WorldWideScience

Sample records for cell product design

  1. MOLTEN CARBONATE FUEL CELL PRODUCT DESIGN IMPROVEMENT

    Energy Technology Data Exchange (ETDEWEB)

    H.C. Maru; M. Farooque

    2003-03-01

    The program efforts are focused on technology and system optimization for cost reduction, commercial design development, and prototype system field trials. The program is designed to advance the carbonate fuel cell technology from full-size field test to the commercial design. FuelCell Energy, Inc. (FCE) is in the later stage of the multiyear program for development and verification of carbonate fuel cell based power plants supported by DOE/NETL with additional funding from DOD/DARPA and the FuelCell Energy team. FCE has scaled up the technology to full-size and developed DFC{reg_sign} stack and balance-of-plant (BOP) equipment technology to meet product requirements, and acquired high rate manufacturing capabilities to reduce cost. FCE has designed submegawatt (DFC300A) and megawatt (DFC1500 and DFC3000) class fuel cell products for commercialization of its DFC{reg_sign} technology. A significant progress was made during the reporting period. The reforming unit design was optimized using a three-dimensional stack simulation model. Thermal and flow uniformities of the oxidant-In flow in the stack module were improved using computational fluid dynamics based flow simulation model. The manufacturing capacity was increased. The submegawatt stack module overall cost was reduced by {approx}30% on a per kW basis. An integrated deoxidizer-prereformer design was tested successfully at submegawatt scale using fuels simulating digester gas, coal bed methane gas and peak shave (natural) gas.

  2. Direct fuel cell product design improvement

    Energy Technology Data Exchange (ETDEWEB)

    Maru, H.C.; Farooque, M. [Energy Research Corp., Danbury, CT (United States)

    1996-12-31

    Significant milestones have been attained towards the technology development field testing and commercialization of direct fuel cell power plant since the 1994 Fuel Cell Seminar. Under a 5-year cooperative agreement with the Department of Energy signed in December 1994, Energy Research Corporation (ERC) has been developing the design for a MW-scale direct fuel cell power plant with input from previous technology efforts and the Santa Clara Demonstration Project. The effort encompasses product definition in consultation with the Fuel Cell Commercialization Group, potential customers, as well as extensive system design and packaging. Manufacturing process improvements, test facility construction, cell component scale up, performance and endurance improvements, stack engineering, and critical balance-of-plant development are also addressed. Major emphasis of this product design improvement project is on increased efficiency, compactness and cost reduction to establish a competitive place in the market. A 2.85 MW power plant with an efficiency of 58% and a footprint of 420 m{sup 2} has been designed. Component and subsystem testing is being conducted at various levels. Planning and preparation for verification of a full size prototype unit are in progress. This paper presents the results obtained since the last fuel cell seminar.

  3. Molten carbonate fuel cell product design improvement

    Energy Technology Data Exchange (ETDEWEB)

    P. Voyentzie; T. Leo; A. Kush; L. Christner; G. Carlson; C. Yuh

    1998-12-20

    Drawing on the manufacture, field test, and post-test experience of the sixteen Santa Clara Demonstration Project (SCDP) stacks, ERC is finalizing the next generation commercial entry product design. The second generation cells are 50% larger in area, 40% lighter on equal geometric area basis, and 30% thinner than the earlier design. These improvements have resulted in doubling of the full-height stack power. A low-cost and high-strength matrix has also been developed for improving product ruggedness. The low-cost advanced cell design incorporating these improvements has been refined through six short stack tests. Power production per cell of two times the SCDP maximum power operation, over ten thermal cycles, and overall operating flexibility with respect to load and thermal changes have been demonstrated in these short stack tests. An internally insulated stack enclosure has been designed and fabricated to eliminate the need for an inert gas environment during operation. ERC has acquired the capability for testing 400kW full-height direct fuel ceil (DFC) stack and balance-of-plant equipment. With the readiness of the power plant test facility, the cell package design, and the stack module, full-height stack testing has begun. The first full- height stack incorporating the post-SCDP second generation design was completed. The stack reached a power level of 253 kW, setting a world record for the highest power production from the advanced fuel cell system. Excellent performance uniformity at this power level affirmed manufacturing reproducibility of the components at the factory. This unoptimized small size test has achieved pipeline natural gas to DC electricity conversion efficiency of 47% (based on lower heating value - LHV) including the parasitic power consumed by the BOP equipment; that should translate to more than 50% efficiency in commercial operation, before employing cogeneration. The power plant system also operated smoothly. With the success of this

  4. MOLTEN CARBONATE FUEL CELL PRODUCT DESIGN IMPROVEMENT

    Energy Technology Data Exchange (ETDEWEB)

    H.C. Maru; M. Farooque

    2005-03-01

    The program was designed to advance the carbonate fuel cell technology from full-size proof-of-concept field test to the commercial design. DOE has been funding Direct FuelCell{reg_sign} (DFC{reg_sign}) development at FuelCell Energy, Inc. (FCE, formerly Energy Research Corporation) from an early state of development for stationary power plant applications. The current program efforts were focused on technology and system development, and cost reduction, leading to commercial design development and prototype system field trials. FCE, in Danbury, CT, is a world-recognized leader for the development and commercialization of high efficiency fuel cells that can generate clean electricity at power stations, or at distributed locations near the customers such as hospitals, schools, universities, hotels and other commercial and industrial applications. FCE has designed three different fuel cell power plant models (DFC300A, DFC1500 and DFC3000). FCE's power plants are based on its patented DFC{reg_sign} technology, where a hydrocarbon fuel is directly fed to the fuel cell and hydrogen is generated internally. These power plants offer significant advantages compared to the existing power generation technologies--higher fuel efficiency, significantly lower emissions, quieter operation, flexible siting and permitting requirements, scalability and potentially lower operating costs. Also, the exhaust heat by-product can be used for cogeneration applications such as high-pressure steam, district heating and air conditioning. Several sub-MW power plants based on the DFC design are currently operating in Europe, Japan and the US. Several one-megawatt power plant design was verified by operation on natural gas at FCE. This plant is currently installed at a customer site in King County, WA under another US government program and is currently in operation. Because hydrogen is generated directly within the fuel cell module from readily available fuels such as natural gas and

  5. MOLTEN CARBONATE FUEL CELL PRODUCT DESIGN IMPROVEMENT

    Energy Technology Data Exchange (ETDEWEB)

    Unknown

    2000-01-01

    The FCE PDI program is designed to advance the carbonate fuel cell technology from the current full-size field test to the commercial design. The specific objectives selected to attain the overall program goal are: Define power plant requirements and specifications; Establish the design for a multifuel, low-cost, modular, market-responsive power plant; Resolve power plant manufacturing issues and define the design for the commercial-scale manufacturing facility; Define the stack and balance-of-plant (BOP) equipment packaging arrangement, and module designs; Acquire capability to support developmental testing of stacks and critical BOP equipment to prepare for commercial design; and Resolve stack and BOP equipment technology issues, and design, build and field test a modular prototype power plant to demonstrate readiness for commercial entry.

  6. Engineering microbial cell factories for the production of plant natural products: from design principles to industrial-scale production.

    Science.gov (United States)

    Liu, Xiaonan; Ding, Wentao; Jiang, Huifeng

    2017-07-19

    Plant natural products (PNPs) are widely used as pharmaceuticals, nutraceuticals, seasonings, pigments, etc., with a huge commercial value on the global market. However, most of these PNPs are still being extracted from plants. A resource-conserving and environment-friendly synthesis route for PNPs that utilizes microbial cell factories has attracted increasing attention since the 1940s. However, at the present only a handful of PNPs are being produced by microbial cell factories at an industrial scale, and there are still many challenges in their large-scale application. One of the challenges is that most biosynthetic pathways of PNPs are still unknown, which largely limits the number of candidate PNPs for heterologous microbial production. Another challenge is that the metabolic fluxes toward the target products in microbial hosts are often hindered by poor precursor supply, low catalytic activity of enzymes and obstructed product transport. Consequently, despite intensive studies on the metabolic engineering of microbial hosts, the fermentation costs of most heterologously produced PNPs are still too high for industrial-scale production. In this paper, we review several aspects of PNP production in microbial cell factories, including important design principles and recent progress in pathway mining and metabolic engineering. In addition, implemented cases of industrial-scale production of PNPs in microbial cell factories are also highlighted.

  7. Conceptual design report of hot cell modification and process for fission Mo-99 production

    Energy Technology Data Exchange (ETDEWEB)

    Park, Jin Ho; Choung, W. M.; Lee, K. I.; Hwang, D. S.; Kim, Y. K.; Park, K. B.; Jung, Y. J.; Kim, D. S.; Park, Y. C

    2001-05-01

    In this conceptual design report, the basic data and design guides for detail design of fission Mo-99 production process and hot cell modification are included.The basic data and design guides for detail design of fission Mo-99 production process contains following contents. -design capacity, the basic process, process flow diagram, process material balance, process data. The basic data and design guides for modification of existing hot cell contains following contents. - plot plan of hot cell facility, the plan for shield reinforcement of hot cell, the plan for management and storage of high level liquid wastes, the plan of ventilation system, the plan for modification of auxiliary facilities. And also, the results of preliminary safety analysis(normal operation and accidents) and criticality analysis are included in this conceptual design report.

  8. Conceptual design report of hot cell modification and process for fission Mo-99 production

    International Nuclear Information System (INIS)

    Park, Jin Ho; Choung, W. M.; Lee, K. I.; Hwang, D. S.; Kim, Y. K.; Park, K. B.; Jung, Y. J.; Kim, D. S.; Park, Y. C.

    2001-05-01

    In this conceptual design report, the basic data and design guides for detail design of fission Mo-99 production process and hot cell modification are included.The basic data and design guides for detail design of fission Mo-99 production process contains following contents. -design capacity, the basic process, process flow diagram, process material balance, process data. The basic data and design guides for modification of existing hot cell contains following contents. - plot plan of hot cell facility, the plan for shield reinforcement of hot cell, the plan for management and storage of high level liquid wastes, the plan of ventilation system, the plan for modification of auxiliary facilities. And also, the results of preliminary safety analysis(normal operation and accidents) and criticality analysis are included in this conceptual design report

  9. Production and Reliability Oriented SOFC Cell and Stack Design

    DEFF Research Database (Denmark)

    Hauth, Martin; Lawlor, Vincent; Cartellieri, Peter

    2017-01-01

    established. The probabilistic models were related to the experimentally obtained properties of base materials to establish a statistical relationship between the material properties and the most relevant load effects. Software algorithms for meta models that allow the detection of relationships between input...... in production, material and operating parameters for the optimization phase. A methodology for 3D description of spatial distribution of material properties based on a random field models was developed and validated by experiments. Homogenized material models on multiple levels of the SOFC stack were...

  10. Statistical experimental designs for the production of secondary metabolites in plant cell suspension cultures.

    Science.gov (United States)

    Schmitz, Christian; Fritsch, Leonie; Fischer, Rainer; Schillberg, Stefan; Rasche, Stefan

    2016-12-01

    Statistical experimental designs, also known as the "design of experiments" (DoE) approach, are widely used to improve not only technical processes but also to answer questions in the agricultural, medical and social sciences. Although many articles have been published about the application of DoE in these fields, few studies have addressed the use of DoE in the plant sciences, particularly in the context of plant cell suspension cultures (PCSCs). Compounds derived from PCSCs can be developed as pharmaceuticals, chemical feedstocks and cosmetic ingredients, and statistical experimental designs can be used to improve the productivity of the cells and the yield and/or quality of the target compounds in a cost efficient manner. In this article, we summarize recent findings concerning the application of statistical approaches to improve the performance of PCSCs and discuss the potential future applications of this approach.

  11. The Role of Synthetic Biology in the Design of Microbial Cell Factories for Biofuel Production

    Science.gov (United States)

    Colin, Verónica Leticia; Rodríguez, Analía; Cristóbal, Héctor Antonio

    2011-01-01

    Insecurity in the supply of fossil fuels, volatile fuel prices, and major concerns regarding climate change have sparked renewed interest in the production of fuels from renewable resources. Because of this, the use of biodiesel has grown dramatically during the last few years and is expected to increase even further in the future. Biodiesel production through the use of microbial systems has marked a turning point in the field of biofuels since it is emerging as an attractive alternative to conventional technology. Recent progress in synthetic biology has accelerated the ability to analyze, construct, and/or redesign microbial metabolic pathways with unprecedented precision, in order to permit biofuel production that is amenable to industrial applications. The review presented here focuses specifically on the role of synthetic biology in the design of microbial cell factories for efficient production of biodiesel. PMID:22028591

  12. The Role of Synthetic Biology in the Design of Microbial Cell Factories for Biofuel Production

    Directory of Open Access Journals (Sweden)

    Verónica Leticia Colin

    2011-01-01

    Full Text Available Insecurity in the supply of fossil fuels, volatile fuel prices, and major concerns regarding climate change have sparked renewed interest in the production of fuels from renewable resources. Because of this, the use of biodiesel has grown dramatically during the last few years and is expected to increase even further in the future. Biodiesel production through the use of microbial systems has marked a turning point in the field of biofuels since it is emerging as an attractive alternative to conventional technology. Recent progress in synthetic biology has accelerated the ability to analyze, construct, and/or redesign microbial metabolic pathways with unprecedented precision, in order to permit biofuel production that is amenable to industrial applications. The review presented here focuses specifically on the role of synthetic biology in the design of microbial cell factories for efficient production of biodiesel.

  13. Design, Engineering, and Construction of Photosynthetic Microbial Cell Factories for Renewable Solar Fuel Production

    Energy Technology Data Exchange (ETDEWEB)

    Lindblad, Peter; Lindberg, Pia; Stensjoe, Karin (Photochemistry and Molecular Science, Dept. of Chemistry-Aangstroem Laboratory, Uppsala Univ., Uppsala (Sweden)), E-mail: Peter.Lindblad@kemi.uu.se; Oliveira, Paulo (Instituto de Biologia Molecular e Celular, Porto (Portugal)); Heidorn, Thorsten (Bioforsk-Norwegian Inst. for Agricultural and Environmental Research, Aas Oslo, (Norway))

    2012-03-15

    There is an urgent need to develop sustainable solutions to convert solar energy into energy carriers used in the society. In addition to solar cells generating electricity, there are several options to generate solar fuels. This paper outlines and discusses the design and engineering of photosynthetic microbial systems for the generation of renewable solar fuels, with a focus on cyanobacteria. Cyanobacteria are prokaryotic microorganisms with the same type of photosynthesis as higher plants. Native and engineered cyanobacteria have been used by us and others as model systems to examine, demonstrate, and develop photobiological H{sub 2} production. More recently, the production of carbon-containing solar fuels like ethanol, butanol, and isoprene have been demonstrated. We are using a synthetic biology approach to develop efficient photosynthetic microbial cell factories for direct generation of biofuels from solar energy. Present progress and advances in the design, engineering, and construction of such cyanobacterial cells for the generation of a portfolio of solar fuels, e.g., hydrogen, alcohols, and isoprene, are presented and discussed. Possibilities and challenges when introducing and using synthetic biology are highlighted

  14. Molted carbonate fuel cell product design and improvement - 4th quarter, 1995. Quarterly report, October 1, 1995--December 31, 1995

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1998-04-01

    The primary objective of this project is to establish the commercial readiness of MW-class IMHEX Molten Carbonate Fuel Cell power plants. Progress is described on marketing, systems design and analysis, product options and manufacturing.

  15. Ergonomic Product Design 21

    International Nuclear Information System (INIS)

    Kwon, Yeong Guk

    1996-09-01

    This book explains basic of ergonomic product design with human engineering, image engineering and strategy of that design, ergonomic industrial design, which includes product design to access the human engineering in development of new product and customer satisfaction, application technology of image engineering, industrial design of human engineering item and strategy of human engineering, a good ergonomic design. It also tells of examples of convenient design for human such as hardware product and software product in automobile, telephones for ergonomic product in the future, new goods and new technology, ergonomic product in house and office, and computers and robots in the future.

  16. Ergonomic Product Design 21

    Energy Technology Data Exchange (ETDEWEB)

    Kwon, Yeong Guk

    1996-09-15

    This book explains basic of ergonomic product design with human engineering, image engineering and strategy of that design, ergonomic industrial design, which includes product design to access the human engineering in development of new product and customer satisfaction, application technology of image engineering, industrial design of human engineering item and strategy of human engineering, a good ergonomic design. It also tells of examples of convenient design for human such as hardware product and software product in automobile, telephones for ergonomic product in the future, new goods and new technology, ergonomic product in house and office, and computers and robots in the future.

  17. A comprehensive review of microbial electrolysis cells (MEC reactor designs and configurations for sustainable hydrogen gas production

    Directory of Open Access Journals (Sweden)

    Abudukeremu Kadier

    2016-03-01

    Full Text Available Hydrogen gas has tremendous potential as an environmentally acceptable energy carrier for vehicles. A cutting edge technology called a microbial electrolysis cell (MEC can achieve sustainable and clean hydrogen production from a wide range of renewable biomass and wastewaters. Enhancing the hydrogen production rate and lowering the energy input are the main challenges of MEC technology. MEC reactor design is one of the crucial factors which directly influence on hydrogen and current production rate in MECs. The rector design is also a key factor to up-scaling. Traditional MEC designs incorporated membranes, but it was recently shown that membrane-free designs can lead to both high hydrogen recoveries and production rates. Since then multiple studies have developed reactors that operate without membranes. This review provides a brief overview of recent advances in research on scalable MEC reactor design and configurations.

  18. Models and methods for design and implementation of computer based control and monitoring systems for production cells

    DEFF Research Database (Denmark)

    Lynggaard, Hans Jørgen Birk

    based control and monitoring systems for production cells.The manufacturing environment and the current practice for engineering of cells control systems are described and automation software enablers are discussed. A number of problems related to these issues are identified. In order to support......This dissertation is concerned with the engineering, i.e. the designing and making, of industrial cell control systems. The focus is on automated robot welding cells in the shipbuilding industry. The industrial research project defines models and methods for design and implementation of computer...... engineering of cell control systems by the use of enablers, a generic cell control data model and an architecture are defined. Further, an engineering methodology is defined. The three element enablers, architecture and methodology consitutes the Cell Control Engineering concept which is defined and evaluated...

  19. HW/SW Design Space Exploration on the Production Cell Setup

    NARCIS (Netherlands)

    Groothuis, M.A.; Welch, P.H.; Roebbers, H.; Broenink, Johannes F.; Barnes, F.R.M.

    2009-01-01

    This paper describes and compares five CSP based and two CSP related process-oriented motion control system implementations that are made for our Production Cell demonstration setup. Five implementations are software-based and two are FPGA hardware-based. All implementations were originally made

  20. Product line design

    Czech Academy of Sciences Publication Activity Database

    Anderson, S. P.; Celik, Levent

    2015-01-01

    Roč. 157, May (2015), s. 517-526 ISSN 0022-0531 Institutional support: RVO:67985998 Keywords : product line design * product differentiation * second-degree price discrimination Subject RIV: AH - Economics Impact factor: 1.097, year: 2015

  1. Performance-enhancing drugs: design and production of redirected chimeric antigen receptor (CAR) T cells.

    Science.gov (United States)

    Levine, B L

    2015-03-01

    Performance enhancement of the immune system can now be generated through ex vivo gene modification of T cells in order to redirect native specificity to target tumor antigens. This approach combines the specificity of antibody therapy, the expanded response of cellular therapy and the memory activity of vaccine therapy. Recent clinical trials of chimeric antigen receptor (CAR) T cells directed toward CD19 as a stand-alone therapy have shown sustained complete responses in patients with acute lymphoblastic leukemia and chronic lymphocytic leukemia. As these drug products are individually derived from a patient's own cells, a different manufacturing approach is required for this kind of personalized therapy compared with conventional drugs. Key steps in the CAR T-cell manufacturing process include the selection and activation of isolated T cells, transduction of T cells to express CARs, ex vivo expansion of modified T cells and cryopreservation in infusible media. In this review, the steps involved in isolating, genetically modifying and scaling-out the CAR T cells for use in a clinical setting are described in the context of in-process and release testing and regulatory standards.

  2. Improving designer productivity

    Science.gov (United States)

    Hill, Gary C.

    1992-01-01

    Designer and design team productivity improves with skill, experience, and the tools available. The design process involves numerous trials and errors, analyses, refinements, and addition of details. Computerized tools have greatly speeded the analysis, and now new theories and methods, emerging under the label Artificial Intelligence (AI), are being used to automate skill and experience. These tools improve designer productivity by capturing experience, emulating recognized skillful designers, and making the essence of complex programs easier to grasp. This paper outlines the aircraft design process in today's technology and business climate, presenting some of the challenges ahead and some of the promising AI methods for meeting those challenges.

  3. Application of a quality by design approach to the cell culture process of monoclonal antibody production, resulting in the establishment of a design space.

    Science.gov (United States)

    Nagashima, Hiroaki; Watari, Akiko; Shinoda, Yasuharu; Okamoto, Hiroshi; Takuma, Shinya

    2013-12-01

    This case study describes the application of Quality by Design elements to the process of culturing Chinese hamster ovary cells in the production of a monoclonal antibody. All steps in the cell culture process and all process parameters in each step were identified by using a cause-and-effect diagram. Prospective risk assessment using failure mode and effects analysis identified the following four potential critical process parameters in the production culture step: initial viable cell density, culture duration, pH, and temperature. These parameters and lot-to-lot variability in raw material were then evaluated by process characterization utilizing a design of experiments approach consisting of a face-centered central composite design integrated with a full factorial design. Process characterization was conducted using a scaled down model that had been qualified by comparison with large-scale production data. Multivariate regression analysis was used to establish statistical prediction models for performance indicators and quality attributes; with these, we constructed contour plots and conducted Monte Carlo simulation to clarify the design space. The statistical analyses, especially for raw materials, identified set point values, which were most robust with respect to the lot-to-lot variability of raw materials while keeping the product quality within the acceptance criteria. © 2013 Wiley Periodicals, Inc. and the American Pharmacists Association.

  4. DESIGN VERSUS PRODUCT DEVELOPMENT?

    Directory of Open Access Journals (Sweden)

    Edu Grieco Mazzini Junior

    2015-03-01

    Full Text Available Design or product development? The questioning in relation to the employment of such terminology dates back to possible misconceptions regarding interpretation and approach them. Therefore, it can be stated that there are differences between these two terms? In this context, we highlight two views concerning the origin of the design. The first character creative and based on pre-industrial revolution, based on projective and methodologies directed towards the practice of designing products. And second, where design is understood as an industrial process: the development of new products includes, besides a creative procedure are developed, and symbolic formal solutions for products, but also a technical procedure based on the definition of the requirements concerning the product engineering. Is this the most widely accepted interpretation? theoretical studies were conducted from interpretations of the concept of the design proposed by the International Council of Societies of Industrial Design – ICSDI (2012, and for the characterization of the process of product development, we sought to develop an analogy between these two terminologies and can be proved that the use of one of terminology will not cause deviations of interpretation, since both deal with the life cycle of the product. The main results obtained until now the definition of design proposed by the ICSID where it is characterized as a creative activity focused on the complete life cycle of products is the more approaches of the concept of product development.

  5. Improving pension product design

    DEFF Research Database (Denmark)

    Konicz, Agnieszka Karolina; Mulvey, John M.

    2013-01-01

    Pension products characterized by linking an individual's savings directly to market returns represent the most popular, growing pension domain globally. These products are widely sold in contribution-defined pension schemes, labor market pensions, and individual schemes. However, available...... of an optimal pension product design, we present the operations research methods, which have potential to stimulate new thinking and add to actuarial practice....

  6. Chemical Product Design

    DEFF Research Database (Denmark)

    Gani, Rafiqul

    2004-01-01

    This paper highlights for a class of chemical products, the design process, their design with respect to the important issues, the need for appropriate tools and finally, lists some of the challenges and opportunities for the process systems engineering (PSE)/computer-aided process engineering...... (CAPE) community. The chemical products considered belong to the following types: chemical/biochemical/agrochemical products, coatings and solvents, food (nutraceuticals), HIM (household, industrial and institutional), personal care, pharmaceuticals and drugs. The challenges and opportunities...... are highlighted in terms of the needs for multi-level modeling with emphasis on property models that are suitable for computer-aided applications, flexible solution strategies that are able to solve a large range of chemical product design problems and finally, a systems chemical product design framework...

  7. Product line design

    Czech Academy of Sciences Publication Activity Database

    Anderson, S. P.; Celik, Levent

    2015-01-01

    Roč. 157, May (2015), s. 517-526 ISSN 0022-0531 R&D Projects: GA ČR(CZ) GA15-22540S Institutional support: PRVOUK-P23 Keywords : product line design * product differentiation * second-degree price discrimination Subject RIV: AH - Economics Impact factor: 1.097, year: 2015

  8. Product design and development engineering

    International Nuclear Information System (INIS)

    Lee, Kookhwan

    2008-01-01

    This book gives design of molded plastics, design of press product, design of die casting products, the application of communication terminal design, application and design of machine elements(screw, spring, bearing, gear, retaining ridge, drawing standards, KS and JIS material marks list), 3D CAD, concurrent engineering of product design, creative concept design.

  9. Computer aided product design

    DEFF Research Database (Denmark)

    Constantinou, Leonidas; Bagherpour, Khosrow; Gani, Rafiqul

    1996-01-01

    A general methodology for Computer Aided Product Design (CAPD) with specified property constraints which is capable of solving a large range of problems is presented. The methodology employs the group contribution approach, generates acyclic, cyclic and aromatic compounds of various degrees......-liquid equilibria (LLE), solid-liquid equilibria (SLE) and gas solubility. Finally, a computer program based on the extended methodology has been developed and the results from five case studies highlighting various features of the methodology are presented....

  10. Parallel experimental design and multivariate analysis provides efficient screening of cell culture media supplements to improve biosimilar product quality.

    Science.gov (United States)

    Brühlmann, David; Sokolov, Michael; Butté, Alessandro; Sauer, Markus; Hemberger, Jürgen; Souquet, Jonathan; Broly, Hervé; Jordan, Martin

    2017-07-01

    Rational and high-throughput optimization of mammalian cell culture media has a great potential to modulate recombinant protein product quality. We present a process design method based on parallel design-of-experiment (DoE) of CHO fed-batch cultures in 96-deepwell plates to modulate monoclonal antibody (mAb) glycosylation using medium supplements. To reduce the risk of losing valuable information in an intricate joint screening, 17 compounds were separated into five different groups, considering their mode of biological action. The concentration ranges of the medium supplements were defined according to information encountered in the literature and in-house experience. The screening experiments produced wide glycosylation pattern ranges. Multivariate analysis including principal component analysis and decision trees was used to select the best performing glycosylation modulators. Subsequent D-optimal quadratic design with four factors (three promising compounds and temperature shift) in shake tubes confirmed the outcome of the selection process and provided a solid basis for sequential process development at a larger scale. The glycosylation profile with respect to the specifications for biosimilarity was greatly improved in shake tube experiments: 75% of the conditions were equally close or closer to the specifications for biosimilarity than the best 25% in 96-deepwell plates. Biotechnol. Bioeng. 2017;114: 1448-1458. © 2017 Wiley Periodicals, Inc. © 2017 Wiley Periodicals, Inc.

  11. Designing Valuable Products with Design Sprint

    OpenAIRE

    Sari, Eunice; Tedjasaputra, Adi

    2017-01-01

    Part 6: Courses; International audience; The shift of creating valuable products, from only aesthetically pleasing, usable, or loveable products, has required adjustment of skills to create value as well as a mindset change. Instead of spending weeks and months to design a product, a rapid validation process takes place to explore how a product should be transformed into a valuable one. This course will teach the participants several key Design Sprint techniques in a nutshell (80 min). Google...

  12. Designing Product Families

    DEFF Research Database (Denmark)

    Pedersen, Per Erik Elgård; Miller, Thomas Dedenroth

    1998-01-01

    Manufacturing companies that operate with products aimed at different market segments and applications are experiencing an increasing demand from customers who expect products to be tailored to their exact needs rather than accepting general products based on average customer needs. This trend has...... led to a new business paradigm, "mass customization", where companies strive to provide highly customized products while still maintaining the efficiency of the classical mass production enterprise. One of the key factors in mass customization has been efficient use of product platforms...... as a foundation for the customization process, whereby the customized products become variants of a product family with a high degree of reuse and utilization of kinship between the individual variants.With this paper, we will discuss the development of platform based product families from three points of view...

  13. Improving pension product design

    DEFF Research Database (Denmark)

    Konicz, Agnieszka Karolina; Mulvey, John M.

    The paper develops a pension product that can be oered in a dened contribution pension scheme as a deferred or immediate life annuity. The product is characterized by linking an individual's savings directly to market returns and differs from the products available in the market by being adjusted......, expected lifetime salary progression, mandatory and voluntary pension contributions, expected state retirement pension, choice of assets, subjective lifetime expectancy and bequest motive. Specically, the asset allocation strategy, the payout prole and the level of insured sum dening the new product...

  14. Design and construction of a 7,500 liter immobilized cell reactor-separator for ethanol production from whey

    Energy Technology Data Exchange (ETDEWEB)

    Dale, M.C.

    1992-12-31

    A 7,500 liter reactor/separator has been constructed for the production of ethanol from concentrated whey permeate. This unit is sited in Hopkinton IA, across the street from a whey generating cheese plant A two phase construction project consisting of (1) building and testing a reactor/separator with a solvent absorber in a single unified housing, and (2) building and testing an extractive distillation/product stripper for the recovery of anhydrous ethanol is under way. The design capacity of this unit is 250,000 gal/yr of anhydrous product. Design and construction details of the reactor/absorber separator are given, and design parameters for the extractive distillation system are described.

  15. Micro Products - Product Development and Design

    DEFF Research Database (Denmark)

    Hansen, Hans Nørgaard

    2003-01-01

    -disciplinary competences. One example is sensors for chemical analysis of fluids, where chemistry, biology and flow mechanics all influence the design of the product and thereby the industrial fabrication of the product [1]. On the technological side the development has moved very fast, primarily driven by the need...... of the electronics industry to create still smaller chips with still larger capacity. Therefore the manufacturing technologies connected with micro/nano products in silicon are relatively highly developed compared to the technologies used for manufacturing micro products in metals, polymers and ceramics. For all...... technologies, however, it is a continuously increasing challenge to create the operational basis for an industrial production of micro products. As the products through product development processes are made applicable to a large number of customers, the pressure in regard to developing production technologies...

  16. Culture-Orientated Product Design

    Science.gov (United States)

    Moalosi, Richie; Popovic, Vesna; Hickling-Hudson, Anne

    2010-01-01

    There is little in-depth research that can assist designers to use culture as a catalyst for designing innovative products within Botswana's context. The concept of culture and design are intertwined, thus modifications stemming from cultural evolution both reflect and determine developments in design. The paper discusses an experimental design…

  17. Improving designer productivity. [artificial intelligence

    Science.gov (United States)

    Hill, Gary C.

    1992-01-01

    Designer and design team productivity improves with skill, experience, and the tools available. The design process involves numerous trials and errors, analyses, refinements, and addition of details. Computerized tools have greatly speeded the analysis, and now new theories and methods, emerging under the label Artificial Intelligence (AI), are being used to automate skill and experience. These tools improve designer productivity by capturing experience, emulating recognized skillful designers, and making the essence of complex programs easier to grasp. This paper outlines the aircraft design process in today's technology and business climate, presenting some of the challenges ahead and some of the promising AI methods for meeting these challenges.

  18. Use of a Plackett-Burman statistical design to determine the effect of selected amino acids on monoclonal antibody production in CHO cells.

    Science.gov (United States)

    González-Leal, I J; Carrillo-Cocom, L M; Ramírez-Medrano, A; López-Pacheco, F; Bulnes-Abundis, D; Webb-Vargas, Y; Alvarez, M M

    2011-01-01

    Culture media design is central to the optimization of monoclonal antibody (mAb) production. Although general strategies do not currently exist for optimization of culture media, the combined use of statistical design and analysis of experiments and strategies based on simple material balances can facilitate culture media design. In this study, we evaluate the effect of selected amino acids on the growth rate and monoclonal antibody production of a Chinese hamster ovary DG-44 (CHO-DG44) cell line. These amino acids were selected based on their relative mass fraction in the specific mAb produced in this study, their consumption rate during bioreactor experiments, and also through a literature review. A Plackett-Burman statistical design was conducted to minimize the number of experiments needed to obtain statistically relevant information. The effect of this set of amino acids was evaluated during exponential cell culture (considering viable cell concentration and the specific growth rate as main output variables) and during the high cell-density stage (considering mAb final concentration and specific productivity as relevant output variables). For this particular cell line, leucine (Leu) and arginine (Arg) had the highest negative and positive effects on cell viability, respectively; Leu and threonine (Thr) had the highest negative effect on growth rate, and valine (Val) and Arg demonstrated the highest positive impact on mAb final concentration. Results suggest the pertinence of a two-stage strategy for amino acid supplementation, with a mixture optimized for cell growth and a different amino acid mixture for mAb production at high density. Copyright © 2011 American Institute of Chemical Engineers (AIChE).

  19. Design of resilient consumer products

    DEFF Research Database (Denmark)

    Haug, Anders

    2016-01-01

    . In particular, the emotional perspective on product durability has received attention in recent design literature, since consumer products are often replaced long before they become physically non-functioning. However, the literature does not provide a full account of the causes of product replacement...

  20. Improving pension product design

    DEFF Research Database (Denmark)

    Konicz, Agnieszka Karolina; Mulvey, John M.

    2013-01-01

    , expected lifetime salary progression, mandatory and voluntary pension contributions, expected state retirement pension, choice of assets, subjective lifetime expectancy and bequest motive. Specically, the asset allocation strategy, the payout prole and the level of insured sum dening the new product...... are optimal under the expected utility function of retirement benets given the individual's characteristics. The problem is solved via a model that combines two optimization approaches: stochastic optimal control and multistage stochastic programming. The rst method is common in nancial and actuarial...... new thinking and add to actuarial practice....

  1. Mechatronics design principles for biotechnology product development.

    Science.gov (United States)

    Mandenius, Carl-Fredrik; Björkman, Mats

    2010-05-01

    Traditionally, biotechnology design has focused on the manufacture of chemicals and biologics. Still, a majority of biotechnology products that appear on the market today is the result of mechanical-electric (mechatronic) construction. For these, the biological components play decisive roles in the design solution; the biological entities are either integral parts of the design, or are transformed by the mechatronic system. This article explains how the development and production engineering design principles used for typical mechanical products can be adapted to the demands of biotechnology products, and how electronics, mechanics and biology can be integrated more successfully. We discuss three emerging areas of biotechnology in which mechatronic design principles can apply: stem cell manufacture, artificial organs, and bioreactors. Copyright 2010 Elsevier Ltd. All rights reserved.

  2. Production Facility SCADA Design Report

    Energy Technology Data Exchange (ETDEWEB)

    Dale, Gregory E. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Holloway, Michael Andrew [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Baily, Scott A. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Woloshun, Keith Albert [Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Wheat, Robert Mitchell Jr. [Los Alamos National Lab. (LANL), Los Alamos, NM (United States)

    2015-03-23

    The following report covers FY 14 activities to develop supervisory control and data acquisition (SCADA) system for the Northstar Moly99 production facility. The goal of this effort is to provide Northstar with a baseline system design.

  3. A BioDesign Approach to Obtain High Yields of Biosimilars by Anti-apoptotic Cell Engineering: a Case Study to Increase the Production Yield of Anti-TNF Alpha Producing Recombinant CHO Cells.

    Science.gov (United States)

    Gulce Iz, Sultan; Inevi, Muge Anil; Metiner, Pelin Saglam; Tamis, Duygu Ayyildiz; Kisbet, Nazli

    2018-01-01

    Recent developments in medical biotechnology have facilitated to enhance the production of monoclonal antibodies (mAbs) and recombinant proteins in mammalian cells. Human mAbs for clinical applications have focused on three areas, particularly cancer, immunological disorders, and infectious diseases. Tumor necrosis factor alpha (TNF-α), which has both proinflammatory and immunoregulatory functions, is an important target in biopharmaceutical industry. In this study, a humanized anti-TNF-α mAb producing stable CHO cell line which produces a biosimilar of Humira (adalimumab) was used. Adalimumab is a fully human anti-TNF mAb among the top-selling mAb products in recent years as a biosimilar. Products from mammalian cell bioprocesses are a derivative of cell viability and metabolism, which is mainly disrupted by cell death in bioreactors. Thus, different strategies are used to increase the product yield. Suppression of apoptosis, also called anti-apoptotic cell engineering, is the most remarkable strategy to enhance lifetime of cells for a longer production period. In fact, using anti-apoptotic cell engineering as a BioDesign approach was inspired by nature; nature gives prolonged life span to some cells like stem cells, tumor cells, and memory B and T cells, and researchers have been using this strategy for different purposes. In this study, as a biomimicry approach, anti-apoptotic cell engineering was used to increase the anti-TNF-α mAb production from the humanized anti-TNF-α mAb producing stable CHO cell line by Bcl-xL anti-apoptotic protein. It was shown that transient transfection of CHO cells by the Bcl-xL anti-apoptotic protein expressing plasmid prolonged the cell survival rate and protected cells from apoptosis. The transient expression of Bcl-xL using CHO cells enhanced the anti-TNF-α production. The production of anti-TNF-α in CHO cells was increased up to 215 mg/L with an increase of 160% after cells were transfected with Bcl-xL expressing plasmid

  4. Cork for sustainable product design

    NARCIS (Netherlands)

    Mestre, A.C.; Gil, L.

    2011-01-01

    Sustainable Product Design is currently accepted as one of the most promising trends in the “Sustainable Development” movement. It is often seen as a facilitation tool to implement Sustainability in practice, by improving the life cycle and eco-efficiency of products, by promoting dematerialization

  5. Cell production process

    OpenAIRE

    Salas Bacalla, Julio; FII-UNMSM

    2014-01-01

    The Cellular production consists on containing the components and machines in cells, combining the production for process with the online productión, to obtain the advantages  that offer both processes, in this case the company can offer variety of products and low costs. La producción celular, consiste en agrupar los componentes y máquinas en células, combinando la producción por proceso con la producción en línea, para obtener las ventajas que ofrecen ambos métodos, como son la variedad ...

  6. Total design for textile products

    Directory of Open Access Journals (Sweden)

    Zafirova Koleta

    2004-01-01

    Full Text Available Product development is less than 20-30 years old and a relatively new area of research compared to the other classic academic disciplines. Integrated product development is a philosophy that systematically employs the teaming of functional disciplines to integrate and concurrently apple all the necessary processes to produce an effective and efficient product that satisfies customer needs. Product development might also be understood as a multidisciplinary field of research. The disciplines directly participating in product development include engineering design, innovation, manufacturing, marketing and management. A background contribution is also generated by disciplines such as psychology, social sciences and information technology. This article is an overview that introduces this philosophy to textile product development.

  7. Operational, design and microbial aspects related to power production with microbial fuel cells implemented in constructed wetlands.

    Science.gov (United States)

    Corbella, Clara; Guivernau, Miriam; Viñas, Marc; Puigagut, Jaume

    2015-11-01

    This work aimed at determining the amount of energy that can be harvested by implementing microbial fuel cells (MFC) in horizontal subsurface constructed wetlands (HSSF CWs) during the treatment of real domestic wastewater. To this aim, MFC were implemented in a pilot plant based on two HSSF CW, one fed with primary settled wastewater (Settler line) and the other fed with the effluent of a hydrolytic up-flow sludge blanket reactor (HUSB line). The eubacterial and archaeal community was profiled on wetland gravel, MFC electrodes and primary treated wastewater by means of 16S rRNA gene-based 454-pyrosequencing and qPCR of 16S rRNA and mcrA genes. Maximum current (219 mA/m(2)) and power (36 mW/m(2)) densities were obtained for the HUSB line. Power production pattern correlated well with water level fluctuations within the wetlands, whereas the type of primary treatment implemented had a significant impact on the diversity and relative abundance of eubacteria communities colonizing MFC. It is worth noticing the high predominance (13-16% of relative abundance) of one OTU belonging to Geobacter on active MFC of the HUSB line that was absent for the settler line MFC. Hence, MFC show promise for power production in constructed wetlands receiving the effluent of a HUSB reactor. Copyright © 2015 Elsevier Ltd. All rights reserved.

  8. A glucose anode for enzymatic fuel cells optimized for current production under physiological conditions using a design of experiment approach.

    Science.gov (United States)

    Kumar, Rakesh; Leech, Dónal

    2015-12-01

    This study reports a design of experiment methodology to investigate and improve the performance of glucose oxidizing enzyme electrodes. Enzyme electrodes were constructed by co-immobilization of amine-containing osmium redox complexes, multiwalled carbon nanotubes and glucose oxidase in a carboxymethyldextran matrix at graphite electrode surfaces to provide a 3-dimensional matrix for electrocatalytic oxidation of glucose. Optimization of the amount of the enzyme electrode components to produce the highest current density under pseudo-physiological conditions of 5 mM glucose in saline buffer at 37 °C was performed using response surface methodology. A statistical analysis showed that the proposed model had a good fit with the experimental results. From the validated model, the addition of multiwalled carbon nanotubes and carboxymethyldextran components was identified as major contributing factors to the improved performance. Based on the optimized amount of components, enzyme electrodes display current densities of 1.2±0.1 mA cm(-2) and 5.2±0.2 mA cm(-2) at 0.2 V vs. Ag/AgCl in buffer containing 5 mM and 100 mM glucose, respectively, largely consistent with the predicted values. This demonstrates that use of a design of experiment approach can be applied effectively and efficiently to improve the performance of enzyme electrodes as anodes for biofuel cell device development. Copyright © 2015 Elsevier B.V. All rights reserved.

  9. Product design - Molecules, devices, functional products, and formulated products

    DEFF Research Database (Denmark)

    Gani, Rafiqul; Ng, Ka M.

    2015-01-01

    design is carried out by modeling the product based on thermodynamics, kinetics and transport processes, by performing experiments, and by decision making based on rule-based methods The results are product specifications in terms of the type of ingredients, composition, and the structure, form, shape...

  10. Energy-based culture medium design for biomanufacturing optimization: A case study in monoclonal antibody production by GS-NS0 cells.

    Science.gov (United States)

    Quiroga-Campano, Ana L; Panoskaltsis, Nicki; Mantalaris, Athanasios

    2018-03-02

    Demand for high-value biologics, a rapidly growing pipeline, and pressure from competition, time-to-market and regulators, necessitate novel biomanufacturing approaches, including Quality by Design (QbD) principles and Process Analytical Technologies (PAT), to facilitate accelerated, efficient and effective process development platforms that ensure consistent product quality and reduced lot-to-lot variability. Herein, QbD and PAT principles were incorporated within an innovative in vitro-in silico integrated framework for upstream process development (UPD). The central component of the UPD framework is a mathematical model that predicts dynamic nutrient uptake and average intracellular ATP content, based on biochemical reaction networks, to quantify and characterize energy metabolism and its adaptive response, metabolic shifts, to maintain ATP homeostasis. The accuracy and flexibility of the model depends on critical cell type/product/clone-specific parameters, which are experimentally estimated. The integrated in vitro-in silico platform and the model's predictive capacity reduced burden, time and expense of experimentation resulting in optimal medium design compared to commercially available culture media (80% amino acid reduction) and a fed-batch feeding strategy that increased productivity by 129%. The framework represents a flexible and efficient tool that transforms, improves and accelerates conventional process development in biomanufacturing with wide applications, including stem cell-based therapies. Copyright © 2018. Published by Elsevier Inc.

  11. Rapid thermal processing for production of chalcopyrite thin films for solar cells: Design, analysis, and experimental implementation

    Science.gov (United States)

    Lovelett, Robert J.

    The direct conversion of solar energy to electricity, or photovoltaic energy conversion, has a number of environmental, social, and economic advantages over conventional electricity generation from fossil fuels. Currently, the most commonly-used material for photovoltaics is crystalline silicon, which is now produced at large scale and silicon-based devices have achieved power conversion efficiencies over 25% However, alternative materials, such as inorganic thin films, offer a number of advantages including the potential for lower manufacturing costs, higher theoretical efficiencies, and better performance in the field. One of these materials is the chalcopyrite Cu(InGa)(SeS) 2, which has demonstrated module efficiencies over 17% and cell efficiencies over 22%. Cu(InGa)(SeS)2 is now in the early stages of commercialization using a precursor reaction process referred to as a "selenization/sulfization" reaction. The precursor reaction process is promising because it has demonstrated high efficiency along with the large area (approximately 1 m2) uniformity that is required for modules. However, some challenges remain that limit the growth of the chalcopyrite solar cell industry including: slow reactions that limit process throughput, a limited understanding of complex reaction kinetics and transport phenomena that affect the through-film composition, and the use of highly toxic H2Se in the reaction process. In this work, I approach each of these challenges. First, to improve process throughput, I designed and implemented a rapid thermal processing (RTP) reactor, whereby the samples are heated by a 1000 W quartz-halogen lamp that is capable of fast temperature ramps and high temperature dwells. With the reactor in place, however, achieving effective temperature control in the thin film material system is complicated by two intrinsic process characteristics: (i) the temperature of the Cu(InGa)(SeS)2 film cannot be measured directly, which leaves the system without

  12. Computational Intelligence Techniques for New Product Design

    CERN Document Server

    Chan, Kit Yan; Dillon, Tharam S

    2012-01-01

    Applying computational intelligence for product design is a fast-growing and promising research area in computer sciences and industrial engineering. However, there is currently a lack of books, which discuss this research area. This book discusses a wide range of computational intelligence techniques for implementation on product design. It covers common issues on product design from identification of customer requirements in product design, determination of importance of customer requirements, determination of optimal design attributes, relating design attributes and customer satisfaction, integration of marketing aspects into product design, affective product design, to quality control of new products. Approaches for refinement of computational intelligence are discussed, in order to address different issues on product design. Cases studies of product design in terms of development of real-world new products are included, in order to illustrate the design procedures, as well as the effectiveness of the com...

  13. By-products by design

    International Nuclear Information System (INIS)

    Collins, Richard

    2011-01-01

    Full text: Some mining by-products that are currently stockpiled or disposed of could be put to use preventing nutrients from entering river systems, helping reduce the potential for algal blooms. A joint project between CSIRO and the Western Australia (WA) Department of Water investigated a range of spent materials from mining and industry to determine their ability to filter nutrients from natural waters or to treat wastewater. “The largely unexploited by-product materials we generate in Western Australia could be developed as 'designer' contaminant adsorbents,” said CSIRO project leader Dr Grant Douglas. The use of abundant, low-cost wastes generated from mineral processing, in particular, offers a potentially cost- effective and environmentally-friendly strategy for removing nutrients. “The productive use of the by-products also has the potential to reduce the environmental footprint of mining and mineral processing industries by lowering by-product stockpiles,” he said. But the key benefits are in water; not only improving the health of surface waters but, by facilitating reuse, also easing the competition for water resources in an increasingly climate constrained part of the country. Re-use of industrial by-products in WA is currently considered on a case-by-case basis rather than regulated according to established standards. The project aimed to inject some rigour by characterising for the first time the nutrient, trace element uptake and acid neutralising capacity of a range of low-cost by-products (see Fact File). It also underpinned the development of a draft protocol for screening similar by-products in the future. The comprehensive characterisation included identification and procurement, and basic characterisation of by-products included major and trace element geochemistry, mineralogy, radioactivity, geochemical modelling and leachate chemistry and toxicity. These inherent properties and suitability of by-products for potential

  14. Strategies for fuel cell product development. Developing fuel cell products in the technology supply chain

    International Nuclear Information System (INIS)

    Hellman, H.L.

    2004-01-01

    Due to the high cost of research and development and the broad spectrum of knowledge and competences required to develop fuel cell products, many product-developing firms outsource fuel cell technology, either partly or completely. This article addresses the inter-firm process of fuel cell product development from an Industrial Design Engineering perspective. The fuel cell product development can currently be characterised by a high degree of economic and technical uncertainty. Regarding the technology uncertainty: product-developing firms are more often then not unfamiliar with fuel cell technology technology. Yet there is a high interface complexity between the technology supplied and the product in which it is to be incorporated. In this paper the information exchange in three current fuel cell product development projects is analysed to determine the information required by a product designer to develop a fuel cell product. Technology transfer literature suggests that transfer effectiveness is greatest when the type of technology (technology uncertainty) and the type of relationship between the technology supplier and the recipient are carefully matched. In this line of thinking this paper proposes that the information required by a designer, determined by the design strategy and product/system volume, should be met by an appropriate level of communication interactivity with a technology specialist. (author)

  15. Design of a single chambered microbial electrolytic cell reactor for production of biohydrogen from rice straw hydrolysate.

    Science.gov (United States)

    Gupta, Pratima; Parkhey, Piyush

    2015-06-01

    Rice straw was pretreated using a microwave-assisted alkali pretreatment method. Cellulose recovery was approximately 82 %. This material was hydrolysed in an optimized enzymatic saccharification reaction using cellulase from Lysinibacillus sphaericus. This resulted in saccharification of 49 % of cellulosic biomass into glucose. A single chambered microbial electrolytic cell reactor of volume 2l was built using acrylic plastic sheets with graphite sheet as anode and a stainless-steel mesh as cathode. Shewanella putrefaciens was used as exoelectrogen to oxidize rice straw hydrolysate in the reactor for electrohydrogenesis. The maximum H2 yield obtained was 801 ml H2 g(-1) COD removal. Coulombic efficiency of 88 %, cathodic H2 recovery of 58 % and total H2 recovery of 51 % with an energy efficiency of 74 % were recorded.

  16. Product Life Design: Notes for course 41627

    DEFF Research Database (Denmark)

    McAloone, Timothy Charles; Andreasen, Mogens Myrup

    2003-01-01

    to formulate specifications, synthesise and evaluate design proposals related to given product life systems. Training is also given in dealing with tools and principles for life cycle design (DFX). Course content The following aspects of life cycle design are considered: - Life cycle, stakeholders, resource......The course in product life design is designed to give an understanding of the phenomena of life cycle and product life thinking, the role of product design in fitting to product life systems and thus achieving sound product life perspectives. The course aims at developing an ability in the student......- and ecological specifications, life cycle-oriented quality, whole life costs. - Product life systems, "the meeting", relational properties, the role of the human operator. - Modelling of the product and the product life cycle, computer modelling. - The score model, Integrated Product Development, Life Cycle...

  17. Red blood cell production

    Science.gov (United States)

    ... bone marrow of bones. Stem cells in the red bone marrow called hemocytoblasts give rise to all of the formed elements in blood. If a hemocytoblast commits to becoming a cell called a proerythroblast, it will develop into a new red blood cell. The formation of a red blood ...

  18. Insights Gained from Testing Alternate Cell Designs

    International Nuclear Information System (INIS)

    O'Brien, J.E.; Stoots, C.M.; Herring, J.S.; Housley, G.K.; Sohal, M.S.; Milobar, D.G.; Cable, Thomas

    2009-01-01

    The Idaho National Laboratory (INL) has been researching the application of solid-oxide electrolysis cell for large-scale hydrogen production from steam over a temperature range of 800 to 900 C. The INL has been testing various solid oxide cell designs to characterize their electrolytic performance operating in the electrolysis mode for hydrogen production. Some results presented in this report were obtained from cells, initially developed by the Forschungszentrum Juelich and now manufactured by the French ceramics firm St. Gobain. These cells have an active area of 16 cm2 per cell. They were initially developed as fuel cells, but are being tested as electrolytic cells in the INL test stands. The electrolysis cells are electrode-supported, with ∼10 (micro)m thick yttria-stabilized zirconia (YSZ) electrolytes, ∼1400 (micro)m thick nickel-YSZ steam-hydrogen electrodes, and manganite (LSM) air-oxygen electrodes. The experiments were performed over a range of steam inlet mole fractions (0.1 to 0.6), gas flow rates, and current densities (0 to 0.6 A/cm2). Steam consumption rates associated with electrolysis were measured directly using inlet and outlet dewpoint instrumentation. On a molar basis, the steam consumption rate is equal to the hydrogen production rate. Cell performance was evaluated by performing DC potential sweeps at 800, 850, and 900 C. The voltage-current characteristics are presented, along with values of area-specific resistance as a function of current density. Long-term cell performance is also assessed to evaluate cell degradation. Details of the custom single-cell test apparatus developed for these experiments are also presented. NASA, in conjunction with the University of Toledo, has developed another fuel cell concept with the goals of reduced weight and high power density. The NASA cell is structurally symmetrical, with both electrodes supporting the thin electrolyte and containing micro-channels for gas diffusion. This configuration is

  19. CIRP Design 2012 Sustainable Product Development

    CERN Document Server

    2013-01-01

    During its life cycle, a product produces waste that is over 20 times its weight. As such it is critical to develop products that are sustainable. Currently product development processes lack high quality methods and tools that are empirically validated to support development of sustainable products. This book is a compilation of over forty cutting edge international research papers from the 22nd CIRP International Design Conference, written by eminent researchers from 15 countries, on engineering design process, methods and tools, broadly for supporting sustainable product development.   A variety of new insights into the product development process, as well as a host of methods and tools that are at the cutting edge of design research are discussed and explained covering a range of diverse topics. The areas covered include: ·Sustainable design and manufacturing, ·Design synthesis and creativity, ·Global product development and product life cycle management, ·Design for X (safety, reliability, manufactu...

  20. Mars oxygen production system design

    Science.gov (United States)

    Cotton, Charles E.; Pillow, Linda K.; Perkinson, Robert C.; Brownlie, R. P.; Chwalowski, P.; Carmona, M. F.; Coopersmith, J. P.; Goff, J. C.; Harvey, L. L.; Kovacs, L. A.

    1989-01-01

    The design and construction phase is summarized of the Mars oxygen demonstration project. The basic hardware required to produce oxygen from simulated Mars atmosphere was assembled and tested. Some design problems still remain with the sample collection and storage system. In addition, design and development of computer compatible data acquisition and control instrumentation is ongoing.

  1. Towards a theory of product design specifications

    DEFF Research Database (Denmark)

    Hansen, Claus Thorp; Andreasen, Mogens Myrup

    2004-01-01

    , which express the customers¿ need and perception of value. In this paper we will scrutinize this assumption. We outline the roles and tasks of the product design specification in order to identify the requirements on a theory of product design specifications and we identify existing theory elements......The product design specification is an important element in a product development project because it defines the target to be met. One requirement on the product design specification is to articulate and communicate the aspects, which makes the product attractive seen from the users' viewpoint....... In the design methodology literature we find guidelines and methods to compile a product design specification. The contributions are based on the common underlying assumption that it is meaningful and the only feasible approach to interpret the result of a need analysis into a set of technical specifications...

  2. CONCEPTUAL PRODUCT DESIGN IN VIRTUAL PROTOTYPING

    Directory of Open Access Journals (Sweden)

    Debeleac Carmen

    2009-07-01

    Full Text Available A conceptual model of the industrial design process for isolation against vibrations is proposed and described. This model can be used to design products subject to functional, manufacturing, ergonomic, aesthetic constraints. In this paper, the main stages of the model, such as component organization, conception shape, product detailing and graphical design are discussed. The work has confirmed the validity of proposed model for rapid generation of aesthetic preliminary product designs using the virtual prototyping technique, by one of its main component that is conceptual product design.

  3. A grand model for chemical product design

    DEFF Research Database (Denmark)

    Fung, Ka Y.; Ng, Ka M.; Zhang, Lei

    2016-01-01

    , ingredients and structure. Market and competitive analysis, government policies and regulations have to be explicitly considered in product design. All these considerations are accounted for in the Grand Product Design Model, which consists of a process model, a property model, a quality model, a cost model......Chemical engineering has been expanding its focus from primarily business-to-business products (B2B) to business-to-consumer (B2C) products. The production of B2B products generally emphasizes on process design and optimization, whereas the production of B2C products focuses on product quality...... product composition changes with market conditions. Another is a hand lotion that illustrates how product quality affects the profit.(C) 2016 Elsevier Ltd. All rights reserved....

  4. Production System Design for Multiple Brands

    DEFF Research Database (Denmark)

    Karlsson, Christer; Christensen, Irene

    appliances, cars, and trucks but also other products. An important denominator for the production system design is flow structures. Important dependent factors come from brand distinction, architectural characteristics, technology levels, supplier competence, process choice, process flow patterns, lay......-outs, worker skills, integration of distribution channels, after sales service and degree of servitization. Three production system design principles called VXY emerge....

  5. Advances in product family and product platform design methods & applications

    CERN Document Server

    Jiao, Jianxin; Siddique, Zahed; Hölttä-Otto, Katja

    2014-01-01

    Advances in Product Family and Product Platform Design: Methods & Applications highlights recent advances that have been made to support product family and product platform design and successful applications in industry. This book provides not only motivation for product family and product platform design—the “why” and “when” of platforming—but also methods and tools to support the design and development of families of products based on shared platforms—the “what”, “how”, and “where” of platforming. It begins with an overview of recent product family design research to introduce readers to the breadth of the topic and progresses to more detailed topics and design theory to help designers, engineers, and project managers plan, architect, and implement platform-based product development strategies in their companies. This book also: Presents state-of-the-art methods and tools for product family and product platform design Adopts an integrated, systems view on product family and pro...

  6. Holistic Design for Total Product Well Being

    Science.gov (United States)

    Adams, Chris W.; Hamilton, George S.

    2004-01-01

    Recent hardware development work at NASA's Marshall Space Flight Center creates and argument for the use of a holistic design approach as opposed to a piece part design approach. A piece part design approach being one where individual pieces are developed to their finished state having to meet certain interface and human engineering requirements without much consideration to the final product as a whole. A holistic design approach being one where the final product is evaluated early and frequently during the design process, and individual parts are developed with consideration to how they interact a whole,and how they interact with the user and environment. Examples from the development of the Materials Science Research Rack - 1 will illustrate: a design failure due to piece part design; a design save, due to a failure of piece part design, but saved by evaluating the design holistically; and a design success due to a holistic design approach.

  7. Design for the Environment Products (Online Search)

    Data.gov (United States)

    U.S. Environmental Protection Agency — This dataset contains a list of products that carry the Design for the Environment (DfE) label. This mark enables consumers to quickly identify and choose products...

  8. Design for the Environment Products (Raw Data)

    Data.gov (United States)

    U.S. Environmental Protection Agency — This dataset contains a list of products that carry the Design for the Environment (DfE) label. This mark enables consumers to quickly identify and choose products...

  9. Engineering Changes in Product Design - A Review

    Science.gov (United States)

    Karthik, K.; Janardhan Reddy, K., Dr

    2016-09-01

    Changes are fundamental to product development. Engineering changes are unavoidable and can arise at any phase of the product life cycle. The consideration of market requirements, customer/user feedbacks, manufacturing constraints, design innovations etc., turning them into viable products can be accomplished when product change is managed properly. In the early design cycle, informal changes are accepted. However, changes become formal when its complexity and cost increases, and as product matures. To maximize the market shares, manufacturers have to effectively and efficiently manage engineering changes by means of Configuration Control. The paper gives a broad overview about ‘Engineering Change Management’ (ECM) through configuration management and its implications in product design. The aim is to give an idea and understanding about the engineering changes in product design scenario to the new researchers. This paper elaborates the significant aspect of managing the engineering changes and the importance of ECM in a product life cycle.

  10. Improving product introduction through effective design reviews.

    Science.gov (United States)

    Pelnik, Tammy M

    2003-01-01

    The design review process is a part of the manufacturer's due diligence in developing a safe and effective product. Design review provides early and on-going independent feedback to developers. By adopting a proactive review process, design improvements can be pursued at an optimum time in the product development effort, i.e., when it will cost less to implement changes and when these changes may have the greatest impact. Effective implementation of the design review requirement will lead to better medical products and improved product introduction results.

  11. THE PRODUCT DESIGN PROCESS USING STYLISTIC SURFACES

    Directory of Open Access Journals (Sweden)

    Arkadiusz Gita

    2017-06-01

    Full Text Available The increasing consumer requirements for the way what everyday use products look like, forces manufacturers to put more emphasis on product design. Constructors, apart from the functional aspects of the parts created, are forced to pay attention to the aesthetic aspects. Software for designing A-class surfaces is very helpful in this case. Extensive quality analysis modules facilitate the work and allow getting models with specific visual features. The authors present a design process of the product using stylistic surfaces based on the front panel of the moped casing. In addition, methods of analysis of the design surface and product technology are presented.

  12. Buyer Search Costs and Endogenous Product Design

    OpenAIRE

    Dmitri Kuksov

    2004-01-01

    Buyer search costs for price are changing in many markets. Through a model of buyer and seller behavior, I consider the effects of changing search costs on prices both when product differentiation is fixed and when it is endogenously determined in equilibrium. If firms cannot change product design, lower buyer search costs for price lead to increased price competition. However, if product design is a decision variable, lower search costs for price may also lead to higher product differentiati...

  13. External designers in product design processes of small manufacturing firms

    NARCIS (Netherlands)

    Berends, Hans; Reymen, Isabelle; Stultiëns, Rutger G L; Peutz, Murk

    Small manufacturing firms often fail to reap the benefits of good design practices. This study investigates how the involvement of external designers influences the evolution of product design processes in small manufacturing firms. Qualitative and quantitative process research methods were used to

  14. Design Mining Microbial Fuel Cell Cascades

    OpenAIRE

    Preen, Richard J.; You, Jiseon; Bull, Larry; Ieropoulos, Ioannis A.

    2016-01-01

    Microbial fuel cells (MFCs) perform wastewater treatment and electricity production through the conversion of organic matter using microorganisms. For practical applications, it has been suggested that greater efficiency can be achieved by arranging multiple MFC units into physical stacks in a cascade with feedstock flowing sequentially between units. In this paper, we investigate the use of computational intelligence to physically explore and optimise (potentially) heterogeneous MFC designs ...

  15. Designing Visceral, Behavioural and Reflective Products

    Science.gov (United States)

    Aftab, Mersha; Rusli, Helen Agustin

    2017-09-01

    Designers and manufacturers often see consumption as the primary objective of a product - with implications such as discarded products, obsolete wastes, and ecological degradation. The paper aims to find the answer to the question, how emotional design can adapt the discarded and undesirable products into something valuable in a long term? This paper presents a framework combining Chapman's theory and Norman's theory on three levels of emotional design to highlight what long lasting connection with products entails. A design approach is presented combing the Wabi Sabi philosophy that promotes the celebration of decay and damage. This is used as one of the design principles for the experiments conducted on discarded products. Through constant user interaction before, during and after the experiments the evaluation of design as an agent of transformation is done. The user conducted the evaluation based on the Kansei elements of looks, sound, smell, and feel of the product. The experiments confirmed that a long-term value is only achieved through redesigning and reconstructing the perception of people towards products on a reflective level, rather than the visceral and behavioural elements of the product. The research found attachment to the visceral and behavioural elements of a product instead of an emotional one was causing users to discard products faster than required. The research indicated that many people, including designers and manufacturers, are unconsciously focusing on usability (behavioural level) and physical look (visceral level) of a product that are easily replaced, than on a meaningful way (reflective level) to create and maintain long-lasting emotions. The research concluded with a proposition towards digitization of products which could perhaps be an all round solution to make products more appropriate to human emotions. Digitization could give products the ability to capture, store and then communicate the stories, journey and memories back, in

  16. Design of experiments in production engineering

    CERN Document Server

    2016-01-01

    This book covers design of experiments (DoE) applied in production engineering as a combination of manufacturing technology with applied management science. It presents recent research advances and applications of design experiments in production engineering and the chapters cover metal cutting tools, soft computing for modelling and optmization of machining, waterjet machining of high performance ceramics, among others.

  17. Product Design Engineering--A Global Education Trend in Multidisciplinary Training for Creative Product Design

    Science.gov (United States)

    de Vere, Ian; Melles, Gavin; Kapoor, Ajay

    2010-01-01

    Product design is the convergence point for engineering and design thinking and practices. Until recently, product design has been taught either as a component of mechanical engineering or as a subject within design schools but increasingly there is global recognition of the need for greater synergies between industrial design and engineering…

  18. Does Design Activity Stimulate Firm Productivity? (Japanese)

    OpenAIRE

    KAWAKAMI Atsushi; EDAMURA Kazuma

    2015-01-01

    This paper investigates the influence of firms' design activity to total factor productivity using firm-level data from the Basic Survey of Japanese Business Structure and Activities and the Survey on Research Activities of Private Corporations. We adopt two approaches for calculating design activity. First, we regard registered design as a design activity. Second, we calculate design investment from the Survey on Research Activities of Private Corporations and estimate the relation between d...

  19. Integrating product design into the supply chain

    DEFF Research Database (Denmark)

    Khan, Omera; Stolte, Terje; Creazza, Alessandro

    2016-01-01

    into the supply chain. Originality/Value: This paper provides novel insights to both practitioners and researchers. For practitioners detailed recommendations are given on how they can maximise benefits through integrating product design into the supply chain. The RBV has been harnessed to highlight how......Purpose: The aim of the research is to illustrate how companies can create competitive capabilities through integration of product design into the supply chain. In doing so the paper reveals the challenges and the opportunities that companies face when integrating product design and supply chain...... of opportunities and challenges when integrating product design and the supply chain and subsequently a step-by-step guide is developed to address these. Practical Implications: The research provides key recommendations to companies on how to create competitive capabilities by integrating product design...

  20. Predifining Emotion Through Product Design

    OpenAIRE

    Reni, Theresia; Hudrasyah, Herry

    2010-01-01

    In the recent decades the business environment has changed tremendously due to the advance of globalization and competition, changing the essentials for success. To overcome competitive pressure, marketer and designer have to focus on customer's sensory needs and desires to create a deep rooted relationship through emotional dialogue. In this context, innovativeness appears to be a key ingredient to create and control consumer's emotion. Perceived of innovativeness is strongly infl...

  1. Consumer product innovation and sustainable design

    OpenAIRE

    Roy, Robin

    2015-01-01

    This paper summarises some findings of a book titled 'Consumer Product Innovation and Sustainable Design'. The book was inspired by the author's archive of the (UK) Consumers’ Association publication, Which? that provides a unique written and pictorial record of the technological and design evolution of consumer products marketed in Britain from 1957 to the present.\\ud \\ud The core of the book comprises case studies of six consumer product classes – bicycles, washing machines, electric lamps,...

  2. Description, Modelling and Design of Production Systems

    DEFF Research Database (Denmark)

    Jacobsen, Peter; Rudolph, Carsten

    1997-01-01

    Design of production systems are rarely an activity in which decision makers in most production companies have much experience. In future, this activity is to be more recurrent due to more and more frequent changes in the production task. Consequently, the decision makers are in need of better ma...

  3. Production System Design for Multiple Brands

    DEFF Research Database (Denmark)

    Karlsson, Christer; Christensen, Irene

    Production systems enabling both cost efficiency and flexibility in terms of high product variation are explored. The study follows an explorative longitudinal field study approach. The database consists of three large global corporations, each consisting of several companies producing household ......-outs, worker skills, integration of distribution channels, after sales service and degree of servitization. Three production system design principles called VXY emerge....

  4. Food product design. An integrated approach

    NARCIS (Netherlands)

    Linnemann, A.R.; Boekel, van M.A.J.S.

    2007-01-01

    This book explains how to apply barrier technology in food production to improve product stability and the possibilities of modelling and statistics in food product design are elaborated. Attention is given to Life Cycle Assessment as a method to determine the environmental impact of a food from

  5. Tools and techniques for product design

    NARCIS (Netherlands)

    Lutters, Diederick; Houten, Fred J.A.M.; Bernard, Alain; Mermoz, Emmanuel; Schutte, Corné S.L.

    2014-01-01

    For product designers, tools and techniques are essential in driving the design cycle. Nevertheless, their employment usually is implicit, while passing over e.g. the design and project environments empowering their adequate use. This publication presents an overview of approaches in structuring and

  6. Product design for cable waste peeler machine

    Science.gov (United States)

    Cahyadi, B.; Kurniawan, Y.

    2017-12-01

    The design of the cable waste peeler is a product design that aims to reduce the impact of health losses. In general, this is due to the activity of taking the metal in a cable that is done by burning the cable. The design process begins with the identification of customer needs. The customer referred to in this study is the informal sector. Based on interviews that have conducted on several respondents obtained four primary needs that must be illustrated in product design. Then, to explain the four needs are available three types of design variants. The results showed weighting measurements and calculations on load values of approximately 4.15 for the second variant. The results of these calculations are higher than the other two types of design variants. Thus, the design of the variant is chosen for the next design process.

  7. Design of formulated products: a systematic methodology

    DEFF Research Database (Denmark)

    Conte, Elisa; Gani, Rafiqul; Ng, K.M.

    2011-01-01

    -based computer-aided methodology for design and verification of a class of chemical-based products (liquid formulations) is presented. This methodology is part of an integrated three-stage approach for design/verification of liquid formulations where stage-1 generates a list of feasible product candidates and....../or verifies a specified set through a sequence of predefined activities (work-flow). Stage-2 and stage-3 (not presented here) deal with the planning and execution of experiments, for product validation. Four case studies have been developed to test the methodology. The computer-aided design (stage-1...

  8. [Strategic considerations on the design and choice of animal models for non-clinical investigations of cell-based medicinal products].

    Science.gov (United States)

    Lehmann, Jörg; Schulz, Ronny M; Sanzenbacher, Ralf

    2015-11-01

    For the development of medicinal products animal models are still indispensable to demonstrate efficacy and safety prior to first use in humans. Advanced therapy medicinal products (ATMP), which include cell-based medicinal products (CBMP), differ in their pharmacology and toxicology compared to conventional pharmaceuticals, and thus, require an adapted regime for non-clinical development. Developers are, therefore, challenged to develop particular individual concepts and to reconcile these with regulatory agencies. Guidelines issued by the European Medicines Agency (EMA), the U.S. Food and Drug Administration (FDA) and other sources can provide direction.The published approaches for non-clinical testing of efficacy document that homologous animal models where the therapeutic effect is investigated in a disease-relevant animal model utilizing cells derived from the same species are commonly used. The challenge is that the selected model should reflect the human disease in all critical features and that the cells should be comparable to the investigated human medicinal product in terms of quality and biological activity. This is not achievable in all cases. In these cases, alternative methods may provide supplemental information. To demonstrate the scientific proof-of-concept (PoC), small animal models such as mice or rats are preferred. During the subsequent product development phase, large animal models (i.e. sheep, minipigs, dogs) must be considered, as they may better reflect the anatomical or physiological situation in humans. In addition to efficacy, those models may also be suitable to prove some safety aspects of ATMP (e.g. regarding dose finding, local tolerance, or undesired interactions and effects of the administered cells in the target tissue). In contrast, for evaluation of the two prominent endpoints for characterizing the safety of ATMP (i.e. biodistribution, tumorigenicity) heterologous small animal models, especially immunodeficient mouse strains

  9. Health and Welfare IT Product Design

    DEFF Research Database (Denmark)

    Merritt, Timothy; Grønbæk, Kaj

    2012-01-01

    In this paper we describe the outcomes from a university design class focused on the design of systems intended to solve problems faced by health care professionals, patients and systems that support welfare or extended independence for older people. The students worked in groups for 10 weeks...... finding a suitable problem to solve, iterating and refining their designs, evaluating prototypes with users, and finally presenting their work as a potential product with a detailed plan for how the product would be sold in the market. The primary contribution of this paper is to share innovative design...

  10. Design of Formulated Products: Experimental Component

    DEFF Research Database (Denmark)

    Conte, Elisa; Gani, Rafiqul; Cheng, Y. S.

    2012-01-01

    : computer-aided design (Stage 1), which generates a list of feasible candidates, experimental planning (Stage 2), which generates a list of experiments and checks the available experimental set-ups, and experimental testing (Stage 3), which measures the necessary data and verifies the desirable attributes......A systematic methodology for the design and verification of chemical-based products is proposed. By integrating modeling, and experiments, the search space is efficiently scanned to identify the feasible product candidates. The product design (or verification) problem consists of three stages...... of the final product. The first stage (Stage 1) has been covered in previous publications, along with detailed case studies. The development of Stage 2 and Stage 3 is considered in this article and highlighted through two case studies involving the design and validation of an insect repellent lotion...

  11. Towards Generic Interaction Styles for Product Design

    DEFF Research Database (Denmark)

    Buur, Jacob; Stienstra, Marcelle

    2008-01-01

    A growing uneasiness among users with the experience of current product user interfaces mounts pressure on interaction designers to innovate user interface conventions. In previous research we have shown that a study of the history of product interaction triggers a broader discussion of interaction...... qualities among designers in a team, and that the naming of interaction styles helps establish an aesthetics of interaction design. However, that research focused on one particular product field, namely industrial controllers, and it was yet to be proven, if interaction styles do have generic traits across...... a wider range of interactive products. In this paper we report on five years of continued research into interaction styles for telephones, kitchen equipment, HiFi products and medical devices, and we show how it is indeed possible and beneficial to formulate a set of generic interaction styles....

  12. Prometheus Missing: Critical Materials and Product Design

    OpenAIRE

    Peck, David

    2016-01-01

    Critical Materials are strategically important to societies, businesses and economies. They are essential to maintain and improve our quality of life. The world however faces problems of critical material supply, but these concerns are not translated into product design activity, even though current policy and history suggests that product design can play a role in fi nding solutions to the critical materials problems. This thesis reviews literature from the past and draws observations from h...

  13. New design competences for localized production systems

    DEFF Research Database (Denmark)

    Eriksen, Kaare Riise; Morelli, Nicola

    2005-01-01

    This paper discusses the current development in production culture, based on local production networks. The role of the designer is put in perspective and outlined through a research project, Den Grønne Møbel (DGM), where different design areas were coordinated in a business concept...... and an organization involving different stakeholders. For several years now, DGM has been producing furniture from local materials, hereby aiming to create a new culture for well designed and environmentally sound furniture with a ‘story’. Although not totally new, this organizational model is particularly innovative...... in fully industrialized countries such as Denmark and represents an interesting alternative to globalized industrial production. Furthermore this case is an interesting opportunity to re-discuss the role and competences of designers in such new models. Tools and methods of the design profession...

  14. Haptics for Product Design and Manufacturing Simulation.

    Science.gov (United States)

    Xia, Pingjun

    2016-01-01

    Product design and manufacturing simulation is a promising research and application area for haptics. By benefiting from its natural human-computer interaction and realistic force/torque feedback, haptics can change the traditional design and manufacturing approaches which are mainly based on physical mock-ups or CAD (Computer Aided Design) modes. This paper provides a detailed and comprehensive survey of haptics for product design and manufacturing simulation in the past 10 years, mainly from 2004-2014, including haptics for product design and shape modelling, haptics for machining simulation, and haptics for virtual assembly and maintenance simulation. The new haptic devices and rendering algorithms involved in this area are introduced, the major research efforts and the typical systems are discussed, and the new ideas and research progresses are investigated. Then, conclusions and future trends are summarized.

  15. The Virtual Product-Process Design Laboratory for Structured Chemical Product Design and Analysis

    DEFF Research Database (Denmark)

    Mattei, Michele; Yunus, Nor Alafiza Binti; Kalakul, Sawitree

    2014-01-01

    the design and analysis of a wide range of homogeneous formulated products: tailor-made blends, single phase liquid formulations and emulsion-based products. The decision making process is supported by dedicated property models and structured databases, specifically developed for each design problem scenario......The objective of this paper is to present new methods for design of chemicals based formulated products and their implementation in the software, the Virtual Product-Process Design Laboratory. The new products are tailor-made blended liquid products and emulsion-based products. The new software...... employs a template approach, where each template follows the same common steps in the workflow for design of formulated products, but has the option to employ different product specific property models, data and calculation routines, when necessary. With the new additions, the software is able to support...

  16. Establishing elements of a synthetic biology platform for Vaccinia virus production: BioBrick™ design, serum-free virus production and microcarrier-based cultivation of CV-1 cells

    Directory of Open Access Journals (Sweden)

    Shuchang Liu

    2017-02-01

    Full Text Available Vaccinia virus (VACV is an established vector for vaccination and is beginning to prove effective as an oncolytic agent. Industrial production of VACV stands to benefit in future from advances made by synthetic biology in genome engineering and standardisation. The CV-1 cell line can be used for VACV propagation and has been used extensively with the CRISPR/Cas9 system for making precise edits of the VACV genome. Here we take first steps toward establishing a scalable synthetic biology platform for VACV production with CV-1 cells featuring standardised biological tools and serum free cell cultivation. We propose a new BioBrick™ plasmid backbone format for inserting transgenes into VACV. We then test the performance of CV-1 cells in propagation of a conventional recombinant Lister strain VACV, VACVL-15 RFP, in a serum-free process. CV-1 cells grown in 5% foetal bovine serum (FBS Dulbecco’s Modified Eagle Medium (DMEM were adapted to growth in OptiPRO and VP-SFM brands of serum-free media. Specific growth rates of 0.047 h−1 and 0.044 h−1 were observed for cells adapted to OptiPRO and VP-SFM respectively, compared to 0.035 h−1 in 5% FBS DMEM. Cells adapted to OptiPRO and to 5% FBS DMEM achieved recovery ratios of over 96%, an indication of their robustness to cryopreservation. Cells adapted to VP-SFM showed a recovery ratio of 82%. Virus productivity in static culture, measured as plaque forming units (PFU per propagator cell, was 75 PFU/cell for cells in 5% FBS DMEM. VP-SFM and OptiPRO adaptation increased VACV production to 150 PFU/cell and 350 PFU/cell respectively. Boosted PFU/cell from OptiPRO-adapted cells persisted when 5% FBS DMEM or OptiPRO medium was observed during the infection step and when titre was measured using cells adapted to 5% FBS DMEM or OptiPRO medium. Finally, OptiPRO-adapted CV-1 cells were successfully cultivated using Cytodex-1 microcarriers to inform future scale up studies.

  17. Establishing elements of a synthetic biology platform for Vaccinia virus production: BioBrick™ design, serum-free virus production and microcarrier-based cultivation of CV-1 cells.

    Science.gov (United States)

    Liu, Shuchang; Ruban, Ludmila; Wang, Yaohe; Zhou, Yuhong; Nesbeth, Darren N

    2017-02-01

    Vaccinia virus (VACV) is an established vector for vaccination and is beginning to prove effective as an oncolytic agent. Industrial production of VACV stands to benefit in future from advances made by synthetic biology in genome engineering and standardisation. The CV-1 cell line can be used for VACV propagation and has been used extensively with the CRISPR/Cas9 system for making precise edits of the VACV genome. Here we take first steps toward establishing a scalable synthetic biology platform for VACV production with CV-1 cells featuring standardised biological tools and serum free cell cultivation. We propose a new BioBrick™ plasmid backbone format for inserting transgenes into VACV. We then test the performance of CV-1 cells in propagation of a conventional recombinant Lister strain VACV, VACVL-15 RFP, in a serum-free process. CV-1 cells grown in 5% foetal bovine serum (FBS) Dulbecco's Modified Eagle Medium (DMEM) were adapted to growth in OptiPRO and VP-SFM brands of serum-free media. Specific growth rates of 0.047 h -1 and 0.044 h -1 were observed for cells adapted to OptiPRO and VP-SFM respectively, compared to 0.035 h -1 in 5% FBS DMEM. Cells adapted to OptiPRO and to 5% FBS DMEM achieved recovery ratios of over 96%, an indication of their robustness to cryopreservation. Cells adapted to VP-SFM showed a recovery ratio of 82%. Virus productivity in static culture, measured as plaque forming units (PFU) per propagator cell, was 75 PFU/cell for cells in 5% FBS DMEM. VP-SFM and OptiPRO adaptation increased VACV production to 150 PFU/cell and 350 PFU/cell respectively. Boosted PFU/cell from OptiPRO-adapted cells persisted when 5% FBS DMEM or OptiPRO medium was observed during the infection step and when titre was measured using cells adapted to 5% FBS DMEM or OptiPRO medium. Finally, OptiPRO-adapted CV-1 cells were successfully cultivated using Cytodex-1 microcarriers to inform future scale up studies.

  18. Product modular design incorporating preventive maintenance issues

    Science.gov (United States)

    Gao, Yicong; Feng, Yixiong; Tan, Jianrong

    2016-03-01

    Traditional modular design methods lead to product maintenance problems, because the module form of a system is created according to either the function requirements or the manufacturing considerations. For solving these problems, a new modular design method is proposed with the considerations of not only the traditional function related attributes, but also the maintenance related ones. First, modularity parameters and modularity scenarios for product modularity are defined. Then the reliability and economic assessment models of product modularity strategies are formulated with the introduction of the effective working age of modules. A mathematical model used to evaluate the difference among the modules of the product so that the optimal module of the product can be established. After that, a multi-objective optimization problem based on metrics for preventive maintenance interval different degrees and preventive maintenance economics is formulated for modular optimization. Multi-objective GA is utilized to rapidly approximate the Pareto set of optimal modularity strategy trade-offs between preventive maintenance cost and preventive maintenance interval difference degree. Finally, a coordinate CNC boring machine is adopted to depict the process of product modularity. In addition, two factorial design experiments based on the modularity parameters are constructed and analyzed. These experiments investigate the impacts of these parameters on the optimal modularity strategies and the structure of module. The research proposes a new modular design method, which may help to improve the maintainability of product in modular design.

  19. Conceptual frames for changing production system design

    DEFF Research Database (Denmark)

    Jensen, Per Langå; Broberg, Ole

    2003-01-01

    In order to integrate ergonomic aspects into the design of products and production systems many tools and procedures are available. For the ergonomists, however, it is a question of recognizing the organizational context and the role to play for making integration possible. Based on an understand...

  20. Lowlands sociotechnical design theory and lean production

    NARCIS (Netherlands)

    J.H.P. Christis; Erik Soepenberg

    2015-01-01

    Lean Production (LP) can be regarded as a design approach in search of a theoretical foundation. In this paper we show that Lowlands’ Sociotechnical Design Theory (STSL) could function as such a foundation. To reach this goal, we first describe STSL as a system theoretical reformulation of Original

  1. Bioreactor design for photofermentative hydrogen production.

    Science.gov (United States)

    Uyar, Basar

    2016-09-01

    Hydrogen will become a significant fuel in the near future. Photofermentative production of hydrogen is a promising and sustainable process. The design, construction and successful operation of the photobioreactors are of critical importance for photofermentative hydrogen production and became a major field of research where novel technologies are developed and adapted frequently. This paper gives an overview of the design aspects related to photobioreactors giving particular attention to design limitations, construction material, type, operating mode and scale-up. Sub-components of the overall system setup such as mixing, temperature control and hydrogen collection are also discussed. Recent achievements in the photobioreactor technologies are described.

  2. Production of oncolytic adenovirus and human mesenchymal stem cells in a single-use, Vertical-Wheel bioreactor system: Impact of bioreactor design on performance of microcarrier-based cell culture processes.

    Science.gov (United States)

    Sousa, Marcos F Q; Silva, Marta M; Giroux, Daniel; Hashimura, Yas; Wesselschmidt, Robin; Lee, Brian; Roldão, António; Carrondo, Manuel J T; Alves, Paula M; Serra, Margarida

    2015-01-01

    Anchorage-dependent cell cultures are used for the production of viruses, viral vectors, and vaccines, as well as for various cell therapies and tissue engineering applications. Most of these applications currently rely on planar technologies for the generation of biological products. However, as new cell therapy product candidates move from clinical trials towards potential commercialization, planar platforms have proven to be inadequate to meet large-scale manufacturing demand. Therefore, a new scalable platform for culturing anchorage-dependent cells at high cell volumetric concentrations is urgently needed. One promising solution is to grow cells on microcarriers suspended in single-use bioreactors. Toward this goal, a novel bioreactor system utilizing an innovative Vertical-Wheel™ technology was evaluated for its potential to support scalable cell culture process development. Two anchorage-dependent human cell types were used: human lung carcinoma cells (A549 cell line) and human bone marrow-derived mesenchymal stem cells (hMSC). Key hydrodynamic parameters such as power input, mixing time, Kolmogorov length scale, and shear stress were estimated. The performance of Vertical-Wheel bioreactors (PBS-VW) was then evaluated for A549 cell growth and oncolytic adenovirus type 5 production as well as for hMSC expansion. Regarding the first cell model, higher cell growth and number of infectious viruses per cell were achieved when compared with stirred tank (ST) bioreactors. For the hMSC model, although higher percentages of proliferative cells could be reached in the PBS-VW compared with ST bioreactors, no significant differences in the cell volumetric concentration and expansion factor were observed. Noteworthy, the hMSC population generated in the PBS-VW showed a significantly lower percentage of apoptotic cells as well as reduced levels of HLA-DR positive cells. Overall, these results showed that process transfer from ST bioreactor to PBS-VW, and scale-up was

  3. Safety in the design of production lines

    DEFF Research Database (Denmark)

    Dyhrberg, Mette Bang; Broberg, Ole; Jacobsen, Peter

    2006-01-01

    in the specification material nor in their face-to-face meetings with the supplier. Safety aspects were not part of their work practice. On this basis, it was suggested that formal guidelines or procedures for integrating safety in the design of production lines would have no effect. Instead, the researchers set up......This paper is a case study report on how safety considerations were handled in the process of redesigning a production line. The design process was characterized as a specification and negotiation process between engineers from the company and the supplier organization. The new production line...... became safer, but not as a result of any intentional plan to integrate safety aspects into the design process. Instead, the supplier’s design of a new piece of equipment had a higher built-in safety level. The engineering team in the company was aware of the importance of safety aspects neither...

  4. Tensor Product of Polygonal Cell Complexes

    OpenAIRE

    Chien, Yu-Yen

    2017-01-01

    We introduce the tensor product of polygonal cell complexes, which interacts nicely with the tensor product of link graphs of complexes. We also develop the unique factorization property of polygonal cell complexes with respect to the tensor product, and study the symmetries of tensor products of polygonal cell complexes.

  5. Effects of Polyhydroxybutyrate Production on Cell Division

    Science.gov (United States)

    Miller, Kathleen; Rahman, Asif; Hadi, Masood Z.

    2015-01-01

    Synthetic biological engineering can be utilized to aide the advancement of improved long-term space flight. The potential to use synthetic biology as a platform to biomanufacture desired equipment on demand using the three dimensional (3D) printer on the International Space Station (ISS) gives long-term NASA missions the flexibility to produce materials as needed on site. Polyhydroxybutyrates (PHBs) are biodegradable, have properties similar to plastics, and can be produced in Escherichia coli using genetic engineering. Using PHBs during space flight could assist mission success by providing a valuable source of biomaterials that can have many potential applications, particularly through 3D printing. It is well documented that during PHB production E. coli cells can become significantly elongated. The elongation of cells reduces the ability of the cells to divide and thus to produce PHB. I aim to better understand cell division during PHB production, through the design, building, and testing of synthetic biological circuits, and identify how to potentially increase yields of PHB with FtsZ overexpression, the gene responsible for cell division. Ultimately, an increase in the yield will allow more products to be created using the 3D printer on the ISS and beyond, thus aiding astronauts in their missions.

  6. Electronic cigarettes: product characterisation and design considerations.

    Science.gov (United States)

    Brown, Christopher J; Cheng, James M

    2014-05-01

    To review the available evidence regarding electronic cigarette (e-cigarette) product characterisation and design features in order to understand their potential impact on individual users and on public health. Systematic literature searches in 10 reference databases were conducted through October 2013. A total of 14 articles and documents and 16 patents were included in this analysis. Numerous disposable and reusable e-cigarette product options exist, representing wide variation in product configuration and component functionality. Common e-cigarette components include an aerosol generator, a flow sensor, a battery and a nicotine-containing solution storage area. e-cigarettes currently include many interchangeable parts, enabling users to modify the character of the delivered aerosol and, therefore, the product's 'effectiveness' as a nicotine delivery product. Materials in e-cigarettes may include metals, rubber and ceramics. Some materials may be aerosolised and have adverse health effects. Several studies have described significant performance variability across and within e-cigarette brands. Patent applications include novel product features designed to influence aerosol properties and e-cigarette efficiency at delivering nicotine. Although e-cigarettes share a basic design, engineering variations and user modifications result in differences in nicotine delivery and potential product risks. e-cigarette aerosols may include harmful and potentially harmful constituents. Battery explosions and the risks of exposure to the e-liquid (especially for children) are also concerns. Additional research will enhance the current understanding of basic e-cigarette design and operation, aerosol production and processing, and functionality. A standardised e-cigarette testing regime should be developed to allow product comparisons.

  7. Fuel cell systems and production aspects

    Energy Technology Data Exchange (ETDEWEB)

    Blomen, L.J.M.J. [Blomenco, Rumpt (Netherlands)

    1995-02-01

    Attention is paid to some system integration and production aspects of fuel cell systems. After an introduction of the main performance differences of fuel cell systems with different types of fuel cells, and the causes for such differences, a few aspects of building up sub-systems towards large and small capacity fuel cell systems are being discussed. Subsequently, some production aspects are being highlighted: the concept of an automated plant and automated plant contracting, the trends towards greater simplicity m reactors, and a systematic evaluation of the differences in fuel processing steps required in systems with different types of fuel cells. In this section also the cost aspects of a total plant contracting project are being discussed. As a next step, some emphasis has been placed on system integration aspects, explaining that hydrogen purification does not always `destroy` system efficiency, and can be used in dispersed hydrogen generation concepts. Advanced system integration concepts include simplified flow sheet design, in which instead of special dedicated rotating equipment standard gas turbine rotary components are being employed. As an example, it is claimed that gas turbine - fuel cell combinations can be made with very high electrical efficiencies. 5 figs., 2 tabs.

  8. Production engineering jig and tool design

    CERN Document Server

    Jones, E J H

    1972-01-01

    Production Engineering: Jig and Tool Design focuses on jig and tool design as part of production engineering and covers topics ranging from inspection and gauging to multiple and consecutive tooling, tool calculation and development of form tools, deep-hole boring, and grinding-wheel form-crushing. Air and oil operated fixtures, negative rake machining, and the economics of jig and fixture practice are also discussed. This text is comprised of 22 chapters; the first of which provides an overview of the function and organization of the jig and tool department. Attention then turns to the subjec

  9. Soft and hard product attributes in design

    DEFF Research Database (Denmark)

    Lenau, Torben Anker; Boelskifte, Per

    2003-01-01

    , business and engineering) showed consensus on assigning certain words to specific products in order to express the sensory and perceived experiences (Johnson et al. 2003). An initial vocabulary was formulated based on input from the design literature. The study showed that a significant amount of the test...... group agreed on assigning the same words to 6 specific products. Based on the input from the study the initial vocabulary was revised....

  10. Design, product structuring and modelling of mechatronic products and systems

    DEFF Research Database (Denmark)

    Conrad, Finn; Sørensen, Torben

    2003-01-01

    Information Technology offers software and hardware for improvement of the engineering design, structuring and control systems, and industrial applications. The latest progress in IT makes integration of an overall design and manufacturing IT- concept feasible and commercially attractive. An IT......-tool concept for modelling, simulation and design of mechatronic products and systems is proposed in this paper. It built on results from a Danish mechatronic research program on intelligent motion control as well as from the Esprit project SWING on IT-tools for rapid prototyping of fluid power components...

  11. PLUTONIUM-238 PRODUCTION TARGET DESIGN STUDIES

    Energy Technology Data Exchange (ETDEWEB)

    Hurt, Christopher J [ORNL; Wham, Robert M [ORNL; Hobbs, Randall W [ORNL; Owens, R Steven [ORNL; Chandler, David [ORNL; Freels, James D [ORNL; Maldonado, G Ivan [ORNL

    2014-01-01

    A new supply chain is planned for plutonium-238 using existing reactors at the Oak Ridge National Laboratory (ORNL) and Idaho National Laboratory (INL) and existing chemical recovery facilities at ORNL. Validation and testing activities for new irradiation target designs have been conducted in three phases over a 2 year period to provide data for scale-up to production. Target design, qualification, target fabrication, and irradiation of fully-loaded targets have been accomplished. Data from post-irradiation examination (PIE) supports safety analysis and irradiation of future target designs.

  12. Prometheus Missing : Critical Materials and Product Design

    NARCIS (Netherlands)

    Peck, David

    2016-01-01

    Critical Materials are strategically important to societies, businesses and economies. They are essential to maintain and improve our quality of life. The world however faces problems of critical material supply, but these concerns are not translated into product design activity, even though current

  13. Design of environmentally friendly products using indicators

    DEFF Research Database (Denmark)

    Lenau, Torben Anker; Bey, Niki

    2001-01-01

    The paper describes the requirements of product designers towards methods for environmental evaluation; it argues that indicators can be used for rough environmental evaluations and it presents experiences made with the indicator-based Oil Point Method (OPM). This method is illustrated by means...

  14. Product design engineering - a global education trend in multidisciplinary training for creative product design

    Science.gov (United States)

    de Vere, Ian; Melles, Gavin; Kapoor, Ajay

    2010-03-01

    Product design is the convergence point for engineering and design thinking and practices. Until recently, product design has been taught either as a component of mechanical engineering or as a subject within design schools but increasingly there is global recognition of the need for greater synergies between industrial design and engineering training. Product design engineering (PDE) is a new interdisciplinary programme combining the strengths of the industrial design and engineering. This paper examines the emergence of PDE in an environment of critique of conventional engineering education and exemplifies the current spread of programmes endorsing a hybrid programme of design and engineering skills. The paper exemplifies PDE with the analysis of the programme offered at Swinburne University of Technology (Australia), showing how the teaching of 'designerly' thinking to engineers produces a new graduate particularly suited to the current and future environment of produce design practice. The paper concludes with reflections on the significance of this innovative curriculum model for the field of product design and for engineering design in general.

  15. Thermal design of a Mars oxygen production plant

    Science.gov (United States)

    Sridhar, K. R.; Iyer, Venkatesh A.

    1991-01-01

    The optimal design of the thermal components of a system that uses carbon dioxide from the Martian atmosphere to produce oxygen for spacecraft propulsion and/or life support is discussed. The gases are pressurized, heated and passed through an electrochemical cell. Carbon dioxide is reduced to carbon monoxide and oxygen due to thermal dissociation and electrocatalysis. The oxygen thus formed is separated from the gas mixture by the electrochemical cell. The objective of the design is to optimize both the overall mass and the power consumption of the system. The analysis shows that at electrochemical cell efficiencies of about 50 percent and lower, the optimal system would require unspent carbon dioxide in the exhaust gases to be separated and recycled. Various methods of efficiently compressing the intake gases to system pressures of 0.1 MPa are investigated. The total power requirement for oxygen production rates of 1, 5, and 10 kg/day at various cell efficiencies are presented.

  16. Digital Production and Students as Learning Designers

    DEFF Research Database (Denmark)

    Sørensen, Birgitte Holm; Levinsen, Karin

    2014-01-01

    Today’s digitalization allows users to interact, collaborate, communicate and create user-generated content. The technology is intuitive and easy to use even for young children, and new learning opportunities emerge. Particularly, students’ production as a learning form benefits from digitalization...... as the new opportunities enable young students to integrate their playing competencies and skills into the formal school learning. This paper presents and discusses a theory regarding students’ digital production from a learning and design-for-learning perspective, which is generated based on the project...

  17. Raman spectroscopy in pharmaceutical product design

    DEFF Research Database (Denmark)

    Paudel, Amrit; Raijada, Dhara; Rantanen, Jukka

    2015-01-01

    molecular-based drug discovery, design of innovative drug delivery systems and quality control of finished products. This review presents concise accounts of various conventional and emerging Raman instrumentations including associated hyphenated tools of pharmaceutical interest. Moreover, relevant...... application cases of Raman spectroscopy in early and late phase pharmaceutical development, process analysis and micro-structural analysis of drug delivery systems are introduced. Finally, potential areas of future advancement and application of Raman spectroscopic techniques are discussed....

  18. URPD: a specific product primer design tool

    Directory of Open Access Journals (Sweden)

    Chuang Li-Yeh

    2012-06-01

    Full Text Available Abstract Background Polymerase chain reaction (PCR plays an important role in molecular biology. Primer design fundamentally determines its results. Here, we present a currently available software that is not located in analyzing large sequence but used for a rather straight-forward way of visualizing the primer design process for infrequent users. Findings URPD (yoUR Primer Design, a web-based specific product primer design tool, combines the NCBI Reference Sequences (RefSeq, UCSC In-Silico PCR, memetic algorithm (MA and genetic algorithm (GA primer design methods to obtain specific primer sets. A friendly user interface is accomplished by built-in parameter settings. The incorporated smooth pipeline operations effectively guide both occasional and advanced users. URPD contains an automated process, which produces feasible primer pairs that satisfy the specific needs of the experimental design with practical PCR amplifications. Visual virtual gel electrophoresis and in silico PCR provide a simulated PCR environment. The comparison of Practical gel electrophoresis comparison to virtual gel electrophoresis facilitates and verifies the PCR experiment. Wet-laboratory validation proved that the system provides feasible primers. Conclusions URPD is a user-friendly tool that provides specific primer design results. The pipeline design path makes it easy to operate for beginners. URPD also provides a high throughput primer design function. Moreover, the advanced parameter settings assist sophisticated researchers in performing experiential PCR. Several novel functions, such as a nucleotide accession number template sequence input, local and global specificity estimation, primer pair redesign, user-interactive sequence scale selection, and virtual and practical PCR gel electrophoresis discrepancies have been developed and integrated into URPD. The URPD program is implemented in JAVA and freely available at http://bio.kuas.edu.tw/urpd/.

  19. Shaping Dreams: Design Ideas and Design Fiction in Movie and Television Production Design

    DEFF Research Database (Denmark)

    Ion Wille, Jakob

    2015-01-01

    practitioners value the contribution of designers in developing the narrative through visual support. The paper suggests a comparison of design and screenplay research and analysis. In addition, it touches on the subject of design fiction in the case of a project involving collaboration of production design......The aim of this article is to show how the design process influences the planning and development of moving images, i.e., live-action movies, animation, and television. The paper documents the significance of design in the early stages of film and television production and shows how industry...

  20. Transitions in Sustainable Product Design Research

    DEFF Research Database (Denmark)

    Boks, Casper; McAloone, Tim C.

    2009-01-01

    , this research area has expanded considerably; from a bunch of opportunistic eco-pathfinders trying to make products better recyclable into acknowledged scientific research regarding technology transfer and commercialisation. This paper proposes that this maturing process took place through a number......By the early 1990s, sustainable product innovation (or ecodesign, or Design for environment) had gained sufficient critical mass in academic research to be identified as a distinct research area. In the past 15 years, stimulated by a growing environmental concern and awareness in the media...... of transitions; this is illustrated by discussing characteristic aspects of each transition, which together provide a historic account of how academic research into sustainable product innovation had matured. In conclusion, a number of possible future transitions or extensions of the research area are discussed....

  1. Product Design – From Molecules to Formulations to Devices

    DEFF Research Database (Denmark)

    Gani, Rafiqul; Ng, Ka M.

    2014-01-01

    Product design is a multidisciplinary and diverse subject. This article focuses on product conceptualization (what-to-make) and briefly reviews the corresponding manufacturing processes (how-to-make). Four product types are considered – molecular products, formulated products, devices and functio......Product design is a multidisciplinary and diverse subject. This article focuses on product conceptualization (what-to-make) and briefly reviews the corresponding manufacturing processes (how-to-make). Four product types are considered – molecular products, formulated products, devices...

  2. FUEL CELLS IN ENERGY PRODUCTION

    OpenAIRE

    Huang, Xiaoyu

    2011-01-01

    The purpose of this thesis is to study fuel cells. They convert chemical energy directly into electrical energy with high efficiency and low emmission of pollutants. This thesis provides an overview of fuel cell technology.The basic working principle of fuel cells and the basic fuel cell system components are introduced in this thesis. The properties, advantages, disadvantages and applications of six different kinds of fuel cells are introduced. Then the efficiency of each fuel cell is p...

  3. Defining process design space for monoclonal antibody cell culture.

    Science.gov (United States)

    Abu-Absi, Susan Fugett; Yang, LiYing; Thompson, Patrick; Jiang, Canping; Kandula, Sunitha; Schilling, Bernhard; Shukla, Abhinav A

    2010-08-15

    The concept of design space has been taking root as a foundation of in-process control strategies for biopharmaceutical manufacturing processes. During mapping of the process design space, the multidimensional combination of operational variables is studied to quantify the impact on process performance in terms of productivity and product quality. An efficient methodology to map the design space for a monoclonal antibody cell culture process is described. A failure modes and effects analysis (FMEA) was used as the basis for the process characterization exercise. This was followed by an integrated study of the inoculum stage of the process which includes progressive shake flask and seed bioreactor steps. The operating conditions for the seed bioreactor were studied in an integrated fashion with the production bioreactor using a two stage design of experiments (DOE) methodology to enable optimization of operating conditions. A two level Resolution IV design was followed by a central composite design (CCD). These experiments enabled identification of the edge of failure and classification of the operational parameters as non-key, key or critical. In addition, the models generated from the data provide further insight into balancing productivity of the cell culture process with product quality considerations. Finally, process and product-related impurity clearance was evaluated by studies linking the upstream process with downstream purification. Production bioreactor parameters that directly influence antibody charge variants and glycosylation in CHO systems were identified.

  4. A product design framework for a circular economy

    NARCIS (Netherlands)

    Van den Berg, M.R.; Bakker, C.A.

    2015-01-01

    The paper provides a circular economy framework from a product design perspective with tools to aid product designers in applying circular product design in practice. Design research for circular economy has so far mainly been limited to referring to existing fields of research such as design for

  5. Design for high productivity remote handling

    International Nuclear Information System (INIS)

    Sykes, N.; Collins, S.; Loving, A.B.; Ricardo, V.; Villedieu, E.

    2011-01-01

    As the central part of a programme of enhancements in support of ITER, the Joint European Torus (JET) is being equipped with an all-metal wall. This enhancement programme requires the removal and installation of 6927 tile carriers and tiles, as well as the removal and installation of embedded diagnostics and antennas. The scale of this operation and the necessity to maximise operational availability of the facility added a requirement for high productivity in the remote activities to the existing exigencies of precision, reliability, cleanliness and operational security. This high productivity requirement has been incorporated into the design of the components and associated installation tooling, the design of the installation equipment, the development of installation procedures including the use of a mock-up for optimisation and training. Consideration of the remote handling installation process is vital during the design of the in vessel components. A number of features to meet the need of the high productivity while maintaining the function requirements have been incorporated into the metal wall components and associated tooling including kinematic design with guidance appropriate for remote operation. The component and tools are designed to guide the attachment of the installation tool, the installation path, and the interlocking with adjacent components without contact between the fragile castellated beryllium of the adjacent tiles. Other incorporated ergonomic features are discussed. At JET, the remote maintenance is conducted using end effectors, normally bi-lateral force feed back manipulator, mounted on driven, articulated booms. Prior to the current shutdown one long boom was used to conduct the installation and collect and deliver components to the 'short' boom which was linked to the tile carrier transfer facility. This led to loss of efficiency during these movements. The adoption of a new remote handling philosophy using 'point of installation

  6. Audio-visual interactions in product sound design

    NARCIS (Netherlands)

    Özcan, E.; Van Egmond, R.

    2010-01-01

    Consistent product experience requires congruity between product properties such as visual appearance and sound. Therefore, for designing appropriate product sounds by manipulating their spectral-temporal structure, product sounds should preferably not be considered in isolation but as an integral

  7. The metabolic productivity of the cell factory.

    Science.gov (United States)

    Cascante, M; Lloréns, M; Meléndez-Hevia, E; Puigjaner, J; Montero, F; Martí, E

    1996-10-07

    It is widely accepted that some performance function has been optimized during the evolution of metabolic pathways. One can study the nature of such a function by analogy with the industrial manufacturing world, in which there have been efforts over recent decades to optimize production chains, and in which it is now accepted that fluxes are not the only important system variables that determine process efficiency, because inventory turnover must also be considered. Inspired by the parallels between living cells and manufacturing factories, we propose that fluxes and transit time may have simultaneously been major targets of natural selection in the optimization of the design, structure and kinetic parameters of metabolic pathways. Accordingly we define the ratio of flux to transit time as a performance index of productivity in metabolic systems: it measures the efficiency with which stocks are administered, and facilitates comparison of a pathway in different steady states or in different tissues or organisms. For a linear chain of two enzymes, at a fixed total equilibrium constant, we have analysed the variation of flux, transit time and productivity index as functions of the equilibrium constants of the two steps. The results show that only the productivity index has a maximum, which represents a good compromise in optimizing flux and transit time. We have extended control analysis to the productivity index and derived the summation theorem that applies to it. For linear chains of different length with maximum productivity index values, the distribution of control coefficients with regard to the three parameters has a characteristic profile independent of the length of the chain. Finally, this control profile changes when other variables are optimized, and we compare the theoretical results with the control profile of the first steps of glycolysis in rat liver.

  8. Fuel cells principles, design, and analysis

    CERN Document Server

    Revankar, Shripad T

    2014-01-01

    ""This book covers all essential themes of fuel cells ranging from fundamentals to applications. It includes key advanced topics important for understanding correctly the underlying multi-science phenomena of fuel cell processes. The book does not only cope with traditional fuel cells but also discusses the future concepts of fuel cells. The book is rich on examples and solutions important for applying the theory into practical use.""-Peter Lund, Aalto University, Helsinki""A good introduction to the range of disciplines needed to design, build and test fuel cells.""-Nigel Brandon, Imperial Co

  9. Electrochemical characterisation of solid oxide cell electrodes for hydrogen production

    DEFF Research Database (Denmark)

    Bernuy-Lopez, Carlos; Knibbe, Ruth; He, Zeming

    2011-01-01

    Oxygen electrodes and steam electrodes are designed and tested to develop improved solid oxide electrolysis cells for H2 production with the cell support on the oxygen electrode. The electrode performance is evaluated by impedance spectroscopy testing of symmetric cells at open circuit voltage (OCV...... and impregnated with CGO/Ni, with an Rp of 0.08 Ω cm2 at 850 °C in 3% H2O/H2....

  10. Product design pattern based on big data-driven scenario

    OpenAIRE

    Conggang Yu; Lusha Zhu

    2016-01-01

    This article discusses about new product design patterns in the big data era, gives designer a new rational thinking way, and is a new way to understand the design of the product. Based on the key criteria of the product design process, category, element, and product are used to input the data, which comprises concrete data and abstract data as an enlargement of the criteria of product design process for the establishment of a big data-driven product design pattern’s model. Moreover, an exper...

  11. Intelligent Product Construction and Design in Smart Earth

    Science.gov (United States)

    Zhou, Xi; Li, Qiao

    2017-08-01

    Under the concept of intelligent earth development, smart products should be born.Smart product building is not the technology of traditional products,but high-tech products to give perception, memory, thinking, reflecting a series of intellectual ability.Its design thinking is more systematic, more macroscopic, more stereo.Smart product design challenges the wisdom of mankind.

  12. Design of metallic bipolar plates for PEM fuel cells.

    Science.gov (United States)

    2012-01-01

    This project focused on the design and production of metallic bipolar plates for use in PEM fuel cells. Different metals were explored : and stainless steel was found out to be best suited to our purpose. Following the selection of metal, it was calc...

  13. Product design for elderly-visual design information inspired a new perspective in design education

    NARCIS (Netherlands)

    Langeveld, L.H.

