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Sample records for cell mechanoelectrical transducer

  1. Mechanoelectrical Transducer Has Discrete Conductances in the Chick Vestibular Hair Cell

    Science.gov (United States)

    Ohmori, Harunori

    1984-03-01

    Properties of mechanoelectrical transduction were studied at the single-cell level by applying a whole-cell recording variation of the patch-clamp technique to dissociated vestibular hair cells of chicks. The hair bundle was directly stimulated by a glass rod, and transduction currents were recorded from the cell body. After a triangular movement of the stimulating probe, the transduction current was generated stepwise between discrete levels of amplitude. The minimum step amplitude was -1.8 pA at -27 mV in Na-containing normal saline.

  2. Mechano-electric optoisolator transducer with hysteresis

    Science.gov (United States)

    Ciuruş, I. M.; Dimian, M.; Graur, A.

    2011-01-01

    This article presents a theoretical and experimental study of designing a mechano-electric optoisolator transducer with hysteresis. Our research is centred upon designing transducers on the basis of optical sensors, as photoelectric conversions eliminate the influence of electromagnetic disturbances. Conversion of the rotation/translation motions into electric signals is performed with the help of a LED-photoresistor Polaroid optocoupler. The driver of the optocoupler's transmitter module is an independent current source. The signal conditioning circuit is a Schmitt trigger circuit. The device is designed to be applied in the field of automation and mechatronics.

  3. A descriptive model of the receptor potential nonlinearities generated by the hair cell mechanoelectrical transducer.

    Science.gov (United States)

    Lukashkin, A N; Russell, I J

    1998-02-01

    This paper describes a model for generating the hair cell receptor potential based on a second-order Boltzmann function. The model includes only the resistive elements of the hair cell membranes with batteries across them and the series resistance of the external return path of the transducer current through the tissue of the cochlea. The model provides a qualitative description of signal processing by the hair cell transducer and shows that the nonlinearity of the hair cell transducer can give rise to nonlinear phenomena, such as intermodulation distortion products and two-tone suppression with patterns similar to those which have been recorded from the peripheral auditory system. Particular outcomes of the model are the demonstration that two-tone suppression depends not on the saturation of the receptor current, but on the behaviour of the hair cell transducer function close to the operating point. The model also shows that there is non-monotonic growth and phase change for any spectral component, but not for the fundamental of the receptor potential. PMID:9479750

  4. Harmonin-b, an actin-binding scaffold protein, is involved in the adaptation of mechanoelectrical transduction by sensory hair cells

    OpenAIRE

    Michalski, Nicolas; Michel, Vincent; Caberlotto, Elisa; Lefèvre, Gaelle M.; Van Aken, Alexander F J; Tinevez, Jean-Yves; Bizard, Emilie; Houbron, Christophe; Weil, Dominique; Hardelin, Jean-Pierre; Richardson, Guy P.; Kros, Corné J.; Martin, Pascal; Petit, Christine

    2009-01-01

    We assessed the involvement of harmonin-b, a submembranous protein containing PDZ domains, in the mechanoelectrical transduction machinery of inner ear hair cells. Harmonin-b is located in the region of the upper insertion point of the tip link that joins adjacent stereocilia from different rows and that is believed to gate transducer channel(s) located in the region of the tip link's lower insertion point. In Ush1c dfcr-2J/dfcr-2J mutant mice defective for harmonin-b, step deflections of the...

  5. Mechanoelectric effect in solid electrolytes

    International Nuclear Information System (INIS)

    The mechanoelectric effect in solid electrolytes of the compositions ZrO2 + 8 mol % Sc2O3 and ZrO2 + 8 mol % Y2O3 is investigated experimentally. The mechanical properties of polycrystalline specimens are studied using four-point bending in air. It is shown that a negative charge is induced on the extended side of the bent specimen and that the magnitude of this charge depends on the external mechanical load and the temperature of measurement. The assumption is made that the observed phenomena are associated with the uphill diffusion of vacancies in response to a nonuniform field of mechanical stresses. The theoretical model is compared with the experimental results

  6. Pax4 Expression does not Transduce Pancreatic Alpha Cells to Beta Cells

    Directory of Open Access Journals (Sweden)

    Ling Chen

    2015-07-01

    Full Text Available Background/Aims: The lack of available beta cells greatly limits the use of beta cell transplantation as a therapy for diabetes. Thus, generation of beta cells from other sources is substantially required. Pax4 has been shown to induce reprograming of alpha cells into beta cells during embryogenesis. Nevertheless, whether expression of Pax4 in adult alpha cells could trigger this alpha-to-beta cell reprogramming is unknown. Methods: Here we generated an adeno-associated virus carrying Pax4 and GFP under a CMV promoter (AAV-Pax4. We used AAV-Pax4 to transduce a mouse alpha cell line in vitro, and to transduce primary alpha cells in diabetic mice. Reprogramming was examined by double immunostaining and by changes in beta cell number. The effects on blood glucose were evaluated by fasting blood glucose and glucose response. Results: In vitro, Pax4 overexpression neither induced insulin expression, nor suppressed glucagon expression in alpha cells. In vivo, Pax4 overexpression failed to increase beta cell number, and did not alter hyperglycemia and glucose response in diabetic mice. Conclusion: Pax4 expression is not sufficient to transduce pancreatic alpha cells into beta cells. Overexpression of Pax4 in alpha cells may not increase functional beta cell number in diabetic patients.

  7. Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System

    OpenAIRE

    Li, Mingjie; Husic, Nada; Lin, Ying; Snider, B. Joy

    2012-01-01

    Efficient gene delivery in the central nervous system (CNS) is important in studying gene functions, modeling neurological diseases and developing therapeutic approaches. Lentiviral vectors are attractive tools in transduction of neurons and other cell types in CNS as they transduce both dividing and non-dividing cells, support sustained expression of transgenes, and have relatively large packaging capacity and low toxicity 1-3. Lentiviral vectors have been successfully used in transducing ma...

  8. EXPRESSION OF rhBMP-7 GENE IN TRANSDUCED BONE MARROW DERIVED STROMAL CELLS

    Institute of Scientific and Technical Information of China (English)

    段德宇; 杜靖远; 王洪; 刘勇; 郭晓东

    2002-01-01

    Objective. To explore the possibility of expression of exogenous gene in transduced bone marrow derived stromal cells(BMSCs). Methods. The marker gene , pbLacZ, was transferred into cultured BMSCs and the expression of transduced gene by X-gal staining was examined. Then plasmid pcDNA3-rhBMP7 was delivered to cultured BMSCs. Through immunohistochemical staining and RT-PCR assay, the expression of rhBMP7 gene was detected. Results. The exogenous gene could be expressed efficiently in transduced BMSCs. Conculsion. The present study provided a theoretical basis to gene therapy on the problems of bone and cartilage tissue.

  9. Generation of cytotoxic T cell against HBcAg using retrovirally transduced dendritic cells

    Institute of Scientific and Technical Information of China (English)

    Chuan-Lin Ding; Kun Yao; Tian-Tai Zhang; Feng Zhou; Lin Xu; Jiang-Ying Xu

    2003-01-01

    AIM: Cytotoxic T lymphocytes (CTLs) play an important role in resolving HBV infection. In the present study, we attempted to evaluate the efficiency of bone marrow-derived dendritic cells (DCs) transduced with recombinant retroviral vector bearing hepatitis B virus (HBV) core gene and the capability of generating CTLs against HBcAg by genetically modified DCs in vivo.METHODS: A retroviral vector containing HBV core gene was constructed. Replicating DC progenitor of C57BL/6 mice was transduced by retroviral vector and continually cultured in the presence of recombinant mouse granulocytemacrophage colony-stimulating factor (rmGM-CSF) and interleukin-4(IL-4) for 6 days. LPS was added and cultured for additional two days. The efficiency of gene transfer was determined by PCR, Western blot and FACS. Transduced DCs immunized C57BL/6 mice subcutaneously 2 times at an oneweek interval. Intracellular IFN-γ and IL-4 of immunized mice lymphocytes were analyzed. Generation of CTLs in lymphooytes stimulated with mitomycin C-treated EL4-C cell which stably expresses HBcAg was determined by LDH release assays.RESULTS: Recombinant retroviral expression vector (pLCSN) was positively detected by PCR as well as enzyme digestion with EcoRI and BamH I. Retroviruses were generated by pLCSN transfection packing cell and the virus titer was 3x10s CFU/ml. Indirect immunofluorescence and FACS showed that HBV core gene was expressed in murine fibroblasts. Transduced bone marrow cells had capability of differentiating into DCsc in vitro in the presence of rmGMCSF and rmIL-4. The result of PCR showed that HBV core gene was integrated into the genome of transduced DCs.Western blot analysis showed that HBV core gene was expressed in DCs. The transduction rate was 28 % determined by FACS. Retroviral transduction had no influence on DCs expressions of CD80 and MHC class Ⅱ. HBcAg specific CTLs and Th1 type immune responses could be generated in the mice by using transduced DCs as antigen

  10. WT1-specific T cell receptor gene therapy: improving TCR function in transduced T cells.

    Science.gov (United States)

    Stauss, Hans J; Thomas, Sharyn; Cesco-Gaspere, Michela; Hart, Daniel P; Xue, Shao-An; Holler, Angelika; King, Judy; Wright, Graham; Perro, Mario; Pospori, Constantina; Morris, Emma

    2008-01-01

    Adoptive transfer of antigen-specific T lymphocytes is an attractive form of immunotherapy for haematological malignancies and cancer. The difficulty of isolating antigen-specific T lymphocytes for individual patients limits the more widespread use of adoptive T cell therapy. The demonstration that cloned T cell receptor (TCR) genes can be used to produce T lymphocyte populations of desired specificity offers new opportunities for antigen-specific T cell therapy. The first trial in humans demonstrated that TCR gene-modified T cells persisted for an extended time period and reduced tumor burden in some patients. The WT1 protein is an attractive target for immunotherapy of leukemia and solid cancer since elevated expression has been demonstrated in AML, CML, MDS and in breast, colon and ovarian cancer. In the past, we have isolated high avidity CTL specific for a WT1-derived peptide presented by HLA-A2 and cloned the TCR alpha and beta genes of a WT1-specific CTL line. The genes were inserted into retroviral vectors for transduction of human peripheral blood T lymphocytes of leukemia patients and normal donors. The treatment of leukemia-bearing NOD/SCID mice with T cells transduced with the WT1-specific TCR eliminated leukemia cells in the bone marrow of most mice, while treatment with T cells transduced with a TCR of irrelevant specificity did not diminish the leukemia burden. In order to improve the safety and efficacy of TCR gene therapy, we have developed lentiviral TCR gene transfer. In addition, we employed strategies to enhance TCR expression while avoiding TCR mis-pairing. It may be possible to generate dominant TCR constructs that can suppress the expression of the endogenous TCR on the surface of transduced T cells. The development of new TCR gene constructs holds great promise for the safe and effective delivery of TCR gene therapy for the treatment of malignancies. PMID:17855129

  11. Mechanical properties of mammalian single smooth muscle cells. I. A low cost large range microforce transducer.

    NARCIS (Netherlands)

    J.J. Glerum (Jacobus); R. van Mastrigt (Ron)

    1990-01-01

    textabstractA transducer has been developed for measuring the minute forces generated during isometric contractions (1.0-10.0 microN) of single smooth muscle cells from the pig urinary bladder and the human uterus. In addition to its high sensitivity, resolution and stability (100 mV microN-1, and l

  12. EXPRESSION OF rhBMP—7 GENE IN TRANSDUCED BONE MARROW DERIVED STROMAL CELLS

    Institute of Scientific and Technical Information of China (English)

    段德宇; 杜靖远

    2002-01-01

    Objective:To explore the possibility of expression of exogenous gene in transduced bone marrow derived stromal cells(BMSCs).Methods:The marker gene,pbLacZ,was transferred into cultured BMSCs and the expression of transduced gene by x-gal staining was examined.Then plasmid pcDNA3-rhBMP7 was delivered to cultured BMSCs.Through immunohistochemical staining and RT-PCR assay,the expression of rhBMP7 gene was detected.Results:The exogenous gene could be expressed efficiently in transduced BMSCs.Conculsion:The present study provided a theoretical basis to gene therapy on the problems of bone and cartilage tissue.

  13. Recovery of mechano-electrical transduction in rat cochlear hair bundles after postnatal destruction of the stereociliar cross-links

    OpenAIRE

    Ebert, J.; Fink, S.; Koitschev, A; P. Walther; Langer, M G; Lehmann-Horn, F.

    2010-01-01

    Mechano-electrical transduction (MET) in the stereocilia of outer hair cells (OHCs) was studied in newborn Wistar rats using scanning electron microscopy to investigate the stereociliar cross-links, Nomarski laser differential interferometry to investigate stereociliar stiffness and by testing the functionality of the MET channels by recording the entry of fluorescent dye, FM1-43, into stereocilia. Preparations were taken from rats on their day of birth (P0) or 1–4 days later (P1–P4). Hair bu...

  14. Antitumor effect of murine dendritic and tumor cells transduced with IL-2 gene Antitumor effect of murine dendritic and tumor cells transduced with IL-2 gene

    Directory of Open Access Journals (Sweden)

    Justyna Wojas-Turek

    2012-10-01

    Full Text Available Interleukin (IL- 2 acts on a number of types of immune cells promoting their effector functions. To replace
    systemic administration of recombinant form of this cytokine, various genetically modified cells have been used in
    different preclinical models for tumor growth inhibition. In this study, dendritic or tumor cells transduced with retroviral
    vector carrying IL-2 gene (JAWS II/IL-2, X63/IL-2, MC38/IL-2 cells alone or combined with tumor antigenstimulated
    dendritic cells (JAWS II/TAg were exploited to treat colon carcinoma MC38-bearing mice. After the
    peritumoral injection of vaccine cells, the tumor growth delay and the increase in the number of tumor infiltrating
    CD4+ and CD8+ T lymphocytes were noted. A considerable increase in CD4+ cell influx into tumor tissue was observed
    when JAWS II/IL-2 cells or JAWS II/TAg with syngeneic MC38/IL-2 cells were applied. The increase in
    intensity of CD8+ cell infiltration was associated with immune reaction triggered by the same combination of applied
    cells or JAWS II/TAg with allogeneic X63/IL-2 cells. The effect observed in vivo was accompanied by MC38/0 cell
    specific cytotoxic activity of spleen cells in vitro. Thus, the application of vaccines, including IL-2-secreting cells of
    various origins, was able to induce different antitumor responses polarized by exogenous IL-2 and the encountered
    tumor antigen.Interleukin (IL- 2 acts on a number of types of immune cells promoting their effector functions. To replace
    systemic administration of recombinant form of this cytokine, various genetically modified cells have been used in
    different preclinical models for tumor growth inhibition. In this study, dendritic or tumor cells transduced with retroviral
    vector carrying IL-2 gene (JAWS II/IL-2, X63/IL-2, MC38/IL-2 cells alone or combined with tumor antigenstimulated
    dendritic cells (JAWS II/TAg were exploited to treat colon

  15. Autologous Transplantation of Lentivector/Acid Ceramidase–Transduced Hematopoietic Cells in Nonhuman Primates

    OpenAIRE

    Walia, Jagdeep S; Neschadim, Anton; Lopez-Perez, Orlay; Alayoubi, Abdulfatah; Fan, Xin; Carpentier, Stéphane; Madden, Melissa; Lee, Chyan-Jang; Cheung, Fred; Jaffray, David A.; Levade, Thierry; McCart, J Andrea; Jeffrey A Medin

    2011-01-01

    Farber disease is a rare lysosomal storage disorder (LSD) that manifests due to acid ceramidase (AC) deficiencies and ceramide accumulation. We present a preclinical gene therapy study for Farber disease employing a lentiviral vector (LV-huAC/huCD25) in three enzymatically normal nonhuman primates. Autologous, mobilized peripheral blood (PB) cells were transduced and infused into fully myelo-ablated recipients with tracking for at least 1 year. Outcomes were assessed by measuring the AC speci...

  16. Genetic barcode sequencing for screening altered population dynamics of hematopoietic stem cells transduced with lentivirus

    Science.gov (United States)

    Zanatta, Daniela B; Tsujita, Maristela; Borelli, Primavera; Aguiar, Rodrigo B; Ferrari, Daniel G; Strauss, Bryan E

    2014-01-01

    Insertional mutagenesis has been associated with malignant cell transformation in gene therapy protocols, leading to discussions about vector security. Therefore, clonal analysis is important for the assessment of vector safety and its impact on patient health. Here, we report a unique approach to assess dynamic changes in clonality of lentivirus transduced cells upon Sanger sequence analysis of a specially designed genetic barcode. In our approach, changes in the electropherogram peaks are measured and compared between successive time points, revealing alteration in the cell population. After in vitro validation, barcoded lentiviral libraries carrying IL2RG or LMO2 transgenes, or empty vector were used to transduce mouse hematopoietic (ckit+) stem cells, which were subsequently transplanted in recipient mice. We found that neither the empty nor IL2RG encoding vector had an effect on cell dynamics. In sharp contrast, the LMO2 oncogene was associated with altered cell dynamics even though hematologic counts remained unchanged, suggesting that the barcode could reveal changes in cell populations not observed by the frontline clinical assay. We describe a simple and sensitive method for the analysis of clonality, which could be easily used by any laboratory for the assessment of cellular behavior upon lentiviral transduction. PMID:26052520

  17. Safe sorting of GFP-transduced live cells for subsequent culture using a modified FACS vantage

    DEFF Research Database (Denmark)

    Sørensen, T U; Gram, G J; Nielsen, S D;

    1999-01-01

    culture. RESULTS: The bacteriophage sorting showed that the biologically active material was confined to the sorting chamber. A failure mode simulating a nozzle blockage resulted in detectable droplets inside the sorting chamber, but no droplets could be detected when an additional air suction from....... Safety tests with bacteriophages were performed to evaluate the potential spread of biologically active material during cell sorting. Cells transduced with a retroviral vector carrying the gene for GFP were sorted on the basis of their GFP fluorescence, and GFP expression was followed during subsequent...

  18. Dendritic Cells Transduced with SOCS1 Gene Exhibit Regulatory DC Properties and Prolong Allograft Survival

    Institute of Scientific and Technical Information of China (English)

    Hong Fu; Shaohua Song; Fang Liu; Zhijia Ni; Yi Tang; Xiaoyun Shen; Liang Xiao; Guoshan Ding; Quanxing Wang

    2009-01-01

    SOCS1 is a key regulator of cytokine signaling and is important for maintaining balance in the immune system. It is thought to participate in negative feedback loops in cytokine signaling and may be an important signal for the regulation of dendritic cell (DC) maturation. However, it remains unclear whether DCs transduced with SOCS1 exhibit characteristics of regulatory DCs and induce allogeneic T-cell hyporesponsiveness. In this study, we constructed adenoviral vector coding SOCS1 (Ad-SOCS1) that can efficiently increase SOCS1 gene expression in bone marrow-derived dendritic cells. DCs transduced with Ad-SOCS1 (DC-SOCS1) expressed low levels of costimulatory and MHC molecules, were resistant to maturation and activation stimulation, induced allogeneic T-cell hyporesponsiveness, and promoted the generation of regulatory-like T cells in vitro. DC-SOCS1 pretreatment significantly prolonged the survival of allografts and led to a substantial increase in the generation of regulatory T cells. Our data suggest that SOCS1 inhibits DC maturation and induces regulatory DC generation, therefore possessing therapeutic potential to prevent rejection in organ transplantation. Cellular & Molecular Immunology.

  19. Electromechanical transducer for rapid detection, discrimination and quantification of lung cancer cells

    Science.gov (United States)

    Ali, Waqas; Jalvhei Moghaddam, Fatemeh; Usman Raza, Muhammad; Bui, Loan; Sayles, Bailey; Kim, Young-Tae; Iqbal, Samir M.

    2016-05-01

    Tumor cells are malignant derivatives of normal cells. There are characteristic differences in the mechanophysical properties of normal and tumor cells, and these differences stem from the changes that occur in the cell cytoskeleton during cancer progression. There is a need for viable whole blood processing techniques for rapid and reliable tumor cell detection that do not require tagging. Micropore biosensors have previously been used to differentiate tumor cells from normal cells and we have used a micropore-based electromechanical transducer to differentiate one type of tumor cells from the other types. This device generated electrical signals that were characteristic of the cell properties. Three non-small cell lung cancer (NSCLC) cell lines, NCl-H1155, A549 and NCI-H460, were successfully differentiated. NCI-H1155, due to their comparatively smaller size, were found to be the quickest in translocating through the micropore. Their translocation through a 15 μm micropore caused electrical pulses with an average translocation time of 101 ± 9.4 μs and an average peak amplitude of 3.71 ± 0.42 μA, whereas translocation of A549 and NCI-H460 caused pulses with average translocation times of 126 ± 17.9 μs and 148 ± 13.7 μs and average peak amplitudes of 4.58 ± 0.61 μA and 5.27 ± 0.66 μA, respectively. This transformation of the differences in cell properties into differences in the electrical profiles (i.e. the differences in peak amplitudes and translocation times) with this electromechanical transducer is a quantitative way to differentiate these lung cancer cells. The solid-state micropore device processed whole biological samples without any pre-processing requirements and is thus ideal for point-of-care applications.

  20. A FAK-Cas-Rac-lamellipodin signaling module transduces extracellular matrix stiffness into mechanosensitive cell cycling.

    Science.gov (United States)

    Bae, Yong Ho; Mui, Keeley L; Hsu, Bernadette Y; Liu, Shu-Lin; Cretu, Alexandra; Razinia, Ziba; Xu, Tina; Puré, Ellen; Assoian, Richard K

    2014-06-17

    Tissue and extracellular matrix (ECM) stiffness is transduced into intracellular stiffness, signaling, and changes in cellular behavior. Integrins and several of their associated focal adhesion proteins have been implicated in sensing ECM stiffness. We investigated how an initial sensing event is translated into intracellular stiffness and a biologically interpretable signal. We found that a pathway consisting of focal adhesion kinase (FAK), the adaptor protein p130Cas (Cas), and the guanosine triphosphatase Rac selectively transduced ECM stiffness into stable intracellular stiffness, increased the abundance of the cell cycle protein cyclin D1, and promoted S-phase entry. Rac-dependent intracellular stiffening involved its binding partner lamellipodin, a protein that transmits Rac signals to the cytoskeleton during cell migration. Our findings establish that mechanotransduction by a FAK-Cas-Rac-lamellipodin signaling module converts the external information encoded by ECM stiffness into stable intracellular stiffness and mechanosensitive cell cycling. Thus, lamellipodin is important not only in controlling cellular migration but also for regulating the cell cycle in response to mechanical signals.

  1. Using intron splicing trick for preferential gene expression in transduced cells: an approach for suicide gene therapy.

    Science.gov (United States)

    Pourzadegan, F; Shariati, L; Taghizadeh, R; Khanahmad, H; Mohammadi, Z; Tabatabaiefar, M A

    2016-01-01

    Suicide gene therapy is one of the most innovative approaches in which a potential toxic gene is delivered to the targeted cancer cell by different target delivery methods. We constructed a transfer vector to express green fluorescent protein (GFP) in transduced cells but not in packaging cells. We placed gfp under the control of the cytomegalovirus (CMV) promoter, which is positioned between the two long-terminal repeats in reverse direction. The intron-2 sequence of the human beta globin gene with two poly-A signals and several stop codons on the antisense strand was placed on the leading strand between the CMV promoter and gfp. For lentiviral production, the HEK293T and line were co-transfected with the PMD2G, psPAX2 and pLentiGFP-Ins2 plasmids. The HEK293T and line were transduced with this virus. PCR was performed for evaluation of intron splicing in transduced cells. The GFP expression was seen in 65% of the cells transduced. The PCR amplification of the genomic DNA of transduced cells confirmed the splicing of intron 2. The strategy is significant to accomplish our goal for preserving the packaging cells from the toxic gene expression during viral assembly and the resultant reduction in viral titration. Also it serves to address several other issues in the gene therapy.

  2. Various cells retrovirally transduced with N-acetylgalactosoamine-6-sulfate sulfatase correct Morquio skin fibroblasts in vitro.

    Science.gov (United States)

    Toietta, G; Severini, G M; Traversari, C; Tomatsu, S; Sukegawa, K; Fukuda, S; Kondo, N; Tortora, P; Bordignon, C

    2001-11-01

    Gene therapy may provide a long-term approach to the treatment of mucopolysaccharidoses. As a first step toward the development of an effective gene therapy for mucopolysaccharidosis type IVA (Morquio syndrome), a recombinant retroviral vector, LGSN, derived from the LXSN vector, containing a full-length human wildtype N-acetylgalactosamine-6-sulfate sulfatase (GALNS) cDNA, was produced. Severe Morquio and normal donor fibroblasts were transduced by LGSN. GALNS activity in both Morquio and normal transduced cells was several fold higher than normal values. To measure the variability of GALNS expression among different transduced cells, we transduced normal and Morquio lymphoblastoid B cells and PBLs, human keratinocytes, murine myoblasts C2C12, and rabbit synoviocytes HIG-82 with LGSN. In all cases, an increase of GALNS activity after transduction was measured. In Morquio cells co-cultivated with enzyme-deficient transduced cells, we demonstrated enzyme uptake and persistence of GALNS activity above normal levels for up to 6 days. The uptake was mannose-6-phosphate dependent. Furthermore, we achieved clear evidence that LGSN transduction of Morquio fibroblasts led to correction of the metabolic defect. These results provide the first evidence that GALNS may be delivered either locally or systematically by various cells in an ex vivo gene therapy of MPS IVA. PMID:11686941

  3. Leptomeningeal Cells Transduce Peripheral Macrophages Inflammatory Signal to Microglia in Reponse to Porphyromonas gingivalis LPS

    Directory of Open Access Journals (Sweden)

    Yicong Liu

    2013-01-01

    Full Text Available We report here that the leptomeningeal cells transduce inflammatory signals from peripheral macrophages to brain-resident microglia in response to Porphyromonas gingivalis (P.g. LPS. The expression of Toll-like receptor 2 (TLR2, TLR4, TNF-α, and inducible NO synthase was mainly detected in the gingival macrophages of chronic periodontitis patients. In in vitro studies, P.g. LPS induced the secretion of TNF-α and IL-1β from THP-1 human monocyte-like cell line and RAW264.7 mouse macrophages. Surprisingly, the mean mRNA levels of TNF-α and IL-1β in leptomeningeal cells after treatment with the conditioned medium from P.g. LPS-stimulated RAW264.7 macrophages were significantly higher than those after treatment with P.g. LPS alone. Furthermore, the mean mRNA levels of TNF-α and IL-1β in microglia after treatment with the conditioned medium from P.g. LPS-stimulated leptomeningeal cells were significantly higher than those after P.g. LPS alone. These observations suggest that leptomeninges serve as an important route for transducing inflammatory signals from macrophages to microglia by secretion of proinflammatory mediators during chronic periodontitis. Moreover, propolis significantly reduced the P.g. LPS-induced TNF-α and IL-1 β production by leptomeningeal cells through inhibiting the nuclear factor-κB signaling pathway. Together with the inhibitory effect on microglial activation, propolis may be beneficial in preventing neuroinflammation during chronic periodontitis.

  4. Mechano-electric Effect of Hardened Cement Paste During Quasi-static Loading

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    Mechano-electric effect of cement paste was investigated in this paper. As compressive stress was applied on the specimen, an electrical current was observed. The intensity of the electrical current increased with stress increasing, and decreased with stress decreasing. Different measurement methods were also discussed in this paper. This phenomenon was related to the electrokinetic phenomenon of solid/liquid interface in cement paste. The study on mechano-electric effect of hardened cement paste provides a new method for making smart concrete structures.

  5. Human adipose derived mesenchymal stromal cells transduced with GFP lentiviral vectors: assessment of immunophenotype and differentiation capacity in vitro.

    Science.gov (United States)

    van Vollenstee, Fiona A; Jackson, Carlo; Hoffmann, Danie; Potgieter, Marnie; Durandt, Chrisna; Pepper, Michael S

    2016-10-01

    Adipose derived mesenchymal stromal/stem cells (ASCs) are a heterogeneous population characterized by (a) their ability to adhere to plastic; (b) immunophenotypic expression of certain cell surface markers, while lacking others; and (c) the capacity to differentiate into lineages of mesodermal origin including osteocytes, chondrocytes and adipocytes. The long-term goal is to utilize these cells for clinical translation into cell-based therapies. However, preclinical safety and efficacy need to be demonstrated in animal models. ASCs can also be utilized as biological vehicles for vector-based gene delivery systems, since they are believed to home to sites of inflammation and infection in vivo. These factors motivated the development of a labelling system for ASCs using lentiviral vector-based green fluorescent protein (GFP) transduction. Human ASCs were transduced with GFP-expressing lentiviral vectors. A titration study determined the viral titer required to transduce the maximum number of ASCs. The effect of the transduced GFP lentiviral vector on ASC immunophenotypic expression of surface markers as well as their ability to differentiate into osteocytes and adipocytes were assessed in vitro. A transduction efficiency in ASC cultures of approximately 80 % was observed with an MOI of ~118. No significant immunophenotypic differences were observed between transduced and non-transduced cells and both cell types successfully differentiated into adipocytes and osteocytes in vitro. We obtained >80 % transduction of ASCs using GFP lentiviral vectors. Transduced ASCs maintained plastic adherence, demonstrated ASC immunophenotype and the ability to differentiate into cells of the mesodermal lineage. This GFP-ASC transduction technique offers a potential tracking system for future pre-clinical studies.

  6. A novel gene delivery method transduces porcine pancreatic duct epithelial cells.

    Science.gov (United States)

    Griffin, M A; Restrepo, M S; Abu-El-Haija, M; Wallen, T; Buchanan, E; Rokhlina, T; Chen, Y H; McCray, P B; Davidson, B L; Divekar, A; Uc, A

    2014-02-01

    Gene therapy offers the possibility to treat pancreatic disease in cystic fibrosis (CF), caused by mutations in the CF transmembrane conductance regulator (CFTR) gene; however, gene transfer to the pancreas is untested in humans. The pancreatic disease phenotype is very similar between humans and pigs with CF; thus, CF pigs create an excellent opportunity to study gene transfer to the pancreas. There are no studies showing efficient transduction of pig pancreas with gene-transfer vectors. Our objective is to develop a safe and efficient method to transduce wild-type (WT) porcine pancreatic ducts that express CFTR. We catheterized the umbilical artery of WT newborn pigs and delivered an adeno-associated virus serotype 9 vector expressing green-fluorescent protein (AAV9CMV.sceGFP) or vehicle to the celiac artery, the vessel that supplies major branches to the pancreas. This technique resulted in stable and dose-dependent transduction of pancreatic duct epithelial cells that expressed CFTR. Intravenous (IV) injection of AAV9CMV.sceGFP did not transduce the pancreas. Our technique offers an opportunity to deliver the CFTR gene to the pancreas of CF pigs. The celiac artery can be accessed via the umbilical artery in newborns and via the femoral artery at older ages--delivery approaches that can be translated to humans. PMID:24257348

  7. Transduced Tat-DJ-1 protein inhibits cytokines-induced pancreatic RINm5F cell death

    Science.gov (United States)

    Jo, Hyo Sang; Yeo, Hyeon Ji; Cha, Hyun Ju; Kim, Sang Jin; Cho, Su Bin; Park, Jung Hwan; Lee, Chi Hern; Yeo, Eun Ji; Choi, Yeon Joo; Eum, Won Sik; Choi, Soo Young

    2016-01-01

    Loss of pancreatic β-cells by oxidative stress or cytokines is associated with diabetes mellitus (DM). DJ-1 is known to as a multifunctional protein, which plays an important role in cell survival. We prepared cell permeable wild type (WT) and mutant type (M26I) Tat-DJ-1 proteins to investigate the effects of DJ-1 against combined cytokines (IL-1β, IFN-γ and TNF-α)-induced RINm5F cell death. Both Tat-DJ-1 proteins were transduced into RINm5F cells. WT Tat-DJ-1 proteins significantly protected against cell death from cytokines by reducing intracellular toxicities. Also, WT Tat-DJ-1 proteins markedly regulated cytokines-induced pro- and anti-apoptosis proteins. However, M26I Tat-DJ-1 protein showed relatively low protective effects, as compared to WT Tat-DJ-1 protein. Our experiments demonstrated that WT Tat-DJ-1 protein protects against cytokine-induced RINm5F cell death by suppressing intracellular toxicities and regulating apoptosisrelated protein expression. Thus, WT Tat-DJ-1 protein could potentially serve as a therapeutic agent for DM and cytokine related diseases. [BMB Reports 2016; 49(5): 297-302] PMID:26996344

  8. Numerical analysis of acoustic impedance microscope utilizing acoustic lens transducer to examine cultured cells.

    Science.gov (United States)

    Gunawan, Agus Indra; Hozumi, Naohiro; Takahashi, Kenta; Yoshida, Sachiko; Saijo, Yoshifumi; Kobayashi, Kazuto; Yamamoto, Seiji

    2015-12-01

    A new technique is proposed for non-contact quantitative cell observation using focused ultrasonic waves. This technique interprets acoustic reflection intensity into the characteristic acoustic impedance of the biological cell. The cells are cultured on a plastic film substrate. A focused acoustic beam is transmitted through the substrate to its interface with the cell. A two-dimensional (2-D) reflection intensity profile is obtained by scanning the focal point along the interface. A reference substance is observed under the same conditions. These two reflections are compared and interpreted into the characteristic acoustic impedance of the cell based on a calibration curve that was created prior to the observation. To create the calibration curve, a numerical analysis of the sound field is performed using Fourier Transforms and is verified using several saline solutions. Because the cells are suspended by two plastic films, no contamination is introduced during the observation. In a practical observation, a sapphire lens transducer with a center frequency of 300 MHz was employed using ZnO thin film. The objects studied were co-cultured rat-derived glial (astrocyte) cells and glioma cells. The result was the clear observation of the internal structure of the cells. The acoustic impedance of the cells was spreading between 1.62 and 1.72 MNs/m(3). Cytoskeleton was indicated by high acoustic impedance. The introduction of cytochalasin-B led to a significant reduction in the acoustic impedance of the glioma cells; its effect on the glial cells was less significant. It is believed that this non-contact observation method will be useful for continuous cell inspections.

  9. Suprathreshold Stochastic Resonance in Mechanoelectrical Transduction of Hair Cells

    Institute of Scientific and Technical Information of China (English)

    ZHAO Xiang-Hui; LONG Zhang-Cai; ZHANG Bing; YANG Nan

    2008-01-01

    @@ Haircell, the sound signal sensor in the auditory system, works in an environment full of noise. This noise has been demonstrated before to be a factor beneficial to the transfer of subthreshold weak signal. We further theoretically demonstrate that in suprathreshold signal situation, noise can still be a beneficial factor and enhance the halrcell nonlinear transfer of suprathreshold signal. This reveals an additional constructive function of noise in hearing and provides theoretic foundation for relevant applications.

  10. Novel Two-Dimensional Mechano-Electric Generators and Sensors Based on Transition Metal Dichalcogenides

    Science.gov (United States)

    Yu, Sheng; Eshun, Kwesi; Zhu, Hao; Li, Qiliang

    2015-08-01

    Transition metal dichalcogenides (TMDCs), such as MoS2 and WSe2, provide two-dimensional atomic crystals with semiconductor band gap. In this work, we present a design of new mechano-electric generators and sensors based on transition metal dichalcogenide nanoribbon PN junctions and heterojunctions. The mechano-electric conversion was simulated by using a first-principle calculation. The output voltage of MoS2 nanoribbon PN junction increases with strain, reaching 0.036 V at 1% strain and 0.31 V at 8% strain, much larger than the reported results. Our study indicates that the length, width and layer number of TMDC nanoribbon PN junctions have an interesting but different impact on the voltage output. Also, the results indicate that doping position and concentration only cause a small fluctuation in the output voltage. These results have been compared with the mechano-electric conversion of TMDC heterojunctions. Such novel mechano-electric generators and sensors are very attractive for applications in future self-powered, wearable electronics and systems.

  11. Adoptive transfer of syngeneic T cells transduced with a chimeric antigen receptor that recognizes murine CD19 can eradicate lymphoma and normal B cells

    OpenAIRE

    Kochenderfer, James N.; Yu, Zhiya; Frasheri, Dorina; Restifo, Nicholas P; Rosenberg, Steven A.

    2010-01-01

    Adoptive T-cell therapy with anti-CD19 chimeric antigen receptor (CAR)–expressing T cells is a new approach for treating advanced B-cell malignancies. To evaluate anti-CD19–CAR-transduced T cells in a murine model of adoptive T-cell therapy, we developed a CAR that specifically recognized murine CD19. We used T cells that were retrovirally transduced with this CAR to treat mice bearing a syngeneic lymphoma that naturally expressed the self-antigen murine CD19. One infusion of anti-CD19–CAR-tr...

  12. Functional and genomic analyses of FOXP3-transduced Jurkat-T cells as regulatory T (Treg)-like cells

    International Nuclear Information System (INIS)

    FOXP3, a forkhead transcription factor is essential for the development and function of CD4+CD25+ regulatory T cells (Tregs). In contrast to conversion of murine naive T cells to Tregs by transduction of Foxp3, it is controversial whether ectopic expression of FOXP3 in human CD4+ T cells is sufficient for acquisition of suppressive activity. Here, we show that retroviral transduction of FOXP3 induces a Treg phenotype in human leukemic CD4+ Jurkat-T cells, evidenced by increased expression of Treg-associated cell surface markers as well as inhibition of cytokine production. Furthermore, FOXP3-transduced Jurkat-T cells suppress the proliferation of human CD4+CD25- T cells. Additionally, DNA microarray analysis identifies Treg-related genes regulated by FOXP3. Our study demonstrates that enforced expression of FOXP3 confers Treg-like properties on Jurkat-T cells, which can be a convenient and efficient Treg-like cell model for further study to identify Treg cell surface markers and target genes regulated by FOXP3

  13. Thin-film transducers for the detection and imaging of Brillouin oscillations in transmission on cultured cells

    Science.gov (United States)

    Pérez-Cota, F.; Smith, R. J.; Moradi, E.; Webb, K.; Clark, M.

    2016-01-01

    Mechanical imaging and characterisation of biological cells has been a subject of interest for the last twenty years. Ultrasonic imaging based on the scanning acoustic microscope (SAM) and mechanical probing have been extensively reported. Large acoustic attenuation at high frequencies and the use of conventional piezo-electric transducers limit the operational frequency of a SAM. This limitation results in lower resolution compared to an optical microscope. Direct mechanical probing in the form of applied stress by contacting probes causes stress to cells and exhibits poor depth resolution. More recently, laser ultrasound has been reported to detect ultrasound in the GHz range via Brillouin oscillations on biological cells. This technique offers a promising new high resolution acoustic cell imaging technique. In this work, we propose, design and apply a thin-film based opto-acoustic transducer for the detection in transmission of Brillouin oscillations on cells. The transducer is used to generate acoustic waves, protect the cells from laser radiation and enhance signal-to-noise ratio (SNR). Experimental traces are presented in water films as well as images of the Brillouin frequency of phantom and fixed 3T3 fibroblast cells.

  14. Efficient expression of histidine-tagged large hepatitis delta antigen in baculovirus-transduced baby hamster kidney cells

    Institute of Scientific and Technical Information of China (English)

    Ying-Wei Chiang; Jaw-Chin Wu; Kuei-Chun Wang; Chia-Wei Lai; Yao-Chi Chung; Yu-Chen Hu

    2006-01-01

    AIM: To study the baculovirus/mammalian cell system for efficient expression of functional large hepatitis delta antigen (L-HDAg).METHODS: A recombinant baculovirus expressing histidine-tagged L-HDAg (L-HDAgH) was constructed to transduce baby hamster kidney (BHK) cells by a simplified transduction protocol.RESULTS: The recombinant baculovirus transduced BHK cells with efficiencies higher than 90% as determined by flow cytometry. The expression level was significantly higher than that obtained by plasmid transfection and was further enhanced 3-fold to around 19 pg/cell by the addition of 10 mmol/L sodium butyrate. Importantly,the expressed L-HDAgH was localized to the cell nucleus and correctly isoprenylated as determined by immunofluorescence labeling and confocal microscopy.Moreover, L-HDAgH interacted with hepatitis B surface antigen to form virus-like particles.CONCLUSION: The fusion with histidine tags as well as overexpression of L-HDAgH in the baculovirus-transduced BHK cells does not impair the biological functions. Taken together, the baculovirus/mammalian cell system offers an attractive alternative for high level expression of L-HDAgH or other proteins that require extensive posttranslational modifications.

  15. DNA Methylation and Histone Modifications Are the Molecular Lock in Lentivirally Transduced Hematopoietic Progenitor Cells

    Directory of Open Access Journals (Sweden)

    Siew Ching Ngai

    2015-01-01

    Full Text Available Stable introduction of a functional gene in hematopoietic progenitor cells (HPCs has appeared to be an alternative approach to correct genetically linked blood diseases. However, it is still unclear whether lentiviral vector (LV is subjected to gene silencing in HPCs. Here, we show that LV carrying green fluorescent protein (GFP reporter gene driven by cytomegalovirus (CMV promoter was subjected to transgene silencing after transduction into HPCs. This phenomenon was not due to the deletion of proviral copy number. Study using DNA demethylating agent and histone deacetylase (HDAC inhibitor showed that the drugs could either prevent or reverse the silencing effect. Using sodium bisulfite sequencing and chromatin immunoprecipitation (ChIP assay, we demonstrated that DNA methylation occurred soon after LV transduction. At the highest level of gene expression, CMV promoter was acetylated and was in a euchromatin state, while GFP reporter gene was acetylated but was strangely in a heterochromatin state. When the expression declined, CMV promoter underwent transition from acetylated and euchromatic state to a heterochromatic state, while the GFP reporter gene was in deacetylated and heterochromatic state. With these, we verify that DNA methylation and dynamic histone modifications lead to transgene silencing in HPCs transduced with LV.

  16. B7-1/CD80-transduced tumor cells elicit better systemic immunity than wild-type tumor cells admixed with Corynebacterium parvum.

    Science.gov (United States)

    Chen, L; McGowan, P; Ashe, S; Johnston, J V; Hellström, I; Hellström, K E

    1994-10-15

    Tumor cells genetically modified by transduction of B7 (B7-1/CD80), a natural ligand for the T-cell costimulatory molecules CD28 and CTLA-4, can elicit potent tumor immunity, and they can be effective for treatment of established cancers in animal models. In this study, three tumor lines, the EL4 lymphoma, the P815 mastocytoma, and the MCA102 sarcoma were transduced with recombinant retrovirus containing the murine B7 gene, and their potency to induce systemic immunity protective against challenge with wild-type tumor was compared to that of the same tumor cells admixed with the commonly used adjuvant Corynebacterium parvum. While admixture of tumor cells with C. parvum resulted in complete regression of tumors in syngeneic mice, it did not induce protective immunity against a subsequent challenge of wild-type cells from any of the 3 tumors tested. In contrast, B7-transduced EL4 and P815 tumors regressed locally and induced a potent systemic immunity to wild-type tumors and a higher level of cytotoxic T-cell activity than did tumor cells admixed with C. parvum. No systemic immunity was induced by B7-transduced nonimmunogenic MCA102 sarcoma cells. Our results demonstrate that immunogenic tumor cells transduced with the B7 gene are superior to tumor cells mixed with C. parvum for the induction of systemic tumor immunity. PMID:7522958

  17. Withaferin A inhibits activation of signal transducer and activator of transcription 3 in human breast cancer cells

    OpenAIRE

    Lee, Joomin; Hahm, Eun-Ryeong; Singh, Shivendra V

    2010-01-01

    We have shown previously that withaferin A (WA), a promising anticancer constituent of Ayurvedic medicine plant Withania somnifera, inhibits growth of human breast cancer cells in culture and in vivo in association with apoptosis induction. The present study builds on these observations and demonstrates that WA inhibits constitutive as well as interleukin-6 (IL-6)-inducible activation of signal transducer and activator of transcription 3 (STAT3), which is an oncogenic transcription factor act...

  18. Mesenchymal stem cells transduced by PLEGFP-N1 retroviral vector maintain their biological features and differentiation

    Institute of Scientific and Technical Information of China (English)

    HE Xu; LI Yu-lin; WANG Xin-rui; GUO Xin; NIU Yun

    2005-01-01

    Methods hMSCs were isolated from human bone marrow by density gradient fractionation and adherence to plastic flasks. Individual colonies were selected and cultured in tissue dishes. Packaging cells PT67 were transfected by PLEGFP-N1 retroviral vector , and hMSCs were transduced by viral supernatant infection. Meanwhile, hMSCs-EGFP were identified by immune phenotypes and whether it could differentiate into osteoblasts or adipocytes under conditioned media was investigated. Results The rate of stably transduced hMSCs-EGFP was up to 96% after being screened by G418. hMSCs-EGFP exhibited fibroblast-like morphological features. Flow cytometric analyses showed that hMSCs-EGFP were positive for CD73, CD105, CD166, CD90 and CD44, but negative for CD34 and CD45. In addition, it could functionally be induced into osteocytes or adipocytes under conditioned media. These biological features of hMSCs-EGFP were consistent with those of hMSCs.Conclusions hMSCs transduced by PLEGFP-N1 retroviral vector can be used in vivo securely because they can maintain their biological characteristics and differentiation. It is a simple and reliable way to trace the changes of hMSCs in vivo by EGFP during cell transplantation and gene therapy.

  19. Ultrasonic transducer

    International Nuclear Information System (INIS)

    A description is given of an ultrasonic transducer capable of operating at high temperature and comprising a transducer crystal and a coupling piece. This coupling piece is composed of several thin plates, generally triangular in shape, in a material withstanding corrosion and high temperatures, these plates being applied one against the other by pressure. One of the edges of the coupling piece is designed so as to direct towards the junction surfaces of the various plates the ultrasonic waves reflected from the junction between the coupling piece and the piece to which the ultrasonic waves must be transmitted

  20. Long term follow up of patients after allogeneic stem cell transplantation and transfusion of HSV-Tk transduced T-cells.

    Directory of Open Access Journals (Sweden)

    Eva Maria Weissinger

    2015-04-01

    Full Text Available Allogeneic stem cell transplantation (allo-HSCT is one of the curative treatments for hematologic malignancies, but is hampered by severe complications, such as acute or chronic graft-versus-host-disease (aGvHD; cGvHD and infections. CD34-selcetion of stem cells reduces the risk of aGvHD, but also leads to increased infectious complications and relapse. Thus, we studied the efficacy, safety and feasibility of transfer of gene modified donor T-cells shortly after allo-HSCT in two clinical trials between 2002 and 2007 and here we compare the results to unmodified donor leukocyte transfusion (DLI. The aim of these trials was to provide patients with the protection of T-cells after T-cell-depleted allo-HSCT in the matched or mismatched donor setting with an option to delete transduced T-cells, if severe aGvHD occurred within the trial period. Donor-T-cells were transduced with the replication-deficient retrovirus SFCMM-3, expressing HSV-Tk and the truncated LNGFR for selection of transduced cells. Transduced cells were transfused either after day +60 (matched donors or on day +42 (haploidentical donors.Nine patients were included in the first trial (MHH; 2002 until 2007 2 were included in TK007 (2005-2009 and 6 serve as a control group for outcome after haploidentical transplantation without HSV-TK-transduced DLI. Three patients developed acute GvHD, two had grade I of the skin, one had aGvHD on day +131 (post-HSCT; +89 post-HSV-Tk DLI grade II, which was successfully controlled by ganciclovir (GCV. Donor chimerism was stabilized after transfusion of the transduced cells in all patients treated. Functionality of HSV-Tk gene expressing T-cells was shown by loss of bcr-abl gene expression as well as by control of cytomegalovirus-reactivation. To date, 6patients have relapsed and died, 2 after a second HSCT without T-cell depletion or administration of unmodified T-cells. Eleven patients (7 post-HSV-Tk DLI are alive and well to date.

  1. An effective vaccine against colon cancer in mice: Use of recombinant adenovirus interleukin-12 transduced dendritic cells

    Institute of Scientific and Technical Information of China (English)

    Xiao-Zhou He; Liang Wang; Yan-Yun Zhang

    2008-01-01

    AIM: To investigate the effect of a vaccine with recombinant adenovirus interleukin-12 (AdVIL-12) transduced dendritic cells (DCs) against colon cancer in mice.METHODS: DCs and AdVIL-12 were incubated together at different time intervals and at different doses. Supernatant was collected and tested for IL-12 by enzyme-linked immunosorbent assay (ELISA). In order to determine whether tumor cell lysate-pulsed (TP) AdVIL-12/DCs enhance therapeutic potential in the established tumor model, CT26 colon tumor cells were implanted subcutaneously (s.c.) in the midflank of naive BALB/c mice. Tumor-bearing mice were injected with a vaccination of CT26 TP AdVIL-12/DCs on d 3 and 10. As a protective colon tumor model, na(i)ve BALB/c mice were immunized s.c. in their abdomens with CT26 TP AdVIL-12/DCs twice at seven day intervals. After the immunization on d 7, the mice were challenged with a lethal dose of CT26 tumor cells and survival times were evaluated. Subsequently, cytotoxic T lymphocyte (CTL) activity and interferon gamma (IFNγ) secretion was evaluated in the immunized mice, and assayed CTL ex vivo.RESULTS: Murine DCs were retrovirally transduced with AdVIL-12 efficiency, and the AdVIL-12 transduced DCs secreted a high level of IL-12 (AdVIL-12/DCs, 615.27 ± 42.3 pg/mL vs DCs, 46.32 ± 7.29 pg/mL, P < 0.05). Vaccination with CT26 TP AdVIL-12/DCs could enhance anti-tumor immunity against CT26 colon tumor in murine therapeutic models (tumor volume on d 19: CT26 TP AdVIL-12/DCs 107 ± 42 mm3 vsCT26 TP DCs 383 ± 65 mm3, P < 0.05) and protective models. Moreover, the CT26 TP AdVIL-12/DC vaccination enhances tumor-specific CTL activity, producing high levels of IFNy in immunized mice. Ex vivo primed T cells with AdVIL-12/DCs were able to induce more effective CTL activity than in primed T cells with CT26 TP/DCs (E:T = 100:1, 69.49% ± 6.11% specific lysis vs 37.44% ± 4.32% specific lysis, P < 0.05).CONCLUSION: Vaccination with recombinant AdVIL-12 transduced DC pulsed tumor

  2. Ultrasonic transducer

    International Nuclear Information System (INIS)

    An ultrasonic transducer suitable for use up to a temperature of about 6000C comprises a stainless steel casing containing a lithium niobate piezoelectric element and a backing material of a powder which provides a partial pressure of oxygen and thereby prevents deterioration of the element by oxygen loss or contamination. The powder might be of lithium niobate or magnesia. (author)

  3. Recovery of mechano-electrical transduction in rat cochlear hair bundles after postnatal destruction of the stereociliar cross-links.

    Science.gov (United States)

    Ebert, J; Fink, S; Koitschev, A; Walther, P; Langer, M G; Lehmann-Horn, F

    2010-08-01

    Mechano-electrical transduction (MET) in the stereocilia of outer hair cells (OHCs) was studied in newborn Wistar rats using scanning electron microscopy to investigate the stereociliar cross-links, Nomarski laser differential interferometry to investigate stereociliar stiffness and by testing the functionality of the MET channels by recording the entry of fluorescent dye, FM1-43, into stereocilia. Preparations were taken from rats on their day of birth (P0) or 1-4 days later (P1-P4). Hair bundles developed from the base to the apex and from the inner to outer OHC rows. MET channel responses were detected in apical coil OHCs on P1. To study the possible recovery of MET after disrupting the cross-links, the same investigations were performed after the application of Ca(2+) chelator 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA) and allowing the treated samples to recover in culture medium for 0-20 h. We found that the structure and function were abolished by BAPTA. In P0-P1 samples, structural recovery was complete and the open probability of MET channels reached control values. In P3-P4 samples, complete recovery only occurred in OHCs of the outermost row. Although our results demonstrate an enormous recovery potential of OHCs in the postnatal period, the structural component restricts the potential for therapy in patients. PMID:20356889

  4. Mechano-electrical transduction : New insights into old ideas

    NARCIS (Netherlands)

    Ricci, A. J.; Kachar, B.; Gale, J.; Van Netten, S. M.

    2006-01-01

    The gating-spring theory of hair cell mechanotransduction channel activation was first postulated over twenty years ago. The basic tenets of this hypothesis have been reaffirmed in hair cells from both auditory and vestibular systems and across species. In fact, the basic findings have been reproduc

  5. Investigation of the Mechanoelectrical Transduction at Single Stereocilia by Afm

    Science.gov (United States)

    Langer, M. G.; Fink, S.; Löffler, K.; Koitschev, A.; Zenner, H.-P.

    2003-02-01

    The transduction of sound into an electrical signal in the inner ear is closely related to the mechanical properties of the hair bundles cytoskeleton and cross-linkage. In this study the effect of lateral cross-links on hair bundle mechanics and the transduction current response is demonstrated on the level of individual stereocilia. For experiments stereocilia of outer hair cells of postnatal rats (P3 - P8) were scanned with a sharp AFM tip at nanometerscale. Transduction currents were simultaneously recorded in the whole-cell-recording mode with patch clamp. AFM was used as a nanotool for local mechanical stimulation and force measurement at stereocilia whereas patch clamp serves as a detector for the electrical response of the cell. In a first experiment force transmission between adjacent stereocilia of the V- and W- shaped hair bundles of outer hair cells was investigated. Results showed that a force exerted to a single stereocilium declined to 36 % at the nearest adjacent stereocilium of the same row. This result supposes AFM to be convenient for local displacement of single stereocilia. For control, the local response of transduction channels was measured at single stereocilia of the same hair bundle. Measured transduction current amplitudes ranged from 9 to 49 pA supposing an opening of one to five transduction channels. Both, weak force transmission by lateral cross-links and small transduction current amplitudes indicate a weak mechanical interaction between individual stereocilia of the tallest row of stereocilia of outer hair cells from postnatal rats.

  6. Kurarinol induces hepatocellular carcinoma cell apoptosis through suppressing cellular signal transducer and activator of transcription 3 signaling

    Energy Technology Data Exchange (ETDEWEB)

    Shu, Guangwen; Yang, Jing; Zhao, Wenhao; Xu, Chan; Hong, Zongguo; Mei, Zhinan; Yang, Xinzhou, E-mail: xinzhou_yang@hotmail.com

    2014-12-01

    Kurarinol is a flavonoid isolated from roots of the medical plant Sophora flavescens. However, its cytotoxic activity against hepatocellular carcinoma (HCC) cells and toxic effects on mammalians remain largely unexplored. Here, the pro-apoptotic activities of kurarinol on HCC cells and its toxic impacts on tumor-bearing mice were evaluated. The molecular mechanisms underlying kurarinol-induced HCC cell apoptosis were also investigated. We found that kurarinol dose-dependently provoked HepG2, Huh-7 and H22 HCC cell apoptosis. In addition, kurarinol gave rise to a considerable decrease in the transcriptional activity of signal transducer and activator of transcription 3 (STAT3) in HCC cells. Suppression of STAT3 signaling is involved in kurarinol-induced HCC cell apoptosis. In vivo studies showed that kurarinol injection substantially induced transplanted H22 cell apoptosis with low toxic impacts on tumor-bearing mice. Similarly, the transcriptional activity of STAT3 in transplanted tumor tissues was significantly suppressed after kurarinol treatment. Collectively, our current research demonstrated that kurarinol has the capacity of inducing HCC cell apoptosis both in vitro and in vivo with undetectable toxic impacts on the host. Suppressing STAT3 signaling is implicated in kurarinol-mediated HCC cell apoptosis. - Highlights: • Kurarinol induces hepatocellular carcinoma (HCC) cell apoptosis. • Kurarinol induces HCC cell apoptosis via inhibiting STAT3. • Kurarinol exhibits low toxic effects on tumor-bearing animals.

  7. Kallikrein transduced mesenchymal stem cells protect against anti-GBM disease and lupus nephritis by ameliorating inflammation and oxidative stress.

    Directory of Open Access Journals (Sweden)

    Yajuan Li

    Full Text Available Previously we have shown that kallikreins (klks play a renoprotective role in nephrotoxic serum induced nephritis. In this study, we have used mesenchymal stem cells (MSCs as vehicles to deliver klks into the injured kidneys and have measured their therapeutic effect on experimental antibody induced nephritis and lupus nephritis. Human KLK-1 (hKLK1 gene was transduced into murine MSCs using a retroviral vector to generate a stable cell line, hKLK1-MSC, expressing high levels of hKLK1. 129/svj mice subjected to anti-GBM induced nephritis were transplanted with 10(6 hKLK1-MSCs and hKLK1 expression was confirmed in the kidneys. Compared with vector-MSCs injected mice, the hKLK1-MSCs treated mice showed significantly reduced proteinuria, blood urea nitrogen (BUN and ameliorated renal pathology. Using the same strategy, we treated lupus-prone B6.Sle1.Sle3 bicongenic mice with hKLK1-MSCs and demonstrated that hKLK1-MSCs delivery also attenuated lupus nephritis. Mechanistically, hKLK1-MSCs reduced macrophage and T-lymphocyte infiltration into the kidney by suppressing the expression of inflammation cytokines. Moreover, hKLK1 transduced MSCs were more resistant to oxidative stress-induced apoptosis. These findings advance genetically modified MSCs as potential gene delivery tools for targeting therapeutic agents to the kidneys in order to modulate inflammation and oxidative stress in lupus nephritis.

  8. Adeno-associated virus type 2 (AAV2) capsid-specific cytotoxic T lymphocytes eliminate only vector-transduced cells coexpressing the AAV2 capsid in vivo.

    Science.gov (United States)

    Li, Chengwen; Hirsch, Matthew; Asokan, Aravind; Zeithaml, Brian; Ma, Hong; Kafri, Tal; Samulski, R Jude

    2007-07-01

    A recent clinical trial has suggested that recombinant adeno-associated virus (rAAV) vector transduction in humans induces a cytotoxic T-lymphocyte (CTL) response against the AAV2 capsid. To directly address the ability of AAV capsid-specific CTLs to eliminate rAAV-transduced cells in vitro and in vivo in mice, we first demonstrated that AAV2 capsid-specific CTLs could be induced by dendritic cells with endogenous AAV2 capsid expression or pulsed with AAV2 vectors. These CTLs were able to kill a cell line stable for capsid expression in vitro and also in a mouse tumor xenograft model in vivo. Parent colon carcinoma (CT26) cells transduced with a large amount of AAV2 vectors in vitro were also destroyed by these CTLs. To determine the effect of CTLs on the elimination of target cells transduced by AAV2 vectors in vivo, we carried out adoptive transfer experiments. CTLs eliminated liver cells with endogenous AAV2 capsid expression but not liver cells transduced by AAV2 vectors, regardless of the reporter genes. Similar results were obtained for rAAV2 transduction in muscle. Our data strongly suggest that AAV vector-transduced cells are rarely eliminated by AAV2 capsid-specific CTLs in vivo, even though the AAV capsid can induce a CTL response. In conclusion, AAV capsid-specific CTLs do not appear to play a role in elimination of rAAV-transduced cells in a mouse model. In addition, our data suggest that the mouse model may not mimic the immune response noted in humans and additional modification to AAV vectors may be required for further study in order to elicit a similar cellular immune response.

  9. Adeno-Associated Virus Type 2 (AAV2) Capsid-Specific Cytotoxic T Lymphocytes Eliminate Only Vector-Transduced Cells Coexpressing the AAV2 Capsid In Vivo▿

    Science.gov (United States)

    Li, Chengwen; Hirsch, Matthew; Asokan, Aravind; Zeithaml, Brian; Ma, Hong; Kafri, Tal; Samulski, R. Jude

    2007-01-01

    A recent clinical trial has suggested that recombinant adeno-associated virus (rAAV) vector transduction in humans induces a cytotoxic T-lymphocyte (CTL) response against the AAV2 capsid. To directly address the ability of AAV capsid-specific CTLs to eliminate rAAV-transduced cells in vitro and in vivo in mice, we first demonstrated that AAV2 capsid-specific CTLs could be induced by dendritic cells with endogenous AAV2 capsid expression or pulsed with AAV2 vectors. These CTLs were able to kill a cell line stable for capsid expression in vitro and also in a mouse tumor xenograft model in vivo. Parent colon carcinoma (CT26) cells transduced with a large amount of AAV2 vectors in vitro were also destroyed by these CTLs. To determine the effect of CTLs on the elimination of target cells transduced by AAV2 vectors in vivo, we carried out adoptive transfer experiments. CTLs eliminated liver cells with endogenous AAV2 capsid expression but not liver cells transduced by AAV2 vectors, regardless of the reporter genes. Similar results were obtained for rAAV2 transduction in muscle. Our data strongly suggest that AAV vector-transduced cells are rarely eliminated by AAV2 capsid-specific CTLs in vivo, even though the AAV capsid can induce a CTL response. In conclusion, AAV capsid-specific CTLs do not appear to play a role in elimination of rAAV-transduced cells in a mouse model. In addition, our data suggest that the mouse model may not mimic the immune response noted in humans and additional modification to AAV vectors may be required for further study in order to elicit a similar cellular immune response. PMID:17475652

  10. Signal transducer and activator of transcription 3 activation is associated with bladder cancer cell growth and survival

    Directory of Open Access Journals (Sweden)

    Hsieh Fu-Chuan

    2008-10-01

    Full Text Available Abstract Background Constitutive activation of signal transducer and activator of transcription 3 (Stat3 signaling pathway plays an important role in several human cancers. Activation of Stat3 is dependent on the phosphorylation at the tyrosine residue 705 by upstream kinases and subsequent nuclear translocation after dimerization. It remains unclear whether oncogenic Stat3 signaling pathway is involved in the oncogenesis of bladder cancer. Results We found that elevated Stat3 phosphorylation in 19 of 100 (19% bladder cancer tissues as well as bladder cancer cell lines, WH, UMUC-3 and 253J. To explore whether Stat3 activation is associated with cell growth and survival of bladder cancer, we targeted the Stat3 signaling pathway in bladder cancer cells using an adenovirus-mediated dominant-negative Stat3 (Y705F and a small molecule compound, STA-21. Both prohibited cell growth and induction of apoptosis in these bladder cancer cell lines but not in normal bladder smooth muscle cell (BdSMC. The survival inhibition might be mediated through apoptotic caspase 3, 8 and 9 pathways. Moreover, down-regulation of anti-apoptotic genes (Bcl-2, Bcl-xL and survivin and a cell cycle regulating gene (cyclin D1 was associated with the cell growth inhibition and apoptosis. Conclusion These results indicated that activation of Stat3 is crucial for bladder cancer cell growth and survival. Therefore, interference of Stat3 signaling pathway emerges as a potential therapeutic approach for bladder cancer.

  11. Fabrication and integration of permanent magnet materials into MEMS transducers

    Science.gov (United States)

    Wang, Naigang

    Microscale permanent magnets (PM) are a key building block for magnetically based microelectromechanical systems (MEMS), such as sensors, actuators, and energy converters. However, the inability to concurrently achieve good magnetic properties and an integrated magnet fabrication process hinders the development of magnetic MEMS. To address this need, this dissertation develops methods for wafer-level microfabrication of thick (10--500+ microm), high-performance, permanent magnets using low-temperature (electrodynamic transducer prototypes. A cantilever-type microtransducer achieves a 2.7 microm vertical deflection at a driving current of 5.5 mArms at 100 Hz. A piston-type transducer with elastomeric membrane obtains a 2.2 microm vertical displacement at a driving current of 670 mArms at 200 Hz. These devices demonstrate the integrability of wax-bonded Nd-Fe-B powder magnets into microscale electromechanical transducers. Electromechanical lumped element models are then developed for the piston-type electrodynamic actuators. The models enable prediction of the device performance as an electroacoustic actuator (microspeaker) and as a mechanoelectrical generator (vibrational energy harvester). Then, both the acoustic and energy harvesting performance of the prototype transducers are experimentally measured to verify the LEM models. The validated models provide a design tool for further design and development of these types of micromagnetic MEMS devices.

  12. Anti-inflammatory effect of transduced PEP-1-heme oxygenase-1 in Raw 264.7 cells and a mouse edema model

    Energy Technology Data Exchange (ETDEWEB)

    Kwon, Soon Won; Sohn, Eun Jeong; Kim, Dae Won; Jeong, Hoon Jae; Kim, Mi Jin; Ahn, Eun Hee; Kim, Young Nam; Dutta, Suman [Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702 (Korea, Republic of); Kim, Duk-Soo [Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan-Si 330-090 (Korea, Republic of); Park, Jinseu; Eum, Won Sik [Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702 (Korea, Republic of); Hwang, Hyun Sook, E-mail: wazzup@hallym.ac.kr [Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702 (Korea, Republic of); Choi, Soo Young, E-mail: sychoi@hallym.ac.kr [Department of Biomedical Science and Research Institute of Bioscience and Biotechnology, Hallym University, Chunchon 200-702 (Korea, Republic of)

    2011-07-29

    Highlights: {yields} Recombinant PEP-1 heme oxygenase-1 expression vector was constructed and overexpressed. {yields} We investigated transduction efficiency of PEP-1-HO-1 protein in Raw 264.7 cells. {yields} PEP-1-HO-1 was efficiently transduced into Raw 264.7 cells in a dose and time dependent manner. {yields} PEP-1-HO-1 exerted anti-inflammatory activity in Raw 264.7 cells and in a mice edema model. {yields} PEP-1-HO-1 could be used as a therapeutic drug against inflammatory diseases. -- Abstract: Heme oxygenase-1 (HO-1), which catalyzes the degradation of free heme to biliverdin, carbon monoxide (CO), and free iron (Fe{sup 2+}), is up-regulated by several cellular stress and cell injuries, including inflammation, ischemia and hypoxia. In this study, we examined whether fusion of HO-1 with PEP-1, a protein transduction domain that is able to deliver exogenous molecules to living cells or tissues, would facilitate HO-1 delivery to target cells and tissues, and thereby effectively exert a therapeutically useful response against inflammation. Western blot analysis demonstrated that PEP-1-HO-1 fusion proteins were transduced into Raw 264.7 cells in time- and dose-dependent manners, and were stably maintained in the cells for about 60 h. In addition, fluorescence analysis revealed that only PEP-1-HO-1 fusion proteins were significantly transduced into the cytoplasm of cells, while HO-1 proteins failed to be transduced. In lipopolysaccharide (LPS)-stimulated Raw 264.7 cells and 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse edema model, transduced PEP-1-HO-1 fusion proteins effectively inhibited the overexpression of pro-inflammatory mediators and cytokines. Also, histological analysis demonstrated that PEP-1-HO-1 remarkably suppressed ear edema. The results suggest that the PEP-1-HO-1 fusion protein can be used as a therapeutic molecule against reactive oxygen species-related inflammatory diseases.

  13. Transplantation of ATP7B-transduced bone marrow mesenchymal stem cells decreases copper overload in rats.

    Directory of Open Access Journals (Sweden)

    Shenglin Chen

    Full Text Available BACKGROUND: Recent studies have demonstrated that transplantation of ATP7B-transduced hepatocytes ameliorates disease progression in LEC (Long-Evans Cinnamon rats, a model of Wilson's disease (WD. However, the inability of transplanted cells to proliferate in a normal liver hampers long-term treatment. In the current study, we investigated whether transplantation of ATP7B-transduced bone marrow mesenchymal stem cells (BM-MSCs could decrease copper overload in LEC rats. MATERIALS AND METHODS: The livers of LEC rats were preconditioned with radiation (RT and/or ischemia-reperfusion (IRP before portal vein infusion of ATP7B-transduced MSCs (MSCsATP7B. The volumes of MSCsATP7B or saline injected as controls were identical. The expression of ATP7B was analyzed by real-time quantitative polymerase chain reaction (RT-PCR at 4, 12 and 24 weeks post-transplantation. MSCATP7B repopulation, liver copper concentrations, serum ceruloplasmin levels, and alanine transaminase (ALT and aspartate transaminase (AST levels were also analyzed at each time-point post-transplantation. RESULTS: IRP-plus-RT preconditioning was the most effective strategy for enhancing the engraftment and repopulation of transplanted MSCsATP7B. This strategy resulted in higher ATP7B expression and serum ceruloplasmin, and lower copper concentration in this doubly preconditioned group compared with the saline control group, the IRP group, and the RT group at all three time-points post-transplantation (p<0.05 for all. Moreover, 24 weeks post-transplantation, the levels of ALT and AST in the IRP group, the RT group, and the IRP-plus-RT group were all significantly decreased compared to those of the saline group (p<0.05 compared with the IRP group and RT group, p<0.01 compared with IRP-plus-RT group; ALT and AST levels were significantly lower in the IRP-plus-RT group compared to either the IRP group or the RT group (p<0.01 and p<0.05. respectively. CONCLUSIONS: These results demonstrate

  14. Ethanol Metabolism by HeLa Cells Transduced with Human Alcohol Dehydrogenase Isoenzymes: Control of the Pathway by Acetaldehyde Concentration†

    Science.gov (United States)

    Matsumoto, Michinaga; Cyganek, Izabela; Sanghani, Paresh C.; Cho, Won Kyoo; Liangpunsakul, Suthat; Crabb, David W.

    2010-01-01

    Background Human class I alcohol dehydrogenase 2 isoenzymes (encoded by the ADH1B locus) have large differences in kinetic properties; however, individuals inheriting the alleles for the different isoenzymes exhibit only small differences in alcohol elimination rates. This suggests that other cellular factors must regulate the activity of the isoenzymes. Methods The activity of the isoenzymes expressed from ADH1B*1, ADH1B*2, and ADH1B*3 cDNAs was examined in stably transduced HeLa cell lines, including lines which expressed human low Km aldehyde dehydrogenase (ALDH2). The ability of the cells to metabolize ethanol was compared with that of HeLa cells expressing rat class I ADH (HeLa-rat ADH cells), rat hepatoma (H4IIEC3) cells, and rat hepatocytes. Results The isoenzymes had similar protein half-lives in the HeLa cells. Rat hepatocytes, H4IIEC3 cells, and HeLa-rat ADH cells oxidized ethanol much faster than the cells expressing the ADH1B isoenzymes. This was not explained by high cellular NADH levels or endogenous inhibitors; but rather because the activity of the β1 and β2 ADHs were constrained by the accumulation of acetaldehyde, as shown by the increased rate of ethanol oxidation by cell lines expressing β2 ADH plus ALDH2. Conclusion The activity of the human β2 ADH isoenzyme is sensitive to inhibition by acetaldehyde, which likely limits its activity in vivo. This study emphasizes the importance of maintaining a low steady–state acetaldehyde concentration in hepatocytes during ethanol metabolism. PMID:21166830

  15. Cocultivation of umbilical cord blood CD34+ cells with retro-transduced hMSCs leads to effective amplification of long-term culture-initiating cells

    Institute of Scientific and Technical Information of China (English)

    Chun-Gang Xie; Jin-Fu Wang; Ying Xiang; Li-Yan Qiu; Bing-Bing Jia; Li-Juan Wang; Guo-Zhong Wang; Guo-Ping Huang

    2006-01-01

    AIM: To establish a novel coculture system for ex vivo expansion of umbilical cord blood(UCB) hematopoietic progenitors using thrombopoietin (TPO)/Flt-3 ligand(FL)-transduced human marrow-derived mesenchymal stem cells (tfhMSCs) as feeder.METHODS: UCB CD34+ cells were isolated and cultured using four culture systems in serum-containing or serumfree medium. Suitable aliquots of cultured cells were used to monitor cell production, clonogenic activity,and long-term culture-initiating culture (LTC-IC) output.Finally, the severe-combined immunodeficient (SCID)mouse-repopulating cell (SRC) assay was performed to confirm ability of the cultured cells to reconstitute longterm hematopoiesis.RESULTS: There were no significant differences in the number of total nucleated cells among different culture systems in serum-containing medium during 21-d culture. However, on d 14, the outputs of CD34+ cells,CFU-C and CFU-GEMM in tfhMSCs coculture system were significantly enhanced. LTC-IC assay demonstrated that the tfhMSCs coculture system had the most powerful activity. The severe-combined immunodeficient (SCID)mouse repopulating cell (SRC) assay confirmed extensive ability of the expanded cells to reconstitute long-term hematopoiesis. Furthermore, PCR analysis demonstrated the presence of human hematopoietic cells in the bone marrow and peripheral blood cells of NOD/SCID mice.CONCLUSION: The TPO/FL-transduced hMSCs, in combination with additive cytokines, can effectively expand hematopoietic progenitors from UCB in vitro and the tfhMSCs coculture system may be a suitable system for ex vivo manipulation of primitive progenitor cells under contact culture conditions.

  16. Driving electrostatic transducers

    DEFF Research Database (Denmark)

    Nielsen, Dennis; Knott, Arnold; Andersen, Michael A. E.

    2013-01-01

    Electrostatic transducers represent a very interesting alternative to the traditional inefficient electrodynamic transducers. In order to establish the full potential of these transducers, power amplifiers which fulfill the strict requirements imposed by such loads (high impedance, frequency...

  17. VP22 herpes simplex virus protein can transduce proteins into stem cells

    International Nuclear Information System (INIS)

    The type I herpes simplex virus VP22 tegument protein is abundant and well known for its ability to translocate proteins from one cell to the other. In spite of some reports questioning its ability to translocate proteins by attributing the results observed to fixation artifacts or simple attachment to the cell membrane, VP22 has been used to deliver several proteins into different cell types, triggering the expected cell response. However, the question of the ability of VP22 to enter stem cells has not been addressed. We investigated whether VP22 could be used as a tool to be applied in stem cell research and differentiation due to its capacity to internalize other proteins without altering the cell genome. We generated a VP22.eGFP construct to evaluate whether VP22 could be internalized and carry another protein with it into two different types of stem cells, namely adult human dental pulp stem cells and mouse embryonic stem cells. We generated a VP22.eGFP fusion protein and demonstrated that, in fact, it enters stem cells. Therefore, this system may be used as a tool to deliver various proteins into stem cells, allowing stem cell research, differentiation and the generation of induced pluripotent stem cells in the absence of genome alterations

  18. VP22 herpes simplex virus protein can transduce proteins into stem cells

    Energy Technology Data Exchange (ETDEWEB)

    Gabanyi, I.; Lojudice, F.H.; Kossugue, P.M. [Centro de Terapia Celular e Molecular, Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP (Brazil); Rebelato, E. [Departamento de Fisiologia e Biofísica, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP (Brazil); Demasi, M.A.; Sogayar, M.C. [Centro de Terapia Celular e Molecular, Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, São Paulo, SP (Brazil)

    2013-02-01

    The type I herpes simplex virus VP22 tegument protein is abundant and well known for its ability to translocate proteins from one cell to the other. In spite of some reports questioning its ability to translocate proteins by attributing the results observed to fixation artifacts or simple attachment to the cell membrane, VP22 has been used to deliver several proteins into different cell types, triggering the expected cell response. However, the question of the ability of VP22 to enter stem cells has not been addressed. We investigated whether VP22 could be used as a tool to be applied in stem cell research and differentiation due to its capacity to internalize other proteins without altering the cell genome. We generated a VP22.eGFP construct to evaluate whether VP22 could be internalized and carry another protein with it into two different types of stem cells, namely adult human dental pulp stem cells and mouse embryonic stem cells. We generated a VP22.eGFP fusion protein and demonstrated that, in fact, it enters stem cells. Therefore, this system may be used as a tool to deliver various proteins into stem cells, allowing stem cell research, differentiation and the generation of induced pluripotent stem cells in the absence of genome alterations.

  19. High-Pass Filtering at Vestibular Frequencies by Transducer Adaptation in Mammalian Saccular Hair Cells

    Science.gov (United States)

    Songer, Jocelyn E.; Eatock, Ruth Anne

    2011-11-01

    The mammalian saccule detects head tilt and low-frequency head accelerations as well as higher-frequency bone vibrations and sounds. It has two different hair cell types, I and II, dispersed throughout two morphologically distinct regions, the striola and extrastriola. Afferents from the two zones have distinct response dynamics which may arise partly from zonal differences in hair cell properties. We find that type II hair cells in the rat saccular epithelium adapt with a time course appropriate for influencing afferent responses to head motions. Moreover, striolar type II hair cells adapted by a greater extent than extrastriolar type II hair cells and had greater phase leads in the mid-frequency range (5-50 Hz). These differences suggest that hair cell transduction may contribute to zonal differences in the adaptation of vestibular afferents to head motions.

  20. Efficient conditional gene expression following transplantation of retrovirally transduced bone marrow stem cells.

    Science.gov (United States)

    Chung, Jie-Yu; Mackay, Fabienne; Alderuccio, Frank

    2015-01-01

    Retroviral gene therapy combined with bone marrow stem cell transplantation can be used to generate mice with ectopic gene expression in the bone marrow compartment in a quick and cost effective manner when compared to generating and maintaining transgenic mouse lines. However a limitation of this procedure is the lack of cell specificity in gene expression that is associated with the use of endogenous retroviral promoters. Restricting gene expression to specific cell subsets utilising tissue-specific promoter driven retroviral vectors is a challenge. Here we describe the generation of conditional expression of retrovirally encoded genes in specific bone marrow derived cell lineages utilising a Cre-dependent retroviral vector. By utilising Lck and CD19 restricted Cre transgenic bone marrow stem cells, we generate chimeric animals with T or B lymphocyte restricted gene expression respectively. The design of the Cre-dependent retroviral vector enables expression of encoded MOG and GFP genes only in association with Cre mediated DNA inversion. Importantly this strategy does not significantly increase the size of the retroviral vector; as such we are able to generate bone marrow chimeric animals with significantly higher chimerism levels than previous studies utilising Cre-dependent retroviral vectors and Cre transgenic bone marrow stem cells. This demonstrates that the use of Cre-dependent retroviral vectors is able to yield high chimerism levels for experimental use and represent a viable alternative to generating transgenic animals.

  1. Efficient conditional gene expression following transplantation of retrovirally transduced bone marrow stem cells.

    Science.gov (United States)

    Chung, Jie-Yu; Mackay, Fabienne; Alderuccio, Frank

    2015-01-01

    Retroviral gene therapy combined with bone marrow stem cell transplantation can be used to generate mice with ectopic gene expression in the bone marrow compartment in a quick and cost effective manner when compared to generating and maintaining transgenic mouse lines. However a limitation of this procedure is the lack of cell specificity in gene expression that is associated with the use of endogenous retroviral promoters. Restricting gene expression to specific cell subsets utilising tissue-specific promoter driven retroviral vectors is a challenge. Here we describe the generation of conditional expression of retrovirally encoded genes in specific bone marrow derived cell lineages utilising a Cre-dependent retroviral vector. By utilising Lck and CD19 restricted Cre transgenic bone marrow stem cells, we generate chimeric animals with T or B lymphocyte restricted gene expression respectively. The design of the Cre-dependent retroviral vector enables expression of encoded MOG and GFP genes only in association with Cre mediated DNA inversion. Importantly this strategy does not significantly increase the size of the retroviral vector; as such we are able to generate bone marrow chimeric animals with significantly higher chimerism levels than previous studies utilising Cre-dependent retroviral vectors and Cre transgenic bone marrow stem cells. This demonstrates that the use of Cre-dependent retroviral vectors is able to yield high chimerism levels for experimental use and represent a viable alternative to generating transgenic animals. PMID:25445328

  2. Stable Marking and Transgene Expression Without Progression to Monoclonality in Canine Long-Term Hematopoietic Repopulating Cells Transduced with Lentiviral Vectors

    OpenAIRE

    Enssle, Joerg; Trobridge, Grant D.; Keyser, Kirsten A.; Ironside, Christina; Beard, Brian C; Kiem, Hans-Peter

    2010-01-01

    Lentiviral gene transfer vectors have a number of potential advantages over gammaretroviral vectors including more efficient transduction of nondividing cells, a more favorable integration site profile, and the ability to accommodate large transgenes. Here, we present long-term follow-up data of animals that received lentivirus-transduced CD34-enriched cells. Six long-term surviving dogs were available for analysis. Transgene expression was analyzed from at least 12 months to more than 5 year...

  3. Suppression of human lung cancer cell proliferation and metastasis in vitro by the transducer of ErbB-2.1 (TOB1)

    OpenAIRE

    Jiao, Yang; SUN, KE-KANG; Zhao, Lin; Xu, Jia-Ying; Wang, Li-Li; Fan, Sai-Jun

    2011-01-01

    Aim: To investigate the effects of the transducer of ErbB-2.1 (TOB1) on the proliferation, migration and invasion of human lung cancer cells in vitro. Methods: Human lung cancer cell lines (95-D, A549, NCI-H1299, NCI-H1975, NCI-H661, NCI-H446, NCI-H1395, and Calu-3) and the normal human bronchial epithelial (HBE) cell line were tested. The expression levels of TOB1 in the cells were determined with Western blot and RT-PCR analyses. TOB1-overexpressing cell line 95-D/TOB1 was constructed using...

  4. Hepatitis C virus-induced myeloid-derived suppressor cells regulate T-cell differentiation and function via the signal transducer and activator of transcription 3 pathway.

    Science.gov (United States)

    Ren, Jun P; Zhao, Juan; Dai, Jun; Griffin, Jeddidiah W D; Wang, Ling; Wu, Xiao Y; Morrison, Zheng D; Li, Guang Y; El Gazzar, Mohamed; Ning, Shun B; Moorman, Jonathan P; Yao, Zhi Q

    2016-08-01

    T cells play a pivotal role in controlling viral infection; however, the precise mechanisms responsible for regulating T-cell differentiation and function during infections are incompletely understood. In this study, we demonstrated an expansion of myeloid-derived suppressor cells (MDSCs), in particular the monocytic MDSCs (M-MDSCs; CD14(+) CD33(+) CD11b(+) HLA-DR(-/low) ), in patients with chronic hepatitis C virus (HCV) infection. Notably, HCV-induced M-MDSCs express high levels of phosphorylated signal transducer and activator of transcription 3 (pSTAT3) and interleukin-10 (IL-10) compared with healthy subjects. Blocking STAT3 signalling reduced HCV-mediated M-MDSC expansion and decreased IL-10 expression. Importantly, we observed a significant increase in the numbers of CD4(+) CD25(+) Foxp3(+) regulatory T (Treg) cells following incubation of healthy peripheral blood mononuclear cells (PBMCs) with MDSCs derived from HCV-infected patients or treated with HCV core protein. In addition, depletion of MDSCs from PBMCs led to a significant reduction of Foxp3(+) Treg cells developed during chronic HCV infection. Moreover, depletion of MDSCs from PBMCs significantly increased interferon-γ production by CD4(+) T effector (Teff) cells derived from HCV patients. These results suggest that HCV-induced MDSCs promote Treg cell development and inhibit Teff cell function, suggesting a novel mechanism for T-cell regulation and a new strategy for immunotherapy against human viral diseases. PMID:27149428

  5. Expression of Galpha14 in sweet-transducing taste cells of the posterior tongue

    Directory of Open Access Journals (Sweden)

    Kim Soochong

    2008-11-01

    Full Text Available Abstract Background "Type II"/Receptor cells express G protein-coupled receptors (GPCRs for sweet, umami (T1Rs and mGluRs or bitter (T2Rs, as well as the proteins for downstream signalling cascades. Transduction downstream of T1Rs and T2Rs relies on G-protein and PLCβ2-mediated release of stored Ca2+. Whereas Gαgus (gustducin couples to the T2R (bitter receptors, which Gα-subunit couples to the sweet (T1R2 + T1R3 receptor is presently not known. We utilized RT-PCR, immunocytochemistry and single-cell gene expression profiling to examine the expression of the Gαq family (q, 11, 14 in mouse taste buds. Results By RT-PCR, Gα14 is expressed strongly and in a taste selective manner in posterior (vallate and foliate, but not anterior (fungiform and palate taste fields. Gαq and Gα11, although detectable, are not expressed in a taste-selective fashion. Further, expression of Gα14 mRNA is limited to Type II/Receptor cells in taste buds. Immunocytochemistry on vallate papillae using a broad Gαq family antiserum reveals specific staining only in Type II taste cells (i.e. those expressing TrpM5 and PLCβ2. This staining persists in Gαq knockout mice and immunostaining with a Gα11-specific antiserum shows no immunoreactivity in taste buds. Taken together, these data show that Gα14 is the dominant Gαq family member detected. Immunoreactivity for Gα14 strongly correlates with expression of T1R3, the taste receptor subunit present in taste cells responsive to either umami or sweet. Single cell gene expression profiling confirms a tight correlation between the expression of Gα14 and both T1R2 and T1R3, the receptor combination that forms sweet taste receptors. Conclusion Gα14 is co-expressed with the sweet taste receptor in posterior tongue, although not in anterior tongue. Thus, sweet taste transduction may rely on different downstream transduction elements in posterior and anterior taste fields.

  6. The Molecular Interaction of CAR and JAML Recruits the Central Cell Signal Transducer PI3K

    Energy Technology Data Exchange (ETDEWEB)

    Verdino, Petra; Witherden, Deborah A.; Havran, Wendy L.; Wilson, Ian A. (Scripps)

    2010-11-15

    Coxsackie and adenovirus receptor (CAR) is the primary cellular receptor for group B coxsackieviruses and most adenovirus serotypes and plays a crucial role in adenoviral gene therapy. Recent discovery of the interaction between junctional adhesion molecule-like protein (JAML) and CAR uncovered important functional roles in immunity, inflammation, and tissue homeostasis. Crystal structures of JAML ectodomain (2.2 angstroms) and its complex with CAR (2.8 angstroms) reveal an unusual immunoglobulin-domain assembly for JAML and a charged interface that confers high specificity. Biochemical and mutagenesis studies illustrate how CAR-mediated clustering of JAML recruits phosphoinositide 3-kinase (P13K) to a JAML intracellular sequence motif as delineated for the {alpha}{beta} T cell costimulatory receptor CD28. Thus, CAR and JAML are cell signaling receptors of the immune system with implications for asthma, cancer, and chronic nonhealing wounds.

  7. Stress Induced Mechano-electrical Writing-Reading of Polymer Film Powered by Contact Electrification Mechanism

    Science.gov (United States)

    Goswami, Sumita; Nandy, Suman; Calmeiro, Tomás R.; Igreja, Rui; Martins, Rodrigo; Fortunato, Elvira

    2016-01-01

    Mechano-electrical writing and reading in polyaniline (PANI) thin film are demonstrated via metal-polymer contact electrification mechanism (CEM). An innovative conception for a non-destructive self-powered writable-readable data sheet is presented which can pave the way towards new type of stress induced current harvesting devices. A localized forced deformation of the interface has been enacted by pressing the atomic force microscopic probe against the polymer surface, allowing charge transfer between materials interfaces. The process yields a well-defined charge pattern by transmuting mechanical stress in to readable information. The average of output current increment has been influenced from 0.5 nA to 15 nA for the applied force of 2 nN to 14 nN instead of electrical bias. These results underscore the importance of stress-induced current harvesting mechanism and could be scaled up for charge patterning of polymer surface to writable-readable data sheet. Time evolutional current distribution (TECD) study of the stress-induced patterned PANI surface shows the response of readability of the recorded data with time.

  8. Intestinal Epithelial Cell Tyrosine Kinase 2 Transduces IL-22 Signals To Protect from Acute Colitis.

    Science.gov (United States)

    Hainzl, Eva; Stockinger, Silvia; Rauch, Isabella; Heider, Susanne; Berry, David; Lassnig, Caroline; Schwab, Clarissa; Rosebrock, Felix; Milinovich, Gabriel; Schlederer, Michaela; Wagner, Michael; Schleper, Christa; Loy, Alexander; Urich, Tim; Kenner, Lukas; Han, Xiaonan; Decker, Thomas; Strobl, Birgit; Müller, Mathias

    2015-11-15

    In the intestinal tract, IL-22 activates STAT3 to promote intestinal epithelial cell (IEC) homeostasis and tissue healing. The mechanism has remained obscure, but we demonstrate that IL-22 acts via tyrosine kinase 2 (Tyk2), a member of the Jak family. Using a mouse model for colitis, we show that Tyk2 deficiency is associated with an altered composition of the gut microbiota and exacerbates inflammatory bowel disease. Colitic Tyk2(-/-) mice have less p-STAT3 in colon tissue and their IECs proliferate less efficiently. Tyk2-deficient primary IECs show reduced p-STAT3 in response to IL-22 stimulation, and expression of IL-22-STAT3 target genes is reduced in IECs from healthy and colitic Tyk2(-/-) mice. Experiments with conditional Tyk2(-/-) mice reveal that IEC-specific depletion of Tyk2 aggravates colitis. Disease symptoms can be alleviated by administering high doses of rIL-22-Fc, indicating that Tyk2 deficiency can be rescued via the IL-22 receptor complex. The pivotal function of Tyk2 in IL-22-dependent colitis was confirmed in Citrobacter rodentium-induced disease. Thus, Tyk2 protects against acute colitis in part by amplifying inflammation-induced epithelial IL-22 signaling to STAT3. PMID:26432894

  9. In vivo safety and persistence of endoribonuclease gene-transduced CD4+ T cells in cynomolgus macaques for HIV-1 gene therapy model.

    Directory of Open Access Journals (Sweden)

    Hideto Chono

    Full Text Available BACKGROUND: MazF is an endoribonuclease encoded by Escherichia coli that specifically cleaves the ACA sequence of mRNA. In our previous report, conditional expression of MazF in the HIV-1 LTR rendered CD4+ T lymphocytes resistant to HIV-1 replication. In this study, we examined the in vivo safety and persistence of MazF-transduced cynomolgus macaque CD4+ T cells infused into autologous monkeys. METHODOLOGY/PRINCIPAL FINDINGS: The in vivo persistence of the gene-modified CD4+ T cells in the peripheral blood was monitored for more than half a year using quantitative real-time PCR and flow cytometry, followed by experimental autopsy in order to examine the safety and distribution pattern of the infused cells in several organs. Although the levels of the MazF-transduced CD4+ T cells gradually decreased in the peripheral blood, they were clearly detected throughout the experimental period. Moreover, the infused cells were detected in the distal lymphoid tissues, such as several lymph nodes and the spleen. Histopathological analyses of tissues revealed that there were no lesions related to the infused gene modified cells. Antibodies against MazF were not detected. These data suggest the safety and the low immunogenicity of MazF-transduced CD4+ T cells. Finally, gene modified cells harvested from the monkey more than half a year post-infusion suppressed the replication of SHIV 89.6P. CONCLUSIONS/SIGNIFICANCE: The long-term persistence, safety and continuous HIV replication resistance of the mazF gene-modified CD4+ T cells in the non-human primate model suggests that autologous transplantation of mazF gene-modified cells is an attractive strategy for HIV gene therapy.

  10. Helicobacter pylori Inhibits Dendritic Cell Maturation via Interleukin-10-Mediated Activation of the Signal Transducer and Activator of Transcription 3 Pathway.

    OpenAIRE

    Rizzuti, David; Ang, Michelle; Sokollik, Christiane; Wu, Ted; Abdullah, Majd; Greenfield, Laura; Fattouh, Ramzi; Reardon, Colin; Tang, Michael; Diao, Jun; Schindler, Christian; Cattral, Mark; Jones, Nicola L

    2014-01-01

    Helicobacter pylori infects the human gastric mucosa causing a chronic infection that is the primary risk factor for gastric cancer development. Recent studies demonstrate that H. pylori promotes tolerogenic dendritic cell (DC) development indicating that this bacterium evades the host immune response. However, the signaling pathways involved in modulating DC activation during infection remain unclear. Here, we report that H. pylori infection activated the signal transducer and activator of t...

  11. The use of SHP-2 gene transduced bone marrow mesenchymal stem cells to promote osteogenic differentiation and bone defect repair in rat.

    Science.gov (United States)

    Fan, Dapeng; Liu, Shen; Jiang, Shichao; Li, Zhiwei; Mo, Xiumei; Ruan, Hongjiang; Zou, Gang-Ming; Fan, Cunyi

    2016-08-01

    Bone tissue engineering is a promising approach for bone regeneration, in which growth factors play an important role. The tyrosine phosphatase Src-homology region 2-containing protein tyrosine phosphatase 2 (SHP2), encoded by the PTPN11 gene, is essential for the differentiation, proliferation and metabolism of osteoblasts. However, SHP-2 has never been systematically studied for its effect in osteogenesis. We predicted that overexpression of SHP-2 could promote bone marrow-derived mesenchymal stem cell (BMSC)osteogenic differentiation and SHP-2 transduced BMSCs could enhance new bone formation, determined using the following study groups: (1) BMSCs transduced with SHP-2 and induced with osteoblast-inducing liquid (BMSCs/SHP-2/OL); (2) BMSCs transduced with SHP-2 (BMSCs/-SHP-2); (3) BMSCs induced with osteoblast-inducing liquid (BMSCs/OL) and (4) pure BMSCs. Cells were assessed for osteogenic differentiation by quantitative real-time polymerase chain reaction analysis, western blot analysis, alkaline phosphatase activity and alizarin red S staining. For in vivo assessment, cells were combined with beta-tricalcium phosphate scaffolds and transplanted into rat calvarial defects for 8 weeks. Following euthanasia, skull samples were explanted for osteogenic evaluation, including micro-computed tomography measurement, histology and immunohistochemistry staining. SHP-2 and upregulation of its gene promoted BMSC osteogenic differentiation and therefore represents a potential new therapeutic approach to bone repair. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1871-1881, 2016. PMID:26999642

  12. Peroxiredoxin 1 functions as a signal peroxidase to receive, transduce, and transmit peroxide signals in mammalian cells.

    Science.gov (United States)

    Jarvis, Reagan M; Hughes, Stephanie M; Ledgerwood, Elizabeth C

    2012-10-01

    Hydrogen peroxide is widely viewed as the main second messenger in redox signaling, and it has been proposed that deactivation of the antioxidant peroxiredoxin (Prdx) enzymes allows free peroxide to accumulate and directly oxidize target proteins (the floodgate model). We assessed the role of cytosolic Prdxs 1 and 2 in peroxide-induced activation of the apoptosis signaling kinase 1 (ASK1)/p38 signaling pathway, in which oxidation of ASK1 is required for phosphorylation of p38. In response to peroxide, Prdx1 catalyzed oxidation of ASK1 to a disulfide-linked multimer, and this occurred via transient formation of a Prdx1-ASK1 mixed disulfide intermediate. Oxidation of ASK1 and phosphorylation of p38 were inhibited by knockdown of Prdx1, but also by overexpression of Prdx2. This suggests that these two cytosolic Prdxs have distinct roles in the cellular peroxide response and compete for available peroxide substrate. These data imply that Prdx1 can function as a peroxide receptor in response to extracellular H(2)O(2), receiving the peroxide signal and transducing it into a disulfide bond that is subsequently transmitted to the substrate, ASK1, resulting in p38 phosphorylation. Interestingly, in response to peroxide, Prdx1 and Prdx3 transiently formed reducible higher molecular weight complexes, suggesting that multiple proteins are targets for Prdx-mediated oxidation via a disulfide-exchange mechanism. This model of active peroxide signal distribution via disulfide exchange is consistent with Prdx function in yeast and explains how peroxides may trigger specific disulfide bond formation in mammalian cells.

  13. Coincident signals from GPCRs and receptor tyrosine kinases are uniquely transduced by PI3Kβ in myeloid cells.

    Science.gov (United States)

    Houslay, Daniel M; Anderson, Karen E; Chessa, Tamara; Kulkarni, Suhasini; Fritsch, Ralph; Downward, Julian; Backer, Jonathan M; Stephens, Len R; Hawkins, Phillip T

    2016-01-01

    Class I phosphoinositide 3-kinases (PI3Ks) catalyze production of the lipid messenger phosphatidylinositol 3,4,5-trisphosphate (PIP3), which plays a central role in a complex signaling network regulating cell growth, survival, and movement. This network is overactivated in cancer and inflammation, and there is interest in determining the PI3K catalytic subunit (p110α, p110β, p110γ, or p110δ) that should be targeted in different therapeutic contexts. Previous studies have defined unique regulatory inputs for p110β, including direct interaction with Gβγ subunits, Rac, and Rab5. We generated mice with knock-in mutations of p110β that selectively blocked the interaction with Gβγ and investigated its contribution to the PI3K isoform dependency of receptor tyrosine kinase (RTK) and G protein (heterotrimeric guanine nucleotide-binding protein)-coupled receptor (GPCR) responses in primary macrophages and neutrophils. We discovered a unique role for p110β in supporting synergistic PIP3 formation in response to the coactivation of macrophages by macrophage colony-stimulating factor (M-CSF) and the complement protein C5a. In contrast, we found partially redundant roles for p110α, p110β, and p110δ downstream of M-CSF alone and a nonredundant role for p110γ downstream of C5a alone. This role for p110β completely depended on direct interaction with Gβγ, suggesting that p110β transduces GPCR signals in the context of coincident activation by an RTK. The p110β-Gβγ interaction was also required for neutrophils to generate reactive oxygen species in response to the Fcγ receptor-dependent recognition of immune complexes and for their β2 integrin-mediated adhesion to fibrinogen or poly-RGD+, directly implicating heterotrimeric G proteins in these two responses. PMID:27531651

  14. Handbook of force transducers

    CERN Document Server

    Stefanescu, Dan Mihai

    2011-01-01

    Part I introduces the basic ""Principles and Methods of Force Measurement"" acording to a classification into a dozen of force transducers types: resistive, inductive, capacitive, piezoelectric, electromagnetic, electrodynamic, magnetoelastic, galvanomagnetic (Hall-effect), vibrating wires, (micro)resonators, acoustic and gyroscopic. Two special chapters refer to force balance techniques and to combined methods in force measurement. Part II discusses the ""(Strain Gauge) Force Transducers Components"", evolving from the classical force transducer to the digital / intelligent one, with the inco

  15. Inhibition of signal transducer and activator of transcription 3 expression by RNA interference suppresses invasion through inducing anoikis in human colon cancer cells

    Institute of Scientific and Technical Information of China (English)

    Yu Fan; You-Li Zhang; Ying Wu; Wei Zhang; Yin-Huan Wang; Zhao-Ming Cheng; Hua Li

    2008-01-01

    AIM: To investigate the roles and mechanism of signal transducer and activator of transcription 3 (STAT3) in invasion of human colon cancer cells by RNA interference. METHODS: Small interfering RNA (siRNA) targeting Signal transducer and activator of transcription 3 (STAT3) was transfected into HT29 colon cancer cells. STAT3 protein level and DNA-binding activity of STAT3 was evaluated by western blotting and electrophoretic mobility shift assay (EMSA), respectively. We studied the anchorage-independent growth using colony formation in soft agar, and invasion using the boyden chamber model, anoikis using DNA fragmentation assay and terminal deoxynucleotidyltransferase-mediated dUTP nick-end labeling (TUNEL), respectively. Western blot assay was used to observe the protein expression of Bcl-xL and survivin in colon cancer HT29 cells. RESULTS: RNA interference (RNAi) mediated by siRNA leads to suppression of STAT3 expression in colon cancer cell lines. Suppression of STAT3 expression by siRNA could inhibit anchorage-independent growth, and invasion ability, and induces anoikis in the colon cancer cell line HT29. It has been shown that knockdown of STAT3 expression by siRNA results in a reduction in expression of Bcl-xL and survivin in HT29 cells. CONCLUSION: These results suggest that STAT3 siRNA can inhibit the invasion ability of colon cancer cells through inducing anoikis, which antiapoptotic genes survivin and Bcl-xL contribute to regulation of anoikis. These studies indicate STAT3 siRNA could be a useful therapeutic tool for the treatment of colon cancer.

  16. Crossflow force transducer

    International Nuclear Information System (INIS)

    A force transducer for measuring lift and drag coefficients for a circular cylinder in turbulent water flow is presented. In addition to describing the actual design and construction of the strain-gauged force- ring based transducer, requirements for obtained valid fluid force test data are discussed, and pertinent flow test experience is related

  17. Modeling of ultrasound transducers

    DEFF Research Database (Denmark)

    Bæk, David

    This Ph.D. dissertation addresses ultrasound transducer modeling for medical ultrasound imaging and combines the modeling with the ultrasound simulation program Field II. The project firstly presents two new models for spatial impulse responses (SIR)s to a rectangular elevation focused transducer...

  18. Mitochondrial translocation of signal transducer and activator of transcription 5 (STAT5) in leukemic T cells and cytokine-stimulated cells

    Energy Technology Data Exchange (ETDEWEB)

    Chueh, Fu-Yu; Leong, King-Fu [Department of Microbiology and Immunology, H. M. Bligh Cancer Research Laboratories, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064 (United States); Yu, Chao-Lan, E-mail: chaolan.yu@rosalindfranklin.edu [Department of Microbiology and Immunology, H. M. Bligh Cancer Research Laboratories, Chicago Medical School, Rosalind Franklin University of Medicine and Science, North Chicago, IL 60064 (United States)

    2010-11-26

    Research highlights: {yields} STAT5 interacts with a mitochondrial protein PDC-E2 in a leukemic T cell line LSTRA. {yields} Tyrosine-phosphorylated STAT5, but not STAT3, is present in LSTRA mitochondria. {yields} Cytokines induce mitochondrial translocation of STAT5, but not STAT1 or STAT3. {yields} Cytokine-induced mitochondrial translocation of tyrosine-phosphorylated STAT5 is transient. {yields} Mitochondrial STAT5 binds to a putative STAT5 site in the mitochondrial DNA in vitro. -- Abstract: Signal transducers and activators of transcription (STATs) were first identified as key signaling molecules in response to cytokines. Constitutive STAT activation also has been widely implicated in oncogenesis. We analyzed STAT5-associated proteins in a leukemic T cell line LSTRA, which exhibits constitutive tyrosine phosphorylation and activation of STAT5. A cellular protein was found to specifically interact with STAT5 in LSTRA cells by co-immunoprecipitation. Sequencing analysis and subsequent immunoblotting confirmed the identity of this STAT5-associated protein as the E2 component of mitochondrial pyruvate dehydrogenase complex (PDC-E2). Consistent with this interaction, both subcellular fractionation and immunofluorescence microscopy revealed mitochondrial localization of STAT5 in LSTRA cells. Mitochondrial localization of tyrosine-phosphorylated STAT5 also occurred in cytokine-stimulated cells. A time course experiment further demonstrated the transient kinetics of STAT5 mitochondrial translocation after cytokine stimulation. In contrast, cytokine-induced STAT1 and STAT3 activation did not result in their translocation into mitochondria. Furthermore, we showed that mitochondrial STAT5 bound to the D-loop regulatory region of mitochondrial DNA in vitro. It suggests a potential role of STAT5 in regulating the mitochondrial genome. Proliferative metabolism toward aerobic glycolysis is well known in cancer cells as the Warburg effect and is also observed in cytokine

  19. Pressure Transducer Locations

    Data.gov (United States)

    National Aeronautics and Space Administration — Files are located here, defining the locations of the pressure transducers on the HIRENASD model. These locations also correspond to the locations that analysts...

  20. Suppression of human lung cancer cell proliferation and metastasis in vitro by the transducer of ErbB-2.1(TOB1)

    Institute of Scientific and Technical Information of China (English)

    Yang JIAO; Ke-kang SUN; Lin ZHAO; Jia-ying XU; Li-li Wang; Sai-jun FAN

    2012-01-01

    Aim:To investigate the effects of the transducer of ErbB-2.1 (TOB1) on the proliferation,migration and invasion of human lung cancer cells in vitro.Methods:Human lung cancer cell lines (95-D,A549,NCI-H1299,NCI-H1975,NCI-H661,NCI-H446,NCI-H1395,and Calu-3)and the normal human bronchial epithelial (HBE) cell line were tested.The expression levels of TOB1 in the cells were determined with Western blot and RT-PCR analyses.TOB1-overexpressing cell line 95-D/TOB1 was constructed using lipofectamine-induced TOB1 recombinant plasmid transfection and selective G418 cell culture.The A549 cells were transcend-transfected with TOB1-siRNA.MTT assay,flow cytometry and Western blot analysis were used to examine the effects of TOB1 on cancer cell proliferation and wound healing.Transwell invasive assay was performed to evaluate the effects of TOB1 on cancer cell migration and invasion.The activity of MMP2 and MMP9 was measured using gelatin zymography assay.Results:The expression levels of TOB1 in the 8 human lung cancer cell lines were significantly lower than that in HBE cells.TOB1 overexpression inhibited the proliferation of 95-D cells,whereas TOB1 knockdown with TOB1-siRNA promoted the growth of A549 cells.Decreased cell migration and invasion were detected in 95-D/TOB1 cells,and the suppression of TOB1 enhanced the metastasis in A549 cells.TOB1 overexpression not only increased the expression of the phosphatase and tensin homolog (PTEN),an important tumor suppressor,but also regulated the downstream effectors in the PI3K/PTEN signaling pathway,including Akt,ERK1/2,etc.In contrast,decreased expression of TOB1 oppositely regulated the expression of these factors.TOB1 also regulates the gelatinase activity of MMP2 and MMP9 in lung cancer cells.Conclusion:The results demonstrate that the PI3K/PTEN pathway,which is essential for carcinogenesis,angiogenesis,and metastasis,may be one of the possible signaling pathways for regulation of proliferation and metastasis of human lung

  1. Dietary agent, benzyl isothiocyanate inhibits signal transducer and activator of transcription 3 phosphorylation and collaborates with sulforaphane in the growth suppression of PANC-1 cancer cells

    Directory of Open Access Journals (Sweden)

    Deangelis Stephanie

    2009-08-01

    Full Text Available Abstract The Signal Transducer and Activator of Transcription (STAT proteins comprise a family of latent transcription factors with diverse functions. STAT3 has well established roles in cell proliferation, growth and survival, and its persistent activation has been detected with high frequency in many human cancers. As constitutive activation of STAT3 appears to be vital for the continued survival of these cancerous cells, it has emerged as an attractive target for chemotherapeutics. We examined whether the inhibitory activities of bioactive compounds from cruciferous vegetables, such as Benzyl isothiocyanate (BITC and sulforaphane, extended to STAT3 activation in PANC-1 human pancreatic cancer cells. BITC and sulforaphane were both capable of inhibiting cell viability and inducing apoptosis in PANC-1. Sulforaphane had minimal effect on the direct inhibition of STAT3 tyrosine phosphorylation, however, suggesting its inhibitory activities are most likely STAT3-independent. Conversely, BITC was shown to inhibit the tyrosine phosphorylation of STAT3, but not the phosphorylation of ERK1/2, MAPK and p70S6 kinase. These results suggest that STAT3 may be one of the targets of BITC-mediated inhibition of cell viability in PANC-1 cancer cells. In addition, we show that BITC can prevent the induction of STAT3 activation by Interleukin-6 in MDA-MB-453 breast cancer cells. Furthermore, combinations of BITC and sulforaphane inhibited cell viability and STAT3 phosphorylation more dramatically than either agent alone. These findings suggest that the combination of the dietary agents BITC and sulforaphane has potent inhibitory activity in pancreatic cancer cells and that they may have translational potential as chemopreventative or therapeutic agents.

  2. Signal Transducer and Activator of Transcription 1 (STAT1) is Essential for Chromium Silencing of Gene Induction in Human Airway Epithelial Cells

    Science.gov (United States)

    Nemec, Antonia A.; Barchowsky, Aaron

    2009-01-01

    Hexavalent chromium (Cr(VI)) promotes lung injury and pulmonary diseases through poorly defined mechanisms that may involve the silencing of inducible protective genes. The current study investigated the hypothesis that Cr(VI) actively signals through a signal transducer and activator of transcription 1 (STAT1)–dependent pathway to silence nickel (Ni)–induced expression of vascular endothelial cell growth factor A (VEGFA), an important mediator of lung injury and repair. In human bronchial airway epithelial (BEAS-2B) cells, Ni-induced VEGFA transcription by stimulating an extracellular regulated kinase (ERK) signaling cascade that involved Src kinase–activated Sp1 transactivation, as well as increased hypoxia-inducible factor-1α (HIF-1α) stabilization and DNA binding. Ni-stimulated ERK, Src, and HIF-1α activities, as well as Ni-induced VEGFA transcript levels were inhibited in Cr(VI)-exposed cells. We previously demonstrated that Cr(VI) stimulates STAT1 to suppress VEGFA expression. In BEAS-2B cells stably expressing STAT1 short hairpin RNA, Cr(VI) increased VEGFA transcript levels and Sp1 transactivation. Moreover, in the absence of STAT1, Cr(VI), and Ni coexposures positively interacted to further increase VEGFA transcripts. This study demonstrates that metal-stimulated signaling cascades interact to regulate transcription and induction of adaptive or repair responses in airway cells. In addition, the data implicate STAT1 as a rate limiting mediator of Cr(VI)-stimulated gene regulation and suggest that cells lacking STAT1, such as many tumor cell lines, have opposite responses to Cr(VI) relative to normal cells. PMID:19403854

  3. Combined immunotherapy with granulocyte-macrophage colony-stimulating factor-transduced allogeneic prostate cancer cells and ipilimumab in patients with metastatic castration-resistant prostate cancer: a phase 1 dose-escalation trial

    NARCIS (Netherlands)

    Eertwegh, A.J. van den; Versluis, J.; Berg, H.P. van den; Santegoets, S.J.; Moorselaar, R.J. van; Sluis, T.M. van der; Gall, H.E.; Harding, T.C.; Jooss, K.; Lowy, I.; Pinedo, H.M.; Scheper, R.J.; Stam, A.G.; Blomberg, B.M. von; Gruijl, T.D. de; Hege, K.; Sacks, N.; Gerritsen, W.R.

    2012-01-01

    BACKGROUND: The granulocyte-macrophage colony-stimulating factor-transduced allogeneic prostate cancer cells vaccine (GVAX) has antitumour activity against prostate cancer; preclinical studies have shown potent synergy when combined with ipilimumab, an antibody that blocks cytotoxic T-lymphocyte ant

  4. Circular PVDF Airborne Transducer

    Institute of Scientific and Technical Information of China (English)

    JIAO Li-hua; XU Li-mei; HONG Hu

    2007-01-01

    With the required increased audio pressure of the parametric ultrasonic transducer array and the difficulty to theoretically analyse the complex ultrasonic structure in audio beam application, an computafionally efficient model is desired to describe the characteristic of the parametric ultrasonic transducer array for the system design and optimization. By applying the symmetry boundary conditions at the mid-plane in the thickness direction, a finite element model based on the half thickness simplification is presented to analyze the parametric circular transducer which is designed by gluing the poly Vinylidene fluoride film (PVDF). The validity of the proposed model is confirmed by a comparison of finite element aalysis results with the theoretical value and experimental data, which show that they are making a good agreement with each other.

  5. Volume measurements of the cell destruction zone and thermal decomposition zone in the focus of a shock wave transducer

    Science.gov (United States)

    Mastikhin, Igor; Teslenko, Vyacheslav; Nikolin, Valery

    2001-05-01

    Evaluation of the volume of the cell destruction zone is of interest in biomedical applications of shock waves (SW). The volume depends on mechanical properties of the cell membranes and is different for different cell types. In this work, we evaluated the cell destruction volume for two different cell types, tumor cells Crebs-2 and red blood cells. We used 0 70 0.5-s SW pulses with 45-MPa pressure in the focal zone. The concentration of destroyed cells was counted by dyeing in the case of tumor cells, and by spectrometry of released hemoglobin in the case of RBC. The cell destruction volume was calculated from destruction versus pulse number data and measured as 0.0135 ml for tumor cells. For RBC, the volume was 0.021 ml. To evaluate the effective volume of thermal zone, we used EPR signal of stable disulphide biradicals. Under SW action, S-S bonds of the biradicals rupture. The volume measurements were 0.003 ml. Since for that biradical, S-S bonds rupture at temperatures >80°C, and concentration of free radicals was an order lower (measured by spin traps) than of the produced monoradical, the rupture was caused by thermal decomposition. Thermal effects can play a significant role in SW action.

  6. Konstruktion af transducer

    DEFF Research Database (Denmark)

    Henriksen, Lars; Nielsen, Martin Pram

    Formålet med dette midtvejsprojekt er at udarbejde en transducer til måling af pressers stivhed. Dette er gjort på baggrund af en gennemgang af både presse- og stativ-typer samtidig med at udbøjningssituationen beskrives. Der introduceres en ide, der udgør grundkonceptet for opmålingsproceduren o...... færdige transducer – Load cellen. Strain gauge sørger for dataopsamlingen fra load cellen. Disse kalibreres således at transduceren er klar til de videre målinger der ligger i forlængelse af dette projekt....

  7. Human tumor-derived genomic DNA transduced into a recipient cell induces tumor-specific immune responses ex vivo

    OpenAIRE

    Whiteside, Theresa L.; Gambotto, Andrea; Albers, Andreas; Stanson, Joanna; Cohen, Edward P.

    2002-01-01

    This article describes a DNA-based vaccination strategy evaluated ex vivo with human cells. The vaccine was prepared by transferring tumor-derived genomic DNA to PCI-13 cells, a highly immunogenic tumor cell line (“recipient cell”), which had been genetically modified to secrete IL-2 (PCI-13/IL-2). PCI-13 cells expressed class I MHC determinants (HLA-A2) shared with the tumor from which the DNA was obtained as well as allogeneic determinants. DNA from a gp100+ melanoma ce...

  8. Quercetin induces caspase-dependent extrinsic apoptosis through inhibition of signal transducer and activator of transcription 3 signaling in HER2-overexpressing BT-474 breast cancer cells.

    Science.gov (United States)

    Seo, Hye-Sook; Ku, Jin Mo; Choi, Han-Seok; Choi, Youn Kyung; Woo, Jong-Kyu; Kim, Minsoo; Kim, Ilhwan; Na, Chang Hyeok; Hur, Hansol; Jang, Bo-Hyoung; Shin, Yong Cheol; Ko, Seong-Gyu

    2016-07-01

    Flavonoids are assumed to exert beneficial effects in different types of cancers at high concentrations. Yet, their molecular mechanisms of action remain unknown. The present study aimed to examine the effect of quercetin on proliferation and apoptosis in HER2-expressing breast cancer cells. The anti-proliferative effects of quercetin were examined by proliferation, MTT and clonogenic survival assays. The effect of quercetin on expression of apoptotic molecules was determined by western blotting. Luciferase reporter assay was performed to measure signal transducer and activator of transcription 3 (STAT3) transcriptional activity. ELISA assay was performed to measure intracellular MMP-9 levels. Immunocytochemistry was performed to evaluate the nuclear STAT3 level. The results revealed that quercetin inhibited the proliferation of BT-474 cells in a dose- and time-dependent manner. Quercetin also inhibited clonogenic survival (anchorage-dependent and -independent) of BT-474 cells in a dose-dependent manner. These growth inhibitions were accompanied with an increase in sub-G0/G1 apoptotic populations. Quercetin induced caspase-dependent extrinsic apoptosis upregulating the levels of cleaved caspase-8 and cleaved caspase-3, and inducing the cleavage of poly(ADP‑ribose) polymerase (PARP). In contrast, quercetin did not induce apoptosis via intrinsic mitochondrial apoptosis pathway since this compound did not decrease the mitochondrial membrane potential and did not affect the levels of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (BAX). Quercetin reduced the expression of phospho-JAK1 and phospho-STAT3 and decreased STAT3-dependent luciferase reporter gene activity in the BT-474 cells. Quercetin inhibited MMP-9 secretion and decreased the nuclear translocation of STAT3. Our study indicates that quercetin induces apoptosis at concentrations >20 µM through inhibition of STAT3 signaling and could serve as a useful compound to prevent or treat HER2

  9. Signal transducer and activator of transcription 3 and 5 regulate system Xc- and redox balance in human breast cancer cells.

    Science.gov (United States)

    Linher-Melville, Katja; Haftchenary, Sina; Gunning, Patrick; Singh, Gurmit

    2015-07-01

    System Xc- is a cystine/glutamate antiporter that contributes to the maintenance of cellular redox balance. The human xCT (SLC7A11) gene encodes the functional subunit of system Xc-. Transcription factors regulating antioxidant defense mechanisms including system Xc- are of therapeutic interest, especially given that aggressive breast cancer cells exhibit increased system Xc- function. This investigation provides evidence that xCT expression is regulated by STAT3 and/or STAT5A, functionally affecting the antiporter in human breast cancer cells. Computationally analyzing two kilobase pairs of the xCT promoter/5' flanking region identified a distal gamma-activated site (GAS) motif, with truncations significantly increasing luciferase reporter activity. Similar transcriptional increases were obtained after treating cells transiently transfected with the full-length xCT promoter construct with STAT3/5 pharmacological inhibitors. Knock-down of STAT3 or STAT5A with siRNAs produced similar results. However, GAS site mutation significantly reduced xCT transcriptional activity, suggesting that STATs may interact with other transcription factors at more proximal promoter sites. STAT3 and STAT5A were bound to the xCT promoter in MDA-MB-231 cells, and binding was disrupted by pre-treatment with STAT inhibitors. Pharmacologically suppressing STAT3/5 activation significantly increased xCT mRNA and protein levels, as well as cystine uptake, glutamate release, and total levels of intracellular glutathione. Our data suggest that STAT proteins negatively regulate basal xCT expression. Blocking STAT3/5-mediated signaling induces an adaptive, compensatory mechanism to protect breast cancer cells from stress, including reactive oxygen species, by up-regulating xCT expression and the function of system Xc-. We propose that targeting system Xc- together with STAT3/5 inhibitors may heighten therapeutic anti-cancer effects.

  10. Nano-optomechanical transducer

    Science.gov (United States)

    Rakich, Peter T; El-Kady, Ihab F; Olsson, Roy H; Su, Mehmet Fatih; Reinke, Charles; Camacho, Ryan; Wang, Zheng; Davids, Paul

    2013-12-03

    A nano-optomechanical transducer provides ultrabroadband coherent optomechanical transduction based on Mach-wave emission that uses enhanced photon-phonon coupling efficiencies by low impedance effective phononic medium, both electrostriction and radiation pressure to boost and tailor optomechanical forces, and highly dispersive electromagnetic modes that amplify both electrostriction and radiation pressure. The optomechanical transducer provides a large operating bandwidth and high efficiency while simultaneously having a small size and minimal power consumption, enabling a host of transformative phonon and signal processing capabilities. These capabilities include optomechanical transduction via pulsed phonon emission and up-conversion, broadband stimulated phonon emission and amplification, picosecond pulsed phonon lasers, broadband phononic modulators, and ultrahigh bandwidth true time delay and signal processing technologies.

  11. Stress wave focusing transducers

    Energy Technology Data Exchange (ETDEWEB)

    Visuri, S.R., LLNL

    1998-05-15

    Conversion of laser radiation to mechanical energy is the fundamental process behind many medical laser procedures, particularly those involving tissue destruction and removal. Stress waves can be generated with laser radiation in several ways: creation of a plasma and subsequent launch of a shock wave, thermoelastic expansion of the target tissue, vapor bubble collapse, and ablation recoil. Thermoelastic generation of stress waves generally requires short laser pulse durations and high energy density. Thermoelastic stress waves can be formed when the laser pulse duration is shorter than the acoustic transit time of the material: {tau}{sub c} = d/c{sub s} where d = absorption depth or spot diameter, whichever is smaller, and c{sub s} = sound speed in the material. The stress wave due to thermoelastic expansion travels at the sound speed (approximately 1500 m/s in tissue) and leaves the site of irradiation well before subsequent thermal events can be initiated. These stress waves, often evolving into shock waves, can be used to disrupt tissue. Shock waves are used in ophthalmology to perform intraocular microsurgery and photodisruptive procedures as well as in lithotripsy to fragment stones. We have explored a variety of transducers that can efficiently convert optical to mechanical energy. One such class of transducers allows a shock wave to be focused within a material such that the stress magnitude can be greatly increased compared to conventional geometries. Some transducer tips could be made to operate regardless of the absorption properties of the ambient media. The size and nature of the devices enable easy delivery, potentially minimally-invasive procedures, and precise tissue- targeting while limiting thermal loading. The transducer tips may have applications in lithotripsy, ophthalmology, drug delivery, and cardiology.

  12. Numerical transducer modelling

    DEFF Research Database (Denmark)

    Cutanda, Vicente

    1999-01-01

    Numerical modelling is of importance for the design, improvement and study of acoustic transducers such as microphones and accelerometers. Techniques like the boundary element method and the finite element method are the most common supplement to the traditional empirical and analytical approaches...... errors and instabilities in the computations of numerical solutions. An investigation to deal with this narrow-gap problem has been carried out....

  13. A Force Transducer from a Junk Electronic Balance

    Science.gov (United States)

    Aguilar, Horacio Munguia; Aguilar, Francisco Armenta

    2009-01-01

    It is shown how the load cell from a junk electronic balance can be used as a force transducer for physics experiments. Recovering this device is not only an inexpensive way of getting a valuable laboratory tool but also very useful didactic work on electronic instrumentation. Some experiments on mechanics with this transducer are possible after a…

  14. Transcriptional Regulatory Networks Activated by PI3K and ERK Transduced Growth Signals in Human Glioblastoma Cells

    Institute of Scientific and Technical Information of China (English)

    Peter M. Haverty; Zhi-Ping Weng; Ulla Hansen

    2005-01-01

    Determining how cells regulate their transcriptional response to extracellular signals is key to the understanding of complex eukaryotic systems. This study was initiated with the goals of furthering the study of mammalian transcriptional regulation and analyzing the relative benefits of related computational methodologies. One dataset available for such an analysis involved gene expression profiling of the early growth factor response to platelet derived growth factor (PDGF)in a human glioblastoma cell line; this study differentiated genes whose expression was regulated by signaling through the phosphoinositide-3-kinase (PI3K) versus the extracellular-signal regulated kinase (ERK) pathways. We have compared the inferred transcription factors from this previous study with additional predictions of regulatory transcription factors using two alternative promoter sequence analysis techniques. This comparative analysis, in which the algorithms predict overlapping,although not identical, sets of factors, argues for meticulous benchmarking of promoter sequence analysis methods to determine the positive and negative attributes that contribute to their varying results. Finally, we inferred transcriptional regulatory networks deriving from various signaling pathways using the CARRIE program suite. These networks not only included previously described transcriptional features of the response to growth signals, but also predicted new regulatory features for the propagation and modulation of the growth signal.

  15. Specific micro RNA-regulated TetR-KRAB transcriptional control of transgene expression in viral vector-transduced cells.

    Directory of Open Access Journals (Sweden)

    Virginie Pichard

    Full Text Available Precise control of transgene expression in a tissue-specific and temporally regulated manner is desirable for many basic and applied investigations gene therapy applications. This is important to regulate dose of transgene products and minimize unwanted effects. Previously described methods have employed tissue specific promoters, miRNA-based transgene silencing or tetR-KRAB-mediated suppression of transgene promoters. To improve on versatility of transgene expression control, we have developed expression systems that use combinations of a tetR-KRAB artificial transgene-repressor, endogenous miRNA silencing machinery and tissue specific promoters. Precise control of transgene expression was demonstrated in liver-, macrophage- and muscle-derived cells. Efficiency was also demonstrated in vivo in murine muscle. This multicomponent and modular regulatory system provides a robust and easily adaptable method for achieving regulated transgene expression in different tissue types. The improved precision of regulation will be useful for many gene therapy applications requiring specific spatiotemporal transgene regulation.

  16. Transducers for ultrasonic limb plethysmography

    Science.gov (United States)

    Nickell, W. T.; Wu, V. C.; Bhagat, P. K.

    1983-01-01

    The design, construction, and performance characteristics of ultasonic transducers suitable for limb plethysmography are presented. Both 3-mm-diameter flat-plate and 12-mm-diameter hemispheric ceramic transducers operating at 2 MHz were fitted in 1-mm thick epoxy-resin lens/acoustic-coupling structures and mounted in exercie-EKG electrode housings for placement on the calf using adhesive collars. The effects of transducer directional characteristics on performance under off-axis rotation and the electrical impedances of the transducers were measured: The flat transducer was found to be sensitive to rotation and have an impedance of 800 ohms; the hemispheric transducer, to be unaffected by rotation and have an impedance of 80 ohms. The use of hemispheric transducers as both transmitter and receiver, or of a flat transducer as transmitter and a hemispheric transducer as receiver, was found to produce adequate dimensional measurements, with minimum care in transducer placement, in short-term physiological experiments and long-term (up to 7-day) attachment tests.

  17. Helicobacter pylori inhibits dendritic cell maturation via interleukin-10-mediated activation of the signal transducer and activator of transcription 3 pathway.

    Science.gov (United States)

    Rizzuti, David; Ang, Michelle; Sokollik, Christiane; Wu, Ted; Abdullah, Majd; Greenfield, Laura; Fattouh, Ramzi; Reardon, Colin; Tang, Michael; Diao, Jun; Schindler, Christian; Cattral, Mark; Jones, Nicola L

    2015-01-01

    Helicobacter pylori infects the human gastric mucosa causing a chronic infection that is the primary risk factor for gastric cancer development. Recent studies demonstrate that H. pylori promotes tolerogenic dendritic cell (DC) development indicating that this bacterium evades the host immune response. However, the signaling pathways involved in modulating DC activation during infection remain unclear. Here, we report that H. pylori infection activated the signal transducer and activator of transcription 3 (STAT3) pathway in murine bone marrow-derived DCs (BMDCs) and splenic DCs isolated ex vivo. Isogenic cagA-, cagE-, vacA- and urease-mutants exhibited levels of phosphoSTAT3 that were comparable to in the wild-type (WT) parent strain. H. pylori-infected BMDCs produced increased immunosuppressive IL-10, which activated STAT3 in an autocrine/paracrine fashion. Neutralization of IL-10 prevented H. pylori-mediated STAT3 activation in both BMDCs and splenic DCs. In addition, anti-IL-10 treatment of infected H. pylori-BMDCs was associated with increased CD86 and MHC II expression and enhanced proinflammatory IL-1β cytokine secretion. Finally, increased CD86 and MHC II expression was detected in H. pylori-infected STAT3 knockout DCs when compared to WT controls. Together, these results demonstrate that H. pylori infection induces IL-10 secretion in DCs, which activates STAT3, thereby modulating DC maturation and reducing IL-1β secretion. These findings identify a host molecular mechanism by which H. pylori can manipulate the innate immune response to potentially favor chronic infection and promote carcinogenesis. PMID:25412627

  18. Infection-stimulated Anemia Results Primarily from Interferon Gamma-dependent, Signal Transducer and Activator of Transcription 1-independent Red Cell Loss

    Institute of Scientific and Technical Information of China (English)

    Zheng Wang; Dong-Xia Zhang; Qi Zhao

    2015-01-01

    Background:Although the onset of anemia during infectious disease is commonly correlated with production of inflammatory cytokines,the mechanisms by which cytokines induce anemia are poorly defined.This study focused on the mechanism research.Methods:Different types of mice were infected perorally with Toxoplasma gondii strain ME49.At the indicated times,samples from each mouse were harvested,processed,and analyzed individually.Blood samples were analyzed using a Coulter Counter and red blood cell (RBC) survival was measured by biotinylation.Levels of tumor necrosis factor-α (TNF-α),inducible nitric oxide synthase (iNOS),and inducible protein 10 (IP-10) mRNA in liver tissue were measured by real-time polymerase chain reaction.Results:T.gondii-infected mice exhibited anemia due to a decrease in both erythropoiesis and survival time of RBC in the circulation (P < 0.02).In addition,infection-stimulated anemia was associated with fecal occult,supporting previous literature that hemorrhage is a consequence of T.gondii infection in mice.Infection-induced anemia was abolished in interferon gamma (IFNγ) and IFNγ receptor deficient mice (P < 0.05) but was still evident in mice lacking TNF-α,iNOS,phagocyte NADPH oxidase or IP-10 (P < 0.02).Neither signal transducer and activator of transcription 1 (STAT1) deficient mice nor 129S6 controls exhibited decreased erythropoiesis,but rather suffered from an anemia resulting solely from increased loss of circulating RBC.Conclusions:Infection-stimulated decrease in erythropoiesis and losses of RBC have distinct mechanistic bases.These results show that during T.gondii infection,IFNγ is responsible for an anemia that results from both a decrease in erythropoiesis and a STAT1 independent loss of circulating RBC.

  19. Hair cell ribbon synapses

    OpenAIRE

    Moser, Tobias; Brandt, Andreas; Lysakowski, Anna

    2006-01-01

    Hearing and balance rely on the faithful synaptic coding of mechanical input by the auditory and vestibular hair cells of the inner ear. Mechanical deflection of their stereocilia causes the opening of mechanosensitive channels, resulting in hair cell depolarization, which controls the release of glutamate at ribbon-type synapses. Hair cells have a compact shape with strong polarity. Mechanoelectrical transduction and active membrane turnover associated with stereociliar renewal dominate the ...

  20. Developmental evolutionary biology of the vertebrate ear: conserving mechanoelectric transduction and developmental pathways in diverging morphologies

    Science.gov (United States)

    Fritzsch, B.; Beisel, K. W.; Bermingham, N. A.

    2000-01-01

    This brief overview shows that a start has been made to molecularly dissect vertebrate ear development and its evolutionary conservation to the development of the insect hearing organ. However, neither the patterning process of the ear nor the patterning process of insect sensory organs is sufficiently known at the moment to provide more than a first glimpse. Moreover, hardly anything is known about otocyst development of the cephalopod molluscs, another triploblast lineage that evolved complex 'ears'. We hope that the apparent conserved functional and cellular components present in the ciliated sensory neurons/hair cells will also be found in the genes required for vertebrate ear and insect sensory organ morphogenesis (Fig. 3). Likewise, we expect that homologous pre-patterning genes will soon be identified for the non-sensory cell development, which is more than a blocking of neuronal development through the Delta/Notch signaling system. Generation of the apparently unique ear could thus represent a multiplication of non-sensory cells by asymmetric and symmetric divisions as well as modification of existing patterning process by implementing novel developmental modules. In the final analysis, the vertebrate ear may come about by increasing the level of gene interactions in an already existing and highly conserved interactive cascade of bHLH genes. Since this was apparently achieved in all three lineages of triploblasts independently (Fig. 3), we now need to understand how much of the morphogenetic cascades are equally conserved across phyla to generate complex ears. The existing mutations in humans and mice may be able to point the direction of future research to understand the development of specific cell types and morphologies in the formation of complex arthropod, cephalopod, and vertebrate 'ears'.

  1. Intravitreal Injection of AAV2 Transduces Macaque Inner Retina

    OpenAIRE

    Yin, Lu; Greenberg, Kenneth; Hunter, Jennifer J.; Dalkara, Deniz; Kolstad, Kathleen D; Masella, Benjamin D.; Wolfe, Robert; Visel, Meike; Stone, Daniel; Libby, Richard T.; DiLoreto, David; Schaffer, David; Flannery, John; Williams, David R.; Merigan, William H.

    2011-01-01

    Intravitreally injected AAV2 transduced inner retinal cells in a restricted region at the macaque fovea. Because macaque and human eyes are similar, the results suggest a need to improve transduction methods in gene therapy for the human inner retina.

  2. Optically transduced MEMS magnetometer

    Science.gov (United States)

    Nielson, Gregory N; Langlois, Eric

    2014-03-18

    MEMS magnetometers with optically transduced resonator displacement are described herein. Improved sensitivity, crosstalk reduction, and extended dynamic range may be achieved with devices including a deflectable resonator suspended from the support, a first grating extending from the support and disposed over the resonator, a pair of drive electrodes to drive an alternating current through the resonator, and a second grating in the resonator overlapping the first grating to form a multi-layer grating having apertures that vary dimensionally in response to deflection occurring as the resonator mechanically resonates in a plane parallel to the first grating in the presence of a magnetic field as a function of the Lorentz force resulting from the alternating current. A plurality of such multi-layer gratings may be disposed across a length of the resonator to provide greater dynamic range and/or accommodate fabrication tolerances.

  3. Numerical Transducer Modeling

    DEFF Research Database (Denmark)

    Henriquez, Vicente Cutanda

    This thesis describes the development of a numerical model of the propagation of sound waves in fluids with viscous and thermal losses, with application to the simulation of acoustic transducers, in particular condenser microphones for measurement. The theoretical basis is presented, numerical...... that are allowable in this case: linear variations, absence of flow, harmonic time variation, thermodynamical equilibrium and physical dimensions much larger than the molecular mean free path. A formulation of the BEM is also developed with an improvement designed to cope with the numerical difficulty associated...... with very close surfaces, as found in condenser microphones, where the membrane has a backplate very close behind. This improvement could be useful for many other problems where the BEM is applied. The numerical implementation that includes both viscous and thermal effects is then worked out. Some numerical...

  4. Calibration of Underwater Sound Transducers

    Directory of Open Access Journals (Sweden)

    H.R.S. Sastry

    1983-07-01

    Full Text Available The techniques of calibration of underwater sound transducers for farfield, near-field and closed environment conditions are reviewed in this paper .The design of acoustic calibration tank is mentioned. The facilities available at Naval Physical & Oceanographic Laboratory, Cochin for calibration of transducers are also listed.

  5. Longitudinal immune monitoring of patients receiving intratumoral injection of a MART-1 T-cell receptor-transduced cell line (C-Cure 709)

    DEFF Research Database (Denmark)

    Køllgaard, Tania; Duval, Lone; Schmidt, Henrik;

    2009-01-01

    BACKGROUND AIMS: Adoptive transfer of tumor-specific lymphocytes is a promising strategy in the treatment of cancer. We conducted intratumoral administration of an allogeneic irradiated continuous T-cell line (C-Cure 709) expressing an HLA-A2-restricted MART-1-specific T-cell receptor (TCR......) into HLA-A2(+) melanoma patients. The C-Cure 709 cell line is cytotoxic against MART-1(+) HLA-A2(+) melanoma cell lines and secretes several immune stimulatory cytokines upon stimulation. METHODS: Anti-tumor immune responses against the commonly expressed tumor antigen (Ag) MART-1 were longitudinally...... analyzed in peripheral blood by fluorescence-activated cell sorting (FACS) before and after intratumoral injection of C-Cure 709. RESULTS: No treatment-induced increase in Ag-specific T-cell frequencies was observed in peripheral blood, and the phenotype of MART-1-specific T cells was very stable during...

  6. Inhibition of the growth of human hepatoma cell line both in vitro and in vivo by transducing CKI gene p21WAF-1 with GE7 targeting gene delivery system

    Institute of Scientific and Technical Information of China (English)

    2000-01-01

    The EGF receptor-mediated targeting gene delivery system GE7 was used to transduce exogenous gene pCEP-p21WAF-1 into human hepatocellular carcinoma cell both in vitro and in vivo. After in vitro transduction of the exogenous gene, the growth of the cell lines SMMC-7721 and BEL-7402 was significantly inhibited compared with the control. On day 8 the inhibition rates of the above cell lines reached 56.0% and 66.7%, respectively. The in vivo experiment showed that the growth of human hepatoma transplanted in nude mice injected with GE7 gene delivery system subcutaneously once a week for 3 weeks was remarkably inhibited compared with that of untransfected control. The average tumor weight of the experiment group was (0.083 ± 0.043) g, while that of the control group was (0.281± 0.173) g. The difference is significant (P<0.05). It was indicated that GE7 gene delivery system could efficiently transduce exogenous gene pCEP-p21WAF-1 into hepatoma cell with high EGF receptor expression, and inhibit the cell growth with high efficacy both in vivo and in vitro.

  7. Circuit for Driving Piezoelectric Transducers

    Science.gov (United States)

    Randall, David P.; Chapsky, Jacob

    2009-01-01

    The figure schematically depicts an oscillator circuit for driving a piezoelectric transducer to excite vibrations in a mechanical structure. The circuit was designed and built to satisfy application-specific requirements to drive a selected one of 16 such transducers at a regulated amplitude and frequency chosen to optimize the amount of work performed by the transducer and to compensate for both (1) temporal variations of the resonance frequency and damping time of each transducer and (2) initially unknown differences among the resonance frequencies and damping times of different transducers. In other words, the circuit is designed to adjust itself to optimize the performance of whichever transducer is selected at any given time. The basic design concept may be adaptable to other applications that involve the use of piezoelectric transducers in ultrasonic cleaners and other apparatuses in which high-frequency mechanical drives are utilized. This circuit includes three resistor-capacitor networks that, together with the selected piezoelectric transducer, constitute a band-pass filter having a peak response at a frequency of about 2 kHz, which is approximately the resonance frequency of the piezoelectric transducers. Gain for generating oscillations is provided by a power hybrid operational amplifier (U1). A junction field-effect transistor (Q1) in combination with a resistor (R4) is used as a voltage-variable resistor to control the magnitude of the oscillation. The voltage-variable resistor is part of a feedback control loop: Part of the output of the oscillator is rectified and filtered for use as a slow negative feedback to the gate of Q1 to keep the output amplitude constant. The response of this control loop is much slower than 2 kHz and, therefore, does not introduce significant distortion of the oscillator output, which is a fairly clean sine wave. The positive AC feedback needed to sustain oscillations is derived from sampling the current through the

  8. A Direct Driver for Electrostatic Transducers

    DEFF Research Database (Denmark)

    Nielsen, Dennis; Knott, Arnold; Andersen, Michael A. E.

    2014-01-01

    Electrostatic transducers represent a very interesting alternative to the traditional inefficient electrodynamic transducers. In order to establish the full potential of these transducers, power amplifiers which fulfill the strict requirements imposed by such loads (high impedance, frequency...

  9. Intravenous delivery of HIV-based lentiviral vectors preferentially transduces F4/80+ and Ly-6C+ cells in spleen, important target cells in autoimmune arthritis

    NARCIS (Netherlands)

    Brand, B.T. van den; Vermeij, E.A.; Waterborg, C.E.; Arntz, O.J.; Kracht, M.; Bennink, M.B.; Berg, W.B. van den; Loo, F.A. van de

    2013-01-01

    Antigen presenting cells (APCs) play an important role in arthritis and APC specific gene therapeutic targeting will enable intracellular modulation of cell activity. Viral mediated overexpression is a potent approach to achieve adequate transgene expression levels and lentivirus (LV) is useful for

  10. Amperometric biosensors based on carbon composite transducers

    Science.gov (United States)

    Lu, Fang

    1998-12-01

    supply of oxygen of a Kel-F/carbon paste enzyme transducer, even for microsensor, is not depleted over a prolonged operation under oxygen-deficient conditions. The oxygen effect upon the kinetic parameters of the enzyme-based carbon paste is examined for a variety of pasting binders. Such attention to the oxygen dependence of enzyme-based transducers addresses numerous practical biosensing applications, involving the oxygen demand and micro-probe in monitoring of glucose. Novel biocomposites based on the incorporation of the tyrosinase into a graphite-epoxy resin and a metal-dispersed carbon paste matrix, were used for the effective biosensing of phenolic compounds. The enzyme retains its bioactivity on confinement in these carbon composite environments. Hence, convenient quantification of micromolar concentrations is feasible at the electrochemical reduction of phenolic compounds with a fast, sensitive and reproducible response compared to conventional tyrosinase-based biosensors. The influence of various experimental variables was explored for optimum biosensing performance in flow-injection monitoring of phenolic compounds. A dual-biosensor detector provides a suitable method for detecting phenolic compounds in environmental samples after HPLC separation. Amperometric detection using a dual-biosesnor thin-layer cell in the parallel configuration aided in the identification of unknown phenolic components in complicated environmental samples. As a result, detection of eluting phenolic compounds is improved with respect to further selectivity and stability in liquid chromatography. Selectivity is enhanced by obtaining two chromatograms of parallel opposed detectors. Stability is improved by using solid paraffin transducer composition that can be operated well under the conditions of high pressure and high content of organic solvent. This method has proven suitable for analysis of phenolic compounds in environmental samples.

  11. Frequency Steered Acoustic Transducer Project

    Data.gov (United States)

    National Aeronautics and Space Administration — This Small Business Innovation Research Phase I project is to develop, fabricate, and characterize a novel frequency steered acoustic transducer (FSAT) for the...

  12. Transducer Field Imaging Using Acoustography

    Directory of Open Access Journals (Sweden)

    Jaswinder S. Sandhu

    2012-01-01

    Full Text Available A common current practice for transducer field mapping is to scan, point-by-point, a hydrophone element in a 2D raster at various distances from the transducer radiating surface. This approach is tedious, requiring hours of scanning time to generate full cross-sectional and/or axial field distributions. Moreover, the lateral resolution of the field distribution image is dependent on the indexing steps between data points. Acoustography is an imaging process in which an acousto-optical (AO area sensor is employed to record the intensity of an ultrasound wavefield on a two-dimensional plane. This paper reports on the application of acoustography as a simple but practical method for assessing transducer field characteristics. A case study performed on a commercial transducer is reported, where the radiated fields are imaged using acoustography and compared to the corresponding quantities that are predicted numerically.

  13. Miniature, high efficiency transducers for use in ultrasonic flow meters

    Science.gov (United States)

    Saikia, Meghna

    This thesis is concerned with the development of a new type of miniature, high efficiency transducer for use in ultrasonic flow meters. The proposed transducer consists of a thin plate of a suitable piezoelectric material on which an inter-digital transducer is fabricated for the generation and detection of plate acoustic waves. When immersed in a fluid medium, this device can convert energy from plate acoustic waves (PAWs) into bulk acoustic waves (BAWs) and vice versa. It is shown that this mode coupling principle can be used to realize efficient transducers for use in ultrasonic flow meters. This transducer can be mounted flush with the walls of the pipe through which fluid is flowing, resulting in minimal disturbance of fluid flow. A prototype flow cell using these transducers has been designed and fabricated. The characteristics of this device have been measured over water flow rates varying from 0 to 7.5 liters per minute and found to be in good agreement with theory. Another attractive property of the new transducers is that they can be used to realize remotely read, passive, wireless flow meters. Details of methods that can be used to develop this wireless capability are described. The research carried out in this thesis has applications in several other areas such as ultrasonic nondestructive evaluation (NDE), noncontact or air coupled ultrasonics, and for developing wireless capability in a variety of other acoustic wave sensors.

  14. Identifying components of the hair-cell interactome involved in cochlear amplification

    Directory of Open Access Journals (Sweden)

    Cheatham MaryAnn

    2009-03-01

    Full Text Available Abstract Background Although outer hair cells (OHCs play a key role in cochlear amplification, it is not fully understood how they amplify sound signals by more than 100 fold. Two competing or possibly complementary mechanisms, stereocilia-based and somatic electromotility-based amplification, have been considered. Lacking knowledge about the exceptionally rich protein networks in the OHC plasma membrane, as well as related protein-protein interactions, limits our understanding of cochlear function. Therefore, we focused on finding protein partners for two important membrane proteins: Cadherin 23 (cdh23 and prestin. Cdh23 is one of the tip-link proteins involved in transducer function, a key component of mechanoelectrical transduction and stereocilia-based amplification. Prestin is a basolateral membrane protein responsible for OHC somatic electromotility. Results Using the membrane-based yeast two-hybrid system to screen a newly built cDNA library made predominantly from OHCs, we identified two completely different groups of potential protein partners using prestin and cdh23 as bait. These include both membrane bound and cytoplasmic proteins with 12 being de novo gene products with unknown function(s. In addition, some of these genes are closely associated with deafness loci, implying a potentially important role in hearing. The most abundant prey for prestin (38% is composed of a group of proteins involved in electron transport, which may play a role in OHC survival. The most abundant group of cdh23 prey (55% contains calcium-binding domains. Since calcium performs an important role in hair cell mechanoelectrical transduction and amplification, understanding the interactions between cdh23 and calcium-binding proteins should increase our knowledge of hair cell function at the molecular level. Conclusion The results of this study shed light on some protein networks in cochlear hair cells. Not only was a group of de novo genes closely associated

  15. Pressure Transducer Has Long Service Life

    Science.gov (United States)

    Prout, R. E.; Chaves, A. J.

    1982-01-01

    Differential-pressure transducer includes a piston, helical springs, and a linear variable-differential transformer concentric with piston. Transducer senses motion of piston in response to changes in pressure differential. Eight seals within the transducer prevent fluid leakage from one pressure line to the other. Reliability and operating life of the new unit are superior to many conventional transducers.

  16. Characterization of Dielectric Electroactive Polymer transducers

    DEFF Research Database (Denmark)

    Nielsen, Dennis; Møller, Martin B.; Sarban, Rahimullah;

    2014-01-01

    This paper analysis the small-signal model of the Dielectric Electro Active Polymer (DEAP) transducer. The DEAP transducer have been proposed as an alternative to the electrodynamic transducer in sound reproduction systems. In order to understand how the DEAP transducer works, and provide guideli...

  17. Artificial antigen-presenting cells transduced with telomerase efficiently expand epitope-specific, human leukocyte antigen-restricted cytotoxic T cells.

    Science.gov (United States)

    Dupont, Jakob; Latouche, Jean-Baptiste; Ma, Chia; Sadelain, Michel

    2005-06-15

    Human telomerase reverse transcriptase (hTERT) is overexpressed in most human tumors, making it a potential target for cancer immunotherapy. hTERT-derived CTL epitopes have been identified previously, including p865 (RLVDDFLLV) and p540 (ILAKFLHWL), which are restricted by the human leukocyte antigen (HLA) class I A*0201 allele. However, it remains a major challenge to efficiently and consistently expand hTERT-specific CTLs from donor peripheral blood T lymphocytes. To bypass the need for generating conventional antigen-presenting cells (APC) on an autologous basis, we investigated the potential ability of fibroblast-derived artificial APCs (AAPC) to activate and expand HLA-A*0201-restricted CTLs. We show here that AAPCs stably expressing HLA-A*0201, human beta(2)-microglobulin, B7.1, intercellular adhesion molecule-1, and LFA-3, together with either p540 and p865 minigenes or the full-length hTERT, effectively stimulate tumoricidal, hTERT-specific CTLs. hTERT-expressing AAPCs stimulated both p540 and p865 CTLs as shown by peptide-specific cytolysis and tetramer staining, indicating that hTERT is processed by the AAPCs and that the two peptides are presented as codominant epitopes. The level of cytotoxic activity against a panel of tumors comprising hematologic and epithelial malignancies varied, correlating overall with the level of HLA-A2 and hTERT expression by the target cell. Starting from 100 mL blood, approximately 100 million hTERT-specific CTLs could be generated over the course of five sequential stimulations, representing an expansion of approximately 1 x 10(5). Our data show that AAPCs process hTERT antigen and efficiently stimulate hTERT-specific CTLs from human peripheral blood T lymphocytes and suggest that sufficient expansion could be achieved to be clinically useful for adoptive cell therapy.

  18. Ultrasonic Transducer Irradiation Test Results

    International Nuclear Information System (INIS)

    Ultrasonic technologies offer the potential for high-accuracy and -resolution in-pile measurement of a range of parameters, including geometry changes, temperature, crack initiation and growth, gas pressure and composition, and microstructural changes. Many Department of Energy-Office of Nuclear Energy (DOE-NE) programs are exploring the use of ultrasonic technologies to provide enhanced sensors for in-pile instrumentation during irradiation testing. For example, the ability of small diameter ultrasonic thermometers (UTs) to provide a temperature profile in candidate metallic and oxide fuel would provide much needed data for validating new fuel performance models. Other ongoing efforts include an ultrasonic technique to detect morphology changes (such as crack initiation and growth) and acoustic techniques to evaluate fission gas composition and pressure. These efforts are limited by the lack of identified ultrasonic transducer materials capable of long term performance under irradiation test conditions. For this reason, the Pennsylvania State University (PSU) was awarded an ATR NSUF project to evaluate the performance of promising magnetostrictive and piezoelectric transducers in the Massachusetts Institute of Technology Research Reactor (MITR) up to a fast fluence of at least 1021 n/cm2. The goal of this research is to characterize and demonstrate magnetostrictive and piezoelectric transducer operation during irradiation, enabling the development of novel radiation-tolerant ultrasonic sensors for use in Material Testing Reactors (MTRs). As such, this test is an instrumented lead test and real-time transducer performance data is collected along with temperature and neutron and gamma flux data. The current work bridges the gap between proven out-of-pile ultrasonic techniques and in-pile deployment of ultrasonic sensors by acquiring the data necessary to demonstrate the performance of ultrasonic transducers. To date, one piezoelectric transducer and two

  19. Ultrasonic Transducer Irradiation Test Results

    Energy Technology Data Exchange (ETDEWEB)

    Daw, Joshua [Idaho National Lab. (INL), Idaho Falls, ID (United States); Palmer, Joe [Idaho National Lab. (INL), Idaho Falls, ID (United States); Ramuhalli, Pradeep [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Keller, Paul [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Montgomery, Robert [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Chien, Hual-Te [Argonne National Lab. (ANL), Argonne, IL (United States); Kohse, Gordon [MIT (Massachusetts Inst. of Technology), Cambridge, MA (United States); Tittmann, Bernhard [Pennsylvania State Univ., University Park, PA (United States); Reinhardt, Brian [Pennsylvania State Univ., University Park, PA (United States); Rempe, Joy [Rempe and Associates, Idaho Falls, ID (United States)

    2015-02-01

    Ultrasonic technologies offer the potential for high-accuracy and -resolution in-pile measurement of a range of parameters, including geometry changes, temperature, crack initiation and growth, gas pressure and composition, and microstructural changes. Many Department of Energy-Office of Nuclear Energy (DOE-NE) programs are exploring the use of ultrasonic technologies to provide enhanced sensors for in-pile instrumentation during irradiation testing. For example, the ability of small diameter ultrasonic thermometers (UTs) to provide a temperature profile in candidate metallic and oxide fuel would provide much needed data for validating new fuel performance models. Other ongoing efforts include an ultrasonic technique to detect morphology changes (such as crack initiation and growth) and acoustic techniques to evaluate fission gas composition and pressure. These efforts are limited by the lack of identified ultrasonic transducer materials capable of long term performance under irradiation test conditions. For this reason, the Pennsylvania State University (PSU) was awarded an ATR NSUF project to evaluate the performance of promising magnetostrictive and piezoelectric transducers in the Massachusetts Institute of Technology Research Reactor (MITR) up to a fast fluence of at least 1021 n/cm2. The goal of this research is to characterize and demonstrate magnetostrictive and piezoelectric transducer operation during irradiation, enabling the development of novel radiation-tolerant ultrasonic sensors for use in Material Testing Reactors (MTRs). As such, this test is an instrumented lead test and real-time transducer performance data is collected along with temperature and neutron and gamma flux data. The current work bridges the gap between proven out-of-pile ultrasonic techniques and in-pile deployment of ultrasonic sensors by acquiring the data necessary to demonstrate the performance of ultrasonic transducers. To date, one piezoelectric

  20. Research progress of chimeric antigen receptor-transduced-T cells in B-cell malignancies%嵌合抗原受体T细胞在B细胞性血液肿瘤中的研究进展

    Institute of Scientific and Technical Information of China (English)

    徐丽丽; 王扬

    2014-01-01

    Chimeric antigen receptor-transduced-T (CAR-T) cells shows strong cytotoxicity to the tumor with specific antigen by CTL effects and cytokines releasing without MHC-restriction.As multiple clinical trials from cancer centers around the West countries reported recent years,anti-CD19-CAR T cells can not only induce complete remission of B-cell malignancies resistant,but also eliminate the minimal residual disease (MRD) at the molecular level.It is believed that the obstacles from bench to clinic will be cleared and CAR will become one of the main cancer therapies with breakthroughs in comprehensive treatment of tumors.%嵌合抗原受体(CAR)转染的T细胞通过释放细胞因子及细胞毒性T淋巴细胞(CTL)效应可高效杀伤靶抗原阳性的肿瘤细胞,且不受相容性复合体(MHC)的限制.近年欧美多个癌症中心的临床试验表明,CD19靶向的CAR-T细胞输注不仅可使复发状态的B细胞急性淋巴细胞白血病(B-ALL)患者达完全缓解,且分子水平的微小残留病(MRD)也可转阴.其在慢性淋巴细胞白血病、B细胞性恶性淋巴瘤患者中也取得了显著疗效,相信不久的将来该技术必将成为肿瘤综合治疗的重要手段.

  1. Amiloride causes changes in the mechanical properties of hair cell bundles in the fish lateral line similar to those induced by dihydrostreptomycin

    NARCIS (Netherlands)

    Wiersinga-Post, JEC; van Netten, SM

    1998-01-01

    Amiloride is a known blocker of the mechano-electrical transduction current in sensory hair cells. Measurements of cupular motion in the lateral line organ of fish now show that amiloride concurrently changes the micromechanical properties of the hair cell bundles. The effects of amiloride on the me

  2. Ultrasound transducer assembly and method for manufacturing an ultrasound transducer assembly

    NARCIS (Netherlands)

    Dekker, R.; Henneken, V.A.; Louwerse, M.C.; Raganato, M.F.

    2015-01-01

    The present invention relates to an ultrasound transducer assembly (10), in particular for intravascular ultrasound systems. The ultrasound transducer assembly comprises at least one silicon substrate element (30) including an ultrasound transducer element (14) for emitting and receiving ultrasound

  3. Pressure transducers for cryogenic liquids

    OpenAIRE

    Pavlovskyy I. V.; Kutrakov A. P.; Maryamova I. I.; Druzhinin A. A.

    2007-01-01

    The developed universal construction of tensoresistive pressure transducer for cryogenic liquids (liquid nitrogen and liquid helium) is described. The study of strain gauges characteristics on the basis of p-type Si whiskers with different boron concentration, mounted on the invar spring elements (beams), in the wide ranges of strain ε=±1,2·10-3 and temperature 4,2-300 К for transducers simulation were carried out. It was shown that using heavily doped silicon strain gages gives the possibili...

  4. Oncostatin M and leukaemia inhibitory factor trigger signal transducer and activator of transcription 3 and extracellular signal-regulated kinase 1/2 pathways but result in heterogeneous cellular responses in trophoblast cells.

    Science.gov (United States)

    Chaiwangyen, Wittaya; Ospina-Prieto, Stephanie; Morales-Prieto, Diana M; Pereira de Sousa, Francisco Lazaro; Pastuschek, Jana; Fitzgerald, Justine S; Schleussner, Ekkehard; Markert, Udo R

    2016-04-01

    Leukaemia inhibitory factor (LIF) and oncostatin M (OSM) are pleiotropic cytokines present at the implantation site that are important for the normal development of human pregnancy. These cytokines share the cell membrane receptor subunit gp130, resulting in similar functions. The aim of this study was to compare the response to LIF and OSM in several trophoblast models with particular regard to intracellular mechanisms and invasion. Four trophoblast cell lines with different characteristics were used: HTR-8/SVneo, JEG-3, ACH-3P and AC1-M59 cells. Cells were incubated with LIF, OSM (both at 10ngmL(-1)) and the signal transducer and activator of transcription (STAT) 3 inhibitor S3I-201 (200µM). Expression and phosphorylation of STAT3 (tyr(705)) and extracellular regulated kinase (ERK) 1/2 (thr(202/204)) and the STAT3 DNA-binding capacity were analysed by Western blotting and DNA-binding assays, respectively. Cell viability and invasiveness were assessed by the methylthiazole tetrazolium salt (MTS) and Matrigel assays. Enzymatic activity of matrix metalloproteinase (MMP)-2 and MMP-9 was investigated by zymography. OSM and LIF triggered phosphorylation of STAT3 and ERK1/2, followed by a significant increase in STAT3 DNA-binding activity in all tested cell lines. Stimulation with LIF but not OSM significantly enhanced invasion of ACH-3P and JEG-3 cells, but not HTR-8/SVneo or AC1-M59 cells. Similarly, STAT3 inhibition significantly decreased the invasiveness of only ACH-3P and JEG-3 cells concomitant with decreases in secreted MMP-2 and MMP-9. OSM shares with LIF the capacity to activate ERK1/2 and STAT3 pathways in all cell lines tested, but their resulting effects are dependent on cell type. This suggests that LIF and OSM may partially substitute for each other in case of deficiencies or therapeutic interventions. PMID:25247600

  5. Proceedings of transducer 84 conference

    International Nuclear Information System (INIS)

    In the broad and varied field of sensors this conference reviews thermal sensors for temperature measurements, gas sensors for gas analysis (for example analysis of exhaust gases from vehicles), optical fiber sensors, applications for optics, mechanics, robotics and signal processing. In particular one of the applications concerns acoustical transducers operating in liquid sodium for LMFBR reactors

  6. Calculations for Piezoelectric Ultrasonic Transducers

    DEFF Research Database (Denmark)

    Jensen, Henrik

    1986-01-01

    Analysis of piezoelectric ultrasonic transducers implies a solution of a boundary value problem, for a boay which consists of different materials, including a piezoelectric part. The problem is dynamic at frequencies, where a typical wavelength is somewhat less than the size of the body. Radiation...

  7. Distinct roles of Eps8 in the maturation of cochlear and vestibular hair cells.

    Science.gov (United States)

    Tavazzani, Elisa; Spaiardi, Paolo; Zampini, Valeria; Contini, Donatella; Manca, Marco; Russo, Giancarlo; Prigioni, Ivo; Marcotti, Walter; Masetto, Sergio

    2016-07-22

    Several genetic mutations affecting the development and function of mammalian hair cells have been shown to cause deafness but not vestibular defects, most likely because vestibular deficits are sometimes centrally compensated. The study of hair cell physiology is thus a powerful direct approach to ascertain the functional status of the vestibular end organs. Deletion of Epidermal growth factor receptor pathway substrate 8 (Eps8), a gene involved in actin remodeling, has been shown to cause deafness in mice. While both inner and outer hair cells from Eps8 knockout (KO) mice showed abnormally short stereocilia, inner hair cells (IHCs) also failed to acquire mature-type ion channels. Despite the fact that Eps8 is also expressed in vestibular hair cells, Eps8 KO mice show no vestibular deficits. In the present study we have investigated the properties of vestibular Type I and Type II hair cells in Eps8-KO mice and compared them to those of cochlear IHCs. In the absence of Eps8, vestibular hair cells show normally long kinocilia, significantly shorter stereocilia and a normal pattern of basolateral voltage-dependent ion channels. We have also found that while vestibular hair cells from Eps8 KO mice show normal voltage responses to injected sinusoidal currents, which were used to mimic the mechanoelectrical transducer current, IHCs lose their ability to synchronize their responses to the stimulus. We conclude that the absence of Eps8 produces a weaker phenotype in vestibular hair cells compared to cochlear IHCs, since it affects the hair bundle morphology but not the basolateral membrane currents. This difference is likely to explain the absence of obvious vestibular dysfunction in Eps8 KO mice. PMID:27132230

  8. Irradiation Testing of Ultrasonic Transducers

    International Nuclear Information System (INIS)

    Ultrasonic technologies offer the potential for high accuracy and resolution in-pile measurement of numerous parameters, including geometry changes, temperature, crack initiation and growth, gas pressure and composition, and microstructural changes. Many Department of Energy-Office of Nuclear Energy (DOE-NE) programs are exploring the use of ultrasonic technologies to provide enhanced sensors for in-pile instrumentation during irradiation testing. For example, the ability of single, small diameter ultrasonic thermometers (UTs) to provide a temperature profile in candidate metallic and oxide fuel would provide much needed data for validating new fuel performance models. Other efforts include an ultrasonic technique to detect morphology changes (such as crack initiation and growth) and acoustic techniques to evaluate fission gas composition and pressure. These efforts are limited by the lack of existing knowledge of ultrasonic transducer material survivability under irradiation conditions. To address this need, the Pennsylvania State University (PSU) was awarded an Advanced Test Reactor National Scientific User Facility (ATR NSUF) project to evaluate promising magnetostrictive and piezoelectric transducer performance in the Massachusetts Institute of Technology Research Reactor (MITR) up to a fast fluence of at least 1021 n/cm2 (E> 0.1 MeV). This test will be an instrumented lead test; and real-time transducer performance data will be collected along with temperature and neutron and gamma flux data. By characterizing magnetostrictive and piezoelectric transducer survivability during irradiation, test results will enable the development of novel radiation tolerant ultrasonic sensors for use in Material and Test Reactors (MTRs). The current work bridges the gap between proven out-of-pile ultrasonic techniques and in-pile deployment of ultrasonic sensors by acquiring the data necessary to demonstrate the performance of ultrasonic transducers. (authors)

  9. Irradiation Testing of Ultrasonic Transducers

    Energy Technology Data Exchange (ETDEWEB)

    Daw, Joshua; Tittmann, Bernhard; Reinhardt, Brian; Kohse, Gordon E.; Ramuhalli, Pradeep; Montgomery, Robert O.; Chien, Hual-Te; Villard, Jean-Francois; Palmer, Joe; Rempe, Joy

    2014-07-30

    Ultrasonic technologies offer the potential for high accuracy and resolution in-pile measurement of a range of parameters, including geometry changes, temperature, crack initiation and growth, gas pressure and composition, and microstructural changes. Many Department of Energy-Office of Nuclear Energy (DOE-NE) programs are exploring the use of ultrasonic technologies to provide enhanced sensors for in-pile instrumentation during irradiation testing. For example, the ability of single, small diameter ultrasonic thermometers (UTs) to provide a temperature profile in candidate metallic and oxide fuel would provide much needed data for validating new fuel performance models. Other efforts include an ultrasonic technique to detect morphology changes (such as crack initiation and growth) and acoustic techniques to evaluate fission gas composition and pressure. These efforts are limited by the lack of existing knowledge of ultrasonic transducer material survivability under irradiation conditions. For this reason, the Pennsylvania State University (PSU) was awarded an Advanced Test Reactor National Scientific User Facility (ATR NSUF) project to evaluate promising magnetostrictive and piezoelectric transducer performance in the Massachusetts Institute of Technology Research Reactor (MITR) up to a fast fluence of at least 1021 n/cm2 (E> 0.1 MeV). The goal of this research is to characterize magnetostrictive and piezoelectric transducer survivability during irradiation, enabling the development of novel radiation tolerant ultrasonic sensors for use in Material and Test Reactors (MTRs). As such, this test will be an instrumented lead test and real-time transducer performance data will be collected along with temperature and neutron and gamma flux data. The current work bridges the gap between proven out-of-pile ultrasonic techniques and in-pile deployment of ultrasonic sensors by acquiring the data necessary to demonstrate the performance of ultrasonic transducers.

  10. Transducers and Arrays for Underwater Sound

    CERN Document Server

    Sherman, Charles H

    2007-01-01

    This book is concerned with the theory, development and design of electroacoustic transducers for underwater applications, and is more comprehensive than any existing book in this field. It includes the basics of the six major types of electroacoustic transducers, with emphasis on the piezoelectric ceramic transducers that are currently most widely used. It presents the basic acoustics, as well as specific acoustic data, needed in transducer design and includes analysis of nonlinear effects in transducers. A large number of specific transducer designs, including both projectors and hydrophones, are described in detail as well as methods of modeling, evaluation and measurement. Analysis of transducer arrays, including the effects of mutual radiation impedance, as well as numerical models for transducers and arrays are also covered. The book contains an extensive Appendix of useful current information, including data on the latest transduction materials, and numerous diagrams that will facilitate its use by stu...

  11. An Air-Coupled Multiple Moving Membrane Micromachined Ultrasonic Transducer With Inverse Biasing Functionality.

    Science.gov (United States)

    Emadi, Arezoo; Buchanan, Douglas A

    2016-08-01

    A novel air-coupled multiple moving membrane-capacitive micromachined ultrasonic transducer ( [Formula: see text]-CMUT) with individually biased deflectable plates has been developed. Unlike the conventional capacitive micromachined ultrasonic transducer, this device cell structure includes an additional deflectable plate that is suspended underneath the transducer top plate. This added flexible plate contributes to the device signal transmission and reception. It is demonstrated that due to the presence of this added moving plate, the transducer is capable of operating under inverse bias condition, where the driving voltage is sandwiched between two grounded electrodes. COMSOL electromechanical simulations were conducted to investigate the influence of the transducer additional moving plate. A set of three individuals and an array of [Formula: see text]-CMUT transducers were fabricated using a sacrificial technique and with resonant frequencies ranging from 0.8 to 2.1 MHz. Electrical, optical, and pitch-catch acoustic measurements were performed to characterize the transducers properties under inverse bias condition. The experimental results are shown to be in good agreement with the simulation results for all of the fabricated transducers. It is shown that these transducers are fully functional under both normal and inverse bias conditions without any degradation in the transducer performance. PMID:27254861

  12. A Low Frequency Broadband Flextensional Ultrasonic Transducer Array.

    Science.gov (United States)

    Savoia, Alessandro Stuart; Mauti, Barbara; Caliano, Giosuè

    2016-01-01

    In this paper, we propose the design and the fabrication of a multicell, piezoelectrically actuated, flextensional transducer array structure, characterized by a low mechanical impedance, thus allowing wideband and high-sensitivity immersion operation in the low ultrasonic frequency range. The transducer structure, consisting of a plurality of circular elementary cells orderly arranged according to a periodic hexagonal tiling, features a high flexibility in the definition of the active area shape and size. We investigate, by finite element modeling (FEM), the influence of different piezoelectric and elastic materials for the flexural plate, for the plate support and for the backing, on the transducer electroacoustic behavior. We carry out the dimensioning of the transducer components and cell layout, in terms of materials and geometry, respectively, by aiming at a circular active area of 80-mm diameter and broadband operation in the 30-100-kHz frequency range in immersion. PZT-5H ceramic disks and a calibrated thickness stainless steel plate are chosen for the vibrating structure, and FR-4 laminates and a brass plate, respectively, for the plate support and the backing. The diameter of the individual cells is set to 6 mm resulting in 121 cells describing a quasi-circular area, and the total thickness of the transducer is less than 10 mm. We report on the fabrication process flow for the accurate assembly of the transducer, based, respectively, on epoxy resin and wire bonding for the mechanical and electrical interconnection of the individual parts. The results of the electrical impedance and transmit pressure field characterization are finally reported and discussed. PMID:26540680

  13. Measuring Thicknesses With In Situ Ultrasonic Transducers

    Science.gov (United States)

    Dunn, Daniel E.; Cerino, Joseph R.

    1995-01-01

    Several pulsed ultrasonic transducers attached to workpiece for measurement of changes in thicknesses of workpiece at transducer locations during grinding and polishing, according to proposal. Once attached, each transducer remains attached at original position until all grinding and polishing operations complete. In typical application, workpiece glass or ceramic blank destined to become component of optical system.

  14. Power Generation Using Piezoelectric Transducer

    Directory of Open Access Journals (Sweden)

    Tanu Chouhan

    2016-05-01

    Full Text Available The most basic need of today’s world is energy which is non-renewable source of energy available on earth. The need is increasing day by day, to overcome this there is requirement of energy harvesting. This paper attempts to show how man has been utilizing and optimizing kinetic energy. Current work also illustrates the working principle of piezoelectric crystal and various sources of vibration for the crystal. “The idea of energy harvesting is applicable to sensors as well as transducers that are placed and operated on some entities for a long time to replace the sensor module batteries. Such sensors are commonly called self-powered sensors.” Embarked piezoelectric transducer, which is an electromechanical converter, undergoes mechanical vibrations therefore produce electricity. This power source has many applications as in agriculture, home application and street lighting and as energy source for sensors in remote locations.

  15. High temperature ultrasonic transducers: review

    OpenAIRE

    Kažys, R.; Voleišis, A.; Voleišienė, B.

    2008-01-01

    The problems of development of high-temperature ultrasonic transducers for modern science and technology applications are analysed. More than 10 piezoelectric materials suitable for operation at high temperatures are overviewed. It is shown that bismuth titanate based piezoelectric elements are most promisable. Bonding methods of piezoelectric elements to a protector and backing are discussed. Thermosonic gold-to-gold bonding is most modern and possesses unique features. Our achievements in t...

  16. Elongation Transducer For Tensile Tests

    Science.gov (United States)

    Roberts, Paul W.; Stokes, Thomas R.

    1994-01-01

    Extensometer transducer measures elongation of tensile-test specimen with negligible distortion of test results. Used in stress-versus-strain tests of small specimens of composite materials. Clamping stress distributed more evenly. Specimen clamped gently between jaw and facing surface of housing. Friction force of load points on conical tips onto specimen depends on compression of spring, adjusted by turning cover on housing. Limp, light nylon-insulated electrical leads impose minimal extraneous loads on measuring elements.

  17. Insulin-like growth factor 1 receptor and p38 mitogen-activated protein kinase signals inversely regulate signal transducer and activator of transcription 3 activity to control human dental pulp stem cell quiescence, propagation, and differentiation.

    Science.gov (United States)

    Vandomme, Jerome; Touil, Yasmine; Ostyn, Pauline; Olejnik, Cecile; Flamenco, Pilar; El Machhour, Raja; Segard, Pascaline; Masselot, Bernadette; Bailliez, Yves; Formstecher, Pierre; Polakowska, Renata

    2014-04-15

    Dental pulp stem cells (DPSCs) remain quiescent until activated in response to severe dental pulp damage. Once activated, they exit quiescence and enter regenerative odontogenesis, producing reparative dentin. The factors and signaling molecules that control the quiescence/activation and commitment to differentiation of human DPSCs are not known. In this study, we determined that the inhibition of insulin-like growth factor 1 receptor (IGF-1R) and p38 mitogen-activated protein kinase (p38 MAPK) signaling commonly activates DPSCs and promotes their exit from the G0 phase of the cell cycle as well as from the pyronin Y(low) stem cell compartment. The inhibition of these two pathways, however, inversely determines DPSC fate. In contrast to p38 MAPK inhibitors, IGF-1R inhibitors enhance dental pulp cell sphere-forming capacity and reduce the cells' colony-forming capacity without inducing cell death. The inverse cellular changes initiated by IGF-1R and p38 MAPK inhibitors were accompanied by inverse changes in the levels of active signal transducer and activator of transcription 3 (STAT3) factor, inactive glycogen synthase kinase 3, and matrix extracellular phosphoglycoprotein, a marker of early odontoblast differentiation. Our data suggest that there is cross talk between the IGF-1R and p38 MAPK signaling pathways in DPSCs and that the signals provided by these pathways converge at STAT3 and inversely regulate its activity to maintain quiescence or to promote self-renewal and differentiation of the cells. We propose a working model that explains the possible interactions between IGF-1R and p38 MAPK at the molecular level and describes the cellular consequences of these interactions. This model may inspire further fundamental study and stimulate research on the clinical applications of DPSC in cellular therapy and tissue regeneration. PMID:24266654

  18. Insulin-Like Growth Factor 1 Receptor and p38 Mitogen-Activated Protein Kinase Signals Inversely Regulate Signal Transducer and Activator of Transcription 3 Activity to Control Human Dental Pulp Stem Cell Quiescence, Propagation, and Differentiation

    Science.gov (United States)

    Vandomme, Jerome; Touil, Yasmine; Ostyn, Pauline; Olejnik, Cecile; Flamenco, Pilar; El Machhour, Raja; Segard, Pascaline; Masselot, Bernadette; Bailliez, Yves; Formstecher, Pierre

    2014-01-01

    Dental pulp stem cells (DPSCs) remain quiescent until activated in response to severe dental pulp damage. Once activated, they exit quiescence and enter regenerative odontogenesis, producing reparative dentin. The factors and signaling molecules that control the quiescence/activation and commitment to differentiation of human DPSCs are not known. In this study, we determined that the inhibition of insulin-like growth factor 1 receptor (IGF-1R) and p38 mitogen-activated protein kinase (p38 MAPK) signaling commonly activates DPSCs and promotes their exit from the G0 phase of the cell cycle as well as from the pyronin Ylow stem cell compartment. The inhibition of these two pathways, however, inversely determines DPSC fate. In contrast to p38 MAPK inhibitors, IGF-1R inhibitors enhance dental pulp cell sphere-forming capacity and reduce the cells' colony-forming capacity without inducing cell death. The inverse cellular changes initiated by IGF-1R and p38 MAPK inhibitors were accompanied by inverse changes in the levels of active signal transducer and activator of transcription 3 (STAT3) factor, inactive glycogen synthase kinase 3, and matrix extracellular phosphoglycoprotein, a marker of early odontoblast differentiation. Our data suggest that there is cross talk between the IGF-1R and p38 MAPK signaling pathways in DPSCs and that the signals provided by these pathways converge at STAT3 and inversely regulate its activity to maintain quiescence or to promote self-renewal and differentiation of the cells. We propose a working model that explains the possible interactions between IGF-1R and p38 MAPK at the molecular level and describes the cellular consequences of these interactions. This model may inspire further fundamental study and stimulate research on the clinical applications of DPSC in cellular therapy and tissue regeneration. PMID:24266654

  19. Enhancing endosomal escape of transduced proteins by photochemical internalisation.

    Directory of Open Access Journals (Sweden)

    Kevin Mellert

    Full Text Available Induced internalisation of functional proteins into cultured cells has become an important aspect in a rising number of in vitro and in vivo assays. The endo-lysosomal entrapment of the transduced proteins remains the major problem in all transduction protocols. In this study we compared the efficiency, cytotoxicity and protein targeting of different commercially available transduction reagents by transducing a well-studied fluorescently labelled protein (Atto488-bovine serum albumin into cultured human sarcoma cells. The amount of internalised protein and toxicity differed between the different reagents, but the percentage of transduced cells was consistently high. Furthermore, in all protocols the signals of the transduced Atto488-BSA were predominantly punctual consistent with an endosomal localisation. To overcome the endosomal entrapment, the transduction protocols were combined with a photochemical internalisation (PCI treatment. Using this combination revealed that an endosomal disruption is highly effective in cell penetrating peptide (CPP mediated transduction, whereas lipid-mediated transductions lead to a lower signal spreading throughout the cytosol. No change in the signal distribution could be achieved in treatments using non-lipid polymers as a transduction reagent. Therefore, the combination of protein transduction protocols based on CPPs with the endosomolytic treatment PCI can facilitate protein transduction experiments in vitro.

  20. CD137 is induced by the CD40 signal on chronic lymphocytic leukemia B cells and transduces the survival signal via NF-κB activation.

    Directory of Open Access Journals (Sweden)

    Yukana Nakaima

    Full Text Available CD137 is a member of the tumor necrosis factor receptor family that is expressed on activated T cells. This molecule provides a co-stimulatory signal that enhances the survival, and differentiation of cells, and has a crucial role in the development of CD8 cytotoxic T cells and anti-tumor immunity. Here we report that CD137 expression is also induced on normal or malignant human B cells by CD40 ligation by its ligand CD154. This CD137 induction was more prominent in chronic lymphocytic leukemia (CLL cells than in other types of B cells. CD137 stimulation on B cells by its ligand induced the nuclear translocation of p52 (a non-canonical NF-κB factor. In agreement with this finding, expression of the survival factor BCL-XL was upregulated. Consequently, the CD137 signal augmented the survival of CD154-stimulated CLL B cells in vitro. This unexpected induction of CD137 on B cells by CD40 signal may influence the clinical course of CLL.

  1. Transducers

    Digital Repository Service at National Institute of Oceanography (India)

    Chakraborty, B.

    stream_size 27 stream_content_type text/plain stream_name Encycl_Microcomputers_18_335.pdf.txt stream_source_info Encycl_Microcomputers_18_335.pdf.txt Content-Encoding ISO-8859-1 Content-Type text/plain; charset=ISO-8859-1 ...

  2. Recombinant Human Erythropoietin Protects Myocardial Cells from Apoptosis via the Janus-Activated Kinase 2/Signal Transducer and Activator of Transcription 5 Pathway in Rats with Epilepsy

    Directory of Open Access Journals (Sweden)

    Bao-Xin Ma, MD

    2015-12-01

    Conclusions: These results indicate that myocardial cell apoptosis may contribute to myocardial injury in epilepsy. EPO protects myocardial cells from apoptosis via the JAK2/STAT5 pathway in rats with experimental epilepsy, whereas CEPO exerts antiapoptotic activity perhaps via a pathway independent of JAK2/STAT5 signaling.

  3. Transplantation of human neural stem cells transduced with Olig2 transcription factor improves locomotor recovery and enhances myelination in the white matter of rat spinal cord following contusive injury

    Directory of Open Access Journals (Sweden)

    Suh-Kim Haeyoung

    2009-09-01

    Full Text Available Abstract Background Contusive spinal cord injury is complicated by a delayed loss of oligodendrocytes, resulting in chronic progressive demyelination. Therefore, transplantation strategies to provide oligodendrocyte lineage cells and to enhance the extent of myelination appear to be justified for spinal cord repair. The present study investigated whether transplantation of human neural stem cells (NSCs genetically modified to express Olig2 transcription factor, an essential regulator of oligodendrocyte development, can improve locomotor recovery and enhance myelination in a rat contusive spinal cord injury model. Results HB1.F3 (F3 immortalized human NSC line was transduced with a retroviral vector encoding Olig2, an essential regulator of oligodendrocyte development. Overexpression of Olig2 in human NSCs (F3.Olig2 induced activation of NKX2.2 and directed differentiation of NSCs into oligodendrocyte lineage cells in vitro. Introduction of Olig2 conferred higher proliferative activity, and a much larger number of F3.Olig2 NSCs were detected by 7 weeks after transplantation into contused spinal cord than that of parental F3 NSCs. F3.Olig2 NSCs exhibited frequent migration towards the white matter, whereas F3 NSCs were mostly confined to the gray matter or around the lesion cavities. Most of F3.Olig2 NSCs occupying the spared white matter differentiated into mature oligodendrocytes. Transplantation of F3.Olig2 NSCs increased the volume of spared white matter and reduced the cavity volume. Moreover, F3.Olig2 grafts significantly increased the thickness of myelin sheath around the axons in the spared white matter. Finally, animals with F3.Olig2 grafts showed an improvement in the quality of hindlimbs locomotion. Conclusion Transplantation of NSCs genetically modified to differentiate into an oligodendrocytic lineage may be an effective strategy to improve functional outcomes following spinal cord trauma. The present study suggests that molecular

  4. Signal transducer and activator of transcription-3 licenses Toll-like receptor 4-dependent interleukin (IL)-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells.

    Science.gov (United States)

    Cronin, J G; Kanamarlapudi, V; Thornton, C A; Sheldon, I M

    2016-09-01

    Interleukin 6 (IL-6), acting via the IL-6 receptor (IL6R) and signal transducer and activator of transcription-3 (STAT3), limits neutrophil recruitment once bacterial infections are resolved. Bovine endometritis is an exemplar mucosal disease, characterized by sustained neutrophil infiltration and elevated IL-6 and IL-8, a neutrophil chemoattractant, following postpartum Gram-negative bacterial infection. The present study examined the impact of the IL6R/STAT3 signaling pathway on IL-8 production by primary endometrial cells in response to short- or long-term exposure to lipopolysaccharide (LPS) from Gram-negative bacteria. Tyrosine phosphorylation of STAT3 is required for DNA binding and expression of specific targets genes. Immunoblotting indicated constitutive tyrosine phosphorylation of STAT3 in endometrial cells was impeded by acute exposure to LPS. After 24 h exposure to LPS, STAT3 returned to a tyrosine phosphorylated state, indicating cross-talk between the Toll-like receptor 4 (TLR4) and the IL6R/STAT3 signaling pathways. This was confirmed by short interfering RNA targeting the IL6R, which abrogated the accumulation of IL-6 and IL-8, induced by LPS. Furthermore, there was a differential endometrial cell response, as the accumulation of IL-6 and IL-8 was dependent on STAT3, suppressor of cytokine signaling 3, and Src kinase signaling in stromal cells, but not epithelial cells. In conclusion, positive feedback through the IL6R amplifies LPS-induced IL-6 and IL-8 production in the endometrium. These findings provide a mechanistic insight into how elevated IL-6 concentrations in the postpartum endometrium during bacterial infection leads to marked and sustained neutrophil infiltration. PMID:26813342

  5. Radiation-resistant pressure transducers

    International Nuclear Information System (INIS)

    Full text : The sensitive element of vibrofrequency tensor converter (VTC) is an electromechanical resonator of string type with electrostatic excitation of longitudinal mechanical vibrations. The string is made from tensosensitive thread-like monocrystal n-Ge1-x Six (length 1-5 mm, diameter 8-12 mcm) with current outlet and strictly fixed by ends at plate or deformable surface (in elastic element) at 50 mcm apartheid. With increasing Si atomic percent in n-Ge1-x Six the converter tens sensitivity increases. There has been shown the scheme of pressure transducer which contains monocrystalline silicon membrane and string tens converter from thread-like monocrystal Ge-Si. Using method, when crystal position on membrane while it deforms by pressure, corresponds to free (uptight) state, allowed to obtain the maximum sensitivity in measurement of pressure fluctuation. The transducers of absolute and pressure differential of this type can be used in automated systems of life activity. The high sensitivity of string transducers to pressure exceeding 100 hertz/mm (water column) permits to use them in devices for measuring gas concentration. The combination of optical and deformation methods of measurements forms the basis of their operation. The pressure change occurs due to the fact that gas molecules absorbing the quanta of incident light, become at excited state and then excitation energy of their vibrational-rotatory degrees of freedom converts to the energy of translational motion of molecules, i.e. to heat appropriate to pressure increase. Using these tens converters of high pressure one can prevent the possible accidents on oil pipe-like Baku-Tibilisi-Ceyhan

  6. Cochlear outer hair cells undergo an apical circumference remodeling constrained by the hair bundle shape.

    Science.gov (United States)

    Etournay, Raphaël; Lepelletier, Léa; Boutet de Monvel, Jacques; Michel, Vincent; Cayet, Nadège; Leibovici, Michel; Weil, Dominique; Foucher, Isabelle; Hardelin, Jean-Pierre; Petit, Christine

    2010-04-01

    Epithelial cells acquire diverse shapes relating to their different functions. This is particularly relevant for the cochlear outer hair cells (OHCs), whose apical and basolateral shapes accommodate the functioning of these cells as mechano-electrical and electromechanical transducers, respectively. We uncovered a circumferential shape transition of the apical junctional complex (AJC) of OHCs, which occurs during the early postnatal period in the mouse, prior to hearing onset. Geometric analysis of the OHC apical circumference using immunostaining of the AJC protein ZO1 and Fourier-interpolated contour detection characterizes this transition as a switch from a rounded-hexagon to a non-convex circumference delineating two lateral lobes at the neural side of the cell, with a negative curvature in between. This shape tightly correlates with the 'V'-configuration of the OHC hair bundle, the apical mechanosensitive organelle that converts sound-evoked vibrations into variations in cell membrane potential. The OHC apical circumference remodeling failed or was incomplete in all the mouse mutants affected in hair bundle morphogenesis that we tested. During the normal shape transition, myosin VIIa and myosin II (A and B isoforms) displayed polarized redistributions into and out of the developing lobes, respectively, while Shroom2 and F-actin transiently accumulated in the lobes. Defects in these redistributions were observed in the mutants, paralleling their apical circumference abnormalities. Our results point to a pivotal role for actomyosin cytoskeleton tensions in the reshaping of the OHC apical circumference. We propose that this remodeling contributes to optimize the mechanical coupling between the basal and apical poles of mature OHCs.

  7. PP2A1 binding, cell transducing and apoptotic properties of Vpr(77-92: a new functional domain of HIV-1 Vpr proteins.

    Directory of Open Access Journals (Sweden)

    Angélique N Godet

    Full Text Available BACKGROUND: The hallmark of HIV-1 pathogenesis is the progressive CD4(+ T cell depletion and high propensity of CD4(+ T cells to apoptosis. HIV-1 viral protein R (Vpr is a major pro-apoptotic gene product. A first Vpr-mediated apoptotic mechanism that requires a physical interaction of HIV-1 Vpr(71-82 mitochondriotoxic domain containing the conserved sequence (71-HFRIGCRHSRIG(-82 with the Adenine Nucleotide Translocator (ANT has been characterized. The family of Ser/Thr protein phosphatase PP2A interacts with several viral proteins to regulate cell growth and apoptotic pathways. Previous studies based on yeast two hybrid assays and mutational experiments indicated that PP2A(1 is involved in the induction of G2 arrest by HIV-1 Vpr. PRINCIPAL FINDINGS: Experiments combining pull-down, cell penetration and apoptosis analyses in distinct human cells indicate that the PP2A(1 binding sequence from Vpr(77-92 is a new cell penetrating apoptotic sequence. We also found that the I84P mutation or the IIQ/VTR(83-85 and T89A substitutions in the Vpr(77-92 sequence prevent PP2A(1 binding, cell penetration and apoptosis. In addition the double R77A and R80A mutation known to inactivate the mitochondriotoxic Vpr(71-82 domain, has no effect on the biological properties of the Vpr(77-92 domain. CONCLUSION: Together our data provide evidence for the first time that the Vpr(77-92 sequence delineates a biological active domain of Vpr with PP2A(1 binding and pro-apoptotic capacities and, it is conceivable that this cell penetrating sequence may account for the Vpr internalization in uninfected cells. Finally, our data also implicate the existence of two partially overlapping pro-apoptotic domains in the Vpr C-terminal part, a redundancy that represents a new approach to address the question of biological relevance of HIV-1 Vpr. In this context, future studies will be required to determine the functional relevance of the Vpr(77-92 domain in full length Vpr protein and

  8. Signal transducer and activator of transcription 5 activation is sufficient to drive transcriptional induction of cyclin D2 gene and proliferation of rat pancreatic beta-cells

    DEFF Research Database (Denmark)

    Friedrichsen, Birgitte N; Richter, Henrijette E; Hansen, Johnny A;

    2003-01-01

    cells transiently transfected with a cyclin D2 promoter-reporter construct revealed a 3- to 5-fold increase of transcriptional activity in response to hGH stimulation. Furthermore, coexpression of a constitutive active STAT5 mutant (either CA-STAT5a or CA-STAT5b) was sufficient to drive transactivation......-STAT5b stimulated transcriptional activation of the cyclin D2 promoter and induced hGH-independent proliferation in these cells. In primary beta-cells, adenovirus-mediated expression of CA-STAT5b profoundly stimulated DNA-synthesis (5.3-fold over control) in the absence of hGH. Our studies indicate...

  9. The effect of combination therapy with doxorubicin and I-131 in multidrug resistance (MDR) gene expressing cancer cells by transduced shRNA for mdr1 mRNA and sodium Iodine symporter (NIS) genes

    Energy Technology Data Exchange (ETDEWEB)

    Ahn, Sohn Joo; Lee, Yong Jin; Lee, You La; Lee, Sang Woo; Yoo, Jeong Soo; Ahn, Byeong Cheol; Lee, Jae Tae [School of Medicine, Kyungpook National University, Daegu (Korea, Republic of)

    2007-07-01

    Transduction of shMDR for mdr1 gene and NIS gene into MDR cancer cells expressing MDR can improve therapeutic effect of anticancer treatment. We have established stable cell lines expressing both shMDR and NIS gene using mammalian expression vector from human colon cancer cells having MDR characteristics. In this study, we have evaluated effects of combined therapy with doxorubicin and I-131 in xenograft model of MDR human colon cancer cells transduced with shMDR and NIS genes. We prepared adenoviruses containing shMDR (Ad-shMDR) or NIS (Ad-NIS) gene and finally established stable cell lines expressing both shMDR and NIS gene. Two days after transfection, inhibition of P-gp function by shMDR was assessed by a change of Tc-99m MIBI uptake, and functional activity of induced NIS expression was also assessed by a change of I-125 uptake. Doxorubicin and I-131 cytotoxicity was measured in Ad-shMDR transfected or non-transfected cell lines. Tc-99m MlBl and I-131 images was obtained effect in Ad-shMDR/NIS-cotransfected tumor xenograft. Dual therapy using doxorubicin and I-131 was measured effect in injected tumor xenograft by shMDR and NIS gene expressing stable cells. After transfection, uptake of Tc-99m MIBI and I-125 increased up to {approx}1.5-fold and approximately 25-fold compared to control. Ad-shMDR/NIS-cotransfected HCT15 cell showed enhanced cytotoxicity by doxorubicin and I-131 compared to control. Tc-99m MIBI and I-131 images demonstrated that in Ad-shMDR/NIS-cotransfected tumor xenograft were 2 and 10 times higher than that in non-intratumoral injected tumor xenograft. Therapy with I-131, or both doxorubicin and I-131 were revealed enhanced tumor regression than control group. Suppression of mdr1 gene expression and enhanced iodine uptake can be produced by shMDR and NIS gene transfection. Dual therapy with doxorubicin and radioiodine followed by transfection of shMDR/NIS gene can be effectively used in MDR expressing cancer cell.

  10. Transducers and arrays for underwater sound

    CERN Document Server

    Butler, John L

    2016-01-01

    This improved and updated second edition covers the theory, development, and design of electro-acoustic transducers for underwater applications. This highly regarded text discusses the basics of piezoelectric and magnetostrictive transducers that are currently being used as well as promising new designs. It presents the basic acoustics as well as the specific acoustics data needed in transducer design and evaluation. A broad range of designs of projectors and hydrophones are described in detail along with methods of modeling, evaluation, and measurement. Analysis of projector and hydrophone transducer arrays, including the effects of mutual radiation impedance and numerical models for elements and arrays, are also covered. The book includes new advances in transducer design and transducer materials and has been completely reorganized to be suitable for use as a textbook, as well as a reference or handbook. The new edition contains updates to the first edition, end-of-chapter exercises, and solutions to select...

  11. Analog circuit for controlling acoustic transducer arrays

    Energy Technology Data Exchange (ETDEWEB)

    Drumheller, Douglas S. (Cedar Crest, NM)

    1991-01-01

    A simplified ananlog circuit is presented for controlling electromechanical transducer pairs in an acoustic telemetry system. The analog circuit of this invention comprises a single electrical resistor which replaces all of the digital components in a known digital circuit. In accordance with this invention, a first transducer in a transducer pair of array is driven in series with the resistor. The voltage drop across this resistor is then amplified and used to drive the second transducer. The voltage drop across the resistor is proportional and in phase with the current to the transducer. This current is approximately 90 degrees out of phase with the driving voltage to the transducer. This phase shift replaces the digital delay required by the digital control circuit of the prior art.

  12. Mechanical and electrical characteristics of cymbal transducer

    Institute of Scientific and Technical Information of China (English)

    WANG Guangcan; ZHANG Jin; TIAN Wenjie; LIN Guoguang; LIAN Guandong; ZHANG Fuxue

    2005-01-01

    The electromechanical of Cymbal transducer has been researched. Under simple supporting condition, the mechanical and electrical characteristics have been analyzed by using Piezoelectric-elastic theory, Kirchhoff's thin shell vibration theory, Rayleigh-Ritz's theory and equivalent circuit method. The approximate solution and series resonance frequency equation have been given. Under no load, equivalent circuit, correlation parameters of cymbal transducer and the relations between the ratio of cavity depth to radius of Cymbal transducer with resonance frequency, electromechanical coupling coefficient of cymbal transducer have been researched. The best electromechanical coupling coefficient of cymbal transducer has been gained from the results of numerical analysis. It offers a valid theoretical foundation for optimum design of cymbal transducer.

  13. Silicon Integrated Cavity Optomechanical Transducer

    Science.gov (United States)

    Zou, Jie; Miao, Houxun; Michels, Thomas; Liu, Yuxiang; Srinivasan, Kartik; Aksyuk, Vladimir

    2013-03-01

    Cavity optomechanics enables measurements of mechanical motion at the fundamental limits of precision imposed by quantum mechanics. However, the need to align and couple devices to off-chip optical components hinders development, miniaturization and broader application of ultrahigh sensitivity chip-scale optomechanical transducers. Here we demonstrate a fully integrated and optical fiber pigtailed optomechanical transducer with a high Q silicon micro-disk cavity near-field coupled to a nanoscale cantilever. We detect the motion of the cantilever by measuring the resonant frequency shift of the whispering gallery mode of the micro-disk. The sensitivity near the standard quantum limit can be reached with sub-uW optical power. Our on-chip approach combines compactness and stability with great design flexibility: the geometry of the micro-disk and cantilever can be tailored to optimize the mechanical/optical Q factors and tune the mechanical frequency over two orders of magnitudes. Electrical transduction in addition to optical transduction was also demonstrated and both can be used to effectively cool the cantilever. Moreover, cantilevers with sharp tips overhanging the chip edge were fabricated to potentially allow the mechanical cantilever to be coupled to a wide range of off-chip systems, such as spins, DNA, nanostructures and atoms on clean surfaces.

  14. From Bunsen Burners to Fuel Cells: Invoking Energy Transducers to Exemplify "Paths" and Unify the Energy-Related Concepts of Thermochemistry and Thermodynamics

    Science.gov (United States)

    Hladky, Paul W.

    2009-01-01

    The conversion of chemical energy entirely into thermal energy by Bunsen burners and into thermal energy and electrical energy by fuel cells of varying efficiencies illustrates different paths by which a chemical reaction can occur. Using the efficiency of producing electrical energy as a path label allows all of the energy-related quantities to…

  15. Finite element analysis of underwater capacitor micromachined ultrasonic transducers.

    Science.gov (United States)

    Roh, Yongrae; Khuri-Yakub, Butrus T

    2002-03-01

    A simple electro-mechanical equivalent circuit model is used to predict the behavior of capacitive micromachined ultrasonic transducers (cMUT). Most often, cMUTs are made in silicon and glass plates that are in the 0.5 mm to 1 mm range in thickness. The equivalent circuit model of the cMUT lacks important features such as coupling to the substrate and the ability to predict cross-talk between elements of an array of transducers. To overcome these deficiencies, a flnite element model of the cMUT is constructed using the commercial code ANSYS. Calculation results of the complex load impedance seen by single capacitor cells are presented, then followed by a calculation of the plane wave real load impedance seen by a parallel combination of many cells that are used to make a transducer. Cross-talk between 1-D array elements is found to be due to two main sources: coupling through a Stoneley wave propagating at the transducer-water interface and coupling through Lamb waves propagating in the substrate. To reduce the cross-talk level, the effect of structural variations of the substrate are investigated, which includes a change of its thickness and etched trenches or polymer walls between array elements. PMID:12322877

  16. Evaluation of pressure transducers. Dynamic tests

    International Nuclear Information System (INIS)

    The evaluation of a pressure transducer consists in checking its specifications. The tests of rapidity with a shock tube are important because they allow to find out transducer response time under a pressure step and also its natural frequency and damping. These last two data define the whole dynamic work of a fast transducer as the accuracy of the amplitude versus frequency function, the phase rotation, limit of use, overload. Several tests carried out on ETCA shock tube are described

  17. HIGH PERFORMANCE DISULFONATED POLY(ARYLENE SULFONE) CO- AND TERPOLYMERS FOR PROTON EXCHANGE MEMBRANES FOR FUEL CELL AND TRANSDUCER APPLICATIONS: SYNTHESIS, CHARACTERIZATION AND FABRICATION OF ION CONDUCTING MEMBRANES

    OpenAIRE

    Wiles, Kenton Broyhill

    2005-01-01

    The results described in this dissertation have demonstrated several alternative proton exchange membranes (PEM) for hydrogen-air and direct methanol fuel cells (DMFC) that perform as well or better than the state of the art Nafion perfluorosulfonic acid membrane. Direct aromatic nucleophilic substitution polycondensations of disodium 3,3â S-disulfonate-4,4â S-difluorodiphenylsulfone (SDFDPS), 4,4â S-difluorodiphenylsulfone (DFDPS) (or their chlorinated analogs, SDCDPS, DCDPS) and 4,4â S...

  18. Linearization of resistance thermometers and other transducers

    DEFF Research Database (Denmark)

    Diamond, Joseph M.

    1970-01-01

    Given a resistive transducer which responds directly or indirectly to a physical quantity x, it is shown that the relationship may be linearized by linear methods if and only if both the resistance and conductance of the transducer are concave upward as functions of x. This result applies to eith...

  19. Pushdown machines for the macro tree transducer

    NARCIS (Netherlands)

    Engelfriet, Joost; Vogler, Heiko

    1986-01-01

    The macro tree transducer can be considered as a system of recursive function procedures with parameters, where the recursion is on a tree (e.g., the syntax tree of a program). We investigate characterizations of the class of tree (tree-to-string) translations which is induced by macro tree transduc

  20. Micromachined Integrated Transducers for Ultrasound Imaging

    DEFF Research Database (Denmark)

    la Cour, Mette Funding

    The purpose of this project is to develop capacitive micromachined ultrasonic transducers (CMUTs) for medical imaging. Medical ultrasound transducers used today are fabricated using piezoelectric materials and bulk processing. To fabricate transducers capable of delivering a higher imaging...... resolution it is however necessary to develop new fabrication methods that allows fabrication of transducer elements with smaller dimensions. By using microfabrication technology it is possible to push the dimensions down and provide higher design flexibility. This project is part of a large ultrasound...... project and collaboration with a lot of partners to improve medical ultrasound imaging. The focus in this part of the project is to design, fabricate and characterize 1D CMUT arrays. Two versions of 1D transducers are made, one at Stanford University and one at DTU. Electrical and acoustical...

  1. Glass-windowed ultrasound transducers.

    Science.gov (United States)

    Yddal, Tostein; Gilja, Odd Helge; Cochran, Sandy; Postema, Michiel; Kotopoulis, Spiros

    2016-05-01

    In research and industrial processes, it is increasingly common practice to combine multiple measurement modalities. Nevertheless, experimental tools that allow the co-linear combination of optical and ultrasonic transmission have rarely been reported. The aim of this study was to develop and characterise a water-matched ultrasound transducer architecture using standard components, with a central optical window larger than 10 mm in diameter allowing for optical transmission. The window can be used to place illumination or imaging apparatus such as light guides, miniature cameras, or microscope objectives, simplifying experimental setups. Four design variations of a basic architecture were fabricated and characterised with the objective to assess whether the variations influence the acoustic output. The basic architecture consisted of a piezoelectric ring and a glass disc, with an aluminium casing. The designs differed in piezoelectric element dimensions: inner diameter, ID=10 mm, outer diameter, OD=25 mm, thickness, TH=4 mm or ID=20 mm, OD=40 mm, TH=5 mm; glass disc dimensions OD=20-50 mm, TH=2-4 mm; and details of assembly. The transducers' frequency responses were characterised using electrical impedance spectroscopy and pulse-echo measurements, the acoustic propagation pattern using acoustic pressure field scans, the acoustic power output using radiation force balance measurements, and the acoustic pressure using a needle hydrophone. Depending on the design and piezoelectric element dimensions, the resonance frequency was in the range 350-630 kHz, the -6 dB bandwidth was in the range 87-97%, acoustic output power exceeded 1 W, and acoustic pressure exceeded 1 MPa peak-to-peak. 3D stress simulations were performed to predict the isostatic pressure required to induce material failure and 4D acoustic simulations. The pressure simulations indicated that specific design variations could sustain isostatic pressures up to 4.8 MPa.The acoustic simulations were able to

  2. Glass-windowed ultrasound transducers.

    Science.gov (United States)

    Yddal, Tostein; Gilja, Odd Helge; Cochran, Sandy; Postema, Michiel; Kotopoulis, Spiros

    2016-05-01

    In research and industrial processes, it is increasingly common practice to combine multiple measurement modalities. Nevertheless, experimental tools that allow the co-linear combination of optical and ultrasonic transmission have rarely been reported. The aim of this study was to develop and characterise a water-matched ultrasound transducer architecture using standard components, with a central optical window larger than 10 mm in diameter allowing for optical transmission. The window can be used to place illumination or imaging apparatus such as light guides, miniature cameras, or microscope objectives, simplifying experimental setups. Four design variations of a basic architecture were fabricated and characterised with the objective to assess whether the variations influence the acoustic output. The basic architecture consisted of a piezoelectric ring and a glass disc, with an aluminium casing. The designs differed in piezoelectric element dimensions: inner diameter, ID=10 mm, outer diameter, OD=25 mm, thickness, TH=4 mm or ID=20 mm, OD=40 mm, TH=5 mm; glass disc dimensions OD=20-50 mm, TH=2-4 mm; and details of assembly. The transducers' frequency responses were characterised using electrical impedance spectroscopy and pulse-echo measurements, the acoustic propagation pattern using acoustic pressure field scans, the acoustic power output using radiation force balance measurements, and the acoustic pressure using a needle hydrophone. Depending on the design and piezoelectric element dimensions, the resonance frequency was in the range 350-630 kHz, the -6 dB bandwidth was in the range 87-97%, acoustic output power exceeded 1 W, and acoustic pressure exceeded 1 MPa peak-to-peak. 3D stress simulations were performed to predict the isostatic pressure required to induce material failure and 4D acoustic simulations. The pressure simulations indicated that specific design variations could sustain isostatic pressures up to 4.8 MPa.The acoustic simulations were able to

  3. Electrical modeling of dielectric elastomer stack transducers

    Science.gov (United States)

    Haus, Henry; Matysek, Marc; Moessinger, Holger; Flittner, Klaus; Schlaak, Helmut F.

    2013-04-01

    Performance of dielectric elastomer transducers (DEST) depends on mechanical and electrical parameters. For designing DEST it is therefore necessary to know the influences of these parameters on the overall performance. We show an electrical equivalent circuit valid for a transducer consisting of multiple layers and derive the electrical parameters of the circuit depending on transducers geometry and surface resistivity of the electrodes. This allows describing the DESTs dynamic behavior as a function of fabrication (layout, sheet and interconnection resistance), material (breakdown strength, permittivity) and driving (voltage) parameters. Using this electrical model transfer function and cut-off frequency are calculated, describing the influence of transducer capacitance, resistance and driving frequency on the achievable actuation deflection. Furthermore non ideal boundary effects influencing the capacitance value of the transducer are investigated by an electrostatic simulation and limits for presuming a simple plate capacitor model for calculating the transducer capacitance are derived. Results provide the plate capacitor model is a valid assumption for typical transducer configurations but for certain aspect ratios of electrode dimensions to dielectric thickness -- arising e.g. in the application of tactile interfaces -- the influence of boundary effects is to be considered.

  4. Compact Orthomode Transducers Using Digital Polarization Synthesis

    CERN Document Server

    Morgan, Matthew A; Boyd, Tod A

    2010-01-01

    In this paper we present a novel class of compact orthomode transducers which use digital calibration to synthesize the desired polarization vectors while maintaining high isolation and minimizing mass and volume. These digital orthomode transducers consist of an arbitrary number of planar probes in a circular waveguide, each of which is connected to an independent receiver chain designed for stability of complex gain. The outputs of each receiver chain are then digitized and combined numerically with calibrated, complex coefficients. Measurements on two prototype digital orthomode transducers, one with three probes and one with four, show better than 50 dB polarization isolation over a 10 C temperature range with a single calibration.

  5. Hybrid piezoelectric energy harvesting transducer system

    Science.gov (United States)

    Xu, Tian-Bing (Inventor); Jiang, Xiaoning (Inventor); Su, Ji (Inventor); Rehrig, Paul W. (Inventor); Hackenberger, Wesley S. (Inventor)

    2008-01-01

    A hybrid piezoelectric energy harvesting transducer system includes: (a) first and second symmetric, pre-curved piezoelectric elements mounted separately on a frame so that their concave major surfaces are positioned opposite to each other; and (b) a linear piezoelectric element mounted separately on the frame and positioned between the pre-curved piezoelectric elements. The pre-curved piezoelectric elements and the linear piezoelectric element are spaced from one another and communicate with energy harvesting circuitry having contact points on the frame. The hybrid piezoelectric energy harvesting transducer system has a higher electromechanical energy conversion efficiency than any known piezoelectric transducer.

  6. Ultrasonic wave transducer for high temperature barrier

    International Nuclear Information System (INIS)

    This transducer is made by a metallic body pivoting on a support fixed to the barrier and an internal vitroceramic waveguide in contact on the barrier and on the other end on a piezoelectric ceramic element

  7. Performance of Honeywell silicon pressure transducers

    Digital Repository Service at National Institute of Oceanography (India)

    VijayKumar, K.; Joseph, A.; Desai, R.G.P.; Nagvekar, S.; Prabhudesai, S.; Damodaran, V.

    Simultaneous calibration of three Honeywell pressure transducers (PPTR) at four ambient temperatures have been carried out using a novel static calibration system developed in-house. Calibration results indicated that differing PPTRs exhibited...

  8. Development of pressure transducer re-instrumentating technique (II)

    International Nuclear Information System (INIS)

    Development of FP gas pressure transducer re-instrumentating technique have been conducted since 1985. The initial stage, the followings have been made : design and fabrication of re-instrumentation device and in pile performance test ; development of remote welding and fabrication technique of re-instrumentating device to irradiated fuel rod in the hot cell ; and remote inserting technique of fuel rod into capsule. In 1990, a power ramping test was carried out instrumentating two irradiated fuel rod with re-instrumentated devices respectively. As a result of performance test, it was fond that FP gas pressure transducer had been successively indicating the inner pressure corresponding to linear heat rate of fuels. At present, the power ramping test adopting these techniques acquired is on-going in JMTR (Japan Materials Testing Reactor). (author)

  9. Addition of TAT protein transduction domain and GrpE to human p53 provides soluble fusion proteins that can be transduced into dendritic cells and elicit p53-specific T-cell responses in HLA-A*0201 transgenic mice

    DEFF Research Database (Denmark)

    Justesen, S; Buus, S; Claesson, M H;

    2007-01-01

    The protein p53 has been shown to be an efficient tumour antigen in both murine and human cancer vaccine studies and cancer vaccines targeting p53 based on major histocompatibility complex (MHC) class I binding p53-derived peptides that induce cytotoxic T lymphocytes (CTLs) without p53-specific CD4......(+) T-cell help have been tested by several research groups including ours. To obtain such CD4(+) T-cell help and cover a broader repertoire of MHC haplotypes we have previously attempted to produce recombinant human p53 for vaccination purposes. However, attempts to refold a hexahis-tagged p53 protein...... in our laboratory were unsuccessful. Here, we show that fusion of an 11-amino-acid region of the human immunodeficiency virus TAT protein transduction domain (PTD) to human p53 increases the solubility of the otherwise insoluble p53 protein and this rTAT-p53 protein can be transduced into human...

  10. Development of High Frequency Miniature Ultrasound Transducers

    OpenAIRE

    Manh, Tung

    2013-01-01

    Small, high frequency (≥ 10MHz) broadband ultrasound transducers are required in modern medical imaging systems to provide short range, high resolution images for studying of microstructures in soft tissues, such as the composition of small tumors or a vessel wall. The manufacturing of these probes using conventional methods, i.e. lapping and dicing, becomes difficult and expensive for high frequency applications and there is a need to produce small ultrasound transducers with low cost and hi...

  11. Piezoelectric and acoustic materials for transducer applications

    CERN Document Server

    Safari, Ahmad

    2008-01-01

    Discusses the underlying physical principles of piezoelectric materials, important properties of ferroelectric/piezoelectric materials used in today's transducer technology, and the principles used in transducer designIncludes examples of a wide range of applications of such materials along with the appertaining rationalesProvides a comprehensive, yet concise, reference to all the pertinent aspects of piezoelectric materialsContains contributions from a select-group of distinguished researchers

  12. Design considerations for piezoelectric polymer ultrasound transducers.

    Science.gov (United States)

    Brown, L F

    2000-01-01

    Much work has been published on the design of ultrasound transducers using piezoelectric ceramics, but a great deal of this work does not apply when using the piezoelectric polymers because of their unique electrical and mechanical properties. The purpose of this paper is to review and present new insight into seven important considerations for the design of active piezoelectric polymer ultrasound transducers: piezoelectric polymer materials selection, transducer construction and packaging requirements, materials characterization and modeling, film thickness and active area design, electroding selection, backing material design, and front protection/matching layer design. Besides reviewing these design considerations, this paper also presents new insight into the design of active piezoelectric polymer ultrasonic transducers. The design and fabrication of an immersible ultrasonic transducer, which has no adhesive layer between the active element and backing layer, is included. The transducer features direct deposition of poly(vinylidene fluoride-trifluoroethylene) [P(VDF-TrFE)] copolymer onto an insulated aluminum backing substrate. Pulse-echo tests indicated a minimum insertion loss of 37 dB and -6 dB bandwidth of 9.8 to 22 MHz (71%). The use of polymer wear-protection/quarter-wave matching layers is also discussed. Test results on a P(VDF-TrFE) transducer showed that a Mylar/sup TM/ front layer provided a slight increase in pulse-echo amplitude of 15% (or 1.2 dB) and an increase in -6 dB pulse-echo fractional bandwidth from 86 to 95%. Theoretical derivations are reported for optimizing the active area of the piezoelectric polymer element for maximum power transfer at resonance. These derivations are extended to the special case for a low profile (i.e., thin) shielded transducer. A method for modeling the non-linear loading effects of a commercial pulser-receiver is also included.

  13. [Inductance transducers for borderline localization of metallic foreign bodies].

    Science.gov (United States)

    Pudov, V I; Reutov, Iu Ia; Korotkikh, S A

    1996-01-01

    The paper outlines the advantages and disadvantages of a ferroprobe inductance transducer used in the borderline localization of a foreign ferromagnetic body. To eliminate the ferroprobe transducer-inherent disadvantages, a whirl-current inductance transducer has been developed. The transducer localizes a foreign nonferromagnetic and ferromagnetic body in its borderline localization in the eye and in the whole body.

  14. High Temperature, High Power Piezoelectric Composite Transducers

    Directory of Open Access Journals (Sweden)

    Hyeong Jae Lee

    2014-08-01

    Full Text Available Piezoelectric composites are a class of functional materials consisting of piezoelectric active materials and non-piezoelectric passive polymers, mechanically attached together to form different connectivities. These composites have several advantages compared to conventional piezoelectric ceramics and polymers, including improved electromechanical properties, mechanical flexibility and the ability to tailor properties by using several different connectivity patterns. These advantages have led to the improvement of overall transducer performance, such as transducer sensitivity and bandwidth, resulting in rapid implementation of piezoelectric composites in medical imaging ultrasounds and other acoustic transducers. Recently, new piezoelectric composite transducers have been developed with optimized composite components that have improved thermal stability and mechanical quality factors, making them promising candidates for high temperature, high power transducer applications, such as therapeutic ultrasound, high power ultrasonic wirebonding, high temperature non-destructive testing, and downhole energy harvesting. This paper will present recent developments of piezoelectric composite technology for high temperature and high power applications. The concerns and limitations of using piezoelectric composites will also be discussed, and the expected future research directions will be outlined.

  15. Development of high temperature ultrasonic transducers

    International Nuclear Information System (INIS)

    Structural health monitoring (SHM) techniques are needed to maintain the reliability of power plants for long term operation. The high temperature transducers are necessary to realize SHM (monitor wall thickness of the pipings, crack growth in the materials and material evaluation) under the working condition of power plants. We have evaluated lithium niobate (LiNbO3) single crystal which is well known as a high Curie temperature piezoelectric material to develop high temperature ultrasonic transducers. The LiNbO3 was bonded onto a stainless steel substrate. The experimental transducer was heated in an electric furnace while measuring the bottom echoes from the substrate. We confirmed that the experimental high temperature transducer could work up to 1000degC. Thermal and chemical stability of LiNbO3 were confirmed using TG measurement up to 1260degC (melting point). Additionally, we have developed single and array transducers for high temperature ultrasonic measurement based on those experimental data. (author)

  16. High temperature, high power piezoelectric composite transducers.

    Science.gov (United States)

    Lee, Hyeong Jae; Zhang, Shujun; Bar-Cohen, Yoseph; Sherrit, Stewart

    2014-01-01

    Piezoelectric composites are a class of functional materials consisting of piezoelectric active materials and non-piezoelectric passive polymers, mechanically attached together to form different connectivities. These composites have several advantages compared to conventional piezoelectric ceramics and polymers, including improved electromechanical properties, mechanical flexibility and the ability to tailor properties by using several different connectivity patterns. These advantages have led to the improvement of overall transducer performance, such as transducer sensitivity and bandwidth, resulting in rapid implementation of piezoelectric composites in medical imaging ultrasounds and other acoustic transducers. Recently, new piezoelectric composite transducers have been developed with optimized composite components that have improved thermal stability and mechanical quality factors, making them promising candidates for high temperature, high power transducer applications, such as therapeutic ultrasound, high power ultrasonic wirebonding, high temperature non-destructive testing, and downhole energy harvesting. This paper will present recent developments of piezoelectric composite technology for high temperature and high power applications. The concerns and limitations of using piezoelectric composites will also be discussed, and the expected future research directions will be outlined. PMID:25111242

  17. Ultrasonic comb transducer for smart materials

    Science.gov (United States)

    Rose, J. L.

    1998-04-01

    Installation of a small multi-element comb type ultrasonic transducer is proposed as a component of a smart structure. It can be used in either an active or passive mode in carrying out ultrasonic bulk or guided wave nondestructive evaluation. Theoretical methods are developed and experimental results are presented for guided wave generation and mode control with this very efficient and versatile novel comb type ultrasonic transducer. Excitation and probe design is crucial in mode selection. The comb transducer generates waves that are influenced by such parameters as number of elements, spacing between elements, dimension, pulsing sequence, and pressure distribution. The excited elastic field depends on the excitation frequency, plate thickness, and elastic properties. Techniques are studied to optimize the applied loading and the comb transducer design parameters so that only modes that are most sensitive to particular material characteristics can be generated. Complete understanding of the comb transducer parameters and their impact on the elastic field allows us to efficiently generate higher order modes as well as low phase velocity modes which are valuable in composite material characterization. Sample experiments are presented for various plate and tube like structures.

  18. High temperature, high power piezoelectric composite transducers.

    Science.gov (United States)

    Lee, Hyeong Jae; Zhang, Shujun; Bar-Cohen, Yoseph; Sherrit, Stewart

    2014-08-08

    Piezoelectric composites are a class of functional materials consisting of piezoelectric active materials and non-piezoelectric passive polymers, mechanically attached together to form different connectivities. These composites have several advantages compared to conventional piezoelectric ceramics and polymers, including improved electromechanical properties, mechanical flexibility and the ability to tailor properties by using several different connectivity patterns. These advantages have led to the improvement of overall transducer performance, such as transducer sensitivity and bandwidth, resulting in rapid implementation of piezoelectric composites in medical imaging ultrasounds and other acoustic transducers. Recently, new piezoelectric composite transducers have been developed with optimized composite components that have improved thermal stability and mechanical quality factors, making them promising candidates for high temperature, high power transducer applications, such as therapeutic ultrasound, high power ultrasonic wirebonding, high temperature non-destructive testing, and downhole energy harvesting. This paper will present recent developments of piezoelectric composite technology for high temperature and high power applications. The concerns and limitations of using piezoelectric composites will also be discussed, and the expected future research directions will be outlined.

  19. High Temperature, High Power Piezoelectric Composite Transducers

    Science.gov (United States)

    Lee, Hyeong Jae; Zhang, Shujun; Bar-Cohen, Yoseph; Sherrit, StewarT.

    2014-01-01

    Piezoelectric composites are a class of functional materials consisting of piezoelectric active materials and non-piezoelectric passive polymers, mechanically attached together to form different connectivities. These composites have several advantages compared to conventional piezoelectric ceramics and polymers, including improved electromechanical properties, mechanical flexibility and the ability to tailor properties by using several different connectivity patterns. These advantages have led to the improvement of overall transducer performance, such as transducer sensitivity and bandwidth, resulting in rapid implementation of piezoelectric composites in medical imaging ultrasounds and other acoustic transducers. Recently, new piezoelectric composite transducers have been developed with optimized composite components that have improved thermal stability and mechanical quality factors, making them promising candidates for high temperature, high power transducer applications, such as therapeutic ultrasound, high power ultrasonic wirebonding, high temperature non-destructive testing, and downhole energy harvesting. This paper will present recent developments of piezoelectric composite technology for high temperature and high power applications. The concerns and limitations of using piezoelectric composites will also be discussed, and the expected future research directions will be outlined. PMID:25111242

  20. Quantum limit in resonant vacuum tunneling transducers

    CERN Document Server

    Onofrio, Roberto

    2010-01-01

    We propose an electromechanical transducer based on a resonant-tunneling configuration that, with respect to the standard tunneling transducers, allows larger tunneling currents while using the same bias voltage. The increased current leads to an increase of the shot noise and an increase of the momentum noise which determine the quantum limit in the system under monitoring. Experiments with micromachined masses at 4.2 K could show dominance of the momentum noise over the Brownian noise, allowing observation of the quantum-mechanical noise at the mesoscopic scale.

  1. A general digital linearising method for transducers

    NARCIS (Netherlands)

    Bolk, W.T.

    1985-01-01

    A practical three-point interpolation algorithm is presented which corrects for manufacturing tolerances, zero offset, scaling errors and nonlinearity of transducers and signal conditioners. The algorithm is applicable to data-acquisition systems which make use of a microprocessor. Each measuring ch

  2. Broadband, High-Temperature Ultrasonic Transducer

    Science.gov (United States)

    Parker, F. Raymond; Winfree, William P.; Barrows, Danny A.

    1995-01-01

    Materials chosen for endurance at high temperatures and acoustic coupling and damping. Acoustic transducer designed to exhibit broad frequency response and to survive temperatures close to melting points of brazing alloys. Attached directly and continuously to hot object monitored ultrasonically: for example, it can be attached to relatively cool spot on workpiece during brazing for taking ultrasonic quality-control measurements.

  3. Ferroelectret non-contact ultrasonic transducers

    Science.gov (United States)

    Bovtun, V.; Döring, J.; Bartusch, J.; Beck, U.; Erhard, A.; Yakymenko, Y.

    2007-09-01

    Dielectric and electromechanical properties of the cellular polypropylene ferroelectret films (EMFIT), combining strong piezoelectric response with a low density and softness, evidence their high potential for the air-coupled ultrasonic applications. The disadvantage of the low coupling factor is compensated by the extremely low acoustic impedance, which provides excellent matching to air and promises efficient sound transmission through the air transducer interface. The influence of the electrodes on the electromechanical properties was investigated. Electron beam evaporation technology was adapted to the EMFIT films, and films with both-sided Au and Al electrodes were prepared without reducing or suppressing of the electromechanical properties. Finally, prototype transducers based on the EMFIT films were developed. In spite of the simple construction and absence of matching layers, high sensitivity of the EMFIT transducers was proved in the air-coupled ultrasonic experiment. Amplitude and delay time scanned images of the polyethylene step wedge with holes, obtained in both pulse-echo and transmission modes, demonstrate that non-contact ultrasonic imaging and testing with EMFIT transducers is possible.

  4. Micromachined Ultrasonic Transducers for 3-D Imaging

    DEFF Research Database (Denmark)

    Christiansen, Thomas Lehrmann

    Real-time ultrasound imaging is a widely used technique in medical diagnostics. Recently, ultrasound systems offering real-time imaging in 3-D has emerged. However, the high complexity of the transducer probes and the considerable increase in data to be processed compared to conventional 2-D ultr...

  5. Signals transducers and activators of transcription (STAT)-induced STAT inhibitor-1 (SSI-1)/suppressor of cytokine signaling-1 (SOCS-1) suppresses tumor necrosis factor α-induced cell death in fibroblasts

    OpenAIRE

    Morita, Yoshiaki; Naka, Tetsuji; Kawazoe, Yoshinori; Fujimoto, Minoru; Narazaki, Masashi; Nakagawa, Reiko; Fukuyama, Hidehiro; Nagata, Shigekazu; Kishimoto, Tadamitsu

    2000-01-01

    Signal transducers and activators of transcription (STAT)-induced STAT inhibitor-1 [SSI-1; also known as suppressor of cytokine signaling-1 (SOCS-1)] was identified as a negative feedback regulator of Janus kinase-STAT signaling. We previously generated mice lacking the SSI-1 gene (SSI-1 −/−) and showed that thymocytes and splenocytes in SSI-1 −/− mice underwent accelerated apoptosis. In this paper, we show that murine embryonic fibroblasts lacking the SSI-1 gene are more sensitive than their...

  6. Electromechanical coupling factor of capacitive micromachined ultrasonic transducers

    Science.gov (United States)

    Caronti, Alessandro; Carotenuto, Riccardo; Pappalardo, Massimo

    2003-01-01

    Recently, a linear, analytical distributed model for capacitive micromachined ultrasonic transducers (CMUTs) was presented, and an electromechanical equivalent circuit based on the theory reported was used to describe the behavior of the transducer [IEEE Trans. Ultrason. Ferroelectr. Freq. Control 49, 159-168 (2002)]. The distributed model is applied here to calculate the dynamic coupling factor kw of a lossless CMUT, based on a definition that involves the energies stored in a dynamic vibration cycle, and the results are compared with those obtained with a lumped model. A strong discrepancy is found between the two models as the bias voltage increases. The lumped model predicts an increasing dynamic k factor up to unity, whereas the distributed model predicts a more realistic saturation of this parameter to values substantially lower. It is demonstrated that the maximum value of kw, corresponding to an operating point close to the diaphragm collapse, is 0.4 for a CMUT single cell with a circular membrane diaphragm and no parasitic capacitance (0.36 for a cell with a circular plate diaphragm). This means that the dynamic coupling factor of a CMUT is comparable to that of a piezoceramic plate oscillating in the thickness mode. Parasitic capacitance decreases the value of kw, because it does not contribute to the energy conversion. The effective coupling factor keff is also investigated, showing that this parameter coincides with kw within the lumped model approximation, but a quite different result is obtained if a computation is made with the more accurate distributed model. As a consequence, keff, which can be measured from the transducer electrical impedance, does not give a reliable value of the actual dynamic coupling factor.

  7. The CD2 isoform of protocadherin-15 is an essential component of the tip-link complex in mature auditory hair cells.

    Science.gov (United States)

    Pepermans, Elise; Michel, Vincent; Goodyear, Richard; Bonnet, Crystel; Abdi, Samia; Dupont, Typhaine; Gherbi, Souad; Holder, Muriel; Makrelouf, Mohamed; Hardelin, Jean-Pierre; Marlin, Sandrine; Zenati, Akila; Richardson, Guy; Avan, Paul; Bahloul, Amel; Petit, Christine

    2014-07-01

    Protocadherin-15 (Pcdh15) is a component of the tip-links, the extracellular filaments that gate hair cell mechano-electrical transduction channels in the inner ear. There are three Pcdh15 splice isoforms (CD1, CD2 and CD3), which only differ by their cytoplasmic domains; they are thought to function redundantly in mechano-electrical transduction during hair-bundle development, but whether any of these isoforms composes the tip-link in mature hair cells remains unknown. By immunolabelling and both morphological and electrophysiological analyses of post-natal hair cell-specific conditional knockout mice (Pcdh15ex38-fl/ex38-fl Myo15-cre+/-) that lose only this isoform after normal hair-bundle development, we show that Pcdh15-CD2 is an essential component of tip-links in mature auditory hair cells. The finding, in the homozygous or compound heterozygous state, of a PCDH15 frameshift mutation (p.P1515Tfs*4) that affects only Pcdh15-CD2, in profoundly deaf children from two unrelated families, extends this conclusion to humans. These results provide key information for identification of new components of the mature auditory mechano-electrical transduction machinery. This will also serve as a basis for the development of gene therapy for deafness caused by PCDH15 defects. PMID:24940003

  8. Parameter sensitivity study of a Field II multilayer transducer model on a convex transducer

    DEFF Research Database (Denmark)

    Bæk, David; Jensen, Jørgen Arendt; Willatzen, Morten

    2009-01-01

    A multilayer transducer model for predicting a transducer impulse response has in earlier works been developed and combined with the Field II software. This development was tested on current, voltage, and intensity measurements on piezoceramics discs (Bæk et al. IUS 2008) and a convex 128 element...... is a quantitative calibrated model for a complete ultrasound system. This includes a sensitivity study aspresented here.Statement of Contribution/MethodsThe study alters 35 different model parameters which describe a 128 element convex transducer from BK Medical Aps. The changes are within ±20 % of the values...... supplied by the manufacturer, which are considered the zero reference (ZR). Simulations of a system consisting of a transmit unit, a five material layer transducer, and the FIELD II predicted pressure are performed by altering in turn the value of a single parameter in steps of 2 %. The remaining...

  9. Ultrasound thermotherapy of breast. Theoretical design of transducer and numerical simulation of procedure

    International Nuclear Information System (INIS)

    The absorbed ultrasound energy is changed into heat in a dissipative medium. This could be of great advantage in treating tumors in a noninvasive manner, by raising their temperature to cytotoxic levels. In this study we investigated whether using a fixed-focus transducer can destroy breast tumor cells, especially in early stages, in a cost-effective manner and reduce treatment time significantly. An appropriate fixed-focus transducer was designed, and the resultant acoustic pressure was calculated by solving the Rayleigh integral. A two-dimensional breast model was constructed from the magnetic resonance image (MRI) of a cancerous breast. Then, the induced temperature elevation was calculated using the bioheat equation and applying the finite element method (FEM) to the model. Results demonstrate that this transducer can generate a temperature of up to 61 deg C in 3s, which is sufficient to destroy cancer cells, particularly in early stages. (author)

  10. Development of ultrasonic transducer for nondestructive testing of fruit

    International Nuclear Information System (INIS)

    In this study, the ultrasonic transducers for contact measurement of whole fruits were developed. The design parameters for ultrasonic transducer such as acoustical impedance of fruits, kinds of piezoelectric materials, ultrasonic wave frequency, and transducer diameter were investigated. In order to match the impedance between piezoelectric material and fruit, various wear plates were fabricated and evaluated. And to control the bandwidth of ultrasonic wave of the transducer, various backing materials were fabricated and evaluated. The wear plate of the transducer was specially designed and fabricated considering the curvature of fruit. Two kinds of transducers such as 100 kHz of central frequency with 40 mm of transducer diameter and 200 kHz of central frequency with 20 mm diameter were developed. Nondestructive evaluation of the fruit will be possible with the developed ultrasonic transducers.

  11. Mounting technique for pressure transducers minimizes measurement interferences

    Science.gov (United States)

    Lanham, R. N.; Taylor, C. E.; Balmer, C. E.; Hwang, C.

    1975-01-01

    Miniaturized transducers are fabricated from commercially available four-arm semiconductor gages; transducers are connected as bridge circuit and mounted on internal face of small diaphragm. Jacket made of conductive plastic may be needed to avoid buildup or static charges.

  12. Endoscopic Therapeutic Device Using Focused Ultrasonic Small Transducer

    Science.gov (United States)

    Yasui, Akihiro; Haga, Yoichi; Chen, Jiun-Jie; Iseki, Hiroshi; Esashi, Masayoshi; Wada, Hiroshi

    In this research, an ultrasonic probe (5.5 mm in diameter), which has a concave PZT transducer at its tip, was fabricated for ultrasonic treatments such as sonoporation and sonodynamic therapy in the human body using a catheter and/or endoscope. Ultrasound has the potential to enhance cytotoxicity of drugs such as porphyrins, a process referred to as sonodynamic therapy, and also to deliver macromolecules such as plasmid DNA, a process referred to as sonoporation. The fabricated probe was then experimentally characterized by measuring the acoustic intensity distribution around the focal point, using a PVDF needle-type ultrasonic hydrophone. When the PZT transducer was driven by a 120 Volts peak-to-peak AC signal at 1.83 MHz, the ultrasound output was successfully focused at the focal point, with a peak intensity of 24.9 W/cm2 (0.87 MPa). Using the fabricated probe, cultured Chinese Hamster Ovary (CHO) cells were exposed to ultrasound (1.83 MHz, continuous wave, peak acoustic pressure of 0.5 MPa) for 2 s in the presence of microbubbles MB-3 and Green Fluorescent Protein (GFP) plasmid DNA. As a result of sonication, the expression of GFP was observed in CHO cells.

  13. Matrine suppresses cell growth of human chronic myeloid leukemia cells via its inhibition of the interleukin-6/Janus activated kinase/signal transducer and activator of transcription 3 signaling cohort.

    Science.gov (United States)

    Ma, Lingdi; Zhu, Zhichao; Jiang, Lijia; Sun, Xiao; Lu, Xuzhang; Zhou, Min; Qian, Sixuan; Jianyong, Li

    2015-01-01

    Matrine, alkaloid isolated from Sophora flavescens, is known to be pleiotropic by exerting anti-inflammatory, anti-oxidation, as well as anti-cancer effects. However, the precise molecular targets or pathways responsible for its activities still remain unclear. The present study aimed to determine the underlying mechanisms of matrine in inhibiting the chronic myeloid leukemia cells (CML). It was observed that matrine treatment significantly suppressed CML cells proliferation, induced apoptosis and resulted in the accumulation of cells in the G0/G1 phase, accompanied by a significant decrease in Bcl-xL, Cyclin D1, and c-Myc expression. Western blot analyses revealed that matrine treatment resulted in the down-regulation in phospho-STAT3 and phospho-JAK2 without significantly effects on STAT3 and JAK2 protein levels. Matrine significantly reduced the expression of IL-6, a potent upstream activating factor of STAT3. These results strongly suggested the IL-6/JAK/STAT3 pathway play an important role in matrine's anti-leukemia effects in K562 cells.

  14. Rational Tree Morphisms and Transducer Integer Sequences: Definition and Examples

    Science.gov (United States)

    ŠUnić, Zoran

    2007-04-01

    The notion of transducer integer sequences is considered through a series of examples (the chosen examples are related to the Tower of Hanoi problem on 3 pegs). By definition, transducer integer sequences are integer sequences produced, under a suitable interpretation, by finite transducers encoding rational tree morphisms (length and prefix preserving transformations of words that have only finitely many distinct sections).

  15. Ultrasonic transducer design for uniform insonation

    International Nuclear Information System (INIS)

    Techniques used in transducer development for acoustical imaging have been evaluated for the purpose of producing broad, uniform ultrasonic fields from planar radiators. Such fields should be useful in hyperthermia, physical therapy, and ultrasonic bioeffects studies. Fourier inversion of the circ function yielded a source velocity distribution proportional to (P/r) exp ((-ik/2Z) (2Z/sup 2/+r/sup 2/)) J/sub 1/(krP/Z), where r is the radial source coordinate, k is the wave number, and P is the desired radius of uniform insonation at a depth Z in water. This source distribution can be truncated without significantly degrading the solution. A simpler solution consists of exponentially shading the edge of an otherwise uniformly excited disk transducer. This approach was successfully approximated experimentally

  16. Orbital angular momentum-entanglement frequency transducer

    CERN Document Server

    Zhou, Zhi-Yuan; Li, Yan; Ding, Dong-Sheng; Zhang, Wei; Shi, Shuai; Dong, Ming-Xin; Shi, Bao-Sen; Guo, Guang-Can

    2016-01-01

    Entanglement is a vital resource for realizing many tasks such as teleportation, secure key distribution, metrology and quantum computations. To effectively build entanglement between different quantum systems and share information between them, a frequency transducer to convert between quantum states of different wavelengths while retaining its quantum features is indispensable. Information encoded in the photons orbital angular momentum OAM degrees of freedom is preferred in harnessing the information carrying capacity of a single photon because of its unlimited dimensions. A quantum transducer, which operates at wavelengths from 1558.3 nm to 525 nm for OAM qubits, OAMpolarization hybrid entangled states, and OAM entangled states, is reported for the first time. Nonclassical properties and entanglements are demonstrated following the conversion process by performing quantum tomography, interference, and Bell inequality measurements. Our results demonstrate the capability to create an entanglement link betwe...

  17. Electromechanical transducer for acoustic telemetry system

    Energy Technology Data Exchange (ETDEWEB)

    Drumheller, Douglas S. (Cedar Crest, NM)

    1993-01-01

    An improved electromechanical transducer is provided for use in an acoustic telemetry system. The transducer of this invention comprises a stack of ferroelectric ceramic disks interleaved with a plurality of spaced electrodes which are used to electrically pole the ceramic disks. The ceramic stack is housed in a metal tubular drill collar segment. The electrodes are preferably alternatively connected to ground potential and driving potential. This alternating connection of electrodes to ground and driving potential subjects each disk to an equal electric field; and the direction of the field alternates to match the alternating direction of polarization of the ceramic disks. Preferably, a thin metal foil is sandwiched between electrodes to facilitate the electrical connection. Alternatively, a thicker metal spacer plate is selectively used in place of the metal foil in order to promote thermal cooling of the ceramic stack.

  18. Electromechanical transducer for acoustic telemetry system

    Energy Technology Data Exchange (ETDEWEB)

    Drumheller, D.S.

    1993-06-22

    An improved electromechanical transducer is provided for use in an acoustic telemetry system. The transducer of this invention comprises a stack of ferroelectric ceramic disks interleaved with a plurality of spaced electrodes which are used to electrically pole the ceramic disks. The ceramic stack is housed in a metal tubular drill collar segment. The electrodes are preferably alternatively connected to ground potential and driving potential. This alternating connection of electrodes to ground and driving potential subjects each disk to an equal electric field; and the direction of the field alternates to match the alternating direction of polarization of the ceramic disks. Preferably, a thin metal foil is sandwiched between electrodes to facilitate the electrical connection. Alternatively, a thicker metal spacer plate is selectively used in place of the metal foil in order to promote thermal cooling of the ceramic stack.

  19. Optomechanical transducers for quantum-information processing

    Energy Technology Data Exchange (ETDEWEB)

    Stannigel, K.; Zoller, P. [Institute for Quantum Optics and Quantum Information, Austrian Academy of Sciences, A-6020 Innsbruck (Austria); Institute for Theoretical Physics, University of Innsbruck, A-6020 Innsbruck (Austria); Rabl, P. [Institute for Quantum Optics and Quantum Information, Austrian Academy of Sciences, A-6020 Innsbruck (Austria); Soerensen, A. S. [QUANTOP, Niels Bohr Institute, University of Copenhagen, DK-2100 Copenhagen O (Denmark); Lukin, M. D. [Physics Department, Harvard University, Cambridge, Massachusetts 02138 (United States)

    2011-10-15

    We discuss the implementation of optical quantum networks where the interface between stationary and photonic qubits is realized by optomechanical transducers [K. Stannigel et al., Phys. Rev. Lett. 105, 220501 (2010)]. This approach does not rely on the optical properties of the qubit and thereby enables optical quantum communication applications for a wide range of solid-state spin- and charge-based systems. We present an effective description of such networks for many qubits and give a derivation of a state transfer protocol for long-distance quantum communication. We also describe how to mediate local on-chip interactions by means of the optomechanical transducers that can be used for entangling gates. We finally discuss experimental systems for the realization of our proposal.

  20. A symmetrical low temperature pressure transducer

    Science.gov (United States)

    Helvensteijn, B. P. M.; VanSciver, S. W.

    1990-03-01

    The design and operating characteristics of a fully differential pressure transducer are described. The device is intended for use with He II heat transfer experiments where it operates in vacuum and at low temperatures (Tcapacitance change to an ac output voltage. The sensitivity is roughly 5 μV/Pa. For the present application, the capacitor and electronics have acceptable performance, with a mean noise level of ±5 Pa.

  1. Orbital angular momentum-entanglement frequency transducer

    OpenAIRE

    Zhou, Zhi-Yuan; Liu, Shi-Long; Li, Yan; Ding, Dong-Sheng; Zhang, Wei; Shi, Shuai; Dong, Ming-xin; Shi, Bao-Sen; Guo, Guang-Can

    2016-01-01

    Entanglement is a vital resource for realizing many tasks such as teleportation, secure key distribution, metrology and quantum computations. To effectively build entanglement between different quantum systems and share information between them, a frequency transducer to convert between quantum states of different wavelengths while retaining its quantum features is indispensable. Information encoded in the photons orbital angular momentum OAM degrees of freedom is preferred in harnessing the ...

  2. Language of Transducer Manipulation: Codifying Terms for Effective Teaching.

    Science.gov (United States)

    Bahner, David P; Blickendorf, J Matthew; Bockbrader, Marcia; Adkins, Eric; Vira, Amar; Boulger, Creagh; Panchal, Ashish R

    2016-01-01

    There is a need for consistent, repetitive, and reliable terminology to describe the basic manipulations of the ultrasound transducer. Previously, 5 basic transducer motions have been defined and used in education. However, even with this effort, there is still a lack of consistency and clarity in describing transducer manipulation and motion. In this technical innovation, we describe an expanded definition of transducer motions, which include movements to change the transducer's angle of insonation to the target as well as the location on the body to optimize the ultrasound image. This new terminology may allow for consistent teaching and improved communication in the process of image acquisition. PMID:26679204

  3. Language of Transducer Manipulation: Codifying Terms for Effective Teaching.

    Science.gov (United States)

    Bahner, David P; Blickendorf, J Matthew; Bockbrader, Marcia; Adkins, Eric; Vira, Amar; Boulger, Creagh; Panchal, Ashish R

    2016-01-01

    There is a need for consistent, repetitive, and reliable terminology to describe the basic manipulations of the ultrasound transducer. Previously, 5 basic transducer motions have been defined and used in education. However, even with this effort, there is still a lack of consistency and clarity in describing transducer manipulation and motion. In this technical innovation, we describe an expanded definition of transducer motions, which include movements to change the transducer's angle of insonation to the target as well as the location on the body to optimize the ultrasound image. This new terminology may allow for consistent teaching and improved communication in the process of image acquisition.

  4. Folding and Function of Proteorhodopsins in Photoenergy Transducing Membranes

    Energy Technology Data Exchange (ETDEWEB)

    Spudich, John L

    2012-08-10

    The overall research objectives are to develop proteorhodopsin (PR) proteins as a model system for {alpha}-helical membrane protein insertion and folding, and to advance understanding of the diversity and mechanisms of PRs, a large family of photoenergy transducers (~4000 identified) abundant in the world’s oceans. Specific aims are: (1) To develop a highefficiency genetic selection procedure for light-driven proton-pumping in E. coli cells. Such a procedure would provide a positive selection method for proper folding and function of PRs in the E. coli membrane. (2) Characterize flash-induced absorption changes and photocurrents in PR variants in organisms from various environments, and their expression level and function when expressed in E. coli. Subaims are to: (a) elucidate the relationship of the transport mechanism to mechanisms of other microbial rhodopsins, some of which like PRs function as ion transporters and some of which use light energy to activate signaling pathways (sensory rhodopsins); and (b) identify important residues and chemical events in light-driven proton transport by PRs. In addition to their importance to the energy of the biosphere PRs have attracted interest for their potential for use in making photoenergy-transducing membranes for bioengineering applications.

  5. Safety Issues for HIFU Transducer Design

    Science.gov (United States)

    Fleury, Gérard; Berriet, Rémi; Chapelon, Jean Yves; ter Haar, Gail; Lafon, Cyril; Le Baron, Olivier; Chupin, Laurent; Pichonnat, Fabrice; Lenormand, Jérôme

    2005-03-01

    In contrast with most ultrasound modalities for medical applications, (especially ultrasound imaging), High Intensity Focused Ultrasound (HIFU) involves technologies and procedures which may present risk to the patient. These risks, resulting from the high power levels required for effective therapy, should be taken into account at the earliest stages in the design of a system dedicated to HIFU treatment. An understanding of these risks must thus be shared amongst the many players in the field of therapy using high power ultrasound. Moreover, since the number of applications of HIFU has increased appreciably over recent years and the technology is ready to move from the research to the industrial level, it is worth now considering solutions that should be put in place to guarantee the safety of the patient during HIFU treatment. This paper reports thoughts on this, identifies some risks to the patient that must be taken into consideration in the design of HIFU transducers, and proposes some solutions that could prevent the deleterious consequences of transducer misuse or failure. For the main risks identified, such as exceeding the desired acoustic power or poor control of tissue targeting, a description of transducer performance that could potentially result in problems is systematically sought. This allows proposals for precautions to be taken during operation to be made. Parameters which should be monitored to ensure safe use are also suggested. This type of approach, which should be undertaken for the different components of a therapeutic system, highlights the challenges that must be faced in the immediate future for the development and safe exploitation of HIFU systems. The necessity for standard definitions of the parameters to be checked or monitored during HIFU treatments is crucial in this approach, as is the availability of reliable dedicated measurement devices. Co-ordinated action on these topics in the HIFU community would contribute to the

  6. A high-temperature wideband pressure transducer

    Science.gov (United States)

    Zuckerwar, A. J.

    1975-01-01

    Progress in the development of a pressure transducer for measurement of the pressure fluctuations in the high temperature environment of a jet exhaust is reported. A condenser microphone carrier system was adapted to meet the specifications. A theoretical analysis is presented which describes the operation of the condenser microphone in terms of geometry, materials, and other physical properties. The analysis was used as the basis for design of a prototype high temperature microphone. The feasibility of connecting the microphone to a converter over a high temperature cable operating as a half-wavelength transmission line was also examined.

  7. Electromechanically active polymer transducers: research in Europe

    DEFF Research Database (Denmark)

    Carpi, Federico; Graz, Ingrid; Jager, Edwin;

    2013-01-01

    in two major families: ionic EAPs (activated by an electrically induced transport of ions and/or solvent) and electronic EAPs (activated by electrostatic forces). These polymers show interesting properties, such as sizable active strains and/or stresses in response to electrical driving, high mechanical...... flexibility, low density, structural simplicity, ease of processing and scalability, no acoustic noise and, in most cases, low costs. Since many of these characteristics can also describe natural muscle tissues from an engineering standpoint, it is not surprising that EAP transducers are sometimes also...

  8. Embedded ultrasonic transducers for active and passive concrete monitoring.

    Science.gov (United States)

    Niederleithinger, Ernst; Wolf, Julia; Mielentz, Frank; Wiggenhauser, Herbert; Pirskawetz, Stephan

    2015-01-01

    Recently developed new transducers for ultrasonic transmission, which can be embedded right into concrete, are now used for non-destructive permanent monitoring of concrete. They can be installed during construction or thereafter. Large volumes of concrete can be monitored for changes of material properties by a limited number of transducers. The transducer design, the main properties as well as installation procedures are presented. It is shown that compressional waves with a central frequency of 62 kHz are mainly generated around the transducer's axis. The transducer can be used as a transmitter or receiver. Application examples demonstrate that the transducers can be used to monitor concrete conditions parameters (stress, temperature, …) as well as damages in an early state or the detection of acoustic events (e.g., crack opening). Besides application in civil engineering our setups can also be used for model studies in geosciences. PMID:25923928

  9. Using FOCUS to determine the radiation impedance for square transducers

    Science.gov (United States)

    Jennings, Matthew R.; McGough, Robert J.

    2012-10-01

    The power radiated by an ultrasound transducer is calculated with the radiation resistance, which is the real part of the radiation impedance. For circular transducers, an analytical solution for the radiation impedance is known, but an analytical expression for the radiation impedance is not available for rectangular or square transducers. To determine the radiation resistance in FOCUS, the pressure on the surface of a square transducer is computed with the fast nearfield method, and then the force on the transducer face is computed by integrating the pressure. Results using this approach are numerically evaluated for a range of ka values from 0.1 to 16. The pressure on the transducer face is also computed with the Rayleigh-Sommerfeld integral, and the results are compared. The numerical value of the radiation resistance computed with FOCUS and with the Rayleigh-Sommerfeld integral converge to the same value, although FOCUS calculates the same result in about one-quarter of the time.

  10. Polyvinylidene fluoride - a polymer as transducer material

    International Nuclear Information System (INIS)

    Polyvinylidene fluoride or PVDF is a flexible, thin piezo film and long chain semicrystalline polymer containing repeated of CH/sub 2/ - CH/sub 2/. In order to obtain desired piezoelectric properties PVDF is poled by subjecting it to high electric field. In poled PVDF piezoelectric constants have different values for each axis and one constant per axis. PVDF polymer exhibits generator and motor action. Usually 1000 A /sup o/ thick vacuum-deposited aluminum electrodes are formed on both sides of the sheet. Polymer material of PVDF can be used as a sensing element with temperature range of -40 /sup 0/ C to 100 /sup 0/C and a frequency range of 10/sup -3/ to 10/sup -9/ Hz. This paper includes as experimental observation of exciting aluminium open-ended tube at its resonance using PVDF transducer and maintained in this condition using phase lock loop or PLL. The tube is free to resonate when 9-micrometer thin polyvinylidene fluoride transducer is energized. It is observed that a film of such material can easily be used to produced the requisite vibrations in the tube. (author)

  11. Biomolecular detection with a thin membrane transducer.

    Science.gov (United States)

    Cha, Misun; Shin, Jaeha; Kim, June-Hyung; Kim, Ilchaek; Choi, Junbo; Lee, Nahum; Kim, Byung-Gee; Lee, Junghoon

    2008-06-01

    We present a thin membrane transducer (TMT) that can detect nucleic acid based biomolecular reactions including DNA hybridization and protein recognition by aptamers. Specific molecular interactions on an extremely thin and flexible membrane surface cause the deflection of the membrane due to surface stress change which can be measured by a compact capacitive circuit. A gold-coated thin PDMS membrane assembled with metal patterned glass substrate is used to realize the capacitive detection. It is demonstrated that perfect match and mismatch hybridizations can be sharply discriminated with a 16-mer DNA oligonucleotide immobilized on the gold-coated surface. While the mismatched sample caused little capacitance change, the perfectly matched sample caused a well-defined capacitance decrease vs. time due to an upward deformation of the membrane by a compressive surface stress. Additionally, the TMT demonstrated the single nucleotide polymorphism (SNP) capabilities which enabled a detection of mismatching base pairs in the middle of the sequence. It is intriguing that the increase of capacitance, therefore a downward deflection due to tensile stress, was observed with the internal double mismatch hybridization. We further present the detection of thrombin protein through ligand-receptor type recognition with 15-mer thrombin aptamer as a receptor. Key aspects of this detection such as the effect of concentration variation are investigated. This capacitive thin membrane transducer presents a completely new approach for detecting biomolecular reactions with high sensitivity and specificity without molecular labelling and optical measurement. PMID:18497914

  12. Orbital Angular Momentum-Entanglement Frequency Transducer

    Science.gov (United States)

    Zhou, Zhi-Yuan; Liu, Shi-Long; Li, Yan; Ding, Dong-Sheng; Zhang, Wei; Shi, Shuai; Dong, Ming-Xin; Shi, Bao-Sen; Guo, Guang-Can

    2016-09-01

    Entanglement is a vital resource for realizing many tasks such as teleportation, secure key distribution, metrology, and quantum computations. To effectively build entanglement between different quantum systems and share information between them, a frequency transducer to convert between quantum states of different wavelengths while retaining its quantum features is indispensable. Information encoded in the photon's orbital angular momentum (OAM) degrees of freedom is preferred in harnessing the information-carrying capacity of a single photon because of its unlimited dimensions. A quantum transducer, which operates at wavelengths from 1558.3 to 525 nm for OAM qubits, OAM-polarization hybrid-entangled states, and OAM-entangled states, is reported for the first time. Nonclassical properties and entanglements are demonstrated following the conversion process by performing quantum tomography, interference, and Bell inequality measurements. Our results demonstrate the capability to create an entanglement link between different quantum systems operating in a photon's OAM degrees of freedom, which will be of great importance in building a high-capacity OAM quantum network.

  13. Instantaneous crack detection using dual PZT transducers

    Science.gov (United States)

    Kim, Seung Bum; Sohn, Hoon

    2008-03-01

    A new guided wave based nondestructive testing (NDT) technique is developed to detect crack damage in metallic plates commonly used in aircraft without using prior baseline data or a predetermined decision boundary. In conventional guided wave based techniques, damage is often identified by comparing the "current" data obtained from a potentially damaged condition of a structure with the "past" baseline data collected at the pristine condition of the structure. However, it has been reported that this type of pattern comparison with the baseline data can lead to increased false alarms due to its susceptibility to varying operational and environmental conditions of the structure. In order to tackle this issue, a reference-free damage detection technique is previously developed using two pairs of collocated lead zirconate titanate transducers (PZTs) placed on both sides of a plate. In this study, this reference-free technique is further advanced so that the PZT transducers can be placed only on one side of the specimen. Crack formation creates Lamb wave mode conversion due to a sudden change in the thickness of the structure. Then, the proposed technique instantly detects the appearance of the crack by extracting this mode conversion from the measured Lamb waves. This study suggests a reference-free statistical approach that enables damage classification using only the current data set. Numerical and experimental results are presented to demonstrate the applicability of the proposed technique to instantaneous crack detection.

  14. Embedded Ultrasonic Transducers for Active and Passive Concrete Monitoring

    OpenAIRE

    Ernst Niederleithinger; Julia Wolf; Frank Mielentz; Herbert Wiggenhauser; Stephan Pirskawetz

    2015-01-01

    Recently developed new transducers for ultrasonic transmission, which can be embedded right into concrete, are now used for non-destructive permanent monitoring of concrete. They can be installed during construction or thereafter. Large volumes of concrete can be monitored for changes of material properties by a limited number of transducers. The transducer design, the main properties as well as installation procedures are presented. It is shown that compressional waves with a central frequen...

  15. Highly reproducible Bragg grating acousto-ultrasonic contact transducers

    Science.gov (United States)

    Saxena, Indu Fiesler; Guzman, Narciso; Lieberman, Robert A.

    2014-09-01

    Fiber optic acousto-ultrasonic transducers offer numerous applications as embedded sensors for impact and damage detection in industrial and aerospace applications as well as non-destructive evaluation. Superficial contact transducers with a sheet of fiber optic Bragg gratings has been demonstrated for guided wave ultrasound based measurements. It is reported here that this method of measurement provides highly reproducible guided ultrasound data of the test composite component, despite the optical fiber transducers not being permanently embedded in it.

  16. Long-Term Stability of the NIST Conical Reference Transducer

    OpenAIRE

    Fick, Steven E.; Proctor, Thomas M.

    2011-01-01

    The National Institute of Standards and Technology (NIST) Conical Reference Transducer (CRT) is designed for purposes requiring frequency response characteristics much more uniform than those attainable with ultrasonic transducers conventionally used for acoustic emission (AE) nondestructive testing. The high performance of the CRT results from the use of design elements radically different from those of conventional transducers. The CRT was offered for sale for 15 years (1985 to 2000). Each ...

  17. Modeling of multilayered piezoelectric transducers with ultrasonic welding application

    Science.gov (United States)

    Güney, Murat; Eskinat, Esref

    2007-04-01

    Mechanical components of sandwiched piezoelectric transducers are modeled using one-dimensional wave transmission and piezoelectric equations. Using the impedance method, resonance frequencies, stress and displacement distributions along the multilayered piezoelectric transducers of different dimensions and materials are obtained. The calculated resonance frequencies and the impedances are experimentally verified. For ultrasonic welding of plastics, the effect of the parts to be welded on the resonance frequency of the whole system is investigated regarding both material damping and piezoelectric losses. Using the methods developed, several piezoelectric transducers are analysed for different designs. The obtained results can be used to better understand the qualitative relations between the design variables of ultrasonic piezoelectric transducers.

  18. Radiation endurance of piezoelectric ultrasonic transducers--a review.

    Science.gov (United States)

    Sinclair, A N; Chertov, A M

    2015-03-01

    A literature survey is presented on the radiation endurance of piezoelectric ultrasonic transducer components and complete transducer assemblies, as functions of cumulative gamma dose and neutron fluence. The most extensive data on this topic has been acquired in CANDU electrical generating stations, which use piezoelectric ultrasonic transducers manufactured commercially with minor accommodation for high radiation fields. They have been found to be reliable for cumulative gamma doses of up to approximately 2 MegaGrays; a brief summary is made of the associated accommodations required to the transducer design, and the ultimate expected failure modes. Outside of the CANDU experience, endurance data have been acquired under a diverse spectrum of operating conditions; this can impede a direct comparison of the information from different sources. Much of this data is associated with transducers immersed in liquid metal coolants associated with advanced reactor designs. Significant modifications to conventional designs have led to the availability of custom transducers that can endure well over 100 MegaGrays of cumulative gamma dose. Published data on transducer endurance against neutron fluence are reviewed, but are either insufficient, or were reported with inadequate description of test conditions, to make general conclusions on transducer endurance with high confidence. Several test projects are planned or are already underway by major laboratories and research consortia to augment the store of transducer endurance data with respect to both gamma and neutron radiation.

  19. High Temperature Ultrasonic Transducer for Real-time Inspection

    Science.gov (United States)

    Amini, Mohammad Hossein; Sinclair, Anthony N.; Coyle, Thomas W.

    A broadband ultrasonic transducer with a novel porous ceramic backing layer is introduced to operate at 700 °C. 36° Y-cut lithium niobate (LiNbO3) single crystal was selected for the piezoelectric element. By appropriate choice of constituent materials, porosity and pore size, the acoustic impedance and attenuation of a zirconia-based backing layer were optimized. An active brazing alloy with high temperature and chemical stability was selected to bond the transducer layers together. Prototype transducers have been tested at temperatures up to 700 °C. The experiments confirmed that transducer integrity was maintained.

  20. Method and results of studying conduction measuring transducers

    Energy Technology Data Exchange (ETDEWEB)

    Dunaevskii, I.G.; Korotkov, B.N.; Povkh, I.L.; Cheplyukov, V.G.

    1977-01-01

    The method and results are given for determining the sensitivity of conduction measuring transducers with a local magnetic field. The results were obtained by frequency-dependent gradation on a model pulsation velocity gauge--a thermoanemometer. The effect of measuring a transducer's diameter, inter-electrode distance and nose line forms on its spatial resolution capacity was estimated. Adjustment functions were obtained for these transducers. A concept was formulated for measuring transducers belonging to the same class. 5 references, 5 figures.

  1. Monitoring Method for the Electrical Properties of Piezoelectric Transducer

    Institute of Scientific and Technical Information of China (English)

    李文; 朱泽琪

    2012-01-01

    The monitoring of cutting force in a vibration cutting process has a great significance in the popularization of ultrasonic vibration cutting technology. A new monitoring method of which the cutting force of ultrasonic elliptic vibration cutting is monitored using the electrical properties of transducer was proposed by studying on the relationship of cutting force, transducer electric impedance and load. A measurement system was designed for the electrical properties of transducer. The feasibility of cutting force monitoring method based on the electrical properties of piezoelectric transducer was proved by the cutting experiments.

  2. Evaluation of several ultrasonic flowmeter transducers in cryogenic environment

    Science.gov (United States)

    Moughon, W. C.

    1981-04-01

    Eighteen piezoelectric ultrasonic flowmeter transducers were laboratory tested to determine their suitability and long range reliability for use by the National Transonic Facility (NTF) to measure the flow rate of 450 Kg/sec of liquid nitrogen (LN2). Tests included thermally cycling each transducer 50 to 150 times over a temperature range of 295 K (ambient) to 77 K (LN2). The transducers were submerged in liquid nitrogen for 1 to 4 hours and the signal strength and quality noted. Results disclose that the current state-of-the-art ultrasonic flow transducers are very reliable and will meet the stringent requirements of the NTF.

  3. Multi-particle trapping and manipulation by a high-frequency array transducer

    Energy Technology Data Exchange (ETDEWEB)

    Yoon, Changhan; Kang, Bong Jin; Lee, Changyang; Kim, Hyung Ham, E-mail: hyunghak@usc.edu; Shung, K. Kirk [NIH Resource Center for Medical Ultrasonic Transducer Technology and Department of Biomedical Engineering, University of Southern California, Los Angeles, California 90089 (United States)

    2014-11-24

    We report the multiple micro-particle trapping and manipulation by a single-beam acoustic tweezer using a high-frequency array transducer. A single acoustic beam generated by a 30 MHz ultrasonic linear array transducer can entrap and transport multiple micro-particles located at the main lobe and the grating lobes. The distance between trapped particles can be adjusted by changing the transmit arrangement of array-based acoustic tweezers and subsequently the location of grating lobes. The experiment results showed that the proposed method can trap and manipulate multiple particles within a range of hundreds of micrometers. Due to its simplicity and low acoustic power, which is critical to protect cells from any thermal and mechanical damages, the technique may be used for transportation of cells in cell biology, biosensors, and tissue engineering.

  4. Efferent control of the electrical and mechanical properties of hair cells in the bullfrog's sacculus.

    Directory of Open Access Journals (Sweden)

    Manuel Castellano-Muñoz

    Full Text Available BACKGROUND: Hair cells in the auditory, vestibular, and lateral-line systems respond to mechanical stimulation and transmit information to afferent nerve fibers. The sensitivity of mechanoelectrical transduction is modulated by the efferent pathway, whose activity usually reduces the responsiveness of hair cells. The basis of this effect remains unknown. METHODOLOGY AND PRINCIPAL FINDINGS: We employed immunocytological, electrophysiological, and micromechanical approaches to characterize the anatomy of efferent innervation and the effect of efferent activity on the electrical and mechanical properties of hair cells in the bullfrog's sacculus. We found that efferent fibers form extensive synaptic terminals on all macular and extramacular hair cells. Macular hair cells expressing the Ca(2+-buffering protein calretinin contain half as many synaptic ribbons and are innervated by twice as many efferent terminals as calretinin-negative hair cells. Efferent activity elicits inhibitory postsynaptic potentials in hair cells and thus inhibits their electrical resonance. In hair cells that exhibit spiking activity, efferent stimulation suppresses the generation of action potentials. Finally, efferent activity triggers a displacement of the hair bundle's resting position. CONCLUSIONS AND SIGNIFICANCE: The hair cells of the bullfrog's sacculus receive a rich efferent innervation with the heaviest projection to calretinin-containing cells. Stimulation of efferent axons desensitizes the hair cells and suppresses their spiking activity. Although efferent activation influences mechanoelectrical transduction, the mechanical effects on hair bundles are inconsistent.

  5. Influence of acoustic streaming on ultrasonic particle manipulation in a 100-well ring-transducer microplate

    Science.gov (United States)

    Ohlin, Mathias; Christakou, Athanasia E.; Frisk, Thomas; Önfelt, Björn; Wiklund, Martin

    2013-03-01

    We characterize and quantify the performance of ultrasonic particle aggregation and positioning in a 100-well microplate. We analyze the result when operating a planar ultrasonic ring transducer at different single actuation frequencies in the range 2.20-2.40 MHz, and compare with the result obtained from different schemes of frequency-modulated actuation. Compared to our previously used wedge transducer design, the ring transducer has a larger contact area facing the microplate, resulting in lower temperature increase for a given actuation voltage. Furthermore, we analyze the dynamics of acoustic streaming occurring simultaneously with the particle trapping in the wells of the microplate, and we define an adaptive ultrasonic actuation scheme for optimizing both efficiency and robustness of the method. The device is designed as a tool for ultrasound-mediated cell aggregation and positioning. This is a method for high-resolution optical characterization of time-dependent cellular processes at the level of single cells. In this paper, we demonstrate how to operate our device in order to optimize the scanning time of 3D confocal microscopy with the aim to perform high-resolution time-lapse imaging of cells or cell-cell interactions in a highly parallel manner.

  6. Linear ultrasonic motor using quadrate plate transducer

    Institute of Scientific and Technical Information of China (English)

    Jiamei JIN; Chunsheng ZHAO

    2009-01-01

    A linear ultrasonic motor using a quadrate plate transducer was developed for precision positioning. This motor consists of two pairs of Pb(Zr, Ti)O3 piezo-electric ceramic elements, which are piezoelectrically excited into the second-bending mode of the motor stator's neutral surface in two orthogonal directions, on which the tops of four projections move along an elliptical trajectory, which in turn drives a contacted slider into linear motion via frictional forces. The coincident frequency of the stator is easily obtained for its coincident characteristic dimen-sion in two orthogonal directions. The performance characteristics achieved by the motor are: 1) a maximum linear speed of more than 60 mm/s; 2) a stroke of more than 150 mm; 3) a driving force of more than 5.0 N; and 4) a response time of about 2 ms.

  7. Magnetoelastic Transducer Materials - a Plateable Possibility

    DEFF Research Database (Denmark)

    Jensen, Jens Dahl; Møller, Per

    2001-01-01

    A short presentation of the magnetostriction theory as well as a series of possible applications for magnetoelastic transducers are given. A review of the present state of development for these materials is discussed with relation to the various ways of manufacture. The paper is concluded...... as the reference material Terfenol-D were obtained, but reproduction of exact magnetic properties is still critical with the new plating technique....... with the presentation of a method for making magnetoelastic materials by electrochemical deposition (electroless) as tried by the authors in collaboration with the Daimler-Chrysler research centre in Ulm, Germany. First results of this work are both promising and intriguing: Sensitivities of the same order...

  8. Development of piezoelectric composites for transducers

    Science.gov (United States)

    Safari, A.

    1994-07-01

    For the past decade and a half, many different types of piezoelectric ceramic-polymer composites have been developed intended for transducer applications. These diphasic composites are prepared from non-active polymer, such as epoxy, and piezoelectric ceramic, such as PZT, in the form of filler powders, elongated fibers, multilayer and more complex three-dimensional structures. For the last four years, most of the efforts have been given to producing large area and fine scale PZT fiber composites. In this paper, processing of piezoelectric ceramic-polymer composites with various connectivity patterns are reviewed. Development of fine scale piezoelectric composites by lost mold, injection molding and the relic method are described. Research activities of different groups for preparing large area piezocomposites for hydrophone and actuator applications are briefly reviewed. Initial development of electrostrictive ceramics and composites are also

  9. Multilayer Array Transducer for Nonlinear Ultrasound Imaging

    Science.gov (United States)

    Owen, Neil R.; Kaczkowski, Peter J.; Li, Tong; Gross, Dan; Postlewait, Steven M.; Curra, Francesco P.

    2011-09-01

    The properties of nonlinear acoustic wave propagation are known to be able to improve the resolution of ultrasound imaging, and could be used to dynamically estimate the physical properties of tissue. However, transducers capable of launching a wave that becomes nonlinear through propagation do not typically have the necessary bandwidth to detect the higher harmonics. Here we present the design and characterization of a novel multilayer transducer for high intensity transmit and broadband receive. The transmit layer was made from a narrow-band, high-power piezoceramic (PZT), with nominal frequency of 2.0 MHz, that was diced into an array of 32 elements. Each element was 0.300 mm wide and 6.3 mm in elevation, and with a pitch of 0.400 mm the overall aperture width was 12.7 mm. A quarter-wave matching layer was attached to the PZT substrate to improve transmit efficiency and bandwidth. The overlaid receive layer was made from polyvinylidene fluoride (PVDF) that had gold metalization on one side. A custom two-sided flex circuit routed electrical connections to the PZT elements and patterned the PVDF elements; the PZT and PVDF elements had identical apertures. A low viscosity and electrically nonconductive epoxy was used for all adhesion layers. Characterization of electrical parameters and acoustic output were performed per standard methods, where transmit and receive events were driven by a software-controlled ultrasound engine. Echo data, collected from ex vivo tissue and digitized at 45 MS/s, exhibited frequency content up to the 4th harmonic of the 2 MHz transmit frequency.

  10. A predictive computational model of the kinetic mechanism of stimulus-induced transducer methylation and feedback regulation through CheY in archaeal phototaxis and chemotaxis

    Directory of Open Access Journals (Sweden)

    Oesterhelt Dieter

    2010-03-01

    Full Text Available Abstract Background Photo- and chemotaxis of the archaeon Halobacterium salinarum is based on the control of flagellar motor switching through stimulus-specific methyl-accepting transducer proteins that relay the sensory input signal to a two-component system. Certain members of the transducer family function as receptor proteins by directly sensing specific chemical or physical stimuli. Others interact with specific receptor proteins like the phototaxis photoreceptors sensory rhodopsin I and II, or require specific binding proteins as for example some chemotaxis transducers. Receptor activation by light or a change in receptor occupancy by chemical stimuli results in reversible methylation of glutamate residues of the transducer proteins. Both, methylation and demethylation reactions are involved in sensory adaptation and are modulated by the response regulator CheY. Results By mathematical modeling we infer the kinetic mechanisms of stimulus-induced transducer methylation and adaptation. The model (deterministic and in the form of ordinary differential equations correctly predicts experimentally observed transducer demethylation (as detected by released methanol in response to attractant and repellent stimuli of wildtype cells, a cheY deletion mutant, and a mutant in which the stimulated transducer species is methylation-deficient. Conclusions We provide a kinetic model for signal processing in photo- and chemotaxis in the archaeon H. salinarum suggesting an essential role of receptor cooperativity, antagonistic reversible methylation, and a CheY-dependent feedback on transducer demethylation.

  11. Front-end receiver electronics for a matrix transducer for 3-D transesophageal echocardiography.

    Science.gov (United States)

    Yu, Zili; Blaak, Sandra; Chang, Zu-yao; Yao, Jiajian; Bosch, Johan G; Prins, Christian; Lancée, Charles T; de Jong, Nico; Pertijs, Michiel A P; Meijer, Gerard C M

    2012-07-01

    There is a clear clinical need for creating 3-D images of the heart. One promising technique is the use of transesophageal echocardiography (TEE). To enable 3-D TEE, we are developing a miniature ultrasound probe containing a matrix piezoelectric transducer with more than 2000 elements. Because a gastroscopic tube cannot accommodate the cables needed to connect all transducer elements directly to an imaging system, a major challenge is to locally reduce the number of channels, while maintaining a sufficient signal-to-noise ratio. This can be achieved by using front-end receiver electronics bonded to the transducers to provide appropriate signal conditioning in the tip of the probe. This paper presents the design of such electronics, realizing time-gain compensation (TGC) and micro-beamforming using simple, low-power circuits. Prototypes of TGC amplifiers and micro-beamforming cells have been fabricated in 0.35-μm CMOS technology. These prototype chips have been combined on a printed circuit board (PCB) to form an ultrasound-receiver system capable of reading and combining the signals of three transducer elements. Experimental results show that this design is a suitable candidate for 3-D TEE.

  12. Osthole promotes differentiation into neurons and reduces neuronal apoptosis via Wnt/β-catenin signaling pathway in APP transduced neural stem cells%蛇床子素通过Wnt/β-catenin信号通路促进转染APP基因的神经干细胞分化为更多神经元且减少神经元凋亡

    Institute of Scientific and Technical Information of China (English)

    姚璎珈; 孔亮; 教亚男; 李少恒; 陶震宇; 闫宇辉; 杨静娴

    2015-01-01

    Aim To investigate the effects of osthole ( Ost) on the ability of proliferation and differentiation in APP transduced neural stem cells( NSCs) , and neu-ronal apoptosis, in order to find related mechanism. Methods A model of Alzheimer′s disease( AD) cells was successfully established by transducing APP gene into NSCs in vitro. The ability of proliferation and dif-ferentiation was tested by staining. The viability of NSCs was determined by using CCK-8 assay. The cell apoptosis was tested by Hoechst 33258 staining. The expression of GSK-3β and β-catenin mRNA was deter-mined by RT-PCR. The expression of GSK-3β and β-catenin protein was determined by Western blot. Re-sults The ability of proliferation had increased by 10 . 24% with Ost treatment, compared with APP group. The ability of differentiation had increased by 6 . 74%with Ost treatment, compared with APP group. The vi-ability of NSCs had increased and cell apoptotic rate had decreased significantly. From the results of RT-PCR and Western blot, we could find the expression of GSK-3βmRNA and protein had decreased, and the ex-pression of β-catenin mRNA and protein had increased significantly, compared with APP group. Conclusion Ost could enhance the ability of proliferation and dif-ferentiation into more neurons of NSCs transducing APP gene, and reduce neuronal apoptosis. It might be relat-ed with activiting Wnt/β-catenin signaling pathway.%目的:通过转染APP基因于神经干细胞,研究蛇床子素对其增殖和分化能力的影响,及对神经元凋亡的影响,并研究其机制。方法体外建立阿尔茨海默病的神经干细胞模型,利用免疫组化染色法,研究蛇床子素对转染APP基因的神经干细胞增殖和分化能力的影响;通过CCK-8法检测神经干细胞存活率;Hoechst 33258法检测神经元凋亡情况;RT-PCR法检测 GSK-3β和β-catenin mRNA 的表达变化情况;Western blot法检测 GSK-3β和β-catenin 蛋白的表达变化情况

  13. AXIAL DEFFLECTION STUDIES OF RING SHAPED FORCE TRANSDUCER: A REVIEW

    Directory of Open Access Journals (Sweden)

    SUDHIR KUMAR,

    2011-01-01

    Full Text Available The ring shaped force transducers are widely used in practice and are available in varying capacities from few hundred newtons to mega newtons. The present paper discusses the deflection studies of thering shaped force transducers under action of axial forces. Various methods leading to the measurement of deflection have been discussed and compared here.

  14. Quality assurance with measuring transducers through EMP planning

    International Nuclear Information System (INIS)

    Measuring transducers in industrial application are subjected to various interferences. These are discussed as well as possible protection measures. The described measuring transducers are of modular design. Thus in individual cases it is possible to prevent the interference there where it occurs through special interference protection modules. The required measuring means for the testing of the EMP are introduced. (orig.)

  15. Metal cap flexural transducers for air-coupled ultrasonics

    Science.gov (United States)

    Eriksson, T. J. R.; Dixon, S.; Ramadas, S. N.

    2015-03-01

    Ultrasonic generation and detection in fluids is inefficient due to the large difference in acoustic impedance between the piezoelectric element and the propagation medium, leading to large internal reflections and energy loss. One way of addressing the problem is to use a flexural transducer, which uses the bending modes in a thin plate or membrane. As the plate bends, it displaces the medium in front of it, hence producing sound waves. A piezoelectric flexural transducer can generate large amplitude displacements in fluid media for relatively low excitation voltages. Commercially available flexural transducers for air applications operate at 40 kHz, but there exists ultrasound applications that require significantly higher frequencies, e.g. flow measurements. Relatively little work has been done to date to understand the underlying physics of the flexural transducer, and hence how to design it to have specific properties suitable for particular applications. This paper investigates the potential of the flexural transducer and its operating principles. Two types of actuation methods are considerd: piezoelectric and electrodynamic. The piezoelectrically actuated transducer is more energy efficient and intrinsically safe, but the electrodynamic transducer has the advantage of being less sensitive to high temperature environments. The theory of vibrating plates is used to predict transducer frequency in addition to front face amplitude, which shows good correlation with experimental results.

  16. Thermal dispersion method for an ultrasonic phased-array transducer

    Science.gov (United States)

    Choi, Euna; Lee, Wonseok; Roh, Yongrae

    2016-07-01

    When the driving voltage of an ultrasonic transducer is increased to improve the quality of ultrasound images, heat is generated inside the transducer, which can burn the patient’s skin and degrade transducer performance. In this study, the method to disperse the heat inside an ultrasonic phased-array transducer has been examined. The mechanism of temperature rise due to heat generation inside the transducer was investigated by numerical analysis and the effects of the thermal properties of the components of the transducer such as specific heat and thermal conductivity on the temperature rise were analyzed. On the basis of the results, a heat-dispersive structure was devised to reduce the temperature at the surface of the acoustic lens of the transducer. Prototype transducers were fabricated to check the efficacy of the heat-dispersive structure. By experiments, we have confirmed that the new heat-dispersive structure can reduce the internal temperature by as much as 50% in comparison with the conventional structure, which confirms the validity of the thermal dispersion mechanism developed in this work.

  17. Broadband tonpilz underwater acoustic transducers based on multimode optimization

    DEFF Research Database (Denmark)

    Yao, Qingshan; Jensen, Leif Bjørnø

    1997-01-01

    Head flapping has often been considered to be deleterious for obtaining a tonpilz transducer with broadband, high power performance. In the present work, broadband, high power tonpilz transducers have been designed using the finite element (FE) method. Optimized vibrational modes including the fl...

  18. Micro-stereolithography as a transducer design method.

    Science.gov (United States)

    Ho, K S; Bradley, R J; Billson, D R; Hutchins, D A

    2008-03-01

    This paper investigates the use of micro-stereolithography, a rapid prototyping technique, in the manufacture of transducers. It is illustrated for the production of electromagnetic acoustic transducer (EMATs) coils in both meander-line and spiral configurations. A synthetic aperture focussing technique (SAFT) has been applied to the ultrasonic signals from these devices to reconstruct images in metallic objects.

  19. Embedded Ultrasonic Transducers for Active and Passive Concrete Monitoring

    Directory of Open Access Journals (Sweden)

    Ernst Niederleithinger

    2015-04-01

    Full Text Available Recently developed new transducers for ultrasonic transmission, which can be embedded right into concrete, are now used for non-destructive permanent monitoring of concrete. They can be installed during construction or thereafter. Large volumes of concrete can be monitored for changes of material properties by a limited number of transducers. The transducer design, the main properties as well as installation procedures are presented. It is shown that compressional waves with a central frequency of 62 kHz are mainly generated around the transducer’s axis. The transducer can be used as a transmitter or receiver. Application examples demonstrate that the transducers can be used to monitor concrete conditions parameters (stress, temperature, … as well as damages in an early state or the detection of acoustic events (e.g., crack opening. Besides application in civil engineering our setups can also be used for model studies in geosciences.

  20. Embedded Ultrasonic Transducers for Active and Passive Concrete Monitoring

    Science.gov (United States)

    Niederleithinger, Ernst; Wolf, Julia; Mielentz, Frank; Wiggenhauser, Herbert; Pirskawetz, Stephan

    2015-01-01

    Recently developed new transducers for ultrasonic transmission, which can be embedded right into concrete, are now used for non-destructive permanent monitoring of concrete. They can be installed during construction or thereafter. Large volumes of concrete can be monitored for changes of material properties by a limited number of transducers. The transducer design, the main properties as well as installation procedures are presented. It is shown that compressional waves with a central frequency of 62 kHz are mainly generated around the transducer’s axis. The transducer can be used as a transmitter or receiver. Application examples demonstrate that the transducers can be used to monitor concrete conditions parameters (stress, temperature, …) as well as damages in an early state or the detection of acoustic events (e.g., crack opening). Besides application in civil engineering our setups can also be used for model studies in geosciences. PMID:25923928

  1. Flexible ultrasonic array transducer for thickness measurement of curved pipes

    International Nuclear Information System (INIS)

    The feeder pipes in a Pressurized Heavy Water Reactor (PHWR) has a very complicated form with bent pipes. In this study, we have fabricated the Polyvinylidene fluoride (PVDF) array transducer to meet the dimension requirement passing smoothly along the pipe and have evaluated the signals in order to increase the accuracy of measurement. A contact array transducer was fabricated using commercially available PVDF film samples. Each pulse echo signals were acquired and analyzed using the pulser/receiver, The array transducer was demonstrated to show a serviceable performance as a contact transducer. Pulse echo reflections from a back-wall of feeder pipe were shown as a typical wideband signal. Ultrasonic signals were analyzed by considering the center frequency, band width and waveform. PVDF array transducer for thickness measurement can be applied to monitor the integrity of feeder pipes in PHWR.

  2. Piezoelectric single crystals for ultrasonic transducers in biomedical applications.

    Science.gov (United States)

    Zhou, Qifa; Lam, Kwok Ho; Zheng, Hairong; Qiu, Weibao; Shung, K Kirk

    2014-10-01

    Piezoelectric single crystals, which have excellent piezoelectric properties, have extensively been employed for various sensors and actuators applications. In this paper, the state-of-art in piezoelectric single crystals for ultrasonic transducer applications is reviewed. Firstly, the basic principles and design considerations of piezoelectric ultrasonic transducers will be addressed. Then, the popular piezoelectric single crystals used for ultrasonic transducer applications, including LiNbO3 (LN), PMN-PT and PIN-PMN-PT, will be introduced. After describing the preparation and performance of the single crystals, the recent development of both the single-element and array transducers fabricated using the single crystals will be presented. Finally, various biomedical applications including eye imaging, intravascular imaging, blood flow measurement, photoacoustic imaging, and microbeam applications of the single crystal transducers will be discussed. PMID:25386032

  3. Calibration of Field II using a Convex Ultrasound Transducer

    DEFF Research Database (Denmark)

    Bæk, David; Jensen, Jørgen Arendt; Willatzen, Morten

    2010-01-01

    BK-Medical (Herlev, Denmark). As input waveform for the Field model we measured the output voltage of the research amplifier, which peak voltage was limited to 31 V to avoid too high non linear effects. We measured the hydrophone output from three transducer front elements by averaging 40 shoot......Field II is an ultrasound simulation program capable of simulating the pressure scattering from inhomogeneous tissue. The simulations are based on a convolution between spatial impulse responses from the field in front of the transducer and the volt-to-surface acceleration impulse response...... of the transducer. For such simulations to reflect actual measured intensities and pressure levels, the transducer impulse response is to be known. This work presents the results of combining a modified form of a 1D linear transducer model originally suggested by Willatzen with the Field II program to calibrate...

  4. Design of matching layers for high-frequency ultrasonic transducers

    Science.gov (United States)

    Fei, Chunlong; Ma, Jianguo; Chiu, Chi Tat; Williams, Jay A.; Fong, Wayne; Chen, Zeyu; Zhu, BenPeng; Xiong, Rui; Shi, Jing; Hsiai, Tzung K.; Shung, K. Kirk; Zhou, Qifa

    2015-09-01

    Matching the acoustic impedance of high-frequency (≥100 MHz) ultrasound transducers to an aqueous loading medium remains a challenge for fabricating high-frequency transducers. The traditional matching layer design has been problematic to establish high matching performance given requirements on both specific acoustic impedance and precise thickness. Based on both mass-spring scheme and microwave matching network analysis, we interfaced metal-polymer layers for the matching effects. Both methods hold promises for guiding the metal-polymer matching layer design. A 100 MHz LiNbO3 transducer was fabricated to validate the performance of the both matching layer designs. In the pulse-echo experiment, the transducer echo amplitude increased by 84.4% and its -6dB bandwidth increased from 30.2% to 58.3% comparing to the non-matched condition, demonstrating that the matching layer design method is effective for developing high-frequency ultrasonic transducers.

  5. Influence of acoustic streaming on ultrasonic particle manipulation in a 100-well ring-transducer microplate

    International Nuclear Information System (INIS)

    We characterize and quantify the performance of ultrasonic particle aggregation and positioning in a 100-well microplate. We analyze the result when operating a planar ultrasonic ring transducer at different single actuation frequencies in the range 2.20–2.40 MHz, and compare with the result obtained from different schemes of frequency-modulated actuation. Compared to our previously used wedge transducer design, the ring transducer has a larger contact area facing the microplate, resulting in lower temperature increase for a given actuation voltage. Furthermore, we analyze the dynamics of acoustic streaming occurring simultaneously with the particle trapping in the wells of the microplate, and we define an adaptive ultrasonic actuation scheme for optimizing both efficiency and robustness of the method. The device is designed as a tool for ultrasound-mediated cell aggregation and positioning. This is a method for high-resolution optical characterization of time-dependent cellular processes at the level of single cells. In this paper, we demonstrate how to operate our device in order to optimize the scanning time of 3D confocal microscopy with the aim to perform high-resolution time-lapse imaging of cells or cell–cell interactions in a highly parallel manner. (paper)

  6. Controlled inflation of voids in cellular polymer ferroelectrets: Optimizing electromechanical transducer properties

    Science.gov (United States)

    Wegener, M.; Wirges, W.; Gerhard-Multhaupt, R.; Dansachmüller, M.; Schwödiauer, R.; Bauer-Gogonea, S.; Bauer, S.; Paajanen, M.; Minkkinen, H.; Raukola, J.

    2004-01-01

    When exposed to sufficiently high electric fields, polymer-foam electret materials with closed cells exhibit ferroelectric-like behavior and may therefore be called ferroelectrets. In cellular ferroelectrets, the influence of the cell size and shape distributions on the application-relevant properties is not yet understood. Therefore, controlled inflation experiments were carried out on cellular polypropylene films, and the resulting elastical and electromechanical parameters were determined. The elastic modulus in the thickness direction shows a minimum with a corresponding maximum in the electromechanical transducer coefficient. The resonance frequency shifts as a function of the elastic modulus and the relative density of the inflated cellular films. Therefore, the transducer properties of cellular ferroelectrets can be optimized by means of controlled inflation.

  7. Transplantation of human neural stem cells transduced with Olig2 transcription factor improves locomotor recovery and enhances myelination in the white matter of rat spinal cord following contusive injury

    OpenAIRE

    Suh-Kim Haeyoung; Joo In S; Lee Seung I; Kim Eun J; Kim Byung G; Hwang Dong H; Sohn Seonghyang; Kim Seung U

    2009-01-01

    Abstract Background Contusive spinal cord injury is complicated by a delayed loss of oligodendrocytes, resulting in chronic progressive demyelination. Therefore, transplantation strategies to provide oligodendrocyte lineage cells and to enhance the extent of myelination appear to be justified for spinal cord repair. The present study investigated whether transplantation of human neural stem cells (NSCs) genetically modified to express Olig2 transcription factor, an essential regulator of olig...

  8. Interferon-γ-induced activation of Signal Transducer and Activator of Transcription 1 (STAT1 up-regulates the tumor suppressing microRNA-29 family in melanoma cells

    Directory of Open Access Journals (Sweden)

    Schmitt Martina J

    2012-12-01

    Full Text Available Abstract Background The type-II-cytokine IFN-γ is a pivotal player in innate immune responses but also assumes functions in controlling tumor cell growth by orchestrating cellular responses against neoplastic cells. The role of IFN-γ in melanoma is not fully understood: it is a well-known growth inhibitor of melanoma cells in vitro. On the other hand, IFN-γ may also facilitate melanoma progression. While interferon-regulated genes encoding proteins have been intensively studied since decades, the contribution of miRNAs to effects mediated by interferons is an emerging area of research. We recently described a distinct and dynamic regulation of a whole panel of microRNAs (miRNAs after IFN-γ-stimulation. The aim of this study was to analyze the transcriptional regulation of miR-29 family members in detail, identify potential interesting target genes and thus further elucidate a potential signaling pathway IFN-γ → Jak→ P-STAT1 → miR-29 → miR-29 target genes and its implication for melanoma growth. Results Here we show that IFN-γ induces STAT1-dependently a profound up-regulation of the miR-29 primary cluster pri-29a~b-1 in melanoma cell lines. Furthermore, expression levels of pri-29a~b-1 and mature miR-29a and miR-29b were elevated while the pri-29b-2~c cluster was almost undetectable. We observed an inverse correlation between miR-29a/b expression and the proliferation rate of various melanoma cell lines. This finding could be corroborated in cells transfected with either miR-29 mimics or inhibitors. The IFN-γ-induced G1-arrest of melanoma cells involves down-regulation of CDK6, which we proved to be a direct target of miR-29 in these cells. Compared to nevi and normal skin, and metastatic melanoma samples, miR-29a and miR-29b levels were found strikingly elevated in certain patient samples derived from primary melanoma. Conclusions Our findings reveal that the miR-29a/b1 cluster is to be included in the group of IFN- and STAT

  9. Design of advanced ultrasonic transducers for welding devices.

    Science.gov (United States)

    Parrini, L

    2001-11-01

    A new high frequency ultrasonic transducer has been conceived, designed, prototyped, and tested. In the design phase, an advanced approach was used and established. The method is based on an initial design estimate obtained with finite element method (FEM) simulations. The simulated ultrasonic transducers and resonators are then built and characterized experimentally through laser interferometry and electrical resonance spectra. The comparison of simulation results with experimental data allows the parameters of FEM models to be adjusted and optimized. The achieved FEM simulations exhibit a remarkably high predictive potential and allow full control of the vibration behavior of the transducer. The new transducer is mounted on a wire bonder with a flange whose special geometry was calculated by means of FEM simulations. This flange allows the transducer to be attached on the wire bonder, not only in longitudinal nodes, but also in radial nodes of the ultrasonic field excited in the horn. This leads to a total decoupling of the transducer to the wire bonder, which has not been achieved so far. The new approach to mount ultrasonic transducers on a welding device is of major importance, not only for wire bonding, but also for all high power ultrasound applications and has been patented.

  10. Flexible ultrasonic transducers for structural health monitoring

    International Nuclear Information System (INIS)

    Flexible ultrasonic transducers (FUTs) which have on-site installation capability are presented for non-destructive evaluation (NDE) and structural health monitoring (SHM) purposes. These FUTs typically consist of a 70 μm thick piezoelectric lead-zirconate-titanate (PZT) composite (PZT-c) coated by a sol-gel spray technique on a 75 μm thick titanium (Ti) membrane. Such an FUT was glued onto a steel pipe of 101 mm in diameter and 4.5 mm in wall thickness and heated at up to 200oC with the glue serving as a high temperature ultrasonic couplant. The pipe thickness measurement accuracy at 200oC is estimated to be 13 μm. FUTs were also glued onto the end edge of a 2 mm thick aluminum (Al) plate to generate and receive predominantly shear-horizontal (SH) plate acoustic waves (PAWs) to detect simulated line defects at temperatures of up to 100oC. FUTs, glued onto a graphite/epoxy (Gr/Ep) composite plate, were also used for the detection of an artificial disbond. An induction type non-contact method for the evaluation of Al plates and Gr/Ep composites using FUTs is also demonstrated. (author)

  11. Ultrasonic Transducer Peak-to-Peak Optical Measurement

    Directory of Open Access Journals (Sweden)

    Pavel Skarvada

    2012-01-01

    Full Text Available Possible optical setups for measurement of the peak-to-peak value of an ultrasonic transducer are described in this work. The Michelson interferometer with the calibrated nanopositioner in reference path and laser Doppler vibrometer were used for the basic measurement of vibration displacement. Langevin type of ultrasonic transducer is used for the purposes of Electro-Ultrasonic Nonlinear Spectroscopy (EUNS. Parameters of produced mechanical vibration have to been well known for EUNS. Moreover, a monitoring of mechanical vibration frequency shift with a mass load and sample-transducer coupling is important for EUNS measurement.

  12. Dynamic mechanism and its modelling of micromachined electrostatic ultrasonic transducers

    Institute of Scientific and Technical Information of China (English)

    葛立峰

    1999-01-01

    A tensile-plate-on-air-spring model (or called TDK model for short) for micromachined electrostatic ultrasonic transducers has been developed based on a thorough investigation of their dynamic mechanism. The mechanical stiffness effects caused by the compressibility of air gaps, bending stiffness of the diaphragm and in-plane tension applied to the diaphragm, together with an electrostatic negative stiffness effect are included completely in the model. Desired particular fundamental frequency and bandwidth can be obtained by only properly tailoring the geometry, dimensions and materials of transducers according to the model, which provides thereby a reliable theoretical basis for the understanding and optimised design of such transducers.

  13. Inter Digital Transducer Modelling through Mason Equivalent Circuit Model

    DEFF Research Database (Denmark)

    Mishra, Dipti; Singh, Abhishek; Hussain, Dil muhammed Akbar;

    2016-01-01

    by Simulation program with Integrated Circuit Emphasis (HSPICE), a well-liked electronic path simulator. The acoustic wave devices are not suitable to simulation through circuit simulator.In this paper, an electrical model of Mason’s Equivalent electricalcircuit for an inter-digital transducer (IDT......The frequency reliance of inter-digital transducer is analyzed with the help of MASON’s Equivalent circuit which is based on Smith’s Equivalent circuit which is further based on Foster’sNetwork. An inter-digital transducer has been demonstratedas a RLC network. The circuit is simulated...

  14. A thermal insulation method for a piezoelectric transducer

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This study deals with the sources of signal distortion of a piezoelectric transducer heated by measured gas flow. These signal distortions originate from both unloading of preload on a piezocrystal because of expansion of a diaphragm in the test apparatus and the pyroelectric effect of a heated piezoelectric crystal. A plastic film on the diaphragm of the transducer can effectively insulate the diaphragm and the piezocrystal within transducer from heating by gas flow, eliminating the sources of distortion. A method for evaluating the thickness of the film is proposed.

  15. Optimization of ultrasonic transducers for selective guided wave actuation

    Science.gov (United States)

    Miszczynski, Mateusz; Packo, Pawel; Zbyrad, Paulina; Stepinski, Tadeusz; Uhl, Tadeusz; Lis, Jerzy; Wiatr, Kazimierz

    2016-04-01

    The application of guided waves using surface-bonded piezoceramic transducers for nondestructive testing (NDT) and Structural Health Monitoring (SHM) have shown great potential. However, due to difficulty in identification of individual wave modes resulting from their dispersive and multi-modal nature, selective mode excitement methods are highly desired. The presented work focuses on an optimization-based approach to design of a piezoelectric transducer for selective guided waves generation. The concept of the presented framework involves a Finite Element Method (FEM) model in the optimization process. The material of the transducer is optimized in topological sense with the aim of tuning piezoelectric properties for actuation of specific guided wave modes.

  16. USE OF PELTIER COOLERS AS SOIL HEAT FLUX TRANSDUCERS.

    Science.gov (United States)

    Weaver, H.L.; Campbell, G.S.

    1985-01-01

    Peltier coolers were modified and calibrated to serve as soil heat flux transducers. The modification was to fill their interiors with epoxy. The average calibration constant on 21 units was 13. 6 plus or minus 0. 8 kW m** minus **2 V** minus **1 at 20 degree C. This sensitivity is about eight times that of the two thermopile transducers with which comparisons were made. The thermal conductivity of the Peltier cooler transducers was 0. 4 W m** minus **1 degree C** minus **1, which is comparable to that of dry soil.

  17. Electromechanically active polymer transducers: research in Europe

    Science.gov (United States)

    Carpi, Federico; Graz, Ingrid; Jager, Edwin; Ladegaard Skov, Anne; Vidal, Frédéric

    2013-10-01

    Smart materials and structures based on electromechanically active polymers (EAPs) represent a fast growing and stimulating field of research and development. EAPs are materials capable of changing dimensions and/or shape in response to suitable electrical stimuli. They are commonly classified in two major families: ionic EAPs (activated by an electrically induced transport of ions and/or solvent) and electronic EAPs (activated by electrostatic forces). These polymers show interesting properties, such as sizable active strains and/or stresses in response to electrical driving, high mechanical flexibility, low density, structural simplicity, ease of processing and scalability, no acoustic noise and, in most cases, low costs. Since many of these characteristics can also describe natural muscle tissues from an engineering standpoint, it is not surprising that EAP transducers are sometimes also referred to as 'muscle-like smart materials' or 'artificial muscles'. They are used not only to generate motion, but also to sense or harvest energy from it. In particular, EAP electromechanical transducers are studied for applications that can benefit from their 'biomimetic' characteristics, with possible usages from the micro- to the macro-scale, spanning several disciplines, such as mechatronics, robotics, automation, biotechnology and biomedical engineering, haptics, fluidics, optics and acoustics. Currently, the EAP field is just undergoing its initial transition from academic research into commercialization, with companies starting to invest in this technology and the first products appearing on the market. This focus issue is intentionally aimed at gathering contributions from the most influential European groups working in the EAP field. In fact, today Europe hosts the broadest EAP community worldwide. The rapid expansion of the EAP field in Europe, where it historically has strong roots, has stimulated the creation of the 'European Scientific Network for Artificial

  18. Smoothened transduces Hedgehog signal by forming a complex with Evc/Evc2.

    Science.gov (United States)

    Yang, Cuiping; Chen, Wenlin; Chen, Yongbin; Jiang, Jin

    2012-11-01

    Hedgehog (Hh) signaling plays pivotal roles in embryonic development and adult tissue homeostasis in species ranging from Drosophila to mammals. The Hh signal is transduced by Smoothened (Smo), a seven-transmembrane protein related to G protein coupled receptors. Despite a conserved mechanism by which Hh activates Smo in Drosophila and mammals, how mammalian Hh signal is transduced from Smo to the Gli transcription factors is poorly understood. Here, we provide evidence that two ciliary proteins, Evc and Evc2, the products of human disease genes responsible for the Ellis-van Creveld syndrome, act downstream of Smo to transduce the Hh signal. We found that loss of Evc/Evc2 does not affect Sonic Hedgehog-induced Smo phosphorylation and ciliary localization but impedes Hh pathway activation mediated by constitutively active forms of Smo. Evc/Evc2 are dispensable for the constitutive Gli activity in Sufu(-/-) cells, suggesting that Evc/Evc2 act upstream of Sufu to promote Gli activation. Furthermore, we demonstrated that Hh stimulates binding of Evc/Evc2 to Smo depending on phosphorylation of the Smo C-terminal intracellular tail and that the binding is abolished in Kif3a(-/-) cilium-deficient cells. We propose that Hh activates Smo by inducing its phosphorylation, which recruits Evc/Evc2 to activate Gli proteins by antagonizing Sufu in the primary cilia.

  19. Smoothened transduces Hedgehog signal by forming a complex with Evc/Evc2

    Institute of Scientific and Technical Information of China (English)

    Cuiping Yang; Wenlin Chen; Yongbin Chen; Jin Jiang

    2012-01-01

    Hedgehog (Hh) signaling plays pivotal roles in embryonic development and adult tissue homeostasis in species ranging from Drosophila to mammals.The Hh signal is transduced by Smoothened (Smo),a seven-transmembrane protein related to G protein coupled receptors.Despite a conserved mechanism by which Hh activates Smo in Drosophila and mammals,how mammalian Hh signal is transduced from Smo to the Gli transcription factors is poorly understood.Here,we provide evidence that two ciliary proteins,Evc and Evc2,the products of human disease genes responsible for the Ellis-van Creveld syndrome,act downstream of Smo to transduce the Hh signal.We found that loss of Evc/Evc2 does not affect Sonic Hedgehog-induced Smo phosphorylation and ciliary localization but impedes Hh pathway activation mediated by constitutively active forms of Smo.Evc/Evc2 are dispensable for the constitutive Gli activity in Sufu-/- cells,suggesting that Evc/Evc2 act upstream of Sufu to promote Gli activation.Furthermore,we demonstrated that Hh stimulates binding of Evc/Evc2 to Smo depending on phosphorylation of the Smo C-terminal intracellular tail and that the binding is abolished in Kif3a-/- cilium-deficient cells.We propose that Hh activates Smo by inducing its phosphorylation,which recruits Evc/Evc2 to activate Gli proteins by antagonizing Sufu in the primary cilia.

  20. Stress Distribution on the Fe Based Amorphous Toroidal Transducer Core

    Directory of Open Access Journals (Sweden)

    Mustafa Göktepe

    2014-01-01

    Full Text Available The basic principles of sensors are the transmission of energy from one system to another. In general, an electrical signal is produced by the change of a physical property induced by the applied change of a second parameter. In the case of magnetic transducers either the property or the parameter would have a magnetic context. For example, in magnetoelastic toroidal transducers, the induced changes of a physical property, that is, the variation of permeability caused by the applied external force are used to produce a variation in output signal. The linearity, magnitude, sensitivity, and repeatability of the relationship between the output signal of the transducer and the physical property define the quality of the transducer.

  1. Diode-quad bridge for reactive transducers and FM discriminators

    Science.gov (United States)

    Harrison, D. R.; Dimeff, J.

    1972-01-01

    Diode-quad bridge circuit was developed for use with pressure-sensitive capacitive transducers, liquid-level measuring devices, proximity deflection sensors, and inductive displacement sensors. It may also be used as FM discriminator and as universal impedance bridge.

  2. Transducer for Tension Force Measuring of Strip Materials

    Directory of Open Access Journals (Sweden)

    Emad S. Addasi

    2005-01-01

    Full Text Available In winding-up motor drive systems, such as that used in textile industry, it is very important to get a constant tension force for the winding strip material (thread and to reduce its oscillations. This study recommends a transducer with a special design to be used in the mentioned motor drive systems. By using a piston damper, spring, levers, slider and other simple components the suggested sensor (transducer can be used to control the motor speed for getting the required thread tension force. Also the suggested transducer avoids the disadvantage of other used conventional transducer: the parasitic (detrimental oscillations of the thread tension force, which affect the quality of the produced strip material.

  3. Analytical model of a giant magnetostrictive resonance transducer

    Science.gov (United States)

    Sheykholeslami, M.; Hojjat, Y.; Ansari, S.; Cinquemani, S.; Ghodsi, M.

    2016-04-01

    Resonance transducers have been widely developed and studied, as they can be profitably used in many application such as liquid atomizing and sonar technology. The active element of these devices can be a giant magnetostrictive material (GMM) that is known to have significant energy density and good performance at high frequencies. The paper introduces an analytical model of GMM transducers to describe their dynamics in different working conditions and to predict any change in their performance. The knowledge of the transducer behavior, especially in operating conditions different from the ideal ones, is helpful in the design and fabrication of highly efficient devices. This transducer is design to properly work in its second mode of vibration and its working frequency is around 8000 Hz. Most interesting parameters of the device, such as quality factor, bandwidth and output strain are obtained from theoretical analysis.

  4. High-temperature acoustic transducers for use in LMFBR

    International Nuclear Information System (INIS)

    Progress made in the development of piezo-electric and capacitance transducers for acoustic measurements in fast reactors is described. Lithium niobate, because of its high Curie temperature, was the piezo-electric material selected for a study of response to exposure of reactor conditions of temperature, chemistry and irradiation. Potential applications include microphones and pulse-echo ultrasonic transducers. 7Li NbO3 showed greater irradiation tolerance than LiNbO3. Design considerations, details and manufacturing and calibration techniques are described for a range of piezo-electric devices. Several have been tested in air and sodium and have been used in reactor experiments. Capacitance transducers of a type used in sodium rigs and reactors for years are described. Made from irradiation resistant material, they are unaffected by temperature up to 6000C. Smaller transducers are being developed for work in confined spaces. (U.K.)

  5. Piezoelectric and electrostrictive effects in ferroelectret ultrasonic transducers

    Science.gov (United States)

    Döring, Joachim; Bovtun, Viktor; Gaal, Mate; Bartusch, Jürgen; Erhard, Anton; Kreutzbruck, Marc; Yakymenko, Yuriy

    2012-10-01

    Electromechanical response of polypropylene ferroelectret transducers under application of high-voltage pulses was measured by laser Doppler vibrometry and compared with results of ultrasonic through-air transmission between two ferroelectret transducers. The electromechanical response was completely explained by piezoelectric and electrostrictive effects. The electrostrictive effect dominates at high voltages and provides significant enlargement of the transducer constant, up to factor of 2.5. The induced strain of 1.7% was achieved at -2000 V. The nonlinear ultrasonic transmission was shown to be well described by the piezoelectric and electrostrictive response of transmitter, except in the range of high negative exciting voltages where some limitation of the transmitted signal was observed. This limitation seems not to be a fundamental one and does not abolish the advantages of high-voltage excitation of polypropylene ferroelectret transducers.

  6. Wideband Single Crystal Transducer for Bone Characterization Project

    Data.gov (United States)

    National Aeronautics and Space Administration — TRS proposes to develop a simple-to-use, launch capable, ultrasound transducer that is capable of producing the necessary bandwidth to accurately determine in vivo...

  7. Performance Evaluation of Pressure Transducers for Water Impacts

    Science.gov (United States)

    Vassilakos, Gregory J.; Stegall, David E.; Treadway, Sean

    2012-01-01

    The Orion Multi-Purpose Crew Vehicle is being designed for water landings. In order to benchmark the ability of engineering tools to predict water landing loads, test programs are underway for scale model and full-scale water impacts. These test programs are predicated on the reliable measurement of impact pressure histories. Tests have been performed with a variety of pressure transducers from various manufacturers. Both piezoelectric and piezoresistive devices have been tested. Effects such as thermal shock, pinching of the transducer head, and flushness of the transducer mounting have been studied. Data acquisition issues such as sampling rate and anti-aliasing filtering also have been studied. The response of pressure transducers have been compared side-by-side on an impulse test rig and on a 20-inch diameter hemisphere dropped into a pool of water. The results have identified a range of viable configurations for pressure measurement dependent on the objectives of the test program.

  8. Integration of Capacitive Micromachined Ultrasound Transducers to Microfluidic Devices

    KAUST Repository

    Viržonis, Darius

    2013-10-22

    The design and manufacturing flexibility of capacitive micromachined ultrasound transducers (CMUT) makes them attractive option for integration with microfluidic devices both for sensing and fluid manipulation. CMUT concept is introduced here by presentin

  9. Thermal properties photonic crystal fiber transducers with ferromagnetic nanoparticles

    Science.gov (United States)

    Przybysz, N.; Marć, P.; Kisielewska, A.; Jaroszewicz, L. R.

    2015-12-01

    The main aim of the research is to design new types of fiber optic transducers based on filled photonic crystal fibers for sensor applications. In our research we propose to use as a filling material nanoparticles' ferrofluids (Fe3O4 NPs). Optical properties of such transducers are studied by measurements of spectral characteristics' changes when transducers are exposed to temperature and magnetic field changes. From synthesized ferrofluid several mixtures with different NPs' concentrations were prepared. Partially filled commercially available photonic crystal fiber LMA 10 (NKT Photonics) was used to design PCF transducers. Their thermo-optic properties were tested in a temperature chamber. Taking into account magnetic properties of synthetized NPs the patch cords based on a partially filled PM 1550 PCF were measured.

  10. Traceable dynamic calibration of force transducers by primary means

    Science.gov (United States)

    Vlajic, Nicholas; Chijioke, Ako

    2016-08-01

    We describe an apparatus for traceable, dynamic calibration of force transducers using harmonic excitation, and report calibration measurements of force transducers using this apparatus. In this system, the force applied to the transducer is produced by the acceleration of an attached mass, and is determined according to Newton’s second law, F  =  ma. The acceleration is measured by primary means, using laser interferometry. The capabilities of this system are demonstrated by performing dynamic calibrations of two shear-web-type force transducers up to a frequency of 2 kHz, with an expanded uncertainty below 1.2%. We give an account of all significant sources of uncertainty, including a detailed consideration of the effects of dynamic tilting (rocking), which is a leading source of uncertainty in such harmonic force calibration systems.

  11. Engine Oil Condition Monitoring Using High Temperature Integrated Ultrasonic Transducers

    Directory of Open Access Journals (Sweden)

    Jeff Bird

    2011-01-01

    Full Text Available The present work contains two parts. In the first part, high temperature integrated ultrasonic transducers (IUTs made of thick piezoelectric composite films, were coated directly onto lubricant oil supply and sump lines of a modified CF700 turbojet engine. These piezoelectric films were fabricated using a sol-gel spray technology. By operating these IUTs in transmission mode, the amplitude and velocity of transmitted ultrasonic waves across the flow channel of the lubricant oil in supply and sump lines were measured during engine operation. Results have shown that the amplitude of the ultrasonic waves is sensitive to the presence of air bubbles in the oil and that the ultrasound velocity is linearly dependent on oil temperature. In the second part of the work, the sensitivity of ultrasound to engine lubricant oil degradation was investigated by using an ultrasonically equipped and thermally-controlled laboratory testing cell and lubricant oils of different grades. The results have shown that at a given temperature, ultrasound velocity decreases with a decrease in oil viscosity. Based on the results obtained in both parts of the study, ultrasound velocity measurement is proposed for monitoring oil degradation and transient oil temperature variation, whereas ultrasound amplitude measurement is proposed for monitoring air bubble content.

  12. Capacitive Ultrasonic Transducer Development for Acoustic Anemometry on Mars

    Science.gov (United States)

    Leonard-Pugh, Eurion; Wilson, C.; Calcutt, S.; Davis, L.

    2012-10-01

    Previous Mars missions have used either mechanical or thermal anemometry techniques. The moving parts of mechanical anemometers are prone to damage during launch and landing and their inertia makes them unsuited for turbulence studies. Thermal anemometers have been used successfully on Mars but are difficult to calibrate and susceptible to varying ambient temperatures. In ultrasonic anemometry, wind speed and sound speed are calculated from two-way time-of-flight measurements between pairs of transducers; three pairs of transducers are used to return a 3-D wind vector. These high-frequency measurements are highly reliable and immune from drift. Piezo-electric ultrasonic anemometers are widely used on Earth due to their full-range accuracy and high measurement frequency. However these transducers have high acoustic impedances and would not work on Mars. We are developing low-mass capacitive ultrasonic transducers for Mars missions which have significantly lower acoustic impedances and would therefore have a much stronger coupling to the Martian atmosphere. These transducers consist of a metallised polymer film pulled taught against a machined metal backplane. The film is drawn towards the backplane by a DC bias voltage. A varying signal is used on top of the DC bias to oscillate the film; generating acoustic waves. This poster will look at the operation of such sensors and the developments necessary to operate the devices under Martian conditions. Transducer performance is determined primarily by two elements; the front film and the backplane. The sensitivity of the transducer is affected by the thickness of the front film; as well as the diameter, curvature and roughness of the metal backplane. We present data on the performance of the sensors and instrument design considerations including signal shapes and transducer arrangements.

  13. Encapsulation of Capacitive Micromachined Ultrasonic Transducers Using Viscoelastic Polymer

    OpenAIRE

    Lin, Der-Song; Zhuang, Xuefeng; Wong, Serena H.; Kupnik, Mario; Khuri-Yakub, Butrus Thomas

    2010-01-01

    The packaging of a medical imaging or therapeutic ultrasound transducer should provide protective insulation while maintaining high performance. For a capacitive micromachined ultrasonic transducer (CMUT), an ideal encapsulation coating would therefore require a limited and predictable change on the static operation point and the dynamic performance, while insulating the high dc and dc actuation voltages from the environment. To fulfill these requirements, viscoelastic materials, such as poly...

  14. Finite-State Complexity and the Size of Transducers

    Directory of Open Access Journals (Sweden)

    Cristian Calude

    2010-08-01

    Full Text Available Finite-state complexity is a variant of algorithmic information theory obtained by replacing Turing machines with finite transducers. We consider the state-size of transducers needed for minimal descriptions of arbitrary strings and, as our main result, we show that the state-size hierarchy with respect to a standard encoding is infinite. We consider also hierarchies yielded by more general computable encodings.

  15. Complexes between nuclear factor-κB p65 and signal transducer and activator of transcription 3 are key actors in inducing activation-induced cytidine deaminase expression and immunoglobulin A production in CD40L plus interleukin-10-treated human blood B cells.

    Science.gov (United States)

    Lafarge, S; Hamzeh-Cognasse, H; Richard, Y; Pozzetto, B; Cogné, M; Cognasse, F; Garraud, O

    2011-11-01

    The signal transducer and activator of transcription 3 (STAT3) transcription factor pathway plays an important role in many biological phenomena. STAT3 transcription is triggered by cytokine-associated signals. Here, we use isolated human B cells to analyse the role of STAT3 in interleukin (IL)-10 induced terminal B cell differentiation and in immunoglobulin (Ig)A production as a characteristic readout of IL-10 signalling. We identified optimal conditions for inducing in-vitro IgA production by purified blood naive B cells using IL-10 and soluble CD40L. We show that soluble CD40L consistently induces the phosphorylation of nuclear factor (NF)-κB p65 but not of STAT3, while IL-10 induces the phosphorylation of STAT3 but not of NF-κB p65. Interestingly, while soluble CD40L and IL-10 were synergistic in driving the terminal maturation of B cells into IgA-producing plasma cells, they did not co-operate earlier in the pathway with regard to the transcription factors NF-κB p65 or STAT3. Blocking either NF-κB p65 or STAT3 profoundly altered the production of IgA and mRNA for activation-induced cytidine deaminase (AID), an enzyme strictly necessary for Ig heavy chain recombination. Finally, the STAT3 pathway was directly activated by IL-10, while IL-6, the main cytokine otherwise known for activating the STAT3 pathway, did not appear to be involved in IL-10-induced-STAT3 activation. Our results suggest that STAT3 and NF-κB pathways co-operate in IgA production, with soluble CD40L rapidly activating the NF-κB pathway, probably rendering STAT3 probably more reactive to IL-10 signalling. This novel role for STAT3 in B cell development reveals a potential therapeutic or vaccine target for eliciting IgA humoral responses at mucosal interfaces.

  16. Home Automation System Based on Intelligent Transducer Enablers

    Directory of Open Access Journals (Sweden)

    Manuel Suárez-Albela

    2016-09-01

    Full Text Available This paper presents a novel home automation system named HASITE (Home Automation System based on Intelligent Transducer Enablers, which has been specifically designed to identify and configure transducers easily and quickly. These features are especially useful in situations where many transducers are deployed, since their setup becomes a cumbersome task that consumes a significant amount of time and human resources. HASITE simplifies the deployment of a home automation system by using wireless networks and both self-configuration and self-registration protocols. Thanks to the application of these three elements, HASITE is able to add new transducers by just powering them up. According to the tests performed in different realistic scenarios, a transducer is ready to be used in less than 13 s. Moreover, all HASITE functionalities can be accessed through an API, which also allows for the integration of third-party systems. As an example, an Android application based on the API is presented. Remote users can use it to interact with transducers by just using a regular smartphone or a tablet.

  17. Design of a Smart Ultrasonic Transducer for Interconnecting Machine Applications

    Directory of Open Access Journals (Sweden)

    Chang Xu

    2009-06-01

    Full Text Available A high-frequency ultrasonic transducer for copper or gold wire bonding has been designed, analyzed, prototyped and tested. Modeling techniques were used in the design phase and a practical design procedure was established and used. The transducer was decomposed into its elementary components. For each component, an initial design was obtained with simulations using a finite elements model (FEM. Simulated ultrasonic modules were built and characterized experimentally through the Laser Doppler Vibrometer (LDV and electrical resonance spectra. Compared with experimental data, the FEM could be iteratively adjusted and updated. Having achieved a remarkably highly-predictive FEM of the whole transducer, the design parameters could be tuned for the desired applications, then the transducer is fixed on the wire bonder with a complete holder clamping was calculated by the FEM. The approach to mount ultrasonic transducers on wire bonding machines also is of major importance for wire bonding in modern electronic packaging. The presented method can lead to obtaining a nearly complete decoupling clamper design of the transducer to the wire bonder.

  18. Piezoelectric transducer design for a miniaturized injectable acoustic transmitter

    Science.gov (United States)

    Li, H.; Jung, K. W.; Deng, Z. D.

    2015-11-01

    Implantable acoustic transmitters have been used in the last 20 years to track fish movement for fish survival and migration behavior studies. However, the relatively large weights and sizes of commercial transmitters limit the populations of studied fish. The surgical implantation procedures may also affect fish adversely and incur a significant amount of labor. Therefore, a smaller, lighter, and injectable transmitter was needed, and similar or better acoustic performance and service life over those provided by existing commercial transmitters was desired. To develop such a small transmitter, a number of technical challenges, including design optimization of the piezoelectric transducer, needed to be overcome. Our efforts to optimize the transducer focused on improving the average source level in the 180° range in which the signal was not blocked by the transmitter body. We found that a novel off-center tube transducer improved the average source level by 1.5 dB. An acoustic reflector attached to the back of the transducer also improved the source level by 1.3 dB. We found that too small a gap between the transducer and the component placed behind it resulted in distortion of the beam pattern. Lastly, a tuning inductor in series with the transducer was used to help optimize the source level. The findings and techniques developed in this work contributed to the successful development and implementation of a new injectable transmitter.

  19. Volumetric loss quantification using ultrasonic inductively coupled transducers

    Science.gov (United States)

    Gong, Peng; Hay, Thomas R.; Greve, David W.; Oppenheim, Irving J.

    2015-03-01

    The pulse-echo method is widely used for plate and pipe thickness measurement. However, the pulse echo method does not work well for detecting localized volumetric loss in thick-wall tubes, as created by erosion damage, when the morphology of volumetric loss is irregular and can reflect ultrasonic pulses away from the transducer, making it difficult to detect an echo. In this paper, we propose a novel method using an inductively coupled transducer to generate longitudinal waves propagating in a thick-wall aluminum tube for the volumetric loss quantification. In the experiment, longitudinal waves exhibit diffraction effects during the propagation which can be explained by the Huygens-Fresnel principle. The diffractive waves are also shown to be significantly delayed by the machined volumetric loss on the inside surface of the thick-wall aluminum tube. It is also shown that the inductively coupled transducers can generate and receive similar ultrasonic waves to those from wired transducers, and the inductively coupled transducers perform as well as the wired transducers in the volumetric loss quantification when other conditions are the same.

  20. Characterization of noncontact piezoelectric transducer with conically shaped piezoelement

    Science.gov (United States)

    Williams, James H., Jr.; Ochi, Simeon C. U.

    1988-01-01

    The characterization of a dynamic surface displacement transducer (IQI Model 501) by a noncontact method is presented. The transducer is designed for ultrasonic as well as acoustic emission measurements and, according to the manufacturer, its characteristic features include a flat frequency response range which is from 50 to 1000 kHz and a quality factor Q of less than unity. The characterization is based on the behavior of the transducer as a receiver and involves exciting the transducer directly by transient pulse input stress signals of quasi-electrostatic origin and observing its response in a digital storage oscilloscope. Theoretical models for studying the response of the transducer to pulse input stress signals and for generating pulse stress signals are presented. The characteristic features of the transducer which include the central frequency f sub o, quality factor Q, and flat frequency response range are obtained by this noncontact characterization technique and they compare favorably with those obtained by a tone burst method which are also presented.

  1. Experimental Evaluation of Three Designs of Electrodynamic Flexural Transducers

    Science.gov (United States)

    Eriksson, Tobias J. R.; Laws, Michael; Kang, Lei; Fan, Yichao; Ramadas, Sivaram N.; Dixon, Steve

    2016-01-01

    Three designs for electrodynamic flexural transducers (EDFT) for air-coupled ultrasonics are presented and compared. An all-metal housing was used for robustness, which makes the designs more suitable for industrial applications. The housing is designed such that there is a thin metal plate at the front, with a fundamental flexural vibration mode at ∼50 kHz. By using a flexural resonance mode, good coupling to the load medium was achieved without the use of matching layers. The front radiating plate is actuated electrodynamically by a spiral coil inside the transducer, which produces an induced magnetic field when an AC current is applied to it. The transducers operate without the use of piezoelectric materials, which can simplify manufacturing and prolong the lifetime of the transducers, as well as open up possibilities for high-temperature applications. The results show that different designs perform best for the generation and reception of ultrasound. All three designs produced large acoustic pressure outputs, with a recorded sound pressure level (SPL) above 120 dB at a 40 cm distance from the highest output transducer. The sensitivity of the transducers was low, however, with single shot signal-to-noise ratio (SNR)≃15 dB in transmit–receive mode, with transmitter and receiver 40 cm apart. PMID:27571075

  2. Piezoelectric films for high frequency ultrasonic transducers in biomedical applications.

    Science.gov (United States)

    Zhou, Qifa; Lau, Sienting; Wu, Dawei; Shung, K Kirk

    2011-02-01

    Piezoelectric films have recently attracted considerable attention in the development of various sensor and actuator devices such as nonvolatile memories, tunable microwave circuits and ultrasound transducers. In this paper, an overview of the state of art in piezoelectric films for high frequency transducer applications is presented. Firstly, the basic principles of piezoelectric materials and design considerations for ultrasound transducers will be introduced. Following the review, the current status of the piezoelectric films and recent progress in the development of high frequency ultrasonic transducers will be discussed. Then details for preparation and structure of the materials derived from piezoelectric thick film technologies will be described. Both chemical and physical methods are included in the discussion, namely, the sol-gel approach, aerosol technology and hydrothermal method. The electric and piezoelectric properties of the piezoelectric films, which are very important for transducer applications, such as permittivity and electromechanical coupling factor, are also addressed. Finally, the recent developments in the high frequency transducers and arrays with piezoelectric ZnO and PZT thick film using MEMS technology are presented. In addition, current problems and further direction of the piezoelectric films for very high frequency ultrasound application (up to GHz) are also discussed.

  3. Software for Correcting the Dynamic Error of Force Transducers

    Directory of Open Access Journals (Sweden)

    Naoki Miyashita

    2014-07-01

    Full Text Available Software which corrects the dynamic error of force transducers in impact force measurements using their own output signal has been developed. The software corrects the output waveform of the transducers using the output waveform itself, estimates its uncertainty and displays the results. In the experiment, the dynamic error of three transducers of the same model are evaluated using the Levitation Mass Method (LMM, in which the impact forces applied to the transducers are accurately determined as the inertial force of the moving part of the aerostatic linear bearing. The parameters for correcting the dynamic error are determined from the results of one set of impact measurements of one transducer. Then, the validity of the obtained parameters is evaluated using the results of the other sets of measurements of all the three transducers. The uncertainties in the uncorrected force and those in the corrected force are also estimated. If manufacturers determine the correction parameters for each model using the proposed method, and provide the software with the parameters corresponding to each model, then users can obtain the waveform corrected against dynamic error and its uncertainty. The present status and the future prospects of the developed software are discussed in this paper.

  4. Experimental Evaluation of Three Designs of Electrodynamic Flexural Transducers.

    Science.gov (United States)

    Eriksson, Tobias J R; Laws, Michael; Kang, Lei; Fan, Yichao; Ramadas, Sivaram N; Dixon, Steve

    2016-01-01

    Three designs for electrodynamic flexural transducers (EDFT) for air-coupled ultrasonics are presented and compared. An all-metal housing was used for robustness, which makes the designs more suitable for industrial applications. The housing is designed such that there is a thin metal plate at the front, with a fundamental flexural vibration mode at ∼50 kHz. By using a flexural resonance mode, good coupling to the load medium was achieved without the use of matching layers. The front radiating plate is actuated electrodynamically by a spiral coil inside the transducer, which produces an induced magnetic field when an AC current is applied to it. The transducers operate without the use of piezoelectric materials, which can simplify manufacturing and prolong the lifetime of the transducers, as well as open up possibilities for high-temperature applications. The results show that different designs perform best for the generation and reception of ultrasound. All three designs produced large acoustic pressure outputs, with a recorded sound pressure level (SPL) above 120 dB at a 40 cm distance from the highest output transducer. The sensitivity of the transducers was low, however, with single shot signal-to-noise ratio ( SNR ) ≃ 15 dB in transmit-receive mode, with transmitter and receiver 40 cm apart. PMID:27571075

  5. Focusing of ferroelectret air-coupled ultrasound transducers

    Science.gov (United States)

    Gaal, Mate; Bartusch, Jürgen; Dohse, Elmar; Schadow, Florian; Köppe, Enrico

    2016-02-01

    Air-coupled ultrasound has been applied increasingly as a non-destructive testing method for lightweight construction in recent years. It is particularly appropriate for composite materials being used in automotive and aviation industry. Air-coupled ultrasound transducers mostly consist of piezoelectric materials and matching layers. However, their fabrication is challenging and their signal-to-noise ratio often not sufficient for many testing requirements. To enhance the efficiency, air-coupled ultrasound transducers made of cellular polypropylene have been developed. Because of its small density and sound velocity, this piezoelectric ferroelectret matches the small acoustic impedance of air much better than matching layers applied in conventional transducers. In our contribution, we present two different methods of spherical focusing of ferroelectret transducers for the further enhancement of their performance in NDT applications. Measurements on carbon-fiber-reinforced polymer (CFRP) samples and on metal adhesive joints performed with commercially available focused air-coupled ultrasound transducers are compared to measurements executed with self-developed focused ferroelectret transducers.

  6. Experimental Analysis of Bisbenzocyclobutene Bonded Capacitive Micromachined Ultrasonic Transducers.

    Science.gov (United States)

    Manwar, Rayyan; Chowdhury, Sazzadur

    2016-01-01

    Experimental measurement results of a 1.75 mm × 1.75 mm footprint area Capacitive Micromachined Ultrasonic Transducer (CMUT) planar array fabricated using a bisbenzocyclobutene (BCB)-based adhesive wafer bonding technique has been presented. The array consists of 40 × 40 square diaphragm CMUT cells with a cavity thickness of 900 nm and supported by 10 µm wide dielectric spacers patterned on a thin layer of BCB. A 150 µm wide one µm thick gold strip has been used as the contact pad for gold wire bonding. The measured resonant frequency of 19.3 MHz using a Polytec™ laser Doppler vibrometer (Polytec™ MSA-500) is in excellent agreement with the 3-D FEA simulation result using IntelliSuite™. An Agilent ENA5061B vector network analyzer (VNA) has been used for impedance measurement and the resonance and anti-resonance values from the imaginary impedance curve were used to determine the electromechanical coupling co-efficient. The measured coupling coefficient of 0.294 at 20 V DC bias exhibits 40% higher transduction efficiency as compared to a measured value published elsewhere for a silicon nitride based CMUT. A white light interferometry method was used to measure the diaphragm deflection profiles at different DC bias. The diaphragm center velocity was measured for different sub-resonant frequencies using a Polytec™ laser Doppler vibrometer that confirms vibration of the diaphragm at different excitation frequencies and bias voltages. Transmit and receive operations of CMUT cells were characterized using a pitch-catch method and a -6 dB fractional bandwidth of 23% was extracted from the received signal in frequency domain. From the measurement, it appears that BCB-based CMUTs offer superior transduction efficiency as compared to silicon nitride or silicon dioxide insulator-based CMUTs, and provide a very uniform deflection profile thus making them a suitable candidate to fabricate highly energy efficient CMUTs. PMID:27347955

  7. Ultrasonic flowmeters: temperature gradients and transducer geometry effects.

    Science.gov (United States)

    Willatzen, M

    2003-03-01

    Ultrasonic flowmeter performance is addressed for the case of cylindrically shaped flowmeters employing two reciprocal ultrasonic transducers A and B so as to measure time-of-flight differences between signals transmitted from transducer A towards B followed by an equivalent signal transmitted from transducer B towards A. In the case where a liquid flows through the flowmeter's measuring section ("spoolpiece"), the arrival times of the two signals differ by an amount related to the flow passing between the two transducers. Firstly, a detailed study of flow measurement errors with mean flow in the laminar flow regime is carried out as a function of the mode index and the transducer diameter/cylinder diameter ratio in the case where no temperature gradients are present in the flowmeter sensor. It is shown that all modes except the fundamental mode overestimate the mean flow by a factor of 33.33% while excitation of the fundamental mode solely give error-free measurements. The immediate consequences are that the flowmeter error decreases as the transducer diameter/cylinder diameter ratio approaches 1 from 0 reflecting the fact that the excitation level of the fundamental mode increases from almost 0 to 1 as this ratio approaches 1 from 0. Secondly, the effect on flowmeter performance due to flow-induced temperature gradients is examined. It is shown that the presence of temperature gradients leads to flowmeter errors at the higher-flow values even in the case where the fundamental mode is the only mode excited. It is also deduced that flowmeter errors in general depend on the distance between transducers A and B whether temperature gradients exist or not. This conclusion is not reflected in the usual definition of flowmeter errors given by the so-called mode-dependent deviation of measurement introduced in earlier works. PMID:12565074

  8. Design of a saturated analogue and digital current transducer

    International Nuclear Information System (INIS)

    This project describes the development of a new analogue and digital current transducer, providing a range of new theoretical design methods for these novel devices. The main control feature is the limit cycling operation, and the novel use of the embedded sigma-delta modulator sensor structure to derive a low component count digital sensor. The research programme was initiated into the design, development and evaluation of a novel non-Hall sensing analogue and digital current transducer. These transducers are used for measurement of high currents in power systems applications. The investigation is concerned with a new design which uses a magnetic ferrite core without an air gap for current measurement. The motivation for this work was to design a new control circuit which provides a low component count, and utilises the non-linear properties of the magnetic ferrite core to transmit direct current. The use of a limit cycle control circuit was believed to be particularly suitable for the analogue and digital transducers, for two main reasons: the low component count, and the output signal is directly digital. In line with the motivations outlined above, the outcome of the research has witnessed the design, development and evaluation of a practically realisable analogue and digital current transducer. The design procedure, which is documented in this thesis, is considered to be a major contribution to the field of transducers design and development using a control systems approach. Mathematical models for both analogue and digital transducers were developed and the resulting model based predictions were found to be in good agreement with measured results. Simplification of the new model sensing device was achieved by approximating the non-linear ferrite core using FFT analysis. This is also considered to be a significant contribution. The development analogue and digital current censors employed a sampled data control systems design and utilised limit cycling

  9. Transducing Hedgehog: the story so far.

    OpenAIRE

    Ingham, P W

    1998-01-01

    The secreted proteins of the Hedgehog family have been implicated in many different processes in vertebrate development including cartilage differentiation, myotome and sclerotome specification, hair follicle development, limb morphogenesis and the specification of different neuronal cell types. In addition, the aberrant activation of the Hedgehog pathway has been identified as the likely cause of a number of tumours in humans including basal cell carcinomas (BCCs) and primitive neurectoderma...

  10. 1-3 Piezocomposite transducers for AUV applications

    Science.gov (United States)

    Pazol, Brian; Lannaman, Ken; Doust, Barry

    2001-05-01

    Sonar systems on board AUVs present interesting challenges to the transducer designer because of their small size, low weight requirements, and limited available power. 1-3 piezocomposite transducers offer many performance characteristics which make them ideal for deployment in AUVs. Piezocomposite transducers are light weight, have broad bandwidth, have high efficiency, and can be conformed to fit the curvature of the vehicle. The broad bandwidths and low sidelobes made possible by piezocomposites result in sharper images with less distortion. The piezocomposite material is mechanically robust and can survive the rigors of normal operations as well as AUV deployment and retrieval. In addition, the conformal configuration substantially reduces hydrodynamic drag. As a conformal array, there is nothing to get knocked off during deployment and retrieval operations, or entangled with natural or man-made objects suspended in the water column. This contributes directly to improving the operational endurance of the AUV system, thereby enhancing overall system utility. MSI has produced and tested a variety of piezocomposite transducers for use in obstacle avoidance, mine hunting, and acoustic communications. An overview of piezocomposites and recent results of piezocomposite transducers will be presented.

  11. Design of Capacitance to Voltage Converter for Capacitive Sensor Transducer

    Directory of Open Access Journals (Sweden)

    A. H.M.Z. Alam

    2010-01-01

    Full Text Available Problem statement: The design of Capacitance to Voltage Converter (CVC for capacitive sensor transducer was presented. The proposed design will reduce the size, power consumption and supply voltage of the circuit and can be used in high frequency band transducer. Approach: The design was implemented using the Operational amplifier (Op amp and capacitive network. The circuit was simulated using the PSPICE model parameters based on standard 0.13 μm CMOS process. Results: The design was able to measure a wide range of capacitance variations for the capacitive transducer. The performance analysis of the design showed desirable performance parameters in terms of response, low power consumption and a linear output voltage within the wide range of capacitive transducer capacitance variation for the power supply voltage of 1.2 V was achieved. Conclusion/Recommendations: The output voltage of the circuit varied linearly with the variation of capacitive transducer capacitance variation. The improved converter was compact and robust for integration into capacitive measuring systems and suitable for use in environment that making use of higher frequency band.

  12. Piezoelectric Polymer Ultrasound Transducers and Its Biomedical Applications

    Energy Technology Data Exchange (ETDEWEB)

    Ha, Kang Lyeol; Cao, Yanggang [Department of Physics, Pukyong National University, Busan (Korea, Republic of)

    2012-10-15

    PVDF(poly vinylidene fluoride) and P(VDF-TrFE)(poly vinylidene fluoride-tetrafluoroethylene) are the typical piezoelectric polymers with unique properties. Even they are inferior to conventional piezoelectric ceramics PZT in electromechanical conversion efficiency and interior loss, though they are superior in receiving sensitivity and frequency bandwidth. Their acoustic impedances are relatively close to water or biological tissue and it is easier to make thin film than other piezoelectric materials. Furthermore, the film is so flexible that it is easy to attach on a complex surface. Those properties are suitable for the ultrasound transducers which are useful for medical and biological application, so that various types of polymer transducers have been developed. In this paper, several important considerations for design and fabrication of piezoelectric polymer transducers were described and their effect on the transducer performance were demonstrated through the KLM model analysis. Then, it was briefly reviewed about the structures of the polymer transducers developed for obtaining images as well as the characteristics of the images in several important medical and biological application fields.

  13. Long-Term Stability of the NIST Conical Reference Transducer.

    Science.gov (United States)

    Fick, Steven E; Proctor, Thomas M

    2011-01-01

    The National Institute of Standards and Technology (NIST) Conical Reference Transducer (CRT) is designed for purposes requiring frequency response characteristics much more uniform than those attainable with ultrasonic transducers conventionally used for acoustic emission (AE) nondestructive testing. The high performance of the CRT results from the use of design elements radically different from those of conventional transducers. The CRT was offered for sale for 15 years (1985 to 2000). Each CRT was furnished with data which expressed, as a function of frequency, the transducer sensitivity in volts per micrometer of normal displacement on the test block. Of the 22 transducers constructed, eight were reserved for long term research and were stored undisturbed in a laboratory with well controlled temperature and humidity. In 2009, the sensitivities of these eight units were redetermined. The 2009 data have been compared with data from similar tests conducted in 1985. The results of this comparison verify the claim "Results of tests of the long term stability of CRT characteristics indicate that, if proper care is taken, tens of years of service can reasonably be expected." made in the CRT specifications document furnished to prospective customers. PMID:26989602

  14. Characterization of Transducers and Resonators under High Drive Levels

    Science.gov (United States)

    Sherrit, Stewart; Bao, X.; Sigel, D. A.; Gradziel, M. J.; Askins, S. A.; Dolgin, B. P.; Bar-Cohen, Y.

    2001-01-01

    In many applications, piezoelectric transducers are driven at AC voltage levels well beyond the level for which the material was nominally characterized. In this paper we describe an experimental setup that allows for the determination of the main transducer or resonator properties under large AC drive. A sinusoidal voltage from a waveform generator is amplified and applied across the transducer/resonator in series with a known high power resistor. The amplitude of applied voltage and the amplitude and the relative phase of the current through the resistor are monitored on a digital scope. The frequency of the applied signal is swept through resonance and the voltage/current signals are recorded. After corrections for the series resistance and parasitic elements the technique allows for the determination of the complex impedance spectra of the sample as a function of frequency. In addition, access to the current signal allows for the direct investigation of non-linear effects through the application of Fourier transform techniques on the current signal. Our results indicate that care is required when interpreting impedance data at high drive level due to the frequency dependence of the dissipated power. Although the transducer/resonator at a single frequency and after many cycles may reach thermal equilibrium, the spectra as a whole cannot be considered an isothermal measurement due to the temperature change with frequency. Methods to correct for this effect will be discussed. Results determined from resonators of both soft and hard PZT and a ultrasonic horn transducer are presented.

  15. Ultrasonic array of thick film transducers for biological tissue characterization.

    Science.gov (United States)

    Gwirc, Sergio N; Negreira, Carlos A; Marino, Nestor R

    2010-01-01

    The initial motivation for this work was to accomplish an easy way to manufacture different geometries of ultrasonic transducers and arrays using a PZT powder, combined with a standard process to have repetitive series of them. The piezoelectric thick film was obtained using a PZT paste and applying it by screen printing on an alumina substrate. Then, the film was drying and sintered with a temperature-time profile determined by the paste characteristics. Each transducer is composed by three layers, one by PZT and two acting as electrodes. The active element of the paste is a PZT powder which is dispersed in a commercial vehicle to obtain rheological properties suitable for use the screen printing process. The connection between PZT particles is improved by adding a lead borosilicate frit glass that also helps to attach the film to the substrate due to the relatively low temperature of sintered that has been used in this process. The PZT film has low density that is generated by internal porosity, so its acoustic impedance is lower than for a bulk ceramic transducer and so is well adapted to testing human tissues. At the same time the thick film technology is well suited to make medium size transducers and also arrays performed with tiny ultrasonic transducers. PMID:21097177

  16. Delimitation of the lung region with distributed ultrasound transducers

    Science.gov (United States)

    Cardona Cárdenas, Diego Armando; Furuie, Sérgio Shiguemi

    2016-04-01

    One technique used to infer and monitor patient's respiratory conditions is the electrical impedance tomography (EIT). This provides images with information about lung function. The EIT image contrast is dependent on the variation of electrical impedance, therefore, this image does not provide anatomical details in border regions of several organs. To contribute to a clinical solution, we propose a new method to delimit regions of interest such as the pulmonary region and to improve the reconstruction quality of the EIT. Using a Matlab Toolbox k-wave, the ultrasound propagation phenomenon in homogeneous medium without patient (Reference) and with thoracic models were simulated, separately via a set of several ultrasound transducers distributed around the chest. After pulse emission by a transducer (TR), all received signals were compared considering the two sets of signals. If the energy relation between parts of the signals does not exceed an empirical threshold (30% in this study), a partial mask is generated between the transmitter and the receptor. This process was repeated until all 128 transducers are considered as TR-emitters. The 128 transducers (150kHz) are uniformly distributed. The evaluation was made by visually comparing the resulting images with the respective simulated object. A simple approach was presented to delimit high contrast organs with ultrasound transducers distributed around the patient. This approach allows other lower contrast objects to become invisible by varying the threshold limit. The investigation, based on numerical simulations of ultrasonic propagation, has shown promising results in the delimitation of the pulmonary region.

  17. Investigation of bulk acoustic microwaves excited by an interdigital transducer

    Directory of Open Access Journals (Sweden)

    Reshotka O. G.

    2015-12-01

    Full Text Available Excitation of bulk and surface acoustic waves with the interdigital transducer (IDT, which is deposited on the surface of piezoelectric crystal, is widely used in the development of devices in acoustoelectronics and in the design of the microwave acousto-optic deflectors. Excitation of bulk acoustic waves by IDT in the devices on surface acoustic waves leads to the appearance of spurious signals. At the same time excitation of bulk acoustic waves with IDT from the surface of lithium niobate crystals allows creating high frequency acousto-optic deflectors, which makes possible to significantly simplify the technology of their production. Therefore, significant attention is paid to the task of excitation and distribution of bulk acoustic waves with IDT including recent times by the method of simulation of their excitation and distribution. The obtained theoretical results require experimental verification. This paper documents the visualization of acoustic beams excited with IDT from the XY-surface of lithium niobate crystals. The Bragg cells with LiNbO3 crystals coated with IDT with a different period of electrodes were manufactured for the experimental research of excitation and distribution of bulk acoustic waves. Visualization results have shown that the acoustic waves excited with IDT distribute in both the Fresnel zone and the Fraunhofer zone. The length of these zones is caused by individual elementary emitters of which consists the IDT (by their size. At the same time the far zone for IDT is located at distances much greater than the actual size of the LiNbO3 crystals. This peculiarity is not always taken into account when calculating diffraction. The achieved results can be used to design high-frequency acousto-optic devices, as well as in the development of devices based on surface acoustic waves.

  18. Reducing the Effect of Transducer Mount Induced Noise on Aeroacoustic Wind Tunnel Testing Data with a New Transducer Mount Design

    Science.gov (United States)

    Herron, A. J.; Reed, D. K.; Nance, D. K.

    2015-01-01

    Characterization of launch vehicle unsteady aerodynamics is a field best studied through experimentation, which is often carried out in the form of large scale wind tunnel testing. Measurement of the fluctuating pressures induced by the boundary layer noise is customarily made with miniature pressure transducers installed into a model of the vehicle of interest. Literature shows that noise level increases between two to five decibels (dB referenced to 20 micropascal) can be induced when the transducer surface is not mounted perfectly flush with the model outer surface. To reduce this artificially induced noise, special transducer holders have been used for aeroacoustic wind tunnel testing by NASA. This holder is a sleeve into which the transducer fits, with a cap that allows it to be mounted in a recessed hole in the model. A single hole in the holder allows the transport of the tunnel medium so the transducer can discriminate the fluctuating pressure due to the turbulent boundary layer noise. The holder is first dry fitted into the model and any difference in height between the holder and the model surface can be sanded flush. The holder is then removed from the model, the transducer glued inside the holder, and the holder replaced in the model, secured also with glue, thus eliminating the problem of noise level increases due to lack of flushness. In order to work with this holder design, special transducers have been ordered with their standard screen removed and the diaphragm moved as close to the top of the casing as possible to minimize any cavity volume. Although this greatly reduces induced noise due to the transducers being out of flush, the holders can also induce a cavity resonance that is usually at a very high frequency. This noise is termed transducer mount induced noise (XMIN). The peak of the mode can vary with the cavity depth, boundary layer noise that can excite the mode, tunnel flow medium, and the build of the transducers. Because the boundary

  19. INFLUENCE OF PIEZOELECTRIC TRANSDUCER TO GLASS FIBER REINFORCED COMPOSITE STIFFNESS

    Directory of Open Access Journals (Sweden)

    Witold Rządkowski

    2015-08-01

    Full Text Available The main goal was to determine if transducers based on piezoelectric materials are suitable for strain calculations in thin GFRP specimens. Numerous experimental studies, both physical and numerical, performed by the authors, have shown that there is a huge influence of bonded piezoelectric transducer on the overall stiffness of the measured object. The paper presents tensile test performed on strength machine with Digital Image Correlation strain and deflection observations. Test were compared with FEM models for detailed investigation. The main conclusion is piezoelectric transducers has huge influence on local stiffness of measured object. That is critical especially when they are used as strain sensors, when presence of sensor is influencing to measured results.

  20. Energy harvesting with a slotted-cymbal transducer

    Institute of Scientific and Technical Information of China (English)

    Jiang-bo YUAN; Xiao-biao SHAN; Tao XIE; Wei-shan CHEN

    2009-01-01

    A cymbal transducer is made up of a piezoceramic disk sandwiched between two dome-shaped metal endcaps. High circumferential stresses caused by flexural motion of the metal endcaps can induce the loss of mechanical input energy. Finite element analysis shows that the radial slots fabricated in metal endcaps can release the circumferential stresses, and reduce the loss of mechanical input energy that could be converted into electrical energy. In this letter, the performance of a slotted-cymbal transducer in energy harvesting was tested. The results show that the output voltage and power of the cymbal are improved. A maximum output power of around 16 mW could be harvested from a cymbal with 18 cone radial slots across a 500kΩ resistive load, which is approximately 0.6 times more than that of the original cymbal transducer.

  1. Ultrasonic waveguide transducer for high temperature testing of ceramic honeycomb

    Science.gov (United States)

    Wang, N.; An, C. P.; Nickerson, S. T.; Gunasekaran, N.; Shi, Z.

    2013-01-01

    This paper describes the development of a practical ultrasonic waveguide transducer designed for in situ material property characterization of ceramic honeycomb at high temperatures (>1200°C) and under fast thermal cycles (>1000°C/min). The low thermal conductivity MACOR waveguide allows the use of conventional transducer (max temp. 50°C) at one end and guides ultrasonic waves into the high temperature region where the characterization is carried out. The impact of time, temperature, and heating/cooling rates on the material behavior was studied. It was demonstrated that the same transducer could also be used for in-situ crack detection during the thermal shock testing of ceramic honeycomb.

  2. Improved capacitive stress transducers for high-field superconducting magnets

    Science.gov (United States)

    Benson, Christopher Pete; Holik, Eddie Frank, III; Jaisle, Andrew; McInturff, A.; McIntyre, P.

    2012-06-01

    High-field (12-18 Tesla) superconducting magnets are required to enable an increase in the energy of future colliders. Such field strength requires the use of Nb3Sn superconductor, which has limited tolerance for compressive and shear strain. A strategy for stress management has been developed at Texas A&M University and is being implemented in TAMU3, a short-model 14 Tesla stress-managed Nb3Sn block dipole. The strategy includes the use of laminar capacitive stress transducers to monitor the stresses within the coil package. We have developed fabrication techniques and fixtures, which improve the reproducibility of the transducer response both at room temperature and during cryogenic operation. This is a report of the status of transducer development.

  3. Apparent transducer non-reciprocity in an ultrasonic flow meter.

    Science.gov (United States)

    van Deventer, Jan; Delsing, Jerker

    2002-05-01

    This paper investigates the effects of non-identical ultrasonic transducers on reciprocity and zero-flow calibration in transit time flow meters. According to the theorem of reciprocity, there should not be any difference between the up- and downstream acoustic times of flight in a zero-flow situation. This would thus eliminate zero-flow estimation drifts. The flow meter is modeled as a one dimensional system with equivalent electrical circuits and simulated with simulation program with integrated circuits emphasis. The work shows that variations between the two transducers cause false estimates of flow and indicate which parameters have the largest influence. It indicates that reciprocity holds only for identical transducers. PMID:12159974

  4. Novice performance of ultrasound-guided needle advancement: standard 38-mm transducer vs 25-mm hockey stick transducer.

    Science.gov (United States)

    Davies, T; Townsley, P; Jlala, H; Dowling, M; Bedforth, N; Hardman, J G; McCahon, R A

    2012-08-01

    The optimal method to develop expertise in ultrasound-guided regional anaesthesia is unknown. Studies of laryngoscopic expertise in novices demonstrate that the choice of laryngoscope affects performance. In this study, we aimed to compare the effect of two different linear array transducers (38-mm standard vs 25-mm hockey stick) on novice performance of ultrasound-guided needle advancement. Following randomisation, participants watched a video model of expert performance of ultrasound-guided needle advancement. Recruits performed the modelled task on a turkey breast model. The median (IQR [range]) composite error score was statistically significantly larger for participants in the hockey stick transducer group compared with the standard transducer group; 10.0 (7.3-14.3 [2.5-29.0]) vs 7.5 (4.5-10.0 [2.0-28.0]) respectively, (p = 0.01). This study has demonstrated that performance of ultrasound-guided needle advancement by novice operators after simple video instruction is better (as assessed using a composite error score) with a standard 38-mm transducer than with a 25-mm hockey stick transducer. PMID:22506607

  5. High intensity ultrasound transducer used in gene transfection

    Science.gov (United States)

    Morrison, Kyle P.; Keilman, George W.; Noble, Misty L.; Brayman, Andrew A.; Miao, Carol H.

    2012-11-01

    This paper describes a novel therapeutic high intensity non-focused ultrasound (HIU) transducer designed with uniform pressure distribution to aid in accelerated gene transfer in large animal liver tissues in vivo. The underlying HIU transducer was used to initiate homogeneous cavitation throughout the tissue while delivering up to 2.7 MPa at 1.1 MHz across its radiating surface. The HIU transducer was built into a 6 cm diameter x 1.3 cm tall housing ergonomically designed to avoid collateral damage to the surrounding anatomy during dynamic motion. The ultrasound (US) radiation was applied in a 'paintbrush-like' manner to the surface of the liver. The layers and geometry of the transducer were carefully selected to maximize the active diameter (5.74 cm), maximize the electrical to acoustic conversion efficiency (85%) to achieve 2.7 MPa of peak negative pressure, maximize the frequency operating band at the fundamental resonance to within a power transfer delta of 1 dB, and reduce the pressure delta to within 2 dB across the radiating surface. For maximum peak voltage into the transducer, a high performance piezoceramic was chosen and a DC bias circuit was built integral to the system. An apodized two element annular pattern was made from a single piezoceramic element, resulting in significant pressure uniformity enhancement. In addition to using apodization for pressure uniformity, a proprietary multi-layered structure was used to improve efficiency while sustaining an operating band from 900 kHz to 1.3 MHz. The resultant operating band allowed for dithering techniques using frequency modulation. The underlying HIU transducer for use in large animals enhances gene expression up to 6300-fold.

  6. Transducers for Sound and Vibration - FEM Based Design

    DEFF Research Database (Denmark)

    Liu, Bin

    2001-01-01

    for methods that can reduce the design time consumption and the number of itterations. The present work proposes to use finite element based programs for simulating the behaviour of a transducer with a given set of specifications. A simulation program for accelerometers was developed and has been tested......Design of transducers for measurement of vibration (piezoelectric accelerometers) and sound (condenser microphones) is a very labour intensive work. The design work is mostly based on experience and on simple analogies to electrical circuit design. Often a time consuming itterative loop is used...

  7. Thermal energy harvesters with piezoelectric or electrostatic transducer

    Science.gov (United States)

    Prokaryn, Piotr; Domański, Krzysztof; Marchewka, Michał; Tomaszewski, Daniel; Grabiec, Piotr; Puscasu, Onoriu; Monfray, Stéphane; Skotnicki, Thomas

    2014-08-01

    This paper describes the idea of the energy harvester which converts thermal gradient present in environment into electricity. Two kinds of such devices are proposed and their prototypes are shown and discussed. The main parts of harvesters are bimetallic spring, piezoelectric transducer or electrostatic transducer with electret. The applied piezomembrane was commercial available product but electrets was made by authors. In the paper a fabrication procedure of electrets formed by the corona discharge process is described. Devices were compared in terms of generated power, charging current, and the voltage across a storage capacitor.

  8. Qualification of indigenously developed sodium compatible ultrasonic transducers for PFBR

    International Nuclear Information System (INIS)

    PFBR core is immersed in a pool of optically opaque liquid sodium at high temperature. Ultrasonic technique is utilized to view the components immersed in sodium. An under sodium ultrasonic scanner (USUSS) with indigenously developed sodium compatible transducers are used to scan the core plenum before every fuel handling operation, when the temperature of sodium is around 180℃. There are two different types of transducers, one for the measurement of bowing and the other is used for the protrusion measurement of any fuel sub assembly (FSA). (author)

  9. Wideband Single-Crystal Transducer for Bone Characterization

    Science.gov (United States)

    Liang, Yu; Snook, Kevin

    2012-01-01

    The microgravity conditions of space travel result in unique physiological demands on the human body. In particular, the absence of the continual mechanical stresses on the skeletal system that are present on Earth cause the bones to decalcify. Trabecular structure decreases in thickness and increases in spacing, resulting in decreased bone strength and increased risk of injury. Thus, monitoring bone health is a high priority for long-term space travel. A single probe covering all frequency bands of interest would be ideal for such measurements, and this would also minimize storage space and eliminate the complexity of integrating multiple probes. This invention is an ultrasound transducer for the structural characterization of bone. Such characterization measures features of reflected and transmitted ultrasound signals, and correlates these signals with bone structure metrics such as bone mineral density, trabecular spacing, and thickness, etc. The techniques used to determine these various metrics require measurements over a broad range of ultrasound frequencies, and therefore, complete characterization requires the use of several narrowband transducers. This is a single transducer capable of making these measurements in all the required frequency bands. The device achieves this capability through a unique combination of a broadband piezoelectric material; a design incorporating multiple resonator sizes with distinct, overlapping frequency spectra; and a micromachining process for producing the multiple-resonator pattern with common electrode surfaces between the resonators. This device consists of a pattern of resonator bars with common electrodes that is wrapped around a central mandrel such that the radiating faces of the resonators are coplanar and can be simultaneously applied to the sample to be measured. The device operates as both a source and receiver of acoustic energy. It is operated by connection to an electronic system capable of both providing an

  10. Simulating Capacitive Micromachined Ultrasonic Transducers (CMUTs) using Field II

    DEFF Research Database (Denmark)

    Bæk, David; Oralkan, Omer; Kupnik, Mario;

    2010-01-01

    Field II has been a recognized simulation tool for piezoceramic medical transducer arrays for more than a decade. The program has its strength in doing fast computations of the spatial impulse response (SIR) from array elements by dividing the elements into smaller mathematical elements (ME)s from...... which it calculates the SIR responses. The program features predefined models for classical transducer geometries, but currently none for the fast advancing CMUTs. This work addresses the assumptions required for modeling CMUTs with Field II. It is shown that rectangular array elements, populated...

  11. Transverse Oscillation Vector Velocity Estimation using a Phased Array Transducer

    DEFF Research Database (Denmark)

    Marcher, Jønne; Pihl, Michael Johannes; Seerup, Gert;

    2012-01-01

    The Transverse Oscillation method has shown its commercial feasibility, providing the user with 2D velocity information. Todays implementation on commercial ultrasound platforms only support linear array transducers and are limited in depth. Extending the implementation to a phased array transduc...... leaves room for optimization. Despite the bias, the method has shown to work and produce reliable results, and 2D velocity estimates are provided within the entire color-box down to a depth of more than 100 mm making vector velocity imaging possible in the entire heart....

  12. 非小细胞肺癌中p-STAT3与Ki67的表达及意义%The expression and significance of phosphorylated signal transducer and activator of transcription 3 and Ki67 in non-small cell lung cancer

    Institute of Scientific and Technical Information of China (English)

    刘培杰

    2010-01-01

    Objective To study the expressions of phosphorylated signal transducer and activator of transcription 3(p-STAT3)and Ki67 in non-small cell lung cancer and their significance in tumor development and progression. Methods The expression of p-STAT3 and Ki67 were detected in 67 lung carcinoma tissues and 41 normal lung tissues by immun histochemical method. Results The positive rate of p-STAT3 in non-small cell lung cancer (67.16%,45/67) was significantly higher than that in normal lung tissue(17.07%,7/41). The positive rate of Ki67 in non-small cell lung cancer (76.12%,51/67) was significantly higher than that in normal lung tissue(7.31%,3/41).The expression of p-STAT3 and Ki67 were associated with clinical stages, lymph node transferation and histological grades(P<0.05). The expression of p-STAT3 and Ki67 were not associated with ages, sex, tumor location or size.The expression of p-STAT3 was positively correlated with Ki67 expression. Conclusions Abnormal activation of p-STAT3, can lead to excessive proliferation of tumor cells and Ki67 is a favorable indicator of proliferation of lung tumor cells, and the combined detection of p-STAT3 and Ki67 may be valuable for diagnosis and treatment of non-small cell lung cancer.%目的 研究磷酸化信号转导与转录活化因子3(p-STAT3)和 Ki67在非小细胞肺癌(NSCLC)发生、发展中的作用及相互关系.方法 采用免疫组织化学S-P法检测67例非小细胞肺癌组织和41例正常肺组织中p-STAT3与Ki67的表达.结果 非小细胞肺癌组织中p-STAT3的阳性表达率(67.16%,45/67)显著高于正常肺组织(17.07%,7/41),Ki67在非小细胞肺癌组织中的表达(76.12%,51/67)高于正常肺组织(7.31%,3/41).p-STAT3和 Ki67的表达与临床分期、有无淋巴转移和分化程度相关(P均<0.05),与患者年龄、性别、肿瘤位置及大小无关.p-STAT3的表达与Ki67表达呈正相关.结论 p-STAT3的异常活化可促进恶性肿瘤细胞的过度增殖,Ki67较好的反应了肺癌

  13. Simple method for measuring vibration amplitude of high power airborne ultrasonic transducer: using thermo-couple.

    Science.gov (United States)

    Saffar, Saber; Abdullah, Amir

    2014-03-01

    Vibration amplitude of transducer's elements is the influential parameters in the performance of high power airborne ultrasonic transducers to control the optimum vibration without material yielding. The vibration amplitude of elements of provided high power airborne transducer was determined by measuring temperature of the provided high power airborne transducer transducer's elements. The results showed that simple thermocouples can be used both to measure the vibration amplitude of transducer's element and an indicator to power transmission to the air. To verify our approach, the power transmission to the air has been investigated by other common method experimentally. The experimental results displayed good agreement with presented approach. PMID:24246149

  14. Determination of the response time of pressure transducers using the direct method

    International Nuclear Information System (INIS)

    The available methods to determine the response time of nuclear safety related pressure transducers are discussed, with emphasis to the direct method. In order to perform the experiments, a Hydraulic Ramp Generator was built. The equipment produces ramp pressure transients simultaneously to a reference transducer and to the transducer under test. The time lag between the output of the two transducers, when they reach a predetermined setpoint, is measured as the time delay of the transducer under test. Some results using the direct method to determine the time delay of pressure transducers (1 E Class Conventional) are presented. (author). 18 refs, 35 figs, 12 tabs

  15. Cantilever deflection measurement and actuation by an nterdigitated transducer

    NARCIS (Netherlands)

    Strambini, E.; Piazza, V.; Pingue, P.; Biasiol, G.; Sorba, L.; Beltram, F.

    2010-01-01

    A scheme that allows all-electrical high-bandwidth readout of a cantilever deflection by means of an integrated interdigitated transducer is presented. The present approach takes advantage of the piezoelectricity of the chosen cantilever substrate material to generate and detect surface-acoustic-wav

  16. Linear synthetic aperture focusing using ultrasonic contact transducers

    International Nuclear Information System (INIS)

    Synthetic aperture processing performed on linear scans with contact ultrasonic transducers can yield useful improvements in lateral resolution of deeply buried defects. Degradation in resolution caused by adverse surface conditions can be minimised by a simple pre-processing normalisation technique. (author)

  17. Characterization of HIFU transducers designed for sonochemistry application: Acoustic streaming.

    Science.gov (United States)

    Hallez, L; Touyeras, F; Hihn, J-Y; Bailly, Y

    2016-03-01

    Cavitation distribution in a High Intensity Focused Ultrasound sonoreactors (HIFU) has been extensively described in the recent literature, including quantification by an optical method (Sonochemiluminescence SCL). The present paper provides complementary measurements through the study of acoustic streaming generated by the same kind of HIFU transducers. To this end, results of mass transfer measurements (electrodiffusional method) were compared to optical method ones (Particle Image Velocimetry). This last one was used in various configurations: with or without an electrode in the acoustic field in order to have the same perturbation of the wave propagation. Results show that the maximum velocity is not located at the focal but shifted near the transducer, and that this shift is greater for high powers. The two cavitation modes (stationary and moving bubbles) are greatly affect the hydrodynamic behavior of our sonoreactors: acoustic streaming and the fluid generated by bubble motion. The results obtained by electrochemical measurements show the same low hydrodynamic activity in the transducer vicinity, the same shift of the active focal toward the transducer, and the same absence of activity in the post-focal axial zone. The comparison with theoretical Eckart's velocities (acoustic streaming in non-cavitating media) confirms a very high activity at the "sonochemical focal", accounted for by wave distortion, which induced greater absorption coefficients. Moreover, the equivalent liquid velocities are one order of magnitude larger than the ones measured by PIV, confirming the enhancement of mass transfer by bubbles oscillation and collapse close to the surface, rather than from a pure streaming effect.

  18. High Temperature Ultrasonic Transducers : Material Selection and Testing

    Science.gov (United States)

    Bar-Cohen, Yoseph; Bruno, Alessandro

    2012-01-01

    The task of my two-months internship was to test different materials to be used to build an high temperature transducer, to develop some prototypes and to test their performance, to assess the reliability of commercial product rated for such a temperature, as well as to collaborate in developing the signal processing code to measure the condensed water levels.

  19. Multilayer piezoelectric transducer models combined with Field II

    DEFF Research Database (Denmark)

    Bæk, David; Willatzen, Morten; Jensen, Jørgen Arendt

    2012-01-01

    One-dimensional and three-dimensional axisymmetric transducer model have been compared to determine their feasibility to predict the volt-to-surface impulse response of a circular Pz27 piezoceramic disc. The ceramic is assumed mounted with silver electrodes, bounded at the outer circular boundary...

  20. Multilayer pieozoelectric transducer models combined with Field II

    DEFF Research Database (Denmark)

    Bæk, David; Willatzen, Morten

    2009-01-01

    boundary with a polymer ring, and submerged into water. The transducer models are developed to account for any external electrical loading impedance in the driving circuit. The models are adapted to calculate the impulse surface acceleration needed by the Field II software in predicting pressure pulses at...

  1. Breast ultrasound tomography with two parallel transducer arrays

    Science.gov (United States)

    Huang, Lianjie; Shin, Junseob; Chen, Ting; Lin, Youzuo; Gao, Kai; Intrator, Miranda; Hanson, Kenneth

    2016-03-01

    Breast ultrasound tomography is an emerging imaging modality to reconstruct the sound speed, density, and ultrasound attenuation of the breast in addition to ultrasound reflection/beamforming images for breast cancer detection and characterization. We recently designed and manufactured a new synthetic-aperture breast ultrasound tomography prototype with two parallel transducer arrays consisting of a total of 768 transducer elements. The transducer arrays are translated vertically to scan the breast in a warm water tank from the chest wall/axillary region to the nipple region to acquire ultrasound transmission and reflection data for whole-breast ultrasound tomography imaging. The distance of these two ultrasound transducer arrays is adjustable for scanning breasts with different sizes. We use our breast ultrasound tomography prototype to acquire phantom and in vivo patient ultrasound data to study its feasibility for breast imaging. We apply our recently developed ultrasound imaging and tomography algorithms to ultrasound data acquired using our breast ultrasound tomography system. Our in vivo patient imaging results demonstrate that our breast ultrasound tomography can detect breast lesions shown on clinical ultrasound and mammographic images.

  2. Nonlinear behaviour of power ultrasonic transducers for food processing

    Science.gov (United States)

    Riera, E.; Cardoni, A.; Acosta, V. M.; Gallego-Juárez, J. A.

    2012-05-01

    Power ultrasonic systems at laboratory and semi-industrial scale are currently investigated to demonstrate the suitability of ultrasonic waves of high-intensity to industrial applications. It has been shown that intense ultrasonic fields trigger a series of mechanisms in the irradiated media that may enhance and/or accelerate a variety of processes in the food sector. Ultrasonic radiators driven by piezoelectric vibrators have been specifically developed for assisting in drying and extraction operations. Successful industrial scale-up of such tuned systems significantly depends on the control of their nonlinear vibration behaviour at high operational power levels. In this paper we investigated experimentally the nonlinear dynamics of two power ultrasonic transducers: a grooved-plate transducer and a cylindrical radiator transducer. Nonlinear mechanisms affecting the dynamic behaviour of both assemblies such as the appearance of harmonics, combination of resonances, or modal interactions, and response saturation are presented. In particular, energy transfers among system modes that may produce the excitation of nontuned resonant frequencies causing heating, noise and even failures of the transducers are identified and characterised.

  3. Candle soot nanoparticles-polydimethylsiloxane composites for laser ultrasound transducers

    Science.gov (United States)

    Chang, Wei-Yi; Huang, Wenbin; Kim, Jinwook; Li, Sibo; Jiang, Xiaoning

    2015-10-01

    Generation of high power laser ultrasound strongly demands the advanced materials with efficient laser energy absorption, fast thermal diffusion, and large thermoelastic expansion capabilities. In this study, candle soot nanoparticles-polydimethylsiloxane (CSNPs-PDMS) composite was investigated as the functional layer for an optoacoustic transducer with high-energy conversion efficiency. The mean diameter of the collected candle soot carbon nanoparticles is about 45 nm, and the light absorption ratio at 532 nm wavelength is up to 96.24%. The prototyped CSNPs-PDMS nano-composite laser ultrasound transducer was characterized and compared with transducers using Cr-PDMS, carbon black (CB)-PDMS, and carbon nano-fiber (CNFs)-PDMS composites, respectively. Energy conversion coefficient and -6 dB frequency bandwidth of the CSNPs-PDMS composite laser ultrasound transducer were measured to be 4.41 × 10-3 and 21 MHz, respectively. The unprecedented laser ultrasound transduction performance using CSNPs-PDMS nano-composites is promising for a broad range of ultrasound therapy applications.

  4. Nonlinear Dynamic Modeling of Langevin-Type Piezoelectric Transducers

    Directory of Open Access Journals (Sweden)

    Nicolás Peréz Alvarez

    2015-11-01

    Full Text Available Langevin transducers are employed in several applications, such as power ultrasound systems, naval hydrophones, and high-displacement actuators. Nonlinear effects can influence their performance, especially at high vibration amplitude levels. These nonlinear effects produce variations in the resonant frequency, harmonics of the excitation frequency, in addition to loss of symmetry in the frequency response and “frequency domain hysteresis”. In this context, this paper presents a simplified nonlinear dynamic model of power ultrasound transducers requiring only two parameters for simulating the most relevant nonlinear effects. One parameter reproduces the changes in the resonance frequency and the other introduces the dependence of the frequency response on the history of the system. The piezoelectric constitutive equations are extended by a linear dependence of the elastic constant on the mechanical displacement amplitude. For introducing the frequency hysteresis, the elastic constant is computed by combining the current value of the mechanical amplitude with the previous state amplitude. The model developed in this work is applied for predicting the dynamic responses of a 26 kHz ultrasonic transducer. The comparison of theoretical and experimental responses, obtained at several input voltages around the tuned frequency, shows a good agreement, indicating that the model can accurately describe the transducer nonlinear behavior.

  5. Multilevel inverter based class D audio amplifier for capacitive transducers

    DEFF Research Database (Denmark)

    Nielsen, Dennis; Knott, Arnold; Andersen, Michael A. E.

    2014-01-01

    The reduced semiconductor voltage stress makes the multilevel inverters especially interesting, when driving capacitive transducers for audio applications. A ± 300 V flying capacitor class D audio amplifier driving a 100 nF load in the midrange region of 0.1-3.5 kHz with Total Harmonic Distortion...

  6. A Novel Rotary Piezoelectric Motor Using First Bending Hybrid Transducers

    Directory of Open Access Journals (Sweden)

    Yingxiang Liu

    2015-08-01

    Full Text Available We report a novel rotary piezoelectric motor using bending transducers in this work. Three transducers are used to drive a disk-shaped rotor together by the elliptical movements of their driving tips; these motions are produced by the hybrid of two first bending vibration modes. The proposed piezoelectric transducer has a simple structure as it only contains an aluminum alloy beam and four pieces of PZT plates. Symmetrical structure is the only necessary condition in the design process as it will ensure the resonance frequencies of the two orthogonal first bending modes are equal. Transducers with first bending resonance frequency of about 53 kHz were fabricated and assembled into a rotary motor. The proposed motor exhibits good performance on speed and torque control. Under a working frequency of 53.2 kHz, the maximum no-load speed and the maximum torque of the prototype are tested to be 53.3 rpm and of 27 mN·m.

  7. Nuclear Radiation Tolerance of Single Crystal Aluminum Nitride Ultrasonic Transducer

    Science.gov (United States)

    Reinhard, Brian; Tittmann, Bernhard R.; Suprock, Andrew

    Ultrasonic technologies offer the potential for high accuracy and resolution in-pile measurement of a range of parameters, including geometry changes, temperature, crack initiation and growth, gas pressure and composition, and microstructural changes. Many Department of Energy-Office of Nuclear Energy (DOE-NE) programs are exploring the use of ultrasonic technologies to provide enhanced sensors for in-pile instrumentation during irradiation testing. For example, the ability of small diameter ultrasonic thermometers (UTs) to provide a temperature profile in candidate metallic and oxide fuel would provide much needed data for validating new fuel performance models, (Rempe et al., 2011; Kazys et al., 2005). These efforts are limited by the lack of identified ultrasonic transducer materials capable of long term performance under irradiation test conditions. To address this need, the Pennsylvania State University (PSU) was awarded an Advanced Test Reactor National Scientific User Facility (ATR NSUF) project to evaluate the performance of promising magnetostrictive and piezoelectric transducers in the Massachusetts Institute of Technology Research Reactor (MITR) up to a fast fluence of at least 1021 n/cm2. The irradiation is also supported by a multi-National Laboratory collaboration funded by the Nuclear Energy Enabling Technologies Advanced Sensors and Instrumentation (NEET ASI) program. The results from this irradiation, which started in February 2014, offer the potential to enable the development of novel radiation tolerant ultrasonic sensors for use in Material Testing Reactors (MTRs). As such, this test is an instrumented lead test and real-time transducer performance data is collected along with temperature and neutron and gamma flux data. Hence, results from this irradiation offer the potential to bridge the gap between proven out-of-pile ultrasonic techniques and in-pile deployment of ultrasonic sensors by acquiring the data necessary to demonstrate the

  8. Lithium niobate transducers for MRI-guided ultrasonic microsurgery.

    Science.gov (United States)

    Kotopoulis, Spiros; Wang, Han; Cochran, Sandy; Postema, Michiel

    2011-08-01

    Focused ultrasound surgery (FUS) is usually based on frequencies below 5 MHz-typically around 1 MHz. Although this allows good penetration into tissue, it limits the minimum lesion dimensions that can be achieved. In this study, we investigate devices to allow FUS at much higher frequencies, in principle, reducing the minimum lesion dimensions. Furthermore, FUS can produce deep-sub-millimeter demarcation between viable and necrosed tissue; high-frequency devices may allow this to be exploited in superficial applications which may include dermatology, ophthalmology, treatment of the vascular system, and treatment of early dysplasia in epithelial tissue. In this paper, we explain the methodology we have used to build high-frequency high-intensity transducers using Y-36°-cut lithium niobate. This material was chosen because its low losses give it the potential to allow very-high-frequency operation at harmonics of the fundamental operating frequency. A range of single-element transducers with center frequencies between 6.6 and 20.0 MHz were built and the transducers' efficiency and acoustic power output were measured. A focused 6.6-MHz transducer was built with multiple elements operating together and tested using an ultrasound phantom and MRI scans. It was shown to increase phantom temperature by 32°C in a localized area of 2.5 x 3.4 mm in the plane of the MRI scan. Ex vivo tests on poultry tissue were also performed and shown to create lesions of similar dimensions. This study, therefore, demonstrates that it is feasible to produce high-frequency transducers capable of high-resolution FUS using lithium niobate. PMID:21859576

  9. Effect of stimuli, transducers and gender on acoustic change complex

    Directory of Open Access Journals (Sweden)

    Hemanth N. Shetty

    2012-08-01

    Full Text Available The objective of this study was to investigate the effect of stimuli, transducers and gender on the latency and amplitude of acoustic change complex (ACC. ACC is a multiple overlapping P1-N1-P2 complex reflecting acoustic changes across the entire stimulus. Fifteen males and 15 females, in the age range of 18 to 25 (mean=21.67 years, having normal hearing participated in the study. The ACC was recorded using the vertical montage. The naturally produced stimuli /sa/ and /si/ were presented through the insert earphone/loud speaker to record the ACC. The ACC obtained from different stimuli presented through different transducers from male/female participants were analyzed using mixed analysis of variance. Dependent t-test and independent t-test were performed when indicated. There was a significant difference in latency of 2N1 at the transition, with latency for /sa/ being earlier; but not at the onset portions of ACC. There was no significant difference in amplitude of ACC between the stimuli. Among the transducers, there was no significant difference in latency and amplitude of ACC, for both /sa/ and /si/ stimuli. Female participants showed earlier latency for 2N1 and larger amplitude of N1 and 2P2 than male participants, which was significant. ACC provides important insight in detecting the subtle spectral changes in each stimulus. Among the transducers, no difference in ACC was noted as the spectra of stimuli delivered were within the frequency response of the transducers. The earlier 2N1 latency and larger N1 and 2P2 amplitudes noticed in female participants could be due to smaller head circumference. The findings of this study will be useful in determining the capacity of the auditory pathway in detecting subtle spectral changes in the stimulus at the level of the auditory cortex.

  10. Effects of curcuma extract-elemene on proliferation, invasion and tumor-associated calcium signal transducer-2 gene expression of various cancer cells%莪术提取物榄香烯对多种癌细胞增殖、侵袭及肿瘤相关钙信号传导蛋白-2基因表达的影响

    Institute of Scientific and Technical Information of China (English)

    祁卫东; 龚丹丹; 钟锡明; 沈荣; 张恒; 范钰

    2014-01-01

    Objective To study the effects and mechanism of curcuma extract-elemene on proliferation,invasion and tumor-associated calcium signal transducer-2 (TROP-2) gene expression of various cancer cells.Methods Human esophageal carcinoma TE-1 and Ca-ES-17 cells,laryngeal squamous cell carcinoma Hep-2 cells,lung cancer NCI-H1975 cells,colorectal cancer SW-480 cells,and prostate cancer PC-3 cells were prepared.After all group cancers were treated with different doses of elemene,and proliferation of cancer cells was studied by methyl thiazol tetrazolium (MTT) assay,and invasion ability was determined by Boyden chamber assay.TROP-2 mRNA level was detected by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR).Results Elemene has an inhibitory effect on all group cancer cells in a dose-dependent manner.The MTT assay showed that the inhibition rate of proliferation was 43.919,189.036,48.690,75.538,35.145,and 31.063 mg/L in TE-1,Ca-ES-17,Hep-2,NCI-H1975,SW-480,and PC-3 cells,respectively.The Boyden chamber test showed that the inhibition rate of invasion was 25.6%,18.9%,23.8%,22.2%,26.5%,and 27.8% in TE-1,Ca-ES-17,Hep-2,NCI-H1975,SW-480,and PC-3,respectively.The FQ-PCR assay showed that the inhibition rate of TROP-2 mRNA was 35.8%,25.6%,32.5%,25.6%,29.8%,and 33.6% in TE-1,Ca-ES-17,Hep-2,NCI-H1975,SW-480,and PC-3,respectively.Conclusion Elemene could reduce cell proliferation and invasion of various cancer cells through down-regulation of TROP-2 gene.%目的 探讨莪术提取物榄香烯对多种人癌细胞增殖、侵袭的影响及其机制.方法 采用不同浓度的榄香烯处理人食管癌TE-1和Ca-ES-17细胞、喉鳞癌Hep-2细胞、肺癌NCI-H1975细胞、大肠癌SW-480细胞、前列腺癌PC-3细胞后,以噻唑蓝(MTT)方法检测癌细胞增殖,以Boyden小室方法检测癌细胞侵袭能力,以实时荧光定量聚合酶链反应(FQ-PCR)方法检测癌细胞肿瘤相关钙信号传导蛋白(TROP)-2

  11. Six-Axis Force-Torque Transducer for Mars 2018 Mission Project

    Data.gov (United States)

    National Aeronautics and Space Administration — A transducer element that is hearty enough for a Mars lander mission needs to be developed so that a six-axis force and torque transducer is possible. The technical...

  12. Eddy Current Transducer Dedicated for Sigma Phase Evaluation in Duplex Stainless Steel

    Directory of Open Access Journals (Sweden)

    Grzegorz Psuj

    2012-01-01

    Full Text Available The paper describes a new transducer dedicated for evaluation of a duplex stainless steel (DSS. Different phases which exist in DSS have influence on mechanical as well as on electrical properties. Therefore, an eddy current transducer was utilized. In order to achieve high sensitivity, a differential type of the transducer was selected. The performance of the transducer was verified by utilizing the samples which had a different amount of sigma phase.

  13. BacMam Virus Transduced Cardiomyoblasts Can Be Used for Myocardial Transplantation Using AP-PEG-A Microcapsules: Molecular Cloning, Preparation, and In Vitro Analysis

    Directory of Open Access Journals (Sweden)

    Arghya Paul

    2010-01-01

    Full Text Available The potential of genetically modified cardiomyoblasts in treating damaged myocardium is well known. However, efficient delivery of these cells is of major concern during treatment. The limiting factors are the massive cell death that occurs soon after their intramyocardial transplantation into the beating heart. To address these problems, we generated recombinant baculoviruses (BacMam viruses which efficiently transduced cardiomyoblast cells under optimized conditions. These genetically modified cells were then protected in a new polymeric microcapsule using poly-ethylene-glycol (PEG, alginate, and poly-L-lysine (PLL polymers for efficient delivery. Results showed that microcapsules maintain cell viability and support cell proliferation for at least 30 days. The capsules exhibit strong immunoprotective potential and have high mechanical and osmotic stability with more than 70% intact capsules. The encased transduced cells showed a rapid transgene expression inside the capsule for at least 15 days. However, preclinical studies are needed to further explore its long-term functional benefits.

  14. Progress towards developing neutron tolerant magnetostrictive and piezoelectric transducers

    International Nuclear Information System (INIS)

    Current generation light water reactors (LWRs), sodium cooled fast reactors (SFRs), small modular reactors (SMRs), and next generation nuclear plants (NGNPs) produce harsh environments in and near the reactor core that can severely tax material performance and limit component operational life. To address this issue, several Department of Energy Office of Nuclear Energy (DOE-NE) research programs are evaluating the long duration irradiation performance of fuel and structural materials used in existing and new reactors. In order to maximize the amount of information obtained from Material Testing Reactor (MTR) irradiations, DOE is also funding development of enhanced instrumentation that will be able to obtain in-situ, real-time data on key material characteristics and properties, with unprecedented accuracy and resolution. Such data are required to validate new multi-scale, multi-physics modeling tools under development as part of a science-based, engineering driven approach to reactor development. It is not feasible to obtain high resolution/microscale data with the current state of instrumentation technology. However, ultrasound-based sensors offer the ability to obtain such data if it is demonstrated that these sensors and their associated transducers are resistant to high neutron flux, high gamma radiation, and high temperature. To address this need, the Advanced Test Reactor National Scientific User Facility (ATR-NSUF) is funding an irradiation, led by PSU, at the Massachusetts Institute of Technology Research Reactor to test the survivability of ultrasound transducers. As part of this effort, PSU and collaborators have designed, fabricated, and provided piezoelectric and magnetostrictive transducers that are optimized to perform in harsh, high flux, environments. Four piezoelectric transducers were fabricated with either aluminum nitride, zinc oxide, or bismuth titanate as the active element that were coupled to either Kovar or aluminum waveguides and two

  15. Progress towards developing neutron tolerant magnetostrictive and piezoelectric transducers

    Science.gov (United States)

    Reinhardt, Brian; Tittmann, Bernhard; Rempe, Joy; Daw, Joshua; Kohse, Gordon; Carpenter, David; Ames, Michael; Ostrovsky, Yakov; Ramuhalli, Pradeep; Montgomery, Robert; Chien, Hualte; Wernsman, Bernard

    2015-03-01

    Current generation light water reactors (LWRs), sodium cooled fast reactors (SFRs), small modular reactors (SMRs), and next generation nuclear plants (NGNPs) produce harsh environments in and near the reactor core that can severely tax material performance and limit component operational life. To address this issue, several Department of Energy Office of Nuclear Energy (DOE-NE) research programs are evaluating the long duration irradiation performance of fuel and structural materials used in existing and new reactors. In order to maximize the amount of information obtained from Material Testing Reactor (MTR) irradiations, DOE is also funding development of enhanced instrumentation that will be able to obtain in-situ, real-time data on key material characteristics and properties, with unprecedented accuracy and resolution. Such data are required to validate new multi-scale, multi-physics modeling tools under development as part of a science-based, engineering driven approach to reactor development. It is not feasible to obtain high resolution/microscale data with the current state of instrumentation technology. However, ultrasound-based sensors offer the ability to obtain such data if it is demonstrated that these sensors and their associated transducers are resistant to high neutron flux, high gamma radiation, and high temperature. To address this need, the Advanced Test Reactor National Scientific User Facility (ATR-NSUF) is funding an irradiation, led by PSU, at the Massachusetts Institute of Technology Research Reactor to test the survivability of ultrasound transducers. As part of this effort, PSU and collaborators have designed, fabricated, and provided piezoelectric and magnetostrictive transducers that are optimized to perform in harsh, high flux, environments. Four piezoelectric transducers were fabricated with either aluminum nitride, zinc oxide, or bismuth titanate as the active element that were coupled to either Kovar or aluminum waveguides and two

  16. Progress towards developing neutron tolerant magnetostrictive and piezoelectric transducers

    Energy Technology Data Exchange (ETDEWEB)

    Reinhardt, Brian; Tittmann, Bernhard [The Pennsylvania State University (United States); Rempe, Joy; Daw, Joshua [Idaho National Laboratory (United States); Kohse, Gordon; Carpenter, David; Ames, Michael; Ostrovsky, Yakov [Massachusetts Institute of Technology (United States); Ramuhalli, Pradeep; Montgomery, Robert [Pacific Northwest National Laboratory (United States); Chien, Hualte [Argonne National Laboratory (United States); Wernsman, Bernard [Bechtel Marine Propulsion Corp (United States)

    2015-03-31

    Current generation light water reactors (LWRs), sodium cooled fast reactors (SFRs), small modular reactors (SMRs), and next generation nuclear plants (NGNPs) produce harsh environments in and near the reactor core that can severely tax material performance and limit component operational life. To address this issue, several Department of Energy Office of Nuclear Energy (DOE-NE) research programs are evaluating the long duration irradiation performance of fuel and structural materials used in existing and new reactors. In order to maximize the amount of information obtained from Material Testing Reactor (MTR) irradiations, DOE is also funding development of enhanced instrumentation that will be able to obtain in-situ, real-time data on key material characteristics and properties, with unprecedented accuracy and resolution. Such data are required to validate new multi-scale, multi-physics modeling tools under development as part of a science-based, engineering driven approach to reactor development. It is not feasible to obtain high resolution/microscale data with the current state of instrumentation technology. However, ultrasound-based sensors offer the ability to obtain such data if it is demonstrated that these sensors and their associated transducers are resistant to high neutron flux, high gamma radiation, and high temperature. To address this need, the Advanced Test Reactor National Scientific User Facility (ATR-NSUF) is funding an irradiation, led by PSU, at the Massachusetts Institute of Technology Research Reactor to test the survivability of ultrasound transducers. As part of this effort, PSU and collaborators have designed, fabricated, and provided piezoelectric and magnetostrictive transducers that are optimized to perform in harsh, high flux, environments. Four piezoelectric transducers were fabricated with either aluminum nitride, zinc oxide, or bismuth titanate as the active element that were coupled to either Kovar or aluminum waveguides and two

  17. STAT3RNA干扰对脑胶质瘤U251细胞活性氧含量及DNA损伤的影响%Effects of signal transducer and activator of transcription 3 RNAi on content of reactive oxygen species and DNA damage in glioma cell

    Institute of Scientific and Technical Information of China (English)

    高玲; 李峰生; 董波; 刘丽卉; 刘青杰; 陈肖华; 毛秉智

    2011-01-01

    Objective To investigate the effects of signal transducer and activator of transcription 3 (STAT3) RNAi on the content of reactive oxygen species (ROS) and the DNA damage in glioma cells.Methods Glioma cells of the line U251 cells were cultured and transfected with STAT3 RNAi plasmid (pSilencer2.1-STAT3,STAT3 group) and pSilencer2.1-GFP (GFP control group) respectively.Part of the U251 cells were irradiated with γ-rays of 60Co as positive control group of smear phenomenon.The levels of ROS and malondialdehyde (MDA) in the cells were detected 24,48,and 72 h later by flow cytometry and fluorescence chamoluminescence analyzer,respectively.The DNA damage in the transfected U251 cells was examined by using single cell gel electrophoresis assay,and the cell cycle distribution was examined using FACS PI staining 12,24,and 36 h later.Results At 24 h after the transfection,the ROS level of the siSTAT3-transfected ceils was 8.91 times that of the control group (F = 89.296,P < 0.05),and returned to the normal level 48 h later.There were not significant differences in the MDA level of the cells 24,48,and 72 h later between the siSTAT3 group and siGFP group.Compared with the 8 Gy irradiation positive group with obvious smear phenomenon,smear phenomenon was shown in part of the ceils in the siSTAT3 group 6 h later,became less 12 h later,and disappeared completely 24 h later.Compared with the control group,lag of S stage rate was 17.22% and the lag of G2/M stage rate was 6.4% 12 h later in the siSTAT-transfected group,and the G0/G1 stage lag rate was 18.44% 24 h later,and the lag of S stage rate was 17.99% 36 h later.Conclusions Inhibition of STAT3 results in the change of oxidoreduction status in glioma cells,as well as damage and reparation of DNA.%目的 探讨STAT3 RNA干扰(RNAi)对脑胶质瘤U251细胞活性氧含量及DNA损伤的影响.方法 利用构建好的STAT3 RNAi载体(pSilencer2.1-STAT3,STAT3组)和pSileneer2.1-GFP(RNAi对照组)分别转染U251

  18. Investigation of Microopto-eletromechanical Angular Velocity and Acceleration Transducers based on Optical Tunneling Effect

    Science.gov (United States)

    Busurin, V. I.; Lwin, Naing Htoo; Tuan, Pham Anh

    In this paper the possibility of microopto-electromechanical (MOEM) angular velocity and acceleration transducers based on optical tunneling effect (OTE) is considered. The generalized model of MOEM transducers with various types of sensing elements (SE) is developed, transfer functions are investigated, and the errors with various design parameters of transducers are estimated.

  19. Amplifier for optimal reflection Coefficient of ultrasound transducer : A study of op amp based circuits for ultrasound transducers, targeted for low reflection Coefficient, high gain, and low noise

    OpenAIRE

    Mainou Gomez, José Francisco

    2012-01-01

    Reverberation is defined as equally-spaced, bright linear echoes resulting from reflection from specular-type interfaces. They are provoked by the acoustic Impedance change between the tissue and transducer front surface. B. Angelsen developed a mathematical approach to correct this ultrasound artifact by coupling the ultrasound transducer with an ideal electrical load in order to obtain zero reflection coefficients on the transducer from face [1]. However, when analyzing Impedance spectrosco...

  20. The Current State of Silicone-Based Dielectric Elastomer Transducers.

    Science.gov (United States)

    Madsen, Frederikke B; Daugaard, Anders E; Hvilsted, Søren; Skov, Anne L

    2016-03-01

    Silicone elastomers are promising materials for dielectric elastomer transducers (DETs) due to their superior properties such as high efficiency, reliability and fast response times. DETs consist of thin elastomer films sandwiched between compliant electrodes, and they constitute an interesting class of transducer due to their inherent lightweight and potentially large strains. For the field to progress towards industrial implementation, a leap in material development is required, specifically targeting longer lifetime and higher energy densities to provide more efficient transduction at lower driving voltages. In this review, the current state of silicone elastomers for DETs is summarised and critically discussed, including commercial elastomers, composites, polymer blends, grafted elastomers and complex network structures. For future developments in the field it is essential that all aspects of the elastomer are taken into account, namely dielectric losses, lifetime and the very often ignored polymer network integrity and stability. PMID:26773231

  1. Determination of Precise Instantaneous Height at Multibeam Transducer

    Institute of Scientific and Technical Information of China (English)

    ZHAO Jianhu; ZHANG Hongmei; John E. Hughes Clarke

    2007-01-01

    To overcome the shortcomings of the traditional multibeam survey and data processing, a new method is presented for the precise determination of the instantaneous height at the multibeam transducer by the blend of GPS height and heave signals. Before signal blend, GPS height and heave signals need to be corrected first to the transducer center by attitude correction. Second, the GPS height needs to be checked and modified by heave check and modification itself. Butterworth and FFT (fast Fourier transformation) were used in the signal blend. Finally, FFT is thought to be appropriate in signal processing. The new method efficiently overcomes the shortcomings of the traditional method, and this is proven well by the MBS (multibeam bathymetric system) experiment.

  2. A vibration energy harvester using magnet/piezoelectric composite transducer

    Science.gov (United States)

    Qiu, Jing; Chen, Hengjia; Wen, Yumei; Li, Ping; Yang, Jin; Li, Wenli

    2014-05-01

    In this research, a vibration energy harvester employing the magnet/piezoelectric composite transducer to convert mechanical vibration energy into electrical energy is presented. The electric output performance of a vibration energy harvester has been investigated. Compared to traditional magnetoelectric transducer, the proposed vibration energy harvester has some remarkable characteristic which do not need binder. The experimental results show that the presented vibration energy harvester can obtain an average power of 0.39 mW for an acceleration of 0.6g at frequency of 38 Hz. Remarkably, this power is a very encouraging power figure that gives the prospect of being able to power a widely range of wireless sensors in wireless sensor network.

  3. Force transducers based on the stress dependence of coercive force

    Science.gov (United States)

    Garshelis, I. J.

    1993-05-01

    An alternative measurement regime for magnetoelastic force transducers, based on variations in coercive field, is described. Hc is shown to be more directly related to the primary magnetic influence of stress, namely, the orientation of effective anisotropy, than conventionally used magnetization related parameters. The stress dependence of Hc is shown to generally reflect opposing factors associated with rotational and wall displacement magnetization reversal processes. In materials wherein Hc≪K/Ms wall motion dominates and if the product of λs/K and yield stress is high enough, large monotonic reductions of Hc with positive (tensile) stress are shown to be possible. A more complex variation of Hc with increasing compression is similarly expected. Experimental results from a transducer having an 18% Ni maraging steel core support these expectations.

  4. Broadband electrostatic acoustic transducer for ultrasonic measurements in liquids.

    Science.gov (United States)

    Cantrell, J H; Heyman, J S; Yost, W T; Torbett, M A; Breazeale, M A

    1979-01-01

    A broadband capacitive electrostatic acoustic transducer (ESAT) has been developed for use in a liquid environment at megahertz frequencies. The ESAT basically consists of a thin conductive membrane stretched over a metallic housing. The membrane functions as the ground plate of a parallel plate capacitor, the other plate being a dc biased electrode recessed approximately 10 mum from the electrically grounded membrane. An ultrasonic wave incident on the membrane varies the membrane-electrode gap spacing and generates an electrical signal proportional to the wave amplitude. The entire assembly is sealed for immersion in a liquid environment. Calibration of the ESAT with incident ultrasonic waves of constant displacement amplitude from 1 to 15 MHz reveals a decrease in signal response with increasing frequency independent of membrane tension. The use of the ESAT as a broadband ultrasonic transducer in liquids with a predictable frequency response is promising.

  5. The Current State of Silicone-Based Dielectric Elastomer Transducers

    DEFF Research Database (Denmark)

    Madsen, Frederikke Bahrt; Daugaard, Anders Egede; Hvilsted, Søren;

    2016-01-01

    Silicone elastomers are promising materials for dielectric elastomer transducers (DETs) due to their superior properties such as high efficiency, reliability and fast response times. DETs consist of thin elastomer films sandwiched between compliant electrodes, and they consti- tute an interesting...... class of transducer due to their inherent lightweight and potentially large strains. For the field to progress towards industrial implementation, a leap in material devel- opment is required, specifically targeting longer lifetime and higher energy densities to provide more efficient transduction at lower...... of the elastomer are taken into account, namely dielectric losses, life- time and the very often ignored polymer network integrity and stability....

  6. Comprehensive helicopter rotor instrumentation - A retrofit approach using miniature transducers

    Science.gov (United States)

    Jacklin, Stephen A.; Mort, Ray; Morrison, Dwayne

    1992-01-01

    This paper reports an approach used to retrofit a set of full-scale main rotor blades with 290 miniature pressure transducers, 46 strain gages, and 24 miniature accelerometers. Normally, in order to avoid disturbing the aerodynamics of the rotor flow field, the pressure instrumentation must be integrally built into the body of the rotor blades. However, using a method developed with NASA, miniature pressure transducers are mounted to the blade exterior surface without degrading the quality of the blade aerodynamics. Moreover, it is estimated that this approach reduced costs by more than 50 percent over building a set of pressure instrumented blades. The aerodynamic measurement objectives are presented as are instrumentation design considerations, type of instrumentation used, assembly process, and the installed instrumentation characteristics.

  7. Electrocaloric Response of Ferroelectric Material Applicable as Electrothermal Transducer

    Directory of Open Access Journals (Sweden)

    Saber Mohammadi

    2013-01-01

    Full Text Available Electrocaloric response of the PMN-10PT is measured experimentally and compared with the numerical results. Based on the compatibility of the experimental and numerical results, feasibility of using ferroelectric materials as an electrothermal transducer has been investigated. In this study, electrocaloric response of three different ferroelectric capacitors (PMN-10PT, PMN-25PT, and PZN-4.5PT under an applied periodic electric field have been investigated. Alternative switching of the electrocaloric elements with specific boundary conditions generates a directed heat flux. It can be concluded that each ferroelectric material can be used as a transducer in a special temperature range that in which it has good electrocaloric response.

  8. Fiber optic ultrasound transducers with carbon/PDMS composite coatings

    Science.gov (United States)

    Mosse, Charles A.; Colchester, Richard J.; Bhachu, Davinder S.; Zhang, Edward Z.; Papakonstantinou, Ioannis; Desjardins, Adrien E.

    2014-03-01

    Novel ultrasound transducers were created with a composite of carbon nanotubes (CNTs) and polydimethylsiloxane (PDMS) that was dip coated onto the end faces of optical fibers. The CNTs were functionalized with oleylamine to allow for their dissolution in xylene, a solvent of PDMS. Ultrasound pulses were generated by illuminating the composite coating with pulsed laser light. At distances of 2 to 16 mm from the end faces, ultrasound pressures ranged from 0.81 to 0.07 MPa and from 0.27 to 0.03 MPa with 105 and 200 μm core fibers, respectively. Using an optical fiber hydrophone positioned adjacent to the coated 200 µm core optical fiber, ultrasound reflectance measurements were obtained from the outer surface of a sheep heart ventricle. The results of this study suggest that ultrasound transducers that comprise optical fibers with CNT-PDMS composite coatings may be suitable for miniature medical imaging probes.

  9. Optimization of large-scale fabrication of dielectric elastomer transducers

    DEFF Research Database (Denmark)

    Hassouneh, Suzan Sager

    Polypower A/S employs a large-scale process for manufacturing DE films with one-sided corrugated surfaces. The DEs are manufactured by coating an elastomer mixture to a corrugated carrier web, thereby imprinting the corrugations onto the elastomer. The corrugated elastomer is then sputtered with metal...... electrodes on the corrugated surface, and due to these corrugated surfaces the metal electrodes maintain conductivities up to more than 100% strain of the elastomer film. The films are then laminated in multiple layers to fabricate DE transducers. However, the current manufacturing process is not trouble......-free, and two issues in particular have great influence on the performance of DE transducers. The first issue is the release of the corrugated elastomer film from the carrier web, due to the large surface area and flexible nature of the elastomer film, while the second issue relates to the lamination of DE...

  10. Simulating arbitrary-geometry ultrasound transducers using triangles

    DEFF Research Database (Denmark)

    Jensen, Jørgen Arendt

    1996-01-01

    -echo field. The spatial impulse response has only been determined analytically for a few geometries and using apodization over the transducer surface generally makes it impossible to find the response analytically. A popular approach to find the general field is thus to split the aperture into small...... number of transducers can be defined and their properties manipulated. The program can calculate all types of ultrasound fields, and can also be used for simulating B-mode and color flow images. Both the focusing and apodization can be set to be dynamic with respect to time, and it is thus possible to......-field response of a rectangle, as the triangle equations are far more complicated. This approach is therefore best suited for accurate modeling of fields, whereas the rectangle program is better suited to make fast simulated images, since contributions from many scatterers are summed here and the error is...

  11. Local Area Damage Detection in Composite Structures Using Piezoelectric Transducers

    OpenAIRE

    Lichtenwalner, Peter F.; Sofge, Donald A.

    2007-01-01

    An integrated and automated smart structures approach for structural health monitoring is presented, utilizing an array of piezoelectric transducers attached to or embedded within the structure for both actuation and sensing. The system actively interrogates the structure via broadband excitation of multiple actuators across a desired frequency range. The structure's vibration signature is then characterized by computing the transfer functions between each actuator/sensor pair, and compared t...

  12. Characterization of HIFU transducers designed for sonochemistry application: Acoustic streaming.

    Science.gov (United States)

    Hallez, L; Touyeras, F; Hihn, J-Y; Bailly, Y

    2016-03-01

    Cavitation distribution in a High Intensity Focused Ultrasound sonoreactors (HIFU) has been extensively described in the recent literature, including quantification by an optical method (Sonochemiluminescence SCL). The present paper provides complementary measurements through the study of acoustic streaming generated by the same kind of HIFU transducers. To this end, results of mass transfer measurements (electrodiffusional method) were compared to optical method ones (Particle Image Velocimetry). This last one was used in various configurations: with or without an electrode in the acoustic field in order to have the same perturbation of the wave propagation. Results show that the maximum velocity is not located at the focal but shifted near the transducer, and that this shift is greater for high powers. The two cavitation modes (stationary and moving bubbles) are greatly affect the hydrodynamic behavior of our sonoreactors: acoustic streaming and the fluid generated by bubble motion. The results obtained by electrochemical measurements show the same low hydrodynamic activity in the transducer vicinity, the same shift of the active focal toward the transducer, and the same absence of activity in the post-focal axial zone. The comparison with theoretical Eckart's velocities (acoustic streaming in non-cavitating media) confirms a very high activity at the "sonochemical focal", accounted for by wave distortion, which induced greater absorption coefficients. Moreover, the equivalent liquid velocities are one order of magnitude larger than the ones measured by PIV, confirming the enhancement of mass transfer by bubbles oscillation and collapse close to the surface, rather than from a pure streaming effect. PMID:26585023

  13. Development of an omnidirectional SH0 piezoceramic transducer

    Science.gov (United States)

    Belanger, Pierre; Boivin, Guillaume

    2016-02-01

    Ultrasonic guided waves are now routinely used in non-destructive evaluation. In plate-like structures, three fundamental modes can propagate, namely A0, S0 and SH0. Most of the guided wave literature has thus far focused on the use of A0 and/or S0 because these modes are easy to generate in plate-like structures using standard piezoceramic transducers. Yet, at low frequency, A0 and S0 are dispersive. The consequence of dispersion is that signal processing becomes complex for long propagation distances. SH0, on the other hand, has the particularity of being the only non-dispersive guided wave mode. Omnidirectional transduction of SH0 requires a rotational surface stress which cannot be easily generated using standard piezoceramic transducers. This project investigated the use of piezoceramic shear plates cut into six trapezoids bonded to a plate in order to form a discretized circle. The individual elements of the hexagonal shaped transducer were synchronized to generate shear surface stress simultaneously. The external diameter of the discretized circle was chosen to be half the SH0 wavelength at the desired centre frequency. Finite element simulations using the Comsol Multiphysics environment showed that in a 1.6 mm aluminium plate the modal selectivity of the transducer was more than 30 dB at 100 kHz. The concept was then validated experimentally on a 1.6 mm aluminium plate. The 3D experimental displacement field was measured using a laser Doppler vibrometer system. The experimental modal selectivity was 20 dB.

  14. Digital objects as "transducers" in scientific web publishing

    OpenAIRE

    Koltzenburg, Claudia

    2007-01-01

    Scientific web publishing offers an attractive bundle of phenomena for feminist technoscientific investigation. This article focuses on research articles in scientific journals and aims at identifying a range of exclusionary practices in the current publishing system, which need to be critically addressed. For this purpose, the functionalities of digital objects are studied using the analogy of a piezoelectric crystal as a transducer in obstetric ultrasonography (Karen Barad 2001). This is em...

  15. Progress towards developing neutron tolerant magnetostrictive and piezoelectric transducers

    Energy Technology Data Exchange (ETDEWEB)

    Reinhardt, Brian [Pennsylvania State Univ., University Park, PA (United States). Dept. of Engineering Science and Mechanics; Tittmann, Bernhard [Pennsylvania State Univ., University Park, PA (United States). Dept. of Engineering Science and Mechanics; Rempe, Joy [Idaho National Lab. (INL), Idaho Falls, ID (United States); Daw, Joshua [Idaho National Lab. (INL), Idaho Falls, ID (United States); Kohse, Gordon [MIT (Massachusetts Inst. of Technology), Cambridge, MA (United States). MIT Nuclear Reactor Lab.; Carpenter, David [MIT (Massachusetts Inst. of Technology), Cambridge, MA (United States). MIT Nuclear Reactor Lab.; Ames, Micheal [MIT (Massachusetts Inst. of Technology), Cambridge, MA (United States). MIT Nuclear Reactor Lab.; Ostrovsky, Yakov [MIT (Massachusetts Inst. of Technology), Cambridge, MA (United States). MIT Nuclear Reactor Lab.; Ramuhalli, Pradeep [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Montgomery, Robert [Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Chien, Hualte [Argonne National Lab. (ANL), Argonne, IL (United States); Wernsman, Bernard [Bettis Atomic Power Lab. (BAPL), West Mifflin, PA (United States). Bechtel Marine Propulsion Corp.

    2014-07-01

    Current generation light water reactors (LWRs), sodium cooled fast reactors (SFRs), small modular reactors (SMRs), and next generation nuclear plants (NGNPs) provide harsh environments in and near the core that can severely test material performance and limit their operational life. To address this issue, several Department of Energy Office of Nuclear Energy (DOE-NE) research programs are evaluating the long duration radiation performance of fuels and materials. In To reduce the amount of Material and Test Reactor (MTR) irradiations required, DOE is also funding development of enhanced instrumentation that will be able to obtain data, with unprecedented accuracy and resolution, that are required to validate new multi-scale multiphysics modeling tools . It is not feasible to obtain such data with the current state of instrumentation technology. To address this need, PSU and collaborators have started an experiment to test the potential for utilizing ultrasonic instruments in-pile. Ultrasonic sensors must be resistant to high neutron flux, high gamma radiation, and high temperature. PSU and collaborators have designed, fabricated, and started to irradiate piezoelectric and magnetostrictive transducers designed to perform in such harsh environments. Three piezoelectric transducers were fabricated with aluminum nitride, zinc oxide, and bismuth titanate as the active element. The transducers are coupled kovar and aluminum waveguides of which pulse-echo ultrasonic measurements are made in-situ. Two magnetostrictive transducers were fabricated with Remendur and Arnokrome as the active elements. These devices will be pulsed and monitored in-situ. (1) Selection of candidate sensor materials as well as optimization of test assembly parameters (2) High temperature benchmark testing and (3) initial data from the irradiation will be reported.

  16. Progress towards developing neutron tolerant magnetostrictive and piezoelectric transducers

    International Nuclear Information System (INIS)

    Current generation light water reactors (LWRs), sodium cooled fast reactors (SFRs), small modular reactors (SMRs), and next generation nuclear plants (NGNPs) provide harsh environments in and near the core that can severely test material performance and limit their operational life. To address this issue, several Department of Energy Office of Nuclear Energy (DOE-NE) research programs are evaluating the long duration radiation performance of fuels and materials. In To reduce the amount of Material and Test Reactor (MTR) irradiations required, DOE is also funding development of enhanced instrumentation that will be able to obtain data, with unprecedented accuracy and resolution, that are required to validate new multi-scale multiphysics modeling tools . It is not feasible to obtain such data with the current state of instrumentation technology. To address this need, PSU and collaborators have started an experiment to test the potential for utilizing ultrasonic instruments in-pile. Ultrasonic sensors must be resistant to high neutron flux, high gamma radiation, and high temperature. PSU and collaborators have designed, fabricated, and started to irradiate piezoelectric and magnetostrictive transducers designed to perform in such harsh environments. Three piezoelectric transducers were fabricated with aluminum nitride, zinc oxide, and bismuth titanate as the active element. The transducers are coupled kovar and aluminum waveguides of which pulse-echo ultrasonic measurements are made in-situ. Two magnetostrictive transducers were fabricated with Remendur and Arnokrome as the active elements. These devices will be pulsed and monitored in-situ. (1) Selection of candidate sensor materials as well as optimization of test assembly parameters (2) High temperature benchmark testing and (3) initial data from the irradiation will be reported.

  17. Differential scanning calorimetry of superelastic Nitinol for tunable cymbal transducers

    OpenAIRE

    Feeney, Andrew; Lucas, Margaret

    2015-01-01

    Recent research has shown that estimations of the transformation temperatures of superelastic Nitinol using differential scanning calorimetry can be inaccurate, in part, due to the residual stress in the material. Superelastic Nitinol is selected as the end-cap material in a tunable cymbal transducer. The differential scanning calorimetry accuracy is initially probed by comparing transformation temperature measurements of cold-worked superelastic Nitinol with the same material after an anneal...

  18. Engine Oil Condition Monitoring Using High Temperature Integrated Ultrasonic Transducers

    OpenAIRE

    Jeff Bird; Cheng-Kuei Jen; Zhigang Sun; Pierre Sammut; Brian Galeote; Makiko Kobayashi; Kuo-Ting Wu; Nezih Mrad

    2011-01-01

    The present work contains two parts. In the first part, high temperature integrated ultrasonic transducers (IUTs) made of thick piezoelectric composite films, were coated directly onto lubricant oil supply and sump lines of a modified CF700 turbojet engine. These piezoelectric films were fabricated using a sol-gel spray technology. By operating these IUTs in transmission mode, the amplitude and velocity of transmitted ultrasonic waves across the flow channel of the lubricant oil in supply and...

  19. Forward and Backward Application of Symbolic Tree Transducers

    OpenAIRE

    Fülöp, Zoltán; Vogler, Heiko

    2012-01-01

    We consider symbolic tree automata (sta) and symbolic tree transducers (stt). We characterize s-recognizable tree languages (which are the tree languages recognizable by sta) in terms of (classical) recognizable tree languages and relabelings. We prove that sta and the recently introduced variable tree automata are incomparable with respect to their recognition power. We define symbolic regular tree grammars and characterize s-regular tree languages in terms of regular tree languages and rela...

  20. Differential Pressure Transducer for Corrosion Monitoring of Iron

    OpenAIRE

    Amar Prasad Yadav

    2014-01-01

    In this study, differential pressure transducer (DPT) has been applied as an alternate corrosion monitoring device for monitoring corrosion of iron in atmospheric environment by measuring very small changes in the amount of oxygen. The result of corrosion current obtained from DPT method has been compared with that obtained from AC impedance method. The difference in the value of corrosion current obtained from these two methods was attributed to the error in choosing the value of proportiona...

  1. TREM2-transduced myeloid precursors mediate nervous tissue debris clearance and facilitate recovery in an animal model of multiple sclerosis.

    Directory of Open Access Journals (Sweden)

    Kazuya Takahashi

    2007-04-01

    Full Text Available BACKGROUND: In multiple sclerosis, inflammation can successfully be prevented, while promoting repair is still a major challenge. Microglial cells, the resident phagocytes of the central nervous system (CNS, are hematopoietic-derived myeloid cells and express the triggering receptor expressed on myeloid cells 2 (TREM2, an innate immune receptor. Myeloid cells are an accessible source for ex vivo gene therapy. We investigated whether myeloid precursor cells genetically modified to express TREM2 affect the disease course of experimental autoimmune encephalomyelitis (EAE, an animal model of multiple sclerosis. METHODS AND FINDINGS: EAE was induced in mice by immunization with a myelin autoantigen. Intravenous application of TREM2-transduced bone marrow-derived myeloid precursor cells at the EAE peak led to an amelioration of clinical symptoms, reduction in axonal damage, and prevention of further demyelination. TREM2-transduced myeloid cells applied intravenously migrated into the inflammatory spinal cord lesions of EAE-diseased mice, showed increased lysosomal and phagocytic activity, cleared degenerated myelin, and created an anti-inflammatory cytokine milieu within the CNS. CONCLUSIONS: Intravenously applied bone marrow-derived and TREM2-tranduced myeloid precursor cells limit tissue destruction and facilitate repair within the murine CNS by clearance of cellular debris during EAE. TREM2 is a new attractive target for promotion of repair and resolution of inflammation in multiple sclerosis and other neuroinflammatory diseases.

  2. Ultrasonic Heat Transfer Enhancement Using a Horn-Type Transducer

    Science.gov (United States)

    Nomura, Shinfuku; Yamamoto, Akira; Murakami, Koichi

    2002-05-01

    The purpose of this study is to clarify experimentally the influence of streaming induced by ultrasonic vibration on heat transfer using a horn-type ultrasonic vibrator. A horn tip of 6 mm diameter and 60.7 kHz resonant frequency was used as the ultrasonic transducer. Heat transfer experiments for a downward-facing horizontal heating surface with ultrasonic vibration from below were carried out in a natural convection region. The acoustic jet in the water from the horn tip of the transducer regarded as a nozzle exit was induced by this transducer, and as a result, up to a ten-fold increase in heat transfer coefficient was obtained by application of 20 W in both tap water and degassed water. It was found that the mechanism of heat transfer enhancement by ultrasonic vibration in tap water can be classified into four categories. In degassed water, heat transfer enhancement is influenced not by the acoustic jet, but by small-scale perturbations by cavitation microjets.

  3. MEMS ultrasonic transducer for monitoring of steel structures

    Science.gov (United States)

    Jain, Akash; Greve, David W.; Oppenheim, Irving J.

    2002-06-01

    Ultrasonic methods can be used to monitor crack propagation, weld failure, or section loss at critical locations in steel structures. However, ultrasonic inspection requires a skilled technician, and most commonly the signal obtained at any inspection is not preserved for later use. A preferred technology would use a MEMS device permanently installed at a critical location, polled remotely, and capable of on-chip signal processing using a signal history. We review questions related to wave geometry, signal levels, flaw localization, and electromechanical design issues for microscale transducers, and then describe the design, characterization, and initial testing of a MEMS transducer to function as a detector array. The device is approximately 1-cm square and was fabricated by the MUMPS process. The chip has 23 sensor elements to function in a phased array geometry, each element containing 180 hexagonal polysilicon diaphragms with a typical leg length of 49 microns and an unloaded natural frequency near 3.5 MHz. We first report characterization studies including capacitance-voltage measurements and admittance measurements, and then report initial experiments using a conventional piezoelectric transducer for excitation, with successful detection of signals in an on-axis transmission experiment and successful source localization from phased array performance in an off-axis transmission experiment.

  4. An Analysis of Transducer Mass Loading Effect Inshaker Testing

    Directory of Open Access Journals (Sweden)

    A. D. Karle

    2014-06-01

    Full Text Available Modal Analysis has been a developing science in the experimental evaluation of the dynamic properties of the structures. Frequency Response Function (FRF is one of the major steps in modal analysis. Measured frequency response functions (FRFs are used to extract modal parameters. It is also known that the accuracy and the reliability of various analyses using the measured FRFs depend strongly on the quality of measured data. It is well known that the quality of measured frequency response functions (FRFs is adversely affected by many factors, most significant sources being noise and systematic errors like mass loading effects of transducers. A transducer mounted on a vibrating system changes the dynamics of the structure due to the addition of extra mass and introduces errors into measured FRFs. One problem with this is the production of unrealistic results, which cause the measured resonant frequencies to be less than the correct values. These errors also lead to incorrect prediction of modal parameters. In many situations, the mass loading effect is ignored in the analytical and experimental process, based on a usual assumption that the transducer mass is negligible compared to that of the structure under test. However, when light-weighted structures are investigated, this effect can be significant.

  5. Efficient Driving of Piezoelectric Transducers Using a Biaxial Driving Technique.

    Directory of Open Access Journals (Sweden)

    Samuel Pichardo

    Full Text Available Efficient driving of piezoelectric materials is desirable when operating transducers for biomedical applications such as high intensity focused ultrasound (HIFU or ultrasound imaging. More efficient operation reduces the electric power required to produce the desired bioeffect or contrast. Our preliminary work [Cole et al. Journal of Physics: Condensed Matter. 2014;26(13:135901.] suggested that driving transducers by applying orthogonal electric fields can significantly reduce the coercivity that opposes ferroelectric switching. We present here the experimental validation of this biaxial driving technique using piezoelectric ceramics typically used in HIFU. A set of narrow-band transducers was fabricated with two sets of electrodes placed in an orthogonal configuration (following the propagation and the lateral mode. The geometry of the ceramic was chosen to have a resonance frequency similar for the propagation and the lateral mode. The average (± s.d. resonance frequency of the samples was 465.1 (± 1.5 kHz. Experiments were conducted in which each pair of electrodes was driven independently and measurements of effective acoustic power were obtained using the radiation force method. The efficiency (acoustic/electric power of the biaxial driving method was compared to the results obtained when driving the ceramic using electrodes placed only in the pole direction. Our results indicate that the biaxial method increases efficiency from 50% to 125% relative to the using a single electric field.

  6. Deconvolution based photoacoustic reconstruction for directional transducer with sparsity regularization

    Science.gov (United States)

    Moradi, Hamid; Tang, Shuo; Salcudean, Septimiu E.

    2016-03-01

    We define a deconvolution based photoacoustic reconstruction with sparsity regularization (DPARS) algorithm for image restoration from projections. The proposed method is capable of visualizing tissue in the presence of constraints such as the specific directivity of sensors and limited-view Photoacoustic Tomography (PAT). The directivity effect means that our algorithm treats the optically-generated ultrasonic waves based on which direction they arrive at the transducer. Most PA image reconstruction methods assume that sensors have omni-directional response; however, in practice, the sensors show higher sensitivity to the ultrasonic waves coming from one specific direction. In DPARS, the sensitivity of the transducer to incoming waves from different directions are considered. Thus, the DPARS algorithm takes into account the relative location of the absorbers with respect to the transducers, and generates a linear system of equations to solve for the distribution of absorbers. The numerical conditioning and computing times are improved by the use of a sparse Discrete Fourier Transform (DCT) representation of the distribution of absorption coefficients. Our simulation results show that DPARS outperforms the conventional Delay-and-Sum reconstruction method in terms of CNR and RMS errors. Experimental results confirm that DPARS provides images with higher resolution than DAS.

  7. Dual-Frequency Piezoelectric Transducers for Contrast Enhanced Ultrasound Imaging

    Directory of Open Access Journals (Sweden)

    K. Heath Martin

    2014-11-01

    Full Text Available For many years, ultrasound has provided clinicians with an affordable and effective imaging tool for applications ranging from cardiology to obstetrics. Development of microbubble contrast agents over the past several decades has enabled ultrasound to distinguish between blood flow and surrounding tissue. Current clinical practices using microbubble contrast agents rely heavily on user training to evaluate degree of localized perfusion. Advances in separating the signals produced from contrast agents versus surrounding tissue backscatter provide unique opportunities for specialized sensors designed to image microbubbles with higher signal to noise and resolution than previously possible. In this review article, we describe the background principles and recent developments of ultrasound transducer technology for receiving signals produced by contrast agents while rejecting signals arising from soft tissue. This approach relies on transmitting at a low-frequency and receiving microbubble harmonic signals at frequencies many times higher than the transmitted frequency. Design and fabrication of dual-frequency transducers and the extension of recent developments in transducer technology for dual-frequency harmonic imaging are discussed.

  8. Focused intravascular ultrasonic probe using dimpled transducer elements.

    Science.gov (United States)

    Chen, Y; Qiu, W B; Lam, K H; Liu, B Q; Jiang, X P; Zheng, H R; Luo, H S; Chan, H L W; Dai, J Y

    2015-02-01

    High-frequency focused intravascular ultrasonic probes were fabricated in this study using dimple technique based on PMN-PT single crystal and lead-free KNN-KBT-Mn ceramic. The center frequency, bandwidth, and insertion loss of the PMN-PT transducer were 34 MHz, 75%, and 22.9 dB, respectively. For the lead-free probe, the center frequency, bandwidth, and insertion loss were found to be 40 MHz, 72%, and 28.8 dB, respectively. The ultrasonic images of wire phantom and vessels with good resolution were obtained to evaluate the transducer performance. The -6 dB axial and lateral resolutions of the PMN-PT probe were determined to be 58 μm and 131 μm, respectively. For the lead-free probe, the axial and lateral resolutions were found to be 44 μm and 125 μm, respectively. These results suggest that the mechanical dimpling technique has good potential in preparing focused transducers for intravascular ultrasound applications. PMID:25108608

  9. Analyzing a Vibrating Wire Transducer using Coupled Resonator Circuits

    Directory of Open Access Journals (Sweden)

    POP, S.

    2015-08-01

    Full Text Available This paper intends to be an approach on the vibrating wire transducer from the perspective of the necessary rules used for a correct measurement procedure. There are several studies which analyze the vibrating wire transducer as a mechanical system. However, a comparative time-domain analysis between the mechanical and the electrical model is lacking. The transducer analysis is based on a theoretical analysis of the equivalent circuit, on both excitation and response time intervals. The electrical model consists of two magnetic coupled resonating circuits. When connected to an excitation source, there will be an energy transfer from the coil to the wire. The maximum energy transfer will occur at the vibrating wire's frequency of resonance. Using the transient regime analysis, it has been proven that, in the response time interval - when the wire vibrates freely, the current through the circuit that models the wire describes the oscillating movement of the wire. A complex signal is obtained, that contains both coil's and wire's frequencies of resonance, strongly dependent with theirs parasitic elements. The mathematical analysis highlights the similarity between mechanical and electrical model and the procedures in order to determine the wire frequency of resonance from the output signal.

  10. Development of ultrasonic testing equipment incorporating electromagnetic acoustic transducer

    International Nuclear Information System (INIS)

    This paper describes an automatic flaw detection equipment and heat-resistant ultrasonic transducer for plate thickness measurement. The automatic flaw detection equipment is used during in-service inspection. It comprises an angle-beam electromagnetic acoustic transducer (EMAT), mounted on a vehicle, for scanning the pipe surface to be inspected. The EMAT functions without direct contact with the pipe surface through a coupling liquid, the vehicle does not require a guide track installed on the pipe surface, since it is equipped with magnetic wheels that adhere to the pipe, permitting it to travel along the circumferential weld joint of a carbon steel pipe. Another heat-resistant ultrasonic transducer is a normal beam EMAT and is used during plant operation. As a result, the automatic flaw detection equipment could detect a 1 mm deep notch cut on a test piece of 25 mm thick carbon steel plate. The vehicle location accuracy on the piping was ±2 mm. The normal beam EMAT could measure the plate thickness, within ±0.3 mm accuracy for the range of plate thickness 4 mm to 12 mm at 300degC. (author)

  11. Flexible PVDE comb transducers for excitation of axisymmetric guided waves in pipe

    International Nuclear Information System (INIS)

    Flexible PVDF pipe comb transducers are easy to install by wrapping around any size pipe. It is possible to mechanically couple these transducers to the pipe thereby eliminating the need to bond electrodes to the film and couple the transducer to the pipe. The simple fabrication process, installation, and affordability of these transducers makes them realistic candidates for condition based monitoring of some critical pipeline applications. These transducers are capable of exciting lower order axisymmetric modes with minimal radial displacement and maximum axial displacement as well as modes with both surface displacement components. This versatility is extremely important since under certain loading conditions modes with significant radial displacement are almost completely attenuated.

  12. Tunable-angle wedge transducer for improved acoustophoretic control in a microfluidic chip

    DEFF Research Database (Denmark)

    Iranmanesh, I.; Barnkob, Rune; Bruus, Henrik;

    2013-01-01

    coupling angle, and transducer actuation method (single-frequency actuation or frequency-modulation actuation). The energy-density analysis is based on measuring the transmitted light intensity through a microfluidic channel filled with a suspension of 5 µm diameter beads and the results with the tunable...... uniform particle patterns with average acoustic energy densities comparable to those obtained using single-frequency actuation.......We present a tunable-angle wedge ultrasound transducer for improved control of microparticle acoustophoresis in a microfluidic chip. The transducer is investigated by analyzing the pattern of aligned particles and induced acoustic energy density while varying the transducer geometry, transducer...

  13. Flat HIFU transducer with a sawtooth-shaped ultrasound radiation face

    Science.gov (United States)

    Son, Keon-Ho; Cho, Young-Ki; Kim, Dae-Seung; Kim, Myung-Deok; Kang, Kook-Jin

    2013-10-01

    High-intensity focused ultrasound (HIFU) transducers are spherically-curved in order to obtain a high intensity gain of the converged ultrasound energy at the geometrical focus. Ultrasound imaging devices monitor the procedure of HIFU treatment in ultrasound-guided HIFU systems where the image probe is positioned at the apex of the HIFU transducer, which has a spherical surface. However, the remote image probe's location yields a poor image quality compared to that obtained using conventional ultrasound imaging where the image probe is in direct contact with the surface. A phased array HIFU transducer with a new structure is suggested to overcome this limitation. The centers of the array elements are distributed over the flat surface of the transducer. However, the elements are tilted to form a geometrical focus, like a transducer with a spherically-curved surface, to obtain a high focal gain. The cross-section of the ultrasound radiation face of the transducer resembles the teeth of a saw. The acoustic field emitted from this transducer was simulated in order to design and produce the transducer. The simulation was compared with the measured sound field to verify that the transducer was correctly manufactured and designed; subsequently, the acoustic power was measured, and ultrasound images were obtained through the installation of an image probe on the transducer, which confirmed the application of this transducer to HIFU treatment.

  14. Numerical simulation of ultrasound thermotherapy of brain with a scanned focus transducer

    Science.gov (United States)

    Behnia, Sohrab; Ghalichi, Farzan; Jafari, Amin; Bonabi, Ashkan

    2005-04-01

    Brain tumors are one of the most difficult ones to treat. The margin between destruction of the tumor and damage to the surrounding tissue is narrow in the brain. Ultrasound could be an effective treatment because of its ability to propagate deep in tissue and induce temperature rise at the focus while leaving the surrounding tissue intact. This study investigates whether using a fix-focus transducer could destroy brain tumor cells, in a cost effective manner which reduces the treatment time significantly. In this work an appropriate fix-focus transducer was designed considering effective parameters and limitations which are dominant in this case. Then a real 2-D brain model was constructed from a MR image. A piece of the skull bone has been removed to allow ultrasound to propagate into the brain. The resultant pressure field and the temperature rise were calculated by Rayleigh integral and bio-heat equation on the model. The obtained results were promising indicating that toxic temperatures could be obtained in short treatment times. This could be of great advantage especially in treating primary brain tumors.

  15. Analytical modeling and experimental validation of a V-shape piezoelectric ultrasonic transducer

    Science.gov (United States)

    Li, Xiaoniu; Yao, Zhiyuan

    2016-07-01

    In this paper, an analytical model of a V-shape piezoelectric ultrasonic transducer is presented. The V-shape piezoelectric ultrasonic transducer has been widely applied to the piezoelectric actuator (ultrasonic motor), ultrasonic aided fabrication, sensor, and energy harvesting device. The V-shape piezoelectric ultrasonic transducer consists of two Langevin-type transducers connected together through a coupling point with a certain coupling angle. Considering the longitudinal and lateral movements of a single beam, the symmetrical and asymmetrical modals of the V-shape piezoelectric ultrasonic transducer are calculated. By using Hamilton–Lagrange equations, the electromechanical coupling model of the V-shape piezoelectric ultrasonic transducer is proposed. The influence of the coupling angle and cross-section on modal characteristics and electromechanical coupling coefficient are analyzed by the analytical model. A prototype of the V-shape piezoelectric ultrasonic transducer is fabricated, and the results of the experiments are in good agreement with the analytical model.

  16. A Integrated Circuit for a Biomedical Capacitive Pressure Transducer

    Science.gov (United States)

    Smith, Michael John Sebastian

    Medical research has an urgent need for a small, accurate, stable, low-power, biocompatible and inexpensive pressure sensor with a zero to full-scale range of 0-300 mmHg. An integrated circuit (IC) for use with a capacitive pressure transducer was designed, built and tested. The random pressure measurement error due to resolution and non-linearity is (+OR-)0.4 mmHg (at mid-range with a full -scale of 300 mmHg). The long-term systematic error due to falling battery voltage is (+OR-)0.6 mmHg. These figures were calculated from measurements of temperature, supply dependence and non-linearity on completed integrated circuits. The sensor IC allows measurement of temperature to (+OR-)0.1(DEGREES)C to allow for temperature compensation of the transducer. Novel micropower circuit design of the system components enabled these levels of accuracy to be reached. Capacitance is measured by a new ratiometric scheme employing an on -chip reference capacitor. This method greatly reduces the effects of voltage supply, temperature and manufacturing variations on the sensor circuit performance. The limits on performance of the bandgap reference circuit fabricated with a standard bipolar process using ion-implanted resistors were determined. Measurements confirm the limits of temperature stability as approximately (+OR-)300 ppm/(DEGREES)C. An exact analytical expression for the period of the Schmitt trigger oscillator, accounting for non-constant capacitor charging current, was formulated. Experiments to test agreement with theory showed that prediction of the oscillator period was very accurate. The interaction of fundamental and practical limits on the scaling of the transducer size was investigated including a correction to previous theoretical analysis of jitter in an RC oscillator. An areal reduction of 4 times should be achievable.

  17. Vibration control via stiffness switching of magnetostrictive transducers

    Science.gov (United States)

    Scheidler, Justin J.; Asnani, Vivake M.; Dapino, Marcelo J.

    2016-04-01

    In this paper, a computational study is presented of structural vibration control that is realized by switching a magneto-strictive transducer between high and low stiffness states. Switching is accomplished by either changing the applied magnetic field with a voltage excitation or changing the shunt impedance on the transducer's coil (i.e., the magneto-strictive material's magnetic boundary condition). Switched-stiffness vibration control is simulated using a lumped mass supported by a damper and the magneto-strictive transducer (mount), which is represented by a nonlinear, electromechanical model. Free vibration of the mass is calculated while varying the mount's stiffness according to a reference switched-stiffness vibration control law. The results reveal that switching the magnetic field produces the desired change in stiffness, but also an undesired actuation force that can significantly degrade the vibration control. Hence, a modified switched-stiffness control law that accounts for the actuation force is proposed and implemented for voltage-controlled stiffness switching. The influence of the magneto-mechanical bias condition is also discussed. Voltage-controlled stiffness switching is found to introduce damping equivalent to a viscous damping factor up to about 0.13; this is shown to primarily result from active vibration reduction caused by the actuation force. The merit of magneto-strictive switched-stiffness vibration control is then quantified by comparing the results of voltage- and shunt-controlled stiffness switching to the performance of optimal magneto-strictive shunt damping. For the cases considered, optimal resistive shunt damping performed considerably better than both voltage- and shunt-controlled stiffness switching.

  18. Hot pressed piezo-electric ceramic elements for ultrasonic transducers

    International Nuclear Information System (INIS)

    Piezoelectric ceramics (PZT4, PZT5, PZT7 and SPN) were investigated for development of high frequency (30-100 MHz) normal and focussed transducers. Hot pressed piezoelectric materials with a controlled grain size of ∼ 2 μm and approaching theoretical density can be lapped down to 30 μm thickness without structural damage whereas conventional sintered piezoelectric material with gram sizes ranging from 2-15 μm and at least 3% porosity can only be lapped down to 100 μm. Hot pressed piezoelectric ceramics when compared with conventional piezoelectric ceramics, show higher values of coupling coefficient (10-20%), elastic compliance ∼ 12% and moderate increases in mechanical quality factor (Qm) and dielectric constant. The compressional sound velocity measured along the poling direction is very sensitive to the switching of dipoles other than 180o ones. The velocity increases as the polarization increases and this constitutes a useful method for quality control of the piezoelectric ceramic as it gives the correct thickness resonant frequency. By accurately measuring the change by a comparison method, the dipole behaviour can be better understood and the degree of polarization and depolarization can be established. Temperature dependent polarization and depolarization is also discussed in the light of dipole switching. Hot pressed SPN and PZT5 are sufficiently transparent to be considered for use in opto-ultrasonic applications such as in medicine. Because of the high value of the thickness-frequency constant and low Qm, SPN seems a better choice as a high frequency transducer material. The signal spectra of a high frequency transducer constructed from these materials is presented

  19. Local Area Damage Detection in Composite Structures Using Piezoelectric Transducers

    CERN Document Server

    Lichtenwalner, Peter F

    1998-01-01

    An integrated and automated smart structures approach for structural health monitoring is presented, utilizing an array of piezoelectric transducers attached to or embedded within the structure for both actuation and sensing. The system actively interrogates the structure via broadband excitation of multiple actuators across a desired frequency range. The structure's vibration signature is then characterized by computing the transfer functions between each actuator/sensor pair, and compared to the baseline signature. Experimental results applying the system to local area damage detection in a MD Explorer rotorcraft composite flexbeam are presented.

  20. Quantum acousto-optic transducer for superconducting qubits

    CERN Document Server

    Shumeiko, V S

    2015-01-01

    We propose theory for reversible quantum transducer connecting superconducting qubits and optical photons using acoustic waves in piezoelectrics. The proposed device consists of integrated acousto-optic resonator that utilizes stimulated Brillouin scattering for phonon-photon conversion, and piezoelectric e?ect for coupling of phonons to qubits. We evaluate the phonon-photon coupling rate, and show that the required power of optical pump as well as the other device parameters providing full and faithful quantum conversion are feasible for implementation with the state of the art integrated acousto-optics.

  1. Speech recognition algorithms based on weighted finite-state transducers

    CERN Document Server

    Hori, Takaaki

    2013-01-01

    This book introduces the theory, algorithms, and implementation techniques for efficient decoding in speech recognition mainly focusing on the Weighted Finite-State Transducer (WFST) approach. The decoding process for speech recognition is viewed as a search problem whose goal is to find a sequence of words that best matches an input speech signal. Since this process becomes computationally more expensive as the system vocabulary size increases, research has long been devoted to reducing the computational cost. Recently, the WFST approach has become an important state-of-the-art speech recogni

  2. On the general governing equations of electromagnetic acoustic transducers

    CERN Document Server

    Saxena, Prashant

    2013-01-01

    In this paper, we present the general governing equations of electrodynamics and continuum mechanics that need to be considered while mathematically modelling the behaviour of electromagnetic acoustic transducers (EMATs). We consider the existence of finite deformations for soft materials and the possibility of electric currents, temperature gradients, and internal heat generation due to dissipation. Starting with Maxwell's equations of electromagnetism and balance laws of nonlinear elasticity, we present the governing equations and boundary conditions in incremental form in order to solve wave propagation problems of boundary value type.

  3. A distributed transducer system for functional electrical stimulation

    DEFF Research Database (Denmark)

    Gudnason, Gunnar; Nielsen, Jannik Hammel; Bruun, Erik;

    2001-01-01

    Implanted transducers for functional electrical stimulation (FES) powered by inductive links are subject to conflicting requirements arising from low link efficiency, a low power budget and the need for protection of the weak signals against strong RF electromagnetic fields. We propose a solution...... likely to be affected by the inductive link. Neural stimulators are affected to a lesser degree, but still benefit from the partitioning. As a test case, we have designed a transceiver and a sensor chip which implement this partitioning policy. The transceiver is designed to operate in the 6.78 MHz ISM...

  4. Development of a transducer for MiniGrail

    CERN Document Server

    Gottardi, L; Frossati, G

    2002-01-01

    We are developing a two-mode inductive resonant transducer for MiniGrail. We report several quality factor measurements, down to 4.2 K, performed on a scaled size resonator in different conditions: when suspended from a wire and when clamped, by thermal contraction techniques, into a hole of a sphere of 150 mm diameter and 14 kg mass. Q-factor measurements of a first resonator prototype at 4.2 K for MiniGrail are also presented. Finally, a fabrication process for a Nb film pick-up coil is described.

  5. Excitability of guided waves in composites with PWAS transducers

    Science.gov (United States)

    Shen, Yanfeng; Giurgiutiu, Victor

    2015-03-01

    Piezoelectric Wafer Active Sensors (PWAS) are convenient enablers for generating and receiving ultrasonic guided waves. The wide application of composite structures has put new challenges for the Structural Health Monitoring (SHM) and Nondestructive Evaluation (NDE) community due to the general anisotropic behaviors and complicated guided wave features in composites. The excitability of guided waves in composite structures directly influences the implementation of active sensing systems to achieve the best interrogation of certain sensing directions. This paper presents a hybrid modeling technique for studying the excitably of guided waves in composite structures with PWAS transducers. This hybrid technique comprehensively covers local finite element model (FEM), semi-analytical finite element (SAFE) method, and analytical guided wave solutions. Harmonic analysis of a small-size local FEM with non-reflective boundaries (NRB) was carried out for obtaining guided wave generation features in plate structures. The PWAS transducers were modeled with coupled filed elements. Thus, the FEM can fully capture the geometry and material property effects of PWAS transducers and their influence on the guided wave excitation. SAFE method was used to obtain the complicated guided wave features in composites such as dispersion curves and modeshapes. The SAFE procedure was coded into MATLAB Graphical User Interface (GUI), and the software SAFE-DISPERSION was developed. To study the excitability of each wave mode, we considered all the possible wave modes being generated simultaneously and propagating independently. The analytical wave expressions based on the exact guided wave solution with Hankel functions were used to join the SAFE method and the local FEM. Formulated in frequency domain, the hybrid model is highly efficient, providing an over determined equation system for the calculation of mode participation factors. Case studies were carried out: (1) the Lamb wave excitability

  6. Shaft transducer having dc output proportional to angular velocity

    Science.gov (United States)

    Handlykken, M. B. (Inventor)

    1984-01-01

    A brushless dc tachometer is disclosed that includes a high strength toroidal permanent magnet for providing a uniform magnetic field in an air gap, an annular pole piece opposite the magnet, and a pickup coil wound around the pole piece and adapted to rotate about the axis of the pole piece. The pickup coil is rotated by an input shaft to which the coil is coupled with the friction clip. The output of the coil is conducted to circuitry by a twisted wire pair. The input shaft also activates a position transducing potentiometer.

  7. Differential Pressure Transducer for Corrosion Monitoring of Iron

    Directory of Open Access Journals (Sweden)

    Amar Prasad Yadav

    2014-03-01

    Full Text Available In this study, differential pressure transducer (DPT has been applied as an alternate corrosion monitoring device for monitoring corrosion of iron in atmospheric environment by measuring very small changes in the amount of oxygen. The result of corrosion current obtained from DPT method has been compared with that obtained from AC impedance method. The difference in the value of corrosion current obtained from these two methods was attributed to the error in choosing the value of proportionality constant k of the Stern-Geary equation.

  8. Class D audio amplifiers for high voltage capacitive transducers

    DEFF Research Database (Denmark)

    Nielsen, Dennis

    with the low level of acoustical output power and complex amplifier requirements, have limited the commercial success of the technology. Horn or compression drivers are typically favoured, when high acoustic output power is required, this is however at the expense of significant distortion combined......, the DEAP (Dielectric ElectroActive Polymer) one. DEAP based loudspeakers work on the principle of the electrostatic forces, and possess some of the ii same characteristics as the electrostatic loudspeaker. However, the DEAP transducer is constructed by printing compliant, corrugated electrodes...

  9. Regulation and function of signal transducer and activator of transcription 3

    Institute of Scientific and Technical Information of China (English)

    Qian-Rong; Qi; Zeng-Ming; Yang

    2014-01-01

    Signal transducer and activator of transcription 3(STAT3), a member of the STAT family, is a key regulator of many physiological and pathological processes. Significant progress has been made in understanding the transcriptional control, posttranslational modification, cellular localization and functional regulation of STAT3. STAT3 can translocate into the nucleus and bind to specific promoter sequences, thereby exerting transcriptional regulation. Recent studies have shown that STAT3 can also translocate into mitochondria, participating in aerobic respiration and apoptosis. In addition, STAT3 plays an important role in inflammation and tumorigenesis by regulating cell proliferation, differentiation and metabolism. Conditional knockout mouse models make it possible to study the physiological function of STAT3 in specific tissues and organs. This review summarizes the latest advances in the understanding of the expression, regulation and function of STAT3 in physiological and tumorigenic processes.

  10. A Heme Oxygenase-1 Transducer Model of Degenerative and Developmental Brain Disorders

    Directory of Open Access Journals (Sweden)

    Hyman M. Schipper

    2015-03-01

    Full Text Available Heme oxygenase-1 (HO-1 is a 32 kDa protein which catalyzes the breakdown of heme to free iron, carbon monoxide and biliverdin. The Hmox1 promoter contains numerous consensus sequences that render the gene exquisitely sensitive to induction by diverse pro-oxidant and inflammatory stimuli. In “stressed” astroglia, HO-1 hyperactivity promotes mitochondrial iron sequestration and macroautophagy and may thereby contribute to the pathological iron deposition and bioenergetic failure documented in Alzheimer disease, Parkinson disease and certain neurodevelopmental conditions. Glial HO-1 expression may also impact neuroplasticity and cell survival by modulating brain sterol metabolism and the proteasomal degradation of neurotoxic proteins. The glial HO-1 response may represent a pivotal transducer of noxious environmental and endogenous stressors into patterns of neural damage and repair characteristic of many human degenerative and developmental CNS disorders.

  11. Design theory and experimental investigation of the low frequency and high power rare earth magnetostrictive flextensional transducer (I). Theoretical part

    Institute of Scientific and Technical Information of China (English)

    HE Xiping; SUN Jincai; LI Bin

    2001-01-01

    The energy relationships among all the elements, by which the magnetostrictive transducers are manufactured, in Finite Element Method (FEM) are analyzed, then the expressions of FEM dynamics equations and performances formulas for magnetostrictive transducers are derived. The vibrating modes of the class VII transducer and its shell vibration are calculated theoretically and the results point out that there is a breathing mode and if the transducer works at this mode, the transducer will vibrate with a greater volume speed and source level.

  12. Performance of transducers with segmented piezoelectric stacks using materials with high electromechanical coupling coefficient

    CERN Document Server

    Thompson, Stephen C; Markley, Douglas C

    2013-01-01

    Underwater acoustic transducers often include a stack of thickness polarized piezoelectric material pieces of alternating polarity interspersed with electrodes, bonded together and electrically connected in parallel. The stack is normally much shorter than a quarter wavelength at the fundamental resonance frequency, so that the mechanical behavior of the transducer is not affected by the segmentation. When the transducer bandwidth is less than a half octave, as has conventionally been the case, stack segmentation has no significant effect on the mechanical behavior of the device. However, when a high coupling coefficient material such as PMN-PT is used to achieve a wider bandwidth, the difference between a segmented stack and a similar piezoelectric section with electrodes only at the two ends can be significant. This paper investigates the effects of stack segmentation on the performance of wideband underwater acoustic transducers, particularly tonpilz transducer elements. Included is discussion of transduce...

  13. Variances and covariances in the Central Limit Theorem for the output of a transducer

    Science.gov (United States)

    Heuberger, Clemens; Kropf, Sara; Wagner, Stephan

    2015-01-01

    We study the joint distribution of the input sum and the output sum of a deterministic transducer. Here, the input of this finite-state machine is a uniformly distributed random sequence. We give a simple combinatorial characterization of transducers for which the output sum has bounded variance, and we also provide algebraic and combinatorial characterizations of transducers for which the covariance of input and output sum is bounded, so that the two are asymptotically independent. Our results are illustrated by several examples, such as transducers that count specific blocks in the binary expansion, the transducer that computes the Gray code, or the transducer that computes the Hamming weight of the width-w non-adjacent form digit expansion. The latter two turn out to be examples of asymptotic independence. PMID:27087727

  14. High frequency PMN-PT single crystal focusing transducer fabricated by a mechanical dimpling technique.

    Science.gov (United States)

    Chen, Y; Lam, K H; Zhou, D; Cheng, W F; Dai, J Y; Luo, H S; Chan, H L W

    2013-02-01

    High frequency (∼30MHz and ∼80MHz) focusing ultrasound transducers were fabricated using a PMN-0.28PT single crystal by a mechanical dimpling technique. The dimpled single crystal was used as an active element for the focusing transducer. Compared with a plane transducer, the focusing transducer fabricated with a dimpled active element exhibits much broader bandwidth and higher sensitivity. Besides, a high quality image can be obtained by the 30MHz focusing transducer, in which the -6dB axial and lateral resolution is 27μm and 139μm, respectively. These results prove that the dimpling technique is capable to fabricate the high frequency focusing transducers with excellent performance for imaging applications. PMID:22944074

  15. Use of Macro Fibre Composite Transducers as Acoustic Emission Sensors

    Directory of Open Access Journals (Sweden)

    Mark Eaton

    2009-04-01

    Full Text Available The need for ever lighter and more efficient aerospace structures and components has led to continuous optimization pushing the limits of structural performance. In order to ensure continued safe operation during long term service it is desirable to develop a structural health monitoring (SHM system. Acoustic emission (AE offers great potential for real time global monitoring of aerospace structures, however currently available commercial sensors have limitations in size, weight and adaptability to complex structures. This work investigates the potential use of macro-fibre composite (MFC film transducers as AE sensors. Due to the inhomogeneous make-up of MFC transducers their directional dependency was examined and found to have limited effect on signal feature data. However, signal cross-correlations revealed a strong directional dependency. The sensitivity and signal attenuation with distance of MFC sensors were compared with those of commercially available sensors. Although noticeably less sensitive than the commercial sensors, the MFC sensors still had an acceptable operating range. Furthermore, a series of compressive carbon fiber coupon tests were monitored in parallel using both an MFC sensor and a commercially available sensor for comparison. The results showed good agreement of AE trends recorded by both sensors.

  16. Transducer Development and Characterization for Underwater Acoustic Neutrino Detection Calibration

    Science.gov (United States)

    Saldaña, María; Llorens, Carlos D.; Felis, Ivan; Martínez-Mora, Juan Antonio; Ardid, Miguel

    2016-01-01

    A short bipolar pressure pulse with “pancake” directivity is produced and propagated when an Ultra-High Energy (UHE) neutrino interacts with a nucleus in water. Nowadays, acoustic sensor networks are being deployed in deep seas to detect this phenomenon as a first step toward building a neutrino telescope. In order to study the feasibility of the method, it is critical to have a calibrator that is able to mimic the neutrino signature. In previous works the possibility of using the acoustic parametric technique for this aim was proven. In this study, the array is operated at a high frequency and, by means of the parametric effect, the emission of the low-frequency acoustic bipolar pulse is generated mimicking the UHE neutrino acoustic pulse. To this end, the development of the transducer to be used in the parametric array is described in all its phases. The transducer design process, the characterization tests for the bare piezoelectric ceramic, and the addition of backing and matching layers are presented. The efficiencies and directivity patterns obtained for both primary and parametric beams confirm that the design of the proposed calibrator meets all the requirements for the emitter. PMID:27490547

  17. Transducer Development and Characterization for Underwater Acoustic Neutrino Detection Calibration.

    Science.gov (United States)

    Saldaña, María; Llorens, Carlos D; Felis, Ivan; Martínez-Mora, Juan Antonio; Ardid, Miguel

    2016-01-01

    A short bipolar pressure pulse with "pancake" directivity is produced and propagated when an Ultra-High Energy (UHE) neutrino interacts with a nucleus in water. Nowadays, acoustic sensor networks are being deployed in deep seas to detect this phenomenon as a first step toward building a neutrino telescope. In order to study the feasibility of the method, it is critical to have a calibrator that is able to mimic the neutrino signature. In previous works the possibility of using the acoustic parametric technique for this aim was proven. In this study, the array is operated at a high frequency and, by means of the parametric effect, the emission of the low-frequency acoustic bipolar pulse is generated mimicking the UHE neutrino acoustic pulse. To this end, the development of the transducer to be used in the parametric array is described in all its phases. The transducer design process, the characterization tests for the bare piezoelectric ceramic, and the addition of backing and matching layers are presented. The efficiencies and directivity patterns obtained for both primary and parametric beams confirm that the design of the proposed calibrator meets all the requirements for the emitter. PMID:27490547

  18. Encapsulation of Capacitive Micromachined Ultrasonic Transducers Using Viscoelastic Polymer.

    Science.gov (United States)

    Lin, Der-Song; Zhuang, Xuefeng; Wong, Serena H; Kupnik, Mario; Khuri-Yakub, Butrus Thomas

    2010-12-01

    The packaging of a medical imaging or therapeutic ultrasound transducer should provide protective insulation while maintaining high performance. For a capacitive micromachined ultrasonic transducer (CMUT), an ideal encapsulation coating would therefore require a limited and predictable change on the static operation point and the dynamic performance, while insulating the high dc and dc actuation voltages from the environment. To fulfill these requirements, viscoelastic materials, such as polydimethylsiloxane (PDMS), were investigated for an encapsulation material. In addition, PDMS, with a glass-transition temperature below room temperature, provides a low Young's modulus that preserves the static behavior; at higher frequencies for ultrasonic operation, this material becomes stiffer and acoustically matches to water. In this paper, we demonstrate the modeling and implementation of the viscoelastic polymer as the encapsulation material. We introduce a finite element model (FEM) that addresses viscoelasticity. This enables us to correctly calculate both the static operation point and the dynamic behavior of the CMUT. CMUTs designed for medical imaging and therapeutic ultrasound were fabricated and encapsulated. Static and dynamic measurements were used to verify the FEM and show excellent agreement. This paper will help in the design process for optimizing the static and the dynamic behavior of viscoelastic-polymer-coated CMUTs. PMID:21170294

  19. Nonlinear electromechanical response of the ferroelectret ultrasonic transducers

    Science.gov (United States)

    Döring, Joachim; Bovtun, Viktor; Bartusch, Jürgen; Erhard, Anton; Kreutzbruck, Marc; Yakymenko, Yuriy

    2010-08-01

    The ultrasonic transmission between two air-coupled polypropylene (PP) ferroelectret (FE) transducers in dependence on the amplitude of the high-voltage exciting pulse revealed a strongly nonlinear electromechanical response of the FE transmitter. This phenomenon is described by a linear increase of the inverse electromechanical transducer constant t_{33}^{(1)} of the PP FE film with an increase of the exciting electrical pulse amplitude. Enlargement of t_{33}^{(1)} by a factor of 4 was achieved by application of 3500 V exciting pulses. The electrostriction contribution to t_{33}^{(1)} can be attributed to the electrostatic force between electrodes and the Maxwell stress effect. The nonlinear electromechanical properties of the PP FE result in a strong increase of its air-coupled ultrasonic (ACUS) figure of merit ( FOM) under the high-voltage excitation, which exceeds results of the PP FE technological optimization. The FOM increase can be related to the increase of PP FE coupling factor and/or to the decrease of its acoustic impedance. A significant enhancement of the ACUS system transmission (12 dB) and signal-to-noise ratio (32 dB) was demonstrated by the increase of excitation voltage up to 3500 V. The nonlinear electromechanical properties of the PP FEs seem to be very important for their future applications.

  20. Transducer Development and Characterization for Underwater Acoustic Neutrino Detection Calibration.

    Science.gov (United States)

    Saldaña, María; Llorens, Carlos D; Felis, Ivan; Martínez-Mora, Juan Antonio; Ardid, Miguel

    2016-01-01

    A short bipolar pressure pulse with "pancake" directivity is produced and propagated when an Ultra-High Energy (UHE) neutrino interacts with a nucleus in water. Nowadays, acoustic sensor networks are being deployed in deep seas to detect this phenomenon as a first step toward building a neutrino telescope. In order to study the feasibility of the method, it is critical to have a calibrator that is able to mimic the neutrino signature. In previous works the possibility of using the acoustic parametric technique for this aim was proven. In this study, the array is operated at a high frequency and, by means of the parametric effect, the emission of the low-frequency acoustic bipolar pulse is generated mimicking the UHE neutrino acoustic pulse. To this end, the development of the transducer to be used in the parametric array is described in all its phases. The transducer design process, the characterization tests for the bare piezoelectric ceramic, and the addition of backing and matching layers are presented. The efficiencies and directivity patterns obtained for both primary and parametric beams confirm that the design of the proposed calibrator meets all the requirements for the emitter.

  1. Numerical modeling of piezoelectric transducers using physical parameters.

    Science.gov (United States)

    Cappon, Hans; Keesman, Karel J

    2012-05-01

    Design of ultrasonic equipment is frequently facilitated with numerical models. These numerical models, however, need a calibration step, because usually not all characteristics of the materials used are known. Characterization of material properties combined with numerical simulations and experimental data can be used to acquire valid estimates of the material parameters. In our design application, a finite element (FE) model of an ultrasonic particle separator, driven by an ultrasonic transducer in thickness mode, is required. A limited set of material parameters for the piezoelectric transducer were obtained from the manufacturer, thus preserving prior physical knowledge to a large extent. The remaining unknown parameters were estimated from impedance analysis with a simple experimental setup combined with a numerical optimization routine using 2-D and 3-D FE models. Thus, a full set of physically interpretable material parameters was obtained for our specific purpose. The approach provides adequate accuracy of the estimates of the material parameters, near 1%. These parameter estimates will subsequently be applied in future design simulations, without the need to go through an entire series of characterization experiments. Finally, a sensitivity study showed that small variations of 1% in the main parameters caused changes near 1% in the eigenfrequency, but changes up to 7% in the admittance peak, thus influencing the efficiency of the system. Temperature will already cause these small variations in response; thus, a frequency control unit is required when actually manufacturing an efficient ultrasonic separation system.

  2. High-resolution diffraction grating interferometric transducer of linear displacements

    Science.gov (United States)

    Shang, Ping; Xia, Haojie; Fei, Yetai

    2016-01-01

    A high-resolution transducer of linear displacements is presented. The system is based on semiconductor laser illumination and a diffraction grating applied as a length master. The theory of the optical method is formulated using Doppler description. The relationship model among the interference strips, measurement errors, grating deflection around the X, Y and Z axes and translation along the Z axis is built. The grating interference strips' direction and space is not changed with movement along the X (direction of grating movement), Y (direction of grating line), Z axis, and the direction and space has a great effect when rotating around the X axis. Moreover the space is little affected by deflection around the Z axis however the direction is changed dramatically. In addition, the strips' position shifted rightward or downwards respectively for deflection around the X or Y axis. Because the emitted beams are separated on the grating plane, the tilt around the X axis error of the stage during motion will lead to the optical path difference of the two beams resulting in phase shift. This study investigates the influence of the tilt around the X axis error. Experiments show that after yaw error compensation, the high-resolution diffraction grating interferometric transducer readings can be significantly improved. The error can be reduced from +/-80 nm to +/-30 nm in maximum.

  3. A stable, sensitive, low-compliance capacitance force transducer.

    Science.gov (United States)

    Hamrell, B B; Panaanan, R; Trono, J; Alpert, N R

    1975-01-01

    The measurement of active and passive force levels in heart muscle requires short-and long-term base-line stability. The capacitance force transducer described here represents an optimization of the relationship between sensitivity, compliance, and frequency response in a design that minimizes long-term base-line drift related to thermal gradients within the apparatus. Thermal stability of the instrument is obtained with the use of quartz and Invar in the construction of the variable capacitor, the maintenance of internal transducer temperature at a constant level well above ambient, and the use of thermally insulating air gaps. Sensitivity ranges from 1.0 to 2.0 V/g wt in the several instruments tested, the output is linear, compliance is negligible with static loads up to 6 g wt, hysteresis is not significant with transient loading with 20 g wt, and long-term drift is greater than or equal to 0.050 g wt. These instruments are designed for use with myocardial preparations but can be adapted for skeletal muscle experiments. PMID:1110237

  4. Analysis of eigenfrequencies in piezoelectric transducers using the finite element method

    DEFF Research Database (Denmark)

    Jensen, Henrik

    1988-01-01

    It is noted that the finite-element method is a valuable supplement to the traditional methods for design of novel transducer types because it can determine the vibrational pattern of piezoelectric transducers and is applicable to any geometry. Computer programs for analysis of axisymmetric...... in an efficient way. Examples ranging from a freely vibrating disk to a real focused transducer with a conical backing are presented...

  5. Techniques and physical properties of 10MHz short pulse focused ultrasonic transducer

    Institute of Scientific and Technical Information of China (English)

    ZHU Guozhen; YANG Yong; LU Kean

    2004-01-01

    A focused ultrasonic transducer used for biomedical purposes with a fundamental frequency of 10MHz and a pulse width of one and a half periods is described in this paper. Its physical properties are given including (1) focused acoustic field recorded by an optical means, (2) electric waveform for triggering the transducer and the corresponding waveform of the wave received by another transducer, and (3) result of tests on a sample object.

  6. Advantage analysis of PMN-PT material for free-flooded ring transducers

    Institute of Scientific and Technical Information of China (English)

    He Zheng-Yao; Ma Yuan-Liang

    2011-01-01

    The acoustic radiation characteristics of free-flooded ring transducers made of PZT4 and PMN-PT materials are calculated and compared.First,the theoretical formulae for free-flooded ring transducers are studied.The resonant frequencies of a transducer made of PZT4 and PMN-PT materials are calculated. Then,the transmitting voltage responses of the free-flooded ring transducers are calculated using the finite element method.Finally,the acoustic radiation characteristics of the free-flooded ring transducers are calculated using the boundary element method.The calculated results show that the resonant frequencies of the free-flooded ring transducer made of PMN-PT are greatly reduced compared with those made of PZT4 with the same size.The transmitting voltage response of the transducer made of PMN-PT is much higher than that of the transducer made of PZT4.The calculated 3-dB beamwidth of the acoustic radiated far-field directivity of the free-flooded ring transducer made of PZT4 at the resonant frequency 1900 Hz is 63.6° and that of the transducer made of PMN-PT at the resonant frequency 1000 Hz is 64.6°.The comparison results show that the free-flooded ring transducer made of PMN-PT material has many advantages over that made of PZT4.The PMN-PT is a promising material for improving the performance of free-flooded ring transducers.

  7. Technology trends in high temperature pressure transducers: The impact of micromachining

    Science.gov (United States)

    Mallon, Joseph R., Jr.

    1992-01-01

    This paper discusses the implications of micromachining technology on the development of high temperature pressure transducers. The introduction puts forth the thesis that micromachining will be the technology of choice for the next generation of extended temperature range pressure transducers. The term micromachining is defined, the technology is discussed and examples are presented. Several technologies for high temperature pressure transducers are discussed, including silicon on insulator, capacitive, optical, and vibrating element. Specific conclusions are presented along with recommendations for development of the technology.

  8. Temperature and pressure transducer based on FBG for large diameter water pipes

    OpenAIRE

    Quintela Incera, Antonio; Pallol Pérez, María José; Shokry Girgis Roufael, Hany; Martínez García, Óscar; San Emeterio Diego, José Domingo; López Higuera, José MIguel

    2014-01-01

    A specific temperature and pressure optical fiber transducer is presented in this paper. By using a customized fiber reinforced plastic membrane with embedded Fiber Bragg Gratings, the fluid pressure and temperatures changes are converted in optical wavelength displacements. The membrane and the transducer custom design allows a suitable measurand discrimination. The transducer is implemented, characterized and calibrated. Its feasibility to be used on large diameter water pipes has been succ...

  9. Cryogenic Clamp-on Ultrasonic Flowmeters using Single Crystal Piezoelectric Transducers Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Clamp-on ultrasound cryogenic flowmeters using single crystal piezoelectric transducers are proposed to enable reliable, accurate cryogenic instrumentation needs in...

  10. An absolute method for determination of misalignment of an immersion ultrasonic transducer.

    Science.gov (United States)

    Narayanan, M M; Singh, Narender; Kumar, Anish; Babu Rao, C; Jayakumar, T

    2014-12-01

    An absolute methodology has been developed for quantification of misalignment of an ultrasonic transducer using a corner-cube retroreflector. The amplitude based and the time of flight (TOF) based C-scans of the reflector are obtained for various misalignments of the transducer. At zero degree orientation of the transducer, the vertical positions of the maximum amplitude and the minimum TOF in the C-scan coincide. At any other orientation of the transducer with the horizontal plane, there is a vertical shift in the position of the maximum amplitude with respect to the minimum TOF. The position of the minimum (TOF) remains the same irrespective of the orientation of the transducer and hence is used as a reference for any misalignment of the transducer. With the measurement of the vertical shift and the horizontal distance between the transducer and the vertex of the reflector, the misalignment of the transducer is quantified. Based on the methodology developed in the present study, retroreflectors are placed in the Indian 500MWe Prototype Fast Breeder Reactor for assessment of the orientation of the ultrasonic transducer prior to the under-sodium ultrasonic scanning for detection of any protrusion of the subassemblies. PMID:25041979

  11. New improvement of the combined optical fiber transducer for landslide monitoring

    Science.gov (United States)

    Zhu, Z.-W.; Yuan, Q.-Y.; Liu, D.-Y.; Liu, B.; Liu, J.-C.; Luo, H.

    2014-08-01

    Landslide monitoring is important in predicting the behavior of landslides, thereby ensuring environmental, life, and property safety. On the basis of our previous studies, we conducted the double shear test by using a third-generation optical fiber transducer that uses expandable polystyrene (EPS) as base material. However, the third-generation transducer has poor performance when cohesive force is present between the grout and capillary stainless steel pipe of the transducer. Thus, the fourth-generation optical fiber transducer was invented. Similar to the third-generation transducer, the fourth-generation transducer also used EPS as its base material. Single shear test was conducted on the fourth-generation transducer after being grouted with cement mortar (1 : 1 mix ratio). The micro-bend loss mechanism of the optical fiber was considered, and the optical time domain reflectometry instrument was used. The fact that the loss sequence of optical fibers subjected to loading is different at various locations is found. The relationship of the loading-point displacement vs. optical fiber sliding distance and optical loss were measured. Results show that the maximum initial measurement precision of the newly proposed device is 1 mm, the corresponding sliding distance is 21 mm, and the dynamic range is 0-20 mm. The fourth-generation transducer can measure the movement direction of loadings, thus making this transducer applicable for landslide monitoring.

  12. Principle of adaptive optical current transducer on independent variables and its test

    Institute of Scientific and Technical Information of China (English)

    LU Zhong-feng; GUO Zhi-zhong

    2008-01-01

    In order to improve the measurement precision of the optical current transducer (OCT), the adaptive optical transducing principle on independent variables is presented in this paper. And one of the adaptive opti-cal current transducer(AOCT) on the independent variables of the output of the electricmegnet current trans-ducer is introduced. According to IEC660044-8, the performance of AOCT was examined roundly applying the standard testing system authenticated by the state authority. The results indicate that the measurement precision of the AOCT has already reached 0.2 class under the temperature from -40℃ to 60℃,which proves the feasi-bility of the method.

  13. Tunable-angle wedge transducer for improved acoustophoretic control in a microfluidic chip

    DEFF Research Database (Denmark)

    Iranmanesh, I.; Barnkob, Rune; Bruus, Henrik;

    2012-01-01

    coupling angle, and transducer actuation method (single-frequency actuation or frequency-modulation actuation). The energy-density analysis is based on measuring the transmitted light intensity through a microfluidic channel filled with a suspension of 5-μm-diameter beads and the results with the tunable...... particle patterns with average acoustic energy densities comparable to those obtained using single-frequency actuation.......We present a tunable-angle wedge ultrasound transducer for improved control of microparticle acoustophoresis in a microfluidic chip. The transducer is investigated by analyzing the pattern of aligned particles and induced acoustic energy density while varying the system geometry, transducer...

  14. The influence of roughness, angle, range, and transducer type on the echo signal from planar interfaces

    DEFF Research Database (Denmark)

    Wilhjelm, Jens E.; Pedersen, Peter C.; Jacobsen, Søren Mehl

    2001-01-01

    The received electrical echo signal from a pulse-echo system insonifying a planar interface was measured for varying degrees of rms roughness [0 to 0.29 mm (0 to 1.7 /spl lambda/)], angles of incidence, /spl theta/, (-7/spl deg/ to 7/spl deg/), and ranges to a planar or focused transducer......B/(Rq//spl lambda/) for planar and focused transducers, respectively. The characteristic nulls present in the normalized spectra of the echo signal at non-normal incidence tend to vanish with increasing R/sub q/ when using planar transducers. For focused transducers, the normalized spectra change from relatively...

  15. The design and calibration of particular geometry piezoelectric acoustic emission transducer for leak detection and localization

    Science.gov (United States)

    Yalcinkaya, Hazim; Ozevin, Didem

    2013-09-01

    Pipeline leak detection using an acoustic emission (AE) method requires highly sensitive transducers responding to less attenuative and dispersive wave motion in order to place the discrete transducer spacing in an acceptable approach. In this paper, a new piezoelectric transducer geometry made of PZT-5A is introduced to increase the transducer sensitivity to the tangential direction. The finite element analysis of the transducer geometry is modeled in the frequency domain to identify the resonant frequency, targeting 60 kHz, and the loss factor. The numerical results are compared with the electromechanical characterization tests. The transducer response to wave motion generated in different directions is studied using a multiphysics model that couples mechanical and electrical responses of structural and piezoelectric properties. The directional dependence and the sensitivity of the transducer response are identified using the laser-induced load function. The transducer response is compared with a conventional thickness mode AE transducer under simulations and leak localization in a laboratory scale steel pipe.

  16. OPTIMAL DESIGN OF A 6-AXIS FORCE TRANSDUCER BASED ON STEWART PLATFORM RELATED TO SENSITIVITY ISOTROPY

    Institute of Scientific and Technical Information of China (English)

    2003-01-01

    The design method of a 6-axis force robot's transducer based on the Stewart platform is detailed.For this purpose, the sensitivity isotropy evaluation criteria of the transducer are defined, and by the aid of computer, the relationships between the criteria and the parameters of all the transducers based on the Stewart platform are investigated within the geometric model of the solution space, which can provide the theoretical background for the optimal construction design of the 6-axis force transducer related to the sensitivity isotropy.

  17. A High-Voltage Class D Audio Amplifier for Dielectric Elastomer Transducers

    DEFF Research Database (Denmark)

    Nielsen, Dennis; Knott, Arnold; Andersen, Michael A. E.

    2014-01-01

    Dielectric Elastomer (DE) transducers have emerged as a very interesting alternative to the traditional electrodynamic transducer. Lightweight, small size and high maneuverability are some of the key features of the DE transducer. An amplifier for the DE transducer suitable for audio applications...... is proposed and analyzed. The amplifier addresses the issue of a high impedance load, ensuring a linear response over the midrange region of the audio bandwidth (100 Hz – 3.5 kHz). THD+N below 0.1% are reported for the ± 300 V prototype amplifier producing a maximum of 125 Var at a peak efficiency of 95 %....

  18. Piezoelectric polymer foams: transducer mechanism and preparation as well as touch-sensor and ultrasonic-transducer properties

    Science.gov (United States)

    Wegener, M.

    2010-04-01

    Different materials provide a mechanical-electrical energy conversion and are thus interesting candidates for piezoelectric sensors and actuators. Beside ferroelectric ceramics and polymers, also polymer foams, so-called ferroelectrets, are developed as piezoelectric active materials. Their piezoelectricity originates from optimized structural and elastic-foam properties accompanied with an optimized charge trapping at the polymer layers within the foam structure. The piezoelectric activity arises if mechanical stimuli lead to a thickness variation of the electrically charged voids which results in an electrical signal between the connected electrodes on the film surfaces due to the change of internal electric fields. The concept of such a piezoelectric transducer was developed by investigating cellular polypropylene films with different foam structures and thus different elastic properties. Recently, ferroelectrets were prepared from other polymers following the same concept. Different kind of new foaming procedures are developed in order to broaden the range of usable materials as well as to optimize the adjustment of piezoelectric and ultrasonictransducer properties. The paper provides an overview about ferroelectrets, their underlying working mechanism as well as their preparation possibilities. In detail, piezoelectric properties of polypropylene ferroelectrets are described which are usable for pushbutton or touch-pad applications as well as in ultrasonic-transducer applications.

  19. Gene therapy of T helper cells in HIV infection: mathematical model of the criteria for clinical effect

    DEFF Research Database (Denmark)

    Lund, O; Lund, O S; Gram, G;

    1997-01-01

    This paper presents a mathematical analysis of the criteria for gene therapy of T helper cells to have a clinical effect on HIV infection. The analysis indicates that for such a therapy to be successful, it must protect the transduced cells against HIV-induced death. The transduced cells...... will not survive as a population if the gene therapy only blocks the spread of virus from transduced cells that become infected. The analysis also suggests that the degree of protection against disease-related cell death provided by the gene therapy is more important than the fraction cells that is initially...... transduced. If only a small fraction of the cells can be transduced, transduction of T helper cells and transduction of haematopoietic progenitor cells will result in the same steady-state level of transduced T helper cells. For gene therapy to be efficient against HIV infection, our analysis suggests...

  20. On-chip RF-to-optical transducer (Conference Presentation)

    Science.gov (United States)

    Simonsen, Anders; Tsaturyan, Yeghishe; Seis, Yannick; Schmid, Silvan; Schliesser, Albert; Polzik, Eugene S.

    2016-04-01

    techniques. We will furthermore present ongoing work to couple our transducer to an RF or microwave antenna, for low-noise detection of electromagnetic signals, including sensitive measurements of magnetic fields in an MRI detector. Suppression of thermomechanical noise is a key feature of electro-optomechanical transducers, and, more generally, hybrid systems involving mechanical degrees of freedom. We have shown that engineering of the phononic density of states allows improved isolation of the relevant mechanical modes from their thermal bath [2], enabling coherence times sufficient to realize quantum-coherent optomechanical coupling. This proves the potential of the employed platform for complex transducers all the way into the quantum regime. References: [1] Bagci et al, Nature 507, 81-85, (06 March 2014) [2] Tsaturyan, et al., Optics Express, Vol. 22, Issue 6, pp. 6810-6821 (2014)

  1. Feasibility test of NaK filled pressure transducers for sodium test facility

    Energy Technology Data Exchange (ETDEWEB)

    Choi, Youngil; Kim, Jong-Man; Ko, Yungju; Kim, Hyungmo; Lee, Dongwon; Cho, Chungho; Jung, Min-Hwan; Lee, Jewhan; Gam, Da-Young; Jeong, Ji-Young [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2015-10-15

    In this paper, feasibility tests of NaK filled pressure transduces in a relatively low pressure level were performed for a sodium test facility. In the test, model KE pressure transducers made by GEFRAN were tested in a pressure range (i.e., up to 200 kPa) which usually expected for a sodium test facility. The validation of using in a low pressure level was confirmed by the feasibility tests as well as high pressure. Most of the sodium experiments are performed at the high temperature condition. And on account of the strong chemical reactivity of sodium and solidification in a room temperature, many pressure transducers cannot be adapted directly. To use existing pressure transducers without any intermediate medium, it must be equipped by heaters, valves and safety devices to avoid solidification and to prevent chemical reaction of sodium. The pressure transducer filled NaK (Sodiumpotassium alloy is generally referred to as NaK.) is selected as pressure transducers for sodium test facility. The advantage of NaK over sodium is a lower melting point than a room temperature. The lower melting point can make a pressure transducer can be directly used without additional parts such as heaters and valves. NaK filled pressure transducer will be validated at high temperature and robustness in long term uses and repetition in our sodium test facility at KAERI. The lower melting point of NaK can make a pressure transducer simple without many devices such as heater, valve, leak detector and many electronic measuring points. In this paper, application of pressure transducers filled NaK (KE) to sodium test facility (SELFA) was described. In an expected pressure range, the linearity is still represented considerably. In the near future, the pressure transducer with NaK will be adapted to a sodium test facility.

  2. Direct mechanical stimulation of tip links in hair cells through DNA tethers

    Science.gov (United States)

    Basu, Aakash; Lagier, Samuel; Vologodskaia, Maria; Fabella, Brian A; Hudspeth, AJ

    2016-01-01

    Mechanoelectrical transduction by hair cells commences with hair-bundle deflection, which is postulated to tense filamentous tip links connected to transduction channels. Because direct mechanical stimulation of tip links has not been experimentally possible, this hypothesis has not been tested. We have engineered DNA tethers that link superparamagnetic beads to tip links and exert mechanical forces on the links when exposed to a magnetic-field gradient. By pulling directly on tip links of the bullfrog's sacculus we have evoked transduction currents from hair cells, confirming the hypothesis that tension in the tip links opens transduction channels. This demonstration of direct mechanical access to tip links additionally lays a foundation for experiments probing the mechanics of individual channels. DOI: http://dx.doi.org/10.7554/eLife.16041.001 PMID:27331611

  3. A novel chimeric adenoassociated virus 2/human bocavirus 1 parvovirus vector efficiently transduces human airway epithelia.

    Science.gov (United States)

    Yan, Ziying; Keiser, Nicholas W; Song, Yi; Deng, Xuefeng; Cheng, Fang; Qiu, Jianming; Engelhardt, John F

    2013-12-01

    Human bocavirus virus-1 (HBoV1), a newly discovered autonomous parvovirus with a 5,500 nt genome, efficiently infects human-polarized airway epithelia (HAE) from the apical membrane. We hypothesized that the larger genome and high airway tropism of HBoV1 would be ideal for creating a viral vector for lung gene therapy. To this end, we successfully generated recombinant HBoV1 (rHBoV1) from an open reading frames-disrupted rHBoV1 genome that efficiently transduces HAE from the apical surface. We next evaluated whether HBoV1 capsids could package oversized rAAV2 genomes. These studies created a rAAV2/HBoV1 chimeric virus (5.5 kb genome) capable of apically transducing HAE at 5.6- and 70-fold greater efficiency than rAAV1 or rAAV2 (4.7-kb genomes), respectively. Molecular studies demonstrated that viral uptake from the apical surface was significantly greater for rAAV2/HBoV1 than for rAAV2 or rAAV1, and that polarization of airway epithelial cells was required for HBoV1 capsid-mediated gene transfer. Furthermore, rAAV2/HBoV1-CFTR virus containing the full-length cystic fibrosis transmembrane conductance regulator (CFTR) gene coding sequence and the strong CBA promoter efficiently corrected CFTR-dependent chloride transport in cystic fibrosis (CF) HAE. In summary, using the combined advantages of AAV and HBoV1, we have developed a novel and promising viral vector for CF lung gene therapy and also potentially HBoV1 vaccine development. PMID:23896725

  4. A RF superconducting electromechanical transducer for gravitational wave antennae

    Science.gov (United States)

    Bocko, Mark F.; Johnson, Warren W.; Iafolla, Valerio

    1989-03-01

    An electromechanical transducer based on a superconducting radio-frequency bridge circuit has been developed for use on a gravitational radiation detector. The low electrical loss of superconductors has made it possible to achieve electrical quality factors of several thousand in a lumped-element circuit which operates at 4 MHz. The bridge could be remotely balanced to one part in 50,000, which led to a displacement noise level of 10-15 m/sq rt Hz. It should be useful in measuring any physical quantity which can be made to change a capacitance. At the present stage of development, capacitance changes of 10-20 F could be detected in a 1-s integration time. One straightforward improvement, namely, the use of a low-phase-noise quartz crystal oscillator as the bridge excitation source, will reduce the noise to 10-17 m/sq rt Hz.

  5. Optical detection of radio waves through a nanomechanical transducer

    CERN Document Server

    Bagci, T; Schmid, S; Villanueva, L G; Zeuthen, E; Appel, J; Taylor, J M; Sørensen, A; Usami, K; Schliesser, A; Polzik, E S

    2013-01-01

    Low-loss transmission and sensitive recovery of weak radio-frequency (rf) and microwave signals is an ubiquitous technological challenge, crucial in fields as diverse as radio astronomy, medical imaging, navigation and communication, including those of quantum states. Efficient upconversion of rf-signals to an optical carrier would allow transmitting them via optical fibers dramatically reducing losses, and give access to the mature toolbox of quantum optical techniques, routinely enabling quantum-limited signal detection. Research in the field of cavity optomechanics has shown that nanomechanical oscillators can couple very strongly to either microwave or optical fields. An oscillator accommodating both functionalities would bear great promise as the intermediate platform in a radio-to-optical transduction cascade. Here, we demonstrate such an opto-electro-mechanical transducer utilizing a high-Q nanomembrane. A moderate voltage bias (<10V) is sufficient to induce strong coupling between the voltage fluct...

  6. New Soft Polymeric Materials Applicable as Elastomeric Transducers

    DEFF Research Database (Denmark)

    Bejenariu, Anca Gabriela; Skov, Anne Ladegaard

    An elastomer is a material characterized by the capability to regain its original size and shape after being deformed (stretched or distorted). An ideal elastomer for electroactive polymer (EAP) applications is a system characterized by high extensibility, flexibility and a good mechanical fatigue...... between two compliant electrodes will reduce its thickness and expand its area. The electrical energy transformed into mechanical energy is called actuation and it is studied in the technology of elastomeric transducers. While DEs deform under high voltage, the actuation varies for different materials...... presents new soft polymeric materials based on silicone with improved mechanical properties. Silicone elastomers exhibit good characteristics including biocompatibility, oxidation resistance, thermal stability, fast mechanical response with good reproducibility and stable mechanical behaviour over a wide...

  7. Modeling of nonlinear responses for reciprocal transducers involving polarization switching

    DEFF Research Database (Denmark)

    Willatzen, Morten; Wang, Linxiang

    2007-01-01

    Nonlinearities and hysteresis effects in a reciprocal PZT transducer are examined by use of a dynamical mathematical model on the basis of phase-transition theory. In particular, we consider the perovskite piezoelectric ceramic in which the polarization process in the material can be modeled...... by Landau theory for the first-order phase transformation, in which each polarization state is associated with a minimum of the Landau free-energy function. Nonlinear constitutive laws are obtained by using thermodynamical equilibrium conditions, and hysteretic behavior of the material can be modeled...... intrinsically. The time-dependent Ginzburg-Landau theory is used in the parameter identification involving hysteresis effects. We use the Chebyshev collocation method in the numerical simulations. The elastic field is assumed to be coupled linearly with other fields, and the nonlinearity is in the E-D coupling...

  8. Optical detection of radio waves through a nanomechanical transducer

    DEFF Research Database (Denmark)

    Bagci, Tolga; Simonsen, A; Schmid, Silvan;

    2013-01-01

    Low-loss transmission and sensitive recovery of weak radio-frequency (rf) and microwave signals is an ubiquitous technological challenge, crucial in fields as diverse as radio astronomy, medical imaging, navigation and communication, including those of quantum states. Efficient upconversion of rf......] has shown that nanomechanical oscillators can couple very strongly to either microwave [3–5] or optical fields [6, 7]. An oscillator accommodating both these functionalities would bear great promise as the intermediate platform in a radio-to-optical transduction cascade. Here, we demonstrate such an...... opto-electro-mechanical transducer following a recent proposal [8] utilizing a high-Q nanomembrane. A moderate voltage bias (Vdc < 10V) is sufficient to induce strong coupling [4, 6, 7] between the voltage fluctuations in a radio-frequency resonance circuit and the membrane’s displacement, which is...

  9. Research on recognition of ramp angle based on transducer

    Directory of Open Access Journals (Sweden)

    Wenhao GU

    2015-12-01

    Full Text Available Focusing on the recognition of ramp angle, the relationship between the signal of vehicle transducer and real ramp angle is studied. The force change of vehicle on the ramp, and the relationship between the body tilt angle and front and rear suspension scale is discussed. According to the suspension and tire deformation, error angle of the ramp angle is deduced. A mathematical model is established with Matlab/Simulink and used for simulation to generate error curve of ramp angle. The results show that the error angle increases with the increasing of the ramp angle, and the limit value can reach 6.5%, while the identification method can effectively eliminate this error, and enhance the accuracy of ramp angle recognition.

  10. Non-destructive evaluation method employing dielectric electrostatic ultrasonic transducers

    Science.gov (United States)

    Yost, William T. (Inventor); Cantrell, Jr., John H. (Inventor)

    2003-01-01

    An acoustic nonlinearity parameter (.beta.) measurement method and system for Non-Destructive Evaluation (NDE) of materials and structural members novelly employs a loosely mounted dielectric electrostatic ultrasonic transducer (DEUT) to receive and convert ultrasonic energy into an electrical signal which can be analyzed to determine the .beta. of the test material. The dielectric material is ferroelectric with a high dielectric constant .di-elect cons.. A computer-controlled measurement system coupled to the DEUT contains an excitation signal generator section and a measurement and analysis section. As a result, the DEUT measures the absolute particle displacement amplitudes in test material, leading to derivation of the nonlinearity parameter (.beta.) without the costly, low field reliability methods of the prior art.

  11. Small, fast, and tough: Shrinking down integrated elastomer transducers

    Science.gov (United States)

    Rosset, Samuel; Shea, Herbert R.

    2016-09-01

    We review recent progress in miniaturized dielectric elastomer actuators (DEAs), sensors, and energy harvesters. We focus primarily on configurations where the large strain, high compliance, stretchability, and high level of integration offered by dielectric elastomer transducers provide significant advantages over other mm or μm-scale transduction technologies. We first present the most active application areas, including: tunable optics, soft robotics, haptics, micro fluidics, biomedical devices, and stretchable sensors. We then discuss the fabrication challenges related to miniaturization, such as thin membrane fabrication, precise patterning of compliant electrodes, and reliable batch fabrication of multilayer devices. We finally address the impact of miniaturization on strain, force, and driving voltage, as well as the important effect of boundary conditions on the performance of mm-scale DEAs.

  12. Signal processing for all fiber optical current transducer

    Institute of Scientific and Technical Information of China (English)

    裴焕斗; 祖静; 陈鸿

    2008-01-01

    The work principle of all fiber optical current transducer (AFOCT) was introduced. By analyzing the characteristic of photo-detector’s output, a measurement and signal processing scheme based on sine wave modulation and demodulation was put forward for eliminating the influence of light intensity change and modulation degree change. A digital signal processing system and a calibration scheme were also advanced. The experimental data show that the mean ratio error is 0.016 74% for direct current and 0.035% for alternating current, and the correlation coefficient of linearity is up to 0.999 982 4, meeting the precision requirement of 0.2 grade. Stability experiments and temperature drift experiments show the AFOCT has a better stable capability.

  13. Steerable vertical to horizontal energy transducer for mobile robots

    Energy Technology Data Exchange (ETDEWEB)

    Spletzer, Barry L. (Albuquerque, NM); Fischer, Gary J. (Albuquerque, NM); Feddema, John T. (Albuquerque, NM)

    2001-01-01

    The present invention provides a steerable vertical to horizontal energy transducer for mobile robots that less complex and requires less power than two degree of freedom tilt mechanisms. The present invention comprises an end effector that, when mounted with a hopping actuator, translates along axis (typically vertical) actuation into combined vertical and horizontal motion. The end effector, or foot, mounts with an end of the actuator that moves toward the support surface (typically a floor or the earth). The foot is shaped so that the first contact with the support surface is off the axis of the actuator. Off-axis contact with the support surface generates an on-axis force (typically resulting in vertical motion) and a moment orthogonal to the axis. The moment initiates a horizontal tumbling motion, and tilts the actuator so that its axis is oriented with a horizontal component and continued actuation generates both vertical and horizontal force.

  14. Optical detection of radio waves through a nanomechanical transducer

    Science.gov (United States)

    Bagci, T.; Simonsen, A.; Schmid, S.; Villanueva, L. G.; Zeuthen, E.; Appel, J.; Taylor, J. M.; Sørensen, A.; Usami, K.; Schliesser, A.; Polzik, E. S.

    2014-03-01

    Low-loss transmission and sensitive recovery of weak radio-frequency and microwave signals is a ubiquitous challenge, crucial in radio astronomy, medical imaging, navigation, and classical and quantum communication. Efficient up-conversion of radio-frequency signals to an optical carrier would enable their transmission through optical fibres instead of through copper wires, drastically reducing losses, and would give access to the set of established quantum optical techniques that are routinely used in quantum-limited signal detection. Research in cavity optomechanics has shown that nanomechanical oscillators can couple strongly to either microwave or optical fields. Here we demonstrate a room-temperature optoelectromechanical transducer with both these functionalities, following a recent proposal using a high-quality nanomembrane. A voltage bias of less than 10 V is sufficient to induce strong coupling between the voltage fluctuations in a radio-frequency resonance circuit and the membrane's displacement, which is simultaneously coupled to light reflected off its surface. The radio-frequency signals are detected as an optical phase shift with quantum-limited sensitivity. The corresponding half-wave voltage is in the microvolt range, orders of magnitude less than that of standard optical modulators. The noise of the transducer--beyond the measured Johnson noise of the resonant circuit--consists of the quantum noise of light and thermal fluctuations of the membrane, dominating the noise floor in potential applications in radio astronomy and nuclear magnetic imaging. Each of these contributions is inferred to be when balanced by choosing an electromechanical cooperativity of with an optical power of 1 mW. The noise temperature of the membrane is divided by the cooperativity. For the highest observed cooperativity of , this leads to a projected noise temperature of 40 mK and a sensitivity limit of . Our approach to all-optical, ultralow-noise detection of classical

  15. High-Temperature Surface-Acoustic-Wave Transducer

    Science.gov (United States)

    Zhao, Xiaoliang; Tittmann, Bernhard R.

    2010-01-01

    Aircraft-engine rotating equipment usually operates at high temperature and stress. Non-invasive inspection of microcracks in those components poses a challenge for the non-destructive evaluation community. A low-profile ultrasonic guided wave sensor can detect cracks in situ. The key feature of the sensor is that it should withstand high temperatures and excite strong surface wave energy to inspect surface/subsurface cracks. As far as the innovators know at the time of this reporting, there is no existing sensor that is mounted to the rotor disks for crack inspection; the most often used technology includes fluorescent penetrant inspection or eddy-current probes for disassembled part inspection. An efficient, high-temperature, low-profile surface acoustic wave transducer design has been identified and tested for nondestructive evaluation of structures or materials. The development is a Sol-Gel bismuth titanate-based surface-acoustic-wave (SAW) sensor that can generate efficient surface acoustic waves for crack inspection. The produced sensor is very thin (submillimeter), and can generate surface waves up to 540 C. Finite element analysis of the SAW transducer design was performed to predict the sensor behavior, and experimental studies confirmed the results. One major uniqueness of the Sol-Gel bismuth titanate SAW sensor is that it is easy to implement to structures of various shapes. With a spray coating process, the sensor can be applied to surfaces of large curvatures. Second, the sensor is very thin (as a coating) and has very minimal effect on airflow or rotating equipment imbalance. Third, it can withstand temperatures up to 530 C, which is very useful for engine applications where high temperature is an issue.

  16. Reliability and validity assessment of a linear position transducer.

    Science.gov (United States)

    Garnacho-Castaño, Manuel V; López-Lastra, Silvia; Maté-Muñoz, José L

    2015-03-01

    The objectives of the study were to determine the validity and reliability of peak velocity (PV), average velocity (AV), peak power (PP) and average power (AP) measurements were made using a linear position transducer. Validity was assessed by comparing measurements simultaneously obtained using the Tendo Weightlifting Analyzer Systemi and T-Force Dynamic Measurement Systemr (Ergotech, Murcia, Spain) during two resistance exercises, bench press (BP) and full back squat (BS), performed by 71 trained male subjects. For the reliability study, a further 32 men completed both lifts using the Tendo Weightlifting Analyzer Systemz in two identical testing sessions one week apart (session 1 vs. session 2). Intraclass correlation coefficients (ICCs) indicating the validity of the Tendo Weightlifting Analyzer Systemi were high, with values ranging from 0.853 to 0.989. Systematic biases and random errors were low to moderate for almost all variables, being higher in the case of PP (bias ±157.56 W; error ±131.84 W). Proportional biases were identified for almost all variables. Test-retest reliability was strong with ICCs ranging from 0.922 to 0.988. Reliability results also showed minimal systematic biases and random errors, which were only significant for PP (bias -19.19 W; error ±67.57 W). Only PV recorded in the BS showed no significant proportional bias. The Tendo Weightlifting Analyzer Systemi emerged as a reliable system for measuring movement velocity and estimating power in resistance exercises. The low biases and random errors observed here (mainly AV, AP) make this device a useful tool for monitoring resistance training. Key pointsThis study determined the validity and reliability of peak velocity, average velocity, peak power and average power measurements made using a linear position transducerThe Tendo Weight-lifting Analyzer Systemi emerged as a reliable system for measuring movement velocity and power. PMID:25729300

  17. Focusing Modeling of OPFC Linear Array Transducer by Using Distributed Point Source Method

    Directory of Open Access Journals (Sweden)

    Ziping Wang

    2014-01-01

    Full Text Available The improvement of ultrasonic phased array detection technology is a major concern of engineering community. Orthotropic piezoelectric fiber composite (OPFC can be constructed to multielement linear array which may be applied conveniently to actuators and sensors. The phased array transducers can generate special directional strong actuator power and high sensitivity for its orthotropic performance. Focusing beam of the linear phased array transducer is obtained simply only by adjusting a parabolic time delay. In this work, the distributed point source method (DPSM is used to model the ultrasonic field. DPSM is a newly developed mesh-free numerical technique that has been developed for solving a variety of engineering problems. This work gives the basic theory of this method and solves the problems from the application of new OPFC phased array transducer. Compared with traditional transducer, the interaction effect of two OPFC linear phased array transducers is also modeled in the same medium, which shows that the pressure beam produced by the new transducer is narrower or more collimated than that produced by the conventional transducer at different angles. DPSM can be used to analyze and optimally design the OPFC linear phased array transducer.

  18. Static and dynamic characteristics of angular velocity and acceleration transducers based on optical tunneling effect

    Science.gov (United States)

    Busurin, V. I.; Korobkov, V. V.; Htoo Lwin, Naing; Tuan, Phan Anh

    2016-08-01

    Theoretical and experimental analysis of quasi-linear conversion function of angular velocity and acceleration microoptoelectromechnical (MOEM) transducers based on optical tunneling effect (OTE) are conducted. Equivalent oscillating circuit is developed and dynamic characteristics of angular velocity and acceleration MOEM-transducers are investigated.

  19. Acoustical cross-talk in row–column addressed 2-D transducer arrays for ultrasound imaging

    DEFF Research Database (Denmark)

    Christiansen, Thomas Lehrmann; Jensen, Jørgen Arendt; Thomsen, Erik Vilain

    2015-01-01

    The acoustical cross-talk in row–column addressed 2-D transducer arrays for volumetric ultrasound imaging is investigated. Experimental results from a 2.7 MHz, λ/2-pitch capacitive micromachined ultrasonic transducer (CMUT) array with 62 rows and 62 columns are presented and analyzed in the...

  20. Design, development and metrological characterization of a low capacity precision industrial force transducer.

    Science.gov (United States)

    Kumar, Harish; Sharma, Chitra; Kumar, Anil; Arora, P K; Kumar, S

    2015-09-01

    The paper discusses the development of the ring shaped force transducers for measurement of force in lower capacity to meet the industrial requirements with the increasing technological developments. A 50 N ring shaped force transducer for tension mode has been developed by studying the analytical and computational methods. The force transducer developed has been metrologically studied according to the calibration procedure based on the standard ISO 376 and uncertainty of measurement of the force transducer is found to be±0.10% (k=2), while taking into account the relative uncertainty contribution due to necessary factors like repeatability, reproducibility, zero offset, interpolation, resolution and reversibility. The force transducer developed may further be studied for improvement of metrological performance and may suitably be developed for other lower capacities like 10 N, 20 N etc. The force transducer developed offers very economical alternative of complex shaped force transducers with simple design and manufacturing features. The force transducer developed may be proved very helpful in providing traceability to the user industries and calibration laboratories in the lower range of force measurement and serve as force transfer standard.

  1. Echo signal from rough planar interfaces influence of roughness, angle, range and transducer type

    DEFF Research Database (Denmark)

    Wilhjelm, Jens E.; Pedersen, P.C.; Jacobsen, S.M.;

    1998-01-01

    The received electrical signal from a pulse-echo system insonifying a planar acoustical interface was measured for varying degrees of rms roughness (0-0.16 mm), angle of incidence (typically +/-7°) and range to the transducer. A planar and a focused 5 MHz transducer was used. When insonifying...

  2. First measurements on a novel type of optical micro-machined ultrasound transducer (OMUT)

    NARCIS (Netherlands)

    Leinders, S.M.; Dongen, K.W.A. van; Jong, N. de; Verweij, M.D.; Westerveld, W.J.; Urbach, H.P.; Neer, P.L.M.J. van; Pozo Torres, J.M.

    2014-01-01

    Several types of ultrasound sensors have been developed and are used in the field of medical imaging. Conventional transducers are made of piezo-electric material and show good practical performance. However, when the piezo-electric elements need to be small (below 100 μm × 100 μm), these transducer

  3. Effects of size and arrangement of virtual transducer on photoacoustic tomography

    Institute of Scientific and Technical Information of China (English)

    Wang Shao-Hua; Tao Chao; Liu Xiao-Jun

    2013-01-01

    In this paper,we investigate the effects of the relative size and arrangement of a virtual transducer on the image quality in limited-view photoacoustic tomography.A virtual transducer refers to the acoustic scatterers used to reflect photoacoustic waves and improve the images reconstructed from incomplete PA signal.Size and spatial arrangement determine the performance of the virtual transducer.In this study,the scatterers utilized as virtual transducers are arranged in different manners,such as on a straight line or on an arc line.We find that virtual transducers with a big distributing angle can provide more significant image improvement than with a small distributing angle,which is similar to the true transducers.We also change the size of virtual transducer and study its influence on image quality.It is found that the bigger scatterers provide better images than the smaller ones.Especially,when the size of scatterers is reduced to the wavelength of photoacoustic wave,the image quality observably decreases,owing to the strong diffraction effect.Thus,it is suggested that the size of the acoustical scatterers should be much larger than the photoacoustic wavelength.The simulations are conducted,and the results could be helpful for the application and further study of virtual transducer theory in limited-view photoacoustic tomography.

  4. Study on the sandwich piezoelectric ceramic ultrasonic transducer in thickness vibration

    International Nuclear Information System (INIS)

    A sandwich piezoelectric ceramic ultrasonic transducer in thickness vibration is studied. The transducer consists of front and back metal masses, and coaxially segmented, thickness polarized piezoelectric ceramic thin rings. For this kind of sandwich piezoelectric transducers in thickness vibration, it is required that the lateral dimension of the transducer is sufficiently large compared with its longitudinal dimension so that no lateral displacements in the transducer can occur (laterally clamped). In this paper, the thickness vibration of the piezoelectric ceramic stack consisting of a number of identical piezoelectric ceramic thin rings is analysed and its electro-mechanical equivalent circuit is obtained. The resonance frequency equation for the sandwich piezoelectric ceramic ultrasonic transducer in thickness vibration is derived. Based on the frequency equation, two sandwich piezoelectric ceramic ultrasonic transducers are designed and manufactured, and their resonance frequencies are measured. It is shown that the measured resonance frequencies are in good agreement with the theoretical results. This kind of sandwich piezoelectric ultrasonic transducer is expected to be used in megasonic ultrasonic cleaning and sonochemistry where high power and high frequency ultrasound is needed

  5. Transducer combination for high-quality ultrasound tomography based on speed of sound imaging

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Young Hun; Park, Kwan Kyu [Dept. of Mechanical Engineering, Hanyang University, Seoul (Korea, Republic of)

    2016-02-15

    The type of ultrasound transducer used influences the quality of a reconstructed ultrasound image. This study analyzed the effect of transducer type on ultrasound computed tomography (UCT) image quality. The UCT was modeled in an ultrasound simulator by using a 5 cm anatomy model and a ring-shape 5 MHz 128 transducer array, which considered attenuation, refraction, and reflection. Speed-of-sound images were reconstructed by the Radon transform as the UCT image modality. Acoustic impedance images were also reconstructed by the delayand-sum (DAS) method, which considered the speed of sound information. To determine the optimal combination of transducers in observation, point-source, flat, and focused transducers were tested in combination as trasmitters and receivers; UCT images were constructed from each combination. The combination of point-source/flat transducer as transmitting and receiving devices presented the best reconstructed image quality. In UCT implementation, the combination of a flat transducer for transmitting and a point transducer for receiving permitted acceptable image quality.

  6. Air-coupled piezoelectric transducers with active polypropylene foam matching layers.

    Science.gov (United States)

    Gómez Alvarez-Arenas, Tomás E

    2013-05-10

    This work presents the design, construction and characterization of air-coupled piezoelectric transducers using 1-3 connectivity piezocomposite disks with a stack of matching layers being the outer one an active quarter wavelength layer made of polypropylene foam ferroelectret film. This kind of material has shown a stable piezoelectric response together with a very low acoustic impedance (polypropylene foam ferroelectret film (0.35 MHz), then, the rest of the transducer components (piezocomposite disk and passive intermediate matching layers) are all tuned to this frequency. The transducer has been tested in several working modes including pulse-echo and pitch-catch as well as wide and narrow band excitation. The performance of the proposed novel transducer is compared with that of a conventional air-coupled transducers operating in a similar frequency range.

  7. Directivity calculation with experimental verification for a conformal array of underwater acoustic transducers

    Institute of Scientific and Technical Information of China (English)

    HE Zhengyao; MA Yuanliang

    2008-01-01

    The radiation directivity of a complicated conformal array of underwater acoustic transducers is presented based on the boundary 'element method. It includes the element directivity of each transducer, the natural beam pattern and the controlled beam pattern of the transducer array. At first, the boundary element model of the conformal array is built up, and then the boundary condition is exerted on the model according to the design and environment in which the transducer array is used, and the radiation directivity of the conformal array is calculated. An experiment has been done to measure the directivity in an anechoic water tank.The calculated and the experimental results are compared and analyzed. They are consistentto each other. It is shown that the boundary element method together with the detailed calculations is successful to simulate and predict the radiation directivity of an underwater acoustic transducer array.

  8. Metrological analysis of a virtual flowmeter-based transducer for cryogenic helium

    Science.gov (United States)

    Arpaia, P.; Girone, M.; Liccardo, A.; Pezzetti, M.; Piccinelli, F.

    2015-12-01

    The metrological performance of a virtual flowmeter-based transducer for monitoring helium under cryogenic conditions is assessed. At this aim, an uncertainty model of the transducer, mainly based on a valve model, exploiting finite-element approach, and a virtual flowmeter model, based on the Sereg-Schlumberger method, are presented. The models are validated experimentally on a case study for helium monitoring in cryogenic systems at the European Organization for Nuclear Research (CERN). The impact of uncertainty sources on the transducer metrological performance is assessed by a sensitivity analysis, based on statistical experiment design and analysis of variance. In this way, the uncertainty sources most influencing metrological performance of the transducer are singled out over the input range as a whole, at varying operating and setting conditions. This analysis turns out to be important for CERN cryogenics operation because the metrological design of the transducer is validated, and its components and working conditions with critical specifications for future improvements are identified.

  9. Metrological analysis of a virtual flowmeter-based transducer for cryogenic helium

    Energy Technology Data Exchange (ETDEWEB)

    Arpaia, P., E-mail: pasquale.arpaia@unina.it [Department of Electrical Engineering and Information Technology, University of Napoli Federico II, Naples (Italy); Technology Department, European Organization for Nuclear Research (CERN), Geneva (Switzerland); Girone, M., E-mail: mario.girone@cern.ch [Technology Department, European Organization for Nuclear Research (CERN), Geneva (Switzerland); Department of Engineering, University of Sannio, Benevento (Italy); Liccardo, A., E-mail: annalisa.liccardo@unina.it [Department of Electrical Engineering and Information Technology, University of Napoli Federico II, Naples (Italy); Pezzetti, M., E-mail: marco.pezzetti@cern.ch [Technology Department, European Organization for Nuclear Research (CERN), Geneva (Switzerland); Piccinelli, F., E-mail: fabio.piccinelli@cern.ch [Department of Mechanical Engineering, University of Brescia, Brescia (Italy)

    2015-12-15

    The metrological performance of a virtual flowmeter-based transducer for monitoring helium under cryogenic conditions is assessed. At this aim, an uncertainty model of the transducer, mainly based on a valve model, exploiting finite-element approach, and a virtual flowmeter model, based on the Sereg-Schlumberger method, are presented. The models are validated experimentally on a case study for helium monitoring in cryogenic systems at the European Organization for Nuclear Research (CERN). The impact of uncertainty sources on the transducer metrological performance is assessed by a sensitivity analysis, based on statistical experiment design and analysis of variance. In this way, the uncertainty sources most influencing metrological performance of the transducer are singled out over the input range as a whole, at varying operating and setting conditions. This analysis turns out to be important for CERN cryogenics operation because the metrological design of the transducer is validated, and its components and working conditions with critical specifications for future improvements are identified.

  10. Loss effects on adhesively-bonded multilayer ultrasonic transducers by self-heating.

    Science.gov (United States)

    Wu, Zhengbin; Cochran, Sandy

    2010-04-01

    Multilayer ultrasonic transducers are widely being used for high power applications. In these applications, typical Langevin/Tonpilz structures without any adhesive bondings however have the disadvantage of limited bandwidth. Therefore adhesively-bonded structures are still a potential solution for this issue. In this paper, two-layer piezoelectric ceramic ultrasonic transducers with two different adhesive bondlines were investigated comparing to a single-layer transducer in terms of loss effects during operation with excitation signals sufficient to cause self-heating. The theoretical functions fitted to the measured time-temperature dependency data are compared with experimental results of different piezoelectric transducers. Theoretical analysis of loss characteristics at various surface displacements and the relationship with increasing temperature are reported. The effects of self-heating on the practical performance of multilayer ultrasonic transducers with adhesive bondlines are discussed. PMID:19942247

  11. Metrological analysis of a virtual flowmeter-based transducer for cryogenic helium

    International Nuclear Information System (INIS)

    The metrological performance of a virtual flowmeter-based transducer for monitoring helium under cryogenic conditions is assessed. At this aim, an uncertainty model of the transducer, mainly based on a valve model, exploiting finite-element approach, and a virtual flowmeter model, based on the Sereg-Schlumberger method, are presented. The models are validated experimentally on a case study for helium monitoring in cryogenic systems at the European Organization for Nuclear Research (CERN). The impact of uncertainty sources on the transducer metrological performance is assessed by a sensitivity analysis, based on statistical experiment design and analysis of variance. In this way, the uncertainty sources most influencing metrological performance of the transducer are singled out over the input range as a whole, at varying operating and setting conditions. This analysis turns out to be important for CERN cryogenics operation because the metrological design of the transducer is validated, and its components and working conditions with critical specifications for future improvements are identified

  12. Application of PMN-32PT Piezoelectric Crystals for Novel Air-coupled Ultrasonic Transducers

    Science.gov (United States)

    Kazys, Rymantas Jonas; Sliteris, Reimondas; Sestoke, Justina

    Due to very high piezoelectric properties of PMN-PT crystals they may significantly improve performance of air-coupled ultrasonic transducers. For these purpose vibrations of PMN-PT rectangular plates and strips were investigated. An air-coupled ultrasonic transducer and array consisting of 8 single piezoelectric strips were designed. Operation of the transducer was simulated by the finite element method using ANSYS Mechanical APDL Product Launcher software. Spatial distributions of displacements inside piezoelectric elements and matching strip were obtained. Experimental investigations were carried out by the laser Doppler vibrometer Polytec OFV-5000 and the Bruel&Kjaer microphone 4138 with the measurement amplifier NEXUS WH 3219. It was found that performance of the ultrasonic transducer with PMN-32PT crystals was a few times better than of a PZT based ultrasonic transducer.

  13. Note: Decoupling design for high frequency piezoelectric ultrasonic transducers with their clamping connections

    Energy Technology Data Exchange (ETDEWEB)

    Wang, F. J., E-mail: wangfujun@tju.edu.cn; Liang, C. M.; Tian, Y. L.; Zhao, X. Y.; Zhang, D. W. [Tianjin Key Laboratory of Equipment Design and Manufacturing Technology, School of Mechanical Engineering, Tianjin University, Tianjin 300072 (China); Zhang, H. J. [Tianjin Key Laboratory of Modern Mechatronics Equipment Technology, School of Mechanical Engineering, Tianjin Polytechnic University, Tianjin 300387 (China)

    2015-12-15

    This work presents the flexure-mechanism based decoupling design between high frequency piezoelectric ultrasonic transducers and their clamping connections to improve ultrasonic energy transmission efficiency. The ring, prismatic beam, and circular notched hinge based flanges were presented, and the crucial geometric dimensions of the transducers with the flexure decoupling flanges were determined. Finite element analysis (FEA) was carried out to investigate the dynamic characteristics of the transducers. Finally, experiments were conducted to examine and verify the effects of the proposed decoupling flanges. FEA and experimental results show that smaller frequency deviations and larger tip displacement amplitudes have been achieved by using the transducers with the flexure flanges compared with the transducer with a rigid ring-type flange, and thus the ultrasonic transmission efficiency can be improved through the flexure flanges.

  14. Metrological analysis of a virtual flowmeter-based transducer for cryogenic helium.

    Science.gov (United States)

    Arpaia, P; Girone, M; Liccardo, A; Pezzetti, M; Piccinelli, F

    2015-12-01

    The metrological performance of a virtual flowmeter-based transducer for monitoring helium under cryogenic conditions is assessed. At this aim, an uncertainty model of the transducer, mainly based on a valve model, exploiting finite-element approach, and a virtual flowmeter model, based on the Sereg-Schlumberger method, are presented. The models are validated experimentally on a case study for helium monitoring in cryogenic systems at the European Organization for Nuclear Research (CERN). The impact of uncertainty sources on the transducer metrological performance is assessed by a sensitivity analysis, based on statistical experiment design and analysis of variance. In this way, the uncertainty sources most influencing metrological performance of the transducer are singled out over the input range as a whole, at varying operating and setting conditions. This analysis turns out to be important for CERN cryogenics operation because the metrological design of the transducer is validated, and its components and working conditions with critical specifications for future improvements are identified.

  15. Modeling transducer impulse responses for predicting calibrated pressure pulses with the ultrasound simulation program Field II

    DEFF Research Database (Denmark)

    Bæk, David; Jensen, Jørgen Arendt; Willatzen, Morten

    2010-01-01

    FIELD II is a simulation software capable of predicting the field pressure in front of transducers having any complicated geometry. A calibrated prediction with this program is, however, dependent on an exact voltage-to-surface acceleration impulse response of the transducer. Such impulse response...... is not calculated by FIELD II. This work investigates the usability of combining a one-dimensional multilayer transducer modeling principle with the FIELD II software. Multilayer here refers to a transducer composed of several material layers. Measurements of pressure and current from Pz27 piezoceramic disks...... as well as pressure and intensity measurements in front of a 128 element commercial convex medical transducer are compared to the simulations. Results show that the models can predict the pressure from the piezoceramic disks with a root mean square (rms) error of 11.2% to 36.2% with a 2 dB amplitude...

  16. Effects of boundary conditions on vibrating mode of acoustic logging dipole transducer

    Institute of Scientific and Technical Information of China (English)

    2008-01-01

    Acoustic transducer is an important part of acoustic well logging tool. In this paper,ANSYS software package is used to design acoustic dipole transducer and simulate vibrating mode of the dipole transducer in different mechanical boundary conditions. The results show that boundary conditions influence the number of vibrating mode in the same frequency band and the frequency value of the same vibrating mode. Several acoustic dipole transducers are designed according to the results of numerical simulation and laboratory measurements. The basic frequency of vibrating mode of experi-ment has good agreement with that of simulation. The numerical simulation plays a good guidance role in designing,producing and correctly installing the acoustic dipole transducer.

  17. A new three-dimensional electromechanical impedance model for an embedded dual-PZT transducer

    Science.gov (United States)

    Wang, Dansheng; Li, Zhi; Zhu, Hongping

    2016-07-01

    In the past twenty years, the electromechanical (EM) impedance technique has been investigated extensively in the mechanical, aviation and civil engineering fields. Many different EM impedance models have been proposed to characterize the interaction between the surface-bonded PZT transducer and the host structure. This paper formulates a new three-dimensional EM impedance model characterizing the interaction between an embedded circle dual-PZT transducer and the host structure based on the effective impedance concept. The proposed model is validated by experimental results from a group of smart cement cubes, in which three circle dual-PZT transducers are embedded respectively. In addition, a new EM impedance measuring method for the dual-PZT transducer is also introduced. In the measuring method, only a common signal generator and an oscilloscope are needed, by which the exciting and receiving voltage signals are obtained respectively. Combined with fast Fourier transform the EM impedance signatures of the dual-PZT transducers are obtained.

  18. Partitioning mechanisms of masking: contrast transducer versus divisive inhibition

    Science.gov (United States)

    Barghout-Stein, Lauren; Tyler, Christopher W.; Klein, Stanley A.

    1997-06-01

    noise that intrudes on the detecting mechanism from neighboring mechanisms. A detailed review of this debate is provided by the paper by Klein et al., 3016-02 in this Proceedings. Threshold elevation functions that show the relationship between mask spatial frequency and masking magnitude cannot illuminate this debate, as we demonstrated at ARVO (1994). For that study, we generated threshold elevation functions (the ratio of unmasked versus masked target threshold) for multi-channel systems using computational models that invoked either divisive inhibition, a set of transducer nonlinearities or multiplicative noise. Threshold elevation functions were indistinguishable when each masking process was assumed to have similar strength. These results led us to design the experiment presented here, which attempts to compare the effects of two of these masking processes, lateral divisive inhibition and nonlinear transducer compression.

  19. Growth stimulation of Bacillus cereus and Pseudomonas putida using nanostructured ZnO thin film as transducer element

    Energy Technology Data Exchange (ETDEWEB)

    Loukanov, Alexandre, E-mail: loukanov@mail.saitama-u.ac.jp [Saitama University, Department of Chemistry, Faculty of Science (Japan); Filipov, Chavdar [University of Forestry, Department of Infectious pathology, hygiene, technology and control of food stuffs of animal origin, Faculty of Veterinary Medicine (Bulgaria); Valcheva, Violeta [Bulgarian Academy of Science, Department of Infectious Diseases, Institute of microbiology (Bulgaria); Lecheva, Marta [University of Mining and Geology “St. Ivan Rilski”, Laboratory of Engineering NanoBiotechnology, Department of Engineering Geoecology (Bulgaria); Emin, Saim [University of Nova Gorica, Materials Research Laboratory (Slovenia)

    2015-04-15

    The semiconductor zinc oxide nanomaterial (ZnO or ZnO:H) is widely used in advanced biosensor technology for the design of highly-sensitive detector elements for various applications. In the attempt to evaluate its effect on common microorganisms, two types of nanostructured transducer films have been used (average diameter 600–1000 nm). They have been prepared by using both wet sol–gel method and magnetron sputtering. Their polycrystalline structure and specific surface features have been analyzed by X-ray diffraction (XRD), scanning electron microscope, and atomic force microscope. The assessment of growth stimulation of bacteria was determined using epifluorescent microscope by cell staining with Live/Dead BacLight kit. In our experiments, the growth stimulation of Gram-positive and Gram-negative bacteria on nanostructured ZnO film is demonstrated by Bacillus cereus and Pseudomonas putida. These two bacterial species have been selected, because they are well known and studied in biosensor technologies, with structural difference of their cell walls. These pathogens are easy for with common source in the liquid food or some commercial products. Our data has revealed that the method of transducer film preparation influences strongly bacterial inhibition and division. These results present the transforming signal precisely, when ZnO is used in biosensor applications.

  20. Growth stimulation of Bacillus cereus and Pseudomonas putida using nanostructured ZnO thin film as transducer element

    International Nuclear Information System (INIS)

    The semiconductor zinc oxide nanomaterial (ZnO or ZnO:H) is widely used in advanced biosensor technology for the design of highly-sensitive detector elements for various applications. In the attempt to evaluate its effect on common microorganisms, two types of nanostructured transducer films have been used (average diameter 600–1000 nm). They have been prepared by using both wet sol–gel method and magnetron sputtering. Their polycrystalline structure and specific surface features have been analyzed by X-ray diffraction (XRD), scanning electron microscope, and atomic force microscope. The assessment of growth stimulation of bacteria was determined using epifluorescent microscope by cell staining with Live/Dead BacLight kit. In our experiments, the growth stimulation of Gram-positive and Gram-negative bacteria on nanostructured ZnO film is demonstrated by Bacillus cereus and Pseudomonas putida. These two bacterial species have been selected, because they are well known and studied in biosensor technologies, with structural difference of their cell walls. These pathogens are easy for with common source in the liquid food or some commercial products. Our data has revealed that the method of transducer film preparation influences strongly bacterial inhibition and division. These results present the transforming signal precisely, when ZnO is used in biosensor applications

  1. Three-dimensional architecture of hair-cell linkages as revealedby electron-microscopic tomography

    Energy Technology Data Exchange (ETDEWEB)

    Auer, Manfred; Koster, Bram; Ziese, Ulrike; Bajaj, Chandrajit; Volkmann, Niels; Wang, Da Neng; Hudspeth, A. James

    2006-07-28

    The senses of hearing and balance rest upon mechanoelectrical transduction by the hair bundles of hair cells in the inner ear. Located at the apical cellular surface, each hair bundle comprises several tens of stereocilia and a single kinocilium that are interconnected by extracellular proteinaceous links. Using electron-microscopic tomography of bullfrog saccular sensory epithelia, we examined the three-dimensional structures of ankle or basal links, kinociliary links, and tip links. We observed clear differences in the dimensions and appearances of the three links. We found two distinct populations of tip links suggestive of the involvement of two proteins or splice variants. We noted auxiliary links connecting the upper portions of tip links to the taller stereocilia. Tip links and auxiliary links show a tendency to adopt a globular conformation when disconnected from the membrane surface.

  2. Toll-like receptor 4, a novel signal transducer for lipopolysaccharide

    Institute of Scientific and Technical Information of China (English)

    杨清武; 朱佩芳; 王正国; 蒋建新

    2002-01-01

    @@Lipopolysaccharide (LPS), or endotoxin, is the major component of the outer surface of gram-negative bacteria. LPS is a potent activator of the cells of the immune and inflammation systems, including macrophages, monocytes and endothelial cells, and contributes to systemic changes seen in septic shock.1,2 It has long been believed that LPS is responsible for several fatal consequences of gram-negative infection. Cell activation by LPS constitutes the first step in the cascade of events believed to lead to the manifestation of gram-negative sepsis, which results in approximately 20 000 annual deaths in the United States3 and 30% mortality rate of known cases in China.Therefore, the action mechanism of LPS is one of the most important problems in the research field of immunity, inflammation and surgery. Researchers have investigated the mechanism of cell activity and injury of LPS for a long time. In 1990, CD14,the glycosyl-phosphatidylinositol (GPI)-linked plasma membrane protein, was identified as a proximal LPS receptor on the cell surface of macrophages, and it was suggested that CD14 and LBP (lipopolysaccharide binding protein) played an important role in the effect mechanism of LPS. CD14 seemed to receive LPS via transfer from the plasma protein LBP. Then, two action patterns were recognized. CD14 positive cells, such as macrophages and leukocytes, were activated after LPS combined with LBP and interacted with CD14. But, CD14 negative cells (for example, endothelial cells), were activated through other receptors that we did not know of in the cell surface after LPS, LBP and soluble CD14 (sCD14) combined with the compounds. However, there are some questions to be answered. Firstly, because CD14 lacks cytoplasmic doman, it is unlikely to act as the transducer. Secondly, the action pattern of LPS through CD14 and LBP may be in dose-dependent mode, but, in conditions of high dosage and long exposure to LPS action, CD14 and LBP do not play an important role in

  3. Phased annular array transducers for omnidirectional guided wave mode control in isotropic plate like structures

    International Nuclear Information System (INIS)

    Ultrasonic guided waves are fast emerging as a reliable tool for continuous structural health monitoring. Their multi-modal nature along with their long range propagation characteristics offer several possibilities for interrogating structures. Transducers commonly used to generate guided waves in structures excite multiple modes at any frequency; their complex scattering and reflection from defects and boundaries often complicates the extraction of useful information. Often it is desirable to control the guided wave modes propagating in a structure to take advantage of their unique properties for different applications. Earlier attempts at guided wave mode control involved developing fixed wavelength linear and annular array transducers. Their only disadvantage is that the transducer is limited to a particular wavelength and a change in wavelength necessitates a change in the transducer. In this paper, we propose the development of an annular array transducer that can generate mode controlled omnidirectional guided waves by independently controlling the amplitude and phase of the array elements. A simplified actuator model that approximates the transducer loading on the structure to a constant pressure load under the array elements is assumed and an optimization problem is set up to compute the excitation voltage and phase of the elements. A five element annular array transducer is designed utilizing 1–3 type piezocomposite materials. The theoretical computations are experimentally verified on an aluminum plate like structure by exciting A0 and S0 guided wave modes. (paper)

  4. Design and Development of a Pressure Transducer for High Hydrostatic Pressure Measurements up to 200 MPa

    Science.gov (United States)

    Kumar, Anuj; Yadav, Sanjay; Agarwal, Ravinder

    2016-06-01

    A number of pressure transducers, based on strain gauge, capacitance/inductance type, frequency resonators, are commercially available and are being used for sensing and producing an electrical output proportional to applied pressure. These sensors have their own advantages and limitations due to operational ease, measurement uncertainty and the costs. Strain gauge type transducers are now well established devices for accurate and precise measurement of pressure within measurement uncertainty up to 0.1 % of full scale. In the present research work, an indigenous strain gauge pressure transducer has been designed, developed, tested and calibrated for pressure measurement up to 200 MPa. The measurement uncertainty estimated using the pressure transducer was found better than 0.1 % of full scale. This transducer was developed using four foil type strain gauges, bonded, two in axial direction while other two in radial direction, to the controlled stress zones of a tubular maraging steel active cylinder working also as diaphragm. The strain gages were then connected to a Wheatstone bridge arrangement to measure stress generated strains. The pressure was applied through matching connector designed in the same tubular transducer active element. The threaded unique design in a single piece through collar, ferule and tubing arrangement provides leak proof pressure connections with external devices without using additional seals. The calibration and performance checking of the pressure transducer was carried out using dead weight type national pressure standard using the internationally accepted calibration procedure.

  5. High Performance Relaxor-Based Ferroelectric Single Crystals for Ultrasonic Transducer Applications

    Directory of Open Access Journals (Sweden)

    Yan Chen

    2014-07-01

    Full Text Available Relaxor-based ferroelectric single crystals Pb(Mg1/3Nb2/3O3-PbTiO3 (PMN-PT have drawn much attention in the ferroelectric field because of their excellent piezoelectric properties and high electromechanical coupling coefficients (d33~2000 pC/N, kt~60% near the morphotropic phase boundary (MPB. Ternary Pb(In1/2Nb1/2O3-Pb(Mg1/3Nb2/3O3-PbTiO3 (PIN-PMN-PT single crystals also possess outstanding performance comparable with PMN-PT single crystals, but have higher phase transition temperatures (rhombohedral to tetragonal Trt, and tetragonal to cubic Tc and larger coercive field Ec. Therefore, these relaxor-based single crystals have been extensively employed for ultrasonic transducer applications. In this paper, an overview of our work and perspectives on using PMN-PT and PIN-PMN-PT single crystals for ultrasonic transducer applications is presented. Various types of single-element ultrasonic transducers, including endoscopic transducers, intravascular transducers, high-frequency and high-temperature transducers fabricated using the PMN-PT and PIN-PMN-PT crystals and their 2-2 and 1-3 composites are reported. Besides, the fabrication and characterization of the array transducers, such as phased array, cylindrical shaped linear array, high-temperature linear array, radial endoscopic array, and annular array, are also addressed.

  6. High performance relaxor-based ferroelectric single crystals for ultrasonic transducer applications.

    Science.gov (United States)

    Chen, Yan; Lam, Kwok-Ho; Zhou, Dan; Yue, Qingwen; Yu, Yanxiong; Wu, Jinchuan; Qiu, Weibao; Sun, Lei; Zhang, Chao; Luo, Haosu; Chan, Helen L W; Dai, Jiyan

    2014-01-01

    Relaxor-based ferroelectric single crystals Pb(Mg1/3Nb2/3)O3-PbTiO3 (PMN-PT) have drawn much attention in the ferroelectric field because of their excellent piezoelectric properties and high electromechanical coupling coefficients (d33~2000 pC/N, kt~60%) near the morphotropic phase boundary (MPB). Ternary Pb(In1/2Nb1/2)O3-Pb(Mg1/3Nb2/3)O3-PbTiO3 (PIN-PMN-PT) single crystals also possess outstanding performance comparable with PMN-PT single crystals, but have higher phase transition temperatures (rhombohedral to tetragonal Trt, and tetragonal to cubic Tc) and larger coercive field Ec. Therefore, these relaxor-based single crystals have been extensively employed for ultrasonic transducer applications. In this paper, an overview of our work and perspectives on using PMN-PT and PIN-PMN-PT single crystals for ultrasonic transducer applications is presented. Various types of single-element ultrasonic transducers, including endoscopic transducers, intravascular transducers, high-frequency and high-temperature transducers fabricated using the PMN-PT and PIN-PMN-PT crystals and their 2-2 and 1-3 composites are reported. Besides, the fabrication and characterization of the array transducers, such as phased array, cylindrical shaped linear array, high-temperature linear array, radial endoscopic array, and annular array, are also addressed. PMID:25076222

  7. Influence of resonant transducer variations on long range guided wave monitoring of rail track

    Science.gov (United States)

    Loveday, Philip W.; Long, Craig S.

    2016-02-01

    The ability of certain guided wave modes to propagate long distances in continuously welded rail track is exploited in permanently installed monitoring systems. Previous work demonstrated that reflections from thermite welds could be measured at distances of the order of 1 km from a transducer array. The availability of numerous thermite welds is useful during the development of a monitoring system as real defects are not available. Measurements of reflections from welds were performed over an eleven month period with two permanently installed transducers. Phased array processing was performed and the true location of a weld is indicated by a strong reflection but there is generally also a smaller, spurious replica reflection, at the same distance but in the incorrect direction. In addition, the relative reflection from different welds appears to change over time. The influence of differences between the two resonant transducers was investigated using a model. It was found that estimating the attenuation in either direction and scaling the reflections in either direction decreased the variability in the reflection measurements. Transducer interaction effects, where the transducer closer to the weld records a greater reflection than the second transducer were observed and can be used to determine the direction of a weld. This feature was used to demonstrate a simple alternative to phased array processing that can be used with resonant transducers.

  8. Research on the Low Frequency Broadband Piezoelectric-Magnetostrictive Hybrid Transducer

    Directory of Open Access Journals (Sweden)

    Duo Teng

    2016-01-01

    Full Text Available The effective ways for underwater transducer to lower its operating frequency, to broaden its bandwidth, and to develop its miniaturization are investigated. According to the theory of coupled multimode vibrations, a novel Hybrid transducer is developed. Different from the traditional Hybrid transducer, the improved point is the low frequency vibration controlled by the zigzag piezoelectric section and the high frequency vibration controlled by the one-dimensional magnetostrictive section. Through building the equivalent circuit model and finite element model, the performances of transducer will be predicted. The analysis shows that FEM is suitable for analyzing such a Hybrid underwater transducer within 5% deviation. The corresponding tests show that the volume and weight of the Hybrid transducer undergo a sharp drop after improvement. The novel Hybrid transducer has a distinct advantage in low frequency, bandwidth, and miniaturization. The prototype has the resonance at 1.82 kHz and 3.76 kHz. It can be used effectively in the bandwidth of 1.5 kHz to 5 kHz. Its main body has an external diameter of 54 mm. The whole prototype is 235 mm long and weighs 2.61 kg.

  9. Ultrasound Doppler measurements inside a diaphragm valve using novel transducer technologies

    Science.gov (United States)

    Kotzé, Reinhardt; Wiklund, Johan

    2014-10-01

    In this project, velocity profiles were measured in a diaphragm valve using an ultrasonic velocity profiling (UVP) technique. A non-Newtonian CMC model fluid was tested in this highly complex geometry and velocity profiles were measured at four different positions at the centre (contraction) of a specially manufactured 50% open diaphragm valve. The coordinates of the complex geometry and velocity magnitudes were analysed and compared to the bulk flow rate measured using an electromagnetic flow meter. Two different ultrasonic transducers (standard and delay line) were used and results were compared in order to assess velocity data close to wall interfaces as well as the accuracy and magnitude of measured velocities. The difference between calculated and measured flow rates was 32% when using the standard ultrasonic transducers. The error difference decreased to 18% when delay line transducers were introduced to the measurements. The velocity data obtained in the diaphragm valve showed a significant improvement close to the wall interfaces when using the delay line transducers. The main limitation when using delay line transducers is that beam refraction can significantly complicate measurements in a highly complex geometry such as a diaphragm valve. A new delay line transducer with no beam refraction could provide a solution. The introduction of delay line transducers showed that UVP can be used as a powerful tool for detailed flow behaviour measurements in complex geometries.

  10. Language Model Combination and Adaptation Using Weighted Finite State Transducers

    Science.gov (United States)

    Liu, X.; Gales, M. J. F.; Hieronymus, J. L.; Woodland, P. C.

    2010-01-01

    In speech recognition systems language model (LMs) are often constructed by training and combining multiple n-gram models. They can be either used to represent different genres or tasks found in diverse text sources, or capture stochastic properties of different linguistic symbol sequences, for example, syllables and words. Unsupervised LM adaption may also be used to further improve robustness to varying styles or tasks. When using these techniques, extensive software changes are often required. In this paper an alternative and more general approach based on weighted finite state transducers (WFSTs) is investigated for LM combination and adaptation. As it is entirely based on well-defined WFST operations, minimum change to decoding tools is needed. A wide range of LM combination configurations can be flexibly supported. An efficient on-the-fly WFST decoding algorithm is also proposed. Significant error rate gains of 7.3% relative were obtained on a state-of-the-art broadcast audio recognition task using a history dependently adapted multi-level LM modelling both syllable and word sequences

  11. Multiaxial motorcycle wheel load transducer; Mehrkomponenten-Motorradmessnabe

    Energy Technology Data Exchange (ETDEWEB)

    Kuchler, M.; Schrupp, R. [BMW AG, Muenchen (Germany)

    2001-07-01

    Knowing the loads between road and wheel is one of the elementary inputs during the virtual CAE-based motorcycle design process and the real hardware test in the laboratory and on the track. Up to now, load measurements have been done by expensive strain gage applications on chassis components of the motorcycle, which forced extensive and time-wasting calibrations and calculations. The solution to that problem was the development of the worldwide unique multiaxial wheel load transducer for motorcycles, a joint project of the fatigue strength division and the motorcycle measuring division. (orig.) [German] Die Kenntnis der Kraefte zwischen Rad und Strasse ist eine der wesentlichen Eingangsgroessen sowohl fuer den CAE-gestuetzten virtuellen Produktentstehungsprozess als auch fuer die Versuche im Labor und auf der Teststrecke. In der Vergangenheit wurden die Lasten im Fahrbetrieb durch teuere Dehnungsmessstreifen-Applikationen an Fahrwerksbauteilen ermittelt, die umfangreiche und zeitaufwaendige Kalibrierungen und Signalverrechnungen erforderten. Die Loesung dieses Problems war die Entwicklung der weltweit ersten Mehrkomponenten-Motorradmessnabe, einem Gemeinschaftsprojekt der Abteilung Betriebsfestigkeit und der Motorradmesstechnik. (orig.)

  12. Electronic Current Transducer (ECT) for high voltage dc lines

    Science.gov (United States)

    Houston, J. M.; Peters, P. H., Jr.; Summerayes, H. R., Jr.; Carlson, G. J.; Itani, A. M.

    1980-02-01

    The development of a bipolar electronic current transducer (ECT) for measuring the current in a high voltage dc (HVDC) power line at line potential is discussed. The design and construction of a free standing ECT for use on a 400 kV line having a nominal line current of 2000 A is described. Line current is measured by a 0.0001 ohm shunt whose voltage output is sampled by a 14 bit digital data link. The high voltage interface between line and ground is traversed by optical fibers which carry digital light signals as far as 300 m to a control room where the digital signal is converted back to an analog representation of the shunt voltage. Two redundant electronic and optical data links are used in the prototype. Power to operate digital and optical electronics and temperature controlling heaters at the line is supplied by a resistively and capacitively graded 10 stage cascade of ferrite core transformers located inside the hollow, SF6 filled, porcelain support insulator. The cascade is driven by a silicon controlled rectifier inverter which supplies about 100 W of power at 30 kHz.

  13. Modal characterization of composite flat plate models using piezoelectric transducers

    Science.gov (United States)

    Oliveira, É. L.; Maia, N. M. M.; Marto, A. G.; da Silva, R. G. A.; Afonso, F. J.; Suleman, A.

    2016-10-01

    This paper aims to estimate the modal parameters of composite flat plate models through Experimental Modal Analysis (EMA) using piezoelectric transducers. The flat plates are composed of three ply carbon-epoxy fibers oriented in the same direction. Five specimens with different unidirectional fiber nominal orientations θk (0o, 30o, 45o, 60o and 90o) were tested. These models were instrumented with one PZT (Lead Zirconate Titanate) actuator and one PVDF (Polyvinylidene Fluoride) sensor and an EMA was performed. The natural frequencies and damping factors estimated using only a single PVDF response were compared with the estimated results using twelve measurement points acquired by laser doppler vibrometry. For comparison purposes, the percentage error of each natural frequency estimation and the percentage error of the damping factor estimations were computed, as well as their averages. Even though the comparison was made between a SISO (Single-Input, Single-Output) and a SIMO (Single-Input, Multiple-Output) techniques, both results are very close. The vibration modes were estimated by means of laser measurements and were used in the modal validation. In order to verify the accuracy of the modal parameters, the Modal Assurance Criterion (MAC) was employed and a high correlation among mode shapes was observed.

  14. Simulation of the transient electromechanical behaviour of dielectric elastomer transducers

    Science.gov (United States)

    Mößinger, Holger; Förster-Zügel, Florentine; Schlaak, Helmut F.

    2016-04-01

    To design systems utilizing dielectric elastomer transducers (DET) models are necessary to describe the behaviour of the DET and assess the system performance in advance. For basic set-ups simple analytical models or lumped parameter models are available and provide reasonable results. For more complex set-ups these models only allow a rough estimation of the system performance, not accurate enough to achieve an optimal system design. Therefore system designers typically resort to numerical simulation tools. Commercially available tools and models specialize on either electrical or mechanical domain thus simplifying or even neglecting effects in the other domain respectively. In this work we present a simulation tool taking into account the transient electrical and mechanical behaviour of DET under different mechanical load conditions and electrical driving frequencies. Our model can describe transient electrical and mechanical behaviour, such as electrical resistance, mechanical hyperelastic and viscosity of the electrodes and dielectric material. Model parameters are derived from measurements of the dielectric and the electrode resistance as well as e.g. the materials Young's modulus. The results from the simulation are compared to simple lumped parameter based models.

  15. The Evolution of Energy-Transducing Systems. Studies with Archaebacteria

    Science.gov (United States)

    Stan-Lotter, Helga

    1996-01-01

    The dicyclohexyl carbodiimide (DCCD)- binding site of the membrane ATPase from Halobacterium saccharovorum was investigated during earlier periods of this Cooperative Agreement and was localized to a cyanogen bromide fragment of subunit 2 from amino acids 379 (Glu) to 442 (Met). Although the exact position of the reactive amino acid (probably a glutamic acid) has not yet been determined, the data, together with recently obtained immuno reactions and sequences of Cyanogen Bromide (CNBr) fragments from E.coli F-ATPase, suggested subunit interactions in the halobacterial ATPase which had not been recognized before. They also provided evidence for the presence of a gamma subunit in the halobacterial ATPase, and for a stretch of a amino acids similar to the 'catch' between beta and gamma in bovine F-ATPase. The evolutionary implications of these findings are twofold: first, halobacterial (or archaebacterial) ATPases appear as complex as those from higher organisms - no simpler versions of these membrane enzymes are known to date; second, a monophyletic origin of the energy-transducing ATPases is becoming more apparent, and - together with other data - the split into V- and F-ATPases may have occurred much later than had been previously thought (i.e., after the split into Archaea and Bacteria). Other work included the characterization of an extremely halophilic isolate (Halococcus salifodinae ) from Permian salt sediments. This organism appeared to be an autotrophic halobacterium; its incorporation of C02 was investigated.

  16. Damage Identification of Wind Turbine Blades Using Piezoelectric Transducers

    Directory of Open Access Journals (Sweden)

    Seong-Won Choi

    2014-01-01

    Full Text Available This paper presents the experimental results of active-sensing structural health monitoring (SHM techniques, which utilize piezoelectric transducers as sensors and actuators, for determining the structural integrity of wind turbine blades. Specifically, Lamb wave propagations and frequency response functions at high frequency ranges are used to estimate the condition of wind turbine blades. For experiments, a 1 m section of a CX-100 blade is used. The goal of this study is to assess and compare the performance of each method in identifying incipient damage with a consideration given to field deployability. Overall, these methods yielded a sufficient damage detection capability to warrant further investigation. This paper also summarizes the SHM results of a full-scale fatigue test of a 9 m CX-100 blade using piezoelectric active sensors. This paper outlines considerations needed to design such SHM systems, experimental procedures and results, and additional issues that can be used as guidelines for future investigations.

  17. Study on heavy matching layer transducer towards producing second harmonics

    Science.gov (United States)

    Zaini, Zulfadhli; Osuga, Masamizu; Jimbo, Hayato; Yasuda, Jun; Takagi, Ryo; Yoshizawa, Shin; Umemura, Shin-ichiro

    2016-07-01

    Cavitation bubbles are microbubbles which can be incepted by highly negative pressure. Producing such highly negative pressure exceeding the cavitation threshold is difficult to accomplish due to nonlinear propagation followed by focal phase shift. By superimposing the second harmonic to fundamental is a way to significantly reduce the problem. However, the conventional design for an air-backed transducer is not suitable to generate both the second harmonic and fundamental at the same time. In order to overcome this problem, we propose a high impedance matching layer approach. Furthermore, we also construct a study to foresee the impact by adjusting the thickness ratio towards fundamental and second harmonic. Numerical simulation and experimental measurement have shown that by using a high impedance matching layer, efficiently generation of both the second harmonic (2 MHz) and fundamental (1 MHz) at the same time is possible. Besides, by adjusting the thickness ratio between piezocomposite and heavy matching layer will influence the amplitude of acoustic power over squared of voltage of the fundamental and second harmonic.

  18. Multiple facets of tightly coupled transducer-transistor structures

    Science.gov (United States)

    Heidari, Hadi; Dahiya, Ravinder

    2015-12-01

    The ever increasing demand for data processing requires different paradigms for electronics. Excellent performance capabilities such as low power and high speed in electronics can be attained through several factors including using functional materials, which sometimes acquire superior electronic properties. The transduction-based transistor switching mechanism is one such possibility, which exploits the change in electrical properties of the transducer as a function of a mechanically induced deformation. Originally developed for deformation sensors, the technique is now moving to the centre stage of the electronic industry as the basis for new transistor concepts to circumvent the gate voltage bottleneck in transistor miniaturization. In issue 37 of Nanotechnology, Chang et al show the piezoelectronic transistor (PET), which uses a fast, low-power mechanical transduction mechanism to propagate an input gate voltage signal into an output resistance modulation. The findings by Chang et al will spur further research into piezoelectric scaling, and the PET fabrication techniques needed to advance this type of device in the future.

  19. Bio-applications of ionic polymer metal composite transducers

    Science.gov (United States)

    Aw, K. C.; McDaid, A. J.

    2014-07-01

    Traditional robotic actuators have advanced performance which in some aspects can surpass that of humans, however they are lacking when it comes to developing devices which are capable of operating together with humans. Bio-inspired transducers, for example ionic polymer metal composites (IPMC), which have similar properties to human tissue and muscle, demonstrate much future promise as candidates for replacing traditional robotic actuators in medical robotics applications. This paper outlines four biomedical robotics applications, an IPMC stepper motor, an assistive glove exoskeleton/prosthetic hand, a surgical robotic tool and a micromanipulation system. These applications have been developed using mechanical design/modelling techniques with IPMC ‘artificial muscle’ as the actuation system. The systems are designed by first simulating the performance using an IPMC model and dynamic models of the mechanical system; the appropriate advanced adaptive control schemes are then implemented to ensure that the IPMCs operate in the correct manner, robustly over time. This paper serves as an overview of the applications and concludes with some discussion on the future challenges of developing real-world IPMC applications.

  20. Singulation for imaging ring arrays of capacitive micromachined ultrasonic transducers

    International Nuclear Information System (INIS)

    Singulation of MEMS is a critical step in the transition from wafer-level to die-level devices. As is the case for capacitive micromachined ultrasound transducer (CMUT) ring arrays, an ideal singulation must protect the fragile membranes from the processing environment while maintaining a ring array geometry. The singulation process presented in this paper involves bonding a trench-patterned CMUT wafer onto a support wafer, deep reactive ion etching (DRIE) of the trenches, separating the CMUT wafer from the support wafer and de-tethering the CMUT device from the CMUT wafer. The CMUT arrays fabricated and singulated in this process were ring-shaped arrays, with inner and outer diameters of 5 mm and 10 mm, respectively. The fabricated CMUT ring arrays demonstrate the ability of this method to successfully and safely singulate the ring arrays and is applicable to any arbitrary 2D shaped MEMS device with uspended microstructures, taking advantage of the inherent planar attributes of DRIE. (technical note)

  1. Resonant gravimetric immunosensing based on capacitive micromachined ultrasound transducers

    KAUST Repository

    Viržonis, Darius

    2014-04-08

    High-frequency (40 MHz) and low-frequency (7 MHz) capacitive micromachined ultrasound transducers (CMUT) were fabricated and tested for use in gravimetric detection of biomolecules. The low-frequency CMUT sensors have a gold-coated surface, while the high-frequency sensors have a silicon nitride surface. Both surfaces were functionalized with bovine leukemia virus antigen gp51 acting as the antigen. On addition of an a specific antibody labeled with horseradish peroxidase (HRP), the antigen/antibody complex is formed on the surface and quantified by HRP-catalyzed oxidation of tetramethylbenzidine. It has been found that a considerably smaller quantity of immuno complex is formed on the high frequency sensor surface. In parallel, the loading of the surface of the CMUT was determined via resonance frequency and electromechanical resistance readings. Following the formation of the immuno complexes, the resonance frequencies of the low-frequency and high-frequency sensors decrease by up to 420 and 440 kHz, respectively. Finite element analysis reveals that the loading of the (gold-coated) low frequency sensors is several times larger than that on high frequency sensors. The formation of the protein film with pronounced elasticity and stress on the gold surface case is discussed. We also discuss the adoption of this method for the detection of DNA using a hybridization assay following polymerase chain reaction.

  2. Development of capacitive micromachined ultrasonic transducer for noncontact ultrasonic detection

    International Nuclear Information System (INIS)

    In this study, the capacitive micromachined ultrasonic transducer (cMUT) was developed. Theoretical analysis and finite element analysis of the behavior of membrane (such as resonance frequency, membrane deflection, collapse deflection and collapse voltage) of the cMUT were performed. The design parameters of the cMUT were estimated and are the dimension and thickness of membrane, thickness of sacrificial layer, thickness and size of electrode, size of active element and so on. With the micro-fabrication process, the cMUT was fabricated on the silicon wafer. To measure the membrane displacement of cMUT, the Michelson phase modulation fiber interferometer was constructed. The measured membrane displacement was good agreed with the result of finite element analysis. To estimate the ultrasonic wave generated by the cMUT, the ultrasonic transceiver system was constructed. The developed cMUT shows a good performance and hence will be widely used to the filed of non-contact ultrasonic application.

  3. Phosphatase of regenerating liver-3-signal transducer and activators of transcription 3-miR-21 sighal pathway promotes proliferation and invasion of colon cancer cells%肝再生磷酸酶-3-信号传导和转录激活子3-微小RNA21信号通路促进结肠癌细胞增殖侵袭的研究

    Institute of Scientific and Technical Information of China (English)

    张建龙; 张育超; 孙健; 何传超; 褚忠华

    2012-01-01

    目的 观察肝再生磷酸酶-3-信号传导和转录激活子3-微小RNA21(PRL-3-STAT3miR-21)信号通路对结肠癌细胞增殖侵袭的作用.方法 构建稳定转染PRL-3基因和空白对照质粒的结肠癌细胞株LoVo-PRL-3和LoVo-VC,用荧光实时定量聚合酶链反应(RT-qPCR)检测稳转细胞株中miR-21的表达并在瞬时转染PRL-3的SW480及CaCO2细胞中进行验证.用Western blot法对PRL-3调控STAT3的表达进行检测,在LoVo-PRL-3细胞中对STAT3进行RNA干扰,检测miR-21的表达,在稳转细胞株中转染miR-21或对其进行敲除,用细胞计数试剂盒(CCK-8)、Transwell实验对细胞的增殖侵袭能力的变化进行研究.结果 LoVo-PRL-3细胞在72、96 h的增殖能力以及在Transwell实验24 h后的侵袭能力要强于对照组LoVo-VC细胞(P<0.01).在结肠癌细胞株LoVo-PRL-3中pSTAT3、miR-21的表达明显上调,干扰STAT3可以抑制miR-21的表达(P<0.05).在LoVo-VC细胞中过表达miR-21促进了细胞的增殖侵袭(P<0.01),而在LoVo-PRL-3细胞中敲除miR-21抑制了细胞的增殖侵袭(P<0.05).结论 PRL-3-STAT3-miR-21信号通路在结肠癌细胞增殖侵袭中起促进作用.%Objective To explore the effect of phosphatase of regenerating liver-3-signal transducer and activators of transcription 3-microRNA-21 (PRL-3-STAT3-miR-21) signal pathway on proliferation and invasion of colon cancer cellsMethods We stablely transfected PRL-3 expressing plasmid and empty plasmid into LoVo colon cancer cells and established two cell lines:LoVo-PRL-3 and LoVo-VC.We used quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) to detect the expression of miR-21 in LoVo cells.We also performed transient transfection of PRL-3 into SW480 and CACO2 cells to validate the expression of miR-21.Western blotting was used to detect the effect of PRL-3 on the expression of STAT3.RNA interference was used to knocked down STAT3 in LoVo-PRL-3 cells,after that miR-21 expression was

  4. Gene transfer and expression of enhanced green fluorescent protein in variant HT-29c cells

    Institute of Scientific and Technical Information of China (English)

    Min Wang; Lars Boenicke; Bradley D. Howard; Ilka Vogel; Hoiger Kalthoff

    2003-01-01

    AIM: To study the expression of enhanced green fluorescent protein (EGFP) gene in retrovirally transduced variant HT29 cells.METHODS: The retroviral vector prkat EGFP/neo was constructed and transfected into the 293T cell using a standard calcium phosphate precipitation method. HT-29c cells (selected from HT-29 cells) were transduced by a retroviral vector encoding the GEFP gene. The fluorescence intensity of colorectal carcinoma HT-29c cells after transduced with the EGFP bearing retrovirus was visualized using fluorescence microscope and fluorescence activated cell sorter (FACS) analysis. Multiple biological behaviors of transduced cells such as the proliferating potential and the expression of various antigens were comparatively analyzed between untransduced and transduced cells in vitro. EGFP expression of the fresh tumor tissue was assessed in vivo.RESULTS: After transduced, HT-29c cells displayed a stable and long-term EGFP expression under the nonselective conditionsin vitro. After cells were successively cultured to passage 50 in vitro, EGFP expression was still at a high level. Their biological behaviors, such as expression of tumor antigens, proliferation rate and aggregation capability were not different compared to untransduced parental cells in vitro. In subcutaneous tumors, EGFP was stable and highly expressed.CONCLUSION: An EGFP expressing retroviral vector was used to transduce HT-29c cells. The transduced cells show a stable and long-term EGFP expression in vitro and in vivo.These cells with EGFP are a valuable tool forin vivo research of tumor metastatic spread.

  5. Free Field Reciprocity Calibration in a Convergent Spherical Acoustic Wave of a Focusing Transducer

    Institute of Scientific and Technical Information of China (English)

    寿文德; 严加勇; 王鸿樟; 钱德初

    2002-01-01

    Based on the reciprocity theorem of the acoustic field, we derive the formula of the reciprocity coefficient of a convergent spherical acoustic wave and we calculate a series of diffraction corrective factor curves of the reciprocity coefficient of transducers. Using these formulae and corrective factors, we calibrate the free field transmitting current response and the free field voltage sensitivity of a focusing transducer using the self-reciprocity method.The experimental results of the reciprocity calibration of the focusing transducer in the frequency range of 2 MHz to 5.4 MHz are presented.

  6. Development of high-temperature ultrasonic transducers for under-sodium viewing applications

    International Nuclear Information System (INIS)

    PZT type immersion transducers, bonded using pure lead, can be manufactured and operated satisfactorily in sodium at temperatures up to 2800C. A satisfactory technique of braze bonding lithium niobate Z-cut crystal to stainless steel diaphragms has been developed. The bonded diaphragms can be assembled into transducers which are then capable of operation at temperatures up to 6000C. Sealed transducers which had been pre-oxidised, maintained sensitivity for about 70 days when operated at temperatures between 3000C and 5000C. There was subsequently a slow fall in sensitivity with time at temperature. (Author)

  7. Design and fabrication of PIN-PMN-PT single-crystal high-frequency ultrasound transducers.

    Science.gov (United States)

    Sun, Ping; Zhou, Qifa; Zhu, Benpeng; Wu, Dawei; Hu, Changhong; Cannata, Jonathan M; Tian, Jin; Han, Pengdi; Wang, Gaofeng; Shung, K Kirk

    2009-12-01

    High-frequency PIN-PMN-PT single crystal ultrasound transducers at center frequencies of 35 MHz and 60 MHz were successfully fabricated using lead indium niobate-lead magnesium niobate-lead titanate (0.23PIN- 0.5PMN-0.27PT) single crystal. The new PIN-PMN-PT single crystal has higher coercivity (6.0 kV/cm) and higher Curie temperature (160 degrees C) than PMN-PT crystal. Experimental results showed that the PIN-PMN-PT transducers have similar performance but better thermal stability compared with the PMN-PT transducers. PMID:20040413

  8. Development of ultrasonic transducer with high resistance to temperature and radiation

    International Nuclear Information System (INIS)

    Ordinary ultrasonic transducers cannot be used to inspect equipment at a high radiation facility such as vitrification facility or fusion reactor. IHI has developed an ultrasonic transducer with high resistance to temperature and radiation and confirmed the radiation tolerance up to 10 MGy. IHI has applied the ultrasonic transducer to lid welding inspection of vitrification packages, and obtained good results such as clear detection of an artificial 0.5 mm diameter hole penetrating the stainless steel lid welding. This technology could be applied to ISI (In-Service Inspection) of nuclear plants, ensuring safe operation of nuclear plants. (author)

  9. Effect of Thermal Degradation on High Temperature Ultrasonic Transducer Performance in Small Modular Reactors

    Science.gov (United States)

    Bilgunde, Prathamesh N.; Bond, Leonard J.

    Prototype ultrasonic NDT transducers for use in immersion in coolants for small modular reactors have shown low signal to noise ratio. The reasons for the limitations in performance at high temperature are under investigation, and include changes in component properties. This current work seeks to quantify the issue of thermal expansion and degradation of the piezoelectric material in a transducer using a finite element method. The computational model represents an experimental set up for an ultrasonic transducer in a pulse-echo mode immersed in a liquid sodium coolant. Effect on transmitted and received ultrasonic signal due to elevated temperature (∼200oC) has been analysed.

  10. A lightweight push-pull acoustic transducer composed of a pair of dielectric elastomer films.

    Science.gov (United States)

    Sugimoto, Takehiro; Ando, Akio; Ono, Kazuho; Morita, Yuichi; Hosoda, Kosuke; Ishii, Daisaku; Nakamura, Kentaro

    2013-11-01

    A lightweight push-pull acoustic transducer using dielectric elastomer films was proposed for use in advanced audio systems in homes. The push-pull structure consists of two dielectric elastomer films developed to serve as an electroactive polymer. The transducer utilizes the change in the surface area of the dielectric elastomer film, induced by an electric-field-induced change in the thickness, for sound generation. The resonance frequency of the transducer was derived from modeling the push-pull configuration to estimate the lower limit of the frequency range. Measurement results presented an advantage of push-pull driving in the suppression of harmonic distortion. PMID:24181987

  11. Fluctuating surface pressure measurements on USB wing using two types of transducers

    Science.gov (United States)

    Reed, J. B.

    1975-01-01

    Measurements of the fluctuating pressures on the wing surface of an upper-surface-blown powered-lift model and a JT15 engine were obtained using two types of pressure transducers. The pressures were measured using overall-fluctuating pressures and power spectral density analyses for various thrust settings and two jet impingement angles. Comparison of the data from the two transducers indicate that similar results are obtained in the lower frequency ranges for both transducers. The data also indicate that for this configuration, the highest pressure levels occur at frequencies below 2000 Hz.

  12. Design of sonar transducer based on the laser-induced sound

    Institute of Scientific and Technical Information of China (English)

    WANG Yuhong; WANG Jiangan; ZONG Siguang; WU Ronghua

    2009-01-01

    A design of a kind of sonar transducers was presented. An experimental system was developed for producing the sound signal based on the laser-induced breakdown and bubble oscillation. The power spectrum and the ambiguity function were analyzed. The range resolu-tion and the Doppler resolution constant of the sound signal were determined. It is basically proved in theory that both the range resolution and directivity of the sonar transducer are very high. The transducer can be a sound source of high-resolution sonars.

  13. Reliability and Validity Assessment of a Linear Position Transducer

    Directory of Open Access Journals (Sweden)

    Manuel V. Garnacho-Castaño

    2015-03-01

    Full Text Available The objectives of the study were to determine the validity and reliability of peak velocity (PV, average velocity (AV, peak power (PP and average power (AP measurements were made using a linear position transducer. Validity was assessed by comparing measurements simultaneously obtained using the Tendo Weightlifting Analyzer Systemi and T-Force Dynamic Measurement Systemr (Ergotech, Murcia, Spain during two resistance exercises, bench press (BP and full back squat (BS, performed by 71 trained male subjects. For the reliability study, a further 32 men completed both lifts using the Tendo Weightlifting Analyzer Systemz in two identical testing sessions one week apart (session 1 vs. session 2. Intraclass correlation coefficients (ICCs indicating the validity of the Tendo Weightlifting Analyzer Systemi were high, with values ranging from 0.853 to 0.989. Systematic biases and random errors were low to moderate for almost all variables, being higher in the case of PP (bias ±157.56 W; error ±131.84 W. Proportional biases were identified for almost all variables. Test-retest reliability was strong with ICCs ranging from 0.922 to 0.988. Reliability results also showed minimal systematic biases and random errors, which were only significant for PP (bias -19.19 W; error ±67.57 W. Only PV recorded in the BS showed no significant proportional bias. The Tendo Weightlifting Analyzer Systemi emerged as a reliable system for measuring movement velocity and estimating power in resistance exercises. The low biases and random errors observed here (mainly AV, AP make this device a useful tool for monitoring resistance training.

  14. Alternative material study for heat assisted magnetic recording transducer application

    Science.gov (United States)

    Xu, B. X.; Cen, Z. H.; Hu, J. F.; Tsai, J. W. H.

    2015-05-01

    In heat assisted magnetic recording (HAMR), optical near field transducer (NFT) is a key component. Au is currently used as NFT material because of its strong surface plasmon effect. Due to the soft property of Au material, reliability of Au NFT becomes a key issue for realizing HAMR production. In this paper, the possibility of alternative materials, including transition metal nitrides (TMNs) and transparent conducting oxides (TCOs) to replace Au is studied. The results show that all of the listed TMN and TCO materials can meet the mechanical requirements at room temperature in terms of hardness and thermal expansion. An optical model, which includes optical waveguide, NFT and FePt media, is used to simulate NFT performances. The results indicate that the resonant wavelengths for NFT with TCO materials are longer than 1500 nm, which is not suitable for HAMR application. TMN materials are suitable for NFT application at wavelength band of around 800 nm. But the NFT efficiency is very low. ZrN is the best material among TMN materials and the efficiency of ZrN NFT is only 13% of the Au NFT's efficiency. Reducing refractive index (n) and increasing extinction coefficient (k) will both lead to efficiency increase. Increasing k contributes more in the efficiency increase, while reducing n has a relatively low NFT absorption. For materials with the same figure of merit, the NFT with larger k material has higher efficiency. Doping materials to increase the material conduction electron density and growing film with larger size grain may be the way to increase k and reduce n.

  15. Dynamic mode tuning of ultrasonic guided wave using an array transducer

    International Nuclear Information System (INIS)

    Ultrasonic guided waves have been widely employed for the long range inspection of structures such as plates, rods and pipes. In ultrasonic guided waves, however, there are numerous modes with different wave velocities, so that the generation and detection of the appropriate wave mode of the guided wave is one of key techniques in the application of guided waves. In the present work, phase tuning using an array transducer was applied to generate ultrasonic guided waves in a seamless stainless steel pipe. for this purpose, 8-channel ultrasonic pulser/receiver and their controller which enables sequential activation of each channels with given time delay were developed. Eight transducers were fabricated in order to generate guided waves by using an array transducer. The selective tuning of wave mode can be achieved by changing the interval between elements of an array transducer.

  16. Thickness effects of the metallic and the insulating membranes of a cylindrical electromagnetic shock wave transducer

    International Nuclear Information System (INIS)

    The study was to experimentally investigate how shock wave production was related to the thickness of the metallic and the insulating membranes encompassing the solenoid of a cylindrical electromagnetic shock wave transducer. The thicknesses considered were 30, 50, 100, 150, and 200 μm in the metallic foil, and 50, 100, 150, 200, 300, and 500 μm in the insulator. A total of 30 shock wave transducers covering all thickness combinations of the pair of membranes were constructed and tested. The results showed that the shock wave production was maximized when the metallic foil had a particular thickness of 50 μm and the insulator was as thin as possible. The conditions are that the inductance of the shock wave transducer is close to the lower bound of its minimum and that the current flow through the transducer is slightly smaller than its maximum.

  17. Actuatable capacitive transducer for quantitative nanoindentation combined with transmission electron microscopy

    Science.gov (United States)

    Warren, Oden L.; Asif, S. A. Syed; Cyrankowski, Edward; Kounev, Kalin

    2010-09-21

    An actuatable capacitive transducer including a transducer body, a first capacitor including a displaceable electrode and electrically configured as an electrostatic actuator, and a second capacitor including a displaceable electrode and electrically configured as a capacitive displacement sensor, wherein the second capacitor comprises a multi-plate capacitor. The actuatable capacitive transducer further includes a coupling shaft configured to mechanically couple the displaceable electrode of the first capacitor to the displaceable electrode of the second capacitor to form a displaceable electrode unit which is displaceable relative to the transducer body, and an electrically-conductive indenter mechanically coupled to the coupling shaft so as to be displaceable in unison with the displaceable electrode unit.-

  18. Driving frequency optimization of a piezoelectric transducer and the power supply development

    Science.gov (United States)

    Dong, Xiaoxiao; Yuan, Tao; Hu, Minqiang; Shekhani, Husain; Maida, Yuichi; Tou, Tonshaku; Uchino, Kenji

    2016-10-01

    Piezoelectric transducers are commonly operated at their resonance frequency. However, from a power dissipation standpoint, this is not the ideal driving frequency. In this paper, an optimized driving frequency in between the resonance and antiresonance frequencies is proposed for the piezo-transducer. First, the optimum driving frequency is characterized using a constant vibration velocity measurement method. The actual input power reveals the lowest power dissipation frequency between the resonance and the antiresonance frequencies, where the transducer behaves inductive. The electrical parameters of the transducer are then determined by an equivalent circuit formulation, which is useful for the electrical circuit analysis of the driver design. A Class E resonant inverter is used to design a capacitive output impedance driver at the optimized frequency by utilizing a series capacitor. Compared with the traditional resonance drive, driving at the optimized frequency reduces the required power by approximately half according to the measurements performed.

  19. Investigation of An Acoustic Temperature Transducer and its Application for Heater Temperature Measurement

    Directory of Open Access Journals (Sweden)

    Mohammad A.K. Alia

    2007-01-01

    Full Text Available Recent developments in temperature measurement have encouraged researchers to develop low-cost, simple structure, computerized generic transducers for environmental monitoring and industrial process control. The research presents a computerized technique which allows to measure temperature according to the variation of acoustic velocity (frequency in a closed waveguide. Signal conditioning and processing was carried out using labVIEW (G Language VIs. In order to evaluate the time characteristic of the transducer its response was compared with that of a reference detector (PT 100 for the same step input. Static characteristics of the transducer show a quasi-linear relationship between the measured temperature and the resonance frequency. Results of practical experiments show that in order to improve the response curve of the transducer and decrease the rising time interval it is advisable to implement thin-wall glass tubes or another material with lower thermal impedance.

  20. Analysis and design of piezocomposite ultrasonic transducers using finite element technique and surface displacement profiles

    CERN Document Server

    Reynolds, P

    2000-01-01

    Ultrasonic transducers have found extensive applications in the fields of non-destructive testing, biomedicine, and SONAR. Piezocomposite ultrasonic transducers can offer significant advantages over their pure ceramic counterparts, but at the expense of increased manufacturing complexity and the introduction of additional resonant modes that may reduce transducer efficiency if the device is not carefully designed. Extensive work has been carried out over the last twenty years to characterise the behaviour of piezocomposite devices, resulting in many design guidelines, some of which are only applicable in a limited range of device structures. This Thesis presents a new theory of the generation of inter-pillar modes that is based upon the generation of Lamb waves in the piezocomposite plate. Through the use of finite element analysis and a scanning laser interferometer, the resonant mode displacement shapes of piezocomposite transducers are studied and analysed. Excellent correlation between modelled and experi...