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Sample records for cell biologist don

  1. Human radiation studies: Remembering the early years: Oral history of cell biologist Don Francis Petersen, Ph.D., conducted November 29, 1994

    International Nuclear Information System (INIS)

    This report is a transcript of an interview of Dr. Don Francis Petersen by representatives of the US DOE Office of Human Radiation Experiments. Dr. Petersen was selected for this interview because of his long research career at Los Alamos and his knowledge of the Atomic Energy Commission's biomedical program. Dr. Petersen did not personally conduct research on human subjects. After a brief biographical sketch Dr. Petersen discusses his remembrances of the early use of radionuclides as biological tracers, aspects of nuclear weapons testing in the 1940's and 1950's including fallout studies, the means by which research projects were approved, use of humans in the whole-body counter, and the Health Division Biomedical responsibilities

  2. Human radiation studies: Remembering the early years: Oral history of cell biologist Don Francis Petersen, Ph.D., conducted November 29, 1994

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1995-08-01

    This report is a transcript of an interview of Dr. Don Francis Petersen by representatives of the US DOE Office of Human Radiation Experiments. Dr. Petersen was selected for this interview because of his long research career at Los Alamos and his knowledge of the Atomic Energy Commission`s biomedical program. Dr. Petersen did not personally conduct research on human subjects. After a brief biographical sketch Dr. Petersen discusses his remembrances of the early use of radionuclides as biological tracers, aspects of nuclear weapons testing in the 1940`s and 1950`s including fallout studies, the means by which research projects were approved, use of humans in the whole-body counter, and the Health Division Biomedical responsibilities.

  3. Thousands of rab GTPases for the cell biologist.

    Directory of Open Access Journals (Sweden)

    Yoan Diekmann

    2011-10-01

    Full Text Available Rab proteins are small GTPases that act as essential regulators of vesicular trafficking. 44 subfamilies are known in humans, performing specific sets of functions at distinct subcellular localisations and tissues. Rab function is conserved even amongst distant orthologs. Hence, the annotation of Rabs yields functional predictions about the cell biology of trafficking. So far, annotating Rabs has been a laborious manual task not feasible for current and future genomic output of deep sequencing technologies. We developed, validated and benchmarked the Rabifier, an automated bioinformatic pipeline for the identification and classification of Rabs, which achieves up to 90% classification accuracy. We cataloged roughly 8.000 Rabs from 247 genomes covering the entire eukaryotic tree. The full Rab database and a web tool implementing the pipeline are publicly available at www.RabDB.org. For the first time, we describe and analyse the evolution of Rabs in a dataset covering the whole eukaryotic phylogeny. We found a highly dynamic family undergoing frequent taxon-specific expansions and losses. We dated the origin of human subfamilies using phylogenetic profiling, which enlarged the Rab repertoire of the Last Eukaryotic Common Ancestor with Rab14, 32 and RabL4. Furthermore, a detailed analysis of the Choanoflagellate Monosiga brevicollis Rab family pinpointed the changes that accompanied the emergence of Metazoan multicellularity, mainly an important expansion and specialisation of the secretory pathway. Lastly, we experimentally establish tissue specificity in expression of mouse Rabs and show that neo-functionalisation best explains the emergence of new human Rab subfamilies. With the Rabifier and RabDB, we provide tools that easily allows non-bioinformaticians to integrate thousands of Rabs in their analyses. RabDB is designed to enable the cell biology community to keep pace with the increasing number of fully-sequenced genomes and change the scale

  4. Charged Particles are Prevented from Going Faster than the Speed of Light by Light Itself: A Biophysical Cell Biologist's Contribution to Physics

    OpenAIRE

    Wayne, Randy

    2011-01-01

    Investigations of living organisms have led biologists and physicians to introduce fundamental concepts, including Brownian motion, the First Law of Thermodynamics, Poiseuille's Law of fluid flow, and Fick's Law of diffusion into physics. Given the prominence of viscous forces within and around cells and the experience of identifying and quantifying such resistive forces, biophysical cell biologists have an unique perspective in discovering the viscous forces that cause moving particles to re...

  5. GeneSpeed Beta Cell: An Online Genomics Data Repository and Analysis Resource Tailored for the Islet Cell Biologist

    Directory of Open Access Journals (Sweden)

    Jan Jensen

    2008-09-01

    Full Text Available Objective. We here describe the development of a freely available online database resource, GeneSpeed Beta Cell, which has been created for the pancreatic islet and pancreatic developmental biology investigator community. Research Design and Methods. We have developed GeneSpeed Beta Cell as a separate component of the GeneSpeed database, providing a genomics-type data repository of pancreas and islet-relevant datasets interlinked with the domain-oriented GeneSpeed database. Results. GeneSpeed Beta Cell allows the query of multiple published and unpublished select genomics datasets in a simultaneous fashion (multiexperiment viewing and is capable of defining intersection results from precomputed analysis of such datasets (multidimensional querying. Combined with the protein-domain categorization/assembly toolbox provided by the GeneSpeed database, the user is able to define spatial expression constraints of select gene lists in a relatively rigid fashion within the pancreatic expression space. We provide several demonstration case studies of relevance to islet cell biology and development of the pancreas that provide novel insight into islet biology. Conclusions. The combination of an exhaustive domain-based compilation of the transcriptome with gene array data of interest to the islet biologist affords novel methods for multidimensional querying between individual datasets in a rapid fashion, presently not available elsewhere.

  6. Charged Particles are Prevented from Going Faster than the Speed of Light by Light Itself: A Biophysical Cell Biologist's Contribution to Physics

    CERN Document Server

    Wayne, Randy

    2011-01-01

    Investigations of living organisms have led biologists and physicians to introduce fundamental concepts, including Brownian motion, the First Law of Thermodynamics, Poiseuille's Law of fluid flow, and Fick's Law of diffusion into physics. Given the prominence of viscous forces within and around cells and the experience of identifying and quantifying such resistive forces, biophysical cell biologists have an unique perspective in discovering the viscous forces that cause moving particles to respond to an applied force in a nonlinear manner. Using my experience as a biophysical cell biologist, I show that in any space consisting of a photon gas with a temperature above absolute zero, Doppler-shifted photons exert a velocity-dependent viscous force on moving charged particles. This viscous force prevents charged particles from exceeding the speed of light. Consequently, light itself prevents charged particles from moving faster than the speed of light. This interpretation provides a testable alternative to the i...

  7. Frequent biphasic cellular responses of permanent fish cell cultures to deoxynivalenol (DON)

    International Nuclear Information System (INIS)

    Contamination of animal feed with mycotoxins is a major problem for fish feed mainly due to usage of contaminated ingredients for production and inappropriate storage of feed. The use of cereals for fish food production further increases the risk of a potential contamination. Potential contaminants include the mycotoxin deoxynivalenol (DON) which is synthesized by globally distributed fungi of the genus Fusarium. The toxicity of DON is well recognized in mammals. In this study, we confirm cytotoxic effects of DON in established permanent fish cell lines. We demonstrate that DON is capable of influencing the metabolic activity and cell viability in fish cells as determined by different assays to indicate possible cellular targets of this toxin. Evaluation of cell viability by measurement of membrane integrity, mitochondrial activity and lysosomal function after 24 h of exposure of fish cell lines to DON at a concentration range of 0-3000 ng ml-1 shows a biphasic effect on cells although differences in sensitivity occur. The cell lines derived from rainbow trout are particularly sensitive to DON. The focus of this study lies, furthermore, on the effects of DON at different concentrations on production of reactive oxygen species (ROS) in the different fish cell lines. The results show that DON mainly reduces ROS production in all cell lines that were used. Thus, our comparative investigations reveal that the fish cell lines show distinct species-related endpoint sensitivities that also depend on the type of tissue from which the cells were derived and the severity of exposure. - Highlights: → DON uptake by cells is not extensive. → All fish cell lines are sensitive to DON. → DON is most cytotoxic to rainbow trout cells. → Biphasic cellular responses were frequently observed. → Our results are similar to studies on mammalian cell lines.

  8. Easy mathematics for biologists

    CERN Document Server

    Foster, Peter C

    2014-01-01

    Because elementary mathematics is vital to be able to properly design biological experiments and interpret their results. As a student of the life sciences you will only make your life harder by ignoring mathematics entirely. Equally, you do not want to spend your time struggling with complex mathematics that you will never use. This book is the perfect answer to your problems. Inside, it explains the necessary mathematics in easy-to-follow steps, introducing the basics and showing you how to apply these to biological situations. Easy Mathematics for Biologists covers the basic mathematical ideas of fractions, decimals and percentages, through ratio and proportion, exponents and logarithms, to straight line graphs, graphs that are not straight lines, and their transformation. Direct application of each of these leads to a clear understanding of biological calculations such as those involving concentrations and dilutions, changing units, pH, and linear and non-linear rates of reaction. Each chapter contains wo...

  9. Detection of calmodulin binding protein at 170 KDA in BALB, AKR, DON and chicken granulosa cells

    International Nuclear Information System (INIS)

    Calmodulin (CAM) has been shown to bind to the epidermal growth factor (EGF) receptor (170 kDa) and is phosphorylated in a EGF dependent manner in the A431 human epidermoid carcinoma cells. In the present study, they report 125I-CAM binding to a 170 kDa protein detected in cell membrane vesicles of Balb/3T3, AKR, DON and chicken granulosa cells. Purified plasma membranes from these cells were resolved via electrophoresis (without heat denaturation) and electroblotted onto nictrocellulose paper. Upon hybridizing against 125I-CAM, a distinct autoradiographic band occurred at 170 kDa for all the cells lines under study. The binding of CAM is specific and can be displaced with the addition of excess unlabeled CAM. The result suggest that 125I-CAM may bind to the 170 kDa EGF receptor in BALB, AKR, DON and chicken granulosa cells

  10. Transcriptome analysis of the human T lymphocyte cell line Jurkat and human peripheral blood mononuclear cells exposed to deoxynivalenol (DON): New mechanistic insights

    Energy Technology Data Exchange (ETDEWEB)

    Katika, Madhumohan R. [RIKILT-Institute of Food Safety, Wageningen University and Research Centre, Wageningen (Netherlands); Department of Health Risk Analysis and Toxicology, Maastricht University (Netherlands); Netherlands Toxicogenomics Centre (Netherlands); Hendriksen, Peter J.M. [RIKILT-Institute of Food Safety, Wageningen University and Research Centre, Wageningen (Netherlands); Netherlands Toxicogenomics Centre (Netherlands); Shao, Jia [RIKILT-Institute of Food Safety, Wageningen University and Research Centre, Wageningen (Netherlands); Department of Health Risk Analysis and Toxicology, Maastricht University (Netherlands); Netherlands Toxicogenomics Centre (Netherlands); Loveren, Henk van [Department of Health Risk Analysis and Toxicology, Maastricht University (Netherlands); National Institute for Public Health and the Environment (RIVM), Bilthoven (Netherlands); Netherlands Toxicogenomics Centre (Netherlands); Peijnenburg, Ad, E-mail: ad.peijnenburg@wur.nl [RIKILT-Institute of Food Safety, Wageningen University and Research Centre, Wageningen (Netherlands); Netherlands Toxicogenomics Centre (Netherlands)

    2012-10-01

    Deoxynivalenol (DON) or vomitoxin is a commonly encountered type-B trichothecene mycotoxin, produced by Fusarium species predominantly found in cereals and grains. DON is known to exert toxic effects on the gastrointestinal, reproductive and neuroendocrine systems, and particularly on the immune system. Depending on dose and exposure time, it can either stimulate or suppress immune function. The main objective of this study was to obtain a deeper insight into DON-induced effects on lymphoid cells. For this, we exposed the human T-lymphocyte cell line Jurkat and human peripheral blood mononuclear cells (PBMCs) to various concentrations of DON for various times and examined gene expression changes by DNA microarray analysis. Jurkat cells were exposed to 0.25 and 0.5 μM DON for 3, 6 and 24 h. Biological interpretation of the microarray data indicated that DON affects various processes in these cells: It upregulates genes involved in ribosome structure and function, RNA/protein synthesis and processing, endoplasmic reticulum (ER) stress, calcium-mediated signaling, mitochondrial function, oxidative stress, the NFAT and NF-κB/TNF-α pathways, T cell activation and apoptosis. The effects of DON on the expression of genes involved in ER stress, NFAT activation and apoptosis were confirmed by qRT-PCR. Other biochemical experiments confirmed that DON activates calcium-dependent proteins such as calcineurin and M-calpain that are known to be involved in T cell activation and apoptosis. Induction of T cell activation was also confirmed by demonstrating that DON activates NFATC1 and induces its translocation from the cytoplasm to the nucleus. For the gene expression profiling of PBMCs, cells were exposed to 2 and 4 μM DON for 6 and 24 h. Comparison of the Jurkat microarray data with those obtained with PBMCs showed that most of the processes affected by DON in the Jurkat cell line were also affected in the PBMCs. -- Highlights: ► The human T cell line Jurkat and human

  11. Phytic acid protects porcine intestinal epithelial cells from deoxynivalenol (DON) cytotoxicity.

    OpenAIRE

    Pacheco, Graziela Drociunas; Silva, Caio Abércio da; Pinton, Philippe; Oswald, Isabelle

    2012-01-01

    The purpose of this study was to evaluate the effects of phytic acid (IP(6)) as a possible inhibitor of cellular damage induced by toxic substances such as mycotoxins on a porcine intestinal epithelial cell line (IPEC-1). We first observed that a dose of 5 mM phytic acid decreases cell viability and transepithelial electrical resistance (TEER) of cell monolayer. We next investigate the effect of non-cytotoxic dose of phytic acid on the deoxinivalenol (DON) induced decreased TEER. We showed th...

  12. A novel recombinant cell fluorescence biosensor based on toxicity of pathway for rapid and simple evaluation of DON and ZEN

    Science.gov (United States)

    Ji, Jian; Gu, Wenshu; Sun, Chao; Sun, Jiadi; Jiang, Hui; Zhang, Yinzhi; Sun, Xiulan

    2016-01-01

    During an exposure, humans and animals are most often exposed to a mixture rather than individual mycotoxins. In this study, a Human Embryonic Kidney 293 cell (HEK-293) fluorescence sensor was developed to detect and evaluate mycotoxins, deoxynivalenol (DON) and zearalenone (ZEN) compounds, produced by Fusarium culmorum that are common food contaminants. TRE-copGFP (green fluorescent protein) and ERE-TagRFP (red fluorescent protein) plasmids were constructed and cotransfected into HEK-293 cells through a highly efficient, lipid-mediated, DNA-transfection procedure. Results show that fluorescence intensity was proportional to DON and ZEN concentrations, ranging from 2 to 40 ng/mL and 10 to 100 ng/mL respectively, with a detection limit of 0.75 ng/mL and 3.2 ng/mL respectively. The EC50 of DON and ZEN are 30.13 ng/mL and 76.63 ng/mL respectively. Additionally, ZEN may have a synergistic effect on enhancing AP-1 activity of the toxicity pathway of DON. These data indicate the high sensitivity and effectiveness of our biosensor system in the evaluation of the combined toxicity of ZEN, DON and their derivatives. In addition, this approach is suitable for an early warning method for the detection of ZEN and DON family mycotoxins contamination without higher-priced, conventional analytical chemistry methods. PMID:27498557

  13. A novel recombinant cell fluorescence biosensor based on toxicity of pathway for rapid and simple evaluation of DON and ZEN.

    Science.gov (United States)

    Ji, Jian; Gu, Wenshu; Sun, Chao; Sun, Jiadi; Jiang, Hui; Zhang, Yinzhi; Sun, Xiulan

    2016-01-01

    During an exposure, humans and animals are most often exposed to a mixture rather than individual mycotoxins. In this study, a Human Embryonic Kidney 293 cell (HEK-293) fluorescence sensor was developed to detect and evaluate mycotoxins, deoxynivalenol (DON) and zearalenone (ZEN) compounds, produced by Fusarium culmorum that are common food contaminants. TRE-copGFP (green fluorescent protein) and ERE-TagRFP (red fluorescent protein) plasmids were constructed and cotransfected into HEK-293 cells through a highly efficient, lipid-mediated, DNA-transfection procedure. Results show that fluorescence intensity was proportional to DON and ZEN concentrations, ranging from 2 to 40 ng/mL and 10 to 100 ng/mL respectively, with a detection limit of 0.75 ng/mL and 3.2 ng/mL respectively. The EC50 of DON and ZEN are 30.13 ng/mL and 76.63 ng/mL respectively. Additionally, ZEN may have a synergistic effect on enhancing AP-1 activity of the toxicity pathway of DON. These data indicate the high sensitivity and effectiveness of our biosensor system in the evaluation of the combined toxicity of ZEN, DON and their derivatives. In addition, this approach is suitable for an early warning method for the detection of ZEN and DON family mycotoxins contamination without higher-priced, conventional analytical chemistry methods. PMID:27498557

  14. Deoxynivalenol (DON) is toxic to human colonic, lung and monocytic cell lines, but does not increase the IgE response in a mouse model for allergy

    International Nuclear Information System (INIS)

    We examined whether the common crop mycotoxin deoxynivalenol (DON) from Fusarium species is toxic to human colonic (Caco-2), lung (A549) and monocytic (U937) cell lines. Moreover, since DON reportedly induces increased levels of Th2 cytokines and total IgE, and we have observed that mould extracts adjuvated allergy development in mice, possible adjuvant effect of DON on allergy was studied in a mouse model. For all the cells, exposure to DON for 24 h reduced cellular protein synthesis, proliferation and survival rate dose-dependently. In addition, production of IL-8 in the U937 cell line increased up to eight-fold at levels of DON just lower than the most toxic one, suggesting that IL-8 can be used as an additional index for cytotoxicity in mononuclear phagocytes. However, DON did not increase levels of allergen-specific IgE or IgG1 in the mouse model for allergy. These results suggest that DON, when inhaled or ingested, may have toxic effect on human alveolar macrophages and epithelial cells in lungs and colon, but does not increase the allergic response to allergens

  15. A chemist and biologist talk to each other about caged neurotransmitters

    OpenAIRE

    Ellis-Davies, Graham C. R.

    2013-01-01

    Caged compounds are small organic molecules that can be photoactivated with brief pulses of light. They are widely used to study a great variety of biological processes by physiologists, cell biologists and neuroscientists. Initially made and invented by biologists in the late 1970s, they are now made mostly by chemists, often without any dialogue with the end users, the biologists. The idea for this review is to stimulate interaction between the two communities to further the creative develo...

  16. Cell differentiation, secondary cell-wall formation and transformation of callus tissue of Pinus radiata D. Don.

    Science.gov (United States)

    Möller, Ralf; McDonald, Armando G; Walter, Christian; Harris, Philip J

    2003-09-01

    Tracheid and sclereid differentiation was induced in callus cultures of Pinus radiata D. Don by culturing on a basal medium containing activated charcoal but no phytohormones; sclereids differentiated in callus derived from xylem strips, but not in callus derived from hypocotyl segments. The tracheids differentiated in hypocotyl-derived callus had helical, scalariform, reticulated or pitted secondary cell-wall patterns, but those differentiated in xylem-derived callus had a reticulate or pitted pattern. The thickened tracheid and sclereid walls contained lignin as indicated by the red colour reaction given with phloroglucinol-HCl. The presence of lignin in the cell walls of differentiated callus was confirmed using pyrolysis gas chromatography-mass spectrometry by the detection of phenylpropanoid components derived from lignin. Lignin was also detected using solid-state (13)C cross-polarisation/magic-angle spinning nuclear magnetic resonance spectroscopy and quantified as thioglycolic acid lignin. Monosaccharide analyses of the cell walls isolated from differentiated and undifferentiated calli showed that the cell walls of the differentiated calli contained higher proportions of glucose and mannose, consistent with the presence of greater proportions of gluco- and/or galactogluco-mannans in the secondary cell walls of the differentiated cells. A protocol for the stable transformation of undifferentiated, xylem-derived cultures was successfully developed. Transgenic cell lines were established following Biolistic particle bombardment with a plasmid containing the coding region of the nptII gene and the coding region of the cad gene from P. radiata. Expression of the nptII gene in transgenic lines was confirmed by an NPTII-enzyme-linked immunosorbent assay. The overexpression of cad in the transgenic lines resulted in a down-regulation of cinnamyl alcohol dehydrogenase (EC 1.1.1.195) expression. PMID:12811558

  17. Tihhi Don

    Index Scriptorium Estoniae

    2006-01-01

    Fjodor Bondartshuk monteeris kokku tema isa Sergei poolt Itaalias 13 aastat tagasi pooleli jäänud Mihhail Sholohhovi romaani järgi tehtud teleseriaali "Vaikne Don", mida Venemaa televisiooni 1. Kanal näitab alates 7. novembrist

  18. Effects of deoxynivalenol (DON), zearalenone (ZEN), and related metabolites on equine peripheral blood mononuclear cells (PBMC) in vitro and background occurrence of these toxins in horses.

    Science.gov (United States)

    Schumann, Barbara; Winkler, Janine; Mickenautsch, Nicola; Warnken, Tobias; Dänicke, Sven

    2016-08-01

    Both deoxynivalenol (DON), zearalenone (ZEN), and their metabolites are known to modulate immune cells in various species whereby viability and proliferation are influenced. Such effects were rarely examined in horses. Therefore, one aim of the present study was to titrate the inhibitory concentrations of DON, 3-acetyl-DON (3AcDON), de-epoxy-DON (DOM-1), ZEN, and α- and β-zearalenol (ZEL) at which viability and proliferation of equine PBMC were reduced by 50 % (IC50) and 10 % (IC10) in vitro. For evaluation of practical relevance of the in vitro findings, a further aim was to screen horses for the background occurrence of DON, ZEN, and their metabolites in systemic circulation and to relate toxin residues both to the inhibitory toxin concentrations and to hematological and clinical-chemical characteristics.The IC50 (μM) for DON, 3AcDON, β-ZEL, α-ZEL, and ZEN were determined at 3.09, 25.90, 75.44, 97.44, and 98.15 in unstimulated cells, respectively, while in proliferating cells, the corresponding IC50 values were 0.73, 6.89, 45.16, 75.96, and 82.51. Neither viability nor proliferation was influenced by DOM-1 up to a concentration of 100 μM.The in vivo screening (N = 49) revealed the occurrence of ZEN (N = 24), α-ZEL (N = 3), β-ZEL (N = 37), DON, and DOM-1 (N = 2). The detected concentrations were much lower than the corresponding IC50 while the IC10 of DON and β-ZEL for proliferating PBMC corresponded to approximately 26 and 35 ng/mL which might be relevant when contaminated diets are fed.Clinical-chemical and hematological traits were not related to mycotoxin residue levels excepting blood urea nitrogen which was positively correlated to the sum of β-ZEL, α-ZEL, and ZEN concentration. Whether this reflects simply the feeding history of the horses or renal failures giving rise to a prolonged half-life of the toxins needs to be clarified further. PMID:27255919

  19. A Biologist Looks at Cognitive Artificial Intelligence

    OpenAIRE

    Purves, William K.

    1985-01-01

    Although cognitive AI is not generally viewed as being "scientific" in the same, strong sense as is physics, it shares a number of the properties of the natural sciences, especially biology. Certain of special themes of biology, notably the principles of historicity and of structure-function relations, are applicable in AI research. From a biologist's viewpoint, certain principles of cognitive AI research emerge.

  20. Computing Workflows for Biologists: A Roadmap

    OpenAIRE

    Shade, Ashley; Teal, Tracy K.

    2015-01-01

    Extremely large datasets have become routine in biology. However, performing a computational analysis of a large dataset can be overwhelming, especially for novices. Here, we present a step-by-step guide to computing workflows with the biologist end-user in mind. Starting from a foundation of sound data management practices, we make specific recommendations on how to approach and perform computational analyses of large datasets, with a view to enabling sound, reproducible biological research.

  1. Proton/Phosphate Stoichiometry in Uptake of Inorganic Phosphate by Cultured Cells of Catharanthus roseus (L.) G. Don

    Science.gov (United States)

    Sakano, Katsuhiro

    1990-01-01

    Upon absorption of phosphate, cultured cells of Catharanthus roseus (L.) G. Don caused a rapid alkalinization of the medium in which they were suspended. The alkalinization continued until the added phosphate was completely exhausted from the medium, at which time the pH of the medium started to drop sharply toward the original pH value. Phosphate exposure caused the pH of the medium to increase from pH 3.5 to values as high as 5.8, while the rate of phosphate uptake was constant throughout (10-17 micromoles per hour per gram fresh weight). This indicates that no apparent pH optimum exists for the phosphate uptake by the cultured cells. The amount of protons cotransported with phosphate was calculated from the observed pH change up to the maximum alkalinization and the titration curve of the cell suspension. Proton/phosphate transport stoichiometry ranged from less than unity to 4 according to the amount of phosphate applied. At low phosphate doses, the stoichiometries were close to 4, while at high phosphate doses, smaller stoichiometries were observed. This suggests that, at high phosphate doses, activation of the proton pump is induced by the longer lasting proton influx acidifying the cytoplasm. The increased H+ efflux due to the proton pump could partially compensate protons taken up via the proton-phosphate cotransport system. Thus, the H+/H2PO4− stoichiometry of the cotransport is most likely to be 4. PMID:16667491

  2. Entropy for biologists an introduction to thermodynamics

    CERN Document Server

    Morowitz, Harold J

    2013-01-01

    Entropy for Biologists: An Introduction to Thermodynamics is an introductory book for people in the life sciences who wish to master the concepts of thermal physics without being forced to a degree and rate of symbol manipulation which is foreign to their patterns of thought. The book opens with a chapter on temperature, followed by separate chapters that discuss the concepts of energy, kinetic theory, total energy, the second law of thermodynamics, entropy, and probability and information theory. Subsequent chapters deal with statistical mechanics and its relation to thermodynamics, free-ener

  3. Lessons from a great developmental biologist.

    Science.gov (United States)

    De Robertis, Edward M

    2014-07-01

    The announcement that Sir John Gurdon had been awarded the 2012 Nobel Prize for Medicine or Physiology was received with great joy by developmental biologists. It was a very special occasion because of his total dedication to science and turning the Golden Rule of western civilization - love your neighbor as yourself - into a reality in our field. This essay attempts to explain how John became such a great scientific benefactor, and to review some of his discoveries that are less well known than the nuclear transplantation experiments. A few personal anecdotes are also included to illustrate the profound goodness of this unique man of science. PMID:25455202

  4. Lessons from a Great Developmental Biologist

    Science.gov (United States)

    De Robertis, Edward M.

    2014-01-01

    The announcement that Sir John Gurdon had been awarded the 2012 Nobel Prize for Medicine or Physiology was received with great joy by developmental biologists. It was a very special occasion because of his total dedication to science and turning the Golden Rule of western civilization – love your neighbor as yourself – into a reality in our field. This essay attempts to explain how John became such a great scientific benefactor, and to review some of his discoveries that are less well known than the nuclear transplantation experiments. A few personal anecdotes are also included to illustrate the profound goodness of this unique man of science. PMID:25455202

  5. Stem cell therapy for white matter disorders: don't forget the microenvironment!

    Science.gov (United States)

    Dooves, Stephanie; van der Knaap, Marjo S; Heine, Vivi M

    2016-07-01

    White matter disorders (WMDs) are a major source of handicap at all ages. They often lead to progressive neurological dysfunction and early death. Although causes are highly diverse, WMDs share the property that glia (astrocytes and oligodendrocytes) are among the cells primarily affected, and that myelin is either not formed or lost. Many WMDs might benefit from cell replacement therapies. Successful preclinical studies in rodent models have already led to the first clinical trials in humans using glial or oligodendrocyte progenitor cells aiming at (re)myelination. However, myelin is usually not the only affected structure. Neurons, microglia, and astrocytes are often also affected and are all important partners in creating the right conditions for proper white matter repair. Composition of the extracellular environment is another factor to be considered. Cell transplantation therapies might therefore require inclusion of non-oligodendroglial cell types and target more than only myelin repair. WMD patients would likely benefit from multimodal therapy approaches involving stem cell transplantation and microenvironment-targeting strategies to alter the local environment to a more favorable state for cell replacement. Furthermore most proof-of-concept studies have been performed with human cells in rodent disease models. Since human glial cells show a larger regenerative capacity than their mouse counterparts in the host mouse brain, microenvironmental factors affecting white matter recovery might be overlooked in rodent studies. We would like to stress that cell replacement therapy is a highly promising therapeutic option for WMDs, but a receptive microenvironment is crucial. PMID:27000179

  6. Cell Phones in the Classroom: Don't Put Them Away Just Yet!

    Science.gov (United States)

    Webb, Carolyn Lorraine

    2013-01-01

    As the number of students with cell phones has steadily increased over the past decade, these technological advances have caused trepidation among educators over behavioral issues, from off-task activities to cheating (Prensky, 2005). On the other hand, mobile learning, as well as some tools to use with cell phones, are easy to set up, easy to…

  7. Jean Rostand, an anti-nuclear biologist

    International Nuclear Information System (INIS)

    In the 70's, some scientists, like the French biologist Jean Rostand, were already militating in favour of solar-derived energy sources. One of his favored domain of thoughts was the one of relations between science, ethics and politics. Jean Rostand did not limit his writing work to biology but looked into the moral and political impacts of discoveries in the other scientific domains as well. From the 50's and during several years onward, he got strongly and deeply involved in a radical fight against the 'French atomic bomb'. He particularly stressed on the fact that 'the later we react, the harder will be the energy transition'. This book brings together his texts and conference talks against nuclear weapons and civil nuclear energy. From this compilation of documents, the author notices that, thirty five years later, these documents remain as topical as in the past, and that no energy reconversion has been undertaken by none of the successive French governments who remained under the double control of the petroleum and nuclear industry lobbies. This policy has led to limit the development of energy conservation and renewable energy sources and to increase the lateness of France in this domain with respect to other European countries

  8. The Don Bosco Story.

    Science.gov (United States)

    Galza, Ma. Lourdes LL.

    1985-01-01

    Describes the work of the Don Bosco Educational Association of the Philippines, which operates trade schools for boys. The article focuses on the Don Bosco College's engineering technology and general engineering science programs and the development of Industrial Technician Centers. Other programs operated by the Salesians are also described. (CT)

  9. Attributes of Biologists in Two-Year Colleges

    Science.gov (United States)

    Creager, Joan G.; Ehrle, Elwood B.

    1971-01-01

    Presents results of a questionnaire survey of sex, age, qualifications, work experience, teaching load, research and publication activities, professional society membership, and salaries of biologists employed in junior colleges in the United States. (AL)

  10. Lack of Control in Inorganic Phosphate Uptake by Catharanthus roseus (L.) G. Don Cells (Cytoplasmic Inorganic Phosphate Homeostasis Depends on the Tonoplast Inorganic Phosphate Transport System?).

    Science.gov (United States)

    Sakano, K.; Yazaki, Y.; Okihara, K.; Mimura, T.; Kiyota, S.

    1995-01-01

    Inorganic phosphate (Pi) uptake by Catharanthus roseus (L.) G. Don cells was studied in relation to its apparent uncontrolled uptake using 31P-nuclear magnetic resonance spectroscopy. Kinetics of Pi uptake by the cells indicated that apparent Km and Vm were about 7 [mu]M and 20 [mu]mol g-1 fresh weight h-1, respectively. Pi uptake in Murashige-Skoog medium under different Pi concentrations and different initial cell densities followed basically the same kinetics. When supplied with abundant Pi, cells absorbed Pi at a constant rate (Vm) for the first hours and accumulated it in the vacuole. As the endogenous pool expanded, the rate of Pi uptake gradually decreased to nil. Maximum Pi accumulation was 100 to 120 [mu]mol g-1 fresh weight if cell swelling during Pi uptake (about 2-fold in cell volume) was not considered. Results indicated that (a) the rate of Pi uptake by Catharanthus cells was independent of initial cell density and was constant over a wide range of Pi concentrations (2 mM to about 10 [mu]M) unless the cells were preloaded with excess Pi, and (b) there was no apparent feedback control over the Pi uptake process in the plasma membrane to avoid Pi toxicity. The importance of the tonoplast Pi transport system in cytoplasmic Pi homeostasis is discussed. PMID:12228474

  11. Flavonoids isolated from Lespedeza cuneata G. Don and their inhibitory effects on nitric oxide production in lipopolysaccharide-stimulated BV - 2 microglia cells

    Directory of Open Access Journals (Sweden)

    Guijae Yoo

    2015-01-01

    Full Text Available Background: Lespedeza cuneata (Dum. Cours. G. Don, a perennial legume native to Eastern Asia, has been used therapeutically in traditional Asian medicine to protect the function of liver, kidneys and lungs. However, its effect on inflammatory nitric oxide (NO production and the active constituents have not yet been explored. Objective: In this study, we investigated the phytochemical constituents of L. cuneata and evaluated their effect on NO production using lipopolysaccharide (LPS stimulated BV2 cells. Materials and Methods: The 80% methanol extract of the aerial part of L. cuneata were used for the isolation of flavonoids. The isolated compounds were elucidated by various spectroscopic methods including nuclear magnetic resonance and mass spectrometry spectrometry. To evaluate the effect on inflammatory NO production, LPS stimulated murine microglia BV 2 cells were used as a screening system. Results: Nine flavonoids were isolated from the aerial parts of L. cuneata. Among the isolated flavonoids, compounds 4, 5, 7 and 9 are reported from the genus Lespedeza for the first time. Moreover, compounds 1 and 6 showed significant inhibitory effects on NO production in LPS stimulated BV2 cells without cell toxicity. Conclusion: In this study, nine flavonoids were isolated from L. cuneata. Among the compounds, only 1 and 6, which have free hydroxyl groups at both C3 and C7 showed significant inhibitory activity on NO production in LPS stimulated BV2 cells. These results suggested L. cuneata and its flavonoid constituents as possible candidate for the treatment of various inflammatory diseases.

  12. Don Quixote. [Lesson Plan].

    Science.gov (United States)

    Rooks, Kristen

    Based on Miguel de Cervantes' novel "Don Quixote," this lesson plan presents activities designed to help students understand that Quixote's misperceptions are understandable; writers often describe one object to sound as if it were something else; and metaphors help readers see with new eyes. The main activity of the lesson involves students…

  13. The Metabolic Fate of Deoxynivalenol and Its Acetylated Derivatives in a Wheat Suspension Culture: Identification and Detection of DON-15-O-Glucoside, 15-Acetyl-DON-3-O-Glucoside and 15-Acetyl-DON-3-Sulfate

    Directory of Open Access Journals (Sweden)

    Clemens Schmeitzl

    2015-08-01

    Full Text Available Deoxynivalenol (DON is a protein synthesis inhibitor produced by the Fusarium species, which frequently contaminates grains used for human or animal consumption. We treated a wheat suspension culture with DON or one of its acetylated derivatives, 3-acetyl-DON (3-ADON, 15-acetyl-DON (15-ADON and 3,15-diacetyl-DON (3,15-diADON, and monitored the metabolization over a course of 96 h. Supernatant and cell extract samples were analyzed using a tailored LC-MS/MS method for the quantification of DON metabolites. We report the formation of tentatively identified DON-15-O-β-D-glucoside (D15G and of 15-acetyl-DON-3-sulfate (15-ADON3S as novel deoxynivalenol metabolites in wheat. Furthermore, we found that the recently identified 15-acetyl-DON-3-O-β-D-glucoside (15-ADON3G is the major metabolite produced after 15-ADON challenge. 3-ADON treatment led to a higher intracellular content of toxic metabolites after six hours compared to all other treatments. 3-ADON was exclusively metabolized into DON before phase II reactions occurred. In contrast, we found that 15-ADON was directly converted into 15-ADON3G and 15-ADON3S in addition to metabolization into deoxynivalenol-3-O-β-D-glucoside (D3G. This study highlights significant differences in the metabolization of DON and its acetylated derivatives.

  14. Reinventing College Physics for Biologists: Explicating an epistemological curriculum

    OpenAIRE

    Edward F. Redish; Hammer, David

    2008-01-01

    The University of Maryland Physics Education Research Group (UMd-PERG) carried out a five-year research project to rethink, observe, and reform introductory algebra-based (college) physics. This class is one of the Maryland Physics Department's large service courses, serving primarily life-science majors. After consultation with biologists, we re-focused the class on helping the students learn to think scientifically -- to build coherence, think in terms of mechanism, and to follow the implic...

  15. The descent of language. A conversation between two jobless biologists

    OpenAIRE

    Giovanni D'Angelo; Parashuraman Seetharaman

    2013-01-01

    Seen from the perspective of a biologist, the issue of the origin of language contains an inherent ambiguity. On the one hand, one might think to explore the cognitive features or even the anatomical structures related to communication through the peculiar medium called verbal language, a characteristic property emergent among the Homo sapiens. On the other hand, if one decides to restrict oneself to the formal definition of language as a system of signs for encoding information, then, the...

  16. Production Pattern of Ajmalicine in Catharanthus roseus (L.) G. Don. Cell Aggregates Culture in the Airlift Bioreactor

    OpenAIRE

    RIZKITA RACHMI ESYANTI; AIDA MUSPIAH

    2006-01-01

    A research has been conducted to optimize the rate of aeration and initial weight of cell aggregates in the production of ajmalicine in Catharanthus roseus cell culture in airlift bioreactor. Catharanthus roseus culture were grown in Zenk medium with the addition of 2.50 x 10-6 M naphthalene acetic acid (NAA) and 10-5 M benzyl amino purine (BAP). Cell aggregates were sub-cultured two times before transferring 20 and 30 g/fw of cell aggregates into bioreactor, respectively, and aerated with th...

  17. "Owl's eye" cells in a cervical smear of a transplant recipient: don't forget to inform the referring physician.

    NARCIS (Netherlands)

    Oei, A.L.M.; Salet-van de Pol, M.R.J. van de; Borst, S.M.; Berg, A.P. van den; Grefte, J.M.M.

    2007-01-01

    Cytomegalovirus (CMV) infected cells in cervical smears are a rare finding but may have severe consequences. We describe the presence of characteristic "owl eye" cells in a conventional cervical smear. Medical history revealed a liver transplantation from a CMV seropositive donor 1 yr earlier. The p

  18. 'Owl's eye' cells in a cervical smear of a transplant recipient : Don't forget to inform the referring physician

    NARCIS (Netherlands)

    Oei, A. L. M.; Salet-van de Pol, M. R. J.; Borst, S. M.; van den Berg, A. P.; Grefte, J. M. M.

    2007-01-01

    Cytomegalovirus (CMV) infected cells in cervical smears are a rare finding but may have severe consequences. We describe the presence of characteristic 'owl eye' cells in a conventional cervical smear. Medical history revealed a liver transplantation from a CMV seropositive donor 1 yr earlier. The p

  19. Production Pattern of Ajmalicine in Catharanthus roseus (L. G. Don. Cell Aggregates Culture in the Airlift Bioreactor

    Directory of Open Access Journals (Sweden)

    RIZKITA RACHMI ESYANTI

    2006-12-01

    Full Text Available A research has been conducted to optimize the rate of aeration and initial weight of cell aggregates in the production of ajmalicine in Catharanthus roseus cell culture in airlift bioreactor. Catharanthus roseus culture were grown in Zenk medium with the addition of 2.50 x 10-6 M naphthalene acetic acid (NAA and 10-5 M benzyl amino purine (BAP. Cell aggregates were sub-cultured two times before transferring 20 and 30 g/fw of cell aggregates into bioreactor, respectively, and aerated with the rate of 0.25 l min-1 and 0.34 l min-1, respectively. The pattern of ajmalicine production in bioreactor were observed in every three days within 24 days. Qualitative and quantitative analysis were conducted using HPLC connected to Cromatopac CL-7A Plus. The results showed that the cell aggregates and medium contain ajmalicine. The highest concentration was obtained in combination of 30 g/fw and 0.34 l min-1 aeration compare to 20 g/fw - 0.25 l min-1, 20 g/fw - 0.34 l min-1, as well as 30 g/fw – 0.25 l min-1. The highest ajmalicine content in cell aggregates was obtained on the 12 days (79.23 µg g-1 whilst in medium was obtained in the 18th days (981.15 µg l-1.

  20. The UCSC Genome Browser: What Every Molecular Biologist Should Know

    Science.gov (United States)

    Mangan, Mary E.; Williams, Jennifer M.; Kuhn, Robert M.; Lathe, Warren C.

    2016-01-01

    Electronic data resources can enable molecular biologists to query and display many useful features that make benchwork more efficient and drive new discoveries. The UCSC Genome Browser provides a wealth of data and tools that advance one’s understanding of genomic context for many species, enable detailed understanding of data, and provide the ability to interrogate regions of interest. Researchers can also supplement the standard display with their own data to query and share with others. Effective use of these resources has become crucial to biological research today, and this unit describes some practical applications of the UCSC Genome Browser. PMID:19816931

  1. Mimesis in Don Quixote

    Directory of Open Access Journals (Sweden)

    Giselle von der Walde

    2006-04-01

    Full Text Available Resumen:Frente a argumentos tomados de las poéticas neoaristotélicas que esgrime el canónigo para condenar los libros de caballerías, don Quijote pretende mostrar con su propio ejemplo, que ese tipo de lecturas no llevan a la locura ni al abandono de sí mismo, sino que, por el contrario, sacan lo mejor de la naturaleza de un individuo y lo hacen mejor persona. Este es el único tipo de imitación que Platón acepta en la República, pues más que un remedo o una suplantación, implica emulación. Este escrito se propone mirar, a partir del diálogo con el canónigo de Toledo (I, 49-50, cómo en sus conductas miméticas desde el comienzo de la obra, don Quijote entiende la imitación como emulación; en consecuencia se intenta demostrar que el efecto de la literatura en el caballero manchego parece ser el tipo de mimesis que Platón acepta en el libro III de la República.Abstract:Don Quijote demonstrates with the example of his own life the error made by the Canon of Toledo when the latter uses the arguments of the neo-Aristotelian poets to condemn the novels of chivalric romance. Quijote tries to show that reading the chivalric romances leads to neither madness nor the abandonment of the self, but, on the contrary, brings out the best in a person’s nature, and makes one a better person. This is the only type of imitation that Plato accepts in the Republic, because, unlike the mere copying of a person, or the substitution of one person for another, it implies a type of emulation. Starting with the dialogue of the Canon of Toledo (I, 49-50, this paper shows how, in his mimetic behavior, Don Quijote understands imitation as emulation. As a result, the paper shows that the effect of literature on the gentleman from La Mancha is the type of mimesis of which Plato approves in book III of the Republic.

  2. "We don't wear it on our sleeve": Sickle cell disease and the (in)visible body in parts.

    Science.gov (United States)

    Ciribassi, Rebekah M; Patil, Crystal L

    2016-01-01

    This paper approaches the lived experiences of patients with a genetically inherited chronic disease, sickle cell disease (SCD), through the lens of (in)visibility. SCD has been referred to as an "invisible" disease for a variety of interrelated reasons, including the difficulty of objectively measuring its characteristic symptoms, the lack of popular or specialist attention, and its characterization as a "black" disease. By mobilizing "invisibility" as a way of probing the day-to-day reinforcements of marginality, this article delves into how structural forces are experienced, interpreted, and negotiated by individual actors. To this end, we present ethnographic data collected from November 2009 until November 2013 with SCD patients and healthcare workers in Chicago. These data emphasize that rendering (in)visible is not a totalizing act, but rather meaningfully breaks the body into differentially visible and ideology-laden parts. More broadly, this indicates the need to rigorously question sources and effects of authority in biomedicine. PMID:26692094

  3. A Biologist's Field Guide to Multiplexed Quantitative Proteomics.

    Science.gov (United States)

    Bakalarski, Corey E; Kirkpatrick, Donald S

    2016-05-01

    High-throughput genomic and proteomic studies have generated near-comprehensive catalogs of biological constituents within many model systems. Nevertheless, static catalogs are often insufficient to fully describe the dynamic processes that drive biology. Quantitative proteomic techniques address this need by providing insight into closely related biological states such as the stages of a therapeutic response or cellular differentiation. The maturation of quantitative proteomics in recent years has brought about a variety of technologies, each with their own strengths and weaknesses. It can be difficult for those unfamiliar with this evolving landscape to match the experiment at hand with the best tool for the job. Here, we outline quantitative methods for proteomic mass spectrometry and discuss their benefits and weaknesses from the perspective of the biologist aiming to generate meaningful data and address mechanistic questions. PMID:26873251

  4. Reinventing College Physics for Biologists: Explicating an epistemological curriculum

    CERN Document Server

    Redish, Edward F

    2008-01-01

    The University of Maryland Physics Education Research Group (UMd-PERG) carried out a five-year research project to rethink, observe, and reform introductory algebra-based (college) physics. This class is one of the Maryland Physics Department's large service courses, serving primarily life-science majors. After consultation with biologists, we re-focused the class on helping the students learn to think scientifically -- to build coherence, think in terms of mechanism, and to follow the implications of assumptions. We designed the course to tap into students' productive conceptual and epistemological resources, based on a theoretical framework from research on learning. The reformed class retains its traditional structure in terms of time and instructional personnel, but we modified existing best-practices curricular materials, including Peer Instruction, Interactive Lecture Demonstrations, and Tutorials. We provided class-controlled spaces for student collaboration, which allowed us to observe and record stud...

  5. Don't panic

    Science.gov (United States)

    Harris, Margaret

    2009-06-01

    "Don't panic" is a simple piece of advice, one that usually applies equally well to job-hunting, avoiding pandemic flu or, like the hapless Arthur Dent in the Hitchhiker's Guide to the Galaxy stories, wandering the universe in search of a decent cup of tea. Yet some statistics emerging from the current job market are undeniably alarming. Postings on the recruitment website Milkround.com, which advertises jobs and internships for recent graduates, are down by about 20% compared with this time last year, while a survey earlier this year by High Fliers, a Londonbased research firm, found that top UK employers plan to recruit 17% fewer graduates this year than in 2008. In the same study, half of the 1017 final-year students surveyed believed they would have to take "any graduate job" they were offered, regardless of their interest in the company, and a whopping 91% thought competition for vacancies would be tougher than last year.

  6. The Don'ts and Don'ts of Teaching

    Science.gov (United States)

    Rubinstein, Gary

    2012-01-01

    Gary Rubinstein doesn't buy the adage that rules--for students or for teachers--must be stated positively, without don'ts or nots. So he lists 10 rules for things teachers must not do in their first years. Tops on his list is "Don't try to teach too much in one day," which Rubinstein says is a common mis-step because teacher preparation programs,…

  7. Flavonoids isolated from Lespedeza cuneata G. Don and their inhibitory effects on nitric oxide production in lipopolysaccharide-stimulated BV-2 microglia cells

    OpenAIRE

    Guijae Yoo; Seon Ju Park; Taek Hwan Lee; Heejung Yang; Yoon-su Baek; Nanyoung Kim; Yoon Jae Kim; Seung Hyun Kim

    2015-01-01

    Background: Lespedeza cuneata (Dum. Cours.) G. Don, a perennial legume native to Eastern Asia, has been used therapeutically in traditional Asian medicine to protect the function of liver, kidneys and lungs. However, its effect on inflammatory nitric oxide (NO) production and the active constituents have not yet been explored. Objective: In this study, we investigated the phytochemical constituents of L. cuneata and evaluated their effect on NO production using lipopolysaccharide (LPS) stimul...

  8. "Tihhi Don" - za i protiv

    Index Scriptorium Estoniae

    2006-01-01

    Võrdluses on Mihhail Sholohhovi raamatu "Vaikne Don" kaks ekraniseeringut : Sergei Gerassimovi (1975) ja Sergei Bondartshuki (1991) oma. Võrreldakse peaosatäitjaid, meenutatakse huvitavaid fakte filmivõtetelt. Katkend Elina Bõstritskaja mälestusteraamatust

  9. Vaikne Don : [luuletused] / Mart Kangur

    Index Scriptorium Estoniae

    Kangur, Mart

    2004-01-01

    Sisu: Vaikne Don ; "Imelik, kui kerge on hakata armastama neid ..." ; "Laud otsekui tool ..." ; "Raamat on monument ..." ; "Nägu on liiga tühi ..." ; "Mida teeb Buddha pärast virgumist? Peseb hambaid ..."

  10. Pregnant? Don't Smoke!

    Science.gov (United States)

    ... What's this? Submit Button Past Emails CDC Features Pregnant? Don't Smoke! Language: English Español (Spanish) Recommend ... problems. The Good News Quitting smoking before getting pregnant is best. But for women who are already ...

  11. Effect of deoxynivalenol (DON) mycotoxin on in vivo and in vitro porcine circovirus type 2 infections.

    Science.gov (United States)

    Savard, Christian; Provost, Chantale; Alvarez, Fernando; Pinilla, Vicente; Music, Nedzad; Jacques, Mario; Gagnon, Carl A; Chorfi, Younes

    2015-04-17

    Deoxynivalenol (DON) is a mycotoxin produced by Fusarium spp and is a common contaminant of grains in North America. Among farm animals, swine are the most susceptible to DON because it markedly reduces feed intake and decreases weight gain. Porcine circovirus type 2 (PCV2) is the main causative agent of several syndromes in weaning piglets collectively known as porcine circovirus-associated disease (PCVAD). The objectives of this study were to investigate the impact of DON on PCV2 replication in NPTr permissive cell line, and to determine eventual potentiating effects of DON on PCV2 infection in pigs. Noninfected and infected cells with PCV2 were treated with increasing concentrations of DON (0, 70, 140, 280, 560, 1200 ng/mL) and cell survival and virus titer were evaluated 72 h postinfection. Thirty commercial piglets were randomly divided into 3 experimental groups of 10 animals based on DON content of served diets (0, 2.5 and 3.5 mg/kg DON). All groups were further divided into subgroups of 6 pigs and were inoculated with PCV2b virus. The remaining pigs (control) were sham-inoculated with PBS. In vitro results showed that low concentrations of DON could potentially increase PCV2 replication depending on virus genotype. In vivo results showed that even though viremia and lung viral load tend to be higher in animal ingesting DON contaminated diet at 2.5 mg/kg, DON had no significant effect on clinical manifestation of PCVAD in PCV2b infected animals. DON has neither in vitro nor in vivo clear potentiating effects in the development of porcine circovirus infection despite slight increases in viral replication. PMID:25717015

  12. Odgajajmo s don Boscovim srcem

    OpenAIRE

    Chávez Villanueva, Pascual

    2008-01-01

    Don Bosco svojim životnim primjerom kao odgajatelj pokazuje kako je uspješan odgoj ujedno i kvalitetan odgoj. On je svugdje i na svakom mjestu bio odgajatelj. Za dobar odgoj potrebni su: dobar odgajatelj, dobro odgojno ozračje, dobar odgojni program i dobro odgojno iskustvo. Don Boscovo mjerilo u odgoju je razvoj pozitivnog poticaja odnosno pozitivne želje koji postoje u svakom mladom čovjeku. To uključuje i povezanost s kulturnom baštinom i ponudu iskustva vjere. Odgajanika se uključuje u dr...

  13. Rejoice in the hubris: useful things biologists could do for physicists

    Science.gov (United States)

    Austin, Robert H.

    2014-10-01

    Political correctness urges us to state how wonderful it is to work with biologists and how, just as the lion will someday lie down with the lamb, so will interdisciplinary work, where biologists and physicists are mixed together in light, airy buildings designed to force socialization, give rise to wonderful new science. But it has been said that the only drive in human nature stronger than the sex drive is the drive to censor and suppress, and so I claim that it is OK for physicists and biologists to maintain a wary distance from each other, so that neither one censors or suppresses the wild ideas of the other.

  14. Career Choices by Biologists: A Case Study of Destinations of Graduates in Marine Biology and Zoology, 1986-1998.

    Science.gov (United States)

    Gill, Margaret E.; Golding, David W.

    2001-01-01

    Reports on a study tracking marine biologists' and zoologists' employment status between the years of 1986 and 1998. Suggests that despite the increase in university class sizes, able and well-trained biologists enjoy good career prospects. (Author/YDS)

  15. Don Ross, Philosophy of Economics

    OpenAIRE

    Claveau, François

    2016-01-01

    A possible misunderstanding must be addressed at the outset. In this book, Don Ross offers his philosophy of economics, not a survey of an area of contemporary philosophy that is conventionally referred to as philosophy of economics. Ross is explicit about that (Author’s preface, x). In consequence, the book is an outlier as a title in the series Palgrave Philosophy Today, a series “meant to help all philosophers, established and aspiring, to understand, appreciate and engage with the intrica...

  16. Regulation of IGF-I production and proliferation of human leiomyomal smooth muscle cells by Scutellaria barbata D. Don in vitro: isolation of flavonoids of apigenin and luteolin as acting compounds

    International Nuclear Information System (INIS)

    Scutellaria barbata D. Don (Lamiaceae) (SB) is a perennial herb, which is natively distributed throughout Korea and southern China. This herb is known in traditional Chinese Medicine as Ban-Zhi-Lian and traditional Korean medicine as Banjiryun, respectively. SB has been used as an anti-inflammatory and antitumor agent. We aimed to determine the expression of growth factor molecules for growth inhibition after treatment of SB in two different human myometrial smooth muscle cell (SMC)s and leiomyomal SMCs. Water-soluble ingredients of SB, myometrial SMCs, and the leiomyomal cell lines were used in vitro. SB significantly reduced cell numbers in culture and arrested cell proliferation, and also induced apoptosis, indicating that the presence of an intact apoptotic pathway was demonstrated in these cells by SB. Uterine leiomyoma is the most common benign smooth muscle cell tumor of the myometrium. The expression of insulin-like growth factor-I (IGF-I) was measured at the mRNA and protein level in myometrium and leiomyomal cells with and without treatment with a water extract of SB for 3 days. IGF-I mRNA expression was significantly higher in leiomyomal cells than in myometrium cells. The IGF-I protein was more abundant in leiomyomal cells than in myometrium. When SB was treated to the cells, the IGF-I protein concentrations in myometrial and leiomyomal cells from the SB-treated cells were similar. The results indicated that IGF-I expression is probably associated with a proliferation of leiomyomal cells than myometrium. However, SB down-regulated the IGF-I expression where IGF-I contributes to the selective growth of the leiomyoma. Therefore, growth modulation of LMs by SB occurs via mechanisms dependent of apoptosis. The raw materials were extracted and subjected to functional isolation for the active molecules in the present assay systems. The five flavonoids were isolated and the chemical structures of resveratrol, baicalin, berberine, apigenin, and luteolin were

  17. Stewart, Don oral history interview

    OpenAIRE

    Interviewee: Stewart, Don; Interviewer: Manlove, Courtney; Principal Investigator: Hall, Peter V

    2014-01-01

    Don Stewart was born at St Mary’s Hospital in New Westminster in 1944, and had six siblings. He was the son of a mill worker at MacMillan Bloedel, who was also a caretaker at Spring Ranch located in the Connaught Heights area of New Westminster. It was called Spring Ranch because it had its own “artesian well” or spring that generated two small pools that Stewart and his friends would swim in during the summer. The family lived on the ranch between 1944 and 1952, and Stewart’s descriptions of...

  18. [Don Quijote, a lucid mad].

    Science.gov (United States)

    Alonso Fernández, Francisco

    2004-01-01

    The case of a 50-year-old hidalgo who believed to be transformed into a knight-errant named Don Quijote is a megalomaniac systematized delusion of transformation of the self, a delusion of metamorphosis in reference to the patient's own identity. The outward projection of this syndrome produces some delusional misidentifications of others, things and animals and include elements of a persecutory delusion which increase the grandiosity of the self. At the same time the hidalgo was maniac with a pathway of bipolar disorder. The phenomenon of donquijotismo is described as the defence of wasted causes and Sancho Panza as an illiterate Sócrates. PMID:15997586

  19. Don DeLillos Bilder

    Directory of Open Access Journals (Sweden)

    Diesner, Fabian

    2005-01-01

    Full Text Available The American author Don DeLillo is a master of creating powerful images, which want to be discovered, read and interpreted. To have a closer look at his novel Mao II from the Visual Studies' point of view reveals the complexity of literary images and the diverse references that are evoked. DeLillo plays with the reader's memory of perception, asks fundamental questions about our society ruled by mass media and simply delights with a picturesque style of narration. The central aspects in his novel are photography in general, the visual arts especially by Andy Warhol and the visual representation of mass media, particularly television.

  20. Microbial proteomics: a mass spectrometry primer for biologists

    Directory of Open Access Journals (Sweden)

    Graham Ciaren

    2007-08-01

    Full Text Available Abstract It is now more than 10 years since the publication of the first microbial genome sequence and science is now moving towards a post genomic era with transcriptomics and proteomics offering insights into cellular processes and function. The ability to assess the entire protein network of a cell at a given spatial or temporal point will have a profound effect upon microbial science as the function of proteins is inextricably linked to phenotype. Whilst such a situation is still beyond current technologies rapid advances in mass spectrometry, bioinformatics and protein separation technologies have produced a step change in our current proteomic capabilities. Subsequently a small, but steadily growing, number of groups are taking advantage of this cutting edge technology to discover more about the physiology and metabolism of microorganisms. From this research it will be possible to move towards a systems biology understanding of a microorganism. Where upon researchers can build a comprehensive cellular map for each microorganism that links an accurately annotated genome sequence to gene expression data, at a transcriptomic and proteomic level. In order for microbiologists to embrace the potential that proteomics offers, an understanding of a variety of analytical tools is required. The aim of this review is to provide a basic overview of mass spectrometry (MS and its application to protein identification. In addition we will describe how the protein complexity of microbial samples can be reduced by gel-based and gel-free methodologies prior to analysis by MS. Finally in order to illustrate the power of microbial proteomics a case study of its current application within the Bacilliaceae is given together with a description of the emerging discipline of metaproteomics.

  1. An Analysis of Journal Use by Structural Biologists with Applications for Journal Collection Development Decisions.

    Science.gov (United States)

    Lascar, Claudia; Mendelsohn, Loren D.

    2001-01-01

    Using bibliometric methodologies, this article analyzes the publication and citation patterns of a sample group of structural biologists from multiple institutions. Results were consistent with several models for journal selection. These models were used to compile a short list of specialized titles supporting structural biology. Results have…

  2. EVALUATION OF SOME ANTIOXIDANTS’ EFFECTS IN KIDNEY HISTOLOGICAL STRUCTURE OF WEANED PIGS, INTOXICATED WITH DEOXYNIVALENOL (DON

    Directory of Open Access Journals (Sweden)

    GABI DUMITRESCU

    2013-12-01

    Full Text Available Deoxynivalenol is a mycotoxin produced by fungi of the Fusarium genera, which are abundant in various cereal crops and processed grains. In order to protect cell structure within the tissues studied, we tried various experimental variants by incorporating some selenium and boron sources into the combined fodder, and also in mixture with DON with different concentrations. To determine the morphophysiological changes induced by the mycotoxin DON upon kidney histological structure, and also the antioxidants’ effects, we took samples from 9 piglets belonging to three groups: group 1 (fed with fodder added with DON, group 2 (fed with fodder added with selenium and group 3 (fed with fodder added with selenium and DON. At renal parenchyma level, DON determines a series of changes in the renal corpuscles and also in the uriniferous tubes. These changes are represented by glomerular atrophies and nephrocyte epithelial dystrophies. The epithelial dystrophic processes occur in the renal medullar, too, where numerous ectasied capillaries and hemorrhagic areas are present. In the case of groups 2 and 3 consisted of piglets fed with fodder added with selenium, respectively fodder added with DON and selenium, the renal vascular network becomes hypertrophic associated with leukocyte infiltrative processes.

  3. Cytological changes in meristematic cells of Allium cepa L. root tip treated with extracts from callus of Catharanthus roseus (L. G. Don

    Directory of Open Access Journals (Sweden)

    Agnieszka Pietrosiuk

    2014-02-01

    Full Text Available The effect of an ethanolic extract from callus of Catharanthus roseus on Allium cepa root cells divisions was investigated. Two lines: white and green callus, were established on solid B5 medium with IAA 1 mg/l and kinetin 0.1 mg/l. The HPLC analysis of callus extracts showed the presence of indole alkaloids, however not known pharmacologically active alkaloids or derivatives used in semisynthesis (vinblastine, ajmalicine, serpentine, yohimbine, vindoline and catharanthine have been found. The ethanolic extract of C. roseus callus inhibited the number of mitoses in Allium cepa root tip cells. Short (1-3 hours treatment resulted in an increase in the index of late prophases, with characteristic light spaces, and the index of metaphases with twisted chromosomes forming an equatorial plate or irregular structures of c-metaphases. At the same time, the percentage of anaphases and telophases decreased significantly. Longer treatment of the root tip inhibited mitotic activity, stopping it completely already after 12 hours. Interphase nuclei became at first denser and homogeneous, eventually their structure became , partitioned into zones and formation of chromatin territories, with distinct large nucleoli has been observed. Electron microscope observations revealed well developed rough endoplasmic reticulum and thick, invaginating cell membrane.

  4. Don Zhuanu otdajutsja tolko po ljubvi

    Index Scriptorium Estoniae

    2006-01-01

    Eestisse tuleb külla Roman Viktjuki Teater, 1. nov. mängitakse Vene Kultuurikeskuses Jean Genet' "Toatüdrukuid" (lavastaja Roman Viktjuk), 23. nov. etendatakse Eric-Emmanuel Scmitti "Don Juani viimast armastust" (lavastaja Roman Viktjuk)

  5. Donning and doffing PPE for HPAI surveillance

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — Instructions describing the proper donning and doffing personal protective equipment (PPE) for highly pathogenic avian influenza (HPAI) surveillance. This protocol...

  6. Don't Take This with That!

    Medline Plus

    Full Text Available ... Resources for You Special Features Don't take this with that! Share Tweet Linkedin Pin it More ... in the intestine that help absorb drugs. When this happens, less of the drug reaches the bloodstream ...

  7. Don't Take This with That!

    Medline Plus

    Full Text Available ... Health and Human Services FDA U.S. Food and Drug Administration Protecting and Promoting Your Health A to ... Vaccines, Blood & Biologics Animal & Veterinary Cosmetics Tobacco Products Drugs Home Drugs Resources for You Special Features Don' ...

  8. Don't Take This with That!

    Medline Plus

    Full Text Available HHS U.S. Department of Health and Human Services FDA U.S. Food and Drug Administration Protecting and Promoting ... Features Don't take this with that! Share Tweet Linkedin Pin ...

  9. Dry Mouth? Don't Delay Treatment

    Science.gov (United States)

    ... For Consumers Home For Consumers Consumer Updates Dry Mouth? Don't Delay Treatment Share Tweet Linkedin Pin ... saliva, cavities may occur. back to top Dry Mouth Treatments Your doctor or dentist may recommend oral ...

  10. Educational background and professional participation by federal wildlife biologists: Implications for science, management, and The Wildlife Society

    Science.gov (United States)

    Schmutz, J.A.

    2002-01-01

    Over 2,000 people are employed in wildlife biology in the United States federal government. The size of this constituency motivated me to examine the amount of formal education federal biologists have received and the extent of continuing education they undertake by reading journals or attending scientific meetings. Most federal biologists who are members of The Wildlife Society (TWS) have a graduate degree. However, one-third have only a Bachelor of Science degree, despite the current trend toward hiring people with graduate degrees. Most federal biologists are not research biologists. Numbers of journals subscribed to was positively related to educational level. Less than one-third of all wildlife biologists employed by the United States Fish and Wildlife Service are members of TWS or subscribe to any of its journals. In contrast, the majority of presenters at the TWS 2000 Annual Conference were research biologists and members of TWS. The failure of many federal wildlife biologists to read scientific literature or attend professional meetings indicates a failure to promote the importance of continuing education in the federal workplace. I identify 2 potential adverse impacts of this failing: an inability to recognize important and relevant scientific contributions and an ineffectiveness in carrying out adaptive management.

  11. Holes in the Nuclear Membrane as an Illustration of Gaps in the Understanding of the Biology by Biologists.

    Science.gov (United States)

    Kuvichkin, Vasily

    2015-08-01

    At the moment, the conditions are in place to describe how to construct nuclear pores and how they work, missing only real understanding of process. The DNA-RNA-protein paradigm proposed by Crick 53 years ago (Symp Soc Exp Biol 12:138-163, 1958; Nature 227:561-563, 1970) severely hampers our understanding of nuclear pore structure and assembly because the problem lies outside paradigm. DNA in this scheme only plays the role of information storage from which information is transferred to RNA, then from RNA to proteins after which proteins perform all of the functions in the cell. Although it is known that DNA is able to build nucleosomes in vivo, many in vitro structures types of origami (Rothemund, Nature 440:297-302, 2006), the DNA is considered to be exotic as structural material for cells. The structural role of RNA is difficult to ignore, in connections with their participation in structures of ribosomes, ribonucleoproteins, and ribozymes, but imagine that DNA performs an important structural role in the cell is impossible in opinion of many authors. So, when there was a problem in explaining the origin of the nuclear pore, all efforts of biologists were directed to proteins such as nucleoporins, especially when taking into account that there are 30 nucleoporins and only one DNA. Here, I try to explain the typical mistakes of the old approach to such a complex problem as nuclear pore structure and assembly. PMID:25758231

  12. Le paradigme du don face aux nouvelles réalités du monde du travail

    OpenAIRE

    Caillé, Alain

    2012-01-01

    Alain Caillé resitue le paradigme du don que le Mouvement anti-utilitariste en sciences sociales (MAUSS) s’emploie à dégager depuis une trentaine d’années avant de montrer comment les lunettes du don permettent de mieux voir ce qu’il y a dans la boîte noire des organisations et de mieux lire par là même les ravages du néomanagement. À cette contribution répond celle de Norbert Alter dans le cadre du dossier Chorus « Peut-on penser le travail par le don ? »

  13. Fighting for life: Religion and science in the work of fish and wildlife biologists

    Science.gov (United States)

    Geffen, Joel Phillip

    Philosophers, historians, and sociologists of science have argued that it is impossible to separate fact from value. Even so, Americans generally demand that scientists be "objective." No bias is permitted in their work. Religious motivations in particular are widely considered anathema within the halls of science. My dissertation addresses both theoretical and practical aspects concerning objectivity in science through an examination of fish and wildlife biologists. I hypothesized that they use the language of objective science as a tool to convince others to protect habitats and species. Further, I claimed that this "rhetoric of science" is employed either consciously or unconsciously on behalf of personal values, and that religious and/or spiritual values figure significantly among these. Regarding the issue's practical applications, I argued in support of Susan Longino's assertion that while subjective influences exist in science, they do not necessarily indicate that objectivity has been sacrificed. My primary methodology is ethnographic. Thirty-five biologists working in the Pacific Northwest were interviewed during the course of summer 2001. Participant ages ranged from 23 to 78. Both genders were represented, as were various ethnic and cultural backgrounds, including Native American. I used a questionnaire to guide respondents through a consistent set of open-ended queries. I organized their answers under four categories: the true, the good, the beautiful, and the holy. The first three were borrowed from the theoretical writings of philosopher Immanuel Kant. The last came from Rudolf Otto's theological work. These categories provided an excellent analytical framework. I found that the great majority of fish and wildlife biologists strive for objectivity. However, they are also informed by powerful contextual values. These are derived from environmental ethics, aesthetic preferences pertaining to ecosystem appearance and function, and visceral experiences of

  14. Speak Up! But Don't Strain Your Voice

    Science.gov (United States)

    ... Home Current Issue Past Issues Hearing Disorders Speak Up! But don't strain your voice Past Issues / ... Noise Exposure / How Loud Is Too Loud? / Speak Up! But don't strain your voice / Medical Mystery: ...

  15. Sleeping and Driving Don't Mix: Sleep Quiz

    Science.gov (United States)

    ... Don't Mix Sleeping and Driving Don't Mix Primary tabs View (active tab) Take if ($view_ ... Sign up for our Email Newsletter For Email Marketing you can trust Footer Contact Info Home | Contact | ...

  16. A FASTQ compressor based on integer-mapped k-mer indexing for biologist.

    Science.gov (United States)

    Zhang, Yeting; Patel, Khyati; Endrawis, Tony; Bowers, Autumn; Sun, Yazhou

    2016-03-15

    Next generation sequencing (NGS) technologies have gained considerable popularity among biologists. For example, RNA-seq, which provides both genomic and functional information, has been widely used by recent functional and evolutionary studies, especially in non-model organisms. However, storing and transmitting these large data sets (primarily in FASTQ format) have become genuine challenges, especially for biologists with little informatics experience. Data compression is thus a necessity. KIC, a FASTQ compressor based on a new integer-mapped k-mer indexing method, was developed (available at http://www.ysunlab.org/kic.jsp). It offers high compression ratio on sequence data, outstanding user-friendliness with graphic user interfaces, and proven reliability. Evaluated on multiple large RNA-seq data sets from both human and plants, it was found that the compression ratio of KIC had exceeded all major generic compressors, and was comparable to those of the latest dedicated compressors. KIC enables researchers with minimal informatics training to take advantage of the latest sequence compression technologies, easily manage large FASTQ data sets, and reduce storage and transmission cost. PMID:26743127

  17. Don't Ask, Don't Tell: A Costly and Wasteful Policy 

    OpenAIRE

    Barnes, Johnny L.

    2004-01-01

    Since the current policy known as "Don't Ask, Don't Tell" was adopted in the early 1990s, several changes have taken place which call into question the policy's validity. Firstly, the argument proponents of the ban use to justify it, namely that cohesion would suffer if admitted homosexuals were allowed to serve, has been undercut by social science analyses on the correlation between cohesion and performance. Their argument has also been undercut by empirical evidence from several nations tha...

  18. Urinary deoxynivalenol (DON) and zearalenone (ZEA) as biomarkers of DON and ZEA exposure of pigs.

    Science.gov (United States)

    Thanner, S; Czeglédi, L; Schwartz-Zimmermann, H E; Berthiller, F; Gutzwiller, A

    2016-05-01

    Four diets contaminated with 1.1 to 5.0 mg/kg deoxynivalenol (DON) and 0.4 to 2.4 mg/kg zearalenone (ZEA) were fed to four groups of six growing Large White pigs. Urine samples were collected after 3 to 4 days and again after 6 to 7 days on the diets. On each sampling day, half of the animals were sampled in the morning, after an 8-h fast, and the other half were sampled in the afternoon, after 7 h of ad libitum access to feed. The urinary concentrations of DON, DON-glucuronide, DON-3-sulphate, de-epoxy-DON, as well as of ZEA, ZEA-14-glucuronide, α-zearalenol and α-zearalenol-14-glucuronide, analysed using LC-MS/MS, were used to calculate urinary DON and ZEA equivalent concentrations (DONe and ZEAe). The urinary concentration of DONe (P intake per kg body weight the day preceding sampling (r = 0.76 and 0.77; P intake during the 7 h preceding urine sampling in the afternoon and urinary DONe/creatinine ratio (r = 0.88) as well as between mean ZEA intake during 3 days preceding urine sampling and urinary ZEAe/creatinine ratio (r = 0.84) were even higher, reflecting the plasma elimination half-time of several hours for DON and of more than 3 days for ZEA. ZEAe analysed in enzymatically hydrolysed urine using an ELISA kit was highly correlated with the LC-MS/MS data (r = 0.94). The urinary DONe and ZEAe to creatinine ratios, analysed in pooled urine samples of several pigs fed the same diet, can be used to estimate their exposure to DON and ZEA. PMID:26888520

  19. Don Juan on manipulaator / Kai Ilustrumm

    Index Scriptorium Estoniae

    Ilustrumm, Kai

    2004-01-01

    Nüüdisaegne muusikalis-koreograafiline fantaasia "Don Juani pidusöök" Kanuti Gildi Saalis. Eesti tantsijate ja hollandi muusikute Holland Windplayers koostöös sündinud projekti tutvustab Tallinna Filharmoonia sarja "Diplomaadilised noodid" juht Heli Vaus-Tamm

  20. Don't Take This with That!

    Medline Plus

    Full Text Available ... En Español Search FDA Submit search Popular Content Home Food Drugs Medical Devices Radiation-Emitting Products Vaccines, Blood & Biologics Animal & Veterinary Cosmetics Tobacco Products Drugs Home Drugs Resources for You Special Features Don't ...

  1. Speaking Personally--With Don Knezek

    Science.gov (United States)

    American Journal of Distance Education, 2009

    2009-01-01

    This article presents an interview with Don Knezek, CEO of International Society for Technology in Education (ISTE). Knezek is a leader in coupling technology and creativity to transform education. His experience and impact span local to global levels--ranging from district-wide, classroom-based innovation and far-reaching, multi-state projects to…

  2. Saga y fugas de Don Juan

    OpenAIRE

    1998-01-01

    Ensayo acerca de las diversas versiones e interpretaciones que a lo largo de la historia ha suscitado el mito de Don Juan Tenorio. Lectura original del donjuanismo a partir de las teorías del filósofo José Antonio Marina en torno al ingenio y sus productos.

  3. Don Quixote at 400: Still Conquering Hearts

    Science.gov (United States)

    Stavans, Ilan

    2005-01-01

    The Knight of the Sorrowful Countenance is turning 400. By some accounts, the first part of Don Quixote, Miguel de Cervantes's masterpiece, was available in Valladolid by Christmas Eve 1604, although Madrid did not get copies until January 1605. Thus came to life the "ingenious gentleman" who, ill equipped with antiquated armor "stained with rust…

  4. BioLogistics and the Struggle for Efficiency: Concepts and Perspectives

    CERN Document Server

    Helbing, Dirk; Diez, Stefan; Peters, Karsten; Kalaidzidis, Yannis; Padberg, Kathrin; Lammer, Stefan; Johansson, Anders; Breier, Georg; Schulze, Frank; Zerial, Marino; 10.1142/S0219525909002374

    2010-01-01

    The growth of world population, limitation of resources, economic problems and environmental issues force engineers to develop increasingly efficient solutions for logistic systems. Pure optimization for efficiency, however, has often led to technical solutions that are vulnerable to variations in supply and demand, and to perturbations. In contrast, nature already provides a large variety of efficient, flexible and robust logistic solutions. Can we utilize biological principles to design systems, which can flexibly adapt to hardly predictable, fluctuating conditions? We propose a bio-inspired "BioLogistics" approach to deduce dynamic organization processes and principles of adaptive self-control from biological systems, and to transfer them to man-made logistics (including nanologistics), using principles of modularity, self-assembly, self-organization, and decentralized coordination. Conversely, logistic models can help revealing the logic of biological processes at the systems level.

  5. CWM Global Search—The Internet Search Engine for Chemists and Biologists

    Directory of Open Access Journals (Sweden)

    Hans-Jürgen Himmler

    2010-12-01

    Full Text Available CWM Global Search is a meta-search engine allowing chemists and biologists to search the major chemical and biological databases on the Internet, by structure, synonyms, CAS Registry Numbers and free text. A meta-search engine is a search tool that sends user requests to several other search engines and/or databases and aggregates the results into a single list or displays them according to their source [1]. CWM Global Search is a web application that has many of the characteristics of desktop applications (also known as Rich Internet Application, RIA, and it runs on both Windows and Macintosh platforms. The application is one of the first RIA for scientists. The application can be started using the URL http://cwmglobalsearch.com/gsweb.

  6. What in silico molecular docking can do for the `bench-working biologists'

    Indian Academy of Sciences (India)

    Marius Mihăşan

    2012-12-01

    Required by an increasing amount of scientists, the in silico docking field is in full expansion, new algorithms and methods appearing at an exponential rate. The sheer range of available programs is overwhelming for the bench-working biologist, which is often discouraging by the lack of a graphical user interface, good user manual or literature to validate a given program. This mini-review attempts to present the docking problem and available solutions from a non-bioinformatician point of view and makes a selection of the available servers and programs. These tools are evaluated from several points of view, as numbers of citations, ease of usage and computer requirements. Finally, the capabilities and limitations as well as specific applications of in silico docking techniques are presented.

  7. GeneNotes – A novel information management software for biologists

    Directory of Open Access Journals (Sweden)

    Wong Wing H

    2005-02-01

    Full Text Available Abstract Background Collecting and managing information is a challenging task in a genome-wide profiling research project. Most databases and online computational tools require a direct human involvement. Information and computational results are presented in various multimedia formats (e.g., text, image, PDF, word files, etc., many of which cannot be automatically processed by computers in biologically meaningful ways. In addition, the quality of computational results is far from perfect and requires nontrivial manual examination. The timely selection, integration and interpretation of heterogeneous biological information still heavily rely on the sensibility of biologists. Biologists often feel overwhelmed by the huge amount of and the great diversity of distributed heterogeneous biological information. Description We developed an information management application called GeneNotes. GeneNotes is the first application that allows users to collect and manage multimedia biological information about genes/ESTs. GeneNotes provides an integrated environment for users to surf the Internet, collect notes for genes/ESTs, and retrieve notes. GeneNotes is supported by a server that integrates gene annotations from many major databases (e.g., HGNC, MGI, etc.. GeneNotes uses the integrated gene annotations to (a identify genes given various types of gene IDs (e.g., RefSeq ID, GenBank ID, etc., and (b provide quick views of genes. GeneNotes is free for academic usage. The program and the tutorials are available at: http://bayes.fas.harvard.edu/genenotes/. Conclusions GeneNotes provides a novel human-computer interface to assist researchers to collect and manage biological information. It also provides a platform for studying how users behave when they manipulate biological information. The results of such study can lead to innovation of more intelligent human-computer interfaces that greatly shorten the cycle of biology research.

  8. ASPECTS CONCERNING THE EFFECT OF SOME ANTIOXIDANTS ON LIVER HISTOLOGICAL STRUCTURE IN WEANED PIGS, INTOXICATED WITH DEOXYNIVALENOL (DON

    Directory of Open Access Journals (Sweden)

    GABI DUMITRESCU

    2013-12-01

    Full Text Available Deoxynivalenol is a mycotoxin produced by fungi of the Fusarium genera, which are abundant in various cereal crops and processed grains. In order to protect cell structure within the tissues studied, we tried various experimental variants by incorporating some selenium and boron sources into the combined fodder, and also in mixture with DON with different concentrations. To determine the morphophysiological changes induced by the mycotoxin DON upon liver histological structure, and also the antioxidants’ effects, we took samples from 9 piglets belonging to three groups: group 1 (fed with fodder added with DON, group 2 (fed with fodder added with selenium and group 3 (fed with fodder added with selenium and DON. In the case of the individuals in groups 2 and 3 fed with fodder added with addition of selenium, respectively of DON and selenium, the microscopic images reveal the presence of uniform hepatic lobules, delimited between them through fine conjunctive septa. The intralobular capillary network is hypertrophic. The selenium added in the fodder for the individuals from group 3 has a hepatoprotective role, aspect suggested by dystrophic processes occurred on small areas, caused by the presence of DON and by the vascular network’s hypertrophy. In the case of this group, we observed a strong stimulation on the defense system, suggested by the presence of the leukocyte infiltrates within the interlobular conjunctive and by the vascular network’s hypertrophy.

  9. Don quijote, Christelike geloof en natuurwetenskap 1

    OpenAIRE

    H.P.P. Lötter

    1987-01-01

    The story about Don Qulchote and the windmills is used in this article as an analogy to typify the debates that have taken place in history between natural science and representatives of Christian religion. The analogy is tested on debates that took place around Galileo. Darwin, Newton, Cuvier and the Big Bang theory. The analogy matches these debates quite well and this Is explained by the view that scientific claims to knowledge, and claims to knowledge based on Christian faith, belong to d...

  10. Delbrueck Prize Talk: ``I Don't Believe a Word of It'': My Struggles With Max Delbruck's Ghost

    Science.gov (United States)

    Austin, Robert

    2014-03-01

    I never meet Max Delbruck, but I have worked with similarly great biological physicists. Because of this, I am familiar with the expression ``I don't believe a word of it'' that Max Delbruck was known to say, and other great biological physicists have been known to say. Lately I have been working on evolution dynamics and how one might accelerate evolution of de novo mutations by standing on the shoulders of great theoretical evolution biologists such as Sewall Wright and Conrad Waddington and following their insights. I have found Delbruck's pronouncement ``I don't believe a word of it'' ringing in my ears. I will try to convince the ghost of Delbruck and his present day acolytes that Darwin did not have the final say on evolution, that biological organisms are quite creative when it comes to evolution of new traits, and that failure to understand this complexity in evolution has had a major impact on our struggles with the physics of cancer.

  11. From glass flowers to computer games: Examining the emergent media practices of plant biologists

    Science.gov (United States)

    Reitmeyer, Morgan

    The goal of this project is to begin investigating the emergent media practices of current academic disciplines. This dissertation posits that Writing Across the Curriculum (WAC) scholars have investigated new media use in undergraduate pedagogy, and to some extent the practices of graduate students. However, WAC scholars have yet to try to understand how Writing in the Disciplines (WID) is being impacted by emergent media. The gap might be, in part, accounted for by the complexity of researching disciplinary writing compounded by the complexity of researching new media. However, in order to both design effective undergraduate curriculum and partner with peers across the discipline, this research must take place. This dissertation proposes a mixed method process, consisting of textual analysis, interviews and observations, in order to understand emergent media writing in the disciplines. Specifically, this research focuses on the practices of plant biologists online publication, online professionalization, and changing use of databases. This preliminary study reflects on the research practices that could be used to gather this information, as well what rhetoric and composition scholars have to gain from this type of difficult work.

  12. Conscience dilemma: to become a bioengineer or to survive as a biologist.

    Science.gov (United States)

    Selimoglu, Sureyya Mert

    2014-01-01

    Bioengineering is the consideration of biological problems from modern engineering, therefore money-oriented, perspective. Today, grant-giving bodies always favor bioengineering projects rather than pure biology projects (like those in ecology, entomology, etc.). Therefore, today's biologist is forced to be on the horns of a dilemma. They have to either submit a very powerful and valid reason for the proposal of their project, or change the project to one having a potential of money-based outcome. On the other hand, because of dealing with the living components of nature, conducting a research in pure biology is like a kind of worship. For this reason, from a believer scientist's view, a deviation (in terms of research) from biology to bioengineering can be considered like committing a sin. Unfortunately, today's wild capitalism has been bringing new sinners day by day, and this system will continue for the foreseeable future unless grant-giving bodies comprehend the real importance of pure biology. PMID:25936219

  13. Don't be such a scientist talking substance in an age of style

    CERN Document Server

    Olson, Randy

    2009-01-01

    How to relate scientific information in a way that entertains and enlightens? After twenty years as a marine biologist, a Ph.D. from Harvard, a tenured professorship at the University of New Hampshire, and more than twenty published research papers, why is Dr. Randy Olson telling readers, 'Don't Be Such a Scientist'? The hard-earned advice comes from a fifteen-year career transition to filmmaking, culminating in the acclaimed documentaries "Flock of Dodos: The Evolution-Intelligent Design Circus" and "Sizzle: A Global Warming Comedy". In his first book, Olson makes clear what those glued to their microscopes and climate models may not recognize: the general public doesn't speak science. For better or worse, most of us rely not on intellect, but on what Olson terms the organs of mass communication: the heart, the gut, and even 'the lower organs'. Why else do some still question the existence of global warming or evolution when the evidence is irrefutable? Drawing on his own hilarious - and at times humiliating...

  14. Don Quijote, el escribidor y el escritor

    OpenAIRE

    Giovanna Pollarolo

    2010-01-01

    In his diverse critical studies, Mario Vargas Llosa has shown particular interest in reflecting on Miguel de Cervantes’s novelistic theory in accordance with his own concerns as a scholar and novelist. Suffice it to read the essay “Una novela para el siglo XXI” that he wrote in 2005 as an introduction to the edition of Don Quijote prepared to celebrate the IV Centenario, to make sure that Vargas Llosa “reads” Cervantes in accordance with his own reflections on the theory of the novel, fanatic...

  15. Transgender people in the military: don't ask? Don't tell? Don't enlist!

    Science.gov (United States)

    Yerke, Adam F; Mitchell, Valory

    2013-01-01

    The repeal of Don't Ask, Don't Tell offered legal equality to sexual minorities in the military. However, this big step forward had no impact on the policy of exclusion and rejection and the fear and secrecy that resulted for transgender people (whether lesbian, gay, bisexual, or heterosexual). In this article, we argue that transgender citizens should have equal opportunity to honorably serve their country, and to be treated with respect and sensitivity as they do so. Many transgender persons may be drawn to military service and its ethos of masculine values. However, they are currently not permitted entry, and, if they are to enter, must remain hidden or face dismissal, leaving them vulnerable to harassment. While they report both positive and negative experiences during their service, research documents discrimination in veterans' healthcare as well as mental health risks resulting from fear and harassment. In contrast to the United States, 11 countries include transgender people in their militaries. Drawing in part from their examples, we end with recommendations for change in the direction of respect and equality of opportunity. PMID:23414281

  16. Le paradigme du don face aux nouvelles réalités du monde du travail The gift paradigm facing new realities in the work world El paradigma del don frente a las nuevas realidades del mundo del trabajo

    OpenAIRE

    Alain Caillé

    2012-01-01

    Alain Caillé resitue le paradigme du don que le Mouvement anti-utilitariste en sciences sociales (MAUSS) s’emploie à dégager depuis une trentaine d’années avant de montrer comment les lunettes du don permettent de mieux voir ce qu’il y a dans la boîte noire des organisations et de mieux lire par là même les ravages du néomanagement. À cette contribution répond celle de Norbert Alter dans le cadre du dossier Chorus « Peut-on penser le travail par le don ? »Alain Caillé starts by resituating th...

  17. Measuring the continuum linear polarization with ESPaDOnS

    CERN Document Server

    Pereyra, A; Martioli, E

    2014-01-01

    Our goal is to test the feasibility to obtain accurate measurements of the continuum linear polarization from high-resolution spectra using the spectropolarimetric mode of ESPaDOnS. We used the new pipeline OPERA to reduce recent and archived ESPaDOnS data. A couple of standard polarization stars and several science objects were tested. Synthetic broad-band polarization was computed from the ESPaDOnS continuum linear polarization spectra and compared with published values to quantify the accuracy of the instrument. The continuum linear polarization measured by ESPaDOnS is consistent with the broad-band polarimetry measurements available in the literature. The polarization degree accuracy is better than 0.2% considering the full sample. The accuracy in polarization position angle using the most polarized objects is better than 5deg. Our results suggest that measurements of the continuum linear polarization using ESPaDOnS are viable.

  18. Cognitive empathy in inter-disciplinary research: the contrasting attitudes of plant breeders and molecular biologists towards rice

    Indian Academy of Sciences (India)

    E Haribabu

    2000-12-01

    I draw attention to the perceptions of and interactions between molecular biologists and scientists engaged in plant breeding in India, who have been attempting to employ molecular biology tools to understand and intervene to improve the rice crop. The present essay suggests that the concept of cognitive empathy is crucial for enabling basic scientists and applied scientists to begin to understand phenomena from the point of view of the other and from the point of view of society at large, and in arriving at solutions that are scientifically feasible and socially acceptable. Socialization into disciplinary cultures, organizational factors and individual anxieties seem to inhibit inter-disciplinary collaboration. The majority of rice breeders and a small group of molecular biologists emphasize the relative merits of marker-assisted selection (MAS) in the near term vis-à-vis the currently controversial transgenic approach for rice crop improvement in India.

  19. Don quijote, Christelike geloof en natuurwetenskap 1

    Directory of Open Access Journals (Sweden)

    H. P.P. Lötter

    1987-03-01

    Full Text Available The story about Don Qulchote and the windmills is used in this article as an analogy to typify the debates that have taken place in history between natural science and representatives of Christian religion. The analogy is tested on debates that took place around Galileo. Darwin, Newton, Cuvier and the Big Bang theory. The analogy matches these debates quite well and this Is explained by the view that scientific claims to knowledge, and claims to knowledge based on Christian faith, belong to different categories and are thus not concurrents for the same truth. Gilbert Ryle's criticism on Descartes' mind-body dualism is used here to strengthen this argument. The article is concluded by pointing out in what ways one can engage in a meaningful debate on the nature of the relationship between science and Christian religion.

  20. We don't need another hero.

    Science.gov (United States)

    Badaracco, J L

    2001-09-01

    Everybody loves the stories of heroes like Martin Luther King, Jr., Mother Teresa, and Gandhi. But the heroic model of moral leadership usually doesn't work in the corporate world. Modesty and restraint are largely responsible for the achievements of the most effective moral leaders in business. The author, a specialist in business ethics, says the quiet leaders he has studied follow four basic rules in meeting ethical challenges and making decisions. The rules constitute an important resource for executives who want to encourage the development of such leaders among their middle managers. The first rule is "Put things off till tomorrow." The passage of time allows turbulent waters to calm and lets leaders' moral instincts emerge. "Pick your battles" means that quiet leaders don't waste political capital on fights they can't win; they save it for occasions when they really want to fight. "Bend the rules, don't break them" sounds easier than it is--bending the rules in order to resolve a complicated situation requires imagination, discipline, restraint, flexibility, and entrepreneurship. The fourth rule, "Find a compromise," reflects the author's finding that quiet leaders try not to see situations as polarized tests of ethical principles. These individuals work hard to craft compromises that are "good enough"--responsible and workable enough--to satisfy themselves, their companies, and their customers. The vast majority of difficult problems are solved through the consistent striving of people working far from the limelight. Their quiet approach to leadership doesn't inspire, thrill, or provide story lines for uplifting TV shows. But the unglamorous efforts of quiet leaders make a tremendous difference every day in the corporate world. PMID:11550628

  1. Odgojno očinstvo. Don Boscova pouka

    OpenAIRE

    Giraudo, Aldo

    2007-01-01

    Podsjećajući na autobiografski don Boscov zapis o bolnom prisjećanju na smrt vlastita oca u ranom djetinjstvu, autor razmišlja o don Boscu kao duhovnom ocu mladih. Očinski lik ima važnu ulogu u don Boscovu odgajateljskom životu i djelovanju. U njegovu odgojnom sustavu posebno mjesto zauzima empatijska suosjećajnost s odgajanikom. Tako se između odgajatelja i odgajanika razvija specifična komunikacija po kojoj se odgajanika upućuje na prikladan put prema njemu svojstvenom rastu i zrelosti. Odg...

  2. Adults Don't Need Tetanus Shot Every Decade

    Science.gov (United States)

    ... fullstory_157980.html Adults Don't Need Tetanus Shot Every Decade: Study Booster shot every 30 years would protect as well as ... have always been told to get a tetanus shot every 10 years, but actually, there is very ...

  3. Hyperbaric Oxygen Therapy: Don't Be Misled

    Science.gov (United States)

    ... For Consumers Home For Consumers Consumer Updates Hyperbaric Oxygen Therapy: Don't Be Misled Share Tweet Linkedin ... HBOT Works What are the Risks No, hyperbaric oxygen therapy (HBOT) has not been clinically proven to ...

  4. Most Pediatricians Don't Ask about Mom's Depression

    Science.gov (United States)

    ... html Most Pediatricians Don't Ask About Mom's Depression About 4 in 10 moms with young kids ... pediatricians in the United States ask mothers about depression, even though the condition affects many women with ...

  5. News from the CFHT/ESPaDOnS spectropolarimeter

    CERN Document Server

    Moutou, C; Manset, N; Selliez-Vandernotte, L; Desrochers, M -E

    2015-01-01

    The ESPaDOnS spectropolarimeter has been in use on the Canada-France-Hawaii Telescope (CFHT) since 2004, for studying stars, galactic objects and planets. ESPaDOnS is used in queued service observing mode since 2008, which allows an optimization of the science outcome. In this article, we summarize the new functionalities and analyses made on ESPaDOnS operations and data for the present and future users. These modifications include: signal-to-noise ratio based observing, radial velocity nightly drifts, the OPERA pipeline under development, the measurement of H2O content in the Maunakea sky, and the use of ESPaDOnS with the neighbour telescope Gemini.

  6. Don't Blame Kids' Behavior on Full Moon

    Science.gov (United States)

    ... html Don't Blame Kids' Behavior on Full Moon International study puts common myth to rest To ... parents swear their children's behavior changes when the moon is full, but new research suggests otherwise. "Our ...

  7. Will 3552 Don Quixote escape from the Solar System?

    Directory of Open Access Journals (Sweden)

    Suryadi Siregar

    2011-05-01

    Full Text Available Asteroid 1983 SA, well known as 3552 Don Quixote, is one of Near Earth Asteroids (NEAs which is the most probable candidate for the cometary origin, or otherwise as Jupiter-Family-Comets (JFCs. The aim of this study is to investigate the possibility of 3552 Don Quixote to be ejected from the Solar System. This paper presents an orbital evolution of 100 hypothetical asteroids generated by cloning 3552 Don Quixote. Investigation of its orbital evolution is conducted by using the SWIFT subroutine package, where the gravitational perturbations of eight major planets in the Solar System are considered. Over very short time scales (220 kyr relative to the Solar System life time (10 Gyr, the asteroid 3552 Don Quixote gave an example of chaotic motion that can cause asteroid to move outward and may be followed by escaping from the Solar System. Probability of ejection within the 220 kyr time scale is 50%.

  8. Speak Up! But don't strain your voice

    Science.gov (United States)

    ... Disorders Speak Up! But don't strain your voice Past Issues / Fall 2008 Table of Contents For ... Javascript on. A clinical trial at the NIDCD Voice Center gave Sherdina Jones tools to limit voice ...

  9. News from the CFHT/ESPaDOnS spectropolarimeter

    Science.gov (United States)

    Moutou, C.; Malo, L.; Manset, N.; Selliez-Vandernotte, L.; Desrochers, M.-E.

    2015-12-01

    The ESPaDOnS spectropolarimeter has been in use on the Canada-France-Hawaii Telescope (CFHT) since 2004, for studying stars, galactic objects and planets. ESPaDOnS is used in queued service observing mode since 2008, which allows an optimization of the science outcome. In this article, we summarize the new functionalities and analyses made on ESPaDOnS operations and data for the present and future users. These modifications include: signal-to-noise ratio based observing, radial velocity nightly drifts, the OPERA pipeline under development, the measurement of H2O content in the Maunakea sky, and the use of ESPaDOnS with the neighbour telescope Gemini.

  10. Stem cells: a plant biology perspective

    NARCIS (Netherlands)

    Scheres, B.J.G.

    2005-01-01

    A recent meeting at the Juan March Foundation in Madrid, Spain brought together plant biologists to discuss the characteristics of plant stem cells that are unique and those that are shared by stem cells from the animal kingdom

  11. Microfluidic tools for cell biological research

    OpenAIRE

    Velve-Casquillas, Guilhem; Le Berre, Maël; Piel, Matthieu; Tran, Phong T.

    2010-01-01

    Microfluidic technology is creating powerful tools for cell biologists to control the complete cellular microenvironment, leading to new questions and new discoveries. We review here the basic concepts and methodologies in designing microfluidic devices, and their diverse cell biological applications.

  12. Quantum Physics for Scientists and Technologists Fundamental Principles and Applications for Biologists, Chemists, Computer Scientists, and Nanotechnologists

    CERN Document Server

    Sanghera, Paul

    2011-01-01

    Presenting quantum physics for the non-physicists, Quantum Physics for Scientists and Technologists is a self-contained, cohesive, concise, yet comprehensive, story of quantum physics from the fields of science and technology, including computer science, biology, chemistry, and nanotechnology. The authors explain the concepts and phenomena in a practical fashion with only a minimum amount of math. Examples from, and references to, computer science, biology, chemistry, and nanotechnology throughout the book make the material accessible to biologists, chemists, computer scientists, and non-techn

  13. Extraction of High Quality RNA from Cannabis sativa Bast Fibres: A Vademecum for Molecular Biologists

    OpenAIRE

    Gea Guerriero; Lauralie Mangeot-Peter; Jean-Francois Hausman; Sylvain Legay

    2016-01-01

    In plants there is no universal protocol for RNA extraction, since optimizations are required depending on the species, tissues and developmental stages. Some plants/tissues are rich in secondary metabolites or synthesize thick cell walls, which hinder an efficient RNA extraction. One such example is bast fibres, long extraxylary cells characterized by a thick cellulosic cell wall. Given the economic importance of bast fibres, which are used in the textile sector, as well as in biocomposites ...

  14. Empirical investigation of the ethical reasoning of physicians and molecular biologists – the importance of the four principles of biomedical ethics

    Directory of Open Access Journals (Sweden)

    Ebbesen Mette

    2007-10-01

    Full Text Available Abstract Background This study presents an empirical investigation of the ethical reasoning and ethical issues at stake in the daily work of physicians and molecular biologists in Denmark. The aim of this study was to test empirically whether there is a difference in ethical considerations and principles between Danish physicians and Danish molecular biologists, and whether the bioethical principles of the American bioethicists Tom L. Beauchamp and James F. Childress are applicable to these groups. Method This study is based on 12 semi-structured interviews with three groups of respondents: a group of oncology physicians working in a clinic at a public hospital and two groups of molecular biologists conducting basic research, one group employed at a public university and the other in a private biopharmaceutical company. Results In this sample, the authors found that oncology physicians and molecular biologists employed in a private biopharmaceutical company have the specific principle of beneficence in mind in their daily work. Both groups are motivated to help sick patients. According to the study, molecular biologists explicitly consider nonmaleficence in relation to the environment, the researchers' own health, and animal models; and only implicitly in relation to patients or human subjects. In contrast, considerations of nonmaleficence by oncology physicians relate to patients or human subjects. Physicians and molecular biologists both consider the principle of respect for autonomy as a negative obligation in the sense that informed consent of patients should be respected. However, in contrast to molecular biologists, physicians experience the principle of respect for autonomy as a positive obligation as the physician, in dialogue with the patient, offers a medical prognosis based upon the patients wishes and ideas, mutual understanding, and respect. Finally, this study discloses utilitarian characteristics in the overall conception of

  15. Don't neglect routine staff meetings.

    Science.gov (United States)

    Board, H K

    1982-03-01

    Staff meetings are essential to good staff communication. Meetings help keep the grapevine from growing so big that it strangles the group with its rumors. By holding regular meetings with your staff, you create a consistency in your communications that helps prevent problems that you don't even suspect from cropping up. All personnel should attend the meetings. This way everyone hears news at the same time. Be consistent in your use of meetings. Meetings are more effective if you have a planned agenda and a firm time schedule. Encourage your staff to use meetings to talk out problems that affect the group. Once the meeting is over, encourage them to leave their feelings in the room. Many leaders are reluctant, for a variety of reasons, to hold meetings with their staffs. But it's like dieting and exercise; the more you do it, the easier it becomes. This type of meeting will pay rich dividends in staff personal and professional growth and in improved communication. The sense of participation that can be gained by the effective use of staff meetings can lead to high morale and effective staff performance. As you begin to see the results of a cohesive staff functioning together well, you will realize the routine staff meeting is a management tool that should not be overlooked or underused. PMID:6917733

  16. Don Quijote, el escribidor y el escritor

    Directory of Open Access Journals (Sweden)

    Giovanna Pollarolo

    2010-06-01

    Full Text Available In his diverse critical studies, Mario Vargas Llosa has shown particular interest in reflecting on Miguel de Cervantes’s novelistic theory in accordance with his own concerns as a scholar and novelist. Suffice it to read the essay “Una novela para el siglo XXI” that he wrote in 2005 as an introduction to the edition of Don Quijote prepared to celebrate the IV Centenario, to make sure that Vargas Llosa “reads” Cervantes in accordance with his own reflections on the theory of the novel, fanaticism or the “insanity” of the characters, the fiction and its relationship with “reality”, the narrative voices or the problem of the narrator. But the Cervantes – Vargas Llosa relationship goes beyond the theoretical level or reflective and is manifested in his own novelistic creation such as ocurrs, specifically, in La tía Julia y el escribidor. In that sense, the main goal of this study is to analyze the “dialogue” that is set between La tía Julia y el escribidor and the Quijotefrom the point of view of the “insanity” of Pedro Camacho, the writer of radio soap operas created by Vargas Llosa, and their similarities with that of the knight-errant.

  17. L’utilité de la théorie du don The utility of gift theory Utilidad de la teoría del don

    Directory of Open Access Journals (Sweden)

    Norbert Alter

    2012-12-01

    Full Text Available Norbert Alter explique l’utilité et l’actualité de la théorie de Marcel Mauss pour penser la coopération en entreprise et l’engagement au travail. Il revient sur ses qualités heuristiques et sa portée critique nécessaires à mobiliser pour donner des clefs de compréhension et d’action au monde du travail. À cette contribution répond celle d’Alain Caillé, « Le paradigme du don face aux nouvelles réalités du monde du travail. Quelques remarques »Norbert Alter explains here the utility and topicality of Marcel Mauss’ theory in an analysis touching both on cooperation within a business structure and on people’s commitment to work. He reviews the heuristic qualities and critical scope that must be mobilised to create ways of understanding and acting within the work world. Alain Caillé responds to this contribution with his own article entitled, “ The gift paradigm facing new realities in the work world. Comments” (Le paradigme du don face aux nouvelles réalités du monde du travail. Quelques remarques.Norbert Alter explica la utilidad y la actualidad de la teoría de Marcel Mauss para pensar la cooperación en la empresa y el compromiso en el trabajo. Desde una nueva perspectiva, aborda las cualidades heurísticas de esa teoría y su alcance crítico, que es necesario movilizar para contar con claves de comprensión y de acción en el mundo laboral. A su contribución, Alain Caillé responde con el artículo “El paradigma del don frente a las nuevas realidades del mundo laboral. Algunas observaciones”.

  18. Neutrons for biologists: a beginner's guide, or why you should consider using neutrons

    OpenAIRE

    Lakey, Jeremy H.

    2009-01-01

    From the structures of isolated protein complexes to the molecular dynamics of whole cells, neutron methods can achieve a resolution in complex systems that is inaccessible to other techniques. Biology is fortunate in that it is rich in water and hydrogen, and this allows us to exploit the differential sensitivity of neutrons to this element and its major isotope, deuterium. Furthermore, neutrons exhibit wave properties that allow us to use them in similar ways to light, X-rays and electrons....

  19. Don Quixote e a transgressão do saber

    Directory of Open Access Journals (Sweden)

    Paulo Roberto Ceccarelli

    2009-01-01

    truth will constantly be replaced to make place to another one that, in turn, will also be replaced. Cervantes belongs to the intellectual tradition of Erasmus de Rotterdam, and his Don Quixote rambles thought the universe of Erasmus in which every truth is suspected, and everything bathes in incertitude. Don Quixote de La Mancha is the result of the wisdom of Rotterdam and craziness of La Mancha. By transgressing the "known", Don Quixote transgresses the truth that up to then was taken for sacred and as a guarantee of knowledge and, therefore, of a possible means of controlling the world. Transgression produces turning points but there is not such a thing like a "final transgressing", which would be the equivalent of recreating the myth of an absolute knowledge. According to author, both our theoretical and clinical practices lack the necessary transgressing movement that would force us to abandon the shelter of constructed truths.

  20. Antidiabetic and antioxidant properties of alkaloids from Catharanthus roseus (L.) G. Don.

    Science.gov (United States)

    Tiong, Soon Huat; Looi, Chung Yeng; Hazni, Hazrina; Arya, Aditya; Paydar, Mohammadjavad; Wong, Won Fen; Cheah, Shiau-Chuen; Mustafa, Mohd Rais; Awang, Khalijah

    2013-01-01

    Catharanthus roseus (L.) G. Don is a herbal plant traditionally used by local populations in India, South Africa, China and Malaysia to treat diabetes. The present study reports the in vitro antioxidant and antidiabetic activities of the major alkaloids isolated from Catharanthus roseus (L.) G. Don leaves extract. Four alkaloids--vindoline I, vindolidine II, vindolicine III and vindolinine IV--were isolated and identified from the dichloromethane extract (DE) of this plant's leaves. DE and compounds I-III were not cytotoxic towards pancreatic β-TC6 cells at the highest dosage tested (25.0 µg/mL). All four alkaloids induced relatively high glucose uptake in pancreatic β-TC6 or myoblast C2C12 cells, with III showing the highest activity. In addition, compounds II-IV demonstrated good protein tyrosine phosphatase-1B (PTP-1B) inhibition activity, implying their therapeutic potential against type 2 diabetes. III showed the highest antioxidant potential in ORAC and DPPH assays and it also alleviated H₂O₂-induced oxidative damage in β-TC6 cells at 12.5 µg/mL and 25.0 µg/mL. PMID:23955322

  1. Antidiabetic and Antioxidant Properties of Alkaloids from Catharanthus roseus (L. G. Don

    Directory of Open Access Journals (Sweden)

    Won Fen Wong

    2013-08-01

    Full Text Available Catharanthus roseus (L. G. Don is a herbal plant traditionally used by local populations in India, South Africa, China and Malaysia to treat diabetes. The present study reports the in vitro antioxidant and antidiabetic activities of the major alkaloids isolated from Catharanthus roseus (L. G. Don leaves extract. Four alkaloids—vindoline I, vindolidine II, vindolicine III and vindolinine IV—were isolated and identified from the dichloromethane extract (DE of this plant’s leaves. DE and compounds I–III were not cytotoxic towards pancreatic β-TC6 cells at the highest dosage tested (25.0 µg/mL. All four alkaloids induced relatively high glucose uptake in pancreatic β-TC6 or myoblast C2C12 cells, with III showing the highest activity. In addition, compounds II–IV demonstrated good protein tyrosine phosphatase-1B (PTP-1B inhibition activity, implying their therapeutic potential against type 2 diabetes. III showed the highest antioxidant potential in ORAC and DPPH assays and it also alleviated H2O2-induced oxidative damage in β-TC6 cells at 12.5 µg/mL and 25.0 µg/mL.

  2. Mars and the Mind of Don

    Science.gov (United States)

    Solomon, S. C.

    2002-12-01

    Among the many interests of Don Turcotte have been the terrestrial planets, both as individual bodies and as test beds for generalizations on the processes that have affected the form, evolution, and interior dynamics of Earth-like planets. Over a span of more than 20 years, Turcotte and his collaborators have contributed many insights into the surface evolution and mantle dynamics of Mars on topics ranging from the nature of support of long-wavelength topography, the origin of the Tharsis rise, the internal thermal evolution and its effect on topography and lithospheric stress, and the history of outgassing. A summary of recent findings concerning early Martian evolution permits Turcotte's contributions to be placed within the context of current understanding. Core-mantle differentiation occurred within 10-15 My of the start of solar system evolution, on the basis of isotope systematics in Martian meteorites. A magnetic dynamo was active in the early Noachian but apparently ceased before the end of heavy bombardment. Crustal formation was largely complete by the earliest Noachian, on the basis of the density of both well-preserved and largely buried impact structures. Gravity-topography admittances for Noachian terrain suggests that the general south-to-north thinning of the crust may have been the result of lower crustal flow, but rapid cooling of the crust is indicated by the preservation of early Noachian relief and large crustal magnetization anomalies. The onset of the Tharsis province as a site of voluminous magmatism and concentrated deformation likely postdated the establishment of the crustal thickness dichotomy, but much of the magmatism occurred prior to the formation of late Noachian valley networks. Extensive resurfacing of the northern hemisphere occurred in the Noachian, on the basis of the detection of large impact structures superposed on older filled basins. Extensive evidence for water-surface interactions during this period supports the view

  3. Don Quijote por dentro: el madrigal (DQ II, 68)

    OpenAIRE

    Fernández, J.

    2007-01-01

    El madrigalete que don Quijote canta en la noche, y el contexto en el que aparece (DQ II, 68), proporcionan una clave fundamental (“temor” y “esperanza” para apreciar la evolución tan humana y verdadera del caballero en esta narración de sus aventuras, que constituye una bella metáfora de la vida humana. The “madrigalete” that don Quixote sings in the night and the context in which it appears (DQ II, 68), provides a fundamental clue (“fear” and “hope”) to understand the human evolution of the...

  4. Don Bosco Technical School: A Situational Survey and Strategic Analysis

    OpenAIRE

    Ravasco, Gerard

    2005-01-01

    Don Bosco Technical School in Phnom Penh is a typical example of a long-running non-profit institution in Cambodia. In this part of the world it is plainly called an NGO – a non-governmental organization. It provides vocational skills training education to some four hundred out-of-school youth every year. And yet it does not charge for tuition; nay more it even provides free lunch at noon. So how does Don Bosco Technical School survive this monetary based and profit oriented society? What are...

  5. Pia Petersen sous le signe de don Quichotte

    Directory of Open Access Journals (Sweden)

    Esther Bautista Naranjo

    2013-04-01

    Full Text Available This interview has been motivated by the Cervantean intertextuality of Pia Petersen’s (a French-speaking Danish author last novel, Le Chien de don Quichotte (2012. Departing from the values that the writer attributes to Cervantes’ work, I try to establish a connection with a specific critical trend in order to test, later on, to which extent this approach is used by the author to create a series of characters placed under the sign of don Quixote. Finally, I evaluate her novel’s relation with contemporary reality.

  6. Fuel elements assembling for the DON project exponential experience

    International Nuclear Information System (INIS)

    It is described the fuel unit used in the DON exponential experience, the manufacturing installments and tools as well as the stages in the fabrication.These 74 elements contain each 19 cartridges loaded with synterized urania, uranium carbide and indium, gold, and manganese probes. They were arranged in calandria-like tubes and the process-tube. This last one containing a cooling liquid simulating the reactor organic. Besides being used in the DON reactor exponential experience they were used in critic essays by the substitution method in the French reactor AQUILON II. (Author) 6 refs

  7. CAS Biologists in Shanghai Step up Cooperation with Their West European Peers

    Institute of Scientific and Technical Information of China (English)

    Zhang Qin

    2003-01-01

    @@ Ⅰ. International Cooperation: A Booster for Developing Highlevel Research Bodies The Shanghai Institutes for Biological Sciences (SIBS), a CAS research arm in biology, consists of the Institute Biochemistry and Cell Biology, the Institute of Neuroscience, the Institute of Materia Medica, the Institute of Physiology and Ecology, and the Institute for Nutritional Sciences. In response to the current trends of S&T globalization and the interdisciplinary development of the life sciences, it deems international cooperation amajor component of its effort to uplift the overall capacity of knowledge creation at SIBS.

  8. Cross-reactivity features of deoxynivalenol (DON)-targeted immunoaffinity columns aiming to achieve simultaneous analysis of DON and major conjugates in cereal samples

    Science.gov (United States)

    Gonçalves, Carlos; Stroka, Joerg

    2016-01-01

    ABSTRACT Immunoaffinity columns (IACs) are a well-established tool in the determination of regulated mycotoxins in food and feed commodities. However, they also have the potential to become attractive pre-concentration and clean-up materials for the determination of masked (also called modified) mycotoxins, which have been recognised as important contributors to the toxicological hazard deriving from fungal spoilage of goods. However, the information available in the literature concerning the cross-reactivity of DON-IACs against the major conjugates (DON-3-G, 15-AcDON and 3-AcDON) is incomplete and often contradictory. We have carried out a detailed characterisation of the cross-reactivity of the four main IACs brands against DON and its conjugates as well as an assessment of the competition among the analytes. Only one IAC enabled the simultaneous analysis of all relevant DON forms while two missed 15-AcDON and the fourth one missed DON-3-G and 3-AcDON. In the case of the multivalent IAC, the analytes modified at the C-3 position compete for the antibody binding with preference for 3-AcDON (less spatially hindered) while DON-3-G has the more-hindered access to the active sites. Taking into consideration the levels of DON conjugates existing in real samples, the cross-reactivity of one DON-IAC allows a quantitative analysis of all of these analytes. Important but rather neglected aspects such as the continuous supply of IACs with identical characteristics, and of columns which are strictly blank, are also addressed in this paper. PMID:27243664

  9. Cross-reactivity features of deoxynivalenol (DON)-targeted immunoaffinity columns aiming to achieve simultaneous analysis of DON and major conjugates in cereal samples.

    Science.gov (United States)

    Gonçalves, Carlos; Stroka, Joerg

    2016-06-01

    Immunoaffinity columns (IACs) are a well-established tool in the determination of regulated mycotoxins in food and feed commodities. However, they also have the potential to become attractive pre-concentration and clean-up materials for the determination of masked (also called modified) mycotoxins, which have been recognised as important contributors to the toxicological hazard deriving from fungal spoilage of goods. However, the information available in the literature concerning the cross-reactivity of DON-IACs against the major conjugates (DON-3-G, 15-AcDON and 3-AcDON) is incomplete and often contradictory. We have carried out a detailed characterisation of the cross-reactivity of the four main IACs brands against DON and its conjugates as well as an assessment of the competition among the analytes. Only one IAC enabled the simultaneous analysis of all relevant DON forms while two missed 15-AcDON and the fourth one missed DON-3-G and 3-AcDON. In the case of the multivalent IAC, the analytes modified at the C-3 position compete for the antibody binding with preference for 3-AcDON (less spatially hindered) while DON-3-G has the more-hindered access to the active sites. Taking into consideration the levels of DON conjugates existing in real samples, the cross-reactivity of one DON-IAC allows a quantitative analysis of all of these analytes. Important but rather neglected aspects such as the continuous supply of IACs with identical characteristics, and of columns which are strictly blank, are also addressed in this paper. PMID:27243664

  10. Tryptophan over-producing cell suspensions of Catharanthus roseus (L) G. Don and their up-scaling in stirred tank bioreactor: detection of a phenolic compound with antioxidant potential.

    Science.gov (United States)

    Verma, Priyanka; Mathur, Ajay K; Masood, Nusrat; Luqman, Suaib; Shanker, Karuna

    2013-02-01

    Five cell suspension lines of Catharanthus roseus resistant to 5-methyl tryptophan (5-MT; an analogue of tryptophan) were selected and characterized for growth, free tryptophan content and terpenoid indole alkaloid accumulation. These lines showed differential tolerance to analogue-induced growth inhibition by 30 to 70 mg/l 5-MT supplementation (LD(50) = 7-15 mg/l). Lines P40, D40, N30, D50 and P70 recorded growth indices (i.e. percent increment over the initial inoculum weight) of 840.9, 765.0, 643.9, 585.7 and 356.5 in the absence and, 656.7, 573.9, 705.8, 489.0 and 236.0 in the presence of 5-MT after 40 days of culture, respectively. A corresponding increment in the free tryptophan level ranging from 46.7 to 160.0 μg/g dry weight in the absence and 168.0 to 468.0 μg/g dry weight in the presence was noted in the variant lines. Higher tryptophan accumulation of 368.0 and 468.0 g/g dry weight in lines N30 and P40 in 5-MT presence also resulted in higher alkaloid accumulation (0.65 to 0.90 % dry weight) in them. High-performance liquid chromatography (HPLC) analysis of the crude alkaloid extracts of the selected lines did not show the presence of any pharmaceutically important monomeric or dimeric alkaloids except catharanthine in traces in the N30 line that was also unique in terms of a chlorophyllous green phenotype. The N30 line under optimized up-scaling conditions in a 7-l stirred tank bioreactor using Murashige and Skoog medium containing 2 mg/l α-naphthalene acetic acid and 0.2 mg/l kinetin attained 18-folds biomass accumulation within 8 weeks. Interestingly, the cell biomass yield was enhanced to 30-folds if 30 mg/l 5-MT was added in the bioreactor vessel one week prior to harvest. Crude alkaloid extract of the cells grown in shake flask and this bioreactor batch also showed the formation of yellow-coloured crystals which upon (1)HNMR and ESI-MS analysis indicated a phenolic identity. This crude alkaloid extract of bioreactor-harvested cells containing

  11. U.S. NAVY OFFICER ATTITUDES ON THE REPEAL OF "DON'T ASK, DON'T TELL"

    OpenAIRE

    Appleman, Ryan P.; McLaughlin, Peter S.

    2013-01-01

    See a presentation from this author for this work: http://hdl.handle.net/10945/39528 "Don't Ask, Don't Tell" (DADT) prohibited gays from serving openly in the military from December 1993 to September 2011. In February 1994, a survey of Navy officers was administered at the Naval Postgraduate School exploring attitudes toward DADT. This survey was re-administered in 1996, 1999, 2004, and 2010. The surveys revealed an increasing acceptance of gays in the Navy. The present study, conducted po...

  12. Results of sunflower breeding in resistance to broomrape on Don

    OpenAIRE

    Gorbachenko F.I.; Usatenko T.V.; Gorbachenko O.F.

    2011-01-01

    Results of long term research (1925-2009) on sunflower breeding in resistance to broomrape in Don Region are hereby presented. The most virulent population of Orobanche cummana Wallr. has been found in north-western part of Rostov region. Recommendations for reducing loss in sunflower yield caused by broomrape are given.

  13. Fjodor Bondartshuk dodelal "Tihhi Don" ottsa / Olga Saburova

    Index Scriptorium Estoniae

    Saburova, Olga

    2006-01-01

    Fjodor Bondartshuk monteeris kokku tema isa Sergei poolt Itaalias 13 aastat tagasi pooleli jäänud Mihhail Sholohhovi romaani järgi tehtud teleseriaali "Vaikne Don", mida hakkab Venemaa televisiooni 1. Kanal näitama 7. novembril

  14. "Tihhi Don" : Fjodor Bondartshuk zakontshil film svojego otsa / Darja Zavgorodnjaja

    Index Scriptorium Estoniae

    Zavgorodnjaja, Darja

    2006-01-01

    Fjodor Bondartshuk monteeris kokku tema isa Sergei poolt Itaalias 13 aastat tagasi pooleli jäänud Mihhail Sholohhovi romaani järgi tehtud teleseriaali "Vaikne Don", mida hakkab Venemaa televisiooni 1. Kanal näitama 7. novembril

  15. G. Don Taylor named the Charles O. Gordon Professor

    OpenAIRE

    Nystrom, Lynn A.

    2004-01-01

    G. Don Taylor, of Blacksburg, Va., professor and head of the Grado Department of Industrial and Systems Engineering in the College of Engineering at Virginia Tech, was named the Charles O. Gordon Professor of Industrial Engineering by the Virginia Tech Board of Visitors at its quarterly meeting Monday, Aug. 23.

  16. IDEA: An Interdisciplinary Unit Comparing "Don Quixote" to "Hamlet."

    Science.gov (United States)

    Harris, Mary J. G.

    2001-01-01

    Describes an idea for teaching language through content-based instruction in which a high school Spanish class studying a shortened abridged version of Cervantes'"Don Quixote" and an English class reading Shakespeare's "Hamlet," did a simple comparative analysis of the two texts. (Author/VWL)

  17. "Don Kihotõ" nazõvajut lutshshih / Boris Tuch

    Index Scriptorium Estoniae

    Tuch, Boris, 1946-

    2003-01-01

    Lõppenud festivali FIPRESCI auhinna sai türgi film "Kauge" ("Uzak") : režissöör Nuri Bilge Ceylan. Rahvusvaheline filmiklubide föderatsioon (FICC) andis oma auhinna Don Quijote Andrei Zvjagintsevi filmile "Tagasitulek" ("Vozvrashtshenije") ja eriauhinna hiinlase Chen Kaige filmile "Üheskoos" ("Han ni zai ylki")

  18. A phenolic glycoside from Fagopyrum dibotrys (D. Don) Hara

    Institute of Scientific and Technical Information of China (English)

    Zheng Zhong Bai; Xiao Hui Zhang; Li Jiang Xuan; Feng Kui Mo

    2007-01-01

    A new phenolic constituent along with five known compounds were isolated from Fagopyrum dibotrys (D.Don) Hara. The new compound was characterized as 1,3-dimethoxy-2-O-β-xylo-pyranosyl-5-O-β-glucopyranosyl-benzene, by spectroscopic analysis and enzymatic hydrolysis.

  19. Det litterære rum i Don Quixote

    DEFF Research Database (Denmark)

    Brink, Dennis Meyhoff

    Don Quixote kommer til verden i det måske mest afgørende øjeblik i den vestlige rumopfattelses historie - nemlig i det øjeblik, hvor forestillingen om, at vi lever i et uendeligt univers, erstatter den gammeleuropæiske forestilling om verdensrummet som et endeligt kosmos. Det nye verdensrum danner...

  20. Microfluidics made easy: A robust low-cost constant pressure flow controller for engineers and cell biologists.

    Science.gov (United States)

    Mavrogiannis, Nicholas; Ibo, Markela; Fu, Xiaotong; Crivellari, Francesca; Gagnon, Zachary

    2016-05-01

    Over the last decade, microfluidics has become increasingly popular in biology and bioengineering. While lab-on-a-chip fabrication costs have continued to decrease, the hardware required for delivering controllable fluid flows to the microfluidic devices themselves remains expensive and often cost prohibitive for researchers interested in starting a microfluidics project. Typically, microfluidic experiments require precise and tunable flow rates from a system that is simple to operate. While many labs use commercial platforms or syringe pumps, these solutions can cost thousands of dollars and can be cost prohibitive. Here, we present an inexpensive and easy-to-use constant pressure system for delivering flows to microfluidic devices. The controller costs less than half the price of a single syringe pump but can independently switch and deliver fluid through up to four separate fluidic inlets at known flow rates with significantly faster fluid response times. It is constructed of readily available pressure regulators, gauges, plastic connectors and adapters, and tubing. Flow rate is easily predicted and calibrated using hydraulic circuit analysis and capillary tubing resistors. Finally, we demonstrate the capabilities of the flow system by performing well-known microfluidic experiments for chemical gradient generation and emulsion droplet production. PMID:27279931

  1. L’utilité de la théorie du don The utility of gift theory Utilidad de la teoría del don

    OpenAIRE

    Norbert Alter

    2012-01-01

    Norbert Alter explique l’utilité et l’actualité de la théorie de Marcel Mauss pour penser la coopération en entreprise et l’engagement au travail. Il revient sur ses qualités heuristiques et sa portée critique nécessaires à mobiliser pour donner des clefs de compréhension et d’action au monde du travail. À cette contribution répond celle d’Alain Caillé, « Le paradigme du don face aux nouvelles réalités du monde du travail. Quelques remarques »Norbert Alter explains here the utility and topica...

  2. Pathway of deoxynivalenol-induced apoptosis in human colon carcinoma cells

    International Nuclear Information System (INIS)

    The mycotoxin, deoxynivalenol (DON), is generally detected in cereal grains and grain-based food products worldwide. Therefore, DON has numerous toxicological effects on animals and humans. The present investigation was conducted to determine the molecular aspects of DON toxicity on human colon carcinoma cells (HT 29). To this aim, we have monitored the effects of DON on (i) cell viability, (ii) Heat shock protein expressions as a parameter of protective and adaptive response, (iii) oxidative damage and (iv) cell death signalling pathway. Our results clearly showed that DON treatment inhibits cell proliferation, did not induce Hsp 70 protein expression and reactive oxygen species generation. We have also demonstrated that this toxin induced a DNA fragmentation followed by p53 and caspase-3 activations. Finally, our findings suggested that oxidative damage is not the major contributor to DON toxicity. This mycotoxin induces direct DNA lesions and could be considered by this fact as a genotoxic agent inducing cell death via an apoptotic process.

  3. CDC Vital Signs–Blood Pressure Medicines Don’t Work If People Don’t Take Them

    Centers for Disease Control (CDC) Podcasts

    2016-09-13

    Blood pressure medicines don’t work if people don’t take them. Learn how health care systems can work with patients to make taking medicines easier.  Created: 9/13/2016 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 9/13/2016.

  4. Pharmacognostical studies of leaves of Combretum albidum G. Don

    OpenAIRE

    Zalke, Ashish S.; Duraiswamy, B.; Gandagule, Upendra B.

    2013-01-01

    Background: Combretum albidum Don belonging to family Combretaceae is an unexplored medicinal plant in the Indian medicinal system. According to ethnobotanical information, the leaves are used in the treatment of peptic ulcer and its fruits are used in diarrhoea and dysentery. Stem bark is used in the treatment of jaundice and skin diseases. The problem encountered in standardisation of this medicinal plant is its identification by source. Materials and Methods: The pharmacognostical stud...

  5. Deoxynivalenol (DON) degradation and peroxidase enzyme activity in submerged fermentation

    OpenAIRE

    Jaqueline Garda-Buffon; Larine Kupski; Eliana Badiale-Furlong

    2011-01-01

    This work aims to evaluate deoxynivalenol degradation by Aspergillus oryzae and Rhizopus oryzae in a submerged fermentation system and to correlate it to the activity of oxydo-reductase enzymes. The submerged medium consisted of sterile distilled water contaminated with 50 μg of DON and 4 × 10(6) spore.mL-1 inoculum of Aspergillus oryzae and Rhizopus oryzae species, respectively in each experiment. Sampling was performed every 24 hours for monitoring the peroxidase specific activity, and ever...

  6. STATE EVALUATION OF WOODY PLANTS IN ROSTOV-ON-DON

    OpenAIRE

    Gudzenko E. O.

    2015-01-01

    There has been conducted the analysis of woody plants quantity in various types of stands in Rostov-on-Don, as well as the evaluation of their state and the rate of environmental resistance destabilization. It has been ascertained that the stands of almost all of the urban gardening objects are in a salvaged condition. There have been elaborated recommendations for enhancement of the stands inspected

  7. STATE EVALUATION OF WOODY PLANTS IN ROSTOV-ON-DON

    Directory of Open Access Journals (Sweden)

    Gudzenko E. O.

    2015-10-01

    Full Text Available There has been conducted the analysis of woody plants quantity in various types of stands in Rostov-on-Don, as well as the evaluation of their state and the rate of environmental resistance destabilization. It has been ascertained that the stands of almost all of the urban gardening objects are in a salvaged condition. There have been elaborated recommendations for enhancement of the stands inspected

  8. Catharanthus roseus (L.) G. Don - plant regeneration and alkaloids content

    OpenAIRE

    Mirosława Furmanowa; Hanna Olędzka; Joanna Józefowicz; Agnieszka Pietrosiuk

    2014-01-01

    We describe here a regeneration of plantlets of Catharanthus roseus (L.) G. Don from shoot tips and axillary buds. Shoot tips were excised from 7-day-old seedlings and were incubated in solid Nitsch and Nitsch (NN) medium supplemented with kinetin, benzyladenine (BA), indole-3-butyric acid (IBA) and β-indolylacetic acid (IAA) in various combinations. After two months in culture, regenerated rooted plantlets were cut and transferred to a new medium; the explants contained shoot tips or axillar...

  9. Épistémologie de la biologie et conceptualisation du vivant chez des futurs enseignants et biologistes

    Directory of Open Access Journals (Sweden)

    Simard Catherine

    2015-01-01

    Full Text Available L’ensemble des résultats présentés dans cet article relève d’une étude doctorale qui s’est intéressée à l’histoire de la biologie et aux grandes conceptions qui ont alimenté la construction des savoirs en biologie jusqu’à présent (Simard, 2015 [1]. À cet égard, un ensemble de conceptions du vivant a été sélectionné et circonscrit afin de développer un nouvel outil d’évaluation estimant la présence de l’une ou l’autre de ces conceptions chez les futurs enseignants et biologistes. Pour certaines conceptions s’avérant désuètes, la problématique est qu’elles se voient reconduites, encore aujourd’hui, dans la pensée scientifique. C’est alors en termes d’obstacle épistémologique historique à une conceptualisation contemporaine du vivant qu’elles sont appréhendées. Cette caractérisation de la conceptualisation du vivant et l’identification de conceptionsobstacles sont un passage nécessaire afin d’étudier, ultérieurement, les processus d’apprentissage et l’élaboration de séquences didactiques en les considérant au profit d’une compréhension actuelle du vivant. Enfin, est présentée brièvement l’influence positive que peut avoir une formation scientifique en biologie sur la conceptualisation du vivant et sur les conceptions-obstacles, qui se voient alors décliner*.

  10. Environmental radiation effects. A need to question old paradigms and to enhance collaboration between radiation biologists and radiation ecologists

    International Nuclear Information System (INIS)

    The radiological sciences are a real enigma- the maturity and depth of understanding concerning human dosimetry contrasts sharply with our shallow understanding about radiological effects to biota. The richness of the radiological sciences is apparent by looking at the refinements made to the fundamental unit used in human dosimetry. The radiological sciences have developed to where probabilistic risk factors can now be applied that predict specific deleterious effects to humans per unit dose. And yet, these same radiological sciences that have made such advances in human dosimetry, are primitive when effects to biota are concerned. There are no specialized units, no agreed upon weighting factors, no factors that account for distributions within an organism's body, and certainly no risk factors. There are no internationally agreed upon criteria or policies that explicitly address protection of the environment from ionizing radiation. There is not even agreement as to what endpoint should be measured to quantify an environmental effect. The bold aspect of the ICRP framework is the inclusion of sub-lethal effects (reduced reproductive success, scorable DNA damage) as endpoints. A major research consortium funded by the European Union, is also recommending that cytogenetic damage be used as an effect endpoint. The inclusion of sub-lethal endpoints begs for a linkage between molecular effects and those observed in individuals and populations. To do so, will require a strengthening of what has traditionally been separated disciplines of radiation biology and radiation ecology. The impacts of phenomena studied in the petri dishes of radiation biologists (such as genomic instability, adaptive response, and bystander effects) need to be explored and correlated to effects observed in whole organisms and populations, in collaboration with radiation ecologists. (M. Suetake)

  11. Lesbian, Gay, Bisexual, and Transgender (LGBT) Service Members: Life After Don't Ask, Don't Tell.

    Science.gov (United States)

    Goldbach, Jeremy T; Castro, Carl Andrew

    2016-06-01

    Lesbian, gay, and bisexual service members can serve openly in the military with the repeal of the Don't Ask, Don't Tell policy. The fate of transgender service members remains uncertain as the policy preventing them from serving in the military remains under review. The health care needs of these populations remain for the most part unknown, with total acceptance and integration in the military yet to be achieved. In this paper, we review the literature on the health care needs of lesbian, gay, bisexual, and transgender (LGBT) service members, relying heavily on what is known about LGBT civilian and veteran populations. Significant research gaps about the health care needs of LGBT service members are identified, along with recommendations for closing those gaps. In addition, recommendations for improving LGBT acceptance and integration within the military are provided. PMID:27086317

  12. KESALAHAN PENGGUNAAN PUNGTUASI DALAM SURAT DINAS DI SD FRATER DON BOSCO TARAKAN

    OpenAIRE

    Yohanis Tandi

    2011-01-01

    YOHANIS TANDI , 2011. Kesalahan Penggunaan Pungtuasi dalam Surat Dinas di SD Frater Don Bosko Tarakan. Skipsi Program Studi Pendidikan Bahasa, Sastra Indonesia dan Daerah FKIP Universitas Borneo Tarakan, pembimbing: (1) Zulkifli, M.Pd. (II) Drs. Petrus Tenabean. Rumusan masalah dalam penelitian ini, yaitu penggunaan pungtuasi dalam surat dinas di SD Frater Don Bosko tarakan. Penelitian ini bertujuan untuk mendeskripsikan pemakaian pungtuasi di dalam surat dinas di SD Frarter Don Bosko Taraka...

  13. The Authors of the Ingenious Hidalgo Don Quixote of La Mancha

    OpenAIRE

    Branka Kalenić Ramšak

    2008-01-01

    Even today literary criticism still considers the novel Don Quixote the first modern European novel because it fundamentally changes both the concept of literary creation and the findings regarding literary reception. Miguel de Cervantes Saavedra published the first part of his chivalric novel in 1605 in Madrid with the title El ingenioso hidalgo don Quijote de la Mancha. He published the second part, titled El ingenioso caballero don Quijote de la Mancha, ten years later (i.e., in 1615), aga...

  14. Mechanistic modeling confronts the complexity of molecular cell biology

    OpenAIRE

    Phair, Robert D.

    2014-01-01

    Mechanistic modeling has the potential to transform how cell biologists contend with the inescapable complexity of modern biology. I am a physiologist–electrical engineer–systems biologist who has been working at the level of cell biology for the past 24 years. This perspective aims 1) to convey why we build models, 2) to enumerate the major approaches to modeling and their philosophical differences, 3) to address some recurrent concerns raised by experimentalists, and then 4) to imagine a fu...

  15. L’utilité de la théorie du don

    OpenAIRE

    Alter, Norbert

    2012-01-01

    Norbert Alter explique l’utilité et l’actualité de la théorie de Marcel Mauss pour penser la coopération en entreprise et l’engagement au travail. Il revient sur ses qualités heuristiques et sa portée critique nécessaires à mobiliser pour donner des clefs de compréhension et d’action au monde du travail. À cette contribution répond celle d’Alain Caillé, « Le paradigme du don face aux nouvelles réalités du monde du travail. Quelques remarques »

  16. Don Quixote Pond: A Small Scale Model of Weathering and Salt Accumulation

    Science.gov (United States)

    Englert, P.; Bishop, J. L.; Patel, S. N.; Gibson, E. K.; Koeberl, C.

    2015-01-01

    The formation of Don Quixote Pond in the North Fork of Wright Valley, Antarctica, is a model for unique terrestrial calcium, chlorine, and sulfate weathering, accumulation, and distribution processes. The formation of Don Quixote Pond by simple shallow and deep groundwater contrasts more complex models for Don Juan Pond in the South Fork of Wright Valley. Our study intends to understand the formation of Don Quixote Pond as unique terrestrial processes and as a model for Ca, C1, and S weathering and distribution on Mars.

  17. A focus on parietal cells as a renewing cell population

    Institute of Scientific and Technical Information of China (English)

    Sherif; M; Karam

    2010-01-01

    The fact that the acidsecreting parietal cells undergo continuous renewal has been ignored by many gastroenterologists and cell biologists. In the past, it was thought that these cells were static. However, by using 3Hthymidine radioautography in combination with electron microscopy, it was possible to demonstrate that parietal cells belong to a continuously renewing epithelial cell lineage. In the gastric glands, stem cells anchored in the isthmus region are responsible for the production of parietal cells...

  18. Cytotoxicity and metabolic stress induced by deoxynivalenol in the porcine intestinal IPEC-J2 cell line.

    Science.gov (United States)

    Awad, W A; Aschenbach, J R; Zentek, J

    2012-08-01

    The digestive tract is a target for the Fusarium toxin deoxynivalenol (DON), a major cereal grain contaminant of animal and public health concern. Toxic effects of DON range from diarrhoea, vomiting and gastrointestinal inflammation to necrosis of several tissues. Following ingestion of contaminated food or feed, intestinal epithelial cells are exposed to a high concentration of ingested DON, potentially affecting intestinal functions. Pigs are considered to be the species most sensitive to DON toxicity. However, only few studies directly evaluated DON effects on porcine intestinal epithelial cells. Therefore, we used the porcine intestinal cell line (IPEC-J2) to assess short-term effects of DON on functional characteristics of the intestinal epithelial cells. The cytotoxic effect of DON on IPEC-J2 cells was evaluated by measuring the count of living cells and the activity of lactate dehydrogenase (LDH) released in the culture media at a DON concentration range from 0, 0.5, 2.5 and 10 μm. We demonstrated that DON at concentrations of 2.5 and 10 μm decreased significantly (p mycotoxin, DON, increased LDH release into the culture medium compared with the control value. The alterations of LDH showed a good agreement with the decrease in cell count. Deoxynivalenol decreased the l-lactate concentration in the fluid supernatant of IPEC-J2 cells at 2.5 μm (p < 0.05) with a maximal effect at 10 μm of DON. To determine whether the altered lactate production may be linked to alterations of energy balance, we measured cellular ATP levels in IPEC-J2 cells. A significant decrease in ATP levels was seen at 48 h in a dose-dependent manner. It could be demonstrated that DON has a distinct cytotoxic effect on IPEC-J2 cells. PMID:21793942

  19. Vindogentianine, a hypoglycemic alkaloid from Catharanthus roseus (L.) G. Don (Apocynaceae).

    Science.gov (United States)

    Tiong, Soon Huat; Looi, Chung Yeng; Arya, Aditya; Wong, Won Fen; Hazni, Hazrina; Mustafa, Mohd Rais; Awang, Khalijah

    2015-04-01

    Vindogentianine, a new indole alkaloid together with six known alkaloids, vindoline, vindolidine, vindolicine, vindolinine, perivine and serpentine were isolated from leaf extract (DA) of Catharanthus roseus (L.) G. Don. Their structures were elucidated by spectroscopic methods; NMR, MS, UV and IR. Vindogentianine is a dimer containing a vindoline moiety coupled to a gentianine moiety. After 24h incubation, vindogentianine exhibited no cytotoxic effect in C2C12 mouse myoblast and β-TC6 mouse pancreatic cells (IC50>50μg/mL). Real-time cell proliferation monitoring also indicated vindogentianine had little or no effect on C2C12 mouse myoblast cell growth at the highest dose tested (200μg/mL), without inducing cell death. Vindogentianine exhibited potential hypoglycemic activity in β-TC6 and C2C12 cells by inducing higher glucose uptake and significant in vitro PTP-1B inhibition. However, in vitro α-amylase and α-glucosidase inhibition assay showed low inhibition under treatment of vindogentianine. This suggests that hypoglycemic activity of vindogentianine may be due to the enhancement of glucose uptake and PTP-1B inhibition, implying its therapeutic potential against type 2 diabetes. PMID:25665941

  20. Ascaridia galli infection affects pullets differently when feed is contaminated with the Fusarium toxin deoxynivalenol (DON).

    Science.gov (United States)

    Dänicke, S; Beineke, A; Rautenschlein, Silke; Valenta, Hana; Kersten, Susanne; Gauly, M

    2013-12-01

    The Fusarium mycotoxin deoxynivalenol (DON) is a common contaminant of cereal grains used as animal feed. DON is known for its cytotoxic and anti-proliferative properties and might adversely affect the health of poultry. The prevalence of the intestinal parasitizing roundworm Ascaridia galli is higher in outdoor housing systems and has been associated with maldigestion and malabsorption. It was hypothesized that ingested DON might not only affect the pullet itself but could also act on the nematode parasitizing in the ingesta. To examine these interactions between A. galli infection and DON contamination of feed 4 groups of 9 pullets in each were tested; non-infected groups were fed either an uncontaminated control (CON-) or a Fusarium toxin contaminated and mainly DON-containing diet (FUS-), and the corresponding A. galli inoculated groups were fed accordingly (CON+, FUS+). A. galli infection significantly reduced the jejunal villi height and increased the thickness of the tunica muscularis with the effect being more pronounced when the DON-containing diet was fed (Group FUS+). Only in this group significantly increased weights of jejunal and ileal tissues and of livers were noticed. Moreover, DON was detected in plasma of the pullets at higher frequencies when they were infected suggesting a facilitated absorption of DON. Group FUS+ was characterized by a significantly higher excretion of A. galli eggs and a concomitant lower proportion of pullets with detectable antibodies against a somatic antigen of A. galli while worm burden and worm characteristics were not affected by diet. Other effects of feeding the FUS diet to the infected pullets included an increased mass per length of male worms. In conclusion, infection of pullets with A. galli might increase the susceptibility towards DON as indicated by an increased DON absorption rate and a compromised antibody formation. The effects of DON on fecundity and worm morphology require further examination. PMID

  1. Don, Betty and Jackie Kennedy: On Mad Men and Periodisation

    Directory of Open Access Journals (Sweden)

    Prudence Black

    2012-09-01

    Full Text Available Why is it that we watch Mad Men and think it represents a period? Flashes of patterned wallpaper, whiskey neat, babies born that are never mentioned, contact lining for kitchen drawers, Ayn Rand, polaroids, skinny ties, Hilton hotels, Walter Cronkite, and a time when Don Draper can ask ‘What do women want?’ and dry old Roger Sterling can reply ‘Who Cares?’ This essay explores the embrace of period detail in Mad Men finding it to be both loving and fetishistic, and belonging, like all period film, to the politics of the present.

  2. Digitaalisen markkinointiviestinnän suunnittelu Facebookissa: Ravintola Don Corleone

    OpenAIRE

    Dondych, Fanny

    2016-01-01

    Tämän opinnäytetyön tavoitteena oli kehittää viestintäsuunnitelma ravintolalle Don Corleone digi-taalisesta markkinointiviestinnästä Facebookissa. Työn tarkoituksena oli tehostaa ravintolan mark-kinointiviestintää Facebookissa, mikä johtaisi asiakkaiden lisääntymiseen ja sitä kautta liiketoimin-nan kasvuun. Sosiaalisen median palveluista viestintäkanavaksi valittiin Facebook toimeksiantajan toiveesta sekä Facebookin suuren suosion ja soveltuvuuden vuoksi. Facebookissa on valmiiksi pal-jon eri...

  3. Entre burlas y veras: Don Quijote como loco-cuerdo

    OpenAIRE

    Gallardo Saborido, Emilio J.

    2009-01-01

    Este artículo indaga en la configuración plural y ambivalente de la figura de Don Quijote, centrándose específicamente en la caracterización de este personaje como loco-cuerdo. Así pues, se consideran varios mecanismos con los que Cervantes jugó para tal fin: desde el uso de la verosimilitud y la parodia, hasta el recurso a modelos médicos propios de su época, pasando por revisiones de arquetipos prestigiosos, como el del caballero erasmista. Las conclusiones obtenidas inciden en la contrapos...

  4. Comunidades de afectados, procomún y don expandido

    OpenAIRE

    Lafuente, Antonio; Corsín Jiménez, Alberto

    2010-01-01

    El don existe. Existe, pero como tantas cosas en nuestro mundo, incluso muy importantes, parece invisible. Allí siempre habrá una mujer cuidando de un bebé, un enfermo o un anciano. En los alrededores, cualquiera que sea la dirección a donde apuntemos, habrá alguien usando la lengua, respirando aire, jugando en la calle o protestando por el ruido. Y conste que podríamos subir el tono y mencionar todo lo que tenga que ver con añorar justicia, gozar paisajes, reclamar salud o disfrutar el si...

  5. SAT-Based Complete Don't-Care Computation for Network Optimization

    CERN Document Server

    Mishchenko, Alan

    2011-01-01

    This paper describes an improved approach to Boolean network optimization using internal don't-cares. The improvements concern the type of don't-cares computed, their scope, and the computation method. Instead of the traditionally used compatible observability don't-cares (CODCs), we introduce and justify the use of complete don't-cares (CDC). To ensure the robustness of the don't-care computation for very large industrial networks, a optional windowing scheme is implemented that computes substantial subsets of the CDCs in reasonable time. Finally, we give a SAT-based don't-care computation algorithm that is more efficient than BDD-based algorithms. Experimental results confirm that these improvements work well in practice. Complete don't-cares allow for a reduction in the number of literals compared to the CODCs. Windowing guarantees robustness, even for very large benchmarks on which previous methods could not be applied. SAT reduces the runtime and enhances robustness, making don't-cares affordable for a v...

  6. Research Advancement on Biosynthesis and Biodegradation of Deoxynivalenol (DON)%呕吐毒素(DON)生物合成和降解研究进展

    Institute of Scientific and Technical Information of China (English)

    曹慧英; 伍松陵; 孙长坡

    2013-01-01

    Vomitoxin,also known as deoxynivalenol (DON),is a kind of secondary metabolites produced by certain Fusarium species;it is one of the common mycotoxins which polluted the food.With the frequent occurrence of climate disasters domestically,the trend of DON pollution exacerbates,so prevention and treatment of DON contamination are imminent.This peper covers the physical and chemical properties of DON,analysis of the DON biosynthetic pathways based on its chemical structure and genes,and summarizes biodegradation of DON by microorganism,speculates possible sites of degradation,degradation pathways and products.Based on a comprehensive understanding of DON,it aims to inspire to seek safe,efficient and cost effective biodegradation pathways using biotechnology,finally to ensure grain and food safety and protect health of consumers.%呕吐毒素(Vomitoxin),即脱氧雪腐镰刀菌烯醇(deoxynivalenol,DON),是镰刀菌产生的次生代谢物质,是污染粮食的主要真菌毒素.随着我国灾害气候的频频发生,DON的污染程度呈现加剧趋势,因此DON污染的控制迫在眉睫.本文阐述了DON的物理化学特性,结合化学结构和基因遗传分析了DON的生物合成途径,并综述了微生物对DON的生物降解研究,推测了DON可能的作用位点、降解途径及降解产物,以此启发利用生物技术,探求安全、高效,低成本的DON生物降解途径,确保粮食和食品安全、保护消费者健康.

  7. Blood Pressure Medicines Don’t Work If People Don’t Take Them PSA (:60)

    Centers for Disease Control (CDC) Podcasts

    2016-09-13

    This 60 second public service announcement is based on the September 2016 CDC Vital Signs report. Blood pressure medicines don’t work if people don’t take them. Learn how health care systems can work with patients to make taking medicines easier.  Created: 9/13/2016 by National Center for Chronic Disease Prevention and Health Promotion (NCCDPHP).   Date Released: 9/13/2016.

  8. Dual Effects Exerted in Vitro by Micromolar Concentrations of Deoxynivalenol on Undifferentiated Caco-2 Cells

    Science.gov (United States)

    Manda, Gina; Mocanu, Mihaela Andreea; Marin, Daniela Eliza; Taranu, Ionelia

    2015-01-01

    Contamination of crops used for food and feed production with Fusarium mycotoxins, such as deoxynivalenol (DON), raise important health and economic issues all along the food chain. Acute exposure to high DON concentrations can alter the intestinal barrier, while chronic exposure to lower doses may exert more subtle effects on signal transduction pathways, leading to disturbances in cellular homeostasis. Using real-time cellular impedance measurements, we studied the effects exerted in vitro by low concentrations of DON (0.37–1.50 μM), relevant for mycotoxin-contaminated food, on the proliferation of undifferentiated Caco-2 cells presenting a tumorigenic phenotype. A 1.5 μM concentration of DON maintained cell adherence of non-proliferating Caco-2 cells, whilst arresting the growth of actively proliferating cells compared with control Caco-2 cells in vitro. At 0.37 μM, DON enhanced Caco-2 cell metabolism, thereby triggering a moderate increase in cell proliferation. The results of the current study suggested that low concentrations of DON commonly detected in food may either limit or sustain the proliferation of colon cancer cells, depending on their proliferation status and on DON concentration. Soluble factors released by Lactobacillus strains can partially counteract the inhibitory action of DON on actively proliferating colon cancer cells. The study also emphasized that real-time cellular impedance measurements were a valuable tool for investigating the dynamics of cellular responses to xenobiotics. PMID:25690693

  9. Lidia Sedda, Don Chisciotte nella Cultura russa (1720-1928

    Directory of Open Access Journals (Sweden)

    Francesca Di Tonno

    2013-05-01

    Full Text Available Tracciare i percorsi, a volte limpidi altre volte estremamente complessi, attraverso i quali il Don Chisciotte di Miguel de Cervantes è penetrato nel mondo russo significa ripercorrere la storia di una cultura, quella russa, perennemente in bilico tra Occidente e Oriente. E infatti, la studiosa Lidia Sedda già nell’introduzione al suo saggio Don Chisciotte nella Cultura russa (1720-1928, ricorda come  «l’Europa, sin dai tempi di Pietro il Grande, ha svolto una parte importante nell’indirizzare le riflessioni della classe dirigente e dell’intelligencija russa» (Sedda 2010: 9. Ma al tempo stesso il rapporto tra Russia ed Europa si è «sempre configurato come un rapporto critico, per sua natura dialogico» (ibid.: 9 e  «il campo privilegiato nel quale si è svolto tale rapporto è stato prevalentemente quello letterario» (ibid.: 9. Ecco che allora l’autrice ripercorre con grande acribia filologica le dinamiche di ricezione del capolavoro di Cervantes nella Russia già seicentesca e poi man mano nel periodo che va dal XVIII al XX secolo.

  10. 黑暗和光照对长春花培养细胞生长和生理生化特性的影响%The Effects of Dark and Light on Growth and Physiological and Biochemical Characteristics in Cell Culture of Catharanthus roseus(L.)G.Don

    Institute of Scientific and Technical Information of China (English)

    郭胜娟; 刘树楠; 冯玲玲; 周吉源

    2004-01-01

    以长春花(Catharanthus roseus (L.)G.Don)幼嫩叶片为外植体,在MS+NAA 0.5 mg/L+2,4-D 0.5 mg/L+6 BA 2.0 mg/L培养基上诱导形成愈伤组织,愈伤组织置于不同条件下培养.结果表明,黑暗和光照下,培养细胞的生长周期为27 d;过氧化物酶(POD)活性和超氧化物歧化酶(SOD)活性均出现2个峰值.对长春花总生物碱含量和总产量亦做了研究,结果发现最佳的收获时期为第27 d.

  11. Don DeLillo’s Mapping of the City

    Directory of Open Access Journals (Sweden)

    Stipe Grgas

    2005-06-01

    Full Text Available Taking as his point of departure the immense significance the city has for understanding the present moment and the special relationship the city has had with the novel, the author gives a reading of Don DeLillo and the way his work has engaged the city of New York. Focusing upon his last two novels, Underworld and Cosmopolis, the author describes how these two novels narrate the transformations the American city has undergone during the second part of the twentieth century. The bulk of his analysis deals with the function the Prologue flashback of the Bronx has in the earlier novel and the transformed city of late capitalism in his last text. The author concludes his reading by pointing out how DeLillo’s novels not only provide fictional accounts of what has occurred in the urban sphere but how they provide evidence of the difficulty of representing the contemporary world and how they foreground urgent political considerations.

  12. Studies on phytosterol content of Parkia roxburgii G. Don.

    Directory of Open Access Journals (Sweden)

    DJADJAT TISNADJAJA

    2006-01-01

    Full Text Available Kedawung (Parkia roxburgii G. Don. is one of plants that originally could be found in almost all parts of Java Island. Due to lack of attention, at present situation this plant could be categorized as an endangered species. This plant species distributed widely in Africa. In several African countries, this plant has an important position either as food resource or alternative medicine. In Indonesia, traditionally kedawung tree often use as medicine, especially as part of “jamu gendong” formulation. This work was focused on the study of phytosterol content and it distribution. The research result show that almost all parts of kedawung tree have a significant content of phytosterol, which is dominated by beta-sitosterol. The highest beta-sitosterol content was founded in the stem of leaf (35.24% (w/w and pod (29.67% (w/w.

  13. Scientific Communication for Positive Action: Do's and Don'ts

    Science.gov (United States)

    Sanquini, A.; Wood, M. M.

    2015-12-01

    Natural hazard presentations often highlight disasters that may ensue from natural processes when mitigation or preparedness actions are not taken. Examples include images of raging fires, collapsed buildings, and flooded urban areas. Research has shown that this makes presentations more interesting and more memorable. Such images are the stock and trade of disaster reporting by the media. Unfortunately, it may also trigger avoidance and denial in the audience, resulting in preparedness reduction; the opposite effect of what may have been intended by the speaker. Recent social research has provided insight into a better approach. The theory of communicating actionable risk posits that people will take action against hazards when they know what to do, think it would work, and know someone who did it. This approach was recently applied in an intervention designed to motivate earthquake-resistant construction in Kathmandu Valley, Nepal. Social theory further suggests that a tendency towards action is strengthened by hearing a consistent message over time, and by providing the audience with an appropriate opportunity to seek out relevant information. This presentation shows how, by taking this transdisciplinary step, scientists can make small changes in their hazard communication, thereby acting as positive influencers of change. A summary of "do's" and "don'ts" is given for reference. 1. Show examples of what to do. 2. Show effectiveness of actions. 3. Give sense of knowing someone who did it. 4. Deliver consistent message, repeatedly. 5. Give opportunity for more information. DON'T: 1. Make the disaster the star. 2. Contradict other communications.

  14. Anatomical characters of the phyllode and stem of Acacia podalyriifolia A. Cunn. ex G. Don (Fabaceae

    Directory of Open Access Journals (Sweden)

    M.R. Duarte

    2005-03-01

    Full Text Available The Acacia genus has presented various secondary metabolites, such as tannins, flavonoids, alkaloids and gums. Preparations from different species have been applied for diabetes, gastrointestinal disorders and inflammatory diseases in the traditional medicine and have demonstrated cytotoxic, antimicrobial and antiparasitic activities. Acacia podalyriifolia A. Cunn. ex G. Don (Fabaceae is a small wood, indigenous to Australia and cultivated worldwide for its ornamental feature. This work aimed to characterize the anatomy of the phyllode and stem, in order to contribute to the species identification. The botanical material was fixed, sectioned and prepared according to usual light and scanning microtechniques. The epidermal cells, in surface view, are polygonal and coated with striate and thick cuticle, and filaments of epicuticular wax. Paracytic stomata and unicellular non-glandular trichomes are seen. Palisade and ground parenchymas, and minor collateral bundles with xylem directed alternately to upper and lower sides occur in the blade. The midrib shows two collateral bundles facing each other. The stem, in incipient secondary growth, exhibits epidermis, annular collenchyma, sclerenchymatic sheath and collateral vascular organization. Cells containing phenolic compounds and prisms of calcium oxalate are observed.

  15. Don Edwards San Francisco Bay National Wildlife Refuge: Annual Narrative Report: Calendar Year 2000

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This annual narrative report for Don Edwards San Francisco Bay NWR, Antioch Dunes NWR, Farallon NWR, San Pablo Bay NWR, Ellicot Slough NWR, Salinas River NWR, and...

  16. Regional economic impacts of current and proposed management alternatives for Don Edwards National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report first presents a description of the local community and economy near the Don Edwards San Francisco Bay NWR. Next, the report describes methods used to...

  17. Botulism protocols for the Don Edwards San Francisco Bay National Wildlife Refuge

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This plan gives a basic description of what avian botulism is and the locations on the Don Edwards San Francisco Bay National Wildlife Refuge where botulism...

  18. 4 in 10 Popular Sunscreens Don't Meet Sun Safety Standards

    Science.gov (United States)

    ... html 4 in 10 Popular Sunscreens Don't Meet Sun Safety Standards: Study Biggest problem with products ... products sold in the United States fail to meet basic sun safety guidelines, new research shows. The ...

  19. The Trail Inventory of Don Edwards San Francisco Bay National Wildlife Refuge [Cycle 1

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The purpose of this report is to create a baseline inventory of all nonmotorized trails on Don Edwards San Francisco Bay National Wildlife Refuge. Trails in this...

  20. Pilot Fullerton dons EES anti-gravity suit lower torso on middeck

    Science.gov (United States)

    1982-01-01

    Pilot Fullerton dons ejection escape suit (EES) anti-gravity (anti-g) suit lower torso on forward port side middeck above potable water tank. Anti-g suit is an olive drab inner garment that complements EES.

  1. Parents Often Don't Get Rid of Leftover Prescription Opioids

    Science.gov (United States)

    ... nlm.nih.gov/medlineplus/news/fullstory_158859.html Parents Often Don't Get Rid of Leftover Prescription ... Percocet, for surgery or illness, about half of parents say they keep the leftover medicine on hand. " ...

  2. Don't Blame the Media for Teen Sex, Study Says

    Science.gov (United States)

    ... 159740.html Don't Blame the Media for Teen Sex, Study Says Parents and peers have far ... television and other media have little effect on teens' sexual behavior, according to a study that challenges ...

  3. Crash Risk Soars When Truck Drivers Don't Treat Sleep Apnea

    Science.gov (United States)

    ... nlm.nih.gov/medlineplus/news/fullstory_157882.html Crash Risk Soars When Truck Drivers Don't Treat ... their treatment program are much more likely to crash, a new study finds. "The most surprising result ...

  4. Mixing regime as a key factor to determine DON formation in drinking water biological treatment.

    Science.gov (United States)

    Lu, Changqing; Li, Shuai; Gong, Song; Yuan, Shoujun; Yu, Xin

    2015-11-01

    Dissolved organic nitrogen (DON) can act as precursor of nitrogenous disinfection by-products formed during chlorination disinfection. The performances of biological fluidized bed (continuous stirred tank reactor, CSTR) and bio-ceramic filters (plug flow reactor, PFR) were compared in this study to investigate the influence of mixing regime on DON formation in drinking water treatment. In the shared influent, DON ranged from 0.71mgL(-1) to 1.20mgL(-1). The two biological fluidized bed reactors, named BFB1 (mechanical stirring) and BFB2 (air agitation), contained 0.12 and 0.19mgL(-1) DON in their effluents, respectively. Meanwhile, the bio-ceramic reactors, labeled as BCF1 (no aeration) and BCF2 (with aeration), had 1.02 and 0.81mgL(-1) DON in their effluents, respectively. Comparative results showed that the CSTR mixing regime significantly reduced DON formation. This particular reduction was further investigated in this study. The viable/total microbial biomass was determined with propidium monoazide quantitative polymerase chain reaction (PMA-qPCR) and qPCR, respectively. The results of the investigation demonstrated that the microbes in BFB2 had higher viability than those in BCF2. The viable bacteria decreased more sharply than the total bacteria along the media depth in BCF2, and DON in BCF2 accumulated in the deeper media. These phenomena suggested that mixing regime determined DON formation by influencing the distribution of viable, total biomass, and ratio of viable biomass to total biomass. PMID:25585870

  5. The discovery of cometary activity in near-earth asteroid (3552) don quixote

    OpenAIRE

    Mommert, Michael; Hora, Joseph L.; Harris, Alan W.; Reach, William T.; Emery, Joshua P.; Thomas, Cristina A.; Mueller, Michael; Cruikshank, Dale P.; Trilling, David E.; Delbo', Marco; Smith, Howard A.

    2014-01-01

    The near-Earth object (NEO) population, which mainly consists of fragments from collisions between asteroids in the main asteroid belt, is thought to include contributions from short-period comets as well. One of the most promising NEO candidates for a cometary origin is near-Earth asteroid (3552) Don Quixote, which has never been reported to show activity. Here we present the discovery of cometary activity in Don Quixote based on thermal–infrared observations made with the Spitzer S...

  6. FREE RADICAL SCAVENGING ACTIVITY AND HPLC ANALYSIS OF ARAUCARIA CUNNINGHAMII AITON EX D. DON LEAF EXTRACT

    OpenAIRE

    Vandana Gautam; Saroj Arora; Dhriti Kapoor; Renu Bhardwaj

    2014-01-01

    Background: Several diseases are caused in the body due to oxidative stress of free radicals. The objectives of the present study were to investigate the antioxidant activity of Araucaria cunninghamii Aiton ex D. Don (Araucariaceae) leaf extract. The dried leaves of Araucaria cunninghamii Aiton ex D. Don (Araucariaceae) were extracted with 80% methanol. The antioxidant activity of the extract was predicted through in vitro model systems such as 1,1-diphenyl-2-picryl hydrazyl (DPPH), reducing ...

  7. Stability of DON and OTA during the breadmaking process and determination of process and performance criteria

    OpenAIRE

    Vidal, A.; Morales-Valle, H.; Sanchis, V.; Ramos, A. J.; Marín, S.

    2014-01-01

    The fate of deoxynivalenol (DON) and ochratoxin A (OTA) during the breadmaking process was studied. In particular, toxin content was analysed in mixed baking ingredients before kneading, after fermentation and proofing, and finally after baking. Fermentation and proofing were carried out at 30 C for 1 h, while baking was performed at different temperature levels (from 170 to 210 C) and baking times from 45 to 135 min, in a full factorial design. DON increased from unkneaded mix to fermented d...

  8. MENINGKATKAN KEMAMPUAN MEMBACA SISWA DENGAN MENGGUNAKAN MEDIA GAMBAR KELAS ISD FRATER DON BOSCO TARAKAN

    OpenAIRE

    Yuliana Kaha

    2011-01-01

    Yuliana Kaha, 2011, Meningkatkan Kemampun Membaca Siswa Dengan Menggunakan Media Gambar Kelas 1 SD Frater Don Bosco Tarakan Tahun Pembelajaran 2010/ 2011. Skripsi Jurusan Pendidikan Guru Sekolah Dasar Universitas Borneo Tarakan, Pembimbing: (1) Sungkono,S.Pd,MA(2) Drs. Herdiansyah, M.Si. Skripsi ini berjudul Meningkatkan Kemampuan Membaca Siswa Dengan Menggunakan Media Gambar Kelas I SD Frater Don Bosco Tarakan Tahun pembelajaran 2010/ 2011. Skripsi ini merupakan hasil karya akademis untuk Me...

  9. Buy, don't build -- What does that mean for a software developer?

    International Nuclear Information System (INIS)

    The buzz phrase of the 1990's for the petroleum software industry has become ''buy, don't build.'' For an end user in an oil company, this generally means acquiring application software rather than developing it internally. The concept of buy, don't build can also apply for a software developer. Purchasing software toolkit components can expedite the development of an application as well as reduce future support requirements

  10. Antidiabetic and Antioxidant Properties of Alkaloids from Catharanthus roseus (L.) G. Don

    OpenAIRE

    Won Fen Wong; Shiau-Chuen Cheah; Mohd Rais Mustafa; Mohammadjavad Paydar; Aditya Arya; Soon Huat Tiong; Chung Yeng Looi; Khalijah Awang; Hazrina Hazni

    2013-01-01

    Catharanthus roseus (L.) G. Don is a herbal plant traditionally used by local populations in India, South Africa, China and Malaysia to treat diabetes. The present study reports the in vitro antioxidant and antidiabetic activities of the major alkaloids isolated from Catharanthus roseus (L.) G. Don leaves extract. Four alkaloids—vindoline I, vindolidine II, vindolicine III and vindolinine IV—were isolated and identified from the dichloromethane extract (DE) of this plant’s leaves. DE and comp...

  11. ‘Early Psychosis’ as a Mirror of Biologist Controversies in Post-WarGerman, Anglo-Saxon and Soviet Psychiatry

    Directory of Open Access Journals (Sweden)

    Lara eRzesnitzek

    2013-07-01

    Full Text Available The English term ‘early psychosis’ was coined in the 1930s to refer to feelings of irritability, loss of concentration, hypochondriac ideas, moodiness and lassitude that were seen to precede the onset of clear-cut hallucinations and delusions. The history of thinking about ‘early psychosis’ under names such as ‘latent’, ‘masked’, ‘mild’, ‘simple’ or ‘sluggish’ schizophrenia before World War II and afterwards on the different sides of the Wall and the Iron Curtain reveals ‘early psychosis’ as a mirror of quite aged international biologist controversies that are still alive today and to the same extent as they are misunderstood, are influential in their implications in today’s psychiatry.

  12. Demistifikasi Tokoh Don Juan dalam La Nuit De Valognes Karya Eric-Emmanuel Schmitt

    Directory of Open Access Journals (Sweden)

    Talha Bachmid

    2006-10-01

    Full Text Available Don Juan is a myth about a seducer, a man with many love adventures, a women’s heat conqueror, and many other predicates. Don Juan as a myth is well-known world wide over, appearing in about 50 writings such as novels, plays, operas. But a French writer Eric-Emannuel Schmitt proposes a new figure of Don Juan in his play La Nuit de Valognes (Night in Valognes: the seducer is judged by five victims and convicted to marry one of his victims in order to stop his adventures. Surprisingly, Don Juan accepts this verdict without conditions. This sudden change becomes the theme of the play: the demythification of Don Juan. A flash back scene shows the beginning of this change and the interpretations that might arise from here. One possible interpretation is proposed and discussed in this paper: Don Juan found the way to a purer love, which is the union with God, and decides to begin his journey. This play can thus be categorised as a sufi text.

  13. Consumption as Sign in Don DeLillo’s Cosmopolis

    Directory of Open Access Journals (Sweden)

    Seyyede Fateme Mirbabazade

    2013-11-01

    Full Text Available As one of the foremost American postmodern authors, Don DeLillo tends to focus on construction of postmodern subject’s consciousness through media, technology and consumption. Nevertheless, despite many affinities between Baudrillard and DeLillo, many researches which have been done on his works have failed to theoretically analyze them from Baudrillardian perspective. Although there is affinity between Baudrillard and DeLillo, there is dissimilarity as well. While Baudrillard believes that that there is no escape for individual from the ideology of the system, DeLillo claims that there is hope for change and revolution for the individuals by their action in society, particularly by the means of art, to be more  than a cog in the machine of ideology of system. Baudrillard, as one of the most influential postmodern theorists, defines consumption as organized control of sign and one of the many ways any subject is assimilated in any given society. For Baudrillard the logic of consumption is based on social differentiation. By the logic of social differentiation, individuals distinguish themselves and obtain social prestige and standing through the purchase and use of consumer goods. In his theorization about the concept of need and value, Baudrillard finds Marx’s logics of use value (utility and functionality and exchange value (economic value inadequate to include the whole function of an object and adds the logic of sign value. Baudrillard asserts that in consumption economic exchange value (money is transformed into sign exchange value (prestige, etc.; yet this functioning is maintained by the use value. The only way out of this dilemma, Baudrillard claims, is to return to symbolic exchange which is neither a conception nor an agency, but an act of exchange and a social relation and the only approach to real human communication and understanding. Therefore this research attempts to highlights those affinities in DeLillo’s first post-9

  14. Study on the Induction of Polyploid Bulb of Fritillaria Cirrhosa D. Don%卷叶贝母多倍体鳞茎诱导方法研究

    Institute of Scientific and Technical Information of China (English)

    王跃华; 胡胜铃; 代勇; 蒋婷婷; 付伟; 杨军

    2011-01-01

    [ Objective ] Rolyploid bulb of Fritillaria cirrhosa D. Don is introduced by tissue culture technique. [ Methods ] The compact callus cell mass of Fritillaria cirrhosa D. Don which are differentiation cultured beforehand are soaked in colchicine solution, and then cultured in the differential medium of MS + 6-BA 1.0 mg/L + NAA 0.3 mg/L. Polyploid bulb are obtained by induction differentiation culture. [ Result] Colchicine solution of 1.2 g/L is used to douse the compact callus cell mass of Fritillaria cirrhosa D. Don for 24 h. The highest inductivity of polyploid bulb was 83.3%. [ Conclusion] Chromosome number is 2n =4x =48 and the total alkaloids content is detected to be 0. 249%. It indicates that it is feasible to obtain high quality and high yield of Fritillaria cirrhosa D. Don polyploid bulb by tissue culture technique.%[目的]利用组织培养技术快速诱导生成卷叶贝母(Fritillaria cirrhosa D.Don)多倍体鳞茎.[方法]用一定浓度的秋水仙素溶液浸泡经预分化培养的卷叶贝母紧密愈伤组织团块,再经过诱导分化培养获得多倍体鳞茎.[结果]用浓度为1.2 g/L的秋水仙素溶液浸泡处理卷叶贝母紧密愈伤组织团块24 h后,接种在Ms+6-BA 1.0 mg/L+NAA 0.3 mg/L的分化培养基上培养,其多倍体鳞茎的诱导率最高为83.3%,细胞染色体数为2n=4x=48,总生物碱含量为0.249%.[结论]用组织培养技术获得优质高产卷叶贝母多倍体鳞茎的方法是可行的.

  15. Catharanthus roseus (L. G. Don - plant regeneration and alkaloids content

    Directory of Open Access Journals (Sweden)

    Mirosława Furmanowa

    2014-02-01

    Full Text Available We describe here a regeneration of plantlets of Catharanthus roseus (L. G. Don from shoot tips and axillary buds. Shoot tips were excised from 7-day-old seedlings and were incubated in solid Nitsch and Nitsch (NN medium supplemented with kinetin, benzyladenine (BA, indole-3-butyric acid (IBA and β-indolylacetic acid (IAA in various combinations. After two months in culture, regenerated rooted plantlets were cut and transferred to a new medium; the explants contained shoot tips or axillary buds. Four passages were done. We obtained about 200 rooting plantlets from one seedling. Then the plantlets were transferred to the soil and they grew under a foil tent. After five months of vegetation they were collected, dried and weighed. Chemical investigations of leaves of these plants were done. The vindoline and catharanthine were dominant alkaloids in the juvenile stage of plants (before blooming. Total amount of alkaloids, equal 2.95%, was gravimetrically determined in leaves of plants, after 4th passage, regenerated in vitro on NN medium supplemented with kinetin and IBA.

  16. Increasing trends of dissolved organic nitrogen (DON) in temperate forests under recovery from acidification in Flanders, Belgium.

    Science.gov (United States)

    Verstraeten, Arne; Verschelde, Pieter; De Vos, Bruno; Neirynck, Johan; Cools, Nathalie; Roskams, Peter; Hens, Maarten; Louette, Gerald; Sleutel, Steven; De Neve, Stefaan

    2016-05-15

    We evaluated trends (2005-2013) and patterns of dissolved organic nitrogen (DON) and its ratio with dissolved organic carbon (DOC), DOC:DON in atmospheric deposition and soil solution of five Level II plots of the International Co-operative Programme on Assessment and Monitoring of Air Pollution Effects on Forests (ICP Forests) in Flanders, Northern Belgium. The primary aim was to confirm positive postulated trends in DON levels and DOC:DON under on-going recovery from acidification. The DON concentrations (0.95-1.41mgL(-1)) and fluxes (5.6-8.3kgha(-1)y(-1)) in throughfall were about twice as high compared to precipitation in the open field (0.40-0.48mgL(-1), 3.0-3.9kgha(-1)y(-1)). Annual soil profile leaching losses of DON varied between 1.2 and 3.7kgha(-1)y(-1). The highest soil DON concentrations and fluxes were observed beneath the O horizon (1.84-2.36mgL(-1), 10.1-12.3kgha(-1)y(-1)). Soil solution concentrations and fluxes of DON showed significant increasing trends. Temporarily soil solution DOC:DON rose following an exceptionally long spring drought in 2007, suggesting an effect of drying and rewetting on DOM composition. Further research is needed to test the dependence of DON and DOC:DON on factors such as latitude, forest cover, length of the growing season, hydrology and topography. Nonetheless, even with considerable variation in soil type, level of base saturation, and soil texture in the five included ICP Forests Level II plots, all data revealed a proportionally larger positive response of DON flux than DOC to recovery from acidification. PMID:26906698

  17. Nuclear physics (of the cell, not the atom)

    Science.gov (United States)

    Pederson, Thoru; Marko, John F.

    2014-01-01

    The nucleus is physically distinct from the cytoplasm in ways that suggest new ideas and approaches for interrogating the operation of this organelle. Chemical bond formation and breakage underlie the lives of cells, but as this special issue of Molecular Biology of the Cell attests, the nonchemical aspects of cell nuclei present a new frontier to biologists and biophysicists. PMID:25368422

  18. Risk Acceptance Personality Paradigm: How We View What We Don't Know We Don't Know

    Science.gov (United States)

    Massie, Michael J.; Morris, A. Terry

    2011-01-01

    The purpose of integrated hazard analyses, probabilistic risk assessments, failure modes and effects analyses, fault trees and many other similar tools is to give managers of a program some idea of the risks associated with their program. All risk tools establish a set of undesired events and then try to evaluate the risk to the program by assessing the severity of the undesired event and the likelihood of that event occurring. Some tools provide qualitative results, some provide quantitative results and some do both. However, in the end the program manager and his/her team must decide which risks are acceptable and which are not. Even with a wide array of analysis tools available, risk acceptance is often a controversial and difficult decision making process. And yet, today's space exploration programs are moving toward more risk based design approaches. Thus, risk identification and good risk assessment is becoming even more vital to the engineering development process. This paper explores how known and unknown information influences risk-based decisions by looking at how the various parts of our personalities are affected by what they know and what they don't know. This paper then offers some criteria for consideration when making risk-based decisions.

  19. The Authors of the Ingenious Hidalgo Don Quixote of La Mancha

    Directory of Open Access Journals (Sweden)

    Branka Kalenić Ramšak

    2008-12-01

    Full Text Available Even today literary criticism still considers the novel Don Quixote the first modern European novel because it fundamentally changes both the concept of literary creation and the findings regarding literary reception. Miguel de Cervantes Saavedra published the first part of his chivalric novel in 1605 in Madrid with the title El ingenioso hidalgo don Quijote de la Mancha. He published the second part, titled El ingenioso caballero don Quijote de la Mancha, ten years later (i.e., in 1615, again in Madrid. Why did Cervantes change the title in the second part of his novel and thus transform Don Quixote the hidalgo ‘nobleman’ into Don Quixote the caballero ‘knight, nobleman, horseman’? In Spanish literature of Cervantes’ time, writers often borrowed texts from one another, wrote sequels to them, and reworked them into humorous poems, jocular one-act plays, or unusual parodies. The Baroque concept of imitation was not understood as plagiarism, but rather as a positive approach to creativity. One of Cervantes’ most enthusiastic imitators was Alonso Fernández de Avellaneda. He hated Cervantes, but loved Don Quixote so much that he wrote a sequel to it in the form of a chivalric novel. In 1614, Avellaneda published his novel titled Segundo tomo del ingenioso hidalgo don Quijote de la Mancha (The Second Part of the Ingenious Hidalgo of La Mancha in Tarragona. Who is hidden behind the name Alonso Fernández de Avellaneda? To date, literary history has not been able to establish with certainty who the author of this “second part” was; this work represents the greatest literary mystery of all time in Spanish literature. In the 1960s a theory developed among Cervantes experts that for now seems to be the most convincing in determining Avellaneda’s true identity. In his article “ El Quijote y los libros” (Don Quixote and Books of 1969, Martín de Riquer presented the first well-founded hypothesis claiming that the writer Alonso Fern

  20. The don't know option in progress testing.

    Science.gov (United States)

    Ravesloot, C J; Van der Schaaf, M F; Muijtjens, A M M; Haaring, C; Kruitwagen, C L J J; Beek, F J A; Bakker, J; Van Schaik, J P J; Ten Cate, Th J

    2015-12-01

    Formula scoring (FS) is the use of a don't know option (DKO) with subtraction of points for wrong answers. Its effect on construct validity and reliability of progress test scores, is subject of discussion. Choosing a DKO may not only be affected by knowledge level, but also by risk taking tendency, and may thus introduce construct-irrelevant variance into the knowledge measurement. On the other hand, FS may result in more reliable test scores. To evaluate the impact of FS on construct validity and reliability of progress test scores, a progress test for radiology residents was divided into two tests of 100 parallel items (A and B). Each test had a FS and a number-right (NR) version, A-FS, B-FS, A-NR, and B-NR. Participants (337) were randomly divided into two groups. One group took test A-FS followed by B-NR, and the second group test B-FS followed by A-NR. Evidence for impaired construct validity was sought in a hierarchical regression analysis by investigating how much of the participants' FS-score variance was explained by the DKO-score, compared to the contribution of the knowledge level (NR-score), while controlling for Group, Gender, and Training length. Cronbach's alpha was used to estimate NR and FS-score reliability per year group. NR score was found to explain 27 % of the variance of FS [F(1,332) = 219.2, p trade-off between systematic and random measurement error. PMID:25912621

  1. Pharmacognostical studies of leaves of Combretum albidum G. Don

    Directory of Open Access Journals (Sweden)

    Ashish S Zalke

    2013-01-01

    Full Text Available Background: Combretum albidum Don belonging to family Combretaceae is an unexplored medicinal plant in the Indian medicinal system. According to ethnobotanical information, the leaves are used in the treatment of peptic ulcer and its fruits are used in diarrhoea and dysentery. Stem bark is used in the treatment of jaundice and skin diseases. The problem encountered in standardisation of this medicinal plant is its identification by source. Materials and Methods: The pharmacognostical studies were carried out in terms of organoleptic, macroscopic, microscopic, physicochemical, florescence and phytochemical analysis. Physicochemical parameters such as total ash, moisture content and extractive values are determined by World Health Organization guidelines. The microscopic features of leaf components are observed with Nikon lab photo device with microscopic units. Results: Macroscopically, the leaves are simple, obovate in shape, acuminate apex, entire margin and smooth surface. Microscopically, the leaves showed a large vascular strand that consists of thick walled xylem elements mixed with xylem fibres and phloem which is present in a thin layer along inner and outer portions of xylem. External to the xylem occur a thin line of sclerenchyma. Powder microscopy revealed glandular trichomes in the adaxial epidermal peelings also shows the non-glandular trichomes fairly common in powder and epidermis with anisocytic stomata. Vessels elements are narrow, long, cylindrical and dense multi-seriate bordered pits. Xylem fibres are thin and long, with thick walls, which are lignified. Preliminary phytochemical screening showed the presence of carbohydrate, glycoside, saponin, flavonoid, phytosterols and phenolic compounds. Conclusions: The results of the study can serve as a valuable source of pharmacognostic information as suitable standards for identification of this plant material in future investigations and applications.

  2. Deoxynivalenol affects in vitro intestinal epithelial cell barrier integrity through inhibition of protein synthesis

    International Nuclear Information System (INIS)

    Deoxynivalenol (DON), one of the most common mycotoxin contaminants of raw and processed cereal food, adversely affects the gastrointestinal tract. Since DON acts as a protein synthesis inhibitor, the constantly renewing intestinal epithelium could be particularly sensitive to DON. We analyzed the toxicological effects of DON on intestinal epithelial protein synthesis and barrier integrity. Differentiated Caco-2 cells, as a widely used model of the human intestinal barrier, were exposed to realistic intestinal concentrations of DON (50, 500 and 5000 ng/ml) during 24 h. DON caused a concentration-dependent decrease in total protein content associated with a reduction in the incorporation of [3H]-leucine, demonstrating its inhibitory effect on protein synthesis. DON simultaneously increased the paracellular permeability of the monolayer as reflected through a decreased transepithelial electrical resistance associated with an increased paracellular flux of the tracer [3H]-mannitol. A concentration-dependent reduction in the expression level of the tight junction constituent claudin-4 was demonstrated by Western blot, which was not due to diminished transcription, increased degradation, or NF-κB, ERK or JNK activation, and was also observed for a tight junction independent protein, i.e. intestinal alkaline phosphatase. These results demonstrate a dual toxicological effect of DON on differentiated Caco-2 cells consisting in an inhibition of protein synthesis as well as an increase in monolayer permeability, and moreover suggest a possible link between them through diminished synthesis of the tight junction constituent claudin-4.

  3. The discovery of cometary activity in near-Earth asteroid (3552) Don Quixote

    Energy Technology Data Exchange (ETDEWEB)

    Mommert, Michael; Harris, Alan W. [Institute of Planetary Research, German Aerospace Center (DLR), Rutherfordstr. 2, D-12489 Berlin (Germany); Hora, Joseph L.; Smith, Howard A. [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138-1516 (United States); Reach, William T. [Universities Space Research Association, Stratospheric Observatory for Infrared Astronomy, MS 232-11, NASA Ames Research Center, Moffett Field, CA 94035 (United States); Emery, Joshua P. [Department of Earth and Planetary Sciences, University of Tennessee, 1412 Circle Dr., Knoxville, TN 37996 (United States); Thomas, Cristina A. [NASA Postdoctoral Program Fellow, NASA Goddard Space Flight Center, 8800 Greenbelt Rd, Greenbelt, MD 20771 (United States); Mueller, Michael [SRON Netherlands Institute for Space Research, Postbus 800, 9700 AV Groningen (Netherlands); Cruikshank, Dale P. [NASA Ames Research Center, Moffett Field, CA 94035 (United States); Trilling, David E. [Department of Physics and Astronomy, Northern Arizona University, P.O. Box 6010, Flagstaff, AZ 86011 (United States); Delbo, Marco [UNS-CNRS-Observatoire de la Cote d' Azur, BP4229, F-06304 Nice Cedex 4 (France)

    2014-01-20

    The near-Earth object (NEO) population, which mainly consists of fragments from collisions between asteroids in the main asteroid belt, is thought to include contributions from short-period comets as well. One of the most promising NEO candidates for a cometary origin is near-Earth asteroid (3552) Don Quixote, which has never been reported to show activity. Here we present the discovery of cometary activity in Don Quixote based on thermal-infrared observations made with the Spitzer Space Telescope in its 3.6 and 4.5 μm bands. Our observations clearly show the presence of a coma and a tail in the 4.5 μm but not in the 3.6 μm band, which is consistent with molecular band emission from CO{sub 2}. Thermal modeling of the combined photometric data on Don Quixote reveals a diameter of 18.4{sub −0.4}{sup +0.3} km and an albedo of 0.03{sub −0.01}{sup +0.02}, which confirms Don Quixote to be the third-largest known NEO. We derive an upper limit on the dust production rate of 1.9 kg s{sup –1} and derive a CO{sub 2} gas production rate of (1.1 ± 0.1) × 10{sup 26} molecules s{sup –1}. Spitzer Infrared Spectrograph spectroscopic observations indicate the presence of fine-grained silicates, perhaps pyroxene rich, on the surface of Don Quixote. Our discovery suggests that CO{sub 2} can be present in near-Earth space over a long time. The presence of CO{sub 2} might also explain that Don Quixote's cometary nature remained hidden for nearly three decades.

  4. 77 FR 64973 - Don W. Gilbert Hydro Power, LLC; Notice of Application Accepted for Filing With the Commission...

    Science.gov (United States)

    2012-10-24

    ... penstock; (3) a powerhouse containing two 45-kilowatt (kW) turbine/ generator units for a total installed... Energy Regulatory Commission Don W. Gilbert Hydro Power, LLC; Notice of Application Accepted for Filing.... Date filed: May 30, 2012. d. Applicant: Don W. Gilbert Hydro Power, LLC. e. Name of Project:...

  5. Co-Contamination of DON and NIV in Domestic Flour in Japan: Survey, Intake, Reduction and Rapid Assay

    Science.gov (United States)

    Nivalenol (NIV) and Deoxynivalenol (DON) are trichothecene mycotoxins produced by Fusarium spp. that contaminate mainly cereal crops, such as wheat, barley, and maize. These mycotoxins are serious health hazards to humans and domestic animals. In Japan, there have been many reports of DON and NIV ...

  6. PERKEMBANGAN LATISIFER PADA KULTUR KALUS CATHARANTHUS ROSEUS (L G. DON YANG DIINDUKSI DENGAN KOMBINASI ZAT PENGATUR TUMBUH KINETIN + NAA

    Directory of Open Access Journals (Sweden)

    NI NYOMAN DARSINI

    2011-12-01

    Full Text Available The development of laticifer on callus culture of Catharanthus roseus (L G Don in Zenk medium supplemented with combination of plant growth regulator kinetin + NAA was studied. The explants were taken from the second folium from shoot apex. Development of laticifer was observed using descriptive analysis method for callus anatomy and percentage of laticifer was observed during 4–14 weeks of callus development. The percentage of laticifer was determined by counting the average number of the laticifer and the average number of surrounding cells in every optical field of few under light microscope. The results showed that early development of laticifer which was induced with plant regulator growth kinetin + NAA was found in the 9 weeks old callus. The laticifer has specific characteristics i.e. thicker cell wall and longer cell than sorounding cell. Elongated laticifer was observed at 12 weeks old callus. The highest percentage of laticifer on callus C. roseus induced with combination of kinetin and NAA was found in 12 weeks old callus i.e. 0,12%. At 13 and 14 weeks old callus, the anatomy of laticifer was similar to that at 12 weeks old callus, but the percentage was lower.

  7. La reciprocidad y el don no son la misma cosa

    Directory of Open Access Journals (Sweden)

    Ricardo Gabriel Abduca

    2007-12-01

    Full Text Available El autor se propone mostrar por qué la noción de reciprocidad, aunque ha sido muchas veces tomada como sinónimo del fenómeno de intercambios de dones, corresponde a diversas situaciones sociales, por lo cual tiene poca utilidad descriptiva y explicativa. Examina algunos hitos de la historia antropológica, mostrando que más que un concepto, 'reciprocidad' es un palimpsesto sobre el cual, de Durkheim a Polanyi, se han ido escribiendo variadas nociones. En cambio, sugiere una definición de don más estricta, cercana a las ideas de Mauss y acorde con los avances de la etnografía y la teoría contemporánea. Sugiere distinguir entre circuitos cerrados de dones, donde circulan bienes de consumo final, y circuitos abiertos, donde los dones que circulan condensan el conjunto de las relaciones sociales.O objetivo deste texto é mostrar que a expressão 'reciprocidade', ainda foi considerada como sinônimo de 'câmbio de domes', é outra coisa. O autor sugere um breve esquema de alguns marcos do desenvolvimento da noção de 'reciprocidade' em a teoria antropológica, mostrando que o sentido dela expressão foi mudando até assemelhar um palimpsesto com múltiplas rescritas, no processo que vá de Durkheim a Polanyi. Defende um conceito de dom próximo às idéias de Mauss e a certos avances dela etnografia contemporânea e a teoria social. Distingue deus tipos de circuitos de circulação de domes: fechados, onde circulam bens de consumação direita, e apertos, onde circulam certo tipo de bens que condensam o conjunto das relações sociais.The aim of this text is to show how the notion of 'reciprocity', although have been considered a synonymous of gift exchange, veils different social situations. A brief survey of the history of the use of the word shows how 'reciprocity', from Durkheim to Polanyi, seems as a palimpsest of various notions. Although, he essays a defense of a concept of 'gift' related to the Maussian texts, and closer to some

  8. Un curioso coleccionista del siglo XVII: don Manuel de Porras

    Directory of Open Access Journals (Sweden)

    Agulló y Cobo, Mercedes

    2003-09-01

    Full Text Available Art collecting - an avocation almost exclusive to royalty in Spain - has not had representatives among those who did not belong to the high nobility, not even among artists themselves. In many cases, the word "collection" has been applied to what in reality were mere lists of patrimonial properties. For all of the above, it is especially noteworthy to encounter someone who neither belonged to an elevated class nor was a painter, but who managed to unite, at great personal sacrifice, an important art collection. Manuel de Porras owned interesting and a relatively large number of paintings, sculptures and prints, some of them extremely valuable. As a good collector, in order to avoid the dispersion of these works, he donated them to the Shod Trinitarians in Madrid and the Oratory of Saint Philip Neri in Alcalá, but unfortunately, in spite of his good intentions, nothing has survived.

    El coleccionismo —afición casi exclusiva de Reyes en España— no ha tenido representantes entre personas que no pertenecieran a la alta nobleza, ni entre los propios artistas. En muchos casos lo que se ha venido llamando colecciones son sólo relación de bienes patrimoniales. Por ello, resulta más llamativo encontrar alguien que, sin pertenecer a una clase elevada ni ser artista, lograra reunir —aun a costa de grandes sacrificios que le llevaron al extremo de ser enterrado de limosna— una tan importante colección de obras de arte como el personaje objeto de este artículo: Manuel de Porras, quien contaba con un interesante y elevado (para un particular sin títulos número de pinturas y esculturas, y un importantísimo número de dibujos y estampas, algunas valiosísimas y de alto precio. Desgraciadamente, aunque lo donó —como buen coleccionista, para evitar su dispersión— a los Trinitarios calzados de Madrid y al Oratorio de San Felipe Neri de Alcalá, nada de ello ha llegado a nuestros días.

  9. Don Frane Bulić i pokušaj obnove Ninske biskupije

    OpenAIRE

    Strika, Zvjezdan

    2013-01-01

    Zaslužni hrvatski arheolog don Frane Bulić (1846.-1934.) pratio je budno zbivanja u Hrvatskoj poslije I. svjetskog rata i nestanka Habsburške monarhije; njemu nisu ostale neprimijećene potrebe puka i svećenstva onog dijela Zadarske nadbiskupije koji je pripadao Kraljevini SHS, dok je grad Zadar Rapallskim ugovorom (12. studenog 1920. godine) bio priključen Kraljevini Italiji. Osjećajući potrebu uspješnije i djelotvornije pastorizacije, smatrao je don Frane potrebnim utemeljenje jednog novog b...

  10. Picrasma quassiodes (D. Don) Benn. attenuates lipopolysaccharide (LPS)-induced acute lung injury.

    Science.gov (United States)

    Lee, Jae-Won; Park, Ji-Won; Shin, Na-Rae; Park, So-Yeon; Kwon, Ok-Kyoung; Park, Hyun Ah; Lim, Yourim; Ryu, Hyung Won; Yuk, Heung Joo; Kim, Jung Hee; Oh, Sei-Ryang; Ahn, Kyung-Seop

    2016-09-01

    Picrasma quassiodes (D.Don) Benn. (PQ) is a medicinal herb belonging to the family Simaroubaceae and is used as a traditional herbal remedy for various diseases. In this study, we evaluated the effects of PQ on airway inflammation using a mouse model of lipopolysaccharide (LPS)-induced acute lung injury (ALI) and LPS-stimulated raw 264.7 cells. ALI was induced in C57BL/6 mice by the intranasal administration of LPS, and PQ was administered orally 3 days prior to exposure to LPS. Treatment with PQ significantly attenuated the infiltration of inflammatory cells in the bronchoalveolar lavage fluid (BALF). PQ also decreased the production of reactive oxygen species (ROS) and pro-inflammatory cytokines, such as tumor necrosis factor (TNF)-α and interleukin (IL)-6 in BALF. In addition, PQ inhibited airway inflammation by reducing the expression of inducible nitric oxide synthase (iNOS) and by increasing the expression of heme oxygenase-1 (HO-1) in the lungs. Furthermore, we demonstrated that PQ blocked the activation of mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) in the lungs of mice with LPS-induced ALI. In the LPS-stimulated RAW 264.7 cells, PQ inhibited the release of pro-inflammatory cytokines and increased the mRNA expression of monocyte chemoattractant protein-1 (MCP-1). Treatment with PQ decreased the translocation of nuclear factor (NF)-κB to the nucleus, and increased the nuclear translocation of nuclear factor erythroid-2-related factor 2 (Nrf2) and the expression of HO-1. PQ also inhibited the activation of p38 in the LPS-stimulated RAW 264.7 cells. Taken together, our findings demonstrate that PQ exerts anti-inflammatory effects against LPS-induced ALI, and that these effects are associated with the modulation of iNOS, HO-1, NF-κB and MAPK signaling. Therefore, we suggest that PQ has therapeutic potential for use in the treatment of ALI. PMID:27431288

  11. Genomic toolboxes for conservation biologists

    OpenAIRE

    Angeloni, F.; Wagemaker, N.; Vergeer, P.; Ouborg, N.J.

    2011-01-01

    Conservation genetics is expanding its research horizon with a genomic approach, by incorporating the modern techniques of next-generation sequencing (NGS). Application of NGS overcomes many limitations of conservation genetics. First, NGS allows for genome-wide screening of markers, which may lead to a more representative estimation of genetic variation within and between populations. Second, NGS allows for distinction between neutral and non-neutral markers. By screening populations on thou...

  12. NMR for chemists and biologists

    CERN Document Server

    Carbajo, Rodrigo J

    2013-01-01

    This book intends to be an easy and concise introduction to the field of nuclear magnetic resonance or NMR, which has revolutionized life sciences in the last twenty years. A significant part of the progress observed in scientific areas like Chemistry, Biology or Medicine can be ascribed to the development experienced by NMR in recent times. Many of the books currently available on NMR deal with the theoretical basis and some of its main applications, but they generally demand a strong background in Physics and Mathematics for a full understanding. This book is aimed to a wide scientific audie

  13. Reproducible Bioinformatics Research for Biologists

    Science.gov (United States)

    This book chapter describes the current Big Data problem in Bioinformatics and the resulting issues with performing reproducible computational research. The core of the chapter provides guidelines and summaries of current tools/techniques that a noncomputational researcher would need to learn to pe...

  14. The Postmodern Don Juan Scoring on the Internet. Representation of Gender and Culture of Late Capitalism in the Film Don Jon (2013)

    OpenAIRE

    Sóley Arngrímsdóttir 1990

    2015-01-01

    This essay explores how the film Don Jon (2013) is an astute criticism on modern society, showing how gender inequality is embedded in media narratives and ultimately institutionalized in pornography. Reading the film through literary theories of the postmodern, such as Frederic Jameson, Jean Baudrillard, Laura Mulvey and Judith Butler, the essay demonstrates how the setting and gender relations represented in the film exemplify the culture of late capitalism, with its fragmented sense of his...

  15. Deoxynivalenol induced mouse skin cell proliferation and inflammation via MAPK pathway

    Energy Technology Data Exchange (ETDEWEB)

    Mishra, Sakshi [Food Drug and Chemical Toxicology, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), P.O. Box No. 80, Mahatma Gandhi Marg, Lucknow 226 001 (India); Department of Biochemistry, Banaras Hindu University (BHU), Varanasi (India); Tripathi, Anurag [Food Drug and Chemical Toxicology, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), P.O. Box No. 80, Mahatma Gandhi Marg, Lucknow 226 001 (India); Chaudhari, Bhushan P. [Pathology Laboratory, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), Mahatma Gandhi Marg, PO Box 80, Lucknow 226001, Uttar Pradesh (India); Dwivedi, Premendra D. [Food Drug and Chemical Toxicology, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), P.O. Box No. 80, Mahatma Gandhi Marg, Lucknow 226 001 (India); Pandey, Haushila P. [Department of Biochemistry, Banaras Hindu University (BHU), Varanasi (India); Das, Mukul, E-mail: mditrc@rediffmail.com [Food Drug and Chemical Toxicology, CSIR-Indian Institute of Toxicology Research (CSIR-IITR), P.O. Box No. 80, Mahatma Gandhi Marg, Lucknow 226 001 (India)

    2014-09-01

    Several toxicological manifestations of deoxynivalenol (DON), a mycotoxin, are well documented; however, dermal toxicity is not yet explored. The effect of topical application of DON to mice was studied using markers of skin proliferation, inflammation and tumor promotion. Single topical application of DON (84–672 nmol/mouse) significantly enhanced dermal hyperplasia and skin edema. DON (336 and 672 nmol) caused significant enhancement in [{sup 3}H]-thymidine uptake in DNA along with increased myeloperoxidase and ornithine decarboxylase activities, suggesting tissue inflammation and cell proliferation. Furthermore, DON (168 nmol) caused enhanced expression of RAS, and phosphorylation of PI3K/Akt, ERK, JNK and p38 MAPKs. DON exposure also showed activation of transcription factors, c-fos, c-jun and NF-κB along with phosphorylation of IkBα. Enhanced phosphorylation of NF-κB by DON caused over expression of target proteins, COX-2, cyclin D1 and iNOS in skin. Though a single topical application of DMBA followed by twice weekly application of DON (84 and 168 nmol) showed no tumorigenesis after 24 weeks, however, histopathological studies suggested hyperplasia of the epidermis and hypertrophy of hair follicles. Interestingly, intestine was also found to be affected as enlarged Peyer's patches were observed, suggesting inflammatory effects which were supported by elevation of inflammatory cytokines after 24 weeks of topical application of DON. These results suggest that DON induced cell proliferation in mouse skin is through the activation of MAPK signaling pathway involving transcription factors NFκB and AP-1, further leading to transcriptional activation of downstream target proteins c-fos, c-jun, cyclin D1, iNOS and COX-2 which might be responsible for its inflammatory potential. - Highlights: • Topical application of DON enhanced epidermal inflammation and cell proliferation. • DON follows PI3K/Akt/MAPK signaling cascade, with activation of AP-1 and NF

  16. Deoxynivalenol induced mouse skin cell proliferation and inflammation via MAPK pathway

    International Nuclear Information System (INIS)

    Several toxicological manifestations of deoxynivalenol (DON), a mycotoxin, are well documented; however, dermal toxicity is not yet explored. The effect of topical application of DON to mice was studied using markers of skin proliferation, inflammation and tumor promotion. Single topical application of DON (84–672 nmol/mouse) significantly enhanced dermal hyperplasia and skin edema. DON (336 and 672 nmol) caused significant enhancement in [3H]-thymidine uptake in DNA along with increased myeloperoxidase and ornithine decarboxylase activities, suggesting tissue inflammation and cell proliferation. Furthermore, DON (168 nmol) caused enhanced expression of RAS, and phosphorylation of PI3K/Akt, ERK, JNK and p38 MAPKs. DON exposure also showed activation of transcription factors, c-fos, c-jun and NF-κB along with phosphorylation of IkBα. Enhanced phosphorylation of NF-κB by DON caused over expression of target proteins, COX-2, cyclin D1 and iNOS in skin. Though a single topical application of DMBA followed by twice weekly application of DON (84 and 168 nmol) showed no tumorigenesis after 24 weeks, however, histopathological studies suggested hyperplasia of the epidermis and hypertrophy of hair follicles. Interestingly, intestine was also found to be affected as enlarged Peyer's patches were observed, suggesting inflammatory effects which were supported by elevation of inflammatory cytokines after 24 weeks of topical application of DON. These results suggest that DON induced cell proliferation in mouse skin is through the activation of MAPK signaling pathway involving transcription factors NFκB and AP-1, further leading to transcriptional activation of downstream target proteins c-fos, c-jun, cyclin D1, iNOS and COX-2 which might be responsible for its inflammatory potential. - Highlights: • Topical application of DON enhanced epidermal inflammation and cell proliferation. • DON follows PI3K/Akt/MAPK signaling cascade, with activation of AP-1 and NF-κB.

  17. WImpiBLAST: web interface for mpiBLAST to help biologists perform large-scale annotation using high performance computing.

    Directory of Open Access Journals (Sweden)

    Parichit Sharma

    Full Text Available The function of a newly sequenced gene can be discovered by determining its sequence homology with known proteins. BLAST is the most extensively used sequence analysis program for sequence similarity search in large databases of sequences. With the advent of next generation sequencing technologies it has now become possible to study genes and their expression at a genome-wide scale through RNA-seq and metagenome sequencing experiments. Functional annotation of all the genes is done by sequence similarity search against multiple protein databases. This annotation task is computationally very intensive and can take days to obtain complete results. The program mpiBLAST, an open-source parallelization of BLAST that achieves superlinear speedup, can be used to accelerate large-scale annotation by using supercomputers and high performance computing (HPC clusters. Although many parallel bioinformatics applications using the Message Passing Interface (MPI are available in the public domain, researchers are reluctant to use them due to lack of expertise in the Linux command line and relevant programming experience. With these limitations, it becomes difficult for biologists to use mpiBLAST for accelerating annotation. No web interface is available in the open-source domain for mpiBLAST. We have developed WImpiBLAST, a user-friendly open-source web interface for parallel BLAST searches. It is implemented in Struts 1.3 using a Java backbone and runs atop the open-source Apache Tomcat Server. WImpiBLAST supports script creation and job submission features and also provides a robust job management interface for system administrators. It combines script creation and modification features with job monitoring and management through the Torque resource manager on a Linux-based HPC cluster. Use case information highlights the acceleration of annotation analysis achieved by using WImpiBLAST. Here, we describe the WImpiBLAST web interface features and architecture

  18. WImpiBLAST: web interface for mpiBLAST to help biologists perform large-scale annotation using high performance computing.

    Science.gov (United States)

    Sharma, Parichit; Mantri, Shrikant S

    2014-01-01

    The function of a newly sequenced gene can be discovered by determining its sequence homology with known proteins. BLAST is the most extensively used sequence analysis program for sequence similarity search in large databases of sequences. With the advent of next generation sequencing technologies it has now become possible to study genes and their expression at a genome-wide scale through RNA-seq and metagenome sequencing experiments. Functional annotation of all the genes is done by sequence similarity search against multiple protein databases. This annotation task is computationally very intensive and can take days to obtain complete results. The program mpiBLAST, an open-source parallelization of BLAST that achieves superlinear speedup, can be used to accelerate large-scale annotation by using supercomputers and high performance computing (HPC) clusters. Although many parallel bioinformatics applications using the Message Passing Interface (MPI) are available in the public domain, researchers are reluctant to use them due to lack of expertise in the Linux command line and relevant programming experience. With these limitations, it becomes difficult for biologists to use mpiBLAST for accelerating annotation. No web interface is available in the open-source domain for mpiBLAST. We have developed WImpiBLAST, a user-friendly open-source web interface for parallel BLAST searches. It is implemented in Struts 1.3 using a Java backbone and runs atop the open-source Apache Tomcat Server. WImpiBLAST supports script creation and job submission features and also provides a robust job management interface for system administrators. It combines script creation and modification features with job monitoring and management through the Torque resource manager on a Linux-based HPC cluster. Use case information highlights the acceleration of annotation analysis achieved by using WImpiBLAST. Here, we describe the WImpiBLAST web interface features and architecture, explain design

  19. Microsporogênese em Coffea stenophylla G. Don e C. salvatrix Swynn et Phil Microsporogenesis in Coffea stenophylla G. Don and C. salvatrix Swynn et. Phil

    Directory of Open Access Journals (Sweden)

    Dixier M. Medina

    1969-01-01

    Full Text Available Estudou-se a microsporogênese de Coffea stenophylla,G. Don e de C. salvatrixSwynn et Phil, espécies diplóides de Coffea, introduzidas respectivamente da Guatemala e da Colômbia e mantidas em coleção no Instituto Agronômico de Campinas. O aspecto dos cromossomos é semelhante ao já descrito para outras espécies do gênero. O emparelhamento ocorre em todos os cromossomos, desde os estádios iniciais da prófase até a primeira metáfase; os 11 bivalentes formados separam-se normalmente nas anáfases, e os micróspores resultantes são haplóides, com n= 11 cromossomos. O comportamento cromossômico nas diversas fases da meiose é regular na maioria das células mães. O número de bivalentes com três, dois e um quiasma é, em média, 1, 4 e 6 para C. stenophyllae 1, 3 e 7 para C. salvatrix, num total de 16 e 17 quiasmas por célula, respectivamente. Parece haver em C. stenophyllavariação maior nos tipos de distribuição dos quiasmas pelos bivalentes. As irregularidades encontradas referem-se principalmente à ocorrência de monovalentes, de retardatários nas anáfases e à distribuição desigual dos cromossomos para os pólos. Discute-se a possibilidade de formação de gametas com números de cromossomos diferentes de 11 e de gametas com conjuntos não balanceados, além dos haplóides normais.Studies on the microsporogenesis in Coffea stenophylla and C. salvatrix demonstrated that the meiotic chromosomes of both species resemble those of the other diploid species of the genus Coffea. Complete chromosome pairing was observed from early prophase till first metaphase, "with 11 bivalents being formed in most of the cells. These bivalents fall apart in a quite regular manner at the two anaphases. The average numbers of bivalents with three, two and one chiasma at first metaphase are 1, 4 and 6 por C. stenophylla and 1, 3 and 7 for C. salvatrix, the mean number of chiasmata per cell being 17 and 16 respectively. Despite of the very

  20. Studies on cytological, physiological and genetic characteristics in somatic mutant strains of Sugi (Cryptomeria japonica D. Don)

    International Nuclear Information System (INIS)

    From microscopic observation of the pollen of induced mutant strains in Sugi (Cryptomeria japonica D. Don), it was found that there were large differences in pollen fertility among the mutant strains, and that it deviated year to year from the mother plants. The large differences in frequency of sterile pollen among mutant strains depended on the genetic characteristics of each mutant strain. Higher frequencies of sterile pollen were observed at the terminal part of branchlets in some mutant strains, and this was considered to be induced by the lateness of flower-bud formation at low temperature conditions in late summer. Delayed formation and gibberellic acid treatment applied for flower induction resulted in low fertility and abnormality of pollen in mutant strains. Chromosome aberration in mutant strains was caused either by gamma irradiation or by some mutational events that responded to environmental conditions. In the former case, aberration might have been maintained for a long period through vegetative propagation. Some of the irregularities were due to mitotic cell division, because cells with micronuclei at the pacytene stage in pollen mother cells and with fragments at MI were observed. Somatic mutability of Kuma-sugi mutants after re-irradiation was investigated. From waxless mutants morphological somatic mutations, which have fat or stout stems and thick and short needles, were frequently produced, whereas from morphological mutants the lowest somatic mutation frequency was induced. In some mutant strains higher rooting ability than the mother plants was found, and the possibility of character improvement was pointed out. (author)

  1. Encouraging and Clarifying "Don't Know" Responses Enhances Interview Quality

    Science.gov (United States)

    Scoboria, Alan; Fisico, Stephanie

    2013-01-01

    Investigative interviewers seek to obtain complete and accurate accounts of events from witnesses. Two studies examined the influence of instructions about the use of don't know (DK) responses and of clarifying the meanings of DK responses on the quality of responding to questioning. Participants watched a video, and after a delay (Study 1, 30…

  2. Magnetic observations of pulsating B and Be stars with ESPaDOnS and Narval

    NARCIS (Netherlands)

    J. Silvester; C. Neiner; H.F. Henrichs; G.A. Wade; E. Alecian; V. Petit

    2008-01-01

    Discoveries of magnetic fields in pulsating B and Be stars have been claimed from low-resolution spectropolarimetric observations with FORS1 at VLT. We used the new generation of high-resolution spectropolarimeters, ESPaDOnS at CFHT and NARVAL at TBL, to check for the existence of these fields. We f

  3. A new phenylethanoid glycoside from Rabdosia lophanthoides (Buch.-Ham.ex D.Don)Hara.

    Institute of Scientific and Technical Information of China (English)

    Wei Sheng Feng; Xin Yu Zang; Xiao Ke Zheng; Yan Zhi Wang

    2009-01-01

    A new phenylethanoid glycoside,3-hydroxy-4-methoxy-β-phenylethoxy-P-α-L-rhamnopyranosyl-(1-3)-2-O-acetyl-O-β-D-glucopyranoside,named Iophanthoside A,was isolated from Rabdosia lophanthoides(Buch.-Ham.ex D.Don)Hara.Its structurewas determined by spectroscopic evidences.

  4. The Don Bosco Way to Train Disadvantaged Youth. Discussion Paper No. 92.

    Science.gov (United States)

    Mashek, R. W.

    Founded by Don Bosco in 1859 in Turin, Italy, the Salesian Society helps disadvantaged youth in over 100 countries, according to 1991 data. Salesians operate technical training institutes and run numerous informal shops and training courses within their schools, orphanages, street children shelters, and youth centers. The institutes are financed…

  5. "They Don't Know Anything!": Latinx Immigrant Students Appropriating the Oppressor's Voice

    Science.gov (United States)

    Monzó, Lilia D.

    2016-01-01

    This article discusses internalized oppression among Latinx communities through a revolutionary critical pedagogy. Data from a two-year ethnography of Latinx immigrant families show that students were developing deficit perspectives toward their parents, claiming that "they don't know anything," based on their positioning as powerless…

  6. Video-Astronaut Don Pettit Discusses the Value of Space Research

    Science.gov (United States)

    2003-01-01

    Saturday Morning Science, the science of opportunity series of applied experiments and demonstrations, performed aboard the International Space Station (ISS) by Expedition 6 astronaut Dr. Don Pettit, revealed some remarkable findings. In this video clip, Dr. Pettit speculates on the value of space research and the opportunities it offers for understanding nature's mysteries.

  7. Rap Music in School Counseling Based on Don Elligan's Rap Therapy

    Science.gov (United States)

    Gonzalez, Tiphanie; Hayes, B. Grant

    2009-01-01

    In 2000, Don Elligan introduced Rap Therapy as a psychotherapeutic intervention for working with at-risk youths, primarily African American males whose identities were highly influenced by rap music. Rap music can engage a population of youth who often enter counseling apprehensively (Elligan 2000, 2004; Tillie-Allen, 2005). This article reviews…

  8. "Boys Don't Cry," a Movie Review: Implications for Counseling Persons Who Are Transgendered

    Science.gov (United States)

    Aguilar, Jinnelle Veronique

    2010-01-01

    People who fall under the umbrella of transgender belong to one of the most marginalized groups within society. Many who do not pass within the gender binary categories imposed by social norms experience discrimination and marginalization. In this article, the author discusses the film "Boys Don't Cry" and introduces the reader to the varieties of…

  9. Don't I Wish My Professor Was Hot like Me

    Science.gov (United States)

    Klink, William

    2010-01-01

    In this article, the author presents his reflection about the song "Don't Cha" by the Pussy Cat Dolls (2006), which makes a strong statement in a postmodern way about women, and sometimes men, who see themselves in the world as central players in a dramatic narrative that highlights their own victimization as happy, powerful, and glorious. These…

  10. Why I don't kill myself : [poems] / Paul-Eerik Rummo

    Index Scriptorium Estoniae

    Rummo, Paul-Eerik, 1942-

    2003-01-01

    Autori lühitutvustus lk. 262. Sisu: Why I don't kill myself ; The sky stoops over the earth ; Clinging ; Crooning. Orig.: Miks ma end ära ei tapa ; "Taevas on kummargil üle maa..." ; Kinni hoidmas ; Poolüminal

  11. Therapists' continuations following I don't know-responses of adolescents in psychotherapy

    NARCIS (Netherlands)

    Jager, Margot; Huiskes, Mike; Metselaar, Janneke; Knorth, Erik J; De Winter, Andrea F; Reijneveld, Sijmen A

    2016-01-01

    OBJECTIVE: In psychotherapy clients' I don't know-responses (IDK-responses) to therapists' questions are typically considered to be non-cooperating behaviors. How therapists actually handle these behaviors remains unclear. This study therefore aims to assess client-therapist interactions following I

  12. Don Juan is written for Prague – More need not be said”

    Czech Academy of Sciences Publication Activity Database

    Jonášová, Milada

    Praha : Národní divadlo, 2012 - (Blachut, B.), s. 49-66 ISBN 978-80-7258-383-6 R&D Projects: GA ČR GAP409/12/2563 Institutional support: RVO:68378076 Keywords : Mozart: Don Giovanni * Prague * opera Subject RIV: AL - Art, Architecture, Cultural Heritage

  13. Free Radical Scavenging Activity and HPLC Analysis of Araucaria cunninghamii Aiton ex D. Don Leaf Extract

    Directory of Open Access Journals (Sweden)

    Vandana Gautam

    2014-08-01

    Full Text Available Background: Several diseases are caused in the body due to oxidative stress of free radicals. The objectives of the present study were to investigate the antioxidant activity of Araucaria cunninghamii Aiton ex D. Don (Araucariaceae leaf extract. The dried leaves of Araucaria cunninghamii Aiton ex D. Don (Araucariaceae were extracted with 80% methanol. The antioxidant activity of the extract was predicted through in vitro model systems such as 1,1-diphenyl-2-picryl hydrazyl (DPPH, reducing power and nitric oxide scavenging assays. The total phenolic, flavonoid and tannin content of the extract were also estimated in order to draw the correlation with their bioactivities. The extract was also investigated for several polyphenolic compounds using High-performance liquid chromatography (HPLC. Results: The antioxidant activity of extract was found to be excellent in all the three assays. A positive correlation between the antioxidant activity and phenolic content was observed. HPLC analysis identified the presence of Gallic acid, Catechin, Chlorogenic acid, Epicatechin, Caffeic acid, Umbelliferone, Ellagic acid, Quercetin and Kaempferol in the methanol leaf extract of Araucaria cunninghamii Aiton ex D. Don. Conclusions: The results of the present study point towards the fact that Araucaria cunninghamii Aiton ex D. Don leaves possess good antioxidant potential. The strong antioxidant activity can be correlated with the polyphenolic compounds present in the leaves.

  14. Have a Baby or Young Child With a Cold? Most Don't Need Medicines

    Science.gov (United States)

    ... For Consumers Home For Consumers Consumer Updates Have a Baby or Young Child With a Cold? Most Don't Need Medicines Share Tweet ... the throat. back to top When to Call a Doctor Not every sniffle or cough merits a ...

  15. Etude des variations de la teneur en isoflavones et de leur composition dans le germe et le cotylédon de la graine de soja [Glycine max (L.) Merrill

    OpenAIRE

    Rasolohery, Claudine Aimée

    2007-01-01

    La graine de soja est très riche en isoflavones, molécules réparties entre les cotylédons et le germe. Ce travail porte sur l'étude de la variabilité et de la cinétique d'accumulation des isoflavones dans ces deux fractions de la graine. Les isoflavones du germe (daidzéine et glycitéine) ont une concentration 5 à 10 fois supérieure à celle des cotylédons (composés de génistéine et daidzéine). Alors que les pourcentages des différentes isoflavones dans les deux compartiments sont principalemen...

  16. 让英语课堂沐浴德育的春风——Unit12 Don't eat in class教学设计

    Institute of Scientific and Technical Information of China (English)

    崔丽娟

    2010-01-01

    @@ 一、教学目标 1.知识目标 本节课学生掌握下列新单词和句型: rule,hallway,fight,classroom,Ms,outside,dining hall,have to,School RuleDon't arrive late for class.Don't fight.Don't run in the hallway.Don't eat in the classroom.Don't listen to music in the classroom.What are the rules?We can't arrive late for class.

  17. The greatest biologist in 20th century—Francis Crick%20世纪最伟大的生物学家——弗朗西斯?克里克

    Institute of Scientific and Technical Information of China (English)

    顾凡及

    2016-01-01

    A biography of UK scientist Francis Crick who is called as the greatest biologist in 20th century is presented in this article. His contributions to life sciences and his approaches to scholarship are also given.%介绍了被称为"20世纪最伟大的生物学家"的英国科学家克里克的生平、对生命科学的贡献和治学之道.

  18. 20世纪最伟大的生物学家——弗朗西斯?克里克%The greatest biologist in 20th century—Francis Crick

    Institute of Scientific and Technical Information of China (English)

    顾凡及

    2016-01-01

    介绍了被称为"20世纪最伟大的生物学家"的英国科学家克里克的生平、对生命科学的贡献和治学之道.%A biography of UK scientist Francis Crick who is called as the greatest biologist in 20th century is presented in this article. His contributions to life sciences and his approaches to scholarship are also given.

  19. Role of cancer stem cells in hepatocarcinogenesis

    OpenAIRE

    Wang, Bo; Jacob, Samson T.

    2011-01-01

    There has been considerable interest in cancer stem cells (CSCs) among cancer biologists and clinicians, most likely because of their role in the heterogeneity of cancer and their potential application in cancer therapeutics. Recent studies suggest that CSCs play a key role in liver carcinogenesis. A small subpopulation of cancer cells with CSC properties has been identified and characterized from hepatocellular carcinoma (HCC) cell lines, animal models and human primary HCCs. Considering the...

  20. Terrorism, Media, and the Ethics of Fiction: Transatlantic Perspectives on Don DeLillo.Eds.Peter Schneck and Philipp Schweighauser.

    OpenAIRE

    Angeliki Tseti

    2011-01-01

    Far from being yet another collection of critical essays in a long array of references on Don DeLillo, Terrorism, Media and the Ethics of Fiction is a valuable addition to the already extensive bibliography on one of the most prolific writers of our times. What differentiates this book from previous collections is its premise, already indicated in the subtitle: Transatlantic Perspectives on Don DeLillo. Indeed, as editors Schneck and Schweighauser state in their introduction “Don DeLillo’s wo...

  1. Terrorism, Media, and the Ethics of Fiction: Transatlantic Perspectives on Don DeLillo.Eds.Peter Schneck and Philipp Schweighauser.

    Directory of Open Access Journals (Sweden)

    Angeliki Tseti

    2011-10-01

    Full Text Available Far from being yet another collection of critical essays in a long array of references on Don DeLillo, Terrorism, Media and the Ethics of Fiction is a valuable addition to the already extensive bibliography on one of the most prolific writers of our times. What differentiates this book from previous collections is its premise, already indicated in the subtitle: Transatlantic Perspectives on Don DeLillo. Indeed, as editors Schneck and Schweighauser state in their introduction “Don DeLillo’s wo...

  2. Penetration of fosfomycin into IPEC-J2 cells in the presence or absence of deoxynivalenol.

    Directory of Open Access Journals (Sweden)

    Guadalupe Martínez

    Full Text Available Fosfomycin (FOS is an antibiotic used in pig farms for treatment and prevention of infections caused by resistant bacteria during the post-weaning period. Antibiotics and non-toxic concentrations of mycotoxins, such as deoxynivalenol (DON are frequently found in the diet of animals. These compounds can establish interactions in the intestinal tract, which could affect and/or modify the penetration of FOS to enterocytes. The aim of this study was to determine the penetration of FOS into IPECJ-2 cells, a cell line derived from the small intestine of piglets, in the presence and absence of DON. The results from this study showed that there was statistically significant difference in the intracellular concentration of FOS between cells incubated with 580 µg/ml FOS and cells incubated with 580 µg/ml FOS and 1 µg/ml DON. The Cmax of the intracellular antibiotic in the culture plates incubated with FOS in absence of DON was 45.81 µg/ml with a tmax of 4 h. When IPEC-2 cells were incubated with FOS and DON the Cmax was 20.06 µg/ml and the tmax was 30 min. It is concluded that the non-toxic concentration of DON on IPEC-J2 cells after short-term exposure, interferes with the pharmacokinetics of the antibiotic.

  3. Design of a Self Don/Doffing Rear Entry Planetary Suit to Interface with a Suit Port/Lock Project

    Data.gov (United States)

    National Aeronautics and Space Administration — Under Phase 1 of subject SBIR, Air-Lock, Incorporated will design a self donning and doffing Rear Entry Hard Upper Torso (REHUT) that interfaces with a suit port....

  4. Upland habitat weed management plan for the Don Edwards San Francisco Bay National Wildlife Refuge Environmental Education Center

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The purpose of this plan is to outline the current status of invasive weeds at the Don Edwards San Francisco Bay NWR Environmental Education Center and to provide a...

  5. A Nutritional Approach for the Management of Deoxynivalenol (DON Toxicity in the Gastrointestinal Tract of Growing Chickens

    Directory of Open Access Journals (Sweden)

    Jürgen Zentek

    2008-12-01

    Full Text Available It has been shown that DON has negative effects on the active transport of some nutrients in the small intestine of chickens. The plausible interactions between food contaminants and natural components could be high. The present study investigated the effects of DON on the presence or absence of dietary inulin on the electrophysiological response of the gut to glucose. Ussing chamber studies were conducted with isolated jejunal epithelia at the age of 35 days. Electrophysiology of the epithelia was recorded and the changes of the short-circuit current (Isc were determined. The addition of Dglucose on the luminal side of the isolated mucosa increased (P 0.05. However, in the dietary inulin supplemented group in both jejunum and colon, the addition of glucose after preincubation of tissues with DON increased the Isc, suggesting that the dietary inulin supplementation of the broilers regulated and improved the glucose absorption in the presence of DON and kept it at normal levels.

  6. Southern San Francisco Bay Colonial Nesting Bird Census 1995-1996: Don Edwards San Francisco Bay National Wildlife Refuge Lands

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This report summarizes the 1995-1996 field season of the San Francisco Bay Bird Observatory (SFBBO) Colonial Waterbird Monitoring Study on the Don Edwards San...

  7. Bair Island Restoration and Management Plan : Don Edwards San Francisco Bay National Wildlife Refuge : Bair Island Ecological Reserve

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — The U.S. Fish and Wildlife Service, Don Edwards San Francisco Bay National Wildlife Refuge, and the California Department of Fish and Game are proposing adoption of...

  8. PENGGI'NAAN MEDIA GAMBAR DAII\\M PEMBELAJARAN MEMBACA KELAS I SD TRAIER DON BOSCO TARAKAN

    OpenAIRE

    Oberta Olvi Wastuti

    2011-01-01

    OBERTA OLVI WASTUTI 2010. PenggunaanMedia Gambar Dalam pembelajaran Membsca Kelot 1 SD Frater Don Bosco Toraltan. Kelurahan Pamusian, Keeernatan Tarakan tengan, Jl Patimura No 1 RT VIII, Pembimbing I H. Sofa, M.Pd, Pembimbing II Drs, Petrus Tena Bean. Tuj"an ,"n"r'* untuk mengetahui perbedaan hasil belajar membaca denean Menggunakan Media Gambar dan Tidak Menggunakan Media Gambar Siswa Kelas I SD Frater Don Bosco Tarakan. Pengambilan sampel, penulis mengambil semua populasi sebanyak ...

  9. MENINGKATKAN HASIL BELAJAR PKN MELALUI MEDIA BERBASIS TIK PADA SISWA KELAS V SD FRATER DON BOSCO TARAKAN

    OpenAIRE

    Ursula Tuto Kemaun

    2011-01-01

    "Tuto Kemaun Ursula 2011, PKN Improve Learning Outcomes hrough ICT-Based Media in class V student of Don Bosco Brother SD Tarakan Year 2010/2011 lessons. Classroom Action Research goal is to improve student learning outcomes in particular grade 5B SD Frater Don Bosco Tarakan through the medium of ICT-based learning civic education so that learning becomes more enjoyable, challenging, and lead to creativity. Design research is done through two cycles, starting from planning, implementation mea...

  10. Real men don't beat their wives!

    OpenAIRE

    Kaur, Dr Gurmeen

    2010-01-01

    Despite the numerous Domestic violence laws, women protection acts and women cells in India at present; physical abuse and domestic violence is still rampant and grossly underreported. This article describes my experience in dealing with victims of domestic abuse as a medical student.

  11. ["Is it an animal inside? "Melanie Klein's unpublished Don Juan Paper (1939)].

    Science.gov (United States)

    Frank, Claudia

    2008-01-01

    Klein had been asked to contribute an article to the birthday number of the International Journal for Jones. The author outlines how she hurriedly wrote a text about Don Juan which, however, was rejected by the editor. Essential parts of it are presented in German translation. The manuscript is discussed in the context of Klein's published work as well as of the relevant contemporary literature. In Klein's view, Don Juan's genitality is determined by oral impulses and fears. By his manic acting out he attempts to ward off a depressive break-down. The paper ends with some reflections about why Klein--ontrary to her intention--failed to revise her manuscript for later publication. PMID:19230330

  12. Migration of Near Earth Asteroid to Jovian-Crosser Asteroid:Case Study 3552 Don Quixote

    CERN Document Server

    Siregar, Suryadi

    2010-01-01

    It is generally recognized that main-belt asteroids (MBAs) and nuclei of extinct comets are the two main sources for the Near-Earth-Asteroids (NEAs). Theoretical studies of NEAs dynamics and numerical modelling of their orbital motions showed that the resonance mechanism for supplying NEAs is quite sufficient to sustain this population. Asteroid 1983 SA, also known as 3552 Don Quixote, is one of Near Earth Asteroids (NEAs) and the most probable candidates for NEAs of the cometary origin. In this work, an investigation on the evolution of the orbit is done by using the SWIFT subroutine package, where the gravitational perturbations of eight planets: Mercury, Venus, Earth, Mars, Jupiter, Saturn, Uranus, and Neptune are considered. Migration of asteroid 3552 Don Quixote from Near Earth Asteroids (NEAs) to Jovian-crosser asteroid is found.

  13. El palacio de don Antonio de Mendoza en Guadalajara. Aspectos decorativos y formales

    Directory of Open Access Journals (Sweden)

    Rodríguez Rebollo, Ángel

    2002-09-01

    Full Text Available The Palace of Don Antonio de Mendoza in Guadalajara was constructed at the beginning of the sixteenth century. In spite of the absence of documentation, there are clear stylistic similarities between this palace and the Calahorra Palace (Granada and the tomb of Cardinal Mendoza. The Codex Escurialensis was used for the decoration of the first floor column capitals as well as the façade decoration.

    El Palacio de Don Antonio de Mendoza en Guadalajara fue construido a principios del XVI. Pese a no estar documentado, las similitudes estilísticas con el Palacio de la Calahorra (Granada y el sepulcro del Cardenal Mendoza son claros. El Codex Excurialensis fue usado en la decoración de los capiteles del primer piso y en la decoración de la fachada principal.

  14. Cultos de oración y "don de lenguas" en grupos pentecostales en Maracaibo

    Directory of Open Access Journals (Sweden)

    Julio García Delgado

    2010-01-01

    Full Text Available En este artículo se analiza el ritual de culto de oración y don de lenguas como movimiento de revitalización y búsqueda de lo sagrado dentro de los grupos pentecostales en Maracaibo, asumiéndose como ritual social y el grupo en un estado de liminaridad colectiva conformando una communitas. Se realizó una entrevista abierta a quien afirma que ha recibido el don de lengua. Los resultados revelan que los cultos de oración son un ritual fundamental en la construcción y consolidación de la communitas proveniente de la limimaridad colectiva, originado por los fuertes vínculos religiosos y relacionales entre los hermanos de fe.

  15. Don Quijote ja ranskalaiset säveltäjät

    OpenAIRE

    Orvo, Marko

    2012-01-01

    Tämä kirjallinen osuus opinnäytetyöstäni käsittelee Jacques Ibertin ja Maurice Ravelin laulusarjoja Don Quijotesta. Laulut sävellettiin venäläiselle bassolle Fjodor Shaljapinille Don Quijotesta kertovaa elokuvaa varten. Kerron tässä kirjallisessa osassa myös tunnelmia konsertin harjoitteluvaiheesta ja itse konsertista. Laulujen tekstit olen kääntänyt ja ne löytyvät liitteistä. Lisäksi olen tutkinut laulujen rakennetta ja tyylilajia. Olen myös liittänyt tiivistelmät säveltäjien elämäkerroi...

  16. On Test Data Compression Using Selective Don't-Care Identification

    Institute of Scientific and Technical Information of China (English)

    Terumine Hayashi; Haruna Yoshioka; Tsuyoshi Shinogi; Hidehiko Kita; Haruhiko Takase

    2005-01-01

    This paper proposes an effective method for reducing test data volume under multiple scan chain designs. The proposed method is based on reduction of distinct scan vectors using selective don't-care identification. Selective don't-care identification is repeatedly executed under condition that each bit of frequent scan vectors is fixed to binary values (0 or 1). Besides, a code extension technique is adopted for improving compression efficiency with keeping decompressor circuits simple in the manner that the code length for infrequent scan vectors is designed as double of that for frequent ones. The effectiveness of the proposed method is shown through experiments for ISCAS'89 and ITC'99 benchmark circuits.

  17. Cell Theory, Specificity, and Reproduction, 1837–1870

    OpenAIRE

    Müller-Wille, Staffan

    2010-01-01

    The cell is not only the structural, physiological, and developmental, but also the reproductive unit of life. So far, however, this aspect of the cell has received little attention by historians and philosophers of biology. I will argue that cell theory had far-reaching consequences for how biologists conceptualized the reproductive relationships between germs and adult organisms. Cell theory, as formulated by Theodor Schwann in 1839, implied that this relationship was a specific and lawful ...

  18. Reproduction et Diversité Génétique chez Parkia biglobosa (Jacq.) G. Don

    OpenAIRE

    Sina, S.

    2006-01-01

    Parkiabiglobosa(Jacq.)G.Don, African locust bean, is one of the 34 known species of the genusParkiawhose centre of origin isSouth America. It is anagroforestrytree species spread throughout the Africansudaniansavannas, fromSenegalin the West toUgandain the East. P.biglobosa is also an important socio-economic species in high demand by local communities for its multiple amenities and uses (food, traditional medicine and pharmacy, preservation and improvement of soil fertility, rites and custom...

  19. The perception of plants in the complete works of Cervantes, particularly “Don Quijote”

    OpenAIRE

    Morales Valverde, Ramón; Pardo de Santayana, Manuel; Tardío, Javier

    2006-01-01

    We searched the complete works of Cervantes, as historical ethnobotanical resources, for all references to plants, plant communities and products. Our analysis of the perception and uses of plants in Spain 400 years ago covers wild trees and shrubs; cultivated plants in fields and gardens, mainly edible species; medicinal plants; plants with technological applications; and ornamental and symbolic plants. 167 species were registered in the 38 works, 110 cited in “Don Quijote”. The ...

  20. Public Concern About Radiation: Why Our Fears Don't Match the Facts

    International Nuclear Information System (INIS)

    The problem about radioactive contamination of recycled materials is, at its heart, an issue of perception and emotion. This paper explains how the scientific disciplines of neuroscience and psychology can explain the way we perceive risk and why our fears of radiation and many other risks don't seem to match the facts. Finally, it explains the discipline of risk communication, and its use for addressing the problem of public concern about radiological issues. (author)

  1. Review: Don't Go Up Kettle Creek: Verbal Legacy of the Upper Cumberland

    OpenAIRE

    1984-01-01

    In Don't Go Up Kettle Creek, Montell reconstructs the history of the Upper Cumberland River region "as it is perceived from the vernacular point of view, relying on personal reminiscences, oral traditions, balladry and song, and printed materials (which were themselves derived from oral history data) as primary sources of information" (p. 1). Although these oral sources provide the substance of the book, Montell corroborates the oral information wherever possible using more standard historica...

  2. Don Quixote Intends to give us Music; and Being his it will not be Bad

    OpenAIRE

    Leonardo Sancho

    2015-01-01

    The Ingenious Gentleman Don Quixote of La Mancha is crossed with a quantity and variety of poems that result being eco of the events that happen in the narration. In this opportunity the romance is analyzed and commented. «Mighty Love the hearts of maidens», of chapter XLVI of the Second Part, is an example of the Cervantes poetics of love and cavalry that weaves relationships with other events, themes and poems, inserted in the Cervantes novel.

  3. Don Quixote Intends to give us Music; and Being his it will not be Bad

    Directory of Open Access Journals (Sweden)

    Leonardo Sancho

    2015-11-01

    Full Text Available The Ingenious Gentleman Don Quixote of La Mancha is crossed with a quantity and variety of poems that result being eco of the events that happen in the narration. In this opportunity the romance is analyzed and commented. «Mighty Love the hearts of maidens», of chapter XLVI of the Second Part, is an example of the Cervantes poetics of love and cavalry that weaves relationships with other events, themes and poems, inserted in the Cervantes novel.

  4. El mito de Don Juan en el cine: De Molière a Jacques Weber

    OpenAIRE

    Becerra Suárez, Carmen

    2011-01-01

    From its birth in Spain in the 17th century, the myth of Don Juan has been explored through a diversity of genres, approached by numerous artists, from a wide range of perspectives and artistic expressions, interpreted by philosophers and studied by scientists. This article analyzes the modifications that the mythic scenario displays in Jacques Weber’s cinematographic version, taking into account the specific issues related to the shift of language, from literature to cinema, alongside other ...

  5. Cosmogenic 7Be in ground level air in Rostov-on-Don (Russia) (2001-2011)

    CERN Document Server

    Buraeva, E A; Nephedov, V C; Shramenko, B I; Stasov, V V; Zorina, L V

    2013-01-01

    The deposition flux of cosmogenic 7Be in the industrial city Rostov-on-Don (Russia) from 2001 to 2011 has been measured. The variations of annual 7Be deposition flux appear to be mainly correlated with the number of the meteorological parameters and solar activity. For the first time correlations of the volume activity of cosmogenic 7Be with such meteorological parameters as temperature, precipitation, wind speed, atmospheric pressure, relative humidity are identified.

  6. The Manifestation of Presential Space in Don Berry’s Trask

    OpenAIRE

    Jörgne, Ella Therése

    2012-01-01

    The essay is a phenomenological study of Don Berry’s novel Trask (1960), demonstrating that experiential priority is given in the text to what Erwin Straus and Otto Bollnow call presential [präsentisch] space. The investigation analyses the difference between two forms of such space. On the one hand presential space is constituted automatically. This happens for the mountain man through the relentless momentum of long-distance trekking. On the other hand presential space is constituted as a ...

  7. A new combination in Orophea (Annonaceae for Uvaria nitida Roxb. ex G. Don

    Directory of Open Access Journals (Sweden)

    Ian M. Turner

    2014-12-01

    Full Text Available The identity of Uvaria nitida Roxb. ex G.Don. (Annonaceae has not been considered for 180 years. The plant is only known from material grown in the Calcutta Botanic Garden in India following introduction from, reportedly, the Moluccas. Examination of a specimen from the Brussels Herbarium, designated here as lectotype, indicates that the species is a member of Orophea subgenus Sphaerocarpon, similar to Orophea gabra Merr. A new combination in Orophea is made. 

  8. Advantages and disadvantages of franchise business opportunities in Rostov-on-don

    Directory of Open Access Journals (Sweden)

    S.A. Bugayan

    2013-09-01

    Full Text Available In  this  article  are  considered  main  cons  and  pros  of  franchise  business  opportunities  in  Rostov-on-Don,  using  different  businesses  as  an example,efficiency of this type of business and its future possibilities.

  9. Reproduction et Diversité Génétique chez Parkia biglobosa (Jacq.) G. Don

    NARCIS (Netherlands)

    Sina, S.

    2006-01-01

    Parkiabiglobosa(Jacq.)G.Don, African

  10. New observations of chemically peculiar stars with ESPaDOnS

    CERN Document Server

    Khalack, V; Thibeault, C; LeBlanc, F

    2015-01-01

    We present the first results of the estimation of gravity and effective temperature for some poorly studied chemically peculiar stars that were recently observed with the spectropolarimeter ESPaDOnS at CFHT. We have analyzed the spectra of HD71030, HD95608 and HD116235 to determine their radial velocity, Vsin(i) and the average abundance of several chemical species. We have also analyzed our results to verify for possible vertical abundance stratification of iron and chromium in these stars.

  11. Enterovirus A71 Meningoencephalitis Outbreak, Rostov-on-Don, Russia, 2013

    OpenAIRE

    Akhmadishina, Ludmila V.; Govorukhina, Marina V.; Kovalev, Evgeniy V.; Nenadskaya, Svetlana A.; Ivanova, Olga E.; Lukashev, Alexander N.

    2015-01-01

    Seventy-eight cases of enterovirus infection, including 25 neuroinfections, occurred in Rostov-on-Don, Russia, during May–June 2013. The outbreak was caused by an enterovirus A type 71 (EV-A71) subgenotype C4 lineage that spread to neighboring countries from China ≈3 years earlier. Enterovirus associated neuroinfection may emerge in areas with a preceding background circulation of EV-A71 with apparently asymptomatic infection.

  12. Isolation and Characterization of Antineoplastic Alkaloids from Catharanthus Roseus L. Don. Cultivated in Egypt

    OpenAIRE

    Shams, Khaled A; Nazif, Naglaa M; Abdel Azim, Nahla S; Abdel Shafeek, Khaled A; El-Missiry, Mostafa M.; Ismail, Shams I; Seif El Nasr, Medhat M

    2009-01-01

    Vinblastine and vincristine (the antileukemic agents) were isolated, in a pure form, from Catharanthus roseus L. Don., cultivated in Egypt, by several chromatographic techniques. Five modified methods for the preparation of total alkaloids were carried out. All the isolated mixtures were evaluated by HPLC and HPTLC analyses. The antineoplastic alkaloids; vinblastine and vincristine, were isolated by the use of vacuum liquid chromatographic column on silica gel : aluminium oxide (1:1) mixed be...

  13. Effect of Salinity Stress on Seed Germination Catharanthus roseus Don. Cvs. Rosea and Alba

    OpenAIRE

    Zahra Rezaee; Mehrangiz Chehrazi; Norollah Moalemi

    2012-01-01

    This experiment was carried out aiming to determine the Catharanthus roseus Don resistance against salinity, due to the increasing salinity of soil, and the importance of this plant as an ornamental flower, as well as the little information available on its tolerance against salinity during the germination period. In order to an experiment was conducted in randomized completely design factorial. Sodium chloride was used for induce salinity stress. These factors include cultivar and salinity l...

  14. Migration of Near Earth Asteroid to Jovian-Crosser Asteroid:Case Study 3552 Don Quixote

    OpenAIRE

    Siregar, Suryadi

    2010-01-01

    It is generally recognized that main-belt asteroids (MBAs) and nuclei of extinct comets are the two main sources for the Near-Earth-Asteroids (NEAs). Theoretical studies of NEAs dynamics and numerical modelling of their orbital motions showed that the resonance mechanism for supplying NEAs is quite sufficient to sustain this population. Asteroid 1983 SA, also known as 3552 Don Quixote, is one of Near Earth Asteroids (NEAs) and the most probable candidates for NEAs of the cometary origin. In t...

  15. Lunar influence on Brunfelsia uniflora (Pohl.) D. Don (manacá-de-cheiro) rooting.

    OpenAIRE

    Luciana Leal; Michelle Melissa Althaus Ottmann; Katia Christina Zuffellato-Ribas; Marisa de Cacia Oliveira; Áurea Portes Ferriani

    2012-01-01

    This study aimed to determinate the moon phase’s influence on Brunfelsia uniflora (Pohl.) D. Don. In January 2008, semi woody cuttings were collected in Curitiba, Brazil. The experiment was conducted in a randomly design with four treatments that represent the moon phases (new; full; waning and waxing). The cuttings were maintained inside a greenhouse. After 90 days the followings were analyzed: rooting percentage, number of roots, length of the three longer roots, un-rooted living cuttings, ...

  16. Detsibill : Meie Mees kui ANTI. White Label : Don Erikson. Kuula / DJ Pickney Tiger

    Index Scriptorium Estoniae

    DJ Pickney Tiger, pseud., 1970-

    2006-01-01

    Heliplaatidest: Don Erikson "Israel Mix", Congotronics 2 "Buzz'n'rumble from the urb'n'jungle", Critikal "Chapter One ehk Teine maitse", Nirvana "Silver, The best of the Box", Singer Vinger "Eesti Kullafond", Bullet Train To Vegas "We Put Scissors Where Our Mouths Are", Ludachris "Disturbing tha peace", "Soul Satisfaction 5", The Kurt Webber's Pink Band "Café Regatt", Patrizio Buanne "The Italian", Eminem "Curtain Call"

  17. Application of gamma radiation to the nodes detection in Pinus Radiata (D.Don) wood pieces

    International Nuclear Information System (INIS)

    Attenuation of 59.5 KeV photons provided by an Am-241 source, has been used to detect knots in lumber pieces from Pinus Radiata (D.Don). It is shown that the linear attenuation coefficient is a sensitive parameter to detect singularities in the structure of this material. The scanning of the piece provides profiles which define the position and extension of the singularity. (author)

  18. Effects of temperature and time on deoxynivalenol (DON and zearalenone (ZON content in corn

    Directory of Open Access Journals (Sweden)

    Jauković Marko

    2014-01-01

    Full Text Available Fumonisins are Fusarium mycotoxins that occur in corn and corn-based foods and they have been implicated in several animal and human diseases. Their effect on human health is unclear, however, fumonisins are considered to be risk factors for cancer. Baking, frying, and extrusion cooking of corn at high temperatures (190°C reduce fumonisin concentrations in foods, with the amount of reduction achieved depending on cooking time, temperature, recipe, and other factors. The aim of this work was to evaluate the effectiveness of temperature (200 and 220 °C and time (15 and 20 min on the detoxification of corn flour deliberately contaminated with DON and ZON. After processing at 200°C for 15 min, an average of 12% and after 20 min an average of 15% of DON was lost. At 200°C ZON content was reduced by 22% (after 15 min and by 27% (after 20 min. Higher temperature (220°C did not significantly affect further reduction of DON or ZON content. The process was only partially effective in both cases. [Projekat Ministarstva nauke Republike Srbije, br. TR-31023 i br. TR-31053

  19. The silent Don Juan. The concepts of love and art in the works of Max Frisch

    Directory of Open Access Journals (Sweden)

    Špela Virant

    2012-07-01

    Full Text Available In the novel Stiller and in the play Don Juan, both published in the early 1950s, Frisch already focuses on the problem of identity as one of his major themes, but it is not yet situated in a political context as, for example, in the play Andorra. In these works, the identity-problem is situated in the domain of intimate relationships and artistic creativity, offering the possibility to discuss Frisch’s concepts of art and love, which unfold through Frisch’s treatment of the traditional literary figure of Don Juan and his hubris. Frisch’s Stiller and Don Juan do not believe in God; but their frame of mind stays the same, while the position of God and his attributes are assigned to love, art and geometry. Although both literary figures in the course of events realize that this substitution is a mistake, they are unable to alter their concepts of love and art. Instead of altering the concepts, they rather resign in silence, while Max Frisch continues to search for alternatives in reflecting on the process of fictionalization and in developing innovative narrative and dramatic structures.

  20. MANUSCRITOS TRADUCTORIOS DEL BRASIL IMPERIAL. “LA ARAUCANA” DE DON PEDRO II

    Directory of Open Access Journals (Sweden)

    Ana María Barrera Conrad Sackl

    2014-06-01

    Full Text Available Resumen: Don Pedro II, emperador del Brasil entre 1840 y 1889, se dedicó con ahínco y pasión a la tarea traductora de varios idiomas hacia el portugués. Del español menciona en sus diarios dos trabajos, una traducción de "Granada" (1853 de Zorrilla, cuyo manuscrito aún no se ha encontrado y "La Araucana" de Don Alonso De Ercilla y Zúñiga (1558, que analizamos. El prototexto del último fue descubierto por el equipo de NUPROC (http://www.item.ens.fr/ en el IGHB de Rio de Janeiro en 2011, está fechado en 1889 y lleva la firma del emperador. El análisis se realiza desde la perspectiva teórica de la Crítica Genética y de los Estudios Descriptivos de la Traducción. Palabras claves: traducción; español; portugués; manuscritos de Don Pedro II. Manuscritos traduzidos no Brasil Imperial: La Araucana de Dom Pedro II Resumo: Don Pedro II, imperador do Brasil entre 1840 e 1889, dedicou-se com afinco e paixão à tarefa tradutória de vários idiomas para o português. Menciona em seus diários duas traduções do espanhol, Granada (1853 de Zorrilla, cujo manuscrito ainda não foi localizado e La Araucana de Dom Alonso de Ercilla y Zuñiga (1558 que analisamos neste artigo. O prototexto do último formado por três páginas foi descoberto pela equipe do NUPROC no IGBH de Rio de Janeiro em 2011, está assinada pelo imperador no ano de 1889. Os fundamentos teóricos da análise são a Crítica Genética e os Estudos Descritivos da Tradução. Palavras-chave: Tradução. Espanhol. Português. Manuscritos de Dom Pedro II. Translated manuscripts in Imperial Brasil: Don Pedro II's La Araucana Abstract: Don Pedro II, Emperor of Brazil between 1840 and 1889, devoted himself with zeal and passion to the task of translation into Portuguese texts from different languages. For his translation into Spanish he mentions in his diaries two works, a translation of Granada (1853 by Zorrilla, whose manuscript has not yet been found and La Araucana by Don

  1. A neural model for transient identification in dynamic processes with 'don't know' response

    International Nuclear Information System (INIS)

    This work presents an approach for neural network based transient identification which allows either dynamic identification or a 'don't know' response. The approach uses two 'jump' multilayer neural networks (NN) trained with the backpropagation algorithm. The 'jump' network is used because it is useful to dealing with very complex patterns, which is the case of the space of the state variables during some abnormal events. The first one is responsible for the dynamic identification. This NN uses, as input, a short set (in a moving time window) of recent measurements of each variable avoiding the necessity of using starting events. The other one is used to validate the instantaneous identification (from the first net) through the validation of each variable. This net is responsible for allowing the system to provide a 'don't know' response. In order to validate the method, a Nuclear Power Plant (NPP) transient identification problem comprising 15 postulated accidents, simulated for a pressurized water reactor (PWR), was proposed in the validation process it has been considered noisy data in order to evaluate the method robustness. Obtained results reveal the ability of the method in dealing with both dynamic identification of transients and correct 'don't know' response. Another important point studied in this work is that the system has shown to be independent of a trigger signal which indicates the beginning of the transient, thus making it robust in relation to this limitation

  2. Change and persistence in land surface phenologies of the Don and Dnieper river basins

    International Nuclear Information System (INIS)

    The formal collapse of the Soviet Union at the end of 1991 produced major socio-economic and institutional dislocations across the agricultural sector. The picture of broad scale patterns produced by these transformations continues to be discovered. We examine here the patterns of land surface phenology (LSP) within two key river basins-Don and Dnieper-using AVHRR (Advanced Very High Resolution Radiometer) data from 1982 to 2000 and MODIS (Moderate Resolution Imaging Spectroradiometer) data from 2001 to 2007. We report on the temporal persistence and change of LSPs as summarized by seasonal integration of NDVI (normalized difference vegetation index) time series using accumulated growing degree-days (GDDI NDVI). Three land cover super-classes-forest lands, agricultural lands, and shrub lands-constitute 96% of the land area within the basins. All three in both basins exhibit unidirectional increases in AVHRR GDDI NDVI between the Soviet and post-Soviet epochs. During the MODIS era (2001-2007), different socio-economic trajectories in Ukraine and Russia appear to have led to divergences in the LSPs of the agricultural lands in the two basins. Interannual variation in the shrub lands of the Don river basin has increased since 2000. This is due in part to the better signal-to-noise ratio of the MODIS sensor, but may also be due to a regional drought affecting the Don basin more than the Dnieper basin.

  3. Turner syndrome: don't forget the vulva

    OpenAIRE

    Haidopoulos, Dimitrios; Bakolas, George; Michala, Lina

    2016-01-01

    Summary Turner syndrome (TS) has been linked to a number of autoimmune conditions, including lichen sclerosus (LS), at an estimated prevalence of 17%. LS is a known precursor to vulvar cancer. We present a case of vulvar cancer in a 44-year-old woman, who had previously complained of pruritus in the area, a known symptom of LS. Histology confirmed a squamous cell carcinoma with underlying LS. Vulvar assessment for the presence of LS should be undertaken regularly as part of the routine assess...

  4. Turner syndrome: don't forget the vulva

    Science.gov (United States)

    Haidopoulos, Dimitrios; Bakolas, George

    2016-01-01

    Summary Turner syndrome (TS) has been linked to a number of autoimmune conditions, including lichen sclerosus (LS), at an estimated prevalence of 17%. LS is a known precursor to vulvar cancer. We present a case of vulvar cancer in a 44-year-old woman, who had previously complained of pruritus in the area, a known symptom of LS. Histology confirmed a squamous cell carcinoma with underlying LS. Vulvar assessment for the presence of LS should be undertaken regularly as part of the routine assessments proposed for adult TS women. If LS is identified, then the patient should be warned of the increased risk of vulvar cancer progression and should be monitored closely for signs of the condition. Learning points Patients with TS are at increased risk of developing LS.LS is a known precursor to vulvar cancer.TS women with LS may be at risk of developing vulvar cancer and should be offered annual vulvar screening and also be aware of signs and symptoms of early vulvar cancer. PMID:27252865

  5. c-Myb in incisor stem cell niches and in differentiation of odontogenic cells

    Czech Academy of Sciences Publication Activity Database

    Matalová, Eva; Lungová, Vlasta; Buchtová, Marcela; Švandová, Eva; Šmarda, J.

    Warwick: BSCB, 2012. 93-94. [British Society for Developmental Biology, Bristish Society for Cell Biology and Japanese Society of Developmental Biologists Joint Spring Meeting. 15.04.2012-18.04.2012, Warwick] R&D Projects: GA ČR GCP302/12/J059; GA ČR GAP304/11/1418 Institutional support: RVO:67985904 Keywords : c-Myb Subject RIV: EA - Cell Biology

  6. Don Quijote de la Mancha: Reflexiones en torno a las relaciones cine-literatura Don Quijote de la Mancha: Reflections about the relationships between cinematography-literature

    Directory of Open Access Journals (Sweden)

    Felipe Ardila Rojas

    2008-06-01

    Full Text Available Este artículo presenta un análisis de algunos aspectos de la relación cine-literatura, a partir del Don Quijote de La Mancha literario y de algunas versiones cinematográficas y televisivas realizadas en español y en inglés. Inicialmente se presentan problemas teóricos en torno a categorías que suelen serles afines: relato, autor, narración, historia, etc., para involucrar alternativamente fenómenos que, desde el punto de vista de los autores, generan ambigüedades textuales o múltiples interpretaciones en las versiones que se analizan. De este análisis comparativo que parte de los relatos en cuestión se llega finalmente a conclusiones de carácter estético e ideológico sobre los mismos.This article presents an analysis of some aspects of the relationship between cinematography and literature. Starting from the Don Quijote de La Mancha and taking into account some film and television versions developed in Spanish and English. At the beginning, a theoretical problem based on similar types for concepts categorization: author, narration, story, relat, among others will be analyzed. In addition, a connection is made with some authors' concepts in order to avoid textual ambiguity or multiple interpretations in the versions that are analyzed. Through a comparative analysis supported in these stories, it is possible to reach some aesthetic and ideological conclusions about them.

  7. Regional economic impacts of current and proposed management alternatives for Don Edwards National Wildlife Refuge

    Science.gov (United States)

    Richardson, Leslie; Huber, Chris; Koontz, Lynne

    2012-01-01

    The National Wildlife Refuge System Improvement Act of 1997 requires all units of the National Wildlife Refuge System to be managed under a Comprehensive Conservation Plan. The Comprehensive Conservation Plan must describe the desired future conditions of a Refuge and provide long-range guidance and management direction to achieve refuge purposes. The Don Edwards San Francisco Bay National Wildlife Refuge, located at the south end of California's San Francisco Bay and one of seven refuges in the San Francisco Bay National Wildlife Refuge Complex, is in the process of developing a range of management goals, objectives, and strategies for the Comprehensive Conservation Plan. The Comprehensive Conservation Plan must contain an analysis of expected effects associated with current and proposed Refuge management strategies. For Refuge Comprehensive Conservation Plan planning, a regional economic analysis provides a means of estimating how current management (No Action Alternative) and proposed management activities (alternatives) affect the local economy. This type of analysis provides two critical pieces of information: (1) it illustrates the Don Edwards San Francisco Bay National Wildlife Refuge's contribution to the local community, and (2) it can help in determining whether economic effects are or are not a real concern in choosing among management alternatives. This report first presents a description of the local community and economy near the Don Edwards San Francisco Bay National Wildlife Refuge. Next, the methods used to conduct a regional economic impact analysis are described. An analysis of the final Comprehensive Conservation Plan management strategies that could affect stakeholders, residents, and the local economy is then presented. The management activities of economic concern in this analysis are: * Spending in the local community by Refuge visitors; * Refuge personnel salary spending; and * Refuge purchases of goods and services within the local

  8. El arzobispado de Don Gonzalo de Aguilar en Santiago de Compostela (1348-1351)

    OpenAIRE

    Sánchez Sánchez, Xosé M.

    2010-01-01

    In the middle of the 14th century the prelature of Santiago de Compostela is occupied by Don Gonzalo de Aguilar, archbishop transferred from Sigüenza and whose pontificate is extended between 1348 and 1351, when he is transferred to Toledo. He is a figure that remained until now in the complete darkness of the history of the Compostellan church, disappearing in fact, in many cases, of the historical considerations, that they avoided him or simply they did not have noticed of his figure, exten...

  9. Stokes IQUV Magnetic Doppler Imaging of Ap stars I. ESPaDOnS and NARVAL Observations

    OpenAIRE

    Silvester, J.; Wade, G. A.; Kochukhov, O.; Bagnulo, S.; Folsom, C. P.; Hanes, D.

    2012-01-01

    In this paper we describe and evaluate new spectral line polarisation observations obtained with the goal of mapping the surfaces of magnetic Ap stars in great detail. One hundred complete or partial Stokes IQUV sequences, corresponding to 297 individual polarised spectra, have been obtained for 7 bright Ap stars using the ESPaDOnS and NARVAL spectropolarimeters. The targets span a range of mass from approximately 1.8 to 3.4 solar mass, a range of rotation period from 2.56 to 6.80 days, and a...

  10. Don Bosco, animator za zvanja. Povijesna razmišljanja za salezijanski pastoral zvanja

    OpenAIRE

    Canino Zanoletty, Miguel

    2011-01-01

    Neprijateljsko okruženje koje je često obilježavalo europsku panoramu u 19. st. imalo je teške posljedice na promicanje zvanja. U razdoblju u kojemu se uopće nije razvijao sustavni pastoral zvanja, don Bosco se osjetio posebno ponukanim da djelotvorno odgovori na takvo stanje. Iako nije bio prvi, njegova osoba i stil nizom su ga pojedinosti učinile nadaleko priznatim animatorom za zvanja. Uspio je oduševiti i privuči brojne osobe u odgojno poslanje i evangelizaciju mladeži. U svom djelovan...

  11. El sistema preventivo de Don Bosco, respuesta a la escuela multicultural

    OpenAIRE

    Arenal Jorquera, María José

    2009-01-01

    El objetivo de este estudio es estudiar si el Sistema Preventivo de Don Bosco, que se lleva en todos los centros salesianos de los cinco continentes, da respuesta a la escuela multicultural actual. Y esto se produce como consecuencia de que las escuelas salesianas hacen realidad un medio escolar intercultural basado en el paradigma holístico, que se adapta al contexto de cada momento histórico en tiempo y espacio. El punto de partida del mencionado sistema educativo surge en la realidad s...

  12. From Don Quixote to The Tick: The Reflection of Sancho Panza in the Comic Book Sidekick

    OpenAIRE

    Annacondia, José Manuel

    2012-01-01

    Esta Memoria de Licenciatura explora la relación entre Sancho Panza, el escudero de Don Quijote en la novela cervantina, y los "sidekicks" del cómic de superhéroes, trazando su linage desde los comienzos de la literatura hasta nuestros días, pasando por los ejemplos más tradicionales de este arquetipo de personaje, los creados en la Edad de Oro del cómic estadounidense y las últimas tendencias en la deconstrucción del medio.

  13. Don DeLillo: Mao II / Underworld / Falling Man. Ed. Stacey Olster.

    Directory of Open Access Journals (Sweden)

    Angeliki Tseti

    2011-10-01

    Full Text Available The latest volume of the Continuum Studies in Contemporary North American Fiction is dedicated to three of the most influential recent novels written by Don DeLillo: Mao II, Underworld and Falling Man. These novels are set in pivotal moments of recent history, the end of the Cold War, the millennium and 9/11 and, so, offer fertile ground for the investigation of themes, such as terrorism, consumerism and globalism, that are dominant in the author’s entire oeuvre; what is more, the contributio...

  14. Is the Partner the Don Quixote of the 21st Century Corporate World?

    DEFF Research Database (Denmark)

    Bévort, Frans; Poulfelt, Flemming

    2015-01-01

    for change. This raises the question of whether the Partner ownership and management model is a relic of the past - and the partner a Don Quixote-figure of the 21st century corporate world? Based on an ethnographic study of a PSF in Denmark, and data from other sources, the paper analyzes the changing...... the future of the partner role in complex organizations. Finally, the paper proposes that professional partnership needs to find a ' new balance' between the traditional professional logic of the partners and the still more influential logic of bureaucracy of the modern complex organization to...

  15. Chemical Composition and Possible in Vitro Phytotoxic Activity of Helichrsyum italicum (Roth Don ssp. italicum

    Directory of Open Access Journals (Sweden)

    Vincenzo De Feo

    2011-09-01

    Full Text Available The chemical composition of the essential oil of Helichrysum italicum (Roth Don ssp. italicum, collected in the National Park of Cilento and Diano Valley, Southern Italy, was studied by means of GC and GC/MS. Forty four compounds of 45 constituents were identified in the oil, mainly oxygenated sesquiterpenes. The essential oil was evaluated for its potential in vitro phytotoxic activity against germination and early radicle elongation of radish and garden cress. The radicle elongation of radish was significantly inhibited at the highest doses tested, while germination of both seeds was not affected.

  16. Chemical composition and possible in vitro phytotoxic activity of Helichrsyum italicum (Roth) Don ssp. italicum.

    Science.gov (United States)

    Mancini, Emilia; De Martino, Laura; Marandino, Aurelio; Scognamiglio, Maria Rosa; De Feo, Vincenzo

    2011-01-01

    The chemical composition of the essential oil of Helichrysum italicum (Roth) Don ssp. italicum, collected in the National Park of Cilento and Diano Valley, Southern Italy, was studied by means of GC and GC/MS. Forty four compounds of 45 constituents were identified in the oil, mainly oxygenated sesquiterpenes. The essential oil was evaluated for its potential in vitro phytotoxic activity against germination and early radicle elongation of radish and garden cress. The radicle elongation of radish was significantly inhibited at the highest doses tested, while germination of both seeds was not affected. PMID:21904272

  17. Tolerances don't lie: or how to tolerance an optical system so that it works

    International Nuclear Information System (INIS)

    There has been much written over the years about the generation of optical system fabrication, assembly, and alignment tolerances leading to a performance error budget and predicted system performance. Over my 40 years of optical design experience I have become more cautious (and concerned) about how accurately we, the optics community, are tolerancing our systems and predicting their manufactured performance. In this paper I will share with you some of these concerns along with recommended ways of avoiding the many speed bumps, pot holes and other common issues encountered when tolerancing. And of course I will reveal why tolerances don't lie

  18. Survey of genome sequences in a wild sweet potato, Ipomoea trifida (H. B. K.) G. Don

    OpenAIRE

    Hirakawa, Hideki; Okada, Yoshihiro; Tabuchi, Hiroaki; Shirasawa, Kenta; Watanabe, Akiko; Tsuruoka, Hisano; Minami, Chiharu; Nakayama, Shinobu; Sasamoto, Shigemi; Kohara, Mitsuyo; Kishida, Yoshie; Fujishiro, Tsunakazu; Kato, Midori; Nanri, Keiko; Komaki, Akiko

    2015-01-01

    Ipomoea trifida (H. B. K.) G. Don. is the most likely diploid ancestor of the hexaploid sweet potato, I. batatas (L.) Lam. To assist in analysis of the sweet potato genome, de novo whole-genome sequencing was performed with two lines of I. trifida, namely the selfed line Mx23Hm and the highly heterozygous line 0431-1, using the Illumina HiSeq platform. We classified the sequences thus obtained as either ‘core candidates’ (common to the two lines) or ‘line specific’. The total lengths of the a...

  19. Body Control in American Cyberspace: A Study on Don Delillo’s White Noise

    OpenAIRE

    Ruzbeh Babaee; Wan Roselezam Bt Wan Yahya; Ida Baizura Binti Bahar

    2014-01-01

    Don DeLillo’s White Noise (1984) is a cyberpunk narrative commenting on dystopia of late capitalism by illustrating the instability of the apparently safe and perfect life of a small town in North America. The dystopian society in white Noise is not a hierarchal world or a world in another time and space, as it is common in most dystopian narratives. It is a world that we may experience in our daily life. This study attempts to demonstrate that DeLillo criticizes the condition of the late twe...

  20. Glutamine analogs promote cytoophidium assembly in human and Drosophila cells

    Institute of Scientific and Technical Information of China (English)

    Kangni Chen; Jing Zhang; (O)mür Yilmaz Tastan; Zillah Anne Deussen; Mayte Yu-Yin Siswick; Ji-Long Liu

    2011-01-01

    CTP synthase is compartmentalized within a subcellular structure,termed the cytoophidium,in a range of organisms including bacteria,yeast,fruit fly and rat.Here we show that CTP synthase is also compartmentalized into cytoophidia in human cells.Surprisingly,the occurrence of cyloophidia in human cells increases upon treatment with a glutamine analog 6-diazo-5-oxo-L-norleucine (DON),an inhibitor of glutaminedependent enzymes including CTP synthase.Experiments in flies confirmned that DON globally promotes cytoophidium assembly.Clonal analysis via CTP synthase RNA interference in somatic cells indicates that CTP synthase expression level is critical for the formation of cytoophidia.Moreover,DON facilitates cytoophidium assembly even when CTP synthase level is low.A second glutamine analog azaserine also promotes cytoophidum formation.Our data demonstrate that glutamine analogs serve as useful tools in the study of cytoophidia.

  1. The fusarium mycotoxin deoxynivalenol can inhibit plant apoptosis-like programmed cell death.

    Directory of Open Access Journals (Sweden)

    Mark Diamond

    Full Text Available The Fusarium genus of fungi is responsible for commercially devastating crop diseases and the contamination of cereals with harmful mycotoxins. Fusarium mycotoxins aid infection, establishment, and spread of the fungus within the host plant. We investigated the effects of the Fusarium mycotoxin deoxynivalenol (DON on the viability of Arabidopsis cells. Although it is known to trigger apoptosis in animal cells, DON treatment at low concentrations surprisingly did not kill these cells. On the contrary, we found that DON inhibited apoptosis-like programmed cell death (PCD in Arabidopsis cells subjected to abiotic stress treatment in a manner independent of mitochondrial cytochrome c release. This suggested that Fusarium may utilise mycotoxins to suppress plant apoptosis-like PCD. To test this, we infected Arabidopsis cells with a wild type and a DON-minus mutant strain of F. graminearum and found that only the DON producing strain could inhibit death induced by heat treatment. These results indicate that mycotoxins may be capable of disarming plant apoptosis-like PCD and thereby suggest a novel way that some fungi can influence plant cell fate.

  2. MARQUES DE L’AFFECTIVITÉ DANS LE DISCOURS SÉDUCTEUR DU DON JUAN DE MOLIÈRE

    OpenAIRE

    Diana-Adriana LEFTER

    2007-01-01

    Notre travail prend en discussion l’un des plus célèbres libertins de l’histoire littéraire, Don Juan, tel qu’il est repris dans la pièce de Molière. Plus précisément, nous nous proