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Sample records for cell biologist don

  1. Human radiation studies: Remembering the early years: Oral history of cell biologist Don Francis Petersen, Ph.D., conducted November 29, 1994

    International Nuclear Information System (INIS)

    This report is a transcript of an interview of Dr. Don Francis Petersen by representatives of the US DOE Office of Human Radiation Experiments. Dr. Petersen was selected for this interview because of his long research career at Los Alamos and his knowledge of the Atomic Energy Commission's biomedical program. Dr. Petersen did not personally conduct research on human subjects. After a brief biographical sketch Dr. Petersen discusses his remembrances of the early use of radionuclides as biological tracers, aspects of nuclear weapons testing in the 1940's and 1950's including fallout studies, the means by which research projects were approved, use of humans in the whole-body counter, and the Health Division Biomedical responsibilities

  2. Human radiation studies: Remembering the early years: Oral history of cell biologist Don Francis Petersen, Ph.D., conducted November 29, 1994

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-08-01

    This report is a transcript of an interview of Dr. Don Francis Petersen by representatives of the US DOE Office of Human Radiation Experiments. Dr. Petersen was selected for this interview because of his long research career at Los Alamos and his knowledge of the Atomic Energy Commission`s biomedical program. Dr. Petersen did not personally conduct research on human subjects. After a brief biographical sketch Dr. Petersen discusses his remembrances of the early use of radionuclides as biological tracers, aspects of nuclear weapons testing in the 1940`s and 1950`s including fallout studies, the means by which research projects were approved, use of humans in the whole-body counter, and the Health Division Biomedical responsibilities.

  3. Thousands of rab GTPases for the cell biologist.

    Directory of Open Access Journals (Sweden)

    Yoan Diekmann

    2011-10-01

    Full Text Available Rab proteins are small GTPases that act as essential regulators of vesicular trafficking. 44 subfamilies are known in humans, performing specific sets of functions at distinct subcellular localisations and tissues. Rab function is conserved even amongst distant orthologs. Hence, the annotation of Rabs yields functional predictions about the cell biology of trafficking. So far, annotating Rabs has been a laborious manual task not feasible for current and future genomic output of deep sequencing technologies. We developed, validated and benchmarked the Rabifier, an automated bioinformatic pipeline for the identification and classification of Rabs, which achieves up to 90% classification accuracy. We cataloged roughly 8.000 Rabs from 247 genomes covering the entire eukaryotic tree. The full Rab database and a web tool implementing the pipeline are publicly available at www.RabDB.org. For the first time, we describe and analyse the evolution of Rabs in a dataset covering the whole eukaryotic phylogeny. We found a highly dynamic family undergoing frequent taxon-specific expansions and losses. We dated the origin of human subfamilies using phylogenetic profiling, which enlarged the Rab repertoire of the Last Eukaryotic Common Ancestor with Rab14, 32 and RabL4. Furthermore, a detailed analysis of the Choanoflagellate Monosiga brevicollis Rab family pinpointed the changes that accompanied the emergence of Metazoan multicellularity, mainly an important expansion and specialisation of the secretory pathway. Lastly, we experimentally establish tissue specificity in expression of mouse Rabs and show that neo-functionalisation best explains the emergence of new human Rab subfamilies. With the Rabifier and RabDB, we provide tools that easily allows non-bioinformaticians to integrate thousands of Rabs in their analyses. RabDB is designed to enable the cell biology community to keep pace with the increasing number of fully-sequenced genomes and change the scale

  4. Charged Particles are Prevented from Going Faster than the Speed of Light by Light Itself: A Biophysical Cell Biologist's Contribution to Physics

    OpenAIRE

    Wayne, Randy

    2011-01-01

    Investigations of living organisms have led biologists and physicians to introduce fundamental concepts, including Brownian motion, the First Law of Thermodynamics, Poiseuille's Law of fluid flow, and Fick's Law of diffusion into physics. Given the prominence of viscous forces within and around cells and the experience of identifying and quantifying such resistive forces, biophysical cell biologists have an unique perspective in discovering the viscous forces that cause moving particles to re...

  5. Frequent biphasic cellular responses of permanent fish cell cultures to deoxynivalenol (DON)

    International Nuclear Information System (INIS)

    Contamination of animal feed with mycotoxins is a major problem for fish feed mainly due to usage of contaminated ingredients for production and inappropriate storage of feed. The use of cereals for fish food production further increases the risk of a potential contamination. Potential contaminants include the mycotoxin deoxynivalenol (DON) which is synthesized by globally distributed fungi of the genus Fusarium. The toxicity of DON is well recognized in mammals. In this study, we confirm cytotoxic effects of DON in established permanent fish cell lines. We demonstrate that DON is capable of influencing the metabolic activity and cell viability in fish cells as determined by different assays to indicate possible cellular targets of this toxin. Evaluation of cell viability by measurement of membrane integrity, mitochondrial activity and lysosomal function after 24 h of exposure of fish cell lines to DON at a concentration range of 0-3000 ng ml-1 shows a biphasic effect on cells although differences in sensitivity occur. The cell lines derived from rainbow trout are particularly sensitive to DON. The focus of this study lies, furthermore, on the effects of DON at different concentrations on production of reactive oxygen species (ROS) in the different fish cell lines. The results show that DON mainly reduces ROS production in all cell lines that were used. Thus, our comparative investigations reveal that the fish cell lines show distinct species-related endpoint sensitivities that also depend on the type of tissue from which the cells were derived and the severity of exposure. - Highlights: → DON uptake by cells is not extensive. → All fish cell lines are sensitive to DON. → DON is most cytotoxic to rainbow trout cells. → Biphasic cellular responses were frequently observed. → Our results are similar to studies on mammalian cell lines.

  6. Charged Particles are Prevented from Going Faster than the Speed of Light by Light Itself: A Biophysical Cell Biologist's Contribution to Physics

    CERN Document Server

    Wayne, Randy

    2011-01-01

    Investigations of living organisms have led biologists and physicians to introduce fundamental concepts, including Brownian motion, the First Law of Thermodynamics, Poiseuille's Law of fluid flow, and Fick's Law of diffusion into physics. Given the prominence of viscous forces within and around cells and the experience of identifying and quantifying such resistive forces, biophysical cell biologists have an unique perspective in discovering the viscous forces that cause moving particles to respond to an applied force in a nonlinear manner. Using my experience as a biophysical cell biologist, I show that in any space consisting of a photon gas with a temperature above absolute zero, Doppler-shifted photons exert a velocity-dependent viscous force on moving charged particles. This viscous force prevents charged particles from exceeding the speed of light. Consequently, light itself prevents charged particles from moving faster than the speed of light. This interpretation provides a testable alternative to the i...

  7. Easy mathematics for biologists

    CERN Document Server

    Foster, Peter C

    2014-01-01

    Because elementary mathematics is vital to be able to properly design biological experiments and interpret their results. As a student of the life sciences you will only make your life harder by ignoring mathematics entirely. Equally, you do not want to spend your time struggling with complex mathematics that you will never use. This book is the perfect answer to your problems. Inside, it explains the necessary mathematics in easy-to-follow steps, introducing the basics and showing you how to apply these to biological situations. Easy Mathematics for Biologists covers the basic mathematical ideas of fractions, decimals and percentages, through ratio and proportion, exponents and logarithms, to straight line graphs, graphs that are not straight lines, and their transformation. Direct application of each of these leads to a clear understanding of biological calculations such as those involving concentrations and dilutions, changing units, pH, and linear and non-linear rates of reaction. Each chapter contains wo...

  8. Transcriptome analysis of the human T lymphocyte cell line Jurkat and human peripheral blood mononuclear cells exposed to deoxynivalenol (DON): New mechanistic insights

    Energy Technology Data Exchange (ETDEWEB)

    Katika, Madhumohan R. [RIKILT-Institute of Food Safety, Wageningen University and Research Centre, Wageningen (Netherlands); Department of Health Risk Analysis and Toxicology, Maastricht University (Netherlands); Netherlands Toxicogenomics Centre (Netherlands); Hendriksen, Peter J.M. [RIKILT-Institute of Food Safety, Wageningen University and Research Centre, Wageningen (Netherlands); Netherlands Toxicogenomics Centre (Netherlands); Shao, Jia [RIKILT-Institute of Food Safety, Wageningen University and Research Centre, Wageningen (Netherlands); Department of Health Risk Analysis and Toxicology, Maastricht University (Netherlands); Netherlands Toxicogenomics Centre (Netherlands); Loveren, Henk van [Department of Health Risk Analysis and Toxicology, Maastricht University (Netherlands); National Institute for Public Health and the Environment (RIVM), Bilthoven (Netherlands); Netherlands Toxicogenomics Centre (Netherlands); Peijnenburg, Ad, E-mail: ad.peijnenburg@wur.nl [RIKILT-Institute of Food Safety, Wageningen University and Research Centre, Wageningen (Netherlands); Netherlands Toxicogenomics Centre (Netherlands)

    2012-10-01

    Deoxynivalenol (DON) or vomitoxin is a commonly encountered type-B trichothecene mycotoxin, produced by Fusarium species predominantly found in cereals and grains. DON is known to exert toxic effects on the gastrointestinal, reproductive and neuroendocrine systems, and particularly on the immune system. Depending on dose and exposure time, it can either stimulate or suppress immune function. The main objective of this study was to obtain a deeper insight into DON-induced effects on lymphoid cells. For this, we exposed the human T-lymphocyte cell line Jurkat and human peripheral blood mononuclear cells (PBMCs) to various concentrations of DON for various times and examined gene expression changes by DNA microarray analysis. Jurkat cells were exposed to 0.25 and 0.5 μM DON for 3, 6 and 24 h. Biological interpretation of the microarray data indicated that DON affects various processes in these cells: It upregulates genes involved in ribosome structure and function, RNA/protein synthesis and processing, endoplasmic reticulum (ER) stress, calcium-mediated signaling, mitochondrial function, oxidative stress, the NFAT and NF-κB/TNF-α pathways, T cell activation and apoptosis. The effects of DON on the expression of genes involved in ER stress, NFAT activation and apoptosis were confirmed by qRT-PCR. Other biochemical experiments confirmed that DON activates calcium-dependent proteins such as calcineurin and M-calpain that are known to be involved in T cell activation and apoptosis. Induction of T cell activation was also confirmed by demonstrating that DON activates NFATC1 and induces its translocation from the cytoplasm to the nucleus. For the gene expression profiling of PBMCs, cells were exposed to 2 and 4 μM DON for 6 and 24 h. Comparison of the Jurkat microarray data with those obtained with PBMCs showed that most of the processes affected by DON in the Jurkat cell line were also affected in the PBMCs. -- Highlights: ► The human T cell line Jurkat and human

  9. Phytic acid protects porcine intestinal epithelial cells from deoxynivalenol (DON) cytotoxicity.

    OpenAIRE

    Pacheco, Graziela Drociunas; Silva, Caio Abércio da; Pinton, Philippe; Oswald, Isabelle

    2012-01-01

    The purpose of this study was to evaluate the effects of phytic acid (IP(6)) as a possible inhibitor of cellular damage induced by toxic substances such as mycotoxins on a porcine intestinal epithelial cell line (IPEC-1). We first observed that a dose of 5 mM phytic acid decreases cell viability and transepithelial electrical resistance (TEER) of cell monolayer. We next investigate the effect of non-cytotoxic dose of phytic acid on the deoxinivalenol (DON) induced decreased TEER. We showed th...

  10. A novel recombinant cell fluorescence biosensor based on toxicity of pathway for rapid and simple evaluation of DON and ZEN.

    Science.gov (United States)

    Ji, Jian; Gu, Wenshu; Sun, Chao; Sun, Jiadi; Jiang, Hui; Zhang, Yinzhi; Sun, Xiulan

    2016-01-01

    During an exposure, humans and animals are most often exposed to a mixture rather than individual mycotoxins. In this study, a Human Embryonic Kidney 293 cell (HEK-293) fluorescence sensor was developed to detect and evaluate mycotoxins, deoxynivalenol (DON) and zearalenone (ZEN) compounds, produced by Fusarium culmorum that are common food contaminants. TRE-copGFP (green fluorescent protein) and ERE-TagRFP (red fluorescent protein) plasmids were constructed and cotransfected into HEK-293 cells through a highly efficient, lipid-mediated, DNA-transfection procedure. Results show that fluorescence intensity was proportional to DON and ZEN concentrations, ranging from 2 to 40 ng/mL and 10 to 100 ng/mL respectively, with a detection limit of 0.75 ng/mL and 3.2 ng/mL respectively. The EC50 of DON and ZEN are 30.13 ng/mL and 76.63 ng/mL respectively. Additionally, ZEN may have a synergistic effect on enhancing AP-1 activity of the toxicity pathway of DON. These data indicate the high sensitivity and effectiveness of our biosensor system in the evaluation of the combined toxicity of ZEN, DON and their derivatives. In addition, this approach is suitable for an early warning method for the detection of ZEN and DON family mycotoxins contamination without higher-priced, conventional analytical chemistry methods. PMID:27498557

  11. A novel recombinant cell fluorescence biosensor based on toxicity of pathway for rapid and simple evaluation of DON and ZEN

    Science.gov (United States)

    Ji, Jian; Gu, Wenshu; Sun, Chao; Sun, Jiadi; Jiang, Hui; Zhang, Yinzhi; Sun, Xiulan

    2016-01-01

    During an exposure, humans and animals are most often exposed to a mixture rather than individual mycotoxins. In this study, a Human Embryonic Kidney 293 cell (HEK-293) fluorescence sensor was developed to detect and evaluate mycotoxins, deoxynivalenol (DON) and zearalenone (ZEN) compounds, produced by Fusarium culmorum that are common food contaminants. TRE-copGFP (green fluorescent protein) and ERE-TagRFP (red fluorescent protein) plasmids were constructed and cotransfected into HEK-293 cells through a highly efficient, lipid-mediated, DNA-transfection procedure. Results show that fluorescence intensity was proportional to DON and ZEN concentrations, ranging from 2 to 40 ng/mL and 10 to 100 ng/mL respectively, with a detection limit of 0.75 ng/mL and 3.2 ng/mL respectively. The EC50 of DON and ZEN are 30.13 ng/mL and 76.63 ng/mL respectively. Additionally, ZEN may have a synergistic effect on enhancing AP-1 activity of the toxicity pathway of DON. These data indicate the high sensitivity and effectiveness of our biosensor system in the evaluation of the combined toxicity of ZEN, DON and their derivatives. In addition, this approach is suitable for an early warning method for the detection of ZEN and DON family mycotoxins contamination without higher-priced, conventional analytical chemistry methods. PMID:27498557

  12. A chemist and biologist talk to each other about caged neurotransmitters

    OpenAIRE

    Ellis-Davies, Graham C. R.

    2013-01-01

    Caged compounds are small organic molecules that can be photoactivated with brief pulses of light. They are widely used to study a great variety of biological processes by physiologists, cell biologists and neuroscientists. Initially made and invented by biologists in the late 1970s, they are now made mostly by chemists, often without any dialogue with the end users, the biologists. The idea for this review is to stimulate interaction between the two communities to further the creative develo...

  13. Cell differentiation, secondary cell-wall formation and transformation of callus tissue of Pinus radiata D. Don.

    Science.gov (United States)

    Möller, Ralf; McDonald, Armando G; Walter, Christian; Harris, Philip J

    2003-09-01

    Tracheid and sclereid differentiation was induced in callus cultures of Pinus radiata D. Don by culturing on a basal medium containing activated charcoal but no phytohormones; sclereids differentiated in callus derived from xylem strips, but not in callus derived from hypocotyl segments. The tracheids differentiated in hypocotyl-derived callus had helical, scalariform, reticulated or pitted secondary cell-wall patterns, but those differentiated in xylem-derived callus had a reticulate or pitted pattern. The thickened tracheid and sclereid walls contained lignin as indicated by the red colour reaction given with phloroglucinol-HCl. The presence of lignin in the cell walls of differentiated callus was confirmed using pyrolysis gas chromatography-mass spectrometry by the detection of phenylpropanoid components derived from lignin. Lignin was also detected using solid-state (13)C cross-polarisation/magic-angle spinning nuclear magnetic resonance spectroscopy and quantified as thioglycolic acid lignin. Monosaccharide analyses of the cell walls isolated from differentiated and undifferentiated calli showed that the cell walls of the differentiated calli contained higher proportions of glucose and mannose, consistent with the presence of greater proportions of gluco- and/or galactogluco-mannans in the secondary cell walls of the differentiated cells. A protocol for the stable transformation of undifferentiated, xylem-derived cultures was successfully developed. Transgenic cell lines were established following Biolistic particle bombardment with a plasmid containing the coding region of the nptII gene and the coding region of the cad gene from P. radiata. Expression of the nptII gene in transgenic lines was confirmed by an NPTII-enzyme-linked immunosorbent assay. The overexpression of cad in the transgenic lines resulted in a down-regulation of cinnamyl alcohol dehydrogenase (EC 1.1.1.195) expression. PMID:12811558

  14. Effects of deoxynivalenol (DON), zearalenone (ZEN), and related metabolites on equine peripheral blood mononuclear cells (PBMC) in vitro and background occurrence of these toxins in horses.

    Science.gov (United States)

    Schumann, Barbara; Winkler, Janine; Mickenautsch, Nicola; Warnken, Tobias; Dänicke, Sven

    2016-08-01

    Both deoxynivalenol (DON), zearalenone (ZEN), and their metabolites are known to modulate immune cells in various species whereby viability and proliferation are influenced. Such effects were rarely examined in horses. Therefore, one aim of the present study was to titrate the inhibitory concentrations of DON, 3-acetyl-DON (3AcDON), de-epoxy-DON (DOM-1), ZEN, and α- and β-zearalenol (ZEL) at which viability and proliferation of equine PBMC were reduced by 50 % (IC50) and 10 % (IC10) in vitro. For evaluation of practical relevance of the in vitro findings, a further aim was to screen horses for the background occurrence of DON, ZEN, and their metabolites in systemic circulation and to relate toxin residues both to the inhibitory toxin concentrations and to hematological and clinical-chemical characteristics.The IC50 (μM) for DON, 3AcDON, β-ZEL, α-ZEL, and ZEN were determined at 3.09, 25.90, 75.44, 97.44, and 98.15 in unstimulated cells, respectively, while in proliferating cells, the corresponding IC50 values were 0.73, 6.89, 45.16, 75.96, and 82.51. Neither viability nor proliferation was influenced by DOM-1 up to a concentration of 100 μM.The in vivo screening (N = 49) revealed the occurrence of ZEN (N = 24), α-ZEL (N = 3), β-ZEL (N = 37), DON, and DOM-1 (N = 2). The detected concentrations were much lower than the corresponding IC50 while the IC10 of DON and β-ZEL for proliferating PBMC corresponded to approximately 26 and 35 ng/mL which might be relevant when contaminated diets are fed.Clinical-chemical and hematological traits were not related to mycotoxin residue levels excepting blood urea nitrogen which was positively correlated to the sum of β-ZEL, α-ZEL, and ZEN concentration. Whether this reflects simply the feeding history of the horses or renal failures giving rise to a prolonged half-life of the toxins needs to be clarified further. PMID:27255919

  15. Pharmacological activity in growth inhibition and apoptosis of cultured human leiomyomal cells of tropical plant Scutellaria barbata D. Don (Lamiaceae).

    Science.gov (United States)

    Lee, Tae-Kyun; Lee, Yun-Jeong; Kim, Dong-Il; Kim, Hyung-Min; Chang, Young-Chae; Kim, Cheorl-Ho

    2006-01-01

    Scutellaria barbata D. Don (Lamiaceae) (SB), which is known in traditional Korean medicine, has been used as an anti-inflammatory and antitumor agent. Since uterine leiomyoma is the most common benign smooth muscle cell tumor of the myometrium, we aimed to determine the growth inhibition and the induction of apoptotic cell death brought about by the herb SB in two different leiomyomal cells, named LM-1 and LM-2, and to clarify the mechanism of this apoptosis. Water-soluble ingredients of SB, and the leiomyomal cell lines of LM-1 and LM-2, were used in vitro. Growth inhibition, induction of cell death, morphological features, the presence of DNA ladders, increases in Caspase 3-like activity, the effects of a Caspase 3 inhibitor on apoptotic cell death, and the release of Cytochrome C by SB were analyzed. SB inhibited the growth and decreased the viability of the leiomyomal cells. The viability of normal myomatrial smooth muscle cells (SMC) in the presence of low concentrations of SB was higher than those of leiomyomal cells. Apoptotic bodies and DNA ladders were observed to be induced in leiomyomal cells of LM-1 and LM-2 by SB. The synthetic tetrapeptide Caspase 3 inhibitor, N-acetyl-Asp-Glu-Val-Asp-aldehyde (Ac-DEVD-CHO), inhibited the apoptotic cell death of leiomyomal cells induced by SB. The Caspase 3-like activity in leiomyomal cells LM-1 and LM-2 increased after the addition of SB. Cytochrome C was released from mitochondria into the cytosol 8h after the addition of SB, and reached a peak at 16h. The peak of Cytochrome C release was earlier than that of Caspase 3-like activity. We concluded that SB inhibited the growth of the leiomyomal cells and induced apoptosis. The apoptosis of leiomyomal cells induced by SB was associated with the release of Cytochrome C from the mitochondria, followed by an increase in Caspase 3-like activity.

  16. A chemist and biologist talk to each other about caged neurotransmitters

    Directory of Open Access Journals (Sweden)

    Graham C.R. Ellis-Davies

    2013-01-01

    Full Text Available Caged compounds are small organic molecules that can be photoactivated with brief pulses of light. They are widely used to study a great variety of biological processes by physiologists, cell biologists and neuroscientists. Initially made and invented by biologists in the late 1970s, they are now made mostly by chemists, often without any dialogue with the end users, the biologists. The idea for this review is to stimulate interaction between the two communities to further the creative development and application of these powerful optical probes.

  17. Computing Workflows for Biologists: A Roadmap

    OpenAIRE

    Shade, Ashley; Teal, Tracy K.

    2015-01-01

    Extremely large datasets have become routine in biology. However, performing a computational analysis of a large dataset can be overwhelming, especially for novices. Here, we present a step-by-step guide to computing workflows with the biologist end-user in mind. Starting from a foundation of sound data management practices, we make specific recommendations on how to approach and perform computational analyses of large datasets, with a view to enabling sound, reproducible biological research.

  18. Proton/Phosphate Stoichiometry in Uptake of Inorganic Phosphate by Cultured Cells of Catharanthus roseus (L.) G. Don

    Science.gov (United States)

    Sakano, Katsuhiro

    1990-01-01

    Upon absorption of phosphate, cultured cells of Catharanthus roseus (L.) G. Don caused a rapid alkalinization of the medium in which they were suspended. The alkalinization continued until the added phosphate was completely exhausted from the medium, at which time the pH of the medium started to drop sharply toward the original pH value. Phosphate exposure caused the pH of the medium to increase from pH 3.5 to values as high as 5.8, while the rate of phosphate uptake was constant throughout (10-17 micromoles per hour per gram fresh weight). This indicates that no apparent pH optimum exists for the phosphate uptake by the cultured cells. The amount of protons cotransported with phosphate was calculated from the observed pH change up to the maximum alkalinization and the titration curve of the cell suspension. Proton/phosphate transport stoichiometry ranged from less than unity to 4 according to the amount of phosphate applied. At low phosphate doses, the stoichiometries were close to 4, while at high phosphate doses, smaller stoichiometries were observed. This suggests that, at high phosphate doses, activation of the proton pump is induced by the longer lasting proton influx acidifying the cytoplasm. The increased H+ efflux due to the proton pump could partially compensate protons taken up via the proton-phosphate cotransport system. Thus, the H+/H2PO4− stoichiometry of the cotransport is most likely to be 4. PMID:16667491

  19. Expanding the Biologist's Toolkit with CRISPR-Cas9.

    Science.gov (United States)

    Sternberg, Samuel H; Doudna, Jennifer A

    2015-05-21

    Few discoveries transform a discipline overnight, but biologists today can manipulate cells in ways never possible before, thanks to a peculiar form of prokaryotic adaptive immunity mediated by clustered regularly interspaced short palindromic repeats (CRISPR). From elegant studies that deciphered how these immune systems function in bacteria, researchers quickly uncovered the technological potential of Cas9, an RNA-guided DNA cleaving enzyme, for genome engineering. Here we highlight the recent explosion in visionary applications of CRISPR-Cas9 that promises to usher in a new era of biological understanding and control.

  20. Entropy for biologists an introduction to thermodynamics

    CERN Document Server

    Morowitz, Harold J

    2013-01-01

    Entropy for Biologists: An Introduction to Thermodynamics is an introductory book for people in the life sciences who wish to master the concepts of thermal physics without being forced to a degree and rate of symbol manipulation which is foreign to their patterns of thought. The book opens with a chapter on temperature, followed by separate chapters that discuss the concepts of energy, kinetic theory, total energy, the second law of thermodynamics, entropy, and probability and information theory. Subsequent chapters deal with statistical mechanics and its relation to thermodynamics, free-ener

  1. Which statistics should tropical biologists learn?

    Science.gov (United States)

    Loaiza Velásquez, Natalia; González Lutz, María Isabel; Monge-Nájera, Julián

    2011-09-01

    Tropical biologists study the richest and most endangered biodiversity in the planet, and in these times of climate change and mega-extinctions, the need for efficient, good quality research is more pressing than in the past. However, the statistical component in research published by tropical authors sometimes suffers from poor quality in data collection; mediocre or bad experimental design and a rigid and outdated view of data analysis. To suggest improvements in their statistical education, we listed all the statistical tests and other quantitative analyses used in two leading tropical journals, the Revista de Biología Tropical and Biotropica, during a year. The 12 most frequent tests in the articles were: Analysis of Variance (ANOVA), Chi-Square Test, Student's T Test, Linear Regression, Pearson's Correlation Coefficient, Mann-Whitney U Test, Kruskal-Wallis Test, Shannon's Diversity Index, Tukey's Test, Cluster Analysis, Spearman's Rank Correlation Test and Principal Component Analysis. We conclude that statistical education for tropical biologists must abandon the old syllabus based on the mathematical side of statistics and concentrate on the correct selection of these and other procedures and tests, on their biological interpretation and on the use of reliable and friendly freeware. We think that their time will be better spent understanding and protecting tropical ecosystems than trying to learn the mathematical foundations of statistics: in most cases, a well designed one-semester course should be enough for their basic requirements.

  2. Stem cell therapy for white matter disorders: don't forget the microenvironment!

    Science.gov (United States)

    Dooves, Stephanie; van der Knaap, Marjo S; Heine, Vivi M

    2016-07-01

    White matter disorders (WMDs) are a major source of handicap at all ages. They often lead to progressive neurological dysfunction and early death. Although causes are highly diverse, WMDs share the property that glia (astrocytes and oligodendrocytes) are among the cells primarily affected, and that myelin is either not formed or lost. Many WMDs might benefit from cell replacement therapies. Successful preclinical studies in rodent models have already led to the first clinical trials in humans using glial or oligodendrocyte progenitor cells aiming at (re)myelination. However, myelin is usually not the only affected structure. Neurons, microglia, and astrocytes are often also affected and are all important partners in creating the right conditions for proper white matter repair. Composition of the extracellular environment is another factor to be considered. Cell transplantation therapies might therefore require inclusion of non-oligodendroglial cell types and target more than only myelin repair. WMD patients would likely benefit from multimodal therapy approaches involving stem cell transplantation and microenvironment-targeting strategies to alter the local environment to a more favorable state for cell replacement. Furthermore most proof-of-concept studies have been performed with human cells in rodent disease models. Since human glial cells show a larger regenerative capacity than their mouse counterparts in the host mouse brain, microenvironmental factors affecting white matter recovery might be overlooked in rodent studies. We would like to stress that cell replacement therapy is a highly promising therapeutic option for WMDs, but a receptive microenvironment is crucial. PMID:27000179

  3. Cell Phones in the Classroom: Don't Put Them Away Just Yet!

    Science.gov (United States)

    Webb, Carolyn Lorraine

    2013-01-01

    As the number of students with cell phones has steadily increased over the past decade, these technological advances have caused trepidation among educators over behavioral issues, from off-task activities to cheating (Prensky, 2005). On the other hand, mobile learning, as well as some tools to use with cell phones, are easy to set up, easy to…

  4. State-transition diagrams for biologists.

    Science.gov (United States)

    Bersini, Hugues; Klatzmann, David; Six, Adrien; Thomas-Vaslin, Véronique

    2012-01-01

    It is clearly in the tradition of biologists to conceptualize the dynamical evolution of biological systems in terms of state-transitions of biological objects. This paper is mainly concerned with (but obviously not limited too) the immunological branch of biology and shows how the adoption of UML (Unified Modeling Language) state-transition diagrams can ease the modeling, the understanding, the coding, the manipulation or the documentation of population-based immune software model generally defined as a set of ordinary differential equations (ODE), describing the evolution in time of populations of various biological objects. Moreover, that same UML adoption naturally entails a far from negligible representational economy since one graphical item of the diagram might have to be repeated in various places of the mathematical model. First, the main graphical elements of the UML state-transition diagram and how they can be mapped onto a corresponding ODE mathematical model are presented. Then, two already published immune models of thymocyte behavior and time evolution in the thymus, the first one originally conceived as an ODE population-based model whereas the second one as an agent-based one, are refactored and expressed in a state-transition form so as to make them much easier to understand and their respective code easier to access, to modify and run. As an illustrative proof, for any immunologist, it should be possible to understand faithfully enough what the two software models are supposed to reproduce and how they execute with no need to plunge into the Java or Fortran lines.

  5. The Don Bosco Story.

    Science.gov (United States)

    Galza, Ma. Lourdes LL.

    1985-01-01

    Describes the work of the Don Bosco Educational Association of the Philippines, which operates trade schools for boys. The article focuses on the Don Bosco College's engineering technology and general engineering science programs and the development of Industrial Technician Centers. Other programs operated by the Salesians are also described. (CT)

  6. Lack of Control in Inorganic Phosphate Uptake by Catharanthus roseus (L.) G. Don Cells (Cytoplasmic Inorganic Phosphate Homeostasis Depends on the Tonoplast Inorganic Phosphate Transport System?).

    Science.gov (United States)

    Sakano, K.; Yazaki, Y.; Okihara, K.; Mimura, T.; Kiyota, S.

    1995-01-01

    Inorganic phosphate (Pi) uptake by Catharanthus roseus (L.) G. Don cells was studied in relation to its apparent uncontrolled uptake using 31P-nuclear magnetic resonance spectroscopy. Kinetics of Pi uptake by the cells indicated that apparent Km and Vm were about 7 [mu]M and 20 [mu]mol g-1 fresh weight h-1, respectively. Pi uptake in Murashige-Skoog medium under different Pi concentrations and different initial cell densities followed basically the same kinetics. When supplied with abundant Pi, cells absorbed Pi at a constant rate (Vm) for the first hours and accumulated it in the vacuole. As the endogenous pool expanded, the rate of Pi uptake gradually decreased to nil. Maximum Pi accumulation was 100 to 120 [mu]mol g-1 fresh weight if cell swelling during Pi uptake (about 2-fold in cell volume) was not considered. Results indicated that (a) the rate of Pi uptake by Catharanthus cells was independent of initial cell density and was constant over a wide range of Pi concentrations (2 mM to about 10 [mu]M) unless the cells were preloaded with excess Pi, and (b) there was no apparent feedback control over the Pi uptake process in the plasma membrane to avoid Pi toxicity. The importance of the tonoplast Pi transport system in cytoplasmic Pi homeostasis is discussed. PMID:12228474

  7. Answering evolutionary questions: A guide for mechanistic biologists.

    Science.gov (United States)

    Masel, Joanna; Promislow, Daniel E L

    2016-07-01

    The questions and methods of molecular biology and evolutionary biology are clearly distinct, yet a unified approach can lead to deep insights. Unfortunately, attempts to unify these approaches are fraught with pitfalls. In this informal series of questions and answers, we offer the mechanistically oriented biologist a set of steps to come up with evolutionarily reasonable and meaningful hypotheses. We emphasize the critical power and importance of carefully constructed null hypotheses, and we illustrate our ideas with examples representing a range of topics, from the biology of aging, to protein structure, to speciation, and more. We also stress the importance of mathematics as the lingua franca for biologists of all stripes, and encourage mechanistic biologists to seek out quantitative collaborators to build explicit mathematical models, making their assumptions explicit, and their logic clear and testable. Biologists in all realms of inquiry stand to gain from strong bridges between our disciplines. PMID:27151396

  8. Attributes of Biologists in Two-Year Colleges

    Science.gov (United States)

    Creager, Joan G.; Ehrle, Elwood B.

    1971-01-01

    Presents results of a questionnaire survey of sex, age, qualifications, work experience, teaching load, research and publication activities, professional society membership, and salaries of biologists employed in junior colleges in the United States. (AL)

  9. Flavonoids isolated from Lespedeza cuneata G. Don and their inhibitory effects on nitric oxide production in lipopolysaccharide-stimulated BV - 2 microglia cells

    Directory of Open Access Journals (Sweden)

    Guijae Yoo

    2015-01-01

    Full Text Available Background: Lespedeza cuneata (Dum. Cours. G. Don, a perennial legume native to Eastern Asia, has been used therapeutically in traditional Asian medicine to protect the function of liver, kidneys and lungs. However, its effect on inflammatory nitric oxide (NO production and the active constituents have not yet been explored. Objective: In this study, we investigated the phytochemical constituents of L. cuneata and evaluated their effect on NO production using lipopolysaccharide (LPS stimulated BV2 cells. Materials and Methods: The 80% methanol extract of the aerial part of L. cuneata were used for the isolation of flavonoids. The isolated compounds were elucidated by various spectroscopic methods including nuclear magnetic resonance and mass spectrometry spectrometry. To evaluate the effect on inflammatory NO production, LPS stimulated murine microglia BV 2 cells were used as a screening system. Results: Nine flavonoids were isolated from the aerial parts of L. cuneata. Among the isolated flavonoids, compounds 4, 5, 7 and 9 are reported from the genus Lespedeza for the first time. Moreover, compounds 1 and 6 showed significant inhibitory effects on NO production in LPS stimulated BV2 cells without cell toxicity. Conclusion: In this study, nine flavonoids were isolated from L. cuneata. Among the compounds, only 1 and 6, which have free hydroxyl groups at both C3 and C7 showed significant inhibitory activity on NO production in LPS stimulated BV2 cells. These results suggested L. cuneata and its flavonoid constituents as possible candidate for the treatment of various inflammatory diseases.

  10. Dr Jacob van der Land, marine biologist extraordinary

    NARCIS (Netherlands)

    Bruggen, van A.C.

    2001-01-01

    This contribution is an attempt to sketch the life and works of Dr Jacob van der Land, curator of worms and chief marine biologist of the National Museum of Natural History, on the occasion of his official retirement. Born in 1935, Jacob van der Land read biology at Leiden University (1958-1964), wh

  11. Don Eddy; "Jewelry."

    Science.gov (United States)

    Schaefer, Claire

    1989-01-01

    Presents a lesson that introduces students in grades K-three to sources of design inspiration in contemporary urban settings. Using Don Eddy's painting of a jewelry store window display, asks students to describe and analyze the interplay of shape, pattern, and color. Suggests studio activities, including an activity in which students build a…

  12. Don Quijote y Hamlet

    Directory of Open Access Journals (Sweden)

    Lúdovik B. Osterc

    2002-12-01

    Hamlet está loco, es un alucinado que duda de sus propios sentidos. Para él existe sólo un lado negro de la existencia, su único objeto es llegar más pronto al descubrimiento del crimen. Es egocentrico, ne cree en nada, eternamente mira en su interior, se alimenta en su desprecio de sí mismo y su sufrimiento es mucho más doloroso.que aquel de Don Quijote. Don Quijote, al contrario, cree en el hombre y su futuro. Su ideal es implantar por la fuerza, la verdad, el bien y la justicia en la tierra. Para servir a su ideal, está dispuesto a sufrir todas las posibles privaciones. Representa la fe en la verdad. Es ridículo y quizás el más cómico tipo disenado jamás por un literato. Hamlet es el hijo del rey muerte, mientras que Don Quijote está pobre. Se hace los enemigos imaginarios y lucha contra ellos, mientras que Hamlet no cree en las ilusiones (siempre duda, y tampoco no combatiría si supiera que son sinónimos de opresores. Continua con la examinación de la actitud del pueblo o sus representantes hacia Don Quijote y Hamlet y viceversa. Polonia es el representante de las multitudes populares ante Hamlet, Sancho lo es ante su senor. El primero es de sano pensamiento y al mismo tiempo un viejo charalatán. Para él Hamlet no es tanto un loco como un nino y le parece inútil para la sociedad. Sancho Pansa está persuadido de que su senor está loco, pero le está leal hasta la muerte. Don Quijote simpatiza profundamente con el pueblo, pero la única persona con la que se entiende verdaderamente es Sancho Pansa (uno no puede vivir sin otro. La actitud de Hamlet es completamente diferente, él desprecia las multitudes populares, porque también desprecia a sí mismo. social: el tedio, el hastío, el descontento de la vida, desorientación espiritual y la eclipse de la fe, miéntras que Don Quijote encarna el mundo libre, bien estar y la felicidad de los seres humanos. Por eso y porque es un ideal el más humano, generoso, noble y elevado, el autor del

  13. The Metabolic Fate of Deoxynivalenol and Its Acetylated Derivatives in a Wheat Suspension Culture: Identification and Detection of DON-15-O-Glucoside, 15-Acetyl-DON-3-O-Glucoside and 15-Acetyl-DON-3-Sulfate

    Directory of Open Access Journals (Sweden)

    Clemens Schmeitzl

    2015-08-01

    Full Text Available Deoxynivalenol (DON is a protein synthesis inhibitor produced by the Fusarium species, which frequently contaminates grains used for human or animal consumption. We treated a wheat suspension culture with DON or one of its acetylated derivatives, 3-acetyl-DON (3-ADON, 15-acetyl-DON (15-ADON and 3,15-diacetyl-DON (3,15-diADON, and monitored the metabolization over a course of 96 h. Supernatant and cell extract samples were analyzed using a tailored LC-MS/MS method for the quantification of DON metabolites. We report the formation of tentatively identified DON-15-O-β-D-glucoside (D15G and of 15-acetyl-DON-3-sulfate (15-ADON3S as novel deoxynivalenol metabolites in wheat. Furthermore, we found that the recently identified 15-acetyl-DON-3-O-β-D-glucoside (15-ADON3G is the major metabolite produced after 15-ADON challenge. 3-ADON treatment led to a higher intracellular content of toxic metabolites after six hours compared to all other treatments. 3-ADON was exclusively metabolized into DON before phase II reactions occurred. In contrast, we found that 15-ADON was directly converted into 15-ADON3G and 15-ADON3S in addition to metabolization into deoxynivalenol-3-O-β-D-glucoside (D3G. This study highlights significant differences in the metabolization of DON and its acetylated derivatives.

  14. Reinventing College Physics for Biologists: Explicating an epistemological curriculum

    OpenAIRE

    Edward F. Redish; Hammer, David

    2008-01-01

    The University of Maryland Physics Education Research Group (UMd-PERG) carried out a five-year research project to rethink, observe, and reform introductory algebra-based (college) physics. This class is one of the Maryland Physics Department's large service courses, serving primarily life-science majors. After consultation with biologists, we re-focused the class on helping the students learn to think scientifically -- to build coherence, think in terms of mechanism, and to follow the implic...

  15. Production Pattern of Ajmalicine in Catharanthus roseus (L.) G. Don. Cell Aggregates Culture in the Airlift Bioreactor

    OpenAIRE

    RIZKITA RACHMI ESYANTI; AIDA MUSPIAH

    2006-01-01

    A research has been conducted to optimize the rate of aeration and initial weight of cell aggregates in the production of ajmalicine in Catharanthus roseus cell culture in airlift bioreactor. Catharanthus roseus culture were grown in Zenk medium with the addition of 2.50 x 10-6 M naphthalene acetic acid (NAA) and 10-5 M benzyl amino purine (BAP). Cell aggregates were sub-cultured two times before transferring 20 and 30 g/fw of cell aggregates into bioreactor, respectively, and aerated with th...

  16. "Owl's eye" cells in a cervical smear of a transplant recipient: don't forget to inform the referring physician.

    NARCIS (Netherlands)

    Oei, A.L.M.; Salet-van de Pol, M.R.J. van de; Borst, S.M.; Berg, A.P. van den; Grefte, J.M.M.

    2007-01-01

    Cytomegalovirus (CMV) infected cells in cervical smears are a rare finding but may have severe consequences. We describe the presence of characteristic "owl eye" cells in a conventional cervical smear. Medical history revealed a liver transplantation from a CMV seropositive donor 1 yr earlier. The p

  17. 'Owl's eye' cells in a cervical smear of a transplant recipient : Don't forget to inform the referring physician

    NARCIS (Netherlands)

    Oei, A. L. M.; Salet-van de Pol, M. R. J.; Borst, S. M.; van den Berg, A. P.; Grefte, J. M. M.

    2007-01-01

    Cytomegalovirus (CMV) infected cells in cervical smears are a rare finding but may have severe consequences. We describe the presence of characteristic 'owl eye' cells in a conventional cervical smear. Medical history revealed a liver transplantation from a CMV seropositive donor 1 yr earlier. The p

  18. Production Pattern of Ajmalicine in Catharanthus roseus (L. G. Don. Cell Aggregates Culture in the Airlift Bioreactor

    Directory of Open Access Journals (Sweden)

    RIZKITA RACHMI ESYANTI

    2006-12-01

    Full Text Available A research has been conducted to optimize the rate of aeration and initial weight of cell aggregates in the production of ajmalicine in Catharanthus roseus cell culture in airlift bioreactor. Catharanthus roseus culture were grown in Zenk medium with the addition of 2.50 x 10-6 M naphthalene acetic acid (NAA and 10-5 M benzyl amino purine (BAP. Cell aggregates were sub-cultured two times before transferring 20 and 30 g/fw of cell aggregates into bioreactor, respectively, and aerated with the rate of 0.25 l min-1 and 0.34 l min-1, respectively. The pattern of ajmalicine production in bioreactor were observed in every three days within 24 days. Qualitative and quantitative analysis were conducted using HPLC connected to Cromatopac CL-7A Plus. The results showed that the cell aggregates and medium contain ajmalicine. The highest concentration was obtained in combination of 30 g/fw and 0.34 l min-1 aeration compare to 20 g/fw - 0.25 l min-1, 20 g/fw - 0.34 l min-1, as well as 30 g/fw – 0.25 l min-1. The highest ajmalicine content in cell aggregates was obtained on the 12 days (79.23 µg g-1 whilst in medium was obtained in the 18th days (981.15 µg l-1.

  19. Preeminent Biologist Joins CAS to Lead a New Research Center in Shanghai

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Dr. ZHU Jiankang, an internationally renowned plant cell biologist and member of the US National Academy of Sciences, will work as Director of the Shanghai Center for Plant Stress Biology, which was officially inaugurated on April 28, 2012 in suburban Shanghai. Before he joined CAS through a key talent recruitment program sponsored by the Chinese government called the "Thousand Talents Program", Dr. ZHU had worked for many universities in the world and carried out leading innovative research on genetic and epigenetic mechanisms of plant responses to adverse environments such as salinity, drought and low temperature.

  20. Mimesis in Don Quixote

    Directory of Open Access Journals (Sweden)

    Giselle von der Walde

    2006-04-01

    Full Text Available Resumen:Frente a argumentos tomados de las poéticas neoaristotélicas que esgrime el canónigo para condenar los libros de caballerías, don Quijote pretende mostrar con su propio ejemplo, que ese tipo de lecturas no llevan a la locura ni al abandono de sí mismo, sino que, por el contrario, sacan lo mejor de la naturaleza de un individuo y lo hacen mejor persona. Este es el único tipo de imitación que Platón acepta en la República, pues más que un remedo o una suplantación, implica emulación. Este escrito se propone mirar, a partir del diálogo con el canónigo de Toledo (I, 49-50, cómo en sus conductas miméticas desde el comienzo de la obra, don Quijote entiende la imitación como emulación; en consecuencia se intenta demostrar que el efecto de la literatura en el caballero manchego parece ser el tipo de mimesis que Platón acepta en el libro III de la República.Abstract:Don Quijote demonstrates with the example of his own life the error made by the Canon of Toledo when the latter uses the arguments of the neo-Aristotelian poets to condemn the novels of chivalric romance. Quijote tries to show that reading the chivalric romances leads to neither madness nor the abandonment of the self, but, on the contrary, brings out the best in a person’s nature, and makes one a better person. This is the only type of imitation that Plato accepts in the Republic, because, unlike the mere copying of a person, or the substitution of one person for another, it implies a type of emulation. Starting with the dialogue of the Canon of Toledo (I, 49-50, this paper shows how, in his mimetic behavior, Don Quijote understands imitation as emulation. As a result, the paper shows that the effect of literature on the gentleman from La Mancha is the type of mimesis of which Plato approves in book III of the Republic.

  1. The UCSC Genome Browser: What Every Molecular Biologist Should Know

    Science.gov (United States)

    Mangan, Mary E.; Williams, Jennifer M.; Kuhn, Robert M.; Lathe, Warren C.

    2016-01-01

    Electronic data resources can enable molecular biologists to query and display many useful features that make benchwork more efficient and drive new discoveries. The UCSC Genome Browser provides a wealth of data and tools that advance one’s understanding of genomic context for many species, enable detailed understanding of data, and provide the ability to interrogate regions of interest. Researchers can also supplement the standard display with their own data to query and share with others. Effective use of these resources has become crucial to biological research today, and this unit describes some practical applications of the UCSC Genome Browser. PMID:19816931

  2. Improving interactions between animal rights groups and conservation biologists.

    Science.gov (United States)

    Perry, Dan; Perry, Gad

    2008-02-01

    Invasive species are often considered to be a major threat to biodiversity, leading conservation biologists to often recommend their complete eradication. Animal rights groups typically categorically oppose killing animals, and their opposition has brought eradication attempts of gray squirrels in northern Italy (Europe) and mute swans in Vermont to a halt. As a result native red squirrels may disappear from Europe and ecosystem-wide impacts are expected to be caused by the swan. In contrast, cooperation between managers and animal rights groups has resulted in a successful control program for feral pigs in Fort Worth, Texas (U.S.A.). The philosophical differences between animal rights and conservation biologists' views make cooperation seem unlikely, yet documented cases of cooperation have been beneficial for both groups. We recommend that managers dealing with invasive species should consult with social scientists and ethicists to gain a better understanding of the implications of some of their policy decisions. In addition, we recommend that animal rights groups do more to support alternatives to lethal control, which are often excluded by economic limitations. Prevention of arrival of invasive species via application of the precautionary principle may be an especially productive avenue for such collaboration because it fits the goals and values of both groups.

  3. Don't panic

    Science.gov (United States)

    Harris, Margaret

    2009-06-01

    "Don't panic" is a simple piece of advice, one that usually applies equally well to job-hunting, avoiding pandemic flu or, like the hapless Arthur Dent in the Hitchhiker's Guide to the Galaxy stories, wandering the universe in search of a decent cup of tea. Yet some statistics emerging from the current job market are undeniably alarming. Postings on the recruitment website Milkround.com, which advertises jobs and internships for recent graduates, are down by about 20% compared with this time last year, while a survey earlier this year by High Fliers, a Londonbased research firm, found that top UK employers plan to recruit 17% fewer graduates this year than in 2008. In the same study, half of the 1017 final-year students surveyed believed they would have to take "any graduate job" they were offered, regardless of their interest in the company, and a whopping 91% thought competition for vacancies would be tougher than last year.

  4. Reinventing College Physics for Biologists: Explicating an epistemological curriculum

    CERN Document Server

    Redish, Edward F

    2008-01-01

    The University of Maryland Physics Education Research Group (UMd-PERG) carried out a five-year research project to rethink, observe, and reform introductory algebra-based (college) physics. This class is one of the Maryland Physics Department's large service courses, serving primarily life-science majors. After consultation with biologists, we re-focused the class on helping the students learn to think scientifically -- to build coherence, think in terms of mechanism, and to follow the implications of assumptions. We designed the course to tap into students' productive conceptual and epistemological resources, based on a theoretical framework from research on learning. The reformed class retains its traditional structure in terms of time and instructional personnel, but we modified existing best-practices curricular materials, including Peer Instruction, Interactive Lecture Demonstrations, and Tutorials. We provided class-controlled spaces for student collaboration, which allowed us to observe and record stud...

  5. Flavonoids isolated from Lespedeza cuneata G. Don and their inhibitory effects on nitric oxide production in lipopolysaccharide-stimulated BV-2 microglia cells

    OpenAIRE

    Guijae Yoo; Seon Ju Park; Taek Hwan Lee; Heejung Yang; Yoon-su Baek; Nanyoung Kim; Yoon Jae Kim; Seung Hyun Kim

    2015-01-01

    Background: Lespedeza cuneata (Dum. Cours.) G. Don, a perennial legume native to Eastern Asia, has been used therapeutically in traditional Asian medicine to protect the function of liver, kidneys and lungs. However, its effect on inflammatory nitric oxide (NO) production and the active constituents have not yet been explored. Objective: In this study, we investigated the phytochemical constituents of L. cuneata and evaluated their effect on NO production using lipopolysaccharide (LPS) stimul...

  6. In vitro effect of deoxynivalenol (DON) mycotoxin on porcine reproductive and respiratory syndrome virus replication.

    Science.gov (United States)

    Savard, Christian; Pinilla, Vicente; Provost, Chantale; Segura, Mariela; Gagnon, Carl A; Chorfi, Younes

    2014-03-01

    Deoxynivalenol (DON) is a mycotoxin produced by Fusarium spp. Among monogastric farm animals, swine are the most susceptible to DON as it markedly reduces feed intake and decreases weight gain. DON has also been shown to increase susceptibility to viral infections; therefore the objective of this study was to investigate in vitro impact of DON on porcine reproductive and respiratory syndrome virus (PRRSV). Permissive cells were infected or not with PRRSV and were treated with increasing concentrations of DON. Cell survival and mortality were evaluated by determining the number of viable cells with a tetrazolium compound and by measuring lactate dehydrogenase (LDH) release, respectively. Virus titration and antiviral cytokines mRNA expression were evaluated by quantitative PCR. DON significantly affected the survival of noninfected cells in a dose dependent manner. However, DON concentrations between 140 and 280 significantly increased the survival of cells infected with PRRSV. These concentrations significantly decreased PRRSV replication by inducing a pro-inflammatory cytokines environment and an early activation of apoptosis, which in turn seem to interrupt viral replication. For the first time, this study showed that DON had significant effects on the survival of PRRSV infected cells and on virus replication, in a dose dependent manner.

  7. "Tihhi Don" - za i protiv

    Index Scriptorium Estoniae

    2006-01-01

    Võrdluses on Mihhail Sholohhovi raamatu "Vaikne Don" kaks ekraniseeringut : Sergei Gerassimovi (1975) ja Sergei Bondartshuki (1991) oma. Võrreldakse peaosatäitjaid, meenutatakse huvitavaid fakte filmivõtetelt. Katkend Elina Bõstritskaja mälestusteraamatust

  8. Pregnant? Don't Smoke!

    Science.gov (United States)

    ... What's this? Submit Button Past Emails CDC Features Pregnant? Don't Smoke! Language: English Español (Spanish) Recommend ... problems. The Good News Quitting smoking before getting pregnant is best. But for women who are already ...

  9. Odgajajmo s don Boscovim srcem

    OpenAIRE

    Chávez Villanueva, Pascual

    2008-01-01

    Don Bosco svojim životnim primjerom kao odgajatelj pokazuje kako je uspješan odgoj ujedno i kvalitetan odgoj. On je svugdje i na svakom mjestu bio odgajatelj. Za dobar odgoj potrebni su: dobar odgajatelj, dobro odgojno ozračje, dobar odgojni program i dobro odgojno iskustvo. Don Boscovo mjerilo u odgoju je razvoj pozitivnog poticaja odnosno pozitivne želje koji postoje u svakom mladom čovjeku. To uključuje i povezanost s kulturnom baštinom i ponudu iskustva vjere. Odgajanika se uključuje u dr...

  10. Kulturel liminalitet i Don Quixote

    DEFF Research Database (Denmark)

    Fastrup, Anne

    2007-01-01

    Med udgangspunkt i og i kontrast til nogle af hovedlinierne i den nyere litteratur om Don Quixote søger artiklen tilbage til de mere specifikke og lokale historiske forhold, som er romanens baggrund, bl.a. til Cervantes' »algierske erfaring« med tyrkisk fangenskab. Artiklen forsøger at kombinere ...

  11. Rejoice in the hubris: useful things biologists could do for physicists

    Science.gov (United States)

    Austin, Robert H.

    2014-10-01

    Political correctness urges us to state how wonderful it is to work with biologists and how, just as the lion will someday lie down with the lamb, so will interdisciplinary work, where biologists and physicists are mixed together in light, airy buildings designed to force socialization, give rise to wonderful new science. But it has been said that the only drive in human nature stronger than the sex drive is the drive to censor and suppress, and so I claim that it is OK for physicists and biologists to maintain a wary distance from each other, so that neither one censors or suppresses the wild ideas of the other.

  12. Don Ross, Philosophy of Economics

    OpenAIRE

    Claveau, François

    2016-01-01

    A possible misunderstanding must be addressed at the outset. In this book, Don Ross offers his philosophy of economics, not a survey of an area of contemporary philosophy that is conventionally referred to as philosophy of economics. Ross is explicit about that (Author’s preface, x). In consequence, the book is an outlier as a title in the series Palgrave Philosophy Today, a series “meant to help all philosophers, established and aspiring, to understand, appreciate and engage with the intrica...

  13. Regulation of IGF-I production and proliferation of human leiomyomal smooth muscle cells by Scutellaria barbata D. Don in vitro: isolation of flavonoids of apigenin and luteolin as acting compounds

    International Nuclear Information System (INIS)

    Scutellaria barbata D. Don (Lamiaceae) (SB) is a perennial herb, which is natively distributed throughout Korea and southern China. This herb is known in traditional Chinese Medicine as Ban-Zhi-Lian and traditional Korean medicine as Banjiryun, respectively. SB has been used as an anti-inflammatory and antitumor agent. We aimed to determine the expression of growth factor molecules for growth inhibition after treatment of SB in two different human myometrial smooth muscle cell (SMC)s and leiomyomal SMCs. Water-soluble ingredients of SB, myometrial SMCs, and the leiomyomal cell lines were used in vitro. SB significantly reduced cell numbers in culture and arrested cell proliferation, and also induced apoptosis, indicating that the presence of an intact apoptotic pathway was demonstrated in these cells by SB. Uterine leiomyoma is the most common benign smooth muscle cell tumor of the myometrium. The expression of insulin-like growth factor-I (IGF-I) was measured at the mRNA and protein level in myometrium and leiomyomal cells with and without treatment with a water extract of SB for 3 days. IGF-I mRNA expression was significantly higher in leiomyomal cells than in myometrium cells. The IGF-I protein was more abundant in leiomyomal cells than in myometrium. When SB was treated to the cells, the IGF-I protein concentrations in myometrial and leiomyomal cells from the SB-treated cells were similar. The results indicated that IGF-I expression is probably associated with a proliferation of leiomyomal cells than myometrium. However, SB down-regulated the IGF-I expression where IGF-I contributes to the selective growth of the leiomyoma. Therefore, growth modulation of LMs by SB occurs via mechanisms dependent of apoptosis. The raw materials were extracted and subjected to functional isolation for the active molecules in the present assay systems. The five flavonoids were isolated and the chemical structures of resveratrol, baicalin, berberine, apigenin, and luteolin were

  14. Biologists use mathematics to advance our understanding of health, disease

    OpenAIRE

    Trulove, Susan

    2010-01-01

    Math-based computer models are a powerful tool for discovering the details of complex living systems. John Tyson, professor of biology at Virginia Tech, is creating such models to discover how cells process information and make decisions.

  15. Stewart, Don oral history interview

    OpenAIRE

    Interviewee: Stewart, Don; Interviewer: Manlove, Courtney; Principal Investigator: Hall, Peter V

    2014-01-01

    Don Stewart was born at St Mary’s Hospital in New Westminster in 1944, and had six siblings. He was the son of a mill worker at MacMillan Bloedel, who was also a caretaker at Spring Ranch located in the Connaught Heights area of New Westminster. It was called Spring Ranch because it had its own “artesian well” or spring that generated two small pools that Stewart and his friends would swim in during the summer. The family lived on the ranch between 1944 and 1952, and Stewart’s descriptions of...

  16. Don DeLillos Bilder

    Directory of Open Access Journals (Sweden)

    Diesner, Fabian

    2005-01-01

    Full Text Available The American author Don DeLillo is a master of creating powerful images, which want to be discovered, read and interpreted. To have a closer look at his novel Mao II from the Visual Studies' point of view reveals the complexity of literary images and the diverse references that are evoked. DeLillo plays with the reader's memory of perception, asks fundamental questions about our society ruled by mass media and simply delights with a picturesque style of narration. The central aspects in his novel are photography in general, the visual arts especially by Andy Warhol and the visual representation of mass media, particularly television.

  17. [Don Quijote, a lucid mad].

    Science.gov (United States)

    Alonso Fernández, Francisco

    2004-01-01

    The case of a 50-year-old hidalgo who believed to be transformed into a knight-errant named Don Quijote is a megalomaniac systematized delusion of transformation of the self, a delusion of metamorphosis in reference to the patient's own identity. The outward projection of this syndrome produces some delusional misidentifications of others, things and animals and include elements of a persecutory delusion which increase the grandiosity of the self. At the same time the hidalgo was maniac with a pathway of bipolar disorder. The phenomenon of donquijotismo is described as the defence of wasted causes and Sancho Panza as an illiterate Sócrates. PMID:15997586

  18. [Don Quijote, a lucid mad].

    Science.gov (United States)

    Alonso Fernández, Francisco

    2004-01-01

    The case of a 50-year-old hidalgo who believed to be transformed into a knight-errant named Don Quijote is a megalomaniac systematized delusion of transformation of the self, a delusion of metamorphosis in reference to the patient's own identity. The outward projection of this syndrome produces some delusional misidentifications of others, things and animals and include elements of a persecutory delusion which increase the grandiosity of the self. At the same time the hidalgo was maniac with a pathway of bipolar disorder. The phenomenon of donquijotismo is described as the defence of wasted causes and Sancho Panza as an illiterate Sócrates.

  19. Putting computational modeling at the fingertips of bench biologists

    DEFF Research Database (Denmark)

    Sonnenschein, Nikolaus; Herrgard, Markus

    Communication is key in successful collaborations between theoretical and experimental life scientists. In our line of work we integrate physiological and systems-level data of cell factories with constraint-based Modeling approaches to predict suitable targets for metabolic engineering. The most...... important step in this process is the discourse and prioritization of strategies with the people that actually implement them. Having worked with a particular host organism for many years, experimentalists can often discard strategies based on previous experience or feasibility. Counterintuitive solutions...

  20. The riddle of ``life,'' a biologist's critical view

    Science.gov (United States)

    Penzlin, Heinz

    2009-01-01

    To approach the question of what life is, we first have to state that life exists exclusively as the “being-alive” of discrete spatio-temporal entities. The simplest “unit” that can legitimately be considered to be alive is an intact prokaryotic cell as a whole. In this review, I discuss critically various aspects of the nature and singularity of living beings from the biologist’s point of view. In spite of the enormous richness of forms and performances in the biotic realm, there is a considerable uniformity in the chemical “machinery of life,” which powers all organisms. Life represents a dynamic state; it is performance of a system of singular kind: “life-as-action” approach. All “life-as-things” hypotheses are wrong from the beginning. Life is conditioned by certain substances but not defined by them. Living systems are endowed with a power to maintain their inherent functional order (organization) permanently against disruptive influences. The term organization inherently involves the aspect of functionality, the teleonomic, purposeful cooperation of structural and functional elements. Structures in turn require information for their specification, and information presupposes a source. This source is constituted in living systems by the nucleic acids. Organisms are unique in having a capacity to use, maintain, and replicate internal information, which yields the basis for their specific organization in its perpetuation. The existence of a genome is a necessary condition for life and one of the absolute differences between living and non-living matter. Organization includes both what makes life possible and what is determined by it. It is not something “implanted” into the living beings but has its origin and capacity for maintenance within the system itself. It is the essence of life. The property of being alive we can consider as an emergent property of cells that corresponds to a certain level of self-maintained complex order or

  1. ``Don't Lecture Me''

    Science.gov (United States)

    Blanton, Patricia

    2011-11-01

    Often I will listen to public radio on long drives when I am alone. Recently I happened to catch a program called ``Don't Lecture Me'' and it really caught my attention for several reasons. First, the speakers were all notable leaders in Physics Education Research such as Joe Redish, David Hestenes, and Eric Mazur. (See this month's WebSights column.) These folks are among many who have devoted their energies to understanding how students learn physics and how teachers can design classroom instruction and interactions to best meet the needs of learners. Second, on this particular trip, I had just observed a teacher whose class was very teacher-centered as the teacher lectured most of the class period. As we discussed this later, she expressed concern that she had to cover the material and didn't feel that she could do it without lecturing.

  2. "Don't Lecture Me"

    Science.gov (United States)

    2011-11-01

    Often I will listen to public radio on long drives when I am alone. Recently I happened to catch a program called "Don't Lecture Me" and it really caught my attention for several reasons. First, the speakers were all notable leaders in Physics Education Research such as Joe Redish, David Hestenes, and Eric Mazur. (See this month's WebSights column.) These folks are among many who have devoted their energies to understanding how students learn physics and how teachers can design classroom instruction and interactions to best meet the needs of learners. Second, on this particular trip, I had just observed a teacher whose class was very teacher-centered as the teacher lectured most of the class period. As we discussed this later, she expressed concern that she had to cover the material and didn't feel that she could do it without lecturing.

  3. EVALUATION OF SOME ANTIOXIDANTS’ EFFECTS IN KIDNEY HISTOLOGICAL STRUCTURE OF WEANED PIGS, INTOXICATED WITH DEOXYNIVALENOL (DON

    Directory of Open Access Journals (Sweden)

    GABI DUMITRESCU

    2013-12-01

    Full Text Available Deoxynivalenol is a mycotoxin produced by fungi of the Fusarium genera, which are abundant in various cereal crops and processed grains. In order to protect cell structure within the tissues studied, we tried various experimental variants by incorporating some selenium and boron sources into the combined fodder, and also in mixture with DON with different concentrations. To determine the morphophysiological changes induced by the mycotoxin DON upon kidney histological structure, and also the antioxidants’ effects, we took samples from 9 piglets belonging to three groups: group 1 (fed with fodder added with DON, group 2 (fed with fodder added with selenium and group 3 (fed with fodder added with selenium and DON. At renal parenchyma level, DON determines a series of changes in the renal corpuscles and also in the uriniferous tubes. These changes are represented by glomerular atrophies and nephrocyte epithelial dystrophies. The epithelial dystrophic processes occur in the renal medullar, too, where numerous ectasied capillaries and hemorrhagic areas are present. In the case of groups 2 and 3 consisted of piglets fed with fodder added with selenium, respectively fodder added with DON and selenium, the renal vascular network becomes hypertrophic associated with leukocyte infiltrative processes.

  4. Cytological changes in meristematic cells of Allium cepa L. root tip treated with extracts from callus of Catharanthus roseus (L. G. Don

    Directory of Open Access Journals (Sweden)

    Agnieszka Pietrosiuk

    2014-02-01

    Full Text Available The effect of an ethanolic extract from callus of Catharanthus roseus on Allium cepa root cells divisions was investigated. Two lines: white and green callus, were established on solid B5 medium with IAA 1 mg/l and kinetin 0.1 mg/l. The HPLC analysis of callus extracts showed the presence of indole alkaloids, however not known pharmacologically active alkaloids or derivatives used in semisynthesis (vinblastine, ajmalicine, serpentine, yohimbine, vindoline and catharanthine have been found. The ethanolic extract of C. roseus callus inhibited the number of mitoses in Allium cepa root tip cells. Short (1-3 hours treatment resulted in an increase in the index of late prophases, with characteristic light spaces, and the index of metaphases with twisted chromosomes forming an equatorial plate or irregular structures of c-metaphases. At the same time, the percentage of anaphases and telophases decreased significantly. Longer treatment of the root tip inhibited mitotic activity, stopping it completely already after 12 hours. Interphase nuclei became at first denser and homogeneous, eventually their structure became , partitioned into zones and formation of chromatin territories, with distinct large nucleoli has been observed. Electron microscope observations revealed well developed rough endoplasmic reticulum and thick, invaginating cell membrane.

  5. Don't Take This with That!

    Medline Plus

    Full Text Available ... Resources for You Special Features Don't take this with that! Share Tweet Linkedin Pin it More ... in the intestine that help absorb drugs. When this happens, less of the drug reaches the bloodstream ...

  6. Donning and doffing PPE for HPAI surveillance

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Instructions describing the proper donning and doffing personal protective equipment (PPE) for highly pathogenic avian influenza (HPAI) surveillance. This protocol...

  7. Dry Mouth? Don't Delay Treatment

    Science.gov (United States)

    ... For Consumers Home For Consumers Consumer Updates Dry Mouth? Don't Delay Treatment Share Tweet Linkedin Pin ... saliva, cavities may occur. back to top Dry Mouth Treatments Your doctor or dentist may recommend oral ...

  8. Don't Take This with That!

    Medline Plus

    Full Text Available ... Health and Human Services FDA U.S. Food and Drug Administration Protecting and Promoting Your Health A to ... Vaccines, Blood & Biologics Animal & Veterinary Cosmetics Tobacco Products Drugs Home Drugs Resources for You Special Features Don' ...

  9. Don Zhuanu otdajutsja tolko po ljubvi

    Index Scriptorium Estoniae

    2006-01-01

    Eestisse tuleb külla Roman Viktjuki Teater, 1. nov. mängitakse Vene Kultuurikeskuses Jean Genet' "Toatüdrukuid" (lavastaja Roman Viktjuk), 23. nov. etendatakse Eric-Emmanuel Scmitti "Don Juani viimast armastust" (lavastaja Roman Viktjuk)

  10. Don't Take This with That!

    Medline Plus

    Full Text Available HHS U.S. Department of Health and Human Services FDA U.S. Food and Drug Administration Protecting and Promoting ... Features Don't take this with that! Share Tweet Linkedin Pin ...

  11. Le paradigme du don face aux nouvelles réalités du monde du travail

    OpenAIRE

    Caillé, Alain

    2012-01-01

    Alain Caillé resitue le paradigme du don que le Mouvement anti-utilitariste en sciences sociales (MAUSS) s’emploie à dégager depuis une trentaine d’années avant de montrer comment les lunettes du don permettent de mieux voir ce qu’il y a dans la boîte noire des organisations et de mieux lire par là même les ravages du néomanagement. À cette contribution répond celle de Norbert Alter dans le cadre du dossier Chorus « Peut-on penser le travail par le don ? »

  12. Young CAS biologist gets five-year grant from Howard Hughes Medical Institute

    Institute of Scientific and Technical Information of China (English)

    2012-01-01

    Dr. TANG Chun, an outstanding young biologist from the CAS Wuhan Institute of Physics and Mathematics (WIPM) was announced in January 2012 to have won the first International Early Career Award from Howard Hughes Medical Institute with a five-year grant totaling up to 650,000 US dollars. Dr. Tang is an expert in using novel nuclear magnetic resonance methods to study the behavior and function of proteins,

  13. Educational background and professional participation by federal wildlife biologists: Implications for science, management, and The Wildlife Society

    Science.gov (United States)

    Schmutz, J.A.

    2002-01-01

    Over 2,000 people are employed in wildlife biology in the United States federal government. The size of this constituency motivated me to examine the amount of formal education federal biologists have received and the extent of continuing education they undertake by reading journals or attending scientific meetings. Most federal biologists who are members of The Wildlife Society (TWS) have a graduate degree. However, one-third have only a Bachelor of Science degree, despite the current trend toward hiring people with graduate degrees. Most federal biologists are not research biologists. Numbers of journals subscribed to was positively related to educational level. Less than one-third of all wildlife biologists employed by the United States Fish and Wildlife Service are members of TWS or subscribe to any of its journals. In contrast, the majority of presenters at the TWS 2000 Annual Conference were research biologists and members of TWS. The failure of many federal wildlife biologists to read scientific literature or attend professional meetings indicates a failure to promote the importance of continuing education in the federal workplace. I identify 2 potential adverse impacts of this failing: an inability to recognize important and relevant scientific contributions and an ineffectiveness in carrying out adaptive management.

  14. Speak Up! But Don't Strain Your Voice

    Science.gov (United States)

    ... Home Current Issue Past Issues Hearing Disorders Speak Up! But don't strain your voice Past Issues / ... Noise Exposure / How Loud Is Too Loud? / Speak Up! But don't strain your voice / Medical Mystery: ...

  15. Sleeping and Driving Don't Mix: Sleep Quiz

    Science.gov (United States)

    ... Don't Mix Sleeping and Driving Don't Mix Primary tabs View (active tab) Take if ($view_ ... Sign up for our Email Newsletter For Email Marketing you can trust Footer Contact Info Home | Contact | ...

  16. Fighting for life: Religion and science in the work of fish and wildlife biologists

    Science.gov (United States)

    Geffen, Joel Phillip

    Philosophers, historians, and sociologists of science have argued that it is impossible to separate fact from value. Even so, Americans generally demand that scientists be "objective." No bias is permitted in their work. Religious motivations in particular are widely considered anathema within the halls of science. My dissertation addresses both theoretical and practical aspects concerning objectivity in science through an examination of fish and wildlife biologists. I hypothesized that they use the language of objective science as a tool to convince others to protect habitats and species. Further, I claimed that this "rhetoric of science" is employed either consciously or unconsciously on behalf of personal values, and that religious and/or spiritual values figure significantly among these. Regarding the issue's practical applications, I argued in support of Susan Longino's assertion that while subjective influences exist in science, they do not necessarily indicate that objectivity has been sacrificed. My primary methodology is ethnographic. Thirty-five biologists working in the Pacific Northwest were interviewed during the course of summer 2001. Participant ages ranged from 23 to 78. Both genders were represented, as were various ethnic and cultural backgrounds, including Native American. I used a questionnaire to guide respondents through a consistent set of open-ended queries. I organized their answers under four categories: the true, the good, the beautiful, and the holy. The first three were borrowed from the theoretical writings of philosopher Immanuel Kant. The last came from Rudolf Otto's theological work. These categories provided an excellent analytical framework. I found that the great majority of fish and wildlife biologists strive for objectivity. However, they are also informed by powerful contextual values. These are derived from environmental ethics, aesthetic preferences pertaining to ecosystem appearance and function, and visceral experiences of

  17. The rise of repeal: policy entrepreneurship and Don't Ask, Don't Tell.

    Science.gov (United States)

    Neff, Christopher L; Edgell, Luke R

    2013-01-01

    We report on policy entrepreneurship by Servicemembers Legal Defense Network (SLDN) and how its legislative strategies used mini-windows of opportunity to shift Capitol Hill perspectives of Don't Ask, Don't Tell (DADT) from political plutonium to an emerging issue requiring a second look. Four phases in the legislative history of DADT are identified: radioactive, contested, emerging, and viable. In all, this article argues that SLDN's entrepreneurship focused on contesting congressional sensibilities to wait or defer on repeal, maintained that every discharge was damaging and transitioned toward a post-repeal mind set. Finally, we illustrate the importance of these transitions by comparing SLDN's 2004 estimated vote count for the introduction of the Military Readiness Enhancement Act with the final 2010 voting results on the Don't Ask, Don't Tell Repeal Act.

  18. Urinary deoxynivalenol (DON) and zearalenone (ZEA) as biomarkers of DON and ZEA exposure of pigs.

    Science.gov (United States)

    Thanner, S; Czeglédi, L; Schwartz-Zimmermann, H E; Berthiller, F; Gutzwiller, A

    2016-05-01

    Four diets contaminated with 1.1 to 5.0 mg/kg deoxynivalenol (DON) and 0.4 to 2.4 mg/kg zearalenone (ZEA) were fed to four groups of six growing Large White pigs. Urine samples were collected after 3 to 4 days and again after 6 to 7 days on the diets. On each sampling day, half of the animals were sampled in the morning, after an 8-h fast, and the other half were sampled in the afternoon, after 7 h of ad libitum access to feed. The urinary concentrations of DON, DON-glucuronide, DON-3-sulphate, de-epoxy-DON, as well as of ZEA, ZEA-14-glucuronide, α-zearalenol and α-zearalenol-14-glucuronide, analysed using LC-MS/MS, were used to calculate urinary DON and ZEA equivalent concentrations (DONe and ZEAe). The urinary concentration of DONe (P intake per kg body weight the day preceding sampling (r = 0.76 and 0.77; P intake during the 7 h preceding urine sampling in the afternoon and urinary DONe/creatinine ratio (r = 0.88) as well as between mean ZEA intake during 3 days preceding urine sampling and urinary ZEAe/creatinine ratio (r = 0.84) were even higher, reflecting the plasma elimination half-time of several hours for DON and of more than 3 days for ZEA. ZEAe analysed in enzymatically hydrolysed urine using an ELISA kit was highly correlated with the LC-MS/MS data (r = 0.94). The urinary DONe and ZEAe to creatinine ratios, analysed in pooled urine samples of several pigs fed the same diet, can be used to estimate their exposure to DON and ZEA. PMID:26888520

  19. Don't Ask, Don't Tell: A Costly and Wasteful Policy 

    OpenAIRE

    Barnes, Johnny L.

    2004-01-01

    Since the current policy known as "Don't Ask, Don't Tell" was adopted in the early 1990s, several changes have taken place which call into question the policy's validity. Firstly, the argument proponents of the ban use to justify it, namely that cohesion would suffer if admitted homosexuals were allowed to serve, has been undercut by social science analyses on the correlation between cohesion and performance. Their argument has also been undercut by empirical evidence from several nations tha...

  20. Don't Take This with That!

    Medline Plus

    Full Text Available ... En Español Search FDA Submit search Popular Content Home Food Drugs Medical Devices Radiation-Emitting Products Vaccines, Blood & Biologics Animal & Veterinary Cosmetics Tobacco Products Drugs Home Drugs Resources for You Special Features Don't ...

  1. Speaking Personally--With Don Knezek

    Science.gov (United States)

    American Journal of Distance Education, 2009

    2009-01-01

    This article presents an interview with Don Knezek, CEO of International Society for Technology in Education (ISTE). Knezek is a leader in coupling technology and creativity to transform education. His experience and impact span local to global levels--ranging from district-wide, classroom-based innovation and far-reaching, multi-state projects to…

  2. Don Juan on manipulaator / Kai Ilustrumm

    Index Scriptorium Estoniae

    Ilustrumm, Kai

    2004-01-01

    Nüüdisaegne muusikalis-koreograafiline fantaasia "Don Juani pidusöök" Kanuti Gildi Saalis. Eesti tantsijate ja hollandi muusikute Holland Windplayers koostöös sündinud projekti tutvustab Tallinna Filharmoonia sarja "Diplomaadilised noodid" juht Heli Vaus-Tamm

  3. A Conversation with Don Knuth: Part I.

    Science.gov (United States)

    Albers, Donald J.; Steen, Lynn Arthur

    1982-01-01

    This interview reviews Don Knuth's life and contributions and his views on a variety of mathematical and computer-related topics. He is viewed as the pre-eminent scholar of computer science in the world, and is probably best known for a series of books titled, "The Art of Computer Programing." (MP)

  4. Don Quijote en Canadá

    Directory of Open Access Journals (Sweden)

    Cristina Botella González

    2007-01-01

    Full Text Available Conocer a Cervantes y su obra.- Descubrir otros países y artistas.- Conocer al personaje de Don Quijote.- Familiarizarse con los personajes principales.- Relacionar la pintura y la literatura.- Desarrollar habilidades manuales y artísticas.- Potenciar la creatividad.

  5. A FASTQ compressor based on integer-mapped k-mer indexing for biologist.

    Science.gov (United States)

    Zhang, Yeting; Patel, Khyati; Endrawis, Tony; Bowers, Autumn; Sun, Yazhou

    2016-03-15

    Next generation sequencing (NGS) technologies have gained considerable popularity among biologists. For example, RNA-seq, which provides both genomic and functional information, has been widely used by recent functional and evolutionary studies, especially in non-model organisms. However, storing and transmitting these large data sets (primarily in FASTQ format) have become genuine challenges, especially for biologists with little informatics experience. Data compression is thus a necessity. KIC, a FASTQ compressor based on a new integer-mapped k-mer indexing method, was developed (available at http://www.ysunlab.org/kic.jsp). It offers high compression ratio on sequence data, outstanding user-friendliness with graphic user interfaces, and proven reliability. Evaluated on multiple large RNA-seq data sets from both human and plants, it was found that the compression ratio of KIC had exceeded all major generic compressors, and was comparable to those of the latest dedicated compressors. KIC enables researchers with minimal informatics training to take advantage of the latest sequence compression technologies, easily manage large FASTQ data sets, and reduce storage and transmission cost. PMID:26743127

  6. [Discreet follies. Variations on Don Quixote's folly].

    Science.gov (United States)

    Gracia Guillén, Diego

    2005-01-01

    Physicians and psychiatrists have been discussing through centuries about the madness of Don Quixote. The first in doing that was Philippe Pinel, followed immediately by many others. They all agreed in diagnosing Don Quixote as "monomaniac". Cervantes was considered an excellent "nosographer", and the novel a paradigmatic description of this mental disorder. After the Kraepelin's work, the old Pinelian nosography was substituted by a new system of classification of mental diseases, and Don Quixote was considered "paranoid". Finally, after the DSM IV, the usual diagnostic is "delusional disorder". Throughout history there have been two different types of mental disorders, being only one of them considered pathological. This difference appears in English in words like "crazy" and "mad." The thesis of this essay is that Don Quixote is more a crazy than a mad man. He is trying to achieve a world full of peace, justice and love. His moral ideal is to make possible the triumph of this perfect world. This crazy goal was deeply influenced by the Erasmian ideology. And Erasmus of Rotterdam was the author of a very famous book entitled The Praise of Folly, published the year 1509, almost a century before the novel of Cervantes. In this work, the word opposite to "loco" (crazy, mad, insane) is "discreto", a word of difficult translation to English, which means at the same time "shrewd," "discreet" and "ingenious". Cervantes says at the beginning of the second part of his work, that Don Quixote is doing in it "discretas locuras" ("discreet follies", trans. Thomas Shelton, 1620; "ingenious follies", trans. Tobias G. Smollet, 1755; "shrewd lunacies", trans. John Ormsby, 1885). This mixture of cleverness and lack of moderation is considered by the author the interpretation's key of the work.

  7. Early Activation of MAPK p44/42 Is Partially Involved in DON-Induced Disruption of the Intestinal Barrier Function and Tight Junction Network.

    Science.gov (United States)

    Springler, Alexandra; Hessenberger, Sabine; Schatzmayr, Gerd; Mayer, Elisabeth

    2016-01-01

    Deoxynivalenol (DON), produced by the plant pathogens Fusarium graminearum and Fusarium culmorum, is one of the most common mycotoxins, contaminating cereal and cereal-derived products. Although worldwide contamination of food and feed poses health threats to humans and animals, pigs are particularly susceptible to this mycotoxin. DON derivatives, such as deepoxy-deoxynivalenol (DOM-1), are produced by bacterial transformation of certain intestinal bacteria, which are naturally occurring or applied as feed additives. Intestinal epithelial cells are the initial barrier against these food- and feed-borne toxins. The present study confirms DON-induced activation of MAPK p44/42 and inhibition of p44/42 by MAPK-inhibitor U0126 monoethanolate. Influence of DON and DOM-1 on transepithelial electrical resistance (TEER), viability and expression of seven tight junction proteins (TJ), as well as the potential of U0126 to counteract DON-induced effects, was assessed. While DOM-1 showed no effect, DON significantly reduced TEER of differentiated IPEC-J2 and decreased expression of claudin-1 and -3, while leaving claudin-4; ZO-1, -2, and -3 and occludin unaffected. Inhibition of p44/42 counteracted DON-induced TEER decrease and restored claudin-3, but not claudin-1 expression. Therefore, effects of DON on TEER and claudin-3 are at least partially p44/42 mediated, while effects on viability and claudin-1 are likely mediated via alternative pathways. PMID:27618100

  8. Early Activation of MAPK p44/42 Is Partially Involved in DON-Induced Disruption of the Intestinal Barrier Function and Tight Junction Network

    Science.gov (United States)

    Springler, Alexandra; Hessenberger, Sabine; Schatzmayr, Gerd; Mayer, Elisabeth

    2016-01-01

    Deoxynivalenol (DON), produced by the plant pathogens Fusarium graminearum and Fusarium culmorum, is one of the most common mycotoxins, contaminating cereal and cereal-derived products. Although worldwide contamination of food and feed poses health threats to humans and animals, pigs are particularly susceptible to this mycotoxin. DON derivatives, such as deepoxy-deoxynivalenol (DOM-1), are produced by bacterial transformation of certain intestinal bacteria, which are naturally occurring or applied as feed additives. Intestinal epithelial cells are the initial barrier against these food- and feed-borne toxins. The present study confirms DON-induced activation of MAPK p44/42 and inhibition of p44/42 by MAPK-inhibitor U0126 monoethanolate. Influence of DON and DOM-1 on transepithelial electrical resistance (TEER), viability and expression of seven tight junction proteins (TJ), as well as the potential of U0126 to counteract DON-induced effects, was assessed. While DOM-1 showed no effect, DON significantly reduced TEER of differentiated IPEC-J2 and decreased expression of claudin-1 and -3, while leaving claudin-4; ZO-1, -2, and -3 and occludin unaffected. Inhibition of p44/42 counteracted DON-induced TEER decrease and restored claudin-3, but not claudin-1 expression. Therefore, effects of DON on TEER and claudin-3 are at least partially p44/42 mediated, while effects on viability and claudin-1 are likely mediated via alternative pathways. PMID:27618100

  9. Urinary deoxynivalenol (DON) and zearalenone (ZEA) as biomarkers of DON and ZEA exposure of pigs.

    Science.gov (United States)

    Thanner, S; Czeglédi, L; Schwartz-Zimmermann, H E; Berthiller, F; Gutzwiller, A

    2016-05-01

    Four diets contaminated with 1.1 to 5.0 mg/kg deoxynivalenol (DON) and 0.4 to 2.4 mg/kg zearalenone (ZEA) were fed to four groups of six growing Large White pigs. Urine samples were collected after 3 to 4 days and again after 6 to 7 days on the diets. On each sampling day, half of the animals were sampled in the morning, after an 8-h fast, and the other half were sampled in the afternoon, after 7 h of ad libitum access to feed. The urinary concentrations of DON, DON-glucuronide, DON-3-sulphate, de-epoxy-DON, as well as of ZEA, ZEA-14-glucuronide, α-zearalenol and α-zearalenol-14-glucuronide, analysed using LC-MS/MS, were used to calculate urinary DON and ZEA equivalent concentrations (DONe and ZEAe). The urinary concentration of DONe (P < 0.001), but not of ZEAe (P = 0.31), was lower in the fasted than that in the fed animals. The urinary DONe/creatinine and ZEAe/creatinine ratios were highly correlated with DON and ZEA intake per kg body weight the day preceding sampling (r = 0.76 and 0.77; P < 0.001). The correlations between DON intake during the 7 h preceding urine sampling in the afternoon and urinary DONe/creatinine ratio (r = 0.88) as well as between mean ZEA intake during 3 days preceding urine sampling and urinary ZEAe/creatinine ratio (r = 0.84) were even higher, reflecting the plasma elimination half-time of several hours for DON and of more than 3 days for ZEA. ZEAe analysed in enzymatically hydrolysed urine using an ELISA kit was highly correlated with the LC-MS/MS data (r = 0.94). The urinary DONe and ZEAe to creatinine ratios, analysed in pooled urine samples of several pigs fed the same diet, can be used to estimate their exposure to DON and ZEA.

  10. ASPECTS CONCERNING THE EFFECT OF SOME ANTIOXIDANTS ON LIVER HISTOLOGICAL STRUCTURE IN WEANED PIGS, INTOXICATED WITH DEOXYNIVALENOL (DON

    Directory of Open Access Journals (Sweden)

    GABI DUMITRESCU

    2013-12-01

    Full Text Available Deoxynivalenol is a mycotoxin produced by fungi of the Fusarium genera, which are abundant in various cereal crops and processed grains. In order to protect cell structure within the tissues studied, we tried various experimental variants by incorporating some selenium and boron sources into the combined fodder, and also in mixture with DON with different concentrations. To determine the morphophysiological changes induced by the mycotoxin DON upon liver histological structure, and also the antioxidants’ effects, we took samples from 9 piglets belonging to three groups: group 1 (fed with fodder added with DON, group 2 (fed with fodder added with selenium and group 3 (fed with fodder added with selenium and DON. In the case of the individuals in groups 2 and 3 fed with fodder added with addition of selenium, respectively of DON and selenium, the microscopic images reveal the presence of uniform hepatic lobules, delimited between them through fine conjunctive septa. The intralobular capillary network is hypertrophic. The selenium added in the fodder for the individuals from group 3 has a hepatoprotective role, aspect suggested by dystrophic processes occurred on small areas, caused by the presence of DON and by the vascular network’s hypertrophy. In the case of this group, we observed a strong stimulation on the defense system, suggested by the presence of the leukocyte infiltrates within the interlobular conjunctive and by the vascular network’s hypertrophy.

  11. What in silico molecular docking can do for the `bench-working biologists'

    Indian Academy of Sciences (India)

    Marius Mihăşan

    2012-12-01

    Required by an increasing amount of scientists, the in silico docking field is in full expansion, new algorithms and methods appearing at an exponential rate. The sheer range of available programs is overwhelming for the bench-working biologist, which is often discouraging by the lack of a graphical user interface, good user manual or literature to validate a given program. This mini-review attempts to present the docking problem and available solutions from a non-bioinformatician point of view and makes a selection of the available servers and programs. These tools are evaluated from several points of view, as numbers of citations, ease of usage and computer requirements. Finally, the capabilities and limitations as well as specific applications of in silico docking techniques are presented.

  12. BioLogistics and the Struggle for Efficiency: Concepts and Perspectives

    CERN Document Server

    Helbing, Dirk; Diez, Stefan; Peters, Karsten; Kalaidzidis, Yannis; Padberg, Kathrin; Lammer, Stefan; Johansson, Anders; Breier, Georg; Schulze, Frank; Zerial, Marino; 10.1142/S0219525909002374

    2010-01-01

    The growth of world population, limitation of resources, economic problems and environmental issues force engineers to develop increasingly efficient solutions for logistic systems. Pure optimization for efficiency, however, has often led to technical solutions that are vulnerable to variations in supply and demand, and to perturbations. In contrast, nature already provides a large variety of efficient, flexible and robust logistic solutions. Can we utilize biological principles to design systems, which can flexibly adapt to hardly predictable, fluctuating conditions? We propose a bio-inspired "BioLogistics" approach to deduce dynamic organization processes and principles of adaptive self-control from biological systems, and to transfer them to man-made logistics (including nanologistics), using principles of modularity, self-assembly, self-organization, and decentralized coordination. Conversely, logistic models can help revealing the logic of biological processes at the systems level.

  13. GeneNotes – A novel information management software for biologists

    Directory of Open Access Journals (Sweden)

    Wong Wing H

    2005-02-01

    Full Text Available Abstract Background Collecting and managing information is a challenging task in a genome-wide profiling research project. Most databases and online computational tools require a direct human involvement. Information and computational results are presented in various multimedia formats (e.g., text, image, PDF, word files, etc., many of which cannot be automatically processed by computers in biologically meaningful ways. In addition, the quality of computational results is far from perfect and requires nontrivial manual examination. The timely selection, integration and interpretation of heterogeneous biological information still heavily rely on the sensibility of biologists. Biologists often feel overwhelmed by the huge amount of and the great diversity of distributed heterogeneous biological information. Description We developed an information management application called GeneNotes. GeneNotes is the first application that allows users to collect and manage multimedia biological information about genes/ESTs. GeneNotes provides an integrated environment for users to surf the Internet, collect notes for genes/ESTs, and retrieve notes. GeneNotes is supported by a server that integrates gene annotations from many major databases (e.g., HGNC, MGI, etc.. GeneNotes uses the integrated gene annotations to (a identify genes given various types of gene IDs (e.g., RefSeq ID, GenBank ID, etc., and (b provide quick views of genes. GeneNotes is free for academic usage. The program and the tutorials are available at: http://bayes.fas.harvard.edu/genenotes/. Conclusions GeneNotes provides a novel human-computer interface to assist researchers to collect and manage biological information. It also provides a platform for studying how users behave when they manipulate biological information. The results of such study can lead to innovation of more intelligent human-computer interfaces that greatly shorten the cycle of biology research.

  14. Sleep and sleep disorders in Don Quixote.

    Science.gov (United States)

    Iranzo, Alex; Santamaria, Joan; de Riquer, Martín

    2004-01-01

    In Don Quijote de la Mancha, Miguel de Cervantes presents Don Quixote as an amazing character of the 17th century who suffers from delusions and illusions, believing himself to be a medieval knight errant. Besides this neuropsychiatric condition, Cervantes included masterful descriptions of several sleep disorders such as insomnia, sleep deprivation, disruptive loud snoring and rapid eye movement sleep behaviour disorder. In addition, he described the occurrence of physiological, vivid dreams and habitual, post-prandial sleepiness--the siesta. Cervantes' concept of sleep as a passive state where all cerebral activities are almost absent is in conflict with his description of abnormal behaviours during sleep and vivid, fantastic dreams. His concept of sleep was shared by his contemporary, Shakespeare, and could have been influenced by the reading of the classical Spanish book of psychiatry Examen de Ingenios (1575).

  15. Polarized Light Experiment, Presa Don Martin, Mexico

    Science.gov (United States)

    1989-01-01

    This is a single scene from a pair (frames 021 and 024) to study the effects of polarized light in Earth Observations. One scene was exposed with vertically polarized light, the other, horizontally. The subject in this study, is a lake behind Presa (dam) Don Martin (27.5N, 100.5W) on thge edge of the Rio Grande Plain near it's boundry with the Sierra Madre Oriental in Coahuila, Mexico.

  16. Don quijote, Christelike geloof en natuurwetenskap 1

    OpenAIRE

    H.P.P. Lötter

    1987-01-01

    The story about Don Qulchote and the windmills is used in this article as an analogy to typify the debates that have taken place in history between natural science and representatives of Christian religion. The analogy is tested on debates that took place around Galileo. Darwin, Newton, Cuvier and the Big Bang theory. The analogy matches these debates quite well and this Is explained by the view that scientific claims to knowledge, and claims to knowledge based on Christian faith, belong to d...

  17. Protien expression profiling of mouse thymoma upon exposure to the tricothecene deoxynivalenol (DON) Implications for its mechanism of action

    NARCIS (Netherlands)

    Osman, A.M.; Pennings, J.L.A.; Blokland, M.H.; Peijnenburg, A.A.C.M.; Loveren, van H.

    2010-01-01

    The objective of this work was to investigate whether proteomic analysis of thymoma cells treated with the trichothecene deoxynivalenol (DON) as compared to non-treated (control) cells would reveal differential protein expression, and thus would contribute to a better understanding of the mechanisms

  18. Don't be such a scientist talking substance in an age of style

    CERN Document Server

    Olson, Randy

    2009-01-01

    How to relate scientific information in a way that entertains and enlightens? After twenty years as a marine biologist, a Ph.D. from Harvard, a tenured professorship at the University of New Hampshire, and more than twenty published research papers, why is Dr. Randy Olson telling readers, 'Don't Be Such a Scientist'? The hard-earned advice comes from a fifteen-year career transition to filmmaking, culminating in the acclaimed documentaries "Flock of Dodos: The Evolution-Intelligent Design Circus" and "Sizzle: A Global Warming Comedy". In his first book, Olson makes clear what those glued to their microscopes and climate models may not recognize: the general public doesn't speak science. For better or worse, most of us rely not on intellect, but on what Olson terms the organs of mass communication: the heart, the gut, and even 'the lower organs'. Why else do some still question the existence of global warming or evolution when the evidence is irrefutable? Drawing on his own hilarious - and at times humiliating...

  19. Conscience dilemma: to become a bioengineer or to survive as a biologist.

    Science.gov (United States)

    Selimoglu, Sureyya Mert

    2014-01-01

    Bioengineering is the consideration of biological problems from modern engineering, therefore money-oriented, perspective. Today, grant-giving bodies always favor bioengineering projects rather than pure biology projects (like those in ecology, entomology, etc.). Therefore, today's biologist is forced to be on the horns of a dilemma. They have to either submit a very powerful and valid reason for the proposal of their project, or change the project to one having a potential of money-based outcome. On the other hand, because of dealing with the living components of nature, conducting a research in pure biology is like a kind of worship. For this reason, from a believer scientist's view, a deviation (in terms of research) from biology to bioengineering can be considered like committing a sin. Unfortunately, today's wild capitalism has been bringing new sinners day by day, and this system will continue for the foreseeable future unless grant-giving bodies comprehend the real importance of pure biology. PMID:25936219

  20. Measuring, interpreting, and responding to changes in coral reefs: A challenge for biologists, geologist, and managers

    Science.gov (United States)

    Rogers, Caroline S.; Miller, Jeff; Hubbard, Dennis K.; Rogers, Caroline S.; Lipps, Jere H.; Stanley, George D.

    2016-01-01

    What, exactly, is a coral reef? And how have the world’s reefs changed in the last several decades? What are the stressors undermining reef structure and function? Given the predicted effects of climate change, do reefs have a future? Is it possible to “manage” coral reefs for resilience? What can coral reef scientists contribute to improve protection and management of coral reefs? What insights can biologists and geologists provide regarding the persistence of coral reefs on a human timescale? What is reef change to a biologist… to a geologist?Clearly, there are many challenging questions. In this chapter, we present some of our thoughts on monitoring and management of coral reefs in US national parks in the Caribbean and western Atlantic based on our experience as members of monitoring teams. We reflect on the need to characterize and evaluate reefs, on how to conduct high-quality monitoring programs, and on what we can learn from biological and geological experiments and investigations. We explore the possibility that specific steps can be taken to “manage” coral reefs for greater resilience.

  1. Reading "Las Meninas": An Ekphrastic Approach to Teaching "Don Quijote"

    Science.gov (United States)

    Ortuno, Marian

    2012-01-01

    Reading and teaching "Don Quijote" present multiple challenges to twenty-first century students and instructors who are culturally and historically distanced from the seventeenth century. With "Las Meninas" serving as a visual lexicon for cuing correlative themes and events in "Don Quijote", the instructor, through an ekphrastic, interdisciplinary…

  2. Le paradigme du don face aux nouvelles réalités du monde du travail The gift paradigm facing new realities in the work world El paradigma del don frente a las nuevas realidades del mundo del trabajo

    OpenAIRE

    Alain Caillé

    2012-01-01

    Alain Caillé resitue le paradigme du don que le Mouvement anti-utilitariste en sciences sociales (MAUSS) s’emploie à dégager depuis une trentaine d’années avant de montrer comment les lunettes du don permettent de mieux voir ce qu’il y a dans la boîte noire des organisations et de mieux lire par là même les ravages du néomanagement. À cette contribution répond celle de Norbert Alter dans le cadre du dossier Chorus « Peut-on penser le travail par le don ? »Alain Caillé starts by resituating th...

  3. Cognitive empathy in inter-disciplinary research: the contrasting attitudes of plant breeders and molecular biologists towards rice

    Indian Academy of Sciences (India)

    E Haribabu

    2000-12-01

    I draw attention to the perceptions of and interactions between molecular biologists and scientists engaged in plant breeding in India, who have been attempting to employ molecular biology tools to understand and intervene to improve the rice crop. The present essay suggests that the concept of cognitive empathy is crucial for enabling basic scientists and applied scientists to begin to understand phenomena from the point of view of the other and from the point of view of society at large, and in arriving at solutions that are scientifically feasible and socially acceptable. Socialization into disciplinary cultures, organizational factors and individual anxieties seem to inhibit inter-disciplinary collaboration. The majority of rice breeders and a small group of molecular biologists emphasize the relative merits of marker-assisted selection (MAS) in the near term vis-à-vis the currently controversial transgenic approach for rice crop improvement in India.

  4. Don quijote, Christelike geloof en natuurwetenskap 1

    Directory of Open Access Journals (Sweden)

    H. P.P. Lötter

    1987-03-01

    Full Text Available The story about Don Qulchote and the windmills is used in this article as an analogy to typify the debates that have taken place in history between natural science and representatives of Christian religion. The analogy is tested on debates that took place around Galileo. Darwin, Newton, Cuvier and the Big Bang theory. The analogy matches these debates quite well and this Is explained by the view that scientific claims to knowledge, and claims to knowledge based on Christian faith, belong to different categories and are thus not concurrents for the same truth. Gilbert Ryle's criticism on Descartes' mind-body dualism is used here to strengthen this argument. The article is concluded by pointing out in what ways one can engage in a meaningful debate on the nature of the relationship between science and Christian religion.

  5. We don't need another hero.

    Science.gov (United States)

    Badaracco, J L

    2001-09-01

    Everybody loves the stories of heroes like Martin Luther King, Jr., Mother Teresa, and Gandhi. But the heroic model of moral leadership usually doesn't work in the corporate world. Modesty and restraint are largely responsible for the achievements of the most effective moral leaders in business. The author, a specialist in business ethics, says the quiet leaders he has studied follow four basic rules in meeting ethical challenges and making decisions. The rules constitute an important resource for executives who want to encourage the development of such leaders among their middle managers. The first rule is "Put things off till tomorrow." The passage of time allows turbulent waters to calm and lets leaders' moral instincts emerge. "Pick your battles" means that quiet leaders don't waste political capital on fights they can't win; they save it for occasions when they really want to fight. "Bend the rules, don't break them" sounds easier than it is--bending the rules in order to resolve a complicated situation requires imagination, discipline, restraint, flexibility, and entrepreneurship. The fourth rule, "Find a compromise," reflects the author's finding that quiet leaders try not to see situations as polarized tests of ethical principles. These individuals work hard to craft compromises that are "good enough"--responsible and workable enough--to satisfy themselves, their companies, and their customers. The vast majority of difficult problems are solved through the consistent striving of people working far from the limelight. Their quiet approach to leadership doesn't inspire, thrill, or provide story lines for uplifting TV shows. But the unglamorous efforts of quiet leaders make a tremendous difference every day in the corporate world.

  6. We don't need another hero.

    Science.gov (United States)

    Badaracco, J L

    2001-09-01

    Everybody loves the stories of heroes like Martin Luther King, Jr., Mother Teresa, and Gandhi. But the heroic model of moral leadership usually doesn't work in the corporate world. Modesty and restraint are largely responsible for the achievements of the most effective moral leaders in business. The author, a specialist in business ethics, says the quiet leaders he has studied follow four basic rules in meeting ethical challenges and making decisions. The rules constitute an important resource for executives who want to encourage the development of such leaders among their middle managers. The first rule is "Put things off till tomorrow." The passage of time allows turbulent waters to calm and lets leaders' moral instincts emerge. "Pick your battles" means that quiet leaders don't waste political capital on fights they can't win; they save it for occasions when they really want to fight. "Bend the rules, don't break them" sounds easier than it is--bending the rules in order to resolve a complicated situation requires imagination, discipline, restraint, flexibility, and entrepreneurship. The fourth rule, "Find a compromise," reflects the author's finding that quiet leaders try not to see situations as polarized tests of ethical principles. These individuals work hard to craft compromises that are "good enough"--responsible and workable enough--to satisfy themselves, their companies, and their customers. The vast majority of difficult problems are solved through the consistent striving of people working far from the limelight. Their quiet approach to leadership doesn't inspire, thrill, or provide story lines for uplifting TV shows. But the unglamorous efforts of quiet leaders make a tremendous difference every day in the corporate world. PMID:11550628

  7. Odgojno očinstvo. Don Boscova pouka

    OpenAIRE

    Giraudo, Aldo

    2007-01-01

    Podsjećajući na autobiografski don Boscov zapis o bolnom prisjećanju na smrt vlastita oca u ranom djetinjstvu, autor razmišlja o don Boscu kao duhovnom ocu mladih. Očinski lik ima važnu ulogu u don Boscovu odgajateljskom životu i djelovanju. U njegovu odgojnom sustavu posebno mjesto zauzima empatijska suosjećajnost s odgajanikom. Tako se između odgajatelja i odgajanika razvija specifična komunikacija po kojoj se odgajanika upućuje na prikladan put prema njemu svojstvenom rastu i zrelosti. Odg...

  8. Associação entre carcinoma espinocelular de cabeça e pescoço e polimorfismo no p53 (códon 72 P53 codon 72 polymorphism association with head and neck squamous cell carcinoma

    Directory of Open Access Journals (Sweden)

    Zahra Mojtahedi

    2010-06-01

    Full Text Available O gene supressor tumoral p53 abriga um polimorfismo funcional que codifica ou arginina (Arg ou prolina (Pro no códon 72 da proteína p53. Este polimorfismo tem sido considerado associado com o desenvolvimento e prognóstico do carcinoma espinocelular de cabeça e pescoço (CECP. OBJETIVO: Foram avaliados genótipo e freqüências alélicas do códon 72 do p53 em pacientes iranianos com CECP. TIPO DE ESTUDO: Estudo de Caso. MATERIAIS E MÉTODOS: Um total de 132 pacientes com CECP e 123 controles saudáveis foram genotipados. A fonte de DNA foi composta de células mononucleares do sangue periférico. A amplificação do DNA foi realizada através da reação em cadeia da polimerase específica para alelos. RESULTADOS: A distribuição dos alelos e genótipos não foi significativamente diferente entre os pacientes e controles. Além disso, nenhuma associação estatisticamente significativa foi encontrada entre o polimorfismo do códon 72 do p53 e localização, sexo ou idade no momento do diagnóstico. No entanto, o genótipo Pro/Pro estava significativamente aumentado em pacientes no estágio IV (30,8% quando comparado ao estágio I-III da doença (11,1% (p=0,03, e um número significativamente maior de doentes com o alelo Pro teve um aumento no risco de desenvolver doença no estágio IV (OR=2,2, IC= 95% =1.2-4.2, p=0,01. CONCLUSÃO: Os dados revelaram que o polimorfismo do p53 não afeta o risco de CECP em iranianos; porém, pode afetar a progressão para um estágio superior tumor.p53 tumoral suppressor gene harbors a functional polymorphism which codes either arginine (Arg or proline (Pro in the protein p53 of codon 72. Such polymorphism has been associated with the development or prognosis of head and neck squamous cell carcinoma (HNSCC. AIM: we assessed codon 72 p53 allelic frequencies and genotypes in HNSCC Iranian patients. STUDY DESIGN: Case Study. MATERIALS AND METHODS: a total of 132 HNSCC patients and 123 healthy controls were

  9. Progeria: translational insights from cell biology.

    Science.gov (United States)

    Gordon, Leslie B; Cao, Kan; Collins, Francis S

    2012-10-01

    Cell biologists love to think outside the box, pursuing many surprising twists and unexpected turns in their quest to unravel the mysteries of how cells work. But can cell biologists think outside the bench? We are certain that they can, and clearly some already do. To encourage more cell biologists to venture into the realm of translational research on a regular basis, we would like to share a handful of the many lessons that we have learned in our effort to develop experimental treatments for Hutchinson-Gilford progeria syndrome (HGPS), an endeavor that many view as a "poster child" for how basic cell biology can be translated to the clinic.

  10. Don't Blame Kids' Behavior on Full Moon

    Science.gov (United States)

    ... html Don't Blame Kids' Behavior on Full Moon International study puts common myth to rest To ... parents swear their children's behavior changes when the moon is full, but new research suggests otherwise. "Our ...

  11. Common Culex Mosquitoes Don't Transmit Zika Virus

    Science.gov (United States)

    ... 161186.html Common Culex Mosquitoes Don't Transmit Zika Virus: Study These widespread insects do spread West Nile ... t appear to be able to transmit the Zika virus to people, researchers report. The researchers at Kansas ...

  12. News from the CFHT/ESPaDOnS spectropolarimeter

    Science.gov (United States)

    Moutou, C.; Malo, L.; Manset, N.; Selliez-Vandernotte, L.; Desrochers, M.-E.

    2015-12-01

    The ESPaDOnS spectropolarimeter has been in use on the Canada-France-Hawaii Telescope (CFHT) since 2004, for studying stars, galactic objects and planets. ESPaDOnS is used in queued service observing mode since 2008, which allows an optimization of the science outcome. In this article, we summarize the new functionalities and analyses made on ESPaDOnS operations and data for the present and future users. These modifications include: signal-to-noise ratio based observing, radial velocity nightly drifts, the OPERA pipeline under development, the measurement of H2O content in the Maunakea sky, and the use of ESPaDOnS with the neighbour telescope Gemini.

  13. Taking a Bike Ride? Don't Forget Your Helmet

    Science.gov (United States)

    ... medlineplus.gov/news/fullstory_160423.html Taking a Bike Ride? Don't Forget Your Helmet Wearing the ... 13, 2016 (HealthDay News) -- All cyclists should wear bike helmets, which can reduce the risk of head ...

  14. Hyperbaric Oxygen Therapy: Don't Be Misled

    Science.gov (United States)

    ... For Consumers Home For Consumers Consumer Updates Hyperbaric Oxygen Therapy: Don't Be Misled Share Tweet Linkedin ... HBOT Works What are the Risks No, hyperbaric oxygen therapy (HBOT) has not been clinically proven to ...

  15. Will 3552 Don Quixote escape from the Solar System?

    Directory of Open Access Journals (Sweden)

    Suryadi Siregar

    2011-05-01

    Full Text Available Asteroid 1983 SA, well known as 3552 Don Quixote, is one of Near Earth Asteroids (NEAs which is the most probable candidate for the cometary origin, or otherwise as Jupiter-Family-Comets (JFCs. The aim of this study is to investigate the possibility of 3552 Don Quixote to be ejected from the Solar System. This paper presents an orbital evolution of 100 hypothetical asteroids generated by cloning 3552 Don Quixote. Investigation of its orbital evolution is conducted by using the SWIFT subroutine package, where the gravitational perturbations of eight major planets in the Solar System are considered. Over very short time scales (220 kyr relative to the Solar System life time (10 Gyr, the asteroid 3552 Don Quixote gave an example of chaotic motion that can cause asteroid to move outward and may be followed by escaping from the Solar System. Probability of ejection within the 220 kyr time scale is 50%.

  16. Evaluation of effect of measures taken after a DON outbreak.

    Science.gov (United States)

    Spanjer, M C; Scholten, J M; Rensen, P M

    2002-03-01

    In April 1999 an amount of 2600 μg/kg DON was found in a sample breakfast cereals in the Netherlands. This event was the start of a lot of activities, which dealt with the prevention, control, health and consumer aspects of DON in food for human consumption. The Food Inspection Services started a monitoring program to measure DON in cereal products, flour and raw cereals. The National Institute of Public Health and the Environment, another part of the Ministry of Health in the Netherlands, was asked to carry out a risk analysis on DON. This was the basis for the Minister of Health to set an action limit for consumer products. She also informed Brussels and asked for a European limit. The Main Board on Agriculture set out to implement measures to be taken at harvesting, milling and bread baking industry. The Scientific Committee on Food of the EC expressed an opinion on DON in December 1999. Worldwide attention leads to discussion of a DON limit by JECFA in February 2001.In the period may 1999 until march 2002 a number of more than 1700 samples were analysed on DON. These originated from the cereal harvest of the years 1998 until 2001. The results showed a sharp decrease of DON content in samples of harvest 1999 when compared to 1998. This lower level was maintained in the 2000 and 2001 harvests. Apparently the measures taken to control the DON level succeeded to maintain values below the action limits. Despite these activities a smaller outbreak of DON appeared in 2001 in pasta products at a lower extent. This indicated that control should be done systematically, not sporadically, and at a European level, which is made possible since EC has set a limit in July 2000. Analytical results of the measurements are presented, together with the chronological order of the associated activities of national, EU and worldwide bodies on human health control. Special attention is paid to DON in bread, related to the level in flour.

  17. Stem cells: a plant biology perspective

    NARCIS (Netherlands)

    Scheres, B.J.G.

    2005-01-01

    A recent meeting at the Juan March Foundation in Madrid, Spain brought together plant biologists to discuss the characteristics of plant stem cells that are unique and those that are shared by stem cells from the animal kingdom

  18. Don Quijote, el escribidor y el escritor

    Directory of Open Access Journals (Sweden)

    Giovanna Pollarolo

    2010-06-01

    Full Text Available In his diverse critical studies, Mario Vargas Llosa has shown particular interest in reflecting on Miguel de Cervantes’s novelistic theory in accordance with his own concerns as a scholar and novelist. Suffice it to read the essay “Una novela para el siglo XXI” that he wrote in 2005 as an introduction to the edition of Don Quijote prepared to celebrate the IV Centenario, to make sure that Vargas Llosa “reads” Cervantes in accordance with his own reflections on the theory of the novel, fanaticism or the “insanity” of the characters, the fiction and its relationship with “reality”, the narrative voices or the problem of the narrator. But the Cervantes – Vargas Llosa relationship goes beyond the theoretical level or reflective and is manifested in his own novelistic creation such as ocurrs, specifically, in La tía Julia y el escribidor. In that sense, the main goal of this study is to analyze the “dialogue” that is set between La tía Julia y el escribidor and the Quijotefrom the point of view of the “insanity” of Pedro Camacho, the writer of radio soap operas created by Vargas Llosa, and their similarities with that of the knight-errant.

  19. Don't neglect routine staff meetings.

    Science.gov (United States)

    Board, H K

    1982-03-01

    Staff meetings are essential to good staff communication. Meetings help keep the grapevine from growing so big that it strangles the group with its rumors. By holding regular meetings with your staff, you create a consistency in your communications that helps prevent problems that you don't even suspect from cropping up. All personnel should attend the meetings. This way everyone hears news at the same time. Be consistent in your use of meetings. Meetings are more effective if you have a planned agenda and a firm time schedule. Encourage your staff to use meetings to talk out problems that affect the group. Once the meeting is over, encourage them to leave their feelings in the room. Many leaders are reluctant, for a variety of reasons, to hold meetings with their staffs. But it's like dieting and exercise; the more you do it, the easier it becomes. This type of meeting will pay rich dividends in staff personal and professional growth and in improved communication. The sense of participation that can be gained by the effective use of staff meetings can lead to high morale and effective staff performance. As you begin to see the results of a cohesive staff functioning together well, you will realize the routine staff meeting is a management tool that should not be overlooked or underused. PMID:6917733

  20. Quantum Physics for Scientists and Technologists Fundamental Principles and Applications for Biologists, Chemists, Computer Scientists, and Nanotechnologists

    CERN Document Server

    Sanghera, Paul

    2011-01-01

    Presenting quantum physics for the non-physicists, Quantum Physics for Scientists and Technologists is a self-contained, cohesive, concise, yet comprehensive, story of quantum physics from the fields of science and technology, including computer science, biology, chemistry, and nanotechnology. The authors explain the concepts and phenomena in a practical fashion with only a minimum amount of math. Examples from, and references to, computer science, biology, chemistry, and nanotechnology throughout the book make the material accessible to biologists, chemists, computer scientists, and non-techn

  1. Extraction of High Quality RNA from Cannabis sativa Bast Fibres: A Vademecum for Molecular Biologists

    OpenAIRE

    Gea Guerriero; Lauralie Mangeot-Peter; Jean-Francois Hausman; Sylvain Legay

    2016-01-01

    In plants there is no universal protocol for RNA extraction, since optimizations are required depending on the species, tissues and developmental stages. Some plants/tissues are rich in secondary metabolites or synthesize thick cell walls, which hinder an efficient RNA extraction. One such example is bast fibres, long extraxylary cells characterized by a thick cellulosic cell wall. Given the economic importance of bast fibres, which are used in the textile sector, as well as in biocomposites ...

  2. L’utilité de la théorie du don The utility of gift theory Utilidad de la teoría del don

    Directory of Open Access Journals (Sweden)

    Norbert Alter

    2012-12-01

    Full Text Available Norbert Alter explique l’utilité et l’actualité de la théorie de Marcel Mauss pour penser la coopération en entreprise et l’engagement au travail. Il revient sur ses qualités heuristiques et sa portée critique nécessaires à mobiliser pour donner des clefs de compréhension et d’action au monde du travail. À cette contribution répond celle d’Alain Caillé, « Le paradigme du don face aux nouvelles réalités du monde du travail. Quelques remarques »Norbert Alter explains here the utility and topicality of Marcel Mauss’ theory in an analysis touching both on cooperation within a business structure and on people’s commitment to work. He reviews the heuristic qualities and critical scope that must be mobilised to create ways of understanding and acting within the work world. Alain Caillé responds to this contribution with his own article entitled, “ The gift paradigm facing new realities in the work world. Comments” (Le paradigme du don face aux nouvelles réalités du monde du travail. Quelques remarques.Norbert Alter explica la utilidad y la actualidad de la teoría de Marcel Mauss para pensar la cooperación en la empresa y el compromiso en el trabajo. Desde una nueva perspectiva, aborda las cualidades heurísticas de esa teoría y su alcance crítico, que es necesario movilizar para contar con claves de comprensión y de acción en el mundo laboral. A su contribución, Alain Caillé responde con el artículo “El paradigma del don frente a las nuevas realidades del mundo laboral. Algunas observaciones”.

  3. Women in cell biology: a seat at the table and a place at the podium.

    Science.gov (United States)

    Masur, Sandra Kazahn

    2013-01-01

    The Women in Cell Biology (WICB) committee of the American Society for Cell Biology (ASCB) was started in the 1970s in response to the documented underrepresentation of women in academia in general and cell biology in particular. By coincidence or causal relationship, I am happy to say that since WICB became a standing ASCB committee, women have been well represented in ASCB's leadership and as symposium speakers at the annual meeting. However, the need to provide opportunities and information useful to women in developing their careers in cell biology is still vital, given the continuing bias women face in the larger scientific arena. With its emphasis on mentoring, many of WICB's activities benefit the development of both men and women cell biologists. The WICB "Career Column" in the monthly ASCB Newsletter is a source of accessible wisdom. At the annual ASCB meeting, WICB organizes the career discussion and mentoring roundtables, childcare awards, Mentoring Theater, career-related panel and workshop, and career recognition awards. Finally, the WICB Speaker Referral Service provides a list of outstanding women whom organizers of scientific meetings, scientific review panels, and university symposia/lecture series can reach out to when facing the proverbial dilemma, "I just don't know any women who are experts."

  4. Empirical investigation of the ethical reasoning of physicians and molecular biologists – the importance of the four principles of biomedical ethics

    Directory of Open Access Journals (Sweden)

    Ebbesen Mette

    2007-10-01

    Full Text Available Abstract Background This study presents an empirical investigation of the ethical reasoning and ethical issues at stake in the daily work of physicians and molecular biologists in Denmark. The aim of this study was to test empirically whether there is a difference in ethical considerations and principles between Danish physicians and Danish molecular biologists, and whether the bioethical principles of the American bioethicists Tom L. Beauchamp and James F. Childress are applicable to these groups. Method This study is based on 12 semi-structured interviews with three groups of respondents: a group of oncology physicians working in a clinic at a public hospital and two groups of molecular biologists conducting basic research, one group employed at a public university and the other in a private biopharmaceutical company. Results In this sample, the authors found that oncology physicians and molecular biologists employed in a private biopharmaceutical company have the specific principle of beneficence in mind in their daily work. Both groups are motivated to help sick patients. According to the study, molecular biologists explicitly consider nonmaleficence in relation to the environment, the researchers' own health, and animal models; and only implicitly in relation to patients or human subjects. In contrast, considerations of nonmaleficence by oncology physicians relate to patients or human subjects. Physicians and molecular biologists both consider the principle of respect for autonomy as a negative obligation in the sense that informed consent of patients should be respected. However, in contrast to molecular biologists, physicians experience the principle of respect for autonomy as a positive obligation as the physician, in dialogue with the patient, offers a medical prognosis based upon the patients wishes and ideas, mutual understanding, and respect. Finally, this study discloses utilitarian characteristics in the overall conception of

  5. [Spanish neuroscience in times of Don Quixote].

    Science.gov (United States)

    Martín-Araguz, Antonio; Mikola, Yvett; Almendral-Doncel, Raquel; Campos-Bueno, Javier

    2016-02-16

    Introduccion. Miguel de Cervantes Saavedra publico su inmortal obra Don Quijote de la Mancha en plena epoca de crisis y decadencia en la España de la transicion entre los siglos XVI y XVII. Objetivo. En 2016 se conmemora el cuarto centenario del fallecimiento de nuestro insigne literato, por cuyo motivo analizamos en este articulo el estado de la neurociencia hispana, tanto en el texto quijotesco como en la obra de los autores coetaneos mas relevantes. Desarrollo. A pesar de las peyorativas circunstancias historicas, en la epoca de la transicion del Renacimiento al Barroco se produjo en la Corona de Castilla un florecimiento de la literatura (Siglo de Oro español) y de otras artes hispanas (pintura, escultura, arquitectura y musica), asi como una prodigiosa creatividad en el campo de la neurociencia, incluida en la filosofia natural. El medico Gomez Pereira, en su libro Antoniana Margarita, se adelanto en decadas a las bases del mecanicismo cerebral y del concepto de reflejo condicionado. El boticario Miguel Sabuco, en su texto Nueva filosofia, anticipo en siglos el concepto de neurotransmision. El medico Juan Huarte de San Juan fue el fundador de la neuropsicologia y la psicologia experimental; su texto Examen de ingenios ha sido uno de los textos cientificos mas influyentes y traducidos de todos los tiempos; sus conceptos quedan patentemente reflejados en el texto cervantino. Conclusion. Este analisis de la obra cervantina dentro del entorno cultural de la obra pretende ser un homenaje a la inmortal figura de nuestro 'Principe de los ingenios' en el cuarto centenario de su fallecimiento.

  6. Don Quixote e a transgressão do saber

    Directory of Open Access Journals (Sweden)

    Paulo Roberto Ceccarelli

    2009-01-01

    truth will constantly be replaced to make place to another one that, in turn, will also be replaced. Cervantes belongs to the intellectual tradition of Erasmus de Rotterdam, and his Don Quixote rambles thought the universe of Erasmus in which every truth is suspected, and everything bathes in incertitude. Don Quixote de La Mancha is the result of the wisdom of Rotterdam and craziness of La Mancha. By transgressing the "known", Don Quixote transgresses the truth that up to then was taken for sacred and as a guarantee of knowledge and, therefore, of a possible means of controlling the world. Transgression produces turning points but there is not such a thing like a "final transgressing", which would be the equivalent of recreating the myth of an absolute knowledge. According to author, both our theoretical and clinical practices lack the necessary transgressing movement that would force us to abandon the shelter of constructed truths.

  7. Le don, histoire du concept, évolution des pratiques

    Directory of Open Access Journals (Sweden)

    François Athané

    2009-09-01

    Full Text Available Le premier chapitre de cette thèse étudie la notion de don établie en anthropologie et en sociologie par l’Essai sur le don de Marcel Mauss . Il a semblé utile d’examiner en détail les faits sociaux et culturels dont parle Mauss : le potlatch, cette fameuse « guerre des cadeaux » des Amérindiens de la côte nord-ouest du Canada, le kula des archipels Trobriand, à l’est de la Nouvelle-Guinée, le hau des Maoris de Nouvelle-Zélande. La thèse centrale de l’anthropologie de Mauss est que dons et ca...

  8. Antidiabetic and antioxidant properties of alkaloids from Catharanthus roseus (L.) G. Don.

    Science.gov (United States)

    Tiong, Soon Huat; Looi, Chung Yeng; Hazni, Hazrina; Arya, Aditya; Paydar, Mohammadjavad; Wong, Won Fen; Cheah, Shiau-Chuen; Mustafa, Mohd Rais; Awang, Khalijah

    2013-01-01

    Catharanthus roseus (L.) G. Don is a herbal plant traditionally used by local populations in India, South Africa, China and Malaysia to treat diabetes. The present study reports the in vitro antioxidant and antidiabetic activities of the major alkaloids isolated from Catharanthus roseus (L.) G. Don leaves extract. Four alkaloids--vindoline I, vindolidine II, vindolicine III and vindolinine IV--were isolated and identified from the dichloromethane extract (DE) of this plant's leaves. DE and compounds I-III were not cytotoxic towards pancreatic β-TC6 cells at the highest dosage tested (25.0 µg/mL). All four alkaloids induced relatively high glucose uptake in pancreatic β-TC6 or myoblast C2C12 cells, with III showing the highest activity. In addition, compounds II-IV demonstrated good protein tyrosine phosphatase-1B (PTP-1B) inhibition activity, implying their therapeutic potential against type 2 diabetes. III showed the highest antioxidant potential in ORAC and DPPH assays and it also alleviated H₂O₂-induced oxidative damage in β-TC6 cells at 12.5 µg/mL and 25.0 µg/mL. PMID:23955322

  9. Antidiabetic and Antioxidant Properties of Alkaloids from Catharanthus roseus (L. G. Don

    Directory of Open Access Journals (Sweden)

    Won Fen Wong

    2013-08-01

    Full Text Available Catharanthus roseus (L. G. Don is a herbal plant traditionally used by local populations in India, South Africa, China and Malaysia to treat diabetes. The present study reports the in vitro antioxidant and antidiabetic activities of the major alkaloids isolated from Catharanthus roseus (L. G. Don leaves extract. Four alkaloids—vindoline I, vindolidine II, vindolicine III and vindolinine IV—were isolated and identified from the dichloromethane extract (DE of this plant’s leaves. DE and compounds I–III were not cytotoxic towards pancreatic β-TC6 cells at the highest dosage tested (25.0 µg/mL. All four alkaloids induced relatively high glucose uptake in pancreatic β-TC6 or myoblast C2C12 cells, with III showing the highest activity. In addition, compounds II–IV demonstrated good protein tyrosine phosphatase-1B (PTP-1B inhibition activity, implying their therapeutic potential against type 2 diabetes. III showed the highest antioxidant potential in ORAC and DPPH assays and it also alleviated H2O2-induced oxidative damage in β-TC6 cells at 12.5 µg/mL and 25.0 µg/mL.

  10. Mars and the Mind of Don

    Science.gov (United States)

    Solomon, S. C.

    2002-12-01

    Among the many interests of Don Turcotte have been the terrestrial planets, both as individual bodies and as test beds for generalizations on the processes that have affected the form, evolution, and interior dynamics of Earth-like planets. Over a span of more than 20 years, Turcotte and his collaborators have contributed many insights into the surface evolution and mantle dynamics of Mars on topics ranging from the nature of support of long-wavelength topography, the origin of the Tharsis rise, the internal thermal evolution and its effect on topography and lithospheric stress, and the history of outgassing. A summary of recent findings concerning early Martian evolution permits Turcotte's contributions to be placed within the context of current understanding. Core-mantle differentiation occurred within 10-15 My of the start of solar system evolution, on the basis of isotope systematics in Martian meteorites. A magnetic dynamo was active in the early Noachian but apparently ceased before the end of heavy bombardment. Crustal formation was largely complete by the earliest Noachian, on the basis of the density of both well-preserved and largely buried impact structures. Gravity-topography admittances for Noachian terrain suggests that the general south-to-north thinning of the crust may have been the result of lower crustal flow, but rapid cooling of the crust is indicated by the preservation of early Noachian relief and large crustal magnetization anomalies. The onset of the Tharsis province as a site of voluminous magmatism and concentrated deformation likely postdated the establishment of the crustal thickness dichotomy, but much of the magmatism occurred prior to the formation of late Noachian valley networks. Extensive resurfacing of the northern hemisphere occurred in the Noachian, on the basis of the detection of large impact structures superposed on older filled basins. Extensive evidence for water-surface interactions during this period supports the view

  11. Don Bosco Technical School: A Situational Survey and Strategic Analysis

    OpenAIRE

    Ravasco, Gerard

    2005-01-01

    Don Bosco Technical School in Phnom Penh is a typical example of a long-running non-profit institution in Cambodia. In this part of the world it is plainly called an NGO – a non-governmental organization. It provides vocational skills training education to some four hundred out-of-school youth every year. And yet it does not charge for tuition; nay more it even provides free lunch at noon. So how does Don Bosco Technical School survive this monetary based and profit oriented society? What are...

  12. Pia Petersen sous le signe de don Quichotte

    Directory of Open Access Journals (Sweden)

    Esther Bautista Naranjo

    2013-04-01

    Full Text Available This interview has been motivated by the Cervantean intertextuality of Pia Petersen’s (a French-speaking Danish author last novel, Le Chien de don Quichotte (2012. Departing from the values that the writer attributes to Cervantes’ work, I try to establish a connection with a specific critical trend in order to test, later on, to which extent this approach is used by the author to create a series of characters placed under the sign of don Quixote. Finally, I evaluate her novel’s relation with contemporary reality.

  13. Cross-reactivity features of deoxynivalenol (DON)-targeted immunoaffinity columns aiming to achieve simultaneous analysis of DON and major conjugates in cereal samples

    Science.gov (United States)

    Gonçalves, Carlos; Stroka, Joerg

    2016-01-01

    ABSTRACT Immunoaffinity columns (IACs) are a well-established tool in the determination of regulated mycotoxins in food and feed commodities. However, they also have the potential to become attractive pre-concentration and clean-up materials for the determination of masked (also called modified) mycotoxins, which have been recognised as important contributors to the toxicological hazard deriving from fungal spoilage of goods. However, the information available in the literature concerning the cross-reactivity of DON-IACs against the major conjugates (DON-3-G, 15-AcDON and 3-AcDON) is incomplete and often contradictory. We have carried out a detailed characterisation of the cross-reactivity of the four main IACs brands against DON and its conjugates as well as an assessment of the competition among the analytes. Only one IAC enabled the simultaneous analysis of all relevant DON forms while two missed 15-AcDON and the fourth one missed DON-3-G and 3-AcDON. In the case of the multivalent IAC, the analytes modified at the C-3 position compete for the antibody binding with preference for 3-AcDON (less spatially hindered) while DON-3-G has the more-hindered access to the active sites. Taking into consideration the levels of DON conjugates existing in real samples, the cross-reactivity of one DON-IAC allows a quantitative analysis of all of these analytes. Important but rather neglected aspects such as the continuous supply of IACs with identical characteristics, and of columns which are strictly blank, are also addressed in this paper. PMID:27243664

  14. Cross-reactivity features of deoxynivalenol (DON)-targeted immunoaffinity columns aiming to achieve simultaneous analysis of DON and major conjugates in cereal samples.

    Science.gov (United States)

    Gonçalves, Carlos; Stroka, Joerg

    2016-06-01

    Immunoaffinity columns (IACs) are a well-established tool in the determination of regulated mycotoxins in food and feed commodities. However, they also have the potential to become attractive pre-concentration and clean-up materials for the determination of masked (also called modified) mycotoxins, which have been recognised as important contributors to the toxicological hazard deriving from fungal spoilage of goods. However, the information available in the literature concerning the cross-reactivity of DON-IACs against the major conjugates (DON-3-G, 15-AcDON and 3-AcDON) is incomplete and often contradictory. We have carried out a detailed characterisation of the cross-reactivity of the four main IACs brands against DON and its conjugates as well as an assessment of the competition among the analytes. Only one IAC enabled the simultaneous analysis of all relevant DON forms while two missed 15-AcDON and the fourth one missed DON-3-G and 3-AcDON. In the case of the multivalent IAC, the analytes modified at the C-3 position compete for the antibody binding with preference for 3-AcDON (less spatially hindered) while DON-3-G has the more-hindered access to the active sites. Taking into consideration the levels of DON conjugates existing in real samples, the cross-reactivity of one DON-IAC allows a quantitative analysis of all of these analytes. Important but rather neglected aspects such as the continuous supply of IACs with identical characteristics, and of columns which are strictly blank, are also addressed in this paper. PMID:27243664

  15. Neutrons for biologists: a beginner's guide, or why you should consider using neutrons

    OpenAIRE

    Lakey, Jeremy H.

    2009-01-01

    From the structures of isolated protein complexes to the molecular dynamics of whole cells, neutron methods can achieve a resolution in complex systems that is inaccessible to other techniques. Biology is fortunate in that it is rich in water and hydrogen, and this allows us to exploit the differential sensitivity of neutrons to this element and its major isotope, deuterium. Furthermore, neutrons exhibit wave properties that allow us to use them in similar ways to light, X-rays and electrons....

  16. Extraction of High Quality RNA from Cannabis sativa Bast Fibres: A Vademecum for Molecular Biologists

    Directory of Open Access Journals (Sweden)

    Gea Guerriero

    2016-07-01

    Full Text Available In plants there is no universal protocol for RNA extraction, since optimizations are required depending on the species, tissues and developmental stages. Some plants/tissues are rich in secondary metabolites or synthesize thick cell walls, which hinder an efficient RNA extraction. One such example is bast fibres, long extraxylary cells characterized by a thick cellulosic cell wall. Given the economic importance of bast fibres, which are used in the textile sector, as well as in biocomposites as green substitutes of glass fibres, it is desirable to better understand their development from a molecular point of view. This knowledge favours the development of biotechnological strategies aimed at improving specific properties of bast fibres. To be able to perform high-throughput analyses, such as, for instance, transcriptomics of bast fibres, RNA extraction is a crucial and limiting step. We here detail a protocol enabling the rapid extraction of high quality RNA from the bast fibres of textile hemp, Cannabis sativa L., a multi-purpose fibre crop standing in the spotlight of research.

  17. U.S. NAVY OFFICER ATTITUDES ON THE REPEAL OF "DON'T ASK, DON'T TELL"

    OpenAIRE

    Appleman, Ryan P.; McLaughlin, Peter S.

    2013-01-01

    See a presentation from this author for this work: http://hdl.handle.net/10945/39528 "Don't Ask, Don't Tell" (DADT) prohibited gays from serving openly in the military from December 1993 to September 2011. In February 1994, a survey of Navy officers was administered at the Naval Postgraduate School exploring attitudes toward DADT. This survey was re-administered in 1996, 1999, 2004, and 2010. The surveys revealed an increasing acceptance of gays in the Navy. The present study, conducted po...

  18. CAS Biologists in Shanghai Step up Cooperation with Their West European Peers

    Institute of Scientific and Technical Information of China (English)

    Zhang Qin

    2003-01-01

    @@ Ⅰ. International Cooperation: A Booster for Developing Highlevel Research Bodies The Shanghai Institutes for Biological Sciences (SIBS), a CAS research arm in biology, consists of the Institute Biochemistry and Cell Biology, the Institute of Neuroscience, the Institute of Materia Medica, the Institute of Physiology and Ecology, and the Institute for Nutritional Sciences. In response to the current trends of S&T globalization and the interdisciplinary development of the life sciences, it deems international cooperation amajor component of its effort to uplift the overall capacity of knowledge creation at SIBS.

  19. IDEA: An Interdisciplinary Unit Comparing "Don Quixote" to "Hamlet."

    Science.gov (United States)

    Harris, Mary J. G.

    2001-01-01

    Describes an idea for teaching language through content-based instruction in which a high school Spanish class studying a shortened abridged version of Cervantes'"Don Quixote" and an English class reading Shakespeare's "Hamlet," did a simple comparative analysis of the two texts. (Author/VWL)

  20. A phenolic glycoside from Fagopyrum dibotrys (D. Don) Hara

    Institute of Scientific and Technical Information of China (English)

    Zheng Zhong Bai; Xiao Hui Zhang; Li Jiang Xuan; Feng Kui Mo

    2007-01-01

    A new phenolic constituent along with five known compounds were isolated from Fagopyrum dibotrys (D.Don) Hara. The new compound was characterized as 1,3-dimethoxy-2-O-β-xylo-pyranosyl-5-O-β-glucopyranosyl-benzene, by spectroscopic analysis and enzymatic hydrolysis.

  1. Fjodor Bondartshuk dodelal "Tihhi Don" ottsa / Olga Saburova

    Index Scriptorium Estoniae

    Saburova, Olga

    2006-01-01

    Fjodor Bondartshuk monteeris kokku tema isa Sergei poolt Itaalias 13 aastat tagasi pooleli jäänud Mihhail Sholohhovi romaani järgi tehtud teleseriaali "Vaikne Don", mida hakkab Venemaa televisiooni 1. Kanal näitama 7. novembril

  2. "Tihhi Don" : Fjodor Bondartshuk zakontshil film svojego otsa / Darja Zavgorodnjaja

    Index Scriptorium Estoniae

    Zavgorodnjaja, Darja

    2006-01-01

    Fjodor Bondartshuk monteeris kokku tema isa Sergei poolt Itaalias 13 aastat tagasi pooleli jäänud Mihhail Sholohhovi romaani järgi tehtud teleseriaali "Vaikne Don", mida hakkab Venemaa televisiooni 1. Kanal näitama 7. novembril

  3. "Don Kihotõ" nazõvajut lutshshih / Boris Tuch

    Index Scriptorium Estoniae

    Tuch, Boris, 1946-

    2003-01-01

    Lõppenud festivali FIPRESCI auhinna sai türgi film "Kauge" ("Uzak") : režissöör Nuri Bilge Ceylan. Rahvusvaheline filmiklubide föderatsioon (FICC) andis oma auhinna Don Quijote Andrei Zvjagintsevi filmile "Tagasitulek" ("Vozvrashtshenije") ja eriauhinna hiinlase Chen Kaige filmile "Üheskoos" ("Han ni zai ylki")

  4. Reverse enGENEering of Regulatory Networks from Big Data: A Roadmap for Biologists.

    Science.gov (United States)

    Dong, Xiaoxi; Yambartsev, Anatoly; Ramsey, Stephen A; Thomas, Lina D; Shulzhenko, Natalia; Morgun, Andrey

    2015-01-01

    Omics technologies enable unbiased investigation of biological systems through massively parallel sequence acquisition or molecular measurements, bringing the life sciences into the era of Big Data. A central challenge posed by such omics datasets is how to transform these data into biological knowledge, for example, how to use these data to answer questions such as: Which functional pathways are involved in cell differentiation? Which genes should we target to stop cancer? Network analysis is a powerful and general approach to solve this problem consisting of two fundamental stages, network reconstruction, and network interrogation. Here we provide an overview of network analysis including a step-by-step guide on how to perform and use this approach to investigate a biological question. In this guide, we also include the software packages that we and others employ for each of the steps of a network analysis workflow. PMID:25983554

  5. L’utilité de la théorie du don The utility of gift theory Utilidad de la teoría del don

    OpenAIRE

    Norbert Alter

    2012-01-01

    Norbert Alter explique l’utilité et l’actualité de la théorie de Marcel Mauss pour penser la coopération en entreprise et l’engagement au travail. Il revient sur ses qualités heuristiques et sa portée critique nécessaires à mobiliser pour donner des clefs de compréhension et d’action au monde du travail. À cette contribution répond celle d’Alain Caillé, « Le paradigme du don face aux nouvelles réalités du monde du travail. Quelques remarques »Norbert Alter explains here the utility and topica...

  6. Viajando con Don Quijote en el siglo XXI

    Directory of Open Access Journals (Sweden)

    Odilíe Rojas López

    2006-06-01

    Full Text Available

    Resumen. El Quijote ha cabalgado con nosotros por largo tiempo y como si no fuera suficiente la lucha contra los molinos de viento, don Quijote de la Mancha tendrá que luchar contra muchas situaciones de nuestra época actual. ¿Logrará esta hazaña el Caballero de la Triste Figura? El reto es dar la respuesta.

    Por tal motivo, la autora de este ensayo traslada al Caballero de la Triste Figura y lo inserta a la sociedad del siglo XXI. Transforma los valores, costumbres y puntos de vistas de la época en que se desarrolla la obra literaria y establece una serie de correlaciones entre el texto literario, el personaje principal y las diversas situaciones cotidianas actuales. Ciertamente, Don Quijote seguirá luchando contra las situaciones de nuestra época actual. Sin duda alguna este hidalgo está aún entre nosotros.

     

    Abstract. Don Quixote has ridden with us for a long time now, and as if his fight against windmills were not enough, Don Quixote still has many more difficult situations to combat in the present times.  Will the Knight of the Sad Figure accomplish this new deed?

    It is our challenge to find an answer to this inquiry. For this purpose, the author of this essay inserts the Knight of the Sad Figure in XXI century society. The author transforms the values, costumes and points of view of the times of the literary piece and establishes a series of relationships between the literary text, the main character and several current daily life situations. Don Quixote will certainly continue his fight against all the conditions we encounter nowadays. There is no doubt, then, that this knight still lives among us.

  7. Microfluidics made easy: A robust low-cost constant pressure flow controller for engineers and cell biologists.

    Science.gov (United States)

    Mavrogiannis, Nicholas; Ibo, Markela; Fu, Xiaotong; Crivellari, Francesca; Gagnon, Zachary

    2016-05-01

    Over the last decade, microfluidics has become increasingly popular in biology and bioengineering. While lab-on-a-chip fabrication costs have continued to decrease, the hardware required for delivering controllable fluid flows to the microfluidic devices themselves remains expensive and often cost prohibitive for researchers interested in starting a microfluidics project. Typically, microfluidic experiments require precise and tunable flow rates from a system that is simple to operate. While many labs use commercial platforms or syringe pumps, these solutions can cost thousands of dollars and can be cost prohibitive. Here, we present an inexpensive and easy-to-use constant pressure system for delivering flows to microfluidic devices. The controller costs less than half the price of a single syringe pump but can independently switch and deliver fluid through up to four separate fluidic inlets at known flow rates with significantly faster fluid response times. It is constructed of readily available pressure regulators, gauges, plastic connectors and adapters, and tubing. Flow rate is easily predicted and calibrated using hydraulic circuit analysis and capillary tubing resistors. Finally, we demonstrate the capabilities of the flow system by performing well-known microfluidic experiments for chemical gradient generation and emulsion droplet production. PMID:27279931

  8. Biologists Bridging Science and the Conservation Movement : The Rise of Nature Conservation and Nature Management in the Netherlands, 1850-1950

    NARCIS (Netherlands)

    van der Windt, Henny J.

    2012-01-01

    This paper investigates the importance and various roles of amateur naturalists and biologists in the conservation movement between 1850 and 1950, in particular in the Netherlands. It is concluded that biological sciences were important resources for Dutch nature conservation, although the extent of

  9. CDC Vital Signs–Blood Pressure Medicines Don’t Work If People Don’t Take Them

    Centers for Disease Control (CDC) Podcasts

    2016-09-13

    Blood pressure medicines don’t work if people don’t take them. Learn how health care systems can work with patients to make taking medicines easier.  Created: 9/13/2016 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 9/13/2016.

  10. Polarized Light Experiment, Presa Don Martin, Coahuila, Mexico

    Science.gov (United States)

    1989-01-01

    This is a single scene from a pair (frames 021 and 024) to study the effects of polarized light in Earth Observations. One scene was exposed with vertically polarized light, the other, horizontally. The subject in this study, is a lake behind Presa (dam) Don Martin (27.5N, 100.5W) on the edge of the Rio Grande Plain near it's boundry with the Sierra Madre Orientral in Coahuila, Mexico.

  11. Deoxynivalenol (DON) degradation and peroxidase enzyme activity in submerged fermentation

    OpenAIRE

    Jaqueline Garda-Buffon; Larine Kupski; Eliana Badiale-Furlong

    2011-01-01

    This work aims to evaluate deoxynivalenol degradation by Aspergillus oryzae and Rhizopus oryzae in a submerged fermentation system and to correlate it to the activity of oxydo-reductase enzymes. The submerged medium consisted of sterile distilled water contaminated with 50 μg of DON and 4 × 10(6) spore.mL-1 inoculum of Aspergillus oryzae and Rhizopus oryzae species, respectively in each experiment. Sampling was performed every 24 hours for monitoring the peroxidase specific activity, and ever...

  12. STATE EVALUATION OF WOODY PLANTS IN ROSTOV-ON-DON

    OpenAIRE

    Gudzenko E. O.

    2015-01-01

    There has been conducted the analysis of woody plants quantity in various types of stands in Rostov-on-Don, as well as the evaluation of their state and the rate of environmental resistance destabilization. It has been ascertained that the stands of almost all of the urban gardening objects are in a salvaged condition. There have been elaborated recommendations for enhancement of the stands inspected

  13. STATE EVALUATION OF WOODY PLANTS IN ROSTOV-ON-DON

    Directory of Open Access Journals (Sweden)

    Gudzenko E. O.

    2015-10-01

    Full Text Available There has been conducted the analysis of woody plants quantity in various types of stands in Rostov-on-Don, as well as the evaluation of their state and the rate of environmental resistance destabilization. It has been ascertained that the stands of almost all of the urban gardening objects are in a salvaged condition. There have been elaborated recommendations for enhancement of the stands inspected

  14. Pharmacognostical studies of leaves of Combretum albidum G. Don

    OpenAIRE

    Zalke, Ashish S.; Duraiswamy, B.; Gandagule, Upendra B.

    2013-01-01

    Background: Combretum albidum Don belonging to family Combretaceae is an unexplored medicinal plant in the Indian medicinal system. According to ethnobotanical information, the leaves are used in the treatment of peptic ulcer and its fruits are used in diarrhoea and dysentery. Stem bark is used in the treatment of jaundice and skin diseases. The problem encountered in standardisation of this medicinal plant is its identification by source. Materials and Methods: The pharmacognostical stud...

  15. Don Quixote og romangenren

    DEFF Research Database (Denmark)

    Kluge, Sofie

    Through the centuries Cervantes' Don Quixote (1605/1615) has delighted readers, inspired artists, and helped form our perception of early modern Spain. Moreover, it has played a major part in the development and theoretisation of one of the modern world's most central artistic forms: the novel. D...... as introductions to some of the great figures of novelistic theory, including Friedrich Schlegel, Viktor Šklovskij, Georg Lukács, and Milan Kundera....

  16. Catharanthus roseus (L.) G. Don - plant regeneration and alkaloids content

    OpenAIRE

    Mirosława Furmanowa; Hanna Olędzka; Joanna Józefowicz; Agnieszka Pietrosiuk

    2014-01-01

    We describe here a regeneration of plantlets of Catharanthus roseus (L.) G. Don from shoot tips and axillary buds. Shoot tips were excised from 7-day-old seedlings and were incubated in solid Nitsch and Nitsch (NN) medium supplemented with kinetin, benzyladenine (BA), indole-3-butyric acid (IBA) and β-indolylacetic acid (IAA) in various combinations. After two months in culture, regenerated rooted plantlets were cut and transferred to a new medium; the explants contained shoot tips or axillar...

  17. Épistémologie de la biologie et conceptualisation du vivant chez des futurs enseignants et biologistes

    Directory of Open Access Journals (Sweden)

    Simard Catherine

    2015-01-01

    Full Text Available L’ensemble des résultats présentés dans cet article relève d’une étude doctorale qui s’est intéressée à l’histoire de la biologie et aux grandes conceptions qui ont alimenté la construction des savoirs en biologie jusqu’à présent (Simard, 2015 [1]. À cet égard, un ensemble de conceptions du vivant a été sélectionné et circonscrit afin de développer un nouvel outil d’évaluation estimant la présence de l’une ou l’autre de ces conceptions chez les futurs enseignants et biologistes. Pour certaines conceptions s’avérant désuètes, la problématique est qu’elles se voient reconduites, encore aujourd’hui, dans la pensée scientifique. C’est alors en termes d’obstacle épistémologique historique à une conceptualisation contemporaine du vivant qu’elles sont appréhendées. Cette caractérisation de la conceptualisation du vivant et l’identification de conceptionsobstacles sont un passage nécessaire afin d’étudier, ultérieurement, les processus d’apprentissage et l’élaboration de séquences didactiques en les considérant au profit d’une compréhension actuelle du vivant. Enfin, est présentée brièvement l’influence positive que peut avoir une formation scientifique en biologie sur la conceptualisation du vivant et sur les conceptions-obstacles, qui se voient alors décliner*.

  18. Environmental radiation effects. A need to question old paradigms and to enhance collaboration between radiation biologists and radiation ecologists

    International Nuclear Information System (INIS)

    The radiological sciences are a real enigma- the maturity and depth of understanding concerning human dosimetry contrasts sharply with our shallow understanding about radiological effects to biota. The richness of the radiological sciences is apparent by looking at the refinements made to the fundamental unit used in human dosimetry. The radiological sciences have developed to where probabilistic risk factors can now be applied that predict specific deleterious effects to humans per unit dose. And yet, these same radiological sciences that have made such advances in human dosimetry, are primitive when effects to biota are concerned. There are no specialized units, no agreed upon weighting factors, no factors that account for distributions within an organism's body, and certainly no risk factors. There are no internationally agreed upon criteria or policies that explicitly address protection of the environment from ionizing radiation. There is not even agreement as to what endpoint should be measured to quantify an environmental effect. The bold aspect of the ICRP framework is the inclusion of sub-lethal effects (reduced reproductive success, scorable DNA damage) as endpoints. A major research consortium funded by the European Union, is also recommending that cytogenetic damage be used as an effect endpoint. The inclusion of sub-lethal endpoints begs for a linkage between molecular effects and those observed in individuals and populations. To do so, will require a strengthening of what has traditionally been separated disciplines of radiation biology and radiation ecology. The impacts of phenomena studied in the petri dishes of radiation biologists (such as genomic instability, adaptive response, and bystander effects) need to be explored and correlated to effects observed in whole organisms and populations, in collaboration with radiation ecologists. (M. Suetake)

  19. KESALAHAN PENGGUNAAN PUNGTUASI DALAM SURAT DINAS DI SD FRATER DON BOSCO TARAKAN

    OpenAIRE

    Yohanis Tandi

    2011-01-01

    YOHANIS TANDI , 2011. Kesalahan Penggunaan Pungtuasi dalam Surat Dinas di SD Frater Don Bosko Tarakan. Skipsi Program Studi Pendidikan Bahasa, Sastra Indonesia dan Daerah FKIP Universitas Borneo Tarakan, pembimbing: (1) Zulkifli, M.Pd. (II) Drs. Petrus Tenabean. Rumusan masalah dalam penelitian ini, yaitu penggunaan pungtuasi dalam surat dinas di SD Frater Don Bosko tarakan. Penelitian ini bertujuan untuk mendeskripsikan pemakaian pungtuasi di dalam surat dinas di SD Frarter Don Bosko Taraka...

  20. The Authors of the Ingenious Hidalgo Don Quixote of La Mancha

    OpenAIRE

    Branka Kalenić Ramšak

    2008-01-01

    Even today literary criticism still considers the novel Don Quixote the first modern European novel because it fundamentally changes both the concept of literary creation and the findings regarding literary reception. Miguel de Cervantes Saavedra published the first part of his chivalric novel in 1605 in Madrid with the title El ingenioso hidalgo don Quijote de la Mancha. He published the second part, titled El ingenioso caballero don Quijote de la Mancha, ten years later (i.e., in 1615), aga...

  1. L’utilité de la théorie du don

    OpenAIRE

    Alter, Norbert

    2012-01-01

    Norbert Alter explique l’utilité et l’actualité de la théorie de Marcel Mauss pour penser la coopération en entreprise et l’engagement au travail. Il revient sur ses qualités heuristiques et sa portée critique nécessaires à mobiliser pour donner des clefs de compréhension et d’action au monde du travail. À cette contribution répond celle d’Alain Caillé, « Le paradigme du don face aux nouvelles réalités du monde du travail. Quelques remarques »

  2. Don Quixote Pond: A Small Scale Model of Weathering and Salt Accumulation

    Science.gov (United States)

    Englert, P.; Bishop, J. L.; Patel, S. N.; Gibson, E. K.; Koeberl, C.

    2015-01-01

    The formation of Don Quixote Pond in the North Fork of Wright Valley, Antarctica, is a model for unique terrestrial calcium, chlorine, and sulfate weathering, accumulation, and distribution processes. The formation of Don Quixote Pond by simple shallow and deep groundwater contrasts more complex models for Don Juan Pond in the South Fork of Wright Valley. Our study intends to understand the formation of Don Quixote Pond as unique terrestrial processes and as a model for Ca, C1, and S weathering and distribution on Mars.

  3. La lectura unamuniana de Don Quijote de La Mancha

    OpenAIRE

    Stolet Correia, Cristiane Agnes

    2011-01-01

    El objetivo de la presente comunicación es pensar al personaje Don Quijote de la Mancha bajo la perspectiva de Miguel de Unamuno, a partir principalmente de sus textos Vida de Don Quijote y Sancho y El caballero de la triste figura, ambos en diálogo con el poema Letanía de Nuestro Señor Don Quijote, de Rubén Darío. De acuerdo con Unamuno, Don Quijote es el caballero de la fe, pero de la fe basada en la duda. Se pretende así rescatar el sentido de la fe quijotesca y profundizar la idea de la v...

  4. Don Quichotte, un don quichotte ? Déprogrammation d’un stéréotype

    Directory of Open Access Journals (Sweden)

    Marta Cuenca-Godbert

    2009-12-01

    Full Text Available En réaction à la publication de la suite apocryphe de Don Quichotte de la Manche, Cervantès utilise dans sa seconde partie les ressorts narratifs de la stéréotypie littéraire pour montrer à quel point son personnage diffère de ce qu'en a fait Alonso Fernández de Avellaneda, continuateur peu respectueux de sa personne et de son personnage. Le refus de voir en don Quichotte un simple stéréotype du chevalier errant chimérique est particulièrement manifeste dans la série d'aventures se déroulant au château des ducs, où don Quichotte, par sa renommée littéraire littéralement reconnue, est traité en chevalier par des personnages manipulateurs de situations, qui visent à produire chez lui des réactions programmées pour s’en amuser. Il apparaît alors que le simple agrégat d'ingrédients propres au stéréotype littéraire chevaleresque finit par ne pas produire l'issue escomptée.En la Segunda parte del Quijote, Cervantes reacciona ante la publicación de la de Alonso Fernández de Avellaneda, continuador poco respetuoso con la persona del autor primigenio y su personaje. Recurriendo a los procedimientos narrativos de la estereotipia literaria deja bien claro qué diferente resulta ser el personaje original respecto al del continuador. La negativa de ver a don Quijote como un mero estereotipo del caballero andante loco se pone particularmente de manifiesto en la serie de aventuras que transcurren en el palacio de los duques. Allí, los personajes reconocen literalmente la fama literaria de don Quijote. Generan, desde situaciones ficticias, tipos de tratamiento que se dispensarían a un verdadero caballero andante. Como resultado, se desencadena una serie de reacciones programadas con las que pretenden divertirse. Sin embargo, queda claro que la mera acumulación de ingredientes propios del estereotipo literario caballeresco no produce el resultado esperado.

  5. Mechanistic modeling confronts the complexity of molecular cell biology

    OpenAIRE

    Phair, Robert D.

    2014-01-01

    Mechanistic modeling has the potential to transform how cell biologists contend with the inescapable complexity of modern biology. I am a physiologist–electrical engineer–systems biologist who has been working at the level of cell biology for the past 24 years. This perspective aims 1) to convey why we build models, 2) to enumerate the major approaches to modeling and their philosophical differences, 3) to address some recurrent concerns raised by experimentalists, and then 4) to imagine a fu...

  6. Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae).

    Science.gov (United States)

    Tiong, Soon Huat; Looi, Chung Yeng; Arya, Aditya; Wong, Won Fen; Hazni, Hazrina; Mustafa, Mohd Rais; Awang, Khalijah

    2015-04-01

    Vindogentianine, a new indole alkaloid together with six known alkaloids, vindoline, vindolidine, vindolicine, vindolinine, perivine and serpentine were isolated from leaf extract (DA) of Catharanthus roseus (L.) G. Don. Their structures were elucidated by spectroscopic methods; NMR, MS, UV and IR. Vindogentianine is a dimer containing a vindoline moiety coupled to a gentianine moiety. After 24h incubation, vindogentianine exhibited no cytotoxic effect in C2C12 mouse myoblast and β-TC6 mouse pancreatic cells (IC50>50μg/mL). Real-time cell proliferation monitoring also indicated vindogentianine had little or no effect on C2C12 mouse myoblast cell growth at the highest dose tested (200μg/mL), without inducing cell death. Vindogentianine exhibited potential hypoglycemic activity in β-TC6 and C2C12 cells by inducing higher glucose uptake and significant in vitro PTP-1B inhibition. However, in vitro α-amylase and α-glucosidase inhibition assay showed low inhibition under treatment of vindogentianine. This suggests that hypoglycemic activity of vindogentianine may be due to the enhancement of glucose uptake and PTP-1B inhibition, implying its therapeutic potential against type 2 diabetes. PMID:25665941

  7. A focus on parietal cells as a renewing cell population

    Institute of Scientific and Technical Information of China (English)

    Sherif; M; Karam

    2010-01-01

    The fact that the acidsecreting parietal cells undergo continuous renewal has been ignored by many gastroenterologists and cell biologists. In the past, it was thought that these cells were static. However, by using 3Hthymidine radioautography in combination with electron microscopy, it was possible to demonstrate that parietal cells belong to a continuously renewing epithelial cell lineage. In the gastric glands, stem cells anchored in the isthmus region are responsible for the production of parietal cells...

  8. Entre burlas y veras: Don Quijote como loco-cuerdo

    OpenAIRE

    Gallardo Saborido, Emilio J.

    2009-01-01

    Este artículo indaga en la configuración plural y ambivalente de la figura de Don Quijote, centrándose específicamente en la caracterización de este personaje como loco-cuerdo. Así pues, se consideran varios mecanismos con los que Cervantes jugó para tal fin: desde el uso de la verosimilitud y la parodia, hasta el recurso a modelos médicos propios de su época, pasando por revisiones de arquetipos prestigiosos, como el del caballero erasmista. Las conclusiones obtenidas inciden en la contrapos...

  9. Comunidades de afectados, procomún y don expandido

    OpenAIRE

    Lafuente, Antonio; Corsín Jiménez, Alberto

    2010-01-01

    El don existe. Existe, pero como tantas cosas en nuestro mundo, incluso muy importantes, parece invisible. Allí siempre habrá una mujer cuidando de un bebé, un enfermo o un anciano. En los alrededores, cualquiera que sea la dirección a donde apuntemos, habrá alguien usando la lengua, respirando aire, jugando en la calle o protestando por el ruido. Y conste que podríamos subir el tono y mencionar todo lo que tenga que ver con añorar justicia, gozar paisajes, reclamar salud o disfrutar el si...

  10. Digitaalisen markkinointiviestinnän suunnittelu Facebookissa: Ravintola Don Corleone

    OpenAIRE

    Dondych, Fanny

    2016-01-01

    Tämän opinnäytetyön tavoitteena oli kehittää viestintäsuunnitelma ravintolalle Don Corleone digi-taalisesta markkinointiviestinnästä Facebookissa. Työn tarkoituksena oli tehostaa ravintolan mark-kinointiviestintää Facebookissa, mikä johtaisi asiakkaiden lisääntymiseen ja sitä kautta liiketoimin-nan kasvuun. Sosiaalisen median palveluista viestintäkanavaksi valittiin Facebook toimeksiantajan toiveesta sekä Facebookin suuren suosion ja soveltuvuuden vuoksi. Facebookissa on valmiiksi pal-jon eri...

  11. 78 FR 52172 - Don W. Gilbert Hydro Power, LLC; Notice of Availability of Environmental Assessment

    Science.gov (United States)

    2013-08-22

    ... Commission's (Commission) regulations, 18 CFR part 380 (Order No. 486, 52 FR 47897), the Office of Energy... Energy Regulatory Commission Don W. Gilbert Hydro Power, LLC; Notice of Availability of Environmental... applicant Don W. Gilbert Hydro Power, LLC certification water quality certification CFR Code of...

  12. The Generation of 1898 and Cervantes : The invention of Don Quixote as a national symbol

    NARCIS (Netherlands)

    Storm, H.J.; Achiri, N.; Baraibar, A.; Schmelzer, F.K.E.

    2015-01-01

    Don Quixote became a Spanish national symbol thanks to the authors the Generation of 1898, such as Miguel de Unamuno, Azorín and Francisco Navarra Ledesma, who published their new interpretations during the 1905 commemoration of the publication of Cervantes', The Ingenious Gentleman Don Quixote of L

  13. SAT-Based Complete Don't-Care Computation for Network Optimization

    CERN Document Server

    Mishchenko, Alan

    2011-01-01

    This paper describes an improved approach to Boolean network optimization using internal don't-cares. The improvements concern the type of don't-cares computed, their scope, and the computation method. Instead of the traditionally used compatible observability don't-cares (CODCs), we introduce and justify the use of complete don't-cares (CDC). To ensure the robustness of the don't-care computation for very large industrial networks, a optional windowing scheme is implemented that computes substantial subsets of the CDCs in reasonable time. Finally, we give a SAT-based don't-care computation algorithm that is more efficient than BDD-based algorithms. Experimental results confirm that these improvements work well in practice. Complete don't-cares allow for a reduction in the number of literals compared to the CODCs. Windowing guarantees robustness, even for very large benchmarks on which previous methods could not be applied. SAT reduces the runtime and enhances robustness, making don't-cares affordable for a v...

  14. An Interview with Don Meyer on Siblings of Individuals with Disabilities

    Science.gov (United States)

    Laman, Effie; Shaughnessy, Michael F.

    2007-01-01

    In this article, the authors present an interview with Don Meyer, an advocate for the siblings of children with disabilities. Don Meyer has conducted "SibShops," a peer support and information for school-age siblings of children with disabilities, and written extensively on the difficult situation of children in this underserved demographic. Here,…

  15. Basic statistics in cell biology.

    Science.gov (United States)

    Vaux, David L

    2014-01-01

    The physicist Ernest Rutherford said, "If your experiment needs statistics, you ought to have done a better experiment." Although this aphorism remains true for much of today's research in cell biology, a basic understanding of statistics can be useful to cell biologists to help in monitoring the conduct of their experiments, in interpreting the results, in presenting them in publications, and when critically evaluating research by others. However, training in statistics is often focused on the sophisticated needs of clinical researchers, psychologists, and epidemiologists, whose conclusions depend wholly on statistics, rather than the practical needs of cell biologists, whose experiments often provide evidence that is not statistical in nature. This review describes some of the basic statistical principles that may be of use to experimental biologists, but it does not cover the sophisticated statistics needed for papers that contain evidence of no other kind.

  16. Blood Pressure Medicines Don’t Work If People Don’t Take Them PSA (:60)

    Centers for Disease Control (CDC) Podcasts

    2016-09-13

    This 60 second public service announcement is based on the September 2016 CDC Vital Signs report. Blood pressure medicines don’t work if people don’t take them. Learn how health care systems can work with patients to make taking medicines easier.  Created: 9/13/2016 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 9/13/2016.

  17. Research Advancement on Biosynthesis and Biodegradation of Deoxynivalenol (DON)%呕吐毒素(DON)生物合成和降解研究进展

    Institute of Scientific and Technical Information of China (English)

    曹慧英; 伍松陵; 孙长坡

    2013-01-01

    Vomitoxin,also known as deoxynivalenol (DON),is a kind of secondary metabolites produced by certain Fusarium species;it is one of the common mycotoxins which polluted the food.With the frequent occurrence of climate disasters domestically,the trend of DON pollution exacerbates,so prevention and treatment of DON contamination are imminent.This peper covers the physical and chemical properties of DON,analysis of the DON biosynthetic pathways based on its chemical structure and genes,and summarizes biodegradation of DON by microorganism,speculates possible sites of degradation,degradation pathways and products.Based on a comprehensive understanding of DON,it aims to inspire to seek safe,efficient and cost effective biodegradation pathways using biotechnology,finally to ensure grain and food safety and protect health of consumers.%呕吐毒素(Vomitoxin),即脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON),是镰刀菌产生的次生代谢物质,是污染粮食的主要真菌毒素.随着我国灾害气候的频频发生,DON的污染程度呈现加剧趋势,因此DON污染的控制迫在眉睫.本文阐述了DON的物理化学特性,结合化学结构和基因遗传分析了DON的生物合成途径,并综述了微生物对DON的生物降解研究,推测了DON可能的作用位点、降解途径及降解产物,以此启发利用生物技术,探求安全、高效,低成本的DON生物降解途径,确保粮食和食品安全、保护消费者健康.

  18. Dual Effects Exerted in Vitro by Micromolar Concentrations of Deoxynivalenol on Undifferentiated Caco-2 Cells

    Directory of Open Access Journals (Sweden)

    Gina Manda

    2015-02-01

    Full Text Available Contamination of crops used for food and feed production with Fusarium mycotoxins, such as deoxynivalenol (DON, raise important health and economic issues all along the food chain. Acute exposure to high DON concentrations can alter the intestinal barrier, while chronic exposure to lower doses may exert more subtle effects on signal transduction pathways, leading to disturbances in cellular homeostasis. Using real-time cellular impedance measurements, we studied the effects exerted in vitro by low concentrations of DON (0.37–1.50 μM, relevant for mycotoxin-contaminated food, on the proliferation of undifferentiated Caco-2 cells presenting a tumorigenic phenotype. A 1.5 μM concentration of DON maintained cell adherence of non-proliferating Caco-2 cells, whilst arresting the growth of actively proliferating cells compared with control Caco-2 cells in vitro. At 0.37 μM, DON enhanced Caco-2 cell metabolism, thereby triggering a moderate increase in cell proliferation. The results of the current study suggested that low concentrations of DON commonly detected in food may either limit or sustain the proliferation of colon cancer cells, depending on their proliferation status and on DON concentration. Soluble factors released by Lactobacillus strains can partially counteract the inhibitory action of DON on actively proliferating colon cancer cells. The study also emphasized that real-time cellular impedance measurements were a valuable tool for investigating the dynamics of cellular responses to xenobiotics.

  19. Dual effects exerted in vitro by micromolar concentrations of deoxynivalenol on undifferentiated caco-2 cells.

    Science.gov (United States)

    Manda, Gina; Mocanu, Mihaela Andreea; Marin, Daniela Eliza; Taranu, Ionelia

    2015-02-01

    Contamination of crops used for food and feed production with Fusarium mycotoxins, such as deoxynivalenol (DON), raise important health and economic issues all along the food chain. Acute exposure to high DON concentrations can alter the intestinal barrier, while chronic exposure to lower doses may exert more subtle effects on signal transduction pathways, leading to disturbances in cellular homeostasis. Using real-time cellular impedance measurements, we studied the effects exerted in vitro by low concentrations of DON (0.37-1.50 μM), relevant for mycotoxin-contaminated food, on the proliferation of undifferentiated Caco-2 cells presenting a tumorigenic phenotype. A 1.5 μM concentration of DON maintained cell adherence of non-proliferating Caco-2 cells, whilst arresting the growth of actively proliferating cells compared with control Caco-2 cells in vitro. At 0.37 μM, DON enhanced Caco-2 cell metabolism, thereby triggering a moderate increase in cell proliferation. The results of the current study suggested that low concentrations of DON commonly detected in food may either limit or sustain the proliferation of colon cancer cells, depending on their proliferation status and on DON concentration. Soluble factors released by Lactobacillus strains can partially counteract the inhibitory action of DON on actively proliferating colon cancer cells. The study also emphasized that real-time cellular impedance measurements were a valuable tool for investigating the dynamics of cellular responses to xenobiotics.

  20. Dual Effects Exerted in Vitro by Micromolar Concentrations of Deoxynivalenol on Undifferentiated Caco-2 Cells

    Science.gov (United States)

    Manda, Gina; Mocanu, Mihaela Andreea; Marin, Daniela Eliza; Taranu, Ionelia

    2015-01-01

    Contamination of crops used for food and feed production with Fusarium mycotoxins, such as deoxynivalenol (DON), raise important health and economic issues all along the food chain. Acute exposure to high DON concentrations can alter the intestinal barrier, while chronic exposure to lower doses may exert more subtle effects on signal transduction pathways, leading to disturbances in cellular homeostasis. Using real-time cellular impedance measurements, we studied the effects exerted in vitro by low concentrations of DON (0.37–1.50 μM), relevant for mycotoxin-contaminated food, on the proliferation of undifferentiated Caco-2 cells presenting a tumorigenic phenotype. A 1.5 μM concentration of DON maintained cell adherence of non-proliferating Caco-2 cells, whilst arresting the growth of actively proliferating cells compared with control Caco-2 cells in vitro. At 0.37 μM, DON enhanced Caco-2 cell metabolism, thereby triggering a moderate increase in cell proliferation. The results of the current study suggested that low concentrations of DON commonly detected in food may either limit or sustain the proliferation of colon cancer cells, depending on their proliferation status and on DON concentration. Soluble factors released by Lactobacillus strains can partially counteract the inhibitory action of DON on actively proliferating colon cancer cells. The study also emphasized that real-time cellular impedance measurements were a valuable tool for investigating the dynamics of cellular responses to xenobiotics. PMID:25690693

  1. Vivisection au ralenti: Don DeLillo’s Point Omega

    Directory of Open Access Journals (Sweden)

    Ruxanda BONTILĂ

    2014-12-01

    Full Text Available Reading a Don DeLillo is like walking on glass, that is, you need hold your breath so that nothing could deter you from focusing on the mission, if you want to keep safe and mostly sound. The writer’s latest novel, Point Omega (2010, is no exception in that its author engages once more in the exercise of stripping away all surfaces so as to let us see into the terror of what he calls “makeshift reality”—his characters’ and ours. The claims I advance and substantiate in the essay, refer to (1 how a fluid chronology sustained by framing devices adds to the understanding of the construct of a novel/film in progress; (2 how the shifting narrative perspective ensures a vivisectionist’s look into the body of life/death/world. Don DeLillo’s novel is another terrifying X-ray of war/life/death as agonizing nothingness which literature in its ‘late-phase’ is meant to cure.

  2. 77 FR 64973 - Don W. Gilbert Hydro Power, LLC; Notice of Application Accepted for Filing With the Commission...

    Science.gov (United States)

    2012-10-24

    ... Energy Regulatory Commission Don W. Gilbert Hydro Power, LLC; Notice of Application Accepted for Filing.... Date filed: May 30, 2012. d. Applicant: Don W. Gilbert Hydro Power, LLC. e. Name of Project: Gilbert... to: Federal Power Act, 16 U.S.C. 791(a)-825(r). (2006). h. Applicant Contact: Don W. Gilbert and...

  3. 77 FR 37031 - Don W. Gilbert Hydro Power, LLC; Notice of Application Tendered for Filing With the Commission...

    Science.gov (United States)

    2012-06-20

    ... Energy Regulatory Commission Don W. Gilbert Hydro Power, LLC; Notice of Application Tendered for Filing... W. Gilbert Hydro Power, LLC. e. Name of Project: Gilbert Hydroelectric Project. f. Location: The....] h. Applicant Contact: Don W. Gilbert and DeAnn G. Somonich, Don W. Gilbert Hydro Power, LLC,...

  4. Hexokinase plays a critical role in deoxynivalenol (DON) production and fungal development in Fusarium graminearum.

    Science.gov (United States)

    Zhang, Leigang; Li, Baicun; Zhang, Yu; Jia, Xiaojing; Zhou, Mingguo

    2016-01-01

    Fusarium graminearum, the causal agent of Fusarium head blight, is a common pathogen on small grain cereals worldwide and produces various trichothecenes [deoxynivalenol (DON) is predominant] during infection. A previous study has revealed that DON production is positively correlated with the occurrence of carbendazim (MBC) resistance. Here, we identified and characterized two putative genes encoding hexokinase in F. graminearum (FgHXK1 and FgHXK2), which is a rate-limiting enzyme in DON biosynthesis. The expression level of hexokinase genes and the production of pyruvate, which is the precursor of DON, were up-regulated in the MBC-resistant strain, indicating that hexokinase genes might be involved in increased DON production. Phylogenetic and comparative analyses indicated that FgHXK1 was the predominant hexokinase gene. Gene disruption showed that ΔFgHXK1 severely affected DON production, indicating that FgHXK1 played a role in the regulation of DON biosynthesis. Morphological characterization showed that ΔFgHXK1 led to inhibited vegetative growth and conidiation. Sensitivity tests to MBC and various stresses indicated that both ΔFgHXK1 and ΔFgHXK2 mutants showed no significant difference from parental strains. Pathogencity assays showed that ΔFgHXK1 mutants lost virulence on wheat head and corn stigma; however, they showed no change in sexual reproduction. The FgHXK1-overexpressing transformants were obtained subsequently. Their pyruvate and DON production was confirmed to be increased, indicating that FgHXK1 positively regulated DON biosynthesis. Although additional defects appeared in overexpression mutants, MBC sensitivity showed no change. All of the results indicated that the transcriptional level of FgHXK1 regulated DON biosynthesis, but showed no direct relationship with MBC resistance.

  5. When cell biology meets theory

    Science.gov (United States)

    Gonzalez-Gaitan, Marcos

    2015-01-01

    Cell biologists now have tools and knowledge to generate useful quantitative data. But how can we make sense of these data, and are we measuring the correct parameters? Moreover, how can we test hypotheses quantitatively? To answer these questions, the theory of physics is required and is essential to the future of quantitative cell biology. PMID:26416957

  6. When cell biology meets theory.

    Science.gov (United States)

    Gonzalez-Gaitan, Marcos; Roux, Aurélien

    2015-09-28

    Cell biologists now have tools and knowledge to generate useful quantitative data. But how can we make sense of these data, and are we measuring the correct parameters? Moreover, how can we test hypotheses quantitatively? To answer these questions, the theory of physics is required and is essential to the future of quantitative cell biology.

  7. 黑暗和光照对长春花培养细胞生长和生理生化特性的影响%The Effects of Dark and Light on Growth and Physiological and Biochemical Characteristics in Cell Culture of Catharanthus roseus(L.)G.Don

    Institute of Scientific and Technical Information of China (English)

    郭胜娟; 刘树楠; 冯玲玲; 周吉源

    2004-01-01

    以长春花(Catharanthus roseus (L.)G.Don)幼嫩叶片为外植体,在MS+NAA 0.5 mg/L+2,4-D 0.5 mg/L+6 BA 2.0 mg/L培养基上诱导形成愈伤组织,愈伤组织置于不同条件下培养.结果表明,黑暗和光照下,培养细胞的生长周期为27 d;过氧化物酶(POD)活性和超氧化物歧化酶(SOD)活性均出现2个峰值.对长春花总生物碱含量和总产量亦做了研究,结果发现最佳的收获时期为第27 d.

  8. Doña Clara e don Luis: l’ennesima 'questione amorosa' all’interno della "venta de Palomeque" (Don Quijote, I, 32-47

    Directory of Open Access Journals (Sweden)

    A. Candeloro

    2012-08-01

    Full Text Available ITDoña Clara e don Luis rappresentano l’ultima coppia d’innamorati che raggiunge la locanda di Palomeque, all’interno di quella serie di disavventure e riconoscimenti insperati che danno vita allo “escandaloso happy end” – come lo definirà ironicamente Juan Benet – nel quale si risolve l’azione principale connessa alle numerose trame secondarie  sviluppate proprio nello spazio “scenico” della famosa locanda. Scopo di questo studio è quello di analizzare le caratteristiche e le problematiche legate alla “questione amorosa” di doña Clara e don Luis, il modo in cui nasce e si sviluppa tale intreccio e le tecniche narrative attraverso cui Cervantes ricollega e amalgama questa storia d’amore a quelle delle altre coppie presenti nella conclusione della Primera Parte del Quijote.ENDoña Clara and don Luis are the last couple of lovers arriving to Palomeque's inn. They are the umpteenth romantic couple in a string of episodes based on several recognition scenes between different couples.  That's why they contribute to reach that "scandalous happy end" – as Juan Benet ironically defined it –, through which the main action of the novel's plot comes to a conclusion and allows to finish, at the same time, all the secondary plots at the inn. This report aims to analyze the characteristics and problems related to "the love issue" between Doña Clara and don Luis and, in special, all the particular narrative techniques which Cervantes uses to link this "love story" with other couples in the first part of Don Quijote.Keywords: Cervantes – Don Quijote – narrative techniques – Doña Clara and Don Luis – love question

  9. El viaje de Don Pero Tafur (1436-1439

    Directory of Open Access Journals (Sweden)

    Villalba Ruíz de Toledo, Francisco Javier

    2005-04-01

    Full Text Available En este breve análisis del viaje de Don Pero Tafur, hemos querido poner el acento en la percepción que tiene de Oriente un representante de la hidalguía castellana de la segunda mitad del siglo XV, para lo que resulta inestimable su relato autobiográfico. De ahí que únicamente nos detengamos en el recorrido que le lleva a las islas griegas. Tierra Santa, Egipto y el mundo bizantino y turco. Como aspecto complementario de esa atracción oriental para el hombre europeo de la época, dedicamos un espacio al encuentro del aventurero andaluz con el célebre mercader italiano, Niccolò dei Conti.…

  10. Don DeLillo’s Mapping of the City

    Directory of Open Access Journals (Sweden)

    Stipe Grgas

    2005-06-01

    Full Text Available Taking as his point of departure the immense significance the city has for understanding the present moment and the special relationship the city has had with the novel, the author gives a reading of Don DeLillo and the way his work has engaged the city of New York. Focusing upon his last two novels, Underworld and Cosmopolis, the author describes how these two novels narrate the transformations the American city has undergone during the second part of the twentieth century. The bulk of his analysis deals with the function the Prologue flashback of the Bronx has in the earlier novel and the transformed city of late capitalism in his last text. The author concludes his reading by pointing out how DeLillo’s novels not only provide fictional accounts of what has occurred in the urban sphere but how they provide evidence of the difficulty of representing the contemporary world and how they foreground urgent political considerations.

  11. Workshop on trichothecenes with a focus on DON: summary report

    DEFF Research Database (Denmark)

    Larsen, John Christian; Hunt, Josephine; Perrin, Irene;

    2004-01-01

    Natural Toxin Task Force therefore organised a workshop on trichothecenes with a special focus on deoxynivalenol (DON). A number of experts reviewed the current knowledge on trichothecenes with respect to occurrence, including aspects of mould growth, toxin formation, storage and effects of processing......A number of mycotoxins of the class of trichothecenes are produced by a variety of Fusarium fungi commonly found on cereals. Unfavourable weather conditions may lead to a high level of Fusarium infections in crops such as wheat and correspondingly high trichothecene contents. The ILSI Europe......; prevention; analytical methodologies, including sampling; surveillance and exposure assessments; and toxicology and risk assessment. A number of recommendations were given under the headings: prevention, sampling and analytical methods, exposure assessment, and toxicology. Gaps in knowledge were also...

  12. Studies on phytosterol content of Parkia roxburgii G. Don.

    Directory of Open Access Journals (Sweden)

    DJADJAT TISNADJAJA

    2006-01-01

    Full Text Available Kedawung (Parkia roxburgii G. Don. is one of plants that originally could be found in almost all parts of Java Island. Due to lack of attention, at present situation this plant could be categorized as an endangered species. This plant species distributed widely in Africa. In several African countries, this plant has an important position either as food resource or alternative medicine. In Indonesia, traditionally kedawung tree often use as medicine, especially as part of “jamu gendong” formulation. This work was focused on the study of phytosterol content and it distribution. The research result show that almost all parts of kedawung tree have a significant content of phytosterol, which is dominated by beta-sitosterol. The highest beta-sitosterol content was founded in the stem of leaf (35.24% (w/w and pod (29.67% (w/w.

  13. Efectos embriotóxicos de Picrosia longifolia Don (Asteraceae

    Directory of Open Access Journals (Sweden)

    Victor Benavides

    2014-06-01

    Full Text Available Se ha evaluado los probables efectos tóxicos del extracto acuoso de Picrosia longifolia Don “Achicoria” sobre el desarrollo de embriones preimplantacionales de ratón. Extracto acuoso de P. longifolia al 20%, 10% y 0% fue suministrado a ratonas preñadas hasta las 86 horas postcópula (hpc. Al evaluar el estadio embrionario se encontró que el extracto acuoso de P. longifolia al 10% y 20% causó retraso en el desarrollo embrionario. También se observó una disminución en la viabilidad embrionaria en los grupos tratados. Tanto la disminución de viabilidad como el retraso en el desarrollo se produjo en una forma dosis dependiente.

  14. Scientific Communication for Positive Action: Do's and Don'ts

    Science.gov (United States)

    Sanquini, A.; Wood, M. M.

    2015-12-01

    Natural hazard presentations often highlight disasters that may ensue from natural processes when mitigation or preparedness actions are not taken. Examples include images of raging fires, collapsed buildings, and flooded urban areas. Research has shown that this makes presentations more interesting and more memorable. Such images are the stock and trade of disaster reporting by the media. Unfortunately, it may also trigger avoidance and denial in the audience, resulting in preparedness reduction; the opposite effect of what may have been intended by the speaker. Recent social research has provided insight into a better approach. The theory of communicating actionable risk posits that people will take action against hazards when they know what to do, think it would work, and know someone who did it. This approach was recently applied in an intervention designed to motivate earthquake-resistant construction in Kathmandu Valley, Nepal. Social theory further suggests that a tendency towards action is strengthened by hearing a consistent message over time, and by providing the audience with an appropriate opportunity to seek out relevant information. This presentation shows how, by taking this transdisciplinary step, scientists can make small changes in their hazard communication, thereby acting as positive influencers of change. A summary of "do's" and "don'ts" is given for reference. 1. Show examples of what to do. 2. Show effectiveness of actions. 3. Give sense of knowing someone who did it. 4. Deliver consistent message, repeatedly. 5. Give opportunity for more information. DON'T: 1. Make the disaster the star. 2. Contradict other communications.

  15. Anatomical characters of the phyllode and stem of Acacia podalyriifolia A. Cunn. ex G. Don (Fabaceae

    Directory of Open Access Journals (Sweden)

    M.R. Duarte

    2005-03-01

    Full Text Available The Acacia genus has presented various secondary metabolites, such as tannins, flavonoids, alkaloids and gums. Preparations from different species have been applied for diabetes, gastrointestinal disorders and inflammatory diseases in the traditional medicine and have demonstrated cytotoxic, antimicrobial and antiparasitic activities. Acacia podalyriifolia A. Cunn. ex G. Don (Fabaceae is a small wood, indigenous to Australia and cultivated worldwide for its ornamental feature. This work aimed to characterize the anatomy of the phyllode and stem, in order to contribute to the species identification. The botanical material was fixed, sectioned and prepared according to usual light and scanning microtechniques. The epidermal cells, in surface view, are polygonal and coated with striate and thick cuticle, and filaments of epicuticular wax. Paracytic stomata and unicellular non-glandular trichomes are seen. Palisade and ground parenchymas, and minor collateral bundles with xylem directed alternately to upper and lower sides occur in the blade. The midrib shows two collateral bundles facing each other. The stem, in incipient secondary growth, exhibits epidermis, annular collenchyma, sclerenchymatic sheath and collateral vascular organization. Cells containing phenolic compounds and prisms of calcium oxalate are observed.

  16. 有机氮对大亚湾亚历山大藻种群生长的促进作用%Facilitation of DON on population growth ofAlexandrium sp.in Daya Bay

    Institute of Scientific and Technical Information of China (English)

    徐宁; 王萌; 孙凯峰; 胡章喜; 段舜山

    2012-01-01

    2008年1~12月对大亚湾澳头养殖海域溶解有机氮(DON)组成、含量与亚历山大藻种群动态进行了周年调查,同步监测了无机营养盐、水温、盐度等主要环境因子.结果显示,大亚湾澳头海域DON水平4~6月较高,最大值达21.27μmolN/L,其余月份较低,全年平均浓度为7.44μmolN/L.尿素是DON的重要组分,全年平均浓度为1.98μmolN/L,约占DON的20%~30%.可溶性游离氨基酸(DFAA)波动较大,介于1~5μmolN/L.4~6月亚历山大藻种群维持较高密度.4月21日澳头海域发生亚历山大藻水华,最高细胞密度达到3319cells/mL.DON和尿素浓度高峰与亚历山大藻密度高峰同步出现,水华消散后DON和尿素浓度分别大幅下降至高峰期的23.84%和62.86%.统计结果显示,表征有机污染程度的DON、尿素和CODMn与亚历山大藻种群密度具有显著正相关关系(p<0.05).DON含量的增加能够促进亚历山大藻的生长,并在温度、盐度等环境条件适宜的情况下可能成为赤潮暴发的重要诱因.%Population dynamics of Alexandrium sp. And DON were investigated in aquaculture areas of Daya Bay from January' to December in 2008 while environmental factors including nutrients, water temperature and salinity were investigated simultaneously. The concentration of DON was higher from April to June than that in other months. The maximum value for DON was 21.27umolN/L while the annual average value was 7.44umol N/L. Urea was one of the most important components of DON. The annual average concentration of urea was 1.98umol N/L which represented about 20%~30% of DON. DFAA fluctuated significantly ranging from 1 to 5umol N/L. Cell densities of Alexandrium sp. Were found higher from April to June than those in other months. Alexandrium sp. Bloom was observed in Aotou aquaculture area with the highest cell density of 3319 cells/mL on April 21. The concentrations of DON,and urea and the cell density of Alexandrium sp. Peaked

  17. Don Edwards San Francisco Bay National Wildlife Refuge: Annual Narrative Report: Calendar Year 2000

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual narrative report for Don Edwards San Francisco Bay NWR, Antioch Dunes NWR, Farallon NWR, San Pablo Bay NWR, Ellicot Slough NWR, Salinas River NWR, and...

  18. Botulism protocols for the Don Edwards San Francisco Bay National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This plan gives a basic description of what avian botulism is and the locations on the Don Edwards San Francisco Bay National Wildlife Refuge where botulism...

  19. 4 in 10 Popular Sunscreens Don't Meet Sun Safety Standards

    Science.gov (United States)

    ... html 4 in 10 Popular Sunscreens Don't Meet Sun Safety Standards: Study Biggest problem with products ... products sold in the United States fail to meet basic sun safety guidelines, new research shows. The ...

  20. The Trail Inventory of Don Edwards San Francisco Bay National Wildlife Refuge [Cycle 1

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The purpose of this report is to create a baseline inventory of all nonmotorized trails on Don Edwards San Francisco Bay National Wildlife Refuge. Trails in this...

  1. STS-42 Pilot Oswald dons CCA prior to egress exercises in JSC's WETF pool

    Science.gov (United States)

    1991-01-01

    STS-42 Discovery, Orbiter Vehicle (OV) 103, Pilot Stephen S. Oswald, wearing a launch and entry suit (LES), dons his communications carrier assembly (CCA) prior to launch emergency egress (bailout) exercises in JSC's Weightless Environment Training Facility (WETF) Bldg 29.

  2. Regional economic impacts of current and proposed management alternatives for Don Edwards National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report first presents a description of the local community and economy near the Don Edwards San Francisco Bay NWR. Next, the report describes methods used to...

  3. Pilot Fullerton dons EES anti-gravity suit lower torso on middeck

    Science.gov (United States)

    1982-01-01

    Pilot Fullerton dons ejection escape suit (EES) anti-gravity (anti-g) suit lower torso on forward port side middeck above potable water tank. Anti-g suit is an olive drab inner garment that complements EES.

  4. The Trail Inventory of Don Edwards San Francisco Bay NWR [Cycle 2

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The purpose of this report is to create a baseline inventory of all non-motorized trails on Don Edwards San Francisco Bay National Wildlife Refuge. Trails in this...

  5. [Don Edwards San Francisco Bay National Wildlife Refuge: Final Comprehensive Conservation Plan

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This Comprehensive Conservation Plan (CCP) executive summary was written to guide management on Don Edwards San Francisco National Wildlife Refuge for the next 15...

  6. Biocompatibility and anti-microbiological activity characterization of novel coatings for dental implants: A primer for non-biologists

    Directory of Open Access Journals (Sweden)

    Thomas K Monsees

    2016-08-01

    Full Text Available With regard to biocompatibility, the cardinal requirement for dental implants and other medical devices that are in long-term contact with tissue is that the material does not cause any adverse effect to the patient. To warrant stability and function of the implant, proper osseointegration is a further prerequisite. Cells interact with the implant surface as the interface between bulk material and biological tissue. Whereas structuring, deposition of a thin film or other modifications of the surface are crucial parameters in determining favorable adhesion of cells, corrosion of metal surfaces and release of ions can affect cell viability. Both parameters are usually tested using in vitro cytotoxicity and adhesion assays with bone or fibroblasts cells. For bioactive surface modifications, further tests should be considered for biocompatibility evaluation. Depending on the type of modification, this may include analysis of specific cell functions or the determination of antimicrobial activities. The latter is of special importance as bacteria and yeast present in the oral cavity can be introduced during the implantation process and this may lead to chronic infections and implant failure. An antimicrobial coating of the implant is a way to avoid that. This review describes the essential biocompatibility assays for evaluation of new implant materials required by ISO 10993 and also gives an overview on recent test methods for specific coatings of dental implants.

  7. MENINGKATKAN KEMAMPUAN MEMBACA SISWA DENGAN MENGGUNAKAN MEDIA GAMBAR KELAS ISD FRATER DON BOSCO TARAKAN

    OpenAIRE

    Yuliana Kaha

    2011-01-01

    Yuliana Kaha, 2011, Meningkatkan Kemampun Membaca Siswa Dengan Menggunakan Media Gambar Kelas 1 SD Frater Don Bosco Tarakan Tahun Pembelajaran 2010/ 2011. Skripsi Jurusan Pendidikan Guru Sekolah Dasar Universitas Borneo Tarakan, Pembimbing: (1) Sungkono,S.Pd,MA(2) Drs. Herdiansyah, M.Si. Skripsi ini berjudul Meningkatkan Kemampuan Membaca Siswa Dengan Menggunakan Media Gambar Kelas I SD Frater Don Bosco Tarakan Tahun pembelajaran 2010/ 2011. Skripsi ini merupakan hasil karya akademis untuk Me...

  8. Stability of DON and OTA during the breadmaking process and determination of process and performance criteria

    OpenAIRE

    Vidal, A.; Morales-Valle, H.; Sanchis, V.; Ramos, A. J.; Marín, S.

    2014-01-01

    The fate of deoxynivalenol (DON) and ochratoxin A (OTA) during the breadmaking process was studied. In particular, toxin content was analysed in mixed baking ingredients before kneading, after fermentation and proofing, and finally after baking. Fermentation and proofing were carried out at 30 C for 1 h, while baking was performed at different temperature levels (from 170 to 210 C) and baking times from 45 to 135 min, in a full factorial design. DON increased from unkneaded mix to fermented d...

  9. Mixing regime as a key factor to determine DON formation in drinking water biological treatment.

    Science.gov (United States)

    Lu, Changqing; Li, Shuai; Gong, Song; Yuan, Shoujun; Yu, Xin

    2015-11-01

    Dissolved organic nitrogen (DON) can act as precursor of nitrogenous disinfection by-products formed during chlorination disinfection. The performances of biological fluidized bed (continuous stirred tank reactor, CSTR) and bio-ceramic filters (plug flow reactor, PFR) were compared in this study to investigate the influence of mixing regime on DON formation in drinking water treatment. In the shared influent, DON ranged from 0.71mgL(-1) to 1.20mgL(-1). The two biological fluidized bed reactors, named BFB1 (mechanical stirring) and BFB2 (air agitation), contained 0.12 and 0.19mgL(-1) DON in their effluents, respectively. Meanwhile, the bio-ceramic reactors, labeled as BCF1 (no aeration) and BCF2 (with aeration), had 1.02 and 0.81mgL(-1) DON in their effluents, respectively. Comparative results showed that the CSTR mixing regime significantly reduced DON formation. This particular reduction was further investigated in this study. The viable/total microbial biomass was determined with propidium monoazide quantitative polymerase chain reaction (PMA-qPCR) and qPCR, respectively. The results of the investigation demonstrated that the microbes in BFB2 had higher viability than those in BCF2. The viable bacteria decreased more sharply than the total bacteria along the media depth in BCF2, and DON in BCF2 accumulated in the deeper media. These phenomena suggested that mixing regime determined DON formation by influencing the distribution of viable, total biomass, and ratio of viable biomass to total biomass. PMID:25585870

  10. Buy, don't build -- What does that mean for a software developer?

    International Nuclear Information System (INIS)

    The buzz phrase of the 1990's for the petroleum software industry has become ''buy, don't build.'' For an end user in an oil company, this generally means acquiring application software rather than developing it internally. The concept of buy, don't build can also apply for a software developer. Purchasing software toolkit components can expedite the development of an application as well as reduce future support requirements

  11. Antidiabetic and Antioxidant Properties of Alkaloids from Catharanthus roseus (L.) G. Don

    OpenAIRE

    Won Fen Wong; Shiau-Chuen Cheah; Mohd Rais Mustafa; Mohammadjavad Paydar; Aditya Arya; Soon Huat Tiong; Chung Yeng Looi; Khalijah Awang; Hazrina Hazni

    2013-01-01

    Catharanthus roseus (L.) G. Don is a herbal plant traditionally used by local populations in India, South Africa, China and Malaysia to treat diabetes. The present study reports the in vitro antioxidant and antidiabetic activities of the major alkaloids isolated from Catharanthus roseus (L.) G. Don leaves extract. Four alkaloids—vindoline I, vindolidine II, vindolicine III and vindolinine IV—were isolated and identified from the dichloromethane extract (DE) of this plant’s leaves. DE and comp...

  12. Fuel elements assembling for the DON project exponential experience; Montaje de los elementos combustibles para la experiencia exponencial del proyecto DON

    Energy Technology Data Exchange (ETDEWEB)

    Anca Abati, R. de

    1966-07-01

    It is described the fuel unit used in the DON exponential experience, the manufacturing installments and tools as well as the stages in the fabrication.These 74 elements contain each 19 cartridges loaded with synterized urania, uranium carbide and indium, gold, and manganese probes. They were arranged in calandria-like tubes and the process-tube. This last one containing a cooling liquid simulating the reactor organic. Besides being used in the DON reactor exponential experience they were used in critic essays by the substitution method in the French reactor AQUILON II. (Author) 6 refs.

  13. Occurrence of Deoxynivalenol (DON in wheat flours in Guilan Province, northern Iran

    Directory of Open Access Journals (Sweden)

    Reza Kazemi Darsanaki

    2015-02-01

    Full Text Available [b]Introduction and objective.[/b] Deoxynivalenol (DON is one of several mycotoxins produced by certain Fusarium species that frequently infect wheat, corn, rice, oats, barley and other grains in the field or during storage. Guilan, Golestan and Mazandaran areas are located in the North Iran with favorite conditions for [i]Fusarium[/i] growth. DON affects animal and human health causing vomiting, acute temporary nausea, diarrhea, abdominal pain, headache, dizziness, and fever. [b]Materials and methods[/b]. In this study, a total of 96 wheat flour samples of different types were collected during summer 2013 from six flour mills in Northern Iran. All samples were analyzed for DON by enzyme-linked immunosorbent assay. [b]Results[/b]. DON was detected in 80 out of 96 wheat flour samples (83.33%, at levels ranging from 23 to1270 µg/kg. The maximum contamination level of DON (1270 μg/kg was found in whole flour while the minimum value (23 μg/kg was registered in debranned flour. The mean of contamination was 630.53 µg/kg. [b]Conclusions[/b]. According to results obtained, incidence and contamination levels of DON, seem to be a serious problem for public health. Therefore, cereal and cereal based foods should be controlled for the presence of toxins, storage conditions and moisture content, which is considered a major factor in the growth of the [i]Fusarium[/i] fungi.

  14. Consumption as Sign in Don DeLillo’s Cosmopolis

    Directory of Open Access Journals (Sweden)

    Seyyede Fateme Mirbabazade

    2013-11-01

    Full Text Available As one of the foremost American postmodern authors, Don DeLillo tends to focus on construction of postmodern subject’s consciousness through media, technology and consumption. Nevertheless, despite many affinities between Baudrillard and DeLillo, many researches which have been done on his works have failed to theoretically analyze them from Baudrillardian perspective. Although there is affinity between Baudrillard and DeLillo, there is dissimilarity as well. While Baudrillard believes that that there is no escape for individual from the ideology of the system, DeLillo claims that there is hope for change and revolution for the individuals by their action in society, particularly by the means of art, to be more  than a cog in the machine of ideology of system. Baudrillard, as one of the most influential postmodern theorists, defines consumption as organized control of sign and one of the many ways any subject is assimilated in any given society. For Baudrillard the logic of consumption is based on social differentiation. By the logic of social differentiation, individuals distinguish themselves and obtain social prestige and standing through the purchase and use of consumer goods. In his theorization about the concept of need and value, Baudrillard finds Marx’s logics of use value (utility and functionality and exchange value (economic value inadequate to include the whole function of an object and adds the logic of sign value. Baudrillard asserts that in consumption economic exchange value (money is transformed into sign exchange value (prestige, etc.; yet this functioning is maintained by the use value. The only way out of this dilemma, Baudrillard claims, is to return to symbolic exchange which is neither a conception nor an agency, but an act of exchange and a social relation and the only approach to real human communication and understanding. Therefore this research attempts to highlights those affinities in DeLillo’s first post-9

  15. Study on the Induction of Polyploid Bulb of Fritillaria Cirrhosa D. Don%卷叶贝母多倍体鳞茎诱导方法研究

    Institute of Scientific and Technical Information of China (English)

    王跃华; 胡胜铃; 代勇; 蒋婷婷; 付伟; 杨军

    2011-01-01

    [ Objective ] Rolyploid bulb of Fritillaria cirrhosa D. Don is introduced by tissue culture technique. [ Methods ] The compact callus cell mass of Fritillaria cirrhosa D. Don which are differentiation cultured beforehand are soaked in colchicine solution, and then cultured in the differential medium of MS + 6-BA 1.0 mg/L + NAA 0.3 mg/L. Polyploid bulb are obtained by induction differentiation culture. [ Result] Colchicine solution of 1.2 g/L is used to douse the compact callus cell mass of Fritillaria cirrhosa D. Don for 24 h. The highest inductivity of polyploid bulb was 83.3%. [ Conclusion] Chromosome number is 2n =4x =48 and the total alkaloids content is detected to be 0. 249%. It indicates that it is feasible to obtain high quality and high yield of Fritillaria cirrhosa D. Don polyploid bulb by tissue culture technique.%[目的]利用组织培养技术快速诱导生成卷叶贝母(Fritillaria cirrhosa D.Don)多倍体鳞茎.[方法]用一定浓度的秋水仙素溶液浸泡经预分化培养的卷叶贝母紧密愈伤组织团块,再经过诱导分化培养获得多倍体鳞茎.[结果]用浓度为1.2 g/L的秋水仙素溶液浸泡处理卷叶贝母紧密愈伤组织团块24 h后,接种在Ms+6-BA 1.0 mg/L+NAA 0.3 mg/L的分化培养基上培养,其多倍体鳞茎的诱导率最高为83.3%,细胞染色体数为2n=4x=48,总生物碱含量为0.249%.[结论]用组织培养技术获得优质高产卷叶贝母多倍体鳞茎的方法是可行的.

  16. Identification of a novel human deoxynivalenol metabolite enhancing proliferation of intestinal and urinary bladder cells

    Science.gov (United States)

    Warth, Benedikt; Del Favero, Giorgia; Wiesenberger, Gerlinde; Puntscher, Hannes; Woelflingseder, Lydia; Fruhmann, Philipp; Sarkanj, Bojan; Krska, Rudolf; Schuhmacher, Rainer; Adam, Gerhard; Marko, Doris

    2016-01-01

    The mycotoxin deoxynivalenol (DON) is an abundant contaminant of cereal based food and a severe issue for global food safety. We report the discovery of DON-3-sulfate as a novel human metabolite and potential new biomarker of DON exposure. The conjugate was detectable in 70% of urine samples obtained from pregnant women in Croatia. For the measurement of urinary metabolites, a highly sensitive and selective LC-MS/MS method was developed and validated. The method was also used to investigate samples from a duplicate diet survey for studying the toxicokinetics of DON-3-sulfate. To get a preliminary insight into the biological relevance of the newly discovered DON-sulfates, in vitroexperiments were performed. In contrast to DON, sulfate conjugates lacked potency to suppress protein translation. However, surprisingly we found that DON-sulfates enhanced proliferation of human HT-29 colon carcinoma cells, primary human colon epithelial cells (HCEC-1CT) and, to some extent, also T24 bladder cancer cells. A proliferative stimulus, especially in tumorigenic cells raises concern on the potential impact of DON-sulfates on consumer health. Thus, a further characterization of their toxicological relevance should be of high priority. PMID:27659167

  17. ‘Early Psychosis’ as a Mirror of Biologist Controversies in Post-WarGerman, Anglo-Saxon and Soviet Psychiatry

    Directory of Open Access Journals (Sweden)

    Lara eRzesnitzek

    2013-07-01

    Full Text Available The English term ‘early psychosis’ was coined in the 1930s to refer to feelings of irritability, loss of concentration, hypochondriac ideas, moodiness and lassitude that were seen to precede the onset of clear-cut hallucinations and delusions. The history of thinking about ‘early psychosis’ under names such as ‘latent’, ‘masked’, ‘mild’, ‘simple’ or ‘sluggish’ schizophrenia before World War II and afterwards on the different sides of the Wall and the Iron Curtain reveals ‘early psychosis’ as a mirror of quite aged international biologist controversies that are still alive today and to the same extent as they are misunderstood, are influential in their implications in today’s psychiatry.

  18. Transtibial prosthetic suspension: Less pistoning versus easy donning and doffing

    Directory of Open Access Journals (Sweden)

    Hossein Gholizadeh, MEngSc

    2012-12-01

    Full Text Available Poor suspension increases slippage of the residual limb inside the socket during ambulation. The main purpose of this article is to evaluate the pistoning at the prosthetic liner-socket interface during gait and assess patients’ satisfaction with two different liners. Two prostheses with seal-in and locking liners were fabricated for each of the 10 subjects with transtibial amputation. The Vicon motion system was used to measure the pistoning during gait. The subjects were also asked to complete a Prosthesis Evaluation Questionnaire. The results revealed higher pistoning inside the socket during gait with the locking liner than with the seal-in liner (p < 0.05. The overall satisfaction with the locking liner was higher (p < 0.05 because of the relative ease with which the patients could don and doff the device. As such, pistoning may not be the main factor that determines patients’ overall satisfaction with the prosthesis and other factors may also contribute to comfort and satisfaction with prostheses. The article also verifies the feasibility of the Vicon motion system for measuring pistoning during gait.

  19. Why change programs don't produce change.

    Science.gov (United States)

    Beer, M; Eisenstat, R A; Spector, B

    1990-01-01

    Faced with changing markets and tougher competition, more and more companies realize that to compete effectively they must transform how they function. But while senior managers understand the necessity of change, they often misunderstand what it takes to bring it about. They assume that corporate renewal is the product of company-wide change programs and that in order to transform employee behavior, they must alter a company's formal structure and systems. Both these assumptions are wrong, say these authors. Using examples drawn from their four-year study of organizational change at six large corporations, they argue that change programs are, in fact, the greatest obstacle to successful revitalization and that formal structures and systems are the last thing a company should change, not the first. The most successful change efforts begin at the periphery of a corporation, in a single plant or division. Such efforts are led by general managers, not the CEO or corporate staff people. And these general managers concentrate not on changing formal structures and systems but on creating ad hoc organizational arrangements to solve concrete business problems. This focuses energy for change on the work itself, not on abstractions such as "participation" or "culture." Once general managers understand the importance of this grass-roots approach to change, they don't have to wait for senior management to start a process of corporate renewal. The authors describe a six-step change process they call the "critical path."

  20. Authority as paradox: the transformations of Don Quijote.

    Science.gov (United States)

    Priel, Beatriz

    2006-12-01

    The author's contention is that the analysand's temporary attribution of authority to the analyst is inherent in the analytic situation; this is seen as a transitional and paradoxical form of authority pertaining neither to internal nor external reality, but dwelling in the analytic third. The author proposes a conceptualization of psychoanalytic authority as a form of aesthetic authority according to Gadamer's definitions. While the scientific and hermeneutic codes for the understanding of authority in psychoanalysis assume that the main issue at stake is the delimitation of the objectivity or the subjectivity of the analyst's knowledge, this aesthetic perspective centres on the analysand's attribution of a claim of truth to analytic interpretations, and on the experience of recognition. The experience of recognition of a possible truth is particular and context-bound, as well as self-transformational. A reading of three episodes from Cervantes's The history of Don Quixote de la Mancha illuminates the transitional and paradoxical character of aesthetic authority within a transformational dialogue. These episodes are read as dramatizations of different positions vis-à-vis the paradoxical authority that characterizes transformational dialogues.

  1. Catharanthus roseus (L. G. Don - plant regeneration and alkaloids content

    Directory of Open Access Journals (Sweden)

    Mirosława Furmanowa

    2014-02-01

    Full Text Available We describe here a regeneration of plantlets of Catharanthus roseus (L. G. Don from shoot tips and axillary buds. Shoot tips were excised from 7-day-old seedlings and were incubated in solid Nitsch and Nitsch (NN medium supplemented with kinetin, benzyladenine (BA, indole-3-butyric acid (IBA and β-indolylacetic acid (IAA in various combinations. After two months in culture, regenerated rooted plantlets were cut and transferred to a new medium; the explants contained shoot tips or axillary buds. Four passages were done. We obtained about 200 rooting plantlets from one seedling. Then the plantlets were transferred to the soil and they grew under a foil tent. After five months of vegetation they were collected, dried and weighed. Chemical investigations of leaves of these plants were done. The vindoline and catharanthine were dominant alkaloids in the juvenile stage of plants (before blooming. Total amount of alkaloids, equal 2.95%, was gravimetrically determined in leaves of plants, after 4th passage, regenerated in vitro on NN medium supplemented with kinetin and IBA.

  2. Saponins isolated from Allium chinense G. Don and antitumor-promoting activities of isoliquiritigenin and laxogenin from the same drug.

    Science.gov (United States)

    Baba, M; Ohmura, M; Kishi, N; Okada, Y; Shibata, S; Peng, J; Yao, S S; Nishino, H; Okuyama, T

    2000-05-01

    Investigation of the Chinese crude drug "Xiebai," the bulbs of Allium chinense G. Don (Liliaceae), led to the isolation of 2 saponins, xiebai-saponin I (laxogenin 3-O-beta-xylopyranosyl (1-->4)-[alpha-arabinopyranosyl (1-->6)-beta-glucopyranoside) (1) and laxogenin 3-O-alpha-arabinopyranosyl (1-->6)-beta-glucopyranoside (2), and the aglycone, laxogenin (3), together with 2 chalcones, isoliquiritigenin (4) and isoliquiritigenin-4-O-glucoside (5), and beta-sitosterol glucoside (6). Compounds 1-5 were tested in vitro for their inhibitory effect on the 12-O-tetradecanoylphorbol-13-acetate (TPA)-stimulated 32Pi-incorporation into phospholipids of HeLa cells. In addition to this, laxogenin (3) was proven to have an antitumor-promoting activity in a two-stage lung carcinogenesis experiment. PMID:10823685

  3. Risk Acceptance Personality Paradigm: How We View What We Don't Know We Don't Know

    Science.gov (United States)

    Massie, Michael J.; Morris, A. Terry

    2011-01-01

    The purpose of integrated hazard analyses, probabilistic risk assessments, failure modes and effects analyses, fault trees and many other similar tools is to give managers of a program some idea of the risks associated with their program. All risk tools establish a set of undesired events and then try to evaluate the risk to the program by assessing the severity of the undesired event and the likelihood of that event occurring. Some tools provide qualitative results, some provide quantitative results and some do both. However, in the end the program manager and his/her team must decide which risks are acceptable and which are not. Even with a wide array of analysis tools available, risk acceptance is often a controversial and difficult decision making process. And yet, today's space exploration programs are moving toward more risk based design approaches. Thus, risk identification and good risk assessment is becoming even more vital to the engineering development process. This paper explores how known and unknown information influences risk-based decisions by looking at how the various parts of our personalities are affected by what they know and what they don't know. This paper then offers some criteria for consideration when making risk-based decisions.

  4. The Authors of the Ingenious Hidalgo Don Quixote of La Mancha

    Directory of Open Access Journals (Sweden)

    Branka Kalenić Ramšak

    2008-12-01

    Full Text Available Even today literary criticism still considers the novel Don Quixote the first modern European novel because it fundamentally changes both the concept of literary creation and the findings regarding literary reception. Miguel de Cervantes Saavedra published the first part of his chivalric novel in 1605 in Madrid with the title El ingenioso hidalgo don Quijote de la Mancha. He published the second part, titled El ingenioso caballero don Quijote de la Mancha, ten years later (i.e., in 1615, again in Madrid. Why did Cervantes change the title in the second part of his novel and thus transform Don Quixote the hidalgo ‘nobleman’ into Don Quixote the caballero ‘knight, nobleman, horseman’? In Spanish literature of Cervantes’ time, writers often borrowed texts from one another, wrote sequels to them, and reworked them into humorous poems, jocular one-act plays, or unusual parodies. The Baroque concept of imitation was not understood as plagiarism, but rather as a positive approach to creativity. One of Cervantes’ most enthusiastic imitators was Alonso Fernández de Avellaneda. He hated Cervantes, but loved Don Quixote so much that he wrote a sequel to it in the form of a chivalric novel. In 1614, Avellaneda published his novel titled Segundo tomo del ingenioso hidalgo don Quijote de la Mancha (The Second Part of the Ingenious Hidalgo of La Mancha in Tarragona. Who is hidden behind the name Alonso Fernández de Avellaneda? To date, literary history has not been able to establish with certainty who the author of this “second part” was; this work represents the greatest literary mystery of all time in Spanish literature. In the 1960s a theory developed among Cervantes experts that for now seems to be the most convincing in determining Avellaneda’s true identity. In his article “ El Quijote y los libros” (Don Quixote and Books of 1969, Martín de Riquer presented the first well-founded hypothesis claiming that the writer Alonso Fern

  5. Pharmacognostical studies of leaves of Combretum albidum G. Don

    Directory of Open Access Journals (Sweden)

    Ashish S Zalke

    2013-01-01

    Full Text Available Background: Combretum albidum Don belonging to family Combretaceae is an unexplored medicinal plant in the Indian medicinal system. According to ethnobotanical information, the leaves are used in the treatment of peptic ulcer and its fruits are used in diarrhoea and dysentery. Stem bark is used in the treatment of jaundice and skin diseases. The problem encountered in standardisation of this medicinal plant is its identification by source. Materials and Methods: The pharmacognostical studies were carried out in terms of organoleptic, macroscopic, microscopic, physicochemical, florescence and phytochemical analysis. Physicochemical parameters such as total ash, moisture content and extractive values are determined by World Health Organization guidelines. The microscopic features of leaf components are observed with Nikon lab photo device with microscopic units. Results: Macroscopically, the leaves are simple, obovate in shape, acuminate apex, entire margin and smooth surface. Microscopically, the leaves showed a large vascular strand that consists of thick walled xylem elements mixed with xylem fibres and phloem which is present in a thin layer along inner and outer portions of xylem. External to the xylem occur a thin line of sclerenchyma. Powder microscopy revealed glandular trichomes in the adaxial epidermal peelings also shows the non-glandular trichomes fairly common in powder and epidermis with anisocytic stomata. Vessels elements are narrow, long, cylindrical and dense multi-seriate bordered pits. Xylem fibres are thin and long, with thick walls, which are lignified. Preliminary phytochemical screening showed the presence of carbohydrate, glycoside, saponin, flavonoid, phytosterols and phenolic compounds. Conclusions: The results of the study can serve as a valuable source of pharmacognostic information as suitable standards for identification of this plant material in future investigations and applications.

  6. From cell biology to the microbiome: An intentional infinite loop.

    Science.gov (United States)

    Garrett, Wendy S

    2015-07-01

    Cell biology is the study of the structure and function of the unit or units of living organisms. Enabled by current and evolving technologies, cell biologists today are embracing new scientific challenges that span many disciplines. The eclectic nature of cell biology is core to its future and remains its enduring legacy.

  7. RICKETS AT THE MEDICI COURT OF FLORENCE: THE CASE OF DON FILIPPINO (1577-1582).

    Science.gov (United States)

    Castagna, Maura; Giuffra, Valentina; Fattori, Silvia; Vitiello, Angelica; Caramella, Davide; Giustini, Davide; Fornaciari, Gino

    2014-01-01

    Among the children found in the crypt of the Grand Duke Giangastone in S. Lorenzo Basilica (Florence), the skeletal remains of a 5-year-old child still wearing his fine high social status clothing were recovered. This child of the Medici family was identified as Don Filippino (1577-1582), son of the Grand Duke Francesco I (1541-1587) and Giovanna from Austria (1547 - 1578). The prince showed several pathological deformities of the cranial and post-cranial skeleton, including enlargement of the cranium, thinning of the cranial vault bones (craniotabes), platybasia and marked bending of femora, tibiae and fibulae. Differential diagnosis suggests that Don Filippino was affected by rickets. The occurrence of this metabolic disease related to vitamin D deficiency in a Renaissance high social class individual can be explained by the practice of very prolonged breast-feeding, up until two years of age. Maternal milk contains insufficient vitamin D ratios and retarded weaning severely exposes children to a higher risk of developing rickets, especially if dietary habits are combined with inadequate exposure to sunlight. Historical sources describe Don Filippino as frail and sickly, with frequent illnesses and persistent slight fevers, and it can be supposed that the child was frequently confined indoors, especially in the cold season. Integration of osteoarchaeological evidence with historical documentation suggests that bone lesions observed in the skeletal remains of Don Filippino are compatible with a diagnosis of rickets, caused by the custom of prolonged breast-feeding associated with inadequate sunlight exposure to sunlight. Historical sources describe Don Filippino as frail and sickly, with frequent illnesses and persistent slight fevers, and it can be supposed that the child was frequently confined indoors, especially in the cold season. Integration of osteoarchaeological evidence with historical documentation suggests that bone lesions observed in the skeletal

  8. The discovery of cometary activity in near-Earth asteroid (3552) Don Quixote

    Energy Technology Data Exchange (ETDEWEB)

    Mommert, Michael; Harris, Alan W. [Institute of Planetary Research, German Aerospace Center (DLR), Rutherfordstr. 2, D-12489 Berlin (Germany); Hora, Joseph L.; Smith, Howard A. [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138-1516 (United States); Reach, William T. [Universities Space Research Association, Stratospheric Observatory for Infrared Astronomy, MS 232-11, NASA Ames Research Center, Moffett Field, CA 94035 (United States); Emery, Joshua P. [Department of Earth and Planetary Sciences, University of Tennessee, 1412 Circle Dr., Knoxville, TN 37996 (United States); Thomas, Cristina A. [NASA Postdoctoral Program Fellow, NASA Goddard Space Flight Center, 8800 Greenbelt Rd, Greenbelt, MD 20771 (United States); Mueller, Michael [SRON Netherlands Institute for Space Research, Postbus 800, 9700 AV Groningen (Netherlands); Cruikshank, Dale P. [NASA Ames Research Center, Moffett Field, CA 94035 (United States); Trilling, David E. [Department of Physics and Astronomy, Northern Arizona University, P.O. Box 6010, Flagstaff, AZ 86011 (United States); Delbo, Marco [UNS-CNRS-Observatoire de la Cote d' Azur, BP4229, F-06304 Nice Cedex 4 (France)

    2014-01-20

    The near-Earth object (NEO) population, which mainly consists of fragments from collisions between asteroids in the main asteroid belt, is thought to include contributions from short-period comets as well. One of the most promising NEO candidates for a cometary origin is near-Earth asteroid (3552) Don Quixote, which has never been reported to show activity. Here we present the discovery of cometary activity in Don Quixote based on thermal-infrared observations made with the Spitzer Space Telescope in its 3.6 and 4.5 μm bands. Our observations clearly show the presence of a coma and a tail in the 4.5 μm but not in the 3.6 μm band, which is consistent with molecular band emission from CO{sub 2}. Thermal modeling of the combined photometric data on Don Quixote reveals a diameter of 18.4{sub −0.4}{sup +0.3} km and an albedo of 0.03{sub −0.01}{sup +0.02}, which confirms Don Quixote to be the third-largest known NEO. We derive an upper limit on the dust production rate of 1.9 kg s{sup –1} and derive a CO{sub 2} gas production rate of (1.1 ± 0.1) × 10{sup 26} molecules s{sup –1}. Spitzer Infrared Spectrograph spectroscopic observations indicate the presence of fine-grained silicates, perhaps pyroxene rich, on the surface of Don Quixote. Our discovery suggests that CO{sub 2} can be present in near-Earth space over a long time. The presence of CO{sub 2} might also explain that Don Quixote's cometary nature remained hidden for nearly three decades.

  9. 13 CFR 123.510 - What if you don't use your Military Reservist EIDL funds as authorized?

    Science.gov (United States)

    2010-01-01

    ... 13 Business Credit and Assistance 1 2010-01-01 2010-01-01 false What if you don't use your Military Reservist EIDL funds as authorized? 123.510 Section 123.510 Business Credit and Assistance SMALL... What if you don't use your Military Reservist EIDL funds as authorized? If your small business does...

  10. La reciprocidad y el don no son la misma cosa

    Directory of Open Access Journals (Sweden)

    Ricardo Gabriel Abduca

    2007-12-01

    Full Text Available El autor se propone mostrar por qué la noción de reciprocidad, aunque ha sido muchas veces tomada como sinónimo del fenómeno de intercambios de dones, corresponde a diversas situaciones sociales, por lo cual tiene poca utilidad descriptiva y explicativa. Examina algunos hitos de la historia antropológica, mostrando que más que un concepto, 'reciprocidad' es un palimpsesto sobre el cual, de Durkheim a Polanyi, se han ido escribiendo variadas nociones. En cambio, sugiere una definición de don más estricta, cercana a las ideas de Mauss y acorde con los avances de la etnografía y la teoría contemporánea. Sugiere distinguir entre circuitos cerrados de dones, donde circulan bienes de consumo final, y circuitos abiertos, donde los dones que circulan condensan el conjunto de las relaciones sociales.O objetivo deste texto é mostrar que a expressão 'reciprocidade', ainda foi considerada como sinônimo de 'câmbio de domes', é outra coisa. O autor sugere um breve esquema de alguns marcos do desenvolvimento da noção de 'reciprocidade' em a teoria antropológica, mostrando que o sentido dela expressão foi mudando até assemelhar um palimpsesto com múltiplas rescritas, no processo que vá de Durkheim a Polanyi. Defende um conceito de dom próximo às idéias de Mauss e a certos avances dela etnografia contemporânea e a teoria social. Distingue deus tipos de circuitos de circulação de domes: fechados, onde circulam bens de consumação direita, e apertos, onde circulam certo tipo de bens que condensam o conjunto das relações sociais.The aim of this text is to show how the notion of 'reciprocity', although have been considered a synonymous of gift exchange, veils different social situations. A brief survey of the history of the use of the word shows how 'reciprocity', from Durkheim to Polanyi, seems as a palimpsest of various notions. Although, he essays a defense of a concept of 'gift' related to the Maussian texts, and closer to some

  11. Un curioso coleccionista del siglo XVII: don Manuel de Porras

    Directory of Open Access Journals (Sweden)

    Agulló y Cobo, Mercedes

    2003-09-01

    Full Text Available Art collecting - an avocation almost exclusive to royalty in Spain - has not had representatives among those who did not belong to the high nobility, not even among artists themselves. In many cases, the word "collection" has been applied to what in reality were mere lists of patrimonial properties. For all of the above, it is especially noteworthy to encounter someone who neither belonged to an elevated class nor was a painter, but who managed to unite, at great personal sacrifice, an important art collection. Manuel de Porras owned interesting and a relatively large number of paintings, sculptures and prints, some of them extremely valuable. As a good collector, in order to avoid the dispersion of these works, he donated them to the Shod Trinitarians in Madrid and the Oratory of Saint Philip Neri in Alcalá, but unfortunately, in spite of his good intentions, nothing has survived.

    El coleccionismo —afición casi exclusiva de Reyes en España— no ha tenido representantes entre personas que no pertenecieran a la alta nobleza, ni entre los propios artistas. En muchos casos lo que se ha venido llamando colecciones son sólo relación de bienes patrimoniales. Por ello, resulta más llamativo encontrar alguien que, sin pertenecer a una clase elevada ni ser artista, lograra reunir —aun a costa de grandes sacrificios que le llevaron al extremo de ser enterrado de limosna— una tan importante colección de obras de arte como el personaje objeto de este artículo: Manuel de Porras, quien contaba con un interesante y elevado (para un particular sin títulos número de pinturas y esculturas, y un importantísimo número de dibujos y estampas, algunas valiosísimas y de alto precio. Desgraciadamente, aunque lo donó —como buen coleccionista, para evitar su dispersión— a los Trinitarios calzados de Madrid y al Oratorio de San Felipe Neri de Alcalá, nada de ello ha llegado a nuestros días.

  12. PERKEMBANGAN LATISIFER PADA KULTUR KALUS CATHARANTHUS ROSEUS (L G. DON YANG DIINDUKSI DENGAN KOMBINASI ZAT PENGATUR TUMBUH KINETIN + NAA

    Directory of Open Access Journals (Sweden)

    NI NYOMAN DARSINI

    2011-12-01

    Full Text Available The development of laticifer on callus culture of Catharanthus roseus (L G Don in Zenk medium supplemented with combination of plant growth regulator kinetin + NAA was studied. The explants were taken from the second folium from shoot apex. Development of laticifer was observed using descriptive analysis method for callus anatomy and percentage of laticifer was observed during 4–14 weeks of callus development. The percentage of laticifer was determined by counting the average number of the laticifer and the average number of surrounding cells in every optical field of few under light microscope. The results showed that early development of laticifer which was induced with plant regulator growth kinetin + NAA was found in the 9 weeks old callus. The laticifer has specific characteristics i.e. thicker cell wall and longer cell than sorounding cell. Elongated laticifer was observed at 12 weeks old callus. The highest percentage of laticifer on callus C. roseus induced with combination of kinetin and NAA was found in 12 weeks old callus i.e. 0,12%. At 13 and 14 weeks old callus, the anatomy of laticifer was similar to that at 12 weeks old callus, but the percentage was lower.

  13. Dedicated to the 100th anniversary of Don Higher Medical Education

    Directory of Open Access Journals (Sweden)

    V. P. Terentev

    2015-01-01

    Full Text Available This year the medical community of the Don marks 85th anniversary since the founding of the Rostov Regional Scientific Medical Society of Physicians. At the root of a society were outstanding clinicians, who left a bright trace in the history of Don Medicine — Professors Zavadsky I.V. , Kastanayan E.M. and others.The merits of the Rostov Regional Scientific Medical Society of Physicians are hard to overestimate, and today one of our most important tasks is to ensure the continuous education of doctors in the region.

  14. Interbasin isotopic correspondence between upper-ocean bulk DON and subsurface nitrate and its implications for marine nitrogen cycling

    Science.gov (United States)

    Knapp, Angela N.; Sigman, Daniel M.; Lipschultz, Fred; Kustka, Adam B.; Capone, Douglas G.

    2011-12-01

    Measurements to date have shown that both bulk and high molecular weight marine dissolved organic nitrogen (DON) have a 15N/14N that is substantially higher than the 15N/14N of suspended particulate organic nitrogen (PNsusp) found in the same surface waters (with δ15N of ˜4 to 5‰ and ˜-1 to 1‰, respectively). Moreover, the concentration and 15N/14N of DON are much less dynamic than those of PNsusp. These observations raise questions regarding the role of DON in the upper ocean nitrogen (N) cycle. In this study, the concentration and 15N/14N of nitrate and DON was measured in the upper 300 m of the oligotrophic North Atlantic and North Pacific Oceans. Comparing these two regions, the average DON concentration in the upper 100 m is similar, between 4.5 and 5.0 μM, but the average δ15N of DON is significantly different, 3.9‰ versus air in the North Atlantic and 4.7‰ in the North Pacific. This difference parallels a similar isotopic difference between shallow nitrate in these two regions; at 200 m in the North Atlantic, the δ15N of nitrate is 2.6‰, while it is 4.0‰ in the North Pacific. This isotopic correlation between surface DON and subsurface nitrate indicates that DON is actively participating in the upper ocean N cycle of each region. We describe a conceptual model that explains the elevation of the 15N/14N of DON relative to surface ocean PNsusp as well as the interbasin difference in the 15N/14N of DON. In this model, DON is produced from PNsusp without isotopic fractionation but DON is removed by fractionating processes. The ammonium and simple organic N compounds released by DON decomposition reactions are reassimilated by algae into the PNsusp pool, as an integral part of the ammonium-centered cycle that lowers the 15N/14N of PNsusp relative to the nitrate supply from below. This interpretation is consistent with the understanding of the chemical controls on isotope fractionation and is analogous to the previously posed explanation for the

  15. Picrasma quassiodes (D. Don) Benn. attenuates lipopolysaccharide (LPS)-induced acute lung injury.

    Science.gov (United States)

    Lee, Jae-Won; Park, Ji-Won; Shin, Na-Rae; Park, So-Yeon; Kwon, Ok-Kyoung; Park, Hyun Ah; Lim, Yourim; Ryu, Hyung Won; Yuk, Heung Joo; Kim, Jung Hee; Oh, Sei-Ryang; Ahn, Kyung-Seop

    2016-09-01

    Picrasma quassiodes (D.Don) Benn. (PQ) is a medicinal herb belonging to the family Simaroubaceae and is used as a traditional herbal remedy for various diseases. In this study, we evaluated the effects of PQ on airway inflammation using a mouse model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) and LPS-stimulated raw 264.7 cells. ALI was induced in C57BL/6 mice by the intranasal administration of LPS, and PQ was administered orally 3 days prior to exposure to LPS. Treatment with PQ significantly attenuated the infiltration of inflammatory cells in the bronchoalveolar lavage fluid (BALF). PQ also decreased the production of reactive oxygen species (ROS) and pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6 in BALF. In addition, PQ inhibited airway inflammation by reducing the expression of inducible nitric oxide synthase (iNOS) and by increasing the expression of heme oxygenase-1 (HO-1) in the lungs. Furthermore, we demonstrated that PQ blocked the activation of mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) in the lungs of mice with LPS-induced ALI. In the LPS-stimulated RAW 264.7 cells, PQ inhibited the release of pro-inflammatory cytokines and increased the mRNA expression of monocyte chemoattractant protein-1 (MCP-1). Treatment with PQ decreased the translocation of nuclear factor (NF)-κB to the nucleus, and increased the nuclear translocation of nuclear factor erythroid-2-related factor 2 (Nrf2) and the expression of HO-1. PQ also inhibited the activation of p38 in the LPS-stimulated RAW 264.7 cells. Taken together, our findings demonstrate that PQ exerts anti-inflammatory effects against LPS-induced ALI, and that these effects are associated with the modulation of iNOS, HO-1, NF-κB and MAPK signaling. Therefore, we suggest that PQ has therapeutic potential for use in the treatment of ALI. PMID:27431288

  16. Toward Complete Inclusion: Lesbian, Gay, Bisexual, and Transgender Military Service Members after Repeal of Don't Ask, Don't Tell.

    Science.gov (United States)

    Aford, Brandon; Lee, Shawna J

    2016-07-01

    The 2010 repeal of Don't Ask, Don't Tell (DADT) is one example of how U.S. public policy has shifted toward greater inclusion of lesbian, gay, and bisexual (LGB) individuals. The repeal of DADT reversed the practice of discharging LGB service members on the basis of sexual identity. LGB service members may now serve their country without fear of direct repercussions stemming from sexual identity. Though it is a statutory step toward parity, DADT repeal does not address a number of cultural and institutional inequities that continue to hinder full inclusion of sexual minority service members. Notably, as discussed in this article, DADT largely ignores issues facing the transgender population. This study examines remaining inequities and their ramifications for lesbian, gay, bisexual, and transgender service members and their families. The article concludes with practice and policy recommendations for culturally competent social work practice with military service members across the sexual identity spectrum.

  17. Toward Complete Inclusion: Lesbian, Gay, Bisexual, and Transgender Military Service Members after Repeal of Don't Ask, Don't Tell.

    Science.gov (United States)

    Aford, Brandon; Lee, Shawna J

    2016-07-01

    The 2010 repeal of Don't Ask, Don't Tell (DADT) is one example of how U.S. public policy has shifted toward greater inclusion of lesbian, gay, and bisexual (LGB) individuals. The repeal of DADT reversed the practice of discharging LGB service members on the basis of sexual identity. LGB service members may now serve their country without fear of direct repercussions stemming from sexual identity. Though it is a statutory step toward parity, DADT repeal does not address a number of cultural and institutional inequities that continue to hinder full inclusion of sexual minority service members. Notably, as discussed in this article, DADT largely ignores issues facing the transgender population. This study examines remaining inequities and their ramifications for lesbian, gay, bisexual, and transgender service members and their families. The article concludes with practice and policy recommendations for culturally competent social work practice with military service members across the sexual identity spectrum. PMID:27501643

  18. Organ Damage and Hepatic Lipid Accumulation in Carp (Cyprinus carpio L. after Feed-Borne Exposure to the Mycotoxin, Deoxynivalenol (DON

    Directory of Open Access Journals (Sweden)

    Constanze Pietsch

    2014-02-01

    Full Text Available Deoxynivalenol (DON frequently contaminates animal feed, including fish feed used in aquaculture. This study intends to further investigate the effects of DON on carp (Cyprinus carpio L. at concentrations representative for commercial fish feeds. Experimental feeding with 352, 619 or 953 μg DON kg−1 feed resulted in unaltered growth performance of fish during six weeks of experimentation, but increased lipid peroxidation was observed in liver, head kidney and spleen after feeding of fish with the highest DON concentration. These effects of DON were mostly reversible by two weeks of feeding the uncontaminated control diet. Histopathological scoring revealed increased liver damage in DON-treated fish, which persisted even after the recovery phase. At the highest DON concentration, significantly more fat, and consequently, increased energy content, was found in whole fish body homogenates. This suggests that DON affects nutrient metabolism in carp. Changes of lactate dehydrogenase (LDH activity in kidneys and muscle and high lactate levels in serum indicate an effect of DON on anaerobic metabolism. Serum albumin was reduced by feeding the medium and a high dosage of DON, probably due to the ribotoxic action of DON. Thus, the present study provides evidence of the effects of DON on liver function and metabolism.

  19. The Postmodern Don Juan Scoring on the Internet. Representation of Gender and Culture of Late Capitalism in the Film Don Jon (2013)

    OpenAIRE

    Sóley Arngrímsdóttir 1990

    2015-01-01

    This essay explores how the film Don Jon (2013) is an astute criticism on modern society, showing how gender inequality is embedded in media narratives and ultimately institutionalized in pornography. Reading the film through literary theories of the postmodern, such as Frederic Jameson, Jean Baudrillard, Laura Mulvey and Judith Butler, the essay demonstrates how the setting and gender relations represented in the film exemplify the culture of late capitalism, with its fragmented sense of his...

  20. Deoxynivalenol induced mouse skin cell proliferation and inflammation via MAPK pathway

    International Nuclear Information System (INIS)

    Several toxicological manifestations of deoxynivalenol (DON), a mycotoxin, are well documented; however, dermal toxicity is not yet explored. The effect of topical application of DON to mice was studied using markers of skin proliferation, inflammation and tumor promotion. Single topical application of DON (84–672 nmol/mouse) significantly enhanced dermal hyperplasia and skin edema. DON (336 and 672 nmol) caused significant enhancement in [3H]-thymidine uptake in DNA along with increased myeloperoxidase and ornithine decarboxylase activities, suggesting tissue inflammation and cell proliferation. Furthermore, DON (168 nmol) caused enhanced expression of RAS, and phosphorylation of PI3K/Akt, ERK, JNK and p38 MAPKs. DON exposure also showed activation of transcription factors, c-fos, c-jun and NF-κB along with phosphorylation of IkBα. Enhanced phosphorylation of NF-κB by DON caused over expression of target proteins, COX-2, cyclin D1 and iNOS in skin. Though a single topical application of DMBA followed by twice weekly application of DON (84 and 168 nmol) showed no tumorigenesis after 24 weeks, however, histopathological studies suggested hyperplasia of the epidermis and hypertrophy of hair follicles. Interestingly, intestine was also found to be affected as enlarged Peyer's patches were observed, suggesting inflammatory effects which were supported by elevation of inflammatory cytokines after 24 weeks of topical application of DON. These results suggest that DON induced cell proliferation in mouse skin is through the activation of MAPK signaling pathway involving transcription factors NFκB and AP-1, further leading to transcriptional activation of downstream target proteins c-fos, c-jun, cyclin D1, iNOS and COX-2 which might be responsible for its inflammatory potential. - Highlights: • Topical application of DON enhanced epidermal inflammation and cell proliferation. • DON follows PI3K/Akt/MAPK signaling cascade, with activation of AP-1 and NF-κB.

  1. Deoxynivalenol induced mouse skin cell proliferation and inflammation via MAPK pathway

    Energy Technology Data Exchange (ETDEWEB)

    Mishra, Sakshi [Food Drug and Chemical Toxicology, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), P.O. Box No. 80, Mahatma Gandhi Marg, Lucknow 226 001 (India); Department of Biochemistry, Banaras Hindu University (BHU), Varanasi (India); Tripathi, Anurag [Food Drug and Chemical Toxicology, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), P.O. Box No. 80, Mahatma Gandhi Marg, Lucknow 226 001 (India); Chaudhari, Bhushan P. [Pathology Laboratory, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Mahatma Gandhi Marg, PO Box 80, Lucknow 226001, Uttar Pradesh (India); Dwivedi, Premendra D. [Food Drug and Chemical Toxicology, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), P.O. Box No. 80, Mahatma Gandhi Marg, Lucknow 226 001 (India); Pandey, Haushila P. [Department of Biochemistry, Banaras Hindu University (BHU), Varanasi (India); Das, Mukul, E-mail: mditrc@rediffmail.com [Food Drug and Chemical Toxicology, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), P.O. Box No. 80, Mahatma Gandhi Marg, Lucknow 226 001 (India)

    2014-09-01

    Several toxicological manifestations of deoxynivalenol (DON), a mycotoxin, are well documented; however, dermal toxicity is not yet explored. The effect of topical application of DON to mice was studied using markers of skin proliferation, inflammation and tumor promotion. Single topical application of DON (84–672 nmol/mouse) significantly enhanced dermal hyperplasia and skin edema. DON (336 and 672 nmol) caused significant enhancement in [{sup 3}H]-thymidine uptake in DNA along with increased myeloperoxidase and ornithine decarboxylase activities, suggesting tissue inflammation and cell proliferation. Furthermore, DON (168 nmol) caused enhanced expression of RAS, and phosphorylation of PI3K/Akt, ERK, JNK and p38 MAPKs. DON exposure also showed activation of transcription factors, c-fos, c-jun and NF-κB along with phosphorylation of IkBα. Enhanced phosphorylation of NF-κB by DON caused over expression of target proteins, COX-2, cyclin D1 and iNOS in skin. Though a single topical application of DMBA followed by twice weekly application of DON (84 and 168 nmol) showed no tumorigenesis after 24 weeks, however, histopathological studies suggested hyperplasia of the epidermis and hypertrophy of hair follicles. Interestingly, intestine was also found to be affected as enlarged Peyer's patches were observed, suggesting inflammatory effects which were supported by elevation of inflammatory cytokines after 24 weeks of topical application of DON. These results suggest that DON induced cell proliferation in mouse skin is through the activation of MAPK signaling pathway involving transcription factors NFκB and AP-1, further leading to transcriptional activation of downstream target proteins c-fos, c-jun, cyclin D1, iNOS and COX-2 which might be responsible for its inflammatory potential. - Highlights: • Topical application of DON enhanced epidermal inflammation and cell proliferation. • DON follows PI3K/Akt/MAPK signaling cascade, with activation of AP-1 and NF

  2. Biologists napping while work militarized.

    Science.gov (United States)

    Dando, Malcolm

    2009-08-20

    As researchers discover more agents that alter mental states, the Chemical Weapons Convention needs modification to help ensure that the life sciences are not used for hostile purposes, says Malcolm Dando.

  3. Training Experimental Biologists in Bioinformatics

    Directory of Open Access Journals (Sweden)

    Pedro Fernandes

    2012-01-01

    Full Text Available Bioinformatics, for its very nature, is devoted to a set of targets that constantly evolve. Training is probably the best response to the constant need for the acquisition of bioinformatics skills. It is interesting to assess the effects of training in the different sets of researchers that make use of it. While training bench experimentalists in the life sciences, we have observed instances of changes in their attitudes in research that, if well exploited, can have beneficial impacts in the dialogue with professional bioinformaticians and influence the conduction of the research itself.

  4. Genomic toolboxes for conservation biologists

    OpenAIRE

    Angeloni, F.; Wagemaker, N.; Vergeer, P.; Ouborg, N.J.

    2011-01-01

    Conservation genetics is expanding its research horizon with a genomic approach, by incorporating the modern techniques of next-generation sequencing (NGS). Application of NGS overcomes many limitations of conservation genetics. First, NGS allows for genome-wide screening of markers, which may lead to a more representative estimation of genetic variation within and between populations. Second, NGS allows for distinction between neutral and non-neutral markers. By screening populations on thou...

  5. NMR for chemists and biologists

    CERN Document Server

    Carbajo, Rodrigo J

    2013-01-01

    This book intends to be an easy and concise introduction to the field of nuclear magnetic resonance or NMR, which has revolutionized life sciences in the last twenty years. A significant part of the progress observed in scientific areas like Chemistry, Biology or Medicine can be ascribed to the development experienced by NMR in recent times. Many of the books currently available on NMR deal with the theoretical basis and some of its main applications, but they generally demand a strong background in Physics and Mathematics for a full understanding. This book is aimed to a wide scientific audie

  6. Reproducible Bioinformatics Research for Biologists

    Science.gov (United States)

    This book chapter describes the current Big Data problem in Bioinformatics and the resulting issues with performing reproducible computational research. The core of the chapter provides guidelines and summaries of current tools/techniques that a noncomputational researcher would need to learn to pe...

  7. Studies on cytological, physiological and genetic characteristics in somatic mutant strains of Sugi (Cryptomeria japonica D. Don)

    International Nuclear Information System (INIS)

    From microscopic observation of the pollen of induced mutant strains in Sugi (Cryptomeria japonica D. Don), it was found that there were large differences in pollen fertility among the mutant strains, and that it deviated year to year from the mother plants. The large differences in frequency of sterile pollen among mutant strains depended on the genetic characteristics of each mutant strain. Higher frequencies of sterile pollen were observed at the terminal part of branchlets in some mutant strains, and this was considered to be induced by the lateness of flower-bud formation at low temperature conditions in late summer. Delayed formation and gibberellic acid treatment applied for flower induction resulted in low fertility and abnormality of pollen in mutant strains. Chromosome aberration in mutant strains was caused either by gamma irradiation or by some mutational events that responded to environmental conditions. In the former case, aberration might have been maintained for a long period through vegetative propagation. Some of the irregularities were due to mitotic cell division, because cells with micronuclei at the pacytene stage in pollen mother cells and with fragments at MI were observed. Somatic mutability of Kuma-sugi mutants after re-irradiation was investigated. From waxless mutants morphological somatic mutations, which have fat or stout stems and thick and short needles, were frequently produced, whereas from morphological mutants the lowest somatic mutation frequency was induced. In some mutant strains higher rooting ability than the mother plants was found, and the possibility of character improvement was pointed out. (author)

  8. Subaquatic soils in the Volga, Don and Kuban Rivers deltas.

    Science.gov (United States)

    Tkachenko, Anna; Gerasimova, Maria; Lychagin, Mikhail

    2015-04-01

    River deltas occupy a special interface position in the environment and are characterized by contrasting hydrological and landscape-geochemical regimes. Small depth of water and weak currents contribute to suspended matter deposition. Significant spread of aquatic plants provides the enrichment of subaquatic soils in organic matter. All these factors contribute to the formation of different subaquatic soils. Possibility of including them in the classification systems is discussed by many authors (Demas and Rabenhorst, 2001; Stolt et al., 2011); there is also a special subaquatic qualifier for submerged soils in WRB; however, they are still absent in many national classification systems, as well as in the recent Russian one (2008). The purpose of this research is to reveal the properties of the subaquatic soils in the Volga, Don and Kuban Rivers deltaic areas and to propose pedogenetic approaches to categorize AQUAZEMS. Investigations of deltaic areas were performed in 2010-2012 in deltaic lagoons, fresh-water bays, small channels, oxbow lakes, and also in the part of deltaic near-shore zone. Morphological descriptions of distinguishable layers (colour, texture, thickness, boundaries, consistence, plant residues and shell debris) were made in columns obtained by augering as it is done by other researchers (Stolt et al., 2011), and supplemented with analytical data (pH, Eh, TDS, particle-size composition, and Corg). It is suggested to name the horizons in aquazems in the same way as in terrestrial soils in the recent Russian soil classification system, and apply symbols starting with the combination of caps - AQ. Most typical for aquazems is their aquagley AQG horizon that has features similar to terrestrial gleys - homogeneity in color and consistence, permeation by clay, predominance of dove grey colour. The AQG horizon gradually merges into parent material - stratified bottom sediments. The "topsoil" is usually enriched in organic matter and may be different in

  9. A new phenylethanoid glycoside from Rabdosia lophanthoides (Buch.-Ham.ex D.Don)Hara.

    Institute of Scientific and Technical Information of China (English)

    Wei Sheng Feng; Xin Yu Zang; Xiao Ke Zheng; Yan Zhi Wang

    2009-01-01

    A new phenylethanoid glycoside,3-hydroxy-4-methoxy-β-phenylethoxy-P-α-L-rhamnopyranosyl-(1-3)-2-O-acetyl-O-β-D-glucopyranoside,named Iophanthoside A,was isolated from Rabdosia lophanthoides(Buch.-Ham.ex D.Don)Hara.Its structurewas determined by spectroscopic evidences.

  10. Encouraging and Clarifying "Don't Know" Responses Enhances Interview Quality

    Science.gov (United States)

    Scoboria, Alan; Fisico, Stephanie

    2013-01-01

    Investigative interviewers seek to obtain complete and accurate accounts of events from witnesses. Two studies examined the influence of instructions about the use of don't know (DK) responses and of clarifying the meanings of DK responses on the quality of responding to questioning. Participants watched a video, and after a delay (Study 1, 30…

  11. Magnetic observations of pulsating B and Be stars with ESPaDOnS and Narval

    NARCIS (Netherlands)

    J. Silvester; C. Neiner; H.F. Henrichs; G.A. Wade; E. Alecian; V. Petit

    2008-01-01

    Discoveries of magnetic fields in pulsating B and Be stars have been claimed from low-resolution spectropolarimetric observations with FORS1 at VLT. We used the new generation of high-resolution spectropolarimeters, ESPaDOnS at CFHT and NARVAL at TBL, to check for the existence of these fields. We f

  12. Rap Music in School Counseling Based on Don Elligan's Rap Therapy

    Science.gov (United States)

    Gonzalez, Tiphanie; Hayes, B. Grant

    2009-01-01

    In 2000, Don Elligan introduced Rap Therapy as a psychotherapeutic intervention for working with at-risk youths, primarily African American males whose identities were highly influenced by rap music. Rap music can engage a population of youth who often enter counseling apprehensively (Elligan 2000, 2004; Tillie-Allen, 2005). This article reviews…

  13. "They Don't Know Anything!": Latinx Immigrant Students Appropriating the Oppressor's Voice

    Science.gov (United States)

    Monzó, Lilia D.

    2016-01-01

    This article discusses internalized oppression among Latinx communities through a revolutionary critical pedagogy. Data from a two-year ethnography of Latinx immigrant families show that students were developing deficit perspectives toward their parents, claiming that "they don't know anything," based on their positioning as powerless…

  14. "Boys Don't Cry," a Movie Review: Implications for Counseling Persons Who Are Transgendered

    Science.gov (United States)

    Aguilar, Jinnelle Veronique

    2010-01-01

    People who fall under the umbrella of transgender belong to one of the most marginalized groups within society. Many who do not pass within the gender binary categories imposed by social norms experience discrimination and marginalization. In this article, the author discusses the film "Boys Don't Cry" and introduces the reader to the varieties of…

  15. 33 CFR 208.82 - Hetch Hetchy, Cherry Valley, and Don Pedro Dams and Reservoirs.

    Science.gov (United States)

    2010-07-01

    ... flood control all as follows: (a) Storage space in Don Pedro Reservoir shall be kept available for flood... this section. The Flood-Control Storage Reservoir Diagram in force as of the promulgation of this... County of San Francisco, California. (d) Any water temporarily stored in the flood-control...

  16. The Don Bosco Way to Train Disadvantaged Youth. Discussion Paper No. 92.

    Science.gov (United States)

    Mashek, R. W.

    Founded by Don Bosco in 1859 in Turin, Italy, the Salesian Society helps disadvantaged youth in over 100 countries, according to 1991 data. Salesians operate technical training institutes and run numerous informal shops and training courses within their schools, orphanages, street children shelters, and youth centers. The institutes are financed…

  17. Why I don't kill myself : [poems] / Paul-Eerik Rummo

    Index Scriptorium Estoniae

    Rummo, Paul-Eerik, 1942-

    2003-01-01

    Autori lühitutvustus lk. 262. Sisu: Why I don't kill myself ; The sky stoops over the earth ; Clinging ; Crooning. Orig.: Miks ma end ära ei tapa ; "Taevas on kummargil üle maa..." ; Kinni hoidmas ; Poolüminal

  18. 2003:做与不做%Do and Don't in 2003

    Institute of Scientific and Technical Information of China (English)

    张强

    2003-01-01

    @@ DON'T: Keep worshipping former idols. Sure, they' re making a movie with Kelly Clarkson and Justin Guarini, but by the time it hits theaters, you're gonna be swooning(惊讶, 陶醉) over some new pixie-esque Mariah wanna be.

  19. Therapists' continuations following I don't know-responses of adolescents in psychotherapy

    NARCIS (Netherlands)

    Jager, Margot; Huiskes, Mike; Metselaar, Janneke; Knorth, Erik J; De Winter, Andrea F; Reijneveld, Sijmen A

    2016-01-01

    OBJECTIVE: In psychotherapy clients' I don't know-responses (IDK-responses) to therapists' questions are typically considered to be non-cooperating behaviors. How therapists actually handle these behaviors remains unclear. This study therefore aims to assess client-therapist interactions following I

  20. Have a Baby or Young Child With a Cold? Most Don't Need Medicines

    Science.gov (United States)

    ... For Consumers Home For Consumers Consumer Updates Have a Baby or Young Child With a Cold? Most Don't Need Medicines Share Tweet ... the throat. back to top When to Call a Doctor Not every sniffle or cough merits a ...

  1. Etude des variations de la teneur en isoflavones et de leur composition dans le germe et le cotylédon de la graine de soja [Glycine max (L.) Merrill

    OpenAIRE

    Rasolohery, Claudine Aimée

    2007-01-01

    La graine de soja est très riche en isoflavones, molécules réparties entre les cotylédons et le germe. Ce travail porte sur l'étude de la variabilité et de la cinétique d'accumulation des isoflavones dans ces deux fractions de la graine. Les isoflavones du germe (daidzéine et glycitéine) ont une concentration 5 à 10 fois supérieure à celle des cotylédons (composés de génistéine et daidzéine). Alors que les pourcentages des différentes isoflavones dans les deux compartiments sont principalemen...

  2. 让英语课堂沐浴德育的春风——Unit12 Don't eat in class教学设计

    Institute of Scientific and Technical Information of China (English)

    崔丽娟

    2010-01-01

    @@ 一、教学目标 1.知识目标 本节课学生掌握下列新单词和句型: rule,hallway,fight,classroom,Ms,outside,dining hall,have to,School RuleDon't arrive late for class.Don't fight.Don't run in the hallway.Don't eat in the classroom.Don't listen to music in the classroom.What are the rules?We can't arrive late for class.

  3. WImpiBLAST: web interface for mpiBLAST to help biologists perform large-scale annotation using high performance computing.

    Science.gov (United States)

    Sharma, Parichit; Mantri, Shrikant S

    2014-01-01

    The function of a newly sequenced gene can be discovered by determining its sequence homology with known proteins. BLAST is the most extensively used sequence analysis program for sequence similarity search in large databases of sequences. With the advent of next generation sequencing technologies it has now become possible to study genes and their expression at a genome-wide scale through RNA-seq and metagenome sequencing experiments. Functional annotation of all the genes is done by sequence similarity search against multiple protein databases. This annotation task is computationally very intensive and can take days to obtain complete results. The program mpiBLAST, an open-source parallelization of BLAST that achieves superlinear speedup, can be used to accelerate large-scale annotation by using supercomputers and high performance computing (HPC) clusters. Although many parallel bioinformatics applications using the Message Passing Interface (MPI) are available in the public domain, researchers are reluctant to use them due to lack of expertise in the Linux command line and relevant programming experience. With these limitations, it becomes difficult for biologists to use mpiBLAST for accelerating annotation. No web interface is available in the open-source domain for mpiBLAST. We have developed WImpiBLAST, a user-friendly open-source web interface for parallel BLAST searches. It is implemented in Struts 1.3 using a Java backbone and runs atop the open-source Apache Tomcat Server. WImpiBLAST supports script creation and job submission features and also provides a robust job management interface for system administrators. It combines script creation and modification features with job monitoring and management through the Torque resource manager on a Linux-based HPC cluster. Use case information highlights the acceleration of annotation analysis achieved by using WImpiBLAST. Here, we describe the WImpiBLAST web interface features and architecture, explain design

  4. WImpiBLAST: web interface for mpiBLAST to help biologists perform large-scale annotation using high performance computing.

    Directory of Open Access Journals (Sweden)

    Parichit Sharma

    Full Text Available The function of a newly sequenced gene can be discovered by determining its sequence homology with known proteins. BLAST is the most extensively used sequence analysis program for sequence similarity search in large databases of sequences. With the advent of next generation sequencing technologies it has now become possible to study genes and their expression at a genome-wide scale through RNA-seq and metagenome sequencing experiments. Functional annotation of all the genes is done by sequence similarity search against multiple protein databases. This annotation task is computationally very intensive and can take days to obtain complete results. The program mpiBLAST, an open-source parallelization of BLAST that achieves superlinear speedup, can be used to accelerate large-scale annotation by using supercomputers and high performance computing (HPC clusters. Although many parallel bioinformatics applications using the Message Passing Interface (MPI are available in the public domain, researchers are reluctant to use them due to lack of expertise in the Linux command line and relevant programming experience. With these limitations, it becomes difficult for biologists to use mpiBLAST for accelerating annotation. No web interface is available in the open-source domain for mpiBLAST. We have developed WImpiBLAST, a user-friendly open-source web interface for parallel BLAST searches. It is implemented in Struts 1.3 using a Java backbone and runs atop the open-source Apache Tomcat Server. WImpiBLAST supports script creation and job submission features and also provides a robust job management interface for system administrators. It combines script creation and modification features with job monitoring and management through the Torque resource manager on a Linux-based HPC cluster. Use case information highlights the acceleration of annotation analysis achieved by using WImpiBLAST. Here, we describe the WImpiBLAST web interface features and architecture

  5. Terrorism, Media, and the Ethics of Fiction: Transatlantic Perspectives on Don DeLillo.Eds.Peter Schneck and Philipp Schweighauser.

    Directory of Open Access Journals (Sweden)

    Angeliki Tseti

    2011-10-01

    Full Text Available Far from being yet another collection of critical essays in a long array of references on Don DeLillo, Terrorism, Media and the Ethics of Fiction is a valuable addition to the already extensive bibliography on one of the most prolific writers of our times. What differentiates this book from previous collections is its premise, already indicated in the subtitle: Transatlantic Perspectives on Don DeLillo. Indeed, as editors Schneck and Schweighauser state in their introduction “Don DeLillo’s wo...

  6. The greatest biologist in 20th century—Francis Crick%20世纪最伟大的生物学家——弗朗西斯?克里克

    Institute of Scientific and Technical Information of China (English)

    顾凡及

    2016-01-01

    A biography of UK scientist Francis Crick who is called as the greatest biologist in 20th century is presented in this article. His contributions to life sciences and his approaches to scholarship are also given.%介绍了被称为"20世纪最伟大的生物学家"的英国科学家克里克的生平、对生命科学的贡献和治学之道.

  7. 20世纪最伟大的生物学家——弗朗西斯?克里克%The greatest biologist in 20th century—Francis Crick

    Institute of Scientific and Technical Information of China (English)

    顾凡及

    2016-01-01

    介绍了被称为"20世纪最伟大的生物学家"的英国科学家克里克的生平、对生命科学的贡献和治学之道.%A biography of UK scientist Francis Crick who is called as the greatest biologist in 20th century is presented in this article. His contributions to life sciences and his approaches to scholarship are also given.

  8. Role of cancer stem cells in hepatocarcinogenesis

    OpenAIRE

    Wang, Bo; Jacob, Samson T.

    2011-01-01

    There has been considerable interest in cancer stem cells (CSCs) among cancer biologists and clinicians, most likely because of their role in the heterogeneity of cancer and their potential application in cancer therapeutics. Recent studies suggest that CSCs play a key role in liver carcinogenesis. A small subpopulation of cancer cells with CSC properties has been identified and characterized from hepatocellular carcinoma (HCC) cell lines, animal models and human primary HCCs. Considering the...

  9. Formation of algae-derived DON and characterization of algae organic matter (AOM) from different stages%藻源型溶解性有机氮的产生及不同时期藻类有机物的特性

    Institute of Scientific and Technical Information of China (English)

    古励; 郭显强; 丁昌龙; 何强; 单志俊

    2015-01-01

    Formation rules of dissolved organic nitrogen (DON), as well as characteristics of extracellular oganic matter (EOM) & intracellular organic matter (IOM) during the growth ofMicrocystis aeruginous have been studied. The results show as follows: DON concentration of EOM shows a growing tendency during the growth ofMicrocystis aeruginous, the yield of which is less than 0.05mg DON per 107cells in the early growth phase, around 0.40mg DON per 107cells in the late growth phase, respectively; molecular weight distributions of EOM during exponential phase and decline phase mainly concentrate in range of less than 1kDa and more than 100kDa, while organic matter less than 30kDa dominating EOM in stationary phase;molecular weight distributions of IOM concentrate in range of more than 1kDa and less than 100kDa in the three phases. The main fluorescence regions of EOM occur at the fluorescence region of humic acid-like materials and soluble microbial products. By contrast, the region of aromatic proteins and soluble microbial products dominate the main region of EEM spectra of IOM.%研究了铜绿微囊藻藻细胞全生命周期中氮的转化过程以及藻类有机物的性质,结果表明:溶解性有机氮(DON)在藻类生长周期中总体呈现上升趋势,藻细胞在其生长初期释放量小于 0.05mgDON/107cells,衰亡期释放能力约为 0.40mgDON/107cells;胞外有机物(EOM)中分子量分布在对数期和衰亡期以100kDa 为主,稳定期以100kDa范围内.三维荧光光谱分析表明,EOM中荧光物质主要为类腐植酸和溶解性微生物代谢产物;而IOM主要由类芳香蛋白和溶解性微生物代谢产物组成.

  10. Penetration of fosfomycin into IPEC-J2 cells in the presence or absence of deoxynivalenol.

    Directory of Open Access Journals (Sweden)

    Guadalupe Martínez

    Full Text Available Fosfomycin (FOS is an antibiotic used in pig farms for treatment and prevention of infections caused by resistant bacteria during the post-weaning period. Antibiotics and non-toxic concentrations of mycotoxins, such as deoxynivalenol (DON are frequently found in the diet of animals. These compounds can establish interactions in the intestinal tract, which could affect and/or modify the penetration of FOS to enterocytes. The aim of this study was to determine the penetration of FOS into IPECJ-2 cells, a cell line derived from the small intestine of piglets, in the presence and absence of DON. The results from this study showed that there was statistically significant difference in the intracellular concentration of FOS between cells incubated with 580 µg/ml FOS and cells incubated with 580 µg/ml FOS and 1 µg/ml DON. The Cmax of the intracellular antibiotic in the culture plates incubated with FOS in absence of DON was 45.81 µg/ml with a tmax of 4 h. When IPEC-2 cells were incubated with FOS and DON the Cmax was 20.06 µg/ml and the tmax was 30 min. It is concluded that the non-toxic concentration of DON on IPEC-J2 cells after short-term exposure, interferes with the pharmacokinetics of the antibiotic.

  11. Phosphoproteome Analysis Reveals the Molecular Mechanisms Underlying Deoxynivalenol-Induced Intestinal Toxicity in IPEC-J2 Cells

    Science.gov (United States)

    Zhang, Zhi-Qi; Wang, Song-Bo; Wang, Rui-Guo; Zhang, Wei; Wang, Pei-Long; Su, Xiao-Ou

    2016-01-01

    Deoxynivalenol (DON) is a widespread trichothecene mycotoxin that commonly contaminates cereal crops and has various toxic effects in animals and humans. DON primarily targets the gastrointestinal tract, the first barrier against ingested food contaminants. In this study, an isobaric tag for relative and absolute quantitation (iTRAQ)-based phosphoproteomic approach was employed to elucidate the molecular mechanisms underlying DON-mediated intestinal toxicity in porcine epithelial cells (IPEC-J2) exposed to 20 μM DON for 60 min. There were 4153 unique phosphopeptides, representing 389 phosphorylation sites, detected in 1821 phosphoproteins. We found that 289 phosphopeptides corresponding to 255 phosphoproteins were differentially phosphorylated in response to DON. Comprehensive Gene Ontology (GO) analysis combined with Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment revealed that, in addition to previously well-characterized mitogen-activated protein kinase (MAPK) signaling, DON exposure altered phosphatidylinositol 3-kinase/Akt (PI3K/Akt) and Janus kinase/signal transducer, and activator of transcription (JAK/STAT) pathways. These pathways are involved in a wide range of biological processes, including apoptosis, the intestinal barrier, intestinal inflammation, and the intestinal absorption of glucose. DON-induced changes are likely to contribute to the intestinal dysfunction. Overall, identification of relevant signaling pathways yielded new insights into the molecular mechanisms underlying DON-induced intestinal toxicity, and might help in the development of improved mechanism-based risk assessments in animals and humans. PMID:27669298

  12. Design of a Self Don/Doffing Rear Entry Planetary Suit to Interface with a Suit Port/Lock Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Under Phase 1 of subject SBIR, Air-Lock, Incorporated will design a self donning and doffing Rear Entry Hard Upper Torso (REHUT) that interfaces with a suit port....

  13. A Nutritional Approach for the Management of Deoxynivalenol (DON Toxicity in the Gastrointestinal Tract of Growing Chickens

    Directory of Open Access Journals (Sweden)

    Jürgen Zentek

    2008-12-01

    Full Text Available It has been shown that DON has negative effects on the active transport of some nutrients in the small intestine of chickens. The plausible interactions between food contaminants and natural components could be high. The present study investigated the effects of DON on the presence or absence of dietary inulin on the electrophysiological response of the gut to glucose. Ussing chamber studies were conducted with isolated jejunal epithelia at the age of 35 days. Electrophysiology of the epithelia was recorded and the changes of the short-circuit current (Isc were determined. The addition of Dglucose on the luminal side of the isolated mucosa increased (P 0.05. However, in the dietary inulin supplemented group in both jejunum and colon, the addition of glucose after preincubation of tissues with DON increased the Isc, suggesting that the dietary inulin supplementation of the broilers regulated and improved the glucose absorption in the presence of DON and kept it at normal levels.

  14. Bair Island Restoration and Management Plan : Don Edwards San Francisco Bay National Wildlife Refuge : Bair Island Ecological Reserve

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The U.S. Fish and Wildlife Service, Don Edwards San Francisco Bay National Wildlife Refuge, and the California Department of Fish and Game are proposing adoption of...

  15. University Mentality or Community College Paranoia: A Critique of Don Morgan's Reaction to David Riesman's Tentative Hypotheses.

    Science.gov (United States)

    Vaughan, George B.

    1979-01-01

    Responds to Don Morgan's reaction (JC 502 050) to David Riesman's article, "Community Colleges: Some Tentative Hypotheses" (JC 502 056). Summarizes Riesman's major arguments and challenges Morgan's interpretations of them. (AYC)

  16. Southern San Francisco Bay Colonial Nesting Bird Census 1995-1996: Don Edwards San Francisco Bay National Wildlife Refuge Lands

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes the 1995-1996 field season of the San Francisco Bay Bird Observatory (SFBBO) Colonial Waterbird Monitoring Study on the Don Edwards San...

  17. Upland habitat weed management plan for the Don Edwards San Francisco Bay National Wildlife Refuge Environmental Education Center

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The purpose of this plan is to outline the current status of invasive weeds at the Don Edwards San Francisco Bay NWR Environmental Education Center and to provide a...

  18. National Wildlife Refuge Visitor Survey 2012: Individual refuge results for Don Edwards San Francisco Bay National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes the National Wildlife Refuge Visitor Survey for Don Edwards San Francisco Bay National Wildlife Refuge and is part of the USGS Data Series...

  19. PENGGI'NAAN MEDIA GAMBAR DAII\\M PEMBELAJARAN MEMBACA KELAS I SD TRAIER DON BOSCO TARAKAN

    OpenAIRE

    Oberta Olvi Wastuti

    2011-01-01

    OBERTA OLVI WASTUTI 2010. PenggunaanMedia Gambar Dalam pembelajaran Membsca Kelot 1 SD Frater Don Bosco Toraltan. Kelurahan Pamusian, Keeernatan Tarakan tengan, Jl Patimura No 1 RT VIII, Pembimbing I H. Sofa, M.Pd, Pembimbing II Drs, Petrus Tena Bean. Tuj"an ,"n"r'* untuk mengetahui perbedaan hasil belajar membaca denean Menggunakan Media Gambar dan Tidak Menggunakan Media Gambar Siswa Kelas I SD Frater Don Bosco Tarakan. Pengambilan sampel, penulis mengambil semua populasi sebanyak ...

  20. MENINGKATKAN HASIL BELAJAR PKN MELALUI MEDIA BERBASIS TIK PADA SISWA KELAS V SD FRATER DON BOSCO TARAKAN

    OpenAIRE

    Ursula Tuto Kemaun

    2011-01-01

    "Tuto Kemaun Ursula 2011, PKN Improve Learning Outcomes hrough ICT-Based Media in class V student of Don Bosco Brother SD Tarakan Year 2010/2011 lessons. Classroom Action Research goal is to improve student learning outcomes in particular grade 5B SD Frater Don Bosco Tarakan through the medium of ICT-based learning civic education so that learning becomes more enjoyable, challenging, and lead to creativity. Design research is done through two cycles, starting from planning, implementation mea...

  1. 女性并不比男性唠叨%WOMEN DON'T REALLY TALK MORE

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    @@ Women don't talk more than men. Really, they don't. Popular wisdom would have it that women are much chattier than men, speaking 20,000 words a day, vs. the average men's 7,000. But a study being published shows that it is stereotype(陈规). Both men and women use about 16,000 words a day, says the new research, in Science magazine.

  2. Real men don't beat their wives!

    OpenAIRE

    Kaur, Dr Gurmeen

    2010-01-01

    Despite the numerous Domestic violence laws, women protection acts and women cells in India at present; physical abuse and domestic violence is still rampant and grossly underreported. This article describes my experience in dealing with victims of domestic abuse as a medical student.

  3. ["Is it an animal inside? "Melanie Klein's unpublished Don Juan Paper (1939)].

    Science.gov (United States)

    Frank, Claudia

    2008-01-01

    Klein had been asked to contribute an article to the birthday number of the International Journal for Jones. The author outlines how she hurriedly wrote a text about Don Juan which, however, was rejected by the editor. Essential parts of it are presented in German translation. The manuscript is discussed in the context of Klein's published work as well as of the relevant contemporary literature. In Klein's view, Don Juan's genitality is determined by oral impulses and fears. By his manic acting out he attempts to ward off a depressive break-down. The paper ends with some reflections about why Klein--ontrary to her intention--failed to revise her manuscript for later publication. PMID:19230330

  4. Migration of Near Earth Asteroid to Jovian-Crosser Asteroid:Case Study 3552 Don Quixote

    CERN Document Server

    Siregar, Suryadi

    2010-01-01

    It is generally recognized that main-belt asteroids (MBAs) and nuclei of extinct comets are the two main sources for the Near-Earth-Asteroids (NEAs). Theoretical studies of NEAs dynamics and numerical modelling of their orbital motions showed that the resonance mechanism for supplying NEAs is quite sufficient to sustain this population. Asteroid 1983 SA, also known as 3552 Don Quixote, is one of Near Earth Asteroids (NEAs) and the most probable candidates for NEAs of the cometary origin. In this work, an investigation on the evolution of the orbit is done by using the SWIFT subroutine package, where the gravitational perturbations of eight planets: Mercury, Venus, Earth, Mars, Jupiter, Saturn, Uranus, and Neptune are considered. Migration of asteroid 3552 Don Quixote from Near Earth Asteroids (NEAs) to Jovian-crosser asteroid is found.

  5. El palacio de don Antonio de Mendoza en Guadalajara. Aspectos decorativos y formales

    Directory of Open Access Journals (Sweden)

    Rodríguez Rebollo, Ángel

    2002-09-01

    Full Text Available The Palace of Don Antonio de Mendoza in Guadalajara was constructed at the beginning of the sixteenth century. In spite of the absence of documentation, there are clear stylistic similarities between this palace and the Calahorra Palace (Granada and the tomb of Cardinal Mendoza. The Codex Escurialensis was used for the decoration of the first floor column capitals as well as the façade decoration.

    El Palacio de Don Antonio de Mendoza en Guadalajara fue construido a principios del XVI. Pese a no estar documentado, las similitudes estilísticas con el Palacio de la Calahorra (Granada y el sepulcro del Cardenal Mendoza son claros. El Codex Excurialensis fue usado en la decoración de los capiteles del primer piso y en la decoración de la fachada principal.

  6. Don Quijote, doña Rodríguez y los duques

    Directory of Open Access Journals (Sweden)

    Stanislav Zimic

    1997-12-01

    Full Text Available Al llegar Don Quijote al palacio de los duques y "viéndose tratar del mesmo modo que él había leído se trataban Ios tales caballeros en Ios pasados siglos ... , aquél fue el primer día que de todo en todo conoció y creyó ser caballero andante verdadero y no fantastíco" (1378. Comprensiblemente, esta afirmacion ha causado gran perplejidad entre los lectores de todas las épocas: "Luego, antes ¿no lo había creído?" . La cuestión se complica mucho más, poco después de estos eufóricos momentos, al confesar Don Quijote a la duquesa: "Dios sabe si hay Dulcinea o no en el mundo, o si es fantástica ono es fantástica ...

  7. ["Is it an animal inside? "Melanie Klein's unpublished Don Juan Paper (1939)].

    Science.gov (United States)

    Frank, Claudia

    2008-01-01

    Klein had been asked to contribute an article to the birthday number of the International Journal for Jones. The author outlines how she hurriedly wrote a text about Don Juan which, however, was rejected by the editor. Essential parts of it are presented in German translation. The manuscript is discussed in the context of Klein's published work as well as of the relevant contemporary literature. In Klein's view, Don Juan's genitality is determined by oral impulses and fears. By his manic acting out he attempts to ward off a depressive break-down. The paper ends with some reflections about why Klein--ontrary to her intention--failed to revise her manuscript for later publication.

  8. On Test Data Compression Using Selective Don't-Care Identification

    Institute of Scientific and Technical Information of China (English)

    Terumine Hayashi; Haruna Yoshioka; Tsuyoshi Shinogi; Hidehiko Kita; Haruhiko Takase

    2005-01-01

    This paper proposes an effective method for reducing test data volume under multiple scan chain designs. The proposed method is based on reduction of distinct scan vectors using selective don't-care identification. Selective don't-care identification is repeatedly executed under condition that each bit of frequent scan vectors is fixed to binary values (0 or 1). Besides, a code extension technique is adopted for improving compression efficiency with keeping decompressor circuits simple in the manner that the code length for infrequent scan vectors is designed as double of that for frequent ones. The effectiveness of the proposed method is shown through experiments for ISCAS'89 and ITC'99 benchmark circuits.

  9. Detsibill : Meie Mees kui ANTI. White Label : Don Erikson. Kuula / DJ Pickney Tiger

    Index Scriptorium Estoniae

    DJ Pickney Tiger, pseud., 1970-

    2006-01-01

    Heliplaatidest: Don Erikson "Israel Mix", Congotronics 2 "Buzz'n'rumble from the urb'n'jungle", Critikal "Chapter One ehk Teine maitse", Nirvana "Silver, The best of the Box", Singer Vinger "Eesti Kullafond", Bullet Train To Vegas "We Put Scissors Where Our Mouths Are", Ludachris "Disturbing tha peace", "Soul Satisfaction 5", The Kurt Webber's Pink Band "Café Regatt", Patrizio Buanne "The Italian", Eminem "Curtain Call"

  10. Reproduction et Diversité Génétique chez Parkia biglobosa (Jacq.) G. Don

    OpenAIRE

    Sina, S.

    2006-01-01

    Parkiabiglobosa(Jacq.)G.Don, African locust bean, is one of the 34 known species of the genusParkiawhose centre of origin isSouth America. It is anagroforestrytree species spread throughout the Africansudaniansavannas, fromSenegalin the West toUgandain the East. P.biglobosa is also an important socio-economic species in high demand by local communities for its multiple amenities and uses (food, traditional medicine and pharmacy, preservation and improvement of soil fertility, rites and custom...

  11. STS-38 Mission Specialist (MS) Robert C. Springer dons EMU in JSC's WETF

    Science.gov (United States)

    1990-01-01

    STS-38 Mission Specialist (MS) Robert C. Springer dons extravehicular mobility unit (EMU) upper torso with technicians' assistance in JSC's Weightless Environment Training Facility (WETF) Bldg 29. Positioned on the WETF platform at pool side, Springer is preparing for an underwater extravehicular activity (EVA) simulation. During the training session, Springer will rehearse contingency EVA procedures for the STS-38 mission aboard Atlantis, Orbiter Vehicle (OV) 104.

  12. An Inventory of Tortricidae (Lepidoptera) from the Rostov-on-Don province of Russia.

    Science.gov (United States)

    Poltavsky, Alexander N

    2015-11-27

    Records of leafroller moths (Tortricidae) from the Rostov-on-Don province of southern Russia are presented based on 10 years of field work (2005-2014). The list includes 91 species from 43 collecting sites; 80 species are recorded from the region for the first time. Compared to other well-surveyed, adjacent areas, the tortricid fauna is considerably more depauperate, and this may be explained in part by the predominance of steppe vegetation.

  13. Heavy Metals and Biogenic Elements in Aquatic Systems of the Don River Delta

    Science.gov (United States)

    Tkachenko, Anna; Tkachenko, Oleg

    2014-05-01

    River deltas are located in the lower parts of the cascade landscape-geochemical systems of the river basins, so their geochemical conditions often characterize the anthropogenic impact on whole river system. The Don River runs through the one of the most agriculturally developed and densely populated area of Russia, and flows into the Azov Sea - the smallest and shallowest sea in the world. These factors determine the geochemical features of aquatic systems of the Don River mouth area and the specificity of the "river-sea" geochemical barrier zone. The paper presents results of the field studies of the geochemical structure of the Don River mouth area, which were conducted in frames of the RFBR project in 2012-2013. Major types of the deltaic water streams and bodies were studied in different hydrological seasons: spring floods, summer, autumn and winter low water periods. About 50 samples of water, suspended matter and 60 samples of bottom sediments have been collected and analyzed for heavy metals (Fe, Mn, Zn, Cu, Ni, Co, Pb, Cr, Cd etc.) and biogenic elements (nitrate, nitrite, ammonium, phosphates, silica, total nitrogen and phosphorus, dissolved oxygen and chlorophyll) content. To assess the toxicity degree and nutrient potential of water, bioassay test conducted by growing daphnia in water samples were held. The study shows that the Don River delta water is characterized by the relatively low values of dissolved heavy metal content. Significantly higher values of heavy metals were determined in the vicinity of settlements only. Metal accumulation in bottom sediments can be associated mainly with the rate of water flow. Higher values were found in sediments of small channels with weak flow velocity and prevailing processes of the suspended matter deposition. The data on the seasonal dynamics of nutrients and spatial variability of their forms have been obtained. The maximum concentration of nitrogen, phosphorus, silicon, and other biogenic elements are

  14. Cosmogenic 7Be in ground level air in Rostov-on-Don (Russia) (2001-2011)

    CERN Document Server

    Buraeva, E A; Nephedov, V C; Shramenko, B I; Stasov, V V; Zorina, L V

    2013-01-01

    The deposition flux of cosmogenic 7Be in the industrial city Rostov-on-Don (Russia) from 2001 to 2011 has been measured. The variations of annual 7Be deposition flux appear to be mainly correlated with the number of the meteorological parameters and solar activity. For the first time correlations of the volume activity of cosmogenic 7Be with such meteorological parameters as temperature, precipitation, wind speed, atmospheric pressure, relative humidity are identified.

  15. Application of gamma radiation to the nodes detection in Pinus Radiata (D.Don) wood pieces

    International Nuclear Information System (INIS)

    Attenuation of 59.5 KeV photons provided by an Am-241 source, has been used to detect knots in lumber pieces from Pinus Radiata (D.Don). It is shown that the linear attenuation coefficient is a sensitive parameter to detect singularities in the structure of this material. The scanning of the piece provides profiles which define the position and extension of the singularity. (author)

  16. The perception of plants in the complete works of Cervantes, particularly “Don Quijote”

    OpenAIRE

    Morales Valverde, Ramón; Pardo de Santayana, Manuel; Tardío, Javier

    2006-01-01

    We searched the complete works of Cervantes, as historical ethnobotanical resources, for all references to plants, plant communities and products. Our analysis of the perception and uses of plants in Spain 400 years ago covers wild trees and shrubs; cultivated plants in fields and gardens, mainly edible species; medicinal plants; plants with technological applications; and ornamental and symbolic plants. 167 species were registered in the 38 works, 110 cited in “Don Quijote”. The ...

  17. Enterovirus A71 Meningoencephalitis Outbreak, Rostov-on-Don, Russia, 2013

    OpenAIRE

    Akhmadishina, Ludmila V.; Govorukhina, Marina V.; Kovalev, Evgeniy V.; Nenadskaya, Svetlana A.; Ivanova, Olga E.; Lukashev, Alexander N.

    2015-01-01

    Seventy-eight cases of enterovirus infection, including 25 neuroinfections, occurred in Rostov-on-Don, Russia, during May–June 2013. The outbreak was caused by an enterovirus A type 71 (EV-A71) subgenotype C4 lineage that spread to neighboring countries from China ≈3 years earlier. Enterovirus associated neuroinfection may emerge in areas with a preceding background circulation of EV-A71 with apparently asymptomatic infection.

  18. Enterovirus A71 Meningoencephalitis Outbreak, Rostov-on-Don, Russia, 2013

    Science.gov (United States)

    Akhmadishina, Ludmila V.; Govorukhina, Marina V.; Kovalev, Evgeniy V.; Nenadskaya, Svetlana A.; Ivanova, Olga E.

    2015-01-01

    Seventy-eight cases of enterovirus infection, including 25 neuroinfections, occurred in Rostov-on-Don, Russia, during May–June 2013. The outbreak was caused by an enterovirus A type 71 (EV-A71) subgenotype C4 lineage that spread to neighboring countries from China ≈3 years earlier. Enterovirus associated neuroinfection may emerge in areas with a preceding background circulation of EV-A71 with apparently asymptomatic infection. PMID:26196217

  19. Reflections on Don Juan and on the utility of the unhappy love affair.

    Science.gov (United States)

    Bergel, Ernest

    2011-12-01

    Based on an unusual clinical experience of a teenage boy in child psychotherapy, two conclusions are proposed: (1) that the extremely unhappy, early love affairs that occur in most men's lives serve a valuable function in helping them separate from their mothers sufficiently to be able to realistically relate to appropriate marriage partners, and (2) that some Don Juans start new relationships in order to break them off, rather than the reverse.

  20. Review: Don't Go Up Kettle Creek: Verbal Legacy of the Upper Cumberland

    OpenAIRE

    1984-01-01

    In Don't Go Up Kettle Creek, Montell reconstructs the history of the Upper Cumberland River region "as it is perceived from the vernacular point of view, relying on personal reminiscences, oral traditions, balladry and song, and printed materials (which were themselves derived from oral history data) as primary sources of information" (p. 1). Although these oral sources provide the substance of the book, Montell corroborates the oral information wherever possible using more standard historica...

  1. Isolation and Characterization of Antineoplastic Alkaloids from Catharanthus Roseus L. Don. Cultivated in Egypt

    OpenAIRE

    Shams, Khaled A; Nazif, Naglaa M; Abdel Azim, Nahla S; Abdel Shafeek, Khaled A; El-Missiry, Mostafa M.; Ismail, Shams I; Seif El Nasr, Medhat M

    2009-01-01

    Vinblastine and vincristine (the antileukemic agents) were isolated, in a pure form, from Catharanthus roseus L. Don., cultivated in Egypt, by several chromatographic techniques. Five modified methods for the preparation of total alkaloids were carried out. All the isolated mixtures were evaluated by HPLC and HPTLC analyses. The antineoplastic alkaloids; vinblastine and vincristine, were isolated by the use of vacuum liquid chromatographic column on silica gel : aluminium oxide (1:1) mixed be...

  2. Effect of Salinity Stress on Seed Germination Catharanthus roseus Don. Cvs. Rosea and Alba

    OpenAIRE

    Zahra Rezaee; Mehrangiz Chehrazi; Norollah Moalemi

    2012-01-01

    This experiment was carried out aiming to determine the Catharanthus roseus Don resistance against salinity, due to the increasing salinity of soil, and the importance of this plant as an ornamental flower, as well as the little information available on its tolerance against salinity during the germination period. In order to an experiment was conducted in randomized completely design factorial. Sodium chloride was used for induce salinity stress. These factors include cultivar and salinity l...

  3. A new combination in Orophea (Annonaceae for Uvaria nitida Roxb. ex G. Don

    Directory of Open Access Journals (Sweden)

    Ian M. Turner

    2014-12-01

    Full Text Available The identity of Uvaria nitida Roxb. ex G.Don. (Annonaceae has not been considered for 180 years. The plant is only known from material grown in the Calcutta Botanic Garden in India following introduction from, reportedly, the Moluccas. Examination of a specimen from the Brussels Herbarium, designated here as lectotype, indicates that the species is a member of Orophea subgenus Sphaerocarpon, similar to Orophea gabra Merr. A new combination in Orophea is made. 

  4. En un lugar de Twitter: las andanzas del caballero don Quijote por la red del pajarito

    Directory of Open Access Journals (Sweden)

    Concepción Torres Begines

    2015-05-01

    Full Text Available Since the beginning of Twitter in 2006, we have attended to the born of a new way of writing according to the parameters imposed by the social network. Many studies have stated the existence of a new literary form known as twitterature in its most popular entry. In this article, we have tried to compile the most significant projects related to the role played by Don Quixote, the main work in the literature written in Spanish, in Twitter.

  5. Reproduction et Diversité Génétique chez Parkia biglobosa (Jacq.) G. Don

    NARCIS (Netherlands)

    Sina, S.

    2006-01-01

    Parkiabiglobosa(Jacq.)G.Don, African

  6. Migration of Near Earth Asteroid to Jovian-Crosser Asteroid:Case Study 3552 Don Quixote

    OpenAIRE

    Siregar, Suryadi

    2010-01-01

    It is generally recognized that main-belt asteroids (MBAs) and nuclei of extinct comets are the two main sources for the Near-Earth-Asteroids (NEAs). Theoretical studies of NEAs dynamics and numerical modelling of their orbital motions showed that the resonance mechanism for supplying NEAs is quite sufficient to sustain this population. Asteroid 1983 SA, also known as 3552 Don Quixote, is one of Near Earth Asteroids (NEAs) and the most probable candidates for NEAs of the cometary origin. In t...

  7. RNA干扰下调叉头转录因子M1基因对人肿瘤细胞生长、克隆和侵袭的影响%Effects of forkhead box protein M1 down-regulation by RNA interfence on growth,and don and invasion of human ovarian cancer cell

    Institute of Scientific and Technical Information of China (English)

    范钰; 徐娟; 周永静; 陈琳; 赵松兰

    2014-01-01

    Objective To study the effects of RNA interference forkhead box protein M1 (FoxM1) on growth,and clon formation,and invasion of huaman ovarian cancer cell.Methods Using checks The FoxM1 gene mRNA levels were exmined by real-time quantitative polymerase chain reaction (Real-time PCR) method of eight human ovarian cancer cell lines.FoxM1 small interfering RNA (siRNA)were built and synthesized by using chemical methods.The cells were classified into three groups:blank control group (Con-A),the empty plasmid group (Con-B) and FoxM1 siRNA group (siRNA).Among them,the siRNA group was transfected with FoxM1 siRNA.was used to detect cancer cells FoxM1 mRNA and protein levels in each group were detected by Real-time PCR and Western blotting,respectively.The cell viability of each group was measured by cell counting kit-8 (CCK-8) assay.The clones were evaluated by colony formation assay.The invasion ability was evaluated by Transwell method.Results The mRNA levels of FoxM1 gene is highest in the SKOV-3 and HO-8910PM cell lines.After SKOV-3 cell line was transfected by FoxM1 siRNA,the results of the CCK-8 assay showed that the A Values of Con-A,Con-B and FoxM1 group in 72 h were 2.455 ± 0.033,2.442 ± 0.025 and 1.312 ± 0.028,respectively (P < 0.05) ; the results of colony formation assay showed that clones of Con-A,Con-B and FoxM1 group were 100 ±5,93 ±8 and 51 ±3,respectively (P<0.05) ; the results of the Transwell showed that the membrane cell number of Con-A,Con-B and FoxM1 group were 93 ± 4,86 ± 3 and 36 ± 1,respectively (P <0.05).After HO-8910PM cell line was transfected by FoxM1 siRNA,the results of the CCK-8 assay showed that the A Values of Con-A,Con-B and FoxM1 group in 72 h were 2.691 ±0.039,2.560 ±0.033 and 1.455 ± 0.027,respectively (P < 0.05) ; the results of colony formation assay showed that clones of Con-A,Con-B and FoxM1 group were 146 ± 7,145 ± 8 and 85 ± 2,respectively (P < 0.05) ; the results of the Transwell showed that the

  8. Relative Distributions of Dreissena bugensis and Dreissena polymorpha in the Lower Don River System, Russia

    Science.gov (United States)

    Zhulidov, A. V.; Pavlov, D. F.; Nalepa, T. F.; Scherbina, G. H.; Zhulidov, D. A.; Gurtovaya, T. Yu.

    2004-07-01

    A survey was conducted in the lower Don River system in Russia to confirm the presence of Dreissena bugensis, and to compare its distribution relative to that of Dreissena polymorpha. In 1999 and 2001-2002, dreissenid mussels were collected at 15 sites in the main river, in connecting reservoirs, and in a major tributary, the Manych River. Collections were made near stations where long-term monitoring data on total mineral (sum of principal ions) and calcium content were available. Both dreissenid species were found at all sites, with D. bugensis comprising 4-75% of all dreissenids at individual sites. D. bugensis was relatively more abundant than D. polymorpha in the Manych River where total mineral and calcium content was significantly higher than in the Don River, suggesting the two species may have different calcium requirements. Examination of archived samples indicated that D. bugensis was present in the Don River system as early as the 1980s, presenting the unresolved enigma of why D. bugensis has not displaced D. polymorpha as the dominant species as typically found over shorter time periods in other water bodies. (

  9. MANUSCRITOS TRADUCTORIOS DEL BRASIL IMPERIAL. “LA ARAUCANA” DE DON PEDRO II

    Directory of Open Access Journals (Sweden)

    Ana María Barrera Conrad Sackl

    2014-06-01

    Full Text Available Resumen: Don Pedro II, emperador del Brasil entre 1840 y 1889, se dedicó con ahínco y pasión a la tarea traductora de varios idiomas hacia el portugués. Del español menciona en sus diarios dos trabajos, una traducción de "Granada" (1853 de Zorrilla, cuyo manuscrito aún no se ha encontrado y "La Araucana" de Don Alonso De Ercilla y Zúñiga (1558, que analizamos. El prototexto del último fue descubierto por el equipo de NUPROC (http://www.item.ens.fr/ en el IGHB de Rio de Janeiro en 2011, está fechado en 1889 y lleva la firma del emperador. El análisis se realiza desde la perspectiva teórica de la Crítica Genética y de los Estudios Descriptivos de la Traducción. Palabras claves: traducción; español; portugués; manuscritos de Don Pedro II. Manuscritos traduzidos no Brasil Imperial: La Araucana de Dom Pedro II Resumo: Don Pedro II, imperador do Brasil entre 1840 e 1889, dedicou-se com afinco e paixão à tarefa tradutória de vários idiomas para o português. Menciona em seus diários duas traduções do espanhol, Granada (1853 de Zorrilla, cujo manuscrito ainda não foi localizado e La Araucana de Dom Alonso de Ercilla y Zuñiga (1558 que analisamos neste artigo. O prototexto do último formado por três páginas foi descoberto pela equipe do NUPROC no IGBH de Rio de Janeiro em 2011, está assinada pelo imperador no ano de 1889. Os fundamentos teóricos da análise são a Crítica Genética e os Estudos Descritivos da Tradução. Palavras-chave: Tradução. Espanhol. Português. Manuscritos de Dom Pedro II. Translated manuscripts in Imperial Brasil: Don Pedro II's La Araucana Abstract: Don Pedro II, Emperor of Brazil between 1840 and 1889, devoted himself with zeal and passion to the task of translation into Portuguese texts from different languages. For his translation into Spanish he mentions in his diaries two works, a translation of Granada (1853 by Zorrilla, whose manuscript has not yet been found and La Araucana by Don

  10. El don: entre las prácticas intersticiales y el solidarismo

    Directory of Open Access Journals (Sweden)

    ADRIÁN SCRIBANO

    2014-08-01

    Full Text Available El presente trabajo busca hacer evidente al solidarismo como la "pérdida del don", conforme expuesto por Mauss, en el contexto de la situación colonial, de los procesos de expansión capitalista en la actualidad. Como objeto de análisis y mediación hermenéutica, hemos seleccionado a la Responsabilidad Social Empresarial (RSE como una forma del solidarismo cuyas prácticas llevan a un conjunto de situaciones que pueden describirse como pornográficas. Para lograr el objetivo explicitado, hemos seguido la siguiente estrategia argumentativa: a se sintetizan algunas pocas coordenadas de carácter teóricos que permitan entender la centralidad del don y se explicita nuestra idea de solidarismo, b se realiza un triple análisis: de la conceptualización de la RSE y de las experiencias de RSE de algunas corporaciones internacionales en Argentina; y c se exponen nuestras ideas sobre prácticas intersticiales en tanto hiatos que quiebran a la economía política de la moral como una totalidad que demanda la "pérdida" de todo tipo de don.

  11. Effects of temperature and time on deoxynivalenol (DON and zearalenone (ZON content in corn

    Directory of Open Access Journals (Sweden)

    Jauković Marko

    2014-01-01

    Full Text Available Fumonisins are Fusarium mycotoxins that occur in corn and corn-based foods and they have been implicated in several animal and human diseases. Their effect on human health is unclear, however, fumonisins are considered to be risk factors for cancer. Baking, frying, and extrusion cooking of corn at high temperatures (190°C reduce fumonisin concentrations in foods, with the amount of reduction achieved depending on cooking time, temperature, recipe, and other factors. The aim of this work was to evaluate the effectiveness of temperature (200 and 220 °C and time (15 and 20 min on the detoxification of corn flour deliberately contaminated with DON and ZON. After processing at 200°C for 15 min, an average of 12% and after 20 min an average of 15% of DON was lost. At 200°C ZON content was reduced by 22% (after 15 min and by 27% (after 20 min. Higher temperature (220°C did not significantly affect further reduction of DON or ZON content. The process was only partially effective in both cases. [Projekat Ministarstva nauke Republike Srbije, br. TR-31023 i br. TR-31053

  12. Change and persistence in land surface phenologies of the Don and Dnieper river basins

    International Nuclear Information System (INIS)

    The formal collapse of the Soviet Union at the end of 1991 produced major socio-economic and institutional dislocations across the agricultural sector. The picture of broad scale patterns produced by these transformations continues to be discovered. We examine here the patterns of land surface phenology (LSP) within two key river basins-Don and Dnieper-using AVHRR (Advanced Very High Resolution Radiometer) data from 1982 to 2000 and MODIS (Moderate Resolution Imaging Spectroradiometer) data from 2001 to 2007. We report on the temporal persistence and change of LSPs as summarized by seasonal integration of NDVI (normalized difference vegetation index) time series using accumulated growing degree-days (GDDI NDVI). Three land cover super-classes-forest lands, agricultural lands, and shrub lands-constitute 96% of the land area within the basins. All three in both basins exhibit unidirectional increases in AVHRR GDDI NDVI between the Soviet and post-Soviet epochs. During the MODIS era (2001-2007), different socio-economic trajectories in Ukraine and Russia appear to have led to divergences in the LSPs of the agricultural lands in the two basins. Interannual variation in the shrub lands of the Don river basin has increased since 2000. This is due in part to the better signal-to-noise ratio of the MODIS sensor, but may also be due to a regional drought affecting the Don basin more than the Dnieper basin.

  13. Cell Theory, Specificity, and Reproduction, 1837–1870

    OpenAIRE

    Müller-Wille, Staffan

    2010-01-01

    The cell is not only the structural, physiological, and developmental, but also the reproductive unit of life. So far, however, this aspect of the cell has received little attention by historians and philosophers of biology. I will argue that cell theory had far-reaching consequences for how biologists conceptualized the reproductive relationships between germs and adult organisms. Cell theory, as formulated by Theodor Schwann in 1839, implied that this relationship was a specific and lawful ...

  14. Turner syndrome: don't forget the vulva

    OpenAIRE

    Haidopoulos, Dimitrios; Bakolas, George; Michala, Lina

    2016-01-01

    Summary Turner syndrome (TS) has been linked to a number of autoimmune conditions, including lichen sclerosus (LS), at an estimated prevalence of 17%. LS is a known precursor to vulvar cancer. We present a case of vulvar cancer in a 44-year-old woman, who had previously complained of pruritus in the area, a known symptom of LS. Histology confirmed a squamous cell carcinoma with underlying LS. Vulvar assessment for the presence of LS should be undertaken regularly as part of the routine assess...

  15. Single-cell analysis - Methods and protocols

    Directory of Open Access Journals (Sweden)

    Carlo Alberto Redi

    2013-06-01

    Full Text Available This is certainly a timely volume in the Methods in molecular biology series: we already entered the synthetic biology era and thus we need to be aware of the new methodological advances able to fulfill the new and necessary needs for biologists, biotechnologists and nano-biotechnologists. Notably, among these, the possibility to perform single cell analysis allow researchers to capture single cell responses....

  16. Corticosteroids for dengue - why don't they work?

    Directory of Open Access Journals (Sweden)

    Thi Hanh Tien Nguyen

    Full Text Available BACKGROUND: Dysregulated immune responses may contribute to the clinical complications that occur in some patients with dengue. FINDINGS: In Vietnamese pediatric dengue cases randomized to early prednisolone therapy, 81 gene-transcripts (0.2% of the 47,231 evaluated were differentially abundant in whole-blood between high-dose (2 mg/kg prednisolone and placebo-treated patients two days after commencing therapy. Prominent among the 81 transcripts were those associated with T and NK cell cytolytic functions. Additionally, prednisolone therapy was not associated with changes in plasma cytokine levels. CONCLUSION: The inability of prednisolone treatment to markedly attenuate the host immune response is instructive for planning future therapeutic strategies for dengue.

  17. Corticosteroids for Dengue – Why Don't They Work?

    Science.gov (United States)

    Nguyen, Thi Hanh Tien; Nguyen, Than Ha Quyen; Vu, Tuan Trung; Farrar, Jeremy; Hoang, Truong Long; Dong, Thi Hoai Tam; Ngoc Tran, Van; Phung, Khanh Lam; Wolbers, Marcel; Whitehead, Stephen S.; Hibberd, Martin L.

    2013-01-01

    Background Dysregulated immune responses may contribute to the clinical complications that occur in some patients with dengue. Findings In Vietnamese pediatric dengue cases randomized to early prednisolone therapy, 81 gene-transcripts (0.2% of the 47,231 evaluated) were differentially abundant in whole-blood between high-dose (2 mg/kg) prednisolone and placebo-treated patients two days after commencing therapy. Prominent among the 81 transcripts were those associated with T and NK cell cytolytic functions. Additionally, prednisolone therapy was not associated with changes in plasma cytokine levels. Conclusion The inability of prednisolone treatment to markedly attenuate the host immune response is instructive for planning future therapeutic strategies for dengue. PMID:24349598

  18. Mercedes y privilegios para consolidar un mayorazgo indiano: de don Pedro Moctezuma Tlacahuepantzin a don Pedro Tesifón Moctezuma, primer conde de Moctezuma (1569-1639

    Directory of Open Access Journals (Sweden)

    Francisco Luis Jiménez Abollado

    2015-06-01

    Full Text Available Las adjudicaciones de mercedes y privilegios se convirtieron para los descendientes de Moctezuma II en una forma de sobrevivir a lo largo del período virreinal. Para ello esgrimieron razonamientos fundamentados en su ascendencia, la transmisión pacífica a la Corona hispánica de su patrimonio territorial, así como una presumible pobreza o necesidad. Este análisis se centrará en los sucesores del único hijo varón de Moctezuma que sobrevivió a la conquista castellana: don Pedro Moctezuma Tlacahuepantzin. Las adjudicaciones de mercedes, ayudas de costas y encomiendas vacas fueron continuas entre el último tercio del siglo xvi hasta el fin del período virreinal.

  19. Turner syndrome: don't forget the vulva

    Science.gov (United States)

    Haidopoulos, Dimitrios; Bakolas, George

    2016-01-01

    Summary Turner syndrome (TS) has been linked to a number of autoimmune conditions, including lichen sclerosus (LS), at an estimated prevalence of 17%. LS is a known precursor to vulvar cancer. We present a case of vulvar cancer in a 44-year-old woman, who had previously complained of pruritus in the area, a known symptom of LS. Histology confirmed a squamous cell carcinoma with underlying LS. Vulvar assessment for the presence of LS should be undertaken regularly as part of the routine assessments proposed for adult TS women. If LS is identified, then the patient should be warned of the increased risk of vulvar cancer progression and should be monitored closely for signs of the condition. Learning points Patients with TS are at increased risk of developing LS.LS is a known precursor to vulvar cancer.TS women with LS may be at risk of developing vulvar cancer and should be offered annual vulvar screening and also be aware of signs and symptoms of early vulvar cancer. PMID:27252865

  20. Don Quijote de la Mancha: Reflexiones en torno a las relaciones cine-literatura Don Quijote de la Mancha: Reflections about the relationships between cinematography-literature

    Directory of Open Access Journals (Sweden)

    Felipe Ardila Rojas

    2008-06-01

    Full Text Available Este artículo presenta un análisis de algunos aspectos de la relación cine-literatura, a partir del Don Quijote de La Mancha literario y de algunas versiones cinematográficas y televisivas realizadas en español y en inglés. Inicialmente se presentan problemas teóricos en torno a categorías que suelen serles afines: relato, autor, narración, historia, etc., para involucrar alternativamente fenómenos que, desde el punto de vista de los autores, generan ambigüedades textuales o múltiples interpretaciones en las versiones que se analizan. De este análisis comparativo que parte de los relatos en cuestión se llega finalmente a conclusiones de carácter estético e ideológico sobre los mismos.This article presents an analysis of some aspects of the relationship between cinematography and literature. Starting from the Don Quijote de La Mancha and taking into account some film and television versions developed in Spanish and English. At the beginning, a theoretical problem based on similar types for concepts categorization: author, narration, story, relat, among others will be analyzed. In addition, a connection is made with some authors' concepts in order to avoid textual ambiguity or multiple interpretations in the versions that are analyzed. Through a comparative analysis supported in these stories, it is possible to reach some aesthetic and ideological conclusions about them.

  1. Effects of deoxynivalenol (DON) in the lactation diet on the feed intake and fertility of sows.

    Science.gov (United States)

    Gutzwiller, Andreas

    2010-08-01

    A diet contaminated with 2.8 mg deoxynivalenol (DON)/kg was fed at 6 kg per day to 32 mycotoxin-exposed pluriparous sows (M) during lactation. The 31 control sows (C) received 6 kg of an uncontaminated diet. Although more contaminated diet was refused (P = 0.05), DON exposure had no effect (P > 0.1) on body weight loss of the sows during lactation (M: 27.9 ± 12.3 kg; C: 29.7 ± 10.2 kg), the number of weaned piglets (M: 9.8 ± 1.4; C: 9.7 ± 1.6) and their daily weight gain (M: 266 ± 70 g; C: 272 ± 64 g). Several sows were culled after weaning for reasons unrelated to the experiment. Compared with the remaining 21 C sows, the remaining 26 M sows had an identical interval between weaning and the next farrowing (M: 120 ± 1 days; C: 120 ± 1 days) and a similar litter size (M: 14.5 ± 2.7; C: 14.9 ± 3.0; P > 0.10). The daily intake of 17 mg DON during lactation thus did not affect the reproductive performance of the sows.

  2. Survey of genome sequences in a wild sweet potato, Ipomoea trifida (H. B. K.) G. Don

    OpenAIRE

    Hirakawa, Hideki; Okada, Yoshihiro; Tabuchi, Hiroaki; Shirasawa, Kenta; Watanabe, Akiko; Tsuruoka, Hisano; Minami, Chiharu; Nakayama, Shinobu; Sasamoto, Shigemi; Kohara, Mitsuyo; Kishida, Yoshie; Fujishiro, Tsunakazu; Kato, Midori; Nanri, Keiko; Komaki, Akiko

    2015-01-01

    Ipomoea trifida (H. B. K.) G. Don. is the most likely diploid ancestor of the hexaploid sweet potato, I. batatas (L.) Lam. To assist in analysis of the sweet potato genome, de novo whole-genome sequencing was performed with two lines of I. trifida, namely the selfed line Mx23Hm and the highly heterozygous line 0431-1, using the Illumina HiSeq platform. We classified the sequences thus obtained as either ‘core candidates’ (common to the two lines) or ‘line specific’. The total lengths of the a...

  3. Is the Partner the Don Quixote of the 21st Century Corporate World?

    DEFF Research Database (Denmark)

    Bévort, Frans; Poulfelt, Flemming

    2015-01-01

    A large and growing proportion of the organizations in the contemporary knowledge economy are organized as professional partnerships as is the case of professional service firms (PSFs). As these firms have grown larger (e. g. Big4: Deloitte, PwC, E&Y, KPMG), the way of organizing is under pressure...... for change. This raises the question of whether the Partner ownership and management model is a relic of the past - and the partner a Don Quixote-figure of the 21st century corporate world? Based on an ethnographic study of a PSF in Denmark, and data from other sources, the paper analyzes the changing...

  4. El arzobispado de Don Gonzalo de Aguilar en Santiago de Compostela (1348-1351)

    OpenAIRE

    Sánchez Sánchez, Xosé M.

    2010-01-01

    In the middle of the 14th century the prelature of Santiago de Compostela is occupied by Don Gonzalo de Aguilar, archbishop transferred from Sigüenza and whose pontificate is extended between 1348 and 1351, when he is transferred to Toledo. He is a figure that remained until now in the complete darkness of the history of the Compostellan church, disappearing in fact, in many cases, of the historical considerations, that they avoided him or simply they did not have noticed of his figure, exten...

  5. Don DeLillo : une bibliographie sélective

    Directory of Open Access Journals (Sweden)

    Aaron Smith

    2006-03-01

    Full Text Available Oeuvres de Don DeLilloRomansAmericana. 1971. London : Penguin, 1990.End Zone. 1972. New York : Penguin, 1986.Great Jones Street. 1973. New York : Penguin, 1994.Ratner’s Star. 1976. London : Vintage, 1991.Players. 1977. London : Vintage, 1991.Running Dog. 1978. London : Picador, 1992.The Names. 1982. New York : Vintage, 1989.White Noise. 1984. London : Picador, 1985.Libra. 1988. London : Penguin, 1989.Mao II. 1991. London : Vintage, 1992.Underworld. 1997. New York : Scribner, 1997.The Body Art...

  6. Don DeLillo: Mao II / Underworld / Falling Man. Ed. Stacey Olster.

    Directory of Open Access Journals (Sweden)

    Angeliki Tseti

    2011-10-01

    Full Text Available The latest volume of the Continuum Studies in Contemporary North American Fiction is dedicated to three of the most influential recent novels written by Don DeLillo: Mao II, Underworld and Falling Man. These novels are set in pivotal moments of recent history, the end of the Cold War, the millennium and 9/11 and, so, offer fertile ground for the investigation of themes, such as terrorism, consumerism and globalism, that are dominant in the author’s entire oeuvre; what is more, the contributio...

  7. Stokes IQUV Magnetic Doppler Imaging of Ap stars I. ESPaDOnS and NARVAL Observations

    OpenAIRE

    Silvester, J.; Wade, G. A.; Kochukhov, O.; Bagnulo, S.; Folsom, C. P.; Hanes, D.

    2012-01-01

    In this paper we describe and evaluate new spectral line polarisation observations obtained with the goal of mapping the surfaces of magnetic Ap stars in great detail. One hundred complete or partial Stokes IQUV sequences, corresponding to 297 individual polarised spectra, have been obtained for 7 bright Ap stars using the ESPaDOnS and NARVAL spectropolarimeters. The targets span a range of mass from approximately 1.8 to 3.4 solar mass, a range of rotation period from 2.56 to 6.80 days, and a...

  8. A Critique on Katie's Language in Mockingbird Don't Sing

    Institute of Scientific and Technical Information of China (English)

    彭巧兰; 闵家顺

    2016-01-01

    This paper aims at critiquing Katie's language in the famous film, Mockingbird Don't Sing, which is a 2001 American independent film based on the true story of Genie, a modern-day feral child, discovered in California in 1970. The analyses of Katie's language are based on Katie's language by the stimulation of some people around her after she escaped from her home according to the theories of the interactionist position, Behaviorism, the affective filter hypothesis, Innatism, the stages of lan-guage development and the critical period hypothesis. Katie's abnormal language bitterly proves the importance of critical peri-od for language acquisition and language learning.

  9. Don Bosco, animator za zvanja. Povijesna razmišljanja za salezijanski pastoral zvanja

    OpenAIRE

    Canino Zanoletty, Miguel

    2011-01-01

    Neprijateljsko okruženje koje je često obilježavalo europsku panoramu u 19. st. imalo je teške posljedice na promicanje zvanja. U razdoblju u kojemu se uopće nije razvijao sustavni pastoral zvanja, don Bosco se osjetio posebno ponukanim da djelotvorno odgovori na takvo stanje. Iako nije bio prvi, njegova osoba i stil nizom su ga pojedinosti učinile nadaleko priznatim animatorom za zvanja. Uspio je oduševiti i privuči brojne osobe u odgojno poslanje i evangelizaciju mladeži. U svom djelovan...

  10. El sistema preventivo de Don Bosco, respuesta a la escuela multicultural

    OpenAIRE

    Arenal Jorquera, María José

    2009-01-01

    El objetivo de este estudio es estudiar si el Sistema Preventivo de Don Bosco, que se lleva en todos los centros salesianos de los cinco continentes, da respuesta a la escuela multicultural actual. Y esto se produce como consecuencia de que las escuelas salesianas hacen realidad un medio escolar intercultural basado en el paradigma holístico, que se adapta al contexto de cada momento histórico en tiempo y espacio. El punto de partida del mencionado sistema educativo surge en la realidad s...

  11. Astronaut Dunbar adjusts CCA before donning EMU helmet in JSC's WETF Bldg 29

    Science.gov (United States)

    1990-01-01

    Astronaut Bonnie J. Dunbar, wearing extravehicular mobility unit (EMU), adjusts the communications carrier assembly (CCA) ('Snoopy' cap) microphones before donning EMU helmet. Dunbar is preparing for an underwater simulation in JSC's Weightless Environment Training Facility (WETF) Bldg 29 pool. When fully suited, Dunbar will be lowered into the pool to rehearse planned and contingency extravehicular activities (EVAs). NOTE: Since this photograph was taken, Dunbar has been named as the payload commander (PLC) for STS-50 United States Microgravity Laboratory 1 (USML-1) mission aboard Columbia, Orbiter Vehicle (OV) 102.

  12. A EMERGÊNCIA DA ENTIDADE DON JUAN NA OBRA DE MILAN KUNDERA

    Directory of Open Access Journals (Sweden)

    Wilton Barroso*

    2010-12-01

    Full Text Available Este estudo tem por objetivo uma análise acerca do comportamento libertino1 dos don Juans2 kunderianos. De posse da compreensão do percurso dessa personagem na historiografia literária, objetiva-se uma comparação analítica dessa figura com as composições de Milan Kundera, tendo como centro de observação os seus universos objetivos e subjetivos de onde se acredita, emanam grandes temas vividos pela humanidade nos tempos atuais. PALAVRAS-CHAVE: Donjuanismo; Pós-modernidade; Subjetividade.

  13. The Knight-errant and their Itinerary. Journey and />Death of Don Quixote

    Directory of Open Access Journals (Sweden)

    Nieves Rodríguez Valle

    2016-01-01

    Full Text Available This article analyzes, from the perspective of the hero’s journey topic in the narrative, the wanderings of Don Quixote on the Spanish roads and in the spaces of the chivalric literature. Based on the four narrative sequences that stake out the itinerary in the journey narrative: departure, cruise, meeting, and return, the three departures and the three returns of the knight in the work are reviewed, specially the last return and the relationship that it keeps with his death.

  14. Glutamine analogs promote cytoophidium assembly in human and Drosophila cells

    Institute of Scientific and Technical Information of China (English)

    Kangni Chen; Jing Zhang; (O)mür Yilmaz Tastan; Zillah Anne Deussen; Mayte Yu-Yin Siswick; Ji-Long Liu

    2011-01-01

    CTP synthase is compartmentalized within a subcellular structure,termed the cytoophidium,in a range of organisms including bacteria,yeast,fruit fly and rat.Here we show that CTP synthase is also compartmentalized into cytoophidia in human cells.Surprisingly,the occurrence of cyloophidia in human cells increases upon treatment with a glutamine analog 6-diazo-5-oxo-L-norleucine (DON),an inhibitor of glutaminedependent enzymes including CTP synthase.Experiments in flies confirmned that DON globally promotes cytoophidium assembly.Clonal analysis via CTP synthase RNA interference in somatic cells indicates that CTP synthase expression level is critical for the formation of cytoophidia.Moreover,DON facilitates cytoophidium assembly even when CTP synthase level is low.A second glutamine analog azaserine also promotes cytoophidum formation.Our data demonstrate that glutamine analogs serve as useful tools in the study of cytoophidia.

  15. The fusarium mycotoxin deoxynivalenol can inhibit plant apoptosis-like programmed cell death.

    Directory of Open Access Journals (Sweden)

    Mark Diamond

    Full Text Available The Fusarium genus of fungi is responsible for commercially devastating crop diseases and the contamination of cereals with harmful mycotoxins. Fusarium mycotoxins aid infection, establishment, and spread of the fungus within the host plant. We investigated the effects of the Fusarium mycotoxin deoxynivalenol (DON on the viability of Arabidopsis cells. Although it is known to trigger apoptosis in animal cells, DON treatment at low concentrations surprisingly did not kill these cells. On the contrary, we found that DON inhibited apoptosis-like programmed cell death (PCD in Arabidopsis cells subjected to abiotic stress treatment in a manner independent of mitochondrial cytochrome c release. This suggested that Fusarium may utilise mycotoxins to suppress plant apoptosis-like PCD. To test this, we infected Arabidopsis cells with a wild type and a DON-minus mutant strain of F. graminearum and found that only the DON producing strain could inhibit death induced by heat treatment. These results indicate that mycotoxins may be capable of disarming plant apoptosis-like PCD and thereby suggest a novel way that some fungi can influence plant cell fate.

  16. Gender differences in national assessment of educational progress science items: What does i don't know really mean?

    Science.gov (United States)

    Linn, Marcia C.; de Benedictis, Tina; Delucchi, Kevin; Harris, Abigail; Stage, Elizabeth

    The National Assessment of Educational Progress Science Assessment has consistently revealed small gender differences on science content items but not on science inquiry items. This assessment differs from others in that respondents can choose I don't know rather than guessing. This paper examines explanations for the gender differences including (a) differential prior instruction, (b) differential response to uncertainty and use of the I don't know response, (c) differential response to figurally presented items, and (d) different attitudes towards science. Of these possible explanations, the first two received support. Females are more likely to use the I don't know response, especially for items with physical science content or masculine themes such as football. To ameliorate this situation we need more effective science instruction and more gender-neutral assessment items.

  17. Don Yllán and the Egyptian Sorcerer: Vernacular commonality and literary diversity in medieval Castile

    Directory of Open Access Journals (Sweden)

    Wacks, David A.

    2005-12-01

    Full Text Available In this article the author compares the exemplo of Don Yllán and the Deán de Santiago -no. 11 in Don Juan Manuel's Conde Lucanor (ca. 1335- with an earlier Hebrew analogue found in the Hebrew Mešal Haqadmonī (ca. 1285 of fellow Castilian author Isaac ibn Sahula. A thorough analysis of the rhetorical and narrative style of both versions reveals that the two tales shared a common source in Castilian oral tradition. The appearance of the tale in an earlier Hebrew text from Castile (the only other known version in any language calls into question the originality of Don Juan Manuel's most famous exemplo, suggesting a productive interplay between a common oral tradition in Castilian and coexisting literary traditions in Hebrew and Castilian.

    En este artículo, el autor compara el exemplo de Don Yllán y el Deán de Santiago -n.° 11 en el Conde Lucanor (ca. 1335 de Don Juan Manuel- con una versión anterior en la obra hebrea, Mešal Haqadmonī (ca. 1285 de otro autor castellano, Isaac ibn Sahula. Un análisis cuidadoso del estilo narrativo y retórico de ambas versiones revela que las dos comparten una fuente común en la tradición oral castellana. La aparición del cuento en un texto hebreo anterior de Castilla (la única versión conocida en cualquier otra lengua cuestiona la originalidad literaria del más famoso exemplo de Don Juan Manuel, y sugiere un intercambio productivo entre una tradición oral común castellana y tradiciones literarias hebreas y castellanas coexistentes.

  18. Growth performance, serum biochemical profile, jejunal morphology, and the expression of nutrients transporter genes in deoxynivalenol (DON)- challenged growing pigs

    OpenAIRE

    Li WU; Liao, Peng; He, Liuqin; Ren, Wenkai; Yin, Jie; Duan, Jielin; Li, Tiejun

    2015-01-01

    Background Fusarium infection with concurrent production of deoxynivalenol (DON) causes an increasing safety concern with feed worldwide. This study was conducted to determine the effects of varying levels of DON in diets on growth performance, serum biochemical profile, jejunal morphology, and the differential expression of nutrients transporter genes in growing pigs. Results A total of twenty-four 60-day-old healthy growing pigs (initial body weight = 16.3 ± 1.5 kg SE) were individually hou...

  19. The Influence of Processing by Impulse Pressure on the Productivity of the Don Barley (Hordeum vulgare L.

    Directory of Open Access Journals (Sweden)

    Pavlova Violetta Aleksandrovna

    2014-04-01

    Full Text Available Plant productivity is the important indicator, which determines the amount of yield. The productivity of plants depends on the number of bruchids per plant and on the weight of 1000 bruchids. The article studies the influence of impulse pressure of various magnitudes on plant productivity of Don barley (Hordeum vulgare L.. It was found that the pressure of 17 MPa was the most effective for increasing the productivity. Impulse pressure of other magnitudes also had influence on the productivity of Don barley.

  20. Patterns of dissolved organic carbon (DOC and nitrogen (DON fluxes in deciduous and coniferous forests under historic high nitrogen deposition

    Directory of Open Access Journals (Sweden)

    S. Sleutel

    2009-07-01

    Full Text Available Numerous recent studies have indicated that dissolved organic carbon (DOC and nitrogen (DON play an important role in C and N cycling in natural ecosystems, and have shown that N deposition alters the concentrations and fluxes of dissolved organic substances and may increase leaching losses from forests. Our study was set up to accurately quantify concentrations and flux patterns of DOC, DON and dissolved inorganic nitrogen (DIN in deciduous and coniferous forest in Flanders under historical high nitrogen deposition. We measured DOC, DON and DIN concentrations at two weekly intervals in a silver birch (SB stand, a corsican pine (CP stand and a pine stand with higher N deposition (CPN, and used the SWAP model (calibrated with PEST for generating accurate water and matter fluxes. The input with precipitation was an important source of DON, but not for DOC. Release of DOC from the forest floor was minimally affected by forest type, but higher N deposition (CPN stand caused an 82% increase of DOC release from the forest floor. Adsorption to mineral soil material rich in iron and/or aluminum oxyhydroxides was suggested to be the most important process removing DOC from the soil solution, responsible for substantial retention (67–84% of DOC entering the mineral soil profile with forest floor leachate. Generally, DON was less reactive (i.e. less removal from the soil solution than DOC, resulting in decreasing DOC/DON ratios with soil depth. We found increased DOC retention in the mineral soil as a result of higher N deposition (84 kg N ha−1 yr−1 additional DOC retention in CPN compared to CP. Overall DON leaching losses were 2.2, 3.3 and 5.0 kg N ha−1 yr−1 for SB, CP and CPN, respectively, contributing between 9–28% to total dissolved N (TDN leaching. DON loss from SB and CP was not much higher than from unpolluted forests, and its relative contribution to TDN leaching was mainly determined by

  1. Don't play the butter notes’: jazz in medical education

    Science.gov (United States)

    Bradner, Melissa; Harper, Darryl V.; Ryan, Mark H.; Vanderbilt, Allison A.

    2016-01-01

    Jazz has influenced world music and culture globally – attesting to its universal truths of surviving, enduring, and triumphing over tragedy. This begs the question, what can we glean in medical education from this philosophy of jazz mentoring? Despite our training to understand disease and illness in branching logic diagrams, the human experience of illness is still best understood when told as a story. Stories like music have tempos, pauses, and silences. Often they are not linear but wrap around the past, future, and back to the present, frustrating the novice and the experienced clinician in documenting the history of present illness. The first mentoring lesson Hancock discusses is from a time he felt stuck with his playing – his sound was routine. Miles Davis told him in a low husky murmur, ‘Don't play the butter notes’. In medical education, ‘don't play the butter notes’ suggests not undervaluing the metacognition and reflective aspects of medical training that need to be fostered during the early years of clinical teaching years. PMID:27095009

  2. 'Don't play the butter notes': jazz in medical education.

    Science.gov (United States)

    Bradner, Melissa; Harper, Darryl V; Ryan, Mark H; Vanderbilt, Allison A

    2016-01-01

    Jazz has influenced world music and culture globally - attesting to its universal truths of surviving, enduring, and triumphing over tragedy. This begs the question, what can we glean in medical education from this philosophy of jazz mentoring? Despite our training to understand disease and illness in branching logic diagrams, the human experience of illness is still best understood when told as a story. Stories like music have tempos, pauses, and silences. Often they are not linear but wrap around the past, future, and back to the present, frustrating the novice and the experienced clinician in documenting the history of present illness. The first mentoring lesson Hancock discusses is from a time he felt stuck with his playing - his sound was routine. Miles Davis told him in a low husky murmur, 'Don't play the butter notes'. In medical education, 'don't play the butter notes' suggests not undervaluing the metacognition and reflective aspects of medical training that need to be fostered during the early years of clinical teaching years.

  3. Ecfrasis literaria y arquitectura novelesca: Los cuadernos de don Rigoberto de Mario Vargas Llosa

    Directory of Open Access Journals (Sweden)

    Pedro Antonio Agudelo

    2013-07-01

    Full Text Available Este artículo propone una relectura de la novela Los cuadernos de don Rigoberto de Mario Vargas Llosa, concentrando la atención en la figura retórica de la ecfrasis. Examina la arquitectura novelesca y la representación verbal y visual como pilares de lo que constituiría una genuina novela iconoverbal. El estudio está soportado en las estrategias de análisis provenientes de la semiótica literaria, la narratología y la retórica, que permiten una comprensión amplia del objeto de estudio. This paper proposes a different interpretation of the novel Los cuadernos de don Rigoberto by Mario Vargas Llosa, focusing on the rhetorical figure of ekphrasis. It examines the novelistic architecture and verbal and visual representation as pillars of what would constitute a genuine iconoverbal novel. The study is supported by the analysis strategies from literary semiotics, narratology and rhetoric, which allow a comprehensive understanding of subject matter.

  4. Finite element analysis of donning procedure of a prosthetic transfemoral socket.

    Science.gov (United States)

    Lacroix, Damien; Patiño, Juan Fernando Ramírez

    2011-12-01

    Lower limb amputation is a severe psychological and physical event in a patient. A prosthetic solution can be provided but should respond to a patient-specific need to accommodate for the geometrical and biomechanical specificities. A new approach to calculate the stress-strain state at the interaction between the socket and the stump of five transfemoral amputees is presented. In this study the socket donning procedure is modeled using an explicit finite element method based on the patient-specific geometry obtained from CT and laser scan data. Over stumps the mean maximum pressure is 4 kPa (SD 1.7) and the mean maximum shear stresses are 1.4 kPa (SD 0.6) and 0.6 kPa (SD 0.3) in longitudinal and circumferential directions, respectively. Locations of the maximum values are according to pressure zones at the sockets. The stress-strain states obtained in this study can be considered more reliable than others, since there are normal and tangential stresses associated to the socket donning procedure.

  5. Open source pipeline for ESPaDOnS reduction and analysis

    Science.gov (United States)

    Martioli, Eder; Teeple, Doug; Manset, Nadine; Devost, Daniel; Withington, Kanoa; Venne, Andre; Tannock, Megan

    2012-09-01

    OPERA is a Canada-France-Hawaii Telescope (CFHT) open source collaborative software project currently under development for an ESPaDOnS echelle spectro-polarimetric image reduction pipeline. OPERA is designed to be fully automated, performing calibrations and reduction, producing one-dimensional intensity and polarimetric spectra. The calibrations are performed on two-dimensional images. Spectra are extracted using an optimal extraction algorithm. While primarily designed for CFHT ESPaDOnS data, the pipeline is being written to be extensible to other echelle spectrographs. A primary design goal is to make use of fast, modern object-oriented technologies. Processing is controlled by a harness, which manages a set of processing modules, that make use of a collection of native OPERA software libraries and standard external software libraries. The harness and modules are completely parametrized by site configuration and instrument parameters. The software is open- ended, permitting users of OPERA to extend the pipeline capabilities. All these features have been designed to provide a portable infrastructure that facilitates collaborative development, code re-usability and extensibility. OPERA is free software with support for both GNU/Linux and MacOSX platforms. The pipeline is hosted on SourceForge under the name "opera-pipeline".

  6. Influence of microstructured carbon materials on Sequoia sempervirens (D. Don. Endl. in vitro culture

    Directory of Open Access Journals (Sweden)

    Raluca Stoiculescu

    2009-12-01

    Full Text Available The present experimental research has been mainly focused on Sequoia sempervirens (D. Don. Endl. as woody plant model system, for testing the influence of some microstructured carbon materials (CCM comparing with the activated charcoal action, on the in vitro growth. The work hypothesis was that CCM can induce effects on the plant culture, similar to those induced by activated charcoal, due to their high absorption capacity. The influence of two types CCM and activated charcoal introduced in the medium culture was evaluated on the fresh weight and on the length of the in vitro regenerants, after 30 and 60 days of cultivation. Our data revealed that the CCM is not toxic and does not inhibit plant growth. We also remarked that S. sempervirens (D. Don. Endl. was more reactive to the activated charcoal into the nutritive medium than the CCM compounds. The use of these new microstructured compounds is an important step both in the study of materials science and in the field of plant biotechnologies.

  7. Influence of microstructured carbon materials on Sequoia sempervirens (D. Don. Endl. in vitro culture

    Directory of Open Access Journals (Sweden)

    Raluca Stoiculescu

    2009-11-01

    Full Text Available The present experimental research has been mainly focused on Sequoiasempervirens (D. Don. Endl. as woody plant model system, for testing the influence of some microstructured carbon materials (CCM comparing with the activated charcoal action, on the in vitro growth. The work hypothesis was that CCM can induce effects on the plant culture, similar to those induced by activated charcoal, due totheir high absorption capacity. The influence of two types CCM and activated charcoal introduced in the medium culture was evaluated on the fresh weight and on the length of the in vitro regenerants, after 30 and 60 days of cultivation. Our data revealed that the CCM is not toxic and does not inhibit plant growth. We also remarked that S. sempervirens (D. Don. Endl. was more reactive to the activatedcharcoal into the nutritive medium than the CCM compounds. The use of these new microstructured compounds is an important step both in the study of materials science and in the field of plant biotechnologies.

  8. 'Don't play the butter notes': jazz in medical education.

    Science.gov (United States)

    Bradner, Melissa; Harper, Darryl V; Ryan, Mark H; Vanderbilt, Allison A

    2016-01-01

    Jazz has influenced world music and culture globally - attesting to its universal truths of surviving, enduring, and triumphing over tragedy. This begs the question, what can we glean in medical education from this philosophy of jazz mentoring? Despite our training to understand disease and illness in branching logic diagrams, the human experience of illness is still best understood when told as a story. Stories like music have tempos, pauses, and silences. Often they are not linear but wrap around the past, future, and back to the present, frustrating the novice and the experienced clinician in documenting the history of present illness. The first mentoring lesson Hancock discusses is from a time he felt stuck with his playing - his sound was routine. Miles Davis told him in a low husky murmur, 'Don't play the butter notes'. In medical education, 'don't play the butter notes' suggests not undervaluing the metacognition and reflective aspects of medical training that need to be fostered during the early years of clinical teaching years. PMID:27095009

  9. Leakage Current Optimization Techniques During Test Based on Don't Care Bits Assignment

    Institute of Scientific and Technical Information of China (English)

    Wei Wang; Yu Hu; Yin-He Han; Xiao-Wei Li; You-Sheng Zhang

    2007-01-01

    It is a well-known fact that test power consumption may exceed that during functional operation. Leakage power dissipation caused by leakage current in Complementary Metal-Oxide-Semiconductor (CMOS) circuits during test has become a significant part of the total power dissipation. Hence, it is important to reduce leakage power to prolong battery life in portable systems which employ periodic self-test, to increase test reliability and to reduce test cost. This paper analyzes leakage current and presents a kind of leakage current simulator based on the transistor stacking effect.Using it, we propose techniques based on don't care bits (denoted by Xs) in test vectors to optimize leakage current in integrated circuit (IC) test by genetic algorithm. The techniques identify a set of don't care inputs in given test vectors and reassign specified logic values to the X inputs by the genetic algorithm to get minimum leakage vector (MLV).Experimental results indicate that the techniques can effectually optimize leakage current of combinational circuits and sequential circuits during test while maintaining high fault coverage.

  10. Generation and analysis of expressed sequence tags from six developing xylem libraries in Pinus radiata D. Don

    Directory of Open Access Journals (Sweden)

    Dillon Shannon K

    2009-01-01

    Full Text Available Abstract Background Wood is a major renewable natural resource for the timber, fibre and bioenergy industry. Pinus radiata D. Don is the most important commercial plantation tree species in Australia and several other countries; however, genomic resources for this species are very limited in public databases. Our primary objective was to sequence a large number of expressed sequence tags (ESTs from genes involved in wood formation in radiata pine. Results Six developing xylem cDNA libraries were constructed from earlywood and latewood tissues sampled at juvenile (7 yrs, transition (11 yrs and mature (30 yrs ages, respectively. These xylem tissues represent six typical development stages in a rotation period of radiata pine. A total of 6,389 high quality ESTs were collected from 5,952 cDNA clones. Assembly of 5,952 ESTs from 5' end sequences generated 3,304 unigenes including 952 contigs and 2,352 singletons. About 97.0% of the 5,952 ESTs and 96.1% of the unigenes have matches in the UniProt and TIGR databases. Of the 3,174 unigenes with matches, 42.9% were not assigned GO (Gene Ontology terms and their functions are unknown or unclassified. More than half (52.1% of the 5,952 ESTs have matches in the Pfam database and represent 772 known protein families. About 18.0% of the 5,952 ESTs matched cell wall related genes in the MAIZEWALL database, representing all 18 categories, 91 of all 174 families and possibly 557 genes. Fifteen cell wall-related genes are ranked in the 30 most abundant genes, including CesA, tubulin, AGP, SAMS, actin, laccase, CCoAMT, MetE, phytocyanin, pectate lyase, cellulase, SuSy, expansin, chitinase and UDP-glucose dehydrogenase. Based on the PlantTFDB database 41 of the 64 transcription factor families in the poplar genome were identified as being involved in radiata pine wood formation. Comparative analysis of GO term abundance revealed a distinct transcriptome in juvenile earlywood formation compared to other stages of

  11. Beat the Odds...Why Some Schools With Latino Children Beat the Odds and Others Don't

    Science.gov (United States)

    Waits, Mary Jo; Campbell, Heather E.; Gau, Rebecca; Jacobs, Ellen; Rex, Tom; Hess, Robert K.

    2006-01-01

    Using the methodology of Jim Collins from his book "Good to Great: Why Some Companies Make the Leap...and Other's Don't," the authors of this report, under the sponsorship of the Center for the Future of Arizona and the Morrison Institute, found 12 elementary and middle schools in Arizona--schools whose students are mostly Latino and mostly…

  12. Social and Political Activization of the Don Nobility in the Early 20th Century and “Noble Liberalism”

    Directory of Open Access Journals (Sweden)

    Bratolyubova Mariya Viktorovna

    2014-03-01

    Full Text Available Peculiarity of the social, economic and legal status of the Don nobility influenced its political alertness and the opposition character of activity. The liberal wing supported the creation of a new world outlook system which was expected to provide the basis for transforming the political system by means of gradual transition to people’s representatives or constitutional form of government. The liberal landowners insisted on the introduction of zemstvo (district council, the solution of the Cossack issue; they strived for liberalization of the military and bureaucratic control of the Cossack troops and demanded the withdrawal of civil government agencies from the competence of the Military department. The liberal nobility actively supported the change of conditions of service for the Cossacks and pressed for easier draft obligation and releasing the Cossack troops from police duties which clashed with the military dignity. The conservative type of liberalism prevailed within the noble class of the DHR that distanced itself from both the left and the right political forces. The liberalism of the Don nobles was also a reflection of social and cultural peculiarities of the Cossacks. The social basis of the Don liberalism differed from the common Russian analogs. As the Cossack class was rather numerous at the Don, and its representatives made up quite an important part of the regional liberal corporation, they influenced its tactics and the political programming considerably.

  13. Antioxidant and anticholinesterase investigations of Rumex hastatus D. Don: potential effectiveness in oxidative stress and neurological disorders

    OpenAIRE

    2015-01-01

    Background Rumex species are traditionally used for the treatment of neurological disorders including headache, migraine, depression, paralysis etc. Several species have been scientifically validated for antioxidant and anticholinestrase potentials. This study aims to investigate Rumex hastatus D. Don crude methanolic extract, subsequent fractions, saponins and flavonoids for acetylcholinestrase, butyrylcholinestrase inhibition and diverse antioxidant activities to validate its folkloric uses...

  14. 77 FR 23673 - Notice of Stakeholder Meeting: Industry Roundtable-DON/USDA/DOE/DOT-FAA Advanced Drop-In Biofuels...

    Science.gov (United States)

    2012-04-20

    ... From the Federal Register Online via the Government Publishing Office DEPARTMENT OF DEFENSE Department of the Navy Notice of Stakeholder Meeting: Industry Roundtable--DON/USDA/DOE/ DOT-FAA Advanced.... Federal government representatives will also be able to hear from stakeholders as to their abilities...

  15. Don't Call Them Dropouts: Understanding the Experiences of Young People Who Leave High School before Graduation

    Science.gov (United States)

    Hynes, Michelle

    2014-01-01

    "Don't Call Them Dropouts" adds to the large and growing body of research about why some young people fail to complete high school on the traditional four-year timeline. While a high school diploma is only a starting line for adult success, it has become increasingly clear that it is crucial for taking the next steps in college and…

  16. 77 FR 28895 - Don Edwards San Francisco Bay National Wildlife Refuge, Alameda, Santa Clara, and San Mateo...

    Science.gov (United States)

    2012-05-16

    ... Fish and Wildlife Service Don Edwards San Francisco Bay National Wildlife Refuge, Alameda, Santa Clara... Wildlife Refuge, located in the Alameda, Santa Clara, and San Mateo Counties of California, consists of... Federal Register notice of intent on February 23, 2010 (75 FR 8106), to solicit additional comments....

  17. 78 FR 21397 - Don Edwards San Francisco Bay National Wildlife Refuge, Alameda, Santa Clara, and San Mateo...

    Science.gov (United States)

    2013-04-10

    ... Fish and Wildlife Service Don Edwards San Francisco Bay National Wildlife Refuge, Alameda, Santa Clara... Alameda, Santa Clara, and San Mateo Counties of California, consists of several noncontiguous parcels... notice (77 FR 28895, May 16, 2012). The Draft CCP/EA identified and evaluated three alternatives...

  18. 75 FR 8106 - Don Edwards San Francisco Bay National Wildlife Refuge, Alameda, Santa Clara, and San Mateo...

    Science.gov (United States)

    2010-02-23

    ... Fish and Wildlife Service Don Edwards San Francisco Bay National Wildlife Refuge, Alameda, Santa Clara... located in Alameda, Santa Clara, and San Mateo Counties of California. We provide this notice in... in Alameda, Santa Clara, and San Mateo Counties of California, consists of several non...

  19. Las Voces de la Tradicion en "Don Segundo Sombra" a la Luz de la Reflexion Estilistica de Amado Alonso

    OpenAIRE

    Martos Nuñez, Eloy

    1995-01-01

    SE EXPLICA EL PROBLEMA DE LA SINGULARIDAD ESTILISTICA DE DON SEGUNDO SOMBRA, A PARTIR DEL ANALISIS DE AMADO ALONSO. GÜIRALDES OPTO POR LA FORMULA DE UNAS MEMORIAS Y POR CREAR UN LENGUAJE NUEVO QUE INTEGRASE LA RICA PERSPECTIVA DE LA TRADICION Y SUS NARRADORES, Y SU PROPIA VISION PLASTICA Y LITERARIA.

  20. Get Thee Away, Knight, Be Gone, Cavalier: English Translations of the Biscayan Squire Episode in "Don Quixote de la Mancha"

    Science.gov (United States)

    Azevedo, Milton M.

    2009-01-01

    This article analyzes twelve English translations of the Biscayan squire's speech ("Don Quijote de la Mancha", Chapter Eight) published between 1612 and 2000. The solutions found fall into three categories, namely (a) using standard English and risking misrepresenting the style of the original and omitting relevant cultural connotations, (b) using…

  1. Sarmatian Monuments in the Forest-Steppe Interfluve Area Between the Don and the Volga Rivers (Zoning Experience

    Directory of Open Access Journals (Sweden)

    Berestnev Roman Sergeevich

    2015-06-01

    Full Text Available The article is devoted to the zoning of Sarmatian antiquities in the forest-steppe interfluve area between the Don and the Volga rivers. On the basis of the analysis, the authors allocate four areas of burial monuments in the Northern periphery of the Asian Sarmatia (the river in Voronezh, the Middle Don, the Upper Don, the area between the Khoper and the Volga rivers. The stages of the forest-steppe area development by the Sarmatians in the Don River, the Khoper river and the right bank of the Volga river were also studied. The first stage is characterized by the general description of Sarmatian monuments located in the forest-steppe area between the Volga and the Khoper. The authors carry out the detailed review of the distinctive features of the funeral rites and some peculiar details: stratigraphy, structures beneath barrows, forms of burial pits, postures of the deceased, orientation, use of fire, imported and domestic utensils, weapons and ornaments. Archaeological materials are compared with the data of ancient sources (Claudius Ptolemy. The result of the study consists in the comparative analysis of the binding of specific ethnic groups in the allocated areas. The authors attribute the Sarmatians on the Voronezh river with the Gippofagi ancient authors; the Aseyes and the Sakata – with the area of monuments in the forest between the Volga and the Khoper.

  2. Un sabio médico gallego: don Jacobo López Elizagaray (1860-1934

    Directory of Open Access Journals (Sweden)

    Ponte Hernando, Fernando J.

    2013-12-01

    Full Text Available Biographical Portrait of don Jacobo López Elizagaray, one of the most prominent figures of Compostelan Medical School accredited in Madrid. True master of Internal Medicine of the last third of the nineteenth and twentieth first, pioneer in the modernization of Spanish medicine, was teacher and fellow of extraordinarily distinguished medical personalities from Galicia and from all Spain, as Drs. Varela y Varela, Santiago Carro García, Nóvoa Santos Gómez Ulla, Marañón, Jimenez Diaz, Goyanes Capdevila, Varela Radio and many others.Retrato biográfico de don Jacobo López Elizagaray, una de las más destacadas figuras de la Escuela Médica Compostelana acreditada en Madrid. Auténtico maestro de la Medicina interna del último tercio del siglo XIX y primero del XX, pionero en la modernización de la Medicina española, fue maestro y compañero de destacadísimas personalidades médicas gallegas y de toda España, como los Dres. Varela y Varela, Santiago Carro García, Nóvoa Santos, Gómez Ulla, Marañón, Jiménez Díaz, Goyanes Capdevila, Varela Radío y muchos otros. [gl] Retrato biográfico de don Jacobo López Elizagaray, unha das máis destacadas figuras da Escola Médica Compostelana acreditada en Madrid. Auténtico mestre da Medicina Interna do último tercio de século XIX e primeiro do XX, pioneiro na modernización da Medicina española, foi mestre e compañeiro de moi salientables personalidades médicas galegas e de toda España, como os Dres. Varela e Varela, Santiago Carro García, Nóvoa Santos, Gómez Ulla, Marañón, Jiménez Díaz, Goyanes Capdevila, Varela Radío e moitos outros.

  3. Defining Hair Follicles in the Age of Stem Cell Bioengineering

    OpenAIRE

    Chuong, Cheng-Ming; Cotsarelis, George; Stenn, Kurt

    2007-01-01

    One challenge faced by stem cell biologists is the bioengineering of an organ. Ehama et al. (2007, this issue) used cells derived from human and rodent epidermis and dermal papilla to reconstitute hair-follicle mini-organs. Some result in hair follicles; others are hair follicle–like. The challenge calls for the development of a set of criteria to define a hair follicle so that bioengineered products in the future can be evaluated.

  4. Fate of Fusarium mycotoxins in the cereal product supply chain: the deoxynivalenol (DON) case within industrial bread-making technology.

    Science.gov (United States)

    Bergamini, E; Catellani, D; Dall'asta, C; Galaverna, G; Dossena, A; Marchelli, R; Suman, M

    2010-05-01

    Fusarium mycotoxins are a relevant problem in the cereal supply chain at a worldwide level, with wheat, maize and barley being the main contaminated crops. Mould growth can happen in the pre-harvest phase and also during transport and storage due to ineffective drying conditions. Among Fusarium toxins, deoxynivalenol (DON) is considered the most important contaminant in wheat due to its widespread occurrence. In the last years the European Food Safety Authority (EFSA) and the European Commission have frequently expressed opinions on Fusarium toxins, setting limits, regulations and guidelines in order to reduce their levels in raw materials and food commodities. In particular, European legislation (Reg. 1881/2006) sets the maximum limit for DON in flour and bread as 750 and 500 microg kg(-1) respectively. Relatively few studies have taken into account the loss of trichothecenes during processing, focusing on how processing factors may influence their degradation. In particular, the description of DON behaviour during bread-making is very difficult, since complex physico-chemical modifications occur during the transformation of the raw ingredients into the final product. In the present study, we studied how DON concentration may be influenced by modifying bread-making parameters, with a special emphasis on the fermentation and baking stages, starting from a naturally contaminated flour at both pilot and industrial scales. Exploiting the power of a Design of Experiments (DoE) approach to consider the great complexity of the studied system, the obtained model shows satisfying goodness-of-fit and prediction, suggesting that the baking step (time/temperature ranges) is crucial for minimizing native DON level in bread.

  5. Dons quotidiens dans les espaces sacrés de la zawya

    OpenAIRE

    Naji, Salima

    2012-01-01

    L’office du Saint, anwal, est un vaste espace composé de plusieurs salles dont l’une possède un foyer allumé tous les matins. L’eau de pluie, don de Dieu recueilli par les nombreuses citernes qui entourent l’édifice, est portée à ébullition dans d’imposants chaudrons faisant couscoussiers, pour ces bontés systématiquement servies aux pèlerins. En effet, cette eau réputée pour ses qualités purificatrices, accompagne une assiette de couscous d’orge brut très salé, offerts à l’hôte de passage, c...

  6. War as Haiku: The Politics of Don DeLillo’s Late Style

    Directory of Open Access Journals (Sweden)

    Matthew Shipe

    2016-05-01

    Full Text Available In the wake of 'Underworld' (1997, Don DeLillo’s work has been cast largely in a minor key. In this essay, I will focus on 'Point Omega', the work that I believe most clearly illuminates the political implications of DeLillo’s late style as it tackles the consequences of the wars in Afghanistan and Iraq. More broadly, I hope to connect 'Point Omega' to the work of DeLillo’s contemporaries that have also speculated on the historical and political implications of the War on Terror. Centering on 'Point Omega', I am interested in how DeLillo has adopted a distinctive narrative form in his most recent fiction that frequently unsettles and complicates the historical narratives that he had established in his earlier work.

  7. What We Don't Understand, We Explain to Each Other

    Science.gov (United States)

    Pines, David

    2015-12-01

    "What we don't understand, we explain to each other" was Robert Oppenheimer's 1948 description of theoretical physics as a profession. Because the phrase connects research, teaching, and learning, it seemed the right approach for the talk I gave to the AAPT on receiving the 2013 J.D. Jackson Award for Excellence in Graduate Teaching, on which the present article is based. In what follows I describe how my experiences as a student, researcher, and teacher combined to shape the way I learn and teach physics, from my own courses to inventing and editing Frontiers in Physics, reshaping Reviews of Modern Physics, and experimenting with different approaches to communicating key concepts in physics.

  8. Compte rendu de "Les enfants de Don Quichotte", P. Bruneeaux, Presses Universitaires de Vincennes, 2013

    OpenAIRE

    Frère, Bruno

    2014-01-01

    Les enfants de Don Quichotte est un ouvrage qui présente intérêt non négligeable. Il s’agit de l’une des rares études sociologiques (la seule ?) portant sur un mouvement qui pourtant fit grand bruit dans le monde médiatique et politique il y a quelques années : l’occupation des abords du canal Saint Martin par des SDF regroupés sous tentes. C’est délibérément que les auteurs de ce collectif ont choisi l’optique de la sociologie critique bourdieusienne pour enquêter. À ce titre, le parti pris ...

  9. [Genetic structure of the populations of Pallas pine (Pinus pallasiana D. Don) reforested in extreme conditions].

    Science.gov (United States)

    Korshikov, I I; Krasnoshtan, O V

    2010-01-01

    The paper reports on an intensive reforestation of Pallas pine (Pinus pallasiana D. Don) in post-fire sites in the native Mountainous Crimean populations and around the previously planted seed-producing trees in ore-mining dumps of the Krivoy Rog region. Self-sown progeny growing in the dump is characterized by a better growth and comes to the reproductive development phase earlier compared to that one growing in post-fire forest. Allele variability at 20 allozyme loci is less in self-sown progeny than in the native populations whereas its heterozygosity level is similar. Genetic distance (D(N)) among self-sown progenies in post-fire sites and ore-mining dump is comparable to that of the natural populations. PMID:20608157

  10. The Secret List of Dos and Don'ts for Filmmaking

    Science.gov (United States)

    Kramer, N.

    2012-12-01

    Science is a massive black box to billions of people who walk the streets. However, the process of filmmaking can be equally as mystifying. As with the development of many scientific experiments, the process starts on a napkin at a restaurant…but then what? The road to scientific publication is propelled by a canonical list of several dos and don't that fit most situations. An equally useful list exists for up-and-coming producers. The list streamlines efforts, optimizes your use of the tools at your fingertips and enhances impact. Many fundamentals can be learned from books, but during this talk we will project and discuss several examples of best practices, from honing a story, to identifying audience appeal, filming, editing and the secrets of inexpensively acquiring expert help. Whether your goal is a two-minute webisode or a 90 minute documentary, these time-tested practices, with a little awareness, can give life to your films.;

  11. El sistema preventivo de Don Bosco en la escuela del siglo XXI

    OpenAIRE

    Navajas-Josa, Alejandro

    2014-01-01

    Este trabajo pretende abordar el método preventivo de don Bosco (1815-1888), sacerdote y educador italiano, fundador de los salesianos, congregación religiosa que cuenta en la actualidad con casi 16.000 sacerdotes que operan en más de 130 países del mundo. En 1877, publicó un opúsculo donde recogía las líneas maestras de su metodología, que se sigue aplicando en los miles de colegios y centros de formación profesional de los salesianos. A lo largo de estas páginas se demuestra cómo este siste...

  12. Una figura funeraria infantil de madera en el Instituto Valencia de Don Juan

    Directory of Open Access Journals (Sweden)

    Fernández Ahijado, Carmen

    2003-09-01

    Full Text Available Entre las piezas que componen la colección de escultura del Instituto Valencia de don Juan encontramos la estatua orante de un niño, realizada en madera, tela encolada y yeso. Sus medidas son 77,5 cm de alto, 38 cm de ancho y 38,5 cm de fondo. Presenta elegancia señorial, carácter y vitalidad en su cabeza. Aparece ante nuestros ojos con los mismos ademanes y la misma figura rígida y envarada que los adultos. La actitud recogida junto con su sencilla vestimenta acentúan el intimismo religioso del orante. Se consideró que era interesante encontrar otros ejemplos de este tipo de escultura funeraria infantil en madera, material poco usado en este género debido a su propia naturaleza. La madera se asociaba a transitoriedad…

  13. El político Don Fernando El Católico

    OpenAIRE

    Montaner Frutos, Alberto

    2001-01-01

    En la obra "El político Don Fernando El Católico" Baltasar Gracián cifra una reflexión política de hondo calado en un tomito en dieciseisavo que vio por primera vez la luz en 1640. En esta obra podemos encontra además de los problemas bibliográficos y ecdóticos, tres aspectos fundamentales podríamos considerar en el análisis de El Político: el estilístico, el histórico cuyo contenido desborda la fundamental referencia a Fernando el Católico que campea ya desde la portada; y el propia...

  14. NH4NO3 fertilizer induces DON leaching from Sphagnum magellanicum

    Science.gov (United States)

    Hartsock, J. A.; Vile, M. A.; Wieder, R.

    2011-12-01

    Boreal peatlands globally function as long-term sinks for atmosheric CO2. Mosses in the genus Sphagnum dominate the understory of boreal peatlands, especially in ombrotrophic bogs for which the sole sources of new nitrogen (N) is atmospheric deposition and biological N2-fixation. Sphagnum mosses in undisturbed regions generally are thought to be N-limited because of slow nutrient turnover and low rates of atmospheric N deposition. In Alberta, Canada, mean background N deposition rates are less than 1 kg N ha-1yr-1, but increasing atmospheric N deposition is occurring as a consequence of oil sands development in northern Alberta. In some European countries, anthropogenic practices have increased annual N deposition rates to levels in excess of 20 kg N ha-1yr-1. Increased rates of atmospheric N deposition have been shown to alter N cycling processes of Sphagnum mosses. Current hypotheses propose that Sphagnum mosses leach dissolved organic nitrogen (DON) from their tissues to mitigate N saturation stress in high N deposition areas. The goal of this study was to determine whether Sphagnum magellanicum leaches DON when exposed to elevated concentrations of NH4NO3 applied experimentally as synthetic rainfall. Sphagnum magellanicum was collected from a fen in Driftwood, Pennsylvania (41°13'58"N 78°11'44"W). Intact cores (10.5cm x 5.5cm) were grown in 500 mL culture flasks in a controlled environment chamber at Villanova University. Ammonium nitrate was applied to moss treatments eight times over 73 days at concentrations equivalent to 6 kg N ha-1yr-1, 12 kg N ha-1yr-1, and 18 kg N ha-1yr-1 (n=5 replicates per treatment, 400 mL H2O per N addition). Leachates (freely drained water samples) were analyzed for dissolved total nitrogen (TN), ammonium (NH4+-N), nitrate (NO3--N), and DON (TN-DIN) concentrations. Results show that after 73 days, increasing DIN resulted in: 1) a shift from net N mineralization to net NH4+ immobilization (Control: 376.3 ± 15.2 ug/g; High N: -377

  15. "Gathering Facts for the End of the World": Don DeLillo's Archive of Global Turbulence

    Directory of Open Access Journals (Sweden)

    Cornelius Collins

    2016-05-01

    Full Text Available Over his career, Don DeLillo grows to inhabit more complex and critical ideas about politics, capitalism, and strategies for opposition than the remote affect of his earlier work might suggest. As part of this project, he developed an advanced analysis of conditions under the global finance regimes gaining strength in the West. The author's papers at the Harry Ransom Center show that he arrived at this informed position through a program of independent research, his newspaper cuttings from this period vividly demonstrating reflections on the meaning of US global power. Once back in New York, his investment in public intellectual debates on the meaning of "terrorism" led to a characteristic but problematic emphasis on free speech as a political issue. More recently, his work has turned to sophisticated theoretical texts for increasingly subtle ideas about globalization and its opponents.

  16. Analysis of Several Popular Cultivars of Madagascar Periwinkle (Catharanthus roseus (L. G. Don. using Biochemical Markers

    Directory of Open Access Journals (Sweden)

    Owk ANIEL KUMAR

    2013-12-01

    Full Text Available Band designs of esterase (EST, peroxidase (PO and polyphenol oxidase (PPO isozymes in several selected cultivars of Catharanthus roseus by using native polyacrylamide gel electrophoresis (PAGE were investigated in this study. It was confirmed that cultivar differences in isozyme polymorphism can be revealed by applied electrophoretic patterns. Three isozyme systems produced a total of 16 bands with polymorphism ranged from 66.6-100%. Considering the patterns of isozyme variations in the five cultivars of Catharanthus roseus, it is evident that the cultivar ‘First kiss coral’ displayed crimson red petal with large white eye’ displayed demarked profiles of EST, PO and PPO isozymes than other cultivars. This is the first report on isozyme polymorphism in members of the Cathanarathus roseus (L. G. Don.

  17. Orthodox Revival in the Lower Volga Area and the Don in 1942–1943

    Directory of Open Access Journals (Sweden)

    Mordvinov Sergey Valeryevich

    2013-02-01

    Full Text Available The paper shows the impact of the Battle of Stalingrad in the revival of Orthodoxy in the Lower Volga and the Don, a comparison of the reconstruction process in the dioceses of the region; its characteristics are revealed in Stalingrad, Saratov, Astrakhan and Rostov regions. The author comes to the conclusion that the battle of Stalingrad was the catalyst recovery Orthodoxy in the province. The pace of discovery of temples was higher in the occupied areas of the Rostov and Stalingrad areas than in front-line areas. Imbalance in the number of active parishes continued to persist after the end of the Battle of Stalingrad. Church patriotic charity in 1942–1943 was the most active in the Saratov and Stalingrad diocese.

  18. Effects of feeding deoxynivalenol (DON)-contaminated wheat to laying hens and roosters of different genetic background on the reproductive performance and health of the newly hatched chicks.

    Science.gov (United States)

    Ebrahem, Mohammad; Kersten, Susanne; Valenta, Hana; Breves, Gerhard; Beineke, Andreas; Hermeyer, Kathrin; Dänicke, Sven

    2014-08-01

    A total of 216 23-week-old laying hens from two different genetic backgrounds (half of the birds were Lohmann brown [LB] and [LSL] hens, respectively) and 24 adult roosters were assigned to a feeding trial to study the effect of increasing concentrations of deoxynivalenol (DON) in the diet (0, 5, 10 mg/kg) on the reproductive performance of hens and roosters, and the health of the newly hatched chicks. Hatchability was adversely affected by the presence of DON in LB hens' diet, while the hatchability of the LSL chicks was significantly higher than LB chicks. An interaction effect between DON in the hens' diet and the breed was noticed on fertility, as the fertility was decreased in the eggs of LB hens receiving 10 mg/kg DON in their diet and increased in the eggs of LSL hens fed 10 mg/kg DON. Moreover, spleen relative weight was significantly decreased in the chicks hatched from eggs of hens fed contaminated diets, while gizzard relative weight was significantly decreased in LB chicks with 10 mg/kg DON in their diet compared with the control group. On the other hand, the chicks' haematology and organ histopathology were not affected by the dietary treatment. Additionally, the presence of DON in the roosters' diet had no effect on fertility (the percentage of fertile eggs of all laid eggs). Consequently, the current results indicate a negative impact of DON in LB hens' diet on fertility and hatchability, indicating that the breed of the hens seems to be an additional factor influencing the effect of DON on reproductive performance of the laying hens.

  19. Size, Albedo, and Taxonomy of the Don Quijote Space Mission Target

    Science.gov (United States)

    Harris, Alan; Mueller, Michael; Fitzsimmons, Alan

    2006-03-01

    Rendezvous and lander missions are a very effective but very expensive way of investigating Solar-System bodies. The planning, optimization and success of space missions depends crucially on prior remotely-sensed knowledge of target bodies. Near-Earth asteroids (NEAs), which are mainly fragments of main-belt asteroids, are seen as important goals for investigation by space missions, mainly due to the role their forebears played in planet formation and the evolution of the Solar System, but also for the pragmatic reason that these objects can collide with the Earth with potentially devastating consequences. The European Space Agency is currently planning the Don Quijote mission to a NEA, which includes a rendezvous (and perhaps a lander) spacecraft and an impactor vehicle. The aim is to study the physical properties of the target asteroid and the effects of the impact on its dynamical state, as a first step in considering realistic mitigation measures against an eventual hazardous NEA. Two potential targets have been selected for the mission, the preferred one being (10302) 1989 ML, which is energetically easier to reach and is possibly a scientifically interesting primitive asteroid. However, due to the ambiguity of available spectral data, it is currently not possible to confidently determine the taxonomic type and mineralogy of this object. Crucially, the albedo is uncertain by a factor of 10, which leads to large uncertainties in the size and mass and hence the planned near-surface operations of Don Quijote. Thermal-infrared observations are urgently required for accurate size and albedo determination. These observations, which can only be carried out by Spitzer and would require only a modest amount of observing time, would enable an accurate diameter to be derived for the first time and the resulting albedo would remove the taxonomic ambiguity. The proposed Spitzer observations are critical for effective mission planning and would greatly increase our

  20. SOCIAL MOVEMENTS OF VOLGA-DON REGION IN THE FIRST RUSSIAN REVOLUTION (1905-1907

    Directory of Open Access Journals (Sweden)

    N. F. Bolotov

    2015-01-01

    Full Text Available The article is an attempt to analyze and provide scientific assessment of the events of the political history of Russia in the early XX century, whiсh took place, in the opinion of the authors, under influence by a number of objective factors. The regional specific of the article (in the example of the southern districts of the Saratov province and Cossack areas of the Volga-Don interfluve aims to search for the general laws of the formation of social and political movements that aim to change the existing system in Russia during the First Russian Revolution. The authors attempt to systematize the vast amount of material, which allows to identify the main causes of the mass revolutionary movement, character and social composition of the political groups and their impact on changes in the socio-political life of the region. Particular attention is paid to the activities of the Russian Social-Democratic Party, which position was strengthened by difficult economic plight of the workers and repressive government policies aimed at curbing the revolutionary movement. In modern conditions of social and political instability in many regions of the world, provoked by political technologies of "orange" revolution, it is highly relevant to study history of the First Russian Revolution of 1905-1907, which the 110th anniversary passed unnoticed. Despite the defeat, the revolution was of great importance for the emergence of civil society in Russia. As a result of mass protests of various social strata of Russian society, the first steps towards the formation of constitutionalism and parliamentarianism in Russia were made. In predominantly peasant Volga-Don region with high industrialized city Tsaritsyn all the contradictions of feudal-capitalist Russia's reality were focused and were clearly manifested, that turned into a powerful social explosion, which became the preparation of the 1917 Revolution.

  1. Peut-on penser le travail par le don ? Débat avec la rédaction de la Nouvelle Revue du Travail

    OpenAIRE

    Caillé, Alain; Alter, Norbert

    2012-01-01

    Dans un échange avec la Nouvelle Revue du Travail, Alain Caillé et Norbert Alter soutiennent leur lecture maussienne des réalités contemporaines du travail en discutant les questions centrales du capitalisme, du salariat, de l’entreprise et du travail sociologique. Cet échange fait suite aux contributions d’Alain Caillé et Norbert Alter dans ce Chorus « Peut-on penser le travail par le don ? De la mise en œuvre du paradigme du don contre-don en sociologie du travail. »

  2. Synthetic Biology Outside the Cell: Linking Computational Tools to Cell-Free Systems

    OpenAIRE

    Lewis, Daniel D.; Villarreal, Fernando D.; Wu, Fan; Tan, Cheemeng

    2014-01-01

    As mathematical models become more commonly integrated into the study of biology, a common language for describing biological processes is manifesting. Many tools have emerged for the simulation of in vivo synthetic biological systems, with only a few examples of prominent work done on predicting the dynamics of cell-free synthetic systems. At the same time, experimental biologists have begun to study dynamics of in vitro systems encapsulated by amphiphilic molecules, opening the door for the...

  3. Fluorescence lifetime imaging microscopy (FLIM) to quantify protein-protein interactions inside cells.

    Science.gov (United States)

    Duncan, R R

    2006-11-01

    Recent developments in cellular imaging spectroscopy now permit the minimally invasive study of protein dynamics inside living cells. These advances are of interest to cell biologists, as proteins rarely act in isolation, but rather in concert with others in forming cellular machinery. Until recently, all protein interactions had to be determined in vitro using biochemical approaches: this biochemical legacy has provided cell biologists with the basis to test defined protein-protein interactions not only inside cells, but now also with high spatial resolution. These techniques can detect and quantify protein behaviours down to the single-molecule level, all inside living cells. More recent developments in TCSPC (time-correlated single-photon counting) imaging are now also driving towards being able to determine protein interaction rates with similar spatial resolution, and together, these experimental advances allow investigators to perform biochemical experiments inside living cells. PMID:17052173

  4. Mechanistic modeling confronts the complexity of molecular cell biology.

    Science.gov (United States)

    Phair, Robert D

    2014-11-01

    Mechanistic modeling has the potential to transform how cell biologists contend with the inescapable complexity of modern biology. I am a physiologist-electrical engineer-systems biologist who has been working at the level of cell biology for the past 24 years. This perspective aims 1) to convey why we build models, 2) to enumerate the major approaches to modeling and their philosophical differences, 3) to address some recurrent concerns raised by experimentalists, and then 4) to imagine a future in which teams of experimentalists and modelers build-and subject to exhaustive experimental tests-models covering the entire spectrum from molecular cell biology to human pathophysiology. There is, in my view, no technical obstacle to this future, but it will require some plasticity in the biological research mind-set.

  5. Cell biology as the basis of a better understanding of cancer

    OpenAIRE

    Wheatley Denys N

    2005-01-01

    Abstract Clinicians will argue that cancer can only really receive the treatment that is needed through thorough understanding of medicine. However, even empirical approaches to therapy result in experimental analysis of the agencies involved on test cells, usually in culture. From the obverse perspective, cell biologists will argue that until we fully understand cell cycle regulation, tumour management will be too imprecise to make the best advances. A forum is needed whereby the fundamental...

  6. Omnis cellula e cellula revisited: cell biology as the foundation of pathology.

    Science.gov (United States)

    Wright, Nicholas A; Poulsom, Richard

    2012-01-01

    This 2012 Annual Review Issue of The Journal of Pathology argues strongly that cell biology, in its many disciplines, underpins the foundation of our understanding of the mechanisms of disease-the holy grail of pathology. Our increasing knowledge of the human genome will not be enough to attain this goal without parallel developments in our comprehension of the results, at the cellular level, of these genetic changes. In the end, it is cell biology and cell biologists who will deliver this mission.

  7. Amendment to refuge hunting plan for waterfowl on 5,500 acres of former commercial salt ponds : Don Edwards San Francisco Bay NWR

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This amendment to the 1982 Don Edwards San Francisco Bay NWR waterfowl hunting plan was written to address 5,500 acres of salt pond recently acquisitioned by the...

  8. On the "I don't think..." and "I think...not..." constructions%"I don't think..."和"I think...not..."的句式分析

    Institute of Scientific and Technical Information of China (English)

    方立

    2002-01-01

    本文探讨"I don't think..."和"I think...not…"这两个句式的句法、语义和语用,以及可用于这两个句式的词类.分析表明,此句式可用否定词提升转换规则解释,但该规则只适用于非事实谓词的一个小类.

  9. Studying cell biology in the skin

    Science.gov (United States)

    Morrow, Angel; Lechler, Terry

    2015-01-01

    Advances in cell biology have often been driven by studies in diverse organisms and cell types. Although there are technical reasons for why different cell types are used, there are also important physiological reasons. For example, ultrastructural studies of vesicle transport were aided by the use of professional secretory cell types. The use of tissues/primary cells has the advantage not only of using cells that are adapted to the use of certain cell biological machinery, but also of highlighting the physiological roles of this machinery. Here we discuss advantages of the skin as a model system. We discuss both advances in cell biology that used the skin as a driving force and future prospects for use of the skin to understand basic cell biology. A unique combination of characteristics and tools makes the skin a useful in vivo model system for many cell biologists. PMID:26564861

  10. Studying cell biology in the skin.

    Science.gov (United States)

    Morrow, Angel; Lechler, Terry

    2015-11-15

    Advances in cell biology have often been driven by studies in diverse organisms and cell types. Although there are technical reasons for why different cell types are used, there are also important physiological reasons. For example, ultrastructural studies of vesicle transport were aided by the use of professional secretory cell types. The use of tissues/primary cells has the advantage not only of using cells that are adapted to the use of certain cell biological machinery, but also of highlighting the physiological roles of this machinery. Here we discuss advantages of the skin as a model system. We discuss both advances in cell biology that used the skin as a driving force and future prospects for use of the skin to understand basic cell biology. A unique combination of characteristics and tools makes the skin a useful in vivo model system for many cell biologists.

  11. MENINGKATKAN MOTIVASI DAN PRESTASI BELAJAR MATEMATIKA SISWA KELAS V A SD FRATER DON BOSCO TARAKAN MENGGUNAKAN MODEL PEMBELAJARAN KOOPERATIF TIPE TEAM ASISTED INDIVIDUALIZATION TAHUN PELAJARAN 2010/2011

    OpenAIRE

    Agustinus Toding Bua

    2011-01-01

    "Agustinus Toding Bua. Meningkatkan Motivasi dan Prestasi Belajar Siswa Kelas V A SD Frater Don Bosco Tarakan Menggunakan Model Pembelajaran Kooperatif Tipe TAI Tahun Pelajaran 2010/2011 (dibimbing oleh Samsudin dan Jero). Tujuan Penelitian Untuk meningkatkan motivasi dan prestasi belajar matematika siswa kelas V A SD frater Don Bosco Tarakan pada materi mengidentifikasi sifat-sifat bangun datar dan bangun ruang. Pengumpulan data diperoleh dari hasil observasi, angket motivasi, dan pos tes p...

  12. DMPD: How Toll-like receptors signal: what we know and what we don't know. [Dynamic Macrophage Pathway CSML Database

    Lifescience Database Archive (English)

    Full Text Available 16343886 How Toll-like receptors signal: what we know and what we don't know. O'Nei...ll LA. Curr Opin Immunol. 2006 Feb;18(1):3-9. Epub 2005 Dec 15. (.png) (.svg) (.html) (.csml) Show How Toll-...like receptors signal: what we know and what we don't know. PubmedID 16343886 Title How Toll-like receptors

  13. Effects of Pinus flexilis on the dynamics and structure of plant communities on the northern Rocky Mountain front, and, Training biologists for emerging niches in non-traditional jobs

    Science.gov (United States)

    Baumeister, Dayna Marie

    been characterized by invasion of P. flexilis and subsequent shifts in understory species composition apparently facilitated by P. flexilis. Lastly, I examined the role of the emerging science of biomimicry in providing employment for biologists. Biomimicry as a discipline seeks design solutions inspired by nature. Industries employing this tool require biologists with an understanding of biology from a functional perspective, good communication skills, and a background in applied natural history.

  14. Karakterisasi Simplisia Dan Skrining Fitokimia Serta Isolasi Alkaloida Dari Ekstrak Metanol Daun Kitolod (Hippobroma Longiflora (L.) G. Don)

    OpenAIRE

    Lubis, Suci Lestari

    2015-01-01

    Kitolod (Hippobroma longiflora (L.) G. Don) is an aromatic plant of the family Campanulaceae. The purpose of this research was to determine the simplex characteristics, and to determine the group of chemical compounds contained in kitolod leaves and the isolation of alkaloidal compounds. The isolates were characterized by ultraviolet (UV) and infrared (IR) spectrophotometries. This research included simplex characterization, phytochemical screening, extraction by percolation with methanol...

  15. Proteomic profiling of proteins associated with the rejuvenation of Sequoia sempervirens (D. Don) Endl

    OpenAIRE

    Chen Yu-Ting; Kuo Ching-I; Huang Bau-Lian; Hsu Ya-Wen; Hsieh Tsung-Ju; Shen Chin-Hui; Chen Peng-Jen; Chang Ing-Feng; Chu Hsiu-An; Yeh Kai-Wun; Huang Li-Chun

    2010-01-01

    Abstract Background Restoration of rooting competence is important for rejuvenation in Sequoia sempervirens (D. Don) Endl and is achieved by repeatedly grafting Sequoia shoots after 16 and 30 years of cultivation in vitro. Results Mass spectrometry-based proteomic analysis revealed three proteins that differentially accumulated in different rejuvenation stages, including oxygen-evolving enhancer protein 2 (OEE2), glycine-rich RNA-binding protein (RNP), and a thaumatin-like protein. OEE2 was f...

  16. CHARACTER AND FORMS OF INDUSTRIAL GOODS DISTRIBUTION ON DON AND THE NORTH CAUCASUS IN THE GREAT PATRIOTIC WAR DAYS

    Directory of Open Access Journals (Sweden)

    E. V. PANARINA

    2016-01-01

    Full Text Available This article is devoted to realization of a state policy of distribution of industrial goods on Don and the North Caucasus in days of the Great Patriotic War. The principles of use of a rationing system of distribution of essentials, her differentiated character, preferential categories of consumers providing allocation are considered. Concrete examples of application of coupons at acquisition of clothes, footwear and other goods are given. Conditions of execution of this order by local bodies of Don and the North Caucasus are analyzed; the attention to limitation of the allocated manufactured goods from the centralized funds is paid. Much attention is paid to the measures for research of additional sources of providing the population of Don and the North Caucasus taken from local bodies by essentials. The attention to especially difficult situation with providing the population of the region, owing to occupation consequences is paid. Data on restoration and development of the local industry, cooperative artels, increase in release of goods, necessary for the population, are provided. The role of the trade-union organizations in supply of the most needing workers with clothes, footwear and other manufactured goods reveals. The assessment is given to results of collaboration of local bodies and public organizations in providing the population with manufactured goods, the remaining difficult situation in questions of providing inhabitants of Don and the North Caucasus by essentials throughout the entire period of the Great Patriotic War is noted.

  17. [The routes of helminth egg appearance on the territory of recreational areas and sites of the lower Don River].

    Science.gov (United States)

    Khromenkova, E P

    1993-01-01

    Sanitary and parasitologic problems of recreation use of water bodies and coast lines are still to be studied. The author has examined the routes of invasion at recreation areas by the lower Don river. The river bed depositions and water as well as coastline soil were found contaminated with helminthic eggs. These eggs were brought by sewage water, feces, and surface flow. Effective ecologically justified measures should be developed to prevent it.

  18. Evaluation of phytochemical content, antimicrobial, cytotoxic and antitumor activities of extract from Rumex hastatus D. Don roots

    OpenAIRE

    Sahreen, Sumaira; Khan, Muhammad Rashid; Khan, Rahmat Ali; Hadda, Taibi Ben

    2015-01-01

    Background Being a part of Chinese as well as ayurdic herbal system, roots of Rumex hastatus D. Don (RH) is highly medicinal, used to regulated blood pressure. It is also reported that the plant is diuretic, laxative, tonic, used against microbial skin diseases, bilious complaints and jaundice. The present study is conducted to evaluate phytochemical, antimicrobial, antitumor and cytotoxic activities of extract obtained from R. hastatus roots. Methods RH roots were powdered and extracted with...

  19. Hypoglycaemic Effects of Methanolic Leaf Extract of Catharanthus Roseus (LINN.) G. DON (Apocynaceae) in Normal and Diabetic Mice

    OpenAIRE

    Ojewole, John A O; Adewunmi, Clement O

    2000-01-01

    In Southern Africa, as in many other parts of Africa, Catharanthus roseus (Linn.) G. Don is traditionally used in forklore medicine to treat diabetes and a number of other human ailments. Consequently, the hypoglycaemic effects of methanolic leaf extract of C. roseus have been investigated in streptozotocin (STZ)-induced diabetic (hyperglycaemic) mice as well as in normal (normoglycaemic), STZ-untreated mice. Tolbutamide has been used as reference hypoglycaemic agent. While tolbutamide produc...

  20. MENINGKATKAN HASIL BELAJAR SISWA PADA PEMBELAJARAN IPA MENGGUNAKAN METODE EKSPERIMEN DI KELAS IV B SD FRATER DON BOSCO TARAKAN

    OpenAIRE

    Katarina Gaudentia Radjo

    2011-01-01

    KATARINA GAUDENTIA RADJO. 2011. Meningkatkan Hasil Belajar Siswa pada Pembelajaran IPA Menggunakan Metode Eksperimen di Kelas IV B SD Frater Don Bocso Tarakan. Skripsi. Program Studi Pendidikan Guru Sekolah Dasar Fakultas Keguruan dan Ilmu Pendidikan Universitas Borneo Tarakan. Dimbing oleh: Herdiansya dan Arief Ertha Kusuma. Penelitian ini memakai model Penelitian Tindakan Kelas (PTK) yang dilaksanakan oleh guru untuk memecahkan masalah-masalah pembelajaran yang dihadapi oleh guru dan untuk...

  1. "Don't get sick at sea": An Ecological Study of the Fishermen of Pulau Tuba, Langkawi, Malaysia

    OpenAIRE

    Berger, Morten Mack

    2015-01-01

    Abstract Berger, Morten M. (2015). "Don't get sick at sea": An Ecological Study of the Fishermen of Pulau Tuba, Langkawi, Malaysia. Master's thesis in Social Anthropology. Department of Anthropology, University of Oslo. This study is a project based on a fieldwork of 6-months duration at the island of Pulau Tuba in the Langkawi archipelago outside the north-western coast of Malaysia. I've been using anthropological research methods like participant observation and informal interviews for data...

  2. Fate of Fusarium mycotoxins in cereal product supply chain: the DON case within industrial bread-making technology

    OpenAIRE

    BERGAMINI, Elena; Catellani, Dante; Dall'Asta, Chiara; Galaverna, Gianni; Dossena, Arnaldo; Marchelli, Rosangela; Suman, Michele

    2010-01-01

    Abstract Fusarium mycotoxins are a relevant problem in the cereal supply chain at a worldwide level, being wheat, maize and barley the main contaminated crops; mould growth could happen in the pre-harvest phase and also during transport and storage due to ineffective drying conditions. Among Fusarium toxins, deoxynivalenol (DON) is considered the most important contaminant in wheat, due to its wide-spread occurrence. In the last years the European Food Safety Authority and the Euro...

  3. El arzobispado de Don Gonzalo de Aguilar en Santiago de Compostela (1348-1351

    Directory of Open Access Journals (Sweden)

    Sánchez Sánchez, Xosé M.

    2010-06-01

    Full Text Available In the middle of the 14th century the prelature of Santiago de Compostela is occupied by Don Gonzalo de Aguilar, archbishop transferred from Sigüenza and whose pontificate is extended between 1348 and 1351, when he is transferred to Toledo. He is a figure that remained until now in the complete darkness of the history of the Compostellan church, disappearing in fact, in many cases, of the historical considerations, that they avoided him or simply they did not have noticed of his figure, extending the bordering prelatures (Peter V and Gómez Manrique until eliminate his one. It responds to the completely absence of sources and mentions in the Compostellan archives; in order to palliate this fact, we resorted to the bibliography of other seats and, specially, to the sources of the Vatican Secret Archive, with whose aid we can form one first, new and relatively complete vision of its reign in Santiago.

    A mediados del siglo XIV ocupa el arzobispado de Santiago de Compostela Don Gonzalo de Aguilar, prelado trasladado desde Sigüenza y cuyo pontificado se extiende entre los años 1348 y 1351, momento en que es promovido a Toledo. Se trata de una figura que permanecía hasta ahora en la completa penumbra de la historia de la iglesia compostelana, hasta el punto de haber desaparecido en muchos casos de las consideraciones históricas, que obviaban o simplemente desconocían su figura, extendiendo las prelaturas limítrofes, las de Pedro V y Gómez Manrique, hasta eliminar la suya. Ello responde a la completa falta de fuentes y menciones existente en los archivos compostelanos. Para paliar este hecho, recurrimos tanto a la bibliografía de otras sedes como, especialmente, a las fuentes del Archivo Secreto Vaticano, con cuya ayuda podemos configurar una primera, novedosa y relativamente completa visión de su gobierno en Santiago.

  4. Dissolved organic nitrogen (DON) losses from nested artificially drained lowland catchments with contrasting soil types

    Science.gov (United States)

    Tiemeyer, Bärbel; Kahle, Petra; Lennartz, Bernd

    2010-05-01

    Artificial drainage is a common practice to improve moisture and aeration conditions of agricultural land. It shortens the residence time of water in the soil and may therefore contribute to the degradation of peatlands as well as to the still elevated level of diffuse pollution of surface water bodies, particularly if flow anomalies like preferential flow cause a further acceleration of water and solute fluxes. Especially in the case of nitrate, artificially drained sub-catchments are found to control the catchment-scale nitrate losses. However, it is frequently found that nitrate losses and nitrogen field balances do not match. At the same time, organic fertilizers are commonly applied and, especially in lowland catchments, organic soils have been drained for agricultural use. Thus, the question arises whether dissolved organic nitrogen (DON) forms an important component of the nitrogen losses from artificially drained catchments. However, in contrast to nitrate and even to dissolved organic carbon (DOC), this component is frequently overlooked, especially in nested catchment studies with different soil types and variable land use. Here, we will present data from a hierarchical water quantity and quality measurement programme in the federal state Mecklenburg-Vorpommern (North-Eastern Germany). The monitoring programme in the pleistocene lowland catchment comprises automatic sampling stations at a collector drain outlet (4.2 ha catchment), at a ditch draining arable land on mineral soils (179 ha), at a ditch mainly draining grassland on organic soils (85 ha) and at a brook with a small rural catchment (15.5 km²) of mixed land use and soil types. At all sampling stations, daily to weekly composite samples were taken, while the discharge and the meteorological data were recorded continuously. Water samples were analyzed for nitrate-nitrogen, ammonium-nitrogen and total nitrogen. We will compare two years: 2006/07 was a very wet year (P = 934 mm) with a high summer

  5. Stokes IQUV magnetic Doppler imaging of Ap stars - I. ESPaDOnS and NARVAL observations

    Science.gov (United States)

    Silvester, J.; Wade, G. A.; Kochukhov, O.; Bagnulo, S.; Folsom, C. P.; Hanes, D.

    2012-10-01

    In this paper we describe and evaluate new spectral line polarization observations obtained with the goal of mapping the surfaces of magnetic Ap stars in great detail. One hundred complete or partial Stokes IQUV sequences, corresponding to 297 individual polarized spectra, have been obtained for seven bright Ap stars using the Échelle SpectroPolarimetric Device for the Observation of Stars (ESPaDOnS) and NARVAL high-resolution spectropolarimeters. The targets span a range of masses from approximately 1.8 to 3.4 M⊙, a range of rotation periods from 2.56 to 6.80 d and a range of maximum longitudinal magnetic field strengths from 0.3 to over 4 kG. For three of the seven stars, we have obtained dense phase coverage sampling the entire rotational cycle. These data sets are suitable for immediate magnetic and chemical abundance surface mapping using magnetic Doppler imaging. For the remaining four stars, partial phase coverage has been obtained, and additional observations will be required in order to map the surfaces of these stars. The median signal-to-noise ratio of the reduced observations is over 700 per 1.8 km s-1 pixel. Spectra of all stars show Stokes V Zeeman signatures in essentially all individual lines, and most stars show clear Stokes QU signatures in many individual spectral lines. The observations provide a vastly improved data set compared to previous generations of observations in terms of signal-to-noise ratio, resolving power and measurement uncertainties. Measurement of the longitudinal magnetic field demonstrates that the data are internally consistent within computed uncertainties typically at the 50-100σ level. Data are also shown to be in excellent agreement with published observations and in qualitative agreement with the predictions of published surface structure models. In addition to providing the foundation for the next generation of surface maps of Ap stars, this study establishes the performance and stability of the ESPaDOnS and NARVAL

  6. Don Delillo’s Point Omega; Ecstasy and Inertia in a Hyperreal World: A Baudrillardian Reading

    Directory of Open Access Journals (Sweden)

    Faeze Yegane

    2015-07-01

    Full Text Available This paper aims to present a Baudrillardian reading of Don Delillo’s Point Omega in the framework of Baudrillard’s definition of the contemporary world as ‘hyperreal’ and also his twin concepts of ‘ecstasy and inertia’. According to Baudrillard, the contemporary time is the hyperreal era in which subjects do not have access to ‘real’ primarily because they are supplied with the ‘simulations’ first and then with the ‘real’ entity and probably never confronted with the ‘real’ itself through media, advertisements, and virtual world. Thus, the perception they have from incidents, objects, places and even other people is ‘hyperreal’; edited, censored, beautified and exaggerated versions of reality; more real than real. In this study Point Omega will be examined as Delillo’s ‘hyperreal’ version of Alfred Hitchcock’s Psycho since the movie is screened in the course of the novel and despite the similarities between the novel and the movie they end contrastingly. Symbolically ‘real’ is not found in the novel due to ‘Mobius spiraling negativity’ which is one of the features of Baudrillard’s definition of ‘hyperreal’ age. Baudrillard believes in the triumph of objects over subjects. While the object’s world is perpetually cultivating frenziedly, objects and technologies begin to dominate the stupefied subjects consequently he states when the objects are moving toward their ‘ecstasy’, the subjects are stricken in ‘inertia’. This supremacy of objects and technologies will be displayed in Point Omega regarding Richard Elster’s inert behavior and reaching the ‘omega point’ that Teilhard de Chardin envisions for human race is rendered impossible due to Elster’s destiny in the framework of Baudrillard’s concept of evolution.  Keywords: Ecstasy, Inertia, Hyperreality, Baudrillard, Don Delillo, Point Omega, Omega Point, Teilhard de Chardin, Alfred Hitchcock.    

  7. El Don Juan Tenorio de Zorrila: entre el carnival y la cuaresma*

    Directory of Open Access Journals (Sweden)

    Ana Alcolea

    1999-12-01

    Full Text Available Mucha tinta ha cobrado forma de escritura en torno a la figura de Don Juan. Y esto, no sólo en estudios bibliográficos y aparato critico, sino y sobre todo, en la casi infinidad de versiones liricas, novelísticas, y dramáticas que a lo largo de los cuatro últimos siglos han tenido como protagonista al "notorio" sevillano o a cualquiera de sus hijos literarios. La obra del vallisoletano José Zorrilla ha sido, sin duda, la que en España se ha convertido en la referencia más tradicional del asunto donjuanesco. En estas páginas vamos a intentar acercarnos a ella a tráves de sus lazos con los mundos del carnaval y Ia cuaresma que, a mi modo de ver, generan y articulan las dos partes que estructuran externa e internamente el drama. Para ello, primero pasearemos brevemente por los orígenes miticos del donjuanismo, que nos llevarán a entornos escatológicos que fusionan mitos y leyendas de diversos linajes: bíblico, griego, hindú, germánico...

  8. What consumers don't know about genetically modified food, and how that affects beliefs.

    Science.gov (United States)

    McFadden, Brandon R; Lusk, Jayson L

    2016-09-01

    In the debates surrounding biotechnology and genetically modified (GM) food, data from consumer polls are often presented as evidence for precaution and labeling. But how much do consumers actually know about the issue? New data collected from a nationwide U.S. survey reveal low levels of knowledge and numerous misperceptions about GM food. Nearly equal numbers of consumers prefer mandatory labeling of foods containing DNA as do those preferring mandatory labeling of GM foods. When given the option, the majority of consumers prefer that decisions about GM food be taken out of their hands and be made by experts. After answering a list of questions testing objective knowledge of GM food, subjective, self-reported knowledge declines somewhat, and beliefs about GM food safety increase slightly. Results suggest that consumers think they know more than they actually do about GM food, and queries about GM facts cause respondents to reassess how much they know. The findings question the usefulness of results from opinion polls as a motivation for creating public policy surrounding GM food.-McFadden, B. R., Lusk, J. L. What consumers don't know about genetically modified food, and how that affects beliefs.

  9. We don’t need another hero!

    Directory of Open Access Journals (Sweden)

    Carlos Renato Zacharias

    2010-12-01

    Full Text Available "We Don't Need Another Hero” was the hit theme song performed by Tina Turner in Mad Max Beyond Thunderdome, a 1985 sci-fi movie. The story was about a post-apocalyptical world representing the timeless and mythical struggle between Good and Evil and the hope for a better future. Children waited for a messiah, who would lead them back to a peaceful civilization! But the world changed, there was no guide and not even the intended place existed any longer! ... Maybe we do need a new hero. But not to lead us back to the world of matter, but to show us new paths to thread. Such paths might already exist, but if they do, they are hidden to us. Signs are vain when one is blind and closed to new opportunities and possibilities. Perhaps this is one further barrier to acknowledge and overcome in order to be ready and able to recognize and interpret new signs. Maybe we, ourselves, are our own awaited hero!

  10. E-mail netiquette for the medical practice employee: 50 do's and don'ts.

    Science.gov (United States)

    Hills, Laura

    2011-01-01

    E-mail has at one and the same time become a primary communication tool for many medical practice employees and a source of untold frustration, misunderstandings, and stress. This article provides a total of 50 rules (25 do's and 25 don'ts) for e-mail netiquette to help medical practice employees use e-mail more effectively. It offers suggestions about the optimal format of e-mails, tips for writing concise e-mail texts, and guidance about the most pressing e-mail privacy and confidentiality concerns. Medical practice employees can use the practical tips presented in this article to help them save time and use their medical practices' e-mail system more productively and effectively. This article also offers suggestions for creating and using a medical practice e-mail disclaimer statement. It describes the six legal threats a disclaimer statement can prevent and the typical content for such disclaimers. Finally, this article includes guidance about creating and implementing a medical practice e-mail policy and provides an outline of typical e-mail policy content. PMID:22111286

  11. Stokes IQUV Magnetic Doppler Imaging of Ap stars I. ESPaDOnS and NARVAL Observations

    CERN Document Server

    Silvester, J; Kochukhov, O; Bagnulo, S; Folsom, C P; Hanes, D

    2012-01-01

    In this paper we describe and evaluate new spectral line polarisation observations obtained with the goal of mapping the surfaces of magnetic Ap stars in great detail. One hundred complete or partial Stokes IQUV sequences, corresponding to 297 individual polarised spectra, have been obtained for 7 bright Ap stars using the ESPaDOnS and NARVAL spectropolarimeters. The targets span a range of mass from approximately 1.8 to 3.4 solar mass, a range of rotation period from 2.56 to 6.80 days, and a range of maximum longitudinal magnetic field strength from 0.3 to over 4 kG. For 3 of the 7 stars, we have obtained dense phase coverage sampling the entire rotational cycle. These datasets are suitable for immediate magnetic and chemical abundance surface mapping using Magnetic Doppler Imaging (MDI). For the 4 remaining stars, partial phase coverage has been obtained, and additional observations will be required in order to map the surfaces of these stars. The median signal-to-noise ratio of the reduced observations is ...

  12. Inversion of stellar fundamental parameters from ESPaDOnS and Narval high-resolution spectra

    Science.gov (United States)

    Paletou, F.; Böhm, T.; Watson, V.; Trouilhet, J.-F.

    2015-01-01

    The general context of this study is the inversion of stellar fundamental parameters from high-resolution Echelle spectra. We aim at developing a fast and reliable tool for the post-processing of spectra produced by ESPaDOnS and Narval spectropolarimeters. Our inversion tool relies on principal component analysis. It allows reducing dimensionality and defining a specific metric for the search of nearest neighbours between an observed spectrum and a set of observed spectra taken from the Elodie stellar library. Effective temperature, surface gravity, total metallicity, and projected rotational velocity are derived. Various tests presented in this study that were based solely on information coming from a spectral band centred on the Mg i b-triplet and had spectra from FGK stars are very promising. Based on observations obtained at the Télescope Bernard Lyot (TBL, Pic du Midi, France), which is operated by the Observatoire Midi-Pyrénées, Université de Toulouse, Centre National de la Recherche Scientifique (France) and the Canada-France-Hawaii Telescope (CFHT) which is operated by the National Research Council of Canada, CNRS/INSU and the University of Hawaii (USA).

  13. Shattering Man’s Fundamental Assumptions in Don DeLillo’s Falling Man

    Directory of Open Access Journals (Sweden)

    Hazim Adnan Hashim

    2016-09-01

    Full Text Available The present study addresses effects of traumatic events such as the September 11 attacks on victims’ fundamental assumptions. These beliefs or assumptions provide individuals with expectations about the world and their sense of self-worth. Thus, they ground people’s sense of security, stability, and orientation. The September 11 terrorist attacks in the U.S.A. were very tragic for Americans because this fundamentally changed their understandings about many aspects in life. The attacks led many individuals to build new kind of beliefs and assumptions about themselves and the world. Many writers have written about the human ordeals that followed this incident. Don DeLillo’s Falling Man reflects the traumatic repercussions of this disaster on Americans’ fundamental assumptions. The objective of this study is to examine the novel from the traumatic perspective that has afflicted the victims’ fundamental understandings of the world and the self. Individuals’ fundamental understandings could be changed or modified due to exposure to certain types of events like war, terrorism, political violence or even the sense of alienation. The Assumptive World theory of Ronnie Janoff-Bulman will be used as a framework to study the traumatic experience of the characters in Falling Man. The significance of the study lies in providing a new perception to the field of trauma that can help trauma victims to adopt alternative assumptions or reshape their previous ones to heal from traumatic effects.Keywords: benevolence, meaningfulness, self-worthiness, trauma, post-traumatic stress disorder

  14. "Don't know" answers concerning somatic disease status should not be regarded as "no" responses

    Directory of Open Access Journals (Sweden)

    Baumeister, Harald

    2008-06-01

    Full Text Available Background: With regard to patients' self-reported somatic diseases some researchers transformed “don't know (DK” responses into “no” responses. The present study examines the appropriateness of this procedure. Methods: Analyses were based on the nationally representative German National Health Interview and Examination Survey (GHS, which assessed both self-reported diseases and physician-diagnosed diseases (N = 7124. Prevalence rates of persons’ DK responses and the corresponding prevalences of physicians’ diagnoses were calculated for persons with hypertension, coronary heart disease (CHD, heart failure, asthma, chronic bronchitis, thyroid disease, diabetes, cancer, gout, arthrosis, arthritis and osteoporosis. Correlates of physicians' diagnosed diseases of DK cases are reported. Results: Between 1.6% and 9.8% of the participants responded with DK to the question of whether they have the disease. In 3.7% to 29.5% of DK cases, the physicians did regard the respective disease as being present. With regard to persons who responded with DK, the probability of a physicians' diagnosis was increased in the case of increased age and a higher number of somatic comorbidities. Conclusion: The procedure of transforming DK responses into “no” answers does not appear to be recommendable.

  15. Analysis of Multiple Partially Ordered Responses to Belief Items with Don't Know Option.

    Science.gov (United States)

    Ip, Edward H; Chen, Shyh-Huei; Quandt, Sara A

    2016-06-01

    Understanding beliefs, values, and preferences of patients is a tenet of contemporary health sciences. This application was motivated by the analysis of multiple partially ordered set (poset) responses from an inventory on layman beliefs about diabetes. The partially ordered set arises because of two features in the data-first, the response options contain a Don't Know (DK) option, and second, there were two consecutive occasions of measurement. As predicted by the common sense model of illness, beliefs about diabetes were not necessarily stable across the two measurement occasions. Instead of analyzing the two occasions separately, we studied the joint responses across the occasions as a poset response. Few analytic methods exist for data structures other than ordered or nominal categories. Poset responses are routinely collapsed and then analyzed as either rank ordered or nominal data, leading to the loss of nuanced information that might be present within poset categories. In this paper we developed a general class of item response models for analyzing the poset data collected from the Common Sense Model of Diabetes Inventory. The inferential object of interest is the latent trait that indicates congruence of belief with the biomedical model. To apply an item response model to the poset diabetes inventory, we proved that a simple coding algorithm circumvents the requirement of writing new codes such that standard IRT software could be directly used for the purpose of item estimation and individual scoring. Simulation experiments were used to examine parameter recovery for the proposed poset model. PMID:25479822

  16. El infante don Gabriel y el impresor Ibarra en la obra cumbre del Salustio

    Directory of Open Access Journals (Sweden)

    Olaechea Labayen, Juan B.

    1997-04-01

    Full Text Available Not available.En la obra conocida como el «Salustio de Ibarra», considerada por especialistas domésticos y foráneos como la mejor impresa en España, convergen circunstancias relevantes debidas a la participación intelectual del infante don Gabriel de Borbón y la eficiencia técnica de Joaquín Ibarra. El autor reivindica la autenticidad de los textos atribuidos al infante contra cierto escepticismo sembrado en razón de la elevada alcurnia de la atribución. Seguidamente, se realiza el análisis de las cualidades técnicas de la impresión, eminentes en una época de esplendor del arte impresorio, y en cuya confección colaboraron los más descollantes artistas del buril de aquel período considerado de triunfo de la estampa ilustrada.

  17. The potential for detecting `life as we don't know it' by fractal complexity analysis

    Science.gov (United States)

    Azua-Bustos, Armando; Vega-Martínez, Cristian

    2013-10-01

    Finding life in the Universe entirely different to the one evolved on Earth is probable. This is a significant constraint for life-detecting instruments that were sent and may be sent elsewhere in the solar system, as how could we detect life as `we don't know it'? How could we detect something when we have no prior knowledge of its composition or how it looks like? Here we argue that disregarding the type of lifeform that could be envisioned, all must share in common the attribute of being entities that decrease their internal entropy at the expense of free energy obtained from its surroundings. As entropy quantifies the degree of disorder in a system, any envisioned lifeform must have a higher degree of order than its supporting environment. Here, we show that by using fractal mathematics analysis alone, one can readily quantify the degree of entropy difference (and thus, their structural complexity) of living processes (lichen growths and plant growing patterns in this case) as distinct entities separate from its similar abiotic surroundings. This approach may allow possible detection of unknown forms of life based on nothing more than entropy differentials of complementary datasets. Future explorations in the solar system, like Mars or Titan, may incorporate this concept in their mission planning in order to detect potential endemic lifeforms.

  18. Analysis of Multiple Partially Ordered Responses to Belief Items with Don't Know Option.

    Science.gov (United States)

    Ip, Edward H; Chen, Shyh-Huei; Quandt, Sara A

    2016-06-01

    Understanding beliefs, values, and preferences of patients is a tenet of contemporary health sciences. This application was motivated by the analysis of multiple partially ordered set (poset) responses from an inventory on layman beliefs about diabetes. The partially ordered set arises because of two features in the data-first, the response options contain a Don't Know (DK) option, and second, there were two consecutive occasions of measurement. As predicted by the common sense model of illness, beliefs about diabetes were not necessarily stable across the two measurement occasions. Instead of analyzing the two occasions separately, we studied the joint responses across the occasions as a poset response. Few analytic methods exist for data structures other than ordered or nominal categories. Poset responses are routinely collapsed and then analyzed as either rank ordered or nominal data, leading to the loss of nuanced information that might be present within poset categories. In this paper we developed a general class of item response models for analyzing the poset data collected from the Common Sense Model of Diabetes Inventory. The inferential object of interest is the latent trait that indicates congruence of belief with the biomedical model. To apply an item response model to the poset diabetes inventory, we proved that a simple coding algorithm circumvents the requirement of writing new codes such that standard IRT software could be directly used for the purpose of item estimation and individual scoring. Simulation experiments were used to examine parameter recovery for the proposed poset model.

  19. Synthesis of silver nanoparticles using leaves of Catharanthus roseus Linn. G. Don and their antiplasmodial activities

    Institute of Scientific and Technical Information of China (English)

    Ponarulselvam S; Panneerselvam C; Murugan K; Aarthi N; Kalimuthu K; Thangamani S

    2012-01-01

    Objective: To develop a novel approach for the green synthesis of silver nanoparticles using aqueous leaves extracts of Catharanthus roseus (C. roseus) Linn. G. Don which has been proven active against malaria parasite Plasmodium falciparum (P. falciparum). Methods:Characterizations were determined by using ultraviolet-visible (UV-Vis) spectrophotometry, scanning electron microscopy (SEM), energy dispersive X-ray and X-ray diffraction. Results:SEM showed the formation of silver nanoparticles with an average size of 35-55 nm. X-ray diffraction analysis showed that the particles were crystalline in nature with face centred cubic structure of the bulk silver with the broad peaks at 32.4, 46.4 and 28.0. Conclusions: It can be concluded that the leaves of C. roseus can be good source for synthesis of silver nanoparticle which shows antiplasmodial activity against P. falciparum. The important outcome of the study will be the development of value added products from medicinal plants C. roseus for biomedical and nanotechnology based industries.

  20. 60 years of CERN at UNESCO | Don't miss the webcast! | 1 July

    CERN Multimedia

    2014-01-01

    Don't miss the live webcast of the commemoration ceremony, marking the signature of the CERN Convention. The ceremony will be held at the UNESCO Headquarters in Paris on 1 July, from 10 a.m.   To watch the webcast, please visit: http://webcast.web.cern.ch/webcast/ from 10 a.m. Programme 10 a.m. - 11 a.m.: Opening of Ceremony by Maciej Nalecz, Director of the Division of Science Policy and Capacity Building, UNESCO   Address by Irina Bokova, Director-General of UNESCO   Address by Rolf Heuer, Director-General of CERN   Video clip “CERN: 60 years of science for peace”   Address by Agnieszka Zalewska, President of the CERN Council   Hommage à François de Rose, un des pères fondateurs du CERN, par sa fille Laurence Rousselot   CERN as seen by a young phycisist, Claire Lee (ATLAS Collaboration) 11 a.m. - 12 p.m.: Roundtable discussion on “Science for...

  1. E-mail netiquette for the medical practice employee: 50 do's and don'ts.

    Science.gov (United States)

    Hills, Laura

    2011-01-01

    E-mail has at one and the same time become a primary communication tool for many medical practice employees and a source of untold frustration, misunderstandings, and stress. This article provides a total of 50 rules (25 do's and 25 don'ts) for e-mail netiquette to help medical practice employees use e-mail more effectively. It offers suggestions about the optimal format of e-mails, tips for writing concise e-mail texts, and guidance about the most pressing e-mail privacy and confidentiality concerns. Medical practice employees can use the practical tips presented in this article to help them save time and use their medical practices' e-mail system more productively and effectively. This article also offers suggestions for creating and using a medical practice e-mail disclaimer statement. It describes the six legal threats a disclaimer statement can prevent and the typical content for such disclaimers. Finally, this article includes guidance about creating and implementing a medical practice e-mail policy and provides an outline of typical e-mail policy content.

  2. Don Draper Thinks Your Ad Is Cliché: Fairy Tale Iconography in TV Commercials

    Directory of Open Access Journals (Sweden)

    Preston Wittwer

    2016-05-01

    Full Text Available When examining the history of fairy tale iconography in advertising, folklore scholar Donald Haase’s fairy tale encyclopedia compared the Pied Piper of Hamelin to a symbol of advertising who could “play his pipe ever so sweetly and the consumers following him without resisting his charming and manipulative music.” In contrast, a 2012 episode of Mad Men, advertising luminary Don Draper shoots down a shoe commercial pitch featuring Cinderella, calling the idea “cliché”. The temptation for advertisers to rely on fairy tale figures and iconography continues today and many ignore Don’s aversion for cliché because it still gets the job done. However, there are some ads featuring fairy tales which avoid cliché and are truly innovative for their time. I’ll examine how, and for whom, these fairy tale figures have been adapted decade by decade in order to examine popular culture’s commercialized and hypnotic relationship with fairy tales in the most direct format available: television commercials.

  3. Effect of Salinity Stress on Seed Germination Catharanthus roseus Don. Cvs. Rosea and Alba

    Directory of Open Access Journals (Sweden)

    Zahra Rezaee

    2012-03-01

    Full Text Available This experiment was carried out aiming to determine the Catharanthus roseus Don resistance against salinity, due to the increasing salinity of soil, and the importance of this plant as an ornamental flower, as well as the little information available on its tolerance against salinity during the germination period. In order to an experiment was conducted in randomized completely design factorial. Sodium chloride was used for induce salinity stress. These factors include cultivar and salinity levels with Electrical Conductivity (EC of 1, 2, 4, 6, 8, and 10 dS/m. The results of variance analysis showed that salinity effect at level 1% on the germination percentage, germination rate, radicle and plumule length, seedling wet and dry weight and ratio of plumule length to radicle length were significant and they were reduced. While the cultivar and interaction of cultivar and salinity treatments were non significant. According to the results, response of two cultivars to the salinity was the same and there was no difference between them.

  4. Synthesis of silver nanoparticles using leaves of Catharanthus roseus Linn. G. Don and their antiplasmodial activities

    Science.gov (United States)

    Ponarulselvam, S; Panneerselvam, C; Murugan, K; Aarthi, N; Kalimuthu, K; Thangamani, S

    2012-01-01

    Objective To develop a novel approach for the green synthesis of silver nanoparticles using aqueous leaves extracts of Catharanthus roseus (C. roseus) Linn. G. Don which has been proven active against malaria parasite Plasmodium falciparum (P. falciparum). Methods Characterizations were determined by using ultraviolet-visible (UV-Vis) spectrophotometry, scanning electron microscopy (SEM), energy dispersive X-ray and X-ray diffraction. Results SEM showed the formation of silver nanoparticles with an average size of 35–55 nm. X-ray diffraction analysis showed that the particles were crystalline in nature with face centred cubic structure of the bulk silver with the broad peaks at 32.4, 46.4 and 28.0. Conclusions It can be concluded that the leaves of C. roseus can be good source for synthesis of silver nanoparticle which shows antiplasmodial activity against P. falciparum. The important outcome of the study will be the development of value added products from medicinal plants C. roseus for biomedical and nanotechnology based industries. PMID:23569974

  5. Proteomic profiling of proteins associated with the rejuvenation of Sequoia sempervirens (D. Don Endl

    Directory of Open Access Journals (Sweden)

    Chen Yu-Ting

    2010-12-01

    Full Text Available Abstract Background Restoration of rooting competence is important for rejuvenation in Sequoia sempervirens (D. Don Endl and is achieved by repeatedly grafting Sequoia shoots after 16 and 30 years of cultivation in vitro. Results Mass spectrometry-based proteomic analysis revealed three proteins that differentially accumulated in different rejuvenation stages, including oxygen-evolving enhancer protein 2 (OEE2, glycine-rich RNA-binding protein (RNP, and a thaumatin-like protein. OEE2 was found to be phosphorylated and a phosphopeptide (YEDNFDGNSNVSVMVpTPpTDK was identified. Specifically, the protein levels of OEE2 increased as a result of grafting and displayed a higher abundance in plants during the juvenile and rejuvenated stages. Additionally, SsOEE2 displayed the highest expression levels in Sequoia shoots during the juvenile stage and less expression during the adult stage. The expression levels also steadily increased during grafting. Conclusion Our results indicate a positive correlation between the gene and protein expression patterns of SsOEE2 and the rejuvenation process, suggesting that this gene is involved in the rejuvenation of Sequoia sempervirens.

  6. Monoamine oxidase B and free radical scavenging activities of natural flavonoids in Melastoma candidum D. Don.

    Science.gov (United States)

    Lee, M H; Lin, R D; Shen, L Y; Yang, L L; Yen, K Y; Hou, W C

    2001-11-01

    Monoamine oxidase type B (MAO-B) activity and free radicals are elevated in certain neurological diseases. Four natural flavonoids, quercitrin, isoquercitrin, rutin, and quercetin, were isolated for the first time from the leaves of Melastoma candidum D. Don. They exhibited an inhibitory effect on MAO-B. These potent flavonoids were purified using bioassay-guided fractionation and were separated by Diaion, Sephadex LH-20, and MCI CHP20P columns. The IC(50) values of the four potent flavonoids, quercitrin, isoquercitrin, rutin, and quercetin on monoamine oxidase were 19.06, 11.64, 3.89, and 10.89 microM and enzyme kinetics analysis revealed apparent inhibition constants (K(i)) of 21.01, 2.72, 1.83, and 7.95 microM, respectively, on the substrate, benzylamine. The four potent compounds also exhibited hydroxyl radical scavenging activity as determined using a spin trapping electron spin resonance method. This suggests that the four flavonoids from M. candidum possess both MAO-B inhibitory and free radical scavenging activities. These important properties may be used for preventing some neurodegenerative diseases in the future. PMID:11714358

  7. CELL-SURFACE BINDING OF DEOXYNIVALENOL TO Lactobacillus paracasei subsp. tolerans ISOLATED FROM SOURDOUGH STARTER CULTURE

    Directory of Open Access Journals (Sweden)

    Yousef I. Hassan

    2013-04-01

    Full Text Available Deoxynivalenol (DON and fumonisin B1 (FB1 are two contaminant-mycotoxins frequently found in food commodities produced under poor conditions. Several methods have been suggested for the detoxification of such mycotoxins. Among the proposed methods, biological detoxification seems to be the most promising and cost-efficient. This study explores the capability of one strain of lactic acid bacteria, identified as Lactobacillus paracasei subsp. tolerans, to bind both DON and FB1 in liquid cultures. Here we report the ability of heat-inactivated cells to significantly reduce concentrations of DON in liquid cultures. Further mechanistic investigation showed that the detoxification process is a result of the physical binding of such mycotoxins to the cell wall of this bacterium.

  8. Immunomodulatory effects of individual and combined mycotoxins in the THP-1 cell line.

    Science.gov (United States)

    Solhaug, A; Karlsøen, L M; Holme, J A; Kristoffersen, A B; Eriksen, G S

    2016-10-01

    Mycotoxins commonly contaminate food and may pose a risk for disease in humans and animals. As they frequently co-occur, mixed exposures often take place. Monocyte function, including differentiation into active macrophages, is a central part of the immune response. Here we studied effects of naturally co-occurring mycotoxins in grain on monocyte function, and effects of individual and combined exposure on the differentiation process from monocytes into macrophages. The THP-1 cell line was used as a model system. The mycotoxins 2-amino-14,16-dimethyloctadecan-3-ol (AOD), alternariol (AOH), enniatin B (ENNB), deoxynivalenol (DON), sterigmatocystin (ST) and zearalenone (ZEA) differently affected cell viability in THP-1 monocytes, with DON as the most potent. AOH, ZEA and DON inhibited differentiation from monocytes into macrophages. Using this differentiation model, combined exposure of AOH, ZEA and DON were mainly found to be additive. However, the combination AOH+ZEA had somewhat synergistic effect at lower concentrations. Furthermore, alterations in macrophage functionality were found, as single exposure of AOH and ZEA inhibited lipopolysaccharide (LPS) induced TNF-α secretion, while DON increased this response. Overall, the mycotoxins affected monocyte viability and differentiation into macrophages differently. Combined exposures affected the differentiation process mainly additively. PMID:27453131

  9. Computational methods for multi-omic models of cell metabolism and their importance for theoretical computer science

    OpenAIRE

    Angione, Claudio

    2015-01-01

    To paraphrase Stan Ulam, a Polish mathematician who became a leading figure in the Manhattan Project, in this dissertation I focus not only on how computer science can help biologists, but also on how biology can inspire computer scientists. On one hand, computer science provides powerful abstraction tools for metabolic networks. Cell metabolism is the set of chemical reactions taking place in a cell, with the aim of maintaining the living state of the cell. Due to the intrinsic complexity of...

  10. Genetics of asthma: a molecular biologist perspective

    Directory of Open Access Journals (Sweden)

    Ghosh Balaram

    2009-05-01

    Full Text Available Abstract Asthma belongs to the category of classical allergic diseases which generally arise due to IgE mediated hypersensitivity to environmental triggers. Since its prevalence is very high in developed or urbanized societies it is also referred to as "disease of civilizations". Due to its increased prevalence among related individuals, it was understood quite long back that it is a genetic disorder. Well designed epidemiological studies reinforced these views. The advent of modern biological technology saw further refinements in our understanding of genetics of asthma and led to the realization that asthma is not a disorder with simple Mendelian mode of inheritance but a multifactorial disorder of the airways brought about by complex interaction between genetic and environmental factors. Current asthma research has witnessed evidences that are compelling researchers to redefine asthma altogether. Although no consensus exists among workers regarding its definition, it seems obvious that several pathologies, all affecting the airways, have been clubbed into one common category called asthma. Needless to say, genetic studies have led from the front in bringing about these transformations. Genomics, molecular biology, immunology and other interrelated disciplines have unearthed data that has changed the way we think about asthma now. In this review, we center our discussions on genetic basis of asthma; the molecular mechanisms involved in its pathogenesis. Taking cue from the existing data we would briefly ponder over the future directions that should improve our understanding of asthma pathogenesis.

  11. Toward Better Physics Labs for Future Biologists

    CERN Document Server

    Moore, K; Losert, W

    2013-01-01

    We have developed a set of laboratories and hands on activities to accompany a new two-semester interdisciplinary physics course that has been successfully developed and tested in two small test classes of students at the University of Maryland, College Park (UMD) in 2012-2013. We have designed the laboratories to be taken accompanying a reformed course in the student's second year, with calculus, biology, and chemistry as prerequisites. This permits the laboratories to include significant content on physics relevant to cellular scales, from chemical interactions to random motion and charge screening in fluids. We also introduce the students to research-grade equipment and modern physics analysis tools in contexts relevant to biology, while maintaining the pedagogically valuable open-ended laboratory structure of reformed laboratories. Preliminary student results from these two small test classes are discussed.

  12. Hilda Cid: physicist, crystallographer, structural biologist

    Science.gov (United States)

    Gutiérrez, Gonzalo

    2016-05-01

    A brief review of Dr. Hilda Cid, Chilean scientist who excelled in the field of crystallography, is presented. Dr. Cid was born in 1933 in Talcahuano, where she completed her primary and secondary education, graduating as a Teacher of mathematics and physics from the University of Chile in 1958. She continued graduate studies at MIT, USA, where obtained the PhD in 1964. Upon her return to Chile, she joined the Austral University in Valdivia, where she dedicated to characterize, by X-ray, biological material. During that time, she was actively involved in the social changes taking place in the university and in the country. Thus, following the bloody coup of 1973, she and her family were persecuted and forced to go into exile to Sweden. In the decade of the 1980 she returns to Chile, and settles at University of Concepción, where she teaches, conducts research and organizes a number of latino-american courses and symposiums. Knowing that Hilda Cid formed a real school, where her students -now distinguished professionals and researchers- cast their teachings through Chile and other countries, we ended the article with a reflection about why Hilda Cid, despite its obvious scientific achievements, remains ignored by most of the scientific establishment in Chile.

  13. Intelligent systems for the molecular biologist

    Energy Technology Data Exchange (ETDEWEB)

    Brutlag, D.L.

    1995-12-31

    This tutorial was one of eight tutorials selected to be presented at the Third International Conference on Intelligent Systems for Molecular Biology which was held in the United Kingdom from July 16 to 19, 1995. In this paper, one objective is to identify properties of DNA sequences that determine their function, by computer-aided statistical analysis and to accurately predict its function, given a new sequence. A related problem is to predict protein structure and function from the sequence.

  14. DON QUIJOTE COMO NOVELA MODERNA Y LA CONJUNCIÓN DE GÉNEROS ALTOS Y BAJOS Don Quixote as a modern novel and the mixture of high and low genres

    Directory of Open Access Journals (Sweden)

    Manuel Jofré

    2006-07-01

    Full Text Available Partiendo de los aportes de la crítica cervantina, se indagan las nuevas razones para considerar El Quijote como primera novela moderna, a partir de las ideas de Mijail Bajtín. La gran obra de Cervantes emergería no sólo como superación de la línea monológica sino como intersección entre la línea monológica y la línea dialógica de la literatura universal. Esto se manifestaría en la conjunción de géneros altos con géneros bajos, primero, y segundo, en la interfertilización de los discursos sublimes y caballerescos de don Quijote con los discursos campesinos y populares de Sancho. Este verdadero dialogismo se basaría en la intertextualidad de la novela de Cervantes con el diálogo socrático y con la sátira menipea de la antigüedad.Within the context of the Cervantine criticism, new reasons are discussed in this article to consider Don Quixote as the first modern novel, from the point of view of Mijail Bakhtin writings. Cervantes's great work would not only overcome the monologic line, but more precisely it would constitute an intersection between the monologic and dialogic lines of the Universal Literature. This would be evident in the mixture of higher and lower literary genre and in the opposition between Don Quixote´s sublime and knight-like speeches and Sancho´s folk and popular speeches. This truly dialogic relationship would be based upon the intertextuality of Cervantes’s work with the Socratic dialog and the Mineppean satire of antiquity.

  15. La sombra del arcediano. El linaje oculto de don Lorenzo Galíndez de Carvajal

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    Baltasar CUART MONER

    2009-11-01

    Full Text Available RESUMEN: D. Lorenzo Galíndez de Carvajal fue un personaje destacado en la Corte de los Reyes Católicos y Carlos V, además de cronista real. Sin embargo, para alcanzar esta posición tuvo que manipular sus orígenes familiares, siendo como era hijo de un arcediano de Coria y de una moza de servicio en una posada. Las manipulaciones de don Lorenzo tuvieron el éxito apetecido, sin embargo, treinta años después de su muerte, las informaciones realizadas sobre un nieto suyo que quería ingresar en el Colegio Mayor de Oviedo, en Salamanca, sacaron a la luz toda la estrategia familiar que tuvo que poner en marcha su ilustre abuelo para alcanzar la posición a la que llegó.ABSTRACT: D. Lorenzo Galíndez de Carvajal was one of the most influential courtiers at the Catholic King's court. He, too, was one of their chroniclers, but he had to forge his familiar origins in order to hold his job. He was an illegitimate son; his father was an archdeacon in Coria and his mother a maidservant. D. Lorenzo Galíndez de Carvajal succeeded in hiding his origins and his descendants became related to nobility by marriage. Howeyer all his grandfather's impostures and the ways followed by aristocracy to manipulate its origins were discovered when one of his grandsons tried to be admitted to the Oviedo College about the middle of the XVIth century.

  16. Characterization of Alkaloid Uptake by Catharanthus roseus (L.) G. Don Protoplasts 1

    Science.gov (United States)

    McCaskill, David G.; Martin, DeAndra L.; Scott, A. Ian

    1988-01-01

    The accumulation of alkaloids by protoplasts of Catharanthus roseus (L.) G. Don var. Little Bright Eye was studied to determine the specificity of uptake and the role of ion trapping in the storage of alkaloids. Accumulation of the indole alkaloids vindoline, ajmalicine, tabersonine, and vinblastine was found to be biphasic, with an initial burst of uptake followed by a slow, prolonged phase of accumulation. The concentration and pH dependence of the initial burst of uptake for vindoline suggested that uptake occurred by simple diffusion. Uptake of nicotine was monophasic, with a half life of 5.2 minutes. The accumulation ratio (Ci/Ce) for nicotine at steady state and for the initial burst of uptake for vindoline and ajmalicine suggested that accumulation was driven by the pH gradient between the vacuole and the external assay medium. The second, sustained phase of uptake of vindoline was sensitive to inhibition by either 20 millimolar NaN3 or 0.5 millimolar Cu2+. In azide-treated protoplasts, the uptake for vindoline conformed to the kinetics of simple diffusion, with a half life of 4 minutes. The second phase of uptake for ajmalicine, although sensitive to inhibition by Cu2+, was insensitive to inhibition by NaN3. The biphasic uptake of the indole alkaloids was not due to any significant metabolism. It is concluded that accumulation and storage of the indole alkaloids is due only partly to ion trapping of the alkaloids by the low pH of the vacuole lumen. In the case of vindoline, there appears to be a specific energy-requiring uptake that is not seen with nicotine (which is not endogenous to Catharanthus). Accumulation of ajmalicine appears to involve both ion trapping and an azide-insensitive component, which may be due to complexation with organic counterions and phenolics. PMID:16666154

  17. Wound healing properties and kill kinetics of Clerodendron splendens G. Don, a Ghanaian wound healing plant

    Directory of Open Access Journals (Sweden)

    Stephen Y Gbedema

    2010-01-01

    Full Text Available As part of our general objective of investigating indigenous plants used in wound healing in Ghana, we hereby report our findings from some in vitro and in vivo studies related to wound healing activities of Clerodendron splendens G. Don (Verbanaceae. Methanolic extract of the aerial parts of the plant was tested for antimicrobial activity against Gram positive bacteria (Bacillus subtilis, Staphylococcus aureus, Streptococcus faecalis, Micrococcus flavus, as well as resistant strains of Staph. aureus SA1199B, RN4220 and XU212, Gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteous mirabilis, Klebsiella pneumoniae and Candida albicans using the micro-well dilution method. Survivor-time studies of the microorganisms, radical scavenging activity using 2,2′-diphenylpicrylhydrazyl (DPPH and various in vivo wound healing activity studies were also conducted on the extract. The extract exhibited biostatic action against all the test microorganisms with a Minimum Inhibition Concentration (MIC ranging between 64 and 512 μg/ml and a free radical scavenging property with an IC 50 value of 103.2 μg/ml. The results of the in vivo wound healing tests showed that upon application of C. splendens ointment, there was a reduction in the epithelization period from 26.7 days (control to 13.6 days along with a marked decrease in the scar area from 54.2 mm 2 (control to 25.2 mm 2 . Significant increase in the tensile strength and hydroxyproline content were also observed as compared to the control and was comparable to nitrofurazone. The above results appear to justify the traditional use of C. splendens in wound healing and treatment of skin infections in Ghana.

  18. Sombreamento de plantas de Catharanthus roseus (L.) G. Don 'Pacifica White' por malhas coloridas: desenvolvimento vegetativo Shading of 'Pacifica White' Catharanthus roseus (L.) G. Don plants with colored nets: vegetative development

    OpenAIRE

    Anderson Adriano Martins Melo; Amauri Alves de Alvarenga

    2009-01-01

    As malhas coloridas têm sido utilizadas para manipular o desenvolvimento vegetativo, melhorando a utilização da radiação solar por plantas ornamentais. Objetivou-se, neste trabalho estudar o efeito da redução de 50% da radiação fotossinteticamente ativa sobre o crescimento vegetativo de plantas de Catharanthus roseus (L.) G. Don, por meio de malhas azul e vermelha e malha preta, em comparação com plantas crescidas na ausência de sombreamento (pleno sol). As plantas foram obtidas a partir de s...

  19. [Introduction and transformation of the psychiatric term "anancasm". From Gyula (Julius) Donáth via Kurt Schneider to ICD-10].

    Science.gov (United States)

    Steinberg, H

    2014-09-01

    The conceptual history of "anancasm" in psychiatry remains almost unexplored and this article will help to remove this deficit. It was the Budapest-based neuropsychiatrist Gyula Donáth (1849-1944) who first proposed this Greek-rooted term in 1897 as an international term for compulsive symptoms and as an independent mental illness similar to present-day obsessive compulsive disorders (ICD-10). By suggesting this term Donáth wanted to extend the concept of compulsion as proposed by his teacher Carl Westphal to other compulsive phenomena, psychomotor impulses and restrictions including echolalia, coprolalia, echokinesis, echopraxia, contemporary maladie des tics (present day Tourette's syndrome) and even intermittent dipsomania (craving for alcohol), paraphilias, sexual fetishes and homosexuality. In 1923 Kurt Schneider used this term for a subgroup of psychopathic personalities, the so-called insecure anancastic psychopaths. His concept was much different to that suggested by Donáth, with the only thing in common being the compulsory component. Schneider's anancasts suffered from feelings of insecurity and insufficiency and were forced to try to overcompensate by being excessively careful, meticulous and hyper-correct. Based on Schneider's concept anancasm has survived as a name for a subdivision of compulsive personality disorders in ICD-10; however, these rather complex personality defects were not what Donáth had in mind when he first suggested the term anancasm. The paper discusses further discrepancies between Donáth, Schneider and ICD-10.

  20. The impact of wheat production on the occurrence of mycotoxins DON (deoxynivalenol and ZEA (zearalenone on wheat grains (Triticum aestivum L.

    Directory of Open Access Journals (Sweden)

    Lena TAJNŠEK

    2015-12-01

    Full Text Available The current study has been conceived to draw attention to the factors that should be avoided in the production of cereal grains (such as high doses of nitrogen by analysing differences in cereal grain contamination with mycotoxins DON (deoxynivalenol and ZEA (zearalenone, depending on methods of wheat production. We studied the occurrence of DON and ZEA at very different production intensities in the ‘Reska’ and ‘Savinja’ wheat cultivars which were grown in a long-term field experiment (designed in 1992 at Jable near Ljubljana in the years 2006 and 2008. The cultivars ‘Reska’ and ‘Savinja’ have been considered in crop rotation of wheat-maize-oats in 5 methods of production with variants ranging organic up to intensely conventional. The results obtained have shown that adequately chosen agri-technical measures significantly reduced the risk of contamination of wheat flour by the mycotoxins DON and ZEA. Too abundant N (nitrogen fertilization of either inorganic or organic origin is to be avoided. The extensive wheat growing reduces considerably the risk of DON and ZEA occurrence in comparison to the intensive one. Cultivar plays an important role in this process. The comparison of both cultivars has shown that the cultivar ‘Savinja’ was more resistant to the contamination with DON and ZEA than the cultivar ‘Reska’.

  1. Advances in stem cell research

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@In 1998, biologists Thomson and Gearhart successfully derived stem cells from human embryos. One year later, several researchers discovered that adult stem cells still retain the ability to be differentiated into unrelated types of cells. Advances in stem cell research open a promising direction for applied medical science. Moreover, it may also force scientists to reconsider the fundamental theory about how cells grow up. Stem cell research was considered by Science as the top of the ten breakthroughs of science of the year[1]. This paper gives a survey of recent advances in stem cell research. 1 Overview In the 1980s, embryonic stem cell and/or embryonic germ cell line (ES cell line, EG cell line) of multifarious mammalian animals, especially those of non-human pri-mates, had been established. In 1998, Thomson and Shamblott obtained ES, EG cell lines from human blasto-cysts and gonad ridges of early human embryos, respec-tively. Their research brought up an ethical debate about whether human embryos can be used as experimental materials. It was not appeased until 1999 when research-ers discovered that stem cells from adults still retain the ability to become different kinds of tissue cells. For in-stance, brain cells can become blood cells[2], and cells from bone marrow can become cells in liver. Scientists believe, for a long time, that cells can only be developed from early pluripotent embryo cells; the differentiation potential of stem cells from mature tissues is restricted to only one of the cell types of the tissue where stem cells are obtained. Recent stem cell researches, however, sub-verted the traditional view of stem cells. These discoveries made scientists speed ahead with the work on adult stem cells, hoping to discover whether their promise will rival that of ES cells.

  2. Cells on film - the past and future of cinemicroscopy.

    Science.gov (United States)

    Stramer, Brian M; Dunn, Graham A

    2015-01-01

    Movie making is now a ubiquitous experimental tool that biologists use alongside more traditional techniques such as molecular biology and biochemistry. It is no longer just cell biologists, but scientists from many other disciplines, such as immunology and neuroscience, that utilise movies to dissect their processes of interest. When did filming become such a standard laboratory technique? Who developed the use of the movie as an experimental tool? The Wellcome Library has recently restored and digitized a number of original 16-mm films from two pioneering cinemicroscopists, Ronald Canti and Michael Abercrombie, which are now freely available to the scientific community. In light of these films, this Essay will give a brief history of the early cinemicroscopists and discuss what is driving the use of movies in the laboratory today.

  3. Rhizonema sequoiae n.gen. n.sp. from Coast Redwood Sequoia sempervirens (D.Don) Endl.

    Science.gov (United States)

    Del Prado Vera, I C; Lownsbery, B F; Maggenti, A R

    1983-07-01

    Rhizonema sequoiae n.gen, u. sp. is described from the roots of Coast Redwood, Sequoia sempervirens (D. Don) Endl., growing near Lake Lagunitas, Marin County, California. Rhizonema females are annulated over their entire bodies, are wholly embedded in host tissue, and secrete an abundant amount of gel material. Mature females do not form a cyst. The vulva is located on a large posterior terminal cone, and the anus is on the dorsal vulval lip. Esophageal glands of the second-stage larvae fill more than half of the body cavity. Tails of the vermiform males are blunt, and a cloacal tubus is present.

  4. Stokes IQUV mapping of $\\alpha^2$ CVn & other Ap stars using ESPaDOnS and NARVAL

    OpenAIRE

    Silvester, J.; Kochukhov, O.; Wade, G. A.

    2013-01-01

    New spectral line polarisation observations of 7 bright Ap stars have been obtained with the ESPaDOnS and Narval high resolution spectropolarimeters (Silvester et al. 2012). The aim of this data set is produce a series of surface magnetic field and surface chemistry maps for these Ap stars. We present new magnetic maps for the Ap star $\\alpha^2$ CVn using these new data and the MDI inversion code INVERS10. $\\alpha^2$ CVn is the first Ap star to be observed during two separate epochs using hig...

  5. Relación interartística en los Cuadernos de Don Rigoberto de Mario Vargas Llosa

    OpenAIRE

    León Restrepo, Ana María

    2012-01-01

    A partir de la lectura de la novela del escritor peruano Mario Vargas Llosa Los Cuadernos de Don Rigoberto, el presente artículo pretende hacer una breve descripción de la relación entre arte y literatura, en específico la intertextualidad pintura y producción verbal, presente en dicha obra -- Este artículo se divide en tres apartados: la contextualización de la novela, sus antecedentes y principales motivos de análisis literario, hasta el desarrollo de la relación mencionada en el párrafo an...

  6. Tempo e percezione in The Body Artist di Don DeLillo e Ghost Trio di Samuel Beckett

    Directory of Open Access Journals (Sweden)

    Davide Barbuscia

    2013-03-01

    Full Text Available This essay provides a critical analysis of Don DeLillo’s novel The Body Artist and Samuel Beckett’s work for television Ghost Trio. In particular it analyses the representation of time and its perception in both these works, highlighting some shared aesthetic modalities such as their attempt to depict duration through a poetics of slow motion. The essay also foregrounds how both these works give narrative form to Merleau-Ponty’s understanding of ‘time as its perception’.

  7. Antioxidative Potentials as a Protective Mechanism in Catharanthus roseus (L.) G.Don. Plants under Salinity Stress

    OpenAIRE

    Jaleel, Cheruth Abdul; Gopi, Ragupathi; MANIVANNAN, Paramasivam; Panneerselvam, Rajaram

    2007-01-01

    Antioxidant responses were analysed in Catharanthus roseus (L.) G.Don. under 0, 50 and 100 mM NaCl in order to investigate the plant´s protective mechanisms against long-term salt-induced oxidative stress. The NaCl treatments were repeated in 4 different stages of growth, i.e. 30, 45, 60 and 75 days after sowing (DAS). The plants were uprooted randomly 90 DAS and the non-enzymatic and enzymatic antioxidant potentials were analysed. High salinity caused a decrease in reduced glutathione (GSH) ...

  8. Linkage mapping and identification of QTL affecting deoxynivalenol (DON) content (Fusarium resistance) in oats (Avena sativa L.).

    Science.gov (United States)

    He, Xinyao; Skinnes, Helge; Oliver, Rebekah E; Jackson, Eric W; Bjørnstad, Asmund

    2013-10-01

    Mycotoxins caused by Fusarium spp. is a major concern on food and feed safety in oats, although Fusarium head blight (FHB) is often less apparent than in other small grain cereals. Breeding resistant cultivars is an economic and environment-friendly way to reduce toxin content, either by the identification of resistance QTL or phenotypic evaluation. Both are little explored in oats. A recombinant-inbred line population, Hurdal × Z595-7 (HZ595, with 184 lines), was used for QTL mapping and was phenotyped for 3 years. Spawn inoculation was applied and deoxynivalenol (DON) content, FHB severity, days to heading and maturity (DH and DM), and plant height (PH) were measured. The population was genotyped with DArTs, AFLPs, SSRs and selected SNPs, and a linkage map of 1,132 cM was constructed, covering all 21 oat chromosomes. A QTL for DON on chromosome 17A/7C, tentatively designated as Qdon.umb-17A/7C, was detected in all experiments using composite interval mapping, with phenotypic effects of 12.2–26.6 %. In addition, QTL for DON were also found on chromosomes 5C, 9D, 13A, 14D and unknown_3, while a QTL for FHB was found on 11A. Several of the DON/FHB QTL coincided with those for DH, DM and/or PH. A half-sib population of HZ595, Hurdal × Z615-4 (HZ615, with 91 lines), was phenotyped in 2011 for validation of QTL found in HZ595, and Qdon.umb-17A/7C was again localized with a phenotypic effect of 12.4 %. Three SNPs closely linked to Qdon.umb-17A/7C were identified in both populations, and one each for QTL on 5C, 11A and 13A were identified in HZ595. These SNPs, together with those yet to be identified, could be useful in marker-assisted selection to pyramiding resistance QTL.

  9. Colonel Don Benito Machado. A National Guards commandant on the southern frontier of Buenos Aires [1852-1880] El coronel Don Benito Machado. Un comandante de Guardias Nacionales en la frontera sur bonaerense [1852-1880

    Directory of Open Access Journals (Sweden)

    Leonardo Canciani

    2012-01-01

    Full Text Available In this work, we will analyze the National Guard regiments and the role played by their commandants during the process of national organization on the southern frontier of Buenos Aires, from the case study of coronel don José Benito Machado. First of all, our objective will be describe the specific characteristics that adopted the National Guards in the societies of the southern frontier of the province of Buenos Aires. And then, to analyze the mechanisms developed by Benito Machado to build the power bases in the region that enabled the consolidation of its leadershipEn el presente trabajo, analizamos los regimientos de Guardias Nacionales y las funciones que desempeñaron sus comandantes durante el proceso de organización nacional en la frontera sur bonaerense, desde el estudio de caso del coronel don José Benito Machado. En primer lugar, repasamos las características específicas que adoptaron las Guardias Nacionales en las sociedades de la frontera sur de la provincia de Buenos Aires; mientras que, en segundo lugar, analizamos los diversos mecanismos desarrollados por Benito Machado para construir las bases de poder en la región que posibilitaron el afianzamiento de su liderazgo

  10. Tradução de Don Juan oder Die Liebe zur Geometrie de Max Frisch e a recepção do autor em Portugal

    OpenAIRE

    Corte-Real, Maria Alexanra Viana de Meneses

    2010-01-01

    O projecto de mestrado Tradução de Don Juan oder Die Liebe zur Geometrie de Max Frisch e a Recepção do Autor em Portugal é composto por duas partes distintas. A primeira parte é dedicada a uma pesquisa da recepção portuguesa do autor. Na segunda parte apresenta-se uma proposta de tradução do drama Don Juan oder Die Liebe zur Geometrie, complementada com uma análise relevante para a tradução que engloba exemplos relevantes.

  11. A Glimpse of Stem Cell Research in China

    Institute of Scientific and Technical Information of China (English)

    CHEN Zhi-Guo; ZHANG Y.Alex

    2011-01-01

    2010 has seen rapid progress in stem cell research in China.Not only the major funding agencies had provided extensive funding to stem cell research,the publications by Chinese stem cell biologists also flourished in top-notch scientific journals.In this special review,we highlighted some recent studies that had been published in Science China Life Sciences and Cell Research,two key SCI journals based in China.Not surprisingly,these studies mainly focused on some of the highly pursued stem cell types:embryonic stem cells(ES cells),induced pluripotent stem cells (iPSCs),neural stem cells(NSCs)and mesenchymal stem cells(MSCs).

  12. Comparative Study on the Effect of Different Fertilizers on the Growth and Development of Halenia ellipitica D.Don%不同肥料对椭圆叶花锚生长发育的比较研究

    Institute of Scientific and Technical Information of China (English)

    韩友吉; 李锦萍; 王延花; 张淑玲; 陈桂琛

    2008-01-01

    [Objective] The aim was to study the effect of different treatments on development and growth of Halenia ellipitica D.Don.[Method] Three treatments (urea treatment group, diammonium phosphate treatment group and control group) were set in the experiment with 3 replications to detect plant height,root length, leaf number, branch number, flower number and biomass of Halenia ellipitica D.Don. in all experimental groups.[Result] The leaf number,branch number, flower number and output of crude drug of Halenia ellipitica D.Don. in urea treatment group were more than that of Halenia ellipitica D.Don. in diammonium phosphate treatment group and control group,however,the plant height and root length were not significantly different among all groups.[Conclusion] The urea generated better effect on Halenia ellipitica D.Don. cultivation than that produced by diammonium phosphate treatment.

  13. Tsunami survivors' perspectives on vulnerability and vulnerability reduction: evidence from Koh Phi Phi Don and Khao Lak, Thailand.

    Science.gov (United States)

    Steckley, Marylynn; Doberstein, Brent

    2011-07-01

    This paper presents the results of primary research with 40 survivors of the 2004 Indian Ocean tsunami in two communities: Khao Lak (n=20) and Koh Phi Phi Don (n=20), Thailand. It traces tsunami survivors' perceptions of vulnerability, determines whether residents felt that the tsunami affected different communities differently, identifies the populations and sub-community groups that survivors distinguished as being more vulnerable than others, highlights community-generated ideas about vulnerability reduction, and pinpoints a range of additional vulnerability reduction actions. Tsunami survivors most consistently identified the 'most vulnerable' community sub-populations as women, children, the elderly, foreigners, and the poor. In Khao Lak, however, respondents added 'Burmese migrants' to this list, whereas in Koh Phi Phi Don, they added 'Thai Muslims'. Results suggest that the two case study communities, both small, coastal, tourism-dominated communities no more than 100 kilometres apart, have differing vulnerable sub-groups and environmental vulnerabilities, requiring different post-disaster vulnerability reduction efforts. PMID:21083848

  14. ECOLOGICAL STRESS AND ITS EFFECTS ON ESSENTIAL HYPERTENSION AND CHRONIC OBSTRUCTIVE BRONCHITIS DEVELOPMENT IN ROSTOV-ON-DON INHABITANTS

    Directory of Open Access Journals (Sweden)

    L. I. Katelnitskaya

    2015-12-01

    Full Text Available Aim. To study prevalence of essential hypertension (HT and chronic obstructive bronchitis (COB among Rostov-on-Don population, to assess influence of ecological stress on HT and COB development and to find the ways to improve therapy of combined pathology.Material and methods. Single-stage epidemiologic study of Rostov-on-Don population was carried out. The study included questionnaire to reveal main risk factors of chronic noninfectious diseases, a number of laboratory and instrumental indices.Results. Prevalence of HT and COB is significantly higher in patients, living in ecologically unfavorable districts. Rigidity and stretch ability of vascular wall influence the course and development of HT as well as atrial natriuretic factor. Carbonic anhydrase inhibitor, acetazolamide, when is added to standard therapy increases in its efficacy in blood pressure control, decrease in blood pressure in pulmonary artery and correction of disturbed acidbase balance in patients with HT combined with COB.Conclusion. The connection between unfavorable ecology and HT and COB development is demonstrated. Adjuvant therapy with acetazolamide is proposed to increase in efficacy of HT and COB standard therapy.

  15. Le don après un décès d'origine cardiocirculatoire au Canada

    Science.gov (United States)

    Shemie, Sam D.; Baker, Andrew J.; Knoll, Greg; Wall, William; Rocker, Graeme; Howes, Daniel; Davidson, Janet; Pagliarello, Joe; Chambers-Evans, Jane; Cockfield, Sandra; Farrell, Catherine; Glannon, Walter; Gourlay, William; Grant, David; Langevin, Stéphan; Wheelock, Brian; Young, Kimberly; Dossetor, John

    2006-01-01

    Résumé Ces recommandations sont le fruit d'un processus multidisciplinaire national ayant duré un an et visant à déterminer si et comment l'on pourrait procéder au don d'organes après un décès d'origine cardiocirculatoire («don après le décès cardiocirculatoire», ou DDC) au Canada. Le forum national organisé en février 2005 a permis aux participants de discuter et d'élaborer des recommandations sur les principes, interventions et pratiques se rapportant au DDC. Les aspects éthiques et juridiques ont été abordés dans les discussions. À la fin du Forum, la majorité des participants ont été favorables à l'implantation de programmes de DDC au Canada. Les participants du Forum ont également convenu qu'il fallait formuler et prôner des valeurs fondamentales pour orienter l'élaboration de programmes et de protocoles basés sur le cadre médical, éthique et juridique établi lors de cette réunion. Même si la possibilité d'un don d'organes et de tissus doit faire partie intégrante des soins de fin de vie, il faut insister sur le fait que le devoir de diligence envers les patients mourants et leurs familles doit demeurer la priorité des équipes soignantes. La complexité et les répercussions profondes du décès sont reconnues et doivent être respectées, de même que les différences personnelles, ethnoculturelles et religieuses face à la mort et au don d'organes. Les décisions d'arrêter le traitement de maintien des fonctions vitales, la prise en charge des derniers moments de la vie et le diagnostic de décès selon des critères cardiocirculatoires doivent être distincts et indépendants des processus de don et transplantation. Ce rapport contient des recommandations destinées aux gestionnaires de program, aux autorités sanitaires régionales et aux instances appelés à élaborer les protocoles de DDC. Les programmes doivent être conçus en fonction des éléments suivants : direction et planification locales, éducation et

  16. Effects of aqueous root extract of Carpolobia alba G. Don on sexual behaviour in adult male rats.

    Science.gov (United States)

    Kenmogne, H; Koloko, B; Hambe, C; Domkam, J; Ngaha Njila, M; Bend, E; Oundoum Oundoum, P; Massoma Lembè, D; Dimo, T

    2016-10-01

    Several lines say that a number of natural products, mostly plant based, have been claimed to cure male sexual dysfunction. One of such botanicals is Carpolobia alba G. Don which is used in Cameroon to treat erectile dysfunction and related male sexual debilities. However, the traditional claim has not been scientifically tested. Thirty adult male rats (five groups of six animals) were orally treated daily with distilled water, sildenafil citrate (positive control), 75, 150, 300 mg/kg of C. alba G. Don roots aqueous extract. The erection and ejaculation properties were recorded on the first, seventh and fourteenth day of treatment by following copulation settings: number of erections (NE), mount frequency (MF), intromission frequency (IF), ejaculation frequency (EF), mount latency (ML), intromission latency (IL), ejaculation latency (EL), average interval of copulation (AIC) and post-ejaculatory interval (PEI). The extract resulted in an improvement of copulation parameters through significant reduction (p erectile and pro-ejaculatory potential of aqueous root extract of this plant in male rats. PMID:27681647

  17. Chemical Constituents of Picrasma quassioides (D.Don) Benn.%苦木化学成分研究

    Institute of Scientific and Technical Information of China (English)

    祝晨蔯; 邓贵华; 林朝展

    2012-01-01

    Five compounds were isolated and purified from the 95% ethanol extract of Picrasma quassioides (D. Don) Benn. by using column cinematography and recrystallization. On the basis of spectroscopic methods,their structures were identified as tiifolirhran (1) .maackiain (2) ,3',7-dihydroxy-4'-methoxyisoflavone (3) ,umbelliferone (4) ,and dmodin (5). All the Compounds are reported from the genus Picrasma for the first time.%运用各种柱色谱和重结晶等方法对苦木Pierasma quassioides (D.Don) Benn.进行分离纯化,得到五个化合物.通过NMR等现代波谱学方法和技术鉴定化合物的结构分别为:高丽槐素-3-0-β-D-葡萄糖苷(trifolirhizin,1),高丽槐素(maackiain,2),3',7-二羟基4'-甲氧基异黄酮(3',7 -dihydroxy-4'-methoxyisoflavone,3),7-羟基香豆素(ubelliferone,4)和大黄素(emodin,5).五个化合物均为首次从该属植物中分离得到.

  18. ANALYSIS OF HEAVY METALS CONTENT IN PYLAISIELLA MOSS (PYLAISIA POLYANTHA GROWING IN THE CITY OF ROSTOV-ON-DON

    Directory of Open Access Journals (Sweden)

    Varduni T. V.

    2015-02-01

    Full Text Available The problem of environmental contamination by heavy metals is significant problem of urbanization. The search of ways of indication heavy metals pollution becomes actual, because of their simplicity and affordability. A widely used test objects for heavy metals pollution indicators are leafy mosses used for bioindication, because they are highly sensitive to any stress factor. The research shows that bioindication with using leafy mosses as test objects is highly effective method definition of heavy metals pollution. Using of bioindication methods are promising techniques for the assessment of the contamination of ecosystems by heavy metals. Through the use of this method, it is possible indication of pollution of the surface layer of air with heavy metals. The epiphytic moss (Pylaisia polyantha growing in different zones of the city of Rostov-on-Don, was used for the heavy metals pollution biomonitoring of urbosystems. The accumulation features of heavy metals in the epitaphic pylaisiella moss (Pylaisia polyantha in the territory of the city of Rostov-on-Don have been considered. pylaisiella moss (P. polyantha accumulates the largest amounts of the following heavy metals: Zn, Cr, Pb, Sr, Ni (Kc to 1.07, and Cu. According to the Kc values, all the studied elements accumulated by pylaisiella moss form the following series of biological uptake: Zn > Pb > Sr > Cr > V > Ni > Cu > Cо. The results of investigation showed that the epiphytic moss (Pylaisia polyantha can be used as indicator of heavy metals pollution in different polluted zones

  19. Stem cell research in China: an intertwinement of international finances, ambition, and bioethics

    OpenAIRE

    Sleeboom, Margaret

    2002-01-01

    Stem cell research promises remedies to widespread diseases ranging from diabetes to paralysis. ‘Stem cell’ is a term used to refer to a range of cells that have the ability to divide into specialized body cells, such as blood cells or new tissue. By studying the processes in which stem cells grow and differentiate, biologists study the causes of many diseases, and hope to use them for therapeutic uses in the repair of damaged tissue and organs for a wide range of currently incurable di...

  20. Ground and space based cloud-top wind velocities using CFHT/ESPaDOnS (Doppler velocimetry) and VEx/VIRTIS (cloud tracking) coordinated measurements

    Science.gov (United States)

    Machado, Pedro; Widemann, Thomas; Peralta, Javier; Gonçalves, Rúben; Donati, Jean-François; Luz, David

    2016-04-01

    We will present wind velocity results based in the measurements of the horizontal wind field at the cloud top level of the atmosphere of Venus, near 70 km altitude. Our aim is contribute to the characterisation of the zonal and meridional wind latitudinal profiles on hour and day-timescales. This will be done by tracking Doppler shift of solar and CO2 lines over the dayside hemisphere in coordination with ESA's Venus Express orbiter. Our observations measured winds at cloud tops at latitudes 60°S-60°N, while Vex/VIRTIS privileged southern latitudes poleward of 45°S. This coordination effort intended to provide a combined monitoring of short-term changes of wind amplitude and directions with extensive spatial coverage. We present results based on inter comparison of ground-based Doppler velocimetry of cloud-top winds and cloud tracking measurements from the Venus Express spacecraft. Doppler wind velocimetry obtained with the 3.60 m Canada-France-Hawaii telescope (CFHT) and the Visible Spectrograph ESPaDOnS in April 2014 consisted of high-resolution spectra of Fraunhofer lines in the visible range (0.37-1.05 μm) to measure the wind velocity using the Doppler shift of solar radiation scattered by cloud top particles in the observer's direction. The complete optical spectrum was collected at a phase angle Φ = (76 ± 0.3)°, at a resolution of about 80000. Both ground-based and Venus Express measurements show considerable day-to-day variability revealing wave propagation and angular momentum transport in latitude which needs to be carefully assessed. ESPaDOnS and the sequential technique of visible Doppler velocimetry has proven a reference technique to measure instantaneous winds. These measurements are necessary to help validating Global Circulation Models (GCMs), to extend the temporal coverage of available datasets. The ground-based observations in the base of this project are critical in their complementarity with Venus Express, which was recently

  1. Live cell imaging in Drosophila melanogaster.

    Science.gov (United States)

    Parton, Richard M; Vallés, Ana Maria; Dobbie, Ian M; Davis, Ilan

    2010-04-01

    Although many of the techniques of live cell imaging in Drosophila melanogaster are also used by the greater community of cell biologists working on other model systems, studying living fly tissues presents unique difficulties with regard to keeping the cells alive, introducing fluorescent probes, and imaging through thick, hazy cytoplasm. This article outlines the major tissue types amenable to study by time-lapse cinematography and different methods for keeping the cells alive. It describes various imaging and associated techniques best suited to following changes in the distribution of fluorescently labeled molecules in real time in these tissues. Imaging, in general, is a rapidly developing discipline, and recent advances in imaging technology are able to greatly extend what can be achieved with live cell imaging of Drosophila tissues. As far as possible, this article includes the latest technical developments and discusses likely future developments in imaging methods that could have an impact on research using Drosophila. PMID:20360379

  2. Elemental analysis on the Myanma Tinospora Cordifolia (Hsin-Don-Ma-Nwe) by the energy dispersive X-ray fluorescence technique

    International Nuclear Information System (INIS)

    The application of the energy dispersive X-ray fluorescence technique by using high resolution X-ray detector system has been studied. The Myanma indigenous medicine Tinospora Cordifolia (Hsin-Don-Ma-Nwe) sample was analyzed by using quantitative analysis software (AXIL) provided by IAEA with personal computer

  3. The Vanishing Mexicana/o: (Dis)Locating the Native in Ruiz de Burton's "Who Would Have thought It?" and "The Squatter and the Don"

    Science.gov (United States)

    Szeghi, Tereza M.

    2011-01-01

    This article complements the existing body of Ruiz de Burton scholarship by providing the first sustained examination of her literary representations of American Indians in both "Who Would Have Thought It?" (1872) and "The Squatter and the Don" (1885), and by exploring how these representations serve her broader aims of social and political…

  4. "This Is My Body Which Will Be Given up for You": Federico Garcia Lorca's "Amor de Don Perlimplin" and the "Auto Sacramental" Tradition

    Science.gov (United States)

    Badenes, Jose Ignacio

    2009-01-01

    "Amor de Don Perlimplin con Belisa en su jardin" is one of Federico Garcia Lorca's shortest plays as well as one of his deepest. Its theme is love, particularly the interaction between spiritual longing and carnal desire, body and soul, humanity and divinity. These are aspects found in Roman Catholic Eucharistic theology which the…

  5. Muusikamaailm : Praemium Imperiale kätteandmine. Vanamuusika päevad Hernes. Uus ooper Don Quijotest. Soome muusikud Münchenis / Priit Kuusk

    Index Scriptorium Estoniae

    Kuusk, Priit, 1938-

    1999-01-01

    Jaapani kunstide auhinna "Praemium Imperiale" laureaatidest. Lääne-Saksa Raadio festivalist "Herne", tänavu pealkirja all "Spiel auf, Europa!". C. Halffteri uudisooper "Don Quijote" tuleb maailmaesiettekandele Madridis 2000. a. veebruaris. Soome muusikute kontsertidest sügishooajal Baierimaal ja Münchenis

  6. Low soil water and nutrient availability below New Zealand kauri ( Agathis australis (D. Don) Lindl.) trees increase the relative fitness of kauri seedlings

    NARCIS (Netherlands)

    Verkaik, E.; Berendse, F.; Gardner, R.O.

    2007-01-01

    Tree species can affect the soil they are growing on and this might influence their fitness. The New Zealand gymnosperm tree species kauri (Agathis australis (D. Don) Lindl.) which grows in mixed angiosperm¿gymnosperm forests has a substantial effect upon the soil. We studied the hypotheses that: (1

  7. La fenomenología del don y la dialéctica entre disimetría y mutualidad

    Directory of Open Access Journals (Sweden)

    Roberto J. Walton

    2006-10-01

    Full Text Available RESUMEN: El artículo procura, en primer lugar, esbozar los rasgos fundamentales de la mutualidad del don tal como han sido presentados por P. Ricoeur en Parcours de la reconnaissance. En segundo lugar, se intenta esclarecer el carácter simbólico del don ceremonial recíproco aplicando los rasgos distintivos asignados por Ricoeur al simbolismo inmanente o constructivo. En tercer lugar, se analiza la así llamada economía del don en el terreno del simbolismo representativo con la finalidad de mostrar la función crítica ejercida por el agape sobre la lógica de la equivalencia. Finalmente, se examina el marco general del análisis de Ricoeur sobre la mutualidad a fin de poner de relieve las diferencias entre su interpretación de la disimetría y la version de Lévinas. El trabajo se completa en cada uno de los cuatro pasos con una referencia a otros puntos de vista fenomenológicos que contribuyen a aclarar las tesis de Ricoeur.ABSTRACT: The purpose of this paper is fourfold. First, it undertakes to outline the main features of the mutuality of the gift as developed by P. Ricoeur in Parcours de la reconnaissance. Second, an attempt is made to clarify the symbolic character of the reciprocal ceremonial gift by applying the distinctive traits assigned by Ricoeur to immanent or constructive symbolism. Third, the so-called economy of gift is analyzed in the field of representative symbolism with the purpose of showing the critical function exercized by agape on the logic of equivalence. Finally, the general framework of Ricoeur's analysis of mutuality is examined in order to highlight the differences between his interpretation of asymmetry and Lévinas's version. The paper is rounded out in each of the four steps with a brief discussion of other phenomenological viewpoints that shed light on Ricoeur's claims.

  8. Scientific basis for use of Pyrus pashia Buch.-Ham. ex D. Don. fruit in gastrointestinal, respiratory and cardiovascular ailments.

    Directory of Open Access Journals (Sweden)

    Khalid Hussain Janbaz

    Full Text Available Pyrus pashia Buch.-Ham. ex D. Don. has been used conventionally by many communities in the Himalayan region for the management of gastrointestinal, respiratory, and vascular complications. Set against this background, this study was carried out to justify the scientific basis to validate folkloric uses of fruits of Pyrus pashia Buch.-Ham. ex D. Don. (Pp.Cr in traditional systems of medicine.The crude ethanol extract of fruits of Pyrus pashia Buch.-Ham. ex D. Don. (Pp.Cr was tested in vitro on isolated rabbit jejunum, tracheal, and aorta preparations. The responses of tissues were recorded using isotonic transducers coupled with a PowerLab data acquisition system.The Pp.Cr on application (0.01-5.0 mg/ml to isolated rabbit jejunum preparation exhibited relaxation through decrease in magnitude and frequency of spontaneous contractions. The Pp.Cr also exerted a relaxant (0.01-5.0 mg/ml effect on K+ (80 mM induced contractions in isolated rabbit jejunum preparations and caused shifting of the Ca2+ curves (1.0-3.0 mg/ml toward right in a manner similar to that of verapamil (3 μM, possibly suggesting presence of Ca2+ channel blocking activity. Subsequently, Pp.Cr in a concentration-dependent fashion (0.01-10.0 mg/ml caused relaxation of CCh (1 μM and K+ (80 mM induced contractions in isolated rabbit tracheal preparations in a manner comparable to that of dicyclomine, suggesting that the observed relaxant effect is likely to be mediated through antimuscarinic and/or Ca2+ channel blocking activities. Moreover, when evaluated against isolated rabbit aortic preparations, the Pp.Cr in concentrations up to 10 mg/ml exhibited a contractile response that was found to be abolished subsequent to pretreatment of isolated tissue preparation with cyproheptadine (1 μM, phentolamine (1 μM, and losartan (1 μM, suggesting that Pp.Cr may have some α-adrenergic, muscarinic, serotonergic, and angiotensin II activities.The aqueous ethanolic extract of Pyrus pashia

  9. Don Juan Vélez de León, refundidor de Quevedo (a propósito del romance “Don Repollo y doña Berza”)

    OpenAIRE

    Plata-Parga, F. (Fernando)

    2004-01-01

    Se estudian las repercusiones que tiene, para la cuestión de la autoría, el descubrimiento de nuevas versiones manuscritas del romance «Don Repollo y doña Berza» (número 683 en la edición de Blecua). El romance, publicado de forma anónima en 1621, ha sido atribuido en diferentes manuscritos a Góngora, Antonio Hurtado de Mendoza y a otros autores. La inclusión del romance en el Parnaso español (1648) de González de Salas adjudicó el poema de forma concluyente a Quevedo. Sin embargo, la recient...

  10. Minerales de mena del depósito epitermal de baja sulfuración Don Sixto, Mendoza Ore minerals from Don Sixto, a low sulfidation epithermal deposit from Mendoza

    Directory of Open Access Journals (Sweden)

    Ana Cecilia Mugas Lobos

    2012-03-01

    Full Text Available El proyecto minero Don Sixto es un depósito epitermal de baja sulfuración de Au-Ag, ubicado en el bloque de San Rafael, en el SE de la provincia de Mendoza. En este depósito, cuyo recurso aurífero es próximo a las 900.000 onzas, se ha identificado una asociación de minerales de metales preciosos que incluye: uytenbogaardtita, stromeyerita, naumannita, acantita, polibasita, stützita y cervelleita. Estos minerales son escasos y se encuentran frecuentemente asociados a oro y plata de variable fineza, diseminados en vetas de cuarzo hidrotermal y en unidades volcánicas-piroclásticas de los Grupos Choiyoi y El Portillo. En este depósito se encuentra abundante pirita, con cantidades menores de arsenopirita, calcopirita, esfalerita, magnetita, hematita y, en forma subordinada, pirrotina, galena, marcasita, bornita y boulangerita, junto con calcosina, digenita y covellina. La presencia de uytenbogaardtita, stützita, cervelleita y polibasita constituye la primera mención de estos minerales para la provincia de Mendoza.The Don Sixto mining project is a low sulfidation Au-Ag deposit, with a gold resource close to 900,000 ounces, located in the San Rafael Massif, in the SE of Mendoza province. Recently, it was found a precious-metal mineral association including uy-tenbogaardtite, stromeyerite, naumannite, acanthite, polybasite, stützite and cervelleite. These minerals are scarce and are commonly related to gold and silver of variable fineness, disseminated in hydrothermal quartz veins and volcanic-pyroclastic rock units from the Choiyoi and El Portillo Group. The most common mineral is pyrite associated with subordinated arsenopyrite, chalcopyrite, sphalerite, magnetite and hematite; lesser amounts of pyrrhotite, galena, marcasite, bornite, boulangerite, chalcocite, digenite and covellite were also recognized. This is the first mention in Mendoza province for uytenbogaardtite, stützite, cervelleite and polybasite.

  11. Isolation, characterisation and antibacterial activity studies of coumarins from Rhododendron lepidotum Wall. ex G. Don, Ericaceae Estudos de isolamento, caracterização e atividade antibacteriana de cumarinas de Rhododendron lepidotum Wall. ex G. Don, Ericaceae

    Directory of Open Access Journals (Sweden)

    Shakeel-U-Rehman

    2010-12-01

    Full Text Available Six coumarins daphnin (1, daphnetin (2, daphnetin glucoside (3, rhodonetin (4, rhodonin (5 and umbelliferone (6 were isolated from the methanolic extract of Rhododendron lepidotum Wall. ex G. Don, Ericaceae (aerial part. The compounds and their acetyl derivatives were screened for antibacterial activity against Staphylococcus aureus ATCC-29213, methicillin resistant Staphylococcus aureus ATCC-15187, Escherichia coli ATCC-8739, Pseudomonas aeruginosa ATCC-9027 by microdilution method as compared to the reference ciprofloxacin. Compound 2 displayed the best antibacterial activity with MIC 125 μg/mL against S. aureus ATCC-29213 and MRSA ATCC-15187 followed by 4 which exhibited the MIC value of 250 μg/mL against all the four tested strains. All molecules showed better antibacterial activity than their acyl derivatives.Seis cumarinas dafinina (1, dafinetina (2, dafinetina glicosídeo (3, rodonetina (4, rodonina (5 e umbeliferona (6 foram isoladas do extrato metanólico das partes aéreas de Rhododendron lepidotum Wall. ex G. Don, Ericaceae. Os compostos e seus derivados acetilados foram testados para verificar sua atividade antibacteriana contra Staphylococcus aureus ATCC-29213, Escherichia coli resistente à meticilina, Staphylococcus aureus ATCC-15187, ATCC-8739, Pseudomonas aeruginosa ATCC-9027, pelo método de microdiluição, usando ciprofloxacina como referência. A substância 2 apresentou a melhor atividade antibacteriana com o MIC 125 μg/mL contra S. aureus ATCC-29213 e MRSA ATCC-15187 seguido pela substância 4, que apresentou o valor de CIM de 250 μg/mL contra as quatro cepas testadas. Todas as moléculas apresentaram melhor atividade antibacteriana do que seus derivados acetilados.

  12. Organoids: Modeling Development and the Stem Cell Niche in a Dish.

    Science.gov (United States)

    Kretzschmar, Kai; Clevers, Hans

    2016-09-26

    Organoids are three-dimensional in-vitro-grown cell clusters with near-native microanatomy that arise from self-organizing mammalian pluripotent or adult stem cells. Although monolayer stem cell cultures were established more than 40 years ago, organoid technology has recently emerged as an essential tool for both fundamental and biomedical research. For developmental biologists, organoids provide powerful means for ex vivo modeling of tissue morphogenesis and organogenesis. Here we discuss how organoid cultures of the intestine and other tissues have been established and how they are utilized as an in vitro model system for stem cell research and developmental biology. PMID:27676432

  13. Don't end up in the fields: identity construction among Mexican adolescent immigrants, their parents, and sociocontextual processes.

    Science.gov (United States)

    Maciel, Jose A; Knudson-Martin, Carmen

    2014-10-01

    This grounded theory study of 16 Mexican immigrant adolescents and 20 of their parents examines how they construct relational identities within their families, at school, with friends, and in the larger society. Results focus on a core identity bind faced by the adolescents: immigration messages from parents that say, "don't be like me" and the societal message, "you're not like us." Response to this bind was guided by two contrasting sets of identity narratives: Empowering narratives invited an intentional approach to school and life choices. Restricting narratives maintained an ambivalent approach to school and life choices. Resolution of the identity bind was a collective, ongoing process that has implications for Mexican immigrant families and the professionals who work with them.

  14. A propósito de los dibujos inéditos del Instituto Valencia de Don Juan

    Directory of Open Access Journals (Sweden)

    Ocaña Martínez, José Antonio

    2001-06-01

    Full Text Available El Instituto Valencia de Don Juan, constituido notarialmente en 1916 conforme a las ideas de Guillermo Joaquín de Osma y Scull (La Habana, 1853-Madrid, 1922, casado con la última condesa de dicho título, Adelaida Crooke y Guzman (Madrid, 1863-París, 1918, guarda, en un reformado palacete de la calle Fortuny de Madrid, importantes colecciones de pinturas, esculturas, tapices, bordados, armas, azulejos, loza, alfarería, azabaches, monedas, muebles, libros y documentos, que el matrimonio logró reunir, provenientes de sus antepasados y adquiridos por ellos. Entre tan valiosos fondos casi pasan desapercibidos los veintiocho dibujos, que hemos catalogado recientemente, los cuales no pueden considerarse una colección sino un conjunto de piezas que llegaron aleatoriamente a la Institución, la mayoría de procedencia hoy desconocida, realizados entre principios de los siglos XVI y XX.…

  15. Don Draper, avatares e twittertainment: o comportamento dos fãs de TV na era transmidiática

    OpenAIRE

    Neves, Sheron

    2011-01-01

    O artigo propõe examinar o comportamento dos fãs de TV no atual ambiente transmidiático, usando como referência a série norte-americana Mad Men e a transformação do personagem Don Draper em celebridade. Pretendo examinar o impacto das transformações culturais sobre as estratégias de marketing das empresas produtoras de conteúdos, já que, à medida que os meios convergem, os fãs se apropriam de personagens e marcas. À medida que os fãs são constantemente convidados a imergir em universos imagin...

  16. Morphobiochemical Variability and Selection Strategies for the Germplasm of Dactylorhiza hatagirea (D. Don Soo: An Endangered Medicinal Orchid

    Directory of Open Access Journals (Sweden)

    R. S. Chauhan

    2014-01-01

    Full Text Available Dactylorhiza hatagirea (D. Don Soo (Orchidaceae is an important endangered medicinal herb, distributed in subalpine to alpine regions of the Himalayas. Its tubers are important constituents of many medicines and health tonics. Overexploitation for medicinal uses has decreased availability in natural habitats and this species has been enlisted as endangered, making conservation and cultivation studies necessary. Variability studies may serve as an important tool for effective conservation and for a crop improvement program. Therefore, natural populations of D. hatagirea were analyzed for variability on the basis of morphological, biochemical, and isoenzyme patterns. The studied populations were grouped into two clusters. Existing variability among different populations opens up new areas for conservation and perspectives for a genetic improvement program for D. hatagirea.

  17. UN FISCAL AL SERVICIO DE SU MAJESTAD: DON FRANCISCO DE ALFARO EN LA REAL AUDIENCIA DE CHARCAS, 1598-1608

    Directory of Open Access Journals (Sweden)

    M. CAROLINA JURADO

    2014-01-01

    Full Text Available Enmarcado en el ejercicio de la justicia colonial del licenciado don Francisco de Alfaro, este trabajo ofrece un análisis de su desempeño como fi scal de la Real Audiencia de Charcas, entre los años 1598 y 1608. Específi camente, se aborda su experiencia en el pleito por la concesión fraudulenta de tierras del repartimiento de Macha (norte de Potosí, durante el proceso de la Primera Visita y Composición de tierras (1591-1595 en Charcas. A partir de documentación inédita, se discute el impacto que su actuación en relación a la Real Hacienda y a redes clientelares en torno a diversos magistrados locales, así como su aprendizaje de la territorialidad indígena andina, tuvieron en su desempeño posterior.

  18. Un fiscal al servicio de su majestad: don Francisco de Alfaro en la Real Audiencia de Charcas, 1598-1608

    Directory of Open Access Journals (Sweden)

    Carolina Jurado

    2014-07-01

    Full Text Available Enmarcado en el ejercicio de la justicia colonial del licenciado don Francisco de Alfaro, este trabajo ofrece un análisis de su desempeño como fiscal de la Real Audiencia de Charcas, entre los años 1598 y 1608. Específicamente, se aborda su experiencia en el pleito por la concesión fraudulenta de tierras del repartimiento de Macha (norte de Potosí, durante el proceso de la Primera Visita y Composición de tierras (1591-1595 en Charcas. A partir de documentación inédita, se discute el impacto que su actuación en relación a la Real Hacienda y a redes clientelares en torno a diversos magistrados locales, así como su aprendizaje de la territorialidad indígena andina, tuvieron en su desempeño posterior. 

  19. Living in a simulacrum: how TV and the supermarket redefines reality in Don DeLillo’s White Noise

    Directory of Open Access Journals (Sweden)

    Ghashmari, Ahmad

    2010-07-01

    Full Text Available This paper examines the impact of simulation, hyperreality, and consumerism on Don Delillo's novel "White Noise". It discusses how the novel pictures technology and mass media as an empire of signs and codes that erase or implode meaning. TV, radio reports and tidbits, and medical imaging devices are intertwined with many aspects of people's lives in this late capitalist culture. Futhermore, the paper will shed some light on the issue of hyperreality which is generated by simulations. We will see how this new type of reality becomes more real than reality itself. Then, the influence of the supermarket and the emergence of consumer culture will be discussed. We will see how production and consumption have gained a new different meaning in this new "superficial" society and how it reshapes people's undestanding and interaction with reality.

  20. Geological and environmental observations in the Sunchales Creek and Tonco River , Don Otto uranium mine, Salta Province

    International Nuclear Information System (INIS)

    Results of geological and environmental observations made by professionals of the Comisión Nacional de Energía Atómica (CNEA) down streams Don Otto uranium mine on the Sunchales creek, from Los Guanacos strait down to the Tonco river mouth, and from river mouth down to narrow pass named Salto Grande, are presented in this report. La Yesera, Las Curtiembres and Los Blanquitos Formations of the Pirgua Subgroup, and Lecho and Yacoraite Formations of the Balbuena Subgroup crops out in that area (Salta Group, Cretaceous- Middle Eocene). Water stream analyses have given uranium contents between 890 μg/L and 1,100 μg/L, and uranium in stream sediments between 4 μg/g and 14 μg/g; radimetric anomalias up to 1,500 c/s in discoloured facies of the Las Curtiembres Formation, and 2,000 c/s in dark pelitic sediments of the Yacoraite Formation. A new zone with uranium traces in Curtiembres Formation belonging to the Pirgua Subgroup has been identified in the Alemanía cretaceous basin, which is stratigraphically similar to Cajoncillo, Las Abritas and El Fraile deposits. Those traces in addition to the other ones localized in the upper third of the Yacoraite Formation would have been originated by the geochemical uranium anomalies in waters and stream sediments by uranium leaching of rocks. The geochemical uranium anomalies in water and sediments are not a consequence of the antropic activity of the uranium mining carried out in Don Otto mine (1961-1982). (authors)

  1. LA PARODIA ENTRE LA FICCIÓN YLA REALIDAD EN EL INGENIOSO HIDALGO DON QUIJOTE DE LA MANCHA

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    Nora González Gandiaga

    2005-11-01

    Full Text Available En este trabajo se aborda la problemática de la ficción y de la realidad intermediada por la operación paródica en El Ingenioso Hidalgo don Quijote de La Mancha de Miguel de Cervantes Saavedra. Se asedia el texto para ver cómo la parodia y la ironía son estrategias fundamentales para leer la concepción novelesca y cómo se permite al lector avieso adentrarse en la Poética cervantina, partiendo de los paratextos: prólogo, sonetos iniciales antes de adentrarnos en el cuerpo de la novela para luego calar en éste. En todo este cometido colaboran los narradores ficticios para "leer" la novela moderna que postula Cervantes en el libro e incluso se advierte que los distintos narradores dejan al descubierto la parodia y al mismo tiempo permiten un juego crítico-reflexivo en la materia textualThis paper examines the problematic of fiction and reality as mediated by parody in Cervantes’ Don Quijote de la Mancha. The approach to the text is meant to see how parody and irony become fundamental strategies to understand the novelistic concept, and how the perceptive reader is permitted to enter into the Cervantine poetic, starting from the paratexts: the Prologue; the opening sonnets, before getting into the core of the work discriminatingly. In all this procedure fictitious narrators collaborate in the reading of the modernistic novel which Cervantes postulated. It may also be noticed how the different narrators disclose the parodic while allowing a critical-reflexive play in the text

  2. Antiproliferative and Apoptosis Induction Potential of the Methanolic Leaf Extract of Holarrhena floribunda (G. Don

    Directory of Open Access Journals (Sweden)

    J. A. Badmus

    2015-01-01

    Full Text Available Natural plant products with potent growth inhibition and apoptosis induction properties are extensively being investigated for their cancer chemopreventive potential. Holarrhena floribunda (HF is used in a wide range of traditional medicine practices. The present study investigated the antiproliferative and apoptosis induction potential of methanolic leaf extracts of HF against breast (MCF-7, colorectal (HT-29, and cervical (HeLa cancer cells relative to normal KMST-6 fibroblasts. The MTT assay in conjunction with the trypan blue dye exclusion and clonogenic assays were used to determine the effects of the extracts on the cells. Caspase activities were assayed with Caspase-Glo 3/7 and Caspase-9 kits. Apoptosis induction was monitored by flow cytometry using the APOPercentage and Annexin V-FITC kits. Reactive oxygen species (ROS was measured using the fluorogenic molecular probe 5-(and-6-chloromethyl-2′,7′-dichlorofluorescein diacetate acetyl ester and cell cycle arrest was detected with propidium iodide. Dose-response analyses of the extract showed greater sensitivity in cancer cell lines than in fibroblast controls. Induction of apoptosis, ROS, and cell cycle arrest were time- and dose-dependent for the cancer cell lines studied. These findings provide a basis for further studies on the isolation, characterization, and mechanistic evaluation of the bioactive compounds responsible for the antiproliferative activity of the plant extract.

  3. Peut-on penser le travail par le don ? Débat avec la rédaction de la Nouvelle Revue du Travail Conceptualising work as a gift? Debate with editors of the Nouvelle Revue du Travail ¿Es posible pensar el trabajo a través del don? Debate con el comité de redacción de la Nouvelle Revue du Travail

    OpenAIRE

    Alain Caillé; Norbert Alter

    2012-01-01

    Dans un échange avec la Nouvelle Revue du Travail, Alain Caillé et Norbert Alter soutiennent leur lecture maussienne des réalités contemporaines du travail en discutant les questions centrales du capitalisme, du salariat, de l’entreprise et du travail sociologique. Cet échange fait suite aux contributions d’Alain Caillé et Norbert Alter dans ce Chorus « Peut-on penser le travail par le don ? De la mise en œuvre du paradigme du don contre-don en sociologie du travail. »In an exchange with the ...

  4. Amperometric biosensor with nanostructured electrodes by using multi-alled carbon nanotubes for glucose detection in cell culture medium

    OpenAIRE

    Boero, Cristina; Carrara, Sandro; De Micheli, Giovanni

    2009-01-01

    The monitoring of metabolic compounds such as glucose is largely reported in literature. The applications of this type of analysis are mainly related to clinical purposes, e.g. in diabetes pathology, where a lot of studies are presented in literature. Recently, some authors presented studies about glucose and lactate detection in cell culture monitoring [1], [2]. A clear identification of medium compounds could be interesting for biologists and biotechnologists, since they may be identified a...

  5. Final Environmental Assessment : Opening waterfowl hunting on 5,500 acres of former commercial salt ponds : Don Edwards San Francisco Bay National Wildlife Refuge : Alviso and Ravenswood Salt Ponds

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This EA for opening waterfowl hunting on salt ponds recently acquired by the Don Edwards San Francisco Bay National Wildlife Refuge proposes three alternatives....

  6. Le don en anamorphose ou la réécriture du monde. Configurations et enjeux de l’hospitalité dans le récit médiéval (XIIe-XIIIe siècles

    Directory of Open Access Journals (Sweden)

    Carlos Fonseca Clamote Carreto

    2009-05-01

    Full Text Available  « A la limite, le don comme don devrait ne pas apparaître comme don ni au donataire, ni au donateur […]. En ce sens, le don est l’impossible. Non pas impossible, mais la figure même de l’impossible. » J. Derrida, Donner le temps.« Biaz sire, fet il, bien vaingniez.Se o moi herbergiez daingniez,Vez l’ostel aparelliés »Chrétien de Troyes, Érec et Énide.Arthur ou la largesse infinieÀ une époque où, de Jean de Salisbury à Brunetto Latini, en passant par Jacques de Vitry ou Guillaume Le Clerc de ...

  7. Cancer cell differentiation heterogeneity and aggressive behavior in solid tumors.

    Science.gov (United States)

    Jögi, Annika; Vaapil, Marica; Johansson, Martin; Påhlman, Sven

    2012-05-01

    The differentiation stage of tumors is a central aspect in the histopathological classification of solid malignancies. The differentiation stage is strongly associated with tumor behavior, and generally an immature tumor is more aggressive than the more differentiated counterpart. While this is common knowledge in surgical pathology, the contribution of differentiation-related gene expression and functions to tumor behavior is often overlooked in the experimental, tumor biological setting. The mechanisms by which tumor cell differentiation stages are perturbed or affected are poorly explored but have recently come into focus with the introduction.of the tumor stem cell concept. While developmental biologists view the differentiation as a unidirectional event, pathologists and tumor biologists have introduced the concept of dedifferentiation to explain phenotypic changes occurring in solid tumors. In this review we discuss the impact of the tumor cell differentiation stage as used in surgical pathology. We further discuss knowledge gained from exploring the molecular basis of the differentiation and dedifferentiation processes in neuroblastoma and breast cancer, two tumor forms where the tumor cell differentiation concept is used in the clinical diagnostic work and where the tumor stem cell theory has been applied.

  8. Polycyclic aromatic hydrocarbon levels and measures of oxidative stress in the Mediterranean endemic bivalve Pinna nobilis exposed to the Don Pedro oil spill.

    Science.gov (United States)

    Sureda, Antoni; Tejada, Silvia; Box, Antonio; Deudero, Salud

    2013-06-15

    The fan mussel (Pinna nobilis Linné, 1758) is the largest endemic Mediterranean bivalve subject to strict protection as an endangered species. Antioxidant biomarkers in P. nobilis gills for biomonitoring marine pollution were researched after the Don Pedro oil spill. Two sampling locations on the east and southeast of the island of Ibiza (Western Mediterranean, Spain) were selected, one extensively affected by the oil spill and the other unaffected (control area). Mussels were sampled 1 month, 6 months and 1 year after the accident. Polycyclic aromatic hydrocarbon levels and antioxidant enzymes significantly increased as result of the oil spill in all sampling periods (p<0.05). Oxidative damage in lipids significantly increased in the mussels collected in the affected area (p<0.05), though such damage was back to normal after 1 year. In conclusion, the Don Pedro oil spill induced a situation of oxidative stress on P. nobilis that continued a year later. PMID:23623655

  9. Savez-vous à qui vous avez affaire ? De la vengeance au don, de l’offense au pardon

    Directory of Open Access Journals (Sweden)

    Mark R. Anspach

    2009-02-01

    Full Text Available Pardonner, c’est renoncer au désir de se venger, c’est donc éviter de s’engager dans le cercle de la réciprocité négative. Or, il est difficile d’échapper à ce cercle vicieux sans accepter d’entrer dans le cercle vertueux de la réciprocité positive : voilà du moins la thèse que nous avons soutenue dans un premier travail sur la violence et le don. Nous nous proposons ici de partir de cette même thèse pour entamer une nouvelle réflexion sur le pardon. Comment le passage de la vengeance au don ...

  10. Electron Tomography in Plant Cell Biology

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This review focuses on the contribution of electron tomography-based techniques to our understanding of cellular processes in plant cells. Electron microscopy techniques have evolved to provide better three-dimensional resolution and improved preservation of the subcellular components. In particular, the combination of cryofixation/freeze substitution and electron tomography have allowed plant cell biologists to image organelles and macromolecular complexes in their native cellular context with unprecedented three-dimensional resolution (4-7 nm). Until now, electron tomography has been applied in plant cell biology for the study of cytokinesis, Golgi structure and trafficking, formation of plant endosome/prevacuolar compartments, and organization of photosynthetic membranes. We discuss in this review the new insights that these tomographic studies have brought to the plant biology field.

  11. "It sure as hell looked like war": terrorism and the Cold War in Thomas Pynchon's Against the Day and Don DeLillo's Underworld

    OpenAIRE

    Eve, Martin Paul

    2013-01-01

    This piece explores, necessarily briefly, the conceptions of terrorism in two novels that stand separated by the calamitous events of September 11th, 2001: Pynchon's Against the Day and Don DeLillo's Underworld, with special focus upon the genesis of these depictions in Cold War politics. While there are cases to be made for many geographico-historical connections in both Pynchon's and DeLillo's work ? for instance, Sam Thomas has recently highlighted the Balkans ? the Cold War presents a loc...

  12. Short-term and long-term effects of tannins on nitrogen mineralisation and litter decomposition in kauri (Agathis australis (D. Don) Lindl.) forests

    OpenAIRE

    Verkaik, E.; Jongkind, A.G.; Berendse, F.

    2006-01-01

    Kauri (Agathis australis (D. Don) Lindl.) occurs naturally in the warm temperate forest of northern New Zealand where it grows mixed with angiosperm tree species. Below mature kauri trees thick organic layers develop in which large amounts of nitrogen are accumulated. This nitrogen seems to be inaccessible to plants. While litter quality can explain the low decomposition rate below kauri, it is not known what causes the accumulation of nitrogen. We hypothesised that kauri tannins reduce nitro...

  13. USING MINDMAPPING TECHNIQUE TO INCREASE THE ABILITY IN WRITING NEWS REPORT AT THE ELEVENTH GRADE STUDENTS OF IPA AT SMA FRATER. DON BOSCO

    OpenAIRE

    Jerry Mias Rofinus

    2011-01-01

    Jerry Mias Rofinus 2011. Using Mind Mapping technique to Increase the Ability in Writing News Report at the Eleventh Grade Students of IPA at SMA Frater Don Bosco Tarakan. Pembimbing: (1) Yansar, S.S., M. Pd (2) Ridwan, M. Pd) In learning process, there were domains that expected could help in achievement by the objective learning. The three of domain are cognitive, affective and psychometric. The focus given to third domain and expected can improve performance by students. In fact, in lea...

  14. Stratigraphic and tectonic notes on the Villafranca d'Asti succession in type-area and Castelnuovo Don Bosco sector (ASTI reliefs, Piedmont)

    OpenAIRE

    Forno, GM; GATTIGLIO, M.; C. Comina; BARBERO, D.; Bertini, A; Doglione, A; F. Gianotti; Irace, A.; Martinetto, E.; Mottura, A; Sala, B.

    2015-01-01

    Interest in the sedimentary succession in the Asti Reliefs fostered research since the beginning of the science of geology, before the designation by Pareto (1865) of the "Piano Villafranchiano" and its type area at Villafranca d'Asti. The villafranchian sediments have been the subject of many recent investigations, which focused largely on the palaeontology of vertebrates and plant remains. Geological surveys in the type area and in the Castelnuovo don Bosco sector revealed that the sediment...

  15. Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don

    OpenAIRE

    Shi-Hai Xing; Xin-Bo Guo; Quan Wang; Qi-Fang Pan; Yue-Sheng Tian; Pin Liu; Jing-Ya Zhao; Guo-Feng Wang; Xiao-Fen Sun; Ke-Xuan Tang

    2011-01-01

    The tetraploid plants of Catharanthus roseus (L.) G. Don was obtained by colchicine induction from seeds explants, and the ploidy of the plants was identified by flow cytometry. The optimal treatment is 0.2% colchicine solution treated for 24 hours, and the induction rate reaches up to 30%. Comparing with morphological characteristics and growth habits between tetraploids and the control, we found that tetraploids of C. roseus had larger stoma and more branches and leaves. HPLC analysis showe...

  16. Evaluation of the in vitro and in vivo antioxidant potential of whole plant extracts of Hedyotis puberula (G. Don) R. Br. ex Arn.

    OpenAIRE

    Joseph, Jince M.; Sowndhararajan, Kandhasamy; Manian, Sellamuthu

    2011-01-01

    The total phenolic content and in vitro and in vivo antioxidant activities of the whole plants of Hedyotis puberula (G. Don) R.Br. ex Arn. were appraised. The methanol extract of the plant contained higher levels of total phenolics, tannins and flavonoids content than other solvent extracts. Extracts were screened for antioxidant and free radical scavenging activities using various in vitro model systems. The methanol extract manifested strongest antioxidant and free radical scave...

  17. Epidemiology of Ancylostoma spp. in the endangered Iberian lynx (Lynx pardinus) in the Don˜ ana National Park, south-west Spain

    OpenAIRE

    Vicente, J. de; Palomares, Francisco; Ruíz de Ibáñez, R.; Ortíz, J.

    2004-01-01

    The epidemiology of Ancylostoma spp. was studied in the endangered Iberian lynx (Lynx pardinus) in the Don˜ ana National Park, south-west Spain. Faecal samples were collected throughout a complete annual cycle (August 1997 to September 1998). The overall egg prevalence of Ancylostoma spp. was 57.8%. The pattern of abundance of Ancylostoma spp. eggs in faeces was overdispersed. Juvenile lynx demonstrated a statistically higher prevalence and abundance of Ancylostoma spp. than i...

  18. Suitport Feasibility - Development and Test of a Suitport and Space Suit for Human Pressurized Space Suit Donning Tests

    Science.gov (United States)

    Boyle, Robert M.; Mitchell, Kathryn; Allton, Charles; Ju, Hsing

    2011-01-01

    The suitport concept has been recently implemented as part of the small pressurized lunar rover (Currently the Space Exploration vehicle, or SEV) and the Multi-Mission Space Exploration Vehicle (MMSEV) concept demonstrator vehicle. Suitport replaces or augments the traditional airlock function of a spacecraft by providing a bulkhead opening, capture mechanism, and sealing system to allow ingress and egress of a spacesuit while the spacesuit remains outside of the pressurized volume of the spacecraft. This presents significant new opportunities to EVA exploration in both microgravity and surface environments. The suitport concept will enable three main improvements in EVA by providing reductions in: pre-EVA time from hours to less than thirty minutes; airlock consumables; contamination returned to the cabin with the EVA crewmember. To date, the first generation suitport has been tested with mockup suits on the rover cabins and pressurized on a bench top engineering unit. The work on the rover cabin has helped define the operational concepts and timelines, and has demonstrated the potential of suitport to save significant amounts of crew time before and after EVAs. The work with the engineering unit has successfully demonstrated the pressurizable seal concept including the ability to seal after the introduction and removal of contamination to the sealing surfaces. Using this experience, a second generation suitport was designed. This second generation suitport has been tested with a spacesuit prototype using the pressure differentials of the spacecraft. This test will be performed using the JSC B32 Chamber B, a human rated vacuum chamber. This test will include human rated suitports, the suitport compatible prototype suit, and chamber modifications. This test will bring these three elements together in the first ever pressurized donning of a rear entry suit through a suitport. This paper presents design of a human rated second generation suitport, modifications to

  19. Suitport Feasibility: Development and Test of a Suitport and Space Suit for Human Pressurized Space Suit Donning Tests

    Science.gov (United States)

    Boyle, Robert M.; Mitchell, Kathryn; Allton, Charles; Ju, Hsing

    2012-01-01

    The suitport concept has been recently implemented as part of the small pressurized lunar rover (Currently the Space Exploration vehicle, or SEV) and the Multi-Mission Space Exploration Vehicle (MMSEV) concept demonstrator vehicle. Suitport replaces or augments the traditional airlock function of a spacecraft by providing a bulkhead opening, capture mechanism, and sealing system to allow ingress and egress of a space suit while the space suit remains outside of the pressurized volume of the spacecraft. This presents significant new opportunities to EVA exploration in both microgravity and surface environments. The suitport concept will enable three main improvements in EVA by providing reductions in: pre-EVA time from hours to less than thirty minutes; airlock consumables; contamination returned to the cabin with the EVA crewmember. To date, the first generation suitport has been tested with mockup suits on the rover cabins and pressurized on a bench top engineering unit. The work on the rover cabin has helped define the operational concepts and timelines, and has demonstrated the potential of suitport to save significant amounts of crew time before and after EVAs. The work with the engineering unit has successfully demonstrated the pressurizable seal concept including the ability to seal after the introduction and removal of contamination to the sealing surfaces. Using this experience, a second generation suitport was designed. This second generation suitport has been tested with a space suit prototype on the second generation MMSEV cabin, and testing is planned using the pressure differentials of the spacecraft. Pressurized testing will be performed using the JSC B32 Chamber B, a human rated vacuum chamber. This test will include human rated suitports, a suitport compatible prototype suit, and chamber modifications. This test will bring these three elements together in the first ever pressurized donning of a rear entry suit through a suitport. This paper presents

  20. Cells Sensing Mechanical Cues: Stiffness Influences the Lifetime of Cell-Extracellular Matrix Interactions by Affecting the Loading Rate.

    Science.gov (United States)

    Jiang, Li; Sun, Zhenglong; Chen, Xiaofei; Li, Jing; Xu, Yue; Zu, Yan; Hu, Jiliang; Han, Dong; Yang, Chun

    2016-01-26

    The question of how cells sense substrate mechanical cues has gained increasing attention among biologists. By introducing contour-based data analysis to single-cell force spectroscopy, we identified a loading-rate threshold for the integrin α2β1-DGEA bond beyond which a dramatic increase in bond lifetime was observed. On the basis of mechanical cues (elasticity or topography), the effective spring constant of substrates k is mapped to the loading rate r under actomyosin pulling speed v, which, in turn, affects the lifetime of the integrin-ligand bond. Additionally, downregulating v with a low-dose blebbistatin treatment promotes the neuronal lineage specification of mesenchymal stem cells on osteogenic stiff substrates. Thus, sensing of the loading rate is central to how cells sense mechanical cues that affect cell-extracellular matrix interactions and stem cell differentiation.