    2014-01-01

    A workshop Product Design for Elderly was held in Beijing and organized by the School of Digital Media and Design, Beijing University of Posts and Telecommunications. A domestic appliances company had sponsored a part of the workshop and brought in the topics. The objectives of the workshop were

  14. Integrate System Modeling for Design and Production Planning of High Quality Products Considering Failure Data

    Science.gov (United States)

    Aoyama, Kazuhiro; Koga, Tsuyoshi

    2009-07-01

    Since the product recall problem is recently observed, realizing design and production activities that can prevent occurrences of a product failure has been becoming a serious issue. This paper proposes "the Synthetic design approach of a product and a production process which enables to reduce occurrences of a product failure from the initial stage of a product development." In order to realize this proposed concept as a specific system, the integrated model of the failure information in the design and production is introduced. This paper shows some examples of design and production for a circuit breaker and an automobile with considering a design error and a production failure using developed prototype system.

  15. Improved vanillin production in baker's yeast through in silico design

    DEFF Research Database (Denmark)

    Brochado, Ana Rita; Matos, Cláudia; Møller, Birger L.

    2010-01-01

    Background: Vanillin is one of the most widely used flavouring agents, originally obtained from cured seed pods of the vanilla orchid Vanilla planifolia. Currently vanillin is mostly produced via chemical synthesis. A de novo synthetic pathway for heterologous vanillin production from glucose has...... recently been implemented in baker's yeast, Saccharamyces cerevisiae. In this study we aimed at engineering this vanillin cell factory towards improved productivity and thereby at developing an attractive alternative to chemical synthesis. Results: Expression of a glycosyltransferase from Arabidopsis...... thaliana in the vanillin producing S. cerevisiae strain served to decrease product toxicity. An in silico metabolic engineering strategy of this vanillin glucoside producing strain was designed using a set of stoichiometric modelling tools applied to the yeast genome-scale metabolic network. Two targets...

  16. Mammalian designer cells: Engineering principles and biomedical applications.

    Science.gov (United States)

    Xie, Mingqi; Fussenegger, Martin

    2015-07-01

    Biotechnology is a widely interdisciplinary field focusing on the use of living cells or organisms to solve established problems in medicine, food production and agriculture. Synthetic biology, the science of engineering complex biological systems that do not exist in nature, continues to provide the biotechnology industry with tools, technologies and intellectual property leading to improved cellular performance. One key aspect of synthetic biology is the engineering of deliberately reprogrammed designer cells whose behavior can be controlled over time and space. This review discusses the most commonly used techniques to engineer mammalian designer cells; while control elements acting on the transcriptional and translational levels of target gene expression determine the kinetic and dynamic profiles, coupling them to a variety of extracellular stimuli permits their remote control with user-defined trigger signals. Designer mammalian cells with novel or improved biological functions not only directly improve the production efficiency during biopharmaceutical manufacturing but also open the door for cell-based treatment strategies in molecular and translational medicine. In the future, the rational combination of multiple sets of designer cells could permit the construction and regulation of higher-order systems with increased complexity, thereby enabling the molecular reprogramming of tissues, organisms or even populations with highest precision. Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  17. Photovoltaic cell and production thereof

    Science.gov (United States)

    Narayanan, Srinivasamohan [Gaithersburg, MD; Kumar, Bikash [Bangalore, IN

    2008-07-22

    An efficient photovoltaic cell, and its process of manufacture, is disclosed wherein the back surface p-n junction is removed from a doped substrate having an oppositely doped emitter layer. A front surface and edges and optionally the back surface periphery are masked and a back surface etch is performed. The mask is not removed and acts as an anti-reflective coating, a passivating agent, or both. The photovoltaic cell retains an untextured back surface whether or not the front is textured and the dopant layer on the back surface is removed to enhance the cell efficiency. Optionally, a back surface field is formed.

  18. Design and Control of High Temperature PEM Fuel Cell System

    DEFF Research Database (Denmark)

    Andreasen, Søren Juhl

    demands for this. A 1kW fuel cell stack with optimized  ow plates was heated in 5 minutes using the introduction of an electrical air pre-heater. Using pure hydrogen in compressed form is problematic due to the very small density of hydrogen, even at high pressures. Hydrogen is a very energy e-cient gas...... enables the use of designing cathode air cooled stacks greatly simplifying the fuel cell system and lowering the parasitic losses. Furthermore, the fuel impurity tolerance is signicantly improved because of the higher temperatures, and much higher concentrations of CO can be endured without performance...... or life time losses. In order to evaluate the performance of using HTPEM fuel cells for electricity production in electrical applications, a 400 W fuel cell system is initially designed using a cathode air cooled 30 cell HTPEM stack. The stack runs on pure hydrogen in a deadend anode configuration...

  19. Scale-up of cell culture bioreactors using biomechatronic design.

    Science.gov (United States)

    Mandenius, Carl-Fredrik; Björkman, Mats

    2012-08-01

    Scale-up of cell culture bioreactors is a challenging engineering work that requires wide competence in cell biology, mechanical engineering and bioprocess design. In this article, a new approach for cell culture bioreactor scale-up is suggested that is based on biomechatronic design methodology. The approach differs from traditional biochemical engineering methodology by applying a sequential design procedure where the needs of the users and alternative design solutions are systematically analysed. The procedure is based on the biological and technical functions of the scaled-up bioreactor that are derived in functional maps, concept generation charts and scoring and interaction matrices. Basic reactor engineering properties, such as mass and heat transfer and kinetics are integrated in the procedure. The methodology results in the generation of alternative design solutions that are thoroughly ranked with help of the user needs. Examples from monoclonal antibodies and recombinant protein production illuminate the steps of the procedure. The methodology provides engineering teams with additional tools that can significantly facilitate the design of new production methods for cell culture processes. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  20. Embedded systems engineering for products and services design.

    Science.gov (United States)

    Ahram, Tareq Z; Karwowski, Waldemar; Soares, Marcelo M

    2012-01-01

    Systems engineering (SE) professionals strive to develop new techniques to enhance the value of contributions to multidisciplinary smart product design teams. Products and services designers challenge themselves to search beyond the traditional design concept of addressing the physical, social, and cognitive factors. This paper covers the application of embedded user-centered systems engineering design practices into work processes based on the ISO 13407 framework [20] to support smart systems and services design and development. As practitioners collaborate to investigate alternative smart product designs, they concentrate on creating valuable products which will enhance positive interaction. This paper capitalizes on the need to follow a user-centered SE approach to smart products design [4, 22]. Products and systems intelligence should embrace a positive approach to user-centered design while improving our understanding of usable value-adding, experience and extending our knowledge of what inspires others to design enjoyable services and products.

  1. Harnessing cell-material interaction to control cell fate: design ...

    Indian Academy of Sciences (India)

    Rajat K Das

    2017-10-26

    Oct 26, 2017 ... advancement in engineered hydrogel materials as such scaffold to control cell fate. Keywords. Extracellular matrix; hydrogel .... vided useful model systems to identify principles that would help design synthetic ..... tool to generate nanoscale periodic patterns that influ- enced stem cell differentiation.

  2. Harnessing cell-material interaction to control cell fate: design ...

    Indian Academy of Sciences (India)

    Rajat K Das

    2017-10-26

    Oct 26, 2017 ... Harnessing cell-material interaction to control cell fate: design principle of advanced functional hydrogel materials. †. RAJAT K DAS. ∗. Materials Science Centre, Indian Institute of Technology, Kharagpur, West Bengal, India. E-mail: rajat@matsc.iitkgp.ernet.in. MS received 15 August 2017; revised 29 ...

  3. Flow Cell Design for Effective Biosensing

    Science.gov (United States)

    Pike, Douglas J.; Kapur, Nikil; Millner, Paul A.; Stewart, Douglas I.

    2013-01-01

    The efficiency of three different biosensor flow cells is reported. All three flow cells featured a central channel that expands in the vicinity of the sensing element to provide the same diameter active region, but the rate of channel expansion and contraction varied between the designs. For each cell the rate at which the analyte concentration in the sensor chamber responds to a change in the influent analyte concentration was determined numerically using a finite element model and experimentally using a flow-fluorescence technique. Reduced flow cell efficiency with increasing flow rates was observed for all three designs and was related to the increased importance of diffusion relative to advection, with efficiency being limited by the development of regions of recirculating flow (eddies). However, the onset of eddy development occurred at higher flow rates for the design with the most gradual channel expansion, producing a considerably more efficient flow cell across the range of flow rates considered in this study. It is recommended that biosensor flow cells be designed to minimize the tendency towards, and be operated under conditions that prevent the development of flow recirculation. PMID:23344373

  4. A Systematic Optimization Design Method for Complex Mechatronic Products Design and Development

    OpenAIRE

    Jiang, Jie; Ding, Guofu; Zhang, Jian; Zou, Yisheng; Qin, Shengfeng

    2018-01-01

    Designing a complex mechatronic product involves multiple design variables, objectives, constraints, and evaluation criteria as well as their nonlinearly coupled relationships. The design space can be very big consisting of many functional design parameters, structural design parameters, and behavioral design (or running performances) parameters. Given a big design space and inexplicit relations among them, how to design a product optimally in an optimization design process is a challenging r...

  5. The Current of Continuing Professional Development for Product Designers

    OpenAIRE

    山内, 勉; Yamauchi, Tsutomu

    2010-01-01

    Product Designers are required some skills and knowledge in the product development. Based on my professional career, I presumed three skills, they are, Technical skills, Conceptual skills and Human skills. In this study, I interviewed some Product Designers to make sure what skills are needed in the product development process. It emerged that Product Designers are expected to improve their Conceptual and Human skills besides Technical skills for playing a part at the upper and lower stage o...

  6. 47 CFR 7.7 - Product design, development, and evaluation.

    Science.gov (United States)

    2010-10-01

    ... usability as part of such a product design and development process. (b) In developing such a process... 47 Telecommunication 1 2010-10-01 2010-10-01 false Product design, development, and evaluation. 7...? § 7.7 Product design, development, and evaluation. (a) Manufacturers and service providers shall...

  7. 36 CFR 1193.23 - Product design, development, and evaluation.

    Science.gov (United States)

    2010-07-01

    ... product design and development process. (b) In developing such a process, manufacturers shall consider the... 36 Parks, Forests, and Public Property 3 2010-07-01 2010-07-01 false Product design, development... § 1193.23 Product design, development, and evaluation. (a) Manufacturers shall evaluate the accessibility...

  8. Designer rhamnolipids by reduction of congener diversity: production and characterization.

    Science.gov (United States)

    Tiso, Till; Zauter, Rabea; Tulke, Hannah; Leuchtle, Bernd; Li, Wing-Jin; Behrens, Beate; Wittgens, Andreas; Rosenau, Frank; Hayen, Heiko; Blank, Lars Mathias

    2017-12-14

    Rhamnolipids are biosurfactants featuring surface-active properties that render them suitable for a broad range of industrial applications. These properties include their emulsification and foaming capacity, critical micelle concentration, and ability to lower surface tension. Further, aspects like biocompatibility and environmental friendliness are becoming increasingly important. Rhamnolipids are mainly produced by pathogenic bacteria like Pseudomonas aeruginosa. We previously designed and constructed a recombinant Pseudomonas putida KT2440, which synthesizes rhamnolipids by decoupling production from host-intrinsic regulations and cell growth. Here, the molecular structure of the rhamnolipids, i.e., different congeners produced by engineered P. putida are reported. Natural rhamnolipid producers can synthesize mono- and di-rhamnolipids, containing one or two rhamnose molecules, respectively. Of each type of rhamnolipid four main congeners are produced, deviating in the chain lengths of the β-hydroxy-fatty acids. The resulting eight main rhamnolipid congeners with variable numbers of hydrophobic/hydrophilic residues and their mixtures feature different physico-chemical properties that might lead to diverse applications. We engineered a microbial cell factory to specifically produce three different biosurfactant mixtures: a mixture of di- and mono-rhamnolipids, mono-rhamnolipids only, and hydroxyalkanoyloxy alkanoates, the precursors of rhamnolipid synthesis, consisting only of β-hydroxy-fatty acids. To support the possibility of second generation biosurfactant production with our engineered microbial cell factory, we demonstrate rhamnolipid production from sustainable carbon sources, including glycerol and xylose. A simple purification procedure resulted in biosurfactants with purities of up to 90%. Finally, through determination of properties specific for surface active compounds, we were able to show that the different mixtures indeed feature different physico

  9. Physical Properties for Lipids Based Process and Product Design

    DEFF Research Database (Denmark)

    Ana Perederic, Olivia; Kalakul, Sawitree; Sarup, Bent

    Lipid processing covers several oil and fats technologies such as: edible oil production, biodieselproduction, oleochemicals (e.g.: food additives, detergents) and pharmaceutical product manufacturing. New demands regarding design and development of better products and more sustainable processes...

  10. New Vistas in Chemical Product and Process Design

    DEFF Research Database (Denmark)

    Zhang, Lei; Babi, Deenesh Kavi; Gani, Rafiqul

    2016-01-01

    , its corresponding process, and its integration are highlighted. Although significant advances have been made in the development of systematic model-based techniques for process design (also for optimization, operation, and control), much work is needed to reach the same level for product design....... Timeline diagrams illustrating key contributions in product design, process design, and integrated product-process design are presented. The search for novel, innovative, and sustainable solutions must be matched by consideration of issues related to the multidisciplinary nature of problems, the lack......Design of chemicals-based products is broadly classified into those that are process centered and those that are product centered. In this article, the designs of both classes of products are reviewed from a process systems point of view; developments related to the design of the chemical product...

  11. Scale-Up of World Record 16.5% CdTe Cell Design for a 50 MWp Production Facility: September 27, 2007 - March 26, 2009

    Energy Technology Data Exchange (ETDEWEB)

    Seymour, F. H.

    2011-05-01

    This final report covers progress made on subcontract NAT-7-77015-07 by PrimeStar Solar Inc. for the 18 month period from September 27, 2007 through March 26, 2009. The project objectives were to accelerate the commercialization of the world record 16.5% efficiency cadmium telluride photovoltaic technology. This was done by developing high performance 6"x6" prototype mini-modules and by designing and commissioning a pilot line for manufacturing of commercial sized 60cmx120cm modules. This subcontract aligns with the SAI program by accelerating the development of high efficiency low cost CdTe solar PV module manufacturing. This will contribute to the goal of PV grid parity by 2015. Progress with the deliverables and milestones in this subcontract constitutes progress towards the KPP and milestone objectives for the SAI program.

  12. Improved vanillin production in baker's yeast through in silico design.

    Science.gov (United States)

    Brochado, Ana Rita; Matos, Claudia; Møller, Birger L; Hansen, Jørgen; Mortensen, Uffe H; Patil, Kiran Raosaheb

    2010-11-08

    Vanillin is one of the most widely used flavouring agents, originally obtained from cured seed pods of the vanilla orchid Vanilla planifolia. Currently vanillin is mostly produced via chemical synthesis. A de novo synthetic pathway for heterologous vanillin production from glucose has recently been implemented in baker's yeast, Saccharamyces cerevisiae. In this study we aimed at engineering this vanillin cell factory towards improved productivity and thereby at developing an attractive alternative to chemical synthesis. Expression of a glycosyltransferase from Arabidopsis thaliana in the vanillin producing S. cerevisiae strain served to decrease product toxicity. An in silico metabolic engineering strategy of this vanillin glucoside producing strain was designed using a set of stoichiometric modelling tools applied to the yeast genome-scale metabolic network. Two targets (PDC1 and GDH1) were selected for experimental verification resulting in four engineered strains. Three of the mutants showed up to 1.5 fold higher vanillin β-D-glucoside yield in batch mode, while continuous culture of the Δpdc1 mutant showed a 2-fold productivity improvement. This mutant presented a 5-fold improvement in free vanillin production compared to the previous work on de novo vanillin biosynthesis in baker's yeast. Use of constraints corresponding to different physiological states was found to greatly influence the target predictions given minimization of metabolic adjustment (MOMA) as biological objective function. In vivo verification of the targets, selected based on their predicted metabolic adjustment, successfully led to overproducing strains. Overall, we propose and demonstrate a framework for in silico design and target selection for improving microbial cell factories.

  13. Improved vanillin production in baker's yeast through in silico design

    Directory of Open Access Journals (Sweden)

    Hansen Jørgen

    2010-11-01

    Full Text Available Abstract Background Vanillin is one of the most widely used flavouring agents, originally obtained from cured seed pods of the vanilla orchid Vanilla planifolia. Currently vanillin is mostly produced via chemical synthesis. A de novo synthetic pathway for heterologous vanillin production from glucose has recently been implemented in baker's yeast, Saccharamyces cerevisiae. In this study we aimed at engineering this vanillin cell factory towards improved productivity and thereby at developing an attractive alternative to chemical synthesis. Results Expression of a glycosyltransferase from Arabidopsis thaliana in the vanillin producing S. cerevisiae strain served to decrease product toxicity. An in silico metabolic engineering strategy of this vanillin glucoside producing strain was designed using a set of stoichiometric modelling tools applied to the yeast genome-scale metabolic network. Two targets (PDC1 and GDH1 were selected for experimental verification resulting in four engineered strains. Three of the mutants showed up to 1.5 fold higher vanillin β-D-glucoside yield in batch mode, while continuous culture of the Δpdc1 mutant showed a 2-fold productivity improvement. This mutant presented a 5-fold improvement in free vanillin production compared to the previous work on de novo vanillin biosynthesis in baker's yeast. Conclusion Use of constraints corresponding to different physiological states was found to greatly influence the target predictions given minimization of metabolic adjustment (MOMA as biological objective function. In vivo verification of the targets, selected based on their predicted metabolic adjustment, successfully led to overproducing strains. Overall, we propose and demonstrate a framework for in silico design and target selection for improving microbial cell factories.

  14. Improved vanillin production in baker's yeast through in silico design

    Science.gov (United States)

    2010-01-01

    Background Vanillin is one of the most widely used flavouring agents, originally obtained from cured seed pods of the vanilla orchid Vanilla planifolia. Currently vanillin is mostly produced via chemical synthesis. A de novo synthetic pathway for heterologous vanillin production from glucose has recently been implemented in baker's yeast, Saccharamyces cerevisiae. In this study we aimed at engineering this vanillin cell factory towards improved productivity and thereby at developing an attractive alternative to chemical synthesis. Results Expression of a glycosyltransferase from Arabidopsis thaliana in the vanillin producing S. cerevisiae strain served to decrease product toxicity. An in silico metabolic engineering strategy of this vanillin glucoside producing strain was designed using a set of stoichiometric modelling tools applied to the yeast genome-scale metabolic network. Two targets (PDC1 and GDH1) were selected for experimental verification resulting in four engineered strains. Three of the mutants showed up to 1.5 fold higher vanillin β-D-glucoside yield in batch mode, while continuous culture of the Δpdc1 mutant showed a 2-fold productivity improvement. This mutant presented a 5-fold improvement in free vanillin production compared to the previous work on de novo vanillin biosynthesis in baker's yeast. Conclusion Use of constraints corresponding to different physiological states was found to greatly influence the target predictions given minimization of metabolic adjustment (MOMA) as biological objective function. In vivo verification of the targets, selected based on their predicted metabolic adjustment, successfully led to overproducing strains. Overall, we propose and demonstrate a framework for in silico design and target selection for improving microbial cell factories. PMID:21059201

  15. Product quality driven food process design

    NARCIS (Netherlands)

    Hadiyanto, M.

    2007-01-01

    Consumers evaluate food products on their quality, and thus the product quality is a main target in industrial food production. In the last decade there has been a remarkable increase of interest of the food industry to put food product quality central in innovation. However, quality itself is

  16. The rules of unruly product design

    NARCIS (Netherlands)

    Eggink, Wouter; Roozenburg, N.; Chen, L.L.; Stappers, P.J.

    2011-01-01

    This paper is part of a research into the history of unruly design, which aims at finding theoretical background for the design of everyday things in a postmodern society. Unruly design is defined in this research as: all objects that are designed with the intention to undermine the existing

  17. Design of Sustainable Blended Products using an Integrated Methodology

    DEFF Research Database (Denmark)

    Yunus, Nor Alafiza Binti; Gernaey, Krist; Woodley, John

    2013-01-01

    This paper presents a systematic methodology for designing blended products consisting of three stages; product design, process identification and experimental verification. The product design stage is considered in this paper. The objective of this stage is to screen and select suitable chemicals...... to be used as building blocks in the mixture design, and then to propose the blend formulations that fulfill the desired product attributes. The result is a set of blends that match the constraints, the compositions, values of the target properties and information about their miscibility. The methodology has...... been applied to design several blended products. A case study on design of blended lubricants is highlighted. The objective is to identify blended products that satisfy the product attributes with at least similar or better performance compared to conventional products....

  18. Improvement of product design process by knowledge value analysis

    OpenAIRE

    XU, Yang; BERNARD, Alain; PERRY, Nicolas; LAROCHE, Florent

    2013-01-01

    Nowadays, design activities remain the core issue for global product development. As knowledge is more and more integrated, effective analysis of knowledge value becomes very useful for the improvement of product design processes. This paper aims at proposing a framework of knowledge value analysis in the context of product design process. By theoretical analysis and case study, the paper illustrates how knowledge value can be calculated and how the results can help the improvement of product...

  19. Plant Design for the Production of DUAGG

    International Nuclear Information System (INIS)

    Ferrada, J.J.

    2003-01-01

    The cost of producing DUAGG is an important consideration for any interested private firm in determining whether DUCRETE is economically viable as a material of construction in next-generation spent nuclear fuel casks. This study analyzed this project as if it was a stand-alone project. The capital cost includes engineering design, equipment costs and installation, start up, and management; the study is not intended to be a life-cycle cost analysis. The costs estimated by this study are shown in Table ES.1, and the conclusions of this study are listed in Table ES.2. The development of DUAGG and DUCRETE is a major thrust of the Depleted Uranium Uses Research and Development Project. An obvious use of depleted uranium is as a shielding material (e.g., DUCRETE). DUCRETE is made by replacing the conventional stone aggregate in concrete with DUAGG. One objective of this project is to bring the development of DUCRETE to a point at which a demonstrated basis exists for its commercial deployment. The estimation of the costs to manufacture DUAGG is an important part of this effort. Paul Lessing and William Quapp developed DUAGG and DUCRETE as part of an Idaho National Engineering and Environmental Laboratory (INEEL) program to find beneficial uses for depleted uranium (DU). Subsequently, this technology was licensed to Teton Technologies, Inc. The DUAGG process mixes DUO 2 with sintering materials and additives to form pressed briquettes. These briquettes are sintered at 1300 C, and the very dense sintered briquettes are then crushed and classified into gap-graded size fractions. The graded DUAGG is then ready to be used to make high-strength heavy DUCRETE. The DUCRETE shielding will be placed into an annular steel cask-shell mold, which has internal steel reinforcing bars. The objectives of this study are to (1) use previous DUAGG process developments to design a plant that will produce DUAGG at a baseline rate, (2) determine the size of the equipment required to meet the

  20. Interactive Multimedia Design and Production Processes.

    Science.gov (United States)

    Liu, Min; Jones, Colleen; Hemstreet, Susan

    1998-01-01

    A study involving interviews with experienced educational multimedia developers examined multimedia production, professional roles and responsibilities, factors contributing to successful multimedia development, production tips and techniques from practitioners, and hardware and software. Article discusses implications of the findings and makes…

  1. Development and design of micro products

    DEFF Research Database (Denmark)

    Hansen, Hans Nørgaard

    2004-01-01

    Innovation within the field of micro and nano technology is characterized by cross-disciplinary skills The IT-sector driven development of materials/technologies related to semiconductors and silicon The more traditional materials polymers, metals, ceramics: well suited for mass production...... but not widespread in micro products today New possibilities with expensive materials in micro products....

  2. High-Volume Production of Lightweight Multijunction Solar Cells

    Science.gov (United States)

    Youtsey, Christopher

    2015-01-01

    MicroLink Devices, Inc., has transitioned its 6-inch epitaxial lift-off (ELO) solar cell fabrication process into a manufacturing platform capable of sustaining large-volume production. This Phase II project improves the ELO process by reducing cycle time and increasing the yield of large-area devices. In addition, all critical device fabrication processes have transitioned to 6-inch production tool sets designed for volume production. An emphasis on automated cassette-to-cassette and batch processes minimizes operator dependence and cell performance variability. MicroLink Devices established a pilot production line capable of at least 1,500 6-inch wafers per month at greater than 80 percent yield. The company also increased the yield and manufacturability of the 6-inch reclaim process, which is crucial to reducing the cost of the cells.

  3. Experimental design methods for fermentative hydrogen production: A review

    Energy Technology Data Exchange (ETDEWEB)

    Wang, Jianlong; Wan, Wei [Laboratory of Environmental Technology, INET, Tsinghua University, Beijing 100084 (China)

    2009-01-15

    This review summarized the experimental design methods used to investigate the effects of various factors on fermentative hydrogen production processes, including one-factor-at-a-time design, full factorial design, Taguchi design, Plackett-Burman design, central composite design and Box-Behnken design. Each design method was briefly introduced, followed by the introduction of its analysis. In addition, the advantages and disadvantages of each design method were briefly discussed. Moreover, the application of each design method to the study of fermentative hydrogen production was analyzed and discussed. Based on the discussion in this review, an experimental design strategy for optimizing fermentative hydrogen production processes was proposed. In the end, the software packages that can carry out the above mentioned factorial design and analysis were briefly introduced. (author)

  4. A Contradiction-Based Approach for Innovative Product Design

    Directory of Open Access Journals (Sweden)

    Ko Yao-Tsung

    2016-01-01

    Full Text Available Without creativity in design there is no potential for innovation. This paper investigates the role of contradictions in enhancing creativity in product design. Based on the inventive principles of TRIZ, this paper presents a novel design method by integrating technical and physical contradiction analysis methods into the conceptual design activities of new product development (NPD. Despite the recognized importance of innovative design, there is a lack of the systematic and effective design-thinking process that can covers all conceptual design activities. To address this gap, a sharper and fundamental model of the problem-solving is created for innovative product design based on the contradiction-oriented concept. Eventually, one case study is employed to illustrate the method and the result validates that it can help designers produce more creative outcomes in product design.

  5. Cork Design : A Design Action Intervention Approach Towards Sustainable Product Innovation

    NARCIS (Netherlands)

    Mestre, A.C.C.M.

    2014-01-01

    The study Cork Design: A Design Action Intervention Approach Towards Sustainable Product Innovation comprises the systematic implementation of sustainable product innovation within the Portuguese cork sector, through action research. Cork is a natural, recyclable, non-toxic, and renewable resource,

  6. Viral antigen production in cell cultures on microcarriers Bovine parainfluenza 3 virus and MDBK cells.

    Science.gov (United States)

    Conceição, M M; Tonso, A; Freitas, C B; Pereira, C A

    2007-11-07

    Viral antigens can be obtained from infected mammalian cells cultivated on microcarriers. We have worked out parameters for the production of bovine parainfluenza 3 (PI-3) virus by Mandin-Darby Bovine Kidney (MDBK) cells cultivated on Cytodex 1 microcarriers (MCs) in spinners flasks and bioreactor using fetal bovine serum (FBS) supplemented Eagle minimal essential medium (Eagle-MEM). Medium renewal during the cell culture was shown to be crucial for optimal MCs loading (>90% MCs with confluent cell monolayers) and cell growth (2.5 x 10(6)cells/mL and a micro(x) (h(-1)) 0.05). Since cell cultures performed with lower amount of MCs (1g/L), showed good performances in terms of cell loading, we designed batch experiments with a lower concentration of MCs in view of optimizing the cell growth and virus production. Studies of cell growth with lower concentrations of MCs (0.85 g/L) showed that an increase in the initial cell seeding (from 7 to 40 cells/MC) led to a different kinetic of initial cell growth but to comparable final cell concentrations ((8-10)x10(5)cells/mL at 120 h) and cell loading (210-270 cells/MC). Upon infection with PI-3 virus, cultures showed a decrease in cell growth and MC loading directly related to the multiplicity of infection (moi) used for virus infection. Infected cultures showed also a higher consumption of glucose and production of lactate. The PI-3 virus and PI-3 antigen production among the cultures was not significantly different and attained values ranging from, respectively, 7-9 log(10) TCID(50)/mL and 1.5-2.2 OD. The kinetics of PI-3 virus production showed a sharp increase during the first 24h and those of PI-3 antigen increased after 24h. The differential kinetics of PI-3 virus and PI-3 antigen can be explained by the virus sensitivity to temperature. In view of establishing a protocol of virus production and based on the previous experiments, MDBK cell cultures performed under medium perfusion in a bioreactor of 1.2L were infected

  7. Property Modelling for Applications in Chemical Product and Process Design

    DEFF Research Database (Denmark)

    Gani, Rafiqul

    Physical-chemical properties of pure chemicals and their mixtures play an important role in the design of chemicals based products and the processes that manufacture them. Although, the use of experimental data in design and analysis of chemicals based products and their processes is desirable...... such as database, property model library, model parameter regression, and, property-model based product-process design will be presented. The database contains pure component and mixture data for a wide range of organic chemicals. The property models are based on the combined group contribution and atom...... modeling tools in design and analysis of chemical product-process design, including biochemical processes will be highlighted....

  8. Bovine mammary stem cells: Cell biology meets production agriculture

    Science.gov (United States)

    Mammary stem cells (MaSC) provide for net growth, renewal and turnover of mammary epithelial cells, and are therefore potential targets for strategies to increase production efficiency. Appropriate regulation of MaSC can potentially benefit milk yield, persistency, dry period management and tissue ...

  9. Expression regulation of design process gene in product design

    DEFF Research Database (Denmark)

    Li, Bo; Fang, Lusheng; Li, Bo

    2011-01-01

    is proposed and analyzed, as well as its three categories i.e., the operator gene, the structural gene and the regulator gene. Second, the trigger mechanism that design objectives and constraints trigger the operator gene is constructed. Third, the expression principle of structural gene is analyzed...... with the example of design management gene. Last, the regulation mode that the regulator gene regulates the expression of the structural gene is established and it is illustrated by taking the design process management gene as an example. © (2011) Trans Tech Publications....

  10. Simulation-based comparisons of four apparel cell production modes of one clothing production line

    Directory of Open Access Journals (Sweden)

    Guoqiang Pan

    2014-10-01

    Full Text Available Purpose: This research, by using the SIMIO simulation platform, provides a quantitative and comparative analysis of how the efficiency of four different cell production modes is affected. It is hoped that the outcomes will be of some help for garment factories to optimize their production lines. Design/methodology/approach: The SIMIO simulation platform was employed in the research and comparisons were made of the simulation test results about the four different production modes. Findings: The operation mode, number of operators, and number of buffer areas are key factors affecting the production line efficiency, and need to be reasonably set to achieve the highest efficiency. Originality/value: As most research literature so far is qualitative, this research provided a simulation-based quantitative analysis of the production efficiency under different cell production modes.

  11. Nature-Inspired Design : Strategies for Sustainable Product Development

    NARCIS (Netherlands)

    De Pauw, I.C.

    2015-01-01

    Product designers can apply different strategies, methods, and tools for sustainable product development. Nature-Inspired Design Strategies (NIDS) offer designers a distinct class of strategies that use ‘nature’ as a guiding source of knowledge and inspiration for addressing sustainability.

  12. Teaching User-Centered Design in New Product Marketing

    Science.gov (United States)

    Love, Edwin; Stone, Donn E.; Wilton, Taine

    2011-01-01

    Thanks in part to groundbreaking work by companies such as Apple and IDEO, there has been growing interest in design as a way to improve the odds of new product success. This paper describes a user-centered design workshop developed for a new product marketing course. The workshop included exercises designed to explain and illustrate the…

  13. Design for manufacturability of macro and micro products: a case study of heat exchanger design

    DEFF Research Database (Denmark)

    Omidvarnia, F.; Weng Feng, L.; Hansen, H. N.

    2018-01-01

    In this paper, a novel methodology in designing a micro heat exchanger is proposed by modifying a conventional design methodology for macro products with the considerations of differences between design of a micro and a macro product. The methodology starts with the identification of differences...... by designers/engineers in design of the same category of micro products. Furthermore, through utilization of the proposed methodology by designers/engineers for design of other micro products, the RTC unit can be enriched with micro-oriented design principles and accordingly provide a basic guideline...... in design considerations for micro scale products compared to the macro scale. These design considerations consist of material selection, manufacturing process, physical phenomena and shape and geometry design. Manufacturability criteria are defined and various potential manufacturing processes...

  14. Fungal protein production: Design and production of chimeric proteins

    NARCIS (Netherlands)

    Punt, P.J.; Levasseur, A.; Visser, H.; Wery, J.; Record, E.

    2011-01-01

    For more than a century, filamentous fungi have been used for the production of a wide variety of endogenous enzymes of industrial interest. More recently, with the use of genetic engineering tools developed for these organisms, this use has expanded for the production of nonnative heterologous

  15. In silico product design of pharmaceuticals

    DEFF Research Database (Denmark)

    Boetker, Johan; Raijada, Dhara; Aho, Johanna

    2016-01-01

    Abstract The increasing demand for personalized medicine necessitates the production of easily customizable dosage forms. As the number of possible dosage forms may scale towards infinity, their uniqueness require a versatile production platform and numerical simulation in order to be manufacture...

  16. Tetherin restricts productive HIV-1 cell-to-cell transmission.

    Directory of Open Access Journals (Sweden)

    Nicoletta Casartelli

    2010-06-01

    Full Text Available The IFN-inducible antiviral protein tetherin (or BST-2/CD317/HM1.24 impairs release of mature HIV-1 particles from infected cells. HIV-1 Vpu antagonizes the effect of tetherin. The fate of virions trapped at the cell surface remains poorly understood. Here, we asked whether tetherin impairs HIV cell-to-cell transmission, a major means of viral spread. Tetherin-positive or -negative cells, infected with wild-type or DeltaVpu HIV, were used as donor cells and cocultivated with target lymphocytes. We show that tetherin inhibits productive cell-to-cell transmission of DeltaVpu to targets and impairs that of WT HIV. Tetherin accumulates with Gag at the contact zone between infected and target cells, but does not prevent the formation of virological synapses. In the presence of tetherin, viruses are then mostly transferred to targets as abnormally large patches. These viral aggregates do not efficiently promote infection after transfer, because they accumulate at the surface of target cells and are impaired in their fusion capacities. Tetherin, by imprinting virions in donor cells, is the first example of a surface restriction factor limiting viral cell-to-cell spread.

  17. Marketing and Product Design: A Rocky Love Affair

    Directory of Open Access Journals (Sweden)

    Landwehr Jan R.

    2015-11-01

    Full Text Available The success stories of design-oriented companies like Apple, Audi or Nike have put design on the agenda in many marketing departments. Consumers cannot elude the effect of aesthetics and therefore design is a major factor for business success. Typically consumers choose the product with the best design, all other aspects being equal. Only when the interplay of product characteristics, brand and design is carefully coordinated can successful products be created. This requires an integrated approach to design, one which is applied right at the beginning of the value chain. Product development, marketing and design need to work in close cooperation, communicate well and frequently, and collect feedback from the market. Superior aesthetics are always important but should be a top priority in cases where efficiency-oriented Asian competitors are able to offer functionally similar products at much lower prices.

  18. PROPOSAL OF SPATIAL OPTIMIZATION OF PRODUCTION PROCESS IN PROCESS DESIGNER

    Directory of Open Access Journals (Sweden)

    Peter Malega

    2015-03-01

    Full Text Available This contribution is focused on optimizing the use of space in the production process using software Process Designer. The aim of this contribution is to suggest possible improvements to the existing layout of the selected production process. Production process was analysed in terms of inputs, outputs and course of actions. Nowadays there are many software solutions aimed at optimizing the use of space. One of these software products is the Process Designer, which belongs to the product line Tecnomatix. This software is primarily aimed at production planning. With Process Designer is possible to design the layout of production and subsequently to analyse the production or to change according to the current needs of the company.

  19. Continued SOFC cell and stack technology and improved production methods

    Energy Technology Data Exchange (ETDEWEB)

    Wandel, M.; Brodersen, K.; Phair, J. (and others)

    2009-05-15

    Within this project significant results are obtained on a number of very diverse areas ranging from development of cell production, metallic creep in interconnect to assembling and test of stacks with foot print larger than 500 cm2. Out of 38 milestones 28 have been fulfilled and 10 have been partly fulfilled. This project has focused on three main areas: 1) The continued cell development and optimization of manufacturing processes aiming at production of large foot-print cells, improving cell performance and development environmentally more benign production methods. 2) Stack technology - especially stacks with large foot print and improving the stack design with respect to flow geometry and gas leakages. 3) Development of stack components with emphasis on sealing (for 2G as well as 3G), interconnect (coat, architecture and creep) and test development. Production of cells with a foot print larger than 500 cm2 is very difficult due to the brittleness of the cells and great effort has been put into this topic. Eight cells were successfully produced making it possible to assemble and test a real stack thereby giving valuable results on the prospects of stacks with large foot print. However, the yield rate is very low and a significant development to increase this yield lies ahead. Several lessons were learned on the stack level regarding 'large foot print' stacks. Modelling studies showed that the width of the cell primarily is limited by production and handling of the cell whereas the length (in the flow direction) is limited by e.g. pressure drop and necessary manifolding. The optimal cell size in the flow direction was calculated to be between approx20 cm and < 30 cm. From an economical point of view the production yield is crucial and stacks with large foot print cell area are only feasible if the cell production yield is significantly enhanced. Co-casting has been pursued as a production technique due to the possibilities in large scale production

  20. Product design in the circular economy

    DEFF Research Database (Denmark)

    Atlason, Reynir Smari; Giacalone, Davide; Parajuly, Keshav

    2017-01-01

    . Such strategies may also assist societies in the transition to a more circular economy. However, at the end of a product's useful life, it is uncertain whether the end users will handle the product according to the original intention of the product developers. Situated within this context, this research...... of according to a favorable EoL scenarios, but may not have any prior expectations at point of purchase. Reuse potential emerged as the most attractive EoL scenario, suggesting that users' preferences are largely aligned with the concept of circular economy. Interestingly, women were found to prefer all Eo...

  1. Designing transmitting CMUT cells for airborne applications.

    Science.gov (United States)

    Unlügedik, Aslı; Taşdelen, A; Atalar, Abdullah; Köymen, Hayrettin

    2014-11-01

    We report a new mode of airborne operation for capacitive micromachined ultrasonic transducers (CMUT), in which the plate motion spans the entire gap without collapsing and the transducer is driven by a sinusoidal voltage without a dc bias. We present equivalent-circuit-based design fundamentals for an airborne CMUT cell and verify the design targets using fabricated CMUTs. The performance limits for silicon plates are derived. We experimentally obtain 78.9 dB//20 μPa@1 m source level at 73.7 kHz, with a CMUT cell of radius 2.05 mm driven by 71 V sinusoidal drive voltage at half the frequency. The measured quality factor is 120. We also study and discuss the interaction of the nonlinear transduction force and the nonlinearity of the plate compliance.

  2. Applying axiomatic design methodology in developing modified libertation products

    Directory of Open Access Journals (Sweden)

    Bibiana Margarita Vallejo Díaz

    2004-09-01

    Full Text Available Some conceptual elements regarding the axiomatic design method were applied to a specific case-study regarding developing modified liberation compressed product (CLM-UN, for use in the agricultural sector as pH regulating agent in solil. The study was orientated towards defining functional requeriments, design parameters and process variables for manufacturing the product. Independence and information were evaluated, supporting axiomatic design as an alternative for integral product and process design (as a rational and systemic exercise, facilitating producing products having the quality which future users expect from them.

  3. Product System Design – to Household Massage Design as An Example

    Directory of Open Access Journals (Sweden)

    Wang Huabin

    2016-01-01

    Full Text Available Explain what is the system design and the applications of System design in the product design process. Using The whole idea and systems design methods to analyze the design of household hand massage,Use a chart image to explain that Household hand massage products in the influence of user and the environment, the influence of large system environment for the product. The use of components of the system to anatomy product design. Each system components has a link between and mutual correlation.

  4. Microbial contamination of hematopoietic progenitor cell products.

    Science.gov (United States)

    Namdaroğlu, Sinem; Tekgündüz, Emre; Bozdağ, Sinem Civriz; Durgun, Gamze; Sarıca, Abdurrahman; Demiriz, Itır Şirinoğlu; Koçubaba, Serife; Iskender, Gülşen; Kayıkçı, Omür; Altuntaş, Fevzi

    2013-06-01

    Microbial screening for contamination is a part of hematopoietic progenitor cell (HPC) collection and infusion procedure. We aimed to find out our microbial contamination rates during collection, processing and infusion steps of HPC products. We also evaluated the clinical course of patients who received contaminated HPC products. We retrospectively analyzed microbial contamination records of HPC grafts between 2010 and 2012. HPC products of autologous donors were evaluated for contamination at three steps: at the end of mobilization, following processing with DMSO and just before stem cell infusion. Grafts of allogeneic donors were assessed only before HPC transplantation (HCT). Microbiological analysis of HPC samples were performed with an automated system (BacT/Alert®). During the study period a total of 492 mobilization procedures were performed on 329 (214 autologous and 115 allogeneic) donors. Bacterial contamination has been detected in 103 of 1630 samples (6%). Ninety-seven out of 1162 blood samples (8%) from 265 patients who were treated with HCT were contaminated. Forty-six patients (41 autologous and 5 allogeneic) were transplanted with contaminated HPC products. During HCT 42 patients experienced febrile neutropenic attack and 34 of them had positive blood culture results. In none of these 34 patients the isolated pathogens were the same organisms with those found in the final contaminated stem cell product before stem cell infusion. None of the patients who received contaminated products died because of sepsis within the posttransplant 30days. There was no significant difference between patients who received contaminated and non-contaminated products in terms of the first day of fever, duration of fever, engraftment kinetics and duration of hospitalization. Our results suggest that microbial contamination of HPC products is an issue to be prevented, although it may not have a major impact on the general success of HCT. Copyright © 2013. Published by

  5. Consumers' Kansei Needs Clustering Method for Product Emotional Design Based on Numerical Design Structure Matrix and Genetic Algorithms.

    Science.gov (United States)

    Yang, Yan-Pu; Chen, Deng-Kai; Gu, Rong; Gu, Yu-Feng; Yu, Sui-Huai

    2016-01-01

    Consumers' Kansei needs reflect their perception about a product and always consist of a large number of adjectives. Reducing the dimension complexity of these needs to extract primary words not only enables the target product to be explicitly positioned, but also provides a convenient design basis for designers engaging in design work. Accordingly, this study employs a numerical design structure matrix (NDSM) by parameterizing a conventional DSM and integrating genetic algorithms to find optimum Kansei clusters. A four-point scale method is applied to assign link weights of every two Kansei adjectives as values of cells when constructing an NDSM. Genetic algorithms are used to cluster the Kansei NDSM and find optimum clusters. Furthermore, the process of the proposed method is presented. The details of the proposed approach are illustrated using an example of electronic scooter for Kansei needs clustering. The case study reveals that the proposed method is promising for clustering Kansei needs adjectives in product emotional design.

  6. Overview of the IFMIF test cell design

    International Nuclear Information System (INIS)

    Moeslang, A.; Daum, E.; Jitsukawa, S.; Noda, K.; Viola, R.

    1996-01-01

    The Conceptual Design Activity (CDA) for the International Fusion Materials Irradiation Facility (IFMIF) has entered its second and final year, and an outline design has been developed. Initial evaluations of the potential of this high flux, high intensity D-Li source have shown that the main materials testing needs can be fulfilled. According to these needs, Vertical Test Assemblies will accommodate test modules for the high flux (0.5 liter, 20 dpa/a, 250-1000 C), the medium flux (6 liter, 1-20 dpa/a, 250-1000 C), the low flux (7.5 liter, 0.1-1 dpa/a), and the very low flux (> 100 liter, 0.01-0.1 dpa/a) regions. Detailed test matrices have been defined for the high and medium flux regions, showing that on the basis of small specimen test technologies, a database for an engineering design of an advanced fusion reactor (DEMO) can be established for a variety of structural materials and ceramic breeders. The design concepts for the Test Cell, including test assemblies, remote handling equipment and Hot Cell Facilities with capacity for investigating all irradiation specimens at the IFMIF site are described

  7. PRINCIPLE OF POST-PRODUCTION DESIGN OF HYDRAULIC ACTUATORS

    Directory of Open Access Journals (Sweden)

    A. V. Puzanov

    2015-01-01

    Full Text Available In work the problem of design-technology preparation of production of hydraulic actuators is staticized. The structure and business processes of design and production are analysed. Methods and means of reorganization of project works for the purpose of cutting-down of time of preparation of production are offered. The directions of reorganization of process of design are formulated. The principle of carrying out procedures of design-technology preparation of production of hydraulic actuators with use of ready elements of a production cycle is considered. The scheme of their practical realization at machine-building enterprise is offered. The assessment of growth of efficiency of design-technology preparation of production is given in machine-building enterprise.

  8. On the logistics effects of integrated product and package design

    DEFF Research Database (Denmark)

    Bramklev, Caroline; Hansen, Claus Thorp

    2007-01-01

    obtaining error-free deliveries, saving costs and avoiding quality problems. Thus, the authors of this paper see a challenge in developing the theory and methodology for Integrated Product and Package Design. In this paper we take a first step in this direction. Firstly, we have carried out a broad review......If engineering designers consciously design product and package in an integrated manner, there is a great potential for industrial global enterprises to develop and product mechanical products, such as exhaust extraction systems, car washer machines and fuel handling systems while simultaneously...... of literature, which shows that there is a need for research into Integrated Product and Package Design. Secondly, we have analysed three cases from industrial practice, which show that a conscious and integrated design of product and package has positive logistics effects, whereas neglecting this issue might...

  9. Models and Modelling Tools for Chemical Product and Process Design

    DEFF Research Database (Denmark)

    Gani, Rafiqul

    2016-01-01

    -process design. Illustrative examples highlighting the need for efficient model-based systems will be presented, where the need for predictive models for innovative chemical product-process design will be highlighted. The examples will cover aspects of chemical product-process design where the idea of the grand......The design, development and reliability of a chemical product and the process to manufacture it, need to be consistent with the end-use characteristics of the desired product. One of the common ways to match the desired product-process characteristics is through trial and error based experiments......, which can be expensive and time consuming. An alternative approach is the use of a systematic model-based framework according to an established work-flow in product-process design, replacing some of the time consuming and/or repetitive experimental steps. The advantages of the use of a model...

  10. Group decision support system for customer-driven product design

    Science.gov (United States)

    Lin, Zhihang; Chen, Hang; Chen, Kuen; Che, Ada

    2000-10-01

    This paper describes the work on the development of a group decision support system for customer driven product design. The customer driven is to develop products, which meet all customer requirements in whole life cycle of products. A process model of decision during product primary design is proposed to formulate the structured, semi-structured and unstructured decision problems. The framework for the decision support system is presented that integrated both advances in the group decision making and distributed artificial intelligent. The system consists of the product primary design tool kit and the collaborative platform with multi-agent structure. The collaborative platform of the system and the product primary design tool kit, including the VOC (Voice of Customer) tool, QFD (Quality Function Deployment) tool, the Conceptual design tool, Reliability analysis tool and the cost and profit forecasting tool, are indicated.

  11. On the logistics effects of integrated product and package design

    DEFF Research Database (Denmark)

    Bramklev, Caroline; Hansen, Claus Thorp

    2007-01-01

    If engineering designers consciously design product and package in an integrated manner, there is a great potential for industrial global enterprises to develop and product mechanical products, such as exhaust extraction systems, car washer machines and fuel handling systems while simultaneously...... obtaining error-free deliveries, saving costs and avoiding quality problems. Thus, the authors of this paper see a challenge in developing the theory and methodology for Integrated Product and Package Design. In this paper we take a first step in this direction. Firstly, we have carried out a broad review...... of literature, which shows that there is a need for research into Integrated Product and Package Design. Secondly, we have analysed three cases from industrial practice, which show that a conscious and integrated design of product and package has positive logistics effects, whereas neglecting this issue might...

  12. Clean production and design of products - Complex system and environmental politics of products

    International Nuclear Information System (INIS)

    Sanchez, M.

    1999-01-01

    The orientation of the environmental problem in sustainable terms, especially in the industrialized economies, a new profile in the last years has found. In these economies, the search of a clean production franked by a coherent politics of products and the contribution of the design of products, it have given to the environmental speech a new dimension. With the present text it is sought to respond to the necessity of exploring in Colombia this topic that has a decisive paper inside the instruments of protection of the environment cooperating to improve the quality of life in other latitudes. At the moment the theoretical foundations around the development of products play a important role in the different lines of applied investigation carried out by these countries, especially in the mark of a politics of sustainable development. In spite of the importance of this factor in the industrialized countries where the capacity of integration of a coordinated administration is of great meaning, in Colombia, particularly, the relationship between development and design of products with industry, investigation and political of development and, for our case with a clean production, it is characterized by the scarce insert and attention of this discipline

  13. Designer proton-channel transgenic algae for photobiological hydrogen production

    Science.gov (United States)

    Lee, James Weifu [Knoxville, TN

    2011-04-26

    A designer proton-channel transgenic alga for photobiological hydrogen production that is specifically designed for production of molecular hydrogen (H.sub.2) through photosynthetic water splitting. The designer transgenic alga includes proton-conductive channels that are expressed to produce such uncoupler proteins in an amount sufficient to increase the algal H.sub.2 productivity. In one embodiment the designer proton-channel transgene is a nucleic acid construct (300) including a PCR forward primer (302), an externally inducible promoter (304), a transit targeting sequence (306), a designer proton-channel encoding sequence (308), a transcription and translation terminator (310), and a PCR reverse primer (312). In various embodiments, the designer proton-channel transgenic algae are used with a gas-separation system (500) and a gas-products-separation and utilization system (600) for photobiological H.sub.2 production.

  14. QFood - Optimal design of food products

    DEFF Research Database (Denmark)

    Bech, Anne C.; Engelund, Erling; Juhl, Hans Jørn

    1994-01-01

    and quantitative methods. The QFood-project focuses on the quantitative part and two common methods, multi-dimensional scaling (MDS) and conjoiont analysis are briefly described. 4. Integration between sensory analysis and market analysis on the basis of House of Quality is the central part of the project. House...... of Quality is described with special reference to the development of food products. 5. An MDS-based model for use in the evaluation of an optimal product is developed. The model is based on the profit function from classical micro-economic theory. The imputed price is defined as a function of a Customer...... Satisfaction Index which is inversely proportional to how ""close"" the product is to the consumer's ideal....

  15. New Vistas in Chemical Product and Process Design.

    Science.gov (United States)

    Zhang, Lei; Babi, Deenesh K; Gani, Rafiqul

    2016-06-07

    Design of chemicals-based products is broadly classified into those that are process centered and those that are product centered. In this article, the designs of both classes of products are reviewed from a process systems point of view; developments related to the design of the chemical product, its corresponding process, and its integration are highlighted. Although significant advances have been made in the development of systematic model-based techniques for process design (also for optimization, operation, and control), much work is needed to reach the same level for product design. Timeline diagrams illustrating key contributions in product design, process design, and integrated product-process design are presented. The search for novel, innovative, and sustainable solutions must be matched by consideration of issues related to the multidisciplinary nature of problems, the lack of data needed for model development, solution strategies that incorporate multiscale options, and reliability versus predictive power. The need for an integrated model-experiment-based design approach is discussed together with benefits of employing a systematic computer-aided framework with built-in design templates.

  16. A Systematic Optimization Design Method for Complex Mechatronic Products Design and Development

    Directory of Open Access Journals (Sweden)

    Jie Jiang

    2018-01-01

    Full Text Available Designing a complex mechatronic product involves multiple design variables, objectives, constraints, and evaluation criteria as well as their nonlinearly coupled relationships. The design space can be very big consisting of many functional design parameters, structural design parameters, and behavioral design (or running performances parameters. Given a big design space and inexplicit relations among them, how to design a product optimally in an optimization design process is a challenging research problem. In this paper, we propose a systematic optimization design method based on design space reduction and surrogate modelling techniques. This method firstly identifies key design parameters from a very big design space to reduce the design space, secondly uses the identified key design parameters to establish a system surrogate model based on data-driven modelling principles for optimization design, and thirdly utilizes the multiobjective optimization techniques to achieve an optimal design of a product in the reduced design space. This method has been tested with a high-speed train design. With comparison to others, the research results show that this method is practical and useful for optimally designing complex mechatronic products.

  17. Low power and reliable SRAM memory cell and array design

    CERN Document Server

    Ishibashi, Koichiro

    2011-01-01

    Success in the development of recent advanced semiconductor device technologies is due to the success of SRAM memory cells. This book addresses various issues for designing SRAM memory cells for advanced CMOS technology. To study LSI design, SRAM cell design is the best materials subject because issues about variability, leakage and reliability have to be taken into account for the design.

  18. Explaining the design & styling of future products

    NARCIS (Netherlands)

    Eggink, Wouter; Reinders, Angelina H.M.E.

    2013-01-01

    In many industries technological innovation is the most important driver of competitive success. Within our master curriculum Industrial Design Engineering, we therefore implemented a course that is dedicated to the development of innovative technology. This course, called “Sources of Innovation”,

  19. Production Quality Control Of Microfluidic Chip Designs

    DEFF Research Database (Denmark)

    Calaon, Matteo; Hansen, Hans Nørgaard; Tosello, Guido

    2012-01-01

    process chain, test geometries were designed and produced on the side of the functional features. The so called “Finger Print” of the lithography and molding processes was qualitatively and quantitatively evaluated through scanning electron microscopy and atomic force microscopy respectively. The entire...

  20. Work productivity in rhinitis using cell phones

    DEFF Research Database (Denmark)

    Bousquet, J.; Bewick, Bridgette M; Arnavielhe, S

    2017-01-01

    Allergic rhinitis often impairs social life and performance. The aim of this cross-sectional study was to use cell phone data to assess the impact on work productivity of uncontrolled rhinitis assessed by visual analogue scale (VAS). A mobile phone app (Allergy Diary, Google Play Store and Apple...

  1. the production of mouse embryonic stem cells

    Indian Academy of Sciences (India)

    MADU

    result of the anarchic development of early embryonic cells left behind during development. However, their localization in the genital organs supported an alternative hypothesis: they were the result of an anarchic multiplication of the. Series. What history tells us. VII. Twenty-five years ago: the production of mouse embryonic ...

  2. On the design of product integrated photovoltaic systems

    Energy Technology Data Exchange (ETDEWEB)

    Reich, N.H.

    2010-09-14

    With photovoltaic (PV) systems it is possible to create electricity generation systems for a wide range of purposes, of literally any size (micro watts to giga watts). Solar cells deployed in large scale, grid-connected PV systems may energize millions of electric appliances connected by a utility grid. Solar cells may also be incorporated into single electronic appliances, such as calculators. In this thesis, the focus is primarily upon these Product Integrated PV (PIPV) systems. Employing solar cells to power electronic appliances could have beneficial effects in many aspects. For one, the economic enticement seems attractive, which is due to the large quantities of units involved in present-day product manufacturing. The environment could also benefit, especially when PIPV systems replace products nowadays shipped along with primary (single-use) batteries. Solar PV may also help powering electronic appliances for approximately 1.6 billion people who, as of yet, lack access to utility grids entirely. Finally, also consumer awareness toward PV technology in more general terms can be influenced. Many of the PIPV systems currently commercially available, however, show shortcomings making them non-competitive to their non-PV powered counterparts. In this thesis, the focus is on modeling the energetic performance of PIPV systems, from a physical and technological perspective. The aim is to thereby develop methods and tools and to generate data that may facilitate technical decisions during the design process of PIPV systems. The data, methods and (software) tools to be developed should in particular allow for eased energy balancing calculations. One research question addressed is whether and how the use of Computer Aided Design (CAD) software environments allows for simulating PV power output and PV energy yields, respectively. It is found that the three-dimensional drawing, rendering and ray-tracing features of CAD software may provide a key tool for the simulation

  3. Simulation-based optimization for product and process design

    NARCIS (Netherlands)

    Driessen, L.

    2006-01-01

    The design of products and processes has gradually shifted from a purely physical process towards a process that heavily relies on computer simulations (virtual prototyping). To optimize this virtual design process in terms of speed and final product quality, statistical methods and mathematical

  4. Numerical product design: Springback prediction, compensation and optimization

    NARCIS (Netherlands)

    Meinders, Vincent T.; Burchitz, I.A.; Bonte, M.H.A.; Lingbeek, R.A.

    2007-01-01

    Numerical simulations are being deployed widely for product design. However, the accuracy of the numerical tools is not yet always sufficiently accurate and reliable. This article focuses on the current state and recent developments in different stages of product design: springback prediction,

  5. Design of a safe cylindrical lithium/thionyl chloride cell

    Science.gov (United States)

    Johnson, D. H.; Ayers, A. D.; Zupancic, R. L.; Alberto, V. S.; Bailey, J. C.

    1984-05-01

    Cell design criteria have been established which can result in a safe lithium/thionyl chloride cell. A cell vent, a low area internal anode design, cell balance and composition of the cathode-electrolyte solution have been found to be important factors in the design of a safe cell. In addition to routine testing, both undischarged and discharged cells have been subjected to electrical abuse, environmental abuse and mechanical abuse without disassembly.

  6. Designing future products: what difficulties do designers encounter and how can their creative process be supported?

    Science.gov (United States)

    Bonnardel, Nathalie

    2012-01-01

    To remain competitive, companies must regularly offer new products to consumers. A major challenge for designers is therefore to come up with design solutions and define products that are both new and adapted to future users and usages. Although classic methods and ergonomic recommendations are useful in most run-of-the-mill design contexts, they are of limited benefit when the design situation requires greater creativity. This paper therefore addresses issues related to product design by pursuing a triple objective: (1) highlight the difficulties encountered by designers in imagining and conceiving new products, (2) find out which conditions could help designers come up with creative ideas for innovative products, and (3) suggest methods and tools to support designers' creative process and help them take other stakeholders' needs and expectations into consideration.

  7. Pigment Production Analysis in Human Melanoma Cells.

    Science.gov (United States)

    Hopkin, Amelia Soto; Paterson, Elyse K; Ruiz, Rolando; Ganesan, Anand K

    2016-05-25

    The human epidermal melanocyte is a highly specialized pigmented cell that serves to protect the epidermis from ultraviolet (UV) damage through the production of melanin, or melanogenesis. Misregulation in melanogenesis leading to either hyper- or hypo-pigmentation is found in human diseases such as malasma and vitiligo. Current therapies for these diseases are largely unsuccessful and the need for new therapies is necessary. In order to identify genes and or compounds that can alter melanogenesis, methods are required that can detect changes in pigment production as well as expression of key melanogenesis transcription factors and enzymes. Here we describe methods to detect changes in melanogenesis in a human melanoma cell line, MNT-1, by (1) analyzing pigment production by measuring the absorbance of melanin present by spectrophotometry, (2) analyzing transcript expression of potent regulators of melanogenesis by qunatitative reverse-transcription (RT)PCR and (3) analyzing protein expression of potent regulators of melanogenesis by Western blot (WB).

  8. A Product Line Analysis for Eco-Designed Fashion Products: Evidence from an Outdoor Sportswear Brand

    OpenAIRE

    Luo Wang; Bin Shen

    2017-01-01

    With the increasing awareness of sustainability, eco-design has been an important trend in the fashion industry. Many fashion brands such as Nike, Adidas, and The North Face have developed sustainable fashion by incorporating eco-design elements. Eco-design is an important part of sustainable supply chains. In this paper, we conducted a product line analysis of eco-designed products from a famous outdoor sportswear brand, Patagonia. We collected Patagonia’s 2017 Spring Season product line dat...

  9. Product Data Model for Performance-driven Design

    Science.gov (United States)

    Hu, Guang-Zhong; Xu, Xin-Jian; Xiao, Shou-Ne; Yang, Guang-Wu; Pu, Fan

    2017-09-01

    When designing large-sized complex machinery products, the design focus is always on the overall performance; however, there exist no design theory and method based on performance driven. In view of the deficiency of the existing design theory, according to the performance features of complex mechanical products, the performance indices are introduced into the traditional design theory of "Requirement-Function-Structure" to construct a new five-domain design theory of "Client Requirement-Function-Performance-Structure-Design Parameter". To support design practice based on this new theory, a product data model is established by using performance indices and the mapping relationship between them and the other four domains. When the product data model is applied to high-speed train design and combining the existing research result and relevant standards, the corresponding data model and its structure involving five domains of high-speed trains are established, which can provide technical support for studying the relationships between typical performance indices and design parameters and the fast achievement of a high-speed train scheme design. The five domains provide a reference for the design specification and evaluation criteria of high speed train and a new idea for the train's parameter design.

  10. Design of GMP compliance radiopharmaceutical production facility in MINT

    International Nuclear Information System (INIS)

    Anwar Abd Rahman; Shaharum Ramli; M Rizal Mamat Ibrahim; Rosli Darmawan; Yusof Azuddin Ali; Jusnan Hashim

    2005-01-01

    In 1985, MINT built the only radiopharmaceutical production facility in Malaysia. The facility was designed based on IAEA (International Atomic Energy Agency) standard guidelines which provide radiation safety to the staff and the surrounding environment from radioactive contamination. Since 1999, BPFK (Biro Pengawalan Farmaseutikal Kebangsaan) has used the guidelines from Pharmaceutical Inspection Convention Scheme (PICS) to meet the requirements of the Good Manufacturing Practice (GMP) for Pharmaceutical Products. In the guidelines, the pharmaceutical production facility shall be designed based on clean room environment. In order to design a radiopharmaceutical production facility, it is important to combine the concept of radiation safety and clean room to ensure that both requirements from GMP and IAEA are met. The design requirement is necessary to set up a complete radiopharmaceutical production facility, which is safe, has high production quality and complies with the Malaysian and International standards. (Author)

  11. Adiabatic quantum-flux-parametron cell library adopting minimalist design

    International Nuclear Information System (INIS)

    Takeuchi, Naoki; Yamanashi, Yuki; Yoshikawa, Nobuyuki

    2015-01-01

    We herein build an adiabatic quantum-flux-parametron (AQFP) cell library adopting minimalist design and a symmetric layout. In the proposed minimalist design, every logic cell is designed by arraying four types of building block cells: buffer, NOT, constant, and branch cells. Therefore, minimalist design enables us to effectively build and customize an AQFP cell library. The symmetric layout reduces unwanted parasitic magnetic coupling and ensures a large mutual inductance in an output transformer, which enables very long wiring between logic cells. We design and fabricate several logic circuits using the minimal AQFP cell library so as to test logic cells in the library. Moreover, we experimentally investigate the maximum wiring length between logic cells. Finally, we present an experimental demonstration of an 8-bit carry look-ahead adder designed using the minimal AQFP cell library and demonstrate that the proposed cell library is sufficiently robust to realize large-scale digital circuits

  12. Design models in the development of mechatronic products

    DEFF Research Database (Denmark)

    Habib, Tufail; Brunø, Thomas Ditlev; Nielsen, Kjeld

    2012-01-01

    In view of widespread application of mechatronic systems and the competition to offer customized products at high quality and low cost, there has been considerable attention to introduce new methods and models in this regard. This paper explores design process of mechatronic product development...... with the aid of models. The role of software not only as part of the product but also in the development process. These models contribute to product structuring and evaluation of design process by reducing the complexity and their use for product customization....

  13. Should Mary smell like biscuit? Investigation scents in product design

    NARCIS (Netherlands)

    Ludden, G.D.S.; Schifferstein, H.N.J.

    2009-01-01

    In attempt to influence how a product is experienced, designers can manipulate all aspects of a product, including odor. The effect odor has on a consumer’s experience of the product is still not yet understood. Two experiments were conducted in order to shed more light on the influence odor has on

  14. Design and Development of Synthetic Microbial Platform Cells for Bioenergy

    Directory of Open Access Journals (Sweden)

    Sang Jun eLee

    2013-04-01

    Full Text Available The finite reservation of fossil fuels accelerates the necessity of development of renewable energy sources. Recent advances in synthetic biology encompassing systems biology and metabolic engineering enable us to engineer and/or create tailor made microorganisms to produce alternative biofuels for the future bio-era. For the efficient transformation of biomass to bioenergy, microbial cells need to be designed and engineered to maximize the performance of cellular metabolisms for the production of biofuels during energy flow. Toward this end, two different conceptual approaches have been applied for the development of platform cell factories: forward minimization and reverse engineering. From the context of naturally minimized genomes, non-essential energy-consuming pathways and/or related gene clusters could be progressively deleted to optimize cellular energy status for bioenergy production. Alternatively, incorporation of non-indigenous parts and/or modules including biomass degrading enzymes, carbon uptake transporters, photosynthesis, CO2 fixation, and etc. into chassis microorganisms allows the platform cells to gain novel metabolic functions for bioenergy. This review focuses on the current progress in synthetic biology-aided pathway engineering in microbial cells and discusses its impact on the production of sustainable bioenergy.

  15. Double Image Design in Newspaper Production

    Directory of Open Access Journals (Sweden)

    Mario Barišić

    2010-01-01

    Full Text Available In the high circulation production of daily newspapers, a double image, double information is set under the rules of Infraredesign theory (Pap et al, 2010. The management of visible and near infrared is posted with process colors for color setting configured for conventional newspaper print. The place of imprint that has delimited information in vs (Visible Spectrum and nir (Near InfraRed is called “a print with an infrared effect,” or conditionally “a print with infrared colors.” Daily newspapers, as massive carriers of information made by printing technique, are receiving a new form of presentation: printed image with a built-in invisible image.

  16. Life-cycle analysis of product integrated polymer solar cells

    DEFF Research Database (Denmark)

    Espinosa Martinez, Nieves; García-Valverde, Rafael; Krebs, Frederik C

    2011-01-01

    A life cycle analysis (LCA) on a product integrated polymer solar module is carried out in this study. These assessments are well-known to be useful in developmental stages of a product in order to identify the bottlenecks for the up-scaling in its production phase for several aspects spanning from...... economics through design to functionality. An LCA study was performed to quantify the energy use and greenhouse gas (GHG) emissions from electricity use in the manufacture of a light-weight lamp based on a plastic foil, a lithium-polymer battery, a polymer solar cell, printed circuitry, blocking diode......, switch and a white light emitting semiconductor diode. The polymer solar cell employed in this prototype presents a power conversion efficiency in the range of 2 to 3% yielding energy payback times (EPBT) in the range of 1.3–2 years. Based on this it is worthwhile to undertake a life-cycle study...

  17. Model based process-product design and analysis

    DEFF Research Database (Denmark)

    Gani, Rafiqul

    This paper gives a perspective on modelling and the important role it has within product-process design and analysis. Different modelling issues related to development and application of systematic model-based solution approaches for product-process design is discussed and the need for a hybrid......, forms and complexity, together with their associated parameters. An example of a model-based system for design of chemicals based formulated products is also given....... model-based framework is highlighted. This framework should be able to manage knowledge-data, models, and associated methods and tools integrated with design work-flows and data-flows for specific product-process design problems. In particular, the framework needs to manage models of different types...

  18. Plutonium production in a remote cell

    International Nuclear Information System (INIS)

    Maddux, E.P.; Purcell, J.A.

    1987-01-01

    Production of 239 Pu metal has traditionally been carried out in glovebox enclosures for protection of operating personnel and the environment. With the advent of more stringent federal regulations for protecting personnel, the environment, and security of special nuclear materials, it is proposed that plutonium be processed in a totally remote environment using automated work stations and robotic materials transfer and equipment maintenance. At the Savannah River Plant, the existing plutonium production facility is a batch-type glovebox operation built in the 1950s. A viable alternative to restoration of the existing glovebox operation is to provide a facility that uses remote cell operation

  19. Modular Product Verifications Based on Design for Assembly

    OpenAIRE

    Kenger, Patrik; Bergdahl, Anders; Onori, Mauro

    2005-01-01

    The desire to conquer markets through advanced product design and trendy business strategies are still predominant approaches in industry today. In fact, product development has acquired an ever more central role in the strategic planning of companies, and it has extended its influence to R&D funding levels as well. It is not surprising that many national R&D project frameworks within the EU today are dominated by product development topics, leaving production engineering, robotics, a...

  20. Integrating Emotional Attachment and Sustainability in Electronic Product Design

    Directory of Open Access Journals (Sweden)

    Alex Lobos

    2013-03-01

    Full Text Available Current models for Information and Communication Technology (ICT products encourage frequent product replacement with newer versions that offer only minor incremental improvements. This pattern, named planned obsolescence, diminishes user experience and shortens product lifespan. This paper presents the conceptual basis for a two-part integrated approach to combating planned obsolescence in ICT devices. First, design for emotional attachment, which creates products that users enjoy, value, and use for longer. Second, technological adaptability, which anticipates product upgrades and repairs as new technologies emerge. A model interdisciplinary design course in industrial design and sustainability, also described herein, trains students to apply this approach to create innovative ICT products with smaller environmental footprints.

  1. Designer cell signal processing circuits for biotechnology.

    Science.gov (United States)

    Bradley, Robert W; Wang, Baojun

    2015-12-25

    Microorganisms are able to respond effectively to diverse signals from their environment and internal metabolism owing to their inherent sophisticated information processing capacity. A central aim of synthetic biology is to control and reprogramme the signal processing pathways within living cells so as to realise repurposed, beneficial applications ranging from disease diagnosis and environmental sensing to chemical bioproduction. To date most examples of synthetic biological signal processing have been built based on digital information flow, though analogue computing is being developed to cope with more complex operations and larger sets of variables. Great progress has been made in expanding the categories of characterised biological components that can be used for cellular signal manipulation, thereby allowing synthetic biologists to more rationally programme increasingly complex behaviours into living cells. Here we present a current overview of the components and strategies that exist for designer cell signal processing and decision making, discuss how these have been implemented in prototype systems for therapeutic, environmental, and industrial biotechnological applications, and examine emerging challenges in this promising field. Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.

  2. Design Process Components and Perceived Product Quality : Can a Design Education Program Contribute to better Product Quality?

    NARCIS (Netherlands)

    Kok, B.N.E.

    2016-01-01

    In this PhD research the design process components that can contribute to a better perceived product quality are studied. First the design process components are determined. Secondly the effect of these components on the perceived quality is studied. This research is mainly based on design processes

  3. The power of design product innovation in sustainable energy technologies

    CERN Document Server

    Reinders, Angele H; Brezet, Han

    2012-01-01

    The Power of Design offers an introduction and a practical guide to product innovation, integrating the key topics that are necessary for the design of sustainable and energy-efficient products using sustainable energy technologies. Product innovation in sustainable energy technologies is an interdisciplinary field. In response to its growing importance and the need for an integrated view on the development of solutions, this text addresses the functional principles of various energy technologies next to the latest design processes and innovation methods. From the perspec

  4. Platelet production from induced pluripotent stem cells.

    Science.gov (United States)

    Sugimoto, N; Eto, K

    2017-09-01

    Ex vivo production of human platelets has been pursued as an alternative measure to resolve limitations in the supply and safety of current platelet transfusion products. To this end, induced pluripotent stem cells (iPSCs) are considered an ideal global source, as they are not only pluripotent and self-renewing, but are also available from basically any person, have relatively few ethical issues, and are easy to manipulate. From human iPSCs, megakaryocyte (MK) lines with robust proliferation capacity have been established by the introduction of specified sets of genes. These expandable MKs are also cryopreservable and thus would be suitable as master cells for good manufacturing practice (GMP)-grade production of platelets, assuring availability on demand and safety against blood-borne infections. Meanwhile, developments in bioreactors that physically mimic the in vivo environment and discovery of substances that promote thrombopoiesis have yielded competent platelets with improved efficiency. The derivation of platelets from iPSCs could further resolve transfusion-related alloimmune complications through the manufacturing of autologous products and human leukocyte antigen (HLA)-compatible platelets from stocked homologous HLA-type iPSC libraries or by manipulation of HLAs and human platelet antigens (HPAs). Considering these key advances in the field, HLA-deleted platelets could become a universal product that is manufactured at industrial level to safely fulfill almost all demands. In this review, we provide an overview of the ex vivo production of iPSC-derived platelets toward clinical applications, a production that would revolutionize the blood transfusion system and lead the field of iPSC-based regenerative medicine. © 2017 International Society on Thrombosis and Haemostasis.

  5. SUSTAINABLE PRODUCT DESIGN AND EXAMPLES OF LEATHER MATERIAL RECYCLING

    Directory of Open Access Journals (Sweden)

    GÜRLER KARAMAN Deniz

    2017-05-01

    Full Text Available Many garments made of leather, end up in landfills as waste following the end of its useful life. However, in the flow of production of a leather product, intense energy, chemicals, high volumes of water are consumed. This means that the carbon footprint and environmental loads are high. There are many research activities related to the recycling of textile products, and recycling chains, in this regard famous clothing brands have been organizing grand campaigns. In order to assess the case for leather products that have an important place in the ready-to-wear segment, one should ask the following questions: “How do the big companies and brands in this sector participate in the environmental movement? And importantly, what are the best attempts to recycle leather products? What can be done about the future of leather products recycling and innovative sustainable designs?” when considering sustainable design using recycled leather from end-of-life leather products. In this study, examples of innovative best practices, which were adopted by new brands for recycling and reuse of various types of waste, in order to perform a sustainable product design were presented with the attempt of clarifying aforementioned questions. These new initiatives and practices can develop a novel perspective for academicians and professionals engaged in the field of leather and fashion design, and the concept of sustainable design can be introduced to wider masses.

  6. Aplicability of golden ratio rule in modern product design

    Directory of Open Access Journals (Sweden)

    Darko Avramović

    2013-06-01

    Full Text Available This paper presents research regarding application of golden ratio rule in field of product design. The rule was applied to the design on vacuum cleaner, as it is a common and widely spread product. The aim of this paper is to research possibilities of applications of the rule in modern design. In order to determine consumer's subjective aesthetic judgment of products designed by directly applying golden ratio rule, one vacuum cleaner was designed in that manner. Results for consumer's subjective aesthetic preference were compared to results for other differently designed vacuum cleaner models present in the market today. Factors used were age, gender and level of education of participants (typical consumer. The results showed no statistical influence of age or gender factors. Statistically significant differences were noticed for level of education factor, between participants with high school diploma and university diploma. It was observed that product designed using golden ratio rule received higher marks regarding aesthetics in case of participants with university diploma. It can be concluded that golden ratio rule can be applied in field of product design, equally successful as in the other art fields where it is commonly used, but consumer demographics must be taken in to consideration, especially education level.

  7. Cell-Free Synthesis Meets Antibody Production: A Review

    Directory of Open Access Journals (Sweden)

    Marlitt Stech

    2015-01-01

    Full Text Available Engineered antibodies are key players in therapy, diagnostics and research. In addition to full size immunoglobulin gamma (IgG molecules, smaller formats of recombinant antibodies, such as single-chain variable fragments (scFv and antigen binding fragments (Fab, have emerged as promising alternatives since they possess different advantageous properties. Cell-based production technologies of antibodies and antibody fragments are well-established, allowing researchers to design and manufacture highly specific molecular recognition tools. However, as these technologies are accompanied by the drawbacks of being rather time-consuming and cost-intensive, efficient and powerful cell-free protein synthesis systems have been developed over the last decade as alternatives. So far, prokaryotic cell-free systems have been the focus of interest. Recently, eukaryotic in vitro translation systems have enriched the antibody production pipeline, as these systems are able to mimic the natural pathway of antibody synthesis in eukaryotic cells. This review aims to overview and summarize the advances made in the production of antibodies and antibody fragments in cell-free systems.

  8. Ergonomics and sustainability in the design of everyday use products.

    Science.gov (United States)

    Tosi, Francesca

    2012-01-01

    The relationship between Ergonomics and Design is a key element in the sustainability project, as well as in many other areas of experimental design. In the Design for Sustainability field, Ergonomics is a strategic factor for design culture innovation, providing designers with the necessary knowledge and skills regarding human characteristics and capabilities, as well as user needs and desires during use and interaction with products in work activities and everyday life. Ergonomics is also a strategic innovative factor in design development and manufacturing processes. In fact, ergonomics provides a methodological approach in user-product interaction evaluation processes through the use of participatory design and survey methods, user trials, direct observation, savings and resource conservation, etc.On the other hand, design offers solutions able to interpret user needs and expectations, at the same time suggesting new behaviors and lifestyles.In Design for Sustainability, the ergonomic and user-centered approach contributes greatly to lifestyles and innovative use of products--making it possible to understand and interpret real people needs and expectations in their everyday actions and behavior.New consumption patterns, new awareness of lifestyles, energy source consumption, purchasing methods and consumption style etc. can be supported by design innovation, responding to expressed and unexpressed user needs. With this in mind, the ergonomic approach represents the starting point for design choices and at the same time, a tool for assessing their appropriateness and effectiveness.

  9. The Process of Optimizing Mechanical Sound Quality in Product Design

    DEFF Research Database (Denmark)

    Eriksen, Kaare; Holst, Thomas

    2011-01-01

    The research field concerning optimizing product sound quality is a relatively unexplored area, and may become difficult for designers to operate in. To some degree, sound is a highly subjective parameter, which is normally targeted sound specialists. This paper describes the theoretical...... and practical background for managing a process of optimizing the mechanical sound quality in a product design by using simple tools and workshops systematically. The procedure is illustrated by a case study of a computer navigation tool (computer mouse or mouse). The process is divided into 4 phases, which...... clarify the importance of product sound, defining perceptive demands identified by users, and, finally, how to suggest mechanical principles for modification of an existing sound design. The optimized mechanical sound design is followed by tests on users of the product in its use context. The result...

  10. The Influences of Designing for Services in Product Design Oriented Organisations

    DEFF Research Database (Denmark)

    Akoglu, Canan

    2017-01-01

    of experience working in design consultancies and companies in Sweden and Denmark. Based on the framework of this paper, there are 3 levels of influences as powerful indications for change in product design oriented organisations: re-structuring the approach to design problems, re-structuring the way design...... organisations work internally and collaborate with their clients and re-structuring/re-organising the design organisation itself. The three levels of influence depend on industry needs, demands from client companies, users and how those needs and demands affect product design oriented organisations in addition...... sector in industrialized economies, the shift from manufacturing industries to service industries and the penetration of information and communication technologies especially in daily life have taken attraction to service design and product design oriented organisations cannot stay away from...

  11. Design for usability: practice oriented research for user-centered product design

    NARCIS (Netherlands)

    van Eijk, D.; Hoolhorst, F.W.B.; Kim, F.; Harkema, C.; Dorrestijn, Steven; van Kuijk, J.

    2012-01-01

    The Design for Usability project aims at improving the usability of electronic professional and consumer products by creating new methodology and methods for user-centred product development, which are feasible to apply in practice. The project was focused on 5 key areas: (i) design methodology,

  12. Comparing Personal Characteristic Factors of Imagination between Expert and Novice Designers within Different Product Design Stages

    Science.gov (United States)

    Huang, Yinghsiu; Li, Jianyou

    2015-01-01

    Imagination plays a key role in various domains in helping to create innovative ideas, drawings, poems, movies, products, etc. In product design domain, the personal characteristics of imagination are crucial abilities for conceiving novel ideas during design processes. This study focuses on personal characteristic differences and similarities…

  13. Supporting Production System Design Decisions through Discrete Event Simulation

    OpenAIRE

    Flores-García, Erik

    2017-01-01

    Manufacturing companies are increasingly required to deal with and introduce significant changes in their production systems to gain a competitive advantage. The production system design process is widely considered a means of introducing such changes, and decisions made during design are viewed as critical to its characterization and performance. However, this presents a problem because committing to decisions that involve significant changes implies not only dealing with requirements, produ...

  14. Product Modelling for Building Design: Annotated Bibliography (2nd Edition)

    DEFF Research Database (Denmark)

    Galle, Per

    1999-01-01

    This bibliography concerns research publications from 1976 to 1994-5, on product modelling in computer aided architectural design and computer aided engineering design of buildings and their surroundings. For each item of literature, full bibliographic information is given whenever available...... of literature is offered on machine interpretation of drawings, which may be relevant in the context of information exchange among different product models. Although the bibliography is fairly comprehensive as far as it goes, no completeness of coverage is claimed....

  15. Collaborative Product Design for Tasks Sorting Based on Shortest Delivery

    Directory of Open Access Journals (Sweden)

    Xuedong Liang

    2016-01-01

    Full Text Available The “people’s innovation” can promote development in enterprises in urgent need of innovative product design. Collaborative product design can be a powerful tool for enterprises wishing to improve their market competitiveness and customer satisfaction. To reduce decision costs, improve efficiency, and solve other issues, promoting “people’s innovation” can play a vital role. With this focus, this paper examines products produced through “people’s innovation.” A collaborative design task scheduling problem is presented. The design tasks are sorted based on a minimum delivery cost principle, which is determined using weighted shortest processing time (WSPT rules and the shortest delivery time. The results show that distributed collaborative innovation can result in a reasonable arrangement for collaborative design tasks.

  16. Choosing the right platform for the right product: Sustainable production of chemicals in microbial cell factories

    DEFF Research Database (Denmark)

    Herrgard, Markus

    The Novo Nordisk Foundation Center for Biosustainability (CFB) is a new non-profit research center focused on sustainable production of biochemicals and therapeutic proteins using microbial and mammalian cell factories. The work at CFB is organized around an iterative loop where cell factories...... are taken through four stages: 1) In silico design and data analysis, 2) genome engineering, 3) characterization and high throughput screening, and 4) omics based systems analysis. However, before entering this loop, significant two significant decisions need to be made. First, the choice of what products...... should be made should be made considering biological, technical, economic, and ecological factors. Second, the choice which platform strains will be used to develop production hosts should be made based on characterization of both metabolic and process tolerance traits of strains. I will discuss the role...

  17. The Design and Development of Enhanced Thermal Desorption Products

    Directory of Open Access Journals (Sweden)

    R. Humble

    2005-01-01

    Full Text Available This research study is based on a knowledge-transfer collaboration between The National Centre for Product Design and Development Research (PDR and Markes International Ltd. The aim of the two-year collaboration has been to implement design tools and techniques for the development of enhanced thermal desorption products. Thermal desorption is a highly-specialised technique for the analysis of trace-level volatile organic compounds. This technique allows minute quantities of these compounds to be measured; however, there is an increasing demand from customers for greater sensitivity over a wider range of applications, which means new design methodologies need to be evaluated. The thermal desorption process combines a number of disparate chemical, thermal and mechanical disciplines, and the major design constraints arise from the need to cycle the sample through extremes in temperature. Following the implementation of a comprehensive product design specification, detailed design solutions have been developed using the latest 3D CAD techniques. The impact of the advanced design techniques is assessed in terms of improved product performance and reduced development times, and the wider implications of new product development within small companies are highlighted.  

  18. Design of systems for productivity and well being

    DEFF Research Database (Denmark)

    Edwards, Kasper; Jensen, Per Langaa

    2014-01-01

    It has always been an ambition within the ergonomic profession to ensure that design or redesign of production systems consider both productivity and employee well being, but there are many approaches to how to achieve this. This paper identifies the basic issues to be addressed in light of some...

  19. Practical design and production of optical thin films

    CERN Document Server

    Willey, Ronald R

    2002-01-01

    Fundamentals of Thin Film Optics and the Use of Graphical Methods in Thin Film Design Estimating What Can Be Done Before Designing Fourier Viewpoint of Optical Coatings Typical Equipment for Optical Coating Production Materials and Process Know-How Process Development Monitoring and Control of Thin Film Growth Appendix: Metallic and Semiconductor Material Graphs Author IndexSubject Index

  20. Computational chemical product design problems under property uncertainties

    DEFF Research Database (Denmark)

    Frutiger, Jerome; Cignitti, Stefano; Abildskov, Jens

    2017-01-01

    Three different strategies of how to combine computational chemical product design with Monte Carlo based methods for uncertainty analysis of chemical properties are outlined. One method consists of a computer-aided molecular design (CAMD) solution and a post-processing property uncertainty...

  1. Sustainable Process Design of Biofuels: Bioethanol Production from Cassava rhizome

    DEFF Research Database (Denmark)

    Mangnimit, S.; Malakul, P.; Gani, Rafiqul

    2013-01-01

    .. Also, simultaneously with sustainability analysis, the life cycle impact on environment associated with bioethanol production is performed. Finally, candidate alternative designs are generated and compared with the base case design in terms of LCA, economics, waste, energy usage and enviromental impact...

  2. Design of systems for productivity and well being.

    Science.gov (United States)

    Edwards, Kasper; Jensen, Per Langaa

    2014-01-01

    It has always been an ambition within the ergonomic profession to ensure that design or redesign of production systems consider both productivity and employee well being, but there are many approaches to how to achieve this. This paper identifies the basic issues to be addressed in light of some research activities at DTU, especially by persons responsible for facilitating design processes. Four main issues must be addressed: (1) determining the limits and scope of the system to be designed; (2) identifying stakeholders related to the system and their role in the system design; (3) handling the process' different types of knowledge; and (4) emphasizing that performance management systems, key performance indicators (KPIs), and leadership are also part of the system design and must be given attention. With the examples presented, we argue that knowledge does exist to help system design facilitators address these basic issues. Copyright © 2013. Published by Elsevier Ltd.

  3. Dollar and Senses: Pharmaceutical Product Design is Becoming Vivid

    OpenAIRE

    Schreiber, Stacey L.

    2003-01-01

    Prilosec purple, Viagra blue, and Prozac green and cream: pharmaceutical product design is on the rise, and the future is even brighter. As design features come into view, protection against copying is critical to maintaining the effectiveness of these source-identifying marks. The current state of trademark law allows drug manufacturers to prevent competitors from producing similar medications with the same appearance or identifying design feature. However, while most courts have been increa...

  4. Exploring the influence of design culture on ICT healthcare product and service design for rural China

    NARCIS (Netherlands)

    Jiang, J.; Kandachar, P.V.; Freudenthal, A.

    2010-01-01

    Design culture is usually considered as one of the important aspects that influence designers’ decisions and actions, especially in Design for Base of the Pyramid projects including ICT Healthcare Product Design in Rural China (IHDRC). There are two research questions in this issue: what are the

  5. A Systematic Methodology for Design of Emulsion Based Chemical Products

    DEFF Research Database (Denmark)

    Mattei, Michele; Kontogeorgis, Georgios; Gani, Rafiqul

    2012-01-01

    a hierarchical approach starting with the identification of the needs to be satisfied by the emulsified product and then building up the formulation by adding one-by-one the different classes of chemicals. A structured database together with dedicated property prediction models and evaluation criteria......A systematic methodology for emulsion based chemical product design is presented. The methodology employs a model-based product synthesis/design stage and a modelexperiment based further refinement and/or validation stage. In this paper only the first stage is presented. The methodology employs...

  6. Engineered CHO cells for production of diverse, homogeneous glycoproteins

    DEFF Research Database (Denmark)

    Yang, Zhang; Wang, Shengjun; Halim, Adnan

    2015-01-01

    Production of glycoprotein therapeutics in Chinese hamster ovary (CHO) cells is limited by the cells' generic capacity for N-glycosylation, and production of glycoproteins with desirable homogeneous glycoforms remains a challenge. We conducted a comprehensive knockout screen of glycosyltransferase...

  7. Designing Integrated Product- Service System Solutions in Manufacturing Industries

    DEFF Research Database (Denmark)

    Costa, Nina; Patrício, Lia; Morelli, Nicola

    2015-01-01

    Manufacturing firms are increasingly evolving towards the design of integrated product-service solutions but servitization literature does not provide specific guidance on how to design these integrated solutions. Building upon ProductService System (PSS) and Service Design (SD) approaches......, this paper proposes an integrative method that joins PSS’s systems and network approach with the creative, human-centered, value cocreation approach of SD. The paper also describes the development and application of this method to the creation of integrated solutions for the laboratory industry, highlighting...

  8. Software Product Line for TVDi applications based on design patterns

    Directory of Open Access Journals (Sweden)

    Mirtha Fabiana Miranda

    2016-08-01

    Full Text Available The development of high quality interactive applications for Digital TV (TVDi in industrial scale, requires specific strategies that can be applied in order to develop them. From patterns of interaction design, based on the user, a Software Product Line (SPL for TVDi applications it is proposed to be built. SPL is a form of software development focused on promoting re-using and design patterns of interaction that guarantee the management of products. This project work presents the characteristics of an SPL in order to domain interactive applications for Digital TV, which were validated, mostly based on 32 prototypes, that apply design patterns of interaction based on the user.

  9. Conceiving product ideas in an initial and uncertain design situation

    DEFF Research Database (Denmark)

    Hansen, Claus Thorp; Andreasen, Mogens Myrup

    2006-01-01

    , which carry innovation. Traditional approaches to conceptualisation are no longer sufficient. Too many engineering designers have practiced or cultivated a one-sided product quality focus and too many believe that creativity can compensate for a lack of knowledge and insight into the users’ perception...... of quality and value. Too many engineering designers work stereotypically based on an understanding of the physical product as the core of ideation, and use the conception of a “problem” as the point of departure for the design process. The aim of this research work is to create a multisided, dynamic...

  10. Extend Course for Product Designer in Digital Mobile Era

    Science.gov (United States)

    CHAO, Fang-Lin; LIU, Tzu-Heng; HUANG, Xian-Chun

    2017-12-01

    Product design refers to a system of processes from confirming a product’s specifications to product’s structure. Form, technology, and needs must be considered simultaneously to ensure qualities. In recent years, with the advancement of smartphone technology, many products are connected with apps. Designers cannot exclude themselves from this new wave of the trend. In this article, household hydroponic products design is used as an example, to show the close relationship between digital mobile technology and product design in the contemporary world. Regularly measure the amount of liquid to be added is difficult for a consumer who has no professional experience. To facilitate the introduction of small-scale aquaculture systems into the home, we proposed sensor hardware combined with App software, measured EC and pH value and transmitted to the phone. The app can calculate and display the amount of added and control the amount of inserted through a Bluetooth connection. The physical design needs to take into account the connection between the electronic parts and the circuit board, and interface operation. Thus, not only the model of the product but also the user interface has to be integrated to show the product's quality completely. Besides, authors made reflection upon the necessity for adjustments for interdisciplinary courses under the changing digital mobile era. Also, under the current curriculum structure, possible teaching approach is expressed for extending student’s feasibility.

  11. Affective design identification on the development of batik convection product

    Science.gov (United States)

    Prastawa, H.; Purwaningsih, R.

    2017-11-01

    The affective design is increasingly applied to product development in order to meet the desires and preferences of customers. Batik is a traditional Indonesian culture containing historical and cultural values. The development of batik design is one of the efforts to strengthen the identity and superiority of Indonesia’s creative industries as well as to preserve batik as the cultural heritage of the nation. Batik product designs offered by the manufacturers do not necessarily correspond with the wishes of consumers, especially the affective values involved. Therefore it is necessary to identify consumer perceptions of convection- based batik product in the form of clothing and fabrics, especially the affective value as the consideration for the designer or manufacturer to develop design alternatives to batik convection products. This research aims to obtain information on consumer affective value, to identify the affective value perception differences among X and Y Generation and to classify affective value in the corresponding cluster of the batik products convection. This study uses Kansei engineering to determine the perception of affective design in the form of Kansei word. Cluster Analysis was used to form clusters that classify affective value of the same class. The results showed that there were 16 pairs of Kansei word which was worth as an affective consumer desire, the 3 indicators that had significant differences among X and Y Generation and 4 clusters with different characteristics.

  12. Transforming Multidisciplinary Customer Requirements to Product Design Specifications

    Science.gov (United States)

    Ma, Xiao-Jie; Ding, Guo-Fu; Qin, Sheng-Feng; Li, Rong; Yan, Kai-Yin; Xiao, Shou-Ne; Yang, Guang-Wu

    2017-09-01

    With the increasing of complexity of complex mechatronic products, it is necessary to involve multidisciplinary design teams, thus, the traditional customer requirements modeling for a single discipline team becomes difficult to be applied in a multidisciplinary team and project since team members with various disciplinary backgrounds may have different interpretations of the customers' requirements. A new synthesized multidisciplinary customer requirements modeling method is provided for obtaining and describing the common understanding of customer requirements (CRs) and more importantly transferring them into a detailed and accurate product design specifications (PDS) to interact with different team members effectively. A case study of designing a high speed train verifies the rationality and feasibility of the proposed multidisciplinary requirement modeling method for complex mechatronic product development. This proposed research offersthe instruction to realize the customer-driven personalized customization of complex mechatronic product.

  13. Artefact specification, design, and production as a process of communication

    DEFF Research Database (Denmark)

    Galle, Per

    1999-01-01

    The co-operative process of specifying, designing, and producing an artefact is analysed in terms of communication among the agents involved. By way of introduction, I offer a brief analysis of communication in general, outlining conditions for its success, while avoiding simplistic assumptions...... about communication as literal ‘transmission’ or ‘sharing’ of ideas. It is then explained how the initial artefact specification, the design representation, and ultimately the artefact itself can all be seen as symbolic expressions of ideas that are communicated, and it is shown how the conditions...... for the success of communication carry over to the artefact production process. The purpose of the analysis is to provide a conceptual background for the study of various aspects of artefact production, such as design, or the development of computer-based tools for collaborative design, product modelling...

  14. Property Based Process and Product Synthesis and Design

    DEFF Research Database (Denmark)

    Eden, Mario Richard

    2003-01-01

    in terms of the constitutive (synthesis/design) variables instead of the process variables, thus providing the synthesis/design targets. The second reverse problem (reverse property prediction) solves the constitutive equations to identify unit operations, operating conditions and/or products by matching......This thesis describes the development of a general framework for solving process and product design problems. Targeting the desired performance of the system in a systematic manner relieves the iterative nature of conventional design techniques. Furthermore, conventional component based methods...... are not capable of handling problems, where the process or product objectives are driven by functionalities or properties rather than chemical constituency. The framework is meant to complement existing composition based methods by being able to handle property driven problems. By investigating the different...

  15. Human experience and product usability: principles to assist the design of user-product interactions.

    Science.gov (United States)

    Chamorro-Koc, Marianella; Popovic, Vesna; Emmison, Michael

    2009-07-01

    This paper introduces research that investigates how human experience influences people's understandings of product usability. It describes an experiment that employs visual representation of concepts to elicit participants' ideas of a product's use. Results from the experiment lead to the identification of relationships between human experience, knowledge, and context-of-use--relationships that influence designers' and users' concepts of product usability. These relationships are translated into design principles that inform the design activity with respect to the aspects of experience that trigger people's understanding of a product's use. A design tool (ECEDT) is devised to aid designers in the application of these principles. This tool is then trialled in the context of a design task in order to verify applicability of the findings.

  16. Designed Amino Acid Feed in Improvement of Production and Quality Targets of a Therapeutic Monoclonal Antibody

    Science.gov (United States)

    Torkashvand, Fatemeh; Vaziri, Behrouz; Maleknia, Shayan; Heydari, Amir; Vossoughi, Manouchehr; Davami, Fatemeh; Mahboudi, Fereidoun

    2015-01-01

    Cell culture feeds optimization is a critical step in process development of pharmaceutical recombinant protein production. Amino acids are the basic supplements of mammalian cell culture feeds with known effect on their growth promotion and productivity. In this study, we reported the implementation of the Plackett-Burman (PB) multifactorial design to screen the effects of amino acids on the growth promotion and productivity of a Chinese hamster ovary DG-44 (CHO-DG44) cell line producing bevacizumab. After this screening, the amino acid combinations were optimized by the response surface methodology (RSM) to determine the most effective concentration in feeds. Through this strategy, the final monoclonal antibody (mAb) titre was enhanced by 70%, compared to the control group. For this particular cell line, aspartic acid, glutamic acid, arginine and glycine had the highest positive effects on the final mAb titre. Simultaneously, the impact of the designed amino acid feed on some critical quality attributes of bevacizumab was examined in the group with highest productivity. The product was analysed for N-glycan profiles, charge variant distribution, and low molecular weight forms. The results showed that the target product quality has been improved using this feeding strategy. It was shown how this strategy could significantly diminish the time and number of experiments in identifying the most effective amino acids and related concentrations in target product enhancement. This model could be successfully applied to other components of culture media and feeds. PMID:26480023

  17. Designed Amino Acid Feed in Improvement of Production and Quality Targets of a Therapeutic Monoclonal Antibody.

    Directory of Open Access Journals (Sweden)

    Fatemeh Torkashvand

    Full Text Available Cell culture feeds optimization is a critical step in process development of pharmaceutical recombinant protein production. Amino acids are the basic supplements of mammalian cell culture feeds with known effect on their growth promotion and productivity. In this study, we reported the implementation of the Plackett-Burman (PB multifactorial design to screen the effects of amino acids on the growth promotion and productivity of a Chinese hamster ovary DG-44 (CHO-DG44 cell line producing bevacizumab. After this screening, the amino acid combinations were optimized by the response surface methodology (RSM to determine the most effective concentration in feeds. Through this strategy, the final monoclonal antibody (mAb titre was enhanced by 70%, compared to the control group. For this particular cell line, aspartic acid, glutamic acid, arginine and glycine had the highest positive effects on the final mAb titre. Simultaneously, the impact of the designed amino acid feed on some critical quality attributes of bevacizumab was examined in the group with highest productivity. The product was analysed for N-glycan profiles, charge variant distribution, and low molecular weight forms. The results showed that the target product quality has been improved using this feeding strategy. It was shown how this strategy could significantly diminish the time and number of experiments in identifying the most effective amino acids and related concentrations in target product enhancement. This model could be successfully applied to other components of culture media and feeds.

  18. From product centered design to value centered design: understanding the value-system

    DEFF Research Database (Denmark)

    Randmaa, Merili; Howard, Thomas J.; Otto, T.

    Product design has focused on different parameters through history- design for usability, design for manufacturing, design for assembly etc. Today, as the products get bundled with service, it is important to interconnect product, service and business model design to create synergy effect and off...... value is usually a co-creation process, where customers, suppliers and manufacturers all have their part. This paper describes a paradigm shift towards value-based thinking and proposes a new methodology for understanding and analysing the value system....... more value for the customer for less eford. Value and understanding the value-system needs to be in the focus of business strategy. Value can be created, exchanged and perceived. It can be tangible (physical products, money) or intangible (information, experience, relationships, service). Creating...

  19. Production of soluble Neprilysin by endothelial cells

    Energy Technology Data Exchange (ETDEWEB)

    Kuruppu, Sanjaya, E-mail: Sanjaya.Kuruppu@monash.edu [Department of Biochemistry and Molecular Biology, Building 77, Monash University, Wellington Rd, Clayton, Vic 3800 (Australia); Rajapakse, Niwanthi W. [Department of Physiology, Building 13F, Monash University, Wellington Rd, Clayton, Vic 3800 (Australia); Minond, Dmitriy [Torrey Pines Institute for Molecular Studies, 11350 SW Village Parkway, Port Saint Lucie, FL 34987 (United States); Smith, A. Ian [Department of Biochemistry and Molecular Biology, Building 77, Monash University, Wellington Rd, Clayton, Vic 3800 (Australia)

    2014-04-04

    Highlights: • A soluble full-length form of Neprilysin exists in media of endothelial cells. • Exosomal release is the key mechanism for the production of soluble Neprilysin. • Inhibition of ADAM-17 by specific inhibitors reduce Neprilysin release. • Exosome mediated release of Neprilysin is dependent on ADAM-17 activity. - Abstract: A non-membrane bound form of Neprilysin (NEP) with catalytic activity has the potential to cleave substrates throughout the circulation, thus leading to systemic effects of NEP. We used the endothelial cell line Ea.hy926 to identify the possible role of exosomes and A Disintegrin and Metalloprotease 17 (ADAM-17) in the production of non-membrane bound NEP. Using a bradykinin based quenched fluorescent substrate (40 μM) assay, we determined the activity of recombinant human NEP (rhNEP; 12 ng), and NEP in the media of endothelial cells (10% v/v; after 24 h incubation with cells) to be 9.35 ± 0.70 and 6.54 ± 0.41 μmols of substrate cleaved over 3 h, respectively. The presence of NEP in the media was also confirmed by Western blotting. At present there are no commercially available inhibitors specific for ADAM-17. We therefore synthesised two inhibitors TPI2155-14 and TPI2155-17, specific for ADAM-17 with IC{sub 50} values of 5.36 and 4.32 μM, respectively. Treatment of cells with TPI2155-14 (15 μM) and TPI2155-17 (4.3 μM) resulted in a significant decrease in NEP activity in media (62.37 ± 1.43 and 38.30 ± 4.70, respectively as a % of control; P < 0.0001), implicating a possible role for ADAM-17 in NEP release. However, centrifuging media (100,000g for 1 h at 4 °C) removed all NEP activity from the supernatant indicating the likely role of exosomes in the release of NEP. Our data therefore indicated for the first time that NEP is released from endothelial cells via exosomes, and that this process is dependent on ADAM-17.

  20. Developing a Decision Model of Sustainable Product Design and Development from Product Servicizing in Taiwan

    Science.gov (United States)

    Huang, Yu-Chen; Tu, Jui-Che; Hung, So-Jeng

    2016-01-01

    In response to the global trend of low carbon and the concept of sustainable development, enterprises need to develop R&D for the manufacturing of energy-saving and sustainable products and low carbon products. Therefore, the purpose of this study was to construct a decision model for sustainable product design and development from product…

  1. [Signaling network-based functional cell design].

    Science.gov (United States)

    Ju, Jianqi; Wei, Ping

    2017-03-25

    Cellular signaling networks act as the central processor to deal with environmental signals and regulate cell function, and determine cell fate. Using synthetic biology approach to engineer cell signaling networks is crucial for ultimately constructing man-made "cell machines". Cellular signaling networks can encode sophisticated cell information by processing quantitatively signaling dynamics, which enables multi-dimensional regulation of functional sub-circuits. Here, we first review the research progresses on the signaling coding mechanisms; and then elaborate the methodologies and applications of cells signaling engineering; finally, we envision that signaling-based cell engineering are important for the increasingly-complicated next generation synthetic biology.

  2. Design of Continuous Crystallizers for Production of Active Pharmaceutical Ingredients

    DEFF Research Database (Denmark)

    Capellades Mendez, Gerard; Christensen, Troels V.

    Manufacturing (CPM) could lead to significant reductions in the production costs and an improved consistency of the product quality. As a result, development of such processes has received a significant interest in the past decade. To be able to compete in a patent-driven industry with relatively small annual...... production rates, CPM should be conducted in versatile units that offer short process development times and can be used for production of different compounds. This PhD project deals with the development of novel crystallizer configurations and process design methods oriented to the crystallization of APIs...

  3. Developing engineering design core competences through analysis of industrial products

    DEFF Research Database (Denmark)

    Hansen, Claus Thorp; Lenau, Torben Anker

    2011-01-01

    Most product development work carried out in industrial practice is characterised by being incremental, i.e. the industrial company has had a product in production and on the market for some time, and now time has come to design a new and upgraded variant. This type of redesign project requires...... a course module, in which students develop knowledge, understanding and skills, which will prepare them for being able to participate in and contribute to redesign projects in industrial practice. In the course module Product Analysis and Redesign that has run for 8 years we have developed and refined...

  4. Parametric Design Studies on a Direct Liquid Feed Fuel Cell

    Science.gov (United States)

    Frank, H. A.; Narayanan, S. R.; Nakamura, B.; Surampudi, S.; Halpert, G.

    1995-01-01

    Parametric design studies were carried out on a direct methanol liquid feed fuel cell employing 1 M MeOH fuel, air and oxygen as oxidant in a 2 inch x 2 inch cell employing polymeric electrolyte membranes. Measurements include voltage-current output parameters, methanol crossover rate, and impedance as a function of several design and operational variables. Design variables are described.

  5. Crowdsourcing New Product Design on the Web: An Analysis of Online Designer Platform Service

    Directory of Open Access Journals (Sweden)

    Xin Dai

    2013-01-01

    Full Text Available A designer is a core resource in the fashion industry. Successful designers need to be creative and quick to understand the business and wider environment in which they are operating. The Designer Platform Service (DPS, which combines the mechanism of crowdsourcing and group buying on the web, provides a platform for entrant designers to try their abilities in the real market practice. Freelance designers post design samples or sketches of products on the website of DPS, and consumers may preorder the products (each at a fixed price online based on the design information. Once the number of ordering reaches or passes a certain threshold, that is, the minimum production quantity (MPQ, DPS will arrange for production and delivery according to the orders received. This novel service boosts the growth of entrant designers and links designing works with real markets directly. We are interested in how the price and MPQ decisions are made in DPS, with consideration of the entrant designer's objective, decision sequences, and customer demand structures. We develop Stackelberg games to model and derive the equilibrium solutions under individual scenarios. Our findings suggest feasibility of the DPS business model.

  6. Hemicellulosic ethanol production by immobilized cells of Scheffersomyces stipitis: effect of cell concentration and stirring.

    Science.gov (United States)

    Milessi, Thais S S; Antunes, Felipe A F; Chandel, Anuj K; da Silva, Silvio S

    2015-01-01

    Bioconversion of hemicellulosic hydrolysate into ethanol plays a pivotal role in the overall success of biorefineries. For the efficient fermentative conversion of hemicellulosic hydrolysates into ethanol, the use of immobilized cells system could provide the enhanced ethanol productivities with significant time savings. Here, we investigated the effect of 2 important factors (e.g., cell concentration and stirring) on ethanol production from sugarcane bagasse hydrolysate using the yeast Scheffersomyces stipitis immobilized in calcium alginate matrix. A 2(2) full factorial design of experiment was performed considering the process variables- immobilized cell concentration (3.0, 6.5 and 10.0 g/L) and stirring (100, 200 and 300 rpm). Statistical analysis showed that stirring has the major influence on ethanol production. Maximum ethanol production (8.90 g/l) with ethanol yield (Yp/s) of 0.33 g/g and ethanol productivity (Qp) of 0.185 g/l/h was obtained under the optimized process conditions (10.0 g/L of cells and 100 rpm).

  7. Design and production of the KBS-3 repository

    Energy Technology Data Exchange (ETDEWEB)

    Moren, Lena

    2010-12-15

    The report contains the common basis for a set of Production reports, presenting how the KBS-3 repository is designed, produced and inspected. The set of reports is included in the safety report for the KBS-3 repository and repository facility. The report presents the role of the Production reports within the safety report and their common purposes and objectives. An important part of the report is to present the background and sources to the principles to be applied in the design, the functions of the KBS-3 repository and the barrier functions the engineered barriers and rock. Further, the methodology to substantiate detailed design premises for the engineered barriers, underground openings and other parts of the KBS-3 repository is presented. The report also gives an overview of the KBS-3 system and its facilities and the production lines for the spent fuel, the engineered barriers and underground openings. Finally, an introduction to quality management, safety classification and their application is given

  8. Design and production of the KBS-3 repository

    International Nuclear Information System (INIS)

    Moren, Lena

    2010-12-01

    The report contains the common basis for a set of Production reports, presenting how the KBS-3 repository is designed, produced and inspected. The set of reports is included in the safety report for the KBS-3 repository and repository facility. The report presents the role of the Production reports within the safety report and their common purposes and objectives. An important part of the report is to present the background and sources to the principles to be applied in the design, the functions of the KBS-3 repository and the barrier functions the engineered barriers and rock. Further, the methodology to substantiate detailed design premises for the engineered barriers, underground openings and other parts of the KBS-3 repository is presented. The report also gives an overview of the KBS-3 system and its facilities and the production lines for the spent fuel, the engineered barriers and underground openings. Finally, an introduction to quality management, safety classification and their application is given

  9. DESIGN CONSIDERATIONS UPON PRODUCT END-OF-LIFE OPTIONS

    Directory of Open Access Journals (Sweden)

    BARSAN Lucian

    2016-11-01

    Full Text Available The paper presents some considerations about the necessity of evaluating the environmental impact of a product during its entire life. The present situation (economic, social and ecologic imposes solutions to reduce this impact as a result of an analysis performed during all stages of the life cycle. This paper focuses on design solutions with consequences in the last stage, the end-of-life. Reusing products, with, or without remanufacturing and recycling the materials from products that cannot be reused represent some options analysed in this paper. The end-of-life options should be known even from the beginning of the design process and should be included as design objectives or, at least as constrictions. Considering them as human needs would naturally include them in the requirements list.

  10. Design and Response of a Structural Multifunctional Fuel Cell

    National Research Council Canada - National Science Library

    South, Joseph; Baechle, Daniel; Hilton, Corydon; DeSchepper, Daniel; Wetzel, Eric

    2008-01-01

    .... In this study, structural fuel cells are proposed and evaluated. A structural multifunctional fuel cell system is designed so that material elements participating in power and energy processes are also carrying significant structural loads...

  11. The design of Eco Board Games as an umbrella approach to sustainable product design education

    DEFF Research Database (Denmark)

    Boks, Casper; McAloone, Tim C.

    2009-01-01

    The multidisciplinarity and quickly broadening scope of sustainable product design education provide incentives for experimentation with different pedagogical techniques. One of these, involving the development of eco board games, has been used at both the Technical University Denmark...

  12. Implications of intelligent, integrated microsystems for product design and development

    International Nuclear Information System (INIS)

    MYERS, DAVID R.; MCWHORTER, PAUL J.

    2000-01-01

    Intelligent, integrated microsystems combine some or all of the functions of sensing, processing information, actuation, and communication within a single integrated package, and preferably upon a single silicon chip. As the elements of these highly integrated solutions interact strongly with each other, the microsystem can be neither designed nor fabricated piecemeal, in contrast to the more familiar assembled products. Driven by technological imperatives, microsystems will best be developed by multi-disciplinary teams, most likely within the flatter, less hierarchical organizations. Standardization of design and process tools around a single, dominant technology will expedite economically viable operation under a common production infrastructure. The production base for intelligent, integrated microsystems has elements in common with the mathematical theory of chaos. Similar to chaos theory, the development of microsystems technology will be strongly dependent on, and optimized to, the initial product requirements that will drive standardization--thereby further rewarding early entrants to integrated microsystem technology

  13. The lean product design and development journey a practical view

    CERN Document Server

    Pessôa, Marcus Vinicius Pereira

    2017-01-01

    This book presents a series of high performance product design (PD) and development best practices that can create or improve product development organization. In contrast to other books that focus only on Toyota or other individual companies applying lean IPD, this book explains the lean philosophy more broadly and includes discussions of systems engineering, design for X (DFX), agile development, integrated product development, and project management. The “Lean Journey” proposed here takes a value-centric approach, where the lean principles are applied to PD to allow the tools and methods selected to emerge from observation of the individual characteristics of each enterprise. This means that understanding lean product development (LPD) is not about knowing which tools are available but knowing how to apply the philosophy. The book comes with an accompanying manual with problems and solutions available on Springer Extras.

  14. Co-Design in co-production processes:

    DEFF Research Database (Denmark)

    Seravalli, Anna; Agger Eriksen, Mette; Hillgren, Per-Anders

    2017-01-01

    The public sector, increasingly acknowledging a need for change but strongly influenced by market logics, is experimenting with new forms of co-production of public services based on collaborations between public providers, citizens and societal actors. At the same time, Co-design researchers......, are using approaches of infrastructuring and commoning to navigate questions of participation and collaboration in co-production. By discussing the case of ReTuren, a co-produced service for waste handling and prevention, this article presents how infrastructuring and commoning can offer guidance to civil...... servants engaging in co-production. In the case, civil servants on an operational level and an ‘embedded’ Co-Design researcher worked side-by-side in the co-production of the service, jointly articulating and appropriating approaches of infrastructuring and commoning. The case reveals that the joint...

  15. Effects of blood products on inflammatory response in endothelial cells in vitro.

    Directory of Open Access Journals (Sweden)

    Martin Urner

    Full Text Available BACKGROUND: Transfusing blood products may induce inflammatory reactions within the vascular compartment potentially leading to a systemic inflammatory response. Experiments were designed to assess the inflammatory potential of different blood products in an endothelial cell-based in vitro model and to compare baseline levels of potentially activating substances in transfusion products. METHODS: The inflammatory response from pre-activated (endotoxin-stimulated and non-activated endothelial cells as well as neutrophil endothelial transmigration in response to packed red blood cells (PRBC, platelet concentrates (PC and fresh frozen plasma (FFP was determined. Baseline inflammatory mediator and lipid concentrations in blood products were evaluated. RESULTS: Following incubation with all blood products, an increased inflammatory mediator release from endothelial cells was observed. Platelet concentrates, and to a lesser extent also FFP, caused the most pronounced response, which was accentuated in already pre-stimulated endothelial cells. Inflammatory response of endothelial cells as well as blood product-induced migration of neutrophils through the endothelium was in good agreement with the lipid content of the according blood product. CONCLUSION: Within the group of different blood transfusion products both PC and FFP have a high inflammatory potential with regard to activation of endothelial cells. Inflammation upon blood product exposure is strongly accentuated when endothelial cells are pre-injured. High lipid contents in the respective blood products goes along with an accentuated inflammatory reaction from endothelial cells.

  16. Verifying Architectural Design Rules of the Flight Software Product Line

    Science.gov (United States)

    Ganesan, Dharmalingam; Lindvall, Mikael; Ackermann, Chris; McComas, David; Bartholomew, Maureen

    2009-01-01

    This paper presents experiences of verifying architectural design rules of the NASA Core Flight Software (CFS) product line implementation. The goal of the verification is to check whether the implementation is consistent with the CFS architectural rules derived from the developer's guide. The results indicate that consistency checking helps a) identifying architecturally significant deviations that were eluded during code reviews, b) clarifying the design rules to the team, and c) assessing the overall implementation quality. Furthermore, it helps connecting business goals to architectural principles, and to the implementation. This paper is the first step in the definition of a method for analyzing and evaluating product line implementations from an architecture-centric perspective.

  17. Pharmaceutical product development: A quality by design approach.

    Science.gov (United States)

    Pramod, Kannissery; Tahir, M Abu; Charoo, Naseem A; Ansari, Shahid H; Ali, Javed

    2016-01-01

    The application of quality by design (QbD) in pharmaceutical product development is now a thrust area for the regulatory authorities and the pharmaceutical industry. International Conference on Harmonization and United States Food and Drug Administration (USFDA) emphasized the principles and applications of QbD in pharmaceutical development in their guidance for the industry. QbD attributes are addressed in question-based review, developed by USFDA for chemistry, manufacturing, and controls section of abbreviated new drug applications. QbD principles, when implemented, lead to a successful product development, subsequent prompt regulatory approval, reduce exhaustive validation burden, and significantly reduce post-approval changes. The key elements of QbD viz., target product quality profile, critical quality attributes, risk assessments, design space, control strategy, product lifecycle management, and continual improvement are discussed to understand the performance of dosage forms within design space. Design of experiments, risk assessment tools, and process analytical technology are also discussed for their role in QbD. This review underlines the importance of QbD in inculcating science-based approach in pharmaceutical product development.

  18. Design, production and initial state of the canister

    International Nuclear Information System (INIS)

    Cederqvist, Lars; Johansson, Magnus; Leskinen, Nina; Ronneteg, Ulf

    2010-12-01

    The report is included in a set of Production reports, presenting how the KBS-3 repository is designed, produced and inspected. The set of reports is included in the safety report for the KBS-3 repository and repository facility.The report provides input on the initial state of the canisters to the assessment of the long-term safety, SR-Site. The initial state refers to the properties of the engineered barriers once they have been finally placed in the KBS-3 repository and will not be further handled within the repository facility. In addition, the report provides input to the operational safety report, SR-Operation, on how the canisters shall be handled and disposed. The report presents the design premises and reference design of the canister and verifies the conformity of the reference design to the design premises. The production methods and the ability to produce canisters according to the reference design are described. Finally, the initial state of the canisters and their conformity to the reference design and design premises are presented

  19. Design, production and initial state of the canister

    Energy Technology Data Exchange (ETDEWEB)

    Cederqvist, Lars; Johansson, Magnus; Leskinen, Nina; Ronneteg, Ulf

    2010-12-15

    The report is included in a set of Production reports, presenting how the KBS-3 repository is designed, produced and inspected. The set of reports is included in the safety report for the KBS-3 repository and repository facility.The report provides input on the initial state of the canisters to the assessment of the long-term safety, SR-Site. The initial state refers to the properties of the engineered barriers once they have been finally placed in the KBS-3 repository and will not be further handled within the repository facility. In addition, the report provides input to the operational safety report, SR-Operation, on how the canisters shall be handled and disposed. The report presents the design premises and reference design of the canister and verifies the conformity of the reference design to the design premises. The production methods and the ability to produce canisters according to the reference design are described. Finally, the initial state of the canisters and their conformity to the reference design and design premises are presented

  20. Product and service design for patient centered diabetes care

    Directory of Open Access Journals (Sweden)

    Soumitri Varadarajan

    2009-11-01

    Full Text Available Design plays a marginal part in the discourse of diabetes care, mainly in visualizing the form and packaging of medical technologies. The authors however have a practice that advocates that design orientated solutions can add much needed dimensions to problems that havetraditionally been the exclusive preserve of expert discourses. This position has for long been a validated and largely accepted approach in design’s engagement withissues in sustainability and development studies. A similar approach in the area of medicine has been constructed bythe authors and marks out a position of advocacy where the designer takes on agency to intervene on behalf of the user community. This position contains a healthy critique of thetraditional approach of product design for manufacture while simultaneously amplifying a desire to intervene and make a substantial improvement in the quality of life ofpeople with diabetes. This article first opens out contemporary diabetes care as a contested domain and then goes on to sketch out the key aspects of a design practice focussed upon delivering positive health outcomes in diabetes care. The specific context of discussion for this article is the practice of teaching in design studios wherestudents of design listen to the voices of people with diabetes and visualize ways for design to provide products and service solutions that transform the lived experiences of people with diabetes.

  1. Interactive augmented reality system for product design review

    Science.gov (United States)

    Caruso, Giandomenico; Re, Guido Maria

    2010-01-01

    The product development process, of industrial products, includes a phase dedicated to the design review that is a crucial phase where various experts cooperate in selecting the optimal product shape. Although computer graphics allows us to create very realistic virtual representations of the products, it is not uncommon that designers decide to build physical mock-ups of their newly conceived products because they need to physically interact with the prototype and also to evaluate the product within a plurality of real contexts. This paper describes the hardware and software development of our Augmented Reality design review system that allows to overcome some issues related to the 3D visualization and to the interaction with the virtual objects. Our system is composed by a Video See Through Head Mounted Display, which allows to improve the 3D visualization by controlling the convergence of the video cameras automatically, and a wireless control system, which allows us to create some metaphors to interact with the virtual objects. During the development of the system, in order to define and tune the algorithms, we have performed some testing sessions. Then, we have performed further tests in order to verify the effectiveness of the system and to collect additional data and comments about usability and ergonomic aspects.

  2. Electricity production from microbial fuel cell by using yeast

    International Nuclear Information System (INIS)

    Vorasingha, A.; Souvakon, C.; Boonchom, K.

    2006-01-01

    The continuous search for methods to generate electricity from renewable sources such as water, solar energy, wind, nuclear or chemicals was discussed with particular focus on attaining the full power of the microbial fuel cell (MFC). Under ideal environmental conditions, the only byproducts of a biofuel cell would be water and carbon dioxide (CO 2 ). The production of energy from renewables such as biomass is important for sustainable development and reducing global emissions of CO 2 . Hydrogen can also be an important component of an energy infrastructure that reduces CO 2 emissions if the hydrogen is produced from renewable sources and used in fuel cells. Hydrogen gas can be biologically produced at high concentration from the fermentation of high sugar substrates such as glucose and sucrose. Some of the issues of MFC design were addressed, including the use of cheap substrates to derive microbial electricity. In the MFC, yeast donates electrons to a chemical electron mediator, which in turn transfers the electrons to an electrode, producing electricity. Experimental results showed that glucose yielded the highest peak voltage, but a semi-processed sugar and molasses were similar to glucose in the electricity production pattern. It was noted that this technology is only at the research stages, and more research is needed before household microbial fuel cells can be made available for producing power for prolonged periods of time. Future research efforts will focus on increasing the efficiency, finding alternatives to hazardous electron mediators and finding new microbes. 12 refs., 6 figs

  3. A Product Line Analysis for Eco-Designed Fashion Products: Evidence from an Outdoor Sportswear Brand

    Directory of Open Access Journals (Sweden)

    Luo Wang

    2017-07-01

    Full Text Available With the increasing awareness of sustainability, eco-design has been an important trend in the fashion industry. Many fashion brands such as Nike, Adidas, and The North Face have developed sustainable fashion by incorporating eco-design elements. Eco-design is an important part of sustainable supply chains. In this paper, we conducted a product line analysis of eco-designed products from a famous outdoor sportswear brand, Patagonia. We collected Patagonia’s 2017 Spring Season product line data and analyzed the data through descriptive analysis, factor analysis and correlation analysis. We found that Patagonia mainly uses organic, recycled, and traceable materials in their eco-product line development. We identified that the usage of eco-materials may significantly affect the number of color choices and product weight as well as gender difference, pattern design, product fit and online reviewers’ opinions at Patagonia. We argued that Patagonia should focus on functionality more than aesthetics in eco-design. We discussed how sustainable fashion firms should manage eco-design in the supply chain.

  4. An Exploration of Smart Product-Service System Design : Guidelines and Insights for Design Management

    NARCIS (Netherlands)

    Valencia Cardona, A.M.

    2017-01-01

    This thesis reports on the findings of a research project funded by the Dutch Ministry of Education, Culture and Science. The thesis investigates the design of Smart Product-Service Systems (Smart PSSs), defined as the integration of smart, connected products and e-services, presented to consumers

  5. Effect of design variations on AMTEC cell efficiency, and of operating parameters on performance OSC cell design

    International Nuclear Information System (INIS)

    Schock, A.; Noravian, H.; Kumar, V.; Or, C.

    1997-01-01

    A companion paper presented here described an OSC-derived methodology for the coupled thermal, fluid flow, and electrical analyses of AMTEC cells with multiple Beta Alumina Solid Electrolyte (BASE) tubes, and illustrated its application to a specific cell design. The present paper describes parametric results obtained by applying the same procedure to 39 variations of that cell design to determine the effect of 24 individual design variables on cell performance. For each of the 24 design variables, OSC performed detailed analyses of two designs that were identical except for that variable. This served to isolate the effect of that variable on the cell's power, efficiency, and critical temperatures. The paper presents the results of those analyses, and explains the reasons for the design decisions made by OSC. Subsequently, it describes the results of parametric analyses to determine the effect of temperature margin, input thermal power, and output voltage on the cell's critical temperatures and performance

  6. Sustainable Product Design, Engineering and Management Education for Industrial Design Engineering

    NARCIS (Netherlands)

    Boks, C.; Diehl, J.C.; Wever, R.

    2006-01-01

    Developments in the field of sustainable product design are manifold, which means that education in this field is rapidly evolving as well. In this paper, the continuously evolving portfolio of courses offered at Delft University of Technology’s Industrial Design Engineering faculty is

  7. Design and industrial production of frequency standards in the USSR

    Science.gov (United States)

    Demidov, Nikolai A.; Uljanov, Adolph A.

    1990-01-01

    Some aspects of research development and production of quantum frequency standards, carried out in QUARTZ Research and Production Association (RPA), Gorky, U.S.S.R., were investigated for the last 25 to 30 years. During this period a number of rubidium and hydrogen frequency standards, based on the active maser, were developed and put into production. The first industrial model of a passive hydrogen maser was designed in the last years. Besides frequency standards for a wide application range, RPA QUARTZ investigates metrological frequency standards--cesium standards with cavity length 1.9 m and hydrogen masers with a flexible storage bulb.

  8. Applying Human-Centered Design Methods to Scientific Communication Products

    Science.gov (United States)

    Burkett, E. R.; Jayanty, N. K.; DeGroot, R. M.

    2016-12-01

    Knowing your users is a critical part of developing anything to be used or experienced by a human being. User interviews, journey maps, and personas are all techniques commonly employed in human-centered design practices because they have proven effective for informing the design of products and services that meet the needs of users. Many non-designers are unaware of the usefulness of personas and journey maps. Scientists who are interested in developing more effective products and communication can adopt and employ user-centered design approaches to better reach intended audiences. Journey mapping is a qualitative data-collection method that captures the story of a user's experience over time as related to the situation or product that requires development or improvement. Journey maps help define user expectations, where they are coming from, what they want to achieve, what questions they have, their challenges, and the gaps and opportunities that can be addressed by designing for them. A persona is a tool used to describe the goals and behavioral patterns of a subset of potential users or customers. The persona is a qualitative data model that takes the form of a character profile, built upon data about the behaviors and needs of multiple users. Gathering data directly from users avoids the risk of basing models on assumptions, which are often limited by misconceptions or gaps in understanding. Journey maps and user interviews together provide the data necessary to build the composite character that is the persona. Because a persona models the behaviors and needs of the target audience, it can then be used to make informed product design decisions. We share the methods and advantages of developing and using personas and journey maps to create more effective science communication products.

  9. The process defines the product: what really matters in biosimilar design and production?

    Science.gov (United States)

    Vulto, Arnold G; Jaquez, Orlando A

    2017-08-01

    Biologic drugs are highly complex molecules produced by living cells through a multistep manufacturing process. The key characteristics of these molecules, known as critical quality attributes (CQAs), can vary based on post-translational modifications that occur in the cellular environment or during the manufacturing process. The extent of the variation in each of the CQAs must be characterized for the originator molecule and systematically matched as closely as possible by the biosimilar developer to ensure bio-similarity. The close matching of the originator fingerprint is the foundation of the biosimilarity exercise, as the analytical tools designed to measure differences at the molecular level are far more sensitive and specific than tools available to physicians during clinical trials. Biosimilar development, therefore, has a greater focus on preclinical attributes compared with the development of an original biological agent. As changes in CQAs can occur at different stages of the manufacturing process, even small modifications to the process can alter biosimilar attributes beyond the point of similarity and impact clinical effectiveness and safety. The manufacturer's ability to provide consistent production and quality control will greatly influence the acceptance of biosimilars. To this end, preventing drift from the required specifications over time and avoiding the various implications brought by product shortage will enhance biosimilar integration into daily practice. As most prescribers are not familiar with this new drug development paradigm, educational programmes will be needed so that prescribers see biosimilars as fully equivalent, efficacious and safe medicines when compared with originator products. © The Author 2017. Published by Oxford University Press on behalf of the British Society for Rheumatology.

  10. EXPERIMENTAL RESEARCH REGARDING LEATHER APPLICATIONS IN PRODUCT DESIGN

    Directory of Open Access Journals (Sweden)

    PRALEA Jeni

    2015-05-01

    Full Text Available This paper presents the role and importance of experimental research in design activity. The designer, as a researcher and a project manager, proposes to establish a relationship between functional-aesthetic-constructive-technological-economic,based on the aesthetic possibilities of the materials used for the experiments. With the aim to identify areas for the application of leather waste resulted from the production process, the paper presents experiments conducted with this material in combination with wood, by using different techniques that lead to different aesthetic effects. Identifying the areas to use and creating products from leather and/or wood waste, is based on the properties of these materials. Leather, the subject of these experiments, has the advantage that it can be used on both sides. Tactile differences of the two sides of this material has both aesthetical and functional advantages, which makes it suitable for applications on products that meet the requirements of "design for all". With differentiated tactile characteristics, in combination with other materials, for these experiments wood, easily "read touch" products can be generated to help people with certain disabilities. Thus, experiments presented in this paper allows the establishment of aesthetic schemes applicable to products that are friendly both with the environment (based on the reuse of wood and leather waste and with the users (can be used as applications, accessories and concepts of products for people with certain disabilities. The designer’s choices or decisions can be based on the results of this experiment. The experiment enables the designer to develop creative, innovative and environmentally friendly products.

  11. Design, production and initial state of the buffer

    International Nuclear Information System (INIS)

    Boerjesson, Lennart; Gunnarsson, David; Johannesson, Lars-Erik; Jonsson, Esther

    2010-12-01

    The report is included in a set of Production reports, presenting how the KBS-3 repository is designed, produced and inspected. The set of reports is included in the safety report for the KBS-3 repository and repository facility. The report provides input on the initial state of the buffer for the assessment of the long-term safety, SR-Site. The initial state refers to the properties of the engineered barriers once they have been finally placed in the KBS-3 repository and will not be further handled within the repository facility. In addition, the report provides input to the operational safety report, SR-Operation, on how the buffer shall be handled and installed. The report presents the design premises and reference design of the buffer and verifies the conformity of the reference design to the design premises. It also describes the production of the buffer, from excavation and delivery of buffer material to installation in the deposition hole. Finally, the initial state of the buffer and its conformity to the reference design and design premises is presented

  12. Design, production and initial state of the buffer

    Energy Technology Data Exchange (ETDEWEB)

    Boerjesson, Lennart; Gunnarsson, David; Johannesson, Lars-Erik; Jonsson, Esther

    2010-12-15

    The report is included in a set of Production reports, presenting how the KBS-3 repository is designed, produced and inspected. The set of reports is included in the safety report for the KBS-3 repository and repository facility. The report provides input on the initial state of the buffer for the assessment of the long-term safety, SR-Site. The initial state refers to the properties of the engineered barriers once they have been finally placed in the KBS-3 repository and will not be further handled within the repository facility. In addition, the report provides input to the operational safety report, SR-Operation, on how the buffer shall be handled and installed. The report presents the design premises and reference design of the buffer and verifies the conformity of the reference design to the design premises. It also describes the production of the buffer, from excavation and delivery of buffer material to installation in the deposition hole. Finally, the initial state of the buffer and its conformity to the reference design and design premises is presented

  13. Design, fabrication, test, qualification, and price analysis of third generation design solar cell modules

    Science.gov (United States)

    1981-10-01

    The fabrication of solar cell modules is detailed with emphasis upon laminating and interconnecting the panels that hold the simicrystalline silicon cells. Design problems and enviromental tests are described as well as performance characteristics.

  14. Application of Absorption Modeling in Rational Design of Drug Product Under Quality-by-Design Paradigm.

    Science.gov (United States)

    Kesisoglou, Filippos; Mitra, Amitava

    2015-09-01

    Physiologically based absorption models can be an important tool in understanding product performance and hence implementation of Quality by Design (QbD) in drug product development. In this report, we show several case studies to demonstrate the potential application of absorption modeling in rational design of drug product under the QbD paradigm. The examples include application of absorption modeling—(1) prior to first-in-human studies to guide development of a formulation with minimal sensitivity to higher gastric pH and hence reduced interaction when co-administered with PPIs and/or H2RAs, (2) design of a controlled release formulation with optimal release rate to meet trough plasma concentrations and enable QD dosing, (3) understanding the impact of API particle size distribution on tablet bioavailability and guide formulation design in late-stage development, (4) assess impact of API phase change on product performance to guide specification setting, and (5) investigate the effect of dissolution rate changes on formulation bioperformance and enable appropriate specification setting. These case studies are meant to highlight the utility of physiologically based absorption modeling in gaining a thorough understanding of the product performance and the critical factors impacting performance to drive design of a robust drug product that would deliver the optimal benefit to the patients.

  15. Design of Sustainable Blended Products using an Integrated Methodology

    DEFF Research Database (Denmark)

    Yunus, Nor Alafiza Binti; Gernaey, Krist; Woodley, John

    2013-01-01

    to be used as building blocks in the mixture design, and then to propose the blend formulations that fulfill the desired product attributes. The result is a set of blends that match the constraints, the compositions, values of the target properties and information about their miscibility. The methodology has...

  16. Product design and business model strategies for a circular economy

    NARCIS (Netherlands)

    Bocken, N.M.P.; de Pauw, I.C.; Bakker, C.A.; van der Grinten, B.

    The transition within business from a linear to a circular economy brings with it a range of practical challenges for companies. The following question is addressed: What are the product design and business model strategies for
    companies that want to move to a circular economy model? This paper

  17. Stochastic approaches for product recovery network design: a case study

    NARCIS (Netherlands)

    O.L. Listes (Ovidiu); R. Dekker (Rommert)

    2001-01-01

    textabstractIncreased uncertainty is one of the characteristics of product recovery networks. In particular the strategic design of their logistic infrastructure has to take uncertain information into account. In this paper we present stochastic programming based approaches by which a deterministic

  18. Material properties and processing in chemical product design

    NARCIS (Netherlands)

    Picchioni, F; Broekhuis, A.A.

    2012-01-01

    This paper reviews developments in the first decade since the introduction of chemical product design and engineering as a new disciplinary element within the chemical engineering sciences. This opinion focuses on the differences between processes needed for the manufacture of specified chemicals

  19. Multi-objective evolutionary optimisation for product design and manufacturing

    CERN Document Server

    2011-01-01

    Presents state-of-the-art research in the area of multi-objective evolutionary optimisation for integrated product design and manufacturing Provides a comprehensive review of the literature Gives in-depth descriptions of recently developed innovative and novel methodologies, algorithms and systems in the area of modelling, simulation and optimisation

  20. Design and Production of Trophies for Selected Sports and Games ...

    African Journals Online (AJOL)

    Design and production of trophies for games and sports particularly in a form of metal art has been in existence for so many years. Presently they are practiced with much improvement and modern technology as compared to the ancient times where leaves, horns and other objects were used as trophies to award warriors ...

  1. Reducing ongoing product design decision-making bias

    NARCIS (Netherlands)

    Antioco, M.; Moenaert, R.K.; Lindgreen, A.

    2008-01-01

    The objective of this exploratory study is to add to our understanding of ongoing product design decision-making to reduce eventual decision-making bias. Six research questions are formulated with the aim to establish if and how functional membership and informal patterns of communication within an

  2. Exploring the use of consumer collages in product design

    NARCIS (Netherlands)

    Costa, A.I.A.; Schoolmeester, D.; Dekker, M.; Jongen, W.M.F.

    2003-01-01

    This paper presents the development, application and evaluation of a method for need identification in food product design, which combines the use of collage techniques and focus groups. Recent findings in neurobiology and psychology have considerable relevance for methodological improvement in

  3. 47 CFR 6.7 - Product design, development, and evaluation.

    Science.gov (United States)

    2010-10-01

    ... 47 Telecommunication 1 2010-10-01 2010-10-01 false Product design, development, and evaluation. 6.7 Section 6.7 Telecommunication FEDERAL COMMUNICATIONS COMMISSION GENERAL ACCESS TO TELECOMMUNICATIONS SERVICE, TELECOMMUNICATIONS EQUIPMENT AND CUSTOMER PREMISES EQUIPMENT BY PERSONS WITH DISABILITIES...

  4. New insights into consumer-oriented food products design

    NARCIS (Netherlands)

    Almeida Costa, A.I.

    2003-01-01

      To test the implementation of the most promising methods and tools associated with the early phases of a consumer-oriented approach to food product design;To improve the tested methods, or develop new ones, whenever necessary;To use the results obtained to propose research guidelines leading to

    • 3D scan to product design : Methods, techniques, and cases

      NARCIS (Netherlands)

      Lee, W.; Lee, B.; Kim, S.; Jung, H.; Jeon, E.; Choi, T.; You, H.

      2015-01-01

      3D scanning technology has derived great opportunities for ergonomic product designs. This paper is aimed to introduce various research cases and methods based on 3D scanning have conducted by an ergonomics laboratory in South Korea. Sizing systems and representative 3D models developed on

    • How Axiomatic Design can promote creativity in the design of new products

      Directory of Open Access Journals (Sweden)

      Gabriel-Santos António

      2017-01-01

      Full Text Available In product development, creativity is the driving force for doing something that leads to innovation. A typical ideation background has three subsystems: inspiration, dematerialization and recombination. The most basic concepts of Axiomatic Design, i.e. domains, hierarchies and zigzagging, as well as the two design axioms, provide a powerful framework to implement ideation, as to make creativity easier when developing a new product. The result of dematerialization is in the functional domain, which is the place where the customer needs are presented by functional requirements and purged of any kind of physical bias. The recombination creates the design parameters, at the physical domain, which are the set of elements of the design object that have been chosen to satisfy the functional requirements, into a new materialization profile of a new product. In this paper, a concrete case illustrating the above-mentioned concepts is presented.

    • Recirculation: A New Concept to Drive Innovation in Sustainable Product Design for Bio-Based Products.

      Science.gov (United States)

      Sherwood, James; Clark, James H; Farmer, Thomas J; Herrero-Davila, Lorenzo; Moity, Laurianne

      2016-12-29

      Bio-based products are made from renewable materials, offering a promising basis for the production of sustainable chemicals, materials, and more complex articles. However, biomass is not a limitless resource or one without environmental and social impacts. Therefore, while it is important to use biomass and grow a bio-based economy, displacing the unsustainable petroleum basis of energy and chemical production, any resource must be used effectively to reduce waste. Standards have been developed to support the bio-based product market in order to achieve this aim. However, the design of bio-based products has not received the same level of attention. Reported here are the first steps towards the development of a framework of understanding which connects product design to resource efficiency. Research and development scientists and engineers are encouraged to think beyond simple functionality and associate value to the potential of materials in their primary use and beyond.

    • Energy efficient design of cognitive small cells

      NARCIS (Netherlands)

      Wildemeersch, Matthias; Wildemeersch, Matthias; Quek, Tony Q.S.; Rabbachin, Alberto; Slump, Cornelis H.; Huang, Aiping; Kim, Dong-In; Mueller, P.

      2013-01-01

      Heterogeneous networks consisting of a macrocell tier and a small cell tier are considered an attractive solution to cope with the fierce increase of mobile traffic demand. Nevertheless, a massive deployment of small cell access points (SAPs) leads also to a considerable increase in energy

    • Concepts in production ecology for analysis and design of animal and plant-animal production systems

      NARCIS (Netherlands)

      Ven, van de G.W.J.; Ridder, de N.; Keulen, van H.; Ittersum, van M.K.

      2003-01-01

      The use of a hierarchy in growth factors (defining, limiting and reducing growth factors), as developed for plant production has shown its usefulness in the analysis and design of plant production systems. This hierarchy presents a theoretical framework for the analysis of biophysical conditions in

    • Knowledge productivity : designing and testing a method to diagnose knowledge productivity and plan for enhancement

      NARCIS (Netherlands)

      Stam, C.D.

      2007-01-01

      Our economy has changed from an industrial into a knowledge economy in which knowledge productivity has become the main challenge. The lack of appropriate techniques to reveal knowledge productivity hinders organizations to design effective policies aiming at improving knowledge-based performance.

    • Supplier integration for new product developments: antecedents for supplier integration in modular product designs

      NARCIS (Netherlands)

      Eggers, Justus Erich

      2016-01-01

      Original equipment manufacturers (OEMs) increasingly integrate supplier resources in new product developments (NPD) to overcome bottlenecks of resource and to create competitive advantages. At the same time, OEMs react to the new market challenges by implementing modular product designs. Despite the

    • Effect of microwell chip structure on cell microsphere production of various animal cells.

      Science.gov (United States)

      Sakai, Yusuke; Yoshida, Shirou; Yoshiura, Yukiko; Mori, Rhuhei; Tamura, Tomoko; Yahiro, Kanji; Mori, Hideki; Kanemura, Yonehiro; Yamasaki, Mami; Nakazawa, Kohji

      2010-08-01

      The formation of three-dimensional cell microspheres such as spheroids, embryoid bodies, and neurospheres has attracted attention as a useful culture technique. In this study, we investigated a technique for effective cell microsphere production by using specially prepared microchip. The basic chip design was a multimicrowell structure in triangular arrangement within a 100-mm(2) region in the center of a polymethylmethacrylate (PMMA) plate (24x24 mm(2)), the surface of which was modified with polyethylene glycol (PEG) to render it nonadhesive to cells. We also designed six similar chips with microwell diameters of 200, 300, 400, 600, 800, and 1000 microm to investigate the effect of the microwell diameter on the cell microsphere diameter. Rat hepatocytes, HepG2 cells, mouse embryonic stem (ES) cells, and mouse neural progenitor/stem (NPS) cells formed hepatocyte spheroids, HepG2 spheroids, embryoid bodies, and neurospheres, respectively, in the microwells within 5 days of culture. For all the cells, a single microsphere was formed in each microwell under all the chip conditions, and such microsphere configurations remained throughout the culture period. Furthermore, the microsphere diameters of each type of cell were strongly positively correlated with the microwell diameters of the chips, suggesting that microsphere diameter can be factitiously controlled by using different chip conditions. Thus, this chip technique is a promising cellular platform for tissue engineering or regenerative medicine research, pharmacological and toxicological studies, and fundamental studies in cell biology. Copyright 2010 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

    • Product architecture development enabling integrated re-design of mechanical products

      NARCIS (Netherlands)

      Begelinger, R.E.; Post, E.; van Houten, F.J.A.M.; Kals, H.J.J.

      1999-01-01

      Global competition forces companies to increase their competitive advantage. The design process represents an interesting area to improve the overall business performance. There are two topics involved in improving the design process. The first one is the integration of constraints from the product

    • Research of CITP-II tritium production irradiation device design

      International Nuclear Information System (INIS)

      Zhang Zhihua; Deng Yongjun; Mi Xiangmiao; Li Rundong; Liu Zhiyong

      2012-01-01

      As the core component of CITP-II, the online tritium production irradiation device is the pivotal equipment in the research on tritium production and release of tritium breeders. The design of CITP-II online tritium production irradiation device creatively makes replacing the breeders online come true; as tritium production capacity, the self-shielding factor of device, and neutron flux were studied. The influence of different load models and load thicknesses of breeders to tritium production capacity was calculated. The hydrodynamics parameters of device in solid-gas phase were computed. Thermal parameters, such as the heat power of breeders, hotspot, temperature grads distributions, utmost temperature, uneven factors, were analyzed. Creatively designed nonlinear electric heater equalized breeders' even heat power. The influence laws of the components, pressure of gap gas and carrier gas to the balance temperature were got. And the key thermal parameters were ascertained. The key thermal parameters and the changing laws were got and provide the basis for structural optimization and safety analysis. They can also be referenced for the study of breeders' tritium production and release. (authors)

    • Design for Sustainability: Current Trends in Sustainable Product Design and Development

      Directory of Open Access Journals (Sweden)

      Marcel Crul

      2009-08-01

      Full Text Available The Design for Sustainability (D4S concept outlines methodologies for making sustainable improvements (social, economic and environmental to products by applying elements of life cycle thinking. D4S builds on the work of ecodesign to include economic and social concerns, and its methodology includes both incremental and radical innovation. The United Nations Environment Programme and the Delft University of Technology, the Netherlands, in concert with key partners, work to support, illustrate, and diffuse targeted D4S demonstration efforts, including the European Commission-funded Cleaner Production for Better Products project in Vietnam, that are needed to change unsustainable consumption and production patterns.

    • Systematic Computer-Aided Framework for Sustainable Chemical Product Design

      DEFF Research Database (Denmark)

      Cignitti, Stefano; Zhang, Lei; Kalakul, Sawitree

      for CAPD, including the accuracy of the property estimation, molecular structure generation, inclusion of sustainability, process and application targets and needs in the problem formulation. CAPD has been widely utilized for the synthesis of several types of products, such as solvents, polymers, fuels...... and environmental impact. In step (3), the CAPD formulation is converted into a mixed-integer nonlinear program (MINLP) by set-up of constraints, objective and boundaries defined in step (2). In step (4), the MINLP is solved through a decomposed approach [3]. The decomposed approach breaks down the MINLP problem...... and formulated products [2]. However, for product design problems where the process needs and sustainability possess demanding constraints, the complexity of the problem is increased as the relation between product property, sustainability and process criteria is difficult to mathematically define. In this work...

    • Chemical Product Design: A new challenge of applied thermodynamics

      DEFF Research Database (Denmark)

      Abildskov, Jens; Kontogeorgis, Georgios

      2004-01-01

      Chemical products involving specialty chemicals and microstructured materials are often multicomponent systems. A number of five to 20 molecules is not unusual, comprising a range of different chemical compounds e.g. polymers, surfactants, solid particles and water. Milk is an example......, and then to outline some specific examples from our research activities in the area of thermodynamics for chemical products. The examples cover rather diverse areas such as interrelation between thermodynamic and engineering properties in detergents (surfactants), paint thermodynamics and the development of models...... try to describe and understand chemical products with traditional thermodynamic models, typically applicable to problems of petrochemical industries. The purpose of this article is two-fold: first to present some current and future challenges in thermodynamic modelling towards chemical product design...

    • Test Results From The Idaho National Laboratory Of The NASA Bi-Supported Cell Design

      Energy Technology Data Exchange (ETDEWEB)

      C Stoots; J O' Brien; T Cable

      2009-11-01

      The Idaho National Laboratory has been researching the application of solid-oxide fuel cell technology for large-scale hydrogen production. As a result, the Idaho National Laboratory has been testing various cell designs to characterize electrolytic performance. NASA, in conjunction with the University of Toledo, has developed a new cell concept with the goals of reduced weight and high power density. This paper presents results of the INL's testing of this new solid oxide cell design as an electrolyzer. Gas composition, operating voltage, and other parameters were varied during testing. Results to date show the NASA cell to be a promising design for both high power-to-weight fuel cell and electrolyzer applications.

    • Tissue-specific designs of stem cell hierarchies

      NARCIS (Netherlands)

      Visvader, Jane E.; Clevers, Hans

      2016-01-01

      Recent work in the field of stem cell biology suggests that there is no single design for an adult tissue stem cell hierarchy, and that different tissues employ distinct strategies to meet their self-renewal and repair requirements. Stem cells may be multipotent or unipotent, and can exist in

    • Tissue-specific designs of stem cell hierarchies

      NARCIS (Netherlands)

      Visvader, Jane E; Clevers, Hans

      Recent work in the field of stem cell biology suggests that there is no single design for an adult tissue stem cell hierarchy, and that different tissues employ distinct strategies to meet their self-renewal and repair requirements. Stem cells may be multipotent or unipotent, and can exist in

  1. Molecular design of materials for cell separation.

    Science.gov (United States)

    Kataoka, K

    1988-12-01

    There has been a strong demand in biomedical sciences to isolate viable cell populations with high yield and purity. An important facet of this work was to develop new polymeric adsorbent for the separation of lymphocyte subpopulations. Based on our strategy of separating cells through their differential ionic affinity toward multiphase-structured adsorbent with ionically derivatized microdomains, a series of poly(2-hydroxyethyl methacrylate)/polyamine graft copolymers (HA copolymers) was prepared. HA copolymer columns were found to show specific adsorption affinity toward B lymphocytes, and allows for separation of B and T lymphocytes in high yield and purity with a short operating time. Separation mechanism involved in the resolution of B and T lymphocytes by HA copolymer column is discussed in this paper. Further, photo-induced desorption of cells from the adsorbent derivatized with photo-responsive functional group (azobenzene group) was demonstrated to emphasize the feasibility of photo-regulated chromatography as a novel tool in cell separation technology.

  2. Switchable photosystem-II designer algae for photobiological hydrogen production

    Science.gov (United States)

    Lee, James Weifu

    2010-01-05

    A switchable photosystem-II designer algae for photobiological hydrogen production. The designer transgenic algae includes at least two transgenes for enhanced photobiological H.sub.2 production wherein a first transgene serves as a genetic switch that can controls photosystem II (PSII) oxygen evolution and a second transgene encodes for creation of free proton channels in the algal photosynthetic membrane. In one embodiment, the algae includes a DNA construct having polymerase chain reaction forward primer (302), a inducible promoter (304), a PSII-iRNA sequence (306), a terminator (308), and a PCR reverse primer (310). In other embodiments, the PSII-iRNA sequence (306) is replaced with a CF.sub.1-iRNA sequence (312), a streptomycin-production gene (314), a targeting sequence (316) followed by a proton-channel producing gene (318), or a PSII-producing gene (320). In one embodiment, a photo-bioreactor and gas-product separation and utilization system produce photobiological H.sub.2 from the switchable PSII designer alga.

  3. USE OF LEAN PRODUCTION INSTRUMENTS IN DESIGNING THE EDUCATIONAL PROCESS

    Directory of Open Access Journals (Sweden)

    Elietta P. Burnasheva

    2016-03-01

    Full Text Available Introduction: the concept of lean production seeks not a reduction of costs but complete elimination of losses that do not bring added value to the product or service. In any system, in all processes – from production and assembly, to hospitality, education, health, transport and social services – there are hidden losses. Teaching itself is a kind of production process in which a certain “product” (student acquires the added value (knowledge and skills, that is why it has become topical in educational institution to establish the working group on introduction of lean production into the learning process. The article presents the factors that are to be taken into account while designing the educational process based on the lean production principles. Materials and Methods: methods of analysis of existing system of vocational training in higher school, monitoring of the results of educational practice, modeling and experimental work in the process of analytical work were used. Results: important direction for eliminating losses in the educational process is the development of the interlinked curricula, allowing to avoid repeated study of a number of didactic units in the organization of continuous training in the system “Vocational education – Higher education”. In order to eliminate the possibility of incompetent graduate one should focus on the organisation of objective final control. Losses in education are caused by to the mismatch between labour market demand and the spectrum of areas of training in educational institutions. Discussion and Conclusions: the lean production possibilities are defined as instrumental in ensuring the organisation of “the process of lean learning”: by applying some lean production instruments such as the designing of the educational process, preventing of “faulty work” while training students, the attuning of the training system to employers’ requests, the visualisation of the education

  4. The innovation effect of user design: Exploring consumers' innovation perceptions of firms selling products designed by users

    NARCIS (Netherlands)

    M. Schreier (Martin); C. Fuchs (Christoph); D.W. Dahl (Darren)

    2012-01-01

    textabstractThe authors study consumer perceptions of firms that sell products designed by users. In contrast with the traditional design mode, in which professional designers employed by firms handle the design task, common design by users involves the firm's user community in creating new product

  5. Analysis and Design of Fuel Cell Systems for Aviation

    Directory of Open Access Journals (Sweden)

    Thomas Kadyk

    2018-02-01

    Full Text Available In this paper, the design of fuel cells for the main energy supply of passenger transportation aircraft is discussed. Using a physical model of a fuel cell, general design considerations are derived. Considering different possible design objectives, the trade-off between power density and efficiency is discussed. A universal cost–benefit curve is derived to aid the design process. A weight factor w P is introduced, which allows incorporating technical (e.g., system mass and efficiency as well as non-technical design objectives (e.g., operating cost, emission goals, social acceptance or technology affinity, political factors. The optimal fuel cell design is not determined by the characteristics of the fuel cell alone, but also by the characteristics of the other system components. The fuel cell needs to be designed in the context of the whole energy system. This is demonstrated by combining the fuel cell model with simple and detailed design models of a liquid hydrogen tank. The presented methodology and models allows assessing the potential of fuel cell systems for mass reduction of future passenger aircraft.

  6. Production of organic acids in an immobilized cell reactor using ...

    African Journals Online (AJOL)

    STORAGESEVER

    2008-09-17

    , whole cell, ... activities of free enzyme and cell may gradually decline in a bioreactor (Gui et al., 2007; ... Product. Examples. Enzymes. -Amylases, cellulase and other cellulolytic enzymes, chitinolytic enzymes, cyclodextrin,.

  7. Exploring Advertising and Product Design Sexual Implications: A Sociocultural Approach

    Directory of Open Access Journals (Sweden)

    Johannis Tsoumas

    2016-07-01

    Full Text Available This paper explores design thought and application on various fields of its domain, under the prism of the human sexuality. However the two main areas of our concern are the fields of advertising and product design which, according to the author, represent the most significant parts on which the concept of human sexuality is thoroughly applied and still remains effective. Designing under this instinctive human value, one can easily trace the importance of concepts such as charm and pleasure, which can overlap and possibly ‘beat to death’ the concepts of usability and quality of use, in many ways. The starting point of this allegory in handling and promoting new products, most of which are not directly connected with the sense of sexuality, are the advertisements. On the other hand, one will be able to find out the myth of gender and its contradictions in a certain group of products which have direct sexuality references and most of them are designed for a limited, however faithful and increasingly concerned consumer group. Thirdly, the author aims to focus on the ‘discovery’ of socially marginalized consumer groups, such as the homosexual community, by some inventive entrepreneurs and designers, for the formation of a ‘new market’ which seems to thrive in terms of profit. As this, meteoric rise, market is directly connected with analogous social changes globally, one will be able to understand its development, but also the exploitation of homosexual behaviour, via the ideological points of both sides: designers and consumers.

  8. Design, production and materials of PV powered consumer products - the case of mass production (cd-rom)

    NARCIS (Netherlands)

    Reinders, Angelina H.M.E.; Akkerman, Remko; Palz, W.; Ossenbrink, H.; Helm, P.

    2005-01-01

    Though many options exist, the application of integrated PV systems in mass produced consumer products is still unusual and rare [1]. Therefore, to date, design and manufacturing aspects of product-integrated PV systems have been explored only to a very limited extent. The requirements for the

  9. Simple electrolytic cell for production of elemental fluorine

    International Nuclear Information System (INIS)

    Dides F, M.; Padilla S, U.

    1990-01-01

    It was constructed and tested a simple electrolytic cell for the production of elemental fluorine. The fluorine production is essential in the obtainment of uranium hexafluoride, a compound for the nuclear fuel cycle. (A.C.A.S.)

  10. Recombinant Protein Production and Insect Cell Culture and Process

    Science.gov (United States)

    Spaulding, Glenn F. (Inventor); Goodwin, Thomas J. (Inventor); OConnor, Kim C. (Inventor); Francis, Karen M. (Inventor); Andrews, Angela D. (Inventor); Prewett, Tracey L. (Inventor)

    1997-01-01

    A process has been developed for recombinant production of selected polypeptides using transformed insect cells cultured in a horizontally rotating culture vessel modulated to create low shear conditions. A metabolically transformed insect cell line is produced using the culture procedure regardless of genetic transformation. The recombinant polypeptide can be produced by an alternative process using virtually infected or stably transformed insect cells containing a gene encoding the described polypeptide. The insect cells can also be a host for viral production.

  11. Microbial electrolysis cells as innovative technology for hydrogen production

    International Nuclear Information System (INIS)

    Chorbadzhiyska, Elitsa; Hristov, Georgi; Mitov, Mario; Hubenova, Yolina

    2011-01-01

    Hydrogen production is becoming increasingly important in view of using hydrogen in fuel cells. However, most of the production of hydrogen so far comes from the combustion of fossil fuels and water electrolysis. Microbial Electrolysis Cell (MEC), also known as Bioelectrochemically Assisted Microbial Reactor, is an ecologically clean, renewable and innovative technology for hydrogen production. Microbial electrolysis cells produce hydrogen mainly from waste biomass assisted by various bacteria strains. The principle of MECs and their constructional elements are reviewed and discussed. Keywords: microbial Electrolysis Cells, hydrogen production, waste biomass purification

  12. Systems metabolic engineering design: fatty acid production as an emerging case study.

    Science.gov (United States)

    Tee, Ting Wei; Chowdhury, Anupam; Maranas, Costas D; Shanks, Jacqueline V

    2014-05-01

    Increasing demand for petroleum has stimulated industry to develop sustainable production of chemicals and biofuels using microbial cell factories. Fatty acids of chain lengths from C6 to C16 are propitious intermediates for the catalytic synthesis of industrial chemicals and diesel-like biofuels. The abundance of genetic information available for Escherichia coli and specifically, fatty acid metabolism in E. coli, supports this bacterium as a promising host for engineering a biocatalyst for the microbial production of fatty acids. Recent successes rooted in different features of systems metabolic engineering in the strain design of high-yielding medium chain fatty acid producing E. coli strains provide an emerging case study of design methods for effective strain design. Classical metabolic engineering and synthetic biology approaches enabled different and distinct design paths towards a high-yielding strain. Here we highlight a rational strain design process in systems biology, an integrated computational and experimental approach for carboxylic acid production, as an alternative method. Additional challenges inherent in achieving an optimal strain for commercialization of medium chain-length fatty acids will likely require a collection of strategies from systems metabolic engineering. Not only will the continued advancement in systems metabolic engineering result in these highly productive strains more quickly, this knowledge will extend more rapidly the carboxylic acid platform to the microbial production of carboxylic acids with alternate chain-lengths and functionalities. © 2014 Wiley Periodicals, Inc.

  13. A Research Review on the Key Technologies of Intelligent Design for Customized Products

    Directory of Open Access Journals (Sweden)

    Shuyou Zhang

    2017-10-01

    Full Text Available The development of technologies such as big data and cyber-physical systems (CPSs has increased the demand for product design. Product digital design involves completing the product design process using advanced digital technologies such as geometry modeling, kinematic and dynamic simulation, multi-disciplinary coupling, virtual assembly, virtual reality (VR, multi-objective optimization (MOO, and human-computer interaction. The key technologies of intelligent design for customized products include: a description and analysis of customer requirements (CRs, product family design (PFD for the customer base, configuration and modular design for customized products, variant design for customized products, and a knowledge push for product intelligent design. The development trends in intelligent design for customized products include big-data-driven intelligent design technology for customized products and customized design tools and applications. The proposed method is verified by the design of precision computer numerical control (CNC machine tools.

  14. An Empirical Study on Design Partner Selection in Green Product Collaboration Design

    Directory of Open Access Journals (Sweden)

    Aijun Liu

    2018-01-01

    Full Text Available Green production has become an important issue in PCD (Product Collaboration Design for almost every enterprise, and will determine the sustainability of enterprises in the long term. The choice of design partner is a necessary condition in order to achieve green production. For the uncertain, fuzzy, and dynamic information such as unknown indices and weights, fuzzy semantics, and dynamic time factors in GPCD (Green Product Collaboration Design, a two-stage dynamic hybrid MADM (Multi-Attribute Decision Making approach based on fuzzy DEMATEL (Decision-Making and Trial Evaluation Laboratory, fuzzy KMA (Karnik–Mendel Algorithm, and fuzzy VIKOR (VlseKriterjumska Optimizacija I Kompromisno Resenje was proposed. In the first stage, fuzzy DEMATEL was used to determine the evaluation indices. Then, in the second stage, to accurately depict the dynamic information generated by the different phases of a product design, the dynamic evaluation method based on fuzzy theories was employed, and the weights of the indices were calculated by fuzzy KMA, then sorted by fuzzy VIKOR. Finally, a case study and a comparative analysis wre provided to illustrate the effectiveness of the proposed approach.

  15. Property Modelling and Databases in Product-Process Design

    DEFF Research Database (Denmark)

    Gani, Rafiqul; Sansonetti, Sascha

    development, however, it is necessary to have a large database of measured property data that has been checked for consistency and accuracy. The presentation will first introduce a database, in terms of its knowledge representation structure, the type and range of properties and chemical systems covered......, and their internal consistency-accuracy checks. The database includes properties of organic chemicals, polymers and ionic liquids. There are also chemical class specific database sections, such as for solvents, aroma-chemicals, surfactants and emulsifiers. The use of this property database for model development...... of the PC-SAFT is used. The developed database and property prediction models have been combined into a properties-software that allows different product-process design related applications. The presentation will also briefly highlight applications of the software for virtual product-process design...

  16. Designing Mechatronic Products - Achieving Integration by Means of Modelling Dependencies

    DEFF Research Database (Denmark)

    Torry-Smith, Jonas

    disciplines have to be combined and function in an integrated fashion. The holistic view of the product concept is sometimes missing in projects and engineering disciplines are reluctant to interact. Investigations of literature, cases from industry and practical experience are used for dissecting...... the challenges related to the integration phenomenon and evaluating how well the challenges are addressed by using the solutions in a design setting. The study shows that the available solutions only partly cover the stated challenges and that a large part of the identified solutions appear to support analysis......-related dependency’ is defined to describe relations in the product concept between functions, properties and means (solutions) appearing as a consequence of the design process. Based on three cases from industry a classification of dependencies is established, highlighting thirteen types of generic dependencies...

  17. An Integrated Methodology for Emulsified Formulated Product Design

    DEFF Research Database (Denmark)

    Mattei, Michele

    consists of three stages: the problem definition stage, the model - based design stage, and the experiment - based verification stage. In the probl em definition stage, the consumer needs are trans- lated into a set of target thermo - physical properties and into a list of categories of ingre- dients...... significantly reduce both time and cost connected to product development by doing only the necessary experi- ments , and ensuring chances for innovation . The main contribution of this project i s the development of an integrated methodology for the design of emulsified formulated products. The methodology...... formulation are measured by means of tailor - made exp eriments. The formulation is then validated or, if necessary, re- fined thanks to a systematic list of action. The problem definition stage relies on a robust knowledge base, which needs to system- atically generate quantitative, useful input information...

  18. Sustainable Product Design, Engineering and Management Education for Industrial Design Engineering

    OpenAIRE

    Boks, C.; Diehl, J.C.; Wever, R.

    2006-01-01

    Developments in the field of sustainable product design are manifold, which means that education in this field is rapidly evolving as well. In this paper, the continuously evolving portfolio of courses offered at Delft University of Technology’s Industrial Design Engineering faculty is systematically discussed, with a focus on content, course formats, assignments and lessons learned from course evaluations in recent years. It is concluded that in particular integration in existing contexts (a...

  19. Methods for Stem Cell Production and Therapy

    Science.gov (United States)

    Claudio, Pier Paolo (Inventor); Valluri, Jagan V. (Inventor)

    2015-01-01

    The present invention relates to methods for rapidly expanding a stem cell population with or without culture supplements in simulated microgravity conditions. The present invention relates to methods for rapidly increasing the life span of stem cell populations without culture supplements in simulated microgravity conditions. The present invention also relates to methods for increasing the sensitivity of cancer stem cells to chemotherapeutic agents by culturing the cancer stem cells under microgravity conditions and in the presence of omega-3 fatty acids. The methods of the present invention can also be used to proliferate cancer cells by culturing them in the presence of omega-3 fatty acids. The present invention also relates to methods for testing the sensitivity of cancer cells and cancer stem cells to chemotherapeutic agents by culturing the cancer cells and cancer stem cells under microgravity conditions. The methods of the present invention can also be used to produce tissue for use in transplantation by culturing stem cells or cancer stem cells under microgravity conditions. The methods of the present invention can also be used to produce cellular factors and growth factors by culturing stem cells or cancer stem cells under microgravity conditions. The methods of the present invention can also be used to produce cellular factors and growth factors to promote differentiation of cancer stem cells under microgravity conditions.

  20. Fuel cell design using a new heuristic method

    International Nuclear Information System (INIS)

    Perusquia, R.; Montes T, J. L.; Ortiz S, J. J.; Castillo M, A.

    2014-10-01

    In this paper a new method for the pre-design of a typical fuel cell with a structural array of 10 x 10 fuel elements for a BWR is presented. The method is based on principles of maximum dispersion and minimum peaks of local power within the array of fuel elements. The pre-design of the fuel cells is made by simulation in two dimensions (2-D) through the cells physics code CASMO-4. For this purpose of pre-design the search process is guided by an objective function which is a combination of the main neutronic parameters of the fuel cell. The results show that the method is a promising tool that could be used for the design of fuel cells for use in a nuclear plant BWR. (Author)

  1. Topography of ganglion cell production in the cat's retina

    International Nuclear Information System (INIS)

    Walsh, C.; Polley, E.H.

    1985-01-01

    The ganglion cells of the cat's retina form several classes distinguishable in terms of soma size, axon diameter, dendritic morphology, physiological properties, and central connections. Labeling with [ 3 H]thymidine shows that the ganglion cells which survive in the adult are produced as several temporally shifted, overlapping waves: medium-sized cells are produced before large cells, whereas the smallest ganglion cells are produced throughout the period of ganglion cell generation. Large cells and medium-sized cells show the same distinctive pattern of production, forming rough spirals around the area centralis. The oldest cells tend to lie superior and nasal to the area centralis, whereas cells in the inferior nasal retina and inferior temporal retina are, in general, progressively younger. Within each retinal quadrant, cells nearer the area centralis tend to be older than cells in the periphery, but there is substantial overlap. The retinal raphe divides the superior temporal quadrant into two zones with different patterns of cell addition. Superior temporal retina near the vertical meridian adds cells only slightly later than superior nasal retina, whereas superior temporal retina near the horizontal meridian adds cells very late, contemporaneously with inferior temporal retina. The broader wave of production of smaller ganglion cells seems to follow this same spiral pattern at its beginning and end. The presence of the area centralis as a nodal point about which ganglion cell production in the retinal quadrants pivots suggests that the area centralis is already an important retinal landmark even at the earliest stages of retinal development

  2. Food product design: emerging evidence for food policy.

    Science.gov (United States)

    Al-Hamdani, Mohammed; Smith, Steven

    2017-03-01

    The research on the impact of specific brand elements such as food descriptors and package colors is underexplored. We tested whether a "light" color and a "low-calorie" descriptor on food packages gain favorable consumer perception ratings as compared with regular packages. Our online experiment recruited 406 adults in a 3 (product type: Chips versus Juice versus Yoghurt) × 2 (descriptor type: regular versus low-calorie) × 2 (color type: regular versus light) mixed design. Dependent variables were sensory (evaluations of the product's nutritional value and quality), product-based (evaluations of the product's physical appeal), and consumer-based (evaluations of the potential consumers of the product) scales. "Low-calorie" descriptors were found to increase sensory ratings as compared with regular descriptors and light-colored packages received higher product-based ratings as compared with their regular-colored counterparts. Food package color and descriptors present a promising venue for understanding preventative measures against obesity.[Formula: see text].

  3. Design and Testing of Subsystems for Mo-99 Production

    Energy Technology Data Exchange (ETDEWEB)

    Gromov, R. [Argonne National Lab. (ANL), Argonne, IL (United States); Bailey, J. [Argonne National Lab. (ANL), Argonne, IL (United States); Virgo, M. [Argonne National Lab. (ANL), Argonne, IL (United States); Chemerisov, S. [Argonne National Lab. (ANL), Argonne, IL (United States); Vandegrift, G. F. [Argonne National Lab. (ANL), Argonne, IL (United States)

    2017-01-01

    Argonne National Laboratory, in cooperation with Los Alamos National Laboratory, is developing technology with NorthStar Medical Technologies to produce 99Mo from the γ,n reaction on a 100Mo target in an electron accelerator. During production runs and thermal testing of the helium-cooled target, it became obvious that a production-scale beam-line configuration would need a collimator to protect the target from accidental beam misplacement or a beam-profile change. A prototype high-power collimator and beam stop were designed and fabricated. Testing indicated that they will be able to operate at full power in the production-scale accelerator.

  4. Sustainable and Intensified Design of a Biodiesel Production Process

    DEFF Research Database (Denmark)

    Mansouri, Seyed Soheil; Ismail, Muhammad I.; Babi, Deenesh Kavi

    impact and maximum profitability is needed. In this work a computer-aided framework for process synthesis and process intensification is applied for sustainable production of biodiesel from pure/waste palm oil as the feedstock. This approach examines several biodiesel processing routes that were...... collected through available data and current technologies reported in the literature. Using this information, a generic superstructure of processing routes was created that described a network of configurations representing multiple designs for the production of biodiesel. Therefore, based on the currently...... of economic and environmental sustainability was identified. For the case of biodiesel production, the intensified process alternative turned out to be the most economical and more sustainable than other alternatives. The computer-aided methods and tools used in this work are: SustainPro (method and tool...

  5. Implementation of quality by design toward processing of food products.

    Science.gov (United States)

    Rathore, Anurag S; Kapoor, Gautam

    2017-05-28

    Quality by design (QbD) is a systematic approach that begins with predefined objectives and emphasizes product and process understanding and process control. It is an approach based on principles of sound science and quality risk management. As the food processing industry continues to embrace the idea of in-line, online, and/or at-line sensors and real-time characterization for process monitoring and control, the existing gaps with regard to our ability to monitor multiple parameters/variables associated with the manufacturing process will be alleviated over time. Investments made for development of tools and approaches that facilitate high-throughput analytical and process development, process analytical technology, design of experiments, risk analysis, knowledge management, and enhancement of process/product understanding would pave way for operational and economic benefits later in the commercialization process and across other product pipelines. This article aims to achieve two major objectives. First, to review the progress that has been made in the recent years on the topic of QbD implementation in processing of food products and second, present a case study that illustrates benefits of such QbD implementation.

  6. Integrated design strategy for product life-cycle management

    Science.gov (United States)

    Johnson, G. Patrick

    2001-02-01

    Two major trends suggest new considerations for environmentally conscious manufacturing (ECM) -- the continuation of dematerialization and the growing trend toward goods becoming services. A diversity of existing research could be integrated around those trends in ways that can enhance ECM. Major research-based achievements in information, computation, and communications systems, sophisticated and inexpensive sensing capabilities, highly automated and precise manufacturing technologies, and new materials continue to drive the phenomenon of dematerialization - the reduction of the material and energy content of per capita GDP. Knowledge is also growing about the sociology, economics, mathematics, management and organization of complex socio-economic systems. And that has driven a trend towards goods evolving into services. But even with these significant trends, the value of material, energy, information and human resources incorporated into the manufacture, use and disposal of modern products and services often far exceeds the benefits realized. Multi-disciplinary research integrating these drivers with advances in ECM concepts could be the basis for a new strategy of production. It is argued that a strategy of integrating information resources with physical and human resources over product life cycles, together with considering products as streams of service over time, could lead to significant economic payoff. That strategy leads to an overall design concept to minimize costs of all resources over the product life cycle to more fully capture benefits of all resources incorporated into modern products. It is possible by including life cycle monitoring, periodic component replacement, re-manufacture, salvage and human factor skill enhancement into initial design.

  7. Design and development of A 20 Ah Li-ion prismatic cell

    Science.gov (United States)

    Gitzendanner, R. L.; Russell, P. G.; Marsh, C.; Marsh, R. A.

    Yardney Technical Products has undertaken a program to develop large scale, 20 Ah size, lithium-ion cells for military and aerospace applications. A space efficient and adaptable parallel-plate prismatic design has been chosen for this program. Using advanced, energy dense materials, 20 Ah cells with a specific energy of 123 Wh/kg and energy density of 326 Wh/1 have been produced. Many of the design parameters for these cells were optimized using statistical design experiments with smaller test cells. These design experiments allowed the identification and optimization of important parameters in far less time than would have been required by standard, iterative, experimentation. Optimization of rate capability and extended life cycling were the primary objectives for these experiments. High discharge rates up to 2 C and extended, 100% DOD, life cycling to greater than 2000 cycles have been achieved.

  8. Arecoline decreases interleukin-6 production and induces apoptosis and cell cycle arrest in human basal cell carcinoma cells

    Energy Technology Data Exchange (ETDEWEB)

    Huang, Li-Wen [Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan (China); Hsieh, Bau-Shan; Cheng, Hsiao-Ling [Department of Biochemistry, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan (China); Hu, Yu-Chen [Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan (China); Chang, Wen-Tsan [Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan (China); Division of Hepatobiliarypancreatic Surgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung 80708, Taiwan (China); Chang, Kee-Lung, E-mail: Chang.KeeLung@msa.hinet.net [Department of Biochemistry, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan (China); Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan (China)

    2012-01-15

    Arecoline, the most abundant areca alkaloid, has been reported to decrease interleukin-6 (IL-6) levels in epithelial cancer cells. Since IL-6 overexpression contributes to the tumorigenic potency of basal cell carcinoma (BCC), this study was designed to investigate whether arecoline altered IL-6 expression and its downstream regulation of apoptosis and the cell cycle in cultured BCC-1/KMC cells. BCC-1/KMC cells and a human keratinocyte cell line, HaCaT, were treated with arecoline at concentrations ranging from 10 to 100 μg/ml, then IL-6 production and expression of apoptosis- and cell cycle progress-related factors were examined. After 24 h exposure, arecoline inhibited BCC-1/KMC cell growth and decreased IL-6 production in terms of mRNA expression and protein secretion, but had no effect on HaCaT cells. Analysis of DNA fragmentation and chromatin condensation showed that arecoline induced apoptosis of BCC-1/KMC cells in a dose-dependent manner, activated caspase-3, and decreased expression of the anti-apoptotic protein Bcl-2. In addition, arecoline induced progressive and sustained accumulation of BCC-1/KMC cells in G2/M phase as a result of reducing checkpoint Cdc2 activity by decreasing Cdc25C phosphatase levels and increasing p53 levels. Furthermore, subcutaneous injection of arecoline led to decreased BCC-1/KMC tumor growth in BALB/c mice by inducing apoptosis. This study demonstrates that arecoline has potential for preventing BCC tumorigenesis by reducing levels of the tumor cell survival factor IL-6, increasing levels of the tumor suppressor factor p53, and eliciting cell cycle arrest, followed by apoptosis. Highlights: ► Arecoline has potential to prevent against basal cell carcinoma tumorigenesis. ► It has more effectiveness on BCC as compared with a human keratinocyte cell line. ► Mechanisms involved including reducing tumor cells’ survival factor IL-6, ► Decreasing Cdc25C phosphatase, enhancing tumor suppressor factor p53, ► Eliciting G2/M

  9. NASA Alternative Orion Small Cell Battery Design Support

    Science.gov (United States)

    Haynes, Chuck

    2016-01-01

    The NASA Orion Crew Module Reference Design was produced to address large scale thermal runaway (TR) hazard with specific safety controls for the Orion Spacecraft. The design presented provides the description of a full scale battery design reference for implementation as a drop in replacement to meet all spacecraft energy requirements with compatible 120 Vdc electrical and mechanical interface using small cell technology (18650) packaging. The 32V SuperBrick incorporates unique support features and an electrical bus bar arrangement that allows cells negative can insertion into heat sink that is compressively coupled to the battery enclosure to promote good thermal management. The housing design also provides an internal flame suppression "filter tray" and positive venting path internal to the enclosure to allow hot effluent ejecta to escape in the event of single cell TR. Virtual cells (14P Banks) that are supported to provide cell spacing with interstitial materials to prevent side can failures that can produce cell to cell TR propagation. These features were successfully test in four separate TR run with the full scale DTA1 test article in February 2016. Successfully Completed Test Objectives - Four separate TR test runs with Full-Scale DTA1 housing with Two SuperBricks, Two SuperBrick Emulators All Tests resulted in "clean" gas with less than 6 C rise at Battery vent All Tests resulted in less than 2 C temperature rise on cold-plate outlet All Tests resulted in less than 6 psi pressure rise in the battery housing Test Run 1 -One neighbor cell TR, highest remaining neighbor 139 C. Ejecta shorted to bus caused prolonged additional heating, One shorted cell did experience TR after 12 minutes, remaining cells had adequate thermal margin Test Run 2 - No cell to cell propagation, highest neighbor cell 112 C; Test Run 3 - No cell to cell propagation, highest neighbor cell 96 C; Test Run 4 - No cell to cell propagation, highest neighbor cell 101 C; Primary TR testing

  10. Sustainable Chemical Processes and Products. New Design Methodology and Design Tools

    NARCIS (Netherlands)

    Korevaar, G.

    2004-01-01

    The current chemical industry is not sustainable, which leads to the fact that innovation of chemical processes and products is too often hazardous for society in general and the environment in particular. It really is a challenge to implement sustainability considerations in the design activities

  11. Health motivation and product design determine consumers' visual attention to nutrition information on food products

    OpenAIRE

    Visschers, Vivianne HM; Hess, Rebecca; Siegrist, Michael

    2017-01-01

    Abstract Objective In the present study we investigated consumers' visual attention to nutrition information on food products using an indirect instrument, an eye tracker. In addition, we looked at whether people with a health motivation focus on nutrition information on food products more than people with a taste motivation. Design Respondents were instructed to choose one of five cereals for either the kindergarten (health motivation) or the student cafeteria (taste motivation). The eye tra...

  12. Design, Modeling, and Development of Microbial Cell Factories

    KAUST Repository

    Kodzius, Rimantas

    2014-03-26

    Using Metagenomic analysis, computational modeling, single cell and genome editing technologies, we will express desired microbial genes and their networks in suitable hosts for mass production of energy, food, and fine chemicals.

  13. Alternative designs for petroleum product storage tanks for groundwater protection.

    Science.gov (United States)

    Oke Adeleke, Samson

    In developing countries, there are numerous occurrences of petroleum product spillage in groundwater. The current practice of burying storage tanks beneath the surface without adequate safety devices facilitates this phenomenon. Underground tanks rust and leak, and spilled petroleum products migrate downward. The movement of the oil in the soil depends on its viscosity and quantity, the permeability of the soil/rock, and the presence of fractures within the rock. The oil spreads laterally in the form of a thin pancake due to its lower specific gravity, and soluble components dissolve in water. The pollution plume of petroleum products and dissolved phases moves in the direction of groundwater flow in the aquifer within the pores of soil and sediments or along fractures in basement complex areas. Most communities reply heavily on groundwater for potable and industrial supplies. However, the sustainability of this resource is under threat in areas where there are filling stations as a result of significant groundwater contamination from petroleum product spillage. Drinking water becomes unpalatable when it contains petroleum products in low concentrations, and small quantities may contaminate large volumes of water. Considering the losses incurred from spillage, the cost of cleaning the aquifer, and the fact that total cleansing and attenuation is impossible, the need to prevent spillage and if it happens to prevent it from getting into the groundwater system is of paramount importance. This paper proposes alternative design procedures with a view to achieving these objectives.

  14. A Change in Envirocare's Disposal Cell Design

    International Nuclear Information System (INIS)

    Rogers, T.

    2002-01-01

    Envirocare of Utah, Inc. operates a Low Level Radioactive Waste (LLRW) and 11e. disposal facility in the Utah west dessert. Envirocare disposes of LLRW in above ground cells. A seven-foot excavation lined with two feet of clay comprises the cell floor. Approximately 22 feet of waste is then placed in the cell in one-foot thick compacted lifts. The cover system consists of a nine-foot clay radon barrier and three-foot rock erosion barrier. This is required to prevent radon emissions at the surface of the radon barrier from exceeding 20 pCi/m2s, the radon release standard in Criterion 6 of 10 CFR 40. The required thickness of the current clay radon barrier cover was based on the original radon flux model used to evaluate the safety of Envirocare's proposed LLRW and 11e.(2) license operations. Because of the lack of actual measurements, universally conservative values were used for the long-term moisture content and the radon diffusion coefficients of the waste and radon barrier material. Since receiving its license, Envirocare has collected a number of samples from the radon barrier and waste material to determine their actual radon attenuation characteristics, including the long-term moisture content and the associated radon diffusion coefficient. In addition, radon flux measurements have been performed to compare the model calculations with the calculated results. The results from these analyses indicate that the initial modeling input parameters, specifically the long-term moisture content and the radon diffusion coefficient, are more conservative than that needed to ensure compliance with the applicable regulatory requirements

  15. Broadband back grating design for thin film solar cells

    KAUST Repository

    Janjua, Bilal

    2013-01-01

    In this paper, design based on tapered circular grating structure was studied, to provide broadband enhancement in thin film amorphous silicon solar cells. In comparison to planar structure an absorption enhancement of ~ 7% was realized.

  16. Design & Fabrication of a High-Voltage Photovoltaic Cell

    Energy Technology Data Exchange (ETDEWEB)

    Felder, Jennifer; /North Carolina State U. /SLAC

    2012-09-05

    Silicon photovoltaic (PV) cells are alternative energy sources that are important in sustainable power generation. Currently, applications of PV cells are limited by the low output voltage and somewhat low efficiency of such devices. In light of this fact, this project investigates the possibility of fabricating high-voltage PV cells on float-zone silicon wafers having output voltages ranging from 50 V to 2000 V. Three designs with different geometries of diffusion layers were simulated and compared in terms of metal coverage, recombination, built-in potential, and conduction current density. One design was then chosen and optimized to be implemented in the final device design. The results of the simulation serve as a feasibility test for the design concept and provide supportive evidence of the effectiveness of silicon PV cells as high-voltage power supplies.

  17. A Comprehensive Framework for Surfactant Selection and Design for Emulsion Based Chemical Product Design

    DEFF Research Database (Denmark)

    Mattei, Michele; Kontogeorgis, Georgios; Gani, Rafiqul

    2014-01-01

    The manufacture of emulsified products is of increasing interest in the consumer oriented chemical indus-try. Several cosmetic, house-hold and pharmaceutical products are in the emulsified form when soldand/or they are expected to form an emulsion when used. Therefore, there is a need for the dev......The manufacture of emulsified products is of increasing interest in the consumer oriented chemical indus-try. Several cosmetic, house-hold and pharmaceutical products are in the emulsified form when soldand/or they are expected to form an emulsion when used. Therefore, there is a need...... ingredientsis consequently necessary to tackle this problem with computer-aided methods and tools. A compre-hensive framework for the selection and design of surfactants, the main responsible for the formationand the stability of emulsions, is presented here together with the modeling of the cloud point, a key......-property of nonionic surfactants, with a group-contribution model. The mathematical formulation of astandard product design problem is presented, together with the list of both the pure component prop-erties (related to nonionic surfactants) and the mixture properties (relevant to the overall products asan emulsion...

  18. Systematic Methodology for Design of Tailor-Made Blended Products: Fuels and Other Blended Products

    DEFF Research Database (Denmark)

    Yunus, Nor Alafiza Binti

    A tailor-made blended liquid product is defined as a formulation of various chemicals in the liquid state to obtain a liquid mixture with a specific set of desired characteristics and qualities. Examples of blended liquid products are synthetic fuels and lubricants. This type of products is very ...... methodology and tools was tested through two case studies. In the first case study, two different gasoline blend problems have been solved. In the second case study, four different lubricant design problems have been solved....

  19. Food product design. An integrated approach (2nd edition completely revised)

    NARCIS (Netherlands)

    Linnemann, A.R.; Schroën, C.G.P.H.; Boekel, van M.A.J.S.

    2011-01-01

    'Food product design ­ An integrated approach' deals with food product design from a technological perspective. It presents creative techniques for the innovation process and structured methodologies to translate consumer wishes into product properties based on Quality Function Deployment.

  20. Biodiesel production from vegetable oil: Process design, evaluation and optimization

    Directory of Open Access Journals (Sweden)

    Kianimanesh Hamid Reza

    2017-09-01

    Full Text Available To investigate the effect of reactor performance/configuration of biodiesel production on process parameters (mass & energy consumption, required facilities etc., two diverse production processes (from vegetable oil were implemented/designed using Aspen HYSYS V7.2. Two series reactors were taken into account where overall conversion was set to be 97.7% and 70% in first and second processes respectively. Comparative analysis showed that an increase in conversion yield caused to consumption reduction of oil, methanol, cold energy and hot energy up to 9.1%, 22%, 67.16% and 60.28% respectively; further, a number of facilities (e.g. boiler, heat exchanger, distillation tower were reduced. To reduce mass & energy consumption, mass/heat integration method was employed. Applying integration method showed that in the first design, methanol, cold and hot energy were decreased by 49.81%, 17.46% and 36.17% respectively; while in the second design, oil, methanol, cold and hot energy were decreased by 9%, 60.57% 19.62% and 36.58% respectively.

  1. Engineered mammalian cells for production of recombinant proteins

    DEFF Research Database (Denmark)

    2017-01-01

    The present invention relates to mammalian cells modified to provide for improved expression of a recombinant protein of interest. In particular, the invention relates to CHO cells and other host cells in which the expression of one or more endogenous secreted proteins has been disrupted, as well...... as to the preparation, identification and use of such cells in the production of recombinant proteins.......The present invention relates to mammalian cells modified to provide for improved expression of a recombinant protein of interest. In particular, the invention relates to CHO cells and other host cells in which the expression of one or more endogenous secreted proteins has been disrupted, as well...

  2. In vitro production of azadirachtin from cell suspension cultures of ...

    Indian Academy of Sciences (India)

    PRAKASH KUMAR G

    to affect the growth and metabolism of cultured cells, and have been studied extensively in different species in .... Specific growth rate of neem cell suspensions in altered nitrate: ammonium ratio. MS, Murashige and Skoog medium; MS medium with .... Stimulated caffeine production has been reported in Coffea arabica cell ...

  3. Method for reducing ammonium and lactate production in cho cells

    DEFF Research Database (Denmark)

    2018-01-01

    The present invention relates to modified producer cells for improved production of therapeutic proteins. Specifically, the inventors have found that removing genes involved in amino acid catabolism in Chinese Hamster Ovary (CHO) cells improves the cell growth and viability and likely also...

  4. Harnessing cell-material interaction to control cell fate: design ...

    Indian Academy of Sciences (India)

    Physical (topography, stiffness) and chemical instructions encoded in the extracellular environment govern cell behaviour. Understanding these interactions as they operate in native extracellular matrix and capturing the complexity of the spatiotemporal presentation of multiple such signals in synthetic scaffolds is a key to ...

  5. Compact hydrogen production systems for solid polymer fuel cells

    Science.gov (United States)

    Ledjeff-Hey, K.; Formanski, V.; Kalk, Th.; Roes, J.

    Generally there are several ways to produce hydrogen gas from carbonaceous fuels like natural gas, oil or alcohols. Most of these processes are designed for large-scale industrial production and are not suitable for a compact hydrogen production system (CHYPS) in the power range of 1 kW. In order to supply solid polymer fuel cells (SPFC) with hydrogen, a compact fuel processor is required for mobile applications. The produced hydrogen-rich gas has to have a low level of harmful impurities; in particular the carbon monoxide content has to be lower than 20 ppmv. Integrating the reaction step, the gas purification and the heat supply leads to small-scale hydrogen production systems. The steam reforming of methanol is feasible at copper catalysts in a low temperature range of 200-350°C. The combination of a small-scale methanol reformer and a metal membrane as purification step forms a compact system producing high-purity hydrogen. The generation of a SPFC hydrogen fuel gas can also be performed by thermal or catalytic cracking of liquid hydrocarbons such as propane. At a temperature of 900°C the decomposition of propane into carbon and hydrogen takes place. A fuel processor based on this simple concept produces a gas stream with a hydrogen content of more than 90 vol.% and without CO and CO2.

  6. Health motivation and product design determine consumers' visual attention to nutrition information on food products.

    Science.gov (United States)

    Visschers, Vivianne H M; Hess, Rebecca; Siegrist, Michael

    2010-07-01

    In the present study we investigated consumers' visual attention to nutrition information on food products using an indirect instrument, an eye tracker. In addition, we looked at whether people with a health motivation focus on nutrition information on food products more than people with a taste motivation. Respondents were instructed to choose one of five cereals for either the kindergarten (health motivation) or the student cafeteria (taste motivation). The eye tracker measured their visual attention during this task. Then respondents completed a short questionnaire. Laboratory of the ETH Zurich, Switzerland. Videos and questionnaires from thirty-two students (seventeen males; mean age 24.91 years) were analysed. Respondents with a health motivation viewed the nutrition information on the food products for longer and more often than respondents with a taste motivation. Health motivation also seemed to stimulate deeper processing of the nutrition information. The student cafeteria group focused primarily on the other information and did this for longer and more often than the health motivation group. Additionally, the package design affected participants' nutrition information search. Two factors appear to influence whether people pay attention to nutrition information on food products: their motivation and the product's design. If the package design does not sufficiently facilitate the localization of nutrition information, health motivation can stimulate consumers to look for nutrition information so that they may make a more deliberate food choice.

  7. Design of microdevices for long-term live cell imaging

    International Nuclear Information System (INIS)

    Chen, Huaying; Nordon, Robert E; Rosengarten, Gary; Li, Musen

    2012-01-01

    Advances in fluorescent live cell imaging provide high-content information that relates a cell's life events to its ancestors. An important requirement to track clonal growth and development is the retention of motile cells derived from an ancestor within the same microscopic field of view for days to weeks, while recording fluorescence images and controlling the mechanical and biochemical microenvironments that regulate cell growth and differentiation. The aim of this study was to design a microwell device for long-term, time-lapse imaging of motile cells with the specific requirements of (a) inoculating devices with an average of one cell per well and (b) retaining progeny of cells within a single microscopic field of view for extended growth periods. A two-layer PDMS microwell culture device consisting of a parallel-plate flow cell bonded on top of a microwell array was developed for cell capture and clonal culture. Cell deposition statistics were related to microwell geometry (plate separation and well depth) and the Reynolds number. Computational fluid dynamics was used to simulate flow in the microdevices as well as cell–fluid interactions. Analysis of the forces acting upon a cell was used to predict cell docking zones, which were confirmed by experimental observations. Cell–fluid dynamic interactions are important considerations for design of microdevices for long-term, live cell imaging. The analysis of force and torque balance provides a reasonable approximation for cell displacement forces. It is computationally less intensive compared to simulation of cell trajectories, and can be applied to a wide range of microdevice geometries to predict the cell docking behavior. (paper)

  8. the production of mouse embryonic stem cells

    Indian Academy of Sciences (India)

    MADU

    Much research and lively debate focuses on the potential value of embryonic stem (ES) cells for regenerative medicine, the ethical issues raised by the use of human embryos to generate them, and the efforts presently made to circumvent this problem. The preparation of human ES cells in 1998 (Thomson et al 1998) ...

  9. Design of nanocatalysts for green hydrogen production from bioethanol.

    Science.gov (United States)

    Bion, Nicolas; Duprez, Daniel; Epron, Florence

    2012-01-09

    Bioethanol is an interesting feedstock that may be used for hydrogen production by steam or autothermal reforming. However, the impurities (heavy alcohols, esters, acids, N compounds) contained in the raw feedstock require a costly purification, as they have a dramatic impact on catalyst activity and stability. Thus, a method that can utilize the raw feedstock without severe degradation of the catalyst would be desirable. In this Minireview, the composition of bioethanol from first and second generation biomass, the reactions involved in the catalytic ethanol steam reforming process and the design of catalysts adapted for hydrogen production from a real bioethanol feed are surveyed. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  10. Challenges in Capitalizing Knowledge in Innovative Product Design Process

    Directory of Open Access Journals (Sweden)

    Ines Saad

    2010-08-01

    Full Text Available Capitalizing on company's knowledge is increasingly being recognized in a private organizations environment since managing knowledge productivity is considered a source of competitive advantage. In this paper we present a generalization of GAMETH framework, that play an important role in identifying crucial knowledge used and created in innovative product design process. Thus, we have developed a method based on three phases. In the first phase, we have used GAMETH to identify the set of "reference knowledge". During the second phase, decision rules are inferred, through rough sets theory, from decision assignments provided by the decision maker(s. In the third phase, a multicriteria classification of "potential crucial knowledge" is performed on the basis of the decision rules that have been collectively identified by the decision maker(s.

  11. Fabrication of Complex Optical Components From Mold Design to Product

    CERN Document Server

    Riemer, Oltmann; Gläbe, Ralf

    2013-01-01

    High quality optical components for consumer products made of glass and plastic are mostly fabricated by replication. This highly developed production technology requires several consecutive, well-matched processing steps called a "process chain" covering all steps from mold design, advanced machining and coating of molds, up to the actual replication and final precision measurement of the quality of the optical components. Current market demands for leading edge optical applications require high precision and cost effective parts in large volumes. For meeting these demands it is necessary to develop high quality process chains and moreover, to crosslink all demands and interdependencies within these process chains. The Transregional Collaborative Research Center "Process chains for the replication of complex optical elements" at Bremen, Aachen and Stillwater worked extensively and thoroughly in this field from 2001 to 2012. This volume will present the latest scientific results for the complete process chain...

  12. A product-service system approach to telehealth application design.

    Science.gov (United States)

    Flores-Vaquero, Paul; Tiwari, Ashutosh; Alcock, Jeffrey; Hutabarat, Windo; Turner, Chris

    2016-06-01

    A considerable proportion of current point-of-care devices do not offer a wide enough set of capabilities if they are to function in any telehealth system. There is a need for intermediate devices that lie between healthcare devices and service networks. The development of an application is suggested that allows for a smartphone to take the role of an intermediate device. This research seeks to identify the telehealth service requirements for long-term condition management using a product-service system approach. The use of product-service system has proven to be a suitable methodology for the design and development of telehealth smartphone applications. © The Author(s) 2014.

  13. Additive manufacturing of metals the technology, materials, design and production

    CERN Document Server

    Yang, Li; Baughman, Brian; Godfrey, Donald; Medina, Francisco; Menon, Mamballykalathil; Wiener, Soeren

    2017-01-01

    This book offers a unique guide to the three-dimensional (3D) printing of metals. It covers various aspects of additive, subtractive, and joining processes used to form three-dimensional parts with applications ranging from prototyping to production. Examining a variety of manufacturing technologies and their ability to produce both prototypes and functional production-quality parts, the individual chapters address metal components and discuss some of the important research challenges associated with the use of these technologies. As well as exploring the latest technologies currently under development, the book features unique sections on electron beam melting technology, material lifting, and the importance this science has in the engineering context. Presenting unique real-life case studies from industry, this book is also the first to offer the perspective of engineers who work in the field of aerospace and transportation systems, and who design components and manufacturing networks. Written by the leadin...

  14. Conceiving product ideas in an initial and uncertain design situation

    DEFF Research Database (Denmark)

    Hansen, Claus Thorp; Andreasen, Mogens Myrup

    2006-01-01

    As a consequence of the global market’s demand for innovation industrial companies need employees with well-articulated innovation competences. Conceptualisation may be seen as the core activity of innovation: the concept is the new idea, the new initiative, the new organising, or the new approach......, which carry innovation. Traditional approaches to conceptualisation are no longer sufficient. Too many engineering designers have practiced or cultivated a one-sided product quality focus and too many believe that creativity can compensate for a lack of knowledge and insight into the users’ perception......, and explorative view of conceptualisation. We propose an ontology for ideation in an initial and uncertain design situation, and we point out a number of approaches to conceptualisation to break with the traditional way of working. In its present form the paper has to be seen as a speculation. The authors believe...

  15. The Integration Method of Ceramic Arts in the Product Design

    Science.gov (United States)

    Shuxin, Wang

    2018-03-01

    As one of the four ancient civilization countries, the firing technology of ceramic invented by China has made a great contribution to the progress and development of human society. In modern life, even the development of technology still needs the ceramics, there are large number of artists who take the ceramics as carrier active in the field of contemporary art. The ceramics can be seen everywhere in our daily life, this paper mainly discusses the integration means of ceramic art in the product design.

  16. DESIGN OF A PRODUCTION PLANT CARMINE AND ANNATO

    OpenAIRE

    Erazo E., Raymundo; Universidad Nacional Mayor de San Marcos, Facultad de Química e Ingeniería Química Departamento de Análisis y Diseño de Procesos Av. Venezuela s/n - Lima Perú; Caso H., M. J.; Universidad Nacional Mayor de San Marcos, Facultad de Química e Ingeniería Química Departamento de Análisis y Diseño de Procesos Av. Venezuela s/n - Lima Perú

    2014-01-01

    In this work we present the design of a plant to produce Carmine and Annatto from Cochineal and annatto tree, respectively. The piant will be installed in Lima and all the equípments of the process will be build from stainless steel 316 with sanitary finishing. We estimate an inversion of US$ 351 650 to produce 12,7 Kg of Carmine and 15,5 Kg of Annatto per day. The quality of the products are among the required standards by the international market. The rentability of the process is 20,45% fo...

  17. Advanced Turbine Systems (ATS) program conceptual design and product development

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1996-08-31

    Achieving the Advanced Turbine Systems (ATS) goals of 60% efficiency, single-digit NO{sub x}, and 10% electric power cost reduction imposes competing characteristics on the gas turbine system. Two basic technical issues arise from this. The turbine inlet temperature of the gas turbine must increase to achieve both efficiency and cost goals. However, higher temperatures move in the direction of increased NO{sub x} emission. Improved coatings and materials technologies along with creative combustor design can result in solutions to achieve the ultimate goal. GE`s view of the market, in conjunction with the industrial and utility objectives, requires the development of Advanced Gas Turbine Systems which encompass two potential products: a new aeroderivative combined-cycle system for the industrial market, and a combined-cycle system for the utility sector that is based on an advanced frame machine. The GE Advanced Gas Turbine Development program is focused on two specific products: (1) a 70 MW class industrial gas turbine based on the GE90 core technology utilizing an innovative air cooling methodology; (2) a 200 MW class utility gas turbine based on an advanced Ge heavy-duty machine utilizing advanced cooling and enhancement in component efficiency. Both of these activities required the identification and resolution of technical issues critical to achieving ATS goals. The emphasis for the industrial ATS was placed upon innovative cycle design and low emission combustion. The emphasis for the utility ATS was placed on developing a technology base for advanced turbine cooling, while utilizing demonstrated and planned improvements in low emission combustion. Significant overlap in the development programs will allow common technologies to be applied to both products. GE Power Systems is solely responsible for offering GE products for the industrial and utility markets.

  18. Designer exosomes produced by implanted cells intracerebrally deliver therapeutic cargo for Parkinson's disease treatment.

    Science.gov (United States)

    Kojima, Ryosuke; Bojar, Daniel; Rizzi, Giorgio; Hamri, Ghislaine Charpin-El; El-Baba, Marie Daoud; Saxena, Pratik; Ausländer, Simon; Tan, Kelly R; Fussenegger, Martin

    2018-04-03

    Exosomes are cell-derived nanovesicles (50-150 nm), which mediate intercellular communication, and are candidate therapeutic agents. However, inefficiency of exosomal message transfer, such as mRNA, and lack of methods to create designer exosomes have hampered their development into therapeutic interventions. Here, we report a set of EXOsomal transfer into cells (EXOtic) devices that enable efficient, customizable production of designer exosomes in engineered mammalian cells. These genetically encoded devices in exosome producer cells enhance exosome production, specific mRNA packaging, and delivery of the mRNA into the cytosol of target cells, enabling efficient cell-to-cell communication without the need to concentrate exosomes. Further, engineered producer cells implanted in living mice could consistently deliver cargo mRNA to the brain. Therapeutic catalase mRNA delivery by designer exosomes attenuated neurotoxicity and neuroinflammation in in vitro and in vivo models of Parkinson's disease, indicating the potential usefulness of the EXOtic devices for RNA delivery-based therapeutic applications.

  19. FFTF in-containment cell liner design and installation experience

    International Nuclear Information System (INIS)

    Umek, A.M.; Swenson, L.D.

    1980-01-01

    Design features and liner construction techniques are discussed. Cell leak-rate tests and the methods used to locate and repair leaks are described. A brief analysis of the overall experience at FFTF is provided, with recommendations for future plant designs

  20. Scan cell design for enhanced delay fault testability

    NARCIS (Netherlands)

    van Brakel, Gerrit; van Brakel, G.; Xing, Yizi; Xing, Y.; Kerkhoff, Hans G.

    1992-01-01

    Problems in testing scannable sequential circuits for delay faults are addressed. Modifications to improve circuit controllability and observability for the testing of delay faults are implemented efficiently in a scan cell design. A layout on a gate array is designed and evaluated for this scan

  1. 8. Industrial Design: The Roles and Factors of Aesthetics, Modeling, Styling, Product Brand and Branding in Design/Design Education

    Directory of Open Access Journals (Sweden)

    Efer Obasuyi Osa-Francis

    2017-03-01

    Full Text Available This paper examined the roles and factors of Aesthetics viz-a-viz those of modeling, styling and the product Brand (Branding as viewed against their fundamental relevance as core ingredients for the actualization of any Design initiative and creative endeavour. These roles, influences and place of Aesthetics (and its allied components, makes up the very soul, content and essence of what the product is all about. Its Form, Shape, Morphological / physical attributes and characteristics. The paper established that the aesthetic components of beauty, colour, shape, (form fonts, (lettering or typography and all other general Aesthetic Ambience gives the given product the desired quality/beauty that it exudes to help determine its marketability / profitability as a Brand. The paper also reviewed the aspects /implications of these roles and influences on Design Education alongside highlighting the potentials of the Design Educator - learner experience, while exploiting the Teachers ability to effectively harmonise the various approaches and practices of a functional Design-led Educational programme viz-a-viz piloting such talents/skills achieved to practicable and useful ends as professionals in Design and Technology. The paper also seeks to establish that the roles and factors of modeling styling and Branding of the product which is best exemplified through the adaptation of Design inputs and features such as ornamentation, edge delineation, texture movement/flow of linear form, symmetry or Assymetry of shapes, colour, granularity (of matter/particles large or small or coarseness, play of light (sunlight and shade, transcendence (state of being or existence of a product beyond the limits of material experience and that of total harmony. The paper recommends that for any product Brand to succeed it must incorporate all of the above components together with the inclusion of the other qualities that makes up the intangible content of the Brand such as

  2. Center for Intelligent Fuel Cell Materials Design

    Energy Technology Data Exchange (ETDEWEB)

    Santurri, P.R., (Chemsultants International); Hartmann-Thompson, C.; Keinath, S.E. (Michigan Molecular Inst.)

    2008-08-26

    The goal of this work was to develop a composite proton exchange membrane utilizing 1) readily available, low cost materials 2) readily modified and 3) easily processed to meet the chemical, mechanical and electrical requirements of high temperature PEM fuel cells. One of the primary goals was to produce a conducting polymer that met the criteria for strength, binding capability for additives, chemical stability, dimensional stability and good conductivity. In addition compatible, specialty nanoparticles were synthesized to provide water management and enhanced conductivity. The combination of these components in a multilayered, composite PEM has demonstrated improved conductivity at high temperatures and low humidity over commercially available polymers. The research reported in this final document has greatly increased the knowledge base related to post sulfonation of chemically and mechanically stable engineered polymers (Radel). Both electrical and strength factors for the degree of post sulfonation far exceed previous data, indicating the potential use of these materials in suitable proton exchange membrane architectures for the development of fuel cells. In addition compatible, hydrophilic, conductive nano-structures have been synthesized and incorporated into unique proton exchange membrane architectures. The use of post sulfonation for the engineered polymer and nano-particle provide cost effective techniques to produce the required components of a proton exchange membrane. The development of a multilayer proton exchange membrane as described in our work has produced a highly stable membrane at 170°C with conductivities exceeding commercially available proton exchange membranes at high temperatures and low humidity. The components and architecture of the proton exchange membrane discussed will provide low cost components for the portable market and potentially the transportation market. The development of unique components and membrane architecture

  3. Design and Simulation of a Quaternary Memory Cell based on a Physical Memristor

    DEFF Research Database (Denmark)

    Nannarelli, Alberto; Taylor, Jonathan

    2016-01-01

    Memristors were theorized more than fifty years ago, but only recently physical devices with memristor’s behavior have been fabricated and shipped. In this work, we experiment on one of these physical memristors by designing a memristorbased memory cell, implementing the cell, and testing it. Our...... experiments demonstrate that the memristor technology is not yet mature for practical applications, but, nevertheless, when production will provide reliable and dependable devices, memristorbased memory systems may replace CMOS memories with some advantages....

  4. Urokinase production by electrophoretically separated cultured human embryonic kidney cells

    Science.gov (United States)

    Kunze, M. E.; Plank, L. D.; Giranda, V.; Sedor, K.; Todd, P. W.

    1985-01-01

    Urokinase is a plasminogen activator found in urine. Relatively pure preparations have been tested in Europe, Japan and the United States for the treatment of deep vein thrombosis and other dangerous blood clots. Human embryonic kidney cell cultures have been found to produce urokinase at much higher concentrations, but less than 5% of the cells in typical cultures are producers. Since human diploid cells become senescent in culture the selection of clones derived from single cells will not provide enough material to be useful, so a bulk purification method is needed for the isolation of urokinase producing cell populations. Preparative cell electrophoresis was chosen as the method, since evidence exists that human embryonic cell cultures are richly heterogeneous with respect to electrophoretic mobility, and preliminary electrophoretic separations on the Apollo-Soyuz space flight produced cell populations that were rich in urokinase production. Similarly, erythropoietin is useful in the treatment of certain anemias and is a kidney cell duct, and electrophoretically enriched cell populations producing this product have been reported. Thus, there is a clear need for diploid human cells that produce these products, and there is evidence that such cells should be separable by free-flow cell electrophoresis.

  5. Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells.

    Science.gov (United States)

    D'Amour, Kevin A; Bang, Anne G; Eliazer, Susan; Kelly, Olivia G; Agulnick, Alan D; Smart, Nora G; Moorman, Mark A; Kroon, Evert; Carpenter, Melissa K; Baetge, Emmanuel E

    2006-11-01

    Of paramount importance for the development of cell therapies to treat diabetes is the production of sufficient numbers of pancreatic endocrine cells that function similarly to primary islets. We have developed a differentiation process that converts human embryonic stem (hES) cells to endocrine cells capable of synthesizing the pancreatic hormones insulin, glucagon, somatostatin, pancreatic polypeptide and ghrelin. This process mimics in vivo pancreatic organogenesis by directing cells through stages resembling definitive endoderm, gut-tube endoderm, pancreatic endoderm and endocrine precursor--en route to cells that express endocrine hormones. The hES cell-derived insulin-expressing cells have an insulin content approaching that of adult islets. Similar to fetal beta-cells, they release C-peptide in response to multiple secretory stimuli, but only minimally to glucose. Production of these hES cell-derived endocrine cells may represent a critical step in the development of a renewable source of cells for diabetes cell therapy.

  6. Radiation damage of gallium arsenide production cells

    Science.gov (United States)

    Mardesich, N.; Joslin, D.; Garlick, J.; Lillington, D.; Gillanders, M.; Cavicchi, B.; Scott-Monck, J.; Kachare, R.; Anspaugh, B.

    1987-01-01

    High efficiency liquid phase epitaxy (LPE) gallium arsenide cells were irradiated with 1 Mev electrons up to fluences of 1 times 10 to the 16th power cm-2. Measurements of spectral response and dark and illuminated I-V data were made at each fluence and then, using computer codes, the experimental data was fitted to gallium arsenide cell models. In this way it was possible to determine the extent of the damage, and hence damage coefficients in both the emitter and base of the cell.

  7. Lipopolysaccharide induces IFN-γ production in human NK cells

    Directory of Open Access Journals (Sweden)

    Leonid M Kanevskiy

    2013-01-01

    Full Text Available NK cells have been shown to play a regulatory role in sepsis. According to the current view, NK cells become activated via macrophages or dendritic cells primed by lipopolysaccharide (LPS. Recently TLR4 gene expression was detected in human NK cells suggesting the possibility of a direct action of LPS on NK cells. In this study, effects of LPS on NK cell cytokine production and cytotoxicity were studied using highly purified human NK cells. LPS induced IFN-γ production in the presence of IL-2 in cell populations containing >98% CD56+ cells. Surprisingly, in the same experiments LPS decreased NK cell degranulation. No significant expression of markers related to blood dendritic cells, monocytes or T or B lymphocytes in the NK cell preparations was observed; the portions of HLA-DRbright, CD14+, CD3+ and CD20+ cells amounted to less than 0.1% within the cell populations. No more than 0.2% of NK cells were shown to be slightly positive for surface TLR4 in our experimental system, although intracellular staining revealed moderate amounts of TLR4 inside the NK cell population. These cells were negative for surface CD14, the receptor participating in LPS recognition by TLR4. Incubation of NK cells with IL-2 or/and LPS did not lead to an increase in TLR4 surface expression. TLR4–CD56+ NK cells isolated by cell sorting secreted IFN-γ in response to LPS. Antibody to TLR4 did not block the LPS-induced increase in IFN-γ production. We have also shown that Re-form of LPS lacking outer core oligosaccharide and O-antigen induces less cytokine production in NK cells than full length LPS. We speculate that the polysaccharide fragments of LPS molecule may take part in LPS-induced IFN-γ production by NK cells. Collectively our data suggest the existence of a mechanism of LPS direct action on NK cells distinct from established TLR4-mediated signaling.

  8. A kinetic model for flavonoid production in tea cell culture.

    Science.gov (United States)

    Shibasaki-Kitakawa, Naomi; Iizuka, Yasuhiro; Takahashi, Atsushi; Yonemoto, Toshikuni

    2017-02-01

    As one of the strategies for efficient production of a metabolite from cell cultures, a kinetic model is very useful tool to predict productivity under various culture conditions. In this study, we propose a kinetic model for flavonoid production in tea cell culture based on the cell life cycle and expression of PAL, the gene encoding phenylalanine ammonia-lyase (PAL)-the key enzyme in flavonoid biosynthesis. The flavonoid production rate was considered to be related to the amount of active PAL. Synthesis of PAL was modelled based on a general gene expression/translation mechanism, including the transcription of DNA encoding PAL into mRNA and the translation of PAL mRNA into the PAL protein. The transcription of DNA was assumed to be promoted at high light intensity and suppressed by a feedback regulatory mechanism at high flavonoid concentrations. In the model, mRNA and PAL were considered to self-decompose and to be lost by cell rupture. The model constants were estimated by fitting the experimental results obtained from tea cell cultures under various light intensities. The model accurately described the kinetic behaviors of dry and fresh cell concentrations, glucose concentration, cell viability, PAL specific activity, and flavonoid content under a wide range of light intensities. The model simulated flavonoid productivity per medium under various culture conditions. Therefore, this model will be useful to predict optimum culture conditions for maximum flavonoid productivity in cultured tea cells.

  9. PV modules, using color solar cells, designed for building

    Energy Technology Data Exchange (ETDEWEB)

    Ishikawa, N.; Yamashita, H.; Goda, S. [Daido Hoxan Inc., Chitose, Hokkaido (Japan). Chitose Research Lab.] [and others

    1994-12-31

    The designs and structures of a photovoltaic module to be installed in a curtain wall were examined with the object of sharply reducing PV power generation costs. As for the design, solar cells of different colors were produced and an opinion survey of designers and other construction-related persons was conducted. Renderings based on color solar cells were prepared using computer graphics. In general, the output of cells decreases for colors other than shades of dark blue. However, there is a good possibility that greater importance will be put on design, including color and surface condition, than on output. In this case, the market share may expand and as a result, the cost may drop. As for the structure, various materials that can be used for a building-material-integrated-module were investigated and methods to install PV modules into building materials were examined. Moreover, experimental module samples fitted with stainless steel sheets and aluminum-sash frames were made.

  10. Towards immersive designing of production processes using virtual reality techniques

    Directory of Open Access Journals (Sweden)

    Domagoj Buzjak

    2018-03-01

    Full Text Available The article provides a novel approach to the implementation of virtual reality within planning and design of manual processes and systems. The use of hardware and software required to perform different production - especially assembly - tasks in a virtual environment, using CAD parts as interactive elements, is presented. Considering the CAD parts, the format conversion problem is comprehensively described and solved using format conversion software to overcome the present poor data connectivity between the CAD system and VR hardware and software. Two examples of work processes have been made in a virtual environment: peg-in-hole and wall socket assembly. In the latter case, the traditional planning approach of manual assembly tasks using predetermined motion time system MTM-2 has been compared with a modern approach in which the assembly task is fully performed within a virtual environment. The comparison comprises a discussion on the assembly task execution times. In addition, general and specific advantages and disadvantages that arise in the immersive designing of production processes using virtual reality are presented, as well as reflections on teamwork and collaborative man-machine work. Finally, novel technologies are proposed to overcome the main problems that occur when implementing VR, such as time-consuming scene defining or tedious CAD software data conversion.

  11. Adaptive designs based on the truncated product method

    Directory of Open Access Journals (Sweden)

    Neuhäuser Markus

    2005-09-01

    Full Text Available Abstract Background Adaptive designs are becoming increasingly important in clinical research. One approach subdivides the study into several (two or more stages and combines the p-values of the different stages using Fisher's combination test. Methods Alternatively to Fisher's test, the recently proposed truncated product method (TPM can be applied to combine the p-values. The TPM uses the product of only those p-values that do not exceed some fixed cut-off value. Here, these two competing analyses are compared. Results When an early termination due to insufficient effects is not appropriate, such as in dose-response analyses, the probability to stop the trial early with the rejection of the null hypothesis is increased when the TPM is applied. Therefore, the expected total sample size is decreased. This decrease in the sample size is not connected with a loss in power. The TPM turns out to be less advantageous, when an early termination of the study due to insufficient effects is possible. This is due to a decrease of the probability to stop the trial early. Conclusion It is recommended to apply the TPM rather than Fisher's combination test whenever an early termination due to insufficient effects is not suitable within the adaptive design.

  12. Critical role of NKT cells in posttransplant alloantibody production.

    Science.gov (United States)

    Zimmerer, J M; Swamy, P; Sanghavi, P B; Wright, C L; Abdel-Rasoul, M; Elzein, S M; Brutkiewicz, R R; Bumgardner, G L

    2014-11-01

    We previously reported that posttransplant alloantibody production in CD8-deficient hosts is IL-4+ CD4+ T cell-dependent and IgG1 isotype-dominant. The current studies investigated the hypothesis that IL-4-producing natural killer T cells (NKT cells) contribute to maximal alloantibody production. To investigate this, alloantibody levels were examined in CD8-deficient WT, CD1d KO and Jα18 KO transplant recipients. We found that the magnitude of IgG1 alloantibody production was critically dependent on the presence of type I NKT cells, which are activated by day 1 posttransplant. Unexpectedly, type I NKT cell contribution to enhanced IgG1 alloantibody levels was interferon-γ-dependent and IL-4-independent. Cognate interactions between type I NKT and B cells alone do not stimulate alloantibody production. Instead, NKT cells appear to enhance maturation of IL-4+ CD4+ T cells. To our knowledge, this is the first report to substantiate a critical role for type I NKT cells in enhancing in vivo antibody production in response to endogenous antigenic stimuli. © Copyright 2014 The American Society of Transplantation and the American Society of Transplant Surgeons.

  13. Absorbent products for urinary/faecal incontinence: a comparative evaluation of key product designs.

    Science.gov (United States)

    Fader, M; Cottenden, A; Getliffe, K; Gage, H; Clarke-O'Neill, S; Jamieson, K; Green, N; Williams, P; Brooks, R; Malone-Lee, J

    2008-07-01

    To compare the performance and cost-effectiveness of the key absorbent product designs to provide a more solid basis for guiding selection and purchase. Also to carry out the first stage in the development of a quality of life (QoL) instrument for measuring the impact of absorbent product use on users' lives. Three clinical trials focused on the three biggest market sectors. Each trial had a similar crossover design in which each participant tested all products within their group in random order. SETTING, PARTICIPANTS AND INTERVENTIONS: In Trial 1, 85 women with light urinary incontinence living in the community tested three products from each of the four design categories available (total of 12 test products): disposable inserts (pads); menstrual pads; washable pants with integral pad; and washable inserts. In Trial 2a, 85 moderate/heavily incontinent adults (urinary or urinary/faecal) living in the community (49 men and 36 women) tested three (or two) products from each of the five design categories available (total of 14 test products): disposable inserts (with mesh pants); disposable diapers (nappies); disposable pull-ups (similar to toddlers' trainer pants); disposable T-shaped diapers (nappies with waist-band); and washable diapers. All products were provided in a daytime and a (mostly more absorbent) night-time variant. In these first two trials, the test products were selected on the basis of data from pilot studies. In Trial 2b, 100 moderate/heavily incontinent adults (urinary or urinary/faecal) living in 10 nursing homes (27 men and 73 women) evaluated one product from each of the four disposable design categories from Trial 2a. Products were selected on the basis of product performance in Trial 2a and, again, day time and night-time variants were provided. The first phase of developing a QoL tool for measuring the impact of using different pad designs was carried out by interviewing participants from Trials 1 and 2a. Product performance (e.g. comfort

  14. Optimizing PHB and Protease Production by Box Behnken Design

    Directory of Open Access Journals (Sweden)

    Amro Abd al fattah Amara

    2013-04-01

    Full Text Available Mixed culture is more suitable to adapt more flexible fermentation process and produce different product simultaneously. In this study a mixed Bacillus culture was investigated for their ability to produce the bioplastic "Polyhydroxybutyrate" and both of the mesophilic and the thermophilic proteases in one flask. Box-Behnken experimental design was used. The produced amount of PHB has been increased significantly. Meanwhile there is a competition between PHB and proteases. The maximum produced amount of PHB using Box-Behnken design was 2.82 g/l/48 h with protease activity equal to 41.9 Units/ml/48 h for thermophilic proteases and 99.65 Units/ml/48 h for mesophilic proteases. Excel solver was used for extra-optimization for the optimum conditions obtained from Box-Behnken experiments and its model. The maximum PHB obtained after using Excel solver was 2.88 g/l/48 h. The maximum mesophilic and thermophilic activities obtained at the same PHB production conditions were 175.68 and 243.38 Units/ml respectively. The model accuracy as obtained from Excel solver was 118.8%, which prove the power of the experimental design in optimizing such complicated process. The strategies used in this study are recommended for the production of PHB and different proteases simultaneously using Bacillus mixed culture. ABSTRAK: Kultur campuran adalah lebih sesuai bagi proses penapaian yang fleksibel dan ia boleh menghasilkan produk yang berbeza secara serentak. Dalam kajian ini keupayaan  menghasilkan "Polyhydroxybutyrate" bioplastik serta mesofilik dan termofilik protease dalam satu flask oleh  kultur Bacillus campuran telah disiasat. Eksperimen rekabentuk Box-Behnken telah digunakan. Jumlah PHB yang dikeluarkan meningkat dengan ketara dan terdapat persaingan antara PHB dan protease. Jumlah keluaran PHB maksima menggunakan rekabentuk Box-Behnken adalah 2.82 g/l/48 jam dengan aktiviti protease sama dengan 41.9 Unit/ml/48 jam untuk protease termofilik dan 99.65 Unit

  15. Viral vaccines and their manufacturing cell substrates: New trends and designs in modern vaccinology.

    Science.gov (United States)

    Rodrigues, Ana F; Soares, Hugo R; Guerreiro, Miguel R; Alves, Paula M; Coroadinha, Ana S

    2015-09-01

    Vaccination is one of the most effective interventions in global health. The worldwide vaccination programs significantly reduced the number of deaths caused by infectious agents. A successful example was the eradication of smallpox in 1979 after two centuries of vaccination campaigns. Since the first variolation administrations until today, the knowledge on immunology has increased substantially. This knowledge combined with the introduction of cell culture and DNA recombinant technologies revolutionized vaccine design. This review will focus on vaccines against human viral pathogens, recent developments on vaccine design and cell substrates used for their manufacture. While the production of attenuated and inactivated vaccines requires the use of the respective permissible cell substrates, the production of recombinant antigens, virus-like particles, vectored vaccines and chimeric vaccines requires the use - and often the development - of specific cell lines. Indeed, the development of novel modern viral vaccine designs combined with, the stringent safety requirements for manufacture, and the better understanding on animal cell metabolism and physiology are increasing the awareness on the importance of cell line development and engineering areas. A new era of modern vaccinology is arriving, offering an extensive toolbox to materialize novel and creative ideas in vaccine design and its manufacture. Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  16. Design and operation of a continuous integrated monoclonal antibody production process.

    Science.gov (United States)

    Steinebach, Fabian; Ulmer, Nicole; Wolf, Moritz; Decker, Lara; Schneider, Veronika; Wälchli, Ruben; Karst, Daniel; Souquet, Jonathan; Morbidelli, Massimo

    2017-09-01

    The realization of an end-to-end integrated continuous lab-scale process for monoclonal antibody manufacturing is described. For this, a continuous cultivation with filter-based cell-retention, a continuous two column capture process, a virus inactivation step, a semi-continuous polishing step (twin-column MCSGP), and a batch-wise flow-through polishing step were integrated and operated together. In each unit, the implementation of internal recycle loops allows to improve the performance: (a) in the bioreactor, to simultaneously increase the cell density and volumetric productivity, (b) in the capture process, to achieve improved capacity utilization at high productivity and yield, and (c) in the MCSGP process, to overcome the purity-yield trade-off of classical batch-wise bind-elute polishing steps. Furthermore, the design principles, which allow the direct connection of these steps, some at steady state and some at cyclic steady state, as well as straight-through processing, are discussed. The setup was operated for the continuous production of a commercial monoclonal antibody, resulting in stable operation and uniform product quality over the 17 cycles of the end-to-end integration. The steady-state operation was fully characterized by analyzing at the outlet of each unit at steady state the product titer as well as the process (HCP, DNA, leached Protein A) and product (aggregates, fragments) related impurities. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1303-1313, 2017. © 2017 American Institute of Chemical Engineers.

  17. A REVIEW: A MODEL of CULTURAL ASPECTS for SUSTAINABLE PRODUCT DESIGN

    Directory of Open Access Journals (Sweden)

    Ihwan Ghazali,

    2012-04-01

    Full Text Available Product design stages are important to consider critically in production. Generally, product design that shall be created by designer, should consider what the customer wants and needs. Nowadays issues, product design does not only consider the “wants and needs” of user, but also how the design can be created by embedding sustainability aspects in the product. Culture is also one of the important aspects which need to be considered in product design as culture affects the way users respond to the product. This paper aims to develop a new model for design development, in which the aspects of culture are incorporated into sustainable product design. By reviewing the existing literature, the authors attempt to identify the gaps of the existing papers, which illustrate how culture affects sustainable product design. Recent papers have only shown that culture influences product design, but they do not explore sustainability and the culture aspects in product design. Due to these gaps, it is therefore important to create a model which will assist designers to elicit sustainable product design based on cultural aspects. In summary, designers need to reflect on the “wants and needs” of users. The framework presented in this paper can be integrated into designers’ and companies’ decision-making during product design development.

  18. Integrated Computer-aided Framework for Sustainable Chemical Product Design and Evaluation

    DEFF Research Database (Denmark)

    Kalakul, Sawitree; Cignitti, Stefano; Zhang, Lei

    2016-01-01

    This work proposes an integrated model-based framework for chemical product design and evaluation based on which the software, VPPD-Lab (The Virtual Product-Process Design Laboratory) has been developed. The framework allows the following options: (1) design a product using design templates, such...... of the templates for three case studies: (i) the design of a refrigeration cycle, (ii) a mixture/blend design problem involving lubricant design and (iii) a tailor-made product design of jet-fuels (blended chemical products).......This work proposes an integrated model-based framework for chemical product design and evaluation based on which the software, VPPD-Lab (The Virtual Product-Process Design Laboratory) has been developed. The framework allows the following options: (1) design a product using design templates......, such as, single molecule products, formulated products, blended products, emulsified products and devices; (2) analyze the product by performing virtual experiments (product property and performance calculations); (3) create and add new product property and product performance models; (4) create new...

  19. Compressed gas domestic aerosol valve design using high viscous product

    Directory of Open Access Journals (Sweden)

    A Nourian

    2016-10-01

    Full Text Available Most of the current universal consumer aerosol products using high viscous product such as cooking oil, antiperspirants, hair removal cream are primarily used LPG (Liquefied Petroleum Gas propellant which is unfriendly environmental. The advantages of the new innovative technology described in this paper are: i. No butane or other liquefied hydrocarbon gas is used as a propellant and it replaced with Compressed air, nitrogen or other safe gas propellant. ii. Customer acceptable spray quality and consistency during can lifetime iii. Conventional cans and filling technology There is only a feasible energy source which is inert gas (i.e. compressed air to replace VOCs (Volatile Organic Compounds and greenhouse gases, which must be avoided, to improve atomisation by generating gas bubbles and turbulence inside the atomiser insert and the actuator. This research concentrates on using "bubbly flow" in the valve stem, with injection of compressed gas into the passing flow, thus also generating turbulence. The new valve designed in this investigation using inert gases has advantageous over conventional valve with butane propellant using high viscous product (> 400 Cp because, when the valving arrangement is fully open, there are negligible energy losses as fluid passes through the valve from the interior of the container to the actuator insert. The use of valving arrangement thus permits all pressure drops to be controlled, resulting in improved control of atomising efficiency and flow rate, whereas in conventional valves a significant pressure drops occurs through the valve which has a complex effect on the corresponding spray.

  20. Eco-marketing and eco-design of products

    Directory of Open Access Journals (Sweden)

    Filipović Jovan

    2007-01-01

    Full Text Available Environmental marketing (Eco-marketing, as a relatively new movement, emerged at the beginning of the 20th century, is in the growing phase of its development. Eco-labeling and other ways/types of eco-marketing are attracting more attention of consumers, since they are aware of the necessity of nature conservation and environmental protection. There has been a lot of misunderstanding and miss leadings in interpretation and substantiation of ecomarketing claims in previous period of time. One of the most concrete improvements in eco-marketing was appearance and establishment of ISO 14020 guidelines. ISO 14020 guidelines are integral part of ISO 14000 series of standards, which could be implemented in eco-marketing. At the same time, development of "green" products and marketing of "green" products, are direct positive contributions to resources reduction, environmental protection and sustainable economic development. Companies designing this kind of products can expect better position on the market, improvement of competitiveness, reduction of expenses (less raw materials, less waste, less harmful effects on the environment, raising corporate social responsibility, higher export.

  1. Computer Aided Product Service Systems Design : Service CAD and Its integration with Life Cycle Simulation

    NARCIS (Netherlands)

    Komoto, H.

    2009-01-01

    Integration of product design into service design, or vice versa, is considered to bring more efficient and effective value addition. Besides EcoDesign tools and methods, a methodology to design such an integration of products and services from a systemic perspective, or product-service systems

  2. Cell fusion in tumor progression: the isolation of cell fusion products by physical methods

    Directory of Open Access Journals (Sweden)

    Vincitorio Massimo

    2011-09-01

    Full Text Available Abstract Background Cell fusion induced by polyethylene glycol (PEG is an efficient but poorly controlled procedure for obtaining somatic cell hybrids used in gene mapping, monoclonal antibody production, and tumour immunotherapy. Genetic selection techniques and fluorescent cell sorting are usually employed to isolate cell fusion products, but both procedures have several drawbacks. Results Here we describe a simple improvement in PEG-mediated cell fusion that was obtained by modifying the standard single-step procedure. We found that the use of two PEG undertreatments obtains a better yield of cell fusion products than the standard method, and most of these products are bi- or trinucleated polykaryocytes. Fusion rate was quantified using fluorescent cell staining microscopy. We used this improved cell fusion and cell isolation method to compare giant cells obtained in vitro and giant cells obtained in vivo from patients with Hodgkin's disease and erythroleukemia. Conclusions In the present study we show how to improve PEG-mediated cell fusion and that cell separation by velocity sedimentation offers a simple alternative for the efficient purification of cell fusion products and to investigate giant cell formation in tumor development.

  3. Studio in Advertising Design, Fashion Design and Illustration, Product Design, Stage Design. Volume 3: Advanced Elective Courses in Art for Grades 10, 11, or 12.

    Science.gov (United States)

    New York State Education Dept., Albany. Bureau of Secondary Curriculum Development.

    The document provides teaching guidelines and information on advance elective courses in a studio art program for grades 10, 11, and 12. The courses are presented in four sections: (1) studio in advertising design--advertising and production, lettering, illustrating, and color reproduction; (2) studio in fashion design and illustration--elements…

  4. Towards the rational design of synthetic cells with prescribed population dynamics

    Science.gov (United States)

    Dalchau, Neil; Smith, Matthew J.; Martin, Samuel; Brown, James R.; Emmott, Stephen; Phillips, Andrew

    2012-01-01

    The rational design of synthetic cell populations with prescribed behaviours is a long-standing goal of synthetic biology, with the potential to greatly accelerate the development of biotechnological applications in areas ranging from medical research to energy production. Achieving this goal requires well-characterized components, modular implementation strategies, simulation across temporal and spatial scales and automatic compilation of high-level designs to low-level genetic parts that function reliably inside cells. Many of these steps are incomplete or only partially understood, and methods for integrating them within a common design framework have yet to be developed. Here, we address these challenges by developing a prototype framework for designing synthetic cells with prescribed population dynamics. We extend the genetic engineering of cells (GEC) language, originally developed for programming intracellular dynamics, with cell population factors such as cell growth, division and dormancy, together with spatio-temporal simulation methods. As a case study, we use our framework to design synthetic cells with predator–prey interactions that, when simulated, produce complex spatio-temporal behaviours such as travelling waves and spatio-temporal chaos. An analysis of our design reveals that environmental factors such as density-dependent dormancy and reduced extracellular space destabilize the population dynamics and increase the range of genetic variants for which complex spatio-temporal behaviours are possible. Our findings highlight the importance of considering such factors during the design process. We then use our analysis of population dynamics to inform the selection of genetic parts, which could be used to obtain the desired spatio-temporal behaviours. By identifying, integrating and automating key stages of the design process, we provide a computational framework for designing synthetic systems, which could be tested in future laboratory studies

  5. Automated platform for designing multiple robot work cells

    Science.gov (United States)

    Osman, N. S.; Rahman, M. A. A.; Rahman, A. A. Abdul; Kamsani, S. H.; Bali Mohamad, B. M.; Mohamad, E.; Zaini, Z. A.; Rahman, M. F. Ab; Mohamad Hatta, M. N. H.

    2017-06-01

    Designing the multiple robot work cells is very knowledge-intensive, intricate, and time-consuming process. This paper elaborates the development process of a computer-aided design program for generating the multiple robot work cells which offer a user-friendly interface. The primary purpose of this work is to provide a fast and easy platform for less cost and human involvement with minimum trial and errors adjustments. The automated platform is constructed based on the variant-shaped configuration concept with its mathematical model. A robot work cell layout, system components, and construction procedure of the automated platform are discussed in this paper where integration of these items will be able to automatically provide the optimum robot work cell design according to the information set by the user. This system is implemented on top of CATIA V5 software and utilises its Part Design, Assembly Design, and Macro tool. The current outcomes of this work provide a basis for future investigation in developing a flexible configuration system for the multiple robot work cells.

  6. Effect of failures and repairs on multiple cell production lines

    Energy Technology Data Exchange (ETDEWEB)

    Legato, P.; Bobbio, A.; Roberti, L.

    1989-01-01

    This paper examines a production line composed of multiple stages, or cells, which are passed in sequential order to arrive to the final product. Two possible coordination disciplines are considered, namely: the classical tandem arrangement of sequential working centers with input buffer and the kanban scheme, considered the Japanese shop floor realization of the Just-In-Time (JIT) manifacturing approach. The production line is modelled and analysed by means of Stochastic Petri Nets (SPN). Finally an analysis is made of the possibility that the working cells can incur failure/repair cycles perturbing the production flow of the line and thus reduce performance indices.

  7. Nox1-dependent superoxide production controls colon adenocarcinoma cell migration.

    Science.gov (United States)

    Sadok, Amine; Bourgarel-Rey, Véronique; Gattacceca, Florence; Penel, Claude; Lehmann, Maxime; Kovacic, Hervé

    2008-01-01

    Reactive oxygen species are well-known mediators of various biological responses. Recently, new homologues of the catalytic subunit of NADPH oxidase have been discovered in non-phagocytic cells. These new homologues (Nox1-Nox5) produce low levels of superoxides compared to the phagocytic homologue Nox2/gp91phox. Using Nox1 siRNA, we show that Nox1-dependent superoxide production affects the migration of HT29-D4 colonic adenocarcinoma cells on collagen-I. Nox1 inhibition or down-regulation led to a decrease of superoxide production and alpha 2 beta 1 integrin membrane availability. An addition of arachidonic acid stimulated Nox1-dependent superoxide production and HT29-D4 cell migration. Pharmacological evidences using phospholipase A2, lipoxygenases and protein kinase C inhibitors show that upstream regulation of Nox1 relies on arachidonic acid metabolism. Inhibition of 12-lipoxygenase decreased basal and arachidonic acid induced Nox1-dependent superoxide production and cell migration. Migration and ROS production inhibited by a 12-lipoxygenase inhibitor were restored by the addition of 12(S)-HETE, a downstream product of 12-lipoxygenase. Protein kinase C delta inhibition by rottlerin (and also GO6983) prevented Nox1-dependent superoxide production and inhibited cell migration, while other protein kinase C inhibitors were ineffective. We conclude that Nox1 activation by arachidonic acid metabolism occurs through 12-lipoxygenase and protein kinase C delta, and controls cell migration by affecting integrin alpha 2 subunit turn-over.

  8. Design, Modeling, Fabrication & Characterization of Industrial Si Solar Cells

    Science.gov (United States)

    Chowdhury, Ahrar Ahmed

    Photovoltaic is a viable solution towards meeting the energy demand in an ecofriendly environment. To ensure the mass access in photovoltaic electricity, cost effective approach needs to be adapted. This thesis aims towards substrate independent fabrication process in order to achieve high efficiency cost effective industrial Silicon (Si) solar cells. Most cost-effective structures, such as, Al-BSF (Aluminum Back Surface Field), FSF (Front Surface Field) and bifacial cells are investigated in detail to exploit the efficiency potentials. First off, we introduced two-dimensional simulation model to design and modeling of most commonly used Si solar cells in today's PV arena. Best modelled results of high efficiency Al-BSF, FSF and bifacial cells are 20.50%, 22% and 21.68% respectively. Special attentions are given on the metallization design on all the structures in order to reduce the Ag cost. Furthermore, detail design and modeling were performed on FSF and bifacial cells. The FSF cells has potentials to gain 0.42%abs efficiency by combining the emitter design and front surface passivation. The prospects of bifacial cells can be revealed with the optimization of gridline widths and gridline numbers. Since, bifacial cells have metallization on both sides, a double fold cost saving is possible via innovative metallization design. Following modeling an effort is undertaken to reach the modelled result in fabrication the process. We proposed substrate independent fabrication process aiming towards establishing simultaneous processing sequences for both monofacial and bifacial cells. Subsequently, for the contact formation cost effective screen-printed technology is utilized throughout this thesis. The best Al-BSF cell attained efficiency ˜19.40%. Detail characterization was carried out to find a roadmap of achieving >20.50% efficiency Al-BSF cell. Since, n-type cell is free from Light Induced degradation (LID), recently there is a growing interest on FSF cell. Our

  9. Design of a Rotary Kiln for Production of Sarooj

    Directory of Open Access Journals (Sweden)

    A. W. Hago

    2008-12-01

    Full Text Available Sarooj is a local term used for pozzolana. It has been used extensively as a hydraulic binder in buildings, hydraulic installations, forts and castles, and in the renovation of historical monuments. It is produced by burning specific clay soil that possesses adequate quantities of silica, alumina and iron oxides. In the past, the material was produced using a large amount of energy most of which is wasted by blowing winds. The burning system was usually thermally inefficient, and the output is rarely checked for quality. Test on products produced by using the traditional method may yield poor quality Sarooj due to inadequate burning or over burning or even insufficient grinding in the end. In this research, extensive experimentation has been performed on the method of production of Sarooj to determine the factors influencing Sarooj quality. Based on these factors, a new method of production is proposed. In this method, a fully mobile kiln was designed to produce Sarooj on the site. The kiln can produce 200 kg/h of Sarooj, and can be installed within one hour of arriving on the site. It can be towed by a tractor or a normal vehicle to the desired location, connected to the natural gas supply and the electric power. The construction is light, compact, and easy to start and shut down. In this paper, the plant is described in detail, together with some preliminary results of testing done on Sarooj produced by the kiln. Full details of tests on the product of the kiln will be given in a subsequent paper.

  10. Plant cell walls throughout evolution: towards a molecular understanding of their design principles

    Energy Technology Data Exchange (ETDEWEB)

    Sarkar, Purbasha; Bosneaga, Elena; Auer, Manfred

    2009-02-16

    Throughout their life, plants typically remain in one location utilizing sunlight for the synthesis of carbohydrates, which serve as their sole source of energy as well as building blocks of a protective extracellular matrix, called the cell wall. During the course of evolution, plants have repeatedly adapted to their respective niche,which is reflected in the changes of their body plan and the specific design of cell walls. Cell walls not only changed throughout evolution but also are constantly remodelled and reconstructed during the development of an individual plant, and in response to environmental stress or pathogen attacks. Carbohydrate-rich cell walls display complex designs, which together with the presence of phenolic polymers constitutes a barrier for microbes, fungi, and animals. Throughout evolution microbes have co-evolved strategies for efficient breakdown of cell walls. Our current understanding of cell walls and their evolutionary changes are limited as our knowledge is mainly derived from biochemical and genetic studies, complemented by a few targeted yet very informative imaging studies. Comprehensive plant cell wall models will aid in the re-design of plant cell walls for the purpose of commercially viable lignocellulosic biofuel production as well as for the timber, textile, and paper industries. Such knowledge will also be of great interest in the context of agriculture and to plant biologists in general. It is expected that detailed plant cell wall models will require integrated correlative multimodal, multiscale imaging and modelling approaches, which are currently underway.

  11. 'Fair-wind gas cell' - a new concept of a buffer gas cell design

    CERN Document Server

    Varentsov, V L

    2003-01-01

    A new concept of the buffer gas cell design is presented for the first time. For fast and efficient transport of stopped ions through the gas cell and afterwards to guide them out of the cell, the use of an intensive compulsory buffer gas flow through the cell combined with an RF-funnel structure is suggested. The operation of this type of gas cell that we call the fair-wind gas cell has been investigated by means of detailed computer simulations. Results of calculations for big size gas cell at 1 bar He buffer gas pressure are presented and discussed.

  12. Optimizing eukaryotic cell hosts for protein production through systems biotechnology and genome-scale modeling.

    Science.gov (United States)

    Gutierrez, Jahir M; Lewis, Nathan E

    2015-07-01

    Eukaryotic cell lines, including Chinese hamster ovary cells, yeast, and insect cells, are invaluable hosts for the production of many recombinant proteins. With the advent of genomic resources, one can now leverage genome-scale computational modeling of cellular pathways to rationally engineer eukaryotic host cells. Genome-scale models of metabolism include all known biochemical reactions occurring in a specific cell. By describing these mathematically and using tools such as flux balance analysis, the models can simulate cell physiology and provide targets for cell engineering that could lead to enhanced cell viability, titer, and productivity. Here we review examples in which metabolic models in eukaryotic cell cultures have been used to rationally select targets for genetic modification, improve cellular metabolic capabilities, design media supplementation, and interpret high-throughput omics data. As more comprehensive models of metabolism and other cellular processes are developed for eukaryotic cell culture, these will enable further exciting developments in cell line engineering, thus accelerating recombinant protein production and biotechnology in the years to come. Copyright © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  13. Design of a novel magnetic platform for cell manipulation

    Science.gov (United States)

    Lucarini, Gioia; Iacovacci, Veronica; Gouveia, Pedro J.; Ricotti, Leonardo; Menciassi, Arianna

    2018-02-01

    Cell manipulation tasks, especially in lab-on-a-chip applications for personalized medicine, could greatly benefit from mobile untethered microdevices able to wirelessly navigate in fluidic environments by means of magnetic fields. In this paper, the design, fabrication and testing of a magnetic platform enabling the controlled locomotion and immersion of microrobots placed at the air/liquid interface is proposed and exploited for cell manipulation. The proposed microrobot consists of a polymeric magnetic thin film that acts as cell transporter and a specific coating strategy, devised to enhance a safe cancer cell adhesion to the magnetic film. Experimental results demonstrated an overall cell viability and a fine control of magnetic microrobot locomotion. The proposed technologies are promising in view of future cell manipulation tasks for personalized medicine applications.

  14. The application of product architecture in determining the concept of mini hydrogen cell for petrol powered internal combustion engine

    Science.gov (United States)

    Nidzamuddin, M. Y.; Nadzirah, T. S.; Juffrizal, K.; Zulfattah, Z. M.; Tan, C. F.; Taha, M. M.; Hidayah, I.; Hilwa, M. Z.

    2015-05-01

    Product architecture is a method to translate the physical element of the functional requirement within the product system and describe the connection between these physical elements. Physical element will be interpreted through parts, component or subassemblies. Method of product architecture is an effective way in determined the conceptual design because it is not only considered the way of the product to be designed but it also focused on how the product will be made, used and even maintaining the product. This paper presents the methodology of the design and development of mini hydrogen cell for petrol powered internal combustion engine through the product architecture method. This method is applied based on the four stages of the product concept development process which is product element, product cluster, product geometry and the morphological chart. From this method, the best option of the concept is selected.

  15. A pesquisa sobre design e cinema: o design de produção Research on design and cinema: production design

    Directory of Open Access Journals (Sweden)

    Mauro Alejandro Baptista

    2008-11-01

    Full Text Available Este artigo traça um breve panorama da pesquisa na área de design e cinema e aponta um novo caminho: a pesquisa de design de produção, termo que antigamente era chamado de direção de arte, que é o estudo da disposição de cenários, móveis e objetos e atores. Uma das hipóteses trabalhadas é que as técnicas de finalização de imagem digital favorecem a passagem da direção de arte para o conceito de design de produção. Outra hipótese explorada pelo artigo é a pesquisa histórica do termo design de produção e sua relação com seu antecessor, a direção de arte. Research on design and cinema: production design — This article gives a brief overview of research in the field of design and cinema, and points to a new path: research of production design. This term used to be known as art direction, which consists of the study of sets, props, objects and actors. One of the hypotheses propounded here is that the techniques of digital image post-production favor the shift from art direction to production design. The second hypothesis explored here is the historical research of the term production design and its relation with its predecessor — art direction.

  16. Challenges in Design of an Orientation free Micro Direct Methanol Fuel Cell (µDMFC)

    DEFF Research Database (Denmark)

    Omidvarnia, Farzaneh; Hansen, Hans Nørgaard; Hales, Jan Harry

    2014-01-01

    The need for increasing the energy density of the power sources for portable electronic applications is getting increasingly important. Hearing aid devices are among the most demanding portable products and require power generators with high energy generation capability. In the current paper...... the challenges in design and manufacturing of a micro direct methanol fuel cell (μDMFC) as the power generator in hearing aid devices is investigated. Among the different challenges in design for μDMFC, the CO2 bubble management and orientation independency of the cell are addressed by proposing a spring loaded...

  17. Enhancement of Diosgenin Production in Plantlet and Cell Cultures ...

    African Journals Online (AJOL)

    Enhancement of Diosgenin Production in Plantlet and Cell Cultures of Dioscorea zingiberensis by Palmarumycin C13 from the Endophytic fungus, Berkleasmium sp. Dzf12. Y Mou, K Zhou, D Xu, R Yu, J Li, C Yin, L Zhou ...

  18. Electrocatalysis research for fuel cells and hydrogen production

    CSIR Research Space (South Africa)

    Mathe, MK

    2012-01-01

    Full Text Available The CSIR undertakes research in the Electrocatalysis of fuel cells and for hydrogen production. The Hydrogen South Africa (HySA) strategy supports research on electrocatalysts due to their importance to the national beneficiation strategy. The work...

  19. Teaching Design in Television Production Technology: The Twelve Steps of Preproduction

    Science.gov (United States)

    Harrison, Henry L. (Hal), III; Loveland, Thomas

    2009-01-01

    Extensive planning must be used to produce television programs. Students must develop sound design practices and understand these attributes of design in their production planning. Through the design and planning processes involved in television production, students learn that design is a creative process, and that there is no perfect design, but…

  20. 77 FR 69381 - Designation of Product Categories for Federal Procurement

    Science.gov (United States)

    2012-11-19

    ... services in critical and industrial product cleaning and the other was from an individual citizen. The... procurement program: Agricultural spray adjuvants; animal cleaning products; deodorants; dethatcher products... when purchasing products and services. Other Preferred Procurement Programs. Federal procurement...

  1. Fuel cell mining vehicles: design, performance and advantages

    International Nuclear Information System (INIS)

    Betournay, M.C.; Miller, A.R.; Barnes, D.L.

    2003-01-01

    The potential for using fuel cell technology in underground mining equipment was discussed with reference to the risks associated with the operation of hydrogen vehicles, hydrogen production and hydrogen delivery systems. This paper presented some of the initiatives for mine locomotives and fuel cell stacks for underground environments. In particular, it presents the test results of the first applied industrial fuel cell vehicle in the world, a mining and tunneling locomotive. This study was part of an international initiative managed by the Fuel Cell Propulsion Institute which consists of several mining companies, mining equipment manufacturers, and fuel cell technology developers. Some of the obvious benefits of fuel cells for underground mining operations include no exhaust gases, lower electrical costs, significantly reduced maintenance, and lower ventilation costs. Another advantage is that the technology can be readily automated and computer-based for tele-remote operations. This study also quantified the cost and operational benefits associated with fuel cell vehicles compared to diesel vehicles. It is expected that higher vehicle productivity could render fuel cell underground vehicles cost-competitive. 6 refs., 1 tab

  2. Large-scale production of diesel-like biofuels - process design as an inherent part of microorganism development.

    Science.gov (United States)

    Cuellar, Maria C; Heijnen, Joseph J; van der Wielen, Luuk A M

    2013-06-01

    Industrial biotechnology is playing an important role in the transition to a bio-based economy. Currently, however, industrial implementation is still modest, despite the advances made in microorganism development. Given that the fuels and commodity chemicals sectors are characterized by tight economic margins, we propose to address overall process design and efficiency at the start of bioprocess development. While current microorganism development is targeted at product formation and product yield, addressing process design at the start of bioprocess development means that microorganism selection can also be extended to other critical targets for process technology and process scale implementation, such as enhancing cell separation or increasing cell robustness at operating conditions that favor the overall process. In this paper we follow this approach for the microbial production of diesel-like biofuels. We review current microbial routes with both oleaginous and engineered microorganisms. For the routes leading to extracellular production, we identify the process conditions for large scale operation. The process conditions identified are finally translated to microorganism development targets. We show that microorganism development should be directed at anaerobic production, increasing robustness at extreme process conditions and tailoring cell surface properties. All the same time, novel process configurations integrating fermentation and product recovery, cell reuse and low-cost technologies for product separation are mandatory. This review provides a state-of-the-art summary of the latest challenges in large-scale production of diesel-like biofuels. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  3. Green perspective in food industry production line design: A review

    Science.gov (United States)

    Xian, C. Y.; Sin, T. C.; Liyana, M. R. N.; Awang, A.; Fathullah, M.

    2017-09-01

    The design of green manufacturing process in food industries is currently a hot research topic in the multidisciplinary area of applied chemistry, biology and technology. Several process such as freezing, cutting, drying, tempering, bleaching, sterilization, extraction and filtering have been applied efficiency in the food industry. Due to the rapid development of food and peripheral technology, the use of new physical processing or auxiliary processing methods can maintain food inherent nutrients, texture, color, and freshness and also reduce environmental pollution and energy consumption in food processing. Hence, this review paper will study and summarize the effects of green manufacturing process in food industries in term of waste reduction, materials and sustainability manufacturing. In any case, All the food processing equipment must comply with strict standards and regulation, this action will ensure the securing the food quality and safety of food products to consumers.

  4. Information delivery manuals to integrate building product information into design

    DEFF Research Database (Denmark)

    Berard, Ole Bengt; Karlshøj, Jan

    2011-01-01

    Despite continuing BIM progress, professionals in the AEC industry often lack the information they need to perform their work. Although this problem could be alleviated by information systems similar to those in other industries, companies struggle to model processes and information needs...... them in information systems. BIM implies that objects are bearers of information and logic. The present study has three main aims: (1) to explore IDMs capability to capture all four perspectives, (2) to determine whether an IDM’s collaborative methodology is valid for developing standardized processes......, and (3) to ascertain whether IDM’s business rules can support the development of information and logic-bearing BIM objects. The research is based on a case study of re-engineering the bidding process for a design-build project to integrate building product manufacturers, subcontractors...

  5. Sesamin Ameliorates Advanced Glycation End Products-Induced Pancreatic β-Cell Dysfunction and Apoptosis

    Directory of Open Access Journals (Sweden)

    Xiang Kong

    2015-06-01

    Full Text Available Advanced glycation end products (AGEs, the direct modulators of β-cells, have been shown to cause insulin-producing β-cell dysfunction and apoptosis through increase of intracellular reactive oxygen species (ROS production. Sesamin has been demonstrated to possess antioxidative activity. This study was designed to investigate whether sesamin protects against AGEs-evoked β-cell damage via its antioxidant property. The effects of sesamin were examined in C57BL/6J mice and MIN6 cell line. In in vivo studies, mice were intraperitoneally injected with AGEs (120 mg/kg and orally treated with sesamin (160 mg/kg for four weeks. Intraperitoneal glucose tolerance and insulin releasing tests were performed. Insulin content, ROS generation and β-cell apoptosis in pancreatic islets were also measured. In in vitro studies, MIN6 cells were pretreated with sesamin (50 or 100 μM and then exposed to AGEs (200 mg/L for 24 h. Insulin secretion, β-cell death, ROS production as well as expression and activity of NADPH oxidase were determined. Sesamin treatment obviously ameliorated AGE-induced β-cell dysfunction and apoptosis both in vivo and in vitro. These effects were associated with decreased ROS production, down-regulated expression of p67phox and p22phox, and reduced NADPH oxidase activity. These results suggest that sesamin protects β-cells from damage caused by AGEs through suppressing NADPH oxidase-mediated oxidative stress.

  6. A cyclotron isotope production facility designed to maximize production and minimize radiation dose

    International Nuclear Information System (INIS)

    Dickie, W.J.; Stevenson, N.R.; Szlavik, F.F.

    1993-01-01

    Continuing increases in requirements from the nuclear medicine industry for cyclotron isotopes is increasing the demands being put on an aging stock of machines. In addition, with the 1990 recommendations of the ICRP publication in place, strict dose limits will be required and this will have an effect on the way these machines are being operated. Recent advances in cyclotron design combined with lessons learned from two decades of commercial production mean that new facilities can result in a substantial charge on target, low personnel dose, and minimal residual activation. An optimal facility would utilize a well engineered variable energy/high current H - cyclotron design, multiple beam extraction, and individual target caves. Materials would be selected to minimize activation and absorb neutrons. Equipment would be designed to minimize maintenance activities performed in high radiation fields. (orig.)

  7. Cell design concepts for aqueous lithium-oxygen batteries: A model-based assessment

    Science.gov (United States)

    Grübl, Daniel; Bessler, Wolfgang G.

    2015-11-01

    Seven cell design concepts for aqueous (alkaline) lithium-oxygen batteries are investigated using a multi-physics continuum model for predicting cell behavior and performance in terms of the specific energy and specific power. Two different silver-based cathode designs (a gas diffusion electrode and a flooded cathode) and three different separator designs (a porous separator, a stirred separator chamber, and a redox-flow separator) are compared. Cathode and separator thicknesses are varied over a wide range (50 μm-20 mm) in order to identify optimum configurations. All designs show a considerable capacity-rate effect due to spatiotemporally inhomogeneous precipitation of solid discharge product LiOH·H2O. In addition, a cell design with flooded cathode and redox-flow separator including oxygen uptake within the external tank is suggested. For this design, the model predicts specific power up to 33 W/kg and specific energy up to 570 Wh/kg (gravimetric values of discharged cell including all cell components and catholyte except housing and piping).

  8. Elastin-like polypeptides: the power of design for smart cell encapsulation.

    Science.gov (United States)

    Bandiera, Antonella

    2017-01-01

    Cell encapsulation technology is still a challenging issue. Innovative methodologies such as additive manufacturing, and alternative bioprocesses, such as cell therapeutic delivery, where cell encapsulation is a key tool are rapidly gaining importance for their potential in regenerative medicine. Responsive materials such as elastin-based recombinant expression products have features that are particularly attractive for cell encapsulation. They can be designed and tailored to meet desired requirements. Thus, they represent promising candidates for the development of new concept-based materials that can be employed in this field. Areas covered: An overview of the design and employment of elastin-like polypeptides for cell encapsulation is given to outline the state of the art. Special attention is paid to the design of the macromolecule employed as well as to the method of matrix formation and the biological system involved. Expert opinion: As a result of recent progress in regenerative medicine there is a compelling need for materials that provide specific properties and demonstrate defined functional features. Rationally designed materials that may adapt according to applied external stimuli and that are responsive to biological systems, such as elastin-like polypeptides, belong to this class of smart material. A run through the components described to date represents a good starting point for further advancement in this area. Employment of these components in cell encapsulation application will promote its advance toward 'smart cell encapsulation technology'.

  9. Cell design for the DARHT linear induction accelerators

    International Nuclear Information System (INIS)

    Burns, M.; Allison, P.; Earley, L.; Liska, D.; Mockler, C.; Ruhe, J.; Tucker, H.; Walling, L.

    1991-01-01

    The Dual-Axis Radiographic Hydrotest (DARHT) facility will employ two linear induction accelerators to produce intense, bremsstrahlung x- ray pulses for flash radiography. The accelerator cell design for a 3- kA, 16--20 MeV, 60-ns flattop, high-brightness electron beam is presented. The cell is optimized for high-voltage stand-off while also minimizing the its transverse impedance. Measurements of high- voltage and rf characteristics are summarized. 7 refs., 5 figs

  10. Cell culture media impact on drug product solution stability.

    Science.gov (United States)

    Purdie, Jennifer L; Kowle, Ronald L; Langland, Amie L; Patel, Chetan N; Ouyang, Anli; Olson, Donald J

    2016-07-08

    To enable subcutaneous administration of monoclonal antibodies, drug product solutions are often needed at high concentrations. A significant risk associated with high drug product concentrations is an increase in aggregate level over the shelf-life dating period. While much work has been done to understand the impact of drug product formulation on aggregation, there is limited understanding of the link between cell culture process conditions and soluble aggregate growth in drug product. During cell culture process development, soluble aggregates are often measured at harvest using cell-free material purified by Protein A chromatography. In the work reported here, cell culture media components were evaluated with respect to their impact on aggregate levels in high concentration solution drug product during accelerated stability studies. Two components, cysteine and ferric ammonium citrate, were found to impact aggregate growth rates in our current media (version 1) leading to the development of new chemically defined media and concentrated feed formulations. The new version of media and associated concentrated feeds (version 2) were evaluated across four cell lines producing recombinant IgG4 monoclonal antibodies and a bispecific antibody. In all four cell lines, the version 2 media reduced aggregate growth over the course of a 12 week accelerated stability study compared with the version 1 media, although the degree to which aggregate growth decreased was cell line dependent. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:998-1008, 2016. © 2016 American Institute of Chemical Engineers.

  11. Stress analysis for wall structure in mobile hot cell design

    Energy Technology Data Exchange (ETDEWEB)

    Bahrin, Muhammad Hannan, E-mail: hannan@nuclearmalaysia.gov.my; Rahman, Anwar Abdul, E-mail: anwar@nuclearmalaysia.gov.my; Hamzah, Mohd Arif, E-mail: arif@nuclearmalaysia.gov.my; Mamat, Mohd Rizal; Azman, Azraf; Hasan, Hasni [Prototype and Plant Development Centre, Technical Services Division, Malaysian Nuclear Agency (Malaysia)

    2016-01-22

    Malaysian Nuclear Agency is developing a Mobile Hot Cell (MHC) in order to handle and manage Spent High Activity Radioactive Sources (SHARS) such as teletherapy heads and irradiators. At present, there are only two units of MHC in the world, in South Africa and China. Malaysian Mobile Hot cell is developed by Malaysian Nuclear Agency with the assistance of IAEA expert, based on the design of South Africa and China, but with improved features. Stress analysis has been performed on the design in order to fulfil the safety requirement in operation of MHC. This paper discusses the loading analysis effect from the sand to the MHC wall structure.

  12. Design of experiments on production and reaction of point defects

    Science.gov (United States)

    Kiritani, Michio

    Experiments designed by the author for the research of production and reaction of point defects during his stay in eight universities and research institutes for about forty years are reviewed. Two-step aging method elucidated the stability of vacancy cluster nucleus in quenched metals, and the deformation induced vacancies were detected by the aid of quenched-in vacancies. High-voltage electron microscopes were efficiently used as micro-laboratories, especially to do the quantitative electron microscopy to extract fundamental properties of point defects and defect interactions. Fusion neutron irradiation with a neutron source RTNS-II was a unique systematic experiment on defects produced by large collision cascades. Advantage of the use of thin foil samples for neutron irradiation was emphasized. The temperature control in fission reactor irradiation was improved to eliminate the influence of the reactor power. Temperature cycle reactor irradiation became possible, and a multi-section removable rig was constructed for systematic irradiation. The role of freely migrating point defects in microstructure evolution was detected by changing the depth of damage zone with the incidence of self-ions with glancing angles. Finally, the ultra-high-speed plastic deformation gave rise to the anomalous production of vacancies and vacancy clusters, and then to the proposal of plastic deformation of metals without dislocations.

  13. Design of smart functional apparel products for moxa moxibustion

    Science.gov (United States)

    Li, Li; Au, Wai-man; Ding, Feng; Wong, Kwok-shing

    2013-08-01

    Moxa Moxibustion is a common traditional Chinese therapy in which burning Moxa is applied to affected body areas. This method has been employed for thousands of years to achieve certain medical objectives, such as pain relief or antibacterial and anti-inflammatory effects. Its therapeutic effectiveness has been demonstrated successfully both in research and clinical studies. However, this traditional approach may cause undesirable side effects, for example: 1) burning of Moxa produces by-products such as smoke and ash; 2) patients are at risk of being burnt; 3) the active ingredients of the Moxa leaf oil are volatile, odorous, unstable in air and easy to dissipate, and difficult to store and transport; 4) it is inconvenient to operate. These side effects limit its further high-potential and high-value applications. This study is aimed at developing a multi-functional smart textile system that will adopt smart fabrics containing encapsulated Moxa oil integrated with thermally conductive materials to replace the conventional Moxa products. This will efficiently deliver the active ingredients of Moxa to a human body at optimum conditions, i.e., in a precise and controllable way, with maximum convenience and a high level of comfort. Doing so would solve the existing problems mentioned above. Both garment design skill and textile technology will be applied to Moxa Moxibustion textile to enhance the aesthetics and functionality. The smart garment performance will be assessed subjectively in a clinical trial and objectively by a number of instrumental methods.

  14. Fuel Production from Seawater and Fuel Cells Using Seawater.

    Science.gov (United States)

    Fukuzumi, Shunichi; Lee, Yong-Min; Nam, Wonwoo

    2017-11-23

    Seawater is the most abundant resource on our planet and fuel production from seawater has the notable advantage that it would not compete with growing demands for pure water. This Review focuses on the production of fuels from seawater and their direct use in fuel cells. Electrolysis of seawater under appropriate conditions affords hydrogen and dioxygen with 100 % faradaic efficiency without oxidation of chloride. Photoelectrocatalytic production of hydrogen from seawater provides a promising way to produce hydrogen with low cost and high efficiency. Microbial solar cells (MSCs) that use biofilms produced in seawater can generate electricity from sunlight without additional fuel because the products of photosynthesis can be utilized as electrode reactants, whereas the electrode products can be utilized as photosynthetic reactants. Another important source for hydrogen is hydrogen sulfide, which is abundantly found in Black Sea deep water. Hydrogen produced by electrolysis of Black Sea deep water can also be used in hydrogen fuel cells. Production of a fuel and its direct use in a fuel cell has been made possible for the first time by a combination of photocatalytic production of hydrogen peroxide from seawater and dioxygen in the air and its direct use in one-compartment hydrogen peroxide fuel cells to obtain electric power. © 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

  15. Designs and Architectures for the Next Generation of Organic Solar Cells

    Directory of Open Access Journals (Sweden)

    Kang-Shyang Liao

    2010-06-01

    Full Text Available Organic solar cells show great promise as an economically and environmentally friendly technology to utilize solar energy because of their simple fabrication processes and minimal material usage. However, new innovations and breakthroughs are needed for organic solar cell technology to become competitive in the future. This article reviews research efforts and accomplishments focusing on three issues: power conversion efficiency, device stability and processability for mass production, followed by an outlook for optimizing OSC performance through device engineering and new architecture designs to realize next generation organic solar cells.

  16. Audience design affects acoustic reduction via production facilitation.

    Science.gov (United States)

    Arnold, Jennifer E; Kahn, Jason M; Pancani, Giulia C

    2012-06-01

    In this article, we examine the hypothesis that acoustic variation (e.g., reduced vs. prominent forms) results from audience design. Bard et al. (Journal of Memory and Language 42:1-22, 2000) have argued that acoustic prominence is unaffected by the speaker's estimate of addressee knowledge, using paradigms that contrast speaker and addressee knowledge. This question was tested in a novel paradigm, focusing on the effects of addressees' feedback about their understanding of the speaker's intended message. Speakers gave instructions to addressees about where to place objects (e.g., the teapot goes on red). The addressee either anticipated the object, by picking it up before the instruction, or waited for the instruction. For anticipating addressees, speakers began speaking more quickly and pronounced the word the with shorter duration, demonstrating effects of audience design. However, no effects appeared on the head noun (e.g., teapot), as measured by duration, amplitude, and perceived intelligibility. These results are consistent with a mechanism in which evidence about addressee understanding facilitates production processes, as opposed to triggering particular acoustic forms.

  17. Methods for production of proteins in host cells

    Science.gov (United States)

    Donnelly, Mark; Joachimiak, Andrzej

    2004-01-13

    The present invention provides methods for the production of proteins, particularly toxic proteins, in host cells. The invention provides methods which use a fusion protein comprising a chaperonin binding domain in host cells induced or regulated to have increased levels of chaperonin which binds the chaperonin binding domain.

  18. Host cells and methods for production of isobutanol

    Energy Technology Data Exchange (ETDEWEB)

    Anthony, Larry Cameron; He, Hongxian; Huang, Lixuan Lisa; Okeefe, Daniel P.; Kruckeberg, Arthur Leo; Li, Yougen; Maggio-Hall, Lori; McElvain, Jessica; Nelson, Mark J.; Patnaik, Ranjan; Rothman, Steven Cary

    2017-10-17

    Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.

  19. A novel design for solid oxide fuel cell stacks

    Energy Technology Data Exchange (ETDEWEB)

    Al-Qattan, A.M.; Chmielewski, D.J.; Al-Hallaj, S.; Selman, J.R. [Illinois Inst. of Technology, Chicago, IL (United States). Dept. of Chemical and Environmental Engineering

    2004-01-01

    Conventional fuel cell stack designs suffer from severe spatial nonuniformity in both temperature and current density. Such variations are known to create damaging thermal stresses within the stack and thus, impact overall lifespan. In this work, we propose a novel stack design aimed at reducing spatial variations at the source. We propose a mechanism of distributed fuel feed in which the heat generation profile can be influenced directly. Simulation results are presented to illustrate the potential of the proposed scheme. (author)

  20. Optimized microbial cells for production of melatonin and other compounds

    DEFF Research Database (Denmark)

    2017-01-01

    Described herein are recombinant microbial host cells comprising biosynthetic pathways and their use in producing oxidation products and downstream products, e.g., melatonin and related compounds, as well as enzyme variants, nucleic acids, vectors and methods useful for preparing and using...

  1. Production of alkaline protease by Teredinobacter turnirae cells ...

    African Journals Online (AJOL)

    The conditions for immobilizing the new alkaline protease-producing bacteria strain Teredinobacter turnirae by entrapment in calcium alginate gel were investigated. The influence of alginate concentration (20, 25 and 30 g/l) and initial cell loading (ICL) on enzyme production were studied. The production of alkaline ...

  2. Human invariant NKT cell subsets differentially promote differentiation, antibody production, and T cell stimulation by B cells in vitro.

    OpenAIRE

    O'REILLY, VINCENT

    2013-01-01

    PUBLISHED Invariant NK T (iNKT) cells can provide help for B cell activation and Ab production. Because B cells are also capable of cytokine production, Ag presentation, and T cell activation, we hypothesized that iNKT cells will also influence these activities. Furthermore, subsets of iNKT cells based on CD4 and CD8 expression that have distinct functional activities may differentially affect B cell functions. We investigated the effects of coculturing expanded human CD4(+), CD8α(+), and ...

  3. Production of recombinant proteins in suspension-cultured plant cells.

    Science.gov (United States)

    Plasson, Carole; Michel, Rémy; Lienard, David; Saint-Jore-Dupas, Claude; Sourrouille, Christophe; de March, Ghislaine Grenier; Gomord, Véronique

    2009-01-01

    Plants have emerged in the past decade as a suitable alternative to the current production systems for recombinant pharmaceutical proteins and, today their potential for low-cost production of high quality, much safer and biologically active mammalian proteins is largely documented. Among various plant expression systems being explored, genetically modified suspension-cultured plant cells offer a promising system for production of biopharmaceuticals. Indeed, when compared to other plant-based production platforms that have been explored, suspension-cultured plant cells have the advantage of being totally devoid of problems associated with the vagaries of weather, pest, soil and gene flow in the environment. Because of short growth cycles, the timescale needed for the production of recombinant proteins in plant cell culture can be counted in days or weeks after transformation compared to months needed for the production in transgenic plants. Moreover, recovery and purification of recombinant proteins from plant biomass is an expensive and technically challenging business that may amount to 80-94% of the final product cost. One additional advantage of plant cell culture is that the recombinant protein fused with a signal sequence can be expressed and secreted into the culture medium, and therefore recovered and purified in the absence of large quantities of contaminating proteins. Consequently, the downstream processing of proteins extracted from plant cell culture medium is less expensive, which may/does balance the higher costs of fermentation. When needed for clinical use, recombinant proteins are easily produced in suspension-cultured plant cells under certified, controllable and sterile conditions that offer improved safety and provide advantages for good manufacturing practices and regulatory compliance. In this chapter, we present basic protocols for rapid generation of transgenic suspension-cultured cells of Nicotiana tabacum, Oriza sativa and Arabidopis

  4. Honeybee product therapeutic as stem cells homing for ovary failure

    Directory of Open Access Journals (Sweden)

    Erma Safitri

    2016-11-01

    Full Text Available Aim: Complexity of the method of isolation, cultivation in vitro and the expensive cost of transplantation process of stem cells, it would require an innovation to homing and differentiation of stem cells and increase folliculogenesis. The stem cells homing was achieved through the provision of food or beverages derived from natural materials like honeybee product. Through honeybee product, there will be homing of stem cells and accompany with the sources from the body itself will take place in regeneration of the ovary. Materials and Methods: Female rats model of degenerative ovary was obtained through food fasting but still have drinking water for 5 days. It caused malnutrition and damage of the ovarian tissue. The administration of 50% honeybee product (T1 was performed for 10 consecutive days, while the positive control group (T0+ was fasted and not given honeybee product and the negative control (T0− not fasted and without honeybee product. Observations were taken for homing of stem cells, raised of folliculogenesis, differentiation of stem cells, and regeneration of the ovarian tissue using routine H&E staining. Results: Homing of stem cells shown the vascular endothelial growth factor and granulocyte colony-stimulating factor expression; enhancement of folliculogenesis was indicated by an increase of follicle dee Graaf count; enhancement of differentiation of stem cells was indicated by growth differentiation factor-9 expression; and regeneration of ovarian tissue indicated by intact ovarian tissue with growing follicles. Conclusion: Honeybee product can be induced endogenous stem cells in regeneration of ovary failure due to malnutrition.

  5. Life Time Performance Characterization of Solid Oxide Electrolysis Cells for Hydrogen Production

    DEFF Research Database (Denmark)

    Sun, Xiufu; Chen, Ming; Liu, Yi-Lin

    2015-01-01

    Solid oxide electrolysis cells (SOECs) offer a promising technological solution for efficient energy conversion and production of hydrogen or syngas. The commercialization of the SOEC technology can be promoted if SOECs can be operated at high current density with stable performance over ~5 years...... - 3 years (continuous operation, setting 1.5 V as the upper voltage defining “end of life”). The results provide technological input to future design of electrolysis plants for hydrogen production. © 2015 ECS - The Electrochemical Society...

  6. Design of a Fuel Cell Hybrid Electric Vehicle Drive System

    DEFF Research Database (Denmark)

    Schaltz, Erik

    Fuel cells achieve more and more attention due to their potential of replacing the traditional internal combustion engine (ICE) used in the area of transportation. In this PhD thesis a fuel cell shaft power pack (FCSPP) is designed and implemented in a small truck. The FCSPP replaces the original...... supply system of the truck which was powered by a lead-acid battery package. The FCSPP includes fuel storage, a fuel cell system, an energy storage device, power electronics, an electric machine, and the necessary control. The FCSPP therefore converts the energy of the fuel to a shaft torque and speed...... of the electric machine. In the thesis the High Temperature Proton Exchange Membrane Fuel Cell (HTPEMFC) is used as it has promising properties for being supplied by reformed methanol, instead of pure hydrogen, which is more practical feasible. It takes approximately 6 minutes before the fuel cell is ready...

  7. Emerging Semitransparent Solar Cells: Materials and Device Design.

    Science.gov (United States)

    Tai, Qidong; Yan, Feng

    2017-09-01

    Semitransparent solar cells can provide not only efficient power-generation but also appealing images and show promising applications in building integrated photovoltaics, wearable electronics, photovoltaic vehicles and so forth in the future. Such devices have been successfully realized by incorporating transparent electrodes in new generation low-cost solar cells, including organic solar cells (OSCs), dye-sensitized solar cells (DSCs) and organometal halide perovskite solar cells (PSCs). In this review, the advances in the preparation of semitransparent OSCs, DSCs, and PSCs are summarized, focusing on the top transparent electrode materials and device designs, which are all crucial to the performance of these devices. Techniques for optimizing the efficiency, color and transparency of the devices are addressed in detail. Finally, a summary of the research field and an outlook into the future development in this area are provided. © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  8. Experimental Design to Evaluate Directed Adaptive Mutation in Mammalian Cells

    Science.gov (United States)

    Chiaro, Christopher R; May, Tobias

    2014-01-01

    Background We describe the experimental design for a methodological approach to determine whether directed adaptive mutation occurs in mammalian cells. Identification of directed adaptive mutation would have profound practical significance for a wide variety of biomedical problems, including disease development and resistance to treatment. In adaptive mutation, the genetic or epigenetic change is not random; instead, the presence and type of selection influences the frequency and character of the mutation event. Adaptive mutation can contribute to the evolution of microbial pathogenesis, cancer, and drug resistance, and may become a focus of novel therapeutic interventions. Objective Our experimental approach was designed to distinguish between 3 types of mutation: (1) random mutations that are independent of selective pressure, (2) undirected adaptive mutations that arise when selective pressure induces a general increase in the mutation rate, and (3) directed adaptive mutations that arise when selective pressure induces targeted mutations that specifically influence the adaptive response. The purpose of this report is to introduce an experimental design and describe limited pilot experiment data (not to describe a complete set of experiments); hence, it is an early report. Methods An experimental design based on immortalization of mouse embryonic fibroblast cells is presented that links clonal cell growth to reversal of an inactivating polyadenylation site mutation. Thus, cells exhibit growth only in the presence of both the countermutation and an inducing agent (doxycycline). The type and frequency of mutation in the presence or absence of doxycycline will be evaluated. Additional experimental approaches would determine whether the cells exhibit a generalized increase in mutation rate and/or whether the cells show altered expression of error-prone DNA polymerases or of mismatch repair proteins. Results We performed the initial stages of characterizing our system

  9. An in silico platform for the design of heterologous pathways in nonnative metabolite production

    Directory of Open Access Journals (Sweden)

    Chatsurachai Sunisa

    2012-05-01

    Full Text Available Abstract Background Microorganisms are used as cell factories to produce valuable compounds in pharmaceuticals, biofuels, and other industrial processes. Incorporating heterologous metabolic pathways into well-characterized hosts is a major strategy for obtaining these target metabolites and improving productivity. However, selecting appropriate heterologous metabolic pathways for a host microorganism remains difficult owing to the complexity of metabolic networks. Hence, metabolic network design could benefit greatly from the availability of an in silico platform for heterologous pathway searching. Results We developed an algorithm for finding feasible heterologous pathways by which nonnative target metabolites are produced by host microorganisms, using Escherichia coli, Corynebacterium glutamicum, and Saccharomyces cerevisiae as templates. Using this algorithm, we screened heterologous pathways for the production of all possible nonnative target metabolites contained within databases. We then assessed the feasibility of the target productions using flux balance analysis, by which we could identify target metabolites associated with maximum cellular growth rate. Conclusions This in silico platform, designed for targeted searching of heterologous metabolic reactions, provides essential information for cell factory improvement.

  10. Effect of media components on cell growth and bacterial cellulose production from Acetobacter aceti MTCC 2623.

    Science.gov (United States)

    Dayal, Manmeet Singh; Goswami, Navendu; Sahai, Anshuman; Jain, Vibhor; Mathur, Garima; Mathur, Ashwani

    2013-04-15

    Acetobacter aceti MTCC 2623 was studied as an alternative microbial source for bacterial cellulose (BC) production. Effect of media components on cell growth rate, BC production and cellulose characteristics were studied. FTIR results showed significant variations in cellulose characteristics produced by A. aceti in different media. Results have shown the role of fermentation time on crystallinity ratio of BC in different media. Further, effect of six different media components on cell growth and BC production was studied using fractional factorial design. Citric acid was found to be the most significant media component for cell growth rate (95% confidence level, R(2)=0.95). However, direct role of these parameters on cellulose production was not established (p-value>0.05). Copyright © 2013 Elsevier Ltd. All rights reserved.

  11. Automation of cells of radiopharmaceuticals production

    International Nuclear Information System (INIS)

    Negrini, Aguinaldo Donizete

    2010-01-01

    The 67 Ga is an important radiopharmaceutical used to identify inflammatory processes in chronic illnesses, diagnosis by image of tumors in soft tissues and the possibility to evaluate the result for therapeutic intervention. In the present work a module of 67 Ga processing was developed with the objective to reduce the interventions in the hot cell, in order to avoid oxidation caused by metallic materials, and consuming in hoses of the peristaltic pumps, that release residues that blocked the valves used in the process. With materials such as: acrylic, PVC, PEEK e teflon and they are used vacuum as method (way) of fluid transferences instead of peristaltic pump in the majority of the procedures, with this improvements the system can make shorter the lengths of transference hoses, increasing the yield in the process with less interventions for maintenance and time exposure of the workers, guaranteeing the quality and reducing the time of the processing. using a mobile system for displacement of the processing module making in the cleanness and maintenance of the cell that works with radioactive material. Reducing the time of exposure dose of the workers in compliance with RDC-17 of ANVISA, which ruling the Good Manufacturing Practice Procedures. (author)

  12. Improved performance design of gallium arsenide solar cells for space

    Science.gov (United States)

    Parekh, R. H.; Barnett, A. M.

    1984-01-01

    An improved design, shallow junction heteroface, n-p, gallium arsenide solar cell for space applications is reported, with a predicted AM0 efficiency in the 21.9 to 23.0 percent range. The optimized n-p structure, while slightly more efficient, has the added advantage of being less susceptible to radiation-induced degradation by virtue of this thin top junction layer. Detailed spectral response curves and an analysis of the loss mechanisms are reported. The details of the design are readily measurable. The optimized designs were reached by quantifying the dominant loss mechanisms and then minimizing them by using computer simulations.

  13. High-yield secretion of recombinant proteins expressed in tobacco cell culture with a designer glycopeptide tag: Process development.

    Science.gov (United States)

    Zhang, Ningning; Gonzalez, Maria; Savary, Brett; Xu, Jianfeng

    2016-03-01

    Low-yield protein production remains the most significant economic hurdle with plant cell culture technology. Fusions of recombinant proteins with hydroxyproline-O-glycosylated designer glycopeptide tags have consistently boosted secreted protein yields. This prompted us to study the process development of this technology aiming to achieve productivity levels necessary for commercial viability. We used a tobacco BY-2 cell culture expressing EGFP as fusion with a glycopeptide tag comprised of 32 repeat of "Ser-Pro" dipeptide, or (SP)32 , to study cell growth and protein secretion, culture scale-up, and establishment of perfusion cultures for continuous production. The BY-2 cells accumulated low levels of cell biomass (~7.5 g DW/L) in Schenk & Hildebrandt medium, but secreted high yields of (SP)32 -tagged EGFP (125 mg/L). Protein productivity of the cell culture has been stable for 6.0 years. The BY-2 cells cultured in a 5-L bioreactor similarly produced high secreted protein yield at 131 mg/L. Successful operation of a cell perfusion culture for 30 days was achieved under the perfusion rate of 0.25 and 0.5 day(-1) , generating a protein volumetric productivity of 17.6 and 28.9 mg/day/L, respectively. This research demonstrates the great potential of the designer glycopeptide technology for use in commercial production of valuable proteins with plant cell cultures. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

  14. Impact of cell culture on recombinant monoclonal antibody product heterogeneity.

    Science.gov (United States)

    Liu, Hongcheng; Nowak, Christine; Shao, Mei; Ponniah, Gomathinayagam; Neill, Alyssa

    2016-09-01

    Recombinant monoclonal antibodies are commonly expressed in mammalian cell culture and purified by several steps of filtration and chromatography. The resulting high purity bulk drug substance still contains product variants differing in properties such as charge and size. Posttranslational modifications and degradations occurring during cell culture are the major sources of heterogeneity in bulk drug substance of recombinant monoclonal antibodies. The focus of the current review is the impact of cell culture conditions on the types and levels of various modifications and degradations of recombinant monoclonal antibodies. Understanding the relationship between cell culture and product variants can help to make consistently safe and efficacious products. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1103-1112, 2016. © 2016 American Institute of Chemical Engineers.

  15. Designing Cost-Competitive Technology Products through Cost Management

    NARCIS (Netherlands)

    Davila, T.; Wouters, Marc

    2004-01-01

    SYNOPSIS: As manufacturing innovations spread throughout leading organizations, product development becomes a more important source of competitive advantage. Within product development, cost management receives increasing attention. To date, cost management in new product development focuses

  16. Fuel Cell Car Design Project for Freshman Engineering Courses

    Science.gov (United States)

    Duke, Steve R.; Davis, Virginia A.

    2014-01-01

    In the Samuel Ginn College of Engineering at Auburn University, we have integrated a semester long design project based on a toy fuel cell car into our freshman "Introduction to Chemical Engineering Class." The project provides the students a basic foundation in chemical reactions, energy, and dimensional analysis that facilitates…

  17. Review scaffold design and stem cells for tooth regeneration

    Directory of Open Access Journals (Sweden)

    Li Zhang

    2013-02-01

    Full Text Available Current dental treatments for the missing teeth depend largely on dentures and implants crowned with prosthetic caps to restore some functionality of the teeth. However, these devices cannot mimic the biological teeth, do not remodel and they have poor integration with the host. The concept of tissue engineering is based on that fact that by cultivating postnatal dental stem cells (DSCs on a well-designed bioengineered three dimensional scaffold, it is possible to regenerate tooth organogenesis. To date, a range of biomaterial scaffolds with different sources of cells have been proposed to regenerate substitutes to the natural extracellular matrix (ECM analogs. The design of scaffold is critical as it should be capable of supporting cell attachment and proliferation and has the appropriate mechanical properties. Moreover, there are a number of parameters that must be examined in constructing the scaffold, including porosity, the mechanical integrity and effect of surface morphology on cell adhesion and proliferation. In this paper a brief review of literature is presented together with a discussion on the future directions and the challenges ahead in the areas of periodontal dental stem cells DSCs and the scaffold design and manufacturing techniques that are of particular significant for tooth tissue engineering.

  18. Recombinant host cells and media for ethanol production

    Energy Technology Data Exchange (ETDEWEB)

    Wood, Brent E; Ingram, Lonnie O; Yomano, Lorraine P; York, Sean W

    2014-02-18

    Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium. Preferred systems are suitable for efficient ethanol production by simultaneous saccharification and fermentation (SSF) using lignocellulose as an oligosaccharide source. The invention also provides novel isolated polynucleotide sequences, polypeptide sequences, vectors and antibodies.

  19. Symbolic meaning integration in design and its influence on product and brand evaluation

    NARCIS (Netherlands)

    van Rompay, Thomas Johannes Lucas; Pruyn, Adriaan T.H.; Tieke, Peter

    2009-01-01

    Previous research and theorizing in product design and consumer psychology testifies to the importance of congruence among symbolic meanings connoted through elements in visual communications such as advertisements, product appearance, and product packaging. However, understanding of the processes

  20. A METHOD OF IMPROVING THE PRODUCTION OF BIOMASS OR A DESIRED PRODUCT FROM A CELL

    DEFF Research Database (Denmark)

    1998-01-01

    the F¿1? ATPase or portions thereof is expressed, may be selected from prokaryotes and eukaryotes. In particular the DNA encoding F¿1? or a portion thereof may be derived from bacteria and eukaryotic microorganisms such as yeasts, other fungi and cell lines of higher organisms and be selected from......The production of biomass or a desired product from a cell can be improved by inducing conversion of ATP to ADP without primary effects on other cellular metabolites or functions which is achieved by expressing an uncoupled ATPase activity in said cell and incubating the cell with a suitable...... substrate to produce said biomass or product. This is conveniently done by expressing in said cell the soluble part (F¿1?) of the membrane bound (F¿0?F¿1? type) H?+¿-ATPase or a portion of F¿1? exhibiting ATPase activity. The organism from which the F¿1? ATPase or portions thereof is derived, or in which...

  1. A practical approach to product design for future worlds using scenario-development

    NARCIS (Netherlands)

    Eggink, Wouter; Reinders, Angelina H.M.E.; van der Meulen, Barend; Clarke, A.

    2009-01-01

    The focus of consumer product design is shifting from primarily offering functionality, towards experience and emotion driven product characteristics [1]. At the same time the functioning of products is more and more defined in its social context. Product designers can play a major role in

  2. Influences of three-dimensional printing to product innovation design thinking

    Directory of Open Access Journals (Sweden)

    Liu Ling

    2017-01-01

    Full Text Available The increasing demand of individuation brings new challenges to traditional product design. This paper studies and analyzes three-dimensional print technology promotes the future development of product innovation design. The advantages of 3D printing equipment can help creative products enterprises shorten product manufacturing period and make them powerful in competition.

  3. IFMIF high flux test module and test cell-Design and design validation

    Energy Technology Data Exchange (ETDEWEB)

    Heinzel, V. [Institute for Reactor Safety, Research Centre of Karlsruhe, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen (Germany)], E-mail: heinzel@irs.fzk.de; Arbeiter, F.; Dolensky, B.; Fischer, U.; Gordeev, S.; Lang, K.-H.; Leichtle, D. [Institute for Reactor Safety, Research Centre of Karlsruhe, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen (Germany); Moeslang, A. [Institute for Material Research I, Research Centre of Karlsruhe, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen (Germany); Simakov, S.P.; Slobodchuk, V.; Stratmanns, E. [Institute for Reactor Safety, Research Centre of Karlsruhe, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen (Germany); Vladimirov, P. [Institute for Material Research I, Research Centre of Karlsruhe, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen (Germany)

    2007-10-15

    The present paper summarizes work devoted to design and design validation of the HFTM and improvements obtained by neutronics, thermohydraulic and mechanical analyses. The HFTM design requirements and its main features will be described. Remarkable progress has been achieved in the development of appropriate computational tools, data and methods for IFMIF neutronics and activation analysis. Similarly, the thermohydraulic simulations have been validated against experimental data obtained in the ITHEX facility to test turbulence models of gas flow in narrow channels. Iterative analyses lead to several design optimizations of container and helium supply. Crucial manufacturing techniques for the parts of the HFTM have been tested extensively to assure a reliable and reproducible manufacturing route for the final procurement of the HFTM. Finally, the integrated design of the Target and Test Cell has been initiated with contributions from several international design teams.

  4. Contending stigma in product design : Using insights from social psychology as a stepping stone for design strategies

    NARCIS (Netherlands)

    Vaes, K.R.V.; Stappers, P.J.; Standaert, A.; Desager, K.

    2012-01-01

    Assistive, protective or medical products that are visibly worn or used in proximity to the human body can have an emotional impact on their users and bystanders. In his design effort it is important for a designer to be mindful of the potential stigma a product might elicit. The identity threat

  5. Innovation in product design as a factor for reducing logistic and production costs

    Directory of Open Access Journals (Sweden)

    Luciana Fighera Marzall

    2016-03-01

    Full Text Available Innovation has been considered of fundamental importance for organizations competing in dynamic environments, which along with cost management has been shown as an organizational strategy that allows the company to measure its results and implement measures that impact on the organization's performance as a whole. This paper, carried out in a metal mechanical industry of a telecommunications engineering branch, aims to analyse cost reduction in logistics raw materials areas through innovation in the product design, which are antennas and towers for telephone networks. In terms of methodology, this study presents itself as a qualitative and quantitative research-action, which from the link between theory and practice allows for intervention in the middle of the researchers studied. As a result, an innovative project was developed, which allowed for the reduction costs of production by 68% and logistics by 32%. Despite the significant decrease in costs, the quality of manufactured products not declined and customer needs were met, thus making the company more competitive against competitors. Not widespread in the literature, this analysis of the impact on logistics and production costs due to improvements in the project is the main contribution theoretical of this paper.

  6. Accelerated and Rational Design of Improved CHO Cell Factories

    DEFF Research Database (Denmark)

    Grav, Lise Marie

    . This allows us to i) facilitate improved studies of gene functions, ii) remove cellular traits that are disadvantageous to protein production, iii) control and predict the level and stability of protein expression, and iv) design and engineer new cellular functions. Overall, the results of this thesis...

  7. Taking control of charge transfer : strategic design for solar cells

    NARCIS (Netherlands)

    Monti, Adriano

    2015-01-01

    The thesis is focused on the investigation of the electron transfer mechanisms leading to solar fuel production and to the identification of engineering principles that can be used to design materials able to improve charge separation. Molecular systems composed of three or more subunits arranged

  8. H2 PRODUCTION AND FUEL CELLS.

    Energy Technology Data Exchange (ETDEWEB)

    WANG, X.; RODRIGUEZ, J.A.

    2006-06-30

    Oxide nanosystems play a key role as components of catalysts used for the production of H{sub 2} via the steam reforming or the partial oxidation of hydrocarbons, and for the water-gas shift reaction. The behavior seen for Cu-ceria and Au-ceria WGS catalysts indicates that the oxide is much more than a simple support. The special chemical properties of the oxide nanoparticles (defect rich, high mobility of oxygen) favor interactions with the reactants or other catalyst components. More in-situ characterization and mechanistic studies are necessary for the optimization of these nanocatalysts. The use of oxide nanomaterials for the fabrication of PEMFCs and SOFCs can lead to devices with a high practical impact. One objective is to build electrodes with low cost conducting oxide nanoarrays. The electron and oxygen-ion conducting capabilities of many oxides improve when going from the bulk to the nanoscale. Furthermore, one can get a more homogeneous surface morphology and an increase of the effective reaction area. Much more fundamental and practical research needs to be done in this area.

  9. Optimized designs and materials for nanostructure based solar cells

    Science.gov (United States)

    Shao, Qinghui

    Nanostructure-based solar cells are attracting significant attention as possible candidates for drastic improvement in photovoltaic (PV) energy conversion efficiency. Although such solar cells are expected to be more expensive there is growing need for the efficient and light-weight solar cells in aero-space and related industries. In this dissertation I present results of the theoretical, computational and experimental investigation of novel designs for quantum dot superlattice (QDS) based PV elements and advanced materials for transparent solar cells. In the first part of the dissertation I describe possible implementation of the intermediate-band (IB) solar cells with QDS. The IB cells were predicted to have PV efficiency exceeding the Shockley-Queisser limit for a single junction cell. The parameters of QDS structure have to be carefully tuned to achieve the desired charge carrier dispersion required for the IB operation. The first-principles models were used to calculate the electrical properties and light absorption in QDS. This approach allowed me to determine the dimensions of QDS for inducing the mini-band which plays the role of the IB. Using the detailed balance theory it was determined that the upper-bound PV efficiency of such IB solar cells can be as high as ˜51%. The required QDS dimensions on the basis of InAsN/GaAsSb are technologically challenging but feasible: ˜2-6 nm. Using the developed simulation tools I proposed several possible designs of QDS solar cells including one, which combined the benefits of the IB concept and the advanced tandem cell design. The second part of the dissertation presents a study of graphene layers as transparent electrodes for the PV cells. The graphene layers were mechanically exfoliated from bulk graphite and characterized with micro-Raman spectroscopy. It was found that graphene electrodes have good electrical conductivity, which reveals unusual temperature dependence beneficial for the proposed application. The

  10. Conceptual design report -- Gasification Product Improvement Facility (GPIF)

    Energy Technology Data Exchange (ETDEWEB)

    Sadowski, R.S.; Skinner, W.H.; House, L.S.; Duck, R.R. [CRS Sirrine Engineers, Inc., Greenville, SC (United States); Lisauskas, R.A.; Dixit, V.J. [Riley Stoker Corp., Worcester, MA (United States); Morgan, M.E.; Johnson, S.A. [PSI Technology Co., Andover, MA (United States). PowerServe Div.; Boni, A.A. [PSI-Environmental Instruments Corp., Andover, MA (United States)

    1994-09-01

    The problems heretofore with coal gasification and IGCC concepts have been their high cost and historical poor performance of fixed-bed gasifiers, particularly on caking coals. The Gasification Product Improvement Facility (GPIF) project is being developed to solve these problems through the development of a novel coal gasification invention which incorporates pyrolysis (carbonization) with gasification (fixed-bed). It employs a pyrolyzer (carbonizer) to avoid sticky coal agglomeration caused in the conventional process of gradually heating coal through the 400 F to 900 F range. In so doing, the coal is rapidly heated sufficiently such that the coal tar exists in gaseous form rather than as a liquid. Gaseous tars are then thermally cracked prior to the completion of the gasification process. During the subsequent endothermic gasification reactions, volatilized alkali can become chemically bound to aluminosilicates in (or added to) the ash. To reduce NH{sub 3} and HCN from fuel born nitrogen, steam injection is minimized, and residual nitrogen compounds are partially chemically reduced in the cracking stage in the upper gasifier region. Assuming testing confirms successful deployment of all these integrated processes, future IGCC applications will be much simplified, require significantly less mechanical components, and will likely achieve the $1,000/kWe commercialized system cost goal of the GPIF project. This report describes the process and its operation, design of the plant and equipment, site requirements, and the cost and schedule. 23 refs., 45 figs., 23 tabs.

  11. High specific energy, high capacity nickel-hydrogen cell design

    Science.gov (United States)

    Wheeler, James R.

    1993-01-01

    A 3.5 inch rabbit-ear-terminal nickel-hydrogen cell was designed and tested to deliver high capacity at steady discharge rates up to and including a C rate. Its specific energy yield of 60.6 wh/kg is believed to be the highest yet achieved in a slurry-process nickel-hydrogen cell, and its 10 C capacity of 113.9 AH the highest capacity yet of any type in a 3.5 inch diameter size. The cell also demonstrated a pulse capability of 180 amps for 20 seconds. Specific cell parameters and performance are described. Also covered is an episode of capacity fading due to electrode swelling and its successful recovery by means of additional activation procedures.

  12. Curcumin inhibits endometriosis endometrial cells by reducing estradiol production.

    Science.gov (United States)

    Zhang, Ying; Cao, Hong; Yu, Zheng; Peng, Hai-Ying; Zhang, Chang-Jun

    2013-05-01

    Endometriosis is a complex estrogen-dependent disease that is defined as the presence of endometrial gland and stroma outside the uterine cavity. Although the exact mechanism for the development of endometriosis remains unclear, there is a large body of research data and circumstantial evidence that suggests a crucial role of estrogen in the establishment and maintenance of this disease. This study is an attempt to assess the effect of curcumin on inhibiting endometriosis endometrial cells and to investigate whether such an effect is mediated by reducing estradiol production. Endometriotic stromal cells, normal endometrial stromal cells, endometriotic epithelial cells and normal endometrial epithelial cells were isolated and cultured. E2 value of cells and the effect of curcumin on cell proliferation were evaluated. Finally, effect of curcumin on E2 assay was detected. Electrochemiluminescence immunoassay results showed that E2 value of endometriotic epithelial cells was higher than the endometriotic stromal cells (p=0.037), while the expression of E2 in normal endometrial stromal and epithelial cells was extremely low. WST-8 result showed, compared with endometrial stromal cells, ectopic endometriotic stromal cells had a higher growth rate. After intervene with curcumin (10μmol/L, 30μmol/L and 50μmol/L) for 0-96h, the number of endometriotic stromal cells was reduced and cells growth slowed, compared with 0μmol/L group. Compared with 0μmol/L group, E2 level was lower after treatment with curcumin, especially in 30μmol/L and 50μmol/L group. In summary, in this study we found that E2 is important in ectopic endometrium, and epithelial cell is in dominant position with E2 secretion. Curcumin was able to suppress the proliferation of endometrial cells by reducing the E2 value.

  13. Influence of scaffold design on 3D printed cell constructs.

    Science.gov (United States)

    Souness, Auryn; Zamboni, Fernanda; Walker, Gavin M; Collins, Maurice N

    2018-02-01

    Additive manufacturing is currently receiving significant attention in the field of tissue engineering and biomaterial science. The development of precise, affordable 3D printing technologies has provided a new platform for novel research to be undertaken in 3D scaffold design and fabrication. In the past, a number of 3D scaffold designs have been fabricated to investigate the potential of a 3D printed scaffold as a construct which could support cellular life. These studies have shown promising results; however, few studies have utilized a low-cost desktop 3D printing technology as a potential rapid manufacturing route for different scaffold designs. Here six scaffold designs were manufactured using a Fused deposition modeling, a "bottom-up" solid freeform fabrication approach, to determine optimal scaffold architecture for three-dimensional cell growth. The scaffolds, produced from PLA, are coated using pullulan and hyaluronic acid to assess the coating influence on cell proliferation and metabolic rate. Scaffolds are characterized both pre- and postprocessing using water uptake analysis, mechanical testing, and morphological evaluation to study the inter-relationships between the printing process, scaffold design, and scaffold properties. It was found that there were key differences between each scaffold design in terms of porosity, diffusivity, swellability, and compressive strength. An optimal design was chosen based on these physical measurements which were then weighted in accordance to design importance based on literature and utilizing a design matrix technique. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 533-545, 2018. © 2017 Wiley Periodicals, Inc.

  14. Host cell proteins in biotechnology-derived products: A risk assessment framework.

    Science.gov (United States)

    de Zafra, Christina L Zuch; Quarmby, Valerie; Francissen, Kathleen; Vanderlaan, Martin; Zhu-Shimoni, Judith

    2015-11-01

    To manufacture biotechnology products, mammalian or bacterial cells are engineered for the production of recombinant therapeutic human proteins including monoclonal antibodies. Host cells synthesize an entire repertoire of proteins which are essential for their own function and survival. Biotechnology manufacturing processes are designed to produce recombinant therapeutics with a very high degree of purity. While there is typically a low residual level of host cell protein in the final drug product, under some circumstances a host cell protein(s) may copurify with the therapeutic protein and, if it is not detected and removed, it may become an unintended component of the final product. The purpose of this article is to enumerate and discuss factors to be considered in an assessment of risk of residual host cell protein(s) detected and identified in the drug product. The consideration of these factors and their relative ranking will lead to an overall risk assessment that informs decision-making around how to control the levels of host cell proteins. © 2015 Wiley Periodicals, Inc.

  15. Verification of product design using regulation knowledge base and Web services

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Ik June [KAERI, Daejeon (Korea, Republic of); Lee, Jae Chul; Mun Du Hwan [Kyungpook National University, Daegu (Korea, Republic of); Kim, Byung Chul [Dong-A University, Busan (Korea, Republic of); Hwang, Jin Sang [PartDB Co., Ltd., Daejeom (Korea, Republic of); Lim, Chae Ho [Korea Institute of Industrial Technology, Incheon (Korea, Republic of)

    2015-11-15

    Since product regulations contain important rules or codes that manufacturers must follow, automatic verification of product design with the regulations related to a product is necessary. For this, this study presents a new method for the verification of product design using regulation knowledge base and Web services. Regulation knowledge base consisting of product ontology and rules was built with a hybrid technique combining ontology and programming languages. Web service for design verification was developed ensuring the flexible extension of knowledge base. By virtue of two technical features, design verification is served to various products while the change of system architecture is minimized.

  16. Verification of product design using regulation knowledge base and Web services

    International Nuclear Information System (INIS)

    Kim, Ik June; Lee, Jae Chul; Mun Du Hwan; Kim, Byung Chul; Hwang, Jin Sang; Lim, Chae Ho

    2015-01-01

    Since product regulations contain important rules or codes that manufacturers must follow, automatic verification of product design with the regulations related to a product is necessary. For this, this study presents a new method for the verification of product design using regulation knowledge base and Web services. Regulation knowledge base consisting of product ontology and rules was built with a hybrid technique combining ontology and programming languages. Web service for design verification was developed ensuring the flexible extension of knowledge base. By virtue of two technical features, design verification is served to various products while the change of system architecture is minimized.

  17. New developments in Nickel-Hydrogen Dependent Pressure Vessel (DPV) cell and battery design

    Science.gov (United States)

    Caldwell, Dwight B.; Fox, Chris L.; Miller, Lee E.

    1997-01-01

    The Dependent Pressure Vessel (DPV) Nickel-Hydrogen (NiH2) design is being developed by Eagle-Picher Industries, Inc. (EPI) as an advanced battery for military and commercial, aerospace and terrestrial applications. The DPV cell design offers high specific energy, energy density and reduced cost, while retaining the established Individual Pressure Vessel (IPV) technology flight heritage and database. This advanced DPV design also offers a more efficient mechanical, electrical and thermal cell and battery configuration and a reduced parts count. The DPV battery design promotes compact, minimum volume packaging and weight efficiency, and delivers significant cost and weight savings while providing minimal design risks. This presentation will discuss new DPV design concepts and production features and present test data from existing development cells. The DPV combines the unique features and significant advantages of NiH2 electrochemistry with the simplicity and extensive design heritage of the NiCd battery system to create a power source ideal for military and commercial terrestrial projects as well as traditional aerospace applications.

  18. In silico Design of "Un-Natural" Natural Products

    Directory of Open Access Journals (Sweden)

    Zucko; J. ...(et al.

    2008-05-01

    ClustScan, which are under development to model these processes in silico. The heart of CompGen is a specially structured database, based on BioSQL v1.29, which connects the biosynthetic order of synthase/synthetase enzymes to the sequences of the component polypeptides. The additional linkage to the gene sequences allows the integration of DNA sequence with product structure. The database contains sequences of the well-characterised PKS/NRPS clusters, and non-annotated sequenced clusters whose structure and functionis yet unknown, to act as building blocks for the production of novel products. It is easy to add custom sequences to the database and to annotate them by the use of propriety protein profiles designed by Pfam database and HMMER. One function of the program is the ability to generate virtual recombinants between clusters. This can be done using a recombination model (with optional parameters to predict sites for homologous recombination or by user defined recombination sites (e.g. to model in vitro genetic manipulation such as module replacement. The program predicts the linear polyketide structure of the resulting "un-natural" natural products with a chemical description using isomeric SMILES. Molecular modelling of the subsequent spontaneous cyclisation process produces structures for a virtual compound database for further molecular modelling studies using PASS and CDD technology. An optional "reverse genetics" module analyses a given chemical structure to see if it could be produced by a novel PKS/NRPS synthesis cluster and suggests the DNA sequence of a suitable cluster based on building blocks derived from clusters contained in the database.Overall, the CompGen allows in silico generation of the database of novel "un-natural" natural chemical compounds that can be used for in silico screening using PASS or CDD technology. The other integral generic program package, ClustScan, will recognise and annotate new gene clusters from microbial genome

  19. Photonic Nanostructures Design and Optimization for Solar Cell Application

    Directory of Open Access Journals (Sweden)

    Qian Liu

    2015-08-01

    Full Text Available In this paper, a semiconducting photonic nanostructure capable of wide range absorption and tunable optical resonance has been designed with a proposed theoretical optimization model. The design consists of ZnO/CdS core-shell nanowire arrays as well as multilayer thin films that act to absorb incident electromagnetic (EM waves over a broad frequency range. Theoretical, as well as numerical, studies of the nanostructure inside a solar cell plate have been conducted in order to validate the proposed microstructural design. Excellent energy absorption rates of EM waves have been achieved in the high frequency range by using the optical resonance of the nanowire array. By combining multilayer thin film with the core-shell nanowire in the unit cell of a photonic solar cell, a broadband high absorption has been achieved. Moreover, the geometry of the proposed photonic nanostructure is obtained through the implementation of a genetic algorithm. This avoids local minima and an optimized absorption rate of ~90% over the frequency range of 300 to 750 THz has been obtained in the solar cell.

  20. Improving Labor Productivity and Labor Elasticity at Multiproduct Japanese Cuisine Restaurant Introducing Cell-Production System

    OpenAIRE

    Shimamura, Takeshi; Takenaka, Takeshi; Ohura, Syuichi

    2013-01-01

    Part III: Sustainable Services; International audience; This study examined improvement of labor productivity and elasticity of labor hour on sales of a multiproduct Japanese cuisine restaurant. Conventionally, multiproduct restaurant operations include a line production system in the kitchen. Japanese chefs are assumed to be low-skilled workers with staff members supported by someone. A cell production system is introduced into a Japanese Cuisine restaurant to improve it. Results show that t...

  1. Implant Surface Design Regulates Mesenchymal Stem Cell Differentiation and Maturation.

    Science.gov (United States)

    Boyan, B D; Cheng, A; Olivares-Navarrete, R; Schwartz, Z

    2016-03-01

    Changes in dental implant materials, structural design, and surface properties can all affect biological response. While bulk properties are important for mechanical stability of the implant, surface design ultimately contributes to osseointegration. This article reviews the surface parameters of dental implant materials that contribute to improved cell response and osseointegration. In particular, we focus on how surface design affects mesenchymal cell response and differentiation into the osteoblast lineage. Surface roughness has been largely studied at the microscale, but recent studies have highlighted the importance of hierarchical micron/submicron/nanosurface roughness, as well as surface roughness in combination with surface wettability. Integrins are transmembrane receptors that recognize changes in the surface and mediate downstream signaling pathways. Specifically, the noncanonical Wnt5a pathway has been implicated in osteoblastic differentiation of cells on titanium implant surfaces. However, much remains to be elucidated. Only recently have studies been conducted on the differences in biological response to implants based on sex, age, and clinical factors; these all point toward differences that advocate for patient-specific implant design. Finally, challenges in implant surface characterization must be addressed to optimize and compare data across studies. An understanding of both the science and the biology of the materials is crucial for developing novel dental implant materials and surface modifications for improved osseointegration. © International & American Associations for Dental Research 2016.

  2. Proton exchange fuel cell : the design, construction and evaluation

    Energy Technology Data Exchange (ETDEWEB)

    Heinzen, M.R.; Simoes, G.C.; Da Silva, L. [Univ. do Vale do Itajai, Sao Jose, SC (Brazil). Lab. de Pesquisa em Energia; Fiori, M.A.; Paula, M.M.S. [Univ. do Extremo Sul Catarinense, Santa Catarina (Brazil). Lab. de Sintese de Complexos Multifuncionais; Benavides, R. [Centro de Investigacion en Quimica Aplicada, Coahuila (Mexico)

    2010-07-15

    Polymer electrolyte membrane fuel cells (PEMFC) convert the chemical energy stored in the fuel directly into electrical energy without intermediate steps. The PEMFC operates at a relatively low operating temperature making it a good choice for mobile applications, but a high power density is needed in order to decrease the total weight of the vehicles. This paper presented a simple methodology to construct a PEMFC-type fuel cell, with particular reference to the gaseous diffuser, cell structure, the fixing plate, mounting bracket, gas distribution plates, and the membrane electrode assembly (MEA). The geometric design and meshing of the PEMFC were also described. The electrode was made using graphite with flow-field geometry. The PEMFC was tested for 100 hour of continuous work, during which time the current and voltage produced were monitored in order to evaluate the performance of the PEMFC. The materials used in the preparation of the fuel cell proved to be suitable. There was no loss of efficiency during the tests. The most relevant aspects affecting the PEMFC design were examined in an effort to optimize the performance of the cell. 13 refs., 6 figs.

  3. Optimal designs of bioretention cells in shallow groundwater

    Science.gov (United States)

    Zhang, K.; Chui, T. F. M.

    2017-12-01

    Bioretention cells, as one representative low impact development practices, have been proved to be effective in controlling surface runoff, removing pollutants and recharging groundwater. However, they are often not recommended in shallow groundwater areas due to potential groundwater pollution, reduction in runoff control performance and groundwater drainage through the underdrain. Most design guidelines only require a minimum distance between bioretention cell bottom and seasonal high groundwater table without guiding the design of bioretention cells to mitigate the problem of shallow groundwater. This study therefore proposed some design recommendations of bioretention cells for different rainfall runoff loads, native soil types and initial water table depths. A variably saturated flow model was employed to conduct event-based simulations on one single hypothetical bioretention cell in shallow groundwater, which was calibrated using experimental and simulation data of an on-site bioretention cell. A wide range of climatic and geophysical factors (i.e. initial groundwater depths, native soils, rainfall runoff loads) and bioretention designs (i.e. media soil types and underdrain sizes) were considered. Surface runoff reduction, time before groundwater mound formation, as well as maximum height of groundwater mound were evaluated. Less-permeable media types (i.e. sandy loam) are recommended in areas with many extreme rainfall events (i.e. 40 - 70 mm/h or larger) and of shallower groundwater, which can better protect groundwater from mounding and possibly contamination although may slightly compromise the runoff control performance. For areas having seasonal high groundwater table of 0 - 1 m below bioretention bottom, underdrain is recommended to maintain good infiltration capacity without draining groundwater. However, underdrain is not recommended for areas of groundwater table always near or above the bioretention bottom, only if an impermeable sheet is added

  4. Design optimization of a helical endothelial cell culture device.

    Science.gov (United States)

    Van Doormaal, Mark A; Ethier, C Ross

    2010-10-01

    The specific roles of mass transfer and fluid dynamic stresses on endothelial function, important in atherogenesis, are not known. Further, the effects of mass transfer and fluid dynamic stresses are difficult to separate because areas of "abnormal" mass transfer and "abnormal" wall shear stress tend to co-localize (where abnormal is defined as any deviation from the mass transfer rate or wall shear stress present in a long straight artery with the same flow rate and diameter). Our goal was to design a cell culture device which gives maximum separation between areas of abnormal shear stress and areas of abnormal mass transfer. We used design optimization principles to design a helical cell culture device. Using shear stress and mass transfer fields predicted by solving the governing equations, the area of the device which was exposed to low rates of mass transfer and normal levels of wall shear stress was determined. The design optimization method then maximized this area by varying the design variables, resulting in the optimum design. The optimum design had Reynolds number = 50, helical radius = 3.23 and helical pitch = 3.82. The area of the device which was exposed to low rates of mass transfer and regular levels of wall shear stress was about 4.5 times the inlet cross-sectional area of the device or about 5% of the device total internal surface area. An optimum design was successfully determined and the methodology used was shown to be robust. The area of the device which was exposed to low rates of mass transfer and regular levels of wall shear stress occurred in a defined region which should aid further experimental work.

  5. Design Methods and Factors Influencing their Uptake in Product Development Companies: A Review

    OpenAIRE

    Jagtap, Santosh; Warell, Anders; Hiort, Viktor; Motte, Damien; Larsson, Andreas

    2014-01-01

    Design methods are necessary to support product development companies in improving their product development processes, and thereby in producing high quality products. The uptake of design methods in product development companies is limited and lower than one might expect. There is a wide variety of literature related to factors that influence the uptake of design methods in companies. In this paper, we synthesised this literature, and in particular, developed a framework of factors than can ...

  6. An investigation into the Eco-design of Energy Using Products directive.

    Science.gov (United States)

    Meech, Christina Goodrick

    2006-01-01

    This study reviews the eco-design of energy using products directive and additional legislation on the way that waste electrical and electronic equipment should be disposed of, and how this may influence the future product design of appliances. During the investigation 'lifecycle analysis' and consumers' responses to this legislation, and the industry were also reviewed. Finally, for future product designers who work with electrical and electronic equipment some guidelines and rules are given to consider while designing for the environment and sustainability.

  7. 77 FR 72653 - Designation of Product Categories for Federal Procurement

    Science.gov (United States)

    2012-12-05

    ... evaluation process allows it to establish minimum biobased contents based on a broad set of factors to assist... the relevant analyses performed during the selection of these product categories. In addition, USDA... information for at least five different suppliers of products within each product category in today's proposed...

  8. Dexamethasone inhibits IL-9 production by human T cells

    Directory of Open Access Journals (Sweden)

    Cormont Francoise

    2005-04-01

    Full Text Available Abstract Background Interleukin 9 (IL-9 is produced by activated CD4+ T cells. Its effects include stimulation of mucus production, enhanced mast cell proliferation, enhanced eosinophil function, and IgE production. These effects are consistent with a role in allergic diseases. Glucocorticoids have potent anti-inflammatory effects, including suppression of cytokine synthesis, and are widely used in the treatment of allergic conditions. Methods We examined the effect of the glucocorticoid dexamethasone (Dex on IL-9 mRNA expression and protein secretion with real-time RT-PCR and ELISA. Peripheral blood mononuclear cells (PBMC were prepared from human volunteers and activated with OKT3. CD4+ T cells were purified from PBMC and activated with OKT3 plus PMA. Results IL-9 mRNA abundance and protein secretion were both markedly reduced following treatment of activated PBMC with Dex. mRNA levels were reduced to 0.7% of control values and protein secretion was reduced to 2.8% of controls. In CD4+ T cells, Dex reduced protein secretion to a similar extent. The IC50 value of Dex on mRNA expression was 4 nM. Conclusion These results indicate that IL-9 production is very markedly inhibited by Dex. The findings raise the possibility that the beneficial effects of glucocorticoids in the treatment of allergic diseases are in part mediated by inhibition of IL-9 production.

  9. A Knowledge Modeling Method for Computer Graphics Design & Production Based on Ontology

    Directory of Open Access Journals (Sweden)

    Chen Tong

    2017-01-01

    Full Text Available As one of the most critical stages of CG (Computer Graphics industry, CG design & production needs the support of professional knowledge and practice experience of multidisciplinary. With the outstanding performance in knowledge sharing, integration and reuse, knowledge modeling could increase greatly the efficiency, reduce the cost and avoid repeated error in CG design & production. However, knowledge modeling of CG design & production differs greatly from those of other fields. On the one hand, it is similar to physical product design, which involves great deal of tacit knowledge such as modeling skills, reasoning knowledge and so on. On the other hand, as film, CG design & production needs a lot of unstructured description information. The heterogeneity between physical product and film makes knowledge modeling more complicated. Thus a systematic knowledge modelling method based on Ontology is proposed to aid CG design & production in this paper. CG animation knowledge is capture and organized from viewpoint of three aspects: requirements and design and production. The knowledge are categorized into static and dynamic knowledge, and Ontology is adopted to construct a hierarchic model to organize the knowledge, so as to offer a uniform communication semantic foundations for designers from different fields. Based on animation script, the CG design task model is proposed to drive the organization and management of different knowledge involved in CG design & production. Finally, we apply this method in the knowledge modeling of naked-eye animation design and production to illustrate effectiveness of this method.

  10. Ethanol Production from Biomass: Large Scale Facility Design Project

    Energy Technology Data Exchange (ETDEWEB)

    Berson, R. Eric [Univ. of Louisville, KY (United States)

    2009-10-29

    High solids processing of biomass slurries provides the following benefits: maximized product concentration in the fermentable sugar stream, reduced water usage, and reduced reactor size. However, high solids processing poses mixing and heat transfer problems above about 15% for pretreated corn stover solids due to their high viscosities. Also, highly viscous slurries require high power consumption in conventional stirred tanks since they must be run at high rotational speeds to maintain proper mixing. An 8 liter scraped surface bio-reactor (SSBR) is employed here that is designed to efficiently handle high solids loadings for enzymatic saccharification of pretreated corn stover (PCS) while maintaining power requirements on the order of low viscous liquids in conventional stirred tanks. Saccharification of biomass exhibit slow reaction rates and incomplete conversion, which may be attributed to enzyme deactivation and loss of activity due to a variety of mechanisms. Enzyme deactivation is classified into two categories here: one, deactivation due to enzyme-substrate interactions and two, deactivation due to all other factors that are grouped together and termed “non-specific” deactivation. A study was conducted to investigate the relative extents of “non-specific” deactivation and deactivation due to “enzyme-substrate interactions” and a model was developed that describes the kinetics of cellulose hydrolysis by considering the observed deactivation effects. Enzyme substrate interactions had a much more significant effect on overall deactivation with a deactivation rate constant about 20X higher than the non-specific deactivation rate constant (0.35 h-1 vs 0.018 h-1). The model is well validated by the experimental data and predicts complete conversion of cellulose within 30 hours in the absence of enzyme substrate interactions.

  11. Apparatus of hot cell for iodine-123 production

    International Nuclear Information System (INIS)

    Almeida, G.L. de; Rautenberg, F.A.; Souza, A.S.F. de.

    1986-01-01

    The hot cell installation at IEN cyclotron (Brazilian-CNEN) for sup(123)I production is presented. Several devices, such as, tube furnace coupling system, tube furnace driving system, sup(123)I target transfer system, product extraction system, furnace control system, and effluent systems, were constructed and modified for implanting process engineering. The requirements of safety engineering for operation process were based on ALARA concept. (M.C.K.)

  12. Robo signaling regulates the production of cranial neural crest cells.

    Science.gov (United States)

    Li, Yan; Zhang, Xiao-Tan; Wang, Xiao-Yu; Wang, Guang; Chuai, Manli; Münsterberg, Andrea; Yang, Xuesong

    2017-12-01

    Slit/Robo signaling plays an important role in the guidance of developing neurons in developing embryos. However, it remains obscure whether and how Slit/Robo signaling is involved in the production of cranial neural crest cells. In this study, we examined Robo1 deficient mice to reveal developmental defects of mouse cranial frontal and parietal bones, which are derivatives of cranial neural crest cells. Therefore, we determined the production of HNK1 + cranial neural crest cells in early chick embryo development after knock-down (KD) of Robo1 expression. Detection of markers for pre-migratory and migratory neural crest cells, PAX7 and AP-2α, showed that production of both was affected by Robo1 KD. In addition, we found that the transcription factor slug is responsible for the aberrant delamination/EMT of cranial neural crest cells induced by Robo1 KD, which also led to elevated expression of E- and N-Cadherin. N-Cadherin expression was enhanced when blocking FGF signaling with dominant-negative FGFR1 in half of the neural tube. Taken together, we show that Slit/Robo signaling influences the delamination/EMT of cranial neural crest cells, which is required for cranial bone development. Copyright © 2017. Published by Elsevier Inc.

  13. Speeding the transition: Designing a fuel-cell hypercar

    Energy Technology Data Exchange (ETDEWEB)

    Williams, B.D.; Moore, T.C.; Lovins, A.B. [Rocky Mountain Inst., Snowmass, CO (United States). Hypercar Center

    1997-12-31

    A rapid transformation now underway in automotive technology could accelerate the transition to transportation powered by fuel cells. Ultralight, advanced-composite, low-drag, hybrid-electric hypercars--using combustion engines--could be three- to fourfold more efficient and one or two orders of magnitude cleaner than today`s cars, yet equally safe, sporty, desirable, and (probably) affordable. Further, important manufacturing advantages--including low tooling and equipment costs, greater mechanical simplicity, autobody parts consolidation, shorter product cycles, and reduced assembly effort and space--permit a free-market commercialization strategy. This paper discusses a conceptual hypercar powered by a proton-exchange-membrane fuel cell (PEMFC). It outlines the implications of platform physics and component selection for the vehicle`s mass budget and performance. The high fuel-to-traction conversion efficiency of the hypercar platform could help automakers overcome the Achilles` heel of hydrogen-powered vehicles: onboard storage. Moreover, because hypercars would require significantly less tractive power, and even less fuel-cell power, they could adopt fuel cells earlier, before fuel cells` specific cost, mass, and volume have fully matured. In the meantime, commercialization in buildings can help prepare fuel cells for hypercars. The promising performance of hydrogen-fueled PEMFC hypercars suggests important opportunities in infrastructure development for direct-hydrogen vehicles.

  14. Statistical optimisation of cell growth and carotenoid production by Rhodotorula mucilaginosa

    Directory of Open Access Journals (Sweden)

    Iriani R. Maldonade

    2012-03-01

    Full Text Available Sequential statistical methods were used to maximise carotenoid production by a strain of Rhodotorula mucilaginosa, isolated from the Brazilian ecosystem. Initially, a factorial 2(5-1 experimental design was used, and the variables were pH and the levels of glucose, yeast extract, MgSO4.7H2O and KH2PO4. The nitrogen source (yeast extract was the most important variable in enhancing carotenoid production; MgSO4.7H2O and KH2PO4 had a negative influence. The initial pH had no significant effect on carotenoid and cell productions. We further investigated the effects of glucose and yeast extract effects, using a second-order central composite design (CCD to optimise carotenoid production, which was adequately approximated with a full quadratic equation obtained from a two-factor-2-level design. The analysis of quadratic surfaces showed that after 5 days of cultivation at 25ºC, the maximum carotenoid concentration (745 µg l-1 was obtained with 15 g l-1 of yeast extract and 20 g l-1 of glucose. The maximum carotenoid production (152 µg g-1 was obtained with 5 g l-1 yeast extract and 10 g l-1 glucose. Carotenoid formation was more sensitive to changes in yeast extract concentration than to changes in glucose concentration. Maximum cell production was achieved with 15-17 g l-1 of yeast extract and 15-20 g l-1 of glucose.

  15. Photobiological hydrogen production with switchable photosystem-II designer algae

    Science.gov (United States)

    Lee, James Weifu

    2014-02-18

    A process for enhanced photobiological H.sub.2 production using transgenic alga. The process includes inducing exogenous genes in a transgenic alga by manipulating selected environmental factors. In one embodiment inducing production of an exogenous gene uncouples H.sub.2 production from existing mechanisms that would downregulate H.sub.2 production in the absence of the exogenous gene. In other embodiments inducing an exogenous gene triggers a cascade of metabolic changes that increase H.sub.2 production. In some embodiments the transgenic alga are rendered non-regenerative by inducing exogenous transgenes for proton channel polypeptides that are targeted to specific algal membranes.

  16. MERIP - Design of Production Systems with focus on Human Ressources

    DEFF Research Database (Denmark)

    Jacobsen, Peter; Knudsen, Mads Kristian Lund

    1998-01-01

    and production. This development has been a continuos process, and it has been supported by several research- and development projects in co-operation with Danish Industry, CO industry and The Technical University of Denmark. Among the projects should be mentioned: "Development of Production systems - UPS......", "Employee-activated Production Development - MAPU", "Integrated Production Systems - IPS" and "Strategic Development of Staff - SUM". MERIP (Human resources in production) is a continuation of this type of projects, aiming at increasing the competitive power of the companies. However MERIP scientists want...

  17. HYDROGEN PRODUCTION FOR FUEL CELLS VIA REFORMING COAL-DERIVED METHANOL

    Energy Technology Data Exchange (ETDEWEB)

    Paul A. Erickson

    2006-01-01

    Hydrogen can be produced from many feedstocks including coal. The objectives of this project are to establish and prove a hydrogen production pathway from coal-derived methanol for fuel cell applications. This progress report is the ninth report submitted to the DOE reporting on the status and progress made during the course of the project. This report covers the time period of October 1, 2005-December 31, 2005. This quarter saw progress in four areas. These areas are: (1) reformate purification, (2) heat transfer enhancement, (3) autothermal reforming coal-derived methanol degradation test; and (4) model development for fuel cell system integration. The project is on schedule and is now shifting towards the design of an integrated PEM fuel cell system capable of using the coal-derived product. This system includes a membrane clean up unit and a commercially available PEM fuel cell.

  18. Design optimization of jacket structures for mass production

    DEFF Research Database (Denmark)

    Sandal, Kasper

    conceptual designs. Stress concentrations in welds are modelled using design dependent stress concentration factors. Simplified models are also implemented for both piled foundations and suction caissons. Wind and wave loads are applied according to a realistic offshore environment. An optimal design problem...

  19. Plasmonic Solar Cells: From Rational Design to Mechanism Overview.

    Science.gov (United States)

    Jang, Yoon Hee; Jang, Yu Jin; Kim, Seokhyoung; Quan, Li Na; Chung, Kyungwha; Kim, Dong Ha

    2016-12-28

    Plasmonic effects have been proposed as a solution to overcome the limited light absorption in thin-film photovoltaic devices, and various types of plasmonic solar cells have been developed. This review provides a comprehensive overview of the state-of-the-art progress on the design and fabrication of plasmonic solar cells and their enhancement mechanism. The working principle is first addressed in terms of the combined effects of plasmon decay, scattering, near-field enhancement, and plasmonic energy transfer, including direct hot electron transfer and resonant energy transfer. Then, we summarize recent developments for various types of plasmonic solar cells based on silicon, dye-sensitized, organic photovoltaic, and other types of solar cells, including quantum dot and perovskite variants. We also address several issues regarding the limitations of plasmonic nanostructures, including their electrical, chemical, and physical stability, charge recombination, narrowband absorption, and high cost. Next, we propose a few potentially useful approaches that can improve the performance of plasmonic cells, such as the inclusion of graphene plasmonics, plasmon-upconversion coupling, and coupling between fluorescence resonance energy transfer and plasmon resonance energy transfer. This review is concluded with remarks on future prospects for plasmonic solar cell use.

  20. From Design to Production Control Through the Integration of Engineering Data Management and Workflow Management Systems

    CERN Document Server

    Le Goff, J M; Bityukov, S; Estrella, F; Kovács, Z; Le Flour, T; Lieunard, S; McClatchey, R; Murray, S; Organtini, G; Vialle, J P; Bazan, A; Chevenier, G

    1997-01-01

    At a time when many companies are under pressure to reduce "times-to-market" the management of product information from the early stages of design through assembly to manufacture and production has become increasingly important. Similarly in the construction of high energy physics devices the collection of ( often evolving) engineering data is central to the subsequent physics analysis. Traditionally in industry design engineers have employed Engineering Data Management Systems ( also called Product Data Management Systems) to coordinate and control access to documented versions of product designs. However, these systems provide control only at the collaborative design level and are seldom used beyond design. Workflow management systems, on the other hand, are employed in industry to coordinate and support the more complex and repeatable work processes of the production environment. Commer cial workflow products cannot support the highly dynamic activities found both in the design stages of product developmen...