WorldWideScience

Sample records for cell model library

  1. Cell Libraries

    Science.gov (United States)

    1994-01-01

    A NASA contract led to the development of faster and more energy efficient semiconductor materials for digital integrated circuits. Gallium arsenide (GaAs) conducts electrons 4-6 times faster than silicon and uses less power at frequencies above 100-150 megahertz. However, the material is expensive, brittle, fragile and has lacked computer automated engineering tools to solve this problem. Systems & Processes Engineering Corporation (SPEC) developed a series of GaAs cell libraries for cell layout, design rule checking, logic synthesis, placement and routing, simulation and chip assembly. The system is marketed by Compare Design Automation.

  2. An open-source library for the numerical modeling of mass-transfer in solid oxide fuel cells

    Science.gov (United States)

    Novaresio, Valerio; García-Camprubí, María; Izquierdo, Salvador; Asinari, Pietro; Fueyo, Norberto

    2012-01-01

    The generation of direct current electricity using solid oxide fuel cells (SOFCs) involves several interplaying transport phenomena. Their simulation is crucial for the design and optimization of reliable and competitive equipment, and for the eventual market deployment of this technology. An open-source library for the computational modeling of mass-transport phenomena in SOFCs is presented in this article. It includes several multicomponent mass-transport models ( i.e. Fickian, Stefan-Maxwell and Dusty Gas Model), which can be applied both within porous media and in porosity-free domains, and several diffusivity models for gases. The library has been developed for its use with OpenFOAM ®, a widespread open-source code for fluid and continuum mechanics. The library can be used to model any fluid flow configuration involving multicomponent transport phenomena and it is validated in this paper against the analytical solution of one-dimensional test cases. In addition, it is applied for the simulation of a real SOFC and further validated using experimental data. Program summaryProgram title: multiSpeciesTransportModels Catalogue identifier: AEKB_v1_0 Program summary URL:http://cpc.cs.qub.ac.uk/summaries/AEKB_v1_0.html Program obtainable from: CPC Program Library, Queen's University, Belfast, N. Ireland Licensing provisions: GNU General Public License No. of lines in distributed program, including test data, etc.: 18 140 No. of bytes in distributed program, including test data, etc.: 64 285 Distribution format: tar.gz Programming language:: C++ Computer: Any x86 (the instructions reported in the paper consider only the 64 bit case for the sake of simplicity) Operating system: Generic Linux (the instructions reported in the paper consider only the open-source Ubuntu distribution for the sake of simplicity) Classification: 12 External routines: OpenFOAM® (version 1.6-ext) ( http://www.extend-project.de) Nature of problem: This software provides a library of models for

  3. CellLab-CTS 2015: a Python library for continuous-time stochastic cellular automaton modeling using Landlab

    Science.gov (United States)

    Tucker, G. E.; Hobley, D. E. J.; Hutton, E.; Gasparini, N. M.; Istanbulluoglu, E.; Adams, J. M.; Nudurupati, S. S.

    2015-11-01

    CellLab-CTS 2015 is a Python-language software library for creating two-dimensional, continuous-time stochastic (CTS) cellular automaton models. The model domain consists of a set of grid nodes, with each node assigned an integer state-code that represents its condition or composition. Adjacent pairs of nodes may undergo transitions to different states, according to a user-defined average transition rate. A model is created by writing a Python code that defines the possible states, the transitions, and the rates of those transitions. The code instantiates, initializes, and runs one of four object classes that represent different types of CTS model. CellLab-CTS provides the option of using either square or hexagonal grid cells. The software provides the ability to treat particular grid-node states as moving particles, and to track their position over time. Grid nodes may also be assigned user-defined properties, which the user can update after each transition through the use of a callback function. As a component of the Landlab modeling framework, CellLab-CTS models take advantage of a suite of Landlab's tools and capabilities, such as support for standardized input and output.

  4. Library 2.0 A New Service Model For Libraries

    Directory of Open Access Journals (Sweden)

    Agnes Erich

    2007-01-01

    Full Text Available The utilization of the new information and communication technologies implies a series of changes concerning the libraries. Currently it is spoken more and more about a new type of service offered by them, Library 2.0, which brings in foreground the user who becomes a modeler of the library services and resources. The information structures customer is not satisfied anymore by the unidirectional access to the resources, he wanted to be involved in the evaluation of them, making comments and sharing his ideas with another user. In this sense the library managers are looking for more efficient means to deliver services so that to obtain a proper feedback which enable them to take the best measures to make profitable them. Library 2.0 can be a solution for all these problems.

  5. A Circulation Model for Busy Public Libraries.

    Science.gov (United States)

    Bagust, A.

    1983-01-01

    Develops stochastic model of library borrowing using Negative Binomial distribution applied to circulation data obtained from Huddersfield Public Library. Evidence concerning process of popularity decay is presented and method is given by which relegation tests can be constructed to maintain optimum turnover. Eight references and statistical…

  6. Adiabatic quantum-flux-parametron cell library adopting minimalist design

    Energy Technology Data Exchange (ETDEWEB)

    Takeuchi, Naoki, E-mail: takeuchi-naoki-kx@ynu.jp [Institute of Advanced Sciences, Yokohama National University, 79-5 Tokiwadai, Hodogaya, Yokohama 240-8501 (Japan); Yamanashi, Yuki; Yoshikawa, Nobuyuki [Institute of Advanced Sciences, Yokohama National University, 79-5 Tokiwadai, Hodogaya, Yokohama 240-8501 (Japan); Department of Electrical and Computer Engineering, Yokohama National University, 79-5 Tokiwadai, Hodogaya, Yokohama 240-8501 (Japan)

    2015-05-07

    We herein build an adiabatic quantum-flux-parametron (AQFP) cell library adopting minimalist design and a symmetric layout. In the proposed minimalist design, every logic cell is designed by arraying four types of building block cells: buffer, NOT, constant, and branch cells. Therefore, minimalist design enables us to effectively build and customize an AQFP cell library. The symmetric layout reduces unwanted parasitic magnetic coupling and ensures a large mutual inductance in an output transformer, which enables very long wiring between logic cells. We design and fabricate several logic circuits using the minimal AQFP cell library so as to test logic cells in the library. Moreover, we experimentally investigate the maximum wiring length between logic cells. Finally, we present an experimental demonstration of an 8-bit carry look-ahead adder designed using the minimal AQFP cell library and demonstrate that the proposed cell library is sufficiently robust to realize large-scale digital circuits.

  7. Adiabatic quantum-flux-parametron cell library adopting minimalist design

    International Nuclear Information System (INIS)

    We herein build an adiabatic quantum-flux-parametron (AQFP) cell library adopting minimalist design and a symmetric layout. In the proposed minimalist design, every logic cell is designed by arraying four types of building block cells: buffer, NOT, constant, and branch cells. Therefore, minimalist design enables us to effectively build and customize an AQFP cell library. The symmetric layout reduces unwanted parasitic magnetic coupling and ensures a large mutual inductance in an output transformer, which enables very long wiring between logic cells. We design and fabricate several logic circuits using the minimal AQFP cell library so as to test logic cells in the library. Moreover, we experimentally investigate the maximum wiring length between logic cells. Finally, we present an experimental demonstration of an 8-bit carry look-ahead adder designed using the minimal AQFP cell library and demonstrate that the proposed cell library is sufficiently robust to realize large-scale digital circuits

  8. No Phone Zone: Controlling Cell Phone Use in Academic Libraries

    Science.gov (United States)

    Heaton, Shelley; Master, Nancy

    2006-01-01

    This article reports the results of a survey of cell phone policies in university libraries conducted over a three-month period in 2005. The authors sought information about the libraries' problems regarding cell phone use and attendant noise by students and other library users. The authors began with an 18-question survey of one hundred…

  9. An effective timing characterization method for an accuracy-proved VLSI standard cell library

    International Nuclear Information System (INIS)

    This paper presents a method of tailoring the characterization and modeling timing of a VLSI standard cell library. The paper also presents a method to validate the reasonability of the value through accuracy analysis. In the process of designing a standard cell library, this method is applied to characterize the cell library. In addition, the error calculations of some simple circuit path delays are compared between using the characterization file and an Hspice simulation. The comparison results demonstrate the accuracy of the generated timing library file. (semiconductor integrated circuits)

  10. Arrangement of library materials models in Slovene public libraries

    Directory of Open Access Journals (Sweden)

    Marija Kobal

    2009-01-01

    Full Text Available The classification and the alignment of library material represent an important part of the library science from its early beginnings onwards. The introduction of UDC into Slovene public libraries coincided with the alteration of the open access library collection.In Slovenia the public libraries in average take into account the references and recommendations for the arrangement of the free access library collection, considering the age groups of the users, adults and juniors. The next division is connected with the functional type of the material, which is divided into fiction, and scientific material,and then there is the UDC label for the arrangement of the material on the shelves or word systematic in one of the public libraries. In the junior department there is a need for a further division into four age/reading groups of books instead of three.Within the formed groups there is the further arrangement of books in an alphabetical order by means of author or subject headings. The public library is a unit, which services all the residents in its local area. The different needs and interests of users should take into account in the formation of a library material arrangement.

  11. Indian Consortia Models: FORSA Libraries' Experiences

    Science.gov (United States)

    Patil, Y. M.; Birdie, C.; Bawdekar, N.; Barve, S.; Anilkumar, N.

    2007-10-01

    With increases in prices of journals, shrinking library budgets and cuts in subscriptions to journals over the years, there has been a big challenge facing Indian library professionals to cope with the proliferation of electronic information resources. There have been sporadic efforts by different groups of libraries in forming consortia at different levels. The types of consortia identified are generally based on various models evolved in India in a variety of forms depending upon the participants' affiliations and funding sources. Indian astronomy library professionals have formed a group called Forum for Resource Sharing in Astronomy and Astrophysics (FORSA), which falls under `Open Consortia', wherein participants are affiliated to different government departments. This is a model where professionals willingly come forward and actively support consortia formation; thereby everyone benefits. As such, FORSA has realized four consortia, viz. Nature Online Consortium; Indian Astrophysics Consortium for physics/astronomy journals of Springer/Kluwer; Consortium for Scientific American Online Archive (EBSCO); and Open Consortium for Lecture Notes in Physics (Springer), which are discussed briefly.

  12. Design for manufacturability of a VDSM standard cell library

    Institute of Scientific and Technical Information of China (English)

    周宠; 陈岚; 曾健平; 尹明会; 赵劫

    2012-01-01

    This paper presents a method of designing a 65 nm DFM standard cell library.By reducing the amount of the library largely,the process of optical proximity correction (OPC) becomes more efficient and the need for large storage is reduced.This library is more manufacture-friendly as each cell has been optimized according to the DFM rule and optical simulation.The area penalty is minor compared with traditional library,and the timing,as well as power has a good performance.Furthermore,this library has passed the test from the Technology Design Department of Foundry.The result shows this DFM standard cell library has advantages that improve the yield.

  13. Outline and Preliminary Evaluation of the Classical Digital Library Model.

    Science.gov (United States)

    MacCall, Steven L.; Cleveland, Ana D.; Gibson, Ian E.

    1999-01-01

    Outlines the classical digital library model, which is derived from traditional practices of library and information science professionals, as an alternative to the database retrieval model. Reports preliminary results from an evaluation study of library and information professionals and endusers involved with primary care medicine. (AEF)

  14. On foundations of typed data models for digital libraries

    OpenAIRE

    Candela, Leonardo; Castelli, Donatella; Manghi, Paolo; Mikulicic, Marko; Pagano, Pasquale

    2009-01-01

    This work discusses the foundational aspects of a compound object data model for Digital Libraries Management Systems whose novelty is to reintroduce the notion of types as a mean of supporting safe, optimized and efficient implementations of Digital Library Systems.

  15. Analysis of boundary point (break point) in Linear Delay Model for nanoscale VLSI standard cell library characterization at PVT corners

    CERN Document Server

    Agarwal, Gaurav Kumar

    2014-01-01

    In VLSI chip design flow, Static Timing Analysis (STA) is used for fast and accurate analysis of data-path delay. This process is fast because delay is picked from Look Up Tables (LUT) rather than conventional SPICE simulations. But accuracy of this method depends upon the underlying delay model with which LUT was characterized. Non Linear Delay Model (NLDM) based LUTs are quite common in industries. These LUT requires huge amount to time during characterization because of huge number of SPICE simulations done at arbitrary points. To improve this people proposed various other delay models like alpha-power and piecewise linear delay models. Bulusu et al proposed Linear Delay Model(LDM) which reduces LUT generation time to 50 percent. LDM divides delay curve w.r.t input rise time(trin) into two different region one is linear and other is non-linear. This boundary point between linear and non- linear region was called break point (trb). Linear region will be done if we simulate at only two points. This advantage...

  16. Where's the Library in Service Learning?: Models for Engaged Library Instruction.

    Science.gov (United States)

    Riddle, John S.

    2003-01-01

    Discusses service learning and presents models of instruction that academic libraries might offer to service learning pedagogy. Highlights include the role of information literacy; reflection; affective and cognitive learning outcomes of service learning; impact of service learning on libraries; learning process model; course objectives model;…

  17. Data Collection and Cost Modeling for Library Circulation Systems.

    Science.gov (United States)

    Bourne, Charles P.

    The objectives of the study leading to this report were to review, analyze and summarize published library cost data; and to develop a cost model and a methodology for reporting data in a more consistent and useful way. The cost model and reporting procedure were developed and tested on the circulation system of three libraries: a large university…

  18. Blended Library and Multimedia Model in Geography Teaching

    OpenAIRE

    Asadi, Saeid; Hamid R. Jamali

    2011-01-01

    Libraries have been established and always utilized in old fashion and modern school and academic environments in order to support educational programs. However, new instructional technologies have changed traditional library-based teaching models. Multimedia approach has shown successful in teaching different courses to different learners. However, there is little known how a combination of library and multimedia approach can be implemented and measured in teaching. This paper focuses on an ...

  19. A Components Library System Model and the Support Tool

    Institute of Scientific and Technical Information of China (English)

    MIAO Huai-kou; LIU Hui; LIU Jing; LI Xiao-bo

    2004-01-01

    Component-based development needs a well-designed components library and a set of support tools.This paper presents the design and implementation of a components library system model and its support tool UMLCASE.A set of practical CASE tools is constructed.UMLCASE can use UML to design Use Case Diagram, Class Diagram etc.And it integrates with components library system.

  20. Synthetic Peptide libraries for T-cell epitope identification.

    Science.gov (United States)

    Hiemstra, H S; Drijfhout, J W; Koning, F

    2000-01-01

    This chapter describes a methodology for elucidating immunogenic epitopes stimulatory for CD4(+) T-cell clones (Fig. 1). The methodology makes use of synthetic peptide libraries and must be regarded as an alternative to other approaches, such as peptide elution or the application of genetic libraries. The methodology only requires knowledge about the restriction element of the T-cell clone. The restriction element determines which major histocompatibility complex (MHC)-binding anchor motif must be built into the library peptides. A synthetic peptide library is prepared comprising approx 8 million peptides. The synthesis proceeds via a mix-and-split protocol using a solidphase approach on a hybrid resin (1,2). On a hybrid resin, most of the peptide material (84%) is attached via an acid-labile linker whereas the remaining part of the peptide material is acid-stable attached (3). During synthesis, resinbound peptides comprising 14 amino acid residues are produced, with each resin bead containing one unique peptide (4,5). The beads are split into 384 pools, with each pool containing 20,000 beads. From each pool, about 28% of the peptide material is cleaved from every bead. Subsequently, in the first screening round, the 384 pools, each containing 20,000 solubilized peptides, are tested in a proliferation assay with the T-cell clone. Fig. 1. Flow diagram of the complete procedure for the identification of T-cell epitopes using synthetic peptide libraries (1).

  1. Modeling and Implementing ISO 13584-based Part Library

    Institute of Scientific and Technical Information of China (English)

    杨东; 肖丽雯; 何援军; 张申生

    2004-01-01

    ISO 13584 (I.e. PLIB) is an international standard for the representation and exchange of CAD part libraries. It aims to provide an application-independent mechanism to enable the share of part library information between applications. In this paper, the approach of modeling part library conforming to ISO 13584 is presented. Also, a prototype of part library management system, I.e. BYL-PLIB, whose implementation is in agreement with ISO 13854 is developed to demonstrate the usefulness of proposed approach.

  2. Designing Public Library Websites for Teens: A Conceptual Model

    Science.gov (United States)

    Naughton, Robin Amanda

    2012-01-01

    The main goal of this research study was to develop a conceptual model for the design of public library websites for teens (TLWs) that would enable designers and librarians to create library websites that better suit teens' information needs and practices. It bridges a gap in the research literature between user interface design in…

  3. Public Libraries and Internet Public Access Models: Describing Possible Approaches.

    Science.gov (United States)

    Tomasello, Tami K.; McClure, Charles R.

    2002-01-01

    Discusses ways of providing Internet access to the general public and analyzes eight models currently in use: public schools, public libraries, cybermobiles, public housing, community technology centers, community networks, kiosks, and cyber cafes. Concludes that public libraries may wish to develop collaborative strategies with other…

  4. Using the Gamma-Poisson Model to Predict Library Circulations.

    Science.gov (United States)

    Burrell, Quentin L.

    1990-01-01

    Argues that the gamma mixture of Poisson processes, for all its perceived defects, can be used to make predictions regarding future library book circulations of a quality adequate for general management requirements. The use of the model is extensively illustrated with data from two academic libraries. (Nine references) (CLB)

  5. Functional Model of Higher Educational Institution Library Building up

    OpenAIRE

    Murat K. Baimul'din; Asemgul' S. Smagulova; Gulnur B. Abildaeva; Zagira B. Saimanova

    2013-01-01

    The article presents the technology of integrated data processing, related to academic and library-bibliographic activities for specialties work programs development and book sufficiency estimation in integrated research and information system of higher educational institution. The model of higher educational institution library building up, based on monitoring of educational process book sufficiency and literature demand is introduced

  6. Cloud Computing Platform for an Online Model Library System

    Directory of Open Access Journals (Sweden)

    Mingang Chen

    2013-01-01

    Full Text Available The rapid developing of digital content industry calls for online model libraries. For the efficiency, user experience, and reliability merits of the model library, this paper designs a Web 3D model library system based on a cloud computing platform. Taking into account complex models, which cause difficulties in real-time 3D interaction, we adopt the model simplification and size adaptive adjustment methods to make the system with more efficient interaction. Meanwhile, a cloud-based architecture is developed to ensure the reliability and scalability of the system. The 3D model library system is intended to be accessible by online users with good interactive experiences. The feasibility of the solution has been tested by experiments.

  7. Close the Textbook & Open "The Cell: An Image Library"

    Science.gov (United States)

    Saunders, Cheston; Taylor, Amy

    2014-01-01

    Many students leave the biology classroom with misconceptions centered on cellular structure. This article presents an activity in which students utilize images from an online database called "The Cell: An Image Library" (http://www.cellimagelibrary. org/) to gain a greater understanding of the diversity of cellular structure and the…

  8. Designing Multi-target Compound Libraries with Gaussian Process Models.

    Science.gov (United States)

    Bieler, Michael; Reutlinger, Michael; Rodrigues, Tiago; Schneider, Petra; Kriegl, Jan M; Schneider, Gisbert

    2016-05-01

    We present the application of machine learning models to selecting G protein-coupled receptor (GPCR)-focused compound libraries. The library design process was realized by ant colony optimization. A proprietary Boehringer-Ingelheim reference set consisting of 3519 compounds tested in dose-response assays at 11 GPCR targets served as training data for machine learning and activity prediction. We compared the usability of the proprietary data with a public data set from ChEMBL. Gaussian process models were trained to prioritize compounds from a virtual combinatorial library. We obtained meaningful models for three of the targets (5-HT2c , MCH, A1), which were experimentally confirmed for 12 of 15 selected and synthesized or purchased compounds. Overall, the models trained on the public data predicted the observed assay results more accurately. The results of this study motivate the use of Gaussian process regression on public data for virtual screening and target-focused compound library design.

  9. Making library linked data using the Europeana Data Model

    OpenAIRE

    Knepper, Marko; Charles, Valentine

    2014-01-01

    Europeana provides a common access point to digital cultural heritage objects across different cultural domains among which the libraries. The recent development of the Europeana Data Model (EDM) provide new ways for libraries to experiment with Linked Data. Indeed the model is designed as a framework reusing various wellknown standards developed in the Semantic Web Community, such as the Resource Description Framework (RDF), the OAI Object Reuse and Exchange (ORE), and Dublin Core namespaces...

  10. US Academic Libraries: Today's Learning Commons Model

    Science.gov (United States)

    McMullen, Susan

    2008-01-01

    In 2007, the author examined existing academic libraries in the United States to determine best practices for the design, implementation and service of learning commons facilities. A primary objective of this study was to discover how to create a higher education learning environment that sustains scholarship, encourages collaboration and empowers…

  11. A comprehensive library of familial human amyotrophic lateral sclerosis induced pluripotent stem cells.

    Directory of Open Access Journals (Sweden)

    Ying Li

    Full Text Available Amyotrophic lateral sclerosis is a progressive disease characterized by the loss of upper and lower motor neurons, leading to paralysis of voluntary muscles. About 10% of all ALS cases are familial (fALS, among which 15-20% are linked to Cu/Zn superoxide dismutase (SOD1 mutations, usually inherited in an autosomal dominant manner. To date only one FDA approved drug is available which increases survival moderately. Our understanding of ALS disease mechanisms is largely derived from rodent model studies, however due to the differences between rodents and humans, it is necessary to have humanized models for studies of disease pathogenesis as well as drug development. Therefore, we generated a comprehensive library of a total 22 of fALS patient-specific induced pluripotent stem cell (iPSC lines. These cells were thoroughly characterized before being deposited into the library. The library of cells includes a variety of C9orf72 mutations, sod1 mutations, FUS, ANG and FIG4 mutations. Certain mutations are represented with more than one line, which allows for studies of variable genetic backgrounds. In addition, these iPSCs can be successfully differentiated to astroglia, a cell type known to play a critical role in ALS disease progression. This library represents a comprehensive resource that can be used for ALS disease modeling and the development of novel therapeutics.

  12. Research on new information service model of the contemporary library

    International Nuclear Information System (INIS)

    According to the development of the internet and multimedia technology, the information service models in the contemporary library become both of the traditional and digital information service. The libraries in each country do their best to make the voluminous information and the complex technology be High-integrated in the background management, and also make the front interface be more and more convenient to the users. The essential characteristics of the information service of the contemporary library are all-in-one and humanness. In this article, we will describe several new hot information service models of the contemporary library in detail, such as individualized service, reference service, reference service and strategic information service. (authors)

  13. Using 4P Marketing Model in Academic Libraries: An Experience

    OpenAIRE

    Mehdi Alipour Hafezi; Hassan Ashrafi Rizi; Zahra Kazempour; Mahri Shahbazi

    2013-01-01

    The present study mainly aims to demonstrate the weaknesses and strengths of using marketing principles in academic libraries and finally presenting some important suggestions in order to improve their marketing. This study proceeds through expressing the current marketing situation of studied academic libraries from the view point of 4P model. In this regard, the current situation is analyzed using a researcher-made questionnaire, and then the problems and shortcomings are highlighted in the...

  14. Multiscale Computing with the Multiscale Modeling Library and Runtime Environment

    NARCIS (Netherlands)

    J. Borgdorff; M. Mamonski; B. Bosak; D. Groen; M. Ben Belgacem; K. Kurowski; A.G. Hoekstra

    2013-01-01

    We introduce a software tool to simulate multiscale models: the Multiscale Coupling Library and Environment 2 (MUSCLE 2). MUSCLE 2 is a component-based modeling tool inspired by the multiscale modeling and simulation framework, with an easy-to-use API which supports Java, C++, C, and Fortran. We pre

  15. Distraction and inattention in the driver model library

    NARCIS (Netherlands)

    Hogema, J.H.; Martens, M.H.; Ubink, E.M.

    2011-01-01

    TNO is developing a Driver Model Library (DML) to provide computational models of driver behaviour and decision making for use as a cross-platform plug-in for traffic simulations. The DML is based on a multi-agent cognitive framework which models the individual driving tasks (such as navigation, ove

  16. Modeling a National Collaborative Digital Library for Malaysian Secondary Schools

    Directory of Open Access Journals (Sweden)

    N.N. Edzan

    2004-09-01

    Full Text Available This paper describes the conceptualisation of a model for a collaborative digital library specially tailored for Malaysian secondary schools, which will support classroom teaching and learning. The move towards collaboratively building the contents of a digital library is a fairly recent trend and it simulates an environment where partners are empowered to participate in building and up keeping the knowledge contents of the system. The conceptualisation of a Malaysian digital library is in line with the governments efforts in establishing SMART schools. However, various issues such as identifying local resources, ascertaining the needs of it users, and establishing a framework to meet these needs, must be addressed before the digital library can be fully implemented. It may be approached through the establishment of test beds in a particular learning institution, before nationwide implementation.

  17. A Low Cost Rad-Tolerant Standard Cell Library

    Science.gov (United States)

    Gambles, Jody W.; Maki, Gary K.

    1997-01-01

    This paper describes circuit design techniques developed at the NASA Institute of Advanced Microelectronics that have been shown to protect CMOS circuits from the deleterious effects of the natural space radiation environment. The IAuE is leading a program to incorporate these radiation-tolerance providing design techniques into a commercial standard cell library that will be used in conjunction with available Electronic Design Automation tools to produce space flight qualified microelectronics fabricated at modern commercial CMOS foundries.

  18. Using 4P Marketing Model in Academic Libraries: An Experience

    Directory of Open Access Journals (Sweden)

    Mehdi Alipour Hafezi

    2013-06-01

    Full Text Available The present study mainly aims to demonstrate the weaknesses and strengths of using marketing principles in academic libraries and finally presenting some important suggestions in order to improve their marketing. This study proceeds through expressing the current marketing situation of studied academic libraries from the view point of 4P model. In this regard, the current situation is analyzed using a researcher-made questionnaire, and then the problems and shortcomings are highlighted in the field of marketing in the view point of 4P model which in turn enables us to introduce suggestions to overcome them. This research indicates that marketing principles in the studied libraries are not in a good state. The situation of product dimension is a bit farther up middle state (3.23. Also, the Place dimension with total average of 2.97, Promotion dimension with total average of 2.63 and finally the Price dimension with total average of 1.59 are below the middle state. Therefore, trying to make a good plan in marketing in the four mentioned dimensions is essential for studied academic libraries. Improving academic libraries' statement needs identifying their current situation in the view point of marketing. Consequently, this research studied the current situation of Isfahan governmental academic libraries in order to identify their weaknesses and suggest sufficient suggestions to cover them.

  19. Damsel: A Data Model Storage Library for Exascale Science

    Energy Technology Data Exchange (ETDEWEB)

    Koziol, Quincey [The HDF Group

    2014-11-26

    The goal of this project is to enable exascale computational science applications to interact conveniently and efficiently with storage through abstractions that match their data models. We will accomplish this through three major activities: (1) identifying major data model motifs in computational science applications and developing representative benchmarks; (2) developing a data model storage library, called Damsel, that supports these motifs, provides efficient storage data layouts, incorporates optimizations to enable exascale operation, and is tolerant to failures; and (3) productizing Damsel and working with computational scientists to encourage adoption of this library by the scientific community.

  20. Damsel - A Data Model Storage Library for Exascale Science

    Energy Technology Data Exchange (ETDEWEB)

    Samatova, Nagiza F

    2014-07-18

    The goal of this project is to enable exascale computational science applications to interact conveniently and efficiently with storage through abstractions that match their data models. We will accomplish this through three major activities: (1) identifying major data model motifs in computational science applications and developing representative benchmarks; (2) developing a data model storage library, called Damsel, that supports these motifs, provides efficient storage data layouts, incorporates optimizations to enable exascale operation, and is tolerant to failures; and (3) productizing Damsel and working with computational scientists to encourage adoption of this library by the scientific community.

  1. Multiscale Computing with the Multiscale Modeling Library and Runtime Environment

    OpenAIRE

    Borgdorff J.; Mamonski M.; Bosak B.; Groen D.; Ben Belgacem M.; Kurowski K.; Hoekstra A.G.

    2013-01-01

    We introduce a software tool to simulate multiscale models: The Multiscale Coupling Library and Environment 2 (MUSCLE 2). MUSCLE 2 is a component-based modeling tool inspired by the multiscale modeling and simulation framework, with an easy-to-use API which supports Java, C++, C, and Fortran. We present MUSCLE 2's runtime features, such as its distributed computing capabilities, and its benefits to multiscale modelers. We also describe two multiscale models that use MUSCLE 2 to do distributed...

  2. A Model for Library Book Circulations Incorporating Loan Periods.

    Science.gov (United States)

    Burrell, Quentin L.; Fenton, Michael R.

    1994-01-01

    Proposes and explains a modification of the mixed Poisson model for library circulations which takes into account the periods when a book is out on loan and therefore unavailable for borrowing. Highlights include frequency of circulation distributions; negative binomial distribution; and examples of the model at two universities. (Contains 34…

  3. Damsel: A Data Model Storage Library for Exascale Science

    Energy Technology Data Exchange (ETDEWEB)

    Choudhary, Alok [Northwestern Univ., Evanston, IL (United States); Liao, Wei-keng [Northwestern Univ., Evanston, IL (United States)

    2014-07-11

    Computational science applications have been described as having one of seven motifs (the “seven dwarfs”), each having a particular pattern of computation and communication. From a storage and I/O perspective, these applications can also be grouped into a number of data model motifs describing the way data is organized and accessed during simulation, analysis, and visualization. Major storage data models developed in the 1990s, such as Network Common Data Format (netCDF) and Hierarchical Data Format (HDF) projects, created support for more complex data models. Development of both netCDF and HDF5 was influenced by multi-dimensional dataset storage requirements, but their access models and formats were designed with sequential storage in mind (e.g., a POSIX I/O model). Although these and other high-level I/O libraries have had a beneficial impact on large parallel applications, they do not always attain a high percentage of peak I/O performance due to fundamental design limitations, and they do not address the full range of current and future computational science data models. The goal of this project is to enable exascale computational science applications to interact conveniently and efficiently with storage through abstractions that match their data models. The project consists of three major activities: (1) identifying major data model motifs in computational science applications and developing representative benchmarks; (2) developing a data model storage library, called Damsel, that supports these motifs, provides efficient storage data layouts, incorporates optimizations to enable exascale operation, and is tolerant to failures; and (3) productizing Damsel and working with computational scientists to encourage adoption of this library by the scientific community. The product of this project, Damsel library, is openly available for download from http://cucis.ece.northwestern.edu/projects/DAMSEL. Several case studies and application programming interface

  4. Library

    OpenAIRE

    Dulaney, Ronald E. Jr.

    1997-01-01

    This study began with the desire to design a public town library of the future and became a search for an inkling of what is essential to Architecture. It is murky and full of contradictions. It asks more than it proposes, and the traces of its windings are better ordered through collage than logical synthesis. This study is neither a thesis nor a synthesis. When drawing out the measure of this study it may be beneficial to state what it attempts to place at the ...

  5. Towards Automation of ASIC TSMC 0.18 um Standard Cell Library Development

    OpenAIRE

    Djigbenou, Jeannette Donan

    2008-01-01

    Cell-based design is a widely adopted design approach in current Application Specific Integrated Circuits (ASIC) and System-on-Chip (SOC) designs. A standard cell library is a collection of basic building blocks that can be used in cell-based design. The use of a standard cell library offers shorter design time, induces fewer errors in the design process, and is easier to maintain. Development of a cell library is laborious, prone to errors and even a small error on a library cell can poss...

  6. Object-oriented Modular Model Library for Distillation

    Institute of Scientific and Technical Information of China (English)

    CHEN Chang; DING Jianwan; CHEN Liping

    2013-01-01

    For modeling and simulation of distillation process,there are lots of special purpose simulators along with their model libraries,such as Aspen Plus and HYSYS.However,the models in these tools lack of flexibility and are not open to the end-user.Models developed in one tool can not be conveniently used in others because of the barriers among these simulators.In order to solve those problems,a flexible and extensible distillation system model library is constructed in this study,based on the Modelica and Modelica-supported platform MWorks,by the object-oriented technology and level progressive modeling strategy.It supports the reuse of knowledge on different granularities:physical phenomenon,unit model and system model.It is also an interface-friendly,accurate,fast PC-based and easily reusable simulation tool,which enables end-user to customize and extend the framework to add new functionality or adapt the simulation behavior as required.It also allows new models to be composed programmatically or graphically to form more complex models by invoking the existing components.A conventional air distillation column model is built and calculated using the library,and the results agree well with that simulated in Aspen Plus.

  7. Implementing Kuhlthau: A New Model for Library and Reference Instruction.

    Science.gov (United States)

    Isbell, Dennis; Kammerlocher, Lisa

    1998-01-01

    Summarizes Carol Kuhlthau's research on the information search process. Discusses how Kuhlthau's model of students' information search process (ISP) has been integrated into a course at Arizona State University and is being used experimentally as a training tool in the library's reference services. Selected student responses to research process…

  8. The Managerial Roles of Academic Library Directors: The Mintzberg Model.

    Science.gov (United States)

    Moskowitz, Michael Ann

    1986-01-01

    A study based on a model developed by Henry Mintzberg examined the internal and external managerial roles of 126 New England college and university library directors. Survey results indicate that the 97 responding directors were primarily involved with internal managerial roles and work contacts. (CDD)

  9. Sustaining librarian vitality: embedded librarianship model for health sciences libraries.

    Science.gov (United States)

    Wu, Lin; Mi, Misa

    2013-01-01

    With biomedical information widely accessible from anywhere at any time, health sciences libraries have become less centralized, and they are challenged to stay relevant and vital to the mission and strategic goals of their home institution. One solution is to embed librarians at strategic points in health professions' education, research, and patient care. This article discusses a proposed five-level model of embedded librarianship within the context of health sciences libraries and describes different roles, knowledge, and skills desirable for health sciences librarians working as embedded librarians.

  10. Sustaining librarian vitality: embedded librarianship model for health sciences libraries.

    Science.gov (United States)

    Wu, Lin; Mi, Misa

    2013-01-01

    With biomedical information widely accessible from anywhere at any time, health sciences libraries have become less centralized, and they are challenged to stay relevant and vital to the mission and strategic goals of their home institution. One solution is to embed librarians at strategic points in health professions' education, research, and patient care. This article discusses a proposed five-level model of embedded librarianship within the context of health sciences libraries and describes different roles, knowledge, and skills desirable for health sciences librarians working as embedded librarians. PMID:23869633

  11. Mouse protein arrays from a TH1 cell cDNA library for antibody screening and serum profiling

    OpenAIRE

    Gutjahr, C.; Murphy, D.; Lueking, A.; Koenig, A.; Janitz, M; O'Brien, J.; Korn, B. (Bernhard); S. Horn; Lehrach, H; Cahill, D.

    2005-01-01

    The mouse is the premier genetic model organism for the study of disease and development. We describe the establishment of a mouse T helper cell type 1 (TH1) protein expression library that provides direct access to thousands of recombinant mouse proteins, in particular those associated with immune responses. The advantage of a system based on the combination of large cDNA expression libraries with microarray technology is the direct connection of the DNA sequence information from a particula...

  12. Biomass Scenario Model Scenario Library: Definitions, Construction, and Description

    Energy Technology Data Exchange (ETDEWEB)

    Inman, D.; Vimmerstedt, L.; Bush, B.; Peterson, S.

    2014-04-01

    Understanding the development of the biofuels industry in the United States is important to policymakers and industry. The Biomass Scenario Model (BSM) is a system dynamics model of the biomass-to-biofuels system that can be used to explore policy effects on biofuels development. Because of the complexity of the model, as well as the wide range of possible future conditions that affect biofuels industry development, we have not developed a single reference case but instead developed a set of specific scenarios that provide various contexts for our analyses. The purpose of this report is to describe the scenarios that comprise the BSM scenario library. At present, we have the following policy-focused scenarios in our library: minimal policies, ethanol-focused policies, equal access to policies, output-focused policies, technological diversity focused, and the point-of-production- focused. This report describes each scenario, its policy settings, and general insights gained through use of the scenarios in analytic studies.

  13. Jeddah Historical Building Information Modelling "JHBIM" - Object Library

    Science.gov (United States)

    Baik, A.; Alitany, A.; Boehm, J.; Robson, S.

    2014-05-01

    The theory of using Building Information Modelling "BIM" has been used in several Heritage places in the worldwide, in the case of conserving, documenting, managing, and creating full engineering drawings and information. However, one of the most serious issues that facing many experts in order to use the Historical Building Information Modelling "HBIM", is creating the complicated architectural elements of these Historical buildings. In fact, many of these outstanding architectural elements have been designed and created in the site to fit the exact location. Similarly, this issue has been faced the experts in Old Jeddah in order to use the BIM method for Old Jeddah historical Building. Moreover, The Saudi Arabian City has a long history as it contains large number of historic houses and buildings that were built since the 16th century. Furthermore, the BIM model of the historical building in Old Jeddah always take a lot of time, due to the unique of Hijazi architectural elements and no such elements library, which have been took a lot of time to be modelled. This paper will focus on building the Hijazi architectural elements library based on laser scanner and image survey data. This solution will reduce the time to complete the HBIM model and offering in depth and rich digital architectural elements library to be used in any heritage projects in Al-Balad district, Jeddah City.

  14. An event-centric provenance model for digital libraries

    OpenAIRE

    Castelli, Donatella; Candela, Leonardo; Manghi, Paolo; Pagano, Pasquale; Tang, Cristina; Thanos, Costantino

    2010-01-01

    Provenance is intended as the description of the origin and/or of the descendant line of data. In the last decade, keeping track of provenance has become crucial for the correct exploitation of data in a wide variety of application domains. The rapid evolution of digital libraries, which have become today advanced systems for the integration and management of cross-domain digital objects, recently called for models capturing the aspects of data provenance in this application field. However, t...

  15. Stochastic hyperfine interactions modeling library-Version 2

    Science.gov (United States)

    Zacate, Matthew O.; Evenson, William E.

    2016-02-01

    The stochastic hyperfine interactions modeling library (SHIML) provides a set of routines to assist in the development and application of stochastic models of hyperfine interactions. The library provides routines written in the C programming language that (1) read a text description of a model for fluctuating hyperfine fields, (2) set up the Blume matrix, upon which the evolution operator of the system depends, and (3) find the eigenvalues and eigenvectors of the Blume matrix so that theoretical spectra of experimental techniques that measure hyperfine interactions can be calculated. The optimized vector and matrix operations of the BLAS and LAPACK libraries are utilized. The original version of SHIML constructed and solved Blume matrices for methods that measure hyperfine interactions of nuclear probes in a single spin state. Version 2 provides additional support for methods that measure interactions on two different spin states such as Mössbauer spectroscopy and nuclear resonant scattering of synchrotron radiation. Example codes are provided to illustrate the use of SHIML to (1) generate perturbed angular correlation spectra for the special case of polycrystalline samples when anisotropy terms of higher order than A22 can be neglected and (2) generate Mössbauer spectra for polycrystalline samples for pure dipole or pure quadrupole transitions.

  16. Hyperopt: a Python library for model selection and hyperparameter optimization

    Science.gov (United States)

    Bergstra, James; Komer, Brent; Eliasmith, Chris; Yamins, Dan; Cox, David D.

    2015-01-01

    Sequential model-based optimization (also known as Bayesian optimization) is one of the most efficient methods (per function evaluation) of function minimization. This efficiency makes it appropriate for optimizing the hyperparameters of machine learning algorithms that are slow to train. The Hyperopt library provides algorithms and parallelization infrastructure for performing hyperparameter optimization (model selection) in Python. This paper presents an introductory tutorial on the usage of the Hyperopt library, including the description of search spaces, minimization (in serial and parallel), and the analysis of the results collected in the course of minimization. This paper also gives an overview of Hyperopt-Sklearn, a software project that provides automatic algorithm configuration of the Scikit-learn machine learning library. Following Auto-Weka, we take the view that the choice of classifier and even the choice of preprocessing module can be taken together to represent a single large hyperparameter optimization problem. We use Hyperopt to define a search space that encompasses many standard components (e.g. SVM, RF, KNN, PCA, TFIDF) and common patterns of composing them together. We demonstrate, using search algorithms in Hyperopt and standard benchmarking data sets (MNIST, 20-newsgroups, convex shapes), that searching this space is practical and effective. In particular, we improve on best-known scores for the model space for both MNIST and convex shapes. The paper closes with some discussion of ongoing and future work.

  17. Expression sequence tag library derived from peripheral blood mononuclear cells of the chlorocebus sabaeus

    Directory of Open Access Journals (Sweden)

    Tchitchek Nicolas

    2012-06-01

    Full Text Available Abstract Background African Green Monkeys (AGM are amongst the most frequently used nonhuman primate models in clinical and biomedical research, nevertheless only few genomic resources exist for this species. Such information would be essential for the development of dedicated new generation technologies in fundamental and pre-clinical research using this model, and would deliver new insights into primate evolution. Results We have exhaustively sequenced an Expression Sequence Tag (EST library made from a pool of Peripheral Blood Mononuclear Cells from sixteen Chlorocebus sabaeus monkeys. Twelve of them were infected with the Simian Immunodeficiency Virus. The mononuclear cells were or not stimulated in vitro with Concanavalin A, with lipopolysacharrides, or through mixed lymphocyte reaction in order to generate a representative and broad library of expressed sequences in immune cells. We report here 37,787 sequences, which were assembled into 14,410 contigs representing an estimated 12% of the C. sabaeus transcriptome. Using data from primate genome databases, 9,029 assembled sequences from C. sabaeus could be annotated. Sequences have been systematically aligned with ten cDNA references of primate species including Homo sapiens, Pan troglodytes, and Macaca mulatta to identify ortholog transcripts. For 506 transcripts, sequences were quasi-complete. In addition, 6,576 transcript fragments are potentially specific to the C. sabaeus or corresponding to not yet described primate genes. Conclusions The EST library we provide here will prove useful in gene annotation efforts for future sequencing of the African Green Monkey genomes. Furthermore, this library, which particularly well represents immunological and hematological gene expression, will be an important resource for the comparative analysis of gene expression in clinically relevant nonhuman primate and human research.

  18. PNet: A Python Library for Petri Net Modeling and Simulation

    Directory of Open Access Journals (Sweden)

    Zhu En Chay

    2016-07-01

    Full Text Available Petri Net is a formalism to describe changes between 2 or more states across discrete time and has been used to model many systems. We present PNet – a pure Python library for Petri Net modeling and simulation in Python programming language. The design of PNet focuses on reducing the learning curve needed to define a Petri Net by using a text-based language rather than programming constructs to define transition rules. Complex transition rules can be refined as regular Python functions. To demonstrate the simplicity of PNet, we present 2 examples – bread baking, and epidemiological models.

  19. Package PowerTrain: A Modelica library for modeling and simulation of vehicle power trains

    OpenAIRE

    Otter, Martin; Dempsey, Mike; Schlegel, Clemens

    2000-01-01

    In this article a new Modelica library to model vehicle power trains is discussed. An overview of the elements within the library is given, such as planetary gearsets, laminar clutches, torque losses, driver, warm up model. The library is demonstrated through the discussion of two illustrative examples, for drive cycle fuel consumption prediction and for investigating gearshift transition dynamics of an automatic gearbox. Together with the Modelica simulation environment Dymola, this library ...

  20. CMOS Low Power Cell Library for Digital Design

    Directory of Open Access Journals (Sweden)

    Kanika Kaur

    2013-06-01

    Full Text Available Historically, VLSI designers have focused on increasing the speed and reducing the area of digital systems. However, the evolution of portable systems and advanced Deep Sub-Micron fabrication technologies have brought power dissipation as another critical design factor. Low power design reduces cooling cost and increases reliability especially for high density systems. Moreover, it reduces the weight and size of portable devices. The power dissipation in CMOS circuits consists of static and dynamic components. Since dynamic power is proportional to V2 dd and static power is proportional to Vdd, lowering the supply voltage and device dimensions, the transistor threshold voltage also has to be scaled down to achieve the required performance. In case of static power, the power is consumed during the steady state condition i.e when there are no input/output transitions. Static power has two sources: DC power and Leakage power. Consecutively to facilitate voltage scaling without disturbing the performance, threshold voltage has to be minimized. Furthermore it leads to better noise margins and helps to avoid the hot carrier effects in short channel devices. In this paper we have been proposed the new CMOS library for the complex digital design using scaling the supply voltage and device dimensions and also suggest the methods to control the leakage current to obtain the minimum power dissipation at optimum value of supply voltage and transistor threshold. In this paper CMOS Cell library has been implemented using TSMC (0.18um and TSMC (90nm technology using HEP2 tool of IC designing from Mentor Graphics for various analysis and simulations.

  1. OpenGM: A C++ Library for Discrete Graphical Models

    CERN Document Server

    Andres, Bjoern; Kappes, Joerg H

    2012-01-01

    OpenGM is a C++ template library for defining discrete graphical models and performing inference on these models, using a wide range of state-of-the-art algorithms. No restrictions are imposed on the factor graph to allow for higher-order factors and arbitrary neighborhood structures. Large models with repetitive structure are handled efficiently because (i) functions that occur repeatedly need to be stored only once, and (ii) distinct functions can be implemented differently, using different encodings alongside each other in the same model. Several parametric functions (e.g. metrics), sparse and dense value tables are provided and so is an interface for custom C++ code. Algorithms are separated by design from the representation of graphical models and are easily exchangeable. OpenGM, its algorithms, HDF5 file format and command line tools are modular and extendible.

  2. AST: A library for modelling and manipulating coordinate systems

    Science.gov (United States)

    Berry, David S.; Warren-Smith, Rodney F.; Jenness, Tim

    2016-04-01

    In view of increased interest in object-oriented systems for describing coordinate information, we present a description of the data model used by the Starlink AST library. AST provides a comprehensive range of facilities for attaching world co-ordinate systems to astronomical data, and for retrieving and interpreting that information in a variety of formats, including FITS-WCS. AST is a mature system that has been in use for more than 17 years, and may consequently be useful as a means of informing development of similar systems in the future.

  3. AST: A library for modelling and manipulating coordinate systems

    CERN Document Server

    Berry, David; Jenness, Tim

    2016-01-01

    In view of increased interest in object-oriented systems for describing coordinate information, we present a description of the data model used by the Starlink AST library. AST provides a comprehensive range of facilities for attaching world co-ordinate systems to astronomical data, and for retrieving and interpreting that information in a variety of formats, including FITS-WCS. AST is a mature system that has been in use for more than 17 years, and may consequently be useful as a means of informing development of similar systems in the future.

  4. Establishing a Selection Process Model for an Ethnic Collection in a Prison Library.

    Science.gov (United States)

    Haymann-Diaz, Barbara

    1989-01-01

    Describes a study that examined the selection process used by two inmate/library assistants to develop Hispanic and African ethnic collections in a prison library. The findings of the study are used to develop a selection process model for the development of ethnic collections using the expertise of ethnic inmate/library assistants. (25…

  5. A Web-Based Electronic Book (e-book) Library: The netLibrary Model.

    Science.gov (United States)

    Connaway, Lynn Silipigni

    2001-01-01

    Identifies elements that are important for academic libraries to use in evaluating electronic books, including content; acquisition and collection development; software and hardware standards and protocols; digital rights management; access; archiving; privacy; the market and pricing; and enhancements and ideal features. Describes netLibrary, a…

  6. A computational library for multiscale modeling of material failure

    Science.gov (United States)

    Talebi, Hossein; Silani, Mohammad; Bordas, Stéphane P. A.; Kerfriden, Pierre; Rabczuk, Timon

    2014-05-01

    We present an open-source software framework called PERMIX for multiscale modeling and simulation of fracture in solids. The framework is an object oriented open-source effort written primarily in Fortran 2003 standard with Fortran/C++ interfaces to a number of other libraries such as LAMMPS, ABAQUS, LS-DYNA and GMSH. Fracture on the continuum level is modeled by the extended finite element method (XFEM). Using several novel or state of the art methods, the piece software handles semi-concurrent multiscale methods as well as concurrent multiscale methods for fracture, coupling two continuum domains or atomistic domains to continuum domains, respectively. The efficiency of our open-source software is shown through several simulations including a 3D crack modeling in clay nanocomposites, a semi-concurrent FE-FE coupling, a 3D Arlequin multiscale example and an MD-XFEM coupling for dynamic crack propagation.

  7. A new adaptive data transfer library for model coupling

    Science.gov (United States)

    Zhang, Cheng; Liu, Li; Yang, Guangwen; Li, Ruizhe; Wang, Bin

    2016-06-01

    Data transfer means transferring data fields from a sender to a receiver. It is a fundamental and frequently used operation of a coupler. Most versions of state-of-the-art couplers currently use an implementation based on the point-to-point (P2P) communication of the message passing interface (MPI) (referred to as "P2P implementation" hereafter). In this paper, we reveal the drawbacks of the P2P implementation when the parallel decompositions of the sender and the receiver are different, including low communication bandwidth due to small message size, variable and high number of MPI messages, as well as network contention. To overcome these drawbacks, we propose a butterfly implementation for data transfer. Although the butterfly implementation outperforms the P2P implementation in many cases, it degrades the performance when the sender and the receiver have similar parallel decompositions or when the number of processes used for running models is small. To ensure data transfer with optimal performance, we design and implement an adaptive data transfer library that combines the advantages of both butterfly implementation and P2P implementation. As the adaptive data transfer library automatically uses the best implementation for data transfer, it outperforms the P2P implementation in many cases while it does not decrease the performance in any cases. Now, the adaptive data transfer library is open to the public and has been imported into the C-Coupler1 coupler for performance improvement of data transfer. We believe that other couplers can also benefit from this.

  8. Rock.XML - Towards a library of rock physics models

    Science.gov (United States)

    Jensen, Erling Hugo; Hauge, Ragnar; Ulvmoen, Marit; Johansen, Tor Arne; Drottning, Åsmund

    2016-08-01

    Rock physics modelling provides tools for correlating physical properties of rocks and their constituents to the geophysical observations we measure on a larger scale. Many different theoretical and empirical models exist, to cover the range of different types of rocks. However, upon reviewing these, we see that they are all built around a few main concepts. Based on this observation, we propose a format for digitally storing the specifications for rock physics models which we have named Rock.XML. It does not only contain data about the various constituents, but also the theories and how they are used to combine these building blocks to make a representative model for a particular rock. The format is based on the Extensible Markup Language XML, making it flexible enough to handle complex models as well as scalable towards extending it with new theories and models. This technology has great advantages as far as documenting and exchanging models in an unambiguous way between people and between software. Rock.XML can become a platform for creating a library of rock physics models; making them more accessible to everyone.

  9. E-Books and New Library Service Models: An Analysis of the Impact of E-Book Technology on Academic Libraries.

    Science.gov (United States)

    Jantz, Ronald

    2001-01-01

    Analyzes the implications of electronic book technology (e-books) on academic libraries. Discusses new business models for publishers, including self-publishing, Internet publishing, and partnerships with libraries as publishers; impact on library services, including cataloging, circulation, and digital preservation; user benefits; standards;…

  10. Scanning electrochemical microscope characterization of thin film combinatorial libraries for fuel cell electrode applications

    Science.gov (United States)

    Black, M.; Cooper, J.; McGinn, P.

    2005-01-01

    Pt-Ru combinatorial libraries of potential fuel cell anode catalysts are formed by sequential sputter deposition through masks onto Si wafers. Scanning electrochemical microscopy (SECM) is employed for characterization of electrocatalytic activity. Aspects of using a scanning electrochemical microscope for characterization of an array of thin film fuel cell electrode materials are discussed. It is shown that in applying SECM to library characterization, careful attention must be paid to thin film annealing, specimen topography and tip degradation in order to realize meaningful results. Results from a Pt-Ru thin film library reveal the most active members near the 50 Pt/50 Ru composition.

  11. SRADLIB: A C Library for Solar Radiation Modelling

    Energy Technology Data Exchange (ETDEWEB)

    Balenzategui, J. L. [Ciemat. Madrid (Spain)

    2000-07-01

    This document shows the result of an exhaustive study about the theoretical and numerical models available in the literature about solar radiation modelling. The purpose of this study is to develop or adapt mathematical models describing the solar radiation specifically for Spain locations as well as to create computer tools able to support the labour of researchers or engineers needing solar radiation data to solve or improve the technical or energetic performance of solar systems. As results of this study and revision, a C library (SRADLIB) is presented as a key for the compilation of the mathematical models from different authors, for the comparison among the different approaches and for its application in computer programs. Different topics related to solar radiation and its modelling are first discussed, including the assumptions and conventions adopted and describing the most accepted and used current state-of-the-art models. some typical problems in the numerical calculation of radiation values are also posed with the proposed solution. The document includes next a complete reference of the developed functions, with many examples of application and calculus. (Author) 24 refs.

  12. A Third Note on Ageing in a Library Circulation Model: Applications to Future Use and Relegation.

    Science.gov (United States)

    Burrell, Quentin L.

    1987-01-01

    Describes a circulation model for academic research libraries which uses the mixed Poisson model, incorporating ageing of library materials, to predict future use of monographs and to suggest weeding procedures based on frequency of circulation. Longitudinal studies are examined and statistical details are appended. (Author/LRW)

  13. Evolution of Reference: A New Service Model for Science and Engineering Libraries

    Science.gov (United States)

    Bracke, Marianne Stowell; Chinnaswamy, Sainath; Kline, Elizabeth

    2008-01-01

    This article explores the different steps involved in adopting a new service model at the University of Arizona Science-Engineering Library. In a time of shrinking budgets and changing user behavior the library was forced to rethink it reference services to be cost effective and provide quality service at the same time. The new model required…

  14. Cost of Electronic Reference Resources and LCM: The Library Costing Model.

    Science.gov (United States)

    Hayes, Robert M.

    1996-01-01

    Views assessment of "Costs of Electronic Reference Resources" both in a general descriptive framework and within the context of a specific model for costing of library operations and services called LCM, the Library Costing Model. Examples of costing data uses are provided, and categories of costs are related to types of electronic reference…

  15. A Model for Designing Library Instruction for Distance Learning

    Science.gov (United States)

    Rand, Angela Doucet

    2013-01-01

    Providing library instruction in distance learning environments presents a unique set of challenges for instructional librarians. Innovations in computer-mediated communication and advances in cognitive science research provide the opportunity for designing library instruction that meets a variety of student information seeking needs. Using a…

  16. Building Component Library: An Online Repository to Facilitate Building Energy Model Creation; Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Fleming, K.; Long, N.; Swindler, A.

    2012-05-01

    This paper describes the Building Component Library (BCL), the U.S. Department of Energy's (DOE) online repository of building components that can be directly used to create energy models. This comprehensive, searchable library consists of components and measures as well as the metadata which describes them. The library is also designed to allow contributors to easily add new components, providing a continuously growing, standardized list of components for users to draw upon.

  17. Assessment of a 42 metal salts chemical library in mouse embryonic stem cells

    Science.gov (United States)

    The developmental effects of xenobiotics on differentiation can be profiled using mouse embryonic stem cells (mESCs). The adherent cell differentiation and cytotoxicity (ACDC) technique was used to evaluate a library of 42 metal and metaloid salts. Jl mESCs were allowed to prolif...

  18. Great Service Pays: A Model for Service Delivery in an Academic Music Library

    Science.gov (United States)

    Wilson, Andrew M.

    2007-01-01

    Special-subject libraries can be particularly intimidating for casual and seasoned patrons alike. Music libraries, with their variety of materials, formats, and vocabularies, can present particular challenges for the user. With this proposal for a model of service delivery, as well as many tips gained through experience working the front-of-house…

  19. Equalizing Access to Electronic Networked Resources: A Model for Rural Libraries in the United States.

    Science.gov (United States)

    Senkevitch, Judith J.; Wolfram, Dietmar

    1994-01-01

    Provides an overview of the current state of networking technology in rural libraries and describes a model for educating rural librarians in accessing electronic networks. Topics discussed include information needs in rural libraries; telecommunications technology access in rural areas; and examples of services to enhance information access.…

  20. Beta Spaces as a Model for Recontextualizing Reference Services in Libraries

    OpenAIRE

    Madelynn Dickerson

    2016-01-01

    In Brief: Reference services are at a cross-roads. While many academic libraries continue to offer reference services from behind a desk, others are moving to roving and embedded reference models. Meanwhile, libraries are also engaged in the development of collaborative learning spaces—often rich with technology, such as makerspaces and learning labs—but these spaces are often […

  1. Digital Libraries: Analysis of Delos Reference Model and 5S Theory

    Directory of Open Access Journals (Sweden)

    Isah, Abdulmumin

    2013-12-01

    Full Text Available The proliferation of digital libraries (DL in the twenty-first century has revolutionized the way information is generated and disseminated. This has led to various practical and research models of DLs. This paper discusses the concept and development of digital libraries, and examines various components and characteristics of DLs. It further identifies various models and theories of digital libraries with a special focus on the DELOS Reference Model and 5S Theory. The relationship between the two focused frameworks is analyzed for better understanding of their application in the DL universe.

  2. The Role of Libraries in Emerging Models of Scholarly Communication

    OpenAIRE

    Jottkandt, Sigi; Willinsky, John; Kimball, Shana

    2009-01-01

    Will transformations in technology, media and scholarly cultures lead to the declining importance or ‘irrelevance’ of the university library, or can these changes be envisioned in terms of what cultural critic Ajit Pyati has called a greater “democratic participation of libraries”?1 By this, Pyati has in mind the library as an “active shaper” of technology for the progressive end of increased information access for all. Integral to this vision is an expansion rather than contraction in librar...

  3. The National Resource Library for Science and Technology in Sweden: A Nordic model of cooperating technology libraries

    Science.gov (United States)

    Lagar, Gunnar

    1994-01-01

    The scope of this presentation is to give a state-of-the-art report on the present situation of Nordic technology libraries, to elaborate on a plan for national resource libraries in Sweden, and to share how the Royal Institute of Technology Library in Stockholm (KTHB) has fostered a network of cooperating libraries in order to optimize government funding for the system of resource libraries.

  4. Models of Reference Services in Australian Academic Libraries

    Science.gov (United States)

    Burke, Liz

    2008-01-01

    This article reports on a project which was undertaken in 2006 to investigate the current modes and methods for delivering reference services in Australian academic libraries. The project included a literature review to assist in providing a definition of reference services as well as a snapshot of statistics showing staff and patron numbers from…

  5. Human Factors of Queuing: A Library Circulation Model.

    Science.gov (United States)

    Mansfield, Jerry W.

    1981-01-01

    Classical queuing theories and their accompanying service facilities totally disregard the human factors in the name of efficiency. As library managers we need to be more responsive to human needs in the design of service points and make every effort to minimize queuing and queue frustration. Five references are listed. (Author/RAA)

  6. An Analytical Model for Service Profile Based Service Quality of an Institutional eLibrary

    CERN Document Server

    Abbas, Ash Mohammad

    2011-01-01

    Devising a scheme for evaluating the service quality of an institutional electronic library is a difficult and challenging task. The challenge comes from the fact that the services provided by an institutional electronic library depend upon the contents requested by the users and the contents housed by the library. Different types of users might be interested in different types of contents. In this paper, we propose a technique for evaluating the service quality of an institutional electronic library. Our scheme is based on the service profiles of contents requested by the users at the server side which is hosted at the library. Further, we propose models to analyze the service quality of an electronic library. For analyzing the service quality, we present two analytical models. The first one is based on the number of days by which the item to be served by the library is delayed and the penalty points per day for the duration for which the item is delayed. The second model is based on the credits earned by th...

  7. A New Model and Its Implementation of Academic Resources Services for Academic Libraries

    Directory of Open Access Journals (Sweden)

    Yu-Ting Chiang

    2006-12-01

    Full Text Available Internet has become one of the important channels for retrieval of academic resources in the recent years. The roles and service models of academic libraries has been changed accordingly. Most academic libraries have been providing subject directory (subject gateway for users to browse the highly selected academic resources. On the contrary, few academic libraries managed to provide academic resources for user to retrieve. This article proposes a new model which the libraries will cooperate with search engine providers to support the full-text retrieval for academic resources in a low cost. We discuss the design and implementation of “NTU Scholars Gateway” based on the new model. In addition, the functionalities and future development of NTU Scholars Gateway are discussedas well. [Article content in Chinese

  8. An efficient strategy for cell-based antibody library selection using an integrated vector system

    Directory of Open Access Journals (Sweden)

    Yoon Hyerim

    2012-09-01

    Full Text Available Abstract Background Cell panning of phage-displayed antibody library is a powerful tool for the development of therapeutic and imaging agents since disease-related cell surface proteins in native complex conformation can be directly targeted. Here, we employed a strategy taking advantage of an integrated vector system which allows rapid conversion of scFv-displaying phage into scFv-Fc format for efficient cell-based scFv library selection on a tetraspanin protein, CD9. Results A mouse scFv library constructed by using a phagemid vector, pDR-D1 was subjected to cell panning against stable CD9 transfectant, and the scFv repertoire from the enriched phage pool was directly transferred to a mammalian cassette vector, pDR-OriP-Fc1. The resulting constructs enabled transient expression of enough amounts of scFv-Fcs in HEK293E cells, and flow cytometric screening of binders for CD9 transfectant could be performed simply by using the culture supernatants. All three clones selected from the screening showed correct CD9-specificity. They could immunoprecipitate CD9 molecules out of the transfectant cell lysate and correctly stain endogenous CD9 expression on cancer cell membrane. Furthermore, competition assay with a known anti-CD9 monoclonal antibody (mAb suggested that the binding epitopes of some of them overlap with that of the mAb which resides within the large extracellular loop of CD9. Conclusions This study demonstrates that scFv-Fc from mammalian transient expression can be chosen as a reliable format for rapid screening and validation in cell-based scFv library selection, and the strategy described here will be applicable to efficient discovery of antibodies to diverse cell-surface targets.

  9. Mining a Yeast Library for Brain Endothelial Cell-Binding Antibodies

    OpenAIRE

    Wang, Xin Xiang; Cho, Yong Ku; Shusta, Eric V.

    2007-01-01

    We describe the use of yeast surface display for the identification of antibodies that bind the plasma membranes of living cells. Yeast panning with a nonimmune human single-chain antibody library identified 34 unique lead antibodies that bind (Kd = 82 ± 15 nM) and in some cases internalize into rat brain endothelial cells. In addition, a novel yeast display immunoprecipitation procedure was employed for initial characterization of the cognate antigens.

  10. 图书馆系统软件评价模型%Software Evaluation Model for Libraries

    Institute of Scientific and Technical Information of China (English)

    潘英; 丁晶

    2000-01-01

    The paper deals with the assessment of software products for libraries'computers before libraries purchase software products for documentary information management. Based on the Analytic Hierarchy Process, and according to the general evaluation criterions, an analytic hierarchy model is used to evaluate three computer software products for libraries.

  11. The American Library Association in Latin America: American Librarianship as a "Modern" Model during the Good Neighbor Policy Era.

    Science.gov (United States)

    Maymi-Sugranes, Hector J.

    2002-01-01

    Through American Library Association (ALA) projects in Latin America, American librarianship progressed from conceptualization to implementation as the model in modernizing Latin American library practices and societies. Development of library practices was fundamental to pursuit of a "modern" society. In fighting fascist propaganda, the United…

  12. Portuguese School Libraries Evaluation Model: An Analysis of Primary Schools' Results for the "Reading and Literacy" Domain

    Science.gov (United States)

    Martins, Jorge Tiago; Martins, Rosa Maria

    2012-01-01

    This paper reports the implementation results of the Portuguese School Libraries Evaluation Model, more specifically the results of primary schools self-evaluation of their libraries' reading promotion and information literacy development activities. School libraries that rated their performance as either "Excellent" or "Poor" have been…

  13. Beta Spaces as a Model for Recontextualizing Reference Services in Libraries

    Directory of Open Access Journals (Sweden)

    Madelynn Dickerson

    2016-05-01

    Full Text Available In Brief: Reference services are at a cross-roads. While many academic libraries continue to offer reference services from behind a desk, others are moving to roving and embedded reference models. Meanwhile, libraries are also engaged in the development of collaborative learning spaces—often rich with technology, such as makerspaces and learning labs—but these spaces are often […

  14. Supporting Teaching and Research in an Online Environment: Developing the University of London Library Model

    Directory of Open Access Journals (Sweden)

    Sally Chambers

    2002-04-01

    Full Text Available Founded in 1837, the University of London Library (ULL is the central research library of the University of London with particular research strengths across the broader arts, humanities and social sciences. With the development of information and communications technology, it has become a hybrid library, offering access to electronic collections but also extending access to services and its physical collections through its website and online catalogue. Research libraries now face the challenge of supporting distance learning. The ULL has risen to this challenge by being a partner in the development of webenhanced distance learning by the University of London External Programme’s Virtual Campus Project. To do this, the ULL has initiated the Virtual Library Service (VLS Project. Within the research-focussed University of London, the ULL has anticipated the potential research-support needs of its students. It is also leading a separately funded Virtual Research Environment (VRE Project on behalf of the University Libraries Committee to address these needs and inform the University of London’s activities in the virtual sphere. The University of London has created the Electronic Library Projects Team (ELPT to undertake the VLS and VRE projects and ensure that they develop along complementary lines. This paper outlines how the ULL is supporting teaching and research in an online environment through the development of its VLS and VRE models.

  15. Discovery of T cell antigens by high-throughput screening of synthetic minigene libraries.

    Directory of Open Access Journals (Sweden)

    Brian D Hondowicz

    Full Text Available The identification of novel T cell antigens is central to basic and translational research in autoimmunity, tumor immunology, transplant immunology, and vaccine design for infectious disease. However, current methods for T cell antigen discovery are low throughput, and fail to explore a wide range of potential antigen-receptor interactions. To overcome these limitations, we developed a method in which programmable microarrays are used to cost-effectively synthesize complex libraries of thousands of minigenes that collectively encode the content of hundreds of candidate protein targets. Minigene-derived mRNA are transfected into autologous antigen presenting cells and used to challenge complex populations of purified peripheral blood CD8+ T cells in multiplex, parallel ELISPOT assays. In this proof-of-concept study, we apply synthetic minigene screening to identify two novel pancreatic islet autoantigens targeted in a patient with Type I Diabetes. To our knowledge, this is the first successful screen of a highly complex, synthetic minigene library for identification of a T cell antigen. In principle, responses against the full protein complement of any tissue or pathogen can be assayed by this approach, suggesting that further optimization of synthetic libraries holds promise for high throughput antigen discovery.

  16. ModFossa: A library for modeling ion channels using Python.

    Science.gov (United States)

    Ferneyhough, Gareth B; Thibealut, Corey M; Dascalu, Sergiu M; Harris, Frederick C

    2016-06-01

    The creation and simulation of ion channel models using continuous-time Markov processes is a powerful and well-used tool in the field of electrophysiology and ion channel research. While several software packages exist for the purpose of ion channel modeling, most are GUI based, and none are available as a Python library. In an attempt to provide an easy-to-use, yet powerful Markov model-based ion channel simulator, we have developed ModFossa, a Python library supporting easy model creation and stimulus definition, complete with a fast numerical solver, and attractive vector graphics plotting. PMID:26932271

  17. Implementation of integral viscoelastic constitutive models in OpenFOAMxAE computational library

    Science.gov (United States)

    de Araújo, Manuel Silvino Batalha; Fernandes, Célio; Ferrás, Luís Lima; Tukovic, Željko; Jasak, Hrvoje; Nóbrega, João Miguel

    2015-04-01

    This work reports the implementation and verification of a new solver in OpenFOAM® open source computational library, able to cope with integral viscoelastic models based on the integral upper-convected Maxwell model. The code is verified through the comparison of its predictions with analytical solutions and numerical results obtained with the differential upper-convected Maxwell model.

  18. Library Use

    DEFF Research Database (Denmark)

    Konzack, Lars

    2012-01-01

    A seminar paper about a survey of role-playing games in public libraries combined with three cases and a presentation of a model.......A seminar paper about a survey of role-playing games in public libraries combined with three cases and a presentation of a model....

  19. Neutron Capture gamma ENDF libraries for modeling and identification of neutron sources

    International Nuclear Information System (INIS)

    There are a number of inaccuracies and data omissions with respect to gammas from neutron capture in the ENDF libraries used as field reference information and by modeling codes used in JTOT. As the use of Active Neutron interrogation methods is expanded, these shortfalls become more acute. A new, more accurate and complete evaluated experimental database of gamma rays (over 35,000 lines for 262 isotopes up to U so far) from thermal neutron capture has recently become available from the IAEA. To my knowledge, none of this new data has been installed in ENDF libraries and disseminated. I propose to upgrade libraries of 184,186W, 56Fe, 204,206,207Pb, 104Pd, and 19F the 1st year. This will involve collaboration with Richard Firestone at LBL in evaluating the data and installing it in the libraries. I will test them with the transport code MCNP5

  20. Simulink library project for modeling and simulation of dynamic phenomena in rotating power transmission systems

    Directory of Open Access Journals (Sweden)

    Tomasz MATYJA

    2014-06-01

    Full Text Available This paper presents the concept and an example of usage of Simulink blocks library with which dynamic simulation of complex systems with rotating shafts, rigid rotors, bearings and couplings, general rotating power transmission systems of any configuration can be performed. The assumption is that library is modular and expandable. The main part of the library currently being developed is rigid rotor model with 6 degrees of freedom of the static and dynamic imbalance. Other components are: block modeling the bearing with mounting stiffness, damping and inertia; linear elastic-damping element and rigid beam finite element (RFEM. Also in preparation are: block modeling shaft with Timoshenko beam elements and Rayleigh damping, block modeling clutch.

  1. Online Library of Scientific Models, A New Way to Teach, Learn, and Share Learning Experience

    Directory of Open Access Journals (Sweden)

    Hatem H. Elrefaei

    2008-05-01

    Full Text Available While scientific models are usually communicated in paper format, the need to reprogram every model by every user results in a huge loss of efforts, time and money, hence lengthening the educational and research developing cycle and loosing the learning experience and expertise gained by every user. We demonstrate a new portal www.imodelit.com that hosts a library of scientific models for electrical engineers in the form of java applets. They are all conformal, informative, with strong input and output filing system. The software design allows a fast developing cycle and it represents a strong infrastructure that can be shared by researchers to develop their own applets to be posted on the library. We aim for a community based library of scientific models that enhances the e-learning process for engineering students.

  2. A Journey Worth Taking: Exploring a Hybrid Embedded Library Instruction Model Through Three Distinct Cases.

    Science.gov (United States)

    Clifton, Shari; Jo, Phill

    2016-01-01

    To facilitate systematic learning and to complement the limitations of conventional one-shot library instruction sessions, a hybrid embedded instruction model was designed and implemented for undergraduate students and residents in three disciplines at the University of Oklahoma Health Sciences Center. Variations of hybrid instruction are explored, including models that combine face-to-face interactions, online content delivery, flipped instruction techniques, and individual consultations. The hybrid model highlights benefits of collaborative teaching between course faculty members and librarians and enhances the relevance of library instruction for users. PMID:27391181

  3. Synthesis and cell-free cloning of DNA libraries using programmable microfluidics.

    Science.gov (United States)

    Ben Yehezkel, Tuval; Rival, Arnaud; Raz, Ofir; Cohen, Rafael; Marx, Zipora; Camara, Miguel; Dubern, Jean-Frédéric; Koch, Birgit; Heeb, Stephan; Krasnogor, Natalio; Delattre, Cyril; Shapiro, Ehud

    2016-02-29

    Microfluidics may revolutionize our ability to write synthetic DNA by addressing several fundamental limitations associated with generating novel genetic constructs. Here we report the first de novo synthesis and cell-free cloning of custom DNA libraries in sub-microliter reaction droplets using programmable digital microfluidics. Specifically, we developed Programmable Order Polymerization (POP), Microfluidic Combinatorial Assembly of DNA (M-CAD) and Microfluidic In-vitro Cloning (MIC) and applied them to de novo synthesis, combinatorial assembly and cell-free cloning of genes, respectively. Proof-of-concept for these methods was demonstrated by programming an autonomous microfluidic system to construct and clone libraries of yeast ribosome binding sites and bacterial Azurine, which were then retrieved in individual droplets and validated. The ability to rapidly and robustly generate designer DNA molecules in an autonomous manner should have wide application in biological research and development. PMID:26481354

  4. Synthesis and cell-free cloning of DNA libraries using programmable microfluidics

    Science.gov (United States)

    Yehezkel, Tuval Ben; Rival, Arnaud; Raz, Ofir; Cohen, Rafael; Marx, Zipora; Camara, Miguel; Dubern, Jean-Frédéric; Koch, Birgit; Heeb, Stephan; Krasnogor, Natalio; Delattre, Cyril; Shapiro, Ehud

    2016-01-01

    Microfluidics may revolutionize our ability to write synthetic DNA by addressing several fundamental limitations associated with generating novel genetic constructs. Here we report the first de novo synthesis and cell-free cloning of custom DNA libraries in sub-microliter reaction droplets using programmable digital microfluidics. Specifically, we developed Programmable Order Polymerization (POP), Microfluidic Combinatorial Assembly of DNA (M-CAD) and Microfluidic In-vitro Cloning (MIC) and applied them to de novo synthesis, combinatorial assembly and cell-free cloning of genes, respectively. Proof-of-concept for these methods was demonstrated by programming an autonomous microfluidic system to construct and clone libraries of yeast ribosome binding sites and bacterial Azurine, which were then retrieved in individual droplets and validated. The ability to rapidly and robustly generate designer DNA molecules in an autonomous manner should have wide application in biological research and development. PMID:26481354

  5. Antibodies to human fetal erythroid cells from a nonimmune phage antibody library

    OpenAIRE

    Huie, Michael A.; Cheung, Mei-Chi; Muench, Marcus O.; Becerril, Baltazar; Kan, Yuet W.; Marks, James D.

    2001-01-01

    The ability to isolate fetal nucleated red blood cells (NRBCs) from the maternal circulation makes possible prenatal genetic analysis without the need for diagnostic procedures that are invasive for the fetus. Such isolation requires antibodies specific to fetal NRBCs. To generate a panel of antibodies to antigens present on fetal NRBCs, a new type of nonimmune phage antibody library was generated in which multiple copies of antibody fragments are displayed on each pha...

  6. Synthesis and cell-free cloning of DNA libraries using programmable microfluidics

    OpenAIRE

    Yehezkel, Tuval Ben; Rival, Arnaud; Raz, Ofir; Cohen, Rafael; Marx, Zipora; Camara, Miguel; Dubern, Jean-Frédéric; Koch, Birgit; Heeb, Stephan; Krasnogor, Natalio; Delattre, Cyril; Shapiro, Ehud

    2015-01-01

    Microfluidics may revolutionize our ability to write synthetic DNA by addressing several fundamental limitations associated with generating novel genetic constructs. Here we report the first de novo synthesis and cell-free cloning of custom DNA libraries in sub-microliter reaction droplets using programmable digital microfluidics. Specifically, we developed Programmable Order Polymerization (POP), Microfluidic Combinatorial Assembly of DNA (M-CAD) and Microfluidic In-vitro Cloning (MIC) and a...

  7. Evaluation of a Digital Library by Means of Quality Function Deployment (QFD) and the Kano Model

    Science.gov (United States)

    Garibay, Cecilia; Gutierrez, Humberto; Figueroa, Arturo

    2010-01-01

    This paper proposes utilizing a combination of the Quality Function Deployment (QFD)-Kano model as a useful tool to evaluate service quality. The digital library of the University of Guadalajara (Mexico) is presented as a case study. Data to feed the QFD-Kano model was gathered by an online questionnaire that was made available to users on the…

  8. Preliminary assessment of Geant4 HP models and cross section libraries by reactor criticality benchmark calculations

    DEFF Research Database (Denmark)

    Cai, Xiao-Xiao; Llamas-Jansa, Isabel; Mullet, Steven;

    2013-01-01

    Geant4 is an open source general purpose simulation toolkit for particle transportation in matter. Since the extension of the thermal scattering model in Geant4.9.5 and the availability of the IAEA HP model cross section libraries, it is now possible to extend the application area of Geant4 to re...

  9. An Exploratory Study to Develop an Alternative Model of Public Library Management Using the Institute of Museum and Library Services' Public Library Statistics

    Science.gov (United States)

    Kim, Giyeong; Yu, So Young

    2011-01-01

    In this explorative study, we first investigate current use of public library statistics in public library management to identify a governing framework and then carefully suggest an alternative framework with income as a goal for sustainability. The meaning of income in terms of management is also discussed. Within this framework, we conduct a…

  10. From activity to learning: using cultural historical activity theory to model school library programmes and practices

    Directory of Open Access Journals (Sweden)

    E.M. Meyers

    2007-01-01

    Full Text Available Introduction. changes in educational policy and practice demand that we examine school library programmes from a new perspective. As a model that takes a developmental view of minds in context, Cultural Historical Activity Theory is particularly well suited to the study of school libraries and the learning that occurs therein. This paper focuses on the activity theoretic concepts of 'contradictions' and 'expansive learning' as they relate to the development of best practices. Method. Developmental Work Research was applied as a guiding methodology in an intervention study of six high school libraries in Washington State, USA. Library activity is illustrated from multiple perspectives using a triangulated, qualitative approach. Analysis. contradictions and tensions in the general school library activity system are identified, and an intervention was designed to facilitiate the development of expansive instruments. A case example illustrates a second level of analysis and specific points of intervention. Results. analysis reveals that the tensions and contradictions provide opportunities for expansive learning on the part of the teacher-Librarians. The research team can use practitioners' zones of proximal development to guide the alignment of library programme goals and practices. Conclusion. : some limitations and future promise of the framework and its application are discussed. The proposed activity theory toolkit suggest a new way of exploring the practices of teacher-librarians, incorporating research evidence, professional expertise, and reflective decision making.

  11. Linking Formal and Informal Science Education: A Successful Model using Libraries, Volunteers and NASA Resources

    Science.gov (United States)

    Race, M. S.; Lafayette Library; Learning Center Foundation (Lllcf)

    2011-12-01

    In these times of budget cuts, tight school schedules, and limited opportunities for student field trips and teacher professional development, it is especially difficult to expose elementary and middle school students to the latest STEM information-particularly in the space sciences. Using our library as a facilitator and catalyst, we built a volunteer-based, multi-faceted, curriculum-linked program for students and teachers in local middle schools (Grade 8) and showcased new astronomical and planetary science information using mainly NASA resources and volunteer effort. The project began with the idea of bringing free NASA photo exhibits (FETTU) to the Lafayette and Antioch Libraries for public display. Subsequently, the effort expanded by adding layers of activities that brought space and science information to teachers, students and the pubic at 5 libraries and schools in the 2 cities, one of which serves a diverse, underserved community. Overall, the effort (supported by a pilot grant from the Bechtel Foundation) included school and library based teacher workshops with resource materials; travelling space museum visits with hands-on activities (Chabot-to-Go); separate powerpoint presentations for students and adults at the library; and concurrent ancillary space-related themes for young children's programs at the library. This pilot project, based largely on the use of free government resources and online materials, demonstrated that volunteer-based, standards-linked STEM efforts can enhance curriculum at the middle school, with libraries serving a special role. Using this model, we subsequently also obtained a small NASA-Space Grant award to bring star parties and hand-on science activities to three libraries this Fall, linking with numerous Grade 5 teachers and students in two additional underserved areas of our county. It's not necessary to reinvent the wheel, you just collect the pieces and build on what you already have.

  12. Two agricultural production data libraries for risk assessment models. [Ovis aries; Capra hircus; Sus scrofa; Gallus domesticus; Meleagris gallopavo

    Energy Technology Data Exchange (ETDEWEB)

    Baes, C.F. III; Shor, R.W.; Sharp, R.D.; Sjoreen, A.L.

    1985-01-01

    Two data libraries based on the 1974 US Census of Agriculture are described. The data packages (AGDATC and AGDATG) are available from the Radiation Shielding Information Center (RSIC), Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831. Agricultural production and land-use information by county (AGDATC) or by 1/2 by 1/2 degree longitude-latitude grid cell (AGDATG) provide geographical resolution of the data. The libraries were designed for use in risk assessment models that simulate the transport of radionuclides from sources of airborne release through food chains to man. However, they are also suitable for use in the assessment of other airborne pollutants that can affect man from a food ingestion pathway such as effluents from synfuels or coal-fired power plants. The principal significance of the data libraries is that they provide default location-specific food-chain transport parameters when site-specific information are unavailable. Plant food categories in the data libraries include leafy vegetables, vegetables and fruits exposed to direct deposition of airborne pollutants, vegetables and fruits protected from direct deposition, and grains. Livestock feeds are also tabulated in four categories: pasture, grain, hay, and silage. Pasture was estimated by a material balance of cattle and sheep inventories, forage feed requirements, and reported harvested forage. Cattle (Bos spp.), sheep (Ovis aries), goat (Capra hircus), hog (Sus scrofa), chicken (Gallus domesticus), and turkey (Meleagris gallopavo) inventories or sales are also tabulated in the data libraries and can be used to provide estimates of meat, eggs, and milk production. Honey production also is given. Population, irrigation, and meteorological information are also listed.

  13. Library Liaison with non-academic units: a new application for a traditional model

    Directory of Open Access Journals (Sweden)

    Candice C Dahl

    2007-05-01

    Full Text Available This paper suggests that traditional models of liaison librarianship, which focus on liaison with academic units such as teaching departments and specific colleges within institutions, should be adapted and then expanded to include non-academic units as well. Although the literature demonstrates that understandings of liaison work have evolved, it still contains almost no examples of how to extend library liaison beyond traditional academic units in a systematic way. As a result, existing liaison programs are too narrowly conceived, and the strengths of the liaison model are not being fully exploited. By adapting the structures in place to support traditional liaison, such programs can be expanded to include non-academic units as well. As a result, libraries gain opportunities for broader collaboration on campus. Suggestions for identifying targets for formal non-academic liaison are provided, as are guidelines for determining suitable liaison activities and ensuring administrative support within the library.

  14. Library Liaison with non-academic units: a new application for a traditional model

    Directory of Open Access Journals (Sweden)

    Candice Dahl

    2007-03-01

    Full Text Available This paper suggests that traditional models of liaison librarianship, which focuson liaison with academic units such as teaching departments and specificcolleges within institutions, should be adapted and then expanded to include nonacademicunits as well. Although the literature demonstrates that understandingsof liaison work have evolved, it still contains almost no examples of how toextend library liaison beyond traditional academic units in a systematic way. As aresult, existing liaison programs are too narrowly conceived, and the strengths ofthe liaison model are not being fully exploited. By adapting the structures in placeto support traditional liaison, such programs can be expanded to include nonacademicunits as well. As a result, libraries gain opportunities for broadercollaboration on campus. Suggestions for identifying targets for formal nonacademicliaison are provided, as are guidelines for determining suitable liaisonactivities and ensuring administrative support within the library.

  15. Strategic Model for Implementing Knowledge Management in Libraries and Information Services

    Directory of Open Access Journals (Sweden)

    Octavia-Luciana Porumbeanu

    2009-01-01

    Full Text Available In most fields of activity one can notice the trend towards knowledge-based organizations and towards flexible organizations that encourage innovation and change. In this context, knowledge management has become a fundamental process for all types of organizations in society. Libraries and information services are integral part of the knowledge system, these organizations being one of the forms that contribute to knowledge development. This article presents a strategic model for implementing knowledge management in libraries and information services, created on the basis of the results from theoretical researches and practical applications of this process in organizations from different countries and different fields of activity and keeping in mind the specific characteristics of libraries and information services. The model is based on five fundamental elements from which one should begin implementation of a knowledge management function in the organizations engaged in information transfer.

  16. Construction of a large synthetic human Fab antibody library on yeast cell surface by optimized yeast mating.

    Science.gov (United States)

    Baek, Du-San; Kim, Yong-Sung

    2014-03-28

    Yeast surface-displayed antibody libraries provide an efficient and quantitative screening resource for given antigens, but suffer from typically modest library sizes owing to low yeast transformation efficiency. Yeast mating is an attractive method for overcoming the limit of yeast transformation to construct a large, combinatorial antibody library, but the optimal conditions have not been reported. Here, we report a large synthetic human Fab (antigen binding fragment) yeast surface-displayed library generated by stepwise optimization of yeast mating conditions. We first constructed HC (heavy chain) and LC (light chain) libraries, where all of the six CDRs (complementarity-determining regions) of the variable domains were diversified mimicking the human germline antibody repertoires by degenerate codons, onto single frameworks of VH3-23 and Vkappa1-16 germline sequences, in two haploid cells of opposite mating types. Yeast mating conditions were optimized in the order of cell density, media pH, and cell growth phase, yielding a mating efficiency of ~58% between the two haploid cells carrying HC and LC libraries. We constructed two combinatorial Fab libraries with CDR-H3 of 9 or 11 residues in length with colony diversities of more than 10(9) by one round of yeast mating between the two haploid HC and LC libraries, with modest diversity sizes of ~10(7). The synthetic human Fab yeast-displayed libraries exhibited relative amino acid compositions in each position of the six CDRs that were very similar to those of the designed repertoires, suggesting that they are a promising source for human Fab antibody screening.

  17. Virtual Libraries: Interactive Support Software and an Application in Chaotic Models.

    Science.gov (United States)

    Katsirikou, Anthi; Skiadas, Christos; Apostolou, Apostolos; Rompogiannakis, Giannis

    This paper begins with a discussion of the characteristics and the singularity of chaotic systems, including dynamic systems theory, chaotic orbit, fractals, chaotic attractors, and characteristics of chaotic systems. The second section addresses the digital libraries (DL) concept and the appropriateness of chaotic models, including definition and…

  18. mr. A C++ library for the matching and running of the Standard Model parameters

    Energy Technology Data Exchange (ETDEWEB)

    Kniehl, Bernd A.; Veretin, Oleg L. [Hamburg Univ. (Germany). II. Inst. fuer Theoretische Physik; Pikelner, Andrey F. [Hamburg Univ. (Germany). II. Inst. fuer Theoretische Physik; Joint Institute for Nuclear Research, Dubna (Russian Federation). Bogoliubov Lab. of Theoretical Physics

    2016-01-15

    We present the C++ program library mr that allows us to reliably calculate the values of the running parameters in the Standard Model at high energy scales. The initial conditions are obtained by relating the running parameters in the MS renormalization scheme to observables at lower energies with full two-loop precision. The evolution is then performed in accordance with the renormalization group equations with full three-loop precision. Pure QCD corrections to the matching and running are included through four loops. We also provide a Mathematica interface for this program library.

  19. mr: A C++ library for the matching and running of the Standard Model parameters

    Science.gov (United States)

    Kniehl, Bernd A.; Pikelner, Andrey F.; Veretin, Oleg L.

    2016-09-01

    We present the C++ program library mr that allows us to reliably calculate the values of the running parameters in the Standard Model at high energy scales. The initial conditions are obtained by relating the running parameters in the MS bar renormalization scheme to observables at lower energies with full two-loop precision. The evolution is then performed in accordance with the renormalization group equations with full three-loop precision. Pure QCD corrections to the matching and running are included through four loops. We also provide a Mathematica interface for this program library.

  20. mr. A C++ library for the matching and running of the Standard Model parameters

    International Nuclear Information System (INIS)

    We present the C++ program library mr that allows us to reliably calculate the values of the running parameters in the Standard Model at high energy scales. The initial conditions are obtained by relating the running parameters in the MS renormalization scheme to observables at lower energies with full two-loop precision. The evolution is then performed in accordance with the renormalization group equations with full three-loop precision. Pure QCD corrections to the matching and running are included through four loops. We also provide a Mathematica interface for this program library.

  1. mr: a C++ library for the matching and running of the Standard Model parameters

    CERN Document Server

    Kniehl, Bernd A; Veretin, Oleg L

    2016-01-01

    We present the C++ program library mr that allows us to reliably calculate the values of the running parameters in the Standard Model at high energy scales. The initial conditions are obtained by relating the running parameters in the $\\overline{\\mathrm{MS}}$ renormalization scheme to observables at lower energies with full two-loop precision. The evolution is then performed in accordance with the renormalization group equations with full three-loop precision. Pure QCD corrections to the matching and running are included through four loops. We also provide a Mathematica interface for this program library.

  2. Complex Minigene Library Vaccination for Discovery of Pre-Erythrocytic Plasmodium T Cell Antigens

    Science.gov (United States)

    Stone, Brad C.; Kas, Arnold; Billman, Zachary P.; Fuller, Deborah H.; Fuller, James T.; Shendure, Jay; Murphy, Sean C.

    2016-01-01

    Development of a subunit vaccine targeting liver-stage Plasmodium parasites requires the identification of antigens capable of inducing protective T cell responses. However, traditional methods of antigen identification are incapable of evaluating T cell responses against large numbers of proteins expressed by these parasites. This bottleneck has limited development of subunit vaccines against Plasmodium and other complex intracellular pathogens. To address this bottleneck, we are developing a synthetic minigene technology for multi-antigen DNA vaccines. In an initial test of this approach, pools of long (150 bp) antigen-encoding oligonucleotides were synthesized and recombined into vectors by ligation-independent cloning to produce two DNA minigene library vaccines. Each vaccine encoded peptides derived from 36 (vaccine 1) and 53 (vaccine 2) secreted or transmembrane pre-erythrocytic P. yoelii proteins. BALB/cj mice were vaccinated three times with a single vaccine by biolistic particle delivery (gene gun) and screened for interferon-γ-producing T cell responses by ELISPOT. Library vaccination induced responses against four novel antigens. Naïve mice exposed to radiation-attenuated sporozoites mounted a response against only one of the four novel targets (PyMDH, malate dehydrogenase). The response to PyMDH could not be recalled by additional homologous sporozoite immunizations but could be partially recalled by heterologous cross-species sporozoite exposure. Vaccination against the dominant PyMDH epitope by DNA priming and recombinant Listeria boosting did not protect against sporozoite challenge. Improvements in library design and delivery, combined with methods promoting an increase in screening sensitivity, may enable complex minigene screening to serve as a high-throughput system for discovery of novel T cell antigens. PMID:27070430

  3. Identification of new hematopoietic cell subsets with a polyclonal antibody library specific for neglected proteins.

    Directory of Open Access Journals (Sweden)

    Monica Moro

    Full Text Available The identification of new markers, the expression of which defines new phenotipically and functionally distinct cell subsets, is a main objective in cell biology. We have addressed the issue of identifying new cell specific markers with a reverse proteomic approach whereby approximately 1700 human open reading frames encoding proteins predicted to be transmembrane or secreted have been selected in silico for being poorly known, cloned and expressed in bacteria. These proteins have been purified and used to immunize mice with the aim of obtaining polyclonal antisera mostly specific for linear epitopes. Such a library, made of about 1600 different polyclonal antisera, has been obtained and screened by flow cytometry on cord blood derived CD34+CD45dim cells and on peripheral blood derived mature lymphocytes (PBLs. We identified three new proteins expressed by fractions of CD34+CD45dim cells and eight new proteins expressed by fractions of PBLs. Remarkably, we identified proteins the presence of which had not been demonstrated previously by transcriptomic analysis. From the functional point of view, looking at new proteins expressed on CD34+CD45dim cells, we identified one cell surface protein (MOSC-1 the expression of which on a minority of CD34+ progenitors marks those CD34+CD45dim cells that will go toward monocyte/granulocyte differentiation. In conclusion, we show a new way of looking at the membranome by assessing expression of generally neglected proteins with a library of polyclonal antisera, and in so doing we have identified new potential subsets of hematopoietic progenitors and of mature PBLs.

  4. Investigation of the Mesenchymal Stem Cell Compartment by Means of a Lentiviral Barcode Library.

    Science.gov (United States)

    Bigildeev, A E; Cornils, K; Aranyossy, T; Sats, N V; Petinati, N A; Shipounova, I N; Surin, V L; Pshenichnikova, O S; Riecken, K; Fehse, B; Drize, N I

    2016-04-01

    The hematopoietic bone marrow microenvironment is formed by proliferation and differentiation of mesenchymal stem cells (MSCs). The MSC compartment has been less studied than the hematopoietic stem cell compartment. To characterize the structure of the MSC compartment, it is necessary to trace the fate of distinct mesenchymal cells. To do so, mesenchymal progenitors need to be marked at the single-cell level. A method for individual marking of normal and cancer stem cells based on genetic "barcodes" has been developed for the last 10 years. Such approach has not yet been applied to MSCs. The aim of this study was to evaluate the possibility of using such barcoding strategy to mark MSCs and their descendants, colony-forming units of fibroblasts (CFU-Fs). Adherent cell layers (ACLs) of murine long-term bone marrow cultures (LTBMCs) were transduced with a lentiviral library with barcodes consisting of 32 + 3 degenerate nucleotides. Infected ACLs were suspended, and CFU-F derived clones were obtained. DNA was isolated from each individual colony, and barcodes were analyzed in marked CFU-F-derived colonies by means of conventional polymerase chain reaction and Sanger sequencing. Barcodes were identified in 154 marked colonies. All barcodes appeared to be unique: there were no two distinct colonies bearing the same barcode. It was shown that ACLs included CFU-Fs with different proliferative potential. MSCs are located higher in the hierarchy of mesenchymal progenitors than CFU-Fs, so the presented data indicate that MSCs proliferate rarely in LTBMCs. A method of stable individual marking and comparing the markers in mesenchymal progenitor cells has been developed in this work. We show for the first time that a barcoded library of lentiviruses is an effective tool for studying stromal progenitor cells. PMID:27293094

  5. Hydrologic Nuclide Transport Models in Cyder, A Geologic Disposal Software Library - 13328

    Energy Technology Data Exchange (ETDEWEB)

    Huff, Kathryn D. [Argonne National Laboratory, 9700 S. Cass Ave, Argonne, IL (United States)

    2013-07-01

    Component level and system level abstraction of detailed computational geologic repository models have resulted in four rapid computational models of hydrologic radionuclide transport at varying levels of detail. Those models are described, as is their implementation in Cyder, a software library of interchangeable radionuclide transport models appropriate for representing natural and engineered barrier components of generic geology repository concepts. A proof of principle demonstration was also conducted in which these models were used to represent the natural and engineered barrier components of a repository concept in a reducing, homogenous, generic geology. This base case demonstrates integration of the Cyder open source library with the Cyclus computational fuel cycle systems analysis platform to facilitate calculation of repository performance metrics with respect to fuel cycle choices. (authors)

  6. REAL-Select: full-length antibody display and library screening by surface capture on yeast cells.

    Directory of Open Access Journals (Sweden)

    Laura Rhiel

    Full Text Available We describe a novel approach named REAL-Select for the non-covalent display of IgG-molecules on the surface of yeast cells for the purpose of antibody engineering and selection. It relies on the capture of secreted native full-length antibodies on the cell surface via binding to an externally immobilized ZZ domain, which tightly binds antibody Fc. It is beneficial for high-throughput screening of yeast-displayed IgG-libraries during antibody discovery and development. In a model experiment, antibody-displaying yeast cells were isolated from a 1:1,000,000 mixture with control cells confirming the maintenance of genotype-phenotype linkage. Antibodies with improved binding characteristics were obtained by affinity maturation using REAL-Select, demonstrating the ability of this system to display antibodies in their native form and to detect subtle changes in affinity by flow cytometry. The biotinylation of the cell surface followed by functionalization with a streptavidin-ZZ fusion protein is an approach that is independent of the genetic background of the antibody-producing host and therefore can be expected to be compatible with other eukaryotic expression hosts such as P. pastoris or mammalian cells.

  7. A Critical Information Literacy Model: Library Leadership within the Curriculum

    Science.gov (United States)

    Swanson, Troy

    2011-01-01

    It is a time for a new model for teaching students to find, evaluate, and use information by drawing on critical pedagogy theory in the education literature. This critical information literacy model views the information world as a dynamic place where authors create knowledge for many reasons; it seeks to understand students as information users,…

  8. Conceptual models in the field of library catalogues

    Directory of Open Access Journals (Sweden)

    Marija Petek

    2000-01-01

    Full Text Available The publishing world is changing quickly and so must also bibliographic control. It is tirne to re-examine cataloguing rules and MARC formats. This can be done by the method of conceptual modelling. Some conceptual models are presented; an IFLA study on the functional requirements for bibliographic records is described in detail.

  9. Geographic location as an access model to library collections – a pilot project of European Digital Library

    Directory of Open Access Journals (Sweden)

    Dalibor Radovan

    2007-01-01

    Full Text Available In the frames of the European eContentplus project European Digital Library (EDL a pilot GIS application was integrated in The European Library (TEL portal. The application was realized in the cooperation between the National and University Library of Slovenia and the Geodetic Institute of Slovenia. The pilot GIS application represents the beginning of the development of digital geolibrary where a map is the starting point leading to different geodifferentiated resources. At the same time, it also represents an upgrade of the static presentation of different kinds of digitised materials in the library, as well as an attempt of interdisciplinary presentation with additional educational value. The goal, content, methodology and the results of the pilot GIS application are presented in the article.

  10. Construction of a Sequencing Library from Circulating Cell-Free DNA.

    Science.gov (United States)

    Fang, Nan; Löffert, Dirk; Akinci-Tolun, Rumeysa; Heitz, Katja; Wolf, Alexander

    2016-01-01

    Circulating DNA is cell-free DNA (cfDNA) in serum or plasma that can be used for non-invasive prenatal testing, as well as cancer diagnosis, prognosis, and stratification. High-throughput sequence analysis of the cfDNA with next-generation sequencing technologies has proven to be a highly sensitive and specific method in detecting and characterizing mutations in cancer and other diseases, as well as aneuploidy during pregnancy. This unit describes detailed procedures to extract circulating cfDNA from human serum and plasma and generate sequencing libraries from a wide concentration range of circulating DNA. © 2016 by John Wiley & Sons, Inc. PMID:27038390

  11. Leishmania chagasi T-cell antigens identified through a double library screen.

    Science.gov (United States)

    Martins, Daniella R A; Jeronimo, Selma M B; Donelson, John E; Wilson, Mary E

    2006-12-01

    Control of human visceral leishmaniasis in regions where it is endemic is hampered in part by limited accessibility to medical care and emerging drug resistance. There is no available protective vaccine. Leishmania spp. protozoa express multiple antigens recognized by the vertebrate immune system. Since there is not one immunodominant epitope recognized by most hosts, strategies must be developed to optimize selection of antigens for prevention and immunodiagnosis. For this reason, we generated a cDNA library from the intracellular amastigote form of Leishmania chagasi, the cause of South American visceral leishmaniasis. We employed a two-step expression screen of the library to systematically identify T-cell antigens and T-dependent B-cell antigens. The first step was aimed at identifying the largest possible number of clones producing an epitope-containing polypeptide by screening with a pool of sera from Brazilians with documented visceral leishmaniasis. After removal of clones encoding heat shock proteins, positive clones underwent a second-step screen for their ability to cause proliferation and gamma interferon responses in T cells from immune mice. Six unique clones were selected from the second screen for further analysis. The corresponding antigens were derived from glutamine synthetase, a transitional endoplasmic reticulum ATPase, elongation factor 1gamma, kinesin K39, repetitive protein A2, and a hypothetical conserved protein. Humans naturally infected with L. chagasi mounted both cellular and antibody responses to these proteins. Preparations containing multiple antigens may be optimal for immunodiagnosis and protective vaccines. PMID:17000724

  12. Rapid isolation of antibody from a synthetic human antibody library by repeated fluorescence-activated cell sorting (FACS.

    Directory of Open Access Journals (Sweden)

    Sung Sun Yim

    Full Text Available Antibodies and their derivatives are the most important agents in therapeutics and diagnostics. Even after the significant progress in the technology for antibody screening from huge libraries, it takes a long time to isolate an antibody, which prevents a prompt action against the spread of a disease. Here, we report a new strategy for isolating desired antibodies from a combinatorial library in one day by repeated fluorescence-activated cell sorting (FACS. First, we constructed a library of synthetic human antibody in which single-chain variable fragment (scFv was expressed in the periplasm of Escherichia coli. After labeling the cells with fluorescent antigen probes, the highly fluorescent cells were sorted by using a high-speed cell sorter, and these cells were reused without regeneration in the next round of sorting. After repeating this sorting, the positive clones were completely enriched in several hours. Thus, we screened the library against three viral antigens, including the H1N1 influenza virus, Hepatitis B virus, and Foot-and-mouth disease virus. Finally, the potential antibody candidates, which show K(D values between 10 and 100 nM against the target antigens, could be successfully isolated even though the library was relatively small (∼ 10(6. These results show that repeated FACS screening without regeneration of the sorted cells can be a powerful method when a rapid response to a spreading disease is required.

  13. Generation and selection of immunized Fab phage display library against human B cell lymphoma

    Institute of Scientific and Technical Information of China (English)

    Yongmei Shen; Xiaochun Yang; Ningzheng Dong; Xiaofang Xie; Xia Bai; Yizhen Shi

    2007-01-01

    The approval of using monoclonal antibodies as a targeted therapy in the management of patients with B cell lymphoma has led to new treatment options for this group of patients. Production of monoclonal antibodies by the traditional hybridoma technology is costly, and the resulting murine antibodies often have the disadvantage of triggering human anti-mouse antibody (HAMA) response. Therefore recombinant Fab antibodies generated by the phage display technology can be a suitable alternative in managing B cell lymphoma. In this study, we extracted total RNA from spleen cells of BALB/c mice immunized with human B lymphoma cells, and used RT-PCR to amplify cDNAs coding for the K light chains and Fd fragments of heavy chains. After appropriate restriction digests, these cDNA fragments were successively inserted into the phagemid vector pComb3H-SS to construct an immunized Fab phage display library. The diversity of the constructed library was approximately 1.94×107. Following five rounds of biopanning, soluble Fab antibodies were produced from positive clones identified by ELISA. From eight positive clones, FabC06, FabC21, FabC43 and FabC59 were selected for sequence analysis. At the level of amino acid sequences, the variable heavy domains (VH) and variable light domains (VL) were found to share 88-92% and 89-94% homology with sequences coded by the corresponding murine germline genes respectively. Furthermore, reactivity with membrane proteins of the B cell lymphoma was demonstrated by immunohistochemistry and western blotting. These immunized Fab antibodies may provide a valuable tool for further study of B cell lymphoma and could also contribute to the improvement of disease therapy.

  14. Client value models provide a framework for rational library planning (or, phrasing the answer in the form of a question).

    Science.gov (United States)

    Van Moorsel, Guillaume

    2005-01-01

    Libraries often do not know how clients value their product/ service offerings. Yet at a time when the mounting costs for library support are increasingly difficult to justify to the parent institution, the library's ability to gauge the value of its offerings to clients has never been more critical. Client Value Models (CVMs) establish a common definition of value elements-or a "value vocabulary"-for libraries and their clients, thereby providing a basis upon which to make rational planning decisions regarding product/service acquisition and development. The CVM concept is borrowed from business and industry, but its application has a natural fit in libraries. This article offers a theoretical consideration and practical illustration of CVM application in libraries.

  15. PeptideBuilder: A simple Python library to generate model peptides

    Directory of Open Access Journals (Sweden)

    Matthew Z. Tien

    2013-05-01

    Full Text Available We present a simple Python library to construct models of polypeptides from scratch. The intended use case is the generation of peptide models with pre-specified backbone angles. For example, using our library, one can generate a model of a set of amino acids in a specific conformation using just a few lines of python code. We do not provide any tools for energy minimization or rotamer packing, since powerful tools are available for these purposes. Instead, we provide a simple Python interface that enables one to add residues to a peptide chain in any desired conformation. Bond angles and bond lengths can be manipulated if so desired, and reasonable values are used by default.

  16. Effects of the EVCAM chemical validation library on differentiation using marker gene expression in lmouse embryonic stem cells

    Science.gov (United States)

    The adherent cell differentiation and cytotoxicity (ACDC) assay was used to profile the effects of the ECVAM EST validation chemical library (19 compounds) on J1 mouse embryonic stem cells (mESC). PCR-based TaqMan Low Density Arrays (TLDA) provided a high-content assessment of al...

  17. Construction and analysis of SSH cDNA library of human vascular endothelial cells related to gastrocarcinoma

    Institute of Scientific and Technical Information of China (English)

    Yong-Bo Liu; Zhao-Xia Wei; Li Li; Hang-Sheng Li; Hui Chen; Xiao-Wen Li

    2003-01-01

    AIM: To construct subtracted cDNA libraries of human vascular endothelial cells (VECs) related to gastrocarcinoma using suppression substractive hybridization (SSH) and to analyze cDNA libraries of gastrocarcinoma and VECs in Cancer Gene Anatomy Project (CGAP) database.METHODS: Human VECs related to gastric adenocarcinoma and corresponding normal tissue were separated by magnetic beads coupled with antibody CD31 (Dynabeads CD31). A few amount of total RNA were synthesized and amplified by SMARTTM PCR cDNA Synthesis Kit. Then, using SSH and T/A cloning techniques, cDNA fragments of differentially expressed genes in human VECs of gastric adenocarcinoma were inserted into JM109 bacteria. One hundred positive bacteria clones were randomly picked and identified by colony PCR method. To analyze cDNA libraries of gastrocarcinoma and VECs in CGAP database, the tools of Library Finder,cDNA xProfiler, Digital GENE Expression Displayer (DGED),and Digital Differential Display (DDD) were used.RESULTS: Forward and reverse subtraction cDNA libraries of human VECs related to gastrocarcinoma were constructed successfully with SSH and T/A cloning techniques. Analysis of CGAP database indicated that no appropriate library of VECs related to carcinoma was constructed.CONCLUSION: Construction of subtraction cDNA libraries of human VECs related to gastrocarcinoma was successful and necessary, which laid a foundation for screening and cloning new and specific genes of VECs related to gastrocardnoma.

  18. Characterization of Leukemia-Inducing Genes Using a Proto-Oncogene/Homeobox Gene Retroviral Human cDNA Library in a Mouse In Vivo Model.

    Science.gov (United States)

    Jang, Su Hwa; Lee, Sohyun; Chung, Hee Yong

    2015-01-01

    The purpose of this research is to develop a method to screen a large number of potential driver mutations of acute myeloid leukemia (AML) using a retroviral cDNA library and murine bone marrow transduction-transplantation system. As a proof-of-concept, murine bone marrow (BM) cells were transduced with a retroviral cDNA library encoding well-characterized oncogenes and homeobox genes, and the virus-transduced cells were transplanted into lethally irradiated mice. The proto-oncogenes responsible for leukemia initiation were identified by PCR amplification of cDNA inserts from genomic DNA isolated from leukemic cells. In an initial screen of ten leukemic mice, the MYC proto-oncogene was detected in all the leukemic mice. Of ten leukemic mice, 3 (30%) had MYC as the only transgene, and seven mice (70%) had additional proto-oncogene inserts. We repeated the same experiment after removing MYC-related genes from the library to characterize additional leukemia-inducing gene combinations. Our second screen using the MYC-deleted proto-oncogene library confirmed MEIS1and the HOX family as cooperating oncogenes in leukemia pathogenesis. The model system we introduced in this study will be valuable in functionally screening novel combinations of genes for leukemogenic potential in vivo, and the system will help in the discovery of new targets for leukemia therapy.

  19. Characterization of Leukemia-Inducing Genes Using a Proto-Oncogene/Homeobox Gene Retroviral Human cDNA Library in a Mouse In Vivo Model.

    Directory of Open Access Journals (Sweden)

    Su Hwa Jang

    Full Text Available The purpose of this research is to develop a method to screen a large number of potential driver mutations of acute myeloid leukemia (AML using a retroviral cDNA library and murine bone marrow transduction-transplantation system. As a proof-of-concept, murine bone marrow (BM cells were transduced with a retroviral cDNA library encoding well-characterized oncogenes and homeobox genes, and the virus-transduced cells were transplanted into lethally irradiated mice. The proto-oncogenes responsible for leukemia initiation were identified by PCR amplification of cDNA inserts from genomic DNA isolated from leukemic cells. In an initial screen of ten leukemic mice, the MYC proto-oncogene was detected in all the leukemic mice. Of ten leukemic mice, 3 (30% had MYC as the only transgene, and seven mice (70% had additional proto-oncogene inserts. We repeated the same experiment after removing MYC-related genes from the library to characterize additional leukemia-inducing gene combinations. Our second screen using the MYC-deleted proto-oncogene library confirmed MEIS1and the HOX family as cooperating oncogenes in leukemia pathogenesis. The model system we introduced in this study will be valuable in functionally screening novel combinations of genes for leukemogenic potential in vivo, and the system will help in the discovery of new targets for leukemia therapy.

  20. Library Liaison with non-academic units: a new application for a traditional model

    OpenAIRE

    Candice Dahl

    2007-01-01

    This paper suggests that traditional models of liaison librarianship, which focus on liaison with academic units such as teaching departments and specific colleges within institutions, should be adapted and then expanded to include non-academic units as well. Although the literature demonstrates that understandings of liaison work have evolved, it still contains almost no examples of how to extend library liaison beyond traditional academic units in a systematic way. As a result, existing l...

  1. Nonlinear model reduction for dynamical systems using sparse sensor locations from learned libraries

    Science.gov (United States)

    Sargsyan, Syuzanna; Brunton, Steven L.; Kutz, J. Nathan

    2015-09-01

    We demonstrate the synthesis of sparse sampling and dimensionality reduction to characterize and model nonlinear dynamical systems over a range of bifurcation parameters. First, we construct modal libraries using the classical proper orthogonal decomposition in order to expose the dominant low-rank coherent structures. Here, libraries of the nonlinear terms are also constructed in order to take advantage of the discrete empirical interpolation method and projection that allows for the approximation of nonlinear terms from a sparse number of grid points. The selected grid points are shown to be effective sensing and measurement locations for characterizing the underlying dynamics, stability, and bifurcations of nonlinear dynamical systems. The use of empirical interpolation points and sparse representation facilitates a family of local reduced-order models for each physical regime, rather than a higher-order global model, which has the benefit of physical interpretability of energy transfer between coherent structures. The method advocated also allows for orders-of-magnitude improvement in computational speed and memory requirements. To illustrate the method, the discrete interpolation points and nonlinear modal libraries are used for sparse representation in order to classify and reconstruct the dynamic bifurcation regimes in the complex Ginzburg-Landau equation. It is also shown that point measurements of the nonlinearity are more effective than linear measurements when sensor noise is present.

  2. Differentiated digital library evaluation in a hierarchical model stemming from its operational scope and complexity

    Institute of Scientific and Technical Information of China (English)

    WU; Jianhua; WANG; Zhaohui

    2009-01-01

    Digital libraries are complex systems and this brings difficulties for their evaluation.This paper proposes a hierarchical model to solve this problem,and puts the entangled matters into a clear-layered structure.Firstly,digital libraries(DLs thereafter)are classified into 5 groups in ascending gradations,i.e.mini DLs,small DLs,medium DLs,large DLs,and huge DLs by their scope of operation.Then,according to the characteristics of DLs at different operational scope and level of sophistication,they are further grouped into unitary DLs,union DLs and hybrid DLs accordingly.Based on this simulated structure,a hierarchical model for digital library evaluation is introduced,which evaluates DLs differentiatingly within a hierarchical scheme by using varying criteria based on their specific level of operational complexity such as at the micro-level,medium-level,and/or at the macro-level.Based on our careful examination and analysis of the current literature about DL evaluation system,an experiment is conducted by using the DL evaluation model along with its criteria for unitary DLs at micro-level.The main contents resulting from this evaluation experimentation and also those evaluation indicators and relevant issues of major concerns for DLs at medium-level and macro-level are also to be presented at some length.

  3. RSL: A parallel Runtime System Library for regional atmospheric models with nesting

    Energy Technology Data Exchange (ETDEWEB)

    Michalakes, J.G.

    1997-08-01

    RSL is a parallel runtime system library developed at Argonne National Laboratory that is tailored to regular-grid atmospheric models with mesh refinement in the form of two-way interacting nested grids. RSL provides high-level stencil and interdomain communication, irregular domain decomposition, automatic local/global index translation, distributed I/O, and dynamic load balancing. RSL was used with Fortran90 to parallelize a well-known and widely used regional weather model, the Penn State/NCAR Mesoscale model.

  4. Development of 3D Female Breast Model Library for Bra Design

    Institute of Scientific and Technical Information of China (English)

    WANG Jian-ping; ZHANG Wei-yuan

    2006-01-01

    As the maturity of female costume concept as well as the social consuming activities, a new and higher requirement is imposed on female underwear design. Human body model is the basic of many applications on 3D garment CAD. This paper delivered a novel approach for modeling a human body which could be driven by related body dimensions to form a female breast model library based on the free form deformation technologies. The 3D female breast shape reserve has a strong potential of being used for bra design,bra fitting, virtual try-on and exhibition to meet "made-tomeasure" demand of the booming bra market in the world.

  5. The Scandinavian Library of Athens is giving a model for Greek culture

    OpenAIRE

    Kotka, Mari

    2003-01-01

    The Scandinavian Library of Athens concentrates on literature on Greek archaeology, on ancient Greek history, religion, art and history of culture. Since the library use in Greece is not as common as in Scandinavia, the Library provides a good example of the possibilities of a modern archaeological library. The Library is a reference library. It uses UNIMARC based ABEKT program for cataloguing. It participates in the ARGOS project, which aims to combine in one database all the collections of ...

  6. Methods for transforming and expression screening of filamentous fungal cells with a DNA library

    Energy Technology Data Exchange (ETDEWEB)

    Teter, Sarah; Lamsa, Michael; Cherry, Joel; Ward, Connie

    2015-06-02

    The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides. The present invention also relates to isolated polynucleotides encoding polypeptides of interest obtained by such methods, to nucleic acid constructs, expression vectors, and recombinant host cells comprising the isolated polynucleotides, and to methods of producing the polypeptides encoded by the isolated polynucleotides.

  7. ALL-Digital Baseband 65nm PLL/FPLL Clock Multiplier Using 10-Cell Library

    Science.gov (United States)

    Schuler, Robert L., Jr.; Wu, Qiong; Liu, Rui; Chen, Li; Madala, Shridhar

    2014-01-01

    PLLs for clock generation are essential for modern circuits, to generate specialized frequencies for many interfaces and high frequencies for chip internal operation. These circuits depend on analog circuits and careful tailoring for each new process, and making them fault tolerant is an incompletely solved problem. Until now, all digital PLLs have been restricted to sampled data DSP techniques and not available for the highest frequency baseband applications. This paper presents the design and preliminary evaluation of an all-digital baseband technique built entirely with an easily portable 10-cell digital library. The library is also described, as it aids in research and low volume design porting to new processes. The advantages of the digital approach are the wide variety of techniques available to give varying degrees of fault tolerance, and the simplicity of porting the design to new processes, even to exotic processes that may not have analog capability. The only tuning parameter is digital gate delay. An all-digital approach presents unique problems and standard analog loop stability design criteria cannot be directly used. Because of the quantization of frequency, there is effectively infinite gain for very small loop error feedback. The numerically controlled oscillator (NCO) based on a tapped delay line cannot be reliably updated while a pulse is active in the delay line, and ordinarily does not have enough frequency resolution for a low-jitter output.

  8. Diversity oriented fluorescence library approach (DOFLA) for live cell imaging probe development.

    Science.gov (United States)

    Yun, Seong-Wook; Kang, Nam-Young; Park, Sung-Jin; Ha, Hyung-Ho; Kim, Yun Kyung; Lee, Jun-Seok; Chang, Young-Tae

    2014-04-15

    A cell is the smallest functional unit of life. All forms of life rely on cellular processes to maintain normal functions, and changes in cell function induced by metabolic disturbances, physicochemical damage, infection, or abnormal gene expression may cause disease. To understand basic biology and to develop therapeutics for diseases, researchers need to study live cells. Along with advances in fluorescence microscopy and in vitro cell culture, live-cell imaging has become an essential tool in modern biology for the study of molecular and cellular events. Although researchers have often used fluorescent proteins to visualize cell-type-specific markers, this method requires genetic manipulations, which may not be appropriate in nontransgenic cells. Immunodetection of cellular markers requires the use of xenogenic antibodies, which may not detect intracellular markers in live cells. One option for overcoming these problems is the use of fluorescent small molecules targeted to specific cell types, which can enter live cells and interact with molecules of interest. We have used combinatorial chemistry to develop a large number of fluorescent small molecules as new imaging probes even without prior information about the probes' binding targets and mechanism, a strategy that we call the diversity oriented fluorescence library approach (DOFLA). We have used DOFLA to produce novel sensors and probes that detect a variety of biological and chemical molecules in vivo as well as in vitro. In this Account, we describe a series of fluorescent small molecules developed using DOFLA that bind specifically to particular cell types. These molecules provide new ways to detect and isolate these cells. The fluorescent probes CDy1, CDg4, and CDb8 tag embryonic stem cells and induced pluripotent stem cells but not fibroblasts or germ-line cells. CDr3 binds to an intracellular neural stem cell marker, fatty acid binding protein 7, which allows researchers to separate neural stem cells

  9. Identifying differential expression in multiple SAGE libraries: an overdispersed log-linear model approach

    Directory of Open Access Journals (Sweden)

    Kepler Thomas B

    2005-06-01

    Full Text Available Abstract Background In testing for differential gene expression involving multiple serial analysis of gene expression (SAGE libraries, it is critical to account for both between and within library variation. Several methods have been proposed, including the t test, tw test, and an overdispersed logistic regression approach. The merits of these tests, however, have not been fully evaluated. Questions still remain on whether further improvements can be made. Results In this article, we introduce an overdispersed log-linear model approach to analyzing SAGE; we evaluate and compare its performance with three other tests: the two-sample t test, tw test and another based on overdispersed logistic linear regression. Analysis of simulated and real datasets show that both the log-linear and logistic overdispersion methods generally perform better than the t and tw tests; the log-linear method is further found to have better performance than the logistic method, showing equal or higher statistical power over a range of parameter values and with different data distributions. Conclusion Overdispersed log-linear models provide an attractive and reliable framework for analyzing SAGE experiments involving multiple libraries. For convenience, the implementation of this method is available through a user-friendly web-interface available at http://www.cbcb.duke.edu/sage.

  10. New software library of geometrical primitives for modelling of solids used in Monte Carlo detector simulations

    CERN Document Server

    CERN. Geneva

    2012-01-01

    We present our effort for the creation of a new software library of geometrical primitives, which are used for solid modelling in Monte Carlo detector simulations. We plan to replace and unify current geometrical primitive classes in the CERN software projects Geant4 and ROOT with this library. Each solid is represented by a C++ class with methods suited for measuring distances of particles from the surface of a solid and for determination as to whether the particles are located inside, outside or on the surface of the solid. We use numerical tolerance for determining whether the particles are located on the surface. The class methods also contain basic support for visualization. We use dedicated test suites for validation of the shape codes. These include also special performance and numerical value comparison tests for help with analysis of possible candidates of class methods as well as to verify that our new implementation proposals were designed and implemented properly. Currently, bridge classes are u...

  11. Selection of functional T cell receptor mutants from a yeast surface-display library.

    Science.gov (United States)

    Kieke, M C; Shusta, E V; Boder, E T; Teyton, L; Wittrup, K D; Kranz, D M

    1999-05-11

    The heterodimeric alphabeta T cell receptor (TCR) for antigen is the key determinant of T cell specificity. The structure of the TCR is very similar to that of antibodies, but the engineering of TCRs by directed evolution with combinatorial display libraries has not been accomplished to date. Here, we report that yeast surface display of a TCR was achieved only after the mutation of specific variable region residues. These residues are located in two regions of the TCR, at the interface of the alpha- and beta-chains and in the beta-chain framework region that is thought to be in proximity to the CD3 signal-transduction complex. The mutations are encoded naturally in many antibody variable regions, indicating specific functional differences that have not been appreciated between TCRs and antibodies. The identification of these residues provides an explanation for the inherent difficulties in the display of wild-type TCRs compared with antibodies. Yeast-displayed mutant TCRs bind specifically to the peptide/MHC antigen, enabling engineering of soluble T cell receptors as specific T cell antagonists. This strategy of random mutagenesis followed by selection for surface expression may be of general use in the directed evolution of other eukaryotic proteins that are refractory to display.

  12. Characterizing and Modeling the Cost of Rework in a Library of Reusable Software Components

    Science.gov (United States)

    Basili, Victor R.; Condon, Steven E.; ElEmam, Khaled; Hendrick, Robert B.; Melo, Walcelio

    1997-01-01

    In this paper we characterize and model the cost of rework in a Component Factory (CF) organization. A CF is responsible for developing and packaging reusable software components. Data was collected on corrective maintenance activities for the Generalized Support Software reuse asset library located at the Flight Dynamics Division of NASA's GSFC. We then constructed a predictive model of the cost of rework using the C4.5 system for generating a logical classification model. The predictor variables for the model are measures of internal software product attributes. The model demonstrates good prediction accuracy, and can be used by managers to allocate resources for corrective maintenance activities. Furthermore, we used the model to generate proscriptive coding guidelines to improve programming, practices so that the cost of rework can be reduced in the future. The general approach we have used is applicable to other environments.

  13. New software library of geometrical primitives for modeling of solids used in Monte Carlo detector simulations

    International Nuclear Information System (INIS)

    We present our effort for the creation of a new software library of geometrical primitives, which are used for solid modeling in Monte Carlo detector simulations. We plan to replace and unify the current implementations for geometrical primitive classes in the software projects Geant4 and ROOT with this library. Each solid is implemented as a C++ class providing methods to compute distances of rays to the surface of a solid or to find whether a position is located inside, outside or on the surface of the solid. A numerical tolerance is used for determining whether a position is on the surface. The class methods also contain basic support for visualization. We use dedicated test suites for the validation of the code; these also include performance and consistency tests used for the analysis of candidate implementations of class methods for the new library. We have implemented simple adapter classes to allow the use of the new optimized solids with Geant4 and ROOT geometries.

  14. SOSpin, a C++ library for Yukawa decomposition in SO(2 N) models

    Science.gov (United States)

    Cardoso, Nuno; Emmanuel-Costa, David; Gonçalves, Nuno; Simões, C.

    2016-06-01

    We present in this paper the SOSpin library, which calculates an analytic decomposition of the Yukawa interactions invariant under SO(2N) in terms of an SU(N) basis. We make use of the oscillator expansion formalism, where the SO(2N) spinor representations are expressed in terms of creation and annihilation operators of a Grassmann algebra acting on a vacuum state. These noncommutative operators and their products are simulated in SOSpin through the implementation of doubly-linked-list data structures. These data structures were determinant to achieve a higher performance in the simplification of large products of creation and annihilation operators. We illustrate the use of our library with complete examples of how to decompose Yukawa terms invariant under SO(2N) in terms of SU(N) degrees of freedom for N=2N=2 and 5. We further demonstrate, with an example for SO(4), that higher dimensional field-operator terms can also be processed with our library. Finally, we describe the functions available in SOSpin that are made to simplify the writing of spinors and their interactions specifically for SO(10) models.

  15.   Cell Penetrating Peptoids (CPPos: Synthesis of a Small Combinatorial Library by Using IRORI MiniKans

    Directory of Open Access Journals (Sweden)

    Dominik K. Kölmel

    2012-11-01

    Full Text Available Cell penetrating peptoids (CPPos are potent mimics of the corresponding cell penetrating peptides (CPPs. The synthesis of diverse oligomeric libraries that display a variety of backbone scaffolds and side-chain appendages are a very promising source of novel CPPos, which can be used to either target different cellular organelles or even different tissues and organs. In this study we established the submonomer-based solid phase synthesis of a “proof of principle” peptoid library in IRORI MiniKans to expand the amount for phenotypic high throughput screens of CPPos. The library consisting of tetrameric peptoids [oligo(N-alkylglycines] was established on Rink amide resin in a split and mix approach with hydrophilic and hydrophobic peptoid side chains. All CPPos of the presented library were labeled with rhodamine B to allow for the monitoring of cellular uptake by fluorescent confocal microscopy. Eventually, all the purified peptoids were subjected to live cell imaging to screen for CPPos with organelle specificity. While highly charged CPPos enter the cells by endocytosis with subsequent endosomal release, critical levels of lipophilicity allow other CPPos to specifically localize to mitochondria once a certain lipophilicity threshold is reached.

  16. A Study of compatibility between Iranian digital libraries user interface and the elements of Ellis model of information seeking behavior

    Directory of Open Access Journals (Sweden)

    Yaghoub Norouzi

    2014-10-01

    Full Text Available The goal of this study is to evaluate the compatibility between Iranian digital libraries user interface and the elements of Ellis model of information seeking behavior. This study was performed using evaluation method with a checklist. This list is based on six features of Ellis model, "Starting", "Chaining", "Browsing", "Differentiating", "Monitoring" and "Extracting" and included 110 sub-components that have been developed using Delphi method. The population of this study included 5 library softwares: Azarakhsh, Simorgh, Papyrus, Payam and Parvan. Results showed that the average of scores of user interface of softwares is as follows: in Starting 99.6, Chaining 117.2, Browsing 29.6, Differentiating 53. 2, Monitoring 22.4 and Extracting 39. Among the softwares of digital libraries, according to the total score for elements of Ellis model (1080, Azarakhsh with 549 points is in the first rank and had the highest support for elements of Ellis model. It also became clear that Chaining with 586 points (%55 had the highest support in softwares of digital libraries. Also the elements of Chaining, Starting and Differentiating earned sequently the highest rank in softwares of digital libraries. Finally, testing research hypotheses with %95 confidence showed that among softwares of digital libraries there is no significant difference regarding the rate of support of elements of information seeking behavior of Ellis model.

  17. Reforming Prison Libraries.

    Science.gov (United States)

    Coyle, William J.

    1989-01-01

    Discusses the current widespread acceptance of the public library model for prison libraries, in which preferences of the inmates are the chief consideration in programing and collection development. It is argued that this model results in recreational programs and collections that fail to fulfill the prison library's role in education and…

  18. Single-stranded DNA library preparation uncovers the origin and diversity of ultrashort cell-free DNA in plasma

    OpenAIRE

    Philip Burnham; Min Seong Kim; Sean Agbor-Enoh; Helen Luikart; Hannah A. Valantine; Kiran K Khush; Iwijn De Vlaminck

    2016-01-01

    Circulating cell-free DNA (cfDNA) is emerging as a powerful monitoring tool in cancer, pregnancy and organ transplantation. Nucleosomal DNA, the predominant form of plasma cfDNA, can be adapted for sequencing via ligation of double-stranded DNA (dsDNA) adapters. dsDNA library preparations, however, are insensitive to ultrashort, degraded cfDNA. Drawing inspiration from advances in paleogenomics, we have applied a single-stranded DNA (ssDNA) library preparation method to sequencing of cfDNA in...

  19. Structural analysis and molecular modeling of two antitrichosanthin IgE clones from phage antibody library

    Institute of Scientific and Technical Information of China (English)

    LIZONGDONG; YURENYUAN; 等

    1997-01-01

    Recently we constructed a murine IgE phage surface display library and screened out two IgE (Fab) clones with specific binding activity to Trichosanthin (TCS).In this work,the Vε and Vκ genes of the two clones were sequenced and their putative germline gene usages were studied.On the basis of the known 3D structure of Trichosanthin and antibody,molecular modeling was carried out to study the antigen-antibody interaction.The possible antigenic determinant sites on the surface of TCS recognized by both the clones were analyzed,and the reaction forces between TCS and two Fab fragments were also analyzed respectively.

  20. Modeling Stem Cell Induction Processes

    OpenAIRE

    Filipe Grácio; Joaquim Cabral; Bruce Tidor

    2012-01-01

    Technology for converting human cells to pluripotent stem cell using induction processes has the potential to revolutionize regenerative medicine. However, the production of these so called iPS cells is still quite inefficient and may be dominated by stochastic effects. In this work we build mass-action models of the core regulatory elements controlling stem cell induction and maintenance. The models include not only the network of transcription factors NANOG, OCT4, SOX2, but also important e...

  1. Optimal screening of surface-displayed polypeptide libraries.

    Science.gov (United States)

    Boder, E T; Wittrup, K D

    1998-01-01

    Cell surface display of polypeptide libraries combined with flow cytometric cell sorting presents remarkable potential for enhancement of protein-ligand recognition properties. To maximize the utility of this approach, screening and purification conditions must be optimized to take full advantage of the quantitative feature of this technique. In particular, discrimination of improved library mutants from an excess of wild-type polypeptides is dependent upon an effective screening methodology. Fluorescence discrimination profiles for improved library mutants were derived from a mathematical model of expected cell fluorescence intensities for polypeptide libraries screened with fluorescent ligand. Profiles for surface-displayed libraries under equilibrium or kinetic screening conditions demonstrate distinct discrimination optima from which optimal equilibrium and kinetic screening parameters were derived. In addition, a statistical model of low cytometrically analyzed cell populations indicates the importance of low-stringency sorting followed by amplification through regrowth and resorting at increased stringency. This analysis further yields quantitative recommendations for cell-sorting stringency.

  2. Construction and selection of subtracted cDNA library of mouse hepatocarcinoma cell lines with different lymphatic metastasis potential

    Institute of Scientific and Technical Information of China (English)

    Li Hou; Jan-Wu Tang; Xiao-Nan Cui; Bo Wang; Bo Song; Lei Sun

    2004-01-01

    AIM: In order to elucidate the molecular mechanism of lymphatic metastasis of hepatocarcinoma, we detected the difference of gene expression between mouse hepatocarcinoma cell lines Hca-F and Hca-P with different lymphatic metastasis potential.METHODS: cDNA of Hca-F cells was used as a tester and cDNA of Hca-P cells was used as a driver. cDNAs highly expressed in Hca-F cells were isolated by the suppression subtractive hybridization (SSH) method. The isolated cDNA was cloned into T/A cloning vector. The ligation products were transformed into DH5 α competent cells. Individual clones were randomly selected and used for PCR amplification.Vector DNA from positive clones was isolated for sequencing.RESULTS: There were 800 positive clones in amplified subtracted cDNA library. Random analysis of 160 clones with PCR showed that 95% of the clones contained 100-700 bp inserts. Analysis of 20 sequenced cDNA clones randomly picked from the SSH library revealed 4 known genes (mouse heat shock protein 84 ku, DNA helicase, ribosomal protein S13 ,ethanol induced 6 gene) and 3 expressed sequence tags (ESTs). Four cDNAs showed no homology and presumably represent novel genes.CONCLUSION: A subtracted cDNA library of differentially expressed genes in mouse heptocarcinoma cell lines with different lymphatic metastasis potential was successfully constructed with SSH and T/A cloning techniques. The library is efficient and lays a solid foundation for searching new lymphatic metastasis related genes. The expression of mouse heat shock protein gene, DNA helicase and other 4 novel gene may be different between mouse heptocarcinoma cell lines with different lymphatic metastasis potential.

  3. Identification of hypoxia-responsive genes in a dopaminergic cell line by subtractive cDNA libraries and microarray analysis.

    Science.gov (United States)

    Beitner-Johnson, D; Seta, K; Yuan, Y; Kim, H -W.; Rust, R T.; Conrad, P W.; Kobayashi, S; Millhorn, D E.

    2001-07-01

    Transplantation of dopamine-secreting cells harvested from fetal mesencephalon directly into the striatum has had limited success as a therapy for Parkinson's disease. A major problem is that the majority of the cells die during the first 3 weeks following transplantation. Hypoxia in the tissue surrounding the graft is a potential cause of the cell death. We have used subtractive cDNA libraries and microarray analysis to identify the gene expression profile that regulates tolerance to hypoxia. An improved understanding of the molecular basis of hypoxia-tolerance may allow investigators to engineer cells that can survive in the hypoxic environment of the brain parenchyma following transplantation. PMID:11331199

  4. Featured Library: Parrish Library

    OpenAIRE

    Kirkwood, Hal P, Jr

    2015-01-01

    The Roland G. Parrish Library of Management & Economics is located within the Krannert School of Management at Purdue University. Between 2005 - 2007 work was completed on a white paper that focused on a student-centered vision for the Management & Economics Library. The next step was a massive collection reduction and a re-envisioning of both the services and space of the library. Thus began a 3 phase renovation from a 2 floor standard, collection-focused library into a single floor, 18,000s...

  5. Designing Collaborative Developmental Standards by Refactoring of the Earth Science Models, Libraries, Workflows and Frameworks.

    Science.gov (United States)

    Mirvis, E.; Iredell, M.

    2015-12-01

    The operational (OPS) NOAA National Centers for Environmental Prediction (NCEP) suite, traditionally, consist of a large set of multi- scale HPC models, workflows, scripts, tools and utilities, which are very much depending on the variety of the additional components. Namely, this suite utilizes a unique collection of the in-house developed 20+ shared libraries (NCEPLIBS), certain versions of the 3-rd party libraries (like netcdf, HDF, ESMF, jasper, xml etc.), HPC workflow tool within dedicated (sometimes even vendors' customized) HPC system homogeneous environment. This domain and site specific, accompanied with NCEP's product- driven large scale real-time data operations complicates NCEP collaborative development tremendously by reducing chances to replicate this OPS environment anywhere else. The NOAA/NCEP's Environmental Modeling Center (EMC) missions to develop and improve numerical weather, climate, hydrological and ocean prediction through the partnership with the research community. Realizing said difficulties, lately, EMC has been taken an innovative approach to improve flexibility of the HPC environment by building the elements and a foundation for NCEP OPS functionally equivalent environment (FEE), which can be used to ease the external interface constructs as well. Aiming to reduce turnaround time of the community code enhancements via Research-to-Operations (R2O) cycle, EMC developed and deployed several project sub-set standards that already paved the road to NCEP OPS implementation standards. In this topic we will discuss the EMC FEE for O2R requirements and approaches in collaborative standardization, including NCEPLIBS FEE and models code version control paired with the models' derived customized HPC modules and FEE footprints. We will share NCEP/EMC experience and potential in the refactoring of EMC development processes, legacy codes and in securing model source code quality standards by using combination of the Eclipse IDE, integrated with the

  6. A Cell-Based Approach for the Biosynthesis/Screening of Cyclic Peptide Libraries against Bacterial Toxins

    Energy Technology Data Exchange (ETDEWEB)

    Camarero, J A; Kimura, R; Woo, Y; Cantor, J; Steenblock, E

    2007-10-24

    Available methods for developing and screening small drug-like molecules able to knockout toxins or pathogenic microorganisms have some limitations. In order to be useful, these new methods must provide high-throughput analysis and identify specific binders in a short period of time. To meet this need, we are developing an approach that uses living cells to generate libraries of small biomolecules, which are then screened inside the cell for activity. Our group is using this new, combined approach to find highly specific ligands capable of disabling anthrax Lethal Factor (LF) as proof of principle. Key to our approach is the development of a method for the biosynthesis of libraries of cyclic peptides, and an efficient screening process that can be carried out inside the cell.

  7. Cypriot libraries

    OpenAIRE

    John F. Harvey

    1982-01-01

    Describes the current state of librarianship and bibliography in Cyprus, with separate sections for the Greek and Turkish sectors. Although there is no national library in the Greek sector there are 5 types of public library: Nicosia public library; Limassol, Larnaca and Paphos public libraries; community libraries; mobile libraries; and foreign cultural centre libraries. Schools and colleges in the Greek centre are well provided with libraries and most government departments sponsor special ...

  8. Microscopic nuclear models for astrophysics: The Brussels BRUSLIB nuclear library and beyond

    Science.gov (United States)

    Arnould, M.; Goriely, S.

    2006-10-01

    Astrophysics is in need of a broad variety of nuclear data. This concerns static ground state properties, characteristics of excited nuclei, spontaneous decay properties, or interactions of nuclei with (mainly) nucleons, α-particles or photons. A strong theoretical activity complementing laboratory efforts is also mandatory. A large variety of highly ‘exotic’ laboratory-unreachable nuclei are indeed involved in the astrophysics modelling. Even when laboratory-studied nuclei are considered, theory has very often to be called for. Mastering the huge volume of nuclear information and making it available in an accurate and usable form for incorporation into astrophysics models is clearly of pivotal importance. The recognition of this necessity has been the driving motivation for the construction of the Brussels library (BRUSLIB) of computed data of astrophysics relevance. It provides an extended information in tabular form on masses, nuclear level densities and partition functions, fission barriers, and thermonuclear reaction rates. In addition of the unprecedented broadness of its scope, BRUSLIB has the unique and most important feature of relying to the largest possible extent on global and coherent microscopic nuclear models. The models of this sort that we have developed to predict the basic properties of the nuclei and of their interactions are briefly reviewed. The content of the BRUSLIB library that relies on these models is described, as well as a user-friendly nuclear network generator (NETGEN) complementing BRUSLIB. Finally, an application of BRUSLIB and NETGEN to the p-process nucleosynthesis during He detonation in sub-Chandrasekhar CO white dwarfs is proposed.

  9. Microscopic nuclear models for astrophysics: The Brussels BRUSLIB nuclear library and beyond

    Energy Technology Data Exchange (ETDEWEB)

    Arnould, M. [Institut d Astronomie et d Astrophysique, CP 226, Universite Libre de Bruxelles, 1050 Brussels (Belgium)]. E-mail: marnould@astro.ulb.ac.be; Goriely, S. [Institut d Astronomie et d Astrophysique, CP 226, Universite Libre de Bruxelles, 1050 Brussels (Belgium)

    2006-10-17

    Astrophysics is in need of a broad variety of nuclear data. This concerns static ground state properties, characteristics of excited nuclei, spontaneous decay properties, or interactions of nuclei with (mainly) nucleons, {alpha}-particles or photons. A strong theoretical activity complementing laboratory efforts is also mandatory. A large variety of highly 'exotic' laboratory-unreachable nuclei are indeed involved in the astrophysics modelling. Even when laboratory-studied nuclei are considered, theory has very often to be called for. Mastering the huge volume of nuclear information and making it available in an accurate and usable form for incorporation into astrophysics models is clearly of pivotal importance. The recognition of this necessity has been the driving motivation for the construction of the Brussels library (BRUSLIB) of computed data of astrophysics relevance. It provides an extended information in tabular form on masses, nuclear level densities and partition functions, fission barriers, and thermonuclear reaction rates. In addition of the unprecedented broadness of its scope, BRUSLIB has the unique and most important feature of relying to the largest possible extent on global and coherent microscopic nuclear models. The models of this sort that we have developed to predict the basic properties of the nuclei and of their interactions are briefly reviewed. The content of the BRUSLIB library that relies on these models is described, as well as a user-friendly nuclear network generator (NETGEN) complementing BRUSLIB. Finally, an application of BRUSLIB and NETGEN to the p-process nucleosynthesis during He detonation in sub-Chandrasekhar CO white dwarfs is proposed.

  10. Information Society and Library Evaluation Transitions in Portugal: A Meta-evaluation Model and Frameworks (1970–2013

    Directory of Open Access Journals (Sweden)

    Leonor Gaspar Pinto

    2014-02-01

    Full Text Available The need for greater understanding of assessment practices and models highlights a deficit of an up-to-date meta-evaluation model, whilst articulating with new phases in Information Society (IS development. This paper aims to discuss the meta-evaluation model and frameworks that were created to explain the relations between IS transitions and the development of library performance evaluation models in Portugal (1970–2013.The research is based on a qualitative methodology supported by a combination of literature review with the construction and application of conceptual models and frameworks.The meta-evaluation model of the impact of transitions on library performance evaluation provides an adequate representation and explanation of relationships between IS transitions and library performance evaluation models. The CLPET (Categorizing Library Performance Evaluation Typologies Matrix, as well as the relational framework that was developed proved to be useful analytical tools.This paper highlights the current transition of performance models into a more holistic performance management, clarifying that diverse uses and components of performance are strongly linked to IS dynamics. It will also enable readers to discuss the impact of meta-evaluation models as a strong instrument to support the challenges of visions, strategies and best practices shifted over time.

  11. Modeling: driving fuel cells

    Directory of Open Access Journals (Sweden)

    Michael Francis

    2002-05-01

    Fuel cells were invented in 1839 by Sir William Grove, a Welsh judge and gentleman scientist, as a result of his experiments on the electrolysis of water. To put it simply, fuel cells are electrochemical devices that take hydrogen gas from fuel, combine it with oxygen from the air, and generate electricity and heat, with water as the only by-product.

  12. How to Use Benchmark and Cross-section Studies to Improve Data Libraries and Models

    Science.gov (United States)

    Wagner, V.; Suchopár, M.; Vrzalová, J.; Chudoba, P.; Svoboda, O.; Tichý, P.; Krása, A.; Majerle, M.; Kugler, A.; Adam, J.; Baldin, A.; Furman, W.; Kadykov, M.; Solnyshkin, A.; Tsoupko-Sitnikov, S.; Tyutyunikov, S.; Vladimirovna, N.; Závorka, L.

    2016-06-01

    Improvements of the Monte Carlo transport codes and cross-section libraries are very important steps towards usage of the accelerator-driven transmutation systems. We have conducted a lot of benchmark experiments with different set-ups consisting of lead, natural uranium and moderator irradiated by relativistic protons and deuterons within framework of the collaboration “Energy and Transmutation of Radioactive Waste”. Unfortunately, the knowledge of the total or partial cross-sections of important reactions is insufficient. Due to this reason we have started extensive studies of different reaction cross-sections. We measure cross-sections of important neutron reactions by means of the quasi-monoenergetic neutron sources based on the cyclotrons at Nuclear Physics Institute in Řež and at The Svedberg Laboratory in Uppsala. Measurements of partial cross-sections of relativistic deuteron reactions were the second direction of our studies. The new results obtained during last years will be shown. Possible use of these data for improvement of libraries, models and benchmark studies will be discussed.

  13. Self-consistent 2-phase AGN torus models: SED library for observers

    CERN Document Server

    Siebenmorgen, Ralf; Efstathiou, Andreas

    2015-01-01

    We assume that dust near active galactic nuclei (AGN) is distributed in a torus-like geometry, which may be described by a clumpy medium or a homogeneous disk or as a combination of the two (i.e. a 2-phase medium). The dust particles considered are fluffy and have higher submillimeter emissivities than grains in the diffuse ISM. The dust-photon interaction is treated in a fully self-consistent three dimensional radiative transfer code. We provide an AGN library of spectral energy distributions (SEDs). Its purpose is to quickly obtain estimates of the basic parameters of the AGN, such as the intrinsic luminosity of the central source, the viewing angle, the inner radius, the volume filling factor and optical depth of the clouds, and the optical depth of the disk midplane, and to predict the flux at yet unobserved wavelengths. The procedure is simple and consists of finding an element in the library that matches the observations. We discuss the general properties of the models and in particular the 10mic. silic...

  14. Preparing unbiased T cell receptor and antibody cDNA libraries for the deep next generation sequencing profiling

    Directory of Open Access Journals (Sweden)

    Ilgar Z Mamedov

    2013-12-01

    Full Text Available High-throughput sequencing has the power to reveal the nature of adaptive immunity as represented by the full complexity of T cell receptor (TCR and antibody (IG repertoires, but is at present severely compromised by the quantitative bias, bottlenecks, and accumulated errors that inevitably occur in the course of library preparation and sequencing. Here we report an optimized protocol for the unbiased preparation of TCR and IG cDNA libraries for high-throughput sequencing, starting from thousands or millions of live cells in an investigated sample. Critical points to control are revealed, along with tips that allow researchers to minimize quantitative bias, accumulated errors, and cross-sample contamination at each stage, and to enhance the subsequent bioinformatic analysis. The protocol is simple, reliable, and can be performed in 1–2 days.

  15. Variable epitope library carrying heavily mutated survivin-derived CTL epitope variants as a new class of efficient vaccine immunogen tested in a mouse model of breast cancer.

    Science.gov (United States)

    NoeDominguez-Romero, Allan; Zamora-Alvarado, Rubén; Servín-Blanco, Rodolfo; Pérez-Hernández, Erendira G; Castrillon-Rivera, Laura E; Munguia, Maria Elena; Acero, Gonzalo; Govezensky, Tzipe; Gevorkian, Goar; Manoutcharian, Karen

    2014-01-01

    The antigenic variability of tumor cells leading to dynamic changes in cancer epitope landscape along with escape from immune surveillance by down-regulating tumor antigen expression/presentation and immune tolerance are major obstacles for the design of effective vaccines. We have developed a novel concept for immunogen construction based on introduction of massive mutations within the epitopes targeting antigenically variable pathogens and diseases. Previously, we showed that these immunogens carrying large combinatorial libraries of mutated epitope variants, termed as variable epitope libraries (VELs), induce potent, broad and long lasting CD8+IFN-γ+ T-cell response as well as HIV-neutralizing antibodies. In this proof-of-concept study, we tested immunogenic properties and anti-tumor effects of the VELs bearing survivin-derived CTL epitope (GWEPDDNPI) variants in an aggressive metastatic mouse 4T1 breast tumor model. The constructed VELs had complexities of 10,500 and 8,000 individual members, generated as combinatorial M13 phage display and synthetic peptide libraries, respectively, with structural composition GWXPXDXPI, where X is any of 20 natural amino acids. Statistically significant tumor growth inhibition was observed in BALB/c mice immunized with the VELs in both prophylactic and therapeutic settings. Vaccinated mice developed epitope-specific spleen cell and CD8+ IFN-γ+ T-cell responses that recognize more than 50% of the panel of 87 mutated epitope variants, as demonstrated in T-cell proliferation assays and FACS analysis. These data indicate the feasibility of the application of this new class of immunogens based on VEL concept as an alternative approach for the development of molecular vaccines against cancer.

  16. Reference Services in Australian Academic Libraries are Becoming More Multifaceted, A Review of: Burke, L. “Models of Reference Services in Australian Academic Libraries.” Journal of Librarianship and Information Science 40.4 (2008): 269‐86.

    OpenAIRE

    David Herron; Lotta Haglund

    2009-01-01

    Objective – To investigate the current organizational models for reference work in Australian academic libraries, and how these reference services are staffed.Design – Mixed methods.Setting – Academic universities in Australia.Subjects – Forty Council of Australian University Librarians (CAUL) member libraries.Methods – A literature study was undertaken to (1) find a definition of reference services and (2) explore the development of reference service models over time. Statistics from the CAU...

  17. Stochastic models of cell motility

    DEFF Research Database (Denmark)

    Gradinaru, Cristian

    2012-01-01

    Cell motility and migration are central to the development and maintenance of multicellular organisms, and errors during this process can lead to major diseases. Consequently, the mechanisms and phenomenology of cell motility are currently under intense study. In recent years, a new...... interdisciplinary field focusing on the study of biological processes at the nanoscale level, with a range of technological applications in medicine and biological research, has emerged. The work presented in this thesis is at the interface of cell biology, image processing, and stochastic modeling. The stochastic...... models introduced here are based on persistent random motion, which I apply to real-life studies of cell motility on flat and nanostructured surfaces. These models aim to predict the time-dependent position of cell centroids in a stochastic manner, and conversely determine directly from experimental...

  18. Single-stranded DNA library preparation uncovers the origin and diversity of ultrashort cell-free DNA in plasma

    Science.gov (United States)

    Burnham, Philip; Kim, Min Seong; Agbor-Enoh, Sean; Luikart, Helen; Valantine, Hannah A.; Khush, Kiran K.; De Vlaminck, Iwijn

    2016-01-01

    Circulating cell-free DNA (cfDNA) is emerging as a powerful monitoring tool in cancer, pregnancy and organ transplantation. Nucleosomal DNA, the predominant form of plasma cfDNA, can be adapted for sequencing via ligation of double-stranded DNA (dsDNA) adapters. dsDNA library preparations, however, are insensitive to ultrashort, degraded cfDNA. Drawing inspiration from advances in paleogenomics, we have applied a single-stranded DNA (ssDNA) library preparation method to sequencing of cfDNA in the plasma of lung transplant recipients (40 samples, six patients). We found that ssDNA library preparation yields a greater portion of sub-100 bp nuclear genomic cfDNA (p 10−5, Mann-Whitney U Test), and an increased relative abundance of mitochondrial (10.7x, p 10−5) and microbial cfDNA (71.3x, p 10−5). The higher yield of microbial sequences from this method increases the sensitivity of cfDNA-based monitoring for infections following transplantation. We detail the fragmentation pattern of mitochondrial, nuclear genomic and microbial cfDNA over a broad fragment length range. We report the observation of donor-specific mitochondrial cfDNA in the circulation of lung transplant recipients. A ssDNA library preparation method provides a more informative window into understudied forms of cfDNA, including mitochondrial and microbial derived cfDNA and short nuclear genomic cfDNA, while retaining information provided by standard dsDNA library preparation methods. PMID:27297799

  19. Single-stranded DNA library preparation uncovers the origin and diversity of ultrashort cell-free DNA in plasma.

    Science.gov (United States)

    Burnham, Philip; Kim, Min Seong; Agbor-Enoh, Sean; Luikart, Helen; Valantine, Hannah A; Khush, Kiran K; De Vlaminck, Iwijn

    2016-01-01

    Circulating cell-free DNA (cfDNA) is emerging as a powerful monitoring tool in cancer, pregnancy and organ transplantation. Nucleosomal DNA, the predominant form of plasma cfDNA, can be adapted for sequencing via ligation of double-stranded DNA (dsDNA) adapters. dsDNA library preparations, however, are insensitive to ultrashort, degraded cfDNA. Drawing inspiration from advances in paleogenomics, we have applied a single-stranded DNA (ssDNA) library preparation method to sequencing of cfDNA in the plasma of lung transplant recipients (40 samples, six patients). We found that ssDNA library preparation yields a greater portion of sub-100 bp nuclear genomic cfDNA (p 10(-5), Mann-Whitney U Test), and an increased relative abundance of mitochondrial (10.7x, p 10(-5)) and microbial cfDNA (71.3x, p 10(-5)). The higher yield of microbial sequences from this method increases the sensitivity of cfDNA-based monitoring for infections following transplantation. We detail the fragmentation pattern of mitochondrial, nuclear genomic and microbial cfDNA over a broad fragment length range. We report the observation of donor-specific mitochondrial cfDNA in the circulation of lung transplant recipients. A ssDNA library preparation method provides a more informative window into understudied forms of cfDNA, including mitochondrial and microbial derived cfDNA and short nuclear genomic cfDNA, while retaining information provided by standard dsDNA library preparation methods. PMID:27297799

  20. Complementation of radiation-sensitive Ataxia telangiectasia cells after transfection of cDNA expression libraries and cosmid clones from wildtype cells

    International Nuclear Information System (INIS)

    In this Ph.D.-thesis, phenotypic complementation of AT-cells (AT5BIVA) by transfection of cDNA-expression-libraries was adressed: After stable transfection of cDNA-expression-libraries G418 resistant clones were selected for enhanced radioresistance by a fractionated X-ray selection. One surviving transfectant clone (clone 514) exhibited enhanced radiation resistance in dose-response experiments and further X-ray selections. Cell cycle analysis revealed complementation of untreated and irradiated 514-cells in cell cycle progression. The rate of DNA synthesis, however, is not diminished after irradiation but shows the reverse effect. A transfected cDNA-fragment (AT500-cDNA) was isolated from the genomic DNA of 514-cells and proved to be an unknown DNA sequence. A homologous sequence could be detected in genomic DNA from human cell lines, but not in DNA from other species. The cDNA-sequence could be localized to human chromosome 11. In human cells the cDNA sequence is part of two large mRNAs. 4 different cosmid clones containing high molecular genomic DNA from normal human cells could be isolated from a library, each hybridizing to the AT500-cDNA. After stable transfection into AT-cells, one cosmid-clone was able to confer enhanced radiation resistance both in X-ray selections and dose-response experiments. The results indicate that the cloned cDNA-fragment is based on an unknown gene from human chromosome 11 which partially complements the radiosensitivity and the defective cell cycle progression in AT5BIVA cells. (orig.)

  1. Construction and sequence sampling of deep-coverage, large-insert BAC libraries for three model lepidopteran species

    Directory of Open Access Journals (Sweden)

    Zhao Shaying

    2009-06-01

    Full Text Available Abstract Background Manduca sexta, Heliothis virescens, and Heliconius erato represent three widely-used insect model species for genomic and fundamental studies in Lepidoptera. Large-insert BAC libraries of these insects are critical resources for many molecular studies, including physical mapping and genome sequencing, but not available to date. Results We report the construction and characterization of six large-insert BAC libraries for the three species and sampling sequence analysis of the genomes. The six BAC libraries were constructed with two restriction enzymes, two libraries for each species, and each has an average clone insert size ranging from 152–175 kb. We estimated that the genome coverage of each library ranged from 6–9 ×, with the two combined libraries of each species being equivalent to 13.0–16.3 × haploid genomes. The genome coverage, quality and utility of the libraries were further confirmed by library screening using 6~8 putative single-copy probes. To provide a first glimpse into these genomes, we sequenced and analyzed the BAC ends of ~200 clones randomly selected from the libraries of each species. The data revealed that the genomes are AT-rich, contain relatively small fractions of repeat elements with a majority belonging to the category of low complexity repeats, and are more abundant in retro-elements than DNA transposons. Among the species, the H. erato genome is somewhat more abundant in repeat elements and simple repeats than those of M. sexta and H. virescens. The BLAST analysis of the BAC end sequences suggested that the evolution of the three genomes is widely varied, with the genome of H. virescens being the most conserved as a typical lepidopteran, whereas both genomes of H. erato and M. sexta appear to have evolved significantly, resulting in a higher level of species- or evolutionary lineage-specific sequences. Conclusion The high-quality and large-insert BAC libraries of the insects, together

  2. Mathematical Model for Photovoltaic Cells

    OpenAIRE

    Wafaa ABD EL-BASIT; Ashraf Mosleh ABD El–MAKSOOD; Fouad Abd El-Moniem Saad SOLIMAN

    2013-01-01

    The study of photovoltaic systems in an efficient manner requires a precise knowledge of the (I-V) and (P-V) characteristic curves of photovoltaic modules. So, the aim of the present paper is to estimate such characteristics based on different operating conditions. In this concern, a simple one diode mathematical model was implemented using MATLAB script. The output characteristics of PV cell depend on the environmental conditions. For any solar cell, the model parameters are function of the ...

  3. A novel peptide, selected from phage display library of random peptides, can efficiently target into human breast cancer cell

    Institute of Scientific and Technical Information of China (English)

    DONG Jian; LIU WeiQing; JIANG AiMei; ZHANG KeJian; CHEN MingQing

    2008-01-01

    To develop a targeting vector for breast cancer biotherapy, MDA-MB-231 cell, a human breast cancer cell line, was co-cultured with pC89 (9 aa) phage display library of random peptides. In multiple inde-pendent peptide-presenting phage screening trials, subtilisin was used as a protease to inactivate ex-tra-cellular phages. The internalized phages were collected by cell lysising and amplified in E. coli XLI-Blue. Through five rounds of selection, the peptide-presenting phages which could be internalized in MDA-MB-231 cells were isolated. A comparison was made between internalization capacities of pep-tide-presenting phages isolated from MDA-MB-231 cells and RGD-integrin binding phage by cocultur-ing them with other human tumor cell lines and normal cells. The nucleotide sequences of isolated peptide-presenting phages were then determined by DNA sequencing. To uncover whether phage coat protein or amino acid order was required for the character of the peptide to MDA-MB-231 cells, three peptides were synthesized. They are CASPSGALRSC, ASPSGALRS and CGVIFDHSVPC (the shifted sequence of CASPSGALRSC), and after coculturing them with different cell lines, their targeting ca-pacities to MDA-MB-231 cells were detected. These data suggested that the internalization process was highly selective, and capable of capturing a specific peptide from parent peptide variants. Moreover, the targeting internalization event of peptides was an amino acid sequence dependent manner. The results demonstrated the feasibility of using phage display library of random peptides to develop new targeting system for intracellular delivery of macromolecules, and the peptide we obtained might be modified as a targeting vector for breast cancer gene therapy.

  4. Screening and Identification of a Novel Hepatocellular Carcinoma Cell Binding Peptide by Using a Phage Display Library

    Institute of Scientific and Technical Information of China (English)

    Xiaohua ZHU; Hua WU; Sha LUO; Zhiqun XIANYU; Dan ZHU

    2008-01-01

    The purpose of this study was to screen peptides that can specifically bind to human hepatocellular carcinoma (hHCC) cells using phage display of random peptide library in order to develope a peptide-based carrier for the diagnosis or therapy of hHCC. A peptide 12-mer phage display library was employed and 4 rounds of subtractive panning were performed using the hHCC cell line HepG2 as the target. After panning, the phages that specifically bound to and internalized in hHCC cells were selected. The selected phages demonstrated highly specific affinity to HepG2 cells analyzed by ELISA and immunofluorescence analysis. 57.3% of the selected phage clones displayed repeated sequence FLLEPHLMDTSM, and 4 amino acid residues, FLEP were extremely conservative. Based on the sequencing results, a 16-mer peptide (WH-16) was synthesized. The competitive ELISA showed that the binding of the phage clones displayed sequence FLLEPHLMDTSM to HepG2 cells was efficiently inhibited by WH-16. Our findings indicate that cellular binding of phage is mediated via its displayed peptide and the synthesized 16-mer peptide may have the potential to be a delivery Carrier in target diagnosis or therapy for hHCC.

  5. Stellar population synthesis models between 2.5 and 5 {\\mu}m based on the empirical IRTF stellar library

    OpenAIRE

    Röck, B.; Vazdekis, A.; Peletier, R.F.; Knapen, J. H.; Falcón-Barroso, J.

    2015-01-01

    We present the first single-burst stellar population models in the infrared wavelength range between 2.5 and 5 {\\mu}m which are exclusively based on empirical stellar spectra. Our models take as input 180 spectra from the stellar IRTF (Infrared Telescope Facility) library. Our final single-burst stellar population models are calculated based on two different sets of isochrones and various types of initial mass functions of different slopes, ages larger than 1 Gyr and metallicities between [Fe...

  6. Conceptualising 'knowledge management' in the context of library and information science using the core/periphery model

    Directory of Open Access Journals (Sweden)

    O.B. Onyancha

    2009-04-01

    Full Text Available This study took cognisance of the fact that the term 'knowledge management' lacks a universally accepted definition, and consequently sought to describe the term using the most common co-occurring terms in knowledge management (KM literature as indexed in the Library, Information Science and Technology Abstracts (LISTA database. Using a variety of approaches and analytic techniques (e.g. core/periphery analysis and co-occurrence of words as subject terms, data were analysed using the core/periphery model and social networks through UCINET for Windows, TI, textSTAT and Bibexcel computer-aided software. The study identified the following as the compound terms with which KM co-occurs most frequently: information resources management, information science, information technology, information services, information retrieval, library science, management information systems and libraries. The core single subject terms with which KM can be defined include resources, technology, libraries, systems, services, retrieval, storage, data and computers. The article concludes by offering the library and information science (LIS professionals' general perception of KM based on their use of terms, through which KM can be defined within the context of LIS.

  7. Model cell membranes

    DEFF Research Database (Denmark)

    Günther-Pomorski, Thomas; Nylander, Tommy; Cardenas Gomez, Marite

    2014-01-01

    The high complexity of biological membranes has motivated the development and application of a wide range of model membrane systems to study biochemical and biophysical aspects of membranes in situ under well defined conditions. The aim is to provide fundamental understanding of processes control...

  8. Library Publishing is Special: Selection and Eligibility in Library Publishing

    OpenAIRE

    Royster, Paul

    2014-01-01

    Traditional publishing is based on ownership, commerce, paid exchanges, and scholarship as a commodity, while library activities are based on a service model of sharing resources and free exchange. I believe library publishing should be based on those values and should not duplicate or emulate traditional publishing. University presses have mixed views of library publishing, and libraries should not adopt those attitudes. Library publishers are not gatekeepers; their mission is dissemination....

  9. Democratizing Human Genome Project Information: A Model Program for Education, Information and Debate in Public Libraries.

    Science.gov (United States)

    Pollack, Miriam

    The "Mapping the Human Genome" project demonstrated that librarians can help whomever they serve in accessing information resources in the areas of biological and health information, whether it is the scientists who are developing the information or a member of the public who is using the information. Public libraries can guide library users…

  10. A Basic Hybrid Library Support Model to Distance Learners in Sudan

    Science.gov (United States)

    Abdelrahman, Omer Hassan

    2012-01-01

    Distance learning has flourished in Sudan during the last two decades; more and more higher education institutions offer distance learning programmes to off-campus students. Like on-campus students, distance learners should have access to appropriate library and information support services. They also have specific needs for library and…

  11. A Model for Managing 3D Printing Services in Academic Libraries

    Science.gov (United States)

    Scalfani, Vincent F.; Sahib, Josh

    2013-01-01

    The appearance of 3D printers in university libraries opens many opportunities for advancing outreach, teaching, and research programs. The University of Alabama (UA) Libraries recently adopted 3D printing technology and maintains an open access 3D Printing Studio. The Studio consists of a 3D printer, multiple 3D design workstations, and other…

  12. Accessibility, Collaboration, and Staffing: Revamping the Model for Academic Library Video Collections

    Science.gov (United States)

    McGeary, Bryan James

    2015-01-01

    This column contains information on how students in graduate library and information science programs participate in a forum to discuss key issues they see in academic library public services, to envision what they feel librarians in public service have to offer to academia, to state their visions for the profession, or to tell of research that is…

  13. 图书馆移动服务模式研究%Research on the Mobile Service Model of Libraries

    Institute of Scientific and Technical Information of China (English)

    谢强; 牛现云; 赵娜; 高宏

    2012-01-01

    目前,图书馆移动服务主要实现方式为短(彩)信方式、基于浏览器的服务方式、客户端(移动)应用程序,服务内容主要有续借、预约等图书流通相关服务及信息公告、移动阅读等。但是无论从实现技术还是服务内容来看,服务模式都具有一定的局限性。因此,从发展角度及广泛适用性来看,图书馆移动服务模式应该包括延伸型图书馆移动服务、单一型图书馆移动服务、融合型图书馆移动服务。%At present,mobile services of libraries are mainly implemented by short(multimedia) message services,browser-based services and the client Application(APP).The mobile service content of libraries primarily includes book circulation services such as renew and booking,information bulletin,mobile reading,and so on.However,no matter in the view of implementation technique or service content,there are certain limitations to mobile service models.The mobile service model of libraries should be considered from development perspective,as well as extensive applicability,it should include extended type mobile services of libraries,single type mobile services of libraries,mobile services of libraries based on the network convergence.

  14. Physical models of cell motility

    CERN Document Server

    2016-01-01

    This book surveys the most recent advances in physics-inspired cell movement models. This synergetic, cross-disciplinary effort to increase the fidelity of computational algorithms will lead to a better understanding of the complex biomechanics of cell movement, and stimulate progress in research on related active matter systems, from suspensions of bacteria and synthetic swimmers to cell tissues and cytoskeleton.Cell motility and collective motion are among the most important themes in biology and statistical physics of out-of-equilibrium systems, and crucial for morphogenesis, wound healing, and immune response in eukaryotic organisms. It is also relevant for the development of effective treatment strategies for diseases such as cancer, and for the design of bioactive surfaces for cell sorting and manipulation. Substrate-based cell motility is, however, a very complex process as regulatory pathways and physical force generation mechanisms are intertwined. To understand the interplay between adhesion, force ...

  15. Approach to General Data Model of GIS Symbol Library and Symbol Library Data Exchange XML Schema%GIS符号库通用数据模型及其数据交换格式的研究

    Institute of Scientific and Technical Information of China (English)

    李青元; 苏德国; 李洪省; 刘皓晨; 孙立坚

    2009-01-01

    Symbol portrayal is an important function of GIS.Sharing symbolic information in different GIS platforms is neces-sary for GIS applications and users.This paper discusses the necessity,possibility and solution technique of sharing a symbol library in different GIS platforms.The route map is designed as follows:first,to set up a general data model for the symbol li-brary,then to design a standard exchange format,and finally to call on the GIS manufacturer to provide the interchange tools for their symbol library for the standard exchange format.This paper analyzes the general characteristics of GIS symbolic li-brary,gives a symbol library model and a draft of XML schema of the symbol library exchange format.

  16. A distributed, collaborative, structuring model for a clinical-guideline digital-library.

    Science.gov (United States)

    Shahar, Yuval; Shalom, Erez; Mayaffit, Alon; Young, Ohad; Galperin, Maya; Martins, Susana; Goldstein, Mary

    2003-01-01

    The Digital Electronic Guideline Library (DeGeL) is a Web-based framework and a set of distributed tools that facilitate gradual conversion of clinical guidelines from free text, through semi-structured text, to a fully structured, executable representation. Thus, guidelines exist in a hybrid, multiple-format representation The three formats support increasingly sophisticated computational tasks. The tools perform semantic markup, classification, search, and browsing, and support computational modules that we are developing, for run-time application and retrospective quality assessment. We describe the DeGeL architecture and its collaborative-authoring authorization model, which is based on (1) multiple medical-specialty authoring groups, each including a group manager who controls group authorizations, and (2) a hierarchical authorization model based on the different functions involved in the hybrid guideline-specification process. We have implemented the core modules of the DeGeL architecture and demonstrated distributed markup and retrieval using the knowledge roles of two guidelines ontologies (Asbru and GEM). We are currently evaluating several of the DeGeL tools.

  17. Rethinking the data model: the drillbit proof-of-concept library

    International Nuclear Information System (INIS)

    The focus of many software architectures of the LHC experiments is to deliver a well-designed Event Data Model (EDM). Changes and additions to the stored data are often expensive, requiring large amounts of CPU time, disk storage and man-power. In addition, differing needs between groups of physicists lead to a tendency for common data formats to grow in terms of contained information whilst still not managing to service all needs. We introduce a new way of thinking about the data model based on the Dremel column store architecture published by Google. We present an EDM concept based on Dremel, which has the potential to significantly reduce the storage requirement for these common formats, decrease the time needed for independent physicists to compare their results and improve the speed at which data reprocessings can feasibly take place. The Dremel low-level encoding is implemented in a proof-of-concept C++ library called Drillbit, and it is shown that using a different encoding of the current data could save as much as 20% of disk space on average across a wide number of real-world derived data sets.

  18. Library system of Italy

    Directory of Open Access Journals (Sweden)

    Nataša Gerbec

    2003-01-01

    Full Text Available In the European extent, Italy is the cradle of libraries and library sciences. In the past, Italian national public libraries played an important role through their vast book treasury. But only during the last thirty years have public libraries been developed following the Anglo-American public library model. Italy does not have any uniform or general legislation concerning libraries. On the state level, this area is regulated by some separate acts, while on the regional level there is a collection of various acts and regulations. Libraries are not strictly divided into general categories. It is required that the professionals engaged in Italian libraries should have secondary or university education. The level of their professional tasks depends on the type of library and its capacity. The competency for the development in the field of librarianship is assigned to The Ministry of Cultural and Environment Heritage as well as to its subordinate institutions (Central Institute for the Union catalogue of Italian Libraries and for Bibliographic Information, Central Institute for Book Pathology, Observatory for International Libraries Programmes.

  19. The Modeling Library of Eavesdropping Methods in Quantum Cryptography Protocols by Model Checking

    Science.gov (United States)

    Yang, Fan; Yang, Guowu; Hao, Yujie

    2016-07-01

    The most crucial issue of quantum cryptography protocols is its security. There exists many ways to attack the quantum communication process. In this paper, we present a model checking method for modeling the eavesdropping in quantum information protocols. So when the security properties of a certain protocol are needed to be verified, we can directly use the models which are already built. Here we adopt the probabilistic model checking tool—PRISM to model these attack methods. The verification results show that the detection rate of eavesdropping is approximately close to 1 when enough photons are transmitted.

  20. Library Automation

    OpenAIRE

    Dhakne, B. N.; Giri, V. V; Waghmode, S. S.

    2010-01-01

    New technologies library provides several new materials, media and mode of storing and communicating the information. Library Automation reduces the drudgery of repeated manual efforts in library routine. By use of library automation collection, Storage, Administration, Processing, Preservation and communication etc.

  1. Selected Aspects Of Modelling Of Non-Linear Behaviour Of Concrete During Tensile Test Using Multiplas Library

    Directory of Open Access Journals (Sweden)

    Hokeš Filip

    2015-12-01

    Full Text Available The subject of this paper is to describe some of the aspects manifesting in the use of the elastoplastic material model library multiPlas, which was developed to support non-linear computations in the ANSYS system. The text focuses on the analysis of numerical simulations of a virtual tension test in several case studies, thereby the text endeavours to describe the problems connected with modelling non-linear behaviour of concrete in a tensile area.

  2. The Human Glioblastoma Cell Culture Resource: Validated Cell Models Representing All Molecular Subtypes

    Directory of Open Access Journals (Sweden)

    Yuan Xie

    2015-10-01

    Full Text Available Glioblastoma (GBM is the most frequent and malignant form of primary brain tumor. GBM is essentially incurable and its resistance to therapy is attributed to a subpopulation of cells called glioma stem cells (GSCs. To meet the present shortage of relevant GBM cell (GC lines we developed a library of annotated and validated cell lines derived from surgical samples of GBM patients, maintained under conditions to preserve GSC characteristics. This collection, which we call the Human Glioblastoma Cell Culture (HGCC resource, consists of a biobank of 48 GC lines and an associated database containing high-resolution molecular data. We demonstrate that the HGCC lines are tumorigenic, harbor genomic lesions characteristic of GBMs, and represent all four transcriptional subtypes. The HGCC panel provides an open resource for in vitro and in vivo modeling of a large part of GBM diversity useful to both basic and translational GBM research.

  3. The Human Glioblastoma Cell Culture Resource: Validated Cell Models Representing All Molecular Subtypes.

    Science.gov (United States)

    Xie, Yuan; Bergström, Tobias; Jiang, Yiwen; Johansson, Patrik; Marinescu, Voichita Dana; Lindberg, Nanna; Segerman, Anna; Wicher, Grzegorz; Niklasson, Mia; Baskaran, Sathishkumar; Sreedharan, Smitha; Everlien, Isabelle; Kastemar, Marianne; Hermansson, Annika; Elfineh, Lioudmila; Libard, Sylwia; Holland, Eric Charles; Hesselager, Göran; Alafuzoff, Irina; Westermark, Bengt; Nelander, Sven; Forsberg-Nilsson, Karin; Uhrbom, Lene

    2015-10-01

    Glioblastoma (GBM) is the most frequent and malignant form of primary brain tumor. GBM is essentially incurable and its resistance to therapy is attributed to a subpopulation of cells called glioma stem cells (GSCs). To meet the present shortage of relevant GBM cell (GC) lines we developed a library of annotated and validated cell lines derived from surgical samples of GBM patients, maintained under conditions to preserve GSC characteristics. This collection, which we call the Human Glioblastoma Cell Culture (HGCC) resource, consists of a biobank of 48 GC lines and an associated database containing high-resolution molecular data. We demonstrate that the HGCC lines are tumorigenic, harbor genomic lesions characteristic of GBMs, and represent all four transcriptional subtypes. The HGCC panel provides an open resource for in vitro and in vivo modeling of a large part of GBM diversity useful to both basic and translational GBM research. PMID:26629530

  4. Mathematical Model for Photovoltaic Cells

    Directory of Open Access Journals (Sweden)

    Wafaa ABD EL-BASIT

    2013-11-01

    Full Text Available The study of photovoltaic systems in an efficient manner requires a precise knowledge of the (I-V and (P-V characteristic curves of photovoltaic modules. So, the aim of the present paper is to estimate such characteristics based on different operating conditions. In this concern, a simple one diode mathematical model was implemented using MATLAB script. The output characteristics of PV cell depend on the environmental conditions. For any solar cell, the model parameters are function of the irradiance and the temperature values of the site where the panel is placed. In this paper, the numerical values of the equivalent circuit parameters are generated by the program. As well, the dependence of the cells electrical parameters are analyzed under the influence of different irradiance and temperature levels. The variation of slopes of the (I–V curves of a cell at short-circuit and open-circuit conditions with intensity of illumination in small span of intensity and different temperature levels have been applied to determine the cell parameters, shunt resistance, series resistance. The results show that the efficiency of solar cells has an inverse relationship with temperature, irradiance levels are affected by the change of the photo-generation current and the series resistance in the single diode model.

  5. A multipurpose vector system for the screening of libraries in bacteria, insect and mammalian cells and expression in vivo

    OpenAIRE

    Laitinen, Olli H.; Airenne, Kari J; Hytönen, Vesa P; Peltomaa, Erik; Mähönen, Anssi J.; Wirth, Thomas; Lind, Miia M.; Mäkelä, Kari A.; Toivanen, Pyry I.; Schenkwein, Diana; Heikura, Tommi; Nordlund, Henri R.; Kulomaa, Markku S.; Ylä-Herttuala, Seppo

    2005-01-01

    We have constructed a novel tetra-promoter vector (pBVboostFG) system that enables screening of gene/cDNA libraries for functional genomic studies. The vector enables an all-in-one strategy for gene expression in mammalian, bacterial and insect cells and is also suitable for direct use in vivo. Virus preparation is based on an improved mini Tn7 transpositional system allowing easy and fast production of recombinant baculoviruses with high diversity and negligible background. Cloning of the de...

  6. Epigenetic Library Screen Identifies Abexinostat as Novel Regulator of Adipocytic and Osteoblastic Differentiation of Human Skeletal (Mesenchymal) Stem Cells

    DEFF Research Database (Denmark)

    Ali; Hamam; Alfayez;

    2016-01-01

    The epigenetic mechanisms promoting lineage-specific commitment of human skeletal (mesenchymal or stromal) stem cells (hMSCs) into adipocytes or osteoblasts are still not fully understood. Herein, we performed an epigenetic library functional screen and identified several novel compounds, including...... abexinostat, which promoted adipocytic and osteoblastic differentiation of hMSCs. Using gene expression microarrays, chromatin immunoprecipitation for H3K9Ac combined with high-throughput DNA sequencing (ChIP-seq), and bioinformatics, we identified several key genes involved in regulating stem cell...... or stromal) stem cells (hMSCs). These data provide new insight into the understanding of the relationship between the epigenetic effect of histone deacetylase inhibitors, transcription factors, and differentiation pathways controlling adipocyte and osteoblast differentiation of hMSCs. Manipulating...

  7. Experience in Computer-Assisted XML-Based Modelling in the Context of Libraries

    CERN Document Server

    Niinimäki, M

    2003-01-01

    In this paper, we introduce a software called Meta Data Visualisation (MDV) that (i) assists the user with a graphical user interface in the creation of his specific document types, (ii) creates a database according to these document types, (iii) allows the user to browse the database, and (iv) uses native XML presentation of the data in order to allow queries or data to be exported to other XML-based systems. We illustrate the use of MDV and XML modelling using library-related examples to build a bibliographic database. In our opinion, creating document type descriptions corresponds to conceptual and logical database design in a database design process. We consider that this design can be supported with a suitable set of tools that help the designer concentrate on conceptual issues instead of implementation issues. Our hypothesis is that using the methodology presented in this paper we can create XML databases that are useful and relevant, and with which MDV works as a user interface.

  8. Relevance feedback and query expansion for searching the web: a model for searching a digital library

    OpenAIRE

    Alan F. SMEATON; Crimmins, Francis

    1997-01-01

    A fully operational large scale digital library is likely to be based on a distributed architecture and because of this it is likely that a number of independent search engines may be used to index different overlapping portions of the entire contents of the library. In any case, different media, text, audio, image, etc., will be indexed for retrieval by different search engines so techniques which provide a coherent and unified search over a suite of underlying independent search engines are...

  9. A Prototype System for a Computer-Based Statewide Film Library Network: A Model for Operation. Number 3, Statewide Film Library Network: System Write-Up.

    Science.gov (United States)

    Auricchio, Dominick

    An overview of materials scheduling, this write-up outlines system components, standardization, costs, limitations, and expansion capabilities of the New York Statewide Film Library Network. Interacting components include research staff; materials libraries; hardware; input/output (operation modes, input format conventions, transaction codes);…

  10. An efficient current-based logic cell model for crosstalk delay analysis

    Science.gov (United States)

    Nazarian, Shahin; Das, Debasish

    2013-04-01

    Logic cell modelling is an important component in the analysis and design of CMOS integrated circuits, mostly due to nonlinear behaviour of CMOS cells with respect to the voltage signal at their input and output pins. A current-based model for CMOS logic cells is presented, which can be used for effective crosstalk noise and delta delay analysis in CMOS VLSI circuits. Existing current source models are expensive and need a new set of Spice-based characterisation, which is not compatible with typical EDA tools. In this article we present Imodel, a simple nonlinear logic cell model that can be derived from the typical cell libraries such as NLDM, with accuracy much higher than NLDM-based cell delay models. In fact, our experiments show an average error of 3% compared to Spice. This level of accuracy comes with a maximum runtime penalty of 19% compared to NLDM-based cell delay models on medium-sized industrial designs.

  11. National Policy on Public Libraries in India.

    Science.gov (United States)

    Jambhekar, Neeta

    1995-01-01

    Investigates the government policies in India, especially the five-year plans regarding finance, since its independence in 1947 and their impact on the establishment of the public library system. Highlights include model library systems, library development, national libraries, information services, and adult education and public libraries.…

  12. Cloning vectors for expression of cDNA libraries in mammalian cells.

    OpenAIRE

    Murphy, A.J.; Efstratiadis, A

    1987-01-01

    We have constructed a series of compound cloning vectors (lambda ZD vectors), each consisting of phage lambda arms carrying a modified version of the retroviral expression vector pZIP-neoSV (x)1. cDNA, inserted into a cloning site present in the retroviral vector component, is cloned with high efficiency using the lambda system. A cDNA library in plasmids is then released by homologous recombination between the retroviral long terminal repeats. Retroviral transduction is achieved by transient...

  13. Chum-RNA allows preparation of a high-quality cDNA library from a single-cell quantity of mRNA without PCR amplification.

    Science.gov (United States)

    Tougan, Takahiro; Okuzaki, Daisuke; Nojima, Hiroshi

    2008-09-01

    Linear RNA amplification using T7 RNA polymerase is useful in genome-wide analysis of gene expression using DNA microarrays, but exponential amplification using polymerase chain reaction (PCR) is still required for cDNA library preparation from single-cell quantities of RNA. We have designed a small RNA molecule called chum-RNA that has enabled us to prepare a single-cell cDNA library after four rounds of T7-based linear amplification, without using PCR amplification. Chum-RNA drove cDNA synthesis from only 0.49 femtograms of mRNA (730 mRNA molecules) as a substrate, a quantity that corresponds to a minor population of mRNA molecules in a single mammalian cell. Analysis of the independent cDNA clone of this library (6.6 x 10(5) cfu) suggests that 30-fold RNA amplification occurred in each round of the amplification process. The size distribution and representation of mRNAs in the resulting one-cell cDNA library retained its similarity to that of the million-cell cDNA library. The use of chum-RNA might also facilitate reactions involving other DNA/RNA modifying enzymes whose Michaelis constant (K(m)) values are around 1 mM, allowing them to be activated in the presence of only small quantities of substrate. PMID:18603591

  14. A Scalable Cloud Library Empowering Big Data Management, Diagnosis, and Visualization of Cloud-Resolving Models

    Science.gov (United States)

    Zhou, S.; Tao, W. K.; Li, X.; Matsui, T.; Sun, X. H.; Yang, X.

    2015-12-01

    A cloud-resolving model (CRM) is an atmospheric numerical model that can numerically resolve clouds and cloud systems at 0.25~5km horizontal grid spacings. The main advantage of the CRM is that it can allow explicit interactive processes between microphysics, radiation, turbulence, surface, and aerosols without subgrid cloud fraction, overlapping and convective parameterization. Because of their fine resolution and complex physical processes, it is challenging for the CRM community to i) visualize/inter-compare CRM simulations, ii) diagnose key processes for cloud-precipitation formation and intensity, and iii) evaluate against NASA's field campaign data and L1/L2 satellite data products due to large data volume (~10TB) and complexity of CRM's physical processes. We have been building the Super Cloud Library (SCL) upon a Hadoop framework, capable of CRM database management, distribution, visualization, subsetting, and evaluation in a scalable way. The current SCL capability includes (1) A SCL data model enables various CRM simulation outputs in NetCDF, including the NASA-Unified Weather Research and Forecasting (NU-WRF) and Goddard Cumulus Ensemble (GCE) model, to be accessed and processed by Hadoop, (2) A parallel NetCDF-to-CSV converter supports NU-WRF and GCE model outputs, (3) A technique visualizes Hadoop-resident data with IDL, (4) A technique subsets Hadoop-resident data, compliant to the SCL data model, with HIVE or Impala via HUE's Web interface, (5) A prototype enables a Hadoop MapReduce application to dynamically access and process data residing in a parallel file system, PVFS2 or CephFS, where high performance computing (HPC) simulation outputs such as NU-WRF's and GCE's are located. We are testing Apache Spark to speed up SCL data processing and analysis.With the SCL capabilities, SCL users can conduct large-domain on-demand tasks without downloading voluminous CRM datasets and various observations from NASA Field Campaigns and Satellite data to a

  15. The Study of Analytical Model of Library Electronic Resources Usage-A Case of Medical Electronic Resources

    Directory of Open Access Journals (Sweden)

    Chung-Yen Yu

    2014-10-01

    Full Text Available With the advents of internet, the importance of electronic resources is growing. Due to the increasing expensiveness of electronic resources, university libraries normally received budgets from parent institutions annually. They necessarily applied effective and systematic methods for decision making in electronic resources purchase or re-subscription. However, there are some difficulties in practices: First of all, libraries are unable to receive user records; second, the COUNTER statistics does not include details about users and their affiliation. As a result, one cannot conduct advanced user analysis based on the usage of users, institutions, and departments. To overcome the difficulties, this study presents a feasible model to analyze electronic resource usage effectively and flexibly. We set up a proxy server to collect actual usage raw data. By analyzing items in internet browsing records, associated with original library automatic system, this study aims at exploring how to use effective ways to analyze big data of website log data. We also propose the process of how original data to be transformed, cleared, integrated, and demonstrated. This study adopted a medical university library and its subscription of medical electronic resources as a case. Our data analysis includes (1 year of subscription,(2 title of journal, (3 affiliation, (4 subjects, and (5 specific journal requirements, etc. The findings of the study are contributed to obtain further understanding in policy making and user behavior analysis. The integrated data provides multiple applications in informatics research, information behavior, bibliomining, presenting diverse views and extended issues for further discussion.

  16. An anti-tumor protein produced by Trichinella spiralis and identified by screening a T7 phage display library, induces apoptosis in human hepatoma H7402 cells

    Science.gov (United States)

    Trichinella spiralis infection confers effective resistance to tumor cell expansion. In this study, a T7 phage cDNA display library was constructed to express genes encoded by T. spiralis. Organic phase multi-cell screening was used to sort through candidate proteins in a transfected human chronic m...

  17. Homology modelling and bivalent single-chain Fv construction of anti-HepG2 single-chain immunoglobulin Fv fragments from a phage display library

    Indian Academy of Sciences (India)

    Ming Ni; Bing Yu; Y U Huang; Zhenjie Tang; Ping Lei; Xin Shen; Wei Xin; Huifen Zhu; Guanxin Shen

    2008-12-01

    We prepared single-chain immunoglobulin Fv fragments (scFv) SLH10 specific for the HepG2 cell line after biopanning from a large human-naïve phage display library (Griffin. 1 Library). The three-dimensional (3D) structure of SLH10 was modelled by the Insight II molecule simulation software. The structure was refined using the molecular dynamics method. The structures with the least steric clashes and lowest energy were determined finally. The optimized structures of heavy (VH) and light (VL) variable chains of SLH10 scFv were obtained. Then SLH10 bivalent single-chain Fv (BsFv) was constructed that would be suitable for high-affinity targeting. SLH10 BsFv was generated by linking scFvs together and identified by sequencing. Its expression products were confirmed by western blot analysis. The relative molecular masses of scFv and BsFv were approximately 30 kDa and 60 kDa, respectively. Flow cytometry revealed that SLH10 BsFv bound the selected cell lines with greater signal intensity than the parental scFv. The improved antigen binding of SLH10 BsFv may be useful for immunodiagnostics or targeted gene therapy for liver cancer.

  18. Updating the Cornell Net Carbohydrate and Protein System feed library and analyzing model sensitivity to feed inputs.

    Science.gov (United States)

    Higgs, R J; Chase, L E; Ross, D A; Van Amburgh, M E

    2015-09-01

    The Cornell Net Carbohydrate and Protein System (CNCPS) is a nutritional model that evaluates the environmental and nutritional resources available in an animal production system and enables the formulation of diets that closely match the predicted animal requirements. The model includes a library of approximately 800 different ingredients that provide the platform for describing the chemical composition of the diet to be formulated. Each feed in the feed library was evaluated against data from 2 commercial laboratories and updated when required to enable more precise predictions of dietary energy and protein supply. A multistep approach was developed to predict uncertain values using linear regression, matrix regression, and optimization. The approach provided an efficient and repeatable way of evaluating and refining the composition of a large number of different feeds against commercially generated data similar to that used by CNCPS users on a daily basis. The protein A fraction in the CNCPS, formerly classified as nonprotein nitrogen, was reclassified to ammonia for ease and availability of analysis and to provide a better prediction of the contribution of metabolizable protein from free AA and small peptides. Amino acid profiles were updated using contemporary data sets and now represent the profile of AA in the whole feed rather than the insoluble residue. Model sensitivity to variation in feed library inputs was investigated using Monte Carlo simulation. Results showed the prediction of metabolizable energy was most sensitive to variation in feed chemistry and fractionation, whereas predictions of metabolizable protein were most sensitive to variation in digestion rates. Regular laboratory analysis of samples taken on-farm remains the recommended approach to characterizing the chemical components of feeds in a ration. However, updates to the CNCPS feed library provide a database of ingredients that are consistent with current feed chemistry information and

  19. The Data Model Resource Book A Library of Universal Data Models for All Enterprises

    CERN Document Server

    Silverston, Len

    2008-01-01

    A quick and reliable way to build proven databases for core business functionsIndustry experts raved about The Data Model Resource Book when it was first published in March 1997 because it provided a simple, cost-effective way to design databases for core business functions. Len Silverston has now revised and updated the hugely successful First Edition, while adding a companion volume to take care of more specific requirements of different businesses. Each volume is accompanied by a CD-ROM, which is sold separately. Each CD-ROM provides powerful design templates discussed in the books in a rea

  20. PEM Fuel Cells - Fundamentals, Modeling and Applications

    Directory of Open Access Journals (Sweden)

    Maher A.R. Sadiq Al-Baghdadi

    2013-01-01

    Full Text Available Part I: Fundamentals Chapter 1: Introduction. Chapter 2: PEM fuel cell thermodynamics, electrochemistry, and performance. Chapter 3: PEM fuel cell components. Chapter 4: PEM fuel cell failure modes. Part II: Modeling and Simulation Chapter 5: PEM fuel cell models based on semi-empirical simulation. Chapter 6: PEM fuel cell models based on computational fluid dynamics. Part III: Applications Chapter 7: PEM fuel cell system design and applications.

  1. PEM Fuel Cells - Fundamentals, Modeling and Applications

    OpenAIRE

    Maher A.R. Sadiq Al-Baghdadi

    2013-01-01

    Part I: Fundamentals Chapter 1: Introduction. Chapter 2: PEM fuel cell thermodynamics, electrochemistry, and performance. Chapter 3: PEM fuel cell components. Chapter 4: PEM fuel cell failure modes. Part II: Modeling and Simulation Chapter 5: PEM fuel cell models based on semi-empirical simulation. Chapter 6: PEM fuel cell models based on computational fluid dynamics. Part III: Applications Chapter 7: PEM fuel cell system design and applications.

  2. Community Digital Library Requirements for the Southern California Earthquake Center Community Modeling Environment (SCEC/CME)

    Science.gov (United States)

    Moore, R.; Faerman, M.; Minster, J.; Day, S. M.; Ely, G.

    2003-12-01

    A community digital library provides support for ingestion, organization, description, preservation, and access of digital entities. The technologies that traditionally provide these capabilities are digital libraries (ingestion, organization, description), persistent archives (preservation) and data grids (access). We present a design for the SCEC community digital library that incorporates aspects of all three systems. Multiple groups have created integrated environments that sustain large-scale scientific data collections. By examining these projects, the following stages of implementation can be identified: \\begin{itemize} Definition of semantic terms to associate with relevant information. This includes definition of uniform content descriptors to describe physical quantities relevant to the scientific discipline, and creation of concept spaces to define how the uniform content descriptors are logically related. Organization of digital entities into logical collections that make it simple to browse and manage related material. Definition of services that are used to access and manipulate material in the collection. Creation of a preservation environment for the long-term management of the collection. Each community is faced with heterogeneity that is introduced when data is distributed across multiple sites, or when multiple sets of collection semantics are used, and or when multiple scientific sub-disciplines are federated. We will present the relevant standards that simplify the implementation of the SCEC community library, the resource requirements for different types of data sets that drive the implementation, and the digital library processes that the SCEC community library will support. The SCEC community library can be viewed as the set of processing steps that are required to build the appropriate SCEC reference data sets (SCEC approved encoding format, SCEC approved descriptive metadata, SCEC approved collection organization, and SCEC managed storage

  3. Library statistics.

    OpenAIRE

    Melita Ambrožič

    1991-01-01

    The contribution deals with the purpose, beginnings and development of library statistics and the strivings for international standardization in this field. International recommendations are presented, as well as the ISO standard for library statistics. An overview is given of the theoretical contributions and statistical practice in Slovenian librarianship. Cautionary notice on the limitations of the applicability of library statistics in determining library performance is given and the inte...

  4. Digital Libraries

    CERN Document Server

    Papy, Fabrice

    2008-01-01

    Of vital interest to all librarians and information specialists, this book presents all aspects of the effects of digitization of today's and tomorrow's libraries. From social to technical issues, Digital Libraries includes chapters on the growth of the role of librarian, the reader experience, cataloging, search engines, OPAC, law, ergonomic studies, and the future of libraries.

  5. Identification of Eusynstyelamide B as a Potent Cell Cycle Inhibitor Following the Generation and Screening of an Ascidian-Derived Extract Library Using a Real Time Cell Analyzer

    Directory of Open Access Journals (Sweden)

    Michelle S. Liberio

    2014-10-01

    Full Text Available Ascidians are marine invertebrates that have been a source of numerous cytotoxic compounds. Of the first six marine-derived drugs that made anticancer clinical trials, three originated from ascidian specimens. In order to identify new anti-neoplastic compounds, an ascidian extract library (143 samples was generated and screened in MDA-MB-231 breast cancer cells using a real-time cell analyzer (RTCA. This resulted in 143 time-dependent cell response profiles (TCRP, which are read-outs of changes to the growth rate, morphology, and adhesive characteristics of the cell culture. Twenty-one extracts affected the TCRP of MDA-MB-231 cells and were further investigated regarding toxicity and specificity, as well as their effects on cell morphology and cell cycle. The results of these studies were used to prioritize extracts for bioassay-guided fractionation, which led to the isolation of the previously identified marine natural product, eusynstyelamide B (1. This bis-indole alkaloid was shown to display an IC50 of 5 µM in MDA-MB-231 cells. Moreover, 1 caused a strong cell cycle arrest in G2/M and induced apoptosis after 72 h treatment, making this molecule an attractive candidate for further mechanism of action studies.

  6. Identification of cancer specific ligands from one-bead one compound combinatorial libraries to develop theranostics agents against oral squamous cell carcinoma

    Science.gov (United States)

    Yang, Frances Fan

    Background: Oral squamous cell carcinoma (OSCC) is one of the most prevalent disease worldwide. One-bead one-compound (OBOC) combinatorial technology is a powerful method to identify peptidomimetic ligands against a variety of receptors on cell surfaces. We therefore hypothesized that cancer specific ligands against OSCC might be identified and can be conjugated to optical dyes or nanocarriers to develop theranostic agents against OSCC. Material and methods: Different OSCC cell lines were incubated with OBOC libraries and beads with cell binding were sorted and then screened with normal human cells to identify peptide-beads binding to different OSCC cell lines but not binding to normal human cells. The molecular probes of OSCC were developed by biotinylating the carboxyl end of the ligands. OSCC theranostic agents were developed by decorating LLY13 with NPs and evaluated by using orthotopic bioluminescent oral cancer model. Results: Six OSCC specific ligands were discovered. Initial peptide-histochemistry study indicated that LLY12 and LLY13 were able to specifically detect OSCC cells grown on chamber slides at the concentration of 1 muM. In addition, LLY13 was found to penetrate into the OSCC cells and accumulate in the cytoplasm, and nucleus. After screened with a panel of integrin antibodies, only anti-alpha3 antibody was able to block most of OSCC cells binding to the LLY13 beads. OSCC theranostic agents developed using targeting LLY13 micelles (25+/- 4nm in diameter) were more efficient in binding to HSC-3 cancer cells compared to non-targeting micelles. Ex vivo images demonstrated that xenografts from the mice with targeting micelles appeared to have higher signals than the non-targeting groups. Conclusion: LLY13 has promising in vitro and in vivo targeting activity against OSCC. In addition, LLY13 is also able to penetrate into cancer cells via endocytosis. Initial study indicated that alpha3 integrin might partially be the corresponding receptor involved

  7. Applying DEA Technique to Library Evaluation in Academic Research Libraries.

    Science.gov (United States)

    Shim, Wonsik

    2003-01-01

    This study applied an analytical technique called Data Envelopment Analysis (DEA) to calculate the relative technical efficiency of 95 academic research libraries, all members of the Association of Research Libraries. DEA, with the proper model of library inputs and outputs, can reveal best practices in the peer groups, as well as the technical…

  8. A theoretical model to predict customer satisfaction in relation to service quality in selected university libraries in Sri Lanka

    Directory of Open Access Journals (Sweden)

    Chaminda Jayasundara

    2009-01-01

    Full Text Available University library administrators in Sri Lanka have begun to search for alternative ways to satisfy their clientele on the basis of service quality. This article aims at providing a theoretical model to facilitate the identification of service quality attributes and domains that may be used to predict customer satisfaction from a service quality perspective. The effectiveness of existing service quality models such as LibQUAL, SERVQUAL and SERVPREF have been questioned. In that regard, this study developed a theoretical model for academic libraries in Sri Lanka based on the disconfirmation and performance-only paradigms. These perspectives were considered by researchers to be the core mechanism to develop service quality/customer satisfaction models. The attributes and domain identification of service quality was carried out with a stratified sample of 263 participants selected from postgraduate and undergraduate students and academic staff members from the faculties of Arts in four universities in Sri Lanka. The study established that responsiveness, supportiveness, building environment, collection and access, furniture and facilities, technology, Web services and service delivery were quality domains which can be used to predict customer satisfaction. The theoretical model is unique in its domain structure compared to the existing models. The model needs to be statistically tested to make it valid and parsimonious.

  9. SERVQUAL MODEL IMPLEMENTATION FOR EVALUATING QUALITY SERVICE OF THE LIBRARY OF HIGHER EDUCATIONINSTITUTION IN BOTUCATU REGION

    Directory of Open Access Journals (Sweden)

    Daniel Marques

    2014-12-01

    Full Text Available Currently technology colleges are growing and gaining market share. So, it is of paramount importance a careful evaluation of the most important components in an institution of higher education, the library, which is fundamental for the development of future professionals. Therefore, the implementation of a tool to measure and compare results through user satisfaction in relation to services provided to a particular service is required. SERVQUAL is a measurement tool, used in this study, capable of measuring expectations and perceptions of customers regarding rendered services. This tool is based on the theory of 5GAP's evaluation. This paper aimed at evaluating the services provided by the library of a Higher Education institution in Botucatu region, which is considered by the students as the most critical service. The reason for conducting this study is to present to the Academic Board quantified results on the most critical services rendered by the library, which can be accomplished through a simple and easily managed tool which can classify the most critical services and, if possible, correct them. The proposed project achieved its objectives showing quantified data on the needs of corrective actions to the most critical problems at the library, which are presented in tables throughout this paper according to undergraduate opinions

  10. PlantGL: A Python-based geometric library for 3D plant modelling at different scales

    OpenAIRE

    Pradal, Christophe; Boudon, Frédéric; Nouguier, Christophe; Chopard, Jérôme; Godin, Christophe

    2009-01-01

    International audience In this paper, we present PlantGL, an open-source graphic toolkit for the creation, simulation and analysis of 3D virtual plants. This C++ geometric library is embedded in the Python language which makes it a powerful user-interactive platform for plant modeling in various biological application domains. PlantGL makes it possible to build and manipulate geometric models of plants or plant parts, ranging from tissues and organs to plant populations. Based on a scene g...

  11. Mobile library services best practices

    CERN Document Server

    Harmon, Charles

    2013-01-01

    Mobile Library Services provides 11 proven ways to reach out to mobile users and increase your library's relevance to their day-to-day lives. Librarians detail how they created mobile apps to how they went mobile on a shoestring budget. Written by public, academic, and special librarians, these 11 best practices offer models for libraries of every type and size.

  12. Identification of novel compounds inhibiting chikungunya virus-induced cell death by high throughput screening of a kinase inhibitor library.

    Directory of Open Access Journals (Sweden)

    Deu John M Cruz

    Full Text Available Chikungunya virus (CHIKV is a mosquito-borne arthrogenic alphavirus that causes acute febrile illness in humans accompanied by joint pains and in many cases, persistent arthralgia lasting weeks to years. The re-emergence of CHIKV has resulted in numerous outbreaks in the eastern hemisphere, and threatens to expand in the foreseeable future. Unfortunately, no effective treatment is currently available. The present study reports the use of resazurin in a cell-based high-throughput assay, and an image-based high-content assay to identify and characterize inhibitors of CHIKV-infection in vitro. CHIKV is a highly cytopathic virus that rapidly kills infected cells. Thus, cell viability of HuH-7 cells infected with CHIKV in the presence of compounds was determined by measuring metabolic reduction of resazurin to identify inhibitors of CHIKV-associated cell death. A kinase inhibitor library of 4,000 compounds was screened against CHIKV infection of HuH-7 cells using the resazurin reduction assay, and the cell toxicity was also measured in non-infected cells. Seventy-two compounds showing ≥50% inhibition property against CHIKV at 10 µM were selected as primary hits. Four compounds having a benzofuran core scaffold (CND0335, CND0364, CND0366 and CND0415, one pyrrolopyridine (CND0545 and one thiazol-carboxamide (CND3514 inhibited CHIKV-associated cell death in a dose-dependent manner, with EC50 values between 2.2 µM and 7.1 µM. Based on image analysis, these 6 hit compounds did not inhibit CHIKV replication in the host cell. However, CHIKV-infected cells manifested less prominent apoptotic blebs typical of CHIKV cytopathic effect compared with the control infection. Moreover, treatment with these compounds reduced viral titers in the medium of CHIKV-infected cells by up to 100-fold. In conclusion, this cell-based high-throughput screening assay using resazurin, combined with the image-based high content assay approach identified compounds against

  13. Construction of a hepatic stellate cells subtracted cDNA library of differentially expressed genes in normal mice and mice with Schistosomiasis japonica

    Institute of Scientific and Technical Information of China (English)

    Zheng Min; Wu Yi-jun; Cai Wei-min; Weng Hong-lei; Liu Rong-hua

    2005-01-01

    To construct a hepatic stellate cells (HSCs) subtracted cDNA library to find differentially expressed genes in normal mice and mice infected with Schistosomajaponicum (S. japonicum). Suppression subtractive hybridization (SSH) was used. The cDNA fragments of normal mouse were compared to those of schistosoma-infected mice to find differentially expressed genes.Then differentially expressed cDNA fragments were directly inserted into T/A cloning vector to set up the subtractive library.Amplification of the library was carried out with transformation of DH5α. The amplified library contained more than 400 positive bacterial clones, which were then hybridized with forward and backward subtracted probes for differential screening. One hundred positive bacterial clones were randomly selected for sequencing and BLAST analysis. Finally, virtual Northern Blot confirmed such differential expression. The subtracted cDNA library of differentially expressed genes of HSCs was constructed successfully,the library is efficient and lays foundation for screening and cloning new and specific genes of schistosomiasis.

  14. Reference Services in Australian Academic Libraries are Becoming More Multifaceted, A Review of: Burke, L. “Models of Reference Services in Australian Academic Libraries.” Journal of Librarianship and Information Science 40.4 (2008: 269‐86.

    Directory of Open Access Journals (Sweden)

    David Herron

    2009-09-01

    Full Text Available Objective – To investigate the current organizational models for reference work in Australian academic libraries, and how these reference services are staffed.Design – Mixed methods.Setting – Academic universities in Australia.Subjects – Forty Council of Australian University Librarians (CAUL member libraries.Methods – A literature study was undertaken to (1 find a definition of reference services and (2 explore the development of reference service models over time. Statistics from the CAUL member libraries were studied for trends in student population and number of academic and library staff. A web‐based survey, with questions based on the findings in the literature study, was then distributed to the 40 Australian university libraries in 2006. Respondents were asked when the library commenced different reference services in five areas: formats in which the library received and responded to reference queries, information literacy, subject specialization, liaison activities, and collection development. Respondents also answered questions about the organization of the reference department, including: whether they had a separate or integrated model; the size of the reference collections; if they had a librarian dedicated to supporting students studying in remote or distant mode; if the interlibrary loans department was part of the suite of reference services; and if they had a mission or statement of purpose for their reference services department.Main Results – Based on the literature study, the working definition of reference services (1 for the project was “all activities which assist in providing relevant and appropriate information services to patrons” (270, including:•All interactions with patrons to assist them in their searches for information in all media types.•All training by librarians of patrons to be able to access information for themselves.•Activities to help the library stay informed of relevant developments

  15. A model library for dynamic transport and fate of micropollutants in integrated urban wastewater and stormwater systems

    DEFF Research Database (Denmark)

    Vezzaro, Luca; Benedetti, Lorenzo; Gevaert, Veerle;

    2014-01-01

    the elaboration of pollution control strategies (including both source control and treatment options) at the small spatial scale of urban areas. Existing and well-established water quality models for the different parts of the IUWS (e.g. ASM models) are extended by adding MP fate processes. These are...... modelled by using substance inherent properties, following an approach commonly used in large-scale MP multimedia fate and transport models. The chosen level of complexity ensures a low data requirement and minimizes the need for field measurements. Next to a synthesis of model applications, a didactic...... example is presented to illustrate the potential of the use of the developed model library for developing, evaluating and comparing strategies for reduction of MP emissions from urban areas....

  16. Comparison of manufactured and modeled solar cell

    OpenAIRE

    Strachala, D.; Hylský, J.

    2015-01-01

    The aim of the work is to compare the model of monocrystalline silicon solar cell in PC1D with the real solar cell structure in terms of using a model in manufacture process. Real solar cell was firstly measured and analyzed to determine input parameters for a simulation and then realized in free available PC1D software. Degree of conformity of modeled and real solar cell was in the end established for basic prediction of solar cell parameters before manufacturing process.

  17. Cost Accounting and Analysis for University Libraries

    Science.gov (United States)

    Leimkuhler, Ferdinand F.; Cooper, Michael D.

    1971-01-01

    The approach to library planning studied in this paper is the use of accounting models to measure library costs and implement program budgets. A cost-flow model for a university library is developed and tested with historical data from the General Library at the University of California, Berkeley. (4 references) (Author)

  18. Modular SiGe 130 nm Cell Library for Extreme Environments Project

    Data.gov (United States)

    National Aeronautics and Space Administration — NASA space missions utilizing application-specific integrated circuits (ASICs) under extreme conditions have a critical need for high performance analog cell...

  19. Cell-Based Selection Expands the Utility of DNA-Encoded Small-Molecule Library Technology to Cell Surface Drug Targets: Identification of Novel Antagonists of the NK3 Tachykinin Receptor.

    Science.gov (United States)

    Wu, Zining; Graybill, Todd L; Zeng, Xin; Platchek, Michael; Zhang, Jean; Bodmer, Vera Q; Wisnoski, David D; Deng, Jianghe; Coppo, Frank T; Yao, Gang; Tamburino, Alex; Scavello, Genaro; Franklin, G Joseph; Mataruse, Sibongile; Bedard, Katie L; Ding, Yun; Chai, Jing; Summerfield, Jennifer; Centrella, Paolo A; Messer, Jeffrey A; Pope, Andrew J; Israel, David I

    2015-12-14

    DNA-encoded small-molecule library technology has recently emerged as a new paradigm for identifying ligands against drug targets. To date, this technology has been used with soluble protein targets that are produced and used in a purified state. Here, we describe a cell-based method for identifying small-molecule ligands from DNA-encoded libraries against integral membrane protein targets. We use this method to identify novel, potent, and specific inhibitors of NK3, a member of the tachykinin family of G-protein coupled receptors (GPCRs). The method is simple and broadly applicable to other GPCRs and integral membrane proteins. We have extended the application of DNA-encoded library technology to membrane-associated targets and demonstrate the feasibility of selecting DNA-tagged, small-molecule ligands from complex combinatorial libraries against targets in a heterogeneous milieu, such as the surface of a cell.

  20. E-learning model for Polish libraries – BIBWEB. Case study

    OpenAIRE

    Bednarek-Michalska, Bożena; Wołodko, Anna

    2007-01-01

    This article describes important role of e-learning in Poland and cover experiences in transferring and localising the course, know-how about how to find strategic partners and establish a sound infrastructure for e-learning and guidelines how to get and use feedback from the learners to continuously improve the quality of the course. The article describes the situation in Polish library world and the process of implementation and conducting the online course for librarians “bibweb”. It also ...

  1. SOSpin, a C++ library for Yukawa decomposition in $\\mathsf{SO}(2N)$ models

    CERN Document Server

    Cardoso, Nuno; Gonçalves, Nuno; Simoes, C

    2015-01-01

    We present in this paper the SOSpin library, which calculates an analytic decomposition of the Yukawa interactions invariant under any $\\mathsf{SO}(2N)$ group in terms of an $\\mathsf{SU}(N)$ basis. We make use of the oscillator expansion formalism, where the $\\mathsf{SO}(2N)$ spinor representations are expressed in terms of creation and annihilation operators of a Grassmann algebra. These noncommutative operators and their products are simulated in SOSpin through the implementation of doubly-linked-list data structures. These data structures were determinant to achieve a higher performance in the simplification of large products of creation and annihilation operators. We illustrate the use of our library with complete examples of how to decompose Yukawa terms invariant under $\\mathsf{SO}(2N)$ in terms of $\\mathsf{SU}(N)$ degrees of freedom for $N=2$ and $5$. We further demonstrate, with an example for $\\mathsf{SO}(4)$, that higher dimensional field-operator terms can also be processed with our library. Finall...

  2. Structural libraries of protein models for multiple species to understand evolution of the renin-angiotensin system.

    Science.gov (United States)

    Prokop, Jeremy W; Petri, Victoria; Shimoyama, Mary E; Watanabe, Ingrid K M; Casarini, Dulce E; Leeper, Thomas C; Bilinovich, Stephanie M; Jacob, Howard J; Santos, Robson A S; Martins, Almir S; Araujo, Fabiano C; Reis, Fernando M; Milsted, Amy

    2015-05-01

    The details of protein pathways at a structural level provides a bridge between genetics/molecular biology and physiology. The renin-angiotensin system is involved in many physiological pathways with informative structural details in multiple components. Few studies have been performed assessing structural knowledge across the system. This assessment allows use of bioinformatics tools to fill in missing structural voids. In this paper we detail known structures of the renin-angiotensin system and use computational approaches to estimate and model components that do not have their protein structures defined. With the subsequent large library of protein structures, we then created a species specific protein library for human, mouse, rat, bovine, zebrafish, and chicken for the system. The rat structural system allowed for rapid screening of genetic variants from 51 commonly used rat strains, identifying amino acid variants in angiotensinogen, ACE2, and AT1b that are in contact positions with other macromolecules. We believe the structural map will be of value for other researchers to understand their experimental data in the context of an environment for multiple proteins, providing pdb files of proteins for the renin-angiotensin system in six species. With detailed structural descriptions of each protein, it is easier to assess a species for use in translating human diseases with animal models. Additionally, as whole genome sequencing continues to decrease in cost, tools such as molecular modeling will gain use as an initial step in designing efficient hypothesis driven research, addressing potential functional outcomes of genetic variants with precompiled protein libraries aiding in rapid characterizations.

  3. Cost Models in Digital Archiving: An overview of Life Cycle Management at the National Library of the Netherlands

    Directory of Open Access Journals (Sweden)

    Erik Oltmans

    2004-10-01

    Full Text Available The number of institutions that are either considering the implementation of a digital archive or already started with an operational digital archive service is increasing. While technological and organisational challenges are well-studied and in few cases even well-defined, the subject of costs often remains untouched, which in many cases prevent organisations from new initiatives. In this paper two leading digital preservation techniques - migration and emulation - will be discussed in terms of life cycle management and associated cost models. Both techniques are studied and considered for implementation at the Koninklijke Bibliotheek, National Library of the Netherlands.

  4. Recombinant human antibodies: linkage of an Fab fragment from a combinatorial library to an Fc fragment for expression in mammalian cell culture.

    Science.gov (United States)

    Bender, E; Woof, J M; Atkin, J D; Barker, M D; Bebbington, C R; Burton, D R

    1993-04-01

    The combinatorial phage library approach to immunoglobulin repertoire cloning recently made it possible to isolate gene fragments encoding human immunoglobulin G1 Fabs binding with high affinity to specific antigens. Here we describe the construction of genes encoding whole human anti-tetanus toxoid antibodies based on one of these gene fragments and the efficient expression of these constructs by co-transfection of separate heavy and light chain vectors into a Chinese hamster ovary cell line constitutively expressing a viral transactivator protein. This system will be generally useful for the rapid analysis of recombinant antibodies derived from combinatorial libraries. PMID:8518367

  5. Library Locations

    Data.gov (United States)

    Allegheny County / City of Pittsburgh / Western PA Regional Data Center — Carnegie Library of Pittsburgh locations including address, coordinates, phone number, square footage, and standard operating hours.

  6. In situ isolation of mRNA from individual plant cells: creation of cell-specific cDNA libraries

    NARCIS (Netherlands)

    Karrer, E.E.; Lincoln, J.E.; Hogenhout, S.A.; Bennett, A.B.; Bostock, R.M.; Martineau, B.; Lucas, W.J.; Gilchrist, D.G.; Alexander, D.

    1995-01-01

    A method for isolating and cloning mRNA populations from individual cells in living, intact plant tissues is described. The contents of individual cells were aspirated into micropipette tips filled with RNA extraction buffer. The mRNA from these cells was purified by binding to oligo(dT)-linked magn

  7. America's Star Libraries: Top-Rated Libraries

    Science.gov (United States)

    Lance, Keith Curry; Lyons, Ray

    2009-01-01

    "Library Journal"'s national rating of public libraries, the "LJ" Index of Public Library Service 2009, Round 2, identifies 258 "star" libraries. Created by Keith Curry Lance and Ray Lyons and based on 2007 data from the IMLS, it rates 7,268 public libraries. The top libraries in each group get five, four, or three stars. All included libraries,…

  8. 图书馆精神的救赎——榜样教育%Salvation of Library Spirit --Model Education

    Institute of Scientific and Technical Information of China (English)

    陈文革

    2011-01-01

    The reason of library job burnout is the intelligence materialization. Library job burnout embodys development economism, social values orientated at money, cognitive bias of librarian values and library engaged in business. Library job burnout endangers the development of librarians and libraries, its substance is lack of library spirit. Model education could nurture library spirit effectively and overcome job burnout, so as to promote the development of librarianship.%图书馆职业懈怠产生的原因在于智力物化,具体表现为发展唯经济论、社会价值取向金钱化、图书馆员价值认知偏差、图书馆经营化4个方面。图书馆职业懈怠危害了馆员个人和图书馆事业的发展,其实质是图书馆精神缺失。开展图书馆榜样教育可以有效培育图书馆精神,克服职业懈怠,从而推动图书馆事业发展。

  9. An expressed sequence tag (EST library for Drosophila serrata, a model system for sexual selection and climatic adaptation studies

    Directory of Open Access Journals (Sweden)

    McGraw Elizabeth A

    2009-01-01

    Full Text Available Abstract Background The native Australian fly Drosophila serrata belongs to the highly speciose montium subgroup of the melanogaster species group. It has recently emerged as an excellent model system with which to address a number of important questions, including the evolution of traits under sexual selection and traits involved in climatic adaptation along latitudinal gradients. Understanding the molecular genetic basis of such traits has been limited by a lack of genomic resources for this species. Here, we present the first expressed sequence tag (EST collection for D. serrata that will enable the identification of genes underlying sexually-selected phenotypes and physiological responses to environmental change and may help resolve controversial phylogenetic relationships within the montium subgroup. Results A normalized cDNA library was constructed from whole fly bodies at several developmental stages, including larvae and adults. Assembly of 11,616 clones sequenced from the 3' end allowed us to identify 6,607 unique contigs, of which at least 90% encoded peptides. Partial transcripts were discovered from a variety of genes of evolutionary interest by BLASTing contigs against the 12 Drosophila genomes currently sequenced. By incorporating into the cDNA library multiple individuals from populations spanning a large portion of the geographical range of D. serrata, we were able to identify 11,057 putative single nucleotide polymorphisms (SNPs, with 278 different contigs having at least one "double hit" SNP that is highly likely to be a real polymorphism. At least 394 EST-associated microsatellite markers, representing 355 different contigs, were also found, providing an additional set of genetic markers. The assembled EST library is available online at http://www.chenowethlab.org/serrata/index.cgi. Conclusion We have provided the first gene collection and largest set of polymorphic genetic markers, to date, for the fly D. serrata. The EST

  10. Cellular cardiac electrophysiology modelling with Chaste and CellML

    Directory of Open Access Journals (Sweden)

    Jonathan eCooper

    2015-01-01

    Full Text Available Chaste is an open-source C++ library for computational biology that has well-developed cardiac electrophysiology tissue simulation support. In this paper, we introduce the features available for performing cardiac electrophysiology action potential simulations using a wide range of models from the Physiome repository.The mathematics of the models are described in CellML, with units for all quantities. The primary idea is that the model is defined in one place (the CellML file, and all model code is auto-generated at compile or run time; it never has to be manually edited.We use ontological annotation to identify model variables describing certain biological quantities (membrane voltage, capacitance, etc. to allow us to import any relevant CellML models into the Chaste framework in consistent units, and to interact with them via consistent interfaces. This approach provides a great deal of flexibility for analysing different models of the same system. Chaste provides a wide choice of numerical methods for solving the ordinary differential equations that describe the models. Fixed-timestep explicit and implicit solvers are provided, as discussed in previous work. Here we introduce the Rush--Larsen and Generalised Rush--Larsen integration techniques, made available via symbolic manipulation of the model equations, which are automatically rearranged into the forms required by these approaches. We have also integrated the CVODE solvers, a `gold standard' for stiff systems, and we have developed support for symbolic computation of the Jacobian matrix, yielding further increases in the performance and accuracy of CVODE. We discuss some of the technical details of this work and compare the performance of the available numerical methods.Finally, we discuss how this is generalised in our functional curation framework, which uses a domain-specific language for defining complex experiments as a basis for comparison of model behaviour.

  11. The quality maturity model: assessing organisational quality culture in academic libraries

    OpenAIRE

    Wilson, Frances

    2013-01-01

    This thesis was submitted for the degree of Doctor of Philosophy and awarded by Brunel University Academic libraries operate in a fluid environment, where they must provide, and demonstrate that they provide, a high quality service that is focussed on customers’ needs. It is broadly accepted that the way to provide a high quality service responsive to customers’ needs is to have a culture of quality that underpins all the organisation’s efforts, i.e. TQM. The literature on how to improve t...

  12. Modeling Rett Syndrome with Stem Cells

    OpenAIRE

    Walsh, Ryan M.; Hochedlinger, Konrad

    2010-01-01

    The discovery that somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) raised the exciting possibility of modeling diseases with patient-specific cells. Marchetto et al. (2010) now use iPSC technology to generate, characterize, and treat an in vitro model for the autism spectrum disorder, Rett syndrome.

  13. Libraries - Iowa DNR - NRGIS Library

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — The Natural Resources Geographic Information System (NRGIS) Library is a Geographic Information System (GIS) repository developed and maintained by the GIS Section...

  14. Learning Commons in Academic Libraries

    Directory of Open Access Journals (Sweden)

    Larisa González Martínez

    2015-01-01

    Full Text Available Like all human creations, institutions transform and evolve over time. Libraries also have changed to respond the needs of its users. Academic libraries physical spaces are one of the turned aspects, an example are the Learning Commons (spaces for collaborative work in academic libraries. The main purpose of this paper is to expose the characteristics of the Learning Commons model with a brief account of the history of planning and construction of academic libraries. This paper also aims to present the manner in which a Learning Commons has been implemented at the library of Instituto Tecnológico y de Estudios Superiores de Monterrey (ITESM, Campus Monterrey in Mexico.

  15. Privatizing Libraries

    Science.gov (United States)

    Jerrard, Jane; Bolt, Nancy; Strege, Karen

    2012-01-01

    This timely special report from ALA Editions provides a succinct but comprehensive overview of the "privatization" of public libraries. It provides a history of the trend of local and state governments privatizing public services and assets, and then examines the history of public library privatization right up to the California legislation…

  16. Scanning electron microscope measurement of width and shape of 10 nm patterned lines using a JMONSEL-modeled library

    Energy Technology Data Exchange (ETDEWEB)

    Villarrubia, J.S., E-mail: john.villarrubia@nist.gov [Semiconductor and Dimensional Metrology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 (United States); Vladár, A.E.; Ming, B. [Semiconductor and Dimensional Metrology Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 (United States); Kline, R.J.; Sunday, D.F. [Materials Science and Engineering Division, National Institute of Standards and Technology, Gaithersburg, MD 20899 (United States); Chawla, J.S.; List, S. [Intel Corporation, RA3-252, 5200 NE Elam Young Pkwy, Hillsboro, OR 97124 (United States)

    2015-07-15

    The width and shape of 10 nm to 12 nm wide lithographically patterned SiO{sub 2} lines were measured in the scanning electron microscope by fitting the measured intensity vs. position to a physics-based model in which the lines' widths and shapes are parameters. The approximately 32 nm pitch sample was patterned at Intel using a state-of-the-art pitch quartering process. Their narrow widths and asymmetrical shapes are representative of near-future generation transistor gates. These pose a challenge: the narrowness because electrons landing near one edge may scatter out of the other, so that the intensity profile at each edge becomes width-dependent, and the asymmetry because the shape requires more parameters to describe and measure. Modeling was performed by JMONSEL (Java Monte Carlo Simulation of Secondary Electrons), which produces a predicted yield vs. position for a given sample shape and composition. The simulator produces a library of predicted profiles for varying sample geometry. Shape parameter values are adjusted until interpolation of the library with those values best matches the measured image. Profiles thereby determined agreed with those determined by transmission electron microscopy and critical dimension small-angle x-ray scattering to better than 1 nm.

  17. Scanning electron microscope measurement of width and shape of 10 nm patterned lines using a JMONSEL-modeled library

    International Nuclear Information System (INIS)

    The width and shape of 10 nm to 12 nm wide lithographically patterned SiO2 lines were measured in the scanning electron microscope by fitting the measured intensity vs. position to a physics-based model in which the lines' widths and shapes are parameters. The approximately 32 nm pitch sample was patterned at Intel using a state-of-the-art pitch quartering process. Their narrow widths and asymmetrical shapes are representative of near-future generation transistor gates. These pose a challenge: the narrowness because electrons landing near one edge may scatter out of the other, so that the intensity profile at each edge becomes width-dependent, and the asymmetry because the shape requires more parameters to describe and measure. Modeling was performed by JMONSEL (Java Monte Carlo Simulation of Secondary Electrons), which produces a predicted yield vs. position for a given sample shape and composition. The simulator produces a library of predicted profiles for varying sample geometry. Shape parameter values are adjusted until interpolation of the library with those values best matches the measured image. Profiles thereby determined agreed with those determined by transmission electron microscopy and critical dimension small-angle x-ray scattering to better than 1 nm

  18. 图书馆知识共享空间模型构建研究%Research on a Model of Library Knowledge Commons

    Institute of Scientific and Technical Information of China (English)

    朱红涛; 周九常; 胡守敏

    2011-01-01

    图书馆知识共享空间是满足用户知识共享活动服务需求的重要平台和场所。文章在界定图书馆知识共享空间概念的基础上,分析图书馆知识共享空间运行需求,构建出包括知识共享平台、知识资源供给等模块的图书馆知识共享空间模型。%Library knowledge commons is an important platform and location by which libraries provide services for users' knowledge sharing.Based on defining the conception of library knowledge commons,the authors analyze its operating needs,and then construct a model of library knowledge commons,which includes knowledge sharing platform,knowledge resources supply,and other modules.

  19. Grading Model of College Library Crisis Management%高校图书馆危机管理之分级模式

    Institute of Scientific and Technical Information of China (English)

    裴允; 陈颖颖; 郭楠; 赵婷

    2011-01-01

    高校图书馆危机管理的能力与成效直接关系到图书馆服务的质量及声誉,甚至关系到学校的整体形象.本文根据危机的强度与频度对高校图书馆的危机进行分级,并制定相应的预警和对策,为高校图书馆危机管理提供了一个全新的管理模式.%The ability and effectiveness of university library crisis management are directly related to library services quality and reputation, even to the school's overall image.Based on the intensity and frequency of the crisis, the university library crises are graded, and appropriate early warning and response are made, providing a new management model for crisis management of university library.

  20. Novel 3D modeling methods for virtual fabrication and EDA compatible design of MEMS via parametric libraries

    Science.gov (United States)

    Schröpfer, Gerold; Lorenz, Gunar; Rouvillois, Stéphane; Breit, Stephen

    2010-06-01

    This paper provides a brief summary of the state-of-the-art of MEMS-specific modeling techniques and describes the validation of new models for a parametric component library. Two recently developed 3D modeling tools are described in more detail. The first one captures a methodology for designing MEMS devices and simulating them together with integrated electronics within a standard electronic design automation (EDA) environment. The MEMS designer can construct the MEMS model directly in a 3D view. The resulting 3D model differs from a typical feature-based 3D CAD modeling tool in that there is an underlying behavioral model and parametric layout associated with each MEMS component. The model of the complete MEMS device that is shared with the standard EDA environment can be fully parameterized with respect to manufacturing- and design-dependent variables. Another recent innovation is a process modeling tool that allows accurate and highly realistic visualization of the step-by-step creation of 3D micro-fabricated devices. The novelty of the tool lies in its use of voxels (3D pixels) rather than conventional 3D CAD techniques to represent the 3D geometry. Case studies for experimental devices are presented showing how the examination of these virtual prototypes can reveal design errors before mask tape out, support process development before actual fabrication and also enable failure analysis after manufacturing.

  1. Novel 3D modeling methods for virtual fabrication and EDA compatible design of MEMS via parametric libraries

    International Nuclear Information System (INIS)

    This paper provides a brief summary of the state-of-the-art of MEMS-specific modeling techniques and describes the validation of new models for a parametric component library. Two recently developed 3D modeling tools are described in more detail. The first one captures a methodology for designing MEMS devices and simulating them together with integrated electronics within a standard electronic design automation (EDA) environment. The MEMS designer can construct the MEMS model directly in a 3D view. The resulting 3D model differs from a typical feature-based 3D CAD modeling tool in that there is an underlying behavioral model and parametric layout associated with each MEMS component. The model of the complete MEMS device that is shared with the standard EDA environment can be fully parameterized with respect to manufacturing- and design-dependent variables. Another recent innovation is a process modeling tool that allows accurate and highly realistic visualization of the step-by-step creation of 3D micro-fabricated devices. The novelty of the tool lies in its use of voxels (3D pixels) rather than conventional 3D CAD techniques to represent the 3D geometry. Case studies for experimental devices are presented showing how the examination of these virtual prototypes can reveal design errors before mask tape out, support process development before actual fabrication and also enable failure analysis after manufacturing.

  2. Attitudes of University Librarians and Information Scientists towards the Draft Code of Library Ethics to Present a Model for Final Library Ethical Codes

    OpenAIRE

    Latifi, Mahsoomeh; Zandian, Fatemeh; Siamian, Hasan

    2011-01-01

    This research performed to survey attitudes of Iranian Academic librarians and faculty members towards the draft code of library professional ethics for Iran. The researcher used the survey research methodology for this study. The researchers used two questionnaires for the analysis in this study. The researcher collected, analyzed, and reported data for a population of university librarians 445 in 33 governmental universities in seven provinces and 62 faculty membe...

  3. Cell culture models using rat primary alveolar type I cells.

    Science.gov (United States)

    Downs, Charles A; Montgomery, David W; Merkle, Carrie J

    2011-10-01

    There is a lack of cell culture models using primary alveolar type I (AT I) cells. The purpose of this study was to develop cell culture models using rat AT I cells and microvascular endothelial cells from the lung (MVECL). Two types of model systems were developed: single and co-culture systems; additionally a 3-dimensional model system was developed. Pure AT I cell (96.3 ± 2.7%) and MVECL (97.9 ± 1.1%) preparations were used. AT I cell morphology, mitochondrial number and distribution, actin filament arrangement and number of apoptotic cells at confluence, and telomere attrition were characterized. AT I cells maintained their morphometric characteristics through at least population doubling (PD) 35, while demonstrating telomere attrition through at least PD 100. Furthermore, AT I cells maintained the expression of their specific markers, T1α and AQ-5, through PD 42. For the co-cultures, AT I cells were grown on the top and MVECL were grown on the bottom of fibronectin-coated 24-well Transwell Fluroblok™ filter inserts. Neither cell type transmigrated the 1 μm pores. Additionally, AT I cells were grown in a thick layer of Matrigel(®) to create a 3-dimensional model in which primary AT I cells form ring-like structures that resemble an alveolus. The development of these model systems offers the opportunities to investigate AT I cells and their interactions with MVECL in response to pharmacological interventions and in the processes of disease, repair and regeneration. PMID:21624488

  4. Cell Division in the Light of Modeling.

    Science.gov (United States)

    Bellaïche, Yohanns

    2016-09-26

    Theoretical modeling is central to elucidating underlying principles of emergent properties of complex systems. In cell and developmental biology, the last 15 years have witnessed a convergence of empirical and modeling approaches for fresh perspectives. The role of cell division in coordinating size, shape, and fate in particular illustrates the ever-growing impact of modeling.

  5. Including excitons in semiconductor solar cell modelling

    OpenAIRE

    Burgelman, Marc; Minnaert, Ben

    2005-01-01

    Excitons are marginally important in classical semiconductor device physics, and their treatment is not included in standard solar cell modelling. However, in organic semiconductors and solar cells, the role of excitons is essential, as the primary effect of light absorption is exciton generation, and free electrons and holes are created by exciton dissociation. First steps to include excitons in solar cell modelling were presented by Green 1996 and Zhang 1998. Their model was restricted to a...

  6. Cooperation Model and Future Development Between NGO and Libraries%NGO与图书馆的合作模式及发展方向

    Institute of Scientific and Technical Information of China (English)

    张治理

    2011-01-01

    This paper learns successful experience from NGO of developed countries, analyzes NGO' s role and issues in the process of Chinese library development, including cooperation models, NGO' s impact on library service and construction as well as some cooperation issues needed to be solved between NGO and library. On the basis of that, it proposes that the Library Society of China should play an important role in bringing NGO into library community to advance the development of Chinese libraries.%借鉴发达国家NGO的成功经验,分析NGO在中国图书馆发展过程中的状况和问题,包括NGO与图书馆的合作形式、NGO对图书馆建设与服务的影响及NGO与图书馆合作中一些亟待解决的问题。在此基础上,提出问题的解决方法:充分发挥中国图书馆学会的作用,将NGO有效地引入到图书馆事业中来,从而推动中国图书馆事业的发展。

  7. Research on Library E-book Service Model%图书馆电子书服务模式探微

    Institute of Scientific and Technical Information of China (English)

    吴起立

    2015-01-01

    电子书服务拓展了图书馆服务的广度,对人们的阅读将产生深远的影响。为此,结合国内外电子书研究现状分析,详细地探讨了广告植入服务、图书馆联盟服务、采访服务、借阅服务、出版商图书馆联盟服务等面向图书馆的电子书服务模式,提出从5个方面着手以应用与推广面向图书馆电子书服务模式,将为图书馆服务创新提供一个新方法、新思维。%E-Book services expand the library services for the people’s reading, which wil have a profound impact. Combining the analysis of the current status of e-books, this paper discusses in detail library oriented e-book services, such as placement services, library al iance services, interviews services, loan services, publishers-library al iance services, and it proceed with the application and promotion of e-books for library service model from five aspects, which wil provide a new approach, new thinking library service innovation.

  8. NSUF Irradiated Materials Library

    Energy Technology Data Exchange (ETDEWEB)

    Cole, James Irvin [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-09-01

    The Nuclear Science User Facilities has been in the process of establishing an innovative Irradiated Materials Library concept for maximizing the value of previous and on-going materials and nuclear fuels irradiation test campaigns, including utilization of real-world components retrieved from current and decommissioned reactors. When the ATR national scientific user facility was established in 2007 one of the goals of the program was to establish a library of irradiated samples for users to access and conduct research through competitively reviewed proposal process. As part of the initial effort, staff at the user facility identified legacy materials from previous programs that are still being stored in laboratories and hot-cell facilities at the INL. In addition other materials of interest were identified that are being stored outside the INL that the current owners have volunteered to enter into the library. Finally, over the course of the last several years, the ATR NSUF has irradiated more than 3500 specimens as part of NSUF competitively awarded research projects. The Logistics of managing this large inventory of highly radioactive poses unique challenges. This document will describe materials in the library, outline the policy for accessing these materials and put forth a strategy for making new additions to the library as well as establishing guidelines for minimum pedigree needed to be included in the library to limit the amount of material stored indefinitely without identified value.

  9. Keratinocyte growth factor phage model peptides can promote epidermal cell proliferation without tumorigenic effect

    Institute of Scientific and Technical Information of China (English)

    ZONG Xian-lei; JIANG Du-yin; WANG Ji-chang; LIU Jun-li; LIU Zhen-zhong; CAI Jing-long

    2010-01-01

    Background Keratinocyte growth factor (KGF) significantly influences epithelial wound healing. The aim of this study was to isolate KGF phage model peptides from a phage display 7-mer peptide library to evaluate their effect on promoting epidermal cell proliferation. Methods A phage display 7-mer peptide library was screened using monoclonal anti-human KGF antibody as the target. Enzyme linked immunosorbent assay (ELISA) was performed to select monoclonal phages with good binding activity. DNA sequencing was done to find the similarities of model peptides. Three-(4,5-dimethylthiazol-2-yl) -2,5-diphenyl tetrazolium bromide (MTT) assay, immunofluorescence assay and quantitative real-time PCR analysis were employed to evaluate the effect of the phage model peptides on epidermal cells. Results Thirty-three out of fifty-eight (56.9%) of the isolated monoclonal phages exhibited high binding activity by ELISA. Ten of fifteen obtained phage model peptides were similar to KGF or epidermal growth factor (EGF). MTT assay data showed that four (No. 1-4) of the ten phage model peptides could promote epidermal cell proliferation. The expression of keratinocyte growth factor receptor (KGFR) mRNA in the KGF control group and the two phage model peptide groups (No. 1 and No. 2) increased. Expression of c-Fos mRNA and c-Jun mRNA in the KGF control group increased, but did not increase in the four phage model peptide groups (No.1-4). Conclusion Four phage model peptides isolated from the phage display 7-mer peptide library can safely promote epidermal cell proliferation without tumorigenic effect.

  10. Cloud Computing and Your Library

    Science.gov (United States)

    Mitchell, Erik T.

    2010-01-01

    One of the first big shifts in how libraries manage resources was the move from print-journal purchasing models to database-subscription and electronic-journal purchasing models. Libraries found that this transition helped them scale their resources and provide better service just by thinking a bit differently about their services. Likewise,…

  11. Screening and identification of a novel target specific for hepatoma cell line HepG2 from the FliTrx bacterial peptide library

    Institute of Scientific and Technical Information of China (English)

    Wenhan Li; Ping Lei; Bing Yu; Sha Wu; Jilin Peng; Xiaoping Zhao; Huffen Zhu; Michael Kirschfink; Guanxin Shen

    2008-01-01

    To explore new targets for hepatoma research, we used a surface display library to screen novel tumor cell-specific peptides. The bacterial FliTrx system was screened with living normal liver cell line L02 and hepatoma cell line HepG2 successively to search for hepatoma-specific peptides. Three clones (Hep1, Hep2, and Hep3) were identified to be specific to HepG2 compared with L02 and other cancer cell lines.Three-dimensional structural prediction proved that peptides inserted into the active site of Escherichia coli thioredoxin (TrxA) formed certain loop structures protruding out of the surface. Western blot analysis showed that FliC/TrxA-pepfide fusion proteins could be directly used to detect HepG2 cells.Three different FliC/TrxA-peptide fusion proteins targeted the same molecule, at approximately 140 kDa, on HepG2 cells.This work presented for the first time the application of the FliTrx library in screening living cells. Three peptides were obtained that could be potential candidates for targeted liver cancer therapy.

  12. Stochastic Gompertz model of tumour cell growth.

    Science.gov (United States)

    Lo, C F

    2007-09-21

    In this communication, based upon the deterministic Gompertz law of cell growth, a stochastic model in tumour growth is proposed. This model takes account of both cell fission and mortality too. The corresponding density function of the size of the tumour cells obeys a functional Fokker--Planck equation which can be solved analytically. It is found that the density function exhibits an interesting "multi-peak" structure generated by cell fission as time evolves. Within this framework the action of therapy is also examined by simply incorporating a therapy term into the deterministic cell growth term.

  13. Active Gel Model of Amoeboid Cell Motility

    CERN Document Server

    Callan-Jones, A C

    2013-01-01

    We develop a model of amoeboid cell motility based on active gel theory. Modeling the motile apparatus of a eukaryotic cell as a confined layer of finite length of poroelastic active gel permeated by a solvent, we first show that, due to active stress and gel turnover, an initially static and homogeneous layer can undergo a contractile-type instability to a polarized moving state in which the rear is enriched in gel polymer. This agrees qualitatively with motile cells containing an actomyosin-rich uropod at their rear. We find that the gel layer settles into a steadily moving, inhomogeneous state at long times, sustained by a balance between contractility and filament turnover. In addition, our model predicts an optimal value of the gel-susbstrate adhesion leading to maximum layer speed, in agreement with cell motility assays. The model may be relevant to motility of cells translocating in complex, confining environments that can be mimicked experimentally by cell migration through microchannels.

  14. Mathematical model of electrotaxis in osteoblastic cells

    NARCIS (Netherlands)

    Vanegas-Acosta, J.C.; Garzón-Alvarado, D.A.; Zwamborn, A.P.M.

    2012-01-01

    Electrotaxis is the cell migration in the presence of an electric field (EF). This migration is parallel to the EF vector and overrides chemical migration cues. In this paper we introduce a mathematical model for the electrotaxis in osteoblastic cells. The model is evaluated using different EF stren

  15. 高校图书馆学科服务模式探讨%Discussion on Academic Library Subject Service Model

    Institute of Scientific and Technical Information of China (English)

    潘可

    2012-01-01

    Subject service is an advanced concept to run a library and a new service model for international library community.With the development of user information needs and inforamtion environment as well as the requirements of self-development for libraries,subject service is a long-term trend for academic libraries.Through the research on current subject services implemented in Hubei academic libraries,the thesis indicates that establishing a subject team is a suitable subject service model for our academic libraies.%学科服务是国际图书馆界的一种先进的办馆理念和全新服务模式。随着用户信息需求、信息环境的变化以及图书馆自身发展的要求,图书馆实行学科服务是一个长期的趋势。通过湖北省高校图书馆学科服务的调研分析,认为学科团队是适合我国高校图书馆发展现状的学科服务模式。

  16. Modelling the exposure to chemicals for risk assessment: a comprehensive library of multimedia and PBPK models for integration, prediction, uncertainty and sensitivity analysis - the MERLIN-Expo tool.

    Science.gov (United States)

    Ciffroy, P; Alfonso, B; Altenpohl, A; Banjac, Z; Bierkens, J; Brochot, C; Critto, A; De Wilde, T; Fait, G; Fierens, T; Garratt, J; Giubilato, E; Grange, E; Johansson, E; Radomyski, A; Reschwann, K; Suciu, N; Tanaka, T; Tediosi, A; Van Holderbeke, M; Verdonck, F

    2016-10-15

    MERLIN-Expo is a library of models that was developed in the frame of the FP7 EU project 4FUN in order to provide an integrated assessment tool for state-of-the-art exposure assessment for environment, biota and humans, allowing the detection of scientific uncertainties at each step of the exposure process. This paper describes the main features of the MERLIN-Expo tool. The main challenges in exposure modelling that MERLIN-Expo has tackled are: (i) the integration of multimedia (MM) models simulating the fate of chemicals in environmental media, and of physiologically based pharmacokinetic (PBPK) models simulating the fate of chemicals in human body. MERLIN-Expo thus allows the determination of internal effective chemical concentrations; (ii) the incorporation of a set of functionalities for uncertainty/sensitivity analysis, from screening to variance-based approaches. The availability of such tools for uncertainty and sensitivity analysis aimed to facilitate the incorporation of such issues in future decision making; (iii) the integration of human and wildlife biota targets with common fate modelling in the environment. MERLIN-Expo is composed of a library of fate models dedicated to non biological receptor media (surface waters, soils, outdoor air), biological media of concern for humans (several cultivated crops, mammals, milk, fish), as well as wildlife biota (primary producers in rivers, invertebrates, fish) and humans. These models can be linked together to create flexible scenarios relevant for both human and wildlife biota exposure. Standardized documentation for each model and training material were prepared to support an accurate use of the tool by end-users. One of the objectives of the 4FUN project was also to increase the confidence in the applicability of the MERLIN-Expo tool through targeted realistic case studies. In particular, we aimed at demonstrating the feasibility of building complex realistic exposure scenarios and the accuracy of the

  17. Cytoview: Development of a cell modelling framework

    Indian Academy of Sciences (India)

    Prashant Khodade; Samta Malhotra; Nirmal Kumar; M Sriram Iyengar; N Balakrishnan; Nagasuma Chandra

    2007-08-01

    The biological cell, a natural self-contained unit of prime biological importance, is an enormously complex machine that can be understood at many levels. A higher-level perspective of the entire cell requires integration of various features into coherent, biologically meaningful descriptions. There are some efforts to model cells based on their genome, proteome or metabolome descriptions. However, there are no established methods as yet to describe cell morphologies, capture similarities and differences between different cells or between healthy and disease states. Here we report a framework to model various aspects of a cell and integrate knowledge encoded at different levels of abstraction, with cell morphologies at one end to atomic structures at the other. The different issues that have been addressed are ontologies, feature description and model building. The framework describes dotted representations and tree data structures to integrate diverse pieces of data and parametric models enabling size, shape and location descriptions. The framework serves as a first step in integrating different levels of data available for a biological cell and has the potential to lead to development of computational models in our pursuit to model cell structure and function, from which several applications can flow out.

  18. Minimal model for stem-cell differentiation

    Science.gov (United States)

    Goto, Yusuke; Kaneko, Kunihiko

    2013-09-01

    To explain the differentiation of stem cells in terms of dynamical systems theory, models of interacting cells with intracellular protein expression dynamics are analyzed and simulated. Simulations were carried out for all possible protein expression networks consisting of two genes under cell-cell interactions mediated by the diffusion of a protein. Networks that show cell differentiation are extracted and two forms of symmetric differentiation based on Turing's mechanism and asymmetric differentiation are identified. In the latter network, the intracellular protein levels show oscillatory dynamics at a single-cell level, while cell-to-cell synchronicity of the oscillation is lost with an increase in the number of cells. Differentiation to a fixed-point-type behavior follows with a further increase in the number of cells. The cell type with oscillatory dynamics corresponds to a stem cell that can both proliferate and differentiate, while the latter fixed-point type only proliferates. This differentiation is analyzed as a saddle-node bifurcation on an invariant circle, while the number ratio of each cell type is shown to be robust against perturbations due to self-consistent determination of the effective bifurcation parameter as a result of the cell-cell interaction. Complex cell differentiation is designed by combing these simple two-gene networks. The generality of the present differentiation mechanism, as well as its biological relevance, is discussed.

  19. E-Global Library: The Academic Campus Library Meets the Internet.

    Science.gov (United States)

    Heilig, Jean M.

    2001-01-01

    Describes e-global library, the first Internet-based virtual library designed for online students at Jones International University and that has grown into a separately licensable product. Highlights include marketing to other academic libraries, both online and traditional; fees; the e-global library model; collection development policies;…

  20. Malaria modeling: In vitro stem cells vs in vivo models.

    Science.gov (United States)

    Noulin, Florian

    2016-03-26

    The recent development of stem cell research and the possibility of generating cells that can be stably and permanently modified in their genome open a broad horizon in the world of in vitro modeling. The malaria field is gaining new opportunities from this important breakthrough and novel tools were adapted and opened new frontiers for malaria research. In addition to the new in vitro systems, in recent years there were also significant advances in the development of new animal models that allows studying the entire cell cycle of human malaria. In this paper, we review the different protocols available to study human Plasmodium species either by using stem cell or alternative animal models.

  1. Stochastic biophysical modeling of irradiated cells

    CERN Document Server

    Fornalski, Krzysztof Wojciech

    2014-01-01

    The paper presents a computational stochastic model of virtual cells irradiation, based on Quasi-Markov Chain Monte Carlo method and using biophysical input. The model is based on a stochastic tree of probabilities for each cell of the entire colony. Biophysics of the cells is described by probabilities and probability distributions provided as the input. The adaptation of nucleation and catastrophe theories, well known in physics, yields sigmoidal relationships for carcinogenic risk as a function of the irradiation. Adaptive response and bystander effect, incorporated into the model, improves its application. The results show that behavior of virtual cells can be successfully modeled, e.g. cancer transformation, creation of mutations, radioadaptation or radiotherapy. The used methodology makes the model universal and practical for simulations of general processes. Potential biophysical curves and relationships are also widely discussed in the paper. However, the presented theoretical model does not describe ...

  2. Stellar population synthesis models between 2.5 and 5 {\\mu}m based on the empirical IRTF stellar library

    CERN Document Server

    Röck, B; Peletier, R F; Knapen, J H; Falcón-Barroso, J

    2015-01-01

    We present the first single-burst stellar population models in the infrared wavelength range between 2.5 and 5 {\\mu}m which are exclusively based on empirical stellar spectra. Our models take as input 180 spectra from the stellar IRTF (Infrared Telescope Facility) library. Our final single-burst stellar population models are calculated based on two different sets of isochrones and various types of initial mass functions of different slopes, ages larger than 1 Gyr and metallicities between [Fe/H] = -0.70 and 0.26. They are made available online to the scientific community on the MILES web page. We analyse the behaviour of the Spitzer [3.6]-[4.5] colour calculated from our single stellar population models and find only slight dependences on both metallicity and age. When comparing to the colours of observed early-type galaxies, we find a good agreement for older, more massive galaxies that resemble a single-burst population. Younger, less massive and more metal-poor galaxies show redder colours with respect to ...

  3. Challenges in structural approaches to cell modeling.

    Science.gov (United States)

    Im, Wonpil; Liang, Jie; Olson, Arthur; Zhou, Huan-Xiang; Vajda, Sandor; Vakser, Ilya A

    2016-07-31

    Computational modeling is essential for structural characterization of biomolecular mechanisms across the broad spectrum of scales. Adequate understanding of biomolecular mechanisms inherently involves our ability to model them. Structural modeling of individual biomolecules and their interactions has been rapidly progressing. However, in terms of the broader picture, the focus is shifting toward larger systems, up to the level of a cell. Such modeling involves a more dynamic and realistic representation of the interactomes in vivo, in a crowded cellular environment, as well as membranes and membrane proteins, and other cellular components. Structural modeling of a cell complements computational approaches to cellular mechanisms based on differential equations, graph models, and other techniques to model biological networks, imaging data, etc. Structural modeling along with other computational and experimental approaches will provide a fundamental understanding of life at the molecular level and lead to important applications to biology and medicine. A cross section of diverse approaches presented in this review illustrates the developing shift from the structural modeling of individual molecules to that of cell biology. Studies in several related areas are covered: biological networks; automated construction of three-dimensional cell models using experimental data; modeling of protein complexes; prediction of non-specific and transient protein interactions; thermodynamic and kinetic effects of crowding; cellular membrane modeling; and modeling of chromosomes. The review presents an expert opinion on the current state-of-the-art in these various aspects of structural modeling in cellular biology, and the prospects of future developments in this emerging field.

  4. Synthesis of dihydropyrimidin-2-one/thione library and cytotoxic activity against the human U138-MG and Rat C6 glioma cell lines

    Energy Technology Data Exchange (ETDEWEB)

    Canto, Romulo F.S.; Eifler-Lima, Vera Lucia [Universidade Federal do Rio Grande do Sul (LaSOM/PPGCF/UFRGS), Porto Alegre, RS (Brazil). Programa de Pos Graduacao em Ciencias Farmaceuticas. Lab. de Sintese Organica; Bernardi, Andressa; Battastini, Ana Maria O. [Universidade Federal do Rio Grande do Sul (ICBS/UFRGS), Porto Alegre, RS (Brazil). Inst. de Ciencias Basicas da Saude; Russowsky, Dennis [Universidade Federal do Rio Grande do Sul (IQ/UFRGS), Porto Alegre, RS (Brazil). Inst. de Quimica

    2011-07-01

    Two series of 4-aryl-3,4-dihydropyrimidin-2(1H)-(thio)ones including monastrol (1a), have been synthesized by an environment-friendly methodology based on the combined use of citric acid or oxalic acid and TEOF (triethylorthoformate). The library was evaluated as inhibitor of cell proliferation on two glioma cell lines (human-U138-MG and Rat-C6). The compounds derived from thiourea 1f and 1d were more cytotoxic than monastrol. The compound derived from urea 2d showed the highest cytotoxic activity among the analyzed compounds. (author)

  5. America's Star Libraries

    Science.gov (United States)

    Lyons, Ray; Lance, Keith Curry

    2009-01-01

    "Library Journal"'s new national rating of public libraries, the "LJ" Index of Public Library Service, identifies 256 "star" libraries. It rates 7,115 public libraries. The top libraries in each group get five, four, or three Michelin guide-like stars. All included libraries, stars or not, can use their scores to learn from their peers and improve…

  6. COMBINATORIAL LIBRARIES

    DEFF Research Database (Denmark)

    1997-01-01

    The invention provides a method for the production of a combinatorial library of compound of general formula (I) using solid phase methodologies. The cleavage of the array of immobilised compounds of the phthalimido type from the solid support matrix is accomplished by using an array of dinucleop......The invention provides a method for the production of a combinatorial library of compound of general formula (I) using solid phase methodologies. The cleavage of the array of immobilised compounds of the phthalimido type from the solid support matrix is accomplished by using an array of...... dinucleophiles, e.g. hydrazines (hydrazinolysis) or N-hydroxylamines, whereby a combinatorial dimension is introduced in the cleavage step. The invention also provides a compound library....

  7. One-Step Recovery of scFv Clones from High-Throughput Sequencing-Based Screening of Phage Display Libraries Challenged to Cells Expressing Native Claudin-1

    Science.gov (United States)

    Sasso, Emanuele; Paciello, Rolando; D'Auria, Francesco; Riccio, Gennaro; Froechlich, Guendalina; Cortese, Riccardo; Nicosia, Alfredo; De Lorenzo, Claudia; Zambrano, Nicola

    2015-01-01

    Expanding the availability of monoclonal antibodies interfering with hepatitis C virus infection of hepatocytes is an active field of investigation within medical biotechnologies, to prevent graft reinfection in patients subjected to liver transplantation and to overcome resistances elicited by novel antiviral drugs. In this paper, we describe a complete pipeline for screening of phage display libraries of human scFvs against native Claudin-1, a tight-junction protein involved in hepatitis C virus infection, expressed on the cell surface of human hepatocytes. To this aim, we implemented a high-throughput sequencing approach for library screening, followed by a simple and effective strategy to recover active binder clones from enriched sublibraries. The recovered clones were successfully converted to active immunoglobulins, thus demonstrating the effectiveness of the whole procedure. This novel approach can guarantee rapid and cheap isolation of antibodies for virtually any native antigen involved in human diseases, for therapeutic and/or diagnostic applications. PMID:26649313

  8. One-Step Recovery of scFv Clones from High-Throughput Sequencing-Based Screening of Phage Display Libraries Challenged to Cells Expressing Native Claudin-1

    Directory of Open Access Journals (Sweden)

    Emanuele Sasso

    2015-01-01

    Full Text Available Expanding the availability of monoclonal antibodies interfering with hepatitis C virus infection of hepatocytes is an active field of investigation within medical biotechnologies, to prevent graft reinfection in patients subjected to liver transplantation and to overcome resistances elicited by novel antiviral drugs. In this paper, we describe a complete pipeline for screening of phage display libraries of human scFvs against native Claudin-1, a tight-junction protein involved in hepatitis C virus infection, expressed on the cell surface of human hepatocytes. To this aim, we implemented a high-throughput sequencing approach for library screening, followed by a simple and effective strategy to recover active binder clones from enriched sublibraries. The recovered clones were successfully converted to active immunoglobulins, thus demonstrating the effectiveness of the whole procedure. This novel approach can guarantee rapid and cheap isolation of antibodies for virtually any native antigen involved in human diseases, for therapeutic and/or diagnostic applications.

  9. Modeling cell behavior: moving beyond intuition

    Directory of Open Access Journals (Sweden)

    Mario Jolicoeur

    2014-04-01

    Full Text Available In the context of the launching of this new journal, we propose a forum to the community of researchers interested and involved in, or even simply questioning the why, what, how, and when of modeling cell or cell culture behavior. To start the discussion, we review some of the usual questions we are routinely asked on the pertinence of modeling cell behavior, and on who might benefit from conducting such work. To draw a global portrait, throughout this text we refer the reader to handbooks introducing the basics of modeling a biosystem, as well as to selected works that can help visualize the broad fields of applications.

  10. IDENTIFICATION OF ACTIVE BACTERIAL COMMUNITIES IN A MODEL DRINKING WATER BIOFILM SYSTEM USING 16S RRNA-BASED CLONE LIBRARIES

    Science.gov (United States)

    Recent phylogenetic studies have used DNA as the target molecule for the development of environmental 16S rDNA clone libraries. As DNA may persist in the environment, DNA-based libraries cannot be used to identify metabolically active bacteria in water systems. In this study, a...

  11. The Dreyfus Model of Skill Acquisition: A Career Development Framework for Succession Planning and Management in Academic Libraries

    Science.gov (United States)

    Hall-Ellis, Sylvia D.; Grealy, Deborah S.

    2013-01-01

    Succession planning and management are critical parts of strategic planning for libraries in the twenty-first century. Making certain that the right people are in place with the prerequisite training and experience to assume leadership positions is a vital part of strategic planning and talent management in libraries that rely on teams of people…

  12. Towards an animal model of ovarian cancer: cataloging chicken blood proteins using combinatorial peptide ligand libraries coupled with shotgun proteomic analysis for translational research.

    Science.gov (United States)

    Ma, Yingying; Sun, Zeyu; de Matos, Ricardo; Zhang, Jing; Odunsi, Kunle; Lin, Biaoyang

    2014-05-01

    Epithelial ovarian cancer is the most deadly gynecological cancer around the world, with high morbidity in industrialized countries. Early diagnosis is key in reducing its morbidity rate. Yet, robust biomarkers, diagnostics, and animal models are still limited for ovarian cancer. This calls for broader omics and systems science oriented diagnostics strategies. In this vein, the domestic chicken has been used as an ovarian cancer animal model, owing to its high rate of developing spontaneous epithelial ovarian tumors. Chicken blood has thus been considered a surrogate reservoir from which cancer biomarkers can be identified. However, the presence of highly abundant proteins in chicken blood has compromised the applicability of proteomics tools to study chicken blood owing to a lack of immunodepletion methods. Here, we demonstrate that a combinatorial peptide ligand library (CPLL) can efficiently remove highly abundant proteins from chicken blood samples, consequently doubling the number of identified proteins. Using an integrated CPLL-1DGE-LC-MSMS workflow, we identified a catalog of 264 unique proteins. Functional analyses further suggested that most proteins were coagulation and complement factors, blood transport and binding proteins, immune- and defense-related proteins, proteases, protease inhibitors, cellular enzymes, or cell structure and adhesion proteins. Semiquantitative spectral counting analysis identified 10 potential biomarkers from the present chicken ovarian cancer model. Additionally, many human homologs of chicken blood proteins we have identified have been independently suggested as diagnostic biomarkers for ovarian cancer, further triangulating our novel observations reported here. In conclusion, the CPLL-assisted proteomic workflow using the chicken ovarian cancer model provides a feasible platform for translational research to identify ovarian cancer biomarkers and understand ovarian cancer biology. To the best of our knowledge, we report here

  13. On a poroviscoelastic model for cell crawling

    KAUST Repository

    Kimpton, L. S.

    2014-02-08

    In this paper a minimal, one-dimensional, two-phase, viscoelastic, reactive, flow model for a crawling cell is presented. Two-phase models are used with a variety of constitutive assumptions in the literature to model cell motility. We use an upper-convected Maxwell model and demonstrate that even the simplest of two-phase, viscoelastic models displays features relevant to cell motility. We also show care must be exercised in choosing parameters for such models as a poor choice can lead to an ill-posed problem. A stability analysis reveals that the initially stationary, spatially uniform strip of cytoplasm starts to crawl in response to a perturbation which breaks the symmetry of the network volume fraction or network stress. We also demonstrate numerically that there is a steady travelling-wave solution in which the crawling velocity has a bell-shaped dependence on adhesion strength, in agreement with biological observation.

  14. Planetary Image Geometry Library

    Science.gov (United States)

    Deen, Robert C.; Pariser, Oleg

    2010-01-01

    The Planetary Image Geometry (PIG) library is a multi-mission library used for projecting images (EDRs, or Experiment Data Records) and managing their geometry for in-situ missions. A collection of models describes cameras and their articulation, allowing application programs such as mosaickers, terrain generators, and pointing correction tools to be written in a multi-mission manner, without any knowledge of parameters specific to the supported missions. Camera model objects allow transformation of image coordinates to and from view vectors in XYZ space. Pointing models, specific to each mission, describe how to orient the camera models based on telemetry or other information. Surface models describe the surface in general terms. Coordinate system objects manage the various coordinate systems involved in most missions. File objects manage access to metadata (labels, including telemetry information) in the input EDRs and RDRs (Reduced Data Records). Label models manage metadata information in output files. Site objects keep track of different locations where the spacecraft might be at a given time. Radiometry models allow correction of radiometry for an image. Mission objects contain basic mission parameters. Pointing adjustment ("nav") files allow pointing to be corrected. The object-oriented structure (C++) makes it easy to subclass just the pieces of the library that are truly mission-specific. Typically, this involves just the pointing model and coordinate systems, and parts of the file model. Once the library was developed (initially for Mars Polar Lander, MPL), adding new missions ranged from two days to a few months, resulting in significant cost savings as compared to rewriting all the application programs for each mission. Currently supported missions include Mars Pathfinder (MPF), MPL, Mars Exploration Rover (MER), Phoenix, and Mars Science Lab (MSL). Applications based on this library create the majority of operational image RDRs for those missions. A

  15. The Evolution of the Theory of Library x.0 Reasoning and Model Construction%关于Library x.0理论的演变推理与模型构建

    Institute of Scientific and Technical Information of China (English)

    路茂林

    2011-01-01

    通过对从Library 1.0到Library 2.0、Library x.0的演变过程,提出了图书馆球行关系模型,并在此基础上通过空间拓展模型对Library 1.0到Library x.0的理论作了演变推理,构建出Library 2.0理论的未来发展趋势--Library x.0,并提出了Library x.0的涵义,为Library 2.0理论的延续作了启发性的研究.

  16. Development of probabilistic risk analysis library

    International Nuclear Information System (INIS)

    We developed a library that is designed to perform level 1 Probabilistic Risk Analysis using Binary Decision Diagram (BDD). In particular, our goal is to develop a library that will allow Japanese electric utilities to take the advantages of BDD that can solve Event Tree (ET) and Fault Tree (FT) models analytically. Using BDD, the library supports negation in FT which allows more flexible modeling of ET/FT. The library is written by C++ within an object-oriented framework using open source software. The library itself is a header-only library so that Japanese electric utilities can take advantages of its transparency to speed up development and to build their own software for their specific needs. In this report, the basic capabilities of the library is briefly described. In addition, several applications of the library are demonstrated including validation of MCS evaluation of PRA model and evaluation of corrective and preventive maintenance considering common cause failure. (author)

  17. A Structured Population Model of Cell Differentiation

    CERN Document Server

    Doumic, Marie; Perthame, Benoit; Zubelli, Jorge P

    2010-01-01

    We introduce and analyze several aspects of a new model for cell differentiation. It assumes that differentiation of progenitor cells is a continuous process. From the mathematical point of view, it is based on partial differential equations of transport type. Specifically, it consists of a structured population equation with a nonlinear feedback loop. This models the signaling process due to cytokines, which regulate the differentiation and proliferation process. We compare the continuous model to its discrete counterpart, a multi-compartmental model of a discrete collection of cell subpopulations recently proposed by Marciniak-Czochra et al. in 2009 to investigate the dynamics of the hematopoietic system. We obtain uniform bounds for the solutions, characterize steady state solutions, and analyze their linearized stability. We show how persistence or extinction might occur according to values of parameters that characterize the stem cells self-renewal. We also perform numerical simulations and discuss the q...

  18. Screening of human tumor antigens for CD4 T cell epitopes by combination of HLA-transgenic mice, recombinant adenovirus and antigen peptide libraries.

    Directory of Open Access Journals (Sweden)

    Wolfram Osen

    Full Text Available BACKGROUND: As tumor antigen-specific CD4+ T cells can mediate strong therapeutic anti-tumor responses in melanoma patients we set out to establish a comprehensive screening strategy for the identification of tumor-specific CD4+ T cell epitopes suitable for detection, isolation and expansion of tumor-reactive T cells from patients. METHODS AND FINDINGS: To scan the human melanoma differentiation antigens TRP-1 and TRP-2 for HLA-DRB1*0301-restricted CD4+ T cell epitopes we applied the following methodology: Splenocytes of HLA-DRB1*0301-transgenic mice immunized with recombinant adenovirus encoding TRP-1 (Ad5.TRP-1 or TRP-2 (Ad5.TRP-2 were tested for their T cell reactivity against combinatorial TRP-1- and TRP-2-specific peptide libraries. CD4+ T cell epitopes thus identified were validated in the human system by stimulation of peripheral blood mononuclear cells (PBMC from healthy donors and melanoma patients. Using this strategy we observed that recombinant Ad5 induced strong CD4+ T cell responses against the heterologous tumor antigens. In Ad5.TRP-2-immunized mice CD4+ T cell reactivity was detected against the known HLA-DRB1*0301-restricted TRP-2(60-74 epitope and against the new epitope TRP-2(149-163. Importantly, human T cells specifically recognizing target cells loaded with the TRP-2(149-163-containing library peptide or infected with Ad5.TRP-2 were obtained from healthy individuals, and short term in vitro stimulation of PBMC revealed the presence of epitope-reactive CD4+ T cells in melanoma patients. Similarly, immunization of mice with Ad5.TRP-1 induced CD4+ T cell responses against TRP-1-derived peptides that turned out to be recognized also by human T cells, resulting in the identification of TRP-1(284-298 as a new HLA-DRB1*0301-restricted CD4+ T cell epitope. CONCLUSIONS: Our screening approach identified new HLA-DRB1*0301-restricted CD4+ T cell epitopes derived from melanoma antigens. This strategy is generally applicable to target

  19. The DSET Tool Library: A software approach to enable data exchange between climate system models

    Energy Technology Data Exchange (ETDEWEB)

    McCormick, J. [Lawrence Livermore National Lab., CA (United States)

    1994-12-01

    Climate modeling is a computationally intensive process. Until recently computers were not powerful enough to perform the complex calculations required to simulate the earth`s climate. As a result standalone programs were created that represent components of the earth`s climate (e.g., Atmospheric Circulation Model). However, recent advances in computing, including massively parallel computing, make it possible to couple the components forming a complete earth climate simulation. The ability to couple different climate model components will significantly improve our ability to predict climate accurately and reliably. Historically each major component of the coupled earth simulation is a standalone program designed independently with different coordinate systems and data representations. In order for two component models to be coupled, the data of one model must be mapped to the coordinate system of the second model. The focus of this project is to provide a general tool to facilitate the mapping of data between simulation components, with an emphasis on using object-oriented programming techniques to provide polynomial interpolation, line and area weighting, and aggregation services.

  20. Modeling to Optimize Terminal Stem Cell Differentiation

    Directory of Open Access Journals (Sweden)

    G. Ian Gallicano

    2013-01-01

    Full Text Available Embryonic stem cell (ESC, iPCs, and adult stem cells (ASCs all are among the most promising potential treatments for heart failure, spinal cord injury, neurodegenerative diseases, and diabetes. However, considerable uncertainty in the production of ESC-derived terminally differentiated cell types has limited the efficiency of their development. To address this uncertainty, we and other investigators have begun to employ a comprehensive statistical model of ESC differentiation for determining the role of intracellular pathways (e.g., STAT3 in ESC differentiation and determination of germ layer fate. The approach discussed here applies the Baysian statistical model to cell/developmental biology combining traditional flow cytometry methodology and specific morphological observations with advanced statistical and probabilistic modeling and experimental design. The final result of this study is a unique tool and model that enhances the understanding of how and when specific cell fates are determined during differentiation. This model provides a guideline for increasing the production efficiency of therapeutically viable ESCs/iPSCs/ASC derived neurons or any other cell type and will eventually lead to advances in stem cell therapy.

  1. Evaluación de las bibliotecas digitales: su teoría y modelos Theory and models of assessment of digital libraries

    Directory of Open Access Journals (Sweden)

    Araceli Noguez-Ortiz

    2010-12-01

    Full Text Available Para entender cabalmente la biblioteca digital se requiere no sólo captar su concepto y caracterización, también es necesario relacionarla con los conceptos teóricos de modelo y evaluación; el estudio combinado de estas dos facetas nos lleva a comprender la combinación precisa: teoría de los modelos, modelos de evaluación y por último al modelo de evaluación de las bibliotecas digitales.To fully understand the digital library, one must grasp not only the concept and its features, but also relate it to theoretical modeling and assessment concepts. The study of these two characteristics helps provide an understanding of how modeling theory and assessment models combine to yield the specific evaluation model for digital libraries.

  2. Engineered Models of Confined Cell Migration.

    Science.gov (United States)

    Paul, Colin D; Hung, Wei-Chien; Wirtz, Denis; Konstantopoulos, Konstantinos

    2016-07-11

    Cells in the body are physically confined by neighboring cells, tissues, and the extracellular matrix. Although physical confinement modulates intracellular signaling and the underlying mechanisms of cell migration, it is difficult to study in vivo. Furthermore, traditional two-dimensional cell migration assays do not recapitulate the complex topographies found in the body. Therefore, a number of experimental in vitro models that confine and impose forces on cells in well-defined microenvironments have been engineered. We describe the design and use of microfluidic microchannel devices, grooved substrates, micropatterned lines, vertical confinement devices, patterned hydrogels, and micropipette aspiration assays for studying cell responses to confinement. Use of these devices has enabled the delineation of changes in cytoskeletal reorganization, cell-substrate adhesions, intracellular signaling, nuclear shape, and gene expression that result from physical confinement. These assays and the physiologically relevant signaling pathways that have been elucidated are beginning to have a translational and clinical impact. PMID:27420571

  3. Intelligent Libraries and Apomediators: Distinguishing between Library 3.0 and Library 2.0

    Science.gov (United States)

    Kwanya, Tom; Stilwell, Christine; Underwood, Peter G.

    2013-01-01

    Using the "point oh" naming system for developments in librarianship is attracting debate about its appropriateness, basis and syntax and the meaning and potential of Library 2.0. Now a new term, Library 3.0, has emerged. Is there is any significant difference between the two models? Using documentary analysis to explore the terms, the…

  4. Mathematical model of a cell size checkpoint.

    Directory of Open Access Journals (Sweden)

    Marco Vilela

    Full Text Available How cells regulate their size from one generation to the next has remained an enigma for decades. Recently, a molecular mechanism that links cell size and cell cycle was proposed in fission yeast. This mechanism involves changes in the spatial cellular distribution of two proteins, Pom1 and Cdr2, as the cell grows. Pom1 inhibits Cdr2 while Cdr2 promotes the G2 → M transition. Cdr2 is localized in the middle cell region (midcell whereas the concentration of Pom1 is highest at the cell tips and declines towards the midcell. In short cells, Pom1 efficiently inhibits Cdr2. However, as cells grow, the Pom1 concentration at midcell decreases such that Cdr2 becomes activated at some critical size. In this study, the chemistry of Pom1 and Cdr2 was modeled using a deterministic reaction-diffusion-convection system interacting with a deterministic model describing microtubule dynamics. Simulations mimicked experimental data from wild-type (WT fission yeast growing at normal and reduced rates; they also mimicked the behavior of a Pom1 overexpression mutant and WT yeast exposed to a microtubule depolymerizing drug. A mechanism linking cell size and cell cycle, involving the downstream action of Cdr2 on Wee1 phosphorylation, is proposed.

  5. Medicine's Library Lifeline.

    Science.gov (United States)

    Shipman, Jean; Homan, Michael

    2003-01-01

    Discusses how librarians in the new role of "informationist" can help doctors and researches of medical information. Describes existing models of the informationist; potential benefits of working across professional boundaries outside the library; professional requirements; and unresolved issues for the new role, including potential licensing…

  6. Simple screening method for autoantigen proteins using the N-terminal biotinylated protein library produced by wheat cell-free synthesis.

    Science.gov (United States)

    Matsuoka, Kazuhiro; Komori, Hiroaki; Nose, Masato; Endo, Yaeta; Sawasaki, Tatsuya

    2010-08-01

    Autoimmune diseases are a heterogeneous group of diseases characterized by immune reactions against either a major or a limited number of the bodies own autoantigens, causing inflammation and damage to tissues and organs. Thus, identification of autoantigens is an important first step to understanding autoimmune diseases. Here we demonstrate a simple screening method for identification of autoantigens reacting with patient serum antibodies by combination of an N-terminal biotinylated protein library (BPL), produced using a wheat cell-free protein production system, and a commercially available luminescence system. Optimization studies using well-characterized autoantigens showed specific interactions between N-terminal biotinylated proteins and antibody that were sensitively detected under homogeneous reaction conditions. In this optimized assay, 1 microL of the translation mixture expressing the biotinylated proteins produced significant luminescence signal by addition of diluted serum between 1:500 and 1:10 000 in 25 microL of reaction volume. For the BPL construction, 214 mouse genes, consisting of 103 well-known autoantigens and 111 genes in the mouse autoimmune susceptibility loci, and the sera of MRL/lpr mouse were used as an autoimmune model. By this screening method, 25 well-known autoantigens and 71 proteins in the loci were identified as autoantigen proteins specifically reacting with sera antibodies. Cross-referencing with the Gene Ontology Database, 26 and 38 of autoantigen proteins were predicted to have nuclear localization and identified as membrane and/or extracellular proteins. The immune reaction of six randomly selected proteins was confirmed by immunoprecipitation and/or immunoblot analyses. Interestingly, three autoantigen proteins were recognized by immunoprecipitation but not by immunoblot analysis. These results suggest that the BPL-based method could provide a simple system for screening of autoantigen proteins and would help with

  7. Generation of a phage-display library of single-domain camelid VH H antibodies directed against Chlamydomonas reinhardtii antigens, and characterization of VH Hs binding cell-surface antigens.

    Science.gov (United States)

    Jiang, Wenzhi; Rosenberg, Julian N; Wauchope, Akelia D; Tremblay, Jacqueline M; Shoemaker, Charles B; Weeks, Donald P; Oyler, George A

    2013-11-01

    Single-domain antibodies (sdAbs) are powerful tools for the detection, quantification, purification and subcellular localization of proteins of interest in biological research. We have generated camelid (Lama pacos) heavy chain-only variable VH domain (VH H) libraries against antigens in total cell lysates from Chlamydomonas reinhardtii. The sdAbs in the sera from immunized animals and VH H antibody domains isolated from the library show specificity to C. reinhardtii and lack of reactivity to antigens from four other algae: Chlorella variabilis, Coccomyxa subellipsoidea, Nannochloropsis oceanica and Thalassiosira pseudonana. Antibodies were produced against a diverse representation of antigens as evidenced by sera ELISA and protein-blot analyses. A phage-display library consisting of the VH H region contained at least 10(6) individual transformants, and thus should represent a wide range of C. reinhardtii antigens. The utility of the phage library was demonstrated by using live C. reinhardtii cells to pan for VH H clones with specific recognition of cell-surface epitopes. The lead candidate VH H clones (designated B11 and H10) bound to C. reinhardtii with EC50 values ≤ 0.5 nm. Treatment of cells with VH H B11 fused to the mCherry or green fluorescent proteins allowed brilliant and specific staining of the C. reinhardtii cell wall and analysis of cell-wall genesis during cell division. Such high-complexity VH H antibody libraries for algae will be valuable tools for algal researchers and biotechnologists.

  8. DOLIB: Distributed Object Library

    Energy Technology Data Exchange (ETDEWEB)

    D`Azevedo, E.F.; Romine, C.H.

    1994-10-01

    This report describes the use and implementation of DOLIB (Distributed Object Library), a library of routines that emulates global or virtual shared memory on Intel multiprocessor systems. Access to a distributed global array is through explicit calls to gather and scatter. Advantages of using DOLIB include: dynamic allocation and freeing of huge (gigabyte) distributed arrays, both C and FORTRAN callable interfaces, and the ability to mix shared-memory and message-passing programming models for ease of use and optimal performance. DOLIB is independent of language and compiler extensions and requires no special operating system support. DOLIB also supports automatic caching of read-only data for high performance. The virtual shared memory support provided in DOLIB is well suited for implementing Lagrangian particle tracking techniques. We have also used DOLIB to create DONIO (Distributed Object Network I/O Library), which obtains over a 10-fold improvement in disk I/O performance on the Intel Paragon.

  9. Identification of a panel of tumor-associated antigens from breast carcinoma cell lines, solid tumors and testis cDNA libraries displayed on lambda phage

    Directory of Open Access Journals (Sweden)

    Cianfriglia Maurizio

    2004-11-01

    Full Text Available Abstract Background Tumor-associated antigens recognized by humoral effectors of the immune system are a very attractive target for human cancer diagnostics and therapy. Recent advances in molecular techniques have led to molecular definition of immunogenic tumor proteins based on their reactivity with autologous patient sera (SEREX. Methods Several high complexity phage-displayed cDNA libraries from breast carcinomas, human testis and breast carcinoma cell lines MCF-7, MDA-MB-468 were constructed. The cDNAs were expressed in the libraries as fusion to bacteriophage lambda protein D. Lambda-displayed libraries were efficiently screened with sera from patients with breast cancer. Results A panel of 21 clones representing 18 different antigens, including eight proteins of unknown function, was identified. Three of these antigens (T7-1, T11-3 and T11-9 were found to be overexpressed in tumors as compared to normal breast. A serological analysis of the 21 different antigens revealed a strong cancer-related profile for at least five clones (T6-2, T6-7, T7-1, T9-21 and T9-27. Conclusions Preliminary results indicate that patient serum reactivity against five of the antigens is associated with tumor disease. The novel T7-1 antigen, which is overexpressed in breast tumors and recognized specifically by breast cancer patient sera, is potentially useful in cancer diagnosis.

  10. A methodology for linking 2D overland flow models with the sewer network model SWMM 5.1 based on dynamic link libraries.

    Science.gov (United States)

    Leandro, Jorge; Martins, Ricardo

    2016-01-01

    Pluvial flooding in urban areas is characterized by a gradually varying inundation process caused by surcharge of the sewer manholes. Therefore urban flood models need to simulate the interaction between the sewer network and the overland flow in order to accurately predict the flood inundation extents. In this work we present a methodology for linking 2D overland flow models with the storm sewer model SWMM 5. SWMM 5 is a well-known free open-source code originally developed in 1971. The latest major release saw its structure re-written in C ++ allowing it to be compiled as a command line executable or through a series of calls made to function inside a dynamic link library (DLL). The methodology developed herein is written inside the same DLL in C + +, and is able to simulate the bi-directional interaction between both models during simulation. Validation is done in a real case study with an existing urban flood coupled model. The novelty herein is that the new methodology can be added to SWMM without the need for editing SWMM's original code. Furthermore, it is directly applicable to other coupled overland flow models aiming to use SWMM 5 as the sewer network model. PMID:27332848

  11. Science and Technology Undergraduate Students' Use of the Internet, Cell Phones and Social Networking Sites to Access Library Information

    Science.gov (United States)

    Salisbury, Lutishoor; Laincz, Jozef; Smith, Jeremy J.

    2012-01-01

    Many academic libraries and publishers have developed mobile-optimized versions of their web sites and catalogs. Almost all database vendors and major journal publishers have provided a way to connect to their resources via the Internet and the mobile web. In light of this pervasive use of the Internet, mobile devices and social networking, this…

  12. Quantifying and resolving multiple vector transformants in S. cerevisiae plasmid libraries

    Directory of Open Access Journals (Sweden)

    Gray Elizabeth C

    2009-11-01

    Full Text Available Abstract Background In addition to providing the molecular machinery for transcription and translation, recombinant microbial expression hosts maintain the critical genotype-phenotype link that is essential for high throughput screening and recovery of proteins encoded by plasmid libraries. It is known that Escherichia coli cells can be simultaneously transformed with multiple unique plasmids and thusly complicate recombinant library screening experiments. As a result of their potential to yield misleading results, bacterial multiple vector transformants have been thoroughly characterized in previous model studies. In contrast to bacterial systems, there is little quantitative information available regarding multiple vector transformants in yeast. Saccharomyces cerevisiae is the most widely used eukaryotic platform for cell surface display, combinatorial protein engineering, and other recombinant library screens. In order to characterize the extent and nature of multiple vector transformants in this important host, plasmid-born gene libraries constructed by yeast homologous recombination were analyzed by DNA sequencing. Results It was found that up to 90% of clones in yeast homologous recombination libraries may be multiple vector transformants, that on average these clones bear four or more unique mutant genes, and that these multiple vector cells persist as a significant proportion of library populations for greater than 24 hours during liquid outgrowth. Both vector concentration and vector to insert ratio influenced the library proportion of multiple vector transformants, but their population frequency was independent of transformation efficiency. Interestingly, the average number of plasmids born by multiple vector transformants did not vary with their library population proportion. Conclusion These results highlight the potential for multiple vector transformants to dominate yeast libraries constructed by homologous recombination. The

  13. An electrostatic model for biological cell division

    CERN Document Server

    Faraggi, Eshel

    2010-01-01

    Probably the most fundamental processes for biological systems is their ability to create themselves through the use of cell division and cell differentiation. In this work a simple physical model is proposed for biological cell division. The model consists of a positive ionic gradient across the cell membrane, and concentration of charge at the nodes of the spindle and on the chromosomes. A simple calculation, based on Coulomb's Law, shows that under such circumstances a chromosome will tend to break up to its constituent chromatids and that the chromatids will be separated by a distance that is an order of thirty percent of the distance between the spindle nodes. Further repulsion between the nodes will tend to stretch the cell and eventually break the cell membrane between the separated chromatids, leading to cell division. The importance of this work is in continuing the understanding of the electromagnetic basis of cell division and providing it with an analytical model. A central implication of this and...

  14. CAD MOSFET MODEL FOR EPROM CELLS

    OpenAIRE

    Ballay, N.; Baylac, B.

    1988-01-01

    A CAD model of NOS transistor suitable for circuit simulation of an EPROM cell during writing cycle is proposed. This model is as close as possible of physics to allow the designer to evaluate different schemes and changes in the process variables. It is reliable in the breakdown mode too and takes into account the gate current induced by channel hot electrons.

  15. A Rotamer Library to Enable Modeling and Design of Peptoid Foldamers

    OpenAIRE

    Renfrew, P. Douglas; Craven, Timothy W.; Butterfoss, Glenn L.; Kirshenbaum, Kent; Bonneau, Richard

    2014-01-01

    Peptoids are a family of synthetic oligomers composed of N-substituted glycine units. Along with other “foldamer” systems, peptoid oligomer sequences can be predictably designed to form a variety of stable secondary structures. It is not yet evident if foldamer design can be extended to reliably create tertiary structure features that mimic more complex biomolecular folds and functions. Computational modeling and prediction of peptoid conformations will likely play a critical role in enabling...

  16. 知识联盟图书馆知识共享模型研究%Research on the Knowledge Sharing Model of Knowkedge Consortia Library

    Institute of Scientific and Technical Information of China (English)

    周艳

    2011-01-01

    通过对当今图书馆的知识共享现状进行分析,讨论研究知识共享的各种影响因素及策略分析,在对原有模型分析研究的基础上提出了新的模型架构,并通过对知识共享模型的研究,探讨提升知识联盟图书馆的意义。%Based on analysis the current status of knowledge sharing of the library,This article discusses the various factors affecting knowledge sharing and policy analysis,proposes new model structure based on the study of the original model.It is to explore the significance of the library to enhance knowledge alliance by the research of knowledge sharing model.

  17. Cardiac Electromechanical Models: From Cell to Organ

    Directory of Open Access Journals (Sweden)

    Natalia A Trayanova

    2011-08-01

    Full Text Available The heart is a multiphysics and multiscale system that has driven the development of the most sophisticated mathematical models at the frontiers of computation physiology and medicine. This review focuses on electromechanical (EM models of the heart from the molecular level of myofilaments to anatomical models of the organ. Because of the coupling in terms of function and emergent behaviors at each level of biological hierarchy, separation of behaviors at a given scale is difficult. Here, a separation is drawn at the cell level so that the first half addresses subcellular/single cell models and the second half addresses organ models. At the subcelluar level, myofilament models represent actin-myosin interaction and Ca-based activation. Myofilament models and their refinements represent an overview of the development in the field. The discussion of specific models emphasizes the roles of cooperative mechanisms and sarcomere length dependence of contraction force, considered the cellular basis of the Frank-Starling law. A model of electrophysiology and Ca handling can be coupled to a myofilament model to produce an EM cell model, and representative examples are summarized to provide an overview of the progression of field. The second half of the review covers organ-level models that require solution of the electrical component as a reaction-diffusion system and the mechanical component, in which active tension generated by the myocytes produces deformation of the organ as described by the equations of continuum mechanics. As outlined in the review, different organ-level models have chosen to use different ionic and myofilament models depending on the specific application; this choice has been largely dictated by compromises between model complexity and computational tractability. The review also addresses application areas of EM models such as cardiac resynchronization therapy and the role of mechano-electric coupling in arrhythmias and

  18. Digital Libraries from Concept to Practice

    CERN Document Server

    Banciu, D

    2007-01-01

    The paper represents the result of research in the field of digital libraries functionalities in the context of new Grid infrastructure support. It is defined a new vector of knowledge society, informational vector - content vector. It presents a Grid European project which includes Romanian partners, and it defines on this base a digital library model which can be applied for the libraries in Romania.

  19. Library History Revisited: Research Areas and Methodology.

    Science.gov (United States)

    Byberg, Lis

    This paper discusses research areas and methods related to library history. The first section outlines research areas, including activities such as services to children in public libraries, mobile libraries, cataloging rules and practice, classification rules and practices, development of bibliographies, organization models, literature offered to…

  20. Library news

    CERN Multimedia

    CERN Library

    2010-01-01

    The CERN Library has been providing electronic access to the "Techniques de l'Ingénieur" database for the past 8 months. As a reminder, this is a multidisciplinary database of over 4000 technical and scientific articles in French, covering a broad range of topics such as mechanical engineering, safety, electronics and the environment. In a few simple steps, you can create your own account, select the types of documents you are interested in and configure your settings so as to receive alerts when articles in your field of activity are published. You can now access this resource from outside CERN using the "remote access to electronic resources" service. Further information is available here. Direct access to the database. Remote access to electronic resources. If you have any questions or comments, don't hesitate to contact us at: library.desk@cern.ch.

  1. A demonstration of CMOS VLSI circuit prototyping in support of the site facility using the 1.2 micron standard cell library developed by National Security Agency

    Science.gov (United States)

    Smith, Edwyn D.

    1991-03-01

    Two silicon CMOS application specific integrated circuits (ASICs), a data generation chip, and a data checker chip were designed. The conversion of the data generator circuitry into a pair of CMOS ASIC chips using the 1.2 micron standard cell library is documented. The logic design of the data checker is discussed. The functions of the control circuitry is described. An accurate estimate of timing relationships is essential to make sure that the logic design performs correctly under practical conditions. Timing and delay information are examined.

  2. Preparation of a high-quality cDNA library from a single-cell quantity of mRNA using chum-RNA.

    Science.gov (United States)

    Nojima, Hiroshi; Tougan, Takahiro

    2011-01-01

    Unlike exponential amplification using polymerase chain reaction (PCR), linear RNA amplification using T7 RNA polymerase is advantageous for genome-wide analysis of gene expression and for cDNA library preparation from single-cell quantities of RNA. However, the use of RNA polymerase requires a large amount of RNA, as the optimum concentration of the substrate (mRNA), or the Michaelis constant (K(m)), is one millionfold higher than the single-cell amount of mRNA. To circumvent this K(m) problem, we designed a small mRNA-like dummy molecule, termed chum-RNA, which can be easily removed after the completion of the reaction. Chum-RNA allowed the preparation of a high-quality cDNA library from single-cell quantities of RNA after four rounds of T7-based linear amplification, without using PCR amplification. The use of chum-RNA may also facilitate quantitative reverse-transcription (qRT)-PCR from small quantities of substrate. PMID:21365481

  3. A Study of the Matrix Management Model of Academic Library%高校图书馆矩阵式业务管理模式探析

    Institute of Scientific and Technical Information of China (English)

    曾朝霞

    2011-01-01

    本文对我国高校大多数图书馆现行的管理模式进行了剖析,提出了纵横结合的矩阵式业务管理模式是高校图书馆管理发展的方向,分析了这种新型管理模式的优越之处,并对如何控制其工作质量,提高工作效率和保持工作的连续性提出了自己的见解。%With the development of computer and network, the traditional vertical management model in academic library turns to be less efficient. Some colleges and universities have started to adopt the matrix management model in order to ensure better service of computerization and virtualization in modern libraries. After analyzing the present popular management models, the paper proposed that the matrix model is most likely the tendency of library management. By analyzing the benefits of this new model , the ideas about the guarantee of its quality, efficiency and continuation are put forward.

  4. Radiobiological modelling with MarCell software

    Energy Technology Data Exchange (ETDEWEB)

    Hasan, J.S.; Jones, T.D.

    1996-10-01

    Jones introduced a bone marrow radiation cell kinetics model with great potential for application in the fields of health physics, radiation research, and medicine. However, until recently, only the model developers have been able to apply it because of the complex array of biological and physical assignments needed for evaluation of a particular radiation exposure protocol. The purpose of this article is to illustrate the use of MarCell (MARrow CELL Kinetics) software for MS-DOS, a user-friendly computer implementation of that mathematical model that allows almost anyone with an elementary knowledge of radiation physics and/or medical procedures to apply the model. A hands-on demonstration of the software will be given by guiding the user through evaluation of a medical total body irradiation protocol and a nuclear fallout scenario. A brief overview of the software is given in the Appendix.

  5. Open Access, Library Subscriptions and Article Processing Charges: Hybrid journals models and issues

    OpenAIRE

    Vijayakumar, J. K.; Tamarkin, Molly

    2016-01-01

    Hybrid journals contains articles behind a pay-wall to be subscribed, as well as papers made open access when author pays article processing charge (APC). In such cases, an Institution will end up paying twice and Publishers tend to double-dip. Discussions and pilot models are emerging on pricing options, such as “offset pricing,” [where APCs are adjusted or discounted with subscription costs as vouchers or reductions in next year subscriptions, APCs beyond the subscription costs are modestl...

  6. Customer service in libraries best practices

    CERN Document Server

    Harmon, Charles

    2013-01-01

    In this book, nine librarians from across the country describe their libraries' best practices in this key area. Their contributions range from all-encompassing customer service policies and models any library can both adapt and be proud of to micro-approaches that emphasize offering excellent user-focused technology planning, picture book arrangement with patrons in mind, Web 2.0 tools to connect users with the library, establishing good service delivery chains, and making your library fantastic for homeschoolers.

  7. Radiobiological modeling with MarCell software

    Energy Technology Data Exchange (ETDEWEB)

    Hasan, J.S.; Jones, T.D. [Oak Ridge National Lab., TN (United States). Health Sciences Research Div.

    1999-01-01

    A nonlinear system of differential equations that models the bone marrow cellular kinetics associated with radiation injury, molecular repair, and compensatory cell proliferation has been extensively documented. Recently, that model has been implemented as MarCell, a user-friendly MS-DOS computer program that allows users with little knowledge of the original model to evaluate complex radiation exposure scenarios. The software allows modeling with the following radiations: tritium beta, 100 kVp X, 250 kVp X, 22 MV X, {sup 60}Co, {sup 137}Cs, 2 MeV electrons, triga neutrons, D-T neutrons, and 3 blends of mixed-field fission radiations. The possible cell lineages are stem, stroma, and leukemia/lymphoma, and the available species include mouse, rat, dog, sheep, swine, burro, and man. An attractive mathematical feature is that any protracted protocol can be expressed as an equivalent prompt dose for either the source used or for a reference, such as 250 kVp X rays or {sup 60}Co. Output from MarCell includes: risk of 30-day mortality; risk of cancer and leukemia based either on cytopenia or compensatory cell proliferation; cell survival plots as a function of time or dose; and 4-week recovery kinetics following treatment. In this article, the program`s applicability and ease of use are demonstrated by evaluating a medical total body irradiation protocol and a nuclear fallout scenario.

  8. Research on Open Knowledge Service Model in University Libraries%大学图书馆开放型知识服务模式研究

    Institute of Scientific and Technical Information of China (English)

    马天舒

    2013-01-01

    It is particularly important to develop the contribution ability of information service for the university libraries in constructing scientific and technological innovation system. Based on relevant literatures, this paper defines the connotation and characteristics of knowledge service and open knowledge service in university library, analyzes the feasibility of open knowledge service, elaborates the con-tents of open knowledge service, puts forward the innovation strategy of the open knowledge service, and establishes four types of knowl-edge service patterns of university knowledge service model, enterprise-library knowledge service model, government-library knowledge service model, and science and technology intermediary knowledge service model. The four types of knowledge service models are admin-istrated separately by Subject Librarian ( CSO), Enterprise Librarians ( CEO), Government Librarians ( CGO), and Patent Librarian (CPO) through the website portal of the university libraries, complementing each other in a symbiotic relationship, and form a knowledge service system in university libraries.%  在构建科技创新体系过程中,大学图书馆信息服务的贡献能力尤为重要。通过查阅相关文献,定义大学图书馆知识服务和开放型知识服务的内涵与特征,分析大学图书馆开放型知识服务的可行性,阐述大学图书馆开放型知识服务的内容,并建立大学知识服务模式、馆企知识服务模式、馆政知识服务模式、科技中介知识服务模式四种知识服务模式,提出开放型知识服务模式的创新策略。四种知识服务模式通过大学图书馆门户网站由学科馆员(CSO)、企业馆员(CEO)、政府馆员(CGO)、专利馆员(CPO)分别管理,相辅相成、互利共生构成大学图书馆知识服务系统。

  9. A novel method for the line-of-response and time-of-flight reconstruction in TOF-PET detectors based on a library of synchronized model signals

    CERN Document Server

    Moskal, P; Bednarski, T; Białas, P; Czerwiński, E; Gajos, A; Kamińska, D; Kapłon, Ł; Kochanowski, A; Korcyl, G; Kowal, J; Kowalski, P; Kozik, T; Krzemień, W; Kubicz, E; Niedźwiecki, Sz; Pałka, M; Raczyński, L; Rudy, Z; Rundel, O; Salabura, P; Sharma, N G; Silarski, M; Słomski, A; Smyrski, J; Strzelecki, A; Wieczorek, A; Wiślicki, W; Zieliński, M

    2014-01-01

    A novel method of hit time and hit position reconstruction in scintillator detectors is described. The method is based on comparison of detector signals with results stored in a library of synchronized model signals registered for a set of well-defined positions of scintillation points. The hit position is reconstructed as the one corresponding to the signal from the library which is most similar to the measurement signal. The time of the interaction is determined as a relative time between the measured signal and the most similar one in the library. A degree of similarity of measured and model signals is defined as the distance between points representing the measurement- and model-signal in the multi-dimensional measurement space. Novelty of the method lies also in the proposed way of synchronization of model signals enabling direct determination of the difference between time-of-flights (TOF) of annihilation quanta from the annihilation point to the detectors. The introduced method was validated using expe...

  10. Random sequencing of an induced Taxus cell cDNA library for identification of clones involved in Taxol biosynthesis

    OpenAIRE

    Jennewein, Stefan; Wildung, Mark R.; Chau, MyDoanh; Walker, Kevin; Croteau, Rodney

    2004-01-01

    Biosynthesis of the anticancer drug Taxol involves 19 enzymatic steps from the universal diterpenoid progenitor geranylgeranyl diphosphate derived by the plastidial methylerythritol phosphate pathway for isoprenoid precursor supply. To gain further insight about Taxol biosynthesis relevant to the improved production of this drug and to draw inferences about the organization, regulation, and origins of this complex natural product pathway, random sequencing of a cDNA library derived from Taxus...

  11. VizieR Online Data Catalog: AGN torus models. SED library (Siebenmorgen+, 2015)

    Science.gov (United States)

    Siebenmorgen, R.; Heymann, F.; Efstathiou, A.

    2015-08-01

    There are 3600 ASCII tables files in two columns format. The first is the wavelength in microns, the second column is the flux in Jy. SEDs are computed for AGNs at a distance of 50Mpc and a luminosity of 1011L⊙. The file names include the five basic model parameters: a) th: The viewing angle corresponding to bins at 86, 80, 73, 67, 60, 52, 43, 33, and 19 degree measured from the pole (z-axis). thx= th1 ,.., th9 b) R : The inner radius of the dusty torus. R= 300, 514, 772, 1000, 1545 in units: (10^15 cm) c) Vc: The cloud volume filling factor. Vc= 1.5, 7.7, 38.5, 77.7 (%). d) Ac: The optical depth (in V) of the individual clouds. Ac= 0, 4.5, 13.5, 45. e) Ad: The optical depth (in V) of the disk midplane. Ad= 0, 30, 100, 300, 1000. Example: File notation. RxxxxVcxxxAcxxxx_Adxxxx.thx R1545Vc777Ac0135_Ad1000.th9 (2 data files).

  12. Large animal models for stem cell therapy

    OpenAIRE

    Harding, John; Roberts, R. Michael; Mirochnitchenko, Oleg

    2013-01-01

    The field of regenerative medicine is approaching translation to clinical practice, and significant safety concerns and knowledge gaps have become clear as clinical practitioners are considering the potential risks and benefits of cell-based therapy. It is necessary to understand the full spectrum of stem cell actions and preclinical evidence for safety and therapeutic efficacy. The role of animal models for gaining this information has increased substantially. There is an urgent need for nov...

  13. Chemical Library Screening and Structure-Function Relationship Studies Identify Bisacodyl as a Potent and Selective Cytotoxic Agent Towards Quiescent Human Glioblastoma Tumor Stem-Like Cells.

    Directory of Open Access Journals (Sweden)

    Maria Zeniou

    Full Text Available Cancer stem-like cells reside in hypoxic and slightly acidic tumor niches. Such microenvironments favor more aggressive undifferentiated phenotypes and a slow growing "quiescent state" which preserves them from chemotherapeutic agents that essentially target proliferating cells. Our objective was to identify compounds active on glioblastoma stem-like cells, including under conditions that mimick those found in vivo within this most severe and incurable form of brain malignancy. We screened the Prestwick Library to identify cytotoxic compounds towards glioblastoma stem-like cells, either in a proliferating state or in more slow-growing "quiescent" phenotype resulting from non-renewal of the culture medium in vitro. Compound effects were assessed by ATP-level determination using a cell-based assay. Twenty active molecules belonging to different pharmacological classes have thus been identified. Among those, the stimulant laxative drug bisacodyl was the sole to inhibit in a potent and specific manner the survival of quiescent glioblastoma stem-like cells. Subsequent structure-function relationship studies led to identification of 4,4'-dihydroxydiphenyl-2-pyridyl-methane (DDPM, the deacetylated form of bisacodyl, as the pharmacophore. To our knowledge, bisacodyl is currently the only known compound targeting glioblastoma cancer stem-like cells in their quiescent, more resistant state. Due to its known non-toxicity in humans, bisacodyl appears as a new potential anti-tumor agent that may, in association with classical chemotherapeutic compounds, participate in tumor eradication.

  14. A Study of Organization Structure Model in Library Strategic Planning%图书馆战略规划组织结构模型的构建

    Institute of Scientific and Technical Information of China (English)

    贾东琴; 肖雪; 柯平; 陈雅茜

    2011-01-01

    战略规划组织模型对于图书馆战略规划的制定和实施具有重要的意义。本文通过引入相关管理理论,考察国外图书馆战略规划实践,分析规划文本和利用实证研究结论进行了模型构建。战略规划组织模型主要由专职机构设置、组织结构、人员组成及职能四方面构成。%It is of great significance to develop strategic planning management model for the development and implement of library strategic planning.The model can be constructed by introducing relevant theory,observing the practice of library strategic planning abr

  15. Libraries for users services in academic libraries

    CERN Document Server

    Alvite, Luisa

    2010-01-01

    This book reviews the quality and evolution of academic library services. It revises service trends offered by academic libraries and the challenge of enhancing traditional ones such as: catalogues, repositories and digital collections, learning resources centres, virtual reference services, information literacy and 2.0 tools.studies the role of the university library in the new educational environment of higher educationrethinks libraries in academic contextredefines roles for academic libraries

  16. Optical models for silicon solar cells

    Energy Technology Data Exchange (ETDEWEB)

    Marshall, T.; Sopori, B. [National Renewable Energy Lab., Golden, CO (United States)

    1995-08-01

    Light trapping is an important design feature for high-efficiency silicon solar cells. Because light trapping can considerably enhance optical absorption, a thinner substrate can be used which, in turn, can lower the bulk carrier recombination and concommitantly increase open-circuit voltage, and fill factor of the cell. The basic concepts of light trapping are similar to that of excitation of an optical waveguide, where a prism or a grating structure increases the phase velocity of the incoming optical wave such that waves propagated within the waveguide are totally reflected at the interfaces. Unfortunately, these concepts break down because the entire solar cell is covered with such a structure, making it necessary to develop new analytical approaches to deal with incomplete light trapping in solar cells. This paper describes two models that analyze light trapping in thick and thin solar cells.

  17. Computational Modeling of Cell Survival Using VHDL

    Directory of Open Access Journals (Sweden)

    Shruti Jain1,

    2010-01-01

    Full Text Available The model for cell survival has been implemented using VeryHigh Speed Integrated Circuit Hardware DescriptionLanguage (VHDL (Xilinx Tool taking three input signals:Tumor necrosis factor-α (TNF, Epidermal growth factor(EGF and Insulin. Cell survival has been regulated by theinteraction of five proteins viz P13K, TNFR1, EGFR, IRS andIKK in a network. In the absence of any one, in protein networkleads to cell death. For the EGF input signal the proteins likeMEK, ERK, AkT, Rac & JNK have been important forregulation of cell survival. Similarly for TNF and Insulin inputsignal proteins like NFκB, AkT, XIAP, JNK, MAP3K & MK2and MEK, ERK, AkT, Rac, mTOR & JNK respectively havebeen important for regulation of cell survival.

  18. SU-E-J-93: Development of Pre-Contoured Human Model Library in DICOM-RT Format for the Epidemiological Study of the Radiotherapy Patients

    International Nuclear Information System (INIS)

    Purpose: Prior to 3D conformal radiation therapy planning, patient anatomy information was mostly limited to 2D beams-eye-view from the conventional simulator. To analyze the outcomes of such treatments for radiation late effects, 3D computational human models are often used in commercial treatment planning systems (TPSs). However, several underlying difficulties such as time-consuming manual delineation procedures of a large number of structures in the model have always limited its applications. Primary objective of this work was to develop a human model library for the epidemiological study by converting 3D-surface model organs to DICOM-RT format (DICOM-RT structure) using an in-house built software. We converted the ICRP reference human models to DICOM-RT models, which can be readily adopted for various dose calculations. Methods: MATLAB based code were utilized to convert the contour drawings extracted in text-format from the 3D graphic-tool, Rhinoceros into DICOM-RT structure format for 50 different organs of each model using a 16GB dual-core processor. The conversion periods were measured for each DICOM-RT models, and the reconstructed structure volumes were validated against the original 3D-surface models in the TPS. Ten reference hybrid whole-body models (8-pediatric and 2-adults) were automatically processed to create DICOM-RT computational human model library. Results: Mean contour conversion period was found to be 580 (N=2) and 394.5 (N=8) seconds for 50 organs in the adult and pediatric models respectively. A good agreement for large organs (NRMSD <1.0%) and small organs (NRMSD <7.7%) was also observed between the original volumes and corresponding DICOM-RT structure volumes of the organs. Conclusion: The ICRP reference human models were converted into DICOM-RT format to support the epidemiological study using a large cohort of conventional radiotherapy patients. Due to its DICOM-compatibility, the library may be implemented to many other different

  19. SU-E-J-93: Development of Pre-Contoured Human Model Library in DICOM-RT Format for the Epidemiological Study of the Radiotherapy Patients

    Energy Technology Data Exchange (ETDEWEB)

    Pyakuryal, A; Lee, C [National Cancer Institute, Rockville, MD (United States); Lee, C [University of Michigan, Ann Arbor, MI (United States); Pelletier, C [East Carolina University, Greenville, NC (United States); Jung, J [East Carolina Univ, Greenville, NC (United States)

    2015-06-15

    Purpose: Prior to 3D conformal radiation therapy planning, patient anatomy information was mostly limited to 2D beams-eye-view from the conventional simulator. To analyze the outcomes of such treatments for radiation late effects, 3D computational human models are often used in commercial treatment planning systems (TPSs). However, several underlying difficulties such as time-consuming manual delineation procedures of a large number of structures in the model have always limited its applications. Primary objective of this work was to develop a human model library for the epidemiological study by converting 3D-surface model organs to DICOM-RT format (DICOM-RT structure) using an in-house built software. We converted the ICRP reference human models to DICOM-RT models, which can be readily adopted for various dose calculations. Methods: MATLAB based code were utilized to convert the contour drawings extracted in text-format from the 3D graphic-tool, Rhinoceros into DICOM-RT structure format for 50 different organs of each model using a 16GB dual-core processor. The conversion periods were measured for each DICOM-RT models, and the reconstructed structure volumes were validated against the original 3D-surface models in the TPS. Ten reference hybrid whole-body models (8-pediatric and 2-adults) were automatically processed to create DICOM-RT computational human model library. Results: Mean contour conversion period was found to be 580 (N=2) and 394.5 (N=8) seconds for 50 organs in the adult and pediatric models respectively. A good agreement for large organs (NRMSD <1.0%) and small organs (NRMSD <7.7%) was also observed between the original volumes and corresponding DICOM-RT structure volumes of the organs. Conclusion: The ICRP reference human models were converted into DICOM-RT format to support the epidemiological study using a large cohort of conventional radiotherapy patients. Due to its DICOM-compatibility, the library may be implemented to many other different

  20. The Future of Web 2.0 in Libraries and Information Centers: Open Discussion

    OpenAIRE

    Tonta, Yaşar; Madran , Orçun

    2009-01-01

    In this presentation, the future of Web 2.0 in libraries and information centers is disccussed. Library 2.0 concept model, library discussion, library management environment, applicable Web 2.0 technologies, services for the next-generation library, social media use, future of libraries with an optimist and pessimist view, plus issues are the headings in the presentation.

  1. Towards a suite of test cases and a pycomodo library to assess and improve numerical methods in ocean models

    Science.gov (United States)

    Garnier, Valérie; Honnorat, Marc; Benshila, Rachid; Boutet, Martial; Cambon, Gildas; Chanut, Jérome; Couvelard, Xavier; Debreu, Laurent; Ducousso, Nicolas; Duhaut, Thomas; Dumas, Franck; Flavoni, Simona; Gouillon, Flavien; Lathuilière, Cyril; Le Boyer, Arnaud; Le Sommer, Julien; Lyard, Florent; Marsaleix, Patrick; Marchesiello, Patrick; Soufflet, Yves

    2016-04-01

    bed to continue research in numerical approaches as well as an efficient tool to maintain any oceanic code and assure the users a stamped model in a certain range of hydrodynamical regimes. Thanks to a common netCDF format, this suite is completed with a python library that encompasses all the tools and metrics used to assess the efficiency of the numerical methods. References - Couvelard X., F. Dumas, V. Garnier, A.L. Ponte, C. Talandier, A.M. Treguier (2015). Mixed layer formation and restratification in presence of mesoscale and submesoscale turbulence. Ocean Modelling, Vol 96-2, p 243-253. doi:10.1016/j.ocemod.2015.10.004. - Soufflet Y., P. Marchesiello, F. Lemarié, J. Jouanno, X. Capet, L. Debreu , R. Benshila (2016). On effective resolution in ocean models. Ocean Modelling, in press. doi:10.1016/j.ocemod.2015.12.004

  2. Grey Incidence Analysis (gia): a New Local Method for Modelling Chinese Soil Vis-Nir Spectral Library to Predict Soil Total Nitrogen Content

    Science.gov (United States)

    Wang, Qianlong

    2014-06-01

    This paper introduces a new approach called grey incidence analysis(GIA), by which high accuracy prediction model can be established combined with partial least squares regression(PLSR) to deal with Chinese soil vis-NIR spectral library and estimate soil total nitrogen content in local area. Using spectral matching algorithm such as Mahalanobis distance, spectral angle model (SAM) and spectral correlation fitting(SCF), fuzzy k-means clustering method only based on the spectral data without considering soil total nitrogen content in the library. Soil total nitrogen content have grey uncertainty relationship with each vis-NIR spectral band(400-2500nm). This method combine the soil total nitrogen value with spectrum data when performing spectral matching. In this study, 1661 soil samples in the library were collected from 13 provinces in China, which include Tibet, Xinjiang, Heilongjiang, and Hainan. The samples represent 17 soil groups of the Chinese Soil (Genetic) Classification System. After air-drying and sieving, the diffuse reflectance spectra of the samples were measured under laboratory conditions in the range between 400 and 2500 nm using a portable vis-NIR spectrometer. Hyperspectral inversion model was built based on 104 paddy soil samples in Zhejiang province to predict the soil total nitrogen content. The results show that the GIA-PLSR method presents great potential for predicting soil total nitrogen content in large soil vis-NIR library. The prediction accuracy: R2 = 0.897, RMSEP = 0.028, RPDP = 3.151. This study also show that soil vis-NIR spectroscopy combined with TN value can be used to further improve the prediction performance of spectral models.

  3. Enterprise Reference Library

    Science.gov (United States)

    Bickham, Grandin; Saile, Lynn; Havelka, Jacque; Fitts, Mary

    2011-01-01

    Introduction: Johnson Space Center (JSC) offers two extensive libraries that contain journals, research literature and electronic resources. Searching capabilities are available to those individuals residing onsite or through a librarian s search. Many individuals have rich collections of references, but no mechanisms to share reference libraries across researchers, projects, or directorates exist. Likewise, information regarding which references are provided to which individuals is not available, resulting in duplicate requests, redundant labor costs and associated copying fees. In addition, this tends to limit collaboration between colleagues and promotes the establishment of individual, unshared silos of information The Integrated Medical Model (IMM) team has utilized a centralized reference management tool during the development, test, and operational phases of this project. The Enterprise Reference Library project expands the capabilities developed for IMM to address the above issues and enhance collaboration across JSC. Method: After significant market analysis for a multi-user reference management tool, no available commercial tool was found to meet this need, so a software program was built around a commercial tool, Reference Manager 12 by The Thomson Corporation. A use case approach guided the requirements development phase. The premise of the design is that individuals use their own reference management software and export to SharePoint when their library is incorporated into the Enterprise Reference Library. This results in a searchable user-specific library application. An accompanying share folder will warehouse the electronic full-text articles, which allows the global user community to access full -text articles. Discussion: An enterprise reference library solution can provide a multidisciplinary collection of full text articles. This approach improves efficiency in obtaining and storing reference material while greatly reducing labor, purchasing and

  4. Construction of cDNA representational difference analysis based on two cDNA libraries and identification of garlic inducible expression genes in human gastric cancer cells

    Institute of Scientific and Technical Information of China (English)

    Yong Li; Lin Yang; Jian-Tao Cui; Wen-Mei Li; Rui-Fang Guo; You-Yong Lu

    2002-01-01

    AIM: To elucidate molecular mechanism of chemopreventiveefficacies of garlic against human gastric cancer (HGC):METHODS: HGC cell line BGC823 was treated with Allitridi (akind of garlic extract) and Allitridi-treated and parentalBGC823 cDNA librarles were constructed respectively byusing λZAP Ⅱ vector. cDNA Representatinal DifferenceAnalysis (cDNA RDA) was perfonmed using BamH Ⅰ cutting-site and abundant ~DNA messages provided by the Iibrarles.Northern blot analysls was applied to identifythe obtaineddifference prnducts.RESULTS: Two specific cDNA fragments were obtained andcharacterized to be derived from homo sapiens folatereceptorα (FRα) gene and calcyclin gene respectively.Northern blot results showed a 4-fold increase in FRα geneexpression level and 9-fold increase in calcyclin mRNA levelin BGC823 cells after Allilridi treatment for 72 h.CONCLUSION: The method of cDNA RDA based on cDNAlibraries combines the high specificity of cDNA RDA withabundant cDNA messages in cDNA library; this expands theapplication of cDNA library and increases the specificity ofcDNA RDA. Up-regulstion of FRα gene and calcyclin geneexpressions induced by Allitridi provide valuable molecularevidence for theefficacy of garlic in treating HGC as well asother diseases.

  5. Modeling cell-in-cell structure into its biological significance

    OpenAIRE

    He, M-f; S. Wang; Wang, Y.; Wang, X-N.

    2013-01-01

    Although cell-in-cell structure was noted 100 years ago, the molecular mechanisms of ‘entering' and the destination of cell-in-cell remain largely unclear. It takes place among the same type of cells (homotypic cell-in-cell) or different types of cells (heterotypic cell-in-cell). Cell-in-cell formation affects both effector cells and their host cells in multiple aspects, while cell-in-cell death is under more intensive investigation. Given that cell-in-cell has an important role in maintainin...

  6. Voronoi cell patterns: Theoretical model and applications

    Science.gov (United States)

    González, Diego Luis; Einstein, T. L.

    2011-11-01

    We use a simple fragmentation model to describe the statistical behavior of the Voronoi cell patterns generated by a homogeneous and isotropic set of points in 1D and in 2D. In particular, we are interested in the distribution of sizes of these Voronoi cells. Our model is completely defined by two probability distributions in 1D and again in 2D, the probability to add a new point inside an existing cell and the probability that this new point is at a particular position relative to the preexisting point inside this cell. In 1D the first distribution depends on a single parameter while the second distribution is defined through a fragmentation kernel; in 2D both distributions depend on a single parameter. The fragmentation kernel and the control parameters are closely related to the physical properties of the specific system under study. We use our model to describe the Voronoi cell patterns of several systems. Specifically, we study the island nucleation with irreversible attachment, the 1D car-parking problem, the formation of second-level administrative divisions, and the pattern formed by the Paris Métro stations.

  7. Modeling sarcomagenesis using multipotent mesenchymal stem cells

    Institute of Scientific and Technical Information of China (English)

    Rene Rodriguez; Ruth Rubio; Pablo Menendez

    2012-01-01

    Because of their unique properties,multipotent mesenchymal stem cells (MSCs) represent one of the most promising adult stem cells being used worldwide in a wide array of clinical applications.Overall,compelling evidence supports the long-term safety of ex vivo expanded human MSCs,which do not seem to transform spontaneously.However,experimental data reveal a link between MSCs and cancer,and MSCs have been reported to inhibit or promote tumor growth depending on yet undefined conditions.Interestingly,solid evidence based on transgenic mice and genetic intervention of MSCs has placed these cells as the most likely cell of origin for certain sarcomas.This research area is being increasingly explored to develop accurate MSC-based models of sarcomagenesis,which will be undoubtedly valuable in providing a better understanding about the etiology and pathogenesis of mesenchymal cancer,eventually leading to the development of more specific therapies directed against the sarcoma-initiating cell.Unfortunately,still little is known about the mechanisms underlying MSC transformation and further studies are required to develop bona fide sarcoma models based on human MSCs.Here,we comprehensively review the existing MSC-based models of sarcoma and discuss the most common mechanisms leading to tumoral transformation of MSCs and sarcomagenesis.

  8. Implementing PDA technology in a medical library: experiences in a hospital library and an academic medical center library.

    Science.gov (United States)

    Morgen, Evelyn Breck

    2003-01-01

    Personal digital assistants (PDAs) have grown from being a novelty in the late 1990s to an essential tool for healthcare professionals in the 2000s. This paper describes the experiences of a librarian who implemented PDA technology first in a hospital library, and then at an academic medical center library. It focuses on the role of the library in supporting PDA technology and resources. Included are programmatic issues such as training for library staff and clinicians, and technical issues such as Palm and Windows operating systems. This model could be used in either a hospital or academic health sciences library.

  9. Library Services. Miscellaneous Papers.

    Science.gov (United States)

    International Federation of Library Associations, The Hague (Netherlands).

    Papers on library journal cooperation, interlibrary lending, library services to minorities, and school library media centers, which were presented at the 1983 International Federation of Library Associations (IFLA) conference, include: (1) "The Co-operation between Editors of Library Journals in Socialist Countries," in which Wolfgang Korluss…

  10. Library Research and Statistics.

    Science.gov (United States)

    Lynch, Mary Jo; Brier, David J.; Lebbin, Vickery K.; Halstead, Kent; Fox, Bette-Lee; Kremen, Maya L.; Miller, Marilyn L.; Shontz, Marilyn L.

    1998-01-01

    Provides nine articles: research on libraries and librarianship, 1997; changing faces of library education (ALA-accredited graduate program title changes); number of libraries in the U.S., Canada, and Mexico; highlights of NCES surveys; library acquisition expenditures; price indexes for public and academic libraries; state rankings of selected…

  11. Marketing the Virtual Library

    Science.gov (United States)

    Fagan, Jody Condit

    2009-01-01

    Far more people are familiar with their local public or college library facility than their library's website and online resources. In fact, according to a recent survey, 96% of Americans said they had visited a library in person, but less than one-third have visited their online library. Since everyone agrees that online library resources are…

  12. Biophysical models of transcription in cells

    Science.gov (United States)

    Choubey, Sandeep

    Cells constantly face environmental challenges and deal with them by changing their gene expression patterns. They make decisions regarding which genes to express and which genes not to express based on intra-cellular and environmental cues. These decisions are often made by regulating the process of transcription. While the identities of the different molecules that take part in regulating transcription have been determined for a number of different genes, their dynamics inside the cell are still poorly understood. One key feature of these regulatory dynamics is that the numbers of the bio-molecules involved is typically small, resulting in large temporal fluctuations in transcriptional outputs (mRNA and protein). In this thesis I show that measurements of the cell-to-cell variability of the distribution of transcribing RNA polymerases along a gene provide a previously unexplored method for deciphering the mechanism of its transcription in vivo. First, I propose a simple kinetic model of transcription initiation and elongation from which I calculate transcribing RNA polymerase copy-number fluctuations. I test my theory against published data obtained for yeast genes and propose a novel mechanism of transcription. Rather than transcription being initiated through a single rate-limiting step, as was previously proposed, my single-cell analysis reveals the presence of at least two rate limiting steps. Second, I compute the distribution of inter-polymerase distance distribution along a gene and propose a method for analyzing inter-polymerase distance distributions acquired in experiments. By applying this method to images of polymerases transcribing ribosomal genes in E.coli I show that one model of regulation of these genes is consistent with inter-polymerase distance data while a number of other models are not. The analytical framework described in this thesis can be used to extract quantitative information about the dynamics of transcription from single-cell

  13. The analysis of VH and VL genes repertoires of Fab library built from peripheral B cells of human rabies virus vaccinated donors.

    Science.gov (United States)

    Houimel, Mehdi

    2014-08-01

    A human combinatorial Fab antibody library was generated from immune repertoire based on peripheral B cells of ten rabies virus vaccinated donors. The analysis of random Fab fragments from the unselected library presented some bias of V gene usage towards IGHV-genes and IGLV-gen families. The screening of the Fab library on rabies virus allowed specific human Fab antibody fragments characterized for their gene encoding sequences, binding and specificities to RV. Genetic analysis of selected Fabs indicated that the IGHV and IGLV differ from the germ-line sequence. At the level of nucleotide sequences, the IGHV and IGLV domains were found to share 74-92% and 90-96% homology with sequences encoded by the corresponding human germ-line genes respectively. IGHV domains are characterized most frequently by IGHV3 genes, and large proportions of the anti-RV heavy chain IGHV domains are obtained following a VDJ recombination process that uses IGHD3, IGHD2, IGHD1 and IGHD6 genes. IGHJ3 and IGHJ4 genes are predominantly used in RV-Fab. The IGLV domains are dominated by IGKV1, IGLV1 and IGLV3 genes. Numerous somatic hypermutations in the RV-specific IGHV are detected, but only limited amino acid replacement in most of the RV-specific IGLV particularly in those encoded by J proximal IGLV or IGKV genes are found. Furthermore, IGHV3-IGKV1, IGHV3-IGVL1, and IGHV3-IGLV3 germ-line family pairings are preferentially enriched after the screening on rabies virus.

  14. Research on the Reader Service Models of Public Library under the Network Environment%网络环境下公共图书馆读者服务模式探究

    Institute of Scientific and Technical Information of China (English)

    张涛

    2012-01-01

    This paper analyzes the changes of the reader service work of library under the network environment,discusses the reader service models of public library with the practice,and puts forward some supporting measures of the innovation of reader service model of public library.%分析了网络环境下图书馆读者服务工作的新变化,结合实践探讨了网络环境下公共图书馆的读者服务模式,提出了公共图书馆读者服务模式创新的保障措施。

  15. Research on the Embeded Librarian Service Models of University Libraries%高校图书馆嵌入式馆员服务模式研究

    Institute of Scientific and Technical Information of China (English)

    胡银霞

    2012-01-01

    This paper describes the embedded librarian conception from the start, and analyzes the reason of the rise of embedded librarians. Based on the characteristics of the university library service object ,dissertates the bulding of embedded librarians services models of university libraries, respectively, from embedded faculty, teaching and community in three areas, and pointed out their challenges and coping strategies.%论文从介绍嵌入式馆员的概念着手,分析了嵌入式馆员兴起的缘由。针对高校图书馆服务对象的特点,分别从嵌入院系、教学和社区三个方面,论述了高校嵌入式馆员服务模式的构建,并指出其面临的挑战和应对策略。

  16. Selection Analysis of SNS Model of University Libraries%高校图书馆 SNS 信息服务模式的选取分析

    Institute of Scientific and Technical Information of China (English)

    贾建瑞; 李洁

    2015-01-01

    On the basis of ″ Uses and Gratifications Theory″ ,adopting questionnaire to survey,selecting several more representative university libraries in the applications of different SNS model as the sample,this paper comparatively analyzes the using situation and users′needs,trying to provide reference and advice for the application of SNS in universi-ty libraries.%依据“使用与满足理论”,采用问卷调研,抽取在应用不同 SNS 模式中较具代表性的几所高校图书馆作为样本,对比分析使用情况及用户需求,对高校图书馆选取 SNS 信息服务模式提供一些参考及建议。

  17. Why Japanese publishers are behind in making journals online? : Library needs assesment may bring up new business models : Interview with Wataru Hoshino, director at The Japan Society of Publishing Studies and director editor in chief at The Bunka Tushin

    Science.gov (United States)

    Morita, Utako

    Why Japanese publishers are behind in making journals online? : Library needs assesment may bring up new business models : Interview with Wataru Hoshino, director at The Japan Society of Publishing Studies and director editor in chief at The Bunka Tushin

  18. Library Literacy Programs for English Language Learners. ERIC Digest.

    Science.gov (United States)

    McMurrer, Eileen; Terrill, Lynda

    This digest summarizes the history of public libraries and library literacy programs; describes current delivery models; and discusses initiatives in library literacy, profiling one successful public library program that serves adult English language learners and their families. (Adjunct ERIC Clearinghouse for ESL Literacy Education) (Author/VWL)

  19. National Strategies for Public Library Development

    DEFF Research Database (Denmark)

    Kann-Christensen, Nanna

    2010-01-01

    policies on the local level are discussed. according to the national goals and cultural policies for libraries. This is done in order to point towards a possible relationship between the political means and ends regarding library development. The two models to be discussed are the systems practiced...... by The Danish Agency for Libraries and Media, and The Swedish Arts Council, who both administer development grants to public libraries. The paper identifies both differences and similarities in both the means to (the political agendas) and the objectives of governmental influence in the libraries....

  20. What are Libraries For?

    OpenAIRE

    Hugh McGuire

    2011-01-01

    In the Library with the Lead Pipe welcomes guest author Hugh McGuire, the founder of LibriVox.org, the world’s most prolific publisher of audiobooks (all read by volunteers); Iambik Audio, a commercial audiobook publisher built on a model similar to LibriVox; and PressBooks, a simple digital book-production tool. Hugh served on the Board (2002-2010) of the [...

  1. Novel HIV-1 knockdown targets identified by an enriched kinases/phosphatases shRNA library using a long-term iterative screen in Jurkat T-cells.

    Directory of Open Access Journals (Sweden)

    Sylvie Rato

    Full Text Available HIV-1 is a complex retrovirus that uses host machinery to promote its replication. Understanding cellular proteins involved in the multistep process of HIV-1 infection may result in the discovery of more adapted and effective therapeutic targets. Kinases and phosphatases are a druggable class of proteins critically involved in regulation of signal pathways of eukaryotic cells. Here, we focused on the discovery of kinases and phosphatases that are essential for HIV-1 replication but dispensable for cell viability. We performed an iterative screen in Jurkat T-cells with a short-hairpin-RNA (shRNA library highly enriched for human kinases and phosphatases. We identified 14 new proteins essential for HIV-1 replication that do not affect cell viability. These proteins are described to be involved in MAPK, JNK and ERK pathways, vesicular traffic and DNA repair. Moreover, we show that the proteins under study are important in an early step of HIV-1 infection before viral integration, whereas some of them affect viral transcription/translation. This study brings new insights for the complex interplay of HIV-1/host cell and opens new possibilities for antiviral strategies.

  2. Modeling of Silicon Heterojunction Solar Cells

    Energy Technology Data Exchange (ETDEWEB)

    Luppina, P.; Lugli, P.; Goodnick, S.

    2015-06-14

    Here we present modeling results on crystalline Si/amorphous Si (a-Si) heterojunction solar cells using Sentaurus including various models for defect states in the a-Si barriers, as well as explicit models for the ITO emitter contact. We investigate the impact of the band offsets and barrier heights of the a-Si/c-Si interface, particularly in terms of the open circuit voltage. It is also shown that the solar cell performance is sensitively dependent on the quality of the a-Si in terms of defect states and their distribution, particularly on the emitter side. Finally, we have investigate the role of tunneling and thermionic emission across the heterointerface in terms of transport from the Si to the ITO contact layer

  3. Living Library:The New Reader Service Model of Children’s Library%Living Library:少儿图书馆读者服务新模式

    Institute of Scientific and Technical Information of China (English)

    王祎

    2012-01-01

    Living Library,a new concept of library service,has begun to emerge at home and abroad library circles.This paper analyses the feasibility of children’s library to offer the service of Living Library and elaborates the measures of Living Library service provided by children’s library,in order to make more children’s readers come into library,feel the joy of reading and to broaden the development space of children’s library.%作为一种新型图书馆服务理念,Living Library服务逐渐在国内外图书馆界兴起。分析少儿图书馆开展Living Library服务的可行性,详细阐述少儿图书馆开展Living Library服务的措施,以吸引更多的少儿读者走进图书馆,感受阅读的快乐,拓宽少儿图书馆的发展空间。

  4. Enlightenment of the Organization Model and Core Concept of Living Library for the Library Innovation%Living Library组织模式与核心理念对图书馆创新的启示

    Institute of Scientific and Technical Information of China (English)

    柯丹倩; 吴跃伟; 董克

    2012-01-01

    Living Library是在图书馆的组织下,由特定用户担任Living Book并与其他用户交流从而分享知识和智慧的服务理念与服务方式。Living Library的核心理念是让用户服务用户。Living Library活动的实现需要经历宣传Living Library、招募和组织Living Book、提供Living Book借阅、评估Living Library 4个步骤。LivingLibrary经过10多年的发展,取得了良好的成效,为图书馆服务理念创新带来了启示。%Living library is a kind of service concept and mode organized by libraries,in which special users could exchang with other users as living books,in order to share knowledge and wisdom.The core concept of living library is allowing users to serve for users.There are 4 steps to implement living library services: publicizing living library,recruiting and organizing living books,providing living books,evaluating living library.Living library has achieved good effects through more than ten-year development,and brings inspirations for service concept innovation in libraries.

  5. Non linear behaviour of cell tensegrity models

    Science.gov (United States)

    Alippi, A.; Bettucci, A.; Biagioni, A.; Conclusio, D.; D'Orazio, A.; Germano, M.; Passeri, D.

    2012-05-01

    Tensegrity models for the cytoskeleton structure of living cells is largely used nowadays for interpreting the biochemical response of living tissues to mechanical stresses. Microtubules, microfilaments and filaments are the microscopic cell counterparts of struts (microtubules) and cables (microfilaments and filaments) in the macroscopic world: the formers oppose to compression, the latters to tension, thus yielding an overall structure, light and highly deformable. Specific cell surface receptors, such as integrins, act as the coupling elements that transmit the outside mechanical stress state into the cell body. Reversible finite deformations of tensegrity structures have been widely demonstrated experimentally and in a number of living cell simulations. In the present paper, the bistability behaviour of two general models, the linear bar oscillator and the icosahedron, is studied, as they are both obtained from mathematical simulation, the former, and from larger scale experiments, the latter. The discontinuity in the frequency response of the oscillation amplitude and the lateral bending of the resonance curves are put in evidence, as it grows larger as the driving amplitude increases, respectively.

  6. Agricultural Libraries and Information.

    Science.gov (United States)

    Russell, Keith W., Ed.; Pisa, Maria G., Ed.

    1990-01-01

    Eleven articles address issues relating to agricultural libraries and information, including background on agricultural libraries and information, trend management, document delivery, reference services, user needs and library services, collection development, technologies for international information management, information sources,…

  7. Italian library associations

    Directory of Open Access Journals (Sweden)

    Ksenija Petaros-Kmetec

    2004-01-01

    Full Text Available In Italy, five library associations of national significance function at present. There are special associations of ecclesiastic libraries, prison libraries, architecture libraries and libraries with artistic material. The role of the general national association, covering all types of libraries including documentation centres, is played by the Italian Library Association. It strive for the development of a contemporary Italian library system comparable to international standards, monitors library legislation, promotes education for librarians and keeps the librarians and the broader public informed about the importance of libraries and librarianship for society. The activity and efforts of the association are reflected through their website offering much information and links to similar sites. ILA presents and realises its activities for both, the librarians and the public users. A great deal of actions promoting libraries and the Library Association might be interesting for Slovenia and perhaps transferred to our environment.

  8. Repair-misrepair model of cell survival

    International Nuclear Information System (INIS)

    During the last three years a new model, the repair-misrepair model (RMR) has been proposed, to interpret radiobiological experiments with heavy ions. In using the RMR model it became apparent that some of its features are suitable for handling the effects produced by a variety of environmental agents in addition to ionizing radiation. Two separate sequences of events are assumed to take place in an irradiated cell. The first sequence begins with an initial energy transfer consisting of ionizations and excitations, culminating via fast secondary physical and chemical processes in established macromolecular lesions in essential cell structures. The second sequence contains the responses of the cell to the lesions and consists of the processes of recognition and molecular repair. In normal cells there exists one repair process or at most a few enzymatic repair processes for each essential macromolecular lesion. The enzymatic repair processes may last for hours and minutes, and can be separated in time from the initial physicochemical and later genetic phases

  9. A dynamic model of tomato fruit growth integrating cell division, cell growth and endoreduplication

    NARCIS (Netherlands)

    Fanwoua, J.; Visser, de P.H.B.; Heuvelink, E.; Yin, X.; Struik, P.C.; Marcelis, L.F.M.

    2013-01-01

    In this study, we developed a model of tomato (Solanum lycopersicum L.) fruit growth integrating cell division, cell growth and endoreduplication. The fruit was considered as a population of cells grouped in cell classes differing in their initial cell age and cell mass. The model describes fruit gr

  10. Modeling light trapping in nanostructured solar cells.

    Science.gov (United States)

    Ferry, Vivian E; Polman, Albert; Atwater, Harry A

    2011-12-27

    The integration of nanophotonic and plasmonic structures with solar cells offers the ability to control and confine light in nanoscale dimensions. These nanostructures can be used to couple incident sunlight into both localized and guided modes, enhancing absorption while reducing the quantity of material. Here we use electromagnetic modeling to study the resonances in a solar cell containing both plasmonic metal back contacts and nanostructured semiconductor top contacts, identify the local and guided modes contributing to enhanced absorption, and optimize the design. We then study the role of the different interfaces and show that Al is a viable plasmonic back contact material.

  11. The New Model of Information Literacy Education in University Library%高校图书馆信息素质教育新模式研究

    Institute of Scientific and Technical Information of China (English)

    王运显

    2012-01-01

    University library is one of the main places for information literary education.With brief introduction of the content for university students' information literacy,the article analyzes the theoretical basis,the policy basis and the practices of information literacy education in university library.It also analyzes the status and problems of information literacy education model.And finally it discusses the new model of information literary education in university library,that is,"information hierarchy of needs" model,the "information man" model and the "information university" model.%高校图书馆是对大学生进行信息素质教育的基地之一。论文首先概述了大学生信息素质的内涵;其次从理论依据、政策依据和实践体现三个方面对高校图书馆开展大学生信息素质教育进行了阐释,并对高校图书馆信息素质教育模式的现状和存在的问题进行了分析;最后,对高校图书馆信息教育的新模式,即"信息需求层次"模式、"信息人"模式和"信息大学"模式进行了阐述。

  12. CellLab-CTS 2015: continuous-time stochastic cellular automaton modeling using Landlab

    Science.gov (United States)

    Tucker, Gregory E.; Hobley, Daniel E. J.; Hutton, Eric; Gasparini, Nicole M.; Istanbulluoglu, Erkan; Adams, Jordan M.; Siddartha Nudurupati, Sai

    2016-02-01

    CellLab-CTS 2015 is a Python-language software library for creating two-dimensional, continuous-time stochastic (CTS) cellular automaton models. The model domain consists of a set of grid nodes, with each node assigned an integer state code that represents its condition or composition. Adjacent pairs of nodes may undergo transitions to different states, according to a user-defined average transition rate. A model is created by writing a Python code that defines the possible states, the transitions, and the rates of those transitions. The code instantiates, initializes, and runs one of four object classes that represent different types of CTS models. CellLab-CTS provides the option of using either square or hexagonal grid cells. The software provides the ability to treat particular grid-node states as moving particles, and to track their position over time. Grid nodes may also be assigned user-defined properties, which the user can update after each transition through the use of a callback function. As a component of the Landlab modeling framework, CellLab-CTS models take advantage of a suite of Landlab's tools and capabilities, such as support for standardized input and output.

  13. PEM Fuel Cells from Single Cell to Stack - Fundamental, Modeling, Analysis, and Applications

    OpenAIRE

    Maher A.R. Sadiq Al-Baghdadi

    2015-01-01

    Part I: Fundamentals Chapter 1: Introduction. Chapter 2: PEM fuel cell thermodynamics, electrochemistry, and performance. Chapter 3: PEM fuel cell components. Chapter 4: PEM fuel cell failure modes. Part II: Modeling and Simulation Chapter 5: PEM fuel cell models based on semi-empirical simulation. Chapter 6: PEM fuel cell models based on computational fluid dynamics (CFD). Part III: Analysis Chapter 7: PEM fuel cell analysis. Chapter 8: PEM fuel cell stack desig...

  14. Complementation of the UV-sensitive phenotype of a xeroderma pigmentosum human cell line by transfection with a cDNA clone library

    International Nuclear Information System (INIS)

    In previous work, a xeroderma pigmentosum cell line belonging to complementation group C was established by transformation with origin-defective simian virus 40. We now report the complementation of the UV sensitivity of this cell line by gene transfer. A human cDNA clone library constructed in a mammalian expression vector, and itself incorporated in a lambda phage vector, was introduced into the cells as a calcium phosphate precipitate. Following selection to G418 resistance, provided by the neo gene of the vector, transformants were selected for UV resistance. Twenty-one cell clones were obtained with UV-resistance levels typical of normal human fibroblasts. All transformants contained vector DNA sequences in their nuclei. Upon further propagation in the absence of selection for G418 resistance, about half of the primary transformants remained UV-resistant. Secondary transformants were generated by transfection with a partial digest of total chromosomal DNA from one of these stable transformants. This resulted in 15 G418-resistant clones, 2 of which exhibited a UV-resistant phenotype. The other primary clones lost UV resistance rapidly when subcultured in the absence of G418. Importantly, several retained UV resistance under G418 selection pressure. The acquisition of UV resistance by secondary transformants derived by transfection of DNA from a stable primary transformant, and the linkage between G418 and UV resistances in the unstable primary transformants, strongly suggests that the transformants acquired UV resistance through DNA-mediated gene transfer and not by reversion

  15. Model for decision making about the curricular contents of Knowledge Organization in academic programs of Library Science, Documentation or Information Science

    Directory of Open Access Journals (Sweden)

    Ana María Martínez Tamayo

    2014-04-01

    Full Text Available A model is introduced for decision making on curricular contents of Knowledge Organization for academic programs of Library Science, Documentation and Information Science, orientated to the Mercosur region. The design of the model has taken into account the core contents recommended by the Mercosur meetings of directors and teachers from library schools, the so called ISKO trend, and the contributions of several authors related to both sources. The model has three primary categories: foundations, applications and ethics of Knowledge Organization. The foundations are considered from a multidimensional approach. The applications are divided into three secondary categories: a construction of knowledge organization systems, b content description, and c quality management of Knowledge Organization. The ethics is also divided into three secondary categories: ethical commitment, ethical values and ethical problems. The model should be understood as an empty mold that needs to be filled with the contents determined by each academic program or curricular space, and it is expected that facilitates the assessment, updating and comparison of those contents. The limitation of the model is that it is based on the Mercosur recommendations and ISKO trends, which are not accepted by all the specialists.

  16. Interaction of Defensins with Model Cell Membranes

    Science.gov (United States)

    Sanders, Lori K.; Schmidt, Nathan W.; Yang, Lihua; Mishra, Abhijit; Gordon, Vernita D.; Selsted, Michael E.; Wong, Gerard C. L.

    2009-03-01

    Antimicrobial peptides (AMPs) comprise a key component of innate immunity for a wide range of multicellular organisms. For many AMPs, activity comes from their ability to selectively disrupt and lyse bacterial cell membranes. There are a number of proposed models for this action, but the detailed molecular mechanism of selective membrane permeation remains unclear. Theta defensins are circularized peptides with a high degree of selectivity. We investigate the interaction of model bacterial and eukaryotic cell membranes with theta defensins RTD-1, BTD-7, and compare them to protegrin PG-1, a prototypical AMP, using synchrotron small angle x-ray scattering (SAXS). The relationship between membrane composition and peptide induced changes in membrane curvature and topology is examined. By comparing the membrane phase behavior induced by these different peptides we will discuss the importance of amino acid composition and placement on membrane rearrangement.

  17. A novel method for the line-of-response and time-of-flight reconstruction in TOF-PET detectors based on a library of synchronized model signals

    Energy Technology Data Exchange (ETDEWEB)

    Moskal, P., E-mail: p.moskal@uj.edu.pl [Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 30-348 Cracow (Poland); Zoń, N.; Bednarski, T.; Białas, P.; Czerwiński, E.; Gajos, A.; Kamińska, D. [Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 30-348 Cracow (Poland); Kapłon, Ł. [Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 30-348 Cracow (Poland); Institute of Metallurgy and Materials Science of Polish Academy of Sciences, 30-059 Cracow (Poland); Kochanowski, A. [Faculty of Chemistry, Jagiellonian University, 30-060 Cracow (Poland); Korcyl, G.; Kowal, J. [Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 30-348 Cracow (Poland); Kowalski, P. [Świerk Computing Centre, National Centre for Nuclear Research, 05-400 Otwock-Świerk (Poland); Kozik, T.; Krzemień, W.; Kubicz, E.; Niedźwiecki, Sz.; Pałka, M. [Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 30-348 Cracow (Poland); Raczyński, L. [Świerk Computing Centre, National Centre for Nuclear Research, 05-400 Otwock-Świerk (Poland); Rudy, Z.; Rundel, O. [Faculty of Physics, Astronomy and Applied Computer Science, Jagiellonian University, 30-348 Cracow (Poland); and others

    2015-03-01

    A novel method of hit time and hit position reconstruction in scintillator detectors is described. The method is based on comparison of detector signals with results stored in a library of synchronized model signals registered for a set of well-defined positions of scintillation points. The hit position is reconstructed as the one corresponding to the signal from the library which is most similar to the measurement signal. The time of the interaction is determined as a relative time between the measured signal and the most similar one in the library. A degree of similarity of measured and model signals is defined as the distance between points representing the measurement- and model-signal in the multi-dimensional measurement space. Novelty of the method lies also in the proposed way of synchronization of model signals enabling direct determination of the difference between time-of-flights (TOF) of annihilation quanta from the annihilation point to the detectors. The introduced method was validated using experimental data obtained by means of the double strip prototype of the J-PET detector and {sup 22}Na sodium isotope as a source of annihilation gamma quanta. The detector was built out from plastic scintillator strips with dimensions of 5 mm×19 mm×300 mm, optically connected at both sides to photomultipliers, from which signals were sampled by means of the Serial Data Analyzer. Using the introduced method, the spatial and TOF resolution of about 1.3 cm (σ) and 125 ps (σ) were established, respectively.

  18. A novel method for the line-of-response and time-of-flight reconstruction in TOF-PET detectors based on a library of synchronized model signals

    International Nuclear Information System (INIS)

    A novel method of hit time and hit position reconstruction in scintillator detectors is described. The method is based on comparison of detector signals with results stored in a library of synchronized model signals registered for a set of well-defined positions of scintillation points. The hit position is reconstructed as the one corresponding to the signal from the library which is most similar to the measurement signal. The time of the interaction is determined as a relative time between the measured signal and the most similar one in the library. A degree of similarity of measured and model signals is defined as the distance between points representing the measurement- and model-signal in the multi-dimensional measurement space. Novelty of the method lies also in the proposed way of synchronization of model signals enabling direct determination of the difference between time-of-flights (TOF) of annihilation quanta from the annihilation point to the detectors. The introduced method was validated using experimental data obtained by means of the double strip prototype of the J-PET detector and 22Na sodium isotope as a source of annihilation gamma quanta. The detector was built out from plastic scintillator strips with dimensions of 5 mm×19 mm×300 mm, optically connected at both sides to photomultipliers, from which signals were sampled by means of the Serial Data Analyzer. Using the introduced method, the spatial and TOF resolution of about 1.3 cm (σ) and 125 ps (σ) were established, respectively

  19. Transforming growth factor-β1 phage model peptides isolated from a phage display 7-mer peptide library can inhibit the activity of keloid fibroblasts

    Institute of Scientific and Technical Information of China (English)

    ZONG Xian-lei; JIANG Du-yin; WANG Ji-chang; LIU Jun-li; LIU Zhen-zhong; CAI Jing-long

    2011-01-01

    Background Transforming growth factor-β1 (TGF-β1) is known to have a role in keloid formation through the activation of fibroblasts and the acceleration of collagen deposition. The objective of this current study was to isolate TGF-β1 phage model peptides from a phage display 7-mer peptide library to evaluate their therapeutic effect on inhibiting the activity of keloid fibroblasts.Methods A phage display 7-mer peptide library was screened using monoclonal anti-human TGF-β1 as the target to obtain specific phages containing ectogenous model peptides similar to TGF-β1. Enzyme-linked immunosorbent assay (ELISA) was performed to select monoclonal phages with good binding activity, which underwent DNA sequencing. MTT assay and apoptosis assessment were used to evaluate the biological effects of the phage model peptides on keloid fibroblasts. Immunofluorescence assay was employed to show the binding affinity of the model peptides on phages causing keloid fibroblasts. Quantitative real-time PCR analysis was carried out to detect the expressions of nuclear factor κB (NF-κB) mRNA, connective tissue growth factor (CTGF) mRNA and TGF-β receptor Ⅱ (TβRII) mRNA in keloid fibroblasts.Results Specific phages with good results of ELISA were beneficiated. Four phage model peptides were obtained. The data of MTT showed that TGF-β1 and one phage model peptide (No. 4) could promote keloid fibroblasts proliferation,however, three phage model peptides (No. 1-3) could inhibit keloid fibroblasts proliferation. The results of apoptosis assessment showed that the three phage model peptides could slightly induce the apoptosis in keloid fibroblasts. The data of immunofluorescence assay revealed that the model peptides on phages rather than phages could bind to keloid fibroblasts. The findings of quantitative real-time PCR analysis suggested that the expressions of NF-κB mRNA and CTGF mRNA in the three phage model peptide groups decreased, while the expression of TβRII m

  20. An Annular Plate Model in Arbitrary-Lagrangian-Eulerian Description for the DLR FlexibleBodies Library

    OpenAIRE

    Heckmann, Andreas; Hartweg, Stefan; Kaiser, Ingo

    2011-01-01

    The bending deformation of rotating annular plates and the associated vibration behaviour is important in engineering applications which range from automotive or railway brake systems to discs that form essential components in turbomachinery. In order to extend the capabilities of the DLR FlexibleBodies library for such use cases, a new Modelica class has been implemented which is based on the analytical description of an annular Kirchhoff plate. In addition the so-called Arbitray Langra...

  1. On the Information Service Model of College Library in the Information Age%信息时代高校图书馆信息服务模式

    Institute of Scientific and Technical Information of China (English)

    晁卫华

    2011-01-01

    The change of user's information needs and development of modern information technology in the information age require the library to continuously improve the quality and level of its services to meet user's information needs.The author analyzes from the following 6 aspects in order to improve the quality and level of library services: personalization information service models,disciplinary service models,reference service models,subject information gateway service models and information intelligent agent.%随着网络信息时代的到来,用户信息需求的改变以及现代信息技术的发展,图书馆要不断提高服务质量和水平以满足用户信息需求为主要目标。研究主要包括个性化信息服务模式、学科化服务模式、参考咨询服务模式、学科信息门户服务模式、信息智能代理服务模式和特色数据库建设6种信息服务模式,以期提高高校图书馆信息服务的质量与水平。

  2. Alternate Cluster Generation for Picomolar NGS Libraries

    OpenAIRE

    Huerta, G.; Heckmann, T; Deiderick, H.; Barnette, M.; Hunicke-Smith, S.P.; Podnar, J.W.

    2014-01-01

    While advancements in library preparation techniques now enable NGS library preparation from single cells and nano- to picograms of DNA or RNA, it can still be difficult to reach Illumina's recommended final library concentration of 2 nM for DNA template preparation prior to cluster generation. This can be an acute issue for ChIP-seq, RIP-seq, and LCM-based samples. We have developed a simple and robust alternative method that maximizes number of reads from an Illumina library without having ...

  3. The Library Building Tomorrow.

    Science.gov (United States)

    Waters, Richard L.

    1987-01-01

    Examination of the library of tomorrow speculates about the impact of changes in the functions of government, technology, demographics, lifestyle, and values on the role of the library. A facility for the contemporary public library is described that can both accommodate traditional services and respond to changes in the library's role. (17…

  4. Growing Competition for Libraries.

    Science.gov (United States)

    Gibbons, Susan

    2001-01-01

    Describes the Questia subscription-based online academic digital books library. Highlights include weaknesses of the collection; what college students want from a library; importance of marketing; competition for traditional academic libraries that may help improve library services; and the ability of Questia to overcome barriers and…

  5. The library marketing toolkit

    CERN Document Server

    Potter, Ned

    2012-01-01

    A guide that offers coverage of various elements of library marketing and branding for different sectors including archives and academic, public and special libraries. It is suitable for those who are involved in promoting their library or information service, whether at an academic, public or special library or in archives or records management.

  6. Economics of Academic Libraries.

    Science.gov (United States)

    Baumol, William J.; Marcus, Matityahu

    An analysis is conducted of economic issues pertinent to library planning in higher education in the face of rising costs and diminishing financial support. The individual chapters deal with: 1) growth rates in large university libraries; 2) library costs in colleges and universities; 3) cost trends and long-range plans; 4) library data; and 5) a…

  7. The New Library Professional

    Science.gov (United States)

    Wilder, Stanley

    2007-01-01

    This article discusses what the growing generation gap among library employees mean for academic research libraries and for the profession. Viewed collectively, the members of the under-35 cohort are a harbinger of a new kind of academic library professional, one whose traits bear directly on the ability of libraries to thrive amid the continuing…

  8. Teleporting the library?

    DEFF Research Database (Denmark)

    Heilesen, Simon

    2009-01-01

    In 2007, six Danish public libraries established a virtual library, Info Island DK, in Second Life. This article discusses the library project in terms of design. The design processes include the planning and implementation of the virtual library structure and its equipment, as well as the organi...

  9. Prison Libraries Inside Out.

    Science.gov (United States)

    Singer, Glen

    2000-01-01

    Discussion of prison libraries provides an inside look at the correctional institution environment, prison security concerns, inmate patrons and library use, library collections and services, librarians and staff, the day-to-day operation of a prison library, and future possibilities and needs. (Author/LRW)

  10. Inside Prison Libraries.

    Science.gov (United States)

    Vogel, Brenda; And Others

    1989-01-01

    Issues related to prison libraries are discussed in six articles. Topics covered include the history of American penitentiary ideology; standards for prison libraries; the controversy as to whether prison libraries should serve prisoners or be used as penological tools; and the lack of knowledge about prison libraries within the general library…

  11. Extended Library Hours

    OpenAIRE

    Devarai, Rajashekhar S.; Devarai, Kanyakumari S.

    1997-01-01

    Extension of library hours is useful both for public & LIS professional. Five laws, need for extended hours, problems of extended library hours, implication of extended library hours. ROCLOLIB are the related topics covered in the paper to highlight the importance of extended library hours.

  12. A Planar Mechanical Library for Teaching Modelica

    OpenAIRE

    Zimmer, Dirk

    2012-01-01

    Teaching Modelica to students of a university requires suitable example models. This paper describes a planar mechanical library that is primarily conceived for teaching purposes. It is simple, built out of a few components only but enables the modeling of interesting and complex systems. The library is freely available and supported by various Modelica environments.

  13. 基于SWOT模型的图书馆竞争力分析%Analysis on Library Competitiveness Based on SWOT Model

    Institute of Scientific and Technical Information of China (English)

    陈林

    2016-01-01

    SWOT分析是组织竞争力分析的重要方法,文章利用SWOT模型分析了图书馆发展中存在的机会、优势、劣势和威胁,以期为图书馆创新服务内容、转变服务方式提供有力支持。%The SWOT analysis is an important method to analyze organizations ’ competitiveness. The paper uses the SWOT model to analyze the library’s opportunities, advantages, disadvantages and threats in order to support the library service innovation.

  14. Analysis on Library Competitiveness Based on SWOT Model%基于SWOT模型的图书馆竞争力分析

    Institute of Scientific and Technical Information of China (English)

    陈林

    2016-01-01

    SWOT分析是组织竞争力分析的重要方法,文章利用SWOT模型分析了图书馆发展中存在的机会、优势、劣势和威胁,以期为图书馆创新服务内容、转变服务方式提供有力支持。%The SWOT analysis is an important method to analyze organizations ’ competitiveness. The paper uses the SWOT model to analyze the library’s opportunities, advantages, disadvantages and threats in order to support the library service innovation.

  15. Modeling Anomalous Hysteresis in Perovskite Solar Cells.

    Science.gov (United States)

    van Reenen, Stephan; Kemerink, Martijn; Snaith, Henry J

    2015-10-01

    Organic-inorganic lead halide perovskites are distinct from most other semiconductors because they exhibit characteristics of both electronic and ionic motion. Accurate understanding of the optoelectronic impact of such properties is important to fully optimize devices and be aware of any limitations of perovskite solar cells and broader optoelectronic devices. Here we use a numerical drift-diffusion model to describe device operation of perovskite solar cells. To achieve hysteresis in the modeled current-voltage characteristics, we must include both ion migration and electronic charge traps, serving as recombination centers. Trapped electronic charges recombine with oppositely charged free electronic carriers, of which the density depends on the bias-dependent ion distribution in the perovskite. Our results therefore show that reduction of either the density of mobile ionic species or carrier trapping at the perovskite interface will remove the adverse hysteresis in perovskite solar cells. This gives a clear target for ongoing research effort and unifies previously conflicting experimental observations and theories.

  16. SPLAT: Innovative Collaboration in Idaho's Libraries

    Directory of Open Access Journals (Sweden)

    Amy Vecchione

    2011-01-01

    Full Text Available Libraries face shrinking budgets, increased use, and user demand for trendy resources. This makes it difficult for librarians to find the time to keep current with innovative library trends, such as technological tools and social media developments. The Special Projects Library Action Team (SPLAT offers a new model for enhancing library services. SPLAT is a group supported by the Idaho Commission for Libraries (ICFL, the state agency responsible for assisting libraries. The members of SPLAT are innovation representatives who search and experiment with social media trends and online tools, and share the best ways to integrate them into services at all types of libraries. SPLAT members have developed SPLAT 101, an online class geared towards teaching library staff new Web technologies. Members also present about trends at conferences, blog relevant content, and engage in peer-to-peer education--all meant to demystify and exploit emergent technologies. These efforts have yielded enhanced library services, encouraged changes to policies, and increased positive user experiences. In this article we summarize how SPLAT works, explain how SPLAT has helped Idaho's libraries experiment with evolving services, analyze the success of SPLAT as a model for other states, and discuss future steps.

  17. An internal ribosome entry site (IRES mutant library for tuning expression level of multiple genes in mammalian cells.

    Directory of Open Access Journals (Sweden)

    Esther Y C Koh

    Full Text Available A set of mutated Encephalomyocarditis virus (EMCV internal ribosome entry site (IRES elements with varying strengths is generated by mutating the translation initiation codons of 10(th, 11(th, and 12(th AUG to non-AUG triplets. They are able to control the relative expression of multiple genes over a wide range in mammalian cells in both transient and stable transfections. The relative strength of each IRES mutant remains similar in different mammalian cell lines and is not gene specific. The expressed proteins have correct molecular weights. Optimization of light chain over heavy chain expression by these IRES mutants enhances monoclonal antibody expression level and quality in stable transfections. Uses of this set of IRES mutants can be extended to other applications such as synthetic biology, investigating interactions between proteins and its complexes, cell engineering, multi-subunit protein production, gene therapy, and reprogramming of somatic cells into stem cells.

  18. A Study on the Organization Model of Regional Library%区域图书馆发展组织模式研究

    Institute of Scientific and Technical Information of China (English)

    孙广成

    2015-01-01

    This paper discusses some characteristics of the library organization model of our country at the beginning of new century that are mainly manifested on the regional scale and management system model. It analyzes some typical models and its regional characteristics due to the difference of administrative system of our country and abroad , the unbalanced current situation of development in libraries on various regions.%文章探讨了在新世纪之初我国图书馆的区域发展在组织模式上的一些特点,这些特点主要体现在区域规模和管理体制模式上.经过分析指出,由于我国行政体制与国外的差异和各地图书馆发展的不平衡的现状使图书馆发展组织模式具有区域特色.

  19. A generic simulation cell method for developing extensible, efficient and readable parallel computational models

    Science.gov (United States)

    Honkonen, I.

    2015-03-01

    I present a method for developing extensible and modular computational models without sacrificing serial or parallel performance or source code readability. By using a generic simulation cell method I show that it is possible to combine several distinct computational models to run in the same computational grid without requiring modification of existing code. This is an advantage for the development and testing of, e.g., geoscientific software as each submodel can be developed and tested independently and subsequently used without modification in a more complex coupled program. An implementation of the generic simulation cell method presented here, generic simulation cell class (gensimcell), also includes support for parallel programming by allowing model developers to select which simulation variables of, e.g., a domain-decomposed model to transfer between processes via a Message Passing Interface (MPI) library. This allows the communication strategy of a program to be formalized by explicitly stating which variables must be transferred between processes for the correct functionality of each submodel and the entire program. The generic simulation cell class requires a C++ compiler that supports a version of the language standardized in 2011 (C++11). The code is available at https://github.com/nasailja/gensimcell for everyone to use, study, modify and redistribute; those who do are kindly requested to acknowledge and cite this work.

  20. America's Star Libraries, 2010: Top-Rated Libraries

    Science.gov (United States)

    Lyons, Ray; Lance, Keith Curry

    2010-01-01

    The "LJ" Index of Public Library Service 2010, "Library Journal"'s national rating of public libraries, identifies 258 "star" libraries. Created by Ray Lyons and Keith Curry Lance, and based on 2008 data from the IMLS, it rates 7,407 public libraries. The top libraries in each group get five, four, or three stars. All included libraries, stars or…

  1. Library thermohydraulic components for training simulators

    International Nuclear Information System (INIS)

    The thermohydraulic components Library was modeled in MatLab/Simulink®. This library owns Pipe type components (pump, control valve and / or heaters), storage tanks (Open, Closed and Equilibrium Water Vapor-Air) and Heat Exchangers (Co-Current, Counter-Current and U-tubes). Each component can be attached to other components through the component library Header, in order to create a more complex thermal-hydraulic system which in turn can interact with other thermal-hydraulic systems. (author)

  2. Organic solvent simulations under non-periodic boundary conditions: A library of effective potentials for the GLOB model

    Science.gov (United States)

    Mancini, Giordano; Brancato, Giuseppe; Chandramouli, Balasubramanian; Barone, Vincenzo

    2015-04-01

    We extend the library of solvents that can be treated using the GLOB (general liquid optimized boundary) method, that allows to perform MD simulations under non-periodic boundary conditions (NPBC) optimizing effective potentials between explicit molecules and the boundary for four organic solvents: CHCl3, CCl4, CH3OH and CH3CN. We show that GLOB allows reducing the number of explicit solvent shells to be included, while yielding results comparable with PBC and significant advantages over simulations without explicit boundaries. Finally, we provide polynomial fittings for all available GLOB effective potentials (including SPC water) to simplify their implementation in NPBC MD simulations.

  3. Parallel Libraries to support High-Level Programming

    DEFF Research Database (Denmark)

    Larsen, Morten Nørgaard

    of the more exotic though short-lived heterogeneous CELL Broadband Engine (CELL-BE) architecture added to this shift. Furthermore, the use of cluster computers made of commodity hardware and specialized supercomputers have greatly increased in both industry as well as in the academic world. Finally...... an extension based on Communicating Sequential Processes (CSP) for a distributed shared memory system for the CELL Broadband Engine. This extension consists of a channel model and of a thread library for the CELL’s specialized computational units enabling them to run multiple (CSP) processes. Overall, the CSP...

  4. cDNA isolated from a human T-cell library encodes a member of the protein-tyrosine-phosphatase family

    International Nuclear Information System (INIS)

    A human peripheral T-cell cDNA library was screened with two labeled synthetic oligonucleotides encoding regions of a human placenta protein-tyrosine-phosphatase. One positive clone was isolated and the nucleotide sequence was determined. It contained 1,305 base pairs of open reading frame followed by a TAA stop codon and 978 base pairs of 3' untranslated end, although a poly(A)+ tail was not found. An initiator methionine residue was predicted at position 61, which would result in a protein of 415 amino acid residues. This was supported by the synthesis of a Mr 48,000 protein in an in vitro reticulocyte lysate translation system using RNA transcribed from the cloned cDNA and T7 RNA polymerase. The deduced amino acid sequence was compared to other known proteins revealing 65% identity to the low Mr PTPase 1B isolated from placenta. In view of the high degree of similarity, the T-cell cDNA likely encodes a newly discovered protein-tyrosine-phosphatase, thus expanding this family of genes

  5. Advanced methods of solid oxide fuel cell modeling

    CERN Document Server

    Milewski, Jaroslaw; Santarelli, Massimo; Leone, Pierluigi

    2011-01-01

    Fuel cells are widely regarded as the future of the power and transportation industries. Intensive research in this area now requires new methods of fuel cell operation modeling and cell design. Typical mathematical models are based on the physical process description of fuel cells and require a detailed knowledge of the microscopic properties that govern both chemical and electrochemical reactions. ""Advanced Methods of Solid Oxide Fuel Cell Modeling"" proposes the alternative methodology of generalized artificial neural networks (ANN) solid oxide fuel cell (SOFC) modeling. ""Advanced Methods

  6. Modeling Degradation in Solid Oxide Electrolysis Cells

    Energy Technology Data Exchange (ETDEWEB)

    Manohar S. Sohal; Anil V. Virkar; Sergey N. Rashkeev; Michael V. Glazoff

    2010-09-01

    Idaho National Laboratory has an ongoing project to generate hydrogen from steam using solid oxide electrolysis cells (SOECs). To accomplish this, technical and degradation issues associated with the SOECs will need to be addressed. This report covers various approaches being pursued to model degradation issues in SOECs. An electrochemical model for degradation of SOECs is presented. The model is based on concepts in local thermodynamic equilibrium in systems otherwise in global thermodynamic no equilibrium. It is shown that electronic conduction through the electrolyte, however small, must be taken into account for determining local oxygen chemical potential, , within the electrolyte. The within the electrolyte may lie out of bounds in relation to values at the electrodes in the electrolyzer mode. Under certain conditions, high pressures can develop in the electrolyte just near the oxygen electrode/electrolyte interface, leading to oxygen electrode delamination. These predictions are in accordance with the reported literature on the subject. Development of high pressures may be avoided by introducing some electronic conduction in the electrolyte. By combining equilibrium thermodynamics, no equilibrium (diffusion) modeling, and first-principles, atomic scale calculations were performed to understand the degradation mechanisms and provide practical recommendations on how to inhibit and/or completely mitigate them.

  7. Cell culture models for study of differentiated adipose cells

    OpenAIRE

    Clynes, Martin

    2014-01-01

    Adipose cells are an important source of mesenchymal stem cells and are important for direct use in research on lipid metabolism and obesity. In addition to use of primary cultures, there is increasing interest in other sources of larger numbers of cells, using approaches including induced pluripotent stem cell differentiation and viral immortalisation.

  8. Academic library use of ebooks

    CERN Document Server

    2014-01-01

    This 165-page international study looks closely at how academic libraries are using eBooks. It helps librarians to answer questions such as: how fast is eBook spending growing? Which eBook purchasing models are growing fastest? Subscription models that allow unlimited access” pay per view models? Outright ownership? Other models? What percentage of eBook purchases are through consortia? What percentage direct from publishers? What percentage through aggregators?

  9. The Application of Library Crowdsourcing Model in Information Education%众包在大学生信息素养教育中的应用

    Institute of Scientific and Technical Information of China (English)

    肖检蓉

    2011-01-01

    我国大学生的信息素养现状距信息时代的能力要求还有较大差距。主要原因之一就是现有的信息素养教育模式不能很好地适应人才培养要求。众包作为一种全新的理念和运作模式,成为支持网络时代图书馆创新的有效途径。通过总结信息素养教育的主要教学形式,以新生入馆教育为例,对众包模式的组织与管理进行了实践探索。%The main reason that there exists ucation and the requirement of the information a comparatively big gap in our country between the information ed- era is that the current information education model cannot perfectly meet the need of the innovative talents training, which must be changed through exploring a new education model. As a new idea and performance model, crowdsourcing will be an effective road to support library innovation at the net- work time. By analyzing main teaching models in information education, the paper provides a practical exploration on the organization and administration of crowdsourcing model through the example of freshmen's education on accessing to the library of universities.

  10. A Physics-based Analytical Model for Perovskite Solar Cells

    OpenAIRE

    Sun, Xingshu; Asadpour, Reza; Nie, Wanyi; Mohite, Aditya D.; Alam, Muhammad A.

    2015-01-01

    Perovskites are promising next-generation absorber materials for low-cost and high-efficiency solar cells. Although perovskite cells are configured similar to the classical solar cells, their operation is unique and requires development of a new physical model for characterization, optimization of the cells, and prediction of the panel performance. In this paper, we develop such a physics-based analytical model to describe the operation of different types of perovskite solar cells, explicitly...

  11. Modelling familial dysautonomia in human induced pluripotent stem cells

    OpenAIRE

    Lee, Gabsang; Studer, Lorenz

    2011-01-01

    Induced pluripotent stem (iPS) cells have considerable promise as a novel tool for modelling human disease and for drug discovery. While the generation of disease-specific iPS cells has become routine, realizing the potential of iPS cells in disease modelling poses challenges at multiple fronts. Such challenges include selecting a suitable disease target, directing the fate of iPS cells into symptom-relevant cell populations, identifying disease-related phenotypes and showing reversibility of...

  12. Mouse models for cancer stem cell research

    OpenAIRE

    Cheng, Le; Ramesh, Anirudh V.; Flesken-Nikitin, Andrea; Choi, Jinhyang; Nikitin, Alexander Yu.

    2009-01-01

    Cancer stem cell concept assumes that cancers are mainly sustained by a small pool of neoplastic cells, known as cancer stem cells or tumor initiating cells, which are able to reproduce themselves and produce phenotypically heterogeneous cells with lesser tumorigenic potential. Cancer stem cells represent an appealing target for development of more selective and efficient therapies. However, direct testing of the cancer stem cell concept and assessment of its therapeutic implications in human...

  13. Updated Core Libraries of the ALPS Project

    CERN Document Server

    Gaenko, A; Carcassi, G; Chen, T; Chen, X; Dong, Q; Gamper, L; Gukelberger, J; Igarashi, R; Iskakov, S; Könz, M; LeBlanc, J P F; Levy, R; Ma, P N; Paki, J E; Shinaoka, H; Todo, S; Troyer, M; Gull, E

    2016-01-01

    The open source ALPS (Algorithms and Libraries for Physics Simulations) project provides a collection of physics libraries and applications, with a focus on simulations of lattice models and strongly correlated systems. The libraries provide a convenient set of well-documented and reusable components for developing condensed matter physics simulation code, and the applications strive to make commonly used and proven computational algorithms available to a non-expert community. In this paper we present an updated and refactored version of the core ALPS libraries geared at the computational physics software development community, rewritten with focus on documentation, ease of installation, and software maintainability.

  14. Training library patrons the ADDIE way

    CERN Document Server

    Wegener, Debby

    2006-01-01

    This book takes an informal and in-depth look at the five steps of the ADDIE model - Analysis, Design, Development, Implementation and Evaluation - as used in library training programmes. With hints and tips and practical advice from other trainers, the aims of the book are to (1) make the training of library patrons a simple task and (2) to show library staff that training can be rewarding and extremely satisfying. Anyone who has had to design a library training programme will know that it can be quite a daunting task without guidelines, but when it comes to the various learning theories and

  15. Introduction to the library and information professions

    CERN Document Server

    Greer, Roger; Fowler, Susan

    2013-01-01

    In this rapidly changing, knowledge-based society, library and information professionals require a broad understanding of the profession. Introduction to the Library and Information Professions, Second Edition presents a toolbox of models that enable this essential understanding for undergraduate and graduate students in library and information science programs as well as practicing professionals seeking continuing education. The materials in this second edition reflect the latest trends in the library and information profession, including services and issues that stem from new advances in te

  16. Biomechanics of epithelial cell islands analyzed by modeling and experimentation

    CERN Document Server

    Coburn, Luke; Noppe, Adrian; Caldwell, Benjamin J; Moussa, Elliott; Yap, Chloe; Priya, Rashmi; Lobaskin, Vladimir; Roberts, Anthony P; Yap, Alpha S; Neufeld, Zoltan; Gomez, Guillermo A

    2016-01-01

    We generated a new computational approach to analyze the biomechanics of epithelial cell islands that combines both vertex and contact-inhibition-of-locomotion models to include both cell-cell and cell-substrate adhesion. Examination of the distribution of cell protrusions (adhesion to the substrate) in the model predicted high order profiles of cell organization that agree with those previously seen experimentally. Cells acquired an asymmetric distribution of protrusions (and traction forces) that decreased when moving from the edge to the island center. Our in silico analysis also showed that tension on cell-cell junctions (and monolayer stress) is not homogeneous across the island. Instead it is higher at the island center and scales up with island size, which we confirmed experimentally using laser ablation assays and immunofluorescence. Moreover, our approach has the minimal elements necessary to reproduce mechanical crosstalk between both cell-cell and cell substrate adhesion systems. We found that an i...

  17. Computational cell model based on autonomous cell movement regulated by cell-cell signalling successfully recapitulates the "inside and outside" pattern of cell sorting

    Directory of Open Access Journals (Sweden)

    Ajioka Itsuki

    2007-09-01

    Full Text Available Abstract Background Development of multicellular organisms proceeds from a single fertilized egg as the combined effect of countless numbers of cellular interactions among highly dynamic cells. Since at least a reminiscent pattern of morphogenesis can be recapitulated in a reproducible manner in reaggregation cultures of dissociated embryonic cells, which is known as cell sorting, the cells themselves must possess some autonomous cell behaviors that assure specific and reproducible self-organization. Understanding of this self-organized dynamics of heterogeneous cell population seems to require some novel approaches so that the approaches bridge a gap between molecular events and morphogenesis in developmental and cell biology. A conceptual cell model in a computer may answer that purpose. We constructed a dynamical cell model based on autonomous cell behaviors, including cell shape, growth, division, adhesion, transformation, and motility as well as cell-cell signaling. The model gives some insights about what cellular behaviors make an appropriate global pattern of the cell population. Results We applied the model to "inside and outside" pattern of cell-sorting, in which two different embryonic cell types within a randomly mixed aggregate are sorted so that one cell type tends to gather in the central region of the aggregate and the other cell type surrounds the first cell type. Our model can modify the above cell behaviors by varying parameters related to them. We explored various parameter sets with which the "inside and outside" pattern could be achieved. The simulation results suggested that direction of cell movement responding to its neighborhood and the cell's mobility are important for this specific rearrangement. Conclusion We constructed an in silico cell model that mimics autonomous cell behaviors and applied it to cell sorting, which is a simple and appropriate phenomenon exhibiting self-organization of cell population. The model

  18. A sub-cellular viscoelastic model for cell population mechanics.

    Directory of Open Access Journals (Sweden)

    Yousef Jamali

    Full Text Available Understanding the biomechanical properties and the effect of biomechanical force on epithelial cells is key to understanding how epithelial cells form uniquely shaped structures in two or three-dimensional space. Nevertheless, with the limitations and challenges posed by biological experiments at this scale, it becomes advantageous to use mathematical and 'in silico' (computational models as an alternate solution. This paper introduces a single-cell-based model representing the cross section of a typical tissue. Each cell in this model is an individual unit containing several sub-cellular elements, such as the elastic plasma membrane, enclosed viscoelastic elements that play the role of cytoskeleton, and the viscoelastic elements of the cell nucleus. The cell membrane is divided into segments where each segment (or point incorporates the cell's interaction and communication with other cells and its environment. The model is capable of simulating how cells cooperate and contribute to the overall structure and function of a particular tissue; it mimics many aspects of cellular behavior such as cell growth, division, apoptosis and polarization. The model allows for investigation of the biomechanical properties of cells, cell-cell interactions, effect of environment on cellular clusters, and how individual cells work together and contribute to the structure and function of a particular tissue. To evaluate the current approach in modeling different topologies of growing tissues in distinct biochemical conditions of the surrounding media, we model several key cellular phenomena, namely monolayer cell culture, effects of adhesion intensity, growth of epithelial cell through interaction with extra-cellular matrix (ECM, effects of a gap in the ECM, tensegrity and tissue morphogenesis and formation of hollow epithelial acini. The proposed computational model enables one to isolate the effects of biomechanical properties of individual cells and the

  19. Application of Cloud Technology in Digital Library

    Directory of Open Access Journals (Sweden)

    D Kishore Kumar

    2012-05-01

    Full Text Available Libraries may soon be building and managing their own data centers. This model would letlibraries maintain more control over the applications and data stores that containsensitive, private information about patrons. Provisioning and maintenance of infrastructure forWeb based digital library present several challenges. In this paper we discuss problems facedwith digital library and development efforts to overcome that problem. Infrastructure virtualizationand cloud computing are particularly attractive choices which is challenged by both growth inthe size of the indexed document collection, new features and most prominently usage. With thepurpose of applying Cloud Computing to university library, the paper describes the currentstatus of user service models in university libraries. Then it proposed to improve current userservice model with Cloud Computing. This paper explores some of the security issuessurrounding data location, mobility and availability.

  20. Homogenization of a viscoelastic model for plant cell wall biomechanics

    OpenAIRE

    Ptashnyk, Mariya; Seguin, Brian

    2015-01-01

    The microscopic structure of a plant cell wall is given by cellulose microfibrils embedded in a cell wall matrix. In this paper we consider a microscopic model for interactions between viscoelastic deformations of a plant cell wall and chemical processes in the cell wall matrix. We consider elastic deformations of the cell wall microfibrils and viscoelastic Kelvin--Voigt type deformations of the cell wall matrix. Using homogenization techniques (two-scale convergence and periodic unfolding me...

  1. Computational Modeling of T Cell Receptor Complexes.

    Science.gov (United States)

    Riley, Timothy P; Singh, Nishant K; Pierce, Brian G; Weng, Zhiping; Baker, Brian M

    2016-01-01

    T-cell receptor (TCR) binding to peptide/MHC determines specificity and initiates signaling in antigen-specific cellular immune responses. Structures of TCR-pMHC complexes have provided enormous insight to cellular immune functions, permitted a rational understanding of processes such as pathogen escape, and led to the development of novel approaches for the design of vaccines and other therapeutics. As production, crystallization, and structure determination of TCR-pMHC complexes can be challenging, there is considerable interest in modeling new complexes. Here we describe a rapid approach to TCR-pMHC modeling that takes advantage of structural features conserved in known complexes, such as the restricted TCR binding site and the generally conserved diagonal docking mode. The approach relies on the powerful Rosetta suite and is implemented using the PyRosetta scripting environment. We show how the approach can recapitulate changes in TCR binding angles and other structural details, and highlight areas where careful evaluation of parameters is needed and alternative choices might be made. As TCRs are highly sensitive to subtle structural perturbations, there is room for improvement. Our method nonetheless generates high-quality models that can be foundational for structure-based hypotheses regarding TCR recognition. PMID:27094300

  2. The Marketing of Public Library Services.

    Science.gov (United States)

    Dragon, Andrea C.

    1983-01-01

    Defines the concept of marketing and relates models involving the exchanges and transactions of markets and charities to services offered by libraries. Market segmentation, understanding the behavior of markets, competition, and movement toward a market-oriented library are highlighted. Nineteen references are cited. (EJS)

  3. Librarians and Libraries Supporting Open Access Publishing

    Science.gov (United States)

    Richard, Jennifer; Koufogiannakis, Denise; Ryan, Pam

    2009-01-01

    As new models of scholarly communication emerge, librarians and libraries have responded by developing and supporting new methods of storing and providing access to information and by creating new publishing support services. This article will examine the roles of libraries and librarians in developing and supporting open access publishing…

  4. Proceedings of the NETL Workshop on Fuel Cell Modeling

    Energy Technology Data Exchange (ETDEWEB)

    Randall S. Gemmen; J. R. Selman

    2000-04-18

    This workshop was the first U.S. DOE sponsored meeting devoted to fuel cell modeling. The workshop was attended by over 45 people from industry, universities, and the government. The goals of the meeting were to assess the status of fuel cell modeling, and determine how new developments in fuel cell modeling can improve cell design, stack design, and power system design. The primary focus was on cell and stack modeling. Following a review of DOE/NETL fuel cell related programs and activities, Professor Robert Selman (Illinois Institute of Technology) kicked off the technical portion of the workshop by presenting an overview of fuel cell phenomena and the status of fuel cell modeling. This overview provided the necessary background for establishing a common framework for discussing fuel cell modeling. A distinction was made between micro modeling, electrode modeling, cell modeling, stack modeling, and system modeling. It was proposed that all modeling levels be supported for further development. In addition, due to significant advances being made outside the U.S., it was proposed that dialog/exchange with other international researchers be established. Following the Overview Session, eight leading researchers in modeling gave individual presentations. These presentations provided additional information on the status and present direction of model developments. All these presentations can be found in Attachment A. Before the workshop, a survey was sent out requesting comments from the attendees. Results from this survey can be found in Attachment B. This survey was then used as initial talking points at the individual breakout sessions on the afternoon of the workshop. Breakouts were organized by microfundamental modeling, cell modeling, stack modeling, and systems modeling.

  5. Correctional Libraries, Library Standards, and Diversity.

    Science.gov (United States)

    Shirley, Glennor L.

    2003-01-01

    Respondents to a survey of prison librarians (n=35) regarding how they cope with demands for materials and services related to diversity issues felt that adherence to Library Standards for Adult Correctional Institutions was often impeded by security concerns. Racial differences between library providers and prisoners was not considered relevant.…

  6. 高校图书馆战略管理模型的构建%The Construction of University Library 's Strategic Management Model

    Institute of Scientific and Technical Information of China (English)

    李情

    2015-01-01

    利用定性方法,设计了高校图书馆管理战略模型,模型包括战略制定、战略实施及战略评价与控制3个基本组件,通过前期、中期、后期3个连续规划阶段的实施,确保这些阶段的最佳发展和执行,并通过核准、质量控制、沟通与参与这3个连接渠道把3个组件连成一个整体,旨在为高校图书馆克服在未来策略中可能面临的障碍提供指导.%Using qualitative analytic method, this paper designs university library's strategic management model, which includes three basic components of strategic formulation, strategic implementation and strategic evaluation and control, and through the implementation of the three consecutive planning stages (early, middle and late stages), ensures the best development and execution of these stages, and connects the three components into a whole through three connecting channels of the approval, quality control and communication and participation in order to provide the guidance for university library to overcome the obstacles that may be faced in the future strategy.

  7. Metacognitive Model of Tacit Knowledge Transfer in Human Library%真人图书馆隐性知识转移的元认知模式

    Institute of Scientific and Technical Information of China (English)

    王培林

    2016-01-01

    To find a solution for“how to transfer tacit knowledge in human library”,this article combines Flavell’ s metacognitive theory with Dr. Dong’s metacognitive ideas to explore the metacognitive model of tacit knowledge transfer in human library and its operating mechanism based on the analysis of metacognitive dimensions of tacit knowledge. The results show that it need to establish a “3+1” metacognition mechanism for tacit knowledge transfer in human library in order to achieve the transfer successfully.%文章针对真人图书馆中隐性知识如何转移,引入Flavell的元认知理论及董奇的元认知观点,在分析隐性知识元认知维度的基础上,探索真人图书馆隐性知识转移的元认知模式,据此提出推动真人图书馆隐性知识转移的运行机制。研究结果显示,要成功实现真人图书馆隐性知识转移,需要建立“3+1”真人图书馆隐性知识转移元认知机制。

  8. 'Digital Library: A New Concept of Library

    OpenAIRE

    Deshmukh A. Hadi A. Bari

    2012-01-01

    This article throws light on introduction of Digital Library, Definition of Digital Library, Need, Objective, Function, Concept; Advantages & Disadvantages of Digital Library and it also describe components of Digital Library.

  9. Modeling keratinocyte wound healing dynamics: Cell-cell adhesion promotes sustained collective migration.

    Science.gov (United States)

    Nardini, John T; Chapnick, Douglas A; Liu, Xuedong; Bortz, David M

    2016-07-01

    The in vitro migration of keratinocyte cell sheets displays behavioral and biochemical similarities to the in vivo wound healing response of keratinocytes in animal model systems. In both cases, ligand-dependent Epidermal Growth Factor Receptor (EGFR) activation is sufficient to elicit collective cell migration into the wound. Previous mathematical modeling studies of in vitro wound healing assays assume that physical connections between cells have a hindering effect on cell migration, but biological literature suggests a more complicated story. By combining mathematical modeling and experimental observations of collectively migrating sheets of keratinocytes, we investigate the role of cell-cell adhesion during in vitro keratinocyte wound healing assays. We develop and compare two nonlinear diffusion models of the wound healing process in which cell-cell adhesion either hinders or promotes migration. Both models can accurately fit the leading edge propagation of cell sheets during wound healing when using a time-dependent rate of cell-cell adhesion strength. The model that assumes a positive role of cell-cell adhesion on migration, however, is robust to changes in the leading edge definition and yields a qualitatively accurate density profile. Using RNAi for the critical adherens junction protein, α-catenin, we demonstrate that cell sheets with wild type cell-cell adhesion expression maintain migration into the wound longer than cell sheets with decreased cell-cell adhesion expression, which fails to exhibit collective migration. Our modeling and experimental data thus suggest that cell-cell adhesion promotes sustained migration as cells pull neighboring cells into the wound during wound healing. PMID:27105673

  10. Modeling keratinocyte wound healing dynamics: Cell-cell adhesion promotes sustained collective migration.

    Science.gov (United States)

    Nardini, John T; Chapnick, Douglas A; Liu, Xuedong; Bortz, David M

    2016-07-01

    The in vitro migration of keratinocyte cell sheets displays behavioral and biochemical similarities to the in vivo wound healing response of keratinocytes in animal model systems. In both cases, ligand-dependent Epidermal Growth Factor Receptor (EGFR) activation is sufficient to elicit collective cell migration into the wound. Previous mathematical modeling studies of in vitro wound healing assays assume that physical connections between cells have a hindering effect on cell migration, but biological literature suggests a more complicated story. By combining mathematical modeling and experimental observations of collectively migrating sheets of keratinocytes, we investigate the role of cell-cell adhesion during in vitro keratinocyte wound healing assays. We develop and compare two nonlinear diffusion models of the wound healing process in which cell-cell adhesion either hinders or promotes migration. Both models can accurately fit the leading edge propagation of cell sheets during wound healing when using a time-dependent rate of cell-cell adhesion strength. The model that assumes a positive role of cell-cell adhesion on migration, however, is robust to changes in the leading edge definition and yields a qualitatively accurate density profile. Using RNAi for the critical adherens junction protein, α-catenin, we demonstrate that cell sheets with wild type cell-cell adhesion expression maintain migration into the wound longer than cell sheets with decreased cell-cell adhesion expression, which fails to exhibit collective migration. Our modeling and experimental data thus suggest that cell-cell adhesion promotes sustained migration as cells pull neighboring cells into the wound during wound healing.

  11. Modelling electrolyte conductivity in a water electrolyzer cell

    DEFF Research Database (Denmark)

    Caspersen, Michael; Kirkegaard, Julius Bier

    2012-01-01

    An analytical model describing the hydrogen gas evolution under natural convection in an electrolyzer cell is developed. Main purpose of the model is to investigate the electrolyte conductivity through the cell under various conditions. Cell conductivity is calculated from a parallel resistor...... for electrolyte conductivity from combinations of pressure, current density and electrolyte width among others....

  12. NIAID Biodefense Image Library

    Science.gov (United States)

    ... Content Marketing Share this: Main Content Area Image Library These high-resolution (300 dpi) images may be ... for Disease Control and Prevention public health image library . Smallpox Vaccination Study-1 Smallpox vaccination dilution study: ...

  13. National Library of Medicine

    Science.gov (United States)

    ... ideas about NLM's third century, the future of big data, and the role of libraries in supporting research ... ideas about NLM's third century, the future of big data, and the role of libraries in supporting research ...

  14. Libraries - LIBRARIES_ISL_IN: Libraries in Indiana (Indiana State Library, Point Shapefile)

    Data.gov (United States)

    NSGIC GIS Inventory (aka Ramona) — LIBRARIES_ISL_IN is a point shapefile showing the locations of 528 libraries included in Microsoft Excel spreadsheets that are downloadable from the Web page named...

  15. Reutilizing Existing Library Space.

    Science.gov (United States)

    Davis, Marlys Cresap

    1987-01-01

    This discussion of the reutilization of existing library space reviews the decision process and considerations for implementation. Two case studies of small public libraries which reassigned space to better use are provided, including floor plans. (1 reference) (MES)

  16. Facility Focus: Libraries.

    Science.gov (United States)

    College Planning & Management, 2003

    2003-01-01

    Describes the designs of the Ferris State University Library for Information, Technology and Education (FLITE), and the Meyer Library and Information Technology Center at Southwest Missouri State University. Includes photographs. (EV)

  17. Libraries serving dialogue

    CERN Document Server

    Dupont, Odile

    2014-01-01

    This book based on experiences of libraries serving interreligious dialogue, presents themes like library tools serving dialogue between cultures, collections dialoguing, children and young adults dialoguing beyond borders, story telling as dialog, librarians serving interreligious dialogue.

  18. Validation of noise models for single-cell transcriptomics

    NARCIS (Netherlands)

    Grün, Dominic; Kester, Lennart; van Oudenaarden, Alexander

    2014-01-01

    Single-cell transcriptomics has recently emerged as a powerful technology to explore gene expression heterogeneity among single cells. Here we identify two major sources of technical variability: sampling noise and global cell-to-cell variation in sequencing efficiency. We propose noise models to co

  19. Library staff development course.

    OpenAIRE

    Eaton, E K

    1981-01-01

    The Moody Medical Library at the University of Texas Medical Branch plans, presents, and evaluates regularly a staff development program for its employees, including librarians and clerical and technical staff. The program's purpose is to provide continuing education for the library staff while concurrently: (1) providing information concerning specific library services and programs; (2) illustrating the interrelationship of the departments and divisions within the library; (3) developing a s...

  20. Process modeling of fuel cell vehicle power system

    Institute of Scientific and Technical Information of China (English)

    CHEN LiMing; LIN ZhaoJia; MA ZiFeng

    2009-01-01

    Constructed here is a mathematic model of PEM Fuel Cell Vehicle Power System which is composed of fuel supply model, fuel cell stack model and water-heat management model. The model was developed by Matiab/Simulink to evaluate how the major operating variables affect the output performances. Itshows that the constructed model can represent characteristics of the power system closely by comparing modeling results with experimental data, and it can be used in the study and design of fuel cell vehicle power system.

  1. Construction of cDNA library of the treated Changliver cell and quality analysis%常氏肝癌细胞cDNA文库的构建及质量分析

    Institute of Scientific and Technical Information of China (English)

    林俊堂; 王聪睿; 张会勇; 李玉昌; 徐存拴

    2004-01-01

    目的利用RNA转录5′末端转换机制(SMART)构建适合免疫筛选的经去分化培养基处理的常氏肝癌细胞cDNA文库.方法通过反转录PCR和长距离PCR获得常氏肝癌细胞的全长cDNA,然后利用SMART cDNA文库构建试剂盒建立经去分化培养基处理的常氏肝癌细胞cDNA文库.结果通过测定,高质量的包含常氏肝癌细胞全长cDNA的cDNA文库得到建立,扩增cDNA文库的滴度高达4.5 × 1010 pfu*ml-1,重组子内平均插入外源基因片段长度在200 bp到4000 bp之间,约1500 bp左右,并且此文库适合免疫筛选.结论结果显示所构建的经去分化培养基处理的常氏肝癌细胞cDNA文库具有很高的质量, 为进一步研究常氏肝癌细胞和筛选相关基因获得cDNA全长奠定了坚实的基础.%Objective To construct cDNA library of the treated Changliver cell by switching mechanism at 5′ end of RNA transcript (SMART) technique and analyze its quality.Methods cDNA of Changliver cell was aquired with reverse transcription polymerase chain reaction (RT-PCR) and long-distance PCR (LD-PCR),then the cDNA library was constructed with SMART cDNA library construction kit.Results Through testing,the high quality cDNA library containing full length cDNA of Changliver cell had been constructed.The titer of the amplified cDNA library was 4.5 × 1010 pfu*ml-1 and the average exogenous inserts of the recombinants was 1.5 kb.Conclusion These results suggest that the Changliver cell cDNA library has a high quality and lays a solid foundation for researching on Changliver cell and screening

  2. The Library Lobby

    Science.gov (United States)

    Ralston, Anthony

    1971-01-01

    The library is examined as a university-wide resource and as a research facility. The use of the library relative to computing facilities is also considered as is the cost and value of library usage in relation to computer usage. (Author)

  3. PAL: Positional Astronomy Library

    Science.gov (United States)

    Jenness, T.; Berry, D. S.

    2016-06-01

    The PAL library is a partial re-implementation of Pat Wallace's popular SLALIB library written in C using a Gnu GPL license and layered on top of the IAU's SOFA library (or the BSD-licensed ERFA) where appropriate. PAL attempts to stick to the SLA C API where possible.

  4. Body Basics Library

    Science.gov (United States)

    ... a Friend Who Cuts? About the Body Basics Library KidsHealth > For Teens > About the Body Basics Library Print A A A Text Size Did you ... system, part, and process works. Use this medical library to find out about basic human anatomy, how ...

  5. Public Library Finance.

    Science.gov (United States)

    Mason, Marilyn Gell

    This study reviews trends in public library finance; examines recent political, economic, and technological changes; and assesses the impact of these changes on public library services. A history of the public library in America is presented, as well as an analysis of the principles of economics and public finance which reveals that current…

  6. Supervision in Libraries.

    Science.gov (United States)

    Bailey, Martha J.

    Although the literature of library administration draws extensively on that of business management, it is difficult to compare library supervision to business or industrial supervision. Library supervisors often do not have managerial training and may consider their management role as secondary. The educational level of the staff they supervise…

  7. A Truly Bookless Library

    Science.gov (United States)

    Kolowich, Steve

    2011-01-01

    The difference between the University of Texas at San Antonio's Applied Engineering and Technology Library and other science-focused libraries is not that its on-site collection is also available electronically. It is that its on-site collection is only available electronically. The idea of libraries with no bound books has been a recurring theme…

  8. Changing State Digital Libraries

    Science.gov (United States)

    Pappas, Marjorie L.

    2006-01-01

    Research has shown that state virtual or digital libraries are evolving into websites that are loaded with free resources, subscription databases, and instructional tools. In this article, the author explores these evolving libraries based on the following questions: (1) How user-friendly are the state digital libraries?; (2) How do state digital…

  9. Learning Boost C++ libraries

    CERN Document Server

    Mukherjee, Arindam

    2015-01-01

    If you are a C++ programmer who has never used Boost libraries before, this book will get you up-to-speed with using them. Whether you are developing new C++ software or maintaining existing code written using Boost libraries, this hands-on introduction will help you decide on the right library and techniques to solve your practical programming problems.

  10. The Electronic, Eclectic Library.

    Science.gov (United States)

    Dowlin, Kenneth E.

    1980-01-01

    Utilization of telecommunications and computer technology to increase access to information is identified as a primary goal for public libraries. To further service in the future, libraries should consider database services, online access to library resources, online community conferencing, community databases, network resource sharing,…

  11. School Libraries and Innovation

    Science.gov (United States)

    McGrath, Kevin G.

    2015-01-01

    School library programs have measured success by improved test scores. But how do next-generation school libraries demonstrate success as they strive to be centers of innovation and creativity? These libraries offer solutions for school leaders who struggle to restructure existing systems built around traditional silos of learning (subjects and…

  12. Spanish Museum Libraries Network.

    Science.gov (United States)

    Lopez de Prado, Rosario

    This paper describes the creation of an automated network of museum libraries in Spain. The only way in which the specialized libraries in the world today can continue to be active and to offer valid information is to automate the service they offer, and create network libraries with cooperative plans. The network can be configured with different…

  13. Marketing Academic Libraries

    Science.gov (United States)

    Mallon, Melissa, Ed.

    2013-01-01

    Ask any academic librarian if marketing their library and its services is an important task, and the answer will most likely be a resounding "yes!" Particularly in economically troubled times, librarians are increasingly called upon to promote their services and defend their library's worth. Since few academic libraries have in-house marketing…

  14. Analytical Planning for University Libraries.

    Science.gov (United States)

    Leimkuhler, Ferdinand F.; Cooper, Michael D.

    A survey is made of the more important technological and managerial problems in the planning of university library services and recommendations are made for a positive program of innovation and development. Two approaches are explored in considerable detail. The first is the use of operations research models of the acquisition and storage…

  15. Cell sources for in vitro human liver cell culture models.

    Science.gov (United States)

    Zeilinger, Katrin; Freyer, Nora; Damm, Georg; Seehofer, Daniel; Knöspel, Fanny

    2016-09-01

    In vitro liver cell culture models are gaining increasing importance in pharmacological and toxicological research. The source of cells used is critical for the relevance and the predictive value of such models. Primary human hepatocytes (PHH) are currently considered to be the gold standard for hepatic in vitro culture models, since they directly reflect the specific metabolism and functionality of the human liver; however, the scarcity and difficult logistics of PHH have driven researchers to explore alternative cell sources, including liver cell lines and pluripotent stem cells. Liver cell lines generated from hepatomas or by genetic manipulation are widely used due to their good availability, but they are generally altered in certain metabolic functions. For the past few years, adult and pluripotent stem cells have been attracting increasing attention, due their ability to proliferate and to differentiate into hepatocyte-like cells in vitro However, controlling the differentiation of these cells is still a challenge. This review gives an overview of the major human cell sources under investigation for in vitro liver cell culture models, including primary human liver cells, liver cell lines, and stem cells. The promises and challenges of different cell types are discussed with a focus on the complex 2D and 3D culture approaches under investigation for improving liver cell functionality in vitro Finally, the specific application options of individual cell sources in pharmacological research or disease modeling are described. PMID:27385595

  16. Extinction Models for Cancer Stem Cell Therapy

    OpenAIRE

    Sehl, Mary; Zhou, Hua; Sinsheimer, Janet ,; Lange, Kenneth

    2009-01-01

    Cells with stem cell-like properties are now viewed as initiating and sustaining many cancers. This suggests that cancer can be cured by driving these cancer stem cells to extinction. The problem with this strategy is that ordinary stem cells are apt to be killed in the process. This paper sets bounds on the killing differential (difference between death rates of cancer stem cells and normal stem cells) that must exist for the survival of an adequate number of normal stem cells. Our main tool...

  17. A minimal model for spontaneous cell polarization and edge activity in oscillating, rotating and migrating cells

    CERN Document Server

    Raynaud, Franck; Gabella, Chiara; Bornert, Alicia; Sbalzarini, Ivo F; Meister, Jean-Jacques; Verkhovsky, Alexander B

    2016-01-01

    How the cells break symmetry and organize their edge activity to move directionally is a fun- damental question in cell biology. Physical models of cell motility commonly rely on gradients of regulatory factors and/or feedback from the motion itself to describe polarization of edge activity. Theses approaches, however, fail to explain cell behavior prior to the onset of polarization. Our analysis using the model system of polarizing and moving fish epidermal keratocytes suggests a novel and simple principle of self-organization of cell activity in which local cell-edge dynamics depends on the distance from the cell center, but not on the orientation with respect to the front-back axis. We validate this principle with a stochastic model that faithfully reproduces a range of cell-migration behaviors. Our findings indicate that spontaneous polarization, persistent motion, and cell shape are emergent properties of the local cell-edge dynamics controlled by the distance from the cell center.

  18. A transient fuel cell model to simulate HTPEM fuel cell impedance spectra

    DEFF Research Database (Denmark)

    Vang, Jakob Rabjerg; Andreasen, Søren Juhl; Kær, Søren Knudsen

    2011-01-01

    This paper presents a spatially resolved transient fuel cell model applied to the simulation of high temperature PEM fuel cell impedance spectra. The model is developed using a 2D finite volume method approach. The model is resolved along the channel and across the membrane. The model considers...

  19. A new level set model for cell image segmentation

    Science.gov (United States)

    Ma, Jing-Feng; Hou, Kai; Bao, Shang-Lian; Chen, Chun

    2011-02-01

    In this paper we first determine three phases of cell images: background, cytoplasm and nucleolus according to the general physical characteristics of cell images, and then develop a variational model, based on these characteristics, to segment nucleolus and cytoplasm from their relatively complicated backgrounds. In the meantime, the preprocessing obtained information of cell images using the OTSU algorithm is used to initialize the level set function in the model, which can speed up the segmentation and present satisfactory results in cell image processing.

  20. A new level set model for cell image segmentation

    Institute of Scientific and Technical Information of China (English)

    Ma Jing-Feng; Hou Kai; Bao Shang-Lian; Chen Chun

    2011-01-01

    In this paper we first determine three phases of cell images: background, cytoplasm and nucleolus according to the general physical characteristics of cell images, and then develop a variational model, based on these characteristics, to segment nucleolus and cytoplasm from their relatively complicated backgrounds. In the meantime, the preprocessing obtained information of cell images using the OTSU algorithm is used to initialize the level set function in the model, which can speed up the segmentation and present satisfactory results in cell image processing.

  1. Withdrawn library materials in the period 2005-2010 through the prism of the national library

    Directory of Open Access Journals (Sweden)

    Tereza Poličnik-Čermelj

    2011-01-01

    Full Text Available Purpose: In Slovenia, there is no national repository library for the long-term retention of withdrawn library materials. Therefore, the National and University Library (NUKcoordinates withdrawals in Slovenian libraries in order to preserve the national cultural heritage. The author highlights collaborative activities of NUK and other libraries to ensure preservation and retention of withdrawn printed materials in terms of professional legislation and guidelines. According to them NUK is authorised to select withdrawn items to be included in the "Slovenica" collection.Methodology/approach: The paper deals with withdrawal statistics of Slovenian libraries during the period 2005-2010. The data were collected by the Library System Development Centre at NUK in correlation with withdrawal lists sent to NUK by the libraries.Results: The results show that well-preserved but outdated materials are deselected by less than a half of public libraries, by 10 % of higher-education libraries, by a little bit more than 5 % of special libraries and only by 2 % of school libraries. The protocol for data checking, sending the materials to NUK, handling and selection criteria are explained. The advantages and disadvantages of including the withdrawn items into the NUK collection and the disposal of inappropriately selected materials are described.The results of acquiring the last copies of "Slovenica" from the withdrawn materials are more than satisfactory for NUK.Research limitation: As there are no similar models of acquisition among foreign national libraries, the study is limited to Slovenian library practice.Originality/practical implications: The paper also indicates the direction of future development.

  2. A novel mechanotactic 3D modeling of cell morphology

    International Nuclear Information System (INIS)

    Cell morphology plays a critical role in many biological processes, such as cell migration, tissue development, wound healing and tumor growth. Recent investigations demonstrate that, among other stimuli, cells adapt their shapes according to their substrate stiffness. Until now, the development of this process has not been clear. Therefore, in this work, a new three-dimensional (3D) computational model for cell morphology has been developed. This model is based on a previous cell migration model presented by the same authors. The new model considers that during cell–substrate interaction, cell shape is governed by internal cell deformation, which leads to an accurate prediction of the cell shape according to the mechanical characteristic of its surrounding micro-environment. To study this phenomenon, the model has been applied to different numerical cases. The obtained results, which are qualitatively consistent with well-known related experimental works, indicate that cell morphology not only depends on substrate stiffness but also on the substrate boundary conditions. A cell located within an unconstrained soft substrate (several kPa) with uniform stiffness is unable to adhere to its substrate or to send out pseudopodia. When the substrate stiffness increases to tens of kPa (intermediate and rigid substrates), the cell can adequately adhere to its substrate. Subsequently, as the traction forces exerted by the cell increase, the cell elongates and its shape changes. Within very stiff (hard) substrates, the cell cannot penetrate into its substrate or send out pseudopodia. On the other hand, a cell is found to be more elongated within substrates with a constrained surface. However, this elongation decreases when the cell approaches it. It can be concluded that the higher the net traction force, the greater the cell elongation, the larger the cell membrane area, and the less random the cell alignment. (paper)

  3. Library/vendor relationships

    CERN Document Server

    Brooks, Sam

    2014-01-01

    A view of the mutual dependence between libraries and vendorsAs technology advances, libraries are forced to reach beyond their own resources to find effective ways to maintain accuracy and superior service levels. Vendors provide databases and integrated library systems that perform those functions for profit. Library/Vendor Relationships examines the increasing cooperation in which libraries find they must participate in, and vice versa, with the vendors that provide system infrastructure and software. Expert contributors provide insights from all sides of this unique collaboration, offering

  4. Identification of two linear B-cell epitopes from West Nile virus NS1 by screening a phage-displayed random peptide library

    Directory of Open Access Journals (Sweden)

    Qin Yong-Li

    2011-07-01

    Full Text Available Abstract Background The West Nile virus (WNV nonstructural protein 1 (NS1 is an important antigenic protein that elicits protective antibody responses in animals and can be used for the serological diagnosis of WNV infection. Although previous work has demonstrated the vital role of WNV NS1-specific antibody responses, the specific epitopes in the NS1 have not been identified. Results The present study describes the identification of two linear B-cell epitopes in WNV NS1 through screening a phage-displayed random 12-mer peptide library with two monoclonal antibodies (mAbs 3C7 and 4D1 that directed against the NS1. The mAbs 3C7 and 4D1 recognized phages displaying peptides with the consensus motifs LTATTEK and VVDGPETKEC, respectively. Exact sequences of both motifs were found in the NS1 (895LTATTEK901 and 925VVDGPETKEC934. Further identification of the displayed B cell epitopes were conducted using a set of truncated peptides expressed as MBP fusion proteins. The data indicated that 896TATTEK901 and925VVDGPETKEC934 are minimal determinants of the linear B cell epitopes recognized by the mAbs 3C7 and 4D1, respectively. Antibodies present in the serum of WNV-positive horses recognized the minimal linear epitopes in Western blot analysis, indicating that the two peptides are antigenic in horses during infection. Furthermore, we found that the epitope recognized by 3C7 is conserved only among WNV strains, whereas the epitope recognized by 4D1 is a common motif shared among WNV and other members of Japanese encephalitis virus (JEV serocomplex. Conclusions We identified TATTEK and VVDGPETKEC as NS1-specific linear B-cell epitopes recognized by the mAbs 3C7 and 4D1, respectively. The knowledge and reagents generated in this study may have potential applications in differential diagnosis and the development of epitope-based marker vaccines against WNV and other viruses of JEV serocomplex.

  5. Design of Trojan Horse Detection Model Based on the Behavioral Library%基于行为特征库的木马检测模型设计

    Institute of Scientific and Technical Information of China (English)

    李焕洲; 陈婧婧; 钟明全; 唐彰国

    2011-01-01

    目前木马检测的主流技术主要是特征码检测技术,而该技术提取特征码滞后,无法检测未知新型木马.为了更好的检测新型木马,详细归纳总结木马的行为特征,同时在此基础上提取木马通适性行为特征,构建木马行为特征库,设计了基于行为特征库的木马检测模型,并应用模糊模式识别方法判断木马程序.通过实验证明此模型可以对可疑程序的行为特征进行分析判断,较准确地识别木马程序.该检测模型是对基于特征码检测技术的强有力补充,在新型木马不断涌现的今天,基于木马行为特征检测技术具有重要的应用意义.%At present, the mainstream technology of Trojan horse detection is the characteristic code detection technology, but this technology lags behind extracting characteristic code, it can not detect the new Trojans.In order to detect the new Trojans better, by summing up the Trojan behavioral characteristics in detail, and constructing the library of Trojan behavioral characteristics, the Trojan detection model which based on the behavioral library has been designed.It can judge Trojan making use of fuzzy pattern recognition.Experiments prove that this model can analyze and judge the behavior of suspicious program, identify the Trojan.This detection technolgy has great value in practical application.

  6. Couplerlib: a metadata-driven library for the integration of multiple models of higher and lower trophic level marine systems with inexact functional group matching

    Science.gov (United States)

    Beecham, Jonathan; Bruggeman, Jorn; Aldridge, John; Mackinson, Steven

    2016-03-01

    End-to-end modelling is a rapidly developing strategy for modelling in marine systems science and management. However, problems remain in the area of data matching and sub-model compatibility. A mechanism and novel interfacing system (Couplerlib) is presented whereby a physical-biogeochemical model (General Ocean Turbulence Model-European Regional Seas Ecosystem Model, GOTM-ERSEM) that predicts dynamics of the lower trophic level (LTL) organisms in marine ecosystems is coupled to a dynamic ecosystem model (Ecosim), which predicts food-web interactions among higher trophic level (HTL) organisms. Coupling is achieved by means of a bespoke interface, which handles the system incompatibilities between the models and a more generic Couplerlib library, which uses metadata descriptions in extensible mark-up language (XML) to marshal data between groups, paying attention to functional group mappings and compatibility of units between models. In addition, within Couplerlib, models can be coupled across networks by means of socket mechanisms. As a demonstration of this approach, a food-web model (Ecopath with Ecosim, EwE) and a physical-biogeochemical model (GOTM-ERSEM) representing the North Sea ecosystem were joined with Couplerlib. The output from GOTM-ERSEM varies between years, depending on oceanographic and meteorological conditions. Although inter-annual variability was clearly present, there was always the tendency for an annual cycle consisting of a peak of diatoms in spring, followed by (less nutritious) flagellates and dinoflagellates through the summer, resulting in an early summer peak in the mesozooplankton biomass. Pelagic productivity, predicted by the LTL model, was highly seasonal with little winter food for the higher trophic levels. The Ecosim model was originally based on the assumption of constant annual inputs of energy and, consequently, when coupled, pelagic species suffered population losses over the winter months. By contrast, benthic populations

  7. Accelerated discovery via a whole-cell model.

    Science.gov (United States)

    Sanghvi, Jayodita C; Regot, Sergi; Carrasco, Silvia; Karr, Jonathan R; Gutschow, Miriam V; Bolival, Benjamin; Covert, Markus W

    2013-12-01

    To test the promise of whole-cell modeling to facilitate scientific inquiry, we compared growth rates simulated in a whole-cell model with experimental measurements for all viable single-gene disruption Mycoplasma genitalium strains. Discrepancies between simulations and experiments led to predictions about kinetic parameters of specific enzymes that we subsequently validated. These findings represent, to our knowledge, the first application of whole-cell modeling to accelerate biological discovery. PMID:24185838

  8. Human pluripotent stem cells: an emerging model in developmental biology

    OpenAIRE

    Zhu, Zengrong; Huangfu, Danwei

    2013-01-01

    Developmental biology has long benefited from studies of classic model organisms. Recently, human pluripotent stem cells (hPSCs), including human embryonic stem cells and human induced pluripotent stem cells, have emerged as a new model system that offers unique advantages for developmental studies. Here, we discuss how studies of hPSCs can complement classic approaches using model organisms, and how hPSCs can be used to recapitulate aspects of human embryonic development ‘in a dish’. We also...

  9. A numerical semiconductor model applicable to organic solar cells

    OpenAIRE

    Minnaert, Ben; Burgelman, Marc; Heereman, Frédéric

    2007-01-01

    Excitons are marginally important in classical semiconductor device physics, and their treatment is not included in standard solar cell modelling. However, in organic semiconductors and solar cells, the role of excitons is essential, as the primary effect of light absorption is exciton generation, and free electrons and holes are created by exciton dissociation. First steps to include excitons in solar cell modelling were presented by Green and Zhang. We extended their model (2006), includin...

  10. Designing Syntax Embeddings and Assimilations for Language Libraries

    NARCIS (Netherlands)

    Bravenboer, M.; Visser, E.

    2009-01-01

    Preprint of paper published in: Models in Software Engineering, Lecture Notes in Computer Science 5002, 2008; doi:10.1007/978-3-540-69073-3_5 Language libraries extend regular libraries with domain-specific notation. More precisely, a language library is a combination of a domain-specific language

  11. Content and Workflow Management for Library Websites: Case Studies

    Science.gov (United States)

    Yu, Holly, Ed.

    2005-01-01

    Using database-driven web pages or web content management (WCM) systems to manage increasingly diverse web content and to streamline workflows is a commonly practiced solution recognized in libraries today. However, limited library web content management models and funding constraints prevent many libraries from purchasing commercially available…

  12. Five Aspects of Current Trends in German Library Science

    Science.gov (United States)

    Steierwald, Ulrike

    2006-01-01

    The specialisation Library Science at the Hochschule Darmstadt/University of Applied Science Darmstadt is the newest academic program in Germany for the higher education of librarians. Five current trends in library science in Germany reflect the new "Darmstadt Model": (1) The delimitation of a specific professional field "library" is obsolete, so…

  13. What Is Your Budget Saying about Your Library?

    Science.gov (United States)

    Jacobs, Leslie; Strouse, Roger

    2002-01-01

    Discusses budgeting for corporate libraries and how to keep budgets from getting cut. Topics include whether the budget is considered corporate overhead; recovering costs; models for content cost recovery; showing return on library investment; marketing library value to senior management; user needs and satisfaction; and comparing budgets to other…

  14. The participatory public library

    DEFF Research Database (Denmark)

    Rasmussen, Casper Hvenegaard

    2016-01-01

    Purpose From collection to connection has been a buzzword in the library world for more than a decade. This catchy phrase indicates that users are seen not only as borrowers, but as active participants. The aim of this paper is to investigate and analyse three questions in relation to user...... participation in public libraries in a Nordic perspective. How can participation in public libraries be characterised? Why should libraries deal with user participation? What kinds of different user participation can be identified in public libraries? Design/methodology/approach The paper uses a selection...... of theoretical approaches and practical examples to obtain a varied understanding of user participation in public libraries. Research fields outside library and information science have developed a wide range of theoretical approaches on user participation. Examples from cultural policy, museum studies...

  15. Libraries in Society

    DEFF Research Database (Denmark)

    Kristiansson, Michael; Skouvig, Laura

    brought about by the boom of the internet and the advent of the post-modern globalised knowledge-based and network-organised society. Finally, the paper outlines how theoretical and strategic library development can benefit from academic considerations on the dialectics between openness and restrictedness......The purpose of the paper is to investigate the phenomenon of openness in relation to library development. The term openness is presented and related to library development from historical and theoretical perspectives. The paper elaborates on the differences over time on to how openness has been...... understood in a library setting. Historically, openness in form of the open shelves played a crucial role in developing the modern public library. The paper examines this openness-centred library policy as adopted by Danish public libraries in the beginning of the 20th century by applying the theories...

  16. E-book Reader Devices and Libraries

    Directory of Open Access Journals (Sweden)

    Pažur, I.

    2011-03-01

    Full Text Available Most library studies thematically related to electronic books don't consider readers of electronic books. Only in recent years librarians conduct studies in which they want to find out readers' opinions about the possibilities, advantages and disadvantages of reading using e-readers, as well as their possible application in the libraries.User studies of e-readers have shown that their opinion is generally positive, but great attachment to traditional books is still present, e-readers are still seen only as an additional tool for reading. Sony with its e-reader (the latest Reader model Daily and Reader Store online bookstore (http://ebookstore.sony.com/is the only one who cooperate with libraries and has made lending electronic books possible. Cooperation was launched in 2009th,and the New York Public Library was the first library that offered such a service.Cooperation between Sony and libraries, indicates clearly what the near future could be if other online booksellers / publishers begin to follow the model of lending e-books through the libraries over the network. However it is possible that a large online bookstores / publishers consider that the further price reduction of e-readers and electronic books will constantly increase their sales, and in that case lending e-books will be unnecessary.Are the libraries ready for this scenario?

  17. One library to make them all: Streamlining yeast library creation by a SWAp-Tag (SWAT) strategy

    Science.gov (United States)

    Zalckvar, Einat; Goldman, Omer; Ben-Dor, Shifra; Schütze, Conny; Wiedemann, Nils; Knop, Michael; Khmelinskii, Anton; Schuldiner, Maya

    2016-01-01

    The yeast Saccharomyces cerevisiae is ideal for systematic studies relying on collections of modified strains (libraries). Despite the significance of yeast libraries and the immense variety of available tags and regulatory elements, only a few such libraries exist as their construction is extremely expensive and laborious. To overcome these limitations we developed a SWAp-Tag method (SWAT), in which one parental library can be modified easily and efficiently to give rise to an endless variety of libraries of choice. We showcase the versatility of the SWAT approach by constructing and investigating a library of ~1,800 strains carrying a SWAT-GFP module at the amino termini of endomembrane proteins and then using it to create two new libraries (mCherry or seamless GFP). Our work demonstrates how the SWAT method enables fast and effortless creation of yeast libraries, opening the door for endless new ways to systematically study cell biology. PMID:26928762

  18. One library to make them all: streamlining the creation of yeast libraries via a SWAp-Tag strategy.

    Science.gov (United States)

    Yofe, Ido; Weill, Uri; Meurer, Matthias; Chuartzman, Silvia; Zalckvar, Einat; Goldman, Omer; Ben-Dor, Shifra; Schütze, Conny; Wiedemann, Nils; Knop, Michael; Khmelinskii, Anton; Schuldiner, Maya

    2016-04-01

    The yeast Saccharomyces cerevisiae is ideal for systematic studies relying on collections of modified strains (libraries). Despite the significance of yeast libraries and the immense variety of available tags and regulatory elements, only a few such libraries exist, as their construction is extremely expensive and laborious. To overcome these limitations, we developed a SWAp-Tag (SWAT) method that enables one parental library to be modified easily and efficiently to give rise to an endless variety of libraries of choice. To showcase the versatility of the SWAT approach, we constructed and investigated a library of ∼1,800 strains carrying SWAT-GFP modules at the amino termini of endomembrane proteins and then used it to create two new libraries (mCherry and seamless GFP). Our work demonstrates how the SWAT method allows fast and effortless creation of yeast libraries, opening the door to new ways of systematically studying cell biology.

  19. Flight Software Math Library

    Science.gov (United States)

    McComas, David

    2013-01-01

    The flight software (FSW) math library is a collection of reusable math components that provides typical math utilities required by spacecraft flight software. These utilities are intended to increase flight software quality reusability and maintainability by providing a set of consistent, well-documented, and tested math utilities. This library only has dependencies on ANSI C, so it is easily ported. Prior to this library, each mission typically created its own math utilities using ideas/code from previous missions. Part of the reason for this is that math libraries can be written with different strategies in areas like error handling, parameters orders, naming conventions, etc. Changing the utilities for each mission introduces risks and costs. The obvious risks and costs are that the utilities must be coded and revalidated. The hidden risks and costs arise in miscommunication between engineers. These utilities must be understood by both the flight software engineers and other subsystem engineers (primarily guidance navigation and control). The FSW math library is part of a larger goal to produce a library of reusable Guidance Navigation and Control (GN&C) FSW components. A GN&C FSW library cannot be created unless a standardized math basis is created. This library solves the standardization problem by defining a common feature set and establishing policies for the library s design. This allows the libraries to be maintained with the same strategy used in its initial development, which supports a library of reusable GN&C FSW components. The FSW math library is written for an embedded software environment in C. This places restrictions on the language features that can be used by the library. Another advantage of the FSW math library is that it can be used in the FSW as well as other environments like the GN&C analyst s simulators. This helps communication between the teams because they can use the same utilities with the same feature set and syntax.

  20. An HSV-based library screen identifies PP1α as a negative TRPV1 regulator with analgesic activity in models of pain

    Science.gov (United States)

    Reinhart, Bonnie; Goins, William F; Harel, Asaff; Chaudhry, Suchita; Goss, James R; Yoshimura, Naoki; de Groat, William C; Cohen, Justus B; Glorioso, Joseph C

    2016-01-01

    Transient receptor potential vanilloid 1 (TRPV1) is a pronociceptive cation channel involved in persistent inflammatory and neuropathic pain. Herpes simplex virus (HSV) vector expression of TRPV1 causes cell death in the presence of capsaicin, thereby completely blocking virus replication. Here we describe a selection system for negative regulators of TRPV1 based on rescue of virus replication. HSV-based coexpression of TRPV1 and a PC12 cell-derived cDNA library identified protein phosphatase 1α (PP1α) as a negative regulator of TRPV1, mimicking the activity of “poreless” (PL), a dominant-negative mutant of TRPV1. Vectors expressing PP1α or PL reduced thermal sensitivity following virus injection into rat footpads, but failed to reduce the nocifensive responses to menthol/icilin-activated cold pain or formalin, demonstrating that the activity identified in vitro is functional in vivo with a degree of specificity. This system should prove powerful for identifying other cellular factors that can inhibit ion channel activity. PMID:27382601

  1. Modelling Neurodegenerative Diseases Using Human Pluripotent Stem Cells

    DEFF Research Database (Denmark)

    Hall, Vanessa J.

    2016-01-01

    Neurodegenerative diseases are being modelled in-vitro using human patient-specific, induced pluripotent stem cells and transgenic embryonic stem cells to determine more about disease mechanisms, as well as to discover new treatments for patients. Current research in modelling Alzheimer’s disease......, frontotemporal dementia and Parkinson’s disease using pluripotent stem cells is described, along with the advent of gene-editing, which has been the complimentary tool for the field. Current methods used to model these diseases are predominantly dependent on 2D cell culture methods. Outcomes reveal that only...... that includes studying more complex 3D cell cultures, as well as accelerating aging of the neurons, may help to yield stronger phenotypes in the cultured cells. Thus, the use and application of pluripotent stem cells for modelling disease have already shown to be a powerful approach for discovering more about...

  2. Designing Syntax Embeddings and Assimilations for Language Libraries

    OpenAIRE

    Bravenboer, M.; Visser, E.

    2009-01-01

    Preprint of paper published in: Models in Software Engineering, Lecture Notes in Computer Science 5002, 2008; doi:10.1007/978-3-540-69073-3_5 Language libraries extend regular libraries with domain-specific notation. More precisely, a language library is a combination of a domain-specific language embedded in the general-purpose host language, a regular library implementing the underlying functionality, and an assimilation transformation that maps embedded DSL fragments to host language code....

  3. Modeling the Chagas’ disease after stem cell transplantation

    Science.gov (United States)

    Galvão, Viviane; Miranda, José Garcia Vivas

    2009-04-01

    A recent model for Chagas’ disease after stem cell transplantation is extended for a three-dimensional multi-agent-based model. The computational model includes six different types of autonomous agents: inflammatory cell, fibrosis, cardiomyocyte, proinflammatory cytokine tumor necrosis factor- α, Trypanosoma cruzi, and bone marrow stem cell. Only fibrosis is fixed and the other types of agents can move randomly through the empty spaces using the three-dimensional Moore neighborhood. Bone marrow stem cells can promote apoptosis in inflammatory cells, fibrosis regression and can differentiate in cardiomyocyte. T. cruzi can increase the number of inflammatory cells. Inflammatory cells and tumor necrosis factor- α can increase the quantity of fibrosis. Our results were compared with experimental data giving a fairly fit and they suggest that the inflammatory cells are important for the development of fibrosis.

  4. Mathematical models in cell biology and cancer chemotherapy

    CERN Document Server

    Eisen, Martin

    1979-01-01

    The purpose of this book is to show how mathematics can be applied to improve cancer chemotherapy. Unfortunately, most drugs used in treating cancer kill both normal and abnormal cells. However, more cancer cells than normal cells can be destroyed by the drug because tumor cells usually exhibit different growth kinetics than normal cells. To capitalize on this last fact, cell kinetics must be studied by formulating mathematical models of normal and abnormal cell growth. These models allow the therapeutic and harmful effects of cancer drugs to be simulated quantitatively. The combined cell and drug models can be used to study the effects of different methods of administering drugs. The least harmful method of drug administration, according to a given criterion, can be found by applying optimal control theory. The prerequisites for reading this book are an elementary knowledge of ordinary differential equations, probability, statistics, and linear algebra. In order to make this book self-contained, a chapter on...

  5. Assessing Library Automation and Virtual Library Development in Four Academic Libraries in Oyo, Oyo State, Nigeria

    Science.gov (United States)

    Gbadamosi, Belau Olatunde

    2011-01-01

    The paper examines the level of library automation and virtual library development in four academic libraries. A validated questionnaire was used to capture the responses from academic librarians of the libraries under study. The paper discovers that none of the four academic libraries is fully automated. The libraries make use of librarians with…

  6. Development of static constitutive model library for soils based on ABAQUS%基于ABAQUS建立土体本构模型库的研究

    Institute of Scientific and Technical Information of China (English)

    司海宝; 蔡正银

    2011-01-01

    ABAQUS作为一种强大的通用有限元分析程序,具有很强的非线性计算功能和前、后处理能力.但标准ABAQUS程序中的土体本构模型,没能反映土体的剪胀、软化与硬化、应力路径对变形的影响等,因此,有必要在ABAQUS中开发出更适合于土体特性的本构模型库.为拓展ABAQUS在岩土工程领域的计算能力,利用二次开发工具UMAT数据接口,开发出更适合于工程应用的土体本构模型库,如邓肯模型、南水模型及状态相关的砂土模型,不仅可以充分利用ABAQUS程序方便、快捷的前后处理和强大的非线性求解平台,而且还可以完成更有针对性、更切合实际土体应力一应变有限元的数值计算,为工程实践提供技术支持.从计算过程、模拟结果与试验结果比较来看,二次开发模型库子程序运行稳定,模拟结果正确、可靠.%As a powerful universal finite element analysis program, ABAQUS possesses immense ability for nonlinear computing, pre-and-post-processing.But the constitutive model of soil adopted in standard ABAQUS is inadequate in terms of the inability of reflecting militancy of cohesive soil, strain-softening and hardening of sand as well as the impact of stress path on deformation, etc.So it became an emergency for new constitutive model library which is more suitable for soil to be added into ABAQUS.In order to enlarge the computing ability of ABAQUS in the area of geotechnical engineering, new constitutive model library such as Duncan-Chang model, NHRI model and Sand model which are more adaptive for engineering application were developed with the help of secondary development toolkit UMAT as well as data interface.In this way, the convenient pre-and-post-processing and powerful nonlinear solving platform were made full use of in the study, and that more pertinent and more realistic finite element calculation for the stress and strain in soil can be accomplished, which is able to provide

  7. Induced pluripotent stem cells and Parkinson's disease: modelling and treatment.

    Science.gov (United States)

    Xu, Xiaoyun; Huang, Jinsha; Li, Jie; Liu, Ling; Han, Chao; Shen, Yan; Zhang, Guoxin; Jiang, Haiyang; Lin, Zhicheng; Xiong, Nian; Wang, Tao

    2016-02-01

    Many neurodegenerative disorders, such as Parkinson's disease (PD), are characterized by progressive neuronal loss in different regions of the central nervous system, contributing to brain dysfunction in the relevant patients. Stem cell therapy holds great promise for PD patients, including with foetal ventral mesencephalic cells, human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). Moreover, stem cells can be used to model neurodegenerative diseases in order to screen potential medication and explore their mechanisms of disease. However, related ethical issues, immunological rejection and lack of canonical grafting protocols limit common clinical use of stem cells. iPSCs, derived from reprogrammed somatic cells, provide new hope for cell replacement therapy. In this review, recent development in stem cell treatment for PD, using hiPSCs, as well as the potential value of hiPSCs in modelling for PD, have been summarized for application of iPSCs technology to clinical translation for PD treatment.

  8. Computational modeling of the spatiotemporal dynamics of cancer stem cells

    Science.gov (United States)

    Signoriello, Alexandra; Bosenberg, Marcus; Shattuck, Mark; O'Hern, Corey

    2015-03-01

    Cancer stem cells can differentiate into any cell type in a particular tumor, and thus can reform a tumor even when seeded from a single cell. Despite their importance, the identification of stem cells, their interactions, and how and why they malfunction to cause cancer and form tumors are not well understood. We have developed discrete element modeling (DEM) simulations to investigate the role of stem cells in the formation of heterogeneous cell populations in melanoma tumors. The DEM simulations include elastic, excluded volume, and signaling interactions between cells and rates for cell differentiation, apoptosis, and growth. The DEM is calibrated to results from experimental studies of melanoma tumor growth in mouse models. We use the simulations to generate virtual tumors and study their morphology and cell subtype populations as a function of time.

  9. GIBS Geospatial Data Abstraction Library (GDAL)

    Data.gov (United States)

    National Aeronautics and Space Administration — GDAL is an open source translator library for raster geospatial data formats that presents a single abstract data model to the calling application for all supported...

  10. A C++ library for Multimodal Deep Learning

    OpenAIRE

    Jin, Jian

    2015-01-01

    MDL, Multimodal Deep Learning Library, is a deep learning framework that supports multiple models, and this document explains its philosophy and functionality. MDL runs on Linux, Mac, and Unix platforms. It depends on OpenCV.

  11. LIBRARY SURVEY 2001

    CERN Multimedia

    2001-01-01

    The primary role of the library is to make sure that you can do YOUR work in the most efficient way possible. To ensure that we continue to match our services to your information needs, the library regularly gathers the views and opinions of its readers in a variety of ways, [link for e-version: http://library/library_general/statistics/library_statistics_ surveys.html], including user surveys. The last survey was carried out in 1996. One of the most visible results of that survey was the extension of the library desk service until seven o'clock in the evening, to meet the demand for greater access to library materials. Now the 'electronic library' is becoming more important than the physical one, we feel it is once again time to ensure that we are providing the services and information you need, in the most effective way possible. We also want to make sure you are aware of the full range of services that the library provides. Please spare just a few minutes to fill out our survey at http://library.cern.ch/su...

  12. News from the Library

    CERN Multimedia

    CERN Library

    2010-01-01

    The LHC Library to be merged with the Central Library. Not everyone knows that CERN Scientific Information Service currently counts three physical libraries on site. The Central Library is located in Building 52 and there are two satellite libraries located respectively in building 30 (the LHC Library) and in building 864 on Prévessin site (the SPS Library). Moreover, the Legal Service Library is located in Building 60. In the past, there have been at CERN up to 6 satellite libraries; they were essential at a time when information was only in paper form and having multiple copies of documents located in several places at CERN was useful to facilitate scientific research. Today, this need is less critical as most of our resources are online. That is why, following a SIPB (Scientific Information Policy Board) decision, the collections of the LHC Library will be merged this summer with the Central collection. This reorganization and centralization of resources will improve loan services. The SP...

  13. Modeling the mechanics of cells in the cell-spreading process driven by traction forces.

    Science.gov (United States)

    Fang, Yuqiang; Lai, King W C

    2016-04-01

    Mechanical properties of cells and their mechanical interaction with the extracellular environments are main factors influencing cellular function, thus indicating the progression of cells in different disease states. By considering the mechanical interactions between cell adhesion molecules and the extracellular environment, we developed a cell mechanical model that can characterize the mechanical changes in cells during cell spreading. A cell model was established that consisted of various main subcellular components, including cortical cytoskeleton, nuclear envelope, actin filaments, intermediate filaments, and microtubules. We demonstrated the structural changes in subcellular components and the changes in spreading areas during cell spreading driven by traction forces. The simulation of nanoindentation tests was conducted by integrating the indenting force to the cell model. The force-indentation curve of the cells at different spreading states was simulated, and the results showed that cell stiffness increased with increasing traction forces, which were consistent with the experimental results. The proposed cell mechanical model provides a strategy to investigate the mechanical interactions of cells with the extracellular environments through the adhesion molecules and to reveal the cell mechanical properties at the subcellular level as cells shift from the suspended state to the adherent state. PMID:27176326

  14. E-Services in Danish Research Libraries

    DEFF Research Database (Denmark)

    Scupola, Ada

    2008-01-01

    This chapter reports the findings of a case study of e-services adoption at research libraries. The case under consideration is Roskilde University Library (RUB), a research library supporting learning activities at Roskilde University. The research focuses on the main issues that RUB had to deal...... with in the process of adopting e-services and the future challenges that e-services provide for RUB. The chapter also presents the consequences of e-services adoption for Roskilde University library's organization, its business model and the relationships with customers, publishers (providers of knowledge) and other...... research libraries in Denmark. The main results can be summarized as follows: 1) Adoption of e-services has forced RUB to innovate rapidly. Innovation is driven, among other factors, by ICT developments (technology push), but innovation is also user-driven and pervasive throughout the organization.  2) E-services...

  15. The Influence and Inspiration of "Qingfanqie" Model for the Public Library%“青番茄”模式对公共图书馆的影响与启示

    Institute of Scientific and Technical Information of China (English)

    吴畅

    2012-01-01

    The development of " Qingfanqie" internet library brings a brand - new concept impact and realistic influence on the traditional public library, the thesis analyzes the advantages and disad- vantages of " Qingfanqie" internet library and public library by comparison. It argues that public li- braries have to innovate ideas and services to perfect the general and subsidiary library institution system and to promote the service model of network distribution.%“青番茄”网络图书馆的发展给传统的公共图书馆带来了全新的观念冲击与现实的影响,本文对青番茄网络图书馆与公共图书馆各自的优劣势进行了比较分析,认为应对网络图书馆的冲击,公共图书馆必须更新理念、创新服务,完善图书资源共享的总分馆制度以及推广网络化的配送服务模式。

  16. E-learning resource library model based on domain ontology%基于领域本体的电子学习资源库模型

    Institute of Scientific and Technical Information of China (English)

    张沪寅; 张铭洋; 李鑫

    2012-01-01

    随着电子学习系统快速的发展,电子学习资源呈现爆炸式的增长,如何有效地组织海量电子学习资源成为构建高效电子学习系统的重要因素.针对现有资源库在资源组织方面存在的不足,提出了一个基于领域知识本体的电子学习资源库检索模型,该模型利用领域知识来构建领域知识本体库并通过抽取电子学习资源元数据构建元数据库,通过映射关系完成对电子学习资源的语义组织,并在此基础之上构建一个语义检索模型,以有效地解决现有电子学习资源检索中丢失语义背景的问题,使检索结果在查全率、查准率方面有所提高,更加符合用户的需求.%With the rapid development of E-learning system, E-learning resources grow explosively. How to effectively organize E-learning resources is a key factor of constructing efficient E-learning system. Concerning the existing resources organization deficiency of E-learning resource library, this paper proposed an E-learning resource retrieval model based on domain ontology. This model built a domain knowledge library by making use of the domain knowledge and constructed E-learning resources metadata database by extracting resources metadata, realized semantic organization of E-learning resources through mapping relations, and constructed a semantic retrieval model on this basis, in order to effectively solve the problem of the loss of semantic background in the E-learning resource retrieving. The model has also enhanced the recall rate and the precision rate on the retrieval results, and it is more in line with the needs of the users.

  17. Combined Monte Carlo and path-integral method for simulated library of time-resolved reflectance curves from layered tissue models

    Science.gov (United States)

    Wilson, Robert H.; Vishwanath, Karthik; Mycek, Mary-Ann

    2009-02-01

    Monte Carlo (MC) simulations are considered the "gold standard" for mathematical description of photon transport in tissue, but they can require large computation times. Therefore, it is important to develop simple and efficient methods for accelerating MC simulations, especially when a large "library" of related simulations is needed. A semi-analytical method involving MC simulations and a path-integral (PI) based scaling technique generated time-resolved reflectance curves from layered tissue models. First, a zero-absorption MC simulation was run for a tissue model with fixed scattering properties in each layer. Then, a closed-form expression for the average classical path of a photon in tissue was used to determine the percentage of time that the photon spent in each layer, to create a weighted Beer-Lambert factor to scale the time-resolved reflectance of the simulated zero-absorption tissue model. This method is a unique alternative to other scaling techniques in that it does not require the path length or number of collisions of each photon to be stored during the initial simulation. Effects of various layer thicknesses and absorption and scattering coefficients on the accuracy of the method will be discussed.

  18. A complete library of X-ray pulsars in the Magellanic Clouds: A new resource for modeling the time evolution of luminosity and pulse profile

    Science.gov (United States)

    Yang, Jun; Laycock, Silas; Christodoulou, Dimitris; Fingerman, Samuel; Cappallo, Rigel; Zezas, Andreas; Antoniou, Vallia; Hong, Jaesub; Ho, Wynn; Coe, Malcolm; Klus, Helen

    2016-01-01

    We have collected and analyzed all XMM-Newton and Chandra (˜ 300) observations of the known pulsars in the Small & Large Magellanic Clouds (SMC, LMC). We aim to classify various pulsar properties with amplitude logLX = 33 ˜ 38 erg/s and incorporate the related parameters in theoretical models. With the high time-resolution data from the European Photon Imaging Camera (EPIC) and the latest calibration files and the Science Analysis System (SAS) software from High Energy Astrophysics Science Archive Research Center Software (HEASOFT), our pipeline generates a suite of useful products for each pulsar detection: point-source event lists, pulse profiles, periodograms, and spectra for the broad energy band, the soft band (0.2-2 keV), and the hard band (2-12 keV). Of 59 SMC pulsars in the EPIC field of view, we were able to measure 29 with pulse periods and power spectra. From XMM for example, for 16 of them, we find 12 are spinning up and 4 are spinning down. We also compare the observed pulse profiles to geometric models of the pulsars in order to constrain the magnetospheric parameters of each of these sources. Our motivation is to provide a library for time domain studies and profile modeling.

  19. A Study on Library Information Service Model Based on Internet+ Contextualized User Proifle%互联网+图书馆情景化用户偏好的信息服务模式研究

    Institute of Scientific and Technical Information of China (English)

    黄传慧

    2016-01-01

    Internet is profoundly changing the way everyone works and lives. According to the research status at home and abroad this paper analyzes Internet + technology basis, describes the strategy of the information service in library under the of Internet + environment that structures library information service model based on contextualized user proifle in order to promote the development of library information service .%互联网+正在深刻地改变着每一个人的工作和生活方式。文章剖析了互联网+的技术基础,并提出互联网+环境下图书馆信息服务策略,最后构建了互联网+环境下图书馆情景化用户偏好信息服务模式,以期促进图书馆信息服务的发展。

  20. The Library International Partnerweek 2011

    DEFF Research Database (Denmark)

    Presentation at the Library International Partnerweek, held at Copenhagen Technical Library at the Copenhagen University College of Engineering. Participant: Ms. Carmen Priesto Estravid from Madrid Technical University, E.U.I.T. Obras Públicas, Library. Spain Ms.Tuulikki Hattunen from TUAS Library....... Finland Ms. Anitta Ôrm from Kemi-Tornio UAS Library. Finland Mr. Manfred Walter from HTW-Berlin. Germany Mr. Peter Hald from Copenhagen Technical Library. Denmark Mr. Ole Micahelsen from Copenhagen Technical Library. Denmark...