is desirable for engineering-economic estimates of the costs of reducing carbon emissions. In this paper, we first describe the methodology for creating supply curves of conserved energy, and then illustrate the subtleties of assessing the technical conservation potential. Next we present the data and forecasts used in this assessment, including costs, baseline thermal characteristics, energy use, and energy savings. Finally, we present the main results and conclusions from the analysis, and discuss future work. 102 refs., 7 figs., 16 tabs.

  9. Detection and Elimination of a Potential Fire in Engine and Battery Compartments of Hybrid Electric Vehicles

    Directory of Open Access Journals (Sweden)

    Macam S. Dattathreya

    2012-01-01

    Full Text Available This paper presents a novel fuzzy deterministic noncontroller type (FDNCT system and an FDNCT inference algorithm (FIA. The FDNCT uses fuzzy inputs and produces a deterministic non-fuzzy output. The FDNCT is an extension and alternative for the existing fuzzy singleton inference algorithm. The research described in this paper applies FDNCT to build an architecture for an intelligent system to detect and to eliminate potential fires in the engine and battery compartments of a hybrid electric vehicle. The fuzzy inputs consist of sensor data from the engine and battery compartments, namely, temperature, moisture, and voltage and current of the battery. The system synthesizes the data and detects potential fires, takes actions for eliminating the hazard, and notifies the passengers about the potential fire using an audible alarm. This paper also presents the computer simulation results of the comparison between the FIA and singleton inference algorithms for detecting potential fires and determining the actions for eliminating them.

  10. Assessment of the Potential of Biomass Gasification for Electricity Generation in Bangladesh

    Directory of Open Access Journals (Sweden)

    Barun Kumar Das

    2014-01-01

    Full Text Available Bangladesh is an agriculture based country where more than 65 percent of the people live in rural areas and over 70% of total primary energy consumption is covered by biomass, mainly agricultural waste and wood. Only about 6% of the entire population has access to natural gas, primarily in urban areas. Electricity production in Bangladesh largely depends on fossil fuel whose reserve is now under threat and the government is now focusing on the alternating sources to harness electricity to meet the continuous increasing demand. To reduce the dependency on fossil fuels, biomass to electricity could play a vital role in this regard. This paper explores the biomass based power generation potential of Bangladesh through gasification technology—an efficient thermochemical process for distributed power generation. It has been estimated that the total power generation from the agricultural residue is about 1178 MWe. Among them, the generation potential from rice husk, and bagasses is 1010 MWe, and 50 MWe, respectively. On the other hand, wheat straw, jute stalks, maize residues, lentil straw, and coconut shell are also the promising biomass resources for power generation which counted around 118 MWe. The forest residue and municipal solid waste could also contribute to the total power generation 250 MWe and 100 MWe, respectively.

  11. HPMA copolymer-aminohexylgeldanamycin conjugates targeting cell surface expressed GRP78 in prostate cancer.

    Science.gov (United States)

    Larson, Nate; Ray, Abhijit; Malugin, Alexander; Pike, Daniel B; Ghandehari, Hamidreza

    2010-12-01

    This study focused on the synthesis and in vitro characterization of N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer conjugates for the delivery of geldanamycin to prostate cancer tumors. Conjugates were modified to incorporate WIFPWIQL peptide, which binds to cell-surface-expressed Glucose-regulated protein 78. HPMA copolymers containing aminohexylgeldanamycin with and without WIFPWIQL peptide were synthesized and characterized, and stability in pH 7.4 and pH 5.0 buffers, complete cell culture medium, and fetal bovine serum was evaluated. The comparative cell surface expression of GRP78 in DU145 and PC3 cell lines was assessed and competitive binding to cell surface expressed GRP78 evaluated. The ability of the conjugates to inhibit cell growth was also evaluated in vitro. HPMA copolymer-aminohexylgeldanamycin conjugates were stable with maximal release observed in fetal bovine serum at 37°C of approximately 10% in 72 h. HPMA copolymers bearing WIFPWIQL peptide bound to cell surface expressed GRP78 with affinities comparable to free WIFPWIQL peptide and demonstrated increased cytotoxicity as compared to untargeted conjugates. HPMA copolymer aminohexylgeldanamycin conjugates bearing WIFPWIQL peptide have the ability to bind to cell-surface-expressed GRP78 and inhibit the growth of human prostate cancer cells, suggesting that the conjugates have the potential to target solid prostate cancer tumors.

  12. Electricity production potential and social benefits from rice husk, a case study in Pakistan

    Directory of Open Access Journals (Sweden)

    Obaidullah Mohiuddin

    2016-12-01

    Full Text Available Pakistan has been experiencing energy crisis owing to its sole dependence on fossil fuels. Reduction in local fossil fuel reserves has led to an increase in their prices, thereby increasing the cost of electricity. Since the tariff remains the same, Pakistan is over-burdened with circular debts and observes a daily power shortfall of about 12–14 h. Being an Agra-economic country, many major and minor crops are produced and exported in large quantities. This results in a bulk of the agricultural waste which are not utilized. The waste can be utilized to meet the country’s energy demand while mitigating climate change and its impact. The study examines the electricity production potential and social benefits of rice husk in Pakistan. It is estimated in this study that if 70% of rice husk residues are utilized, there will be annual electricity production of 1,328 GWh and the cost of per unit electricity by rice husk is found at 47.36 cents/kWh as compared to 55.22 cents/kWh of electricity generated by coal. Importantly, the study will increase the awareness of the benefits of utilizing agricultural waste for useful products such as silica, with several social and environmental benefits such as a reduction of 36,042 tCO2e/yr of methane, reducing carbon dioxide emissions, improving the air quality, and providing 4.5 k new jobs. The paper concludes with the policy recommendations based on this study.

  13. Electric energy saving potential by substitution of domestic refrigerators in Mexico

    Energy Technology Data Exchange (ETDEWEB)

    Arroyo-Cabanas, F.G. [Posgrado en Ingenieria, Universidad Nacional Autonoma de Mexico, A. P. 20-364, Mexico, Distrito Federal, C.P. 05500 (Mexico); Programa de Energia, Universidad Autonoma de la Ciudad de Mexico, San Lorenzo 290, Col. Del Valle, Del. Benito Juarez, Mexico, Distrito Federal, C.P. 03100 (Mexico); Aguillon-Martinez, J.E. [Instituto de Ingenieria, Universidad Nacional Autonoma de Mexico, A. P. 20-364, Mexico, Distrito Federal, C.P. 05500 (Mexico); Ambriz-Garcia, J.J. [Ingenieria en Recursos Energeticos, Universidad Autonoma Metropolitana, Av. San Rafael Atlixco 186, Col. Vicentina, C.P. 09340, Iztapalapa, Mexico, Distrito Federal (Mexico); Canizal, G. [Programa de Energia, Universidad Autonoma de la Ciudad de Mexico, San Lorenzo 290, Col. Del Valle, Del. Benito Juarez, Mexico, Distrito Federal, C.P. 03100 (Mexico)

    2009-11-15

    We evaluate the potential for electric power saving in Mexico that would follow the substitution of old, low efficiency domestic refrigerators with modern, high efficiency ones. Our results indicate that a total substitution of domestic refrigerators would save 4.7 TWh/year, which represents 33% of the annual total consumption of 14.1 TWh. Assuming an average daily use of 14 h for domestic refrigeration, 900 MW of electricity would be saved. An annual substitution of 20% over 5 years would save approximately 1 TWh a year and almost 180 MW would be released. It is recommended that this program of replacement should be supported by the Federal and State governments, beginning with the States (which have more direct influence), and with the oldest, least efficient refrigerators along the lines followed in programs that introduced fluorescent compact lamps. (author)

  14. Profiling the Built-in Electrical Potential in III-V Multijunction Solar Cells: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Jiang, C.-S.; Friedman, D. J.; Moutinho, H. R.; Al-Jassim, M. M.

    2006-05-01

    We report on a direct measurement of the electrical potential on cross-sections of GaInP2/GaAs multiple-junction solar cells by using an ultrahigh-vacuum scanning Kelvin probe microscope (UHV-SKPM). The UHV-SKPM allows us to measure the potential without air molecules being adsorbed on the cross-sectional surface. Moreover, it uses a GaAs laser with photon energy of 1.4 eV for the atomic force microscope (AFM) operation. This eliminated the light-absorption-induced bottom-junction flattening and top-junction enhancement, which happened in our previous potential measurement using a 1.85-eV laser for the AFM operation. Three potentials were measured at the top, tunneling, and bottom junctions. Values of the potentials are smaller than the potentials in the bulk. This indicates that the Fermi level on the UHV-cleaved (110) surface was pinned, presumably due to defects upon cleaving. We also observed higher potentials at atomic steps than on the terraces for both GaInP2 epitaxial layer and GaAs substrate. Combining scanning tunneling microscopy (STM) and SKPM measurements, we found that the potential height at steps of the GaAs substrate depends on the step direction, which is probably a direct result of unbalanced cations and anions at the steps.

  15. Electrical Potentials Observed During Frictional Stick-Slip - A Semiconductor Mechanism

    Science.gov (United States)

    Leeman, J.; Scuderi, M.; Marone, C.; Saffer, D. M.

    2013-12-01

    Electromagnetic phenomena are commonly reported during and after large earthquakes. Various lines of evidence including charring of plant roots, magnetic remnant signatures in pseudotachylite, and visible earthquake lights indicate a strong electrical potential separation during co-seismic rupture. Suggested explanations have included triboelectricity, piezoelectricity, and streaming potentials. The 'semiconductor effect', or migration of electron holes, has been proposed as an alternative explanation and studied extensively in solids. We present evidence of a similar migration effect in a granular material that exhibits repeated frictional stick-slip events under a variety of conditions. Soda-lime glass beads were sheared in a double-direct shear configuration in a biaxial loading frame. Glass beads exhibit consistent, repetitive stick-slip and rate/state friction effects that are similar to rock. Layers of 5 mm thickness were sheared under a constant normal load of 4MPa, at load point velocities of 1, 30, and 100 μm/s. This was done for mono-disperse particle size distributions of 100-150 μm and 420-500 μm. Tests were conducted at room humidity, at 100% humidity, and under submerged conditions. During shearing, the electrical potential of the surface was monitored relative to the system ground with a non-contact electrostatic volt meter (ESVM) manufactured by Trek Incorporated. During stick-slip events, we observe electrical potential anomalies that appear to be related to failure of force chains supporting the shear load. Two distinct types of behavior are delineated by the attainment of steady state frictional sliding. In the pre-steady state phase, as shear stress is increasing, layers are observed to charge during stick-slip and the potential of the entire system rises. When shear stress rises to the level of steady state frictional sliding, the system begins to discharge, with superimposed anomalies characterized by potential drops of several volts that

  16. Lysyl oxidase drives tumour progression by trapping EGF receptors at the cell surface.

    Science.gov (United States)

    Tang, HaoRan; Leung, Leo; Saturno, Grazia; Viros, Amaya; Smith, Duncan; Di Leva, Gianpiero; Morrison, Eamonn; Niculescu-Duvaz, Dan; Lopes, Filipa; Johnson, Louise; Dhomen, Nathalie; Springer, Caroline; Marais, Richard

    2017-04-18

    Lysyl oxidase (LOX) remodels the tumour microenvironment by cross-linking the extracellular matrix. LOX overexpression is associated with poor cancer outcomes. Here, we find that LOX regulates the epidermal growth factor receptor (EGFR) to drive tumour progression. We show that LOX regulates EGFR by suppressing TGFβ1 signalling through the secreted protease HTRA1. This increases the expression of Matrilin2 (MATN2), an EGF-like domain-containing protein that traps EGFR at the cell surface to facilitate its activation by EGF. We describe a pharmacological inhibitor of LOX, CCT365623, which disrupts EGFR cell surface retention and delays the growth of primary and metastatic tumour cells in vivo. Thus, we show that LOX regulates EGFR cell surface retention to drive tumour progression, and we validate the therapeutic potential of inhibiting this pathway with the small molecule inhibitor CCT365623.

  17. 60 Hz electric field changes the membrane potential during burst phase in pancreatic β-cells: in silico analysis.

    Science.gov (United States)

    Neves, Gesilda F; Silva, José R F; Moraes, Renato B; Fernandes, Thiago S; Tenorio, Bruno M; Nogueira, Romildo A

    2014-06-01

    The production, distribution and use of electricity can generate low frequency electric and magnetic fields (50-60 Hz). Considering that some studies showed adverse effects on pancreatic β-cells exposed to these fields; the present study aimed to analyze the effects of 60 Hz electric fields on membrane potential during the silent and burst phases in pancreatic β-cells using a mathematical model. Sinusoidal 60 Hz electric fields with amplitude ranging from 0.5 to 4 mV were applied on pancreatic β-cells model. The sinusoidal electric field changed burst duration, inter-burst intervals (silent phase) and spike sizes. The parameters above presented dose-dependent response with the voltage amplitude applied. In conclusion, theoretical analyses showed that a 60 Hz electric field with low amplitudes changes the membrane potential in pancreatic β-cells.

  18. Novel eukaryotic enzymes modifying cell-surface biopolymers

    Directory of Open Access Journals (Sweden)

    Aravind L

    2010-01-01

    Full Text Available Abstract Background Eukaryotic extracellular matrices such as proteoglycans, sclerotinized structures, mucus, external tests, capsules, cell walls and waxes contain highly modified proteins, glycans and other composite biopolymers. Using comparative genomics and sequence profile analysis we identify several novel enzymes that could be potentially involved in the modification of cell-surface glycans or glycoproteins. Results Using sequence analysis and conservation we define the acyltransferase domain prototyped by the fungal Cas1p proteins, identify its active site residues and unify them to the superfamily of classical 10TM acyltransferases (e.g. oatA. We also identify a novel family of esterases (prototyped by the previously uncharacterized N-terminal domain of Cas1p that have a similar fold as the SGNH/GDSL esterases but differ from them in their conservation pattern. Conclusions We posit that the combined action of the acyltransferase and esterase domain plays an important role in controlling the acylation levels of glycans and thereby regulates their physico-chemical properties such as hygroscopicity, resistance to enzymatic hydrolysis and physical strength. We present evidence that the action of these novel enzymes on glycans might play an important role in host-pathogen interaction of plants, fungi and metazoans. We present evidence that in plants (e.g. PMR5 and ESK1 the regulation of carbohydrate acylation by these acylesterases might also play an important role in regulation of transpiration and stress resistance. We also identify a subfamily of these esterases in metazoans (e.g. C7orf58, which are fused to an ATP-grasp amino acid ligase domain that is predicted to catalyze, in certain animals, modification of cell surface polymers by amino acid or peptides. Reviewers This article was reviewed by Gaspar Jekely and Frank Eisenhaber

  19. Engineered cell surfaces: fertile ground for molecular landscaping.

    Science.gov (United States)

    Mahal, L K; Bertozzi, C R

    1997-06-01

    The cell surface contains a wealth of information that determines how cells interact with their environment. Methods for directing the cell surface expression of novel protein-based and oligosaccharide-based epitopes are stimulating new directions in biotechnology and biomedical research.

  20. IMPLICATIONS OF MICROBIAL ADHESION TO HYDROCARBONS FOR EVALUATING CELL-SURFACE HYDROPHOBICITY .2. ADHESION MECHANISMS

    NARCIS (Netherlands)

    VANDERMEI, HC; VANDEBELTGRITTER, B; BUSSCHER, HJ

    1995-01-01

    Microbial adhesion to hydrocarbons (MATH) is generally considered to be a measure of the organisms cell surface hydrophobicity. Recent observations that the zeta potentials of hydrocarbons can be highly negative in the various solutions commonly used in MATH, have suggested that MATH may measure a

  1. Cell surface engineering of Bacillus subtilis improves production yields of heterologously expressed α-amylases

    NARCIS (Netherlands)

    Cao, Haojie; van Heel, Auke J; Ahmed, Hifza; Mols, Maarten; Kuipers, Oscar P

    2017-01-01

    BACKGROUND: Bacillus subtilis is widely used as a cell factory for numerous heterologous proteins of commercial value and medical interest. To explore the possibility of further enhancing the secretion potential of this model bacterium, a library of engineered strains with modified cell surface

  2. The Cell Surface Structure of Tumor Endothelial Marker 8 (TEM8) is Regulated by the Actin Cytoskeleton

    OpenAIRE

    Yang, Mi Young; Chaudhary, Amit; Seaman, Steven; Dunty, Jill; Stevens, Janine; Elzarrad, Mohammed K.; Frankel, Arthur E.; St. Croix, Brad

    2010-01-01

    Tumor Endothelial Marker 8 (TEM8) is an integrin-like cell surface protein upregulated on tumor blood vessels and a potential vascular target for cancer therapy. Here, we found that the ability of an anti-TEM8 antibody, clone SB5, to recognize the extracellular domain of TEM8 on the cell surface depends on other host-cell factors. By taking advantage of SB5’s ability to distinguish different forms of cell-surface TEM8, we identified alpha-smooth muscle actin and transgelin, an actin binding p...

  3. Postfatigue potentiation of the paralyzed soleus muscle: evidence for adaptation with long-term electrical stimulation training.

    Science.gov (United States)

    Shields, Richard K; Dudley-Javoroski, Shauna; Littmann, Andrew E

    2006-08-01

    Understanding the torque output behavior of paralyzed muscle has important implications for the use of functional neuromuscular electrical stimulation systems. Postfatigue potentiation is an augmentation of peak muscle torque during repetitive activation after a fatigue protocol. The purposes of this study were 1) to quantify postfatigue potentiation in the acutely and chronically paralyzed soleus and 2) to determine the effect of long-term soleus electrical stimulation training on the potentiation characteristics of recently paralyzed soleus muscle. Five subjects with chronic paralysis (>2 yr) demonstrated significant postfatigue potentiation during a repetitive soleus activation protocol that induced low-frequency fatigue. Ten subjects with acute paralysis (stimulation. Seven of these acute subjects completed 2 yr of home-based isometric soleus electrical stimulation training of one limb (compliance = 83%; 8,300 contractions/wk). With the early implementation of electrically stimulated training, potentiation characteristics of trained soleus muscles were preserved as in the acute postinjury state. In contrast, untrained limbs showed marked postfatigue potentiation at 2 yr after spinal cord injury (SCI). A single acute SCI subject who was followed longitudinally developed potentiation characteristics very similar to the untrained limbs of the training subjects. The results of the present investigation support that postfatigue potentiation is a characteristic of fast-fatigable muscle and can be prevented by timely neuromuscular electrical stimulation training. Potentiation is an important consideration in the design of functional electrical stimulation control systems for people with SCI.

  4. Asymptotically de Sitter and anti-de Sitter black holes with confining electric potential

    Science.gov (United States)

    Guendelman, Eduardo; Kaganovich, Alexander; Nissimov, Emil; Pacheva, Svetlana

    2011-10-01

    We study gravity interacting with a special kind of QCD-inspired nonlinear gauge field system which earlier was shown to yield confinement-type effective potential (the "Cornell potential") between charged fermions ("quarks") in flat space-time. We find new static spherically symmetric solutions generalizing the usual Reissner-Nordström-de Sitter and Reissner-Nordström-anti-de Sitter black holes with the following additional properties: (i) appearance of a constant radial electric field (in addition to the Coulomb one); (ii) novel mechanism of dynamical generation of cosmological constant through the non-Maxwell gauge field dynamics; (iii) appearance of confining-type effective potential in charged test particle dynamics in the above black hole backgrounds.

  5. [Effect of stimulating pulse width on the threshold of electrically evoked compound action potential].

    Science.gov (United States)

    Yu, Zhongde; Xiao, Ling; Li, Ping; Meng, Li; Zi, Rui; Fei, Xingbo

    2014-12-01

    This paper discusses the relationship between stimulating pulse width and the threshold of electrically evoked compound action potential (ECAP). Firstly, the rheobase and chronaxy from strength-duration curve of nerve fiber was computed using the shepherd's experiment results. Secondly, based on the relationship between ECAP and the action potential of nerve fiber, a mathematical expression to describe the relationship between stimulating pulse width and ECAP threshold was proposed. Thirdly, the parameters were obtained and the feasibility was proved to the expression with the results of experiment using guinea pigs. Research result showed that with ECAP compared to the action potential of nerve fiber, their threshold function relationship with stimulating pulse width was similar, and rheobase from the former was an order smaller in the magnitude than the latter, but the chronaxy was close to each other. These findings may provide meaningful guidance to clinical ECAP measurement and studying speech processing strategies of cochlear implant.

  6. Potentiation and Electrical Stimulus Frequency During Self-Paced Exercise and Recovery

    Directory of Open Access Journals (Sweden)

    Froyd Christian

    2014-10-01

    Full Text Available The aim of this study was to investigate the effect of potentiation on stimulation-induced muscle function during and after an intense bout of self-paced dynamic exercise. Ten active subjects performed a time trial involving repetitive concentric extension-flexion of the right knee using a Biodex dynamometer. Electrical stimulation before and after a 5 s maximal isometric voluntary contraction was performed before the start of the time trial and immediately (< 5 s after each 20% of the time trial as well as 1, 2, 4 and 8 min after time trial termination. Potentiation was observed before the time trial and as early as 1-2 min after the time trial, but no potentiation was detected during or immediately after the time trial for neither single or paired stimuli. At termination of the time trial, “potentiated” peak torque was significantly more reduced than “unpotentiated" peak torque for single stimulus (-65 ± 10% and -42 ± 18%, respectively and paired stimuli at 100 Hz (-51 ± 10% and -33 ± 15%, respectively. Faster recovery for “potentiated” compared to “unpotentiated” peak torque indicate that potentiate peak torque measurements or delay the post-exercise measurements more than a few seconds, will underestimate peripheral fatigue. In conclusion, the potentiation after maximal contraction disappears during intense exercise. Whether the muscle is already potentiated during intense contraction or fatiguing mechanisms inhibits potentiation remains to be clarified

  7. Electric double layer and electrokinetic potential of pectic macromolecules in sugar beet

    Directory of Open Access Journals (Sweden)

    Kuljanin Tatjana A.

    2008-01-01

    Full Text Available Electrokinetic potential is an important property of colloidal particles and, regarding the fact that it is a well defined and easily measurable property, it is considered to be a permanent characteristic of a particular colloidal system. In fact, it is a measure of electrokinetic charge that surrounds the colloidal particle in a solution and is in direct proportion with the mobility of particles in an electric field. Gouy-Chapman-Stern-Graham's model of electric double layer was adopted and it was proven experimentally that the addition of Cu++ ions to sugar beet pectin caused a reduction in the negative electrokinetic potential proportional to the increase of Cu++ concentration. Higher Cu++ concentrations increased the proportion of cation specific adsorption (Cu++ and H+ with regard to electrostatic Coulombic forces. Consequently, there is a shift in the shear plane between the fixed and diffuse layers directed towards the diffuse layer, i.e. towards its compression and decrease in the electrokinetic potential or even charge inversion of pectin macromolecules.

  8. Evaluation of the constant potential method in simulating electric double-layer capacitors

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Zhenxing; Laird, Brian B., E-mail: blaird@ku.edu [Department of Chemistry, University of Kansas, Lawrence, Kansas 66045 (United States); Yang, Yang; Olmsted, David L.; Asta, Mark [Department of Materials Science and Engineering, University of California, Berkeley, California 94720 (United States)

    2014-11-14

    A major challenge in the molecular simulation of electric double layer capacitors (EDLCs) is the choice of an appropriate model for the electrode. Typically, in such simulations the electrode surface is modeled using a uniform fixed charge on each of the electrode atoms, which ignores the electrode response to local charge fluctuations in the electrolyte solution. In this work, we evaluate and compare this Fixed Charge Method (FCM) with the more realistic Constant Potential Method (CPM), [S. K. Reed et al., J. Chem. Phys. 126, 084704 (2007)], in which the electrode charges fluctuate in order to maintain constant electric potential in each electrode. For this comparison, we utilize a simplified LiClO{sub 4}-acetonitrile/graphite EDLC. At low potential difference (ΔΨ ⩽ 2 V), the two methods yield essentially identical results for ion and solvent density profiles; however, significant differences appear at higher ΔΨ. At ΔΨ ⩾ 4 V, the CPM ion density profiles show significant enhancement (over FCM) of “inner-sphere adsorbed” Li{sup +} ions very close to the electrode surface. The ability of the CPM electrode to respond to local charge fluctuations in the electrolyte is seen to significantly lower the energy (and barrier) for the approach of Li{sup +} ions to the electrode surface.

  9. Measurement of the electric potential at the surface of nonuniformly charged polypropylene nonwoven media

    Science.gov (United States)

    Fatihou, Ali; Zouzou, Noureddine; Iuga, Gheorghe; Dascalescu, Lucian

    2015-10-01

    The aim of this paper is to establish the conditions in which the vibrating capacitive probe of an electrostatic voltmeter could be employed for mapping the electric potential at the surface of non-uniformly charged insulating bodies. A first set of experiments are performed on polypropylene non-woven media (thickness: 0.4 mm; fiber diameter: 20 μm) in ambient air. In a second set of experiments the non-uniformity of charge is simulated using five copper strips (width: 2 mm or 3 mm; distance between strips: 2 mm). All the strips are connected to a high-voltage supply (Vs = 1000 V). The sample carrier is attached to a computer-controlled positioning system that transfers it under the capacitive probe (TREK, model 3451) of an electrostatic voltmeter (TREK, model 1341B). The measurements are performed at various relative speeds Vb between the sample and the probe, and for various sample rates Fe. A first set of experiments point out that the electric potential displayed by the electrostatic voltmeter depends on the spacing h between the sample and the probe. The diameter D of the spot “seen” by the probe is approximately D ≈ 8h/3. From the second set of experiments performed with the test plate, it can be concluded that the surface potential can be measured with the media in motion, but the accuracy is limited by the spatial resolution defined by k = Vb/Fe.

  10. Potential Impact of the National Plan for Future Electric Power Supply on Air Quality in Korea

    Science.gov (United States)

    Shim, C.; Hong, J.

    2014-12-01

    Korean Ministry of Trade, Industry and Energy (MOTIE) announced the national plan for Korea's future electric power supply (2013 - 2027) in 2013. According to the plan, the national demand for electricity will be increased by 60% compared to that of 2010 and primary energy sources for electric generation will still lean on the fossil fuels such as petroleum, LNG, and coal, which would be a potential threat to air quality of Korea. This study focused on two subjects: (1) How the spatial distribution of the primary air pollutant's emissions (i.e., NOx, SOx, CO, PM) will be changed and (2) How the primary emission changes will influence on the national ambient air quality including ozone in 2027. We used GEOS-Chem model simulation with modification of Korean emissions inventory (Clean Air Policy Support System (CAPSS)) to simulate the current and future air quality in Korea. The national total emissions of CO, NOx, SOx, PM in year 2027 will be increased by 3%, 8%, 13%, 2%, respectively compared to 2010 and there are additional concern that the future location of the power plants will be closer to the Seoul Metropolitan Area (SMA), where there are approximately 20 million population vulnerable to the potentially worsened air quality. While there are slight increase of concentration of CO, NOx, SOx, and PM in 2027, the O3 concentration is expected to be similar to the level of 2010. Those results may imply the characteristics of air pollution in East Asia such as potentially severe O3 titration and poorer O3/CO or O3/NOx ratio. Furthermore, we will discuss on the impact of transboundary pollution transport from China in the future, which is one of the large factors to control the air quality of Korea.

  11. Determination of dynamic pressure on infinite piezoelectric hollow cylinder from electric potential difference measurement

    Energy Technology Data Exchange (ETDEWEB)

    Wang, H.M. [Department of Mechanics, School of Aeronautics and Astronautics, Zhejiang University, Hangzhou 310027 (China)], E-mail: wanghuiming@zju.edu.cn; Ding, H.J. [Department of Civil Engineering, College of Civil Engineering and Architecture, Zhejiang University, Hangzhou 310027 (China)

    2009-06-15

    An analytical method is developed for evaluating the dynamic pressure acted at the surface of infinite piezoelectric hollow cylinder by measuring the electric potential difference between the internal and external surfaces. By virtue of the separation of variables method and the orthogonal expansion technique, the inverse boundary problem is transformed to a second kind Volterra integral equation about the unknown dynamic pressure. The interpolation method is employed to solve the integral equation and the dynamic pressure is determined. The present method is suitable for the hollow cylinder with arbitrary thickness subjected to arbitrary dynamic pressure. Numerical experiments are also presented.

  12. The potential of agro-industrial residues for production of biogas and electricity in Tanzania

    Energy Technology Data Exchange (ETDEWEB)

    Kivaisi, A.K.; Rubindamayugi, M.S.T. [Applied Microbiology Unit, Dar es salaam (Tanzania, United Republic of). Botany Dept.

    1996-09-01

    This paper gives an overview of the energy demands in Tanzania, and highlights the current serious shortages of electricity. Government strategy to alleviate the problem include exploitation of the country`s big natural gas reserves for power generation, and utilization of the renewable energies such as solar, wind and biogas. Important agro-industrial residues with a large potential for anaerobic conversion into biogas and electricity are identified, and their production and locations are described. Tanzania generates a total of 468,100 tonnes organic matter from coffee, sisal, sugar and cereal residues annually. Laboratory scale determinations of methane yields from the residues gave 400m{sup 3} CH{sub 4}/ton VS of sisal pulp; 400 m{sup 3} CH{sub 4}/ton VS of sisal production wastewater; 650 m{sup 3} CH{sub 4}/ton VS of Robusta coffee solid waste; 730 m{sup 3} CH{sub 4}/tone of Arabica coffee solid waste; 230 m{sup 3} CH{sub 4}/ton VS of sugar filter mat and 450 m{sup 3} CH{sub 4}/ton VS maize bran. Based on these results the estimated total annual potential electricity production from these residues is 1,135 million kWh. The total oil substitution from these residues has been estimated at 0.32 million tonnes crude diesel oil per annum equivalent to 2% of the total energy consumption in Tanzania. Case studies of the coffee and sisal processing factories indicate that exploitation of the residues for the production of electricity on site of these factories is feasible. Utilization of agro-industrial residues for biogas production has a big potential for reduction of environmental pollution. The potential substitution of fossil fuel with biogas represents an annual reduction in the net CO{sub 2} emission to the atmosphere of approximately 1.05 million tonnes. By treating the residues in controlled anaerobic systems it is possible to reduce the methane emission by about 189 million m{sup 3}, and at the same time reduce contamination of surface and ground waters.

  13. Experimental investigation into the transmembrane electrical potential of the forward osmosis membrane process in electrolyte solutions.

    Science.gov (United States)

    Bian, Lixia; Fang, Yanyan; Wang, Xiaolin

    2014-06-19

    The transmembrane electrical potential (TMEP) in a forward osmosis membrane process with a single electrolyte solution as the draw and feed solutions was investigated by experiments. The effects of membrane orientation, the electrolyte species (KCl, NaCl, MgCl2, and CaCl2), concentration and concentration ratio of solutions at both sides of membrane on water flux and TMEP were investigated. The results showed that the TMEPs at different membrane orientation cannot completely coincide, which confirmed the effect of membrane asymmetry. The ion diffusion coefficients significantly affected the TMEP across the membrane, with different patterns for different electrolytes and concentrations.

  14. Experimental Investigation into the Transmembrane Electrical Potential of the Forward Osmosis Membrane Process in Electrolyte Solutions

    Directory of Open Access Journals (Sweden)

    Lixia Bian

    2014-06-01

    Full Text Available The transmembrane electrical potential (TMEP in a forward osmosis membrane process with a single electrolyte solution as the draw and feed solutions was investigated by experiments. The effects of membrane orientation, the electrolyte species (KCl, NaCl, MgCl2, and CaCl2, concentration and concentration ratio of solutions at both sides of membrane on water flux and TMEP were investigated. The results showed that the TMEPs at different membrane orientation cannot completely coincide, which confirmed the effect of membrane asymmetry. The ion diffusion coefficients significantly affected the TMEP across the membrane, with different patterns for different electrolytes and concentrations.

  15. Energy Savings Potential and Opportunities for High-Efficiency Electric Motors in Residential and Commercial Equipment

    Energy Technology Data Exchange (ETDEWEB)

    Goetzler, William [Navigant Consulting, Inc., Burlington, MA (United States); Sutherland, Timothy [Navigant Consulting, Inc., Burlington, MA (United States); Reis, Callie [Navigant Consulting, Inc., Burlington, MA (United States)

    2013-12-04

    This report describes the current state of motor technology and estimates opportunities for energy savings through application of more advanced technologies in a variety of residential and commercial end uses. The objectives of this report were to characterize the state and type of motor technologies used in residential and commercial appliances and equipment and to identify opportunities to reduce the energy consumption of electric motor-driven systems in the residential and commercial sectors through the use of advanced motor technologies. After analyzing the technical savings potential offered by motor upgrades and variable speed technologies, recommended actions are presented.

  16. Potential Analysis of Regulating Power from Electric Vehicle (EV) Integration in Denmark

    DEFF Research Database (Denmark)

    Wu, Qiuwei; Nielsen, Arne Hejde; Østergaard, Jacob

    2011-01-01

    The potential analysis of having electric vehicles (EVs) provide regulating power has been implemented. The possible regulating power capacity from EVs and the economic return for EV users by providing regulating power are obtained. A spot price based charging schedule scenario has been used to do...... the day-ahead charging schedule for all EVs based on the predicted driving pattern. On top of the obtained charging schedule, the predicted EV availability and the charging schedule are used to calculate the possible regulating power capacity from EVs for both up regulating power and down regulating power...

  17. Potential for containment leak paths through electrical penetration assemblies under severe accident conditions. [PWR; BWR

    Energy Technology Data Exchange (ETDEWEB)

    Sebrell, W.

    1983-07-01

    The leakage behavior of containments beyond design conditions and knowledge of failure modes is required for evaluation of mitigation strategies for severe accidents, risk studies, emergency preparedness planning, and siting. These studies are directed towards assessing the risk and consequences of severe accidents. An accident sequence analysis conducted on a Boiling Water Reactor (BWR), Mark I (MK I), indicated very high temperatures in the dry-well region, which is the location of the majority of electrical penetration assemblies. Because of the high temperatures, it was postulated in the ORNL study that the sealants would fail and all the electrical penetration assemblies would leak before structural failure would occur. Since other containments had similar electrical penetration assemblies, it was concluded that all containments would experience the same type of failure. The results of this study, however, show that this conclusion does not hold for PWRs because in the worst accident sequence, the long time containment gases stabilize to 350/sup 0/F. BWRs, on the other hand, do experience high dry-well temperatures and have a higher potential for leakage.

  18. An assessment of potential applications with pulsed electric field in wines

    Directory of Open Access Journals (Sweden)

    Drosou Foteini

    2017-01-01

    Full Text Available Pulsed electric fields (PEF is a non-thermal processing technology that uses instantaneous, pulses of high voltage for a short period in the range of milliseconds to microseconds; the application of high intensity electric field on toasted wood chips leads to a quick diffusion of extractable molecules. Currently most PEF studies, in the field of oenology, have been focusing on the application of PEF as a pretreatment of grape musts by examining the microbial inactivation and the enhancement of polyphenol extraction. In this study a post-treatment of wine is introduced as method to enhance the wood flavor in the wine with a green noninvasive technology. Major phenolic aldehydes that have been identified as the characteristic compounds of oak volatile compounds were selected as markers and were analyzed instrumentally to compare the influence of PEF processing to non-treated samples. PEF treated samples brought about higher concentrations of the examined oak compounds in the samples treated with PEF, which may explain the advantages of its application. The modulation of the intensity of the electric field and the period of pulses influenced the concentrations of the volatile phenols that were leached out. Differences found between the assayed treatments indicate that PEF application could be a potential practice for a rapid extraction of volatile compounds from oak.

  19. Electrifying white biotechnology: engineering and economic potential of electricity-driven bio-production.

    Science.gov (United States)

    Harnisch, Falk; Rosa, Luis F M; Kracke, Frauke; Virdis, Bernardino; Krömer, Jens O

    2015-03-01

    The production of fuels and chemicals by electricity-driven bio-production (i.e., using electric energy to drive biosynthesis) holds great promises. However, this electrification of white biotechnology is particularly challenging to achieve because of the different optimal operating conditions of electrochemical and biochemical reactions. In this article, we address the technical parameters and obstacles to be taken into account when engineering microbial bioelectrochemical systems (BES) for bio-production. In addition, BES-based bio-production processes reported in the literature are compared against industrial needs showing that a still large gap has to be closed. Finally, the feasibility of BES bio-production is analysed based on bulk electricity prices. Using the example of lysine production from sucrose, we demonstrate that there is a realistic market potential as cost savings of 8.4 % (in EU) and 18.0 % (in US) could be anticipated, if the necessary yields can be obtained. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Spectral distribution of local field potential responses to electrical stimulation of the retina.

    Science.gov (United States)

    Wong, Yan T; Halupka, Kerry; Kameneva, Tatiana; Cloherty, Shaun L; Grayden, David B; Burkitt, Anthony N; Meffin, Hamish; Shivdasani, Mohit N

    2016-06-01

    Different frequency bands of the local field potential (LFP) have been shown to reflect neuronal activity occurring at varying cortical scales. As such, recordings of the LFP may offer a novel way to test the efficacy of neural prostheses and allow improvement of stimulation strategies via neural feedback. Here we use LFP measurements from visual cortex to characterize neural responses to electrical stimulation of the retina. We aim to show that the LFP is a viable signal that contains sufficient information to optimize the performance of sensory neural prostheses. Clinically relevant electrode arrays were implanted in the suprachoroidal space of one eye in four felines. LFPs were simultaneously recorded in response to stimulation of individual electrodes using penetrating microelectrode arrays from the visual cortex. The frequency response of each electrode was extracted using multi-taper spectral analysis and the uniqueness of the responses was determined via a linear decoder. We found that cortical LFPs are reliably modulated by electrical stimulation of the retina and that the responses are spatially localized. We further characterized the spectral distribution of responses, with maximum information being contained in the low and high gamma bands. Finally, we found that LFP responses are unique to a large range of stimulus parameters (∼40) with a maximum conveyable information rate of 6.1 bits. These results show that the LFP can be used to validate responses to electrical stimulation of the retina and we provide the first steps towards using these responses to provide more efficacious stimulation strategies.

  1. On the magnetic field and the electrical potential generated by bioelectric sources in an anisotropic volume conductor

    NARCIS (Netherlands)

    Peters, M.J.; Elias, P.J.H.

    1988-01-01

    The electrical conductivity in biological tissue is often dependent on the direction of the fibres. In the paper the influence of this anisotropic nature on the electrical potential and magnetic field generated by a current dipole is studied analytically. Three different methods are discussed. The

  2. Assessment of agricultural biomass potential to electricity generation in Riau Province

    Science.gov (United States)

    Papilo, P.; Kusumanto, I.; Kunaifi, K.

    2017-05-01

    Utilization of biomass as a source of electrical power is one potential solution that can be developed in order to increase of the electrification ratio and to Achieve the national energy security. However, now it is still difficult, to Determine the amount of potential energy that can be used as an alternative power generation. Therefore, as a preliminary step to assess the feasibility of biomass development as a power generation source, an analysis of potential resources are required, especially from some of the main commodities, both of residues of agriculture and plantation. This study aims to assessing the potential of biomass-based supply from unutilized resources that can be Obtained from the residues of agricultural and plantations sectors, such as rice straw and rice husk; Dry straw and chaff of rice; corn stalks and cobs; stalks of cassava; and fiber, shell, empty fruit Bunches, kernels and liquid wastes in the palm oil factories. More research is focused on the theoretical energy potential measurements using a statistical approach which has been developed by Biomass Energy Europe (BEE). Results of the assessment has been done and showed that the total theoretical biomass energy that can be produced is equal to 77,466,754.8 Gj year -1. Theoretically, this potential is equivalent to generate electricityof year 21,518,542.8 MWh -1.

  3. Relationship between presynaptic membrane potential and acetylcholine release in synaptosomes from Torpedo electric organ.

    Science.gov (United States)

    Meunier, F M

    1984-01-01

    The membrane potential of purely cholinergic synaptosomes isolated from Torpedo electric organ was monitored with fluorescent carbocyanine dyes. An increased fluorescence was associated with depolarization and a quenching with hyperpolarization. Fluorescence data provided evidence that Torpedo synaptosomes have a membrane potential mainly driven by a K+ diffusion potential and a membrane potential of about -50 mV could be estimated after calibration of fluorescence signals with ionophore antibiotics. The release of acetylcholine (ACh) from Torpedo synaptosomes was monitored continuously by measuring the light emitted by a chemiluminescent method (Israël & Lesbats, 1981 a). Using fluorescence data, the release of ACh was expressed as a function of membrane potential. The relationship between presynaptic potential and transmitter release as determined by biochemical methods at cholinergic nerve endings showed striking similarities to that observed at the squid giant synapse. Several substances were also tested with regard to their depolarizing and releasing properties and it was found that the toxin isolated from the venom of the annelid Glycera convoluta, which induced a large increase in quantal release of transmitter (Manaranche, Thieffry, & Israël, 1980) promoted a depolarization of Torpedo synaptosomes in addition to ACh release. PMID:6207289

  4. The transport of phosphate between the plasma and dialysate compartments in peritoneal dialysis is influenced by an electric potential difference

    DEFF Research Database (Denmark)

    Graff, J; Fugleberg, S; Brahm, J

    1996-01-01

    was not identifiable. Furthermore, it was demonstrated that the electrochemical gradient between plasma water and dialysate favours the diffusive phosphate transport, and both electric and chemical potentials must be taken into account in calculations of the transperitoneal phosphate transport....

  5. SunShot Vision Study: A Comprehensive Analysis of the Potential for U.S. Solar Electricity Generation (Fact Sheet)

    Energy Technology Data Exchange (ETDEWEB)

    2012-06-01

    The SunShot Vision Study provides the most comprehensive assessment to date of the potential for solar technologies to meet a significant share of electricity demand in the United States during the next several decades.

  6. Monitoring Neutrophil-Expressed Cell Surface Esophageal Cancer Related Gene-4 after Severe Burn Injury.

    Science.gov (United States)

    Costantini, Todd W; Coimbra, Raul; Lopez, Nicole E; Lee, Jeanne G; Potenza, Bruce; Smith, Alan; Baird, Andrew; Eliceiri, Brian P

    2015-12-01

    We identified recently esophageal cancer related gene-4 (ECRG4) as a candidate cytokine that is expressed on the surface of quiescent polymorphonuclear leukocytes (PMNs) and shed in response to ex vivo treatment with lipopolysaccharide. To investigate the potential biologic relevance of changes in cell surface ECRG4 in human samples, we performed a pilot study to examine a population of burn patients in whom blood could be analyzed prospectively. We hypothesized that cutaneous burn injury would alter cell surface expression of ECRG4 on PMNs. Patients admitted with more than 20% total burn surface area (TBSA) (n = 10) had blood collected at the time of admission and weekly thereafter. For comparison, blood was obtained from a control group of healthy human volunteers (n = 4). We used flow cytometry to measure changes in ECRG4(+) PMNs from patients during recovery from injury. Esophageal cancer related gene-4 expression at each time point was compared with the patient's clinical status based on a Multiple Organ Dysfunction (MOD) score. Esophageal cancer related gene-4 was detected on the PMN surface of cells collected from healthy volunteers, however, within 48 h of admission after burn injury (n = 10 patients), the number of PMNs with cell surface ECRG4 was decreased. Esophageal cancer related gene-4 expression in PMNs was re-established over the course of patient recovery, unless complications occurred. In this case, the decrease in cell surface ECRG4(+) PMNs preceded the clinical diagnosis of infectious complications and was reflected by increased organ injury scores. From a small sample set, we were able to determine that PMN cell surface ECRG4 expression was decreased after burn injury and returned to baseline during recovery from injury. Although larger studies are needed to define the role of ECRG4 in human PMNs further, this report is the first assessment of cell surface ECRG4 protein in a patient population to support analogous findings in

  7. A sensitive electrochemiluminescence cytosensor for quantitative evaluation of epidermal growth factor receptor expressed on cell surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Tang, Yanjuan; Zhang, Shaolian; Wen, Qingqing; Huang, Hongxing; Yang, Peihui, E-mail: typh@jnu.edu.cn

    2015-06-30

    Highlights: • EGF-cytosensor was used for evaluating EGFR expression level on cell surfaces. • CdSQDs and EGF were coated on magnetic beads (MBs) for ECL-probe. • Good sensitivity was achieved due to the signal amplification of ECL-probe. - Abstract: A sensitive electrochemiluminescence (ECL) strategy for evaluating the epidermal growth factor receptor (EGFR) expression level on cell surfaces was designed by integrating the specific recognition of EGFR expressed on MCF-7 cell surfaces with an epidermal growth factor (EGF)-funtionalized CdS quantum dots (CdSQDs)-capped magnetic bead (MB) probe. The high sensitivity of ECL probe of EGF-funtionalized CdSQD-capped-MB was used for competitive recognition with EGFR expressed on cell surfaces with recombinant EGFR protein. The changes of ECL intensity depended on both the cell number and the expression level of EGFR receptor on cell surfaces. A wide linear response to cells ranging from 80 to 4 × 10{sup 6} cells mL{sup −1} with a detection limit of 40 cells mL{sup −1} was obtained. The EGF-cytosensor was used to evaluate EGFR expression levels on MCF-7 cells, and the average number of EGFR receptor on single MCF-7 cells was 1.35 × 10{sup 5} with the relative standard deviation of 4.3%. This strategy was further used for in-situ and real-time evaluating EGFR receptor expressed on cell surfaces in response to drugs stimulation at different concentration and incubation time. The proposed method provided potential applications in the detection of receptors on cancer cells and anticancer drugs screening.

  8. Expanding the bandwidth of low level photo-electric amplifier using continuous equal-potential shielding

    Science.gov (United States)

    Xie, Kai; Sun, Bin; Shi, Xueyou; Huang, Anfeng; Xi, Ruoyao

    2016-03-01

    For the low level current preamplifier circuits in photo-electric detection, the bandwidth and the low noise characteristics are usually contradictory, because the bandwidth is limited by the high value resistor and its inevitable parasitic capacitances, which together exhibit low-pass characteristics. To expand the bandwidth of low level current amplifier, a novel method is proposed by using continuous equal-potential shielding to eliminate the parasitic capacitances and expand the bandwidth. The shielded resistor contains three layers: the center is high value resistance strip; the top and bottom layers are high conductivity shielding films with same path to center strip. The surrounding electric field energy could be provided by shielding structure instead by high value resistor itself, so the parasitic capacitances would be greatly reduced. In this paper, the principle and the structure of the equal potential shielding are presented, and the possible results are predicted by finite element simulation. The simulation was conducted by the ultra-low current preamplifier with a trans-impedance gain of 50 GΩ. The results indicated that the parasitic capacitances reduced from 1.36×10-2pF to 1.18×10-3pF, meanwhile the bandwidth was expanded from 234.1 Hz to 2.43 kHz with more than 10 times of increase; the rise time decreased from 1.53ms to 0.085ms. The advantage of this method is that the bandwidth could be expanded without extra compensation; meanwhile the shielding layers improve the noise immunity. This method is suitable for the demand of rapid response to low level current in photo-electric detection field, such as scanning tunneling microscopy and laser radar.

  9. An oscillatory component of propagated fluctuation electric potential in lupine shoot

    Directory of Open Access Journals (Sweden)

    Zygmunt Hejnowicz

    2014-01-01

    Full Text Available Application of a drop of auxin solution to a cut surface on the petiole in lupine shoot elicits a travelling pulse of electric potential decrease. This pulse was simultaneously recorded by means of a DC amplifier and band-pass amplifier 0.1-100 Hz, both connected to the same exploring AgCl electrode driven into the stem. The DC record shows a pulse 20-80 mV in height of about 30 s duration at its height with smooth slopes. The band-pass amplifier shows one to a few pairs of spikes (negative and positive whose amplitude is at least of an order lower than that of the DC pulse. These spikes are interpreted as the action potential of certain excitable cells recorded in a "volume conductor". The pulse is interpreted as a wave of cooperative depolarization of excitable and a mass of inexcitable cells.

  10. The market potential for SMES in electric utility applications. Final report

    Energy Technology Data Exchange (ETDEWEB)

    1994-06-01

    Superconducting magnetic energy storage (SMES) is an emerging technology with features that are potentially attractive in electric utility applications. This study evaluates the potential for SMES technology in the generation, transmission, distribution, and use of electric energy; the time frame of the assessment is through the year 2030. Comparisons are made with other technology options, including both commercially available and advanced systems such as various peaking generation technologies, transmission stability improvement technologies, and power quality enhancement devices. The methodology used for this study focused on the needs of the market place, the capabilities of S and the characteristics of the competing technologies. There is widespread interest within utilities for the development of SMES technology, but there is no general consensus regarding the most attractive size. Considerable uncertainty exists regarding the eventual costs and benefits of commercial SMES systems, but general trends have been developed based on current industry knowledge. Results of this analysis indicate that as storage capacity increases, cost increases at a rate faster than benefits. Transmission system applications requiring dynamic storage appear to have the most attractive economics. Customer service applications may be economic in the near term, but improved ride-through capability of end-use equipment may limit the size of this market over time. Other applications requiring greater storage capacity appear to be only marginally economic at best.

  11. Electrical potentiation of the membrane permeabilization by new peptides with anticancer properties.

    Science.gov (United States)

    Lemeshko, Victor V

    2013-03-01

    New polycationic peptides were designed on the basis of 16-mer and 14-mer fragments of the peptide BTM-P1, derived from the Cry11Bb protoxin. The peptides caused mitochondrial, but not red blood cell membrane permeabilization. Conjugation of the cell penetrating hepta-arginine vector to their N- or C-termini through two glycine residues resulted in more active peptides, which also permeabilized the red blood cells with a relatively high plasma membrane potential generated in the presence of valinomycin. The efficiency of the peptides was remarkably higher in the lower ionic strength media. The capability of the plasma membrane permeabilization of the normal red blood cells by the designed conjugated peptides and by known anticancer peptide R7-KLA was also strongly potentiated by the external electrical pulses applied to the cell suspension. These results open the new avenues of the local destruction of solid tumors using the combined "peptide--electrical pulses" synergistic treatment. The designed peptides were active against the human leukemia Jurkat cells but not against the normal wild type CHO cells. Copyright © 2012 Elsevier B.V. All rights reserved.

  12. Enhanced Regenerative Braking Strategies for Electric Vehicles: Dynamic Performance and Potential Analysis

    Directory of Open Access Journals (Sweden)

    Boyi Xiao

    2017-11-01

    Full Text Available A regenerative braking system and hydraulic braking system are used in conjunction in the majority of electric vehicles worldwide. We propose a new regenerative braking distribution strategy that is based on multi-input fuzzy control logic while considering the influences of the battery’s state of charge, the brake strength and the motor speed. To verify the braking performance and recovery economy, this strategy was applied to a battery electric vehicle model and compared with two other improved regenerative braking strategies. The performance simulation was performed using standard driving cycles (NEDC, LA92, and JP1015 and a real-world-based urban cycle in China. The tested braking strategies satisfied the general safety requirements of Europe (as specified in ECE-13H, and the emergency braking scenario and economic potential were tested. The simulation results demonstrated the differences in the braking force distribution performance of these three regenerative braking strategies, the feasibility of the braking methods for the proposed driving cycles and the energy economic potential of the three strategies.

  13. Postfatigue potentiation of the paralyzed soleus muscle: evidence for adaptation with long-term electrical stimulation training

    OpenAIRE

    Shields, Richard K.; Dudley-Javoroski, Shauna; Littmann, Andrew E.

    2006-01-01

    Understanding the torque output behavior of paralyzed muscle has important implications for the use of functional neuromuscular electrical stimulation systems. Postfatigue potentiation is an augmentation of peak muscle torque during repetitive activation after a fatigue protocol. The purposes of this study were 1) to quantify postfatigue potentiation in the acutely and chronically paralyzed soleus and 2) to determine the effect of long-term soleus electrical stimulation training on the potent...

  14. The change of electric potentials in the oral cavity after application of extremely low frequency pulsed magnetic field

    Directory of Open Access Journals (Sweden)

    Piotr Skomro

    2012-12-01

    Full Text Available Electric potentials occurring in the oral cavity deserve attention as they may cause various diseases and subjective feelings, which are very difficult to treat. The aim of this study was to evaluate the electric potentials within the oral cavity in patients with metal fillings and metal prosthetic restorations, after using a pulsed electromagnetic field. The study was carried out on 84 patients. The Viofor JPS Classic device was used in the treatment. It generates a pulsed electromagnetic field with low induction of the extremely low frequency (ELF range. Average values of electric potentials in the preliminary test were about the same in both groups; they were 148.8 mV and 145.5 mV. After another appliance of ELF fields there was found a steady decline in the average value of electric potentials in the study group. This decrease was statistically highly significant, while mean values of electric potentials in the control group were characterized by a slightly upward tendency. The obtained statistically significant reduction of electric potentials in the oral cavity of patients having metal fillings and metal prosthetic restorations, after application of the Viofor JPS Classic device, implies a huge impact of ELF pulsed electromagnetic field on inhibition of electrochemical processes, as well as on inhibition of dental alloy corrosion. 

  15. The contribution of electrical synapses to field potential oscillations in the hippocampal formation.

    Science.gov (United States)

    Posłuszny, Anna

    2014-01-01

    Electrical synapses are a type of cellular membrane junction referred to as gap junctions (GJs). They provide a direct way to exchange ions between coupled cells and have been proposed as a structural basis for fast transmission of electrical potentials between neurons in the brain. For this reason GJs have been regarded as an important component within the neuronal networks that underlie synchronous neuronal activity and field potential oscillations. Initially, GJs appeared to play a particularly key role in the generation of high frequency oscillatory patterns in field potentials. In order to assess the scale of neuronal GJs contribution to field potential oscillations in the hippocampal formation, in vivo and in vitro studies are reviewed here. These investigations have shown that blocking the main neuronal GJs, those containing connexin 36 (Cx36-GJs), or knocking out the Cx36 gene affect field potential oscillatory patterns related to awake active behavior (gamma and theta rhythm) but have no effect on high frequency oscillations occurring during silent wake and sleep. Precisely how Cx36-GJs influence population activity of neurons is more complex than previously thought. Analysis of studies on the properties of transmission through GJ channels as well as Cx36-GJs functioning in pairs of coupled neurons provides some explanations of the specific influence of Cx36-GJs on field potential oscillations. It is proposed here that GJ transmission is strongly modulated by the level of neuronal network activity and changing behavioral states. Therefore, contribution of GJs to field potential oscillatory patterns depends on the behavioral state. I propose here a model, based on large body of experimental data gathered in this field by several authors, in which Cx36-GJ transmission especially contributes to oscillations related to active behavior, where it plays a role in filtering and enhancing coherent signals in the network under high-noise conditions. In contrast

  16. Theoretical aspects of electrical power generation from two-phase flow streaming potentials

    NARCIS (Netherlands)

    Sherwood, J.D.; Xie, Yanbo; van den Berg, Albert; Eijkel, Jan C.T.

    A theoretical analysis of the generation of electrical streaming currents and electrical power by two-phase flow in a rectangular capillary is presented. The injection of a second, non-conducting fluid phase tends to increase the internal electrical resistance of the electrical generator, thereby

  17. Clean Energy Technologies: A Preliminary Inventory of the Potential for Electricity Generation

    Energy Technology Data Exchange (ETDEWEB)

    Bailey, Owen; Worrell, Ernst

    2005-08-03

    The nation's power system is facing a diverse and broad set of challenges. These range from restructuring and increased competitiveness in power production to the need for additional production and distribution capacity to meet demand growth, and demands for increased quality and reliability of power and power supply. In addition, there are growing concerns about emissions from fossil fuel powered generation units and generators are seeking methods to reduce the CO{sub 2} emission intensity of power generation. Although these challenges may create uncertainty within the financial and electricity supply markets, they also offer the potential to explore new opportunities to support the accelerated deployment of cleaner and cost-effective technologies to meet such challenges. The federal government and various state governments, for example, support the development of a sustainable electricity infrastructure. As part of this policy, there are a variety of programs to support the development of ''cleaner'' technologies such as combined heat and power (CHP, or cogeneration) and renewable energy technologies. Energy from renewable energy sources, such as solar, wind, hydro, and biomass, are considered carbon-neutral energy technologies. The production of renewable energy creates no incremental increase in fossil fuel consumption and CO{sub 2} emissions. Electricity and thermal energy production from all renewable resources, except biomass, produces no incremental increase in air pollutants such as nitrogen oxides, sulfur oxides, particulate matter, and carbon monoxide. There are many more opportunities for the development of cleaner electricity and thermal energy technologies called ''recycled'' energy. A process using fossil fuels to produce an energy service may have residual energy waste streams that may be recycled into useful energy services. Recycled energy methods would capture energy from sources that would otherwise

  18. Development of 3-D Mechanical Models of Electric Circuits and Their Effect on Students' Understanding of Electric Potential Difference

    Science.gov (United States)

    Balta, Nuri

    2015-01-01

    Visualizing physical concepts through models is an essential method in many sciences. While students are mostly proficient in handling mathematical aspects of problems, they frequently lack the ability to visualize and interpret abstract physical concepts in a meaningful way. In this paper, initially the electric circuits and related concepts were…

  19. Perspectives on microbial cell surface display in bioremediation.

    Science.gov (United States)

    Saleem, M; Brim, H; Hussain, S; Arshad, M; Leigh, M B; Zia-ul-Hassan

    2008-01-01

    The display of heterologous proteins on the microbial cell surface by means of recombinant DNA biotechnologies has emerged as a novel approach for bioremediation of contaminated sites. Both bacteria and yeasts have been investigated for this purpose. Cell surface expression of specific proteins allows the engineered microorganisms to transport, bio-accumulate and/or detoxify heavy metals as well as to degrade xenobiotics. These otherwise would not be taken up and transformed by the microbial cell. This review focuses on the application of cell surface displays for the enhanced bio-accumulation of heavy metals by metal binding proteins. It also reviews the biodegradation of xenobiotics by enzymes/proteins expressed on microbial cell surfaces.

  20. Resonance line shape, strain and electric potential distributions of composite magnetoelectric sensors

    Directory of Open Access Journals (Sweden)

    Martina Gerken

    2013-06-01

    Full Text Available Multiferroic composite magnetoelectric (ME sensors are based on the elastic coupling of a magnetostrictive phase and a piezoelectric phase. A deformation of the magnetostrictive phase causes strain in the piezoelectric phase and thus an induced voltage. Such sensors may be applied both for static as well as for dynamic magnetic field measurements. Particularly high sensitivities are achieved for operation at a mechanical resonance. Here, the resonance line shape of layered (2-2 composite cantilever ME sensors at the first bending-mode resonance is investigated theoretically. Finite element method (FEM simulations using a linear material model reveal an asymmetric resonance profile and a zero-response frequency for the ME coefficient. Frequency-dependent strain and electric potential distributions inside the magnetoelectric composite are studied for the case of a magnetostrictive-piezoelectric bilayer. It is demonstrated that a positive or a negative voltage may be induced across the piezoelectric layer depending on the position of the neutral plane. The frequency-dependent induced electric potential is investigated for structured cantilevers that exhibit magnetostriction only at specific positions. For static operation an induced voltage is obtained locally at positions with magnetostriction. In addition to this direct effect a resonance-assisted effect is observed for dynamic operation. Magnetostriction in a limited area of the cantilever causes a global vibration of the cantilever. Thus, deformation of the piezoelectric layer and an induced electric potential also occur in areas of the cantilever without magnetostriction. The direct and the resonance-assisted pathway may induce voltages of equal or of opposite sign. The net induced voltage results from the superposition of the two effects. As the resonance-assisted induced voltage changes sign upon passing the resonance frequency, while the direct component is constant, an asymmetric line

  1. The contribution of electrical synapses to field potential oscillations in the hippocampal formation

    Directory of Open Access Journals (Sweden)

    Anna ePosłuszny

    2014-04-01

    Full Text Available Electrical synapses are a type of cellular membrane junction referred to as gap junctions (GJs. GJs have been regarded as an important component within the neuronal networks that underlie synchronous neuronal activity and field potential oscillations. Initially, GJs appeared to play a particularly key role in the generation of high frequency oscillatory patterns in field potentials. In order to assess the scale of neuronal GJs contribution to field potential oscillations in the hippocampal formation, in vivo and in vitro studies are reviewed here. These investigations have shown that blocking the main neuronal GJs, those containing connexin 36 (Cx36-GJs, or knocking out the Cx36 gene affect field potential oscillatory patterns related to awake active behavior (gamma and theta rhythm but have no effect on high frequency oscillations occurring during silent wake and sleep. Precisely how Cx36-GJs influence population activity of neurons is more complex than previously thought. Analysis of studies on the properties of transmission through GJ channels as well as Cx36-GJs functioning in pairs of coupled neurons provides some explanations of the specific influence of Cx36-GJs on field potential oscillations. It is proposed here that GJ transmission is strongly modulated by the level of neuronal network activity and changing behavioral states. Therefore, contribution of GJs to field potential oscillatory patterns depends on the behavioral state. I propose here a model, based on large body of experimental data gathered in this field by several authors, in which Cx36-GJ transmission especially contributes to oscillations related to active behavior, where it plays a role in filtering and enhancing coherent signals in the network under high-noise conditions. In contrast, oscillations related to silent wake or sleep, especially high frequency oscillations, do not require transmission by neuronal GJs.

  2. Potential air quality benefits from increased solar photovoltaic electricity generation in the Eastern United States

    Science.gov (United States)

    Abel, David; Holloway, Tracey; Harkey, Monica; Rrushaj, Arber; Brinkman, Greg; Duran, Phillip; Janssen, Mark; Denholm, Paul

    2018-02-01

    We evaluate how fine particulate matter (PM2.5) and precursor emissions could be reduced if 17% of electricity generation was replaced with solar photovoltaics (PV) in the Eastern United States. Electricity generation is simulated using GridView, then used to scale electricity-sector emissions of sulfur dioxide (SO2) and nitrogen oxides (NOX) from an existing gridded inventory of air emissions. This approach offers a novel method to leverage advanced electricity simulations with state-of-the-art emissions inventories, without necessitating recalculation of emissions for each facility. The baseline and perturbed emissions are input to the Community Multiscale Air Quality Model (CMAQ version 4.7.1) for a full accounting of time- and space-varying air quality changes associated with the 17% PV scenario. These results offer a high-value opportunity to evaluate the reduced-form AVoided Emissions and geneRation Tool (AVERT), while using AVERT to test the sensitivity of results to changing base-years and levels of solar integration. We find that average NOX and SO2 emissions across the region decrease 20% and 15%, respectively. PM2.5 concentrations decreased on average 4.7% across the Eastern U.S., with nitrate (NO3-) PM2.5 decreasing 3.7% and sulfate (SO42-) PM2.5 decreasing 9.1%. In the five largest cities in the region, we find that the most polluted days show the most significant PM2.5 decrease under the 17% PV generation scenario, and that the greatest benefits are accrued to cities in or near the Ohio River Valley. We find summer health benefits from reduced PM2.5 exposure estimated as 1424 avoided premature deaths (95% Confidence Interval (CI): 284 deaths, 2 732 deaths) or a health savings of 13.1 billion (95% CI: 0.6 billion, 43.9 billion) These results highlight the potential for renewable energy as a tool for air quality managers to support current and future health-based air quality regulations.

  3. Impact of local environmental conditions on atmospheric electrical potential gradient measurements

    Science.gov (United States)

    Buzás, Attila; Barta, Veronika; Steinbach, Péter; Bór, József

    2017-04-01

    The atmospheric electrical potential gradient (PG) is a fundamental parameter of the global electric circuit (GEC) which comprises all large scale quasi-static electrical processes occurring in between the surface of the Earth and the lower ionosphere. The observation of PG near the Earth's surface plays a pivotal role in surveying our atmospheric electrical environment. The PG shows high variability in different temporal and spatial scales and it is especially sensitive to local effects. Therefore, obtaining a PG value which represents the general state of the GEC over a larger area rather than various effects due to measuring site-specific local factors is a challenging task. PG measurements are going on in the Széchenyi István Geophysical Observatory (NCK, 47°38' N, 16°43' E) of the Research Centre for Astronomy and Earth Sciences, Hungarian Academy of Sciences near Nagycenk, Hungary since 1961. PG sensors are set up in NCK in an open area surrounded by buildings and trees within 20 m distance. The effect of the changing vegetation on the long-term trend observed in the PG variation at NCK has been subject of debates [1,2,3]. In order to examine the possible bias in the measured PG values due to the relatively close buildings and trees at NCK, two sets of simultaneous PG measurements from two EFM-100 field mills were compared. One field mill was kept at a fixed location while the other was moved to grid points covering the open area around the fixed field mill. The measurement was done in fair weather conditions in summer and was repeated during the winter. The poster demonstrates the performance of this method in surveying the effect of various objects and the state of vegetation on the measured PG values by comparing the measured PG differences to those obtained from electrostatic models calculated by the finite element method using the FEMM 4.2 software package. [1] F. Märcz and R. G. Harrison, 2003, Annales Gephysicae, 21: 2193-2200 [2] F. Märcz and R

  4. Potential air quality benefits from increased solar photovoltaic electricity generation in the Eastern United States

    Energy Technology Data Exchange (ETDEWEB)

    Abel, David; Holloway, Tracey; Harkey, Monica; Rrushaj, Arber; Brinkman, Greg; Duran, Phillip; Janssen, Mark; Denholm, Paul

    2018-02-01

    We evaluate how fine particulate matter (PM2.5) and precursor emissions could be reduced if 17% of electricity generation was replaced with solar photovoltaics (PV) in the Eastern United States. Electricity generation is simulated using GridView, then used to scale electricity-sector emissions of sulfur dioxide (SO2) and nitrogen oxides (NOX) from an existing gridded inventory of air emissions. This approach offers a novel method to leverage advanced electricity simulations with state-of-the-art emissions inventories, without necessitating recalculation of emissions for each facility. The baseline and perturbed emissions are input to the Community Multiscale Air Quality Model (CMAQ version 4.7.1) for a full accounting of time- and space-varying air quality changes associated with the 17% PV scenario. These results offer a high-value opportunity to evaluate the reduced-form AVoided Emissions and geneRation Tool (AVERT), while using AVERT to test the sensitivity of results to changing base-years and levels of solar integration. We find that average NOX and SO2 emissions across the region decrease 20% and 15%, respectively. PM2.5 concentrations decreased on average 4.7% across the Eastern U.S., with nitrate (NO3-) PM2.5 decreasing 3.7% and sulfate (SO42-) PM2.5 decreasing 9.1%. In the five largest cities in the region, we find that the most polluted days show the most significant PM2.5 decrease under the 17% PV generation scenario, and that the greatest benefits are accrued to cities in or near the Ohio River Valley. We find summer health benefits from reduced PM2.5 exposure estimated as 1424 avoided premature deaths (95% Confidence Interval (CI): 284 deaths, 2 732 deaths) or a health savings of $13.1 billion (95% CI: $0.6 billion, $43.9 billion) These results highlight the potential for renewable energy as a tool for air quality managers to support current and future health-based air quality regulations.

  5. Atomic bonding and electrical potential at metal/oxide interfaces, a first principle study

    Science.gov (United States)

    Tea, Eric; Huang, Jianqiu; Li, Guanchen; Hin, Celine

    2017-03-01

    A number of electronic devices involve metal/oxide interfaces in their structure where the oxide layer plays the role of electrical insulator. As the downscaling of devices continues, the oxide thickness can spread over only a few atomic layers, making the role of interfaces prominent on its insulating properties. The prototypical Al/SiO2 metal/oxide interface is investigated using first principle calculations, and the effect of the interfacial atomic bonding is evidenced. It is shown that the interface bonding configuration critically dictates the mechanical and electronic properties of the interface. Oxygen atoms are found to better delimit the oxide boundaries than cations. Interfacial cation-metal bonds allow the metal potential to leak inside the oxide layer, without atomic diffusion, leading to a virtual oxide thinning.

  6. Environmental emission mitigation potential of efficient electrical appliances in Sri Lanka

    Energy Technology Data Exchange (ETDEWEB)

    Shrestha, R.M.; Shrestha, R. [Asian Institute of Technology, Pathumthani (Thailand). Energy Program; Fernando, W.J.L.S. [Sri Lanka Energy Managers` Association, Colombo (Slovakia)

    1998-08-01

    This study assesses the techno-economic potential of selected efficient demand-side appliances to mitigate emission of air pollutants from the power sector of Sri Lanka. The study shows that through the use of the selected efficient appliances a total of about 38 646 GWh (i.e. 18.5% of total electricity generation) and about 25.6% (29 541,000 tons), 34.2% (293,000 tons) and 34.6% (374,000 tons) of the total COsub(2), SOsub(2) and NOsub(x) emission respectively could be avoided during 1997-2015 with the use of the efficient appliances from the technical and national economic perspectives. (author)

  7. Electric and Hybrid Vehicle System Research and Development Project: Hybrid Vehicle Potential Assessment. Volume 1. Summary

    Energy Technology Data Exchange (ETDEWEB)

    Surber, F.T.

    1979-09-30

    The results of investigations conducted under Ce Hybrid Vehicle Potential Assessment Task are reported in 10 volumes. This volume contains an overview of the study and its results. The purpose of the overall study was to determine if the petroleum fuel savings achievable through the use of hybrid electric vehicles is worth the R and D expenditures needed to develop the hybrid vehicles and to determine R and D priorities. It was concluded that by the year 2010 hybrid vehicles could replace 80% of the automotive power that would otherwise be produced from petroleum fuels; the public should not suffer any mobility loss through the use of hybrid vehicles; high initial and life-cycle costs are a limiting factor; and R and D funds should be spent for systems design and the development of low-cost batteries and controllers. (LCL)

  8. Surface Electrical Potentials of Root Cell Plasma Membranes: Implications for Ion Interactions, Rhizotoxicity, and Uptake

    Directory of Open Access Journals (Sweden)

    Yi-Min Wang

    2014-12-01

    Full Text Available Many crop plants are exposed to heavy metals and other metals that may intoxicate the crop plants themselves or consumers of the plants. The rhizotoxicity of heavy metals is influenced strongly by the root cell plasma membrane (PM surface’s electrical potential (ψ0. The usually negative ψ0 is created by negatively charged constituents of the PM. Cations in the rooting medium are attracted to the PM surface and anions are repelled. Addition of ameliorating cations (e.g., Ca2+ and Mg2+ to the rooting medium reduces the effectiveness of cationic toxicants (e.g., Cu2+ and Pb2+ and increases the effectiveness of anionic toxicants (e.g., SeO42− and H2AsO4−. Root growth responses to ions are better correlated with ion activities at PM surfaces ({IZ}0 than with activities in the bulk-phase medium ({IZ}b (IZ denotes an ion with charge Z. Therefore, electrostatic effects play a role in heavy metal toxicity that may exceed the role of site-specific competition between toxicants and ameliorants. Furthermore, ψ0 controls the transport of ions across the PM by influencing both {IZ}0 and the electrical potential difference across the PM from the outer surface to the inner surface (Em,surf. Em,surf is a component of the driving force for ion fluxes across the PM and controls ion-channel voltage gating. Incorporation of {IZ}0 and Em,surf into quantitative models for root metal toxicity and uptake improves risk assessments of toxic metals in the environment. These risk assessments will improve further with future research on the application of electrostatic theory to heavy metal phytotoxicity in natural soils and aquatic environments.

  9. Peripheral electrical stimulation triggered by self-paced detection of motor intention enhances motor evoked potentials.

    Science.gov (United States)

    Niazi, Imran Khan; Mrachacz-Kersting, Natalie; Jiang, Ning; Dremstrup, Kim; Farina, Dario

    2012-07-01

    This paper proposes the development and experimental tests of a self-paced asynchronous brain-computer interfacing (BCI) system that detects movement related cortical potentials (MRCPs) produced during motor imagination of ankle dorsiflexion and triggers peripheral electrical stimulations timed with the occurrence of MRCPs to induce corticospinal plasticity. MRCPs were detected online from EEG signals in eight healthy subjects with a true positive rate (TPR) of 67.15 ± 7.87% and false positive rate (FPR) of 22.05 ±9.07%. The excitability of the cortical projection to the target muscle (tibialis anterior) was assessed before and after the intervention through motor evoked potentials (MEP) using transcranial magnetic stimulation (TMS). The peak of the evoked potential significantly (P=0.02) increased after the BCI intervention by 53 ± 43% (relative to preintervention measure), although the spinal excitability (tested by stretch reflexes) did not change. These results demonstrate for the first time that it is possible to alter the corticospinal projections to the tibialis anterior muscle by using an asynchronous BCI system based on online motor imagination that triggered peripheral stimulation. This type of repetitive proprioceptive feedback training based on self-generated brain signal decoding may be a requirement for purposeful skill acquisition in intact humans and in the rehabilitation of persons with brain damage.

  10. Impact of rock mass temperature on potential power and electricity generation in the ORC installation

    Science.gov (United States)

    Kaczmarczyk, Michał

    2017-11-01

    The basic source of information for determining the temperature distribution in the rock mass and thus the potential for thermal energy contained in geothermal water conversion to electricity, are: temperature measurements in stable geothermic conditions, temperature measurements in unstable conditions, measurements of maximum temperatures at the bottom of the well. Incorrect temperature estimation can lead to errors during thermodynamic parameters calculation and consequently economic viability of the project. The analysis was performed for the geothermal water temperature range of 86-100°C, for dry working fluid R245fa. As a result of the calculations, the data indicate an increase in geothermal power as the geothermal water temperature increases. At 86°C, the potential power is 817.48 kW, increases to 912.20 kW at 88°C and consequently to 1 493.34 kW at 100°C. These results are not surprising, but show a scale of error in assessing the potential that can result improper interpretation of the rock mass and geothermal waters temperature.

  11. The potential of electricity transmission corridors in forested areas as bumblebee habitat.

    Science.gov (United States)

    Hill, Bruce; Bartomeus, Ignasi

    2016-11-01

    Declines in pollinator abundance and diversity are not only a conservation issue, but also a threat to crop pollination. Maintained infrastructure corridors, such as those containing electricity transmission lines, are potentially important wild pollinator habitat. However, there is a lack of evidence comparing the abundance and diversity of wild pollinators in transmission corridors with other important pollinator habitats. We compared the diversity of a key pollinator group, bumblebees (Bombus spp.), between transmission corridors and the surrounding semi-natural and managed habitat types at 10 sites across Sweden's Uppland region. Our results show that transmission corridors have no impact on bumblebee diversity in the surrounding area. However, transmission corridors and other maintained habitats such as roadsides have a level of bumblebee abundance and diversity comparable to semi-natural grasslands and host species that are important for conservation and ecosystem service provision. Under the current management regime, transmission corridors already provide valuable bumblebee habitat, but given that host plant density is the main determinant of bumblebee abundance, these areas could potentially be enhanced by establishing and maintaining key host plants. We show that in northern temperate regions the maintenance of transmission corridors has the potential to contribute to bumblebee conservation and the ecosystem services they provide.

  12. Utilization of the Flexibility Potential of Electric Vehicles - an Alternative to Distribution Grid Reinforcements.

    OpenAIRE

    Ager-Hanssen, Siri Bruskeland; Myhre, Siri Olimb

    2015-01-01

    Today, the transport sector accounts for a large share of global emissions. Electric vehicles have many environmental advantages compared to conventional petrol vehicles. Hence, if electric vehicles can replace petrol vehicles, the transportation sector's total emissions can be significantly reduced. In Norway, due to policy incentives, it is expected that the number of electric vehicles will increase considerably in the near future. Despite the great advantages of electric vehicles, large pe...

  13. Synthesis of novel electrically conducting polymers: Potential conducting Langmuir-Blodgett films and conducting polymers on defined surfaces

    Science.gov (United States)

    Zimmer, Hans

    1993-01-01

    Based on previous results involving thiophene derived electrically conducting polymers in which it was shown that thiophene, 3-substituted thiophenes, furans, and certain oligomers of these compounds showed electrical conductivity after polymerization. The conductivity was in the order of up to 500 S/cm. In addition, these polymers showed conductivity without being doped and most of all they were practically inert toward ambient conditions. They even could be used in aqueous media. With these findings as a guide, a number of 3-long-chain-substituted thiophenes and 1-substituted-3-long-chain substituted pyrrols were synthesized as monomers for potential polymeric electrically conducting Langmuir-Blodgett films.

  14. Effect of conductivity variations within the electric double layer on the streaming potential estimation in narrow fluidic confinements.

    Science.gov (United States)

    Das, Siddhartha; Chakraborty, Suman

    2010-07-06

    In this article, we investigate the implications of ionic conductivity variations within the electrical double layer (EDL) on the streaming potential estimation in pressure-driven fluidic transport through narrow confinements. Unlike the traditional considerations, we do not affix the ionic conductivities apriori by employing preset values of dimensionless parameters (such as the Dukhin number) to estimate the streaming potential. Rather, utilizing the Gouy-Chapman-Grahame model for estimating the electric potential and charge density distribution within the Stern layer, we first quantify the Stern layer electrical conductivity as a function of the zeta potential and other pertinent parameters quantifying the interaction of the ionic species with the charged surface. Next, by invoking the Boltzmann model for cationic and anionic distribution within the diffuse layer, we obtain the diffuse layer electrical conductivity. On the basis of these two different conductivities pertaining to the two different portions of the EDL as well as the bulk conductivity, we define two separate Dukhin numbers that turn out to be functions of the dimensionless zeta potential and the channel height to Debye length ratio. We derive analytical expressions for the streaming potential as a function of the fundamental governing parameters, considering the above. The results reveal interesting and significant deviations between the streaming potential predictions from the present considerations against the corresponding predictions from the classical considerations in which electrochemically consistent estimates of variable EDL conductivity are not traditionally accounted for. In particular, it is revealed that the variations of streaming potential with zeta potential are primarily determined by the competing effects of EDL electromigration and ionic advection. Over low and high zeta potential regimes, the Stern layer and diffuse layer conductivities predominantly dictate the streaming

  15. A Hybrid Geothermal Energy Conversion Technology - A Potential Solution for Production of Electricity from Shallow Geothermal Resources

    OpenAIRE

    Garapati, Nagasree; Adams, Benjamin M.; Bielicki, Jeffrey M.; Schädle, Philippe; Randolph, Jimmy B.; Kuehn, Thomas H.; Saar, Martin O.

    2017-01-01

    Geothermal energy has been successfully employed in Switzerland for more than a century for direct use but presently there is no electricity being produced from geothermal sources. After the nuclear power plant catastrophe in Fukushima, Japan, the Swiss Federal Assembly decided to gradually phase out the Swiss nuclear energy program. Deep geothermal energy is a potential resource for clean and nearly CO2-free electricity production that can supplant nuclear power in Switzerland and worldwide....

  16. A Methodology for Calculating EGS Electricity Generation Potential Based on the Gringarten Model for Heat Extraction From Fractured Rock

    Energy Technology Data Exchange (ETDEWEB)

    Augustine, Chad

    2017-05-01

    Existing methodologies for estimating the electricity generation potential of Enhanced Geothermal Systems (EGS) assume thermal recovery factors of 5% or less, resulting in relatively low volumetric electricity generation potentials for EGS reservoirs. This study proposes and develops a methodology for calculating EGS electricity generation potential based on the Gringarten conceptual model and analytical solution for heat extraction from fractured rock. The electricity generation potential of a cubic kilometer of rock as a function of temperature is calculated assuming limits on the allowed produced water temperature decline and reservoir lifetime based on surface power plant constraints. The resulting estimates of EGS electricity generation potential can be one to nearly two-orders of magnitude larger than those from existing methodologies. The flow per unit fracture surface area from the Gringarten solution is found to be a key term in describing the conceptual reservoir behavior. The methodology can be applied to aid in the design of EGS reservoirs by giving minimum reservoir volume, fracture spacing, number of fractures, and flow requirements for a target reservoir power output. Limitations of the idealized model compared to actual reservoir performance and the implications on reservoir design are discussed.

  17. Health effects three years after potential exposure to the toxic contaminants of an electrical transformer fire.

    Science.gov (United States)

    Fitzgerald, E F; Weinstein, A L; Youngblood, L G; Standfast, S J; Melius, J M

    1989-01-01

    A medical surveillance program has been established for 482 persons who were potentially exposed to polychlorinated biphenyls (PCBs), dibenzo-p-dioxins, and dibenzofurans from an electrical transformer fire in a Binghamton, NY office building in 1981. Vital Record and Cancer Registry data, medical records, and mail questionnaires were used to assess mortality, symptomatology, cancer incidence, and reproductive events through 1984. The numbers of deaths, cancers, fetal deaths, and infants with low birth weight or congenital malformations were similar to those expected on the basis of age- and sex-specific rates for upstate New York and other comparison populations. Two suicides were observed compared with 0.31 expected, but the difference was not statistically significant. After adjustment for possible confounders, persons with the greatest degree of potential exposure were significantly more likely than those with less exposure to report unexplained weight loss (relative risk [RR] = 12.80), muscle pain (RR = 5.07), frequent coughing (RR = 4.14), skin color changes (RR = 3.49), and nervousness or sleep problems (RR = 3.19). The possibility of recall bias and the intervening effects of stress, however, weaken the conclusion that toxic chemicals caused the symptomatology. Exposure-related systemic disorders, e.g., chloracne or peripheral neuropathy, were not diagnosed by personal physicians; however, some persons refused to release their medical records because of ongoing litigation. The findings are consistent with those of our earlier assessment.

  18. The Effect of Electric Field on RbCl Asymmetric Gaussian Potential Quantum Well Qubit

    Science.gov (United States)

    Xiao, Jing-Lin

    2016-01-01

    We calculate the time evolution and the coordinate change of the quantum mechanical electronic state by using variational method of Pekar type (VMPT) in the presence of strong electron-LO-phonon coupling. The electron is confined in an asymmetric Gaussian potential quantum well (AGPQW) subjected to an applied electric field. The eigenenergies and the eigenfunctions of the ground and the first excited states (GFES) are calculated. A single qubit can be realized in this two-level quantum system. The electron's probability density oscillates in the AGPQW with a certain period of T 0 = 22.511 fs when the electron is in the superposition state of the GFES. We show that due to the presence of the asymmetrical Gaussian potential in the growth direction of the QW, the electron's probability density shows one peak in the range of the coordinate z > 0, whereas it equals to zero in the range of z 0.24 nm. It has a minimum when R = 0.24 nm.

  19. Health effects three years after potential exposure to the toxic contaminants of an electrical transformer fire

    Energy Technology Data Exchange (ETDEWEB)

    Fitzgerald, E.F.; Weinstein, A.L.; Youngblood, L.G.; Standfast, S.J.; Melius, J.M. (New York State Department of Health, Albany (USA))

    1989-07-01

    A medical surveillance program has been established for 482 persons who were potentially exposed to polychlorinated biphenyls (PCBs), dibenzo-p-dioxins, and dibenzofurans from an electrical transformer fire in a Binghamton, NY office building in 1981. Vital Record and Cancer Registry data, medical records, and mail questionnaires were used to assess mortality, symptomatology, cancer incidence, and reproductive events through 1984. The numbers of deaths, cancers, fetal deaths, and infants with low birth weight or congenital malformations were similar to those expected on the basis of age- and sex-specific rates for upstate New York and other comparison populations. Two suicides were observed compared with 0.31 expected, but the difference was not statistically significant. After adjustment for possible confounders, persons with the greatest degree of potential exposure were significantly more likely than those with less exposure to report unexplained weight loss (relative risk (RR) = 12.80), muscle pain (RR = 5.07), frequent coughing (RR = 4.14), skin color changes (RR = 3.49), and nervousness or sleep problems (RR = 3.19). The possibility of recall bias and the intervening effects of stress, however, weaken the conclusion that toxic chemicals caused the symptomatology. Exposure-related systemic disorders, e.g., chloracne or peripheral neuropathy, were not diagnosed by personal physicians; however, some persons refused to release their medical records because of ongoing litigation. The findings are consistent with those of our earlier assessment.

  20. The Mouse C2C12 Myoblast Cell Surface N-Linked Glycoproteome

    Science.gov (United States)

    Gundry, Rebekah L.; Raginski, Kimberly; Tarasova, Yelena; Tchernyshyov, Irina; Bausch-Fluck, Damaris; Elliott, Steven T.; Boheler, Kenneth R.; Van Eyk, Jennifer E.; Wollscheid, Bernd

    2009-01-01

    Endogenous regeneration and repair mechanisms are responsible for replacing dead and damaged cells to maintain or enhance tissue and organ function, and one of the best examples of endogenous repair mechanisms involves skeletal muscle. Although the molecular mechanisms that regulate the differentiation of satellite cells and myoblasts toward myofibers are not fully understood, cell surface proteins that sense and respond to their environment play an important role. The cell surface capturing technology was used here to uncover the cell surface N-linked glycoprotein subproteome of myoblasts and to identify potential markers of myoblast differentiation. 128 bona fide cell surface-exposed N-linked glycoproteins, including 117 transmembrane, four glycosylphosphatidylinositol-anchored, five extracellular matrix, and two membrane-associated proteins were identified from mouse C2C12 myoblasts. The data set revealed 36 cluster of differentiation-annotated proteins and confirmed the occupancy for 235 N-linked glycosylation sites. The identification of the N-glycosylation sites on the extracellular domain of the proteins allowed for the determination of the orientation of the identified proteins within the plasma membrane. One glycoprotein transmembrane orientation was found to be inconsistent with Swiss-Prot annotations, whereas ambiguous annotations for 14 other proteins were resolved. Several of the identified N-linked glycoproteins, including aquaporin-1 and β-sarcoglycan, were found in validation experiments to change in overall abundance as the myoblasts differentiate toward myotubes. Therefore, the strategy and data presented shed new light on the complexity of the myoblast cell surface subproteome and reveal new targets for the clinically important characterization of cell intermediates during myoblast differentiation into myotubes. PMID:19656770

  1. Identification of novel tumor-associated cell surface sialoglycoproteins in human glioblastoma tumors using quantitative proteomics.

    Directory of Open Access Journals (Sweden)

    François Autelitano

    Full Text Available Glioblastoma multiform (GBM remains clinical indication with significant "unmet medical need". Innovative new therapy to eliminate residual tumor cells and prevent tumor recurrences is critically needed for this deadly disease. A major challenge of GBM research has been the identification of novel molecular therapeutic targets and accurate diagnostic/prognostic biomarkers. Many of the current clinical therapeutic targets of immunotoxins and ligand-directed toxins for high-grade glioma (HGG cells are surface sialylated glycoproteins. Therefore, methods that systematically and quantitatively analyze cell surface sialoglycoproteins in human clinical tumor samples would be useful for the identification of potential diagnostic markers and therapeutic targets for malignant gliomas. In this study, we used the bioorthogonal chemical reporter strategy (BOCR in combination with label-free quantitative mass spectrometry (LFQ-MS to characterize and accurately quantify the individual cell surface sialoproteome in human GBM tissues, in fetal, adult human astrocytes, and in human neural progenitor cells (NPCs. We identified and quantified a total of 843 proteins, including 801 glycoproteins. Among the 843 proteins, 606 (72% are known cell surface or secreted glycoproteins, including 156 CD-antigens, all major classes of cell surface receptor proteins, transporters, and adhesion proteins. Our findings identified several known as well as new cell surface antigens whose expression is predominantly restricted to human GBM tumors as confirmed by microarray transcription profiling, quantitative RT-PCR and immunohistochemical staining. This report presents the comprehensive identification of new biomarkers and therapeutic targets for the treatment of malignant gliomas using quantitative sialoglycoproteomics with clinically relevant, patient derived primary glioma cells.

  2. The Anisotropic Aphid: Three-Dimensional Induction Modeling of Electrical Texture with Mixed Potentials

    Science.gov (United States)

    Weiss, C. J.

    2014-12-01

    At the macroscopic scale, where the e-folding distance of low-frequency electromagnetic fields in conductive geomaterials is much larger than the size of organized heterogeneities such as fracture sets or laminations that constitute the geologic texture therein, electrical properties can be conveniently approximated by a generalized 3x3 tensor σ. Less convenient, however, are the algorithmic consequences of this approximation in electromagnetic modeling of 3D induction methods for geophysical exploration. Previous efforts at modelling generalized anisotropy with finite differences on a staggered Cartesian grid (e.g. Weiss and Newman, 2002; Wang and Fang, 2001) are posed in terms of the electric field with its governing "curl-curl" equation and well-documented null-space issues at low induction numbers. In contrast, Weiss (2013) proposed an alternate full-physics formulation in terms of Lorenz-gauged magentic vector A and electric scalar Φ potentials (Project APhiD) that eliminates the troublesome curl-curl operator, with ultrabroadband examples drawn from geologies with scalar, isotropic conductivity over the frequency range 10-2-1010 Hz. Here, the anisotropic theory presented in Weiss (2013) is implemented with finite differences on a Cartesian grid. Briefly stated, in this theoretical approach the conductivity tensor σ is split in terms of a rotationally-invariant isotropic conductivity σ* = ⅓ Tr(σ) and the residual σ - σ*I. This splitting decomposes the resulting finite difference coefficient matrix K into the sum Kiso + Kaniso, where the Kiso term is the coefficient matrix for the isotropic medium σ*, thus enabling reuse of the various routines previously developed for computing matrix coefficients in the isotropic case. Treatment of anisotropy is algorithmically therefore restricted to computing the coefficients in the sparse matrix Kaniso consisting of simple inner products of (σ - σ*I) · (A-∇Φ) and their divergence. In keeping with the

  3. Respiratory-gated electrical impedance tomography: a potential technique for quantifying stroke volume

    Science.gov (United States)

    Arshad, Saaid H.; Murphy, Ethan K.; Halter, Ryan J.

    2016-03-01

    Telemonitoring is becoming increasingly important as the proportion of the population living with cardiovascular disease (CVD) increases. Currently used health parameters in the suite of telemonitoring tools lack the sensitivity and specificity to accurately predict heart failure events, forcing physicians to play a reactive versus proactive role in patient care. A novel cardiac output (CO) monitoring device is proposed that leverages a custom smart phone application and a wearable electrical impedance tomography (EIT) system. The purpose of this work is to explore the potential of using respiratory-gated EIT to quantify stroke volume (SV) and assess its feasibility using real data. Simulations were carried out using the 4D XCAT model to create anatomically realistic meshes and electrical conductivity profiles representing the human thorax and the intrathoracic tissue. A single 5-second period respiration cycle with chest/lung expansion was modeled with end-diastole (ED) and end-systole (ES) heart volumes to evaluate how effective EIT-based conductivity changes represent clinically significant differences in SV. After establishing a correlation between conductivity changes and SV, the applicability of the respiratory-gated EIT was refined using data from the PhysioNet database to estimate the number of useful end-diastole (ED) and end-systole (ES) heart events attained over a 3.3 minute period. The area associated with conductivity changes was found to correlate to SV with a correlation coefficient of 0.92. A window of 12.5% around peak exhalation was found to be the optimal phase of the respiratory cycle from which to record EIT data. Within this window, ~47 useable ED and ES were found with a standard deviation of 28 using 3.3 minutes of data for 20 patients.

  4. A cell-surface phylome for African trypanosomes.

    Science.gov (United States)

    Jackson, Andrew P; Allison, Harriet C; Barry, J David; Field, Mark C; Hertz-Fowler, Christiane; Berriman, Matthew

    2013-01-01

    The cell surface of Trypanosoma brucei, like many protistan blood parasites, is crucial for mediating host-parasite interactions and is instrumental to the initiation, maintenance and severity of infection. Previous comparisons with the related trypanosomatid parasites T. cruzi and Leishmania major suggest that the cell-surface proteome of T. brucei is largely taxon-specific. Here we compare genes predicted to encode cell surface proteins of T. brucei with those from two related African trypanosomes, T. congolense and T. vivax. We created a cell surface phylome (CSP) by estimating phylogenies for 79 gene families with putative surface functions to understand the more recent evolution of African trypanosome surface architecture. Our findings demonstrate that the transferrin receptor genes essential for bloodstream survival in T. brucei are conserved in T. congolense but absent from T. vivax and include an expanded gene family of insect stage-specific surface glycoproteins that includes many currently uncharacterized genes. We also identify species-specific features and innovations and confirm that these include most expression site-associated genes (ESAGs) in T. brucei, which are absent from T. congolense and T. vivax. The CSP presents the first global picture of the origins and dynamics of cell surface architecture in African trypanosomes, representing the principal differences in genomic repertoire between African trypanosome species and provides a basis from which to explore the developmental and pathological differences in surface architectures. All data can be accessed at: http://www.genedb.org/Page/trypanosoma_surface_phylome.

  5. Beyond the cell surface: new mechanisms of receptor function.

    Science.gov (United States)

    Ibáñez, Carlos F

    2010-05-21

    The text book view of cell surface receptors depicts them at the top of a vertical chain of command that starts with ligand binding and proceeds in a lineal fashion towards the cell nucleus. Although pedagogically useful, this view is incomplete and recent findings suggest that the extracellular domain of cell surface receptors can be a transmitter as much as a receiver in intercellular communication. GFRalpha1 is a GPI-anchored receptor for GDNF (glial cell line-derived neurotrophic factor), a neuronal growth factor with widespread functions in the developing and adult nervous system. GFRalpha1 partners with transmembrane proteins, such as the receptor tyrosine kinase RET or the cell adhesion molecule NCAM, for intracellular transmission of the GDNF signal. In addition to this canonical role, GFRalpha1 can also engage in horizontal interactions and thereby modify the function of other cell surface components. GFRalpha1 can also function as a ligand-induced adhesion cell molecule, mediating homophilic cell-cell interactions in response to GDNF. Finally, GFRalpha1 can also be released from the cell surface and act at a distance as a soluble factor together with its ligand. This plethora of unconventional mechanisms is likely to be a feature common to several other receptors and considerably expands our view of cell surface receptor function. 2010 Elsevier Inc. All rights reserved.

  6. Expanding the diversity of unnatural cell surface sialic acids

    Energy Technology Data Exchange (ETDEWEB)

    Luchansky, Sarah J.; Goon, Scarlett; Bertozzi, Carolyn R.

    2003-10-30

    Novel chemical reactivity can be introduced onto cell surfaces through metabolic oligosaccharide engineering. This technique exploits the substrate promiscuity of cellular biosynthetic enzymes to deliver unnatural monosaccharides bearing bioorthogonal functional groups into cellular glycans. For example, derivatives of N-acetylmannosamine (ManNAc) are converted by the cellular biosynthetic machinery into the corresponding sialic acids and subsequently delivered to the cell surface in the form of sialoglycoconjugates. Analogs of N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) are also metabolized and incorporated into cell surface glycans, likely through the sialic acid and GalNAc salvage pathways, respectively. Furthermore, GlcNAc analogs can be incorporated into nucleocytoplasmic proteins in place of {beta}-O-GlcNAc residues. These pathways have been exploited to integrate unique electrophiles such as ketones and azides into the target glycoconjugate class. These functional groups can be further elaborated in a chemoselective fashion by condensation with hydrazides and by Staudinger ligation, respectively, thereby introducing detectable probes onto the cell. In conclusion, sialic acid derivatives are efficient vehicles for delivery of bulky functional groups to cell surfaces and masking of their hydroxyl groups improves their cellular uptake and utilization. Furthermore, the successful introduction of photoactivatable aryl azides into cell surface glycans opens up new avenues for studying sialic acid-binding proteins and elucidating the role of sialic acid in essential processes such as signaling and cell adhesion.

  7. [Research on carbon reduction potential of electric vehicles for low-carbon transportation and its influencing factors].

    Science.gov (United States)

    Shi, Xiao-Qing; Li, Xiao-Nuo; Yang, Jian-Xin

    2013-01-01

    Transportation is the key industry of urban energy consumption and carbon emissions. The transformation of conventional gasoline vehicles to new energy vehicles is an important initiative to realize the goal of developing low-carbon city through energy saving and emissions reduction, while electric vehicles (EV) will play an important role in this transition due to their advantage in energy saving and lower carbon emissions. After reviewing the existing researches on energy saving and emissions reduction of electric vehicles, this paper analyzed the factors affecting carbon emissions reduction. Combining with electric vehicles promotion program in Beijing, the paper analyzed carbon emissions and reduction potential of electric vehicles in six scenarios using the optimized energy consumption related carbon emissions model from the perspective of fuel life cycle. The scenarios included power energy structure, fuel type (energy consumption per 100 km), car type (CO2 emission factor of fuel), urban traffic conditions (speed), coal-power technologies and battery type (weight, energy efficiency). The results showed that the optimized model was able to estimate carbon emissions caused by fuel consumption more reasonably; electric vehicles had an obvious restrictive carbon reduction potential with the fluctuation of 57%-81.2% in the analysis of six influencing factors, while power energy structure and coal-power technologies play decisive roles in life-cycle carbon emissions of electric vehicles with the reduction potential of 78.1% and 81.2%, respectively. Finally, some optimized measures were proposed to reduce transport energy consumption and carbon emissions during electric vehicles promotion including improving energy structure and coal technology, popularizing energy saving technologies and electric vehicles, accelerating the battery R&D and so on. The research provides scientific basis and methods for the policy development for the transition of new energy vehicles

  8. Numerical Computation of Electric Field and Potential Along Silicone Rubber Insulators Under Contaminated and Dry Band Conditions

    Science.gov (United States)

    Arshad; Nekahi, A.; McMeekin, S. G.; Farzaneh, M.

    2016-09-01

    Electrical field distribution along the insulator surface is considered one of the important parameters for the performance evaluation of outdoor insulators. In this paper numerical simulations were carried out to investigate the electric field and potential distribution along silicone rubber insulators under various polluted and dry band conditions. Simulations were performed using commercially available simulation package Comsol Multiphysics based on the finite element method. Various pollution severity levels were simulated by changing the conductivity of pollution layer. Dry bands of 2 cm width were inserted at the high voltage end, ground end, middle part, shed, sheath, and at the junction of shed and sheath to investigate the effect of dry band location and width on electric field and potential distribution. Partial pollution conditions were simulated by applying pollution layer on the top and bottom surface respectively. It was observed from the simulation results that electric field intensity was higher at the metal electrode ends and at the junction of dry bands. Simulation results showed that potential distribution is nonlinear in the case of clean and partially polluted insulator and linear for uniform pollution layer. Dry band formation effect both potential and electric field distribution. Power dissipated along the insulator surface and the resultant heat generation was also studied. The results of this study could be useful in the selection of polymeric insulators for contaminated environments.

  9. The Potential Economic Impact of Electricity Restructuring in the State of Oklahoma: Phase II Report

    Energy Technology Data Exchange (ETDEWEB)

    Hadley, SW

    2001-10-30

    Because of the recent experiences of several states undergoing restructuring (e.g., higher prices, greater volatility, lower reliability), concerns have been raised in states currently considering restructuring as to whether their systems are equally vulnerable. Factors such as local generation costs, transmission constraints, market concentration, and market design can all play a role in the success or failure of the market. These factors along with the mix of generation capacity supplying the state will influence the relative prices paid by consumers. The purpose of this project is to provide a model and process to evaluate the potential price and economic impacts of restructuring the Oklahoma electric industry. The Phase I report concentrated on providing an analysis of the Oklahoma system in the near-term, using only present generation resources and customer demands. This Phase II study analyzed the Oklahoma power market in 2010, incorporating the potential of new generation resources and customer responses. Five key findings of this Phase II were made: (1) Projected expansion in generating capacity exceeds by over 3,000 MW the demands within the state plus the amount that could be exported with the current transmission system. (2) Even with reduced new plant construction, most new plants could lose money (although residential consumers would see lower rates) unless they have sufficient market power to raise their prices without losing significant market share (Figure S-1). (3) If new plants can raise prices to stay profitable, existing low-cost coal and hydro plants will have very high profits. Average prices to customers could be 5% to 25% higher than regulated rates (Figure S-1). If the coal and hydro plants are priced at cost-based rates (through long-term contracts or continued regulation) while all other plants use market-based rates then prices are lower. (4) Customer response to real-time prices can lower the peak capacity requirements by around 9

  10. A combined vector potential-scalar potential method for FE computation of 3D magnetic fields in electrical devices with iron cores

    Science.gov (United States)

    Wang, R.; Demerdash, N. A.

    1991-01-01

    A method of combined use of magnetic vector potential based finite-element (FE) formulations and magnetic scalar potential (MSP) based formulations for computation of three-dimensional magnetostatic fields is introduced. In this method, the curl-component of the magnetic field intensity is computed by a reduced magnetic vector potential. This field intensity forms the basic of a forcing function for a global magnetic scalar potential solution over the entire volume of the region. This method allows one to include iron portions sandwiched in between conductors within partitioned current-carrying subregions. The method is most suited for large-scale global-type 3-D magnetostatic field computations in electrical devices, and in particular rotating electric machinery.

  11. Mediation of rapid electrical, metabolic, transpirational, and photosynthetic changes by factors released from wounds. I. variation potentials and putative action potentials in intact plants

    Science.gov (United States)

    S.J. Barres; T.J.Sambeek Perry; Barbara G. Pickard

    1976-01-01

    Damaging representative plants from five angiosperm families by heating or crushing a small portion of a single leaf results in an electrical change which may spread throughout the shoot. In Mimosa similar changes have previously been identified as variation potentials.Except in one of the five plants, a variation...

  12. Recent Insights into Cell Surface Heparan Sulphate Proteoglycans and Cancer

    DEFF Research Database (Denmark)

    Couchman, John R; Multhaupt, Hinke; Sanderson, Ralph D

    2016-01-01

    A small group of cell surface receptors are proteoglycans, possessing a core protein with one or more covalently attached glycosaminoglycan chains. They are virtually ubiquitous and their chains are major sites at which protein ligands of many types interact. These proteoglycans can signal...... and regulate important cell processes, such as adhesion, migration, proliferation, and differentiation. Since many protein ligands, such as growth factors, morphogens, and cytokines, are also implicated in tumour progression, it is increasingly apparent that cell surface proteoglycans impact tumour cell...... behaviour. Here, we review some recent advances, emphasising that many tumour-related functions of proteoglycans are revealed only after their modification in processes subsequent to synthesis and export to the cell surface. These include enzymes that modify heparan sulphate structure, recycling of whole...

  13. Cell surface engineering of industrial microorganisms for biorefining applications.

    Science.gov (United States)

    Tanaka, Tsutomu; Kondo, Akihiko

    2015-11-15

    In order to decrease carbon emissions and negative environmental impacts of various pollutants, biofuel/biochemical production should be promoted for replacing fossil-based industrial processes. Utilization of abundant lignocellulosic biomass as a feedstock has recently become an attractive option. In this review, we focus on recent efforts of cell surface display using industrial microorganisms such as Escherichia coli and yeast. Cell surface display is used primarily for endowing cellulolytic activity on the host cells, and enables direct fermentation to generate useful fuels and chemicals from lignocellulosic biomass. Cell surface display systems are systematically summarized, and the drawbacks/perspectives as well as successful application of surface display for industrial biotechnology are discussed. Copyright © 2015 Elsevier Inc. All rights reserved.

  14. Engineering Novel and Improved Biocatalysts by Cell Surface Display

    Science.gov (United States)

    Smith, Mason R.; Khera, Eshita; Wen, Fei

    2017-01-01

    Biocatalysts, especially enzymes, have the ability to catalyze reactions with high product selectivity, utilize a broad range of substrates, and maintain activity at low temperature and pressure. Therefore, they represent a renewable, environmentally friendly alternative to conventional catalysts. Most current industrial-scale chemical production processes using biocatalysts employ soluble enzymes or whole cells expressing intracellular enzymes. Cell surface display systems differ by presenting heterologous enzymes extracellularly, overcoming some of the limitations associated with enzyme purification and substrate transport. Additionally, coupled with directed evolution, cell surface display is a powerful platform for engineering enzymes with enhanced properties. In this review, we will introduce the molecular and cellular principles of cell surface display and discuss how it has been applied to engineer enzymes with improved properties as well as to develop surface-engineered microbes as whole-cell biocatalysts. PMID:29056821

  15. To use the full potential of electric metering - a step towards a more energy effective Sweden; Att utnyttja elmaetarnas potential - ett steg mot ett energieffektivare Sverige

    Energy Technology Data Exchange (ETDEWEB)

    Fonter, Gina; Wiberg, Maria (Royal Inst. of Technology, Stockholm (Sweden))

    2010-04-15

    consumption can be reduced by up to 15 percent by using feedback. As a safeguard against future legislative demands over 50 percent of the household have been provided with meters that can handle hourly metering. However, hourly metering is not used to a great extent due to a lack of incentives for the distributors to make use of the hourly metering values. Furthermore not all meters have communication infrastructure that can manage daily collection of values. By changing the current demand of daily collection of values it is possible to employ hourly metering for 74 percent of the households. In addition, propositions of introducing a new metering standard speaks against further investments in the existing metering systems. The metering systems' ability to reflect the short term electricity balance for the consumers is a prerequisite for normal price mechanisms to maintain the security of supply. For that reason, there is a need of metering values with higher resolution in order to indirectly, i.e. by more dynamic tariffs, influence the customer to use less electricity during high load periods. By using hourly metering to the extent made possible with the existing metering systems, this type of service could be offered to a part of the Swedish households. Currently, only the electricity distributors have access to the metering values in the metering systems. This situation limits the business potential that arises from the development of metering services. The business potential is also limited by the fact that the possible savings from electricity conservation are small. Innovation and investments in metering services world be promoted by making metering data accessible to more stakeholders. The development would also be positively affected by higher electricity prices, institutional means of control that promote energy services, and an increasing awareness among consumers. A number of functions that will be important to address the changes facing the electricity

  16. In vivo study of transepithelial potential difference (TEPD) in proximal convoluted tubules of rat kidney by synchronization modulation electric field.

    Science.gov (United States)

    Clausell, Mathis; Fang, Zhihui; Chen, Wei

    2014-07-01

    Synchronization modulation (SM) electric field has been shown to effectively activate function of Na(+)/K(+) pumps in various cells and tissues, including skeletal muscle cells, cardiomyocyte, monolayer of cultured cell line, and peripheral blood vessels. We are now reporting the in vivo studies in application of the SM electric field to kidney of living rats. The field-induced changes in the transepithelial potential difference (TEPD) or the lumen potential from the proximal convoluted tubules were monitored. The results showed that a short time (20 s) application of the SM electric field can significantly increase the magnitude of TEPD from 1-2 mV to about 20 mV. The TEPD is an active potential representing the transport current of the Na/K pumps in epithelial wall of renal tubules. This study showed that SM electric field can increase TEPD by activation of the pump molecules. Considering renal tubules, many active transporters are driven by the Na(+) concentration gradient built by the Na(+)/K(+) pumps, activation of the pump functions and increase in the magnitude of TEPD imply that the SM electric field may improve reabsorption functions of the renal tubules.

  17. Students’ reasoning when tackling electric field and potential in explanation of dc resistive circuits

    Directory of Open Access Journals (Sweden)

    Ane Leniz

    2017-05-01

    Full Text Available This study examines the causal reasoning that university students use to explain how dc circuits work. We analyze how students use the concepts of electric field and potential difference in their explanatory models of dc circuits, and what kinds of reasoning they use at the macroscopic and microscopic levels in their explanations. This knowledge is essential to help instructors design and implement new teaching approaches that encourage students to articulate the macroscopic and microscopic levels of description. A questionnaire with an emphasis on explanations was used to analyze students’ reasoning. In this analysis of students’ reasoning in the microscopic and macroscopic modeling processes in a dc circuit, we refer to epistemological studies of scientific explanations. We conclude that the student explanations fall into three main categories of reasoning. The vast majority of students employ an explanatory model based on simple or linear causality and on relational reasoning. Moreover, around a third of students use a relational reasoning that relates two magnitudes current and resistance or conductivity of the material, which is included in a macroscopic explanatory model based on Ohm’s law and the conservation of the current. In addition, few students situate the explanations at the microscopic level (charges or electrons with unidirectional cause-effect reasoning. This study looks at a number of aspects that have been little mentioned in previous research at the university level, about the reasoning types students use when establishing macro-micro relationships and some possible difficulties with complex reasoning.

  18. Coarse-Grained Modeling of Nucleic Acids Using Anisotropic Gay-Berne and Electric Multipole Potentials.

    Science.gov (United States)

    Li, Guohui; Shen, Hujun; Zhang, Dinglin; Li, Yan; Wang, Honglei

    2016-02-09

    In this work, we attempt to apply a coarse-grained (CG) model, which is based on anisotropic Gay-Berne and electric multipole (EMP) potentials, to the modeling of nucleic acids. First, a comparison has been made between the CG and atomistic models (AMBER point-charge model) in the modeling of DNA and RNA hairpin structures. The CG results have demonstrated a good quality in maintaining the nucleic acid hairpin structures, in reproducing the dynamics of backbone atoms of nucleic acids, and in describing the hydrogen-bonding interactions between nucleic acid base pairs. Second, the CG and atomistic AMBER models yield comparable results in modeling double-stranded DNA and RNA molecules. It is encouraging that our CG model is capable of reproducing many elastic features of nucleic acid base pairs in terms of the distributions of the interbase pair step parameters (such as shift, slide, tilt, and twist) and the intrabase pair parameters (such as buckle, propeller, shear, and stretch). Finally, The GBEMP model has shown a promising ability to predict the melting temperatures of DNA duplexes with different lengths.

  19. Determination of potential management zones from soil electrical conductivity, yield and crop data.

    Science.gov (United States)

    Li, Yan; Shi, Zhou; Wu, Ci-fang; Li, Hong-yi; Li, Feng

    2008-01-01

    One approach to apply precision agriculture to optimize crop production and environmental quality is identifying management zones. In this paper, the variables of soil electrical conductivity (EC) data, cotton yield data and normalized difference vegetation index (NDVI) data in an about 15 ha field in a coastal saline land were selected as data resources, and their spatial variabilities were firstly analyzed and spatial distribution maps constructed with geostatistics technique. Then fuzzy c-means clustering algorithm was used to define management zones, fuzzy performance index (FPI) and normalized classification entropy (NCE) were used to determine the optimal cluster numbers. Finally one-way variance analysis was performed on 224 georeferenced soil and yield sampling points to assess how well the defined management zones reflected the soil properties and productivity level. The results reveal that the optimal number of management zones for the present study area was 3 and the defined management zones provided a better description of soil properties and yield variation. Statistical analyses indicate significant differences between the chemical properties of soil samples and crop yield in each management zone, and management zone 3 presented the highest nutrient level and potential crop productivity, whereas management zone 1 the lowest. Based on these findings, we conclude that fuzzy c-means clustering approach can be used to delineate management zones by using the given three variables in the coastal saline soils, and the defined management zones form an objective basis for targeting soil samples for nutrient analysis and development of site-specific application strategies.

  20. Simulation of electric potentials and ion motion in planar electrode structures for lossless ion manipulations (SLIM).

    Science.gov (United States)

    Garimella, Sandilya V B; Ibrahim, Yehia M; Webb, Ian K; Tolmachev, Aleksey V; Zhang, Xinyu; Prost, Spencer A; Anderson, Gordon A; Smith, Richard D

    2014-11-01

    We report a conceptual study and computational evaluation of novel planar electrode structures for lossless ion manipulations (SLIM). Planar electrode SLIM components were designed that allow for flexible ion confinement, transport, and storage using a combination of radio frequency (rf) and DC fields. Effective potentials can be generated that provide near ideal regions for confining and manipulating ions in the presence of a gas. Ion trajectory simulations using SIMION 8.1 demonstrated the capability for lossless ion motion in these devices over a wide m/z range and a range of electric fields at low pressures (e.g., a few Torr). More complex ion manipulations (e.g., turning ions by 90(o) and dynamically switching selected ion species into orthogonal channels) are also shown feasible. The performance of SLIM devices at ~4 Torr pressure for performing ion mobility-based separations (IMS) is computationally evaluated and compared with initial experimental results, and both are also shown to agree closely with experimental and theoretical IMS performance for a conventional drift tube design.

  1. Monitoring the Effects of Acupoint Antioxidant Intervention by Measuring Electrical Potential Difference along the Meridian.

    Science.gov (United States)

    Xu, Ming-Ming; Guo, Jing-Ke; Xu, Jin-Sen; Zhang, Chao-Xin; Liu, Shu-Tao; Liao, Ri-Tao; Lin, Chun-Tong; Guo, Jian-Hui; Rao, Ping-Fan

    2015-01-01

    Previous studies suggest that superoxide anions are possibly traveling along acupuncture meridians. The electrical potential difference (EPD) between acupoints may be related to the movement. To test the above hypothesis, we conducted a study investigating the effects of acupoint antioxidant interventions on the meridian EPD. Firstly, ST39 (L) and ST44 (L) were screened out for the EPD detection along the stomach meridian, and ST36 (L) was selected for interventions including acumassage with the control cream, as well as the TAT-SOD cream for 30 minutes, or injection with reduced glutathione sodium. The EPD between ST39 and ST44 was recorded for 80 minutes and measured again 48 h later. While the EPD increased during the acumassage, the acumassage with TAT-SOD cream and the glutathione injection generated waves of EPD increased, indicating the migration or removal from the visceral organ of a greater quantity of superoxide. Remarkably lower EPD readings 48 h later with both antioxidant acupoint interventions than the mere acumassage imply a more complete superoxide flushing out due to the restored superoxide pathway at the acupoint after interventions. The results confirm superoxide transportation along the meridians and demonstrate a possibility of acupoint EPD measurement as a tool to monitor changes in the meridians and acupoints.

  2. Monitoring the Effects of Acupoint Antioxidant Intervention by Measuring Electrical Potential Difference along the Meridian

    Directory of Open Access Journals (Sweden)

    Ming-Ming Xu

    2015-01-01

    Full Text Available Previous studies suggest that superoxide anions are possibly traveling along acupuncture meridians. The electrical potential difference (EPD between acupoints may be related to the movement. To test the above hypothesis, we conducted a study investigating the effects of acupoint antioxidant interventions on the meridian EPD. Firstly, ST39 (L and ST44 (L were screened out for the EPD detection along the stomach meridian, and ST36 (L was selected for interventions including acumassage with the control cream, as well as the TAT-SOD cream for 30 minutes, or injection with reduced glutathione sodium. The EPD between ST39 and ST44 was recorded for 80 minutes and measured again 48 h later. While the EPD increased during the acumassage, the acumassage with TAT-SOD cream and the glutathione injection generated waves of EPD increased, indicating the migration or removal from the visceral organ of a greater quantity of superoxide. Remarkably lower EPD readings 48 h later with both antioxidant acupoint interventions than the mere acumassage imply a more complete superoxide flushing out due to the restored superoxide pathway at the acupoint after interventions. The results confirm superoxide transportation along the meridians and demonstrate a possibility of acupoint EPD measurement as a tool to monitor changes in the meridians and acupoints.

  3. Potential and Limitations of an Electrical Low Pressure Impactor in Disjunct Eddy Covariance Aerosol Flux Measurements

    Science.gov (United States)

    Held, A.; Niessner, R.; Bosveld, F.; Klemm, O.

    2006-12-01

    A micrometeorological application of an electrical low pressure impactor (ELPI) is proposed for the measurement of size-resolved particle fluxes between the surface and the atmosphere. This extends flux measurement capabilities to cover submicron particles in several size classes simultaneously. The disjunct eddy covariance system combines a sonic anemometer, an ELPI and a valve-controlled particle sampling unit. Depending on the valve setting, ambient air or filtered particle-free air is sampled and introduced into the impactor. For disjunct eddy covariance measurements, ambient air is sampled only during a very short sampling interval (~ 0.1 s) in measurement intervals of several seconds. The integrated ELPI signal is representative for the particle size distribution during the short sampling interval. This information may be correlated with fast measurements of the vertical wind speed to obtain turbulent fluxes of submicron particles simultaneously in several size classes. A prototype system has been tested in lab and field experiments in order to evaluate the technical limitations of this approach. Tests show that different valve switch cycles do not affect the concentration measurements. However, longitudinal diffusion processes within the sampling lines lead to signal dilatation. In addition, reliable measurements require a minimum number of charged particles impacting on each stage. This leads to the exclusion of some size bins in typical field applications. Nevertheless, the system showed its potential for size-resolved flux measurements under favorable conditions. Travel support by the ACCENT Access to Infrastructures program is gratefully acknowledged.

  4. Direct conversion of fission energy into electricity in liquid gallium contact potential cell

    Energy Technology Data Exchange (ETDEWEB)

    Soh, T., E-mail: tes211@mail.usask.ca [Univ. of Saskatchewan, Dept. of Mechanical Engineering, Saskatoon, Saskatchewan (Canada); Sohtech R& D Inc., Saskatoon, Saskatchewan (Canada)

    2014-07-01

    Nuclear fission of uranium releases about 93% of its energy in the form of highly charged (up to 20+) and highly energetic fission fragment (83.5MeV average) and other ionizing radiations, including beta and gamma radiations. Liquid gallium is a semimetal that had been previously explored as a self recovering ionization media for alphavoltiac contact potential cell (CPC), and had been evaluated as a suitable replacement coolant for the next generation of fast reactor. By improving the neutronic aspect of a Liquid Gallium CPC (LGaCPC) with low neutron absorption construction materials, and by using a heterogeneous mixture of CANDU fuel grade uranium oxide powder (provided by CAMECO Inc.) and liquid gallium as its junction material, the direct conversion of fission energy to electricity has been observed when irradiated by the thermal neutron flux of the SLOWPOKE-II Research Reactor at the Saskatchewan Research Council. To further explore the effect of temperature on the operation of the LGaCPC, a High Temperature LGaCPC (HTLGaCPC), and a 6 meter high monolithic Large Volume Submersible Neutron Irradiation Chamber (LVSNIC) have been designed and constructed, which allow high temperature fission experiment up to 623K with a thermal neutron flux of 1.18 x 10{sup 10} n/cm{sup 2}/s and the result is presented here, along with discussions on the operating principle of the LGaCPC, and on the construction and measurement techniques used in this study. (author)

  5. Attractivity and acceptance of electric vehicles. Work package 1 of the project OPTUM. Optimization of the potentials of environmental benefits of electric vehicles; Attraktivitaet und Akzeptanz von Elektroautos. Arbeitspaket 1 des Projekts OPTUM. Optimierung der Umweltentlastungspotenziale von Elektrofahrzeugen

    Energy Technology Data Exchange (ETDEWEB)

    Goetz, Konrad; Sunderer, Georg; Birzle-Harder, Barbara; Deffner, Jutta

    2011-10-15

    Germany wants to reduce the greenhouse gas emissions by 40% up to 2020 compared to 1990. Among other reasons, the Federal Government promotes the electric mobility. At least one million electric vehicles shall be brought to the streets up to 2020. As part of the research project OPTUM, an integrative approach for the balancing of the potential environmental benefits of electric vehicles was utilized.

  6. Ways of signal transmission and physiological role of electrical potentials in plants

    Directory of Open Access Journals (Sweden)

    Halina Dziubińska

    2011-01-01

    Full Text Available Plants are subject to stimuli from the environment on which they strongly depend and in contrast to animals, they are unable to escape harmful influences. Therefore, being able to receive stimuli they have developed adequate responses to them. Such a reaction can occur in the area of a stimulus action or cover the whole plant or its parts. In the latter case, it is a systemic reaction. The plant reaction is expressed by various intensity, rate and kind of response. It is interesting to know the character of the signal informing about a stimulus, the routes of its propagation and the transmission mechanism. Three conceptions of excitation are distinguished: 1 propagation of chemical agents formed at the site of a stimulus action with the flow of the phloem sap or through the atmosphere (in the case of volatile substances to other plant parts, 2 a very fast transmission by the xylem in the wave of hydraulic pressure formed after a plant damage. From combining the "hydraulic" and "chemical" hypothesis a conception of hydraulic dispersion has been formulated which assumes that chemical substances synthetized after an injury can be transferred very fast with the wave of hydraulic pressure changes in the whole plant, 3 a stimulus evokes the action potential (AP, and its transmission along the whole plant, plant organ or specialized tissue, by local circuits from cell to cell. Strong, damaging stimuli can evoke variation potentials (VPs, the character of which differs from APs. It is postulated that transmission of VP occurs by a hydraulic dispersion and electrical changes seem to be secondary phenomena.

  7. Structures, phase stabilities, and electrical potentials of Li-Si battery anode materials

    KAUST Repository

    Tipton, William W.

    2013-05-28

    The Li-Si materials system holds promise for use as an anode in Li-ion battery applications. For this system, we determine the charge capacity, voltage profiles, and energy storage density solely by ab initio methods without any experimental input. We determine the energetics of the stable and metastable Li-Si phases likely to form during the charging and discharging of a battery. Ab initio molecular dynamics simulations are used to model the structure of amorphous Li-Si as a function of composition, and a genetic algorithm coupled to density-functional theory searches the Li-Si binary phase diagram for small-cell, metastable crystal structures. Calculations of the phonon densities of states using density-functional perturbation theory for selected structures determine the importance of vibrational, including zero-point, contributions to the free energies. The energetics and local structural motifs of these metastable Li-Si phases closely resemble those of the amorphous phases, making these small unit cell crystal phases good approximants of the amorphous phase for use in further studies. The charge capacity is estimated, and the electrical potential profiles and the energy density of Li-Si anodes are predicted. We find, in good agreement with experimental measurements, that the formation of amorphous Li-Si only slightly increases the anode potential. Additionally, the genetic algorithm identifies a previously unreported member of the Li-Si binary phase diagram with composition Li5Si2 which is stable at 0 K with respect to previously known phases. We discuss its relationship to the partially occupied Li7Si3 phase. © 2013 American Physical Society.

  8. Potential Effect and Analysis of High Residential Solar Photovoltaic (PV Systems Penetration to an Electric Distribution Utility (DU

    Directory of Open Access Journals (Sweden)

    Jeffrey Tamba Dellosa

    2016-11-01

    Full Text Available The Renewable Energy Act of 2008 in the Philippines provided an impetus for residential owners to explore solar PV installations at their own rooftops through the Net-Metering policy. The Net-Metering implementation through the law however presented some concerns with inexperienced electric DU on the potential effect of high residential solar PV system installations. It was not known how a high degree of solar integration to the grid can possibly affect the operations of the electric DU in terms of energy load management. The primary objective of this study was to help the local electric DU in the analysis of the potential effect of high residential solar PV system penetration to the supply and demand load profile in an electric distribution utility (DU grid in the province of Agusan del Norte, Philippines. The energy consumption profiles in the year 2015 were obtained from the electric DU operating in the area. An average daily energy demand load profile was obtained from 0-hr to the 24th hour of the day based from the figures provided by the electric DU. The assessment part of the potential effect of high solar PV system integration assumed four potential total capacities from 10 Mega Watts (MW to 40 MW generated by all subscribers in the area under study at a 10 MW interval. The effect of these capacities were measured and analyzed with respect to the average daily load profile of the DU. Results of this study showed that a combined installations beyond 20 MWp coming from all subscribers is not viable for the local electric DU based on their current energy demand or load profile. Based from the results obtained, the electric DU can make better decisions in the management of high capacity penetration of solar PV systems in the future, including investment in storage systems when extra capacities are generated. Article History: Received July 15th 2016; Received in revised form Sept 23rd 2016; Accepted Oct 1st 2016; Available online How to Cite

  9. On micro to mesoscale homogenization of electrical properties for damaged laminated composites (and their potential applications in electrical tomography)

    KAUST Repository

    Selvakumaran, Lakshmi

    2015-12-01

    Efficient and optimal use of composites in structures requires tools to monitor and capture the complex degradation that can occur within the laminates over time. Structural health monitoring (SHM) techniques uses sensors/actuators on the structure to progressively monitor the health of the structure with minimal manual intervention. Electrical tomography (ET) is a SHM technique that uses voltage measurements from the surface of the laminate to reconstruct a conductivity map of the structure. Since damage has been shown to modify the conductivity of the laminate, the conductivity map can provide an indirect measure of the damage within the material. Studies have shown the capability of ET to identify macroscale damage due to impact. But, little has been done to quantitatively assess damage using ET. In this work, we present a theoretical framework to link degradation mechanisms occuring at the microscale to the conductivity at the mesoscale through damage indicators. The mesoscale damage indicators are then shown to be intrinsic to the ply. Next, we use the knowledge obtained through mesoscale homogenization to study the detectability of transverse cracks. Last, we show how the mesoscale homogenization participates in regularization of the inverse problem and in the quantitative assessment of the reconstructed conductivity map. This is as such the first step towards turning ET into a viable quantitative health monitoring technique.

  10. Towards Improving Resilience of Smart Urban Electricity Networks by Interactively Assessing Potential Microgrids

    NARCIS (Netherlands)

    Tseng Lau, Eng; Keong Chai, Kok; Chen, Yue; Vasenev, Alexandr

    2017-01-01

    When a city adds a renewable generation to improve its carbon footprint, this step towards a greener city can be a step towards a smarter city. Strategical positioning of new urban electricity components makes the city more resilient to electricity outages. Money and resilience are two conflicting

  11. The potential role of waste biomass in the future urban electricity system

    NARCIS (Netherlands)

    Jiang, Yu; Werf, van der Edwin; Ierland, van Ekko C.; Keesman, Karel J.

    2017-01-01

    The share of intermittent renewable electricity (IRE) in the future urban electricity system is expected to increase significantly. Sufficient back-up capacity is needed in the period when IRE output is low. Bioenergy is both dispatchable and carbon-neutral, and can hence be a promising option to

  12. Targeting Host Cell Surface Nucleolin for RSV Therapy: Challenges and Opportunities

    Directory of Open Access Journals (Sweden)

    Peter Mastrangelo

    2017-09-01

    Full Text Available Nucleolin (NCL has been reported as a cellular receptor for the human respiratory syncytial virus (RSV. We studied the effects of re-purposing AS1411, an anti-cancer compound that binds cell surface NCL, as a possible novel strategy for RSV therapy in vitro and in vivo. AS1411 was administered to RSV-infected cultures of non-polarized (HEp-2 and polarized (MDCK epithelial cells and to virus-infected mice and cotton rats. Results of in vitro experiments showed that AS1411, used in micromolar concentrations, was associated with decreases in the number of virus-positive cells. Intranasal administration of AS1411 (50 mg/kg to RSV-infected mice and cotton rats was associated with partial reductions in lung viral titers, decreased virus-associated airway inflammation, and decreased IL-4/IFN-γ ratios when compared to untreated, infected animals. In conclusion, our findings indicate that therapeutic use of AS1411 has modest effects on RSV replication and host response. While the results underscore the challenges of targeting cell surface NCL as a potential novel strategy for RSV therapy, they also highlight the potential of cell surface NCL as a therapeutic target.

  13. Targeting Host Cell Surface Nucleolin for RSV Therapy: Challenges and Opportunities.

    Science.gov (United States)

    Mastrangelo, Peter; Norris, Michael J; Duan, Wenming; Barrett, Edward G; Moraes, Theo J; Hegele, Richard G

    2017-09-19

    Nucleolin (NCL) has been reported as a cellular receptor for the human respiratory syncytial virus (RSV). We studied the effects of re-purposing AS1411, an anti-cancer compound that binds cell surface NCL, as a possible novel strategy for RSV therapy in vitro and in vivo. AS1411 was administered to RSV-infected cultures of non-polarized (HEp-2) and polarized (MDCK) epithelial cells and to virus-infected mice and cotton rats. Results of in vitro experiments showed that AS1411, used in micromolar concentrations, was associated with decreases in the number of virus-positive cells. Intranasal administration of AS1411 (50 mg/kg) to RSV-infected mice and cotton rats was associated with partial reductions in lung viral titers, decreased virus-associated airway inflammation, and decreased IL-4/IFN-γ ratios when compared to untreated, infected animals. In conclusion, our findings indicate that therapeutic use of AS1411 has modest effects on RSV replication and host response. While the results underscore the challenges of targeting cell surface NCL as a potential novel strategy for RSV therapy, they also highlight the potential of cell surface NCL as a therapeutic target.

  14. Transthoracic defibrillation potential gradients in a closed chest porcine model of prolonged spontaneous and electrically induced ventricular fibrillation.

    Science.gov (United States)

    Niemann, James T; Rosborough, John P; Youngquist, Scott T; Shah, Atman P

    2010-04-01

    The purpose of this study was to measure the local electrical field or potential gradient, measured with a catheter-based system, required to terminate long duration electrically or ischaemically induced ventricular fibrillation (VF). We hypothesized that prolonged ischaemic VF would be more difficult to terminate when compared to electrically induced VF of similar duration. Thirty anesthetized and instrumented swine were randomized to electrically induced VF or spontaneous, ischaemically induced VF, produced by balloon occlusion of the left anterior descending coronary artery. After 7 min of VF, chest compressions were initiated and rescue shocks were attempted 1 min later. The potential gradient for each shock was measured and the mean values required for defibrillation compared for the VF groups. The number of shocks and the shock strength required for termination of VF were not significantly different for the groups. The potential gradient of the first successful defibrillating shock was significantly greater in the spontaneous, occlusion-induced VF group (12.80+/-2.82 V/cm vs 9.60+/-2.48 V/cm, p=0.002). The number of refibrillations was greater in the ischaemic group than in the non-ischaemic electrical group (6+/-4 vs 1+/-1, pDefibrillation of prolonged VF produced by acute myocardial ischaemia requires a significantly greater potential gradient to terminate than prolonged VF induced by electrical stimulation of the right ventricular endocardium. The VF duration used in this study approximates that occurring in victims of out-of-hospital cardiac arrest. Our findings may be of clinical importance in the management of such patients. Copyright (c) 2010 Elsevier Ireland Ltd. All rights reserved.

  15. Transthoracic Defibrillation Potential Gradients in a Closed Chest Porcine Model of Prolonged Spontaneous and Electrically Induced Ventricular Fibrillation

    Science.gov (United States)

    Niemann, James T.; Rosborough, John P.; Youngquist, Scott T.; Shah, Atman P.

    2010-01-01

    Summary Objective The purpose of this study was to measure the local electrical field or potential gradient, measured with a catheter-based system, required to terminate long duration electrically or ischaemically induced ventricular fibrillation (VF). We hypothesized that prolonged ischaemic VF would be more difficult to terminate when compared to electrically induced VF of similar duration. Methods Thirty anesthetized and instrumented swine were randomized to electrically induced VF or spontaneous, ischemically induced VF, produced by balloon occlusion of the left anterior descending coronary artery. After 7 min of VF, chest compressions were initiated and rescue shocks were attempted 1 min later. The potential gradient for each shock was measured and the mean values required for defibrillation compared for the VF all. Results The number of shocks and the shock strength required for termination of VF were not significantly different for the all. The potential gradient of the first successful defibrillating shock was significantly greater in the spontaneous, occlusion induced VF group (12.80 ± 2.82 vs 9.60 ± 2.48 V/cm, p = 0.002). The number of refibrillations was greater in the ischaemic group than in the non-ischaemic electrical group (6 ± 4 versus 1 ± 1, p Defibrillation of prolonged VF produced by acute myocardial ischaemia requires a significantly greater potential gradient to terminate than prolonged VF induced by electrical stimulation of the right ventricular endocardium. The VF duration used in this study approximates that occurring in victims of out-of-hospital cardiac arrest. Our findings may be of clinical importance in the management of such patients. PMID:20122785

  16. Transcranial electrical motor-evoked potential monitoring during surgery for spinal deformity - A study of 145 patients

    NARCIS (Netherlands)

    Langeloo, DD; Lelivelt, A; Journee, HL; Slappendel, R; de Kleuver, M

    2003-01-01

    Study Design. A descriptive historic cohort study was conducted. Objectives. To determine intraoperative response amplitude criteria for transcranial electrical motor-evoked potential monitoring that warn of neurologic damage, and to determine the additional value of monitoring six instead of two

  17. The Confidence-Accuracy Relationship in Diagnostic Assessment: The Case of the Potential Difference in Parallel Electric Circuits

    Science.gov (United States)

    Saglam, Murat

    2015-01-01

    This study explored the relationship between accuracy of and confidence in performance of 114 prospective primary school teachers in answering diagnostic questions on potential difference in parallel electric circuits. The participants were required to indicate their confidence in their answers for each question. Bias and calibration indices were…

  18. Modeling the electric potential across neuronal membranes: the effect of fixed charges on spinal ganglion neurons and neuroblastoma cells.

    Directory of Open Access Journals (Sweden)

    Thiago M Pinto

    Full Text Available We present a model for the electric potential profile across the membranes of neuronal cells. We considered the resting and action potential states, and analyzed the influence of fixed charges of the membrane on its electric potential, based on experimental values of membrane properties of the spinal ganglion neuron and the neuroblastoma cell. The spinal ganglion neuron represents a healthy neuron, and the neuroblastoma cell, which is tumorous, represents a pathological neuron. We numerically solved the non-linear Poisson-Boltzmann equation for the regions of the membrane model we have adopted, by considering the densities of charges dissolved in an electrolytic solution and fixed on both glycocalyx and cytoplasmic proteins. Our model predicts that there is a difference in the behavior of the electric potential profiles of the two types of cells, in response to changes in charge concentrations in the membrane. Our results also describe an insensitivity of the neuroblastoma cell membrane, as observed in some biological experiments. This electrical property may be responsible for the low pharmacological response of the neuroblastoma to certain chemotherapeutic treatments.

  19. Analysis of electrically evoked compound action potential of the auditory nerve in children with bilateral cochlear implants.

    Science.gov (United States)

    Caldas, Fernanda Ferreira; Cardoso, Carolina Costa; Barreto, Monique Antunes de Souza Chelminski; Teixeira, Marina Santos; Hilgenberg, Anacléia Melo da Silva; Serra, Lucieny Silva Martins; Bahmad Junior, Fayez

    2016-01-01

    The cochlear implant device has the capacity to measure the electrically evoked compound action potential of the auditory nerve. The neural response telemetry is used in order to measure the electrically evoked compound action potential of the auditory nerve. To analyze the electrically evoked compound action potential, through the neural response telemetry, in children with bilateral cochlear implants. This is an analytical, prospective, longitudinal, historical cohort study. Six children, aged 1-4 years, with bilateral cochlear implant were assessed at five different intervals during their first year of cochlear implant use. There were significant differences in follow-up time (p=0.0082) and electrode position (p=0.0019) in the T-NRT measure. There was a significant difference in the interaction between time of follow-up and electrode position (p=0.0143) when measuring the N1-P1 wave amplitude between the three electrodes at each time of follow-up. The electrically evoked compound action potential measurement using neural response telemetry in children with bilateral cochlear implants during the first year of follow-up was effective in demonstrating the synchronized bilateral development of the peripheral auditory pathways in the studied population. Copyright © 2015 Associação Brasileira de Otorrinolaringologia e Cirurgia Cérvico-Facial. Published by Elsevier Editora Ltda. All rights reserved.

  20. Asymptotic behavior of correlation functions for electric potential and field fluctuations in a classical one-component plasma

    NARCIS (Netherlands)

    Suttorp, L.G.

    1992-01-01

    The correlations of the electric potential fluctuations in a classical one-component plasma are studied for large distances between the observation points. The two-point correlation function for these fluctuations is known to decay slowly for large distances, even if exponential clustering holds for

  1. Criteria for transcranial electrical motor evoked potential monitoring during spinal deformity surgery - A review and discussion of the literature

    NARCIS (Netherlands)

    Langeloo, D. -D.; Journee, H. -L.; de Kleuver, M.; Grotenhuis, J. A.

    2007-01-01

    Transcranial electrical stimulated motor evoked potential monitoring (TES-MEP) has proven to be a successful and reliable neuromonitoring technique during spinal correction surgery. However, three criteria for TES-MEP monitoring have been described in the literature. This study aims at discussing

  2. Elevated cell-surface hyaluronate in substrate-attached cells

    Energy Technology Data Exchange (ETDEWEB)

    Kraemer, P.M.; Barnhart, B.J.

    1978-01-01

    CHO cells, an anchorage-independent Chinese hamster cell line, synthesize and deposit more hyaluronic acid into the cell-surface material when attached to substrate than when growing in suspension. The difference cannot be explained by differences in turnover, cellular localization, or secretion. Evidently the anchorage state per se stimulates hyaluronic acid synthesis.

  3. Regulation of tissue factor coagulant activity on cell surfaces.

    Science.gov (United States)

    Rao, L V M; Pendurthi, U R

    2012-11-01

    Tissue factor (TF) is a transmembrane glycoprotein and an essential component of the factor VIIa-TF enzymatic complex that triggers activation of the coagulation cascade. Formation of TF-FVIIa complexes on cell surfaces not only trigger the coagulation cascade but also transduce cell signaling via activation of protease-activated receptors. Tissue factor is expressed constitutively on cell surfaces of a variety of extravascular cell types, including fibroblasts and pericytes in and surrounding blood vessel walls and epithelial cells, but is generally absent on cells that come into contact with blood directly. However, TF expression could be induced in some blood cells, such as monocytes and endothelial cells, following an injury or pathological stimuli. Tissue factor is essential for hemostasis, but aberrant expression of TF leads to thrombosis. Therefore, a proper regulation of TF activity is critical for the maintenance of hemostatic balance and health in general. TF-FVIIa coagulant activity at the cell surface is influenced not only by TF protein expression levels but also independently by a variety of mechanisms, including alterations in membrane phospholipid composition and cholesterol content, thiol-dependent modifications of TF allosteric disulfide bonds, and other post-translational modifications of TF. In this article, we critically review the key literature on mechanisms by which TF coagulant activity is regulated at the cell surface in the absence of changes in TF protein levels with specific emphasis on recently published data and provide the authors' perspective on the subject. © 2012 International Society on Thrombosis and Haemostasis.

  4. An update on cell surface proteins containing extensin-motifs.

    Science.gov (United States)

    Borassi, Cecilia; Sede, Ana R; Mecchia, Martin A; Salgado Salter, Juan D; Marzol, Eliana; Muschietti, Jorge P; Estevez, Jose M

    2016-01-01

    In recent years it has become clear that there are several molecular links that interconnect the plant cell surface continuum, which is highly important in many biological processes such as plant growth, development, and interaction with the environment. The plant cell surface continuum can be defined as the space that contains and interlinks the cell wall, plasma membrane and cytoskeleton compartments. In this review, we provide an updated view of cell surface proteins that include modular domains with an extensin (EXT)-motif followed by a cytoplasmic kinase-like domain, known as PERKs (for proline-rich extensin-like receptor kinases); with an EXT-motif and an actin binding domain, known as formins; and with extracellular hybrid-EXTs. We focus our attention on the EXT-motifs with the short sequence Ser-Pro(3-5), which is found in several different protein contexts within the same extracellular space, highlighting a putative conserved structural and functional role. A closer understanding of the dynamic regulation of plant cell surface continuum and its relationship with the downstream signalling cascade is a crucial forthcoming challenge. © The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.

  5. Analysis of Potential Energy Corridors Proposed by the Western Electricity Coordinating Council

    Energy Technology Data Exchange (ETDEWEB)

    Kuiper, James A.; Cantwell, Brian J.; Hlava, Kevin J.; Moore, H Robert; Orr, Andrew B.; Zvolanek, Emily A.

    2014-02-24

    This report, Analysis of Potential Energy Corridors Proposed by the Western Electricity Coordinating Council (WECC), was prepared by the Environmental Science Division of Argonne National Laboratory (Argonne). The intent of WECC’s work was to identify planning-level energy corridors that the Department of Energy (DOE) and its affiliates could study in greater detail. Argonne was tasked by DOE to analyze the WECC Proposed Energy Corridors in five topic areas for use in reviewing and revising existing corridors, as well as designating additional energy corridors in the 11 western states. In compliance with Section 368 of the Energy Policy Act of 2005 (EPAct), the Secretaries of Energy, Agriculture, and the Interior (Secretaries) published a Programmatic Environmental Impact Statement in 2008 to address the proposed designation of energy transport corridors on federal lands in the 11 western states. Subsequently, Records of Decision designating the corridors were issued in 2009 by the Bureau of Land Management (BLM) and the U.S. Forest Service (USFS). The 2012 settlement of a lawsuit, brought by The Wilderness Society and others against the United States, which identified environmental concerns for many of the corridors requires, among other things, periodic reviews of the corridors to assess the need for revisions, deletions, or additions. A 2013 Presidential Memorandum requires the Secretaries to undertake a continuing effort to identify and designate energy corridors. The WECC Proposed Energy Corridors and their analyses in this report provide key information for reviewing and revising existing corridors, as well as designating additional energy corridors in the 11 western states. Load centers and generation hubs identified in the WECC analysis, particularly as they reflect renewable energy development, would be useful in reviewing and potentially updating the designated Section 368 corridor network. Argonne used Geographic Information System (GIS) technology to

  6. Similar simulation study on the characteristics of the electric potential response to coal mining

    Science.gov (United States)

    Niu, Yue; Li, Zhonghui; Kong, Biao; Wang, Enyuan; Lou, Quan; Qiu, Liming; Kong, Xiangguo; Wang, Jiali; Dong, Mingfu; Li, Baolin

    2018-02-01

    An electric potential (EP) can be generated during the failure process of coal and rock. In this article, a similar physical model of coal rock was built and the characteristics of the EP responding to the process of coal mining were studied. The results showed that, at the early mining stage, the structure of coal rock strata were stable in the simulation model, the support stress of overlying coal rock strata was low and the maximum subsidence was little, while the EP change was less. With the advancement of the working face, the support stress of the overlying coal rock strata in the mined-out area changed dramatically, the maximum subsidence increased constantly, the deformation and destruction were aggravated, and cracks expanded continuously. Meanwhile, the EP response was significant with fluctuation. When significant macro damage appeared in coal rock strata, the EP signal fluctuation was violent. The overlying coal rock strata were influenced by gravity and mining activity. During the mining process, the crack growth and the friction, together with slip between coal and rock particles, resulted in the response of EP. The change in EP was closely related to the damage state and stress distribution of the coal rock strata. EP monitoring has the advantages of accurate reflection and strong anti-interference in the field. Therefore, with further study, an EP monitoring method could be applied for monitoring and early warning of coal and rock dynamic disaster, and risk evaluation in the future. The strength of the EP and its fluctuation degree could serve as the key discrimination indexes.

  7. Wind-Electric Power Potential Assessment for Three Locations in East Java-Indonesia

    Directory of Open Access Journals (Sweden)

    Ali Musyafa

    2011-08-01

    Full Text Available This paper reports our effort to asses wind energy potentials for three locations in East Java. We used wind speed data over a period of almost 3 years, i.e. in period of June 2006 – August 2008. Data were taken from direct measurement in locations in East Java Province, i.e. Sampang (Madura, Juanda (Surabaya, and Sawahan (Nganjuk. The short-term of wind speed mean in monthly signifies to wind-speed value ”which parallels to the wind turbine power curve value” were used to estimate the annual energy output for a 1 MW installed capacity wind farm on the each site 100 of 10kW rated wind turbines were used in the analysis. The short term of wind speed mean at Surabaya and Nganjuk were 2.34, 3.03 and 1.97 m/s at 2 m Above Ground Level (AGL, respectively. In both locations, wind speeds were observed during the day time between 04.00 and 18.00 and relatively smaller ones between 19.00 and 03.00 period. Meanwhile, in Sampang (Madura the higher wind speeds were observed between 20.00 and 06.00, and relatively smaller between 07.00 and 19.00 period. The 1 MW windfarm at Sampang, Surabaya and Nganjuk can produce 1.284; 1.199 and 1.008 MWh of electricity yearly, taking into consideration of the temperature adjustment coefficien of about 6 %. The plant capacity factor at Sampang, Surabaya and Nganjuk were found to be 30.02 %, 30.00 % and 30.01 % respectively. Additionally, it is noticed that these site can contribute to the avoidance of 0.904; 0.846 and 0.709 tons/year of CO2 equivalent Green House Gases (GHG from entering into the local atmosphere, thus creating a clean and healthy athmosphere for local inhabitants.

  8. Determination of potential management zones from soil electrical conductivity, yield and crop data*

    Science.gov (United States)

    Li, Yan; Shi, Zhou; Wu, Ci-fang; Li, Hong-yi; Li, Feng

    2008-01-01

    One approach to apply precision agriculture to optimize crop production and environmental quality is identifying management zones. In this paper, the variables of soil electrical conductivity (EC) data, cotton yield data and normalized difference vegetation index (NDVI) data in an about 15 ha field in a coastal saline land were selected as data resources, and their spatial variabilities were firstly analyzed and spatial distribution maps constructed with geostatistics technique. Then fuzzy c-means clustering algorithm was used to define management zones, fuzzy performance index (FPI) and normalized classification entropy (NCE) were used to determine the optimal cluster numbers. Finally one-way variance analysis was performed on 224 georeferenced soil and yield sampling points to assess how well the defined management zones reflected the soil properties and productivity level. The results reveal that the optimal number of management zones for the present study area was 3 and the defined management zones provided a better description of soil properties and yield variation. Statistical analyses indicate significant differences between the chemical properties of soil samples and crop yield in each management zone, and management zone 3 presented the highest nutrient level and potential crop productivity, whereas management zone 1 the lowest. Based on these findings, we conclude that fuzzy c-means clustering approach can be used to delineate management zones by using the given three variables in the coastal saline soils, and the defined management zones form an objective basis for targeting soil samples for nutrient analysis and development of site-specific application strategies. PMID:18196615

  9. Electrically evoked compound action potentials artefact rejection by independent component analysis: procedure automation.

    Science.gov (United States)

    Akhoun, Idrick; McKay, Colette; El-Deredy, Wael

    2015-01-15

    Independent-components-analysis (ICA) successfully separated electrically-evoked compound action potentials (ECAPs) from the stimulation artefact and noise (ECAP-ICA, Akhoun et al., 2013). This paper shows how to automate the ECAP-ICA artefact cancellation process. Raw-ECAPs without artefact rejection were consecutively recorded for each stimulation condition from at least 8 intra-cochlear electrodes. Firstly, amplifier-saturated recordings were discarded, and the data from different stimulus conditions (different current-levels) were concatenated temporally. The key aspect of the automation procedure was the sequential deductive source categorisation after ICA was applied with a restriction to 4 sources. The stereotypical aspect of the 4 sources enables their automatic classification as two artefact components, a noise and the sought ECAP based on theoretical and empirical considerations. The automatic procedure was tested using 8 cochlear implant (CI) users and one to four stimulus electrodes. The artefact and noise sources were successively identified and discarded, leaving the ECAP as the remaining source. The automated ECAP-ICA procedure successfully extracted the correct ECAPs compared to standard clinical forward masking paradigm in 22 out of 26 cases. ECAP-ICA does not require extracting the ECAP from a combination of distinct buffers as it is the case with regular methods. It is an alternative that does not have the possible bias of traditional artefact rejections such as alternate-polarity or forward-masking paradigms. The ECAP-ICA procedure bears clinical relevance, for example as the artefact rejection sub-module of automated ECAP-threshold detection techniques, which are common features of CI clinical fitting software. Copyright © 2014. Published by Elsevier B.V.

  10. Electrically switchable polymer stabilised broadband infrared reflectors and their potential as smart windows for energy saving in buildings.

    Science.gov (United States)

    Khandelwal, Hitesh; Loonen, Roel C G M; Hensen, Jan L M; Debije, Michael G; Schenning, Albertus P H J

    2015-07-01

    Electrically switchable broadband infrared reflectors that are relatively transparent in the visible region have been fabricated using polymer stabilised cholesteric liquid crystals. The IR reflectors can change their reflection/transmission properties by applying a voltage in response to changes in environmental conditions. Simulations predict that a significant amount of energy can be saved on heating, cooling and lighting of buildings in places such as Madrid by using this switchable IR reflector. We have also fabricated a switchable IR reflector which can also generate electricity. These polymer based switchable IR reflectors are of high potential as windows of automobiles and buildings to control interior temperatures and save energy.

  11. Cell Surface-Associated Proteins in the Filamentous Cyanobacterium Anabaena sp. strain PCC 7120

    OpenAIRE

    Yoshimura, Hidehisa; Ikeuchi, Masahiko; Ohomori, Masayuki

    2012-01-01

    The cell surface senses environmental changes first and transfers signals into the cell. To understand the response to environmental changes, it is necessary to analyze cell surface components, particularly cell surface-associated proteins. We therefore investigated cell surface-associated proteins from the filamentous cyanobacterium Anabaena sp. strain PCC 7120. The cell surface-associated proteins extracted by an acidic buffer were resolved by SDS-PAGE. Eighteen proteins were identified fro...

  12. Potential For Plug-In Electric Vehicles To Provide Grid Support Services

    Energy Technology Data Exchange (ETDEWEB)

    Dias, F. G.; Luo, Y.; Mohanpurkar, M.; Hovsapian, R.; Scoffield, D.

    2017-04-01

    Since the modern-day introduction of plug-in electric vehicles (PEVs), scientists have proposed leveraging PEV battery packs as distributed energy resources for the electric grid. PEV charging can be controlled not only to provide energy for transportation but also to provide grid services and to facilitate the integration of renewable energy generation. With renewable generation increasing at an unprecedented rate, most of which is non-dispatchable and intermittent, the concept of using PEVs as controllable loads is appealing to electric utilities. This additional functionality could also provide value to PEV owners and drive PEV adoption. It has been widely proposed that PEVs can provide valuable grid services, such as load shifting to provide voltage regulation. The objective this work is to address the degree to which PEVs can provide grid services and mutually benefit the electric utilities, PEV owners, and auto manufacturers.

  13. Electrical double layer at various electrode potentials: A modification by vibration

    Czech Academy of Sciences Publication Activity Database

    Zhan, H.; Červenka, Jiří; Prawer, S.; Garrett, D.J.

    2017-01-01

    Roč. 121, č. 8 (2017), s. 4760-4764 ISSN 1932-7447 Institutional support: RVO:68378271 Keywords : electrical double layer * vibration * high concentration * model Subject RIV: CF - Physical ; Theoretical Chemistry Impact factor: 4.536, year: 2016

  14. Direct current electric potential in an anisotropic half-space with vertical contact containing a conductive 3D body

    Directory of Open Access Journals (Sweden)

    Li Ping

    2004-01-01

    Full Text Available Detailed studies of anomalous conductors in otherwise homogeneous media have been modelled. Vertical contacts form common geometries in galvanic studies when describing geological formations with different electrical conductivities on either side. However, previous studies of vertical discontinuities have been mainly concerned with isotropic environments. In this paper, we deal with the effect on the electric potentials, such as mise-à-la-masse anomalies, due to a conductor near a vertical contact between two anisotropic regions. We also demonstrate the interactive effects when the conductive body is placed across the vertical contact. This problem is normally very difficult to solve by the traditional numerical methods. The integral equations for the electric potential in anisotropic half-spaces are established. Green's function is obtained using the reflection and transmission image method in which five images are needed to fit the boundary conditions on the vertical interface and the air-earth surface. The effects of the anisotropy of the environments and the conductive body on the electric potential are illustrated with the aid of several numerical examples.

  15. Electricity consumption and CO{sub 2} capture potential in Spain

    Energy Technology Data Exchange (ETDEWEB)

    Romeo, Luis M.; Calvo, Elena; Valero, Antonio; De Vita, Alessia [Centre of Research for Energy Resources and Consumptions (CIRCE), University of Zaragoza, Maria de Luna, 3-50018 Zaragoza (Spain)

    2009-09-15

    In this paper, different electricity demand scenarios for Spain are presented. Population, income per capita, energy intensity and the contribution of electricity to the total energy demand have been taken into account in the calculations. Technological role of different generation technologies, i.e. coal, nuclear, renewable, combined cycle (CC), combined heat and power (CHP) and carbon capture and storage (CCS), are examined in the form of scenarios up to 2050. Nine future scenarios corresponding to three electrical demands and three options for new capacity: minimum cost of electricity, minimum CO{sub 2} emissions and a criterion with a compromise between CO{sub 2} and cost (CO{sub 2}-cost criterion) have been proposed. Calculations show reduction in CO{sub 2} emissions from 2020 to 2030, reaching a maximum CO{sub 2} emission reduction of 90% in 2050 in an efficiency scenario with CCS and renewables. The contribution of CCS from 2030 is important with percentage values of electricity production around 22-28% in 2050. The cost of electricity (COE) increases up to 25% in 2030, and then this value remains approximately constant or decreases slightly. (author)

  16. A systems model and potential leverage points for base load electric generating options

    Energy Technology Data Exchange (ETDEWEB)

    Brownson, D.A.; Hanson, D.J.; Price, L.G.; Sebo, D.E.

    1993-09-01

    The mission and structure of electric utilities may change significantly to meet the challenges on the next several decades. In addition, providing electrical energy in an environmentally responsible manner will continue to be a major challenge. The methods of supplying electrical power may change dramatically in the future as utilities search for ways to improve the availability and reliability of electrical power systems. The role of large, base load generating capacity to supply the bulk of a utility`s electrical power is evolving, but it will continue to be important for many years to come. The objective of this study is to examine the systems structure of five base load capacity options available to a utility and identify areas where technological improvements could produce significant changes in their systems. These improvements would enhance the likelihood that these options would be selected for providing future electrical capacity. Technology improvements are identified and discussed, but it was beyond the scope of this work to develop strategies for specific Idaho National Engineering Laboratory involvement.

  17. Cost-saving potentials using demand side management in households and electric mobility

    Energy Technology Data Exchange (ETDEWEB)

    Schinz, S.; Najib, H.; Steiner, L. [Darmstadt Univ. of Technology (Germany). Inst. for Renewable Energies

    2010-07-01

    The increasing share of renewable energies in the electricity production has an influence on the grid and on the electricity markets, where higher price fluctuations are expected. One way of integrating renewable energies is using demand side management (DSM). In this case online energy markets enable private households to participate with their loads in these markets. Especially their household appliances participate on the basis of hourly electricity prices. Thus these markets can provide DSM-services for the grid by means of aggregating household loads. To estimate the cost-savings of DSM for the purpose of reducing electricity costs in private households different gadget-models are developed and minimal costs are calculated by formulating linear optimization models. In private households especially refrigerators, freezers, washing machines, tumble- dryers and dishwashers can be considered as shiftable loads for DSM purposes. This application of DSM is examined in this paper precisely. Future developments regarding DSM are analyzed with scenarios for 2010 and 2030. The gadget-models have average specifications of gadgets in Europe and Germany. Their operation is optimized for minimal electricity cost over one year on the basis of electricity prices from the European Energy Exchange EEX in Leipzig, Germany. Finally DSM in Micro Grids is analyzed and different control options are considered to solve the voltage stability problem. (orig.)

  18. Efficient Isolation and Quantitative Proteomic Analysis of Cancer Cell Plasma Membrane Proteins for Identification of Metastasis-Associated Cell Surface Markers

    DEFF Research Database (Denmark)

    Lund, Rikke; Leth-Larsen, Rikke; Jensen, Ole N

    2009-01-01

    Cell surface membrane proteins are involved in central processes such as cell signaling, cell-cell interactions, ion and solute transport, and they seem to play a pivotal role in several steps of the metastatic process of cancer cells. The low abundance and hydrophobic nature of cell surface memb...... peptides of which 622 (300 at SL80) were membrane proteins. The quantitative proteomic analysis identified 16 cell surface proteins as potential markers of the ability of breast cancer cells to form distant metastases....... membrane proteins complicate their purification and identification by MS. We used two isogenic cell lines with opposite metastatic capabilities in nude mice to optimize cell surface membrane protein purification and to identify potential novel markers of metastatic cancer. The cell surface membrane......Cell surface membrane proteins are involved in central processes such as cell signaling, cell-cell interactions, ion and solute transport, and they seem to play a pivotal role in several steps of the metastatic process of cancer cells. The low abundance and hydrophobic nature of cell surface...

  19. Microbial Anchoring Systems for Cell-Surface Display of Lipolytic Enzymes

    Directory of Open Access Journals (Sweden)

    Ana Bielen

    2014-01-01

    Full Text Available Studies of microbial cell envelopes and particularly cell surface proteins and mechanisms of their localization brought about new biotechnological applications of the gained knowledge in surface display of homologous and heterologous proteins. By fusing surface proteins or their anchoring domains with different proteins of interest, their so-called genetic immobilization is achieved. Hybrid proteins are engineered in a way that they are expressed in the host cells, secreted to the cell surface and incorporated into the wall/ envelope moiety. In this way, laborious and often detrimental procedure of chemical immobilization of the protein is avoided by letting the cells do the whole procedure. Both bacterial and yeast cells have been used for this purpose and a number of potential biotechnological applications of surface-displayed proteins have been reported. Among the most frequently used passenger proteins are lipolytic enzymes, due to their great technological significance and numerous important applications. In this review, our current knowledge on mechanisms and molecular systems for surface display of lipolytic enzymes on bacterial and yeast cell surfaces is summarized.

  20. Evaluation of the steric, electric, and dielectric exclusion model on the basis of salt rejection rate and membrane potential measurements.

    Science.gov (United States)

    Lanteri, Y; Fievet, P; Szymczyk, A

    2009-03-01

    The current work focuses on the application of the steric, electric, and dielectric exclusion (SEDE) model to the study of both the ion rejection rate and the membrane potential of a nanofiltration polyamide membrane. The aim of this study was to evaluate the performance of the SEDE model and to compare it with steric/electric exclusion theory. Experiments were conducted with CaCl(2) solutions at various concentrations. The SEDE model is a four-parameter model because the effective pore size (r(p)), the effective thickness-to-porosity ratio (Delta x/A(k)), the effective volume charge density of the membrane (X), and the dielectric constant of the solution inside the pores (epsilon(p)) have to be known to predict the rejection rate and the membrane potential. The first parameter was estimated from membrane potential measurements performed at high salt concentrations and the second from water permeability. In the case of single salt solutions, experimental rejection rates and membrane potentials can be described by a number of couples of values (X,epsilon(p)) because both electric and dielectric exclusion contribute to reject ions. A set of couples (X,epsilon(p)) were first estimated by fitting membrane potentials. One of the couples was found to provide a good description of experimental rejection rates as well. Results showed that the polyamide membrane is negatively charged in CaCl(2) solutions at natural pH and that the membrane charge increases with the salt concentration. A decrease in the effective dielectric constant inside the pores with respect to its bulk value was found. This is indirect evidence that dielectric exclusion plays a major role in the transport phenomena of the studied NF membrane. The standard steric/electric theory was also used to fit experimental rejection rates and membrane potentials, the effective volume charge density being the single adjustable parameter in this case. Unlike the SEDE model, the steric/electric exclusion theory was

  1. Determination of Surface Potential and Electrical Double-Layer Structure at the Aqueous Electrolyte-Nanoparticle Interface

    Directory of Open Access Journals (Sweden)

    Matthew A. Brown

    2016-01-01

    Full Text Available The structure of the electrical double layer has been debated for well over a century, since it mediates colloidal interactions, regulates surface structure, controls reactivity, sets capacitance, and represents the central element of electrochemical supercapacitors. The surface potential of such surfaces generally exceeds the electrokinetic potential, often substantially. Traditionally, a Stern layer of nonspecifically adsorbed ions has been invoked to rationalize the difference between these two potentials; however, the inability to directly measure the surface potential of dispersed systems has rendered quantitative measurements of the Stern layer potential, and other quantities associated with the outer Helmholtz plane, impossible. Here, we use x-ray photoelectron spectroscopy from a liquid microjet to measure the absolute surface potentials of silica nanoparticles dispersed in aqueous electrolytes. We quantitatively determine the impact of specific cations (Li^{+}, Na^{+}, K^{+}, and Cs^{+} in chloride electrolytes on the surface potential, the location of the shear plane, and the capacitance of the Stern layer. We find that the magnitude of the surface potential increases linearly with the hydrated-cation radius. Interpreting our data using the simplest assumptions and most straightforward understanding of Gouy-Chapman-Stern theory reveals a Stern layer whose thickness corresponds to a single layer of water molecules hydrating the silica surface, plus the radius of the hydrated cation. These results subject electrical double-layer theories to direct and falsifiable tests to reveal a physically intuitive and quantitatively verified picture of the Stern layer that is consistent across multiple electrolytes and solution conditions.

  2. Potential of Concentrating Solar Power Plants for the Combined Production of Water and Electricity in MENA Countries

    Directory of Open Access Journals (Sweden)

    Massimo Moser

    2013-06-01

    Full Text Available The widening gap between consumption and availability of water poses a serious threat to a sustainable socioeconomic development of the Middle East and North Africa (MENA countries and calls for an even larger augmentation of water supply using seawater desalination. However, these plants are affected by high specific energy consumption, while the uncertainty about fossil fuel prices in the future represents a severe problem. Within this study long-term scenarios for water and electricity demand based on potential assessment of renewable energies have been developed. The results provide baseline information for decision makers for the establishment of a favourable framework for the deployment of concentrated solar power and desalination plants. Finally, this paper points out the importance to start a paradigm change in water and electricity supply as soon as possible, in order to meet the requirements for low cost water and electricity and to avoid conflicts related to water scarcity.

  3. Electric Field and Potential Distribution in a 420 kV Novel Unibody Composite Cross-Arm

    DEFF Research Database (Denmark)

    Jahangiri, Tohid; Bak, Claus Leth; Silva, Filipe Miguel Faria da

    2015-01-01

    of shield wires. Due to a major difference between the novel cross-arm structure and traditional composite cross-arms, the electric field distribution in the uni-body composite cross-arm is of considerable interest. This paper presents and analyses the electric field distribution around and inside...... the hollow core uni-body cross-arm through which ground cable passes to connect the shield wires. Two different shed profiles are considered on the cross-arm and evaluated based on the guidelines of IEC 60815-3. The 2D geometry of pylon is modeled in ANSYS Finite Element Analysis package. The electric field...... and potential distribution along the pylon is graphically depicted and the effectiveness of assigned shed profiles in controlling the power frequency stresses are investigated in the areas with high field intensities....

  4. Atoms confined in a penetrable potential: effect of the atom position on the electric and magnetic responses

    Energy Technology Data Exchange (ETDEWEB)

    Acosta Coden, Diego S; Gomez, Sergio S; Romero, Rodolfo H, E-mail: rhromero@exa.unne.edu.ar [Instituto de Modelado e Innovacion Tecnologica, CONICET and Facultad de Ciencias Exactas y Naturales y Agrimensura, Universidad Nacional del Nordeste, Avenida Libertad 5500 (3400) Corrientes (Argentina)

    2011-02-14

    We report results of the calculation of polarizability and the nuclear magnetic shielding tensors of two-electron atoms confined within an attractive Gaussian potential well. The electric and magnetic responses are obtained within the random phase approximation (RPA) of the polarization propagator. The influence of the depth and range of the potential on the electronic structure is also studied. The dependence of the parallel (along the displacement) and perpendicular components of the polarizability and shielding tensors on the distance of the atom to the centre of the well is calculated and rationalized as a dissociation-type process of the artificial diatomic molecule formed between the Coulomb and the well potentials.

  5. A compact electric potential sensor array for the acquisition and reconstruction of the 7-lead electrocardiogram without electrical charge contact with the skin.

    Science.gov (United States)

    Harland, C J; Clark, T D; Peters, N S; Everitt, M J; Stiffell, P B

    2005-12-01

    Conventional electrocardiogram (ECG) systems make use of separate electrical connections to the arms and legs. These use a 'long baseline' for the voltage reference potential which in the case of precordial ECG leads is provided using a Wilson central terminal (WCT) wiring configuration. The aims of this project were (a) to construct compact, non-invasive surface ECG sensor arrays which would operate without the need for a WCT reference, (b) to obtain high quality precordial ECGs showing fine differences in ECG detail between small adjacent areas of the chest and (c) to reconstruct, from a compact array of four sensors, ECGs which closely match to the conventional 7-lead ECG system, but without the need for multiple wires and long baselines. In this paper, we describe two sensor array configurations which have been constructed using electric potential sensors (EPSs). We show high quality precordial ECGs obtained from small areas of the surface of the chest and show the different angular vectors (leads) in the frontal cardiac plane constructed using signals from the array elements. We suggest that these ECG arrays, which are simple to apply, should prove to be a valuable tool in providing useful information about the state of the heart.

  6. Travel behaviour of potential Electric Vehicle drivers. The need for changing

    DEFF Research Database (Denmark)

    Christensen, Linda; Nørrelund, Anders Vedsted; Olsen, Allan

    2010-01-01

    , the analyses show that it is not necessary to charge very much in periods when other costumers use the electricity but it depends on how charging is regulated. If the charging is not regulated 56 % of the normal charging of cars with 80 km driving range will take place in the day time concentrated in two peaks...... when other kind of electricity demand is also at maximum. If the charging is fully regulated only 19 % need to take place during the day as normal charging and further 19 % as fast charging. For cars with a driving range of 160 km only 4 % need to take place in daytime as normal charging and 10......The decarbonisation of road transport is one of the top priorities of policy makers in Europe. Battery powered electric vehicles are seen as one of the most promising long term sustainable options. They offer advantages in terms of energy efficiency as well as in a number of environmental aspects...

  7. Formation of photoluminescent n-type macroporous silicon: Effect of magnetic field and lateral electric potential

    Energy Technology Data Exchange (ETDEWEB)

    Antunez, E.E. [Centro de Investigación en Ingeniería y Ciencias Aplicadas, UAEM, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, CP 62210 (Mexico); Estevez, J.O. [Instituto de Física, B. Universidad Autónoma de Puebla, A.P. J-48, Puebla 72570 (Mexico); Campos, J. [Instituto de Energías Renovables, UNAM, Priv. Xochicalco S/N, Temixco, Morelos, CP 62580 (Mexico); Basurto-Pensado, M.A. [Centro de Investigación en Ingeniería y Ciencias Aplicadas, UAEM, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, CP 62210 (Mexico); Agarwal, V., E-mail: vagarwal@uaem.mx [Centro de Investigación en Ingeniería y Ciencias Aplicadas, UAEM, Av. Universidad 1001, Col. Chamilpa, Cuernavaca, Morelos, CP 62210 (Mexico)

    2014-11-15

    Metal electrode-free electrochemical etching of low doped n-type silicon substrates, under the combined effect of magnetic and lateral electric field, is used to fabricate photoluminescent n-type porous silicon structures in dark conditions. A lateral gradient in terms of structural characteristics (i.e. thickness and pore dimensions) along the electric field direction is formed. Enhancement of electric and magnetic field resulted in the increase of pore density and a change in the shape of the macropore structure, from circular to square morphology. Broad photoluminescence (PL) emission from 500 to 800 nm, with a PL peak wavelength ranging from 571 to 642 nm, is attributed to the wide range of microporous features present on the porous silicon layer.

  8. Cell surface engineering of microorganisms towards adsorption of heavy metals.

    Science.gov (United States)

    Li, Peng-Song; Tao, Hu-Chun

    2015-06-01

    Heavy metal contamination has become a worldwide environmental concern due to its toxicity, non-degradability and food-chain bioaccumulation. Conventional physical and chemical treatment methods for heavy metal removal have disadvantages such as cost-intensiveness, incomplete removal, secondary pollution and the lack of metal specificity. Microbial biomass-based biosorption is one of the approaches gaining increasing attention because it is effective, cheap, and environmental friendly and can work well at low concentrations. To enhance the adsorption properties of microbial cells to heavy metal ions, the cell surface display of various metal-binding proteins/peptides have been performed using a cell surface engineering approach. The surface engineering of Gram-negative bacteria, Gram-positive bacteria and yeast towards the adsorption of heavy metals are reviewed in this article. The problems and future perspectives of this technology are discussed.

  9. Cell-surface acceleration of urokinase-catalyzed receptor cleavage

    DEFF Research Database (Denmark)

    Høyer-Hansen, G; Ploug, M; Behrendt, N

    1997-01-01

    The urokinase-type plasminogen activator (uPA) binds to a specific cell-surface receptor, uPAR. On several cell types uPAR is present both in the full-length form and a cleaved form, uPAR(2+3), which is devoid of binding activity. The formation of uPAR(2+3) on cultured U937 cells is either direct...

  10. Cell Surface-associated Proteins of Gastrointestinal Strains of Lactobacilli

    OpenAIRE

    Chagnaud, P.; Jenkinson, H. F.; Tannock, G. W.

    2011-01-01

    Twenty-eight strains of gastrointestinal lactobacilli, comprising isolates classified as Lactobacillus reuteri, L. acidophilus, L. delbrueckii, L. fermentum, L. gasseri and L. salivarius, were examined for the presence of cell-surface polypeptides. Whole cells were radioactively labelled with 125I using lactoperoxidase or chloramine T as catalyst, proteins were extracted with sodium dodecyl sulphate (SDS) and separated by SDS-polyacrylamide gel electrophoresis. All lactobacilli tested had bet...

  11. Engineering novel cell surface chemistry for selective tumor cell targeting

    Energy Technology Data Exchange (ETDEWEB)

    Bertozzi, C.R. [Univ. of California, Berkeley, CA (United States)]|[Lawrence Berkeley National Lab., CA (United States)

    1997-12-31

    A common feature of many different cancers is the high expression level of the two monosaccharides sialic acid and fucose within the context of cell-surface associated glycoconjugates. A correlation has been made between hypersialylation and/or hyperfucosylation and the highly metastatic phenotype. Thus, a targeting strategy based on sialic acid or fucose expression would be a powerful tool for the development of new cancer cell-selective therapies and diagnostic agents. We have discovered that ketone groups can be incorporated metabolically into cell-surface associated sialic acids. The ketone is can be covalently ligated with hydrazide functionalized proteins or small molecules under physiological conditions. Thus, we have discovered a mechanism to selectively target hydrazide conjugates to highly sialylated cells such as cancer cells. Applications of this technology to the generation of novel cancer cell-selective toxins and MRI contrast reagents will be discussed, in addition to progress towards the use of cell surface fucose residues as vehicles for ketone expression.

  12. Mapping cell surface adhesion by rotation tracking and adhesion footprinting

    Science.gov (United States)

    Li, Isaac T. S.; Ha, Taekjip; Chemla, Yann R.

    2017-03-01

    Rolling adhesion, in which cells passively roll along surfaces under shear flow, is a critical process involved in inflammatory responses and cancer metastasis. Surface adhesion properties regulated by adhesion receptors and membrane tethers are critical in understanding cell rolling behavior. Locally, adhesion molecules are distributed at the tips of membrane tethers. However, how functional adhesion properties are globally distributed on the individual cell’s surface is unknown. Here, we developed a label-free technique to determine the spatial distribution of adhesive properties on rolling cell surfaces. Using dark-field imaging and particle tracking, we extract the rotational motion of individual rolling cells. The rotational information allows us to construct an adhesion map along the contact circumference of a single cell. To complement this approach, we also developed a fluorescent adhesion footprint assay to record the molecular adhesion events from cell rolling. Applying the combination of the two methods on human promyelocytic leukemia cells, our results surprisingly reveal that adhesion is non-uniformly distributed in patches on the cell surfaces. Our label-free adhesion mapping methods are applicable to the variety of cell types that undergo rolling adhesion and provide a quantitative picture of cell surface adhesion at the functional and molecular level.

  13. Influence of the external conditions on salt retention and pressure-induced electrical potential measured across a composite membrane

    DEFF Research Database (Denmark)

    Benavente, Juana; Jonsson, Gunnar Eigil

    1999-01-01

    Transport on single electrolyte solutions (NaCl and MgCl2) due to pressure gradients across a commercial reverse osmosis membrane was studied by measuring volume flux (J(v)), salt rejection (S) and pressure induced electrical potential (Delta E) in a crossflow cell. The influence on these paramet......Transport on single electrolyte solutions (NaCl and MgCl2) due to pressure gradients across a commercial reverse osmosis membrane was studied by measuring volume flux (J(v)), salt rejection (S) and pressure induced electrical potential (Delta E) in a crossflow cell. The influence...... with respect to the value determined with single electrolytes at the same concentration was obtained, which is attributed to a strong coupling among the fluxes of individual ions and their distribution in the membrane when transport of mixed salt is studied. (C) 1999 Elsevier Science B.V. All rights reserved....

  14. CO2Mitigation Potential of Plug-in Hybrid Electric Vehicles larger than expected.

    Science.gov (United States)

    Plötz, P; Funke, S A; Jochem, P; Wietschel, M

    2017-11-28

    The actual contribution of plug-in hybrid and battery electric vehicles (PHEV and BEV) to greenhouse gas mitigation depends on their real-world usage. Often BEV are seen as superior as they drive only electrically and do not have any direct emissions during driving. However, empirical evidence on which vehicle electrifies more mileage with a given battery capacity is lacking. Here, we present the first systematic overview of empirical findings on actual PHEV and BEV usage for the US and Germany. Contrary to common belief, PHEV with about 60 km of real-world range currently electrify as many annual vehicles kilometres as BEV with a much smaller battery. Accordingly, PHEV recharged from renewable electricity can highly contribute to green house gas mitigation in car transport. Including the higher CO 2eq emissions during the production phase of BEV compared to PHEV, PHEV show today higher CO 2eq savings then BEVs compared to conventional vehicles. However, for significant CO 2eq improvements of PHEV and particularly of BEVs the decarbonisation of the electricity system should go on.

  15. Estimating potential stranded commitments for U.S. investor-owned electric utilities

    Energy Technology Data Exchange (ETDEWEB)

    Baxter, L.; Hirst, E.

    1995-01-01

    New technologies, low natural gas prices, and federal and state utility regions are restructuring the electricity industry. Yesterday`s vertically integrated utility with a retail monopoly franchise may be a very different organization in a few years. Conferences, regulatory-commission hearings, and other industry fora are dominated by debates over the extent and form of utility deintegration, wholesale competition, and retail wheeling. A key obstacle to restructuring the electricity industry is stranded commitments. Past investments, power-purchase contracts, and public-policy-driven programs that made sense in an era of cost-of-service regulation may not be cost-effective in a competitive power market. Regulators, utilities, and other parties face tough decisions concerning the mitigation and allocation of these stranded commitments. The authors developed and applied a simple method to calculate the amount of stranded commitments facing US investor-owned electric utilities. The results obtained with this method depend strongly on a few key assumptions: (1) the fraction of utility sales that is at risk with respect to competition, (2) the market price of electric generation, and (3) the number of years during which the utility would lose money because of differences between its embedded cost of production and the market price.

  16. Swiss pumped hydro storage potential for Germany's electricity system under high penetration of intermittent renewable energy

    NARCIS (Netherlands)

    van Meerwijk, Aagje J. H.; Benders, Reinerus; Davila-Martinez, Alejandro; Laugs, Gideon A. H.

    2016-01-01

    In order to cut greenhouse-gas emissions and increase energy security, the European Commission stimulates the deployment of intermittent renewable energy sources (IRES) towards 2050. In an electricity system with high shares of IRES implemented in the network, energy balancing like storage is needed

  17. Effect of pulsed electric field treatment on hot-boned muscles of different potential tenderness.

    Science.gov (United States)

    Suwandy, Via; Carne, Alan; van de Ven, Remy; Bekhit, Alaa El-Din A; Hopkins, David L

    2015-07-01

    In this study, the effect of pulsed electric field (PEF) treatment and ageing on the quality of beef M. longissimus lumborum (LL) and M. semimembranosus (SM) muscles was evaluated, including the tenderness, water loss and post-mortem proteolysis. Muscles were obtained from 12 steers (6 steers for each muscle), removed from the carcasses 4 hour postmortem and were treated with pulsed electric field within 2h. Six different pulsed electric field intensities (voltages of 5 and 10 kV × frequencies of 20, 50 and 90 Hz) plus a control were applied to each muscle to determine the optimum treatment conditions. Beef LL was found to get tougher with increasing treatment frequency whereas beef SM muscle was found to have up to 21.6% reduction in the shear force with pulsed electric field treatment. Post-mortem proteolysis showed an increase in both troponin and desmin degradation in beef LL treated with low intensity PEF treatment (20 Hz) compared to non-treated control samples. Copyright © 2015 Elsevier Ltd. All rights reserved.

  18. A meta-analytic study of exogenous oscillatory electric potentials in neuroenhancement

    NARCIS (Netherlands)

    Schutter, D.J.L.G.; Wischnewski, M.

    2016-01-01

    The assumption that transcranial alternating current stimulation (tACS) enhances perceptual and cognitive ability in healthy volunteers by exposing the brain to exogenous oscillatory electric fields is increasingly finding its way into society and commercial parties. The aim of the present study is

  19. Smart grids in the colombian electric system: Current situation and potential opportunities

    Directory of Open Access Journals (Sweden)

    William Mauricio Giral Ramírez

    2017-07-01

    Full Text Available Context: This paper focuses on providing a functional analysis of smart grids, with the purpose of establishing a framework to identify the main characteristics of the current electric interconnection system in Colombia. It also names the positive incentives proposed by the Colombian government to support both research and development projects that implement non-conventional energy sources and promoting energy management based on efficiency. Method: An architecture model that describes the components interoperability of a smart grid is presented using a descriptive methodology. Results: The results include a list of the objectives established by the Colombian public and private entities related to energy development, specially focusing on the opportunities to provide some kind of artificial intelligence to the current electrical system. Conclusions: It is necessary for the Colombian energy system to supply the energy demand considering electrical safety, social equity, and the minimum environmental impact. These restrictions impose new challenges for the energy system itself: From a technical point of view, the traditional electrical grid must be outfitted with the characteristics of a smart grid, and from a legal perspective, it is essential to generate a clear regulatory framework that promotes the development of this type of technology.

  20. Nitrogen-deficient microalgae are rich in cell-surface mannose: potential implications for prey biorecognition by phagotrophic protozoa Microalgas com deficiência de nitrogênio são enriquecidas com manose na superfície celular: potenciais implicações para reconhecimento de presas por protozoários fagotróficos

    Directory of Open Access Journals (Sweden)

    Claire M. Martel

    2009-03-01

    Full Text Available Flow cytometry was used to quantify the abundance of mannose-linked glycoconjugates on microalgae precultured using low- or high-nitrate media. Nitrogen-deficient microalgae were richer in cell-surface mannose than nitrogen-sufficient. Findings are discussed in view of recent research which reveals mannose-specific 'feeding receptors' assist prey biorecognition by phagotrophic protozoa that ingest microalgae.Citometria de fluxo foi usada para quantificar a abundância de glicoconjugados com manose em precultivos de microalgas usando meios com baixo e alto teor de nitrato. Microalgas com deficiências de nitrogênio tinham mais manose na superfície celular do que as com nitrogênio suficiente. Resultados são discutidos com base nas pesquisas recentes que revelam receptores específicos para manose que auxiliam no reconhecimento da presa por protozoários fagotróficos que ingerem microalgas.

  1. Unmasking local activity within local field potentials (LFPs) by removing distal electrical signals using independent component analysis.

    Science.gov (United States)

    Whitmore, Nathan W; Lin, Shih-Chieh

    2016-05-15

    Local field potentials (LFPs) are commonly thought to reflect the aggregate dynamics in local neural circuits around recording electrodes. However, we show that when LFPs are recorded in awake behaving animals against a distal reference on the skull as commonly practiced, LFPs are significantly contaminated by non-local and non-neural sources arising from the reference electrode and from movement-related noise. In a data set with simultaneously recorded LFPs and electroencephalograms (EEGs) across multiple brain regions while rats perform an auditory oddball task, we used independent component analysis (ICA) to identify signals arising from electrical reference and from volume-conducted noise based on their distributed spatial pattern across multiple electrodes and distinct power spectral features. These sources of distal electrical signals collectively accounted for 23-77% of total variance in unprocessed LFPs, as well as most of the gamma oscillation responses to the target stimulus in EEGs. Gamma oscillation power was concentrated in volume-conducted noise and was tightly coupled with the onset of licking behavior, suggesting a likely origin of muscle activity associated with body movement or orofacial movement. The removal of distal signal contamination also selectively reduced correlations of LFP/EEG signals between distant brain regions but not within the same region. Finally, the removal of contamination from distal electrical signals preserved an event-related potential (ERP) response to auditory stimuli in the frontal cortex and also increased the coupling between the frontal ERP amplitude and neuronal activity in the basal forebrain, supporting the conclusion that removing distal electrical signals unmasked local activity within LFPs. Together, these results highlight the significant contamination of LFPs by distal electrical signals and caution against the straightforward interpretation of unprocessed LFPs. Our results provide a principled approach to

  2. Insulin resistance selectively alters cell-surface glucose transporters but not their total protein expression in equine skeletal muscle.

    Science.gov (United States)

    Waller, A P; Burns, T A; Mudge, M C; Belknap, J K; Lacombe, V A

    2011-01-01

    Insulin resistance (IR) has been widely recognized in humans, and more recently in horses, but its underlying mechanisms are still not well understood. The translocation of glucose transporter 4 (GLUT4) to the cell surface is the limiting step for glucose uptake in insulin-sensitive tissues. Although the downstream signaling pathways regulating GLUT translocation are not well defined, AS160 recently has emerged as a potential key component. In addition, the role of GLUT12, one of the most recently identified insulin-sensitive GLUTs, during IR is unknown. We hypothesized that cell-surface GLUT will be decreased in muscle by an AS160-dependent pathway in horses with IR. Insulin-sensitive (IS) or IR mares (n = 5/group). Muscle biopsies were performed in mares classified as IS or IR based on results of an insulin-modified frequently sampled IV glucose tolerance test. By an exofacial bis-mannose photolabeled method, we specifically quantified active cell-surface GLUT4 and GLUT12 transporters. Total GLUT4 and GLUT12 and AS160 protein expression were measured by Western blots. IR decreased basal cell-surface GLUT4 expression (P= .027), but not GLUT12, by an AS160-independent pathway, without affecting total GLUT4 and GLUT12 content. Cell-surface GLUT4 was not further enhanced by insulin stimulation in either group. IR induced defects in the skeletal muscle glucose transport pathway by decreasing active cell-surface GLUT4. Copyright © 2011 by the American College of Veterinary Internal Medicine.

  3. Adsorption of glycosaminoglycans to the cell surface is responsible for cellular donnan effects.

    Science.gov (United States)

    Hagenfeld, Daniel; Kathagen, Nadine; Prehm, Peter

    2014-07-01

    In previous publications, we showed that extracellular glycosaminoglycans reduced the membrane potential, caused cell blebbing and swelling and decreased the intracellular pH independently of cell surface receptors. These phenomena were explained by Donnan effects. The effects were so large that they could not be attributed to glycosaminoglycans in solution. Therefore, we tested the hypothesis that glycosaminoglycans were concentrated on the cell membrane and analysed the mechanism of adsorption by fluorescent hyaluronan, chondroitin sulphate and heparin. The influence of the CD44 receptor was evaluated by comparing CD44 expressing human fibroblasts with CD44 deficient HEK cells. Higher amounts of glycosaminoglycans adsorbed to fibroblasts than to HEK cells. When the membrane potential was annihilated by substituting NaCl by KCl in the medium, adsorption was reduced and intracellular pH decrease was abolished. To eliminate other cellular interfering factors, potential-dependent adsorption was demonstrated for hyaluronan which adsorbed to inert gold foils in physiological salt concentrations at pH 7.2 and surface potentials up to 120 mV. From these results, we conclude that large cellular Donnan effects of glycosaminoglycans results from receptor mediated, hydrophobic and ionic adsorption to cell surfaces. © 2014 Wiley Periodicals, Inc.

  4. Observation of electric potential in organic thin-film transistor by bias-applied hard X-ray photoemission spectroscopy

    Science.gov (United States)

    Watanabe, Takeshi; Tada, Keisuke; Yasuno, Satoshi; Oji, Hiroshi; Yoshimoto, Noriyuki; Hirosawa, Ichiro

    2016-03-01

    The effect of gate voltage on electric potential in a pentacene (PEN) layer was studied by hard X-ray photoelectron spectroscopy under a bias voltage. It was observed that applying a negative gate voltage substantially increases the width of a C 1s peak. This suggested that injected and accumulated carriers in an organic thin film transistor channel modified the potential depth profile in PEN. It was also observed that the C 1s kinetic energy tends to increase monotonically with threshold voltage.

  5. Improved accuracy of cell surface shaving proteomics in Staphylococcus aureus using a false-positive control

    DEFF Research Database (Denmark)

    Solis, Nestor; Larsen, Martin Røssel; Cordwell, Stuart J

    2010-01-01

    Proteolytic treatment of intact bacterial cells is an ideal means for identifying surface-exposed peptide epitopes and has potential for the discovery of novel vaccine targets. Cell stability during such treatment, however, may become compromised and result in the release of intracellular proteins...... that complicate the final analysis. Staphylococcus aureus is a major human pathogen, causing community and hospital-acquired infections, and is a serious healthcare concern due to the increasing prevalence of multiple antibiotic resistances amongst clinical isolates. We employed a cell surface "shaving" technique...

  6. Proteomic analysis of cell surface-associated proteins from probiotic Lactobacillus plantarum

    DEFF Research Database (Denmark)

    Beck, Hans Christian; Madsen, Søren M; Glenting, Jacob

    2009-01-01

    In the present study, we used a proteomic approach to identify surface-associated proteins from the probiotic bacterium Lactobacillus plantarum 299v. Proteins were extracted from the cell surface using a mild wash in phosphate buffer and analysed by sodium dodecyl sulphate-polyacrylamide gel...... electrophoresis. Gel bands were excised and in-gel digested with trypsin. The resulting peptides were analysed by capillary-LC-ESI-MS/MS. The peptide sequences were used for a database search and allowed identification of a total of 29 proteins, many of which could potentially be involved in the action...

  7. The effect of the electrical double layer on hydrodynamic lubrication: a non-monotonic trend with increasing zeta potential

    Directory of Open Access Journals (Sweden)

    Dalei Jing

    2017-07-01

    Full Text Available In the present study, a modified Reynolds equation including the electrical double layer (EDL-induced electroviscous effect of lubricant is established to investigate the effect of the EDL on the hydrodynamic lubrication of a 1D slider bearing. The theoretical model is based on the nonlinear Poisson–Boltzmann equation without the use of the Debye–Hückel approximation. Furthermore, the variation in the bulk electrical conductivity of the lubricant under the influence of the EDL is also considered during the theoretical analysis of hydrodynamic lubrication. The results show that the EDL can increase the hydrodynamic load capacity of the lubricant in a 1D slider bearing. More importantly, the hydrodynamic load capacity of the lubricant under the influence of the EDL shows a non-monotonic trend, changing from enhancement to attenuation with a gradual increase in the absolute value of the zeta potential. This non-monotonic hydrodynamic lubrication is dependent on the non-monotonic electroviscous effect of the lubricant generated by the EDL, which is dominated by the non-monotonic electrical field strength and non-monotonic electrical body force on the lubricant. The subject of the paper is the theoretical modeling and the corresponding analysis.

  8. Electrical heating tapes, their use, energy consumption and energy savings potential; Elektrische Heizbaender. Anwendungen, Energieverbrauch und Sparmoeglichkeiten

    Energy Technology Data Exchange (ETDEWEB)

    Nipkow, J. [Arena, Zuerich (Switzerland); Lingenhel, S. [E Viva, Basel (Switzerland)

    2002-07-01

    Heating cables require about 0.6% of Swiss electricity consumption, approximately as much as all TV-sets. Most important applications are domestic hot water distribution tubing, frost protection (tubing, gutters) and industrial tubing, each requiring about 1/3. For over 10 years, self-regulating technology is standard for heating cables and offers pre-defined maintenance temperatures. To minimize electricity consumption, in most applications additional control devices (timer, temperature-/ power control) are necessary. The study could not give a general answer to whether domestic hot water distribution systems should be heated by heating cables or circulation systems. The best solution depends on the specific building circumstances. Conclusions of the technical and market analysis say that measures in different fields can transfer the saving potentials into practice: architects and designers of sanitary and electrical installations should be informed by articles in specialized magazines: the goal is either to avoid the use of heating cables or to minimize their electricity consumption, training of plumbers and electricians should treat the efficient use of heating cables. An instruction leaflet is to be created. Building owners and operators should be informed by their specialized magazines about problems with heating cables. (author)

  9. A statistical analysis of electric self-potential time series associated to two 1993 earthquakes in Mexico

    Directory of Open Access Journals (Sweden)

    L. Flores-Márquez

    2007-09-01

    Full Text Available Recent studies related with earthquake prediction involve statistical studies of the ground electric self-potential behavior. Published results about the complexity of this kind of processes encourage us to study the statistical behavior of the ground electric self-potential recorded in Guerrero state, Mexico. This region is characterized by high seismicity. The electric self-potential variations were recorded in the Acapulco station directly from the ground. The sampling period was four seconds and the data were stored from March to December of 1993. Two significant earthquakes (EQs occurred near this station, 15 May and 24 October whose magnitudes were Mw=6.0 and Mw=6.6 respectively. A preliminary processing was carried out consisting of a moving average of the original time series in order to filter the very high frequencies and to complete short lacks of data and outliers. Then, a visual inspection of the complete filtered signal was performed to search some seismic electric signals (SES, which were ambiguously depicted. Subsequently, a detrending of µ=0 was applied with the windows of 3.3, 6.6 and 10 h. Later, the analysis of the spectral exponent β was made, showing changes during the total period examined, and the most evident changes occurred during the preparation mechanism of the Mw=6.6 EQ. Fifteen days before the 24 October EQ, a Brownian-noise like behavior was displayed (β≈2, having a duration of about two days. In addition a Higuchi fractal method and wavelet analysis were made confirming the presence of the β-anomaly.

  10. Potential Benefits from Improved Energy Efficiency of KeyElectrical Products: The Case of India

    Energy Technology Data Exchange (ETDEWEB)

    McNeil, Michael; Iyer, Maithili; Meyers, Stephen; Letschert,Virginie; McMahon, James E.

    2005-12-20

    The goal of this project was to estimate the net benefits that cost-effective improvements in energy efficiency can bring to developing countries. The study focused on four major electrical products in the world's second largest developing country, India. These products--refrigerators, room air conditioners, electric motors, and distribution transformers--are important targets for efficiency improvement in India and in other developing countries. India is an interesting subject of study because of it's size and rapid economic growth. Implementation of efficient technologies in India would save billions in energy costs, and avoid hundreds of megatons of greenhouse gas emissions. India also serves as an example of the kinds of improvement opportunities that could be pursued in other developing countries.

  11. Modeling of Potential Distribution of Electrical Capacitance Tomography Sensor for Multiphase Flow Image

    Directory of Open Access Journals (Sweden)

    S. Sathiyamoorthy

    2007-09-01

    Full Text Available Electrical Capacitance Tomography (ECT was used to develop image of various multi phase flow of gas-liquid-solid in a closed pipe. The principal difficulties to obtained real time image from ECT sensor are permittivity distribution across the plate and capacitance is nonlinear; the electric field is distorted by the material present and is also sensitive to measurement errors and noise. This work present a detailed description is given on method employed for image reconstruction from the capacitance measurements. The discretization and iterative algorithm is developed for improving the predictions with minimum error. The author analyzed eight electrodes square sensor ECT system with two-phase water-gas and solid-gas.

  12. Evaluation of Constant Potential Method in Simulating Electric Double-Layer Capacitors

    OpenAIRE

    Wang, Zhenxing; Yang, Yang; Olmsted, David L.; Asta, Mark; Laird, Brian B.

    2014-01-01

    A major challenge in the molecular simulation of electric double layer capacitors (EDLCs) is the choice of an appropriate model for the electrode. Typically, in such simulations the electrode surface is modeled using a uniform fixed charge on each of the electrode atoms, which ignores the electrode response to local charge fluctuations induced by charge fluctuations in the electrolyte. In this work, we evaluate and compare this Fixed Charge Method (FCM) with the more realistic Constant Potent...

  13. Effects of connection of electrical and mechanical potentials in inverse osmosis processes

    Energy Technology Data Exchange (ETDEWEB)

    Cortes, Farid; Chejne, Farid; Chejne, David; Velez, Fredy; Londono, Carlos [Grupo de Termodinamica Aplicada y Energias Alternativas - TAYEA, Instituto de Energia, Facultad de Minas, Universidad Nacional de Colombia, Sede Medellin, Antigua (Colombia)

    2009-07-15

    A theoretical dissertation and experimental assays of the irreversible phenomena applied to electro-kinetics and inverse osmosis is presented. Experimental assays were made on simple equipment to evidence the occurrence of connected irreversible phenomena between electric current flow and global mass flow. The coupling of these two phenomena allowed us to make conclusions about the possibility of reducing operation costs of the inverse osmosis equipment due to increasing the saline solution flow between 12% and 20%. (author)

  14. Assessing the Potential of Plug-in Electric Vehicles in Active Distribution Networks

    DEFF Research Database (Denmark)

    Kordheili, Reza Ahmadi; Pourmousavi, Seyyed Ali; Savaghebi, Mehdi

    2016-01-01

    A multi-objective optimization algorithm is proposed in this paper to increase the penetration level of renewable energy sources (RESs) in distribution networks by intelligent management of plug-in electric vehicle (PEV) storage. The proposed algorithm is defined to manage the reverse power flow ...... demonstrate the capability of the algorithm in increasing solar power penetration in the grid up to 50%, depending on the PEV penetration level and the freedom of the system operator in managing the available PEV storage....

  15. Electric industry restructuring, ancillary services, and the potential impact on wind

    Energy Technology Data Exchange (ETDEWEB)

    Kirby, B.; Hirst, E. [Oak Ridge National Lab., TN (United States); Parsons, B.; Porter, K. [National Renewable Energy Lab., Golden, CO (United States)] [and others

    1997-12-31

    The new competitive electric power environment raises increased challenges for wind power. The DOE and EPRI wind programs have dealt extensively with the traditional vertically integrated utility planning and operating environment in which the host utility owns the generation (or purchases the power) and provides dispatch and transmission services. Under this traditional environment, 1794 MW of wind power, principally in California, have been successfully integrated into the U.S. electric power system. Another 4200 MW are installed elsewhere in the world. As issues have arisen, such as intermittency and voltage regulation, they have been successfully addressed with accepted power system procedures and practices. For an intermittent, non-dispatchable resource such as wind, new regulatory rules affecting power transmission services, raise questions about which ancillary services wind plants will be able to sell, which they will be required to purchase, and what the economic impacts will be on individual wind projects. This paper begins to look at issues of concern to wind in a restructured electric industry. The paper first briefly looks at the range of unbundled services and comments on their unique significance to wind. To illustrate the concerns that arise with restructuring, the paper then takes a more detailed look at a single service: regulation. Finally, the paper takes a brief look at technologies and strategies that could improve the competitive position of wind.

  16. The potential for western Canada to become a leader in electrically powered land transport

    Energy Technology Data Exchange (ETDEWEB)

    Perl, A.; Gilbert, R. [Simon Fraser Univ., Burnaby, BC (Canada)

    2010-07-01

    Canada's western provinces are among the most oil-intensive jurisdictions in the world. Per capita consumption in 2007 was 70 percent higher than that of eastern Canada. While western Canada will be buffered as a producer of oil, predicted swings in oil prices may disrupt the fiscal capacity of producer provinces. The price of crude oil in western Canada is the same price established in world markets. This paper examined some of the opportunities available for western Canada to move away from oil consumption by becoming a leader in electric traction. Methods of replacing oil-based transportation with the use of electric vehicles were discussed. Western Canada's capacity to produce electricity from renewable resources was evaluated. Economic opportunities related to the electrification of land transport were also examined. The report concluded by stating that a clear vision and policy framework for supporting new technologies for surface transport across western Canada are needed. 72 refs., 4 figs.

  17. Potential growth of nuclear and coal electricity generation in the US

    Energy Technology Data Exchange (ETDEWEB)

    Bloomster, C.H.; Merrill, E.T.

    1989-08-01

    Electricity demand should continue to grow at about the same rate as GNP, creating a need for large amounts of new generating capacity over the next fifty years. Only coal and nuclear at this time have the abundant domestic resources and assured technology to meet this need. However, large increase in both coal and nuclear usage will require solutions to many of the problems that now deter their increased usage. For coal, the problems center around the safety and environmental impacts of increased coal mining and coal combustion. For nuclear, the problems center around reactor safety, radioactive waste disposal, financial risk, and nuclear materials safeguards. This report assesses the impacts associated with a range of projected growth rates in electricity demand over the next 50 years. The resource requirements and waste generation resulting from pursuing the coal and nuclear fuel options to meet the projected growth rates are estimated. The fuel requirements and waste generation for coal plants are orders of magnitude greater than for nuclear. Improvements in technology and waste management practices must be pursued to mitigate environmental and safety concerns about electricity generation from both options. 34 refs., 18 figs., 14 tabs.

  18. On the detectability of transverse cracks in laminated composites through measurements of electrical potential change

    KAUST Repository

    Selvakumaran, Lakshmi

    2015-01-07

    For structures made of laminated composites, real-time structural health monitoring is necessary as significant damage may occur without any visible signs on the surface. Inspection by electrical tomography seems a viable approach as the technique relies on voltage measurements from a network of electrodes over the boundary of the inspected domain to infer the change in conductivity within the bulk material. The change in conductivity, if significant, can be correlated to the degradation state of the material, allowing damage detection. We focus here on the detection of the transverse cracking mechanism which modifies the in-plane transverse conductivity of ply. The quality of detection is directly related to the sensitivity of the voltage measurements with respect to the presence of cracks. We demonstrate here from numerical experiments that the sensitivity depends on several parameters, such as the anisotropy in the electrical conductivity of the baseline composite ply or the geometricalparameters of the structure. Based on these results, applicability of electrical tomography to detect transverse cracks in a laminate is discussed.

  19. Exploring new scaling regimes for streaming potential and electroviscous effects in a nanocapillary with overlapping electric double layers.

    Science.gov (United States)

    Das, Siddhartha; Guha, Arnab; Mitra, Sushanta K

    2013-12-04

    In this paper, we unravel new scaling regimes for streaming potential and electroviscous effects in a nanocapillary with thick overlapping Electric Double Layers (EDLs). We observe that the streaming potential, for a given value of the capillary zeta (ζ) potential, varies with the EDL thickness and a dimensionless parameter R, quantifying the conduction current. Depending on the value of R, variation of the streaming potential with the EDL thickness demonstrates distinct scaling regimes: one can witness a Quadratic Regime where the streaming potential varies as the square of the EDL thickness, a Weak Regime where the streaming potential shows a weaker variation with the EDL thickness, and a Saturation Regime where the streaming potential ceases to vary with the EDL thickness. Effective viscosity, characterizing the electroviscous effect, obeys the variation of the streaming potential for smaller EDL thickness values; however, for larger EDL thickness the electroosmotic flow profile dictates the electroviscous effect, with insignificant contribution of the streaming potential. Copyright © 2013 Elsevier B.V. All rights reserved.

  20. Storing unsteady energy, like photovoltaically generated electric energy, as potential energy

    OpenAIRE

    Kutz, Nadja

    2012-01-01

    A proposal to store unsteady energy in potential energy via lifting masses with a rough quantitative overview. Some applications and methods to harvest the potential energy are also given. A focus is put on photovoltaically generated energy.

  1. Spatial distribution of the electrical potential and ion concentration in the downstream area of atmospheric pressure remote plasma

    Directory of Open Access Journals (Sweden)

    M. V. Mishin

    2014-10-01

    Full Text Available This paper presents the results from an experimental study of the ion flux characteristics behind the remote plasma zone in a vertical tube reaction chamber for atmospheric pressure plasma enhanced chemical vapor deposition. Capacitively coupled radio frequency plasma was generated in pure He and gas mixtures: He–Ar, He–O2, He–TEOS. We previously used the reaction system He–TEOS for the synthesis of self-assembled structures of silicon dioxide nanoparticles. It is likely that the electrical parameters of the area, where nanoparticles have been transported from the synthesis zone to the substrate, play a significant role in the self-organization processes both in the vapor phase and on the substrate surface. The results from the spatial distribution of the electrical potential and ion concentration in the discharge downstream area measured by means of the external probe of original design and the special data processing method are demonstrated in this work. Positive and negatives ions with maximum concentrations of 106–107 cm−3 have been found at 10–80 mm distance behind the plasma zone. On the basis of the revealed distributions for different gas mixtures, the physical model of the observed phenomena is proposed. The model illustrates the capability of the virtual ion emitter formation behind the discharge gap and the presence of an extremum of the electrical potential at the distance of approximately 10−2–10−1 mm from the grounded electrode.

  2. The Green Electricity: The Potential of Using Solar Pv Panel for The Royal Malaysian Police (RMP Building

    Directory of Open Access Journals (Sweden)

    Md Yasin Md Hafiz

    2016-01-01

    Full Text Available As the year 2020 approaches, Malaysia aims to be self-sufficient and independence in finding resources to fuel its development, especially in energy sector. To achieve this aims, Malaysia need to strengthen its energy sustainability aspect and have a clear foresight in handling energy security issue. In coming decades, energy crisis due the existence of the significant energy threat may cause power interruptions and lead to many interrelated problems, especially safety and security issues. Issues such as unstable political and economic situation in Middle East country, Spratly issue, South China Sea territorial issue, war and terrorist may lead to unstable safety and security environment for Malaysia. The most significant organization to keep the safety and security of Malaysia is The Royal Malaysian Police (RMP. However, at the moment, this organization is fully-reliant on centralized electricity supply that may interrupt due to the future energy threat. Therefore, it is important for this organization to have an independence and self-sufficient electricity supply in order to remain their operation in any circumstances .The solar energy, through Photovoltaic (PV panel, has been identified as the significant resources to achieve this purposes. With constant solar irradiance, Malaysia has great potential in solar energy harvesting. The energy is clean, environmental-friendly, reliable and readily-accessible throughout the year. This paper will review the potential of green electricity through solar PV in being the power supply for RMP in order to maintain the security and safety of Malaysia

  3. The Boom of Electricity Demand in the Residential Sector in the Developing World and the Potential for Energy Efficiency

    Energy Technology Data Exchange (ETDEWEB)

    Letschert, Virginie; McNeil, Michael A.

    2008-05-13

    With the emergence of China as the world's largest energy consumer, the awareness of developing country energy consumption has risen. According to common economic scenarios, the rest of the developing world will probably see an economic expansion as well. With this growth will surely come continued rapid growth in energy demand. This paper explores the dynamics of that demand growth for electricity in the residential sector and the realistic potential for coping with it through efficiency. In 2000, only 66% of developing world households had access to electricity. Appliance ownership rates remain low, but with better access to electricity and a higher income one can expect that households will see their electricity consumption rise significantly. This paper forecasts developing country appliance growth using econometric modeling. Products considered explicitly - refrigerators, air conditioners, lighting, washing machines, fans, televisions, stand-by power, water heating and space heating - represent the bulk of household electricity consumption in developing countries. The resulting diffusion model determines the trend and dynamics of demand growth at a level of detail not accessible by models of a more aggregate nature. In addition, the paper presents scenarios for reducing residential consumption through cost-effective and/or best practice efficiency measures defined at the product level. The research takes advantage of an analytical framework developed by LBNL (BUENAS) which integrates end use technology parameters into demand forecasting and stock accounting to produce detailed efficiency scenarios, which allows for a realistic assessment of efficiency opportunities at the national or regional level. The past decades have seen some of the developing world moving towards a standard of living previously reserved for industrialized countries. Rapid economic development, combined with large populations has led to first China and now India to emerging as &apos

  4. Effects of microwave electric fields on the translational diffusion of dipolar molecules in surface potential: A simulation study

    Science.gov (United States)

    Kapranov, Sergey V.; Kouzaev, Guennadi A.

    2018-01-01

    Variations of effective diffusion coefficient of polar molecules exposed to microwave electric fields in a surface potential are studied by solving coupled stochastic differential equations of motion with a deterministic component of the surface force. Being an essential tool for the simulation interpretation, a theoretical approach to effective diffusion in surface potential is first developed. The effective diffusion coefficient is represented as the product of the normal diffusion coefficient and potential-dependent correction function, whose temperature dependence is close to the Arrhenius form. The analytically found zero-diffusion condition defines the state of thermal equilibrium at the surface. The diffusion of a water-like dipole molecule in the potential of graphite surface is simulated in the field-free conditions and in the presence of the alternating electric fields of various magnitude intensities and frequencies. Temperature dependence of the correction function exhibits field-induced variations of the effective Lennard-Jones energy parameter. It demonstrates maximum departure from the zero-field value at certain frequencies and intensities, which is associated with variations in the rotational dynamics. A concept of the amplitude-frequency resonance put forward to interpret the simulation results is explained using a heuristic reasoning and is corroborated by semi-quantitative considerations in terms of the Dissado-Hill cluster theory of dielectric relaxation.

  5. The Use Of Electromagnetic And Electrical Resistivity Methods In Assessing Groundwater Resource Potentials In Adoe Sunyani Ghana.

    Directory of Open Access Journals (Sweden)

    Alfred K. Bienibuor

    2015-08-01

    Full Text Available Electromagnetic and electrical resistivity geophysical methods were used to map out potential groundwater sites for boreholes drilling in the Adoe community in the Sunyani west district of Ghana. The electromagnetic data was taken with the Geonics EM-34 conductivity meter while the electrical resistivity data was taken with the ABEM SAS 1000 C Terrameter using the Schlumberger electrode configuration. Results from the measurements revealed four subsurface geological layers of the following resistivity and thickness ranges quartzitic sandstone with clay 42-118 amp937m 1-2.2 m sandy clay with silt 27-487 amp937m 9-12 m lateritic sandstone 13-728 amp937m 6-14 m and clayey shale 20-29 amp937m 6-14 m The overburden ranged in thickness from 14 m to 24 m. Sites selected for borehole drilling had a groundwater yield range of 0.94 -12 m3h.

  6. The development of the experimental setup for measuring the cell membrane electrical potential by Sucrose-Gap Technique

    Science.gov (United States)

    Yuzhakov, AD; Nosarev, AV; Aleinik, AN

    2017-11-01

    This article describes the development of the experimental setup for measuring the cell membrane electrical potential by Double -Sucrose-Gap Technique. The double-gap isolation method allows the simultaneous measurement of electrical activity and tension output from contracting segments of muscle fibers. This technique has been widely used as a convenient tool for recording of the membrane activities from myelinated or unmyelinated nerves and muscle preparations. This device can be an effective way to provide undergraduate biomedical engineering students with invaluable experiences in neurophysiology. The installation design and its main characteristics are described. The advantages of the described device are the simplicity of the experiment, relatively low cost, the possibility of long-term experiment.

  7. Cell surface topology creates high Ca2+ signalling microdomains

    DEFF Research Database (Denmark)

    Brasen, Jens Christian; Olsen, Lars Folke; Hallett, Maurice B

    2010-01-01

    of a smooth cell surface predicts only moderate localized effects, the more realistic "wrinkled" surface topology predicts that Ca2+ concentrations up to 80 microM can persist within the folds of membranes for significant times. This intra-wrinkle location may account for 5% of the total cell volume. Using...... different geometries of wrinkles, our simulations show that high Ca2+ microdomains will be generated most effectively by long narrow membrane wrinkles of similar dimensions to those found experimentally. This is a new concept which has not previously been considered, but which has ramifications as the intra-wrinkle...

  8. [Cell surface carbohydrates are involved in various biological processes].

    Science.gov (United States)

    Bryne, M; Kjaerheim, A; Schreurs, O; Dabelsteen, E

    1992-09-20

    All human cells show carbohydrate structures on the surface. New knowledge about the genetic mechanisms for the synthesis of these carbohydrates and the generation of monoclonal antibodies with high specificity shows that carbohydrates are involved in various cell-cell and cell-matrix interactions. For instance, cell surface carbohydrates seem to be important in connection with fertilization, embryonic development, cell differentiation, cancer, adhesion of microorganisms and immunological processes. This new knowledge will increase our understanding of various biological phenomena, and will thus be of value for diagnosis and treatment of various diseases.

  9. Soluble and cell surface receptors for tumor necrosis factor

    DEFF Research Database (Denmark)

    Wallach, D; Engelmann, H; Nophar, Y

    1991-01-01

    . The intracellular domains of the two receptors differ in structure, suggesting that they mediate different activities. Their extracellular domains, however, are structurally related. Both contain cysteine-rich repeats which are homologous to repeated structures found in the extracellular domains of the nerve growth...... in certain pathological situations. Release of the soluble receptors from the cells seems to occur by proteolytic cleavage of the cell surface forms and appears to be a way of down-regulating the cell response to TNF. Because of their ability to bind TNF, the soluble receptors exert an inhibitory effect...

  10. Cardiac effects of anabolic steroids: hypertrophy, ischemia and electrical remodelling as potential triggers of sudden death.

    Science.gov (United States)

    Nascimento, J H M; Medei, E

    2011-05-01

    Anabolic-androgenic steroids (AAS) are synthetic testosterone derivatives developed to maximise anabolic activity and minimise androgenic activity. AAS abuse is widespread among both athletes and non-athletes at fitness centres and is becoming a public health issue. In addition to their atherogenic, thrombogenic and spastic effects, AAS have direct cardiotoxic effects by causing hypertrophy, electrical and structural remodelling, and contractile dysfunction and by increasing the susceptibility to ischemic injuries. All of these factors contribute to an increased risk of ventricular arrhythmias and sudden cardiac death.

  11. Potential benefits of long-distance electricity transmission in China for air quality and climate

    Science.gov (United States)

    Peng, W.; Mauzerall, D. L.; Yuan, J.; Zhao, Y.; Lin, M.; Zhang, Q.

    2015-12-01

    China is expanding west-to-east long-distance electricity transmission capacity with the aim of reducing eastern coal power production and resulting air pollution. In addition to coal power, this new grid capacity can be used to transport renewable-generated electricity with resulting climate co-benefits. Here we use an integrated assessment to evaluate the air quality and climate benefits of twelve proposed transmission lines in China, and compare two energy-by-wire strategies that transmit 1) only coal power (Coal-by-wire, CbW) or 2) combined renewable plus coal power (Renewable and coal-by-wire, (RE+C)bW), with 3) the current practice of transporting coal by rail for conversion to electricity near eastern demand centers (Coal-by-rail, CbR). Based on a regional atmospheric chemistry model, WRF-Chem, electricity transmission through the proposed lines leads to 2-3 μg/m3 (2-7%) reduction in the annual mean concentrations of fine particulate matter (PM2.5) in the eastern provinces relative to 2010 levels, roughly ~1 μg/m3 greater than the reduction achieved in CbR where dirty coal units are locally replaced with efficient ones. Although the eastern air quality improvement is similar irrespective of the fuel source to power the lines, adding coal generation results in up to 3% increase in annual mean PM2.5 levels in some exporting provinces, whereas such increase is not observed when most added capacity is renewable-based. Counting both the economic value of reduced carbon emissions and the health-related air quality benefits can significantly improve the cost-effectiveness of transmitting both renewable and coal power. Comparing (RE+C)bW with the two coal-based options, we find not only 20% larger reduction in air-pollution-related deaths, but also three times greater reduction in CO2 emissions. Our study hence demonstrates the significance of coordinating renewable energy planning with transmission planning to simultaneously tackle air pollution and climate

  12. Cell surface hydrophobicity is conveyed by S-layer proteins - A study in recombinant lactobacilli

    NARCIS (Netherlands)

    Mei, H.C. van der; Belt-Gritter, B. van de; Pouwels, P.H.; Martinez, B.; Busscher, H.J.

    2003-01-01

    Cell surface hydrophobicity is one of the most important factors controlling adhesion of microorganisms to surfaces. In this paper, cell surface properties of lactobacilli and recombinant lactobacilli with and without a surface layer protein (SLP) associated with cell surface hydrophobicity were

  13. Cell surface hydrophobicity is conveyed by S-layer proteins - a study in recombinant lactobacilli

    NARCIS (Netherlands)

    van der Mei, HC; van de Belt-Gritter, B; Pouwels, PH; Martinez, B; Busscher, HJ

    2003-01-01

    Cell surface hydrophobicity is one of the most important factors controlling adhesion of microorganisms to surfaces. In this paper, cell surface properties of lactobacilli and recombinant lactobacilli with and without a surface layer protein (SLP) associated with cell surface hydrophobicity were

  14. Assessing the Potential of Plug-in Electric Vehicles in Active Distribution Networks

    Directory of Open Access Journals (Sweden)

    Reza Ahmadi Kordkheili

    2016-01-01

    Full Text Available A multi-objective optimization algorithm is proposed in this paper to increase the penetration level of renewable energy sources (RESs in distribution networks by intelligent management of plug-in electric vehicle (PEV storage. The proposed algorithm is defined to manage the reverse power flow (PF from the distribution network to the upstream electrical system. Furthermore, a charging algorithm is proposed within the proposed optimization in order to assure PEV owner’s quality of service (QoS. The method uses genetic algorithm (GA to increase photovoltaic (PV penetration without jeopardizing PEV owners’ (QoS and grid operating limits, such as voltage level of the grid buses. The method is applied to a part of the Danish low voltage (LV grid to evaluate its effectiveness and capabilities. Different scenarios have been defined and tested using the proposed method. Simulation results demonstrate the capability of the algorithm in increasing solar power penetration in the grid up to 50%, depending on the PEV penetration level and the freedom of the system operator in managing the available PEV storage.

  15. Potential interests and limits of magnetic and electrical stimulation techniques to assess neuromuscular fatigue.

    Science.gov (United States)

    Millet, G Y; Bachasson, D; Temesi, J; Wuyam, B; Féasson, L; Vergès, S; Lévy, P

    2012-12-01

    Neuromuscular function can change under different conditions such as ageing, training/detraining, long-term spaceflight, environmental conditions (e.g. hypoxia, hyperthermia), disease, therapy/retraining programs and also with the appearance of fatigue. Neuromuscular fatigue can be defined as any decrease in maximal voluntary strength or power. There is no standardized method to induce fatigue and various protocols involving different contraction patterns (such as sustained or intermittent submaximal isometric or dynamic contractions on isokinetic or custom chairs) have been used. Probably due to lack of motivation/cooperation, results of fatigue resistance protocols are more variable in patients than in healthy subjects. Magnetic and electrical stimulation techniques allow non-invasive assessment of central and peripheral origins of fatigue. They also allow investigation of different types of muscle fatigue when combining various types of stimulation with force/surface EMG measurements. Since maximal electrical stimuli may be uncomfortable or even sometimes painful, several alternative methods have been recently proposed: submaximal muscle stimulation, low/high-frequency paired pulses instead of tetanic stimuli and the use of magnetic stimulation at the peripheral level. Copyright © 2012 Elsevier B.V. All rights reserved.

  16. Ships hull corrosion diagnosis from close measurements of electric potential in the water

    Science.gov (United States)

    Guibert, A.; Chadebec, O.; Coulomb, J.-L.; Rannou, C.; Nogueira, R. P.

    2010-11-01

    We present here an original application linking an electrochemical domain and the computational aspect of electromagnetic fields to make a corrosion diagnosis of a protected underwater steel structure. After a defined operating time, it is mandatory to check an underwater steel structure. This control may happen too early, if the protection has not been damaged; but it may happen too late, if some particularly bad environmental conditions have already damaged the structure. Moreover, current examination techniques require immobilizing the structure for a long time: they may be complicated to dry (pipe lines or tanks) or to place in dry docks for vessels. The efficiency of these techniques could be improved. The purpose of this paper is to replace this checking/examination by a series of close electrical measurements in the electrolyte which provides a corrosion diagnosis of the structure. This also allows computing the electric and magnetic fields caused by this phenomenon everywhere in the electrolyte. The new method introduced ensures a great time-saving but also an accuracy never reached before.

  17. An Investigation of the Design Potential of Thermochromic Home Textiles Used with Electric Heating Techniques

    Directory of Open Access Journals (Sweden)

    Hung-Jen Chen

    2015-01-01

    Full Text Available Thermochromic colorants have been developed since before the 1900s. There are a large number of patents in different applications of thermochromic textiles, but many innovations leave the field of aesthetic and functional textile design unexplored in the area of smart materials. This study aims to develop thermochromic home textiles that change colors and patterns by integrating thermochromic pigments and electric conductive yarns into textile structures. Stainless steel conductive yarns were sewed on textile substrates to enable heat generation to increase fabric temperature. The heat generation and temperature rise could be controlled by monitoring the voltage applied. The experiments of this study focused on analyzing electric resistance and heating properties of the conductive yarns and observing color changing time and color changing effects of the thermochromic textiles. By using the technique in this research, an image of “tai chi” was designed and implemented in a backlighting thermochromic fabric. It illustrates a range of opportunities for thermochromic textiles in new design research directions of Chinese calligraphy and traditional Chinese painting.

  18. On the detectability of transverse cracks in laminated composites using electrical potential change measurements

    KAUST Repository

    Selvakumaran, Lakshmi

    2015-03-01

    Real-time health monitoring of structures made of laminated composites is necessary as significant damage may occur without any visible signs on the surface. Inspection by electrical tomography (ET) seems a viable approach that relies on voltage measurements from a network of electrodes across the inspected domain to infer conductivity change within the bulk material. If conductivity decreases significantly with increasing damage, the obtained conductivity map can be correlated to the degradation state of the material. We focus here on detection of transverse cracks. As transverse cracks modify the in-plane transverse conductivity of a single ply, we expect them to be detectable by electrical measurements. Yet, the quality of detection is directly related to the sensitivity of the measurements to the presence of cracks. We use numerical experiments to demonstrate that the sensitivity depends on several material and geometrical parameters. Based on the results, the applicability of ET to detect transverse cracks is discussed. One conclusion from the study is that detecting transverse cracks using ET is more reliable in some laminate configurations than in others. Recommendations about the properties of either the pristine material or the inspected structures are provided to establish if ET is reliable in detecting transverse cracks.

  19. Nanoconfined water under electric field at constant chemical potential undergoes electrostriction.

    Science.gov (United States)

    Vanzo, Davide; Bratko, D; Luzar, Alenka

    2014-02-21

    Electric control of nanopore permeation by water and solutions enables gating in membrane ion channels and can be exploited for transient surface tuning of rugged substrates, to regulate capillary permeability in nanofluidics, and to facilitate energy absorption in porous hydrophobic media. Studies of capillary effects, enhanced by miniaturization, present experimental challenges in the nanoscale regime thus making molecular simulations an important complement to direct measurement. In a molecular dynamics (MD) simulation, exchange of water between the pores and environment requires modeling of coexisting confined and bulk phases, with confined water under the field maintaining equilibrium with the unperturbed environment. In the present article, we discuss viable methodologies for MD sampling in the above class of systems, subject to size-constraints and uncertainties of the barostat function under confinement and nonuniform-field effects. Smooth electric field variation is shown to avoid the inconsistencies of MD integration under abruptly varied field and related ambiguities of conventional barostatting in a strongly nonuniform interfacial system. When using a proper representation of the field at the border region of the confined water, we demonstrate a consistent increase in electrostriction as a function of the field strength inside the pore open to a field-free aqueous environment.

  20. Nanoparticle-enhanced electrical impedance detection and its potential significance in image tomography.

    Science.gov (United States)

    Liu, Ran; Jin, Cuiyun; Song, Fengjuan; Liu, Jing

    2013-01-01

    The conductivity and permittivity of tumors are known to differ significantly from those of normal tissues. Electrical impedance tomography (EIT) is a relatively new imaging method for exploiting these differences. However, the accuracy of data capture is one of the difficult problems urgently to be solved in the clinical application of EIT technology. A new concept of EIT sensitizers is put forward in this paper with the goal of expanding the contrast ratio of tumor and healthy tissue to enhance EIT imaging quality. The use of nanoparticles for changing tumor characteristics and determining the infiltration vector for easier detection has been widely accepted in the biomedical field. Ultra-pure water, normal saline, and gold nanoparticles, three kinds of material with large differences in electrical characteristics, are considered as sensitizers and undergo mathematical model analysis and animal experimentation. Our preliminary results suggest that nanoparticles are promising for sensitization work. Furthermore, in experimental and simulation results, we found that we should select different sensitizers for the detection of different types and stages of tumor.

  1. Gastric potential difference measurements. The gastric mucosal integrity and function studied with a new method for measurement of the electric potential difference across the stomach wall

    DEFF Research Database (Denmark)

    Højgaard, L

    1991-01-01

    be reduced by allopurinol pretreatment, possibly due to the inhibition of oxygen-derived free radical formation. Gastric PD and pH were measured in volunteers and duodenal ulcer patients during Stroop's color word conflict test, in which mental stress causes sympathetic activation. A PD reduction and a p......PD--the electric potential difference across the gastric mucosa--is a variable used to describe the gastric mucosal integrity and function. A new, reliable, and easily applied method for gastric PD measurements corrected for the disturbing liquid junction potentials between gastric juice and the PD...... measuring probe is presented. PD is measured with the gastric lumen negative, and a numeric reduction in PD is used as an expression of an injured mucosal condition. A reduced gastric PD is found along with a reduced gastric mucosal blood flow after intravenous indomethacin in anesthetized dogs. Increasing...

  2. Assessing current and future techno-economic potential of concentrated solar power and photovoltaic electricity generation

    NARCIS (Netherlands)

    Köberle, Alexandre C.; Gernaat, David E H J; van Vuuren, Detlef P.

    2015-01-01

    CSP and PV technologies represent energy sources with large potentials. We present cost-supply curves for both technologies using a consistent methodology for 26 regions, based on geoexplicit information on solar radiation, land cover type and slope, exploring individual potential and

  3. Mechanisms of Translocation of ER Chaperones to the Cell Surface and Immunomodulatory Roles in Cancer and Autoimmunity

    Science.gov (United States)

    Wiersma, Valerie R.; Michalak, Marek; Abdullah, Trefa M.; Bremer, Edwin; Eggleton, Paul

    2015-01-01

    Endoplasmic reticulum (ER) chaperones (e.g., calreticulin, heat shock proteins, and isomerases) perform a multitude of functions within the ER. However, many of these chaperones can translocate to the cytosol and eventually the surface of cells, particularly during ER stress induced by e.g., drugs, UV irradiation, and microbial stimuli. Once on the cell surface or in the extracellular space, the ER chaperones can take on immunogenic characteristics, as mostly described in the context of cancer, appearing as damage-associated molecular patterns recognized by the immune system. How ER chaperones relocate to the cell surface and interact with other intracellular proteins appears to influence whether a tumor cell is targeted for cell death. The relocation of ER proteins to the cell surface can be exploited to target cancer cells for elimination by immune mechanism. Here we evaluate the evidence for the different mechanisms of ER protein translocation and binding to the cell surface and how ER protein translocation can act as a signal for cancer cells to undergo killing by immunogenic cell death and other cell death pathways. The release of chaperones can also exacerbate underlying autoimmune conditions, such as rheumatoid arthritis and multiple sclerosis, and the immunomodulatory role of extracellular chaperones as potential cancer immunotherapies requires cautious monitoring, particularly in cancer patients with underlying autoimmune disease. PMID:25688334

  4. Mechanisms of translocation of ER chaperones to the cell surface and immunomodulatory roles in cancer and autoimmunity

    Directory of Open Access Journals (Sweden)

    Valerie R. Wiersma

    2015-01-01

    Full Text Available Endoplasmic reticulum (ER chaperones (e.g. calreticulin, heat shock proteins and isomerases perform a multitude of functions within the ER. However, many of these chaperones can translocate to the cytosol and eventually the surface of cells, particularly during ER stress induced by e.g. drugs, UV irradiation and microbial stimuli. Once on the cell surface or in the extracellular space, the ER chaperones can take on immunogenic characteristics, as mostly described in the context of cancer, appearing as damage-associated molecular patterns recognized by the immune system. How ER chaperones relocate to the cell surface and interact with other intracellular proteins appears to influence whether a tumor cell is targeted for cell death. The relocation of ER proteins to the cell surface can be exploited to target cancer cells for elimination by immune mechanism. Here we evaluate the evidence for the different mechanisms of ER protein translocation and binding to the cell surface and how ER protein translocation can act as a signal for cancer cells to undergo killing by immunogenic cell death and other cell death pathways. The release of chaperones can also exacerbate underlying autoimmune conditions, such as rheumatoid arthritis and multiple sclerosis, and the immunomodulatory role of extracellular chaperones as potential cancer immunotherapies requires cautious monitoring, particularly in cancer patients with underlying autoimmune disease.

  5. Mechanisms of Translocation of ER Chaperones to the Cell Surface and Immunomodulatory Roles in Cancer and Autoimmunity.

    Science.gov (United States)

    Wiersma, Valerie R; Michalak, Marek; Abdullah, Trefa M; Bremer, Edwin; Eggleton, Paul

    2015-01-01

    Endoplasmic reticulum (ER) chaperones (e.g., calreticulin, heat shock proteins, and isomerases) perform a multitude of functions within the ER. However, many of these chaperones can translocate to the cytosol and eventually the surface of cells, particularly during ER stress induced by e.g., drugs, UV irradiation, and microbial stimuli. Once on the cell surface or in the extracellular space, the ER chaperones can take on immunogenic characteristics, as mostly described in the context of cancer, appearing as damage-associated molecular patterns recognized by the immune system. How ER chaperones relocate to the cell surface and interact with other intracellular proteins appears to influence whether a tumor cell is targeted for cell death. The relocation of ER proteins to the cell surface can be exploited to target cancer cells for elimination by immune mechanism. Here we evaluate the evidence for the different mechanisms of ER protein translocation and binding to the cell surface and how ER protein translocation can act as a signal for cancer cells to undergo killing by immunogenic cell death and other cell death pathways. The release of chaperones can also exacerbate underlying autoimmune conditions, such as rheumatoid arthritis and multiple sclerosis, and the immunomodulatory role of extracellular chaperones as potential cancer immunotherapies requires cautious monitoring, particularly in cancer patients with underlying autoimmune disease.

  6. Nonlinear diffusion and thermo-electric coupling in a two-variable model of cardiac action potential

    Science.gov (United States)

    Gizzi, A.; Loppini, A.; Ruiz-Baier, R.; Ippolito, A.; Camassa, A.; La Camera, A.; Emmi, E.; Di Perna, L.; Garofalo, V.; Cherubini, C.; Filippi, S.

    2017-09-01

    This work reports the results of the theoretical investigation of nonlinear dynamics and spiral wave breakup in a generalized two-variable model of cardiac action potential accounting for thermo-electric coupling and diffusion nonlinearities. As customary in excitable media, the common Q10 and Moore factors are used to describe thermo-electric feedback in a 10° range. Motivated by the porous nature of the cardiac tissue, in this study we also propose a nonlinear Fickian flux formulated by Taylor expanding the voltage dependent diffusion coefficient up to quadratic terms. A fine tuning of the diffusive parameters is performed a priori to match the conduction velocity of the equivalent cable model. The resulting combined effects are then studied by numerically simulating different stimulation protocols on a one-dimensional cable. Model features are compared in terms of action potential morphology, restitution curves, frequency spectra, and spatio-temporal phase differences. Two-dimensional long-run simulations are finally performed to characterize spiral breakup during sustained fibrillation at different thermal states. Temperature and nonlinear diffusion effects are found to impact the repolarization phase of the action potential wave with non-monotone patterns and to increase the propensity of arrhythmogenesis.

  7. Methods To Identify Aptamers against Cell Surface Biomarkers

    Directory of Open Access Journals (Sweden)

    Frédéric Ducongé

    2011-09-01

    Full Text Available Aptamers are nucleic acid-based ligands identified through a process of molecular evolution named SELEX (Systematic Evolution of Ligands by Exponential enrichment. During the last 10-15 years, numerous aptamers have been developed specifically against targets present on or associated with the surface of human cells or infectious pathogens such as viruses, bacteria, fungi or parasites. Several of the aptamers have been described as potent probes, rivalling antibodies, for use in flow cytometry or microscopy. Some have also been used as drugs by inhibiting or activating functions of their targets in a manner similar to neutralizing or agonistic antibodies. Additionally, it is straightforward to conjugate aptamers to other agents without losing their affinity and they have successfully been used in vitro and in vivo to deliver drugs, siRNA, nanoparticles or contrast agents to target cells. Hence, aptamers identified against cell surface biomarkers represent a promising class of ligands. This review presents the different strategies of SELEX that have been developed to identify aptamers for cell surface-associated proteins as well as some of the methods that are used to study their binding on living cells.

  8. Semiquantitative determination of circulating islet cell surface antibodies in diabetes

    Energy Technology Data Exchange (ETDEWEB)

    Ohgawara, Hisako; Machiyama, Etsuko; Hirata, Yukimasa (Tokyo Women' s Medical Coll. (Japan))

    1982-09-01

    Circulating pancreatic islet cell antibodies have been demonstrated in patients with insulin-dependent diabetes (IDD). The islet cell surface antibodies (ICSA) were determined by an indirect immunofluorescence test using a suspension of viable islet cells, and similar cytoplasmic antibodies which require the use of group O human pancreas were also found in the serum of some patients. A strong association exists between the presence of islet cell antibodies and the onset of insulin-dependent diabetes. The quantitative determination of circulating ICSA using /sup 125/I-protein A, which binds to IgG attached to the islet cell surface, was essentially as described by Lernmark et al. In the present study, we determined the circulating ICSA in diabetes, especially in IDD. The ICSA were estimated in various sera from both indirect immunofluorescence and /sup 125/I-protein A. Controls bound <2,000 cpm /sup 125/I-protein A. Sera from 4 IDD patients with circulating ICSA demonstrated by immunofluorescence showed >3,000 cpm /sup 125/I-protein A binding activity, and that from 5 patients without ICSA bound <2,000 cpm. Sera from newly-diagnosed diabetics who had severe hyperglycemia showed <2,000 cpm, with or without ICSA.

  9. Spatial patterns and cell surface clusters in perineuronal nets.

    Science.gov (United States)

    Arnst, Nikita; Kuznetsova, Svetlana; Lipachev, Nikita; Shaikhutdinov, Nurislam; Melnikova, Anastasiya; Mavlikeev, Mikhail; Uvarov, Pavel; Baltina, Tatyana V; Rauvala, Heikki; Osin, Yuriy N; Kiyasov, Andrey P; Paveliev, Mikhail

    2016-10-01

    Perineuronal nets (PNN) ensheath GABAergic and glutamatergic synapses on neuronal cell surface in the central nervous system (CNS), have neuroprotective effect in animal models of Alzheimer disease and regulate synaptic plasticity during development and regeneration. Crucial insights were obtained recently concerning molecular composition and physiological importance of PNN but the microstructure of the network remains largely unstudied. Here we used histochemistry, fluorescent microscopy and quantitative image analysis to study the PNN structure in adult mouse and rat neurons from layers IV and VI of the somatosensory cortex. Vast majority of meshes have quadrangle, pentagon or hexagon shape with mean mesh area of 1.29µm(2) in mouse and 1.44µm(2) in rat neurons. We demonstrate two distinct patterns of chondroitin sulfate distribution within a single mesh - with uniform (nonpolar) and node-enriched (polar) distribution of the Wisteria floribunda agglutinin-positive signal. Vertices of the node-enriched pattern match better with local maxima of chondroitin sulfate density as compared to the uniform pattern. PNN is organized into clusters of meshes with distinct morphologies on the neuronal cell surface. Our findings suggest the role for the PNN microstructure in the synaptic transduction and plasticity. Copyright © 2016 Elsevier B.V. All rights reserved.

  10. Immunomic Screening of Cell Surface Molecules on Undifferentiated Human Dental Pulp Stem Cells.

    Science.gov (United States)

    Hwang, Hyo-In; Lee, Tae-Hyung; Kang, Kyung-Jung; Ryu, Chun-Jeih; Jang, Young-Joo

    2015-08-15

    Human adult dental pulp tissue is a source of adult stem cells that have a potential to differentiate into various tissues, although the primary cell suspensions cultured from pulp tissue are mixtures of both stem cell and nonstem cell populations with heterogeneous phenotypes and various differentiation efficiencies. Therefore, cell surface protein markers on dental pulp stem cells are critical for detection and purification of stem cell populations. Yet, little is known about the cell surface molecules that are specifically associated with the undifferentiated and progenitor state of human adult dental pulp stem cells (hDPSCs). Presently, cell surface proteins expressed on hDPSCs were assessed by screening surface molecules specifically expressed on dentinogenic progenitors. Using a decoy immunization strategy, a set of monoclonal antibodies (MAbs) was generated against undifferentiated pulp progenitor cells. Forty-five hybridomas produced MAbs that interacted weakly, if at all, to differentiated pulp cells. Of these, 19 MAbs (18 IgG, 1 IgM) recognized surface molecules on undifferentiated hDPSCs. By multicolor flow cytometric analysis, 40%-60% of newly identified MAb-positive cells were demonstrated to be positive for the CD44 and CD90 mesenchymal markers. When MAb-positive cells were sorted from the heterogeneous pulp cell suspension, mineralization efficiency was increased three to five times compared with MAb-negative cells. The results suggest that the decoy immunization is an efficient method for isolation of MAbs against dentinogenic progenitors. These MAbs will be helpful for identification and enrichment of hDPSCs for efficient dentin regeneration.

  11. Effects of altered sialic acid biosynthesis on N-linked glycan branching and cell surface interactions.

    Science.gov (United States)

    Pham, Nam D; Pang, Poh-Choo; Krishnamurthy, Soumya; Wands, Amberlyn M; Grassi, Paola; Dell, Anne; Haslam, Stuart M; Kohler, Jennifer J

    2017-06-09

    GNE (UDP-GlcNAc 2-epimerase/ManNAc kinase) myopathy is a rare muscle disorder associated with aging and is related to sporadic inclusion body myositis, the most common acquired muscle disease of aging. Although the cause of sporadic inclusion body myositis is unknown, GNE myopathy is associated with mutations in GNE. GNE harbors two enzymatic activities required for biosynthesis of sialic acid in mammalian cells. Mutations to both GNE domains are linked to GNE myopathy. However, correlation between mutation-associated reductions in sialic acid production and disease severity is imperfect. To investigate other potential effects of GNE mutations, we compared sialic acid production in cell lines expressing wild type or mutant forms of GNE. Although we did not detect any differences attributable to disease-associated mutations, lectin binding and mass spectrometry analysis revealed that GNE deficiency is associated with unanticipated effects on the structure of cell-surface glycans. In addition to exhibiting low levels of sialylation, GNE-deficient cells produced distinct N-linked glycan structures with increased branching and extended poly-N-acetyllactosamine. GNE deficiency may affect levels of UDP-GlcNAc, a key metabolite in the nutrient-sensing hexosamine biosynthetic pathway, but this modest effect did not fully account for the change in N-linked glycan structure. Furthermore, GNE deficiency and glucose supplementation acted independently and additively to increase N-linked glycan branching. Notably, N-linked glycans produced by GNE-deficient cells displayed enhanced binding to galectin-1, indicating that changes in GNE activity can alter affinity of cell-surface glycoproteins for the galectin lattice. These findings suggest an unanticipated mechanism by which GNE activity might affect signaling through cell-surface receptors. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  12. Effect of Set Potential on Hexavalent Chromium Reduction and Electricity Generation from Biocathode Microbial Fuel Cells

    KAUST Repository

    Huang, Liping

    2011-06-01

    Setting a biocathode potential at ?300 mV improved the subsequent performance of an MFC for Cr(VI) reduction compared to a control (no set potential). With this set potential, the startup time was reduced to 19 days, the reduction of Cr(VI) was improved to 19.7 mg/L d, and the maximum power density was increased to 6.4 W/m3 compared to the control (26 days, 14.0 mg/L d and 4.1 W/m3). Set potentials of ?150 mV and ?300 mV also improved system performance and led to similarly higher utilization of metabolic energy gained (PMEG) than set potentials of +200 mV and ?450 mV. We observed putative pili at ?150 and ?300 mV potentials, and aggregated precipitates on bacterial surfaces in both poised and nonpoised controls. These tests show that there are optimal potentials that can be set for developing a Cr(VI) biocathode. © 2011 American Chemical Society.

  13. Coarse-grained simulations for organic molecular liquids based on Gay-Berne and electric multipole potentials.

    Science.gov (United States)

    Xu, Peijun; Shen, Hujun; Yang, Lu; Ding, Yang; Li, Beibei; Shao, Ying; Mao, Yingchen; Li, Guohui

    2013-02-01

    Coarse-grained studies of CH(3)SH, CH(3)CHO and CHCl(3) liquids, based on anisotropic Gay-Berne (GB) and electric multipole potentials (EMP), demonstrate that the coarse-grained model is able to qualitatively reproduce the results obtained from the atomistic model (AMOEBA polarizable force field) and allows for significant saving in computation time. It should be pointed out that the accuracy of the coarse-grained model is very sensitive to how well the anisotropic GB particle is defined and how satisfactorily the EMP sites are chosen.

  14. Numerical Modeling of the Electric Field and the Potential Distributions in Heterogeneous Cavities inside XLPE Power Cable Insulation

    Directory of Open Access Journals (Sweden)

    KHOUILDI Emna

    2016-10-01

    Full Text Available The XLPE power cable is easily affected by heterogeneous cavities: air void cavity, vented and bow-tie water trees. In this paper, we present a numerical modeling of this cable. A number of simulations are realized in order to analyze the influence of heterogeneous cavities and the influence of their positions and sizes on the electric field distribution, the potential distribution and the degradation of the XLPE insulation. The models are implemented with the finite element method based on the software package COMSOL Multiphysics.

  15. About the Territorial Potential of the Construction of Battery-Charging Stations for Autonomous Electric Motor Vehicles in the Regions

    Directory of Open Access Journals (Sweden)

    Shilova Lyubov

    2016-01-01

    Full Text Available The article describes the main current trends in the development of electric motor vehicles with "zero emission" as well as the battery-charging stations concerned. The study is based on a preliminary comparative analysis of the RF regions with respect to five indices (average per capita income, number of private cars in the region, air pollution level, provision of the region with power supply and the potential use of local renewable energy resources, and it gives some recommendations on the prospects of possible construction of battery-charging stations in the regions.

  16. The potential of agro-industrial residues and municipal solid waste for production of biogas and electricity in Tanzania

    Energy Technology Data Exchange (ETDEWEB)

    Kivaisi, A.K. [Univ. of Dar es Salaam, Botany Dept., Applied Microbiology Unit (Tanzania, United Republic of)

    1997-12-31

    This paper gives an overview of the energy demands in Tanzania, and highlights the current serious shortage of electricity. Government strategy to alleviate the problem include exploitation of the country`s big natural gas reserves for power generation, and utilization of the renewable energies such as solar, wind and biogas. Important agro-industrial residues and municipal solid wastes with large potentials for anaerobic converstion into biogas and electricity have been identified and quantified. Tanzania is estimated to generate about 615,000 organic matter from coffee, sisal, sugar and cereal residues and households in main towns are estimated to generate about 600,000 tons of organic matter annually. Laboratory scale determinations of methane yields from the residues gave 400 m{sup 3} CH{sub 4}/ton VS of sisal pulp; 400 m{sup 3} CH{sub 4}/ton VS of sisal production wastewater; 400 m{sup 3} CH{sub 4}/ton VS of Robusta coffee solid waste, 350 m{sup 3} CH{sub 4}/ton VS of sugar processing wastewater; 250 m{sup 3} CH{sub 4}/ton VS of sugar filter mat, 450 m{sup 3} CH{sub 4}/ton VS maize bran and 300 m{sup 3} CH{sub 4}/ton VS of mixed household waste. Based on these results the estimated total annual potential electricity production from these residues is 1.4 million MW. The total oil substitution from these residues has been estimated at 0.35 million tonnes crude diesel oil per annum equivalent to 2% of the total energy consumption in Tanzania. Case studies onthe coffee and sisal processing factories indicate that exploitation of the residues for the production of electricity on site these factories is feasible. Utilization of agro-industrial residues and municipal waste for biogas production has enormous potential for reduction of environmental pollution. The potential substitution of fossil fuel with biogas represents an annual reduction in the net CO{sub 2} emission to the atmosphere of approximately 1.3 million tonnes. By treating the residues in controlled

  17. More homogeneous wind conditions under strong climate change decrease the potential for inter-state balancing of electricity in Europe

    Science.gov (United States)

    Wohland, Jan; Reyers, Mark; Weber, Juliane; Witthaut, Dirk

    2017-11-01

    Limiting anthropogenic climate change requires the fast decarbonization of the electricity system. Renewable electricity generation is determined by the weather and is hence subject to climate change. We simulate the operation of a coarse-scale fully renewable European electricity system based on downscaled high-resolution climate data from EURO-CORDEX. Following a high-emission pathway (RCP8.5), we find a robust but modest increase (up to 7 %) of backup energy in Europe through the end of the 21st century. The absolute increase in the backup energy is almost independent of potential grid expansion, leading to the paradoxical effect that relative impacts of climate change increase in a highly interconnected European system. The increase is rooted in more homogeneous wind conditions over Europe resulting in intensified simultaneous generation shortfalls. Individual country contributions to European generation shortfall increase by up to 9 TWh yr-1, reflecting an increase of up to 4 %. Our results are strengthened by comparison with a large CMIP5 ensemble using an approach based on circulation weather types.

  18. More homogeneous wind conditions under strong climate change decrease the potential for inter-state balancing of electricity in Europe

    Directory of Open Access Journals (Sweden)

    J. Wohland

    2017-11-01

    Full Text Available Limiting anthropogenic climate change requires the fast decarbonization of the electricity system. Renewable electricity generation is determined by the weather and is hence subject to climate change. We simulate the operation of a coarse-scale fully renewable European electricity system based on downscaled high-resolution climate data from EURO-CORDEX. Following a high-emission pathway (RCP8.5, we find a robust but modest increase (up to 7 % of backup energy in Europe through the end of the 21st century. The absolute increase in the backup energy is almost independent of potential grid expansion, leading to the paradoxical effect that relative impacts of climate change increase in a highly interconnected European system. The increase is rooted in more homogeneous wind conditions over Europe resulting in intensified simultaneous generation shortfalls. Individual country contributions to European generation shortfall increase by up to 9 TWh yr−1, reflecting an increase of up to 4 %. Our results are strengthened by comparison with a large CMIP5 ensemble using an approach based on circulation weather types.

  19. Four-point probe measurements using current probes with voltage feedback to measure electric potentials

    Science.gov (United States)

    Lüpke, Felix; Cuma, David; Korte, Stefan; Cherepanov, Vasily; Voigtländer, Bert

    2018-02-01

    We present a four-point probe resistance measurement technique which uses four equivalent current measuring units, resulting in minimal hardware requirements and corresponding sources of noise. Local sample potentials are measured by a software feedback loop which adjusts the corresponding tip voltage such that no current flows to the sample. The resulting tip voltage is then equivalent to the sample potential at the tip position. We implement this measurement method into a multi-tip scanning tunneling microscope setup such that potentials can also be measured in tunneling contact, allowing in principle truly non-invasive four-probe measurements. The resulting measurement capabilities are demonstrated for \

  20. Dipole and quadrupole synthesis of electric potential fields. M.S. Thesis

    Science.gov (United States)

    Tilley, D. G.

    1979-01-01

    A general technique for expanding an unknown potential field in terms of a linear summation of weighted dipole or quadrupole fields is described. Computational methods were developed for the iterative addition of dipole fields. Various solution potentials were compared inside the boundary with a more precise calculation of the potential to derive optimal schemes for locating the singularities of the dipole fields. Then, the problem of determining solutions to Laplace's equation on an unbounded domain as constrained by pertinent electron trajectory data was considered.

  1. Preliminary evaluation of potential NO/sub x/ control strategies for the electric power industry. Volume II. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Greenfield, S.M.; Peyton, T.O.; Anderson, G.E.; Attaway, L.D.; Hillyer, M.J.; MacBride, D.W.

    1978-03-01

    Eight appendices of basic supporting information for the report on Preliminary Evaluation of Potential NO/sub x/ Control Strategies for the Electric Power Industry are presented. The first appendix presents a collection of federal, state and selected local limitations on the emissions and ambient concentration of NO/sub 2/, and a short summary of the potential for near-term changes in these limitations. It is intended to provide a dynamic summary of these regulatory constraints on the electric power industry, a basis for the strategies examined in the current report, and a starting point for the analysis envisioned for the following phase.The second appendix reviews NO/sub x/ control technologies: The third provides emission data for Air Quality Control Regions (AQCR). The remaining appendices contain information on: models used for projecting NO/sub x/ emissions and concentrations for the subject AQCRs; and basic power plants data, current and proposed, for the USA and for the AQCRs examined. (LCL)

  2. Proposal for inclusion of topics of particle physics integrated electric charge through a potentially meaningful teaching units

    Directory of Open Access Journals (Sweden)

    Lisiane Barcellos Calheiro

    2014-03-01

    Full Text Available In this article the results of the analysis of free and concept maps produced are presented from the application and evaluation of a Potentially Meaningful Teaching Units – PMTU, which is a teaching sequence based on various learning theories and seeks to promote meaningful student learning. Presents, in this work, part of a research Masters in Science Education which deals with the inclusion of topics of particle physics integrated with traditional content of the third year of high school. It was implemented in a third grade high school class of a State School in Santa Maria, Rio Grande do Sul, and Brazil. The PMTU aimed to address in an integrated manner threads for Particle Physics and Electronics. A didactic sequence that integrated the topics of electric charge, atomic models, elementary particles, quantization and process electrification was applied. Such integration aimed at stimulating the interest on topics related to Modern and Contemporary Physics. It was developed using PMTU activities that aimed at promoting meaningful learning and knowledge construction in the classroom, Since the topics involved were quite complex, this made their integration a real challenge to the high school teachers, and resulted in changes in their teaching practices. Research showed that the inclusion of topics on physics of elementary particles the and electricity, through Potentially Meaningful Teaching Units, show satisfactory results in the students’ learning.

  3. The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation.

    Science.gov (United States)

    Gundry, Rebekah L; Raginski, Kimberly; Tarasova, Yelena; Tchernyshyov, Irina; Bausch-Fluck, Damaris; Elliott, Steven T; Boheler, Kenneth R; Van Eyk, Jennifer E; Wollscheid, Bernd

    2009-11-01

    Endogenous regeneration and repair mechanisms are responsible for replacing dead and damaged cells to maintain or enhance tissue and organ function, and one of the best examples of endogenous repair mechanisms involves skeletal muscle. Although the molecular mechanisms that regulate the differentiation of satellite cells and myoblasts toward myofibers are not fully understood, cell surface proteins that sense and respond to their environment play an important role. The cell surface capturing technology was used here to uncover the cell surface N-linked glycoprotein subproteome of myoblasts and to identify potential markers of myoblast differentiation. 128 bona fide cell surface-exposed N-linked glycoproteins, including 117 transmembrane, four glycosylphosphatidylinositol-anchored, five extracellular matrix, and two membrane-associated proteins were identified from mouse C2C12 myoblasts. The data set revealed 36 cluster of differentiation-annotated proteins and confirmed the occupancy for 235 N-linked glycosylation sites. The identification of the N-glycosylation sites on the extracellular domain of the proteins allowed for the determination of the orientation of the identified proteins within the plasma membrane. One glycoprotein transmembrane orientation was found to be inconsistent with Swiss-Prot annotations, whereas ambiguous annotations for 14 other proteins were resolved. Several of the identified N-linked glycoproteins, including aquaporin-1 and beta-sarcoglycan, were found in validation experiments to change in overall abundance as the myoblasts differentiate toward myotubes. Therefore, the strategy and data presented shed new light on the complexity of the myoblast cell surface subproteome and reveal new targets for the clinically important characterization of cell intermediates during myoblast differentiation into myotubes.

  4. Longitudinal microarray analysis of cell surface antigens on peripheral blood mononuclear cells from HIV+ individuals on highly active antiretroviral therapy

    Directory of Open Access Journals (Sweden)

    Wang Bin

    2008-03-01

    Full Text Available Abstract Background The efficacy of highly active antiretroviral therapy (HAART determined by simultaneous monitoring over 100 cell-surface antigens overtime has not been attempted. We used an antibody microarray to analyze changes in the expression of 135 different cell-surface antigens overtime on PBMC from HIV+ patients on HAART. Two groups were chosen, one (n = 6 achieved sustainable response by maintaining below detectable plasma viremia and the other (n = 6 responded intermittently. Blood samples were collected over an average of 3 years and 5–8 time points were selected for microarray assay and statistical analysis. Results Significant trends over time were observed for the expression of 7 cell surface antigens (CD2, CD3epsilon, CD5, CD95, CD36, CD27 and CD28 for combined patient groups. Between groups, expression levels of 10 cell surface antigens (CD11a, CD29, CD38, CD45RO, CD52, CD56, CD57, CD62E, CD64 and CD33 were found to be differential. Expression levels of CD9, CD11a, CD27, CD28 and CD52, CD44, CD49d, CD49e, CD11c strongly correlated with CD4+ and CD8+ T cell counts, respectively. Conclusion Our findings not only detected markers that may have potential prognostic/diagnostic values in evaluating HAART efficacy, but also showed how density of cell surface antigens could be efficiently exploited in an array-like manner in relation to HAART and HIV-infection. The antigens identified in this study should be further investigated by other methods such as flow cytometry for confirmation as biological analysis of these antigens may help further clarify their role during HAART and HIV infection.

  5. Greenhouse gas mitigation potential of short-rotation-coppice based generation of electricity in Germany

    Energy Technology Data Exchange (ETDEWEB)

    Hansen, A.; Meyer-Aurich, A.; Kern, J.; Balasus, A.; Prochnow, A. [Leibniz Inst. of Agricultural Engineering, Potsdam (Germany)

    2010-07-01

    The generation of energy from wood biomass may help secure local energy supplies and reduce the greenhouse effect by substituting fossil resources with bio-based ones. In the case of short rotation coppice (SRC), bio-based resources can be generated by extensive agricultural production systems. They produce less carbon dioxide equivalent (CO{sub 2eq}) emissions than fossil resources. This paper reported on a study in which a model system was developed for a regional supply chain producing second generation bioenergy generated from SRC in eastern Germany. The study focuses on the generation of electricity and was compared to a business-as-usual reference system, based on the latest CO{sub 2} mitigation factors for renewable energies in the German power-generation mix. A life cycle assessment based on greenhouse gas (GHG) inventories was also conducted in which other factors were also considered, such as options for nutrient cycling. The key determinants for GHG mitigation with SRC were also discussed with regards to indirect land-use effects resulting from increased demand for land.

  6. Potential production of electricity from biogas generated in a sanitary landfill

    Directory of Open Access Journals (Sweden)

    Quetzalli Aguilar-Virgen

    2011-09-01

    Full Text Available Energy forms the cornerstone of almost every economic, social and cultural sector in modern societies. Energy is regarded as an irreplaceable ingredient in such societies’ industrial development. The aim of this research was to estimate the generation of biogas in the city of Ensenada’s sanitary landfill to ascertain the amount of energy which could be generated from the solid waste being disposed of. Biogas estimates were conducted in two stages: a waste characterisation study followed by implementing the regulations proposed by SCS Engineers (SCS Engineers, 2009 regarding the Mexican biogas model (version 2.0. The results showed that a large quantity of organic matter (around 70% is a key element in anaerobic degradation of waste. As to energy generation, it is believed that a full 1.90 MW capacity will be reached in 2019. Such energy could increase Ensenada’s current electricity generation capacity by 3.46% and provide 60% of the energy needed for street lighting, thereby leading to USD $1.423 million in savings.

  7. Design, Fabrication, and Testing of Emissive Probes to Determine the Plasma Potential of the Plumes of Various Electric Thrusters

    Science.gov (United States)

    Chen, Erinna M.

    2005-01-01

    A significant problem in the use of electric thrusters in spacecraft is the formation of low-energy ions in the thruster plume. Low-energy ions are formed in the plume via random collisions between high-velocity ions ejected from the thruster and slow-moving neutral atoms of propellant effusing from the engine. The sputtering of spacecraft materials due to interactions with low-energy ions may result in erosion or contamination of the spacecraft. The trajectory of these ions is determined primarily by the plasma potential of the plume. Thus, accurate characterization of the plasma potential is essential to predicting low-energy ion contamination. Emissive probes were utilized to determine the plasma potential. When the ion and electron currents to the probe are balanced, the potential of such probes float to the plasma potential. Two emissive probes were fabricated; one utilizing a DC power supply, another utilizing a rectified AC power source. Labview programs were written to coordinate and automate probe motion in the thruster plume. Employing handshaking interaction, these motion programs were synchronized to various data acquisition programs to ensure precision and accuracy of the measurements. Comparing these experimental values to values from theoretical models will allow for a more accurate prediction of low-energy ion interaction.

  8. Multigrid solution of the potential field in modeling electrical nerve stimulation

    NARCIS (Netherlands)

    Hoekema, R.; Hoekema, Rudi; Venner, Cornelis H.; Struijk, J.J.; Struijk, Johannes J.; Holsheimer, J.

    1998-01-01

    In this paper, multilevel techniques are introduced as a fast numerical method to compute 3-D potential field in nerve stimulation configurations. It is shown that with these techniques the computing time is reduced significantly compared to conventional methods. Consequently, these techniques

  9. Detection of Potential Induced Degradation in c-Si PV Panels Using Electrical Impedance Spectroscopy

    DEFF Research Database (Denmark)

    Oprea, Matei-lon; Spataru, Sergiu; Sera, Dezso

    This work, for the first time, investigates an Impedance Spectroscopy (IS) based method for detecting potential-induced degradation (PID) in crystalline silicon photovoltaic (c-Si PV) panels. The method has been experimentally tested on a set of panels that were confirmed to be affected by PID...

  10. Vaccines based on the cell surface carbohydrates of pathogenic bacteria

    Directory of Open Access Journals (Sweden)

    Jones Christopher

    2005-01-01

    Full Text Available Glycoconjugate vaccines, in which a cell surface carbohydrate from a micro-organism is covalently attached to an appropriate carrier protein are proving to be the most effective means to generate protective immune responses to prevent a wide range of diseases. The technology appears to be generic and applicable to a wide range of pathogens, as long as antibodies against surface carbohydrates help protect against infection. Three such vaccines, against Haemophilus influenzae type b, Neisseria meningitidis Group C and seven serotypes of Streptococcus pneumoniae, have already been licensed and many others are in development. This article discusses the rationale for the development and use of glycoconjugate vaccines, the mechanisms by which they elicit T cell-dependent immune responses and the implications of this for vaccine development, the role of physicochemical methods in the characterisation and quality control of these vaccines, and the novel products which are under development.

  11. Mechanotransduction across the cell surface and through the cytoskeleton

    Science.gov (United States)

    Wang, N.; Butler, J. P.; Ingber, D. E.

    1993-01-01

    Mechanical stresses were applied directly to cell surface receptors with a magnetic twisting device. The extracellular matrix receptor, integrin beta 1, induced focal adhesion formation and supported a force-dependent stiffening response, whereas nonadhesion receptors did not. The cytoskeletal stiffness (ratio of stress to strain) increased in direct proportion to the applied stress and required intact microtubules and intermediate filaments as well as microfilaments. Tensegrity models that incorporate mechanically interdependent struts and strings that reorient globally in response to a localized stress mimicked this response. These results suggest that integrins act as mechanoreceptors and transmit mechanical signals to the cytoskeleton. Mechanotransduction, in turn, may be mediated simultaneously at multiple locations inside the cell through force-induced rearrangements within a tensionally integrated cytoskeleton.

  12. Cell surface carbohydrates as prognostic markers in human carcinomas

    DEFF Research Database (Denmark)

    Dabelsteen, Erik

    1996-01-01

    Tumour development is usually associated with changes in cell surface carbohydrates. These are often divided into changes related to terminal carbohydrate structures, which include incomplete synthesis and modification of normally existing carbohydrates, and changes in the carbohydrate core...... structure. The latter includes chain elongation of both glycolipids and proteins, increased branching of carbohydrates in N-linked glycoproteins, and blocked synthesis of carbohydrates in O-linked mucin-like glycoproteins. In mature organisms, expression of distinct carbohydrates is restricted to specific...... cell types; within a given tissue, variation in expression may be related to cell maturation. Tumour-associated carbohydrate structures often reflect a certain stage of cellular development; most of these moieties are structures normally found in other adult or embryonic tissues. There is no unique...

  13. Substrate recognition by the cell surface palmitoyl transferase DHHC5.

    Science.gov (United States)

    Howie, Jacqueline; Reilly, Louise; Fraser, Niall J; Vlachaki Walker, Julia M; Wypijewski, Krzysztof J; Ashford, Michael L J; Calaghan, Sarah C; McClafferty, Heather; Tian, Lijun; Shipston, Michael J; Boguslavskyi, Andrii; Shattock, Michael J; Fuller, William

    2014-12-09

    The cardiac phosphoprotein phospholemman (PLM) regulates the cardiac sodium pump, activating the pump when phosphorylated and inhibiting it when palmitoylated. Protein palmitoylation, the reversible attachment of a 16 carbon fatty acid to a cysteine thiol, is catalyzed by the Asp-His-His-Cys (DHHC) motif-containing palmitoyl acyltransferases. The cell surface palmitoyl acyltransferase DHHC5 regulates a growing number of cellular processes, but relatively few DHHC5 substrates have been identified to date. We examined the expression of DHHC isoforms in ventricular muscle and report that DHHC5 is among the most abundantly expressed DHHCs in the heart and localizes to caveolin-enriched cell surface microdomains. DHHC5 coimmunoprecipitates with PLM in ventricular myocytes and transiently transfected cells. Overexpression and silencing experiments indicate that DHHC5 palmitoylates PLM at two juxtamembrane cysteines, C40 and C42, although C40 is the principal palmitoylation site. PLM interaction with and palmitoylation by DHHC5 is independent of the DHHC5 PSD-95/Discs-large/ZO-1 homology (PDZ) binding motif, but requires a ∼ 120 amino acid region of the DHHC5 intracellular C-tail immediately after the fourth transmembrane domain. PLM C42A but not PLM C40A inhibits the Na pump, indicating PLM palmitoylation at C40 but not C42 is required for PLM-mediated inhibition of pump activity. In conclusion, we demonstrate an enzyme-substrate relationship for DHHC5 and PLM and describe a means of substrate recruitment not hitherto described for this acyltransferase. We propose that PLM palmitoylation by DHHC5 promotes phospholipid interactions that inhibit the Na pump.

  14. Geothermal Potential of the Cascade and Aleutian Arcs, with Ranking of Individual Volcanic Centers for their Potential to Host Electricity-Grade Reservoirs

    Energy Technology Data Exchange (ETDEWEB)

    Shevenell, Lisa [ATLAS Geosciences, Inc., Reno, NV (United States); Coolbaugh, Mark [ATLAS Geosciences, Inc., Reno, NV (United States); Hinz, Nick [Univ. of Nevada, Reno, NV (United States); Stelling, Pete [Western Washington Univ., Bellingham, WA (United States); Melosh, Glenn [GEODE, Santa Rosa, CA (United States); Cumming, William [Cumming Geoscience, Santa Rosa, CA (United States)

    2015-10-16

    This project brings a global perspective to volcanic arc geothermal play fairway analysis by developing statistics for the occurrence of geothermal reservoirs and their geoscience context worldwide in order to rank U.S. prospects. The focus of the work was to develop play fairways for the Cascade and Aleutian arcs to rank the individual volcanic centers in these arcs by their potential to host electricity grade geothermal systems. The Fairway models were developed by describing key geologic factors expected to be indicative of productive geothermal systems in a global training set, which includes 74 volcanic centers world-wide with current power production. To our knowledge, this is the most robust geothermal benchmark training set for magmatic systems to date that will be made public.

  15. Comparison of electrically evoked cortical potential thresholds generated with subretinal or suprachoroidal placement of a microelectrode array in the rabbit

    Science.gov (United States)

    Yamauchi, Yasuyuki; Franco, Luisa M.; Jackson, Douglas J.; Naber, John F.; Ofer Ziv, R.; Rizzo, Joseph F., III; Kaplan, Henry J.; Enzmann, Volker

    2005-03-01

    The aim of the study was to directly compare the threshold electrical charge density of the retina (retinal threshold) in rabbits for the generation of electrical evoked potentials (EEP) by delivering electrical stimulation with a custom-made microelectrode array (MEA) implanted into either the subretinal or suprachoroidal space. Nine eyes of seven Dutch-belted rabbits were studied. The electroretinogram (ERG), visual evoked potentials (VEP) and EEP were recorded. Electrodes for the VEP and EEP were placed on the dura mater overlying the visual cortex. The EEP was recorded following electrical stimulation of the MEA placed either subretinally beneath the visual streak of the retina or in the suprachoroidal space in the rabbit eye. An ab externo approach was used for placement of the MEA. Liquid perfluorodecaline (PFCL; 0.4 ml) was placed within the vitreous cavity to flatten the neurosensory retina on the MEA after subretinal implantation. The retinal threshold for generation of an EEP was determined for each MEA placement by three consecutive measurements consisting of 100 computer-averaged recordings. Animals were sacrificed at the conclusion of the experiment and the eyes were enucleated for histological examination. The retinal threshold to generate an EEP was 9 ± 7 nC (0.023 ± 0.016 mC cm-2) within the subretinal space and 150 ± 122 nC (0.375 ± 0.306 mC cm-2) within the suprachoroidal space. Histology showed disruption of the outer retina with subretinal but not suprachoroidal placement. The retinal threshold to elicit an EEP is significantly lower with subretinal placement of the MEA compared to suprachoroidal placement (P < 0.05). The retinal threshold charge density with a subretinal MEA is well below the published charge limit of 1 mC cm-2, which is the level below which chronic stimulation of the retina is considered necessary to avoid tissue damage (Shannon 1992 IEEE Trans. Biomed. Eng. 39 424-6). Supported in part by The Charles D Kelman, MD

  16. Contamination, potential bias and humidity effects on electrical performance and corrosion reliability of electronic devices

    DEFF Research Database (Denmark)

    Piotrowska, Kamila; Verdingovas, Vadimas; Jellesen, Morten S.

    2015-01-01

    This paper describes the interactions between flux contamination, humidity and po-tential bias and their effect on corrosion reliability. Water layer formation on laminate surfaces and behavior of different solder flux chemistries with humid conditions have been studied, together......-sponse of electronics is estimated as a function of contamination type under cycling humid conditions. The results show the correlation between the ionic contamination type and water layer formation on surfaces in the presence of the flux residue....

  17. Ennoblement Due to Biofilms: Indicator for Potential Corrosion and Source of Electrical Energy

    Science.gov (United States)

    2013-01-01

    conclusion was challenged by Chandrasekaran and Dexter [19] who suggested that Epit for a stainless steel covered by a biofilm might be different from that...presence of a biofilm dramatically increased the current density required to polarize stainless steel to a potential of -400mV (vs. saturated calomel...available on-line monitoring tech- nique [38] and device [39] for biofilm development uses a probe made up of a series of identical stainless steel discs

  18. Simulation of Electric Potentials and Ion Motion in Planar Electrode Structures for Lossless Ion Manipulations (SLIM)

    OpenAIRE

    Garimella, Sandilya V.B.; Ibrahim, Yehia M.; Webb, Ian K.; Tolmachev, Aleksey V.; Zhang, Xinyu; Prost, Spencer A; Anderson, Gordon A.; Smith, Richard D.

    2014-01-01

    We report a conceptual study and computational evaluation of novel planar electrode Structures for Lossless Ion Manipulations (SLIM). Planar electrode SLIM devices were designed that allow for flexible ion confinement, transport and storage using a combination of RF and DC fields. Effective potentials can be generated that provide near ideal regions for confining and manipulating ions in the presence of a gas. Ion trajectory simulations using SIMION 8.1 demonstrated the capability for lossles...

  19. A comparison of emissive probe techniques for electric potential measurements in a complex plasma

    Energy Technology Data Exchange (ETDEWEB)

    Sheehan, J. P.; Hershkowitz, N. [Department of Engineering Physics, University of Wisconsin, Madison, Madison, Wisconsin 53706 (United States); Raitses, Y.; Kaganovich, I.; Fisch, N. J. [Princeton Plasma Physics Laboratory, P.O. Box 451, Princeton, New Jersey 08543-0451 (United States)

    2011-07-15

    The major emissive probe techniques are compared to better understand the floating potential of an electron emitting surface in a plasma. An overview of the separation point technique, floating point technique, and inflection point in the limit of zero emission technique is given, addressing how each method works as well as the theoretical basis and limitations of each. It is shown that while the floating point method is the most popular, it is expected to yield a value {approx}1.5T{sub e}/e below the plasma potential due to a virtual cathode forming around the probe. The theoretical predictions were checked with experiments performed in a 2 kW annular Hall thruster plasma (n{sub e} {approx} 10{sup 9}-10{sup 10} cm{sup -3}and T{sub e} {approx} 10-50 eV). The authors find that the floating point method gives a value around 2T{sub e}/e below the inflection point method, which is shown to be a more accurate emissive probe technique than other techniques used in this work for measurements of the plasma potential.

  20. Use of PCB blood levels to assess potential exposure following an electrical transformer explosion.

    Science.gov (United States)

    Wolff, M S; Schecter, A

    1992-11-01

    Following the explosion of a transformer, passersby, building occupants, and cleanup personnel had potential exposure to the transformer dielectric fluid containing polychlorinated biphenyls (PCBs). As part of a medical evaluation, blood serum was analyzed for PCBs, and the concentrations found were similar to that of a regional comparison group (median 4.0 ng/mL or parts per billion, range 1-10, n = 60). Some workers employed by the utility company that owned the transformer had potential exposure to PCBs in the past. This positive comparison group had significantly higher serum PCB concentrations, related to known direct contact (median 5.0, mean 14 ng/mL, 1-187, n = 25) or not (median 4.0, mean 11 ng/mL, 2-72, n = 17). Therefore, in this investigation, elevation of serum PCB levels could be related to past contact during work with transformers, but not to potential short-term exposure at the time of a transfer explosion.

  1. Analysis of residential, industrial and commercial sector responses to potential electricity supply constraints in the 1990s

    Energy Technology Data Exchange (ETDEWEB)

    Fisher, Z.J.; Fang, J.M.; Lyke, A.J.; Krudener, J.R.

    1986-09-01

    There is considerable debate over the ability of electric generation capacity to meet the growing needs of the US economy in the 1990s. This study provides new perspective on that debate and examines the possibility of power outages resulting from electricity supply constraints. Previous studies have focused on electricity supply growth, demand growth, and on the linkages between electricity and economic growth. This study assumes the occurrence of electricity supply shortfalls in the 1990s and examines the steps that homeowners, businesses, manufacturers, and other electricity users might take in response to electricity outages.

  2. Junction Quality of SnO2-Based Perovskite Solar Cells Investigated by Nanometer-Scale Electrical Potential Profiling.

    Science.gov (United States)

    Xiao, Chuanxiao; Wang, Changlei; Ke, Weijun; Gorman, Brian P; Ye, Jichun; Jiang, Chun-Sheng; Yan, Yanfa; Al-Jassim, Mowafak M

    2017-11-08

    Electron-selective layers (ESLs) and hole-selective layers (HSLs) are critical in high-efficiency organic-inorganic lead halide perovskite (PS) solar cells for charge-carrier transport, separation, and collection. We developed a procedure to assess the quality of the ESL/PS junction by measuring potential distribution on the cross section of SnO2-based PS solar cells using Kelvin probe force microscopy. Using the potential profiling, we compared three types of cells made of different ESLs but otherwise having an identical device structure: (1) cells with PS deposited directly on bare fluorine-doped SnO2 (FTO)-coated glass; (2) cells with an intrinsic SnO2 thin layer on the top of FTO as an effective ESL; and (3) cells with the SnO2 ESL and adding a self-assembled monolayer (SAM) of fullerene. The results reveal two major potential drops or electric fields at the ESL/PS and PS/HSL interfaces. The electric-field ratio between the ESL/PS and PS/HSL interfaces increased in devices as follows: FTO SnO2-ESL SnO2 + SAM; this sequence explains the improvements of the fill factor (FF) and open-circuit voltage (Voc). The improvement of the FF from the FTO to SnO2-ESL cells may result from the reduction in voltage loss at the PS/HSL back interface and the improvement of Voc from the prevention of hole recombination at the ESL/PS front interface. The further improvements with adding an SAM is caused by the defect passivation at the ESL/PS interface, and hence, improvement of the junction quality. These nanoelectrical findings suggest possibilities for improving the device performance by further optimizing the SnO2-based ESL material quality and the ESL/PS interface.

  3. The electric potential of particles in interstellar space released from a nuclear waste payload

    Science.gov (United States)

    Williams, A. C.

    1980-01-01

    Mechanisms for charging a grain in the interplanetary medium include: (1) capture of solar wind electrons; (2) capture of solar wind protons; (3) ejection of electrons through the photoelectric effect due to the solar radiation; (4) escape of beta particles from beta emitters in the grain; and (5) escape of alpha particles from alpha emitters in the grain. The potentials on both nonradioactive and radioactive grains are considered with relation to particle size and time, and the distance from the Sun. Numerical results are presented where the waste mix is assumed to be PW-4b.

  4. Scenario analysis on CO{sub 2} emissions reduction potential in China's electricity sector

    Energy Technology Data Exchange (ETDEWEB)

    Cai Wenjia [Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084 (China); Wang Can [Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084 (China)], E-mail: canwang@tsinghua.edu.cn; Wang Ke; Zhang Ying; Chen Jining [Department of Environmental Science and Engineering, Tsinghua University, Beijing 100084 (China)

    2007-12-15

    With the approach of the year 2012, a new round of international negotiations has energized the entire climate change community. With this, analyses on sector-based emissions reduction and mitigation options will provide the necessary information to form the debate. In order to assess the CO{sub 2} emissions reduction potential of China's electricity sector, this research employs three scenarios based on the 'long-range energy alternative planning system' (LEAP) model to simulate the different development paths in this sector. The baseline scenario, the current policy scenario, and the new policy scenario seek to gradually increase the extent of industrial restructuring and technical advancement. Results imply that energy consumption and CO{sub 2} emission in China's electricity sector will rise rapidly in all scenarios until 2030-triple or quadruple the 2000 level; however, through structural adjustment in China's electricity sector, and through implementing technical mitigation measures, various degrees of abatement can be achieved. These reductions range from 85 to 350 million tons CO{sub 2} per year-figures that correspond to different degrees of cost and investment. Demand side management and circulating fluidized bed combustion (CFBC) (ranked in order) are employed prior to use to realize emissions reduction, followed by supercritical plants and the renovation of conventional thermal power plants. In the long term, nuclear and hydropower will play the dominant role in contributing to emissions reduction. It is also suggested that a 'self-restraint' reduction commitment should be employed to help contribute to the reduction of emission intensity, an avenue that is more practical for China in light of its current development phase. Setting the year 2000 as the base year, the intensity reduction target could possibly range from 4.2% to 19.4%, dependent on the implementation effectiveness of various mitigation options.

  5. Continuous and embedded solutions for SHM of concrete structures using changing electrical potential in self-sensing cement-based composites

    Science.gov (United States)

    Downey, Austin; Garcia-Macias, Enrique; D'Alessandro, Antonella; Laflamme, Simon; Castro-Triguero, Rafael; Ubertini, Filippo

    2017-04-01

    Interest in the concept of self-sensing structural materials has grown in recent years due to its potential to enable continuous low-cost monitoring of next-generation smart-structures. The development of cement-based smart sensors appears particularly well suited for monitoring applications due to their numerous possible field applications, their ease of use and long-term stability. Additionally, cement-based sensors offer a unique opportunity for structural health monitoring of civil structures because of their compatibility with new or existing infrastructure. Particularly, the addition of conductive carbon nanofillers into a cementitious matrix provides a self-sensing structural material with piezoresistive characteristics sensitive to deformations. The strain-sensing ability is achieved by correlating the external loads with the variation of specific electrical parameters, such as the electrical resistance or impedance. Selection of the correct electrical parameter for measurement to correlate with features of interest is required for the condition assessment task. In this paper, we investigate the potential of using altering electrical potential in cement-based materials doped with carbon nanotubes to measure strain and detect damage in concrete structures. Experimental validation is conducted on small-scale specimens including a steel-reinforced beam of conductive cement paste. Comparisons are made with constant electrical potential and current methods commonly found in the literature. Experimental results demonstrate the ability of the changing electrical potential at detecting features important for assessing the condition of a structure.

  6. GAMMA RADIATION INTERACTS WITH MELANIN TO ALTER ITS OXIDATION-REDUCTION POTENTIAL AND RESULTS IN ELECTRIC CURRENT PRODUCTION

    Energy Technology Data Exchange (ETDEWEB)

    Turick, C.; Ekechukwu, A.; Milliken, C.

    2011-05-17

    The presence of melanin pigments in organisms is implicated in radioprotection and in some cases, enhanced growth in the presence of high levels of ionizing radiation. An understanding of this phenomenon will be useful in the design of radioprotective materials. However, the protective mechanism of microbial melanin in ionizing radiation fields has not yet been elucidated. Here we demonstrate through the electrochemical techniques of chronoamperometry, chronopotentiometry and cyclic voltammetry that microbial melanin is continuously oxidized in the presence of gamma radiation. Our findings establish that ionizing radiation interacts with melanin to alter its oxidation-reduction potential. Sustained oxidation resulted in electric current production and was most pronounced in the presence of a reductant, which extended the redox cycling capacity of melanin. This work is the first to establish that gamma radiation alters the oxidation-reduction behavior of melanin, resulting in electric current production. The significance of the work is that it provides the first step in understanding the initial interactions between melanin and ionizing radiation taking place and offers some insight for production of biomimetic radioprotective materials.

  7. Electric and Hybrid Vehicle System Research and Development Project: Hybrid Vehicle Potential Assessment. Volume IV. Series systems

    Energy Technology Data Exchange (ETDEWEB)

    Popinski, Z.

    1979-09-30

    In the Hybrid Vehicle Potential Assessment Task three major powertrain configurations (parallel, parallel with flywheel, and series) were studied. An evaluation of the series configuration is presented. The series configuration has the advantage that the engine is mechanically uncoupled from the wheels and can be operated at its best economy point much of the time. The mechanical energy produced by the engine is converted through a generator into electrical energy which is used to drive the motor or charge the batteries. This configuration offers a good degree of flexibility. It has the disadvantage that substantial losses of energy occur since the mechanical energy from the engine passes through several components before being used to drive the wheels. The energy produced by the engine is reduced by the product of efficiencies of components connected in series. Trade-offs involved in the study of the series configuration were directed toward establishing the size of the engine, motor and generator to meet vehicle acceleration performance; determining what level to operate the engine, and determining when to use the battery. These results were then used in the electric range simulation.

  8. Wind-generated Electricity in China: Decreasing Potential, Inter-annual Variability and Association with Changing Climate.

    Science.gov (United States)

    Sherman, Peter; Chen, Xinyu; McElroy, Michael B

    2017-11-24

    China hosts the world's largest market for wind-generated electricity. The financial return and carbon reduction benefits from wind power are sensitive to changing wind resources. Wind data derived from an assimilated meteorological database are used here to estimate what the wind generated electricity in China would have been on an hourly basis over the period 1979 to 2015 at a geographical resolution of approximately 50 km × 50 km. The analysis indicates a secular decrease in generating potential over this interval, with the largest declines observed for western Inner Mongolia (15 ± 7%) and the northern part of Gansu (17 ± 8%), two leading wind investment areas. The decrease is associated with long-term warming in the vicinity of the Siberian High (SH), correlated also with the observed secular increase in global average surface temperatures. The long-term trend is modulated by variability relating to the Pacific Decadal Oscillation (PDO) and the Arctic Oscillation (AO). A linear regression model incorporating indices for the PDO and AO, as well as the declining trend, can account for the interannual variability of wind power, suggesting that advances in long-term forecasting could be exploited to markedly improve management of future energy systems.

  9. Electrical potential-assisted DNA hybridization. How to mitigate electrostatics for surface DNA hybridization.

    Science.gov (United States)

    Tymoczko, Jakub; Schuhmann, Wolfgang; Gebala, Magdalena

    2014-12-24

    Surface-confined DNA hybridization reactions are sensitive to the number and identity of DNA capture probes and experimental conditions such as the nature and the ionic strength of the electrolyte solution. When the surface probe density is high or the concentration of bulk ions is much lower than the concentration of ions within the DNA layer, hybridization is significantly slowed down or does not proceed at all. However, high-density DNA monolayers are attractive for designing high-sensitivity DNA sensors. Thus, circumventing sluggish DNA hybridization on such interfaces allows a high surface concentration of target DNA and improved signal/noise ratio. We present potential-assisted hybridization as a strategy in which an external voltage is applied to the ssDNA-modified interface during the hybridization process. Results show that a significant enhancement of hybridization can be achieved using this approach.

  10. Potential of exchange of electric power between the electric systems of Brazil and Argentina; Potencial de intercambio de energia eletrica entre os sistemas eletricos do Brasil e da Argentina

    Energy Technology Data Exchange (ETDEWEB)

    Zanette, Andre Luiz [Petroleo Brasileiro S.A. (PETROBRAS), Rio de Janeiro, RJ (Brazil)

    2010-07-01

    Integration of power generation and transmission systems can provide a better utilization of energy resources and increase systems reliability. Given that Brazilian interconnection with Argentina, which represents the major Brazilian interconnection with neighboring countries, is used basically in winter months or in situations of unfavorable hydrology, this paper aims to demonstrate that power exchange between these two countries can be much higher than currently observed, resulting in greater benefits for producers and consumers of electricity in both countries, and that these benefits can also be obtained through better use of existing and planned interconnections between South American countries. To estimate the potential for power exchange between the electrical systems of Brazil and Argentina, three sets of simulations using the Brazilian Energy Plan (PDE 2010-2019) deck for Newave model, available from EPE, were made. These simulations consider the possibility that: (i) electricity is exported from Brazil to Argentina (II) electricity is imported from Argentina and (III) electricity is exchanged in both directions. The simulation results indicate that, despite rising operational marginal costs, significant amounts of electricity can be exported to Argentina at relatively low prices, depending on hydrological conditions. Moreover, the possibility of electricity imports from Argentina can reduce the operational costs of Brazilian system, as well as minimizing the risk of shortages. This work shows that the interconnections between Brazil and Argentina may be better used to benefit producers and consumers in both countries. Therefore, it is necessary to define an effective contractual and operational model for power trading and electricity transfer between these countries with the objective of promoting stability and mutual trust. Finally, studies to evaluate the potential for electricity exchange between South American countries are specially important

  11. Testing of the permanent magnet material Mn-Al-C for potential use in propulsion motors for electric vehicles

    Science.gov (United States)

    Abdelnour, Z.; Mildrun, H.; Strant, K.

    1981-01-01

    The development of Mn-Al-C permanent magnets is reviewed. The general properties of the material are discussed and put into perspective relative to alnicos and ferrites. The traction motor designer's demands of a permanent magnet for potential use in electric vehicle drives are reviewed. Tests determined magnetic design data and mechanical strength properties. Easy axis hysteresis and demagnetization curves, recoil loops and other minor loop fields were measured over a temperature range from -50 to 150 C. Hysteresis loops were also measured for three orthogonal directions (the one easy and two hard axes of magnetization). Extruded rods of three different diameters were tested. The nonuniformity of properties over the cross section of the 31 mm diameter rod was studied. Mechanical compressive and bending strength at room temperature was determined on individual samples from the 31 mm rod.

  12. Cell surface hydrophobicity and colony morphology of Trichosporon asahii clinical isolates.

    Science.gov (United States)

    Ichikawa, Tomoe; Hirata, Chihiro; Takei, Mizuki; Tagami, Naoyuki; Murasawa, Hiromi; Ikeda, Reiko

    2017-03-01

    Trichosporon asahii is a pathogenic basidiomycetous yeast. Individual strains of T. asahii have different colony morphologies. However, it is not clear whether cell surface phenotypes differ among the colony morphologies. Here we characterized the cell surface hydrophobicity and analysed the carbohydrate contents of the cell surface polysaccharides in T. asahii clinical isolates with various colony morphologies. Among the three distinctive colony morphologies obtained from one clinical isolate, the white-type morphology exhibited higher hydrophobicity. The hydrophobicity of heat-killed T. asahii cells was greatly reduced after periodate oxidation of the cell surface carbohydrates. Furthermore, the cell wall and extracellular polysaccharide components differed among the morphologies. Our results suggest that T. asahii cell surface hydrophobicity is affected by cell surface carbohydrate composition. Copyright © 2016 John Wiley & Sons, Ltd. Copyright © 2016 John Wiley & Sons, Ltd.

  13. Cell-surface display of enzymes by the yeast Saccharomyces cerevisiae for synthetic biology.

    Science.gov (United States)

    Tanaka, Tsutomu; Kondo, Akihiko

    2015-02-01

    In yeast cell-surface displays, functional proteins, such as cellulases, are genetically fused to an anchor protein and expressed on the cell surface. Saccharomyces cerevisiae, which is often utilized as a cell factory for the production of fuels, chemicals, and proteins, is the most commonly used yeast for cell-surface display. To construct yeast cells with a desired function, such as the ability to utilize cellulose as a substrate for bioethanol production, cell-surface display techniques for the efficient expression of enzymes on the cell membrane need to be combined with metabolic engineering approaches for manipulating target pathways within cells. In this Minireview, we summarize the recent progress of biorefinery fields in the development and application of yeast cell-surface displays from a synthetic biology perspective and discuss approaches for further enhancing cell-surface display efficiency. © FEMS 2015. All rights reserved. For permissions, please e-mail: journals.permission@oup.com.

  14. Neuronal excitation and permeabilization by 200-ns pulsed electric field: An optical membrane potential study with FluoVolt dye.

    Science.gov (United States)

    Pakhomov, Andrei G; Semenov, Iurii; Casciola, Maura; Xiao, Shu

    2017-07-01

    Electric field pulses of nano- and picosecond duration are a novel modality for neurostimulation, activation of Ca 2+ signaling, and tissue ablation. However it is not known how such brief pulses activate voltage-gated ion channels. We studied excitation and electroporation of hippocampal neurons by 200-ns pulsed electric field (nsPEF), by means of time-lapse imaging of the optical membrane potential (OMP) with FluoVolt dye. Electroporation abruptly shifted OMP to a more depolarized level, which was reached within 10s), so cells remained above the resting OMP level for at least 20-30s. Activation of voltage-gated sodium channels (VGSC) enhanced the depolarizing effect of electroporation, resulting in an additional tetrodotoxin-sensitive OMP peak in 4-5ms after nsPEF. Omitting Ca 2+ in the extracellular solution did not reduce the depolarization, suggesting no contribution of voltage-gated calcium channels (VGCC). In 40% of neurons, nsPEF triggered a single action potential (AP), with the median threshold of 3kV/cm (range: 1.9-4kV/cm); no APs could be evoked by stimuli below the electroporation threshold (1.5-1.9kV/cm). VGSC opening could already be detected in 0.5ms after nsPEF, which is too fast to be mediated by the depolarizing effect of electroporation. The overlap of electroporation and AP thresholds does not necessarily reflect the causal relation, but suggests a low potency of nsPEF, as compared to conventional electrostimulation, for VGSC activation and AP induction. Copyright © 2017 Elsevier B.V. All rights reserved.

  15. Vertical electrical sounding to delineate the potential aquifer zones for drinking water in Niamey city, Niger, Africa

    Science.gov (United States)

    Choudhury, Joy; Kumar, K. Lohith; Nagaiah, E.; Sonkamble, S.; Ahmed, Shakeel; Kumar, Venay

    2017-08-01

    Niger is a landlocked African country and the only source of surface water is the Niger River which flows in the western part of Niger and only few villages near to the river gets benefited from it, leaving most of the areas dependent on groundwater solely. The groundwater resources in Niger are mainly used for drinking, livestock and domestic needs. It can be observed that the water exploitation is minimal there due to several factors like undeveloped areas, less population, limited wells, rain-fed irrigation, etc. The delineation of potential aquifer zones is an important aspect for groundwater prospecting. Hence, the direct current (DC) resistivity soundings method also known as vertical electrical sounding (VES) is one of the most applied geophysical techniques for groundwater prospecting that was used in the capital city, Niamey of Niger. Twelve VES surveys, each of AB spacing 400 m were carried out in lateritic and granitic rock formations with a view to study the layer response and to delineate the potential zones. Potential aquifer zones were at shallow depth ranging from 10 to 25 m for the drilled borehole depth of 80-85 m in every village. Analysis of the result showed a good correlation between the acquired data and the lithologs.

  16. [Synchronization and propagation of electrical potentials in neural networks of the brain cortex].

    Science.gov (United States)

    Marchenko, V G; Zaĭchenko, M I

    2014-01-01

    On the basis of the published data the scheme is proposed which explains the spread and synhronization of oscillatory activity in cortex. The main property of the neocortex is the existence of vertically oriented functional columns. Within and between the neuronal columns exist a feedforward and feedback morphological and functional connections. At the certain conditions inside the single module temporal windows are created using inhibitory process, and a synchronized activity can be generated of variable frequency (oscillations). The activity in the columns is enhanced by the synchronous involvement of great amount of neurons which is expressed in the form of local field potentials of high amplitude. Further the information about arisen in a single generator activity is transmitted through direct links to the nearby generator or to the group of such generators. In the nearby generator the activity increases and is transferred to the next generator. At the same time the signal is transmitted through feedback to the primary generator, and the activity is terminated until the next cycle will be initiated and so on along the cortex. The most important notion is that in the transfer of activity from one generator to another is involved a small number of elements. The major part of recorded oscillations of different frequency or epileptiform discharges, is not transmitted via the brain, but is generated in each module according to the characteristics transferred to it. The generation of epileptiform spikes occurs in cases when balance of inhibition determined by GABA A and GABA B receptors is disturbed.

  17. Assessment of wind energy potential and optimal electricity generation in Borj-Cedria, Tunisia

    Energy Technology Data Exchange (ETDEWEB)

    Dahmouni, A.W.; Kerkeni, C. [Laboratoire de Maitrise de l' Energie Eolienne et de Valorisation Energetique des Dechets, Centre de Recherche et des Technologies de l' Energie, Technopole de Borj-Cedria, BP 95, Hammam Lif 2050 (Tunisia); Ben Salah, M. [Laboratoire des Procedees Thermiques, Centre de Recherche et technologies de l' Energie, Centre de Recherche et des Technologies de l' Energie, Technopole de Borj-Cedria, BP 95 Hammam Lif 2050 (Tunisia); Askri, F.; Ben Nasrallah, S. [Laboratoire d' Etudes des Systemes Thermiques et Energetiques, Ecole Nationale d' Ingenieurs de Monastir, avenue Ibn El Jazzar 5019, Monastir (Tunisia)

    2011-01-15

    In the last century, several climate changes have been observed in regions all over the world. The main cause of these climatic changes is the rise of fossil fuel uses, which is due to the important demographic and industrial development. These negative effects have forced scientists to draw attention to renewable energy sources, which are the most suitable solution in the future. In this paper, wind energy potential was estimated using the wind speed data collected by two meteorological stations installed in the Centre of Research and Technologies of Energy (CRTEn) in the Borj-Cedria area. The data collected at 30, 20 and 10 m height during 2008 and 2009, have permitted us to estimate the seasonal mean wind speed, wind speed distribution and wind power density. The results have been used to estimate the net energy output of seven 1.5 MW wind turbines with taken account the air density correction and the power losses in wind farm. This comparative simulation shows difference in wind generators production and allows us to choose the best wind turbine adapted to the site conditions. (author)

  18. National potential of saving of electricity in domestic refrigeration; Potencial nacional de ahorro de electricidad en refrigeracion domestica

    Energy Technology Data Exchange (ETDEWEB)

    Arroyo C, Fernando [Universidad Nacional Autonoma de Mexico (Mexico); Ambriz G, Juan Jose; Romero P, Hernando [Universidad Autonoma Metropolitana, (Mexico)

    2003-07-01

    In the present paper the evaluation of the potential of saving of electrical energy in Mexico is presented, by means of the substitution of old refrigerators of low efficiency by modern high efficiency ones. This potential is very ample, since the replacement of the old models by new follows a very slow curve, if the economic difficulties of the Mexican families are considered and that, on the other hand, the technology of the domestic refrigerators is so reliable that they have a very long life and they are not easily rejected. In field work have been found refrigerators operating with ages of more than 30 years and still 40. In Mexico, it is estimated that the refrigeration is responsible for around 29% of the of electricity consumption in homes settled in tempered climate and of 14 % in those of warm climate in which air conditioning is used. The proposal of this work is the organization of a governmental program of accelerated substitution of refrigerators that helps to acquire an efficient refrigerator and to reject the old ones. The results indicate that if a total renovation of the park of domestic refrigerators is made, the saving of electrical energy would reach the 5.2 TWh/a which represents the 44.5% of the total consumption of 11.7 TWh in a year. [Spanish] En el presente trabajo se presenta la evaluacion del potencial de ahorro de energia electrica en Mexico, mediante la substitucion de refrigeradores antiguos de baja eficiencia por modernos de alta eficiencia. Este potencial es muy amplio, ya que el reemplazo de los modelos viejos por nuevos sigue una curva muy lenta, si se consideran las dificultades economicas de las familias mexicanas y que, por otro lado, la tecnologia de los refrigeradores domesticos es tan confiable que tienen una vida muy larga y no se desechan facilmente. En trabajos de campo se han encontrado refrigeradores operando con edades de mas de 30 anos y aun 40. En Mexico, se estima que la refrigeracion es responsable de alrededor del

  19. Monitoring the Dynamics of Water Flow at a High-Mountain Permafrost Site Using Electrical Self-Potential Measurements

    Science.gov (United States)

    Kemna, A.; Weigand, M.; Wagner, F.; Hilbich, C.; Hauck, C.

    2016-12-01

    Flow of (liquid) water plays a crucial role in the dynamics of coupled thermo-hydro-mechanical processes in terrestrial permafrost systems. To better understand these processes in the active layer of permafrost regions, with the ultimate goal of adequately incorporating them in numerical models for improved scenario prediction, monitoring approaches offering high spatial and temporal resolution, areal coverage, and especially sensitivity to subsurface water flow, are highly desired. This particularly holds for high-mountain slopes, where strong variability in topography, precipitation, and snow cover, along with significant subsurface soil/rock heterogeneity, gives rise to complex spatio-temporal patterns of water flow during seasonal thawing and freezing periods. The electrical self-potential (SP) method is well known to, in theory, meeting the above monitoring demands by measuring the electrical streaming potential which is generated at the microscopic scale when water flows along electrically non-neutral interfaces. Despite its inherent sensitivity to subsurface water flow, the SP method has not yet been used for the monitoring of high-mountain permafrost sites. We here present first results from an SP monitoring survey conducted at the Schilthorn (2970 m asl) in the Bernese Alps, Switzerland, where SP data have been collected since September 2013 at a sampling rate of 10 min on a permanently installed array of 12 non-polarizing electrodes covering an area of 35 m by 15 m. While the SP time series exhibit systematic daily variations, with part of the signal clearly correlated with temperature, in particular in the snow-free periods, the largest temporal changes in the SP signal occur in spring, when the snow cover melts and thawing sets on in the active layer. The period of higher temporal SP variations continues until autumn, when the signal gradually returns to relatively low variations, coinciding with the freezing of the ground. Our results suggest that the

  20. Atomic Force Microscopy in Microbiology: New Structural and Functional Insights into the Microbial Cell Surface

    Science.gov (United States)

    2014-01-01

    ABSTRACT Microbial cells sense and respond to their environment using their surface constituents. Therefore, understanding the assembly and biophysical properties of cell surface molecules is an important research topic. With its ability to observe living microbial cells at nanometer resolution and to manipulate single-cell surface molecules, atomic force microscopy (AFM) has emerged as a powerful tool in microbiology. Here, we survey major breakthroughs made in cell surface microbiology using AFM techniques, emphasizing the most recent structural and functional insights. PMID:25053785

  1. Modulation of Cortical Motor Evoked Potential After Stroke During Electrical Stimulation of the Lateral Cerebellar Nucleus.

    Science.gov (United States)

    Park, Hyun-Joo; Furmaga, Havan; Cooperrider, Jessica; Gale, John T; Baker, Kenneth B; Machado, Andre G

    2015-01-01

    Deep brain stimulation (DBS) targeting the dentato-thalamo-cortical (DTC) pathway at its origin in the lateral cerebellar nucleus (LCN) has been shown to enhance motor recovery in a rodent model of cortical ischemia. LCN DBS also yielded frequency-specific changes in motor cortex excitability in the normal brain, indexed by motor evoked potential (MEP) amplitude. To investigate the effect of cortical stroke on cortical motor excitability in a rodent ischemia model and to measure the effects of LCN DBS on post-ischemia excitability as a function of stimulation parameters. Adult Sprague-Dawley rats were divided into two groups: naïve and stroke, with cortical ischemia induced through multiple, unilateral endothelin-1 injections. All animals were implanted with a bipolar electrode in the LCN opposite the affected hemisphere. MEPs were elicited from the affected hemisphere using intracortical microstimulation (ICMS) techniques. Multiple LCN DBS parameters were examined, including isochronal stimulation at 20, 30, 50, and 100 Hz as well as a novel burst stimulation pattern. ICMS-evoked MEPs were reduced in stroke (n = 10) relative to naïve (n = 12) animals. However, both groups showed frequency-dependent augmentation of cortical excitability in response to LCN DBS. In the naïve group, LCN DBS increased MEPs by 22-58%, while in the stroke group, MEPs were enhanced by 9-41% compared to OFF-DBS conditions. Activation of the DTC pathway increases cortical excitability in both naïve and post-stroke animals. These effects may underlie, at least partially, functional reorganization and therapeutic benefits associated with chronic LCN DBS in post-stroke animals. Copyright © 2015 Elsevier Inc. All rights reserved.

  2. Illumination of growth, division and secretion by metabolic labeling of the bacterial cell surface

    Science.gov (United States)

    Siegrist, M. Sloan; Swarts, Benjamin M.; Fox, Douglas M.; Lim, Shion An; Bertozzi, Carolyn R.

    2015-01-01

    The cell surface is the essential interface between a bacterium and its surroundings. Composed primarily of molecules that are not directly genetically encoded, this highly dynamic structure accommodates the basic cellular processes of growth and division as well as the transport of molecules between the cytoplasm and the extracellular milieu. In this review, we describe aspects of bacterial growth, division and secretion that have recently been uncovered by metabolic labeling of the cell envelope. Metabolite derivatives can be used to label a variety of macromolecules, from proteins to non-genetically-encoded glycans and lipids. The embedded metabolite enables precise tracking in time and space, and the versatility of newer chemoselective detection methods offers the ability to execute multiple experiments concurrently. In addition to reviewing the discoveries enabled by metabolic labeling of the bacterial cell envelope, we also discuss the potential of these techniques for translational applications. Finally, we offer some guidelines for implementing this emerging technology. PMID:25725012

  3. Chemical and Enzymatic Strategies for Bacterial and Mammalian Cell Surface Engineering.

    Science.gov (United States)

    Bi, Xiaobao; Yin, Juan; Chen Guanbang, Ashley; Liu, Chuan-Fa

    2018-01-17

    Cell surface serves important functions such as the regulation of cell-cell and cell-environment interactions. The understanding and manipulation of the cell surface is important for a wide range of fundamental studies of cellular behavior and for biotechnological and medical applications. With the rapid advance of biology, chemistry and materials science, many strategies have been developed for the functionalization of bacterial and mammalian cell surfaces. Here, we review the recent development of chemical and enzymatic approaches to cell surface engineering with particular emphasis on discussing the advantages and limitations of each of these strategies. © 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. Sorption of heavy metals by prepared bacterial cell surfaces

    Energy Technology Data Exchange (ETDEWEB)

    Churchill, S.A.; Walters, J.V.; Churchill, P.F. [Univ. of Alabama, Tuscaloosa, AL (United States)

    1995-10-01

    Prepared biomass from two Gram-negative and one Gram-positive bacterial strains was examined for single, binary, and quaternary mixtures of polyvalent metal cation binding to cell surfaces. The biosorption of {sub 24}Cr{sup 3+}, {sub 27}Co{sup 2+}, {sub 28}Ni{sup 2+}, and {sub 29}Cu{sup 2+} for each bacterial cell type was evaluated using a batch equilibrium method. The binding of each metal by all three bacterial cells could be described by the Freundlich sorption model. The isotherm binding constants suggest that E. coli cells are the most efficient at binding copper, chromium, and nickel; and M. luteus adsorbs cobalt most efficiently. The K-values for copper bound to P. aeruginosa and E. coli are > 2-fold and > 8-fold greater, respectively, than previous reported for intact cells. The general metal-affinity series observed was Cr{sup 3+} > Cu{sup 2+} > Ni{sup 2+} > Co{sup 2+}. There was a marked lower affinity of all biosorbents for Co{sup 2+} and Ni{sup 2+}. M. luteus and E. coli had a strong preference for Co{sup 2+} over Ni{sup 2+}. Metal-binding enhancement could be ascribed to increased cell barrier surface porosity to metal-bearing solutions.

  5. Antifouling property of highly oleophobic substrates for solar cell surfaces

    Science.gov (United States)

    Fukada, Kenta; Nishizawa, Shingo; Shiratori, Seimei

    2014-03-01

    Reduction of solar cell conversion efficiency by bird spoor or oil smoke is a common issue. Maintaining the surface of solar cells clean to retain the incident light is of utmost importance. In this respect, there has been growing interest in the area of superhydrophobicity for developing water repelling and self-cleaning surfaces. This effect is inspired by lotus leaves that have micro papillae covered with hydrophobic wax nanostructures. Superhydrophobic surfaces on transparent substrates have been developed for removing contaminants from solar cell surfaces. However, oil cannot be removed by superhydrophobic effect. In contrast, to prevent bird spoor, a highly oleophobic surface is required. In a previous study, we reported transparent-type fabrics comprising nanoparticles with a nano/micro hierarchical structure that ensured both oleophobicity and transparency. In the current study, we developed new highly oleophobic stripes that were constructed into semi-transparent oleophobic surfaces for solar cells. Solar cell performance was successfully maintained; the total transmittance was a key factor for determining conversion efficiency.

  6. Biochemical engineering of cell surface sialic acids stimulates axonal growth.

    Science.gov (United States)

    Büttner, Bettina; Kannicht, Christoph; Schmidt, Carolin; Löster, Klemens; Reutter, Werner; Lee, Hye-Youn; Nöhring, Sabine; Horstkorte, Rüdiger

    2002-10-15

    Sialylation is essential for development and regeneration in mammals. Using N-propanoylmannosamine, a novel precursor of sialic acid, we were able to incorporate unnatural sialic acids with a prolonged N-acyl side chain (e.g., N-propanoylneuraminic acid) into cell surface glycoconjugates. Here we report that this biochemical engineering of sialic acid leads to a stimulation of neuronal cells. Both PC12 cells and cerebellar neurons showed a significant increase in neurite outgrowth after treatment with this novel sialic acid precursor. Furthermore, also the reestablishment of the perforant pathway was stimulated in brain slices. In addition, we surprisingly identified several cytosolic proteins with regulatory functions, which are differentially expressed after treatment with N-propanoylmannosamine. Because sialic acid is the only monosaccharide that is activated in the nucleus, we hypothesize that transcription could be modulated by the unnatural CMP-N-propanoylneuraminic acid and that sialic acid activation might be a general tool to regulate cellular functions, such as neurite outgrowth.

  7. Cell surface lectin array: parameters affecting cell glycan signature.

    Science.gov (United States)

    Landemarre, Ludovic; Cancellieri, Perrine; Duverger, Eric

    2013-04-01

    Among the "omics", glycomics is one of the most complex fields and needs complementary strategies of analysis to decipher the "glycan dictionary". As an alternative method, which has developed since the beginning of the 21st century, lectin array technology could generate relevant information related to glycan motifs, accessibility and a number of other valuable insights from molecules (purified and non-purified) or cells. Based on a cell line model, this study deals with the key parameters that influence the whole cell surface glycan interaction with lectin arrays and the consequences on the interpretation and reliability of the results. The comparison between the adherent and suspension forms of Chinese Hamster Ovary (CHO) cells, showed respective glycan signatures, which could be inhibited specifically by neoglycoproteins. The modifications of the respective glycan signatures were also revealed according to the detachment modes and cell growth conditions. Finally the power of lectin array technology was highlighted by the possibility of selecting and characterizing a specific clone from the mother cell line, based on the slight difference determination in the respective glycan signatures.

  8. EXAFS Study of Uranyl Complexation at Pseudomonas fluorescens Cell Surfaces

    Science.gov (United States)

    Bencheikh, R.; Bargar, J. R.; Tebo, B. M.

    2002-12-01

    Little is known about the roles of microbial biomass as a sink and source for uranium in contaminated aquifers, nor of the impact of bacterial biochemistry on uranium speciation in the subsurface. A significant role is implied by the high affinities of both Gram positive and Gram negative cells for binding uranyl (UO2{ 2+}). In the present study, Extended X-ray Absorption Fine Structure (EXAFS) spectroscopy was used to identify membrane functional groups involved in uranyl binding to the Gram negative bacterium Pseudomonas fluorescens from pH 3 to pH 8. Throughout this pH-range, EXAFS spectra can be described primarily in terms of coordination of carboxylic groups to uranyl. U-C distances characteristic of 4-, 5- and 8- membered rings were observed, as well as the possibility of phosphato groups. Both shell-by-shell fits and principle component analyses indicate that the functional groups involved in binding of uranyl to the cell surface do not vary systematically across the pH range investigated. This result contrasts with EXAFS results of uranyl sorbed to Gram positive bacteria, and suggests an important role for long-chain carboxylate-terminated membrane functional groups in binding uranyl.

  9. Redistribution of a major cell surface glycoprotein during cell movement.

    Science.gov (United States)

    Jacobson, K; O'Dell, D; Holifield, B; Murphy, T L; August, J T

    1984-11-01

    The distribution in living cells of an 80,000-dalton major cell surface glycoprotein of murine fibroblasts has been studied by use of monoclonal antibodies. The presence of the molecule throughout the plasma membrane and on the substrate attached surface of the cell was demonstrated by immunofluorescence. Cell growth kinetics were not altered and the cells remained motile in the presence of the antibody. The uniform distribution of the direct immunofluorescence stain persisted for long periods (greater than 100 h), which indicates that the fluorescent monoclonal antibodies may be used to trace antigen surface distribution during cell functions. In motile cells, but not G0 or confluent cells, the degree of fluorescent staining decreased toward the leading edge; this gradient increased markedly during the time that the antibody was bound to the cells. However, the gradation was not seen with the lipid probe, dihexadecylindocarbocyanine. The antigen was "patched" only by the application of a second antibody directed to the rat monoclonal antibody and the relationships of these patches to the underlying cytoskeleton were characterized.

  10. Generation of arming yeasts with active proteins and peptides via cell surface display system: cell surface engineering, bio-arming technology.

    Science.gov (United States)

    Kuroda, Kouichi; Ueda, Mitsuyoshi

    2014-01-01

    The cell surface display system in yeast enables the innovative strategy for improving cellular functions in a wide range of applications such as biofuel production, bioremediation, synthesis of valuable chemicals, recovery of rare metal ions, development of biosensors, and high-throughput screening of proteins/peptides library. Display of enzymes for polysaccharide degradation enables the construction of metabolically engineered whole-cell biocatalyst owing to the accessibility of the displayed enzymes to high-molecular-weight polysaccharides. In addition, along with fluorescence-based activity evaluation, fluorescence-activated cell sorting (FACS), and yeast cell chip, the cell surface display system is an effective molecular tool for high-throughput screening of mutated proteins/peptides library. In this article, we describe the methods for cell surface display of proteins/peptides of interest on yeast, evaluation of display efficiency, and harvesting of the displayed proteins/peptides from cell surface.

  11. Energy storage for the electricity grid : benefits and market potential assessment guide : a study for the DOE Energy Storage Systems Program.

    Energy Technology Data Exchange (ETDEWEB)

    Eyer, James M. (Distributed Utility Associates, Inc., Livermore, CA); Corey, Garth P. (KTech Corporation, Albuquerque, NM)

    2010-02-01

    This guide describes a high-level, technology-neutral framework for assessing potential benefits from and economic market potential for energy storage used for electric-utility-related applications. The overarching theme addressed is the concept of combining applications/benefits into attractive value propositions that include use of energy storage, possibly including distributed and/or modular systems. Other topics addressed include: high-level estimates of application-specific lifecycle benefit (10 years) in $/kW and maximum market potential (10 years) in MW. Combined, these criteria indicate the economic potential (in $Millions) for a given energy storage application/benefit. The benefits and value propositions characterized provide an important indication of storage system cost targets for system and subsystem developers, vendors, and prospective users. Maximum market potential estimates provide developers, vendors, and energy policymakers with an indication of the upper bound of the potential demand for storage. The combination of the value of an individual benefit (in $/kW) and the corresponding maximum market potential estimate (in MW) indicates the possible impact that storage could have on the U.S. economy. The intended audience for this document includes persons or organizations needing a framework for making first-cut or high-level estimates of benefits for a specific storage project and/or those seeking a high-level estimate of viable price points and/or maximum market potential for their products. Thus, the intended audience includes: electric utility planners, electricity end users, non-utility electric energy and electric services providers, electric utility regulators and policymakers, intermittent renewables advocates and developers, Smart Grid advocates and developers, storage technology and project developers, and energy storage advocates.

  12. Quick and effective hyperpolarization of the membrane potential in intact smooth muscle cells of blood vessels by synchronization modulation electric field.

    Science.gov (United States)

    Zhang, Liping; Fang, Zhihui; Chen, Wei

    2012-06-01

    Blood vessel dilation starts from activation of the Na/K pumps and inward rectifier K channels in the vessel smooth muscle cells, which hyperpolarizes the cell membrane potential and closes the Ca channels. As a result, the intracellular Ca concentration reduces, and the smooth muscle cells relax and the blood vessel dilates. Activation of the Na/K pumps and the membrane potential hyperpolarization plays a critical role in blood vessel functions. Previously, we developed a new technique, synchronization modulation, to control the pump functions by electrically entraining the pump molecules. We have applied the synchronization modulation electric field noninvasively to various intact cells and demonstrated the field-induced membrane potential hyperpolarization. We further applied the electric field to blood vessels and investigated the field induced functional changes of the vessels. In this paper, we report the results in a study of the membrane potential change in the smooth muscle cells of mesenteric blood vessels in response to the oscillating electric field. We found that the synchronization modulation electric field can effectively hyperpolarize the muscle membrane potential quickly in seconds under physiological conditions.

  13. Potential Use of Communications and Project Management Systems in Remote Construction Projects: The Case of Saudi Electric Company

    Directory of Open Access Journals (Sweden)

    Bhzad Sidawi

    2012-01-01

    Full Text Available Remote projects have their unique problems that are caused mainly by the remoteness of the project itself thus the loose control over management. This is due to a number of reasons such as lack of human resources and infrastructure. Research studies that were undertaken worldwide - regarding this issue- has highlighted few unique management problems. The aim of this paper is to investigate how far the IT tools would help in managing remote construction projects in the Kingdom of Saudi Arabia (KSA. Due to the lack of previous research regarding remote projects within the Gulf region and the KSA, a pilot study was conducted in 2009 to define and test the wording of questions that are part of the main survey. The main survey was undertaken on Saudi Electric Company (SEC. The study found that IT systems and tools have the potential but this potential is hindered by a number of technical, managerial, staff factors. Therefore, successful application of IT systems would require changes to the present management settings at various levels. This would ensure that the SEC is capable of achieving the full benefit from using these advanced systems.

  14. Potential cost savings from internal/external CO{sub 2} emissions trading in the Korean electric power industry

    Energy Technology Data Exchange (ETDEWEB)

    Lee, Myunghun, E-mail: leemh@inha.ac.kr [Department of International Trade, Inha University, 253 Yonghyun-dong, Nam-gu, Incheon 402-751 (Korea, Republic of)

    2011-10-15

    Korea plans to introduce an emissions trading scheme for the controlling greenhouse gas emissions in 2015. Using Shephard's (1970) output distance function, we first estimate the shadow price of CO{sub 2} for power generators in the Korean fossil-fueled electric generation industry. Then, by assuming that each power generator is required to reduce CO{sub 2} emissions by one ton, we compute the potential cost savings from internal trading among generators within the same plant and from external trading across plants at prevailing market prices. The results indicate that, on average, the generators paid $14.63 to abate one ton of CO{sub 2} emissions in 2007. Plants realized additional gains through external trading. In particular, cost savings from trades between different fuel-fired plants were substantial. - Highlights: > We estimated the shadow price of CO{sub 2} for the Korean steam power plants. > On average, the generators paid $14.63 to abate one ton of CO{sub 2} emissions in 2007. > We computed the potential cost savings from internal trading and external trading. > Plants realized additional gains through external trading.

  15. An Anisotropic Coarse-Grained Model for Proteins Based On Gay-Berne and Electric Multipole Potentials.

    Science.gov (United States)

    Shen, Hujun; Li, Yan; Ren, Pengyu; Zhang, Dinglin; Li, Guohui

    2014-02-10

    Gay-Berne anisotropic potential has been widely used to evaluate the non-bonded interactions between coarse-grained particles being described as elliptical rigid bodies. In this paper, we are presenting a coarse-grained model for twenty kinds of amino acids and proteins, based on the anisotropic Gay-Berne and point electric multipole (EMP) potentials. We demonstrate that the anisotropic coarse-grained model, namely GBEMP model, is able to reproduce many key features observed from experimental protein structures (Dunbrack Library) as well as from atomistic force field simulations (using AMOEBA, AMBER and CHARMM force fields) while saving the computational cost by a factor of about 10~200 depending on specific cases and atomistic models. More importantly, unlike other coarse-grained approaches, our framework is based on the fundamental intermolecular forces with explicit treatment of electrostatic and repulsion-dispersion forces. As a result, the coarse-grained protein model presented an accurate description of non-bonded interactions (particularly electrostatic component) between hetero-/homo-dimers (such as peptide-peptide, peptide-water). In addition, the encouraging performance of the model was reflected by the excellent correlation between GBEMP and AMOEBA models in the calculations of the dipole moment of peptides. In brief, the GBEMP model given here is general and transferable, suitable for simulating complex biomolecular systems.

  16. Potential of electric discharge plasma methods in abatement of volatile organic compounds originating from the food industry.

    Science.gov (United States)

    Preis, S; Klauson, D; Gregor, A

    2013-01-15

    Increased volatile organic compounds emissions and commensurate tightening of applicable legislation mean that the development and application of effective, cost-efficient abatement methods are areas of growing concern. This paper reviews the last two decades' publications on organic vapour emissions from food processing, their sources, impacts and treatment methods. An overview of the latest developments in conventional air treatment methods is presented, followed by the main focus of the paper, non-thermal plasma technology. The results of the review suggest that non-thermal plasma technology, in its pulsed corona discharge configuration, is an emerging treatment method with potential for low-cost, effective abatement of a wide spectrum of organic air pollutants. It is found that the combination of plasma treatment with catalysis is a development trend that demonstrates considerable potential. The as yet relatively small number of plasma treatment applications is considered to be due to the novelty of pulsed electric discharge techniques and a lack of reliable pulse generators and reactors. Other issues acting as barriers to widespread adoption of the technique include the possible formation of stable oxidation by-products, residual ozone and nitrogen oxides, and sensitivity towards air humidity. Copyright © 2012 Elsevier Ltd. All rights reserved.

  17. Thermal conductivity and electrical properties of hybrid SiO2-graphene naphthenic mineral oil nanofluid as potential transformer oil

    Science.gov (United States)

    Qing, Soo Hui; Rashmi, W.; Khalid, M.; Gupta, T. C. S. M.; Nabipoor, M.; Taghi Hajibeigy, Mohammad

    2017-01-01

    Hybrid SiO2-graphene nanoparticles were synthesised by sol gel centrifugation technique under four different pH levels ranging from 9 to 12. Stability, thermal conductivity, viscosity and electrical conductivity of hybrid SiO2-graphene and pure graphene dispersed in naphthenic oil were investigated. Nanofluids were synthesied at three different nanoparticle concentrations (0.01, 0.04 and 0.08 wt%) while the temperature was varied from 20 °C to 100 °C. Field emission scanning electron microscopy (FESEM), x-ray spectroscopy and Fourier transform infrared (FTIR) spectrometer show successful coating of SiO2 on graphene surface. The growth units and size distribution of SiO2 nanoparticles increased with pH level. Moreover, the presence of SiO2 improved the dispersion behaviour of the nanofluid as confirmed by visual observation and UV-Vis studies. Zeta potential measurements show the hybrid nanofluids at pH 11 are most stable due to its optimum amount and size of SiO2 coated on graphene surface while at pH 12 shows least stability due to precipitation. The presence of SiO2 on graphene further enhanced the thermal conductivity by 80% at pH 9. Also, the viscosity of hybrid nanofluids was higher than pure graphene based nanofluids due to increase in density and particle size. Moreover, the addition of hybrid SiO2-graphene nanoparticles significantly reduced the electrical conductivity enhancement of base fluid from 557% to 97%.

  18. Estimate of Low/Mid-Latitude Conductances, Electric Potential and Neutral Winds using IDA4D and EMPIRE

    Science.gov (United States)

    Bust, G. S.; Makela, J. J.; Harding, B. J.; Heelis, R. A.; Bishop, R. L.

    2015-12-01

    In this talk we will focus on the current ability of the ionosphere-thermosphere data assimilative models, Ionospheric Data Assimilation Four Dimensional (IDA4D) and Estimating Model Parameters from Ionospheric Reverse Engineering (EMPIRE), to estimate low and mid latitude ionosphere-thermosphere (IT) state variables at altitudes of 200 km and less. In particular we focus on the estimation of Pederson and Hall conductances, equatorial electric potential, and neutral winds. We will characterize the current state of IDA4D and EMPIRE in terms of data available to be assimilated and geophysical conditions. We will discuss the accuracy expected for given conditions, current limitations, and to what degree data assimilative models can be used to advance our understanding of the low/mid latitude physics at 200 km and below. We will use three case study events to help elucidate the current capabilities of IDA4D and EMPIRE: The March, 2013 storm, the March 2015 storm, and the March 2014 time period, which was relatively quiet. In additional to the normal data sets ingested by IDA4D and EMPIRE we intend to ingest observation from the C/NOFS satellite including radio occultations, in-situ measurements of plasma density and ion drifts. We will also ingest other measurements related to electric fields from coherent scatter radars and magnetometers when available. Finally, we will ingest neutral wind measurements from FPI's. We will conclude with a discussion of the future of IT data assimilation algorithms, the advantages of coupling to first principle models, and what future satellite missions such as ICON, GOLD and COSMIC 2 will contribute to improved IT data assimilation estimates.

  19. Increase of the adhesion ability and display of a rumen fungal xylanase on the cell surface of Lactobacillus casei by using a listerial cell-wall-anchoring protein.

    Science.gov (United States)

    Hsueh, Hsiang-Yun; Yu, Bi; Liu, Chi-Te; Liu, Je-Ruei

    2014-02-01

    Lactobacillus, which has great adhesion ability to intestinal mucosa and is able to hydrolyse plant cell walls, can be used more efficiently as a feed additive. To increase the adhesion ability and display a fungal xylanase on the cell surface of Lactobacillus casei, the Listeria monocytogenes cell-wall-anchoring protein gene, mub, was introduced into L. casei ATCC 393 cells and used as a fusion partner to display the rumen fungal xylanase XynCDBFV on the cell surface of the transformed strains. The transformed strain L. casei pNZ-mub, which harboured mub gene, displayed recombinant Mub on its cell surface and showed greater adhesion ability to Caco-2 cells than the parental strain. The transformed strain L. casei pNZ-mub/xyn, which harboured mub-xynCDBFV fusion gene, acquired the capacity to break down oat spelt xylan and exhibited greater competition ability against the adhesion of L. monocytogenes to Caco-2 cells, in comparison with the parental strain. Mub has a potential to be used as a fusion partner to display heterologous proteins on the cell surface of Lactobacillus. Moreover, this is the first report of the successful display of xylanase on the cell surface of Lactobacillus. © 2013 Society of Chemical Industry.

  20. Potential of Electric Power Production from Microbial Fuel Cell (MFC in Evapotranspiration Reactor for Leachate Treatment Using Alocasia macrorrhiza Plant and Eleusine indica Grass

    Directory of Open Access Journals (Sweden)

    Zaman Badrus

    2018-01-01

    Full Text Available Microbial fuel cell is one of attractive electric power generator from nature bacterial activity. While, Evapotranspiration is one of the waste water treatment system which developed to eliminate biological weakness that utilize the natural evaporation process and bacterial activity on plant roots and plant media. This study aims to determine the potential of electrical energy from leachate treatment using evapotranspiration reactor. The study was conducted using local plant, namely Alocasia macrorrhiza and local grass, namely Eleusine Indica. The system was using horizontal MFC by placing the cathodes and anodes at different chamber (i.e. in the leachate reactor and reactor with plant media. Carbon plates was used for chatode-anodes material with size of 40 cm x 10 cm x1 cm. Electrical power production was measure by a digital multimeter for 30 days reactor operation. The result shows electric power production was fluctuated during reactor operation from all reactors. The electric power generated from each reactor was fluctuated, but from the reactor using Alocasia macrorrhiza plant reach to 70 μwatt average. From the reactor using Eleusine Indica grass was reached 60 μwatt average. Electric power production fluctuation is related to the bacterial growth pattern in the soil media and on the plant roots which undergo the adaptation process until the middle of the operational period and then in stable growth condition until the end of the reactor operation. The results indicate that the evapotranspiration reactor using Alocasia macrorrhiza plant was 60-95% higher electric power potential than using Eleusine Indica grass in short-term (30-day operation. Although, MFC system in evapotranspiration reactor system was one of potential system for renewable electric power generation.

  1. Involvement of cell surface TG2 in the aggregation of K562 cells triggered by gluten.

    Science.gov (United States)

    Feriotto, G; Calza, R; Bergamini, C M; Griffin, M; Wang, Z; Beninati, S; Ferretti, V; Marzola, E; Guerrini, R; Pagnoni, A; Cavazzini, A; Casciano, F; Mischiati, C

    2017-03-01

    Gluten-induced aggregation of K562 cells represents an in vitro model reproducing the early steps occurring in the small bowel of celiac patients exposed to gliadin. Despite the clear involvement of TG2 in the activation of the antigen-presenting cells, it is not yet clear in which compartment it occurs. Herein we study the calcium-dependent aggregation of these cells, using either cell-permeable or cell-impermeable TG2 inhibitors. Gluten induces efficient aggregation when calcium is absent in the extracellular environment, while TG2 inhibitors do not restore the full aggregating potential of gluten in the presence of calcium. These findings suggest that TG2 activity is not essential in the cellular aggregation mechanism. We demonstrate that gluten contacts the cells and provokes their aggregation through a mechanism involving the A-gliadin peptide 31-43. This peptide also activates the cell surface associated extracellular TG2 in the absence of calcium. Using a bioinformatics approach, we identify the possible docking sites of this peptide on the open and closed TG2 structures. Peptide docks with the closed TG2 structure near to the GTP/GDP site, by establishing molecular interactions with the same amino acids involved in stabilization of GTP binding. We suggest that it may occur through the displacement of GTP, switching the TG2 structure from the closed to the active open conformation. Furthermore, docking analysis shows peptide binding with the β-sandwich domain of the closed TG2 structure, suggesting that this region could be responsible for the different aggregating effects of gluten shown in the presence or absence of calcium. We deduce from these data a possible mechanism of action by which gluten makes contact with the cell surface, which could have possible implications in the celiac disease onset.

  2. Magnetic capture of polydopamine-encapsulated Hela cells for the analysis of cell surface proteins.

    Science.gov (United States)

    Liu, Yiying; Yan, Guoquan; Gao, Mingxia; Zhang, Xiangmin

    2017-10-16

    A novel method to characterize cell surface proteins and complexes has been developed. Polydopamine (PDA)-encapsulated Hela cells were prepared for plasma membrane proteome research. Since the PDA protection, the encapsulated cells could be maintained for more than two weeks. Amino groups functionalized magnetic nanoparticles were also used for cell capture by the reaction with the PDA coatings. Plasma membrane fragments were isolated and enriched with assistance of an external magnetic field after disruption of the coated cells by ultrasonic treatment. Plasma membrane proteins (PMPs) and complexes were well preserved on the fragments and identified by shot-gun proteomic analytical strategy. 385 PMPs and 1411 non-PMPs were identified using the method. 85.2% of these PMPs were lipid-raft associated proteins. Ingenuity Pathway Analysis was employed for bio-information extraction from the identified proteins. It was found that 653 non-PMPs had interactions with 140 PMPs. Among them, epidermal growth factor receptor and its complexes, and a series of important pathways including STAT3 pathway were observed. All these results demonstrated that the new approach is of great importance in applying to the research of physiological function and mechanism of the plasma membrane proteins. This work developed a novel strategy for the proteomic analysis of cell surface proteins. According to the results, 73.3% of total identified proteins were lipid-raft associated proteins, which imply that the proposed method is of great potential in the identification of lipid-raft associated proteins. In addition, a series of protein-protein interactions and pathways related to Hela cells were pointed out. All these results demonstrated that our proposed approach is of great importance and could well be applied to the physiological function and mechanism research of plasma membrane proteins. Copyright © 2017. Published by Elsevier B.V.

  3. MICROBIAL CELL-SURFACE HYDROPHOBICITY - THE INVOLVEMENT OF ELECTROSTATIC INTERACTIONS IN MICROBIAL ADHESION TO HYDROCARBONS (MATH)

    NARCIS (Netherlands)

    GEERTSEMADOORNBUSCH, GI; VANDERMEI, HC; BUSSCHER, HJ

    Microbial adhesion to hydrocarbons (MATH) is the most commonly used method to determine microbial cell surface hydrophobicity. Since, however, the assay is based on adhesion, it is questionable whether the results reflect only the cell surface hydrophobicity or an interplay of hydrophobicity and

  4. A reference guide to microbial cell surface hydrophobicity based on contact angles

    NARCIS (Netherlands)

    van der Mei, HC; Busscher, HJ; Bos, R.R.M.

    1998-01-01

    Acid-base interactions form the origin of the hydrophobicity of microbial cell-surfaces and can be quantitated from contact angle measurements on microbial lawns with water, formamide, methyleneiodide and/or alpha-bromonaphthalene. This review provides a reference guide to microbial cell surface

  5. Efficient isolation and quantitative proteomic analysis of cancer cell plasma membrane proteins for identification of metastasis-associated cell surface markers.

    Science.gov (United States)

    Lund, Rikke; Leth-Larsen, Rikke; Jensen, Ole N; Ditzel, Henrik J

    2009-06-01

    Cell surface membrane proteins are involved in central processes such as cell signaling, cell-cell interactions, ion and solute transport, and they seem to play a pivotal role in several steps of the metastatic process of cancer cells. The low abundance and hydrophobic nature of cell surface membrane proteins complicate their purification and identification by MS. We used two isogenic cell lines with opposite metastatic capabilities in nude mice to optimize cell surface membrane protein purification and to identify potential novel markers of metastatic cancer. The cell surface membrane proteins were isolated by centrifugation/ultracentrifugation steps, followed by membrane separation using a Percoll/sucrose density gradient. The gradient fractions containing the cell surface membrane proteins were identified by enzymatic assays. Stable isotope labeling of the proteome of the metastatic cell line by SILAC followed by mass spectrometry analysis enabled identification and quantification of proteins that were differentially expressed in the two cell lines. Dual stable isotopic labels ((13)C-arginine and (13)C-lysine) instead of a single label ((13)C-arginine) increased the percentage of proteins that could be quantified from 40 to 93%. Repeated LC-MS/MS analyses (3-4 times) of each sample increased the number of identified proteins by 60%. The use of Percoll/sucrose density separation allowed subfractionation of membranes leading to enrichment of membrane proteins (66%) and reduction from 33% to only 16% of protein from other membranous organelles such as endoplasmatic reticulum, Golgi, and mitochondria. In total, our optimized methods resulted in 1919 protein identifications (corresponding to 826 at similarity level 80% (SL80); 1145 (509 at SL80) were identified by two or more peptides of which 622 (300 at SL80) were membrane proteins. The quantitative proteomic analysis identified 16 cell surface proteins as potential markers of the ability of breast cancer cells to

  6. Ionisation potential theorem in the presence of the electric field: Assessment of range-separated functional in the reproduction of orbital and excitation energies.

    Science.gov (United States)

    Borpuzari, Manash Protim; Boruah, Abhijit; Kar, Rahul

    2016-04-28

    Recently, the range-separated density functionals have been reported to reproduce gas phase orbital and excitation energies with good accuracy. In this article, we have revisited the ionisation potential theorem in the presence of external electric field. Numerical results on six linear molecules are presented and the performance of the range-separated density functionals in reproducing highest occupied molecular orbital (HOMO) energies, LUMO energies, HOMO-LUMO gaps in the presence of the external electric field is assessed. In addition, valence and Rydberg excitation energies in the presence of the external electric field are presented. It is found that the range-separated density functionals reproduce orbital and excitation energies accurately in the presence of the electric field. Moreover, we have performed fractional occupation calculation using cubic spline equation and tried to explain the performance of the functional.

  7. Analytical and numerical solutions of the potential and electric field generated by different electrode arrays in a tumor tissue under electrotherapy.

    Science.gov (United States)

    Bergues Pupo, Ana E; Reyes, Juan Bory; Bergues Cabrales, Luis E; Bergues Cabrales, Jesús M

    2011-09-24

    Electrotherapy is a relatively well established and efficient method of tumor treatment. In this paper we focus on analytical and numerical calculations of the potential and electric field distributions inside a tumor tissue in a two-dimensional model (2D-model) generated by means of electrode arrays with shapes of different conic sections (ellipse, parabola and hyperbola). Analytical calculations of the potential and electric field distributions based on 2D-models for different electrode arrays are performed by solving the Laplace equation, meanwhile the numerical solution is solved by means of finite element method in two dimensions. Both analytical and numerical solutions reveal significant differences between the electric field distributions generated by electrode arrays with shapes of circle and different conic sections (elliptic, parabolic and hyperbolic). Electrode arrays with circular, elliptical and hyperbolic shapes have the advantage of concentrating the electric field lines in the tumor. The mathematical approach presented in this study provides a useful tool for the design of electrode arrays with different shapes of conic sections by means of the use of the unifying principle. At the same time, we verify the good correspondence between the analytical and numerical solutions for the potential and electric field distributions generated by the electrode array with different conic sections.

  8. Analytical and numerical solutions of the potential and electric field generated by different electrode arrays in a tumor tissue under electrotherapy

    Directory of Open Access Journals (Sweden)

    Bergues Cabrales Jesús M

    2011-09-01

    Full Text Available Abstract Background Electrotherapy is a relatively well established and efficient method of tumor treatment. In this paper we focus on analytical and numerical calculations of the potential and electric field distributions inside a tumor tissue in a two-dimensional model (2D-model generated by means of electrode arrays with shapes of different conic sections (ellipse, parabola and hyperbola. Methods Analytical calculations of the potential and electric field distributions based on 2D-models for different electrode arrays are performed by solving the Laplace equation, meanwhile the numerical solution is solved by means of finite element method in two dimensions. Results Both analytical and numerical solutions reveal significant differences between the electric field distributions generated by electrode arrays with shapes of circle and different conic sections (elliptic, parabolic and hyperbolic. Electrode arrays with circular, elliptical and hyperbolic shapes have the advantage of concentrating the electric field lines in the tumor. Conclusion The mathematical approach presented in this study provides a useful tool for the design of electrode arrays with different shapes of conic sections by means of the use of the unifying principle. At the same time, we verify the good correspondence between the analytical and numerical solutions for the potential and electric field distributions generated by the electrode array with different conic sections.

  9. Preliminary evaluation of potential NO/sub x/ control strategies for the electric power industry. Volume I. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Greenfield, S.M.; Peyton, T.O.; Anderson, G.E.; Attaway, L.D.; Hillyer, M.J.; MacBride, D.W.

    1978-03-01

    Flow Resources Corporation has conducted an investigation of strategies for the control of nitrogen oxides emitted by the electric utility industry's fossil fuel fired power plants. The study focused on assurance of meeting the current annual NO/sub 2/ primary National Ambient Air Quality Standard (100 ..mu..g/m/sup 3/) through the year 2000. It included four sets of nuclear/coal growth scenarios and three sets of assumed emission restrictions and centered on 20 ''critical'' Air Quality Control Regions (AQCR) chosen for their potential for being in NO/sub 2/ noncompliance during the study time period. A review was conducted of federal and state NO/sub x/ regulations and photochemical diffusion modeling (the latter done under subcontract by System Applications, Inc.) The principal conclusion of this study is that in most critical AQCRs, reduction of coal-fired power plant emissions below 0.3 lbs NO/sub x/10/sup 6/ Btu appears to offer little leverage for substantially further reducing annual ambient concentrations of NO/sub 2/. This result, however, varies between AQCRs, and may necessitate a more detailed evaluation of control strategies.

  10. Three-dimensional needle-tip localization by electric field potential and camera hybridization for needle electromyography exam robotic simulator.

    Science.gov (United States)

    He, Siyu; Gomez-Tames, Jose; Yu, Wenwei

    2016-01-01

    As one of neurological tests, needle electromygraphy exam (NEE) plays an important role to evaluate the conditions of nerves and muscles. Neurology interns and novice medical staff need repetitive training to improve their skills in performing the exam. However, no training systems are able to reproduce multiple pathological conditions to simulate real needle electromyogram exam. For the development of a robotic simulator, three components need to be realized: physical modeling of upper limb morphological features, position-dependent electromyogram generation, and needle localization; the latter is the focus of this study. Our idea is to couple two types of sensing mechanism in order to acquire the needle-tip position with high accuracy. One is to segment the needle from camera images and calculate its insertion point on the skin surface by a top-hat transform algorithm. The other is voltage-based depth measurement, in which a conductive tissue-like phantom was used to realize both needle-tip localization and physical sense of needle insertion. For that, a pair of electrodes was designed to generate a near-linear voltage distribution along the depth direction of the tissue-like phantom. The accuracy of the needle-tip position was investigated by the electric field potential and camera hybridization. The results showed that the needle tip could be detected with an accuracy of 1.05±0.57 mm.

  11. Projected Demand and Potential Impacts to the National Airspace System of Autonomous, Electric, On-Demand Small Aircraft

    Science.gov (United States)

    Smith, Jeremy C.; Viken, Jeffrey K.; Guerreiro, Nelson M.; Dollyhigh, Samuel M.; Fenbert, James W.; Hartman, Christopher L.; Kwa, Teck-Seng; Moore, Mark D.

    2012-01-01

    Electric propulsion and autonomy are technology frontiers that offer tremendous potential to achieve low operating costs for small-aircraft. Such technologies enable simple and safe to operate vehicles that could dramatically improve regional transportation accessibility and speed through point-to-point operations. This analysis develops an understanding of the potential traffic volume and National Airspace System (NAS) capacity for small on-demand aircraft operations. Future demand projections use the Transportation Systems Analysis Model (TSAM), a tool suite developed by NASA and the Transportation Laboratory of Virginia Polytechnic Institute. Demand projections from TSAM contain the mode of travel, number of trips and geographic distribution of trips. For this study, the mode of travel can be commercial aircraft, automobile and on-demand aircraft. NASA's Airspace Concept Evaluation System (ACES) is used to assess NAS impact. This simulation takes a schedule that includes all flights: commercial passenger and cargo; conventional General Aviation and on-demand small aircraft, and operates them in the simulated NAS. The results of this analysis projects very large trip numbers for an on-demand air transportation system competitive with automobiles in cost per passenger mile. The significance is this type of air transportation can enhance mobility for communities that currently lack access to commercial air transportation. Another significant finding is that the large numbers of operations can have an impact on the current NAS infrastructure used by commercial airlines and cargo operators, even if on-demand traffic does not use the 28 airports in the Continental U.S. designated as large hubs by the FAA. Some smaller airports will experience greater demand than their current capacity allows and will require upgrading. In addition, in future years as demand grows and vehicle performance improves other non-conventional facilities such as short runways incorporated into

  12. Molecular Tension Probes for Imaging Forces at the Cell Surface.

    Science.gov (United States)

    Liu, Yang; Galior, Kornelia; Ma, Victor Pui-Yan; Salaita, Khalid

    2017-11-21

    composed of an extendable linker, such as polymer, oligonucleotide, or protein, and flanked by a fluorophore and quencher. By measuring the fluorescence emission of immobilized MTFM probes, one can infer the extension of the linker and the externally applied force. Thus, MTFM combines aspects of TFM and SMFS to optically report receptor forces across the entire cell surface with pN sensitivity. Specifically, we provide an in-depth review of MTFM probe design, which includes the extendable "spring", spectroscopic ruler, surface immobilization chemistry, and ligand design strategies. We also demonstrate the strengths and weaknesses of different versions of MTFM probes by discussing case studies involving the pN forces involved in epidermal growth factor receptor, integrin, and T-cell receptor signaling pathways. Lastly, we present a brief future outlook, primarily from a chemists' perspective, on the challenges and opportunities for the design of next generation MTFM probes.

  13. Structural changes in single membranes in response to an applied transmembrane electric potential revealed by time-resolved neutron/X-ray interferometry

    Energy Technology Data Exchange (ETDEWEB)

    Tronin, A.; Chen, C.-H.; Gupta, S. [Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104 (United States); Worcester, D. [Department of Physiology and Biophysics, University of California, Irvine, CA 92697 (United States); Biology Division, University of Missouri, Columbia, MO 65211 (United States); Lauter, V. [Spallation Neutron Source, Neutron Science Directorate, Oak Ridge National Laboratory, Oak Ridge, TN 37831 (United States); Strzalka, J.; Kuzmenko, I. [Advanced Photon Source, X-ray Science Division, Argonne National Laboratory, Argonne, IL 60439 (United States); Blasie, J.K., E-mail: jkblasie@sas.upenn.edu [Department of Chemistry, University of Pennsylvania, Philadelphia, PA 19104 (United States)

    2013-08-30

    Highlights: ► Time-resolved (or transient) neutron/X-ray reflectivity. ► Neutron/X-ray reflectivity enhanced by interferometric techniques. ► Electric potential induced changes in a hybrid lipid bilayer membrane. ► Electric potential induced changes in a voltage-sensor protein membrane. - Abstract: The profile structure of a hybrid lipid bilayer, tethered to the surface of an inorganic substrate and fully hydrated with a bulk aqueous medium in an electrochemical cell, was investigated as a function of the applied transbilayer electric potential via time-resolved neutron reflectivity, enhanced by interferometry. Significant, and fully reversible structural changes were observed in the distal half (with respect to the substrate surface) of the hybrid bilayer comprised of a zwitterionic phospholipid in response to a +100 mV potential with respect to 0 mV. These arise presumably due to reorientation of the electric dipole present in the polar headgroup of the phospholipid and its resulting effect on the thickness of the phospholipid’s hydrocarbon chain layer within the hybrid bilayer’s profile structure. The profile structure of the voltage-sensor domain from a voltage-gated ion channel protein within a phospholipid bilayer membrane, tethered to the surface of an inorganic substrate and fully hydrated with a bulk aqueous medium in an electrochemical cell, was also investigated as a function of the applied transmembrane electric potential via time-resolved X-ray reflectivity, enhanced by interferometry. Significant, fully-reversible, and different structural changes in the protein were detected in response to ±100 mV potentials with respect to 0 mV. The approach employed is that typical of transient spectroscopy, shown here to be applicable to both neutron and X-ray reflectivity of thin films.

  14. Elektromobility and electrical vehicles. Economic evaluation of the market potential in the year 2020; Elektromobilitaet und Elektrofahrzeuge. Oekonomische Bewertung des Marktpotenzials im Jahr 2020

    Energy Technology Data Exchange (ETDEWEB)

    Schuler, Bastian

    2011-07-01

    The author of the contribution under consideration reports on an economic evaluation of the market potential of electrically powered vehicles in the year 2020. Battery powered vehicles are analyzed from a macroeconomic and microeconomic point of view. Sixteens scenarios are considered in order to present a broadest possible context for the profitability analysis.

  15. Development of stress ulcers assessed by gastric electrical potential difference, pH of gastric juice, and endoscopy in patients in the intensive care unit

    DEFF Research Database (Denmark)

    Rubinstein, E; Gjørup, I; Schulze, S

    1992-01-01

    OBJECTIVE: To assess measurement of gastric electrical potential difference, pH of gastric mucosa, and endoscopic findings in patients in intensive care units who are at risk of developing stress ulcers. DESIGN: Open comparison with age- and sex-matched control subjects. SETTING: Herlev Hospital,...

  16. Asymmetric distribution of the ionospheric electric potential in the opposite hemispheres as inferred from the SuperDARN observations and FAC-based convection model

    DEFF Research Database (Denmark)

    Lukianova, R.; Hanuise, C.; Christiansen, Freddy

    2008-01-01

    We compare the SuperDARN convection patterns with the predictions of a new numerical model of the global distribution of ionospheric electric potentials. The model utilizes high-precision statistical maps of field-aligned currents (FAC) derived from measurements made by polar-orbiting low-altitud...

  17. Normative findings of electrically evoked compound action potential measurements using the neural response telemetry of the Nucleus CI24M cochlear implant system.

    NARCIS (Netherlands)

    Cafarelli-Dees, D.; Dillier, N.; Lai, W.K.; Wallenberg, E. von; Dijk, B. van; Akdas, F.; Aksit, M.; Batman, C.; Beynon, A.J.; Burdo, S.; Chanal, J.M.; Collet, L.; Conway, M.; Coudert, C.; Craddock, L.; Cullington, H.; Deggouj, N.; Fraysse, B.; Grabel, S.; Kiefer, J.; Kiss, J.G.; Lenarz, T.; Mair, A.; Maune, S.; Muller-Deile, J.; Piron, J.P.; Razza, S.; Tasche, C.; Thai-Van, H.; Toth, F.; Truy, E.; Uziel, A.; Smoorenburg, G.F.

    2005-01-01

    One hundred and forty-seven adult recipients of the Nucleus 24 cochlear implant system, from 13 different European countries, were tested using neural response telemetry to measure the electrically evoked compound action potential (ECAP), according to a standardised postoperative measurement

  18. Investigation of the Optimum Farming Temperature for Grifola frondosa and Growth Promotion using the Bio-Electric Potential as an Index

    Science.gov (United States)

    Yanagibashi, Hideyuki; Hirama, Junji; Matsuda, Masato; Miyamoto, Toshio

    The purpose of this study was to investigate the optimum farming conditions for mushrooms from the view point of engineering field. As the bio-electric potential of mushrooms is considered to be closely related to the activation of mushroom cells, this relationship has been used to analyze the dependence of the morphogenetic characteristics of Grifola frondosa on farming temperatures (from 16 to 22 degree C). The experimental results indicated that a maximum response was exhibited, with correspondingly favorable morphogenesis obtained at 18 degree C. Based on the experimental results, including those in a previous study, it was assumed that the larger the bio-electric potential becomes, the higher the growth yield reaches. In order to support this assumption, growth promotion was conducted by intentionally activating the bio-electric potential within the mushrooms by stimulating them with short bursts of illumination. The resulting observation of growth promotion permitted the conclusion that the bio-electric potential can, indeed, be regarded as an index of growth.

  19. Functional Homomers and Heteromers of Dopamine D2L and D3 Receptors Co-exist at the Cell Surface

    Science.gov (United States)

    Pou, Chantevy; Mannoury la Cour, Clotilde; Stoddart, Leigh A.; Millan, Mark J.; Milligan, Graeme

    2012-01-01

    Human dopamine D2long and D3 receptors were modified by N-terminal addition of SNAP or CLIP forms of O6-alkylguanine-DNA-alkyltransferase plus a peptide epitope tag. Cells able to express each of these four constructs only upon addition of an antibiotic were established and used to confirm regulated and inducible control of expression, the specificity of SNAP and CLIP tag covalent labeling reagents, and based on homogenous time-resolved fluorescence resonance energy transfer, the presence of cell surface D2long and D3 receptor homomers. Following constitutive expression of reciprocal constructs, potentially capable of forming and reporting the presence of cell surface D2long-D3 heteromers, individual clones were assessed for levels of expression of the constitutively expressed protomer. This was unaffected by induction of the partner protomer and the level of expression of the partner required to generate detectable cell surface D2long–D3 heteromers was defined. Such homomers and heteromers were found to co-exist and using a reconstitution of function approach both homomers and heteromers of D2long and D3 receptors were shown to be functional, potentially via trans-activation of associated G protein. These studies demonstrate the ability of dopamine D2long and D3 receptors to form both homomers and heteromers, and show that in cells expressing each subtype a complex mixture of homomers and heteromers co-exists at steady state. These data are of potential importance both to disorders in which D2long and D3 receptors are implicated, like schizophrenia and Parkinson disease, and also to drugs exerting their actions via these sites. PMID:22291025

  20. Substance P Increases Cell-Surface Expression of CD74 (Receptor for Macrophage Migration Inhibitory Factor: In Vivo Biotinylation of Urothelial Cell-Surface Proteins

    Directory of Open Access Journals (Sweden)

    Katherine L. Meyer-Siegler

    2009-01-01

    N-hydroxysulfosuccinimide biotin ester-labeled surface urothelial proteins in rats treated either with saline or substance P (SP, 40 μg/kg. The bladder was examined by histology and confocal microscopy. Biotinylated proteins were purified by avidin agarose, immunoprecipitated with anti-MIF or anti-CD74 antibodies, and detected with strepavidin-HRP. Only superficial urothelial cells were biotinylated. These cells contained a biotinylated MIF/CD74 cell-surface complex that was increased in SP-treated animals. SP treatment increased MIF and CD74 mRNA in urothelial cells. Our data indicate that intraluminal MIF, released from urothelial cells as a consequence of SP treatment, interacts with urothelial cell-surface CD74. These results document that our previously described MIF-CD74 interaction occurs at the urothelial cell surface.

  1. Helicobacter pylori usurps cell polarity to turn the cell surface into a replicative niche.

    Directory of Open Access Journals (Sweden)

    Shumin Tan

    2009-05-01

    Full Text Available Helicobacter pylori (Hp intimately interacts with the gastric epithelial surface and translocates the virulence factor CagA into host cells in a contact-dependent manner. To study how Hp benefits from interacting with the cell surface, we developed live-cell microscopy methods to follow the fate of individual bacteria on the cell surface and find that Hp is able to replicate and form microcolonies directly over the intercellular junctions. On polarized epithelia, Hp is able to grow directly on the apical cell surface in conditions that do not support the growth of free-swimming bacteria. In contrast, mutants in CagA delivery are defective in colonization of the apical cell surface. Hp perturbs the polarized epithelium in a highly localized manner, since wild-type Hp does not rescue the growth defect of the CagA-deficient mutants upon co-infection. CagA's ability to disrupt host cell polarity is a key factor in enabling colonization of the apical cell surface by Hp, as disruption of the atypical protein kinase C/Par1b polarity pathway leads to rescue of the mutant growth defect during apical infection, and CagA-deficient mutants are able to colonize the polarized epithelium when given access to the basolateral cell surface. Our study establishes the cell surface as a replicative niche and the importance of CagA and its effects on host cell polarity for this purpose.

  2. Ketone isosteres of 2-N-acetamidosugars as substrates for metabolic cell surface engineering

    Energy Technology Data Exchange (ETDEWEB)

    Hang, Howard C.; Bertozzi, Carolyn R.

    2000-08-22

    Novel chemical reactivity can be engendered on cell surfaces by the metabolic incorporation of unnatural sugars into cell surface glycoconjuagtes. 2-N-Acetamido sugars such as GalNAc and GlcNAc are abundant components of cell surface glycoconjugates, and hence attractive targets for metabolic cell surface engineering. Here we report (1) the synthesis of isosteric analogs bearing a ketone group in place of the N-acetamido group, and (2) evaluation of their metabolic incorporation into mammalian cell surface glycans. A ketone isostere of GalNAc was metabolized by CHO cells through the salvage pathway and delivered to O-linked glycoproteins on the cell surface. Its residence at the core position of O-linked glycans is suggested by studies with a-benzyl GalNAc, an inhibitor of O-linked oligosaccharide extension. A mutant CHO cell line lacking endogenous UDP-GalNAc demonstrated enhanced metabolism of the GalNAc analog, suggesting that competition with native intermediates might limits enzymatic transformation in mammalian cells. A ketone isostere of GlcNAc could not be detected on CHO or human cell surfaces after incubation. Thus, the enzymes in the GlcNAc salvage pathway might be less permissive of unnatural substrates than those comprising the GalNAc salvage pathway. Alternatively, high levels of endogenous GlcNAc derivatives might compete with the ketone isostere and prevent its incorporation into oligosaccharides.

  3. Polypyrrole/Alginate Hybrid Hydrogels: Electrically Conductive and Soft Biomaterials for Human Mesenchymal Stem Cell Culture and Potential Neural Tissue Engineering Applications.

    Science.gov (United States)

    Yang, Sumi; Jang, LindyK; Kim, Semin; Yang, Jongcheol; Yang, Kisuk; Cho, Seung-Woo; Lee, Jae Young

    2016-11-01

    Electrically conductive biomaterials that can efficiently deliver electrical signals to cells or improve electrical communication among cells have received considerable attention for potential tissue engineering applications. Conductive hydrogels are desirable particularly for neural applications, as they can provide electrical signals and soft microenvironments that can mimic native nerve tissues. In this study, conductive and soft polypyrrole/alginate (PPy/Alg) hydrogels are developed by chemically polymerizing PPy within ionically cross-linked alginate hydrogel networks. The synthesized hydrogels exhibit a Young's modulus of 20-200 kPa. Electrical conductance of the PPy/Alg hydrogels could be enhanced by more than one order of magnitude compared to that of pristine alginate hydrogels. In vitro studies with human bone marrow-derived mesenchymal stem cells (hMSCs) reveal that cell adhesion and growth are promoted on the PPy/Alg hydrogels. Additionally, the PPy/Alg hydrogels support and greatly enhance the expression of neural differentiation markers (i.e., Tuj1 and MAP2) of hMSCs compared to tissue culture plate controls. Subcutaneous implantation of the hydrogels for eight weeks induces mild inflammatory reactions. These soft and conductive hydrogels will serve as a useful platform to study the effects of electrical and mechanical signals on stem cells and/or neural cells and to develop multifunctional neural tissue engineering scaffolds. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  4. OPTUM. Optimization of the potentials of environmental relief of electric-powered vehicles. Integrated view of vehicle utilization and energy industry; OPTUM. Optimierung der Umweltentlastungspotenziale von Elektrofahrzeugen. Integrierte Betrachtung von Fahrzeugnutzung und Energiewirtschaft. Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Zimmer, Wiebke; Buchert, Matthias; Dittrich, Stefanie [Oeko-Institut e.V., Berlin (DE)] (and others)

    2011-10-15

    Germany wants to reduce its greenhouse gas emissions by 40% up to 2020 compared to 1990. Although today's vehicles with combustion engines are more efficient than 15 years ago, still too much carbon dioxide is released. Under this aspect, the Federal Government promotes the electric mobility in a comprehensive manner. As part of the research project OPTUM (environmental relief potential of electric-powered vehicles - Integrated consideration of vehicle use and energy industry) an integrative approach to account for the potential environmental benefits of electric-powered vehicles was tracked. This research project has the following main contents: (a) Acceptance and attractiveness of electric-powered vehicles; (b) Market potential for electric vehicles; (c) Interaction with the electricity market; (d) Potentials of reduction of CO{sub 2} emissions of electric-powered vehicles; (e) Economic analysis of storage media; (f) Resource efficiency of the system of electromobility.

  5. SOTEA, a software tool for ascertaining the efficiency potential of electrical drives - Final report; SOTEA, Softwaretool zur Ermittlung des Effizienzpotenzials bei elektrischen Antrieben - Schlussbericht

    Energy Technology Data Exchange (ETDEWEB)

    Brunner, C. U. [S.A.F.E. Schweizerische Agentur fuer Energieeffizienz, Zuerich (Switzerland); Heldstab, T. [hematik, Heldstab Systemoptimierung und Informatik, Zuerich (Switzerland)

    2009-08-15

    As a scientific base for the Swiss electric motor efficiency implementation program Topmotors a software tool for industry managers has been developed and tested. The software allows an energy efficiency engineer in a first contact with industrial managers and with few simple data on the plant operation to estimate the energy efficiency potential of electric motor systems including pay-back and investment. The data can be fed to a laptop computer on site and the results can be shown immediately. The software has been programmed and tested with five prime users. The generally positive reactions were evaluated and the tool subsequently improved. 11 industrial objects with a total of 77.6 GWh electricity consumption and 7.9 million CHF electricity cost were studied. The SOTEA estimate is an annual efficiency improvement of the electric motor systems of 6.9 GWh (11 % of electricity for motors) with an average pay-back time of 1.7 years. The SOTEA software tool is publicly available since September 2008 under www.topmotors.ch, from 1 April 2009 in a Beta-2b version. It has been downloaded until 28 June 2009 218 times by 132 persons. It will be improved with results from new pilot studies. To avoid problems with different update versions a direct internet solution will be studied. The program will also be made available internationally for English speaking users for the IEA 4E EMSA project: International Energy Agency, Implementing Agreement for Efficient Electrical End-Use Equipment, Electric Motor Systems Annex www.motorsystems.org. (authors)

  6. Three-dimensional needle-tip localization by electric field potential and camera hybridization for needle electromyography exam robotic simulator

    Directory of Open Access Journals (Sweden)

    He SY

    2016-06-01

    Full Text Available Siyu He,1 Jose Gomez-Tames,1 Wenwei Yu1,2 1Medical System Engineering Department, Graduate School of Engineering, 2Center for Frontier Medical Engineering, Chiba University, Chiba, Japan Abstract: As one of neurological tests, needle electromygraphy exam (NEE plays an important role to evaluate the conditions of nerves and muscles. Neurology interns and novice medical staff need repetitive training to improve their skills in performing the exam. However, no training systems are able to reproduce multiple pathological conditions to simulate real needle electromyogram exam. For the development of a robotic simulator, three components need to be realized: physical modeling of upper limb morphological features, position-dependent electromyogram generation, and needle localization; the latter is the focus of this study. Our idea is to couple two types of sensing mechanism in order to acquire the needle-tip position with high accuracy. One is to segment the needle from camera images and calculate its insertion point on the skin surface by a top-hat transform algorithm. The other is voltage-based depth measurement, in which a conductive tissue-like phantom was used to realize both needle-tip localization and physical sense of needle insertion. For that, a pair of electrodes was designed to generate a near-linear voltage distribution along the depth direction of the tissue-like phantom. The accuracy of the needle-tip position was investigated by the electric field potential and camera hybridization. The results showed that the needle tip could be detected with an accuracy of 1.05±0.57 mm. Keywords: needle-tip localization, needle EMG exam, top-hat transform, tissue-like phantom, voltage distribution simulation

  7. Angiogenic potential of endothelial and tumor cells seeded on gelatin-based hydrogels in response to electrical stimulations.

    Science.gov (United States)

    Tzoneva, Rumiana; Uzunova, Veselina; Apostolova, Sonia; Krüger-Genge, Anne; Neffe, Axel T; Jung, Friedrich; Lendlein, Andreas

    2016-01-01

    Angiogenesis is one of the key processes during development, wound healing and tumor formation. Prerequisite for its existence is the presence of endogenous electrical fields (EFs) generated by active ion transport across polarized epithelia and endothelia, and appearance of the transcellular potentials. During angiogenesis cellular factor as endothelial growth factor (VEGF), synthesis of adhesive proteins and membrane metalloproteinases (MMPs) govern the angiogenic response to different external stimuli as biomaterials interactions and/or exogenous EF. Gelatin-based hydrogels with elasticities comparable to human tissues have shown to influence cell behavior as well as cell attachment, protein synthesis, VEGF and MMP's production after the application of EF. Gelatin-based matrices with 3 (G10_LNCO3), 5 (G10_LNCO5), and 8 (G10_LNCO8) fold excess of isocyanate groups per mol of amine groups present in gelatin were used. Human umbilical endothelial cells (HUVEC) (Lonza Basel, Switzerland) and highly invasive breast cancer MDA-MB-231 cells (ATCC®HTB-26TM) were used. For an estimation of the amount of VEGF released from cells a commercially available VEGF ELISA (Thermo Fisher Scientific, Germany) kit was used. Fibronectin (FN) enzyme immunoassay (EIA) was used to analyze the secreted amount of FN by cells seeded on the materials. Secreted MMPs were analyzed by zymography. Gelatin-based hydrogels attracted HUVEC adhesion and diminished the adhesion of MDA-MB-231 cells. The applied direct current (DC) EF induced an almost 5-fold increase in VEGF production by HUVEC seeded on gelatin-based hydrogels, while in contrast, the applied EF decreased the production of VEGF by cancer cells. FN synthesis was elevated in HUVEC cells seeded on gelatin-based materials in comparison to FN synthesis by cancer cells. HUVEC seeded on gelatin hydrogels showed an expression mainly of MMP-2. The application of EF increased the production of MMP-2 in HUVEC seeded on gelatin materials. In

  8. Heat-Related Mortality in Japan after the 2011 Fukushima Disaster: An Analysis of Potential Influence of Reduced Electricity Consumption.

    Science.gov (United States)

    Kim, Yoonhee; Gasparrini, Antonio; Hashizume, Masahiro; Honda, Yasushi; Ng, Chris Fook Sheng; Armstrong, Ben

    2017-07-06

    In March 2011, the Great East Japan Earthquake devastated several power stations and caused severe electricity shortages. This accident was followed by the implementation of policies to reduce summer electricity consumption in the affected areas, for example, by limiting air-conditioning (AC) use. This provided a natural experimental scenario to investigate if these policies were associated with an increase in heat-related mortality. We examined whether the reduced electricity consumption in warm season modified heat-related mortality from 2008 to 2012. We conducted prefecture-specific interrupted time-series (ITS) analyses to compare temperature-mortality associations before and after the earthquake, and used meta-analysis to generate combined effect estimates for the most affected and less affected areas (prefectures with >10% or ≤10% reductions in electricity consumption, respectively). We then examined whether the temperature-mortality association in Tokyo, one of the most affected areas, was modified by the percent reduction in electricity consumption relative to expected consumption for comparable days before the earthquake. Contrary to expectations, we estimated a 5-9% reduction in all-cause heat-related mortality after the earthquake in the 15 prefectures with the greatest reduction in electricity consumption, and little change in the other prefectures. However, the percent reduction in observed vs. expected daily electricity consumption after the earthquake did not significantly modify daily heat-related mortality in Tokyo. In the prefectures with the greatest reductions in electricity consumption, heat-related mortality decreased rather than increased following the Great East Japan Earthquake. Additional research is needed to determine whether this finding holds for other populations and regions, and to clarify its implications for policies to reduce the consequences of climate change on health. https://doi.org/10.1289/EHP493.

  9. Micro-Cogeneration Incl. The Conversion of Chemical Energy of Biomass to Electric Energy and the Low Potential Heat

    Science.gov (United States)

    Huzvar, Jozef; Kapjor, Andrej

    2011-06-01

    This article deals with combined production of heat and electricity for small premises, such as households, where energy consumption is around few kilowatts. This proposal of micro co-generation unit uses as a heat source an automatic burner for combustion of wood pellets. Construction of an equipment for the heat transport can be designed using different basic ways of heat transfer. Electricity is produced by the two-stroke steam engine and the generator.

  10. Smart use of storage potentials of electric vehicles for renewable energy generation in the built environment: A design scenario

    OpenAIRE

    Van Timmeren, A.; Bauer, T.C.; Silvester, S.

    2011-01-01

    In this paper, results are reported of a technology assessment of use of electrical vehicles for energy storage (of renewable sources), their integration in the built environment and attached required power and charging systems for the Netherlands. This was done as part of the DIEMIGO project on integration of electrical mobility in the built environment. Around the world several concepts for EV charging and EV/Building interfaces have been developed, or are under development. In general howe...

  11. Survey of surface proteins from the pathogenic Mycoplasma hyopneumoniae strain 7448 using a biotin cell surface labeling approach.

    Directory of Open Access Journals (Sweden)

    Luciano Antonio Reolon

    Full Text Available The characterization of the repertoire of proteins exposed on the cell surface by Mycoplasma hyopneumoniae (M. hyopneumoniae, the etiological agent of enzootic pneumonia in pigs, is critical to understand physiological processes associated with bacterial infection capacity, survival and pathogenesis. Previous in silico studies predicted that about a third of the genes in the M. hyopneumoniae genome code for surface proteins, but so far, just a few of them have experimental confirmation of their expression and surface localization. In this work, M. hyopneumoniae surface proteins were labeled in intact cells with biotin, and affinity-captured biotin-labeled proteins were identified by a gel-based liquid chromatography-tandem mass spectrometry approach. A total of 20 gel slices were separately analyzed by mass spectrometry, resulting in 165 protein identifications corresponding to 59 different protein species. The identified surface exposed proteins better defined the set of M. hyopneumoniae proteins exposed to the host and added confidence to in silico predictions. Several proteins potentially related to pathogenesis, were identified, including known adhesins and also hypothetical proteins with adhesin-like topologies, consisting of a transmembrane helix and a large tail exposed at the cell surface. The results provided a better picture of the M. hyopneumoniae cell surface that will help in the understanding of processes important for bacterial pathogenesis. Considering the experimental demonstration of surface exposure, adhesion-like topology predictions and absence of orthologs in the closely related, non-pathogenic species Mycoplasma flocculare, several proteins could be proposed as potential targets for the development of drugs, vaccines and/or immunodiagnostic tests for enzootic pneumonia.

  12. Attractivity and acceptance of electric-powered vehicles. Results of the project OPTUM - Optimisation of the potentials of environmental reliefs of electic-powered vehicles; Attraktivitaet und Akzeptanz von Elektroautos. Ergebnisse aus dem Projekt OPTUM - Optimierung der Umweltentlastungspotenziale von Elektrofahrzeugen

    Energy Technology Data Exchange (ETDEWEB)

    Goetz, Konrad; Sunderer, Georg; Birzle-Harder, Barbara; Deffner, Jutta

    2012-07-01

    In the research project OPTUM it was analysed to what extent environmental reliefs can be gained by electric cars. For this purpose an integrated approach was pursued, taking into account both considerations on the vehicle side and interactions with the energy sector. In detail the following key questions were analysed: acceptance and attractiveness of electric vehicles, market potentials for electric vehicles, interactions with the energy sector, CO2 reduction potentials for electric mobility, economic analysis of the storage media and resource efficiency of electric mobility. In this report the results concerning the question of acceptance and attractiveness of electric vehicles are presented. In doing so, results of two empirical investigations are described which were carried out in OPTUM to determine the acceptance and attractiveness of electric vehicles. The first one is a qualitative analysis by means of focus group discussions; the second one a standardised survey among buyers of new cars. The standardised survey contained a conjoint analysis, in which the participants had to choose between conventional cars, plug-in-hybrid vehicles and all-electrical cars. The empirical analyses show: There is an enormous potential of acceptance for electric cars (plug-in-hybrids and all-electric vehicles). Depending on scenario and vehicle class, especially for all-electrical cars, a potential of acceptance exists from 12 to 25 percent. Furthermore, both empirical investigations provide indications how the potential of acceptance can be realised or even extended.

  13. Electricity Transmission, Pipelines, and National Trails: An Analysis of Current and Potential Intersections on Federal Lands in the Eastern United States, Alaska, and Hawaii

    Energy Technology Data Exchange (ETDEWEB)

    Kuiper, James A. [Argonne National Lab. (ANL), Argonne, IL (United States); Krummel, John R. [Argonne National Lab. (ANL), Argonne, IL (United States); Hlava, Kevin J. [Argonne National Lab. (ANL), Argonne, IL (United States); Moore, H. Robert [Argonne National Lab. (ANL), Argonne, IL (United States); Orr, Andrew B. [Argonne National Lab. (ANL), Argonne, IL (United States); Schlueter, Scott O. [Argonne National Lab. (ANL), Argonne, IL (United States); Sullivan, Robert G. [Argonne National Lab. (ANL), Argonne, IL (United States); Zvolanek, Emily A. [Argonne National Lab. (ANL), Argonne, IL (United States)

    2016-11-21

    As has been noted in many reports and publications, acquiring new or expanded rights-of-way for transmission is a challenging process, because numerous land use and land ownership constraints must be overcome to develop pathways suitable for energy transmission infrastructure. In the eastern U.S., more than twenty federally protected national trails (some of which are thousands of miles long, and cross many states) pose a potential obstacle to the development of new or expanded electricity transmission capacity. However, the scope of this potential problem is not well-documented, and there is no baseline information available that could allow all stakeholders to study routing scenarios that could mitigate impacts on national trails. This report, Electricity Transmission, Pipelines, and National Trails: An Analysis of Current and Potential Intersections on Federal Lands in the Eastern United States, was prepared by the Environmental Science Division of Argonne National Laboratory (Argonne). Argonne was tasked by DOE to analyze the “footprint” of the current network of National Historic and Scenic Trails and the electricity transmission system in the 37 eastern contiguous states, Alaska, and Hawaii; assess the extent to which national trails are affected by electrical transmission; and investigate the extent to which national trails and other sensitive land use types may be affected in the near future by planned transmission lines. Pipelines are secondary to transmission lines for analysis, but are also within the analysis scope in connection with the overall directives of Section 368 of the Energy Policy Act of 2005, and because of the potential for electrical transmission lines being collocated with pipelines.

  14. Microbial cell surface characteristics: Elucidating attachment/detachment using hydrophobicity and electrokinetic measurements

    Science.gov (United States)

    The surface properties of microorganisms play an important role in their behavior within the environment. Electrophoretic mobility and cell surface hydrophobicity of bacterial cells influence their initial interaction with surfaces and mediate their stability within an aqueous su...

  15. Time-kill profiles and cell-surface morphological effects of crude ...

    African Journals Online (AJOL)

    MK1201 mycelial extract on the viability and cell surface morphology of methicillin-susceptible Staphylococcus aureus (MSSA) and methicillin-resistant Staphylococcus aureus (MRSA). Methods: Time-kill assays were conducted by incubating test ...

  16. Regulation of ADAM12 cell-surface expression by protein kinase C epsilon

    DEFF Research Database (Denmark)

    Sundberg, Christina; Thodeti, Charles Kumar; Kveiborg, Marie

    2004-01-01

    The ADAM (a disintegrin and metalloprotease) family consists of multidomain cell-surface proteins that have a major impact on cell behavior. These transmembrane-anchored proteins are synthesized as proforms that have (from the N terminus): a prodomain; a metalloprotease-, disintegrin...... as a constitutively active protein. However, little is known about the regulation of ADAM12 cell-surface translocation. Here, we used human RD rhabdomyosarcoma cells, which express ADAM12 at the cell surface, in a temporal pattern. We report that protein kinase C (PKC) epsilon induces ADAM12 translocation to the cell......-like-, cysteine-rich, epidermal growth factor-like, and transmembrane domain; and a cytoplasmic tail. The 90-kDa mature form of human ADAM12 is generated in the trans-Golgi through cleavage of the prodomain by a furin-peptidase and is stored intracellularly until translocation to the cell surface...

  17. Proteomic inventory of "anchorless" proteins on the colon adenocarcinoma cell surface.

    NARCIS (Netherlands)

    Tjalsma, H.; Pluk, W.J.G.; Heuvel, L.P.W.J. van den; Peters, W.H.M.; Roelofs, R.H.W.M.; Swinkels, D.W.

    2006-01-01

    Surface proteins play important pathophysiological roles in health and disease, and accumulating proteomics-based studies suggest that several "non-membrane" proteins are sorted to the cell surface by unconventional mechanisms. Importantly, these proteins may comprise attractive therapeutic targets

  18. Cell-surface glycosyltransferases in cultured fibroblasts: increased activity and release during serum stimulation of growth.

    Science.gov (United States)

    LaMont, J T; Gammon, M T; Isselbacher, K J

    1977-01-01

    Cell-surface galactosyltransferase was studied in suspensions of intact baby hamster kidney fibroblasts with both endogenous and exogenous glycoprotein acceptors. The cell-surface location of galactosyltransferase was demonstrated in experiments with the enzyme modifier alpha-lactalbumin, which does not enter the cell. The addition of alpha-lactalbumin to the assay medium for galactosyltransferase resulted in accumulation of lactose in the medium but not in the cells. There was no detectable hydrolysis of UDP-galactose to free galactose by these cells, nor did a 100-fold molar excess of free galactose inhibit cell-surface galactosyltransferase. There was a marked increase in specific activity of cell-surface exogenous galactosyltransferase in serum-stimulated as compared to resting fibroblasts. Dividing but not resting fibroblasts released galactosyltransferase, but not sialyl- or fucosyltransferase, in soluble form into the tissue culture medium. The release of galactosyltransferase was greater from virally transformed than from nontransformed fibroblasts. PMID:191828

  19. Redesigning of Microbial Cell Surface and Its Application to Whole-Cell Biocatalysis and Biosensors.

    Science.gov (United States)

    Han, Lei; Zhao, Yukun; Cui, Shan; Liang, Bo

    2017-11-22

    Microbial cell surface display technology can redesign cell surfaces with functional proteins and peptides to endow cells some unique features. Foreign peptides or proteins are transported out of cells and immobilized on cell surface by fusing with anchoring proteins, which is an effective solution to avoid substance transfer limitation, enzyme purification, and enzyme instability. As the most frequently used prokaryotic and eukaryotic protein surface display system, bacterial and yeast surface display systems have been widely applied in vaccine, biocatalysis, biosensor, bioadsorption, and polypeptide library screening. In this review of bacterial and yeast surface display systems, different cell surface display mechanisms and their applications in biocatalysis as well as biosensors are described with their strengths and shortcomings. In addition to single enzyme display systems, multi-enzyme co-display systems are presented here. Finally, future developments based on our and other previous reports are discussed.

  20. Distinct roles for dystroglycan, beta1 integrin and perlecan in cell surface laminin organization

    DEFF Research Database (Denmark)

    Henry, M D; Satz, J S; Brakebusch, C

    2001-01-01

    Dystroglycan (DG) is a cell surface receptor for several extracellular matrix (ECM) molecules including laminins, agrin and perlecan. Recent data indicate that DG function is required for the formation of basement membranes in early development and the organization of laminin on the cell surface....... Here we show that DG-mediated laminin clustering on mouse embryonic stem (ES) cells is a dynamic process in which clusters are consolidated over time into increasingly more complex structures. Utilizing various null-mutant ES cell lines, we define roles for other molecules in this process. In beta1...... integrin-deficient ES cells, laminin-1 binds to the cell surface, but fails to organize into more morphologically complex structures. This result indicates that beta1 integrin function is required after DG function in the cell surface-mediated laminin assembly process. In perlecan-deficient ES cells...

  1. Galactose-specific recognition system of mammalian liver: receptor distribution on the hepatocyte cell surface

    OpenAIRE

    1981-01-01

    An isolated perfused liver system was used to study the distribution of asialoglycoprotein (ASGP) binding sites on rat hepatocyte cell surfaces. The number of surface receptors was quantitated by monitoring clearance of 125I-labeled ligands from the perfusate medium under two conditions that blocked their internalization: low temperature (less than 5 degrees C) or brief formaldehyde fixation. The cell surface distribution of binding sites was visualized in the electron microscope with either ...

  2. The Mouse C2C12 Myoblast Cell Surface N-Linked Glycoproteome

    OpenAIRE

    Gundry, Rebekah L.; Raginski, Kimberly; Tarasova, Yelena; Tchernyshyov, Irina; Bausch-Fluck, Damaris; Elliott, Steven T.; Boheler, Kenneth R.; Van Eyk, Jennifer E.; Wollscheid, Bernd

    2009-01-01

    Endogenous regeneration and repair mechanisms are responsible for replacing dead and damaged cells to maintain or enhance tissue and organ function, and one of the best examples of endogenous repair mechanisms involves skeletal muscle. Although the molecular mechanisms that regulate the differentiation of satellite cells and myoblasts toward myofibers are not fully understood, cell surface proteins that sense and respond to their environment play an important role. The cell surface capturing ...

  3. Full Length Amelogenin Binds to Cell Surface LAMP-1 on Tooth Root/Periodontium Associated Cells

    Science.gov (United States)

    Zhang, Hai; Tompkins, Kevin; Garrigues, Jacques; Snead, Malcolm L.; Gibson, Carolyn W.; Somerman, Martha J.

    2010-01-01

    Objectives Lysosome-associated membrane protein-1 (LAMP-1) has been suggested to be a cell surface receptor for a specific amelogenin isoform, leucine-rich amelogenin peptide or LRAP. However, it is unclear if LAMP-1 is an amelogenin receptor for dental mesenchymal cells. The goal of this study was to determine if LAMP-1 serves as a cell surface binding site for full length amelogenin on tooth root/periodontium associated mesenchymal cells. Design Murine dental follicle cells and cementoblasts (OCCM-30) were cultured for 2 days followed by addition of full length recombinant mouse amelogenin, rp(H)M180. Dose-response (0 to 100 μg/ml) and time course (0 to 120 minutes) assays were performed to determine the optimal conditions for live cell surface binding using immuno-fluorescent microscopy. A competitive binding assay was performed to determine binding specificity by adding Emdogain (1 mg/ml) to the media. An antibody against LAMP-1 was used to detect the location of LAMP-1 on the cell surface and the pattern was compared to cell surface bound amelogenin. Both amelogenin and cell surface LAMP-1 were immuno-co-localized to compare the amount and distribution pattern. Results Maximum surface binding was achieved with 50 μg/ml of rp(H)M180 for 120 minutes. This binding was specific as demonstrated by competitive inhibition (79% lower) with the addition of Emdogain. The binding pattern for rp(H)M180 was similar to the distribution of surface LAMP-1 on dental follicle cells and cementoblasts. The high co-localization coefficient (0.92) for rp(H)M180 and LAMP-1 supports rp(H)M180 binding to cell surface LAMP-1. Conclusions The data from this study suggest that LAMP-1 can serve as a cell surface binding site for amelogenin on dental follicle cells and cementoblasts. PMID:20382373

  4. Cell surface amyloid proteins of microorganisms: structure, properties and significance in medicine

    OpenAIRE

    Rekstina V.V.; Gorkovskii A.A.; Bezsonov E.E.; Kalebina T.S.

    2016-01-01

    This review summarizes data which describe properties of microbial cell surface amyloids proteins. Definitions of amyloids and microbial functional amyloids are given. The review provides numerous examples of research in which the presence of amyloid-like properties in microbial cell surface proteins is demonstrated convincingly. Studies of the important role of pili, curli, tafi and some other bacterial fibrillar proteins in host colonization are reviewed. Data on amyloid proteins of yeast c...

  5. Role of lactobacillus cell surface hydrophobicity as probed by AMF in adhesion to surfaces at low and high ionic strength

    NARCIS (Netherlands)

    Vadillo-Rodriguez, V.; Busscher, H.J.; Meij, van der H.C.; Vries, de J.; Norde, W.

    2005-01-01

    The S-layer present at the outermost cell surface of some lactobacillus species is known to convey hydrophobicity to the lactobacillus cell surface. Yet, it is commonly found that adhesion of lactobacilli to solid substrata does not proceed according to expectations based on cell surface

  6. DMPD: Innate immune sensing of pathogens and danger signals by cell surface Toll-likereceptors. [Dynamic Macrophage Pathway CSML Database

    Lifescience Database Archive (English)

    Full Text Available 17275324 Innate immune sensing of pathogens and danger signals by cell surface Toll... Show Innate immune sensing of pathogens and danger signals by cell surface Toll-likereceptors. PubmedID 172...75324 Title Innate immune sensing of pathogens and danger signals by cell surface

  7. Evaluation of electrical energy consumption and reduction potential at the 7th Army Training Command (ATC), U. S. Army, Europe. Final report

    Energy Technology Data Exchange (ETDEWEB)

    Taylor, W.R.; Dubravec, M.A.

    1990-05-01

    The 7th Army Training Command (ATC), U.S. Army, Europe, has accrued increasingly higher electrical utility bills over the past few years. As is true at most installations, the 7th ATC buildings are not equipped with electricity meters so it is difficult to identify the major power consumers. To determine electrical energy use patterns and potentially find energy conservation opportunities (ECOs), the 7th ATC asked the U.S. Army Construction Engineering Research Laboratory (USACERL) to study electricity consumption at two of its communities, Grafenwohr and Hohenfels, Federal Republic of Germany. Results of the predictive modeling and field testing showed several possible ECOs at Grafenwohr and Hohenfels, such as dimming exterior lights during some hours of the night, providing better controls on oversized ventilation systems in gymnasiums, and training dining hall personnel in energy-conservative practices. A major electrical power consumer is the training ranges; however, since the training mission has priority over energy use, little can be done at this time to reduce consumption there. In the future, target ranges may become available that provide the same quality of training with a lower energy demand.

  8. Exploring action potential initiation in neurons exposed to DC electric fields through dynamical analysis of conductance-based model

    Science.gov (United States)

    Yi, Guo-Sheng; Wang, Jiang; Han, Chun-Xiao; Deng, Bin; Wei, Xi-Le; Jin, Qi-Tao

    2014-05-01

    Noninvasive direct current (DC) electric stimulation of central nervous system is today a promising therapeutic option to alleviate the symptoms of a number of neurological disorders. Despite widespread use of this noninvasive brain modulation technique, a generalizable explanation of its biophysical basis has not been described which seriously restricts its application and development. This paper investigated the dynamical behaviors of Hodgkin's three classes of neurons exposed to DC electric field based on a conductance-based neuron model. With phase plane and bifurcation analysis, the different responses of each class of neuron to the same stimulation are shown to derive from distinct spike initiating dynamics. Under the effects of negative DC electric field, class 1 neuron generates repetitive spike through a saddle-node on invariant circle (SNIC) bifurcation, while it ceases this repetitive behavior through a Hopf bifurcation; Class 2 neuron generates repetitive spike through a Hopf bifurcation, meanwhile it ceases this repetitive behavior also by a Hopf bifurcation; Class 3 neuron can generate single spike through a quasi-separatrix-crossing (QSC) at first, then it generates repetitive spike through a Hopf bifurcation, while it ceases this repetitive behavior through a SNIC bifurcation. Furthermore, three classes of neurons' spiking frequency f-electric field E (f-E) curves all have parabolic shape. Our results highlight the effects of external DC electric field on neuronal activity from the biophysical modeling point of view. It can contribute to the application and development of noninvasive DC brain modulation technique.

  9. Material properties and electrical stimulation regimens of polycaprolactone fumarate-polypyrrole scaffolds as potential conductive nerve conduits.

    Science.gov (United States)

    Moroder, Philipp; Runge, M Brett; Wang, Huan; Ruesink, Terry; Lu, Lichun; Spinner, Robert J; Windebank, Anthony J; Yaszemski, Michael J

    2011-03-01

    The mechanical and electrical properties of polycaprolactone fumarate-polypyrrole (PCLF-PPy) scaffolds were studied under physiological conditions to evaluate their ability to maintain the material properties necessary for application as conductive nerve conduits. PC12 cells cultured on PCLF-PPy scaffolds were stimulated with regimens of 10 μA of either a constant or a 20 Hz frequency current passed through the scaffolds for 1h per day. PC12 cellular morphologies were analyzed by fluorescence microscopy after 48 h. PCLF-PPy scaffolds exhibited excellent mechanical properties at 37 °C which would allow suturing and flexibility. The surface resistivity of the scaffolds was 2 kΩ and the scaffolds were electrically stable during the application of electrical stimulation (ES). In vitro studies showed significant increases in the percentage of neurite bearing cells, number of neurites per cell and neurite length in the presence of ES compared with no ES. Additionally, extending neurites were observed to align in the direction of the applied current. This study shows that electrically conductive PCLF-PPy scaffolds possess the material properties necessary for application as nerve conduits. Additionally, the capability to significantly enhance and direct neurite extension by passing an electrical current through PCLF-PPy scaffolds renders them even more promising as future therapeutic treatments for severe nerve injuries. Copyright © 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

  10. Material properties and electrical stimulation regimens through polycaprolactone fumarate-polypyrrole scaffolds as potential conductive nerve conduits

    Science.gov (United States)

    Moroder, Philipp; Wang, Huan; Ruesink, Terry; Lu, Lichun; Windebank, Anthony J.; Yaszemski, Michael J.; Runge, M. Brett

    2010-01-01

    Mechanical and electrical properties of polycaprolactone fumarate-polypyrrole (PCLF-PPy) scaffolds were studied under physiological conditions to evaluate their ability to maintain material properties necessary for application as conductive nerve conduits. PC12 cells cultured on PCLF-PPy scaffolds were stimulated with regimens of 10 μA of constant or 20 Hz frequency current passed through the scaffolds for 1 h/day. PC12 cellular morphologies were analyzed by fluorescence microscopy after 48 h. PCLF-PPy scaffolds exhibited excellent mechanical properties at 37°C which would allow suturing and flexibility. The surface resistivity of the scaffolds was 2kΩ and the scaffolds were electrically stable during application of electrical stimulation (ES). In vitro studies showed significant increases in percentage of neurite bearing cells, number of neurites per cell and neurite length in the presence of ES compared to no ES. Additionally, extending neurites were observed to align in the direction of the applied current. This study shows that electrically conductive PCLF-PPy scaffolds possess material properties necessary for application as nerve conduits. Additionally, the capability to significantly enhance and direct neurite extension by passing electrical current through PCLF-PPy scaffolds renders them even more promising as future therapeutic treatments for severe nerve injuries. PMID:20965280

  11. Structural changes in single membranes in response to an applied transmembrane electric potential revealed by time-resolved neutron/X-ray interferometry.

    Science.gov (United States)

    Tronin, A; Chen, C-H; Gupta, S; Worcester, D; Lauter, V; Strzalka, J; Kuzmenko, I; Blasie, J K

    2013-08-30

    The profile structure of a hybrid lipid bilayer, tethered to the surface of an inorganic substrate and fully hydrated with a bulk aqueous medium in an electrochemical cell, was investigated as a function of the applied transbilayer electric potential via time-resolved neutron reflectivity, enhanced by interferometry. Significant, and fully reversible structural changes were observed in the distal half (with respect to the substrate surface) of the hybrid bilayer comprised of a zwitterionic phospholipid in response to a +100mV potential with respect to 0mV. These arise presumably due to reorientation of the electric dipole present in the polar headgroup of the phospholipid and its resulting effect on the thickness of the phospholipid's hydrocarbon chain layer within the hybrid bilayer's profile structure. The profile structure of the voltage-sensor domain from a voltage-gated ion channel protein within a phospholipid bilayer membrane, tethered to the surface of an inorganic substrate and fully hydrated with a bulk aqueous medium in an electrochemical cell, was also investigated as a function of the applied transmembrane electric potential via time-resolved X-ray reflectivity, enhanced by interferometry. Significant, fully-reversible, and different structural changes in the protein were detected in response to ±100mV potentials with respect to 0mV. The approach employed is that typical of transient spectroscopy, shown here to be applicable to both neutron and X-ray reflectivity of thin films.

  12. Non-contact electric potential measurements of electrode components in an operating polymer electrolyte fuel cell by near ambient pressure XPS.

    Science.gov (United States)

    Yu, Liwei; Takagi, Yasumasa; Nakamura, Takahiro; Sekizawa, Oki; Sakata, Tomohiro; Uruga, Tomoya; Tada, Mizuki; Iwasawa, Yasuhiro; Samjeské, Gabor; Yokoyama, Toshihiko

    2017-11-22

    Photoelectron spectroscopy has the advantage of providing electric potentials by non-contact measurements based on the kinetic energy shift in component potential. We performed operando hard X-ray photoelectron spectroscopy (HAXPES) measurements with an 8 keV excitation source to measure the shift in electron kinetic energies as a function of the voltages of all the components at the anode and cathode electrodes of a polymer electrolyte fuel cell (PEFC). At the cathode electrode, when we increase the voltage between the cathode and anode from 0.2 to 1.2 V, the O 1s and F 1s peaks shift to a lower binding energy and the magnitude of the energy shift is equal to the voltage. The Pt 3d and C 1s peaks do not shift with the voltage since platinum nanoparticles and carbon supports at the cathode electrode have ground contact. In contrast to the cathode electrode, the peak shifts of all the components at the anode electrode show the same amount of shift as the voltages. It is clear that the change in the potential difference occurs only in an electrical double layer at the interface between the cathode electrode (Pt/C) and the electrolyte (Nafion and water), and that the anode electrode is in equilibrium as a pseudo-hydrogen electrode. Moreover, the electric potential variation of the cathode electrode in a PEFC under a power generation condition was also directly detected by operando HAXPES.

  13. Staphylococcus aureus Protein A Mediates Interspecies Interactions at the Cell Surface of Pseudomonas aeruginosa.

    Science.gov (United States)

    Armbruster, Catherine R; Wolter, Daniel J; Mishra, Meenu; Hayden, Hillary S; Radey, Matthew C; Merrihew, Gennifer; MacCoss, Michael J; Burns, Jane; Wozniak, Daniel J; Parsek, Matthew R; Hoffman, Lucas R

    2016-05-24

    While considerable research has focused on the properties of individual bacteria, relatively little is known about how microbial interspecies interactions alter bacterial behaviors and pathogenesis. Staphylococcus aureus frequently coinfects with other pathogens in a range of different infectious diseases. For example, coinfection by S. aureus with Pseudomonas aeruginosa occurs commonly in people with cystic fibrosis and is associated with higher lung disease morbidity and mortality. S. aureus secretes numerous exoproducts that are known to interact with host tissues, influencing inflammatory responses. The abundantly secreted S. aureus staphylococcal protein A (SpA) binds a range of human glycoproteins, immunoglobulins, and other molecules, with diverse effects on the host, including inhibition of phagocytosis of S. aureus cells. However, the potential effects of SpA and other S. aureus exoproducts on coinfecting bacteria have not been explored. Here, we show that S. aureus-secreted products, including SpA, significantly alter two behaviors associated with persistent infection. We found that SpA inhibited biofilm formation by specific P. aeruginosa clinical isolates, and it also inhibited phagocytosis by neutrophils of all isolates tested. Our results indicate that these effects were mediated by binding to at least two P. aeruginosa cell surface structures-type IV pili and the exopolysaccharide Psl-that confer attachment to surfaces and to other bacterial cells. Thus, we found that the role of a well-studied S. aureus exoproduct, SpA, extends well beyond interactions with the host immune system. Secreted SpA alters multiple persistence-associated behaviors of another common microbial community member, likely influencing cocolonization and coinfection with other microbes. Bacteria rarely exist in isolation, whether on human tissues or in the environment, and they frequently coinfect with other microbes. However, relatively little is known about how

  14. Surfactant-modified yeast whole-cell biocatalyst displaying lipase on cell surface for enzymatic production of structured lipids in organic media.

    Science.gov (United States)

    Hama, Shinji; Yoshida, Ayumi; Nakashima, Kazunori; Noda, Hideo; Fukuda, Hideki; Kondo, Akihiko

    2010-06-01

    The cell surface engineering system, in which functional proteins are genetically displayed on microbial cell surfaces, has recently become a powerful tool for applied biotechnology. Here, we report on the surfactant modification of surface-displayed lipase to improve its performance for enzymatic synthesis reactions. The lipase activities of the surfactant-modified yeast displaying Rhizopus oryzae lipase (ROL) were evaluated in both aqueous and nonaqueous systems. Despite the similar lipase activities of control and surfactant-modified cells in aqueous media, the treatment with nonionic surfactants increased the specific lipase activity of the ROL-displaying yeast in n-hexane. In particular, the Tween 20-modified cells increased the cell surface hydrophobicity significantly among a series of Tween surfactants tested, resulting in 8-30 times higher specific activity in organic solvents with relatively high log P values. The developed cells were successfully used for the enzymatic synthesis of phospholipids and fatty acid methyl esters in n-hexane, whereas the nontreated cells produced a significantly low yield. Our results thus indicate that surfactant modification of the cell surface can enhance the potential of the surface-displayed lipase for bioconversion.

  15. Phosphorylation of the type II transmembrane serine protease, TMPRSS13, in hepatocyte growth factor activator inhibitor-1 and -2-mediated cell-surface localization.

    Science.gov (United States)

    Murray, Andrew S; Varela, Fausto A; Hyland, Thomas E; Schoenbeck, Andrew J; White, Jordan M; Tanabe, Lauren M; Todi, Sokol V; List, Karin

    2017-09-08

    TMPRSS13 is a member of the type II transmembrane serine protease (TTSP) family. Although various TTSPs have been characterized in detail biochemically and functionally, the basic properties of TMPRSS13 remain unclear. Here, we investigate the activation, inhibition, post-translational modification, and localization of TMPRSS13. We show that TMPRSS13 is a glycosylated, active protease and that its own proteolytic activity mediates zymogen cleavage. Full-length, active TMPRSS13 exhibits impaired cell-surface expression in the absence of the cognate Kunitz-type serine protease inhibitors, hepatocyte growth factor activator inhibitor (HAI)-1 or HAI-2. Concomitant presence of TMPRSS13 with either HAI-1 or -2 mediates phosphorylation of residues in the intracellular domain of the protease, and it coincides with efficient transport of the protease to the cell surface and its subsequent shedding. Cell-surface labeling experiments indicate that the dominant form of TMPRSS13 on the cell surface is phosphorylated, whereas intracellular TMPRSS13 is predominantly non-phosphorylated. These data provide novel insight into the cellular properties of TMPRSS13 and highlight phosphorylation of TMPRSS13 as a novel post-translational modification of this TTSP family member and potentially other members of this family of proteases. © 2017 by The American Society for Biochemistry and Molecular Biology, Inc.

  16. Cell Surface Properties of Lactococcus lactis Reveal Milk Protein Binding Specifically Evolved in Dairy Isolates

    Directory of Open Access Journals (Sweden)

    Mariya Tarazanova

    2017-09-01

    Full Text Available Surface properties of bacteria are determined by the molecular composition of the cell wall and they are important for interactions of cells with their environment. Well-known examples of bacterial interactions with surfaces are biofilm formation and the fermentation of solid materials like food and feed. Lactococcus lactis is broadly used for the fermentation of cheese and buttermilk and it is primarily isolated from either plant material or the dairy environment. In this study, we characterized surface hydrophobicity, charge, emulsification properties, and the attachment to milk proteins of 55 L. lactis strains in stationary and exponential growth phases. The attachment to milk protein was assessed through a newly developed flow cytometry-based protocol. Besides finding a high degree of biodiversity, phenotype-genotype matching allowed the identification of candidate genes involved in the modification of the cell surface. Overexpression and gene deletion analysis allowed to verify the predictions for three identified proteins that altered surface hydrophobicity and attachment of milk proteins. The data also showed that lactococci isolated from a dairy environment bind higher amounts of milk proteins when compared to plant isolates. It remains to be determined whether the alteration of surface properties also has potential to alter starter culture functionalities.

  17. Enhancing the antitumor efficacy of a cell-surface death ligand by covalent membrane display

    Science.gov (United States)

    Nair, Pradeep M.; Flores, Heather; Gogineni, Alvin; Marsters, Scot; Lawrence, David A.; Kelley, Robert F.; Ngu, Hai; Sagolla, Meredith; Komuves, Laszlo; Bourgon, Richard; Settleman, Jeffrey; Ashkenazi, Avi

    2015-01-01

    TNF superfamily death ligands are expressed on the surface of immune cells and can trigger apoptosis in susceptible cancer cells by engaging cognate death receptors. A recombinant soluble protein comprising the ectodomain of Apo2 ligand/TNF-related apoptosis-inducing ligand (Apo2L/TRAIL) has shown remarkable preclinical anticancer activity but lacked broad efficacy in patients, possibly owing to insufficient exposure or potency. We observed that antibody cross-linking substantially enhanced cytotoxicity of soluble Apo2L/TRAIL against diverse cancer cell lines. Presentation of the ligand on glass-supported lipid bilayers enhanced its ability to drive receptor microclustering and apoptotic signaling. Furthermore, covalent surface attachment of Apo2L/TRAIL onto liposomes—synthetic lipid-bilayer nanospheres—similarly augmented activity. In vivo, liposome-displayed Apo2L/TRAIL achieved markedly better exposure and antitumor activity. Thus, covalent synthetic-membrane attachment of a cell-surface ligand enhances efficacy, increasing therapeutic potential. These findings have translational implications for liposomal approaches as well as for Apo2L/TRAIL and other clinically relevant TNF ligands. PMID:25902490

  18. Enhanced Growth Inhibition of Osteosarcoma by Cytotoxic Polymerized Liposomal Nanoparticles Targeting the Alcam Cell Surface Receptor

    Directory of Open Access Journals (Sweden)

    Noah Federman

    2012-01-01

    Full Text Available Osteosarcoma is the most common primary malignancy of bone in children, adolescents, and adults. Despite extensive surgery and adjuvant aggressive high-dose systemic chemotherapy with potentially severe bystander side effects, cure is attainable in about 70% of patients with localized disease and only 20%–30% of those patients with metastatic disease. Targeted therapies clearly are warranted in improving our treatment of this adolescent killer. However, a lack of osteosarcoma-associated/specific markers has hindered development of targeted therapeutics. We describe a novel osteosarcoma-associated cell surface antigen, ALCAM. We, then, create an engineered anti-ALCAM-hybrid polymerized liposomal nanoparticle immunoconjugate (α-AL-HPLN to specifically target osteosarcoma cells and deliver a cytotoxic chemotherapeutic agent, doxorubicin. We have demonstrated that α-AL-HPLNs have significantly enhanced cytotoxicity over untargeted HPLNs and over a conventional liposomal doxorubicin formulation. In this way, α-AL-HPLNs are a promising new strategy to specifically deliver cytotoxic agents in osteosarcoma.

  19. Bioadsorption of Rare Earth Elements through Cell Surface Display of Lanthanide Binding Tags.

    Science.gov (United States)

    Park, Dan M; Reed, David W; Yung, Mimi C; Eslamimanesh, Ali; Lencka, Malgorzata M; Anderko, Andrzej; Fujita, Yoshiko; Riman, Richard E; Navrotsky, Alexandra; Jiao, Yongqin

    2016-03-01

    With the increasing demand for rare earth elements (REEs) in many emerging clean energy technologies, there is an urgent need for the development of new approaches for efficient REE extraction and recovery. As a step toward this goal, we genetically engineered the aerobic bacterium Caulobacter crescentus for REE adsorption through high-density cell surface display of lanthanide binding tags (LBTs) on its S-layer. The LBT-displayed strains exhibited enhanced adsorption of REEs compared to cells lacking LBT, high specificity for REEs, and an adsorption preference for REEs with small atomic radii. Adsorbed Tb(3+) could be effectively recovered using citrate, consistent with thermodynamic speciation calculations that predicted strong complexation of Tb(3+) by citrate. No reduction in Tb(3+) adsorption capacity was observed following citrate elution, enabling consecutive adsorption/desorption cycles. The LBT-displayed strain was effective for extracting REEs from the acid leachate of core samples collected at a prospective rare earth mine. Our collective results demonstrate a rapid, efficient, and reversible process for REE adsorption with potential industrial application for REE enrichment and separation.

  20. Immunochemical detection of prion protein on dipsticks prepared with crystalline bacterial cell-surface layers.

    Science.gov (United States)

    Völkel, Dirk; Zimmermann, Klaus; Breitwieser, Andreas; Pable, Sabrina; Glatzel, Markus; Scheiflinger, Friedrich; Schwarz, Hans P; Sara, Margit; Sleytr, Uwe B; Dorner, Friedrich

    2003-12-01

    Transmissible spongiform encephalopathy (TSE) represents a spectrum of diseases affecting humans and animals. A definitive diagnosis of TSEs is only possible by postmortem identification of pathologic prion protein in brain tissue that has been treated with protease. The pathologic protein is detected by Western blot analysis or ELISA methods. The bovine spongiform encephalopathy crisis and occurrence of a new variant of CJD has increased demand for rapid and simple assays. A dipstick assay has been developed for prion diagnosis based on a sandwich ELISA specific for prion protein, and crystalline bacterial cell-surface layers (S-layers) were used as an immobilization matrix. The usefulness of the dipstick assay was evaluated by determining the detection limit, comparison with other methods, and analysis of CJD samples. The sensitivity of the prion dipsticks was similar to that published for time-resolved fluorescence ELISA methods. After protease treatment, pathologic prion protein could be detected specifically. The dipstick assay is a sensitive and specific test useful for the detection of prion protein. The simplicity of the S-layer dipstick lends itself to a variety of potential applications including field diagnostics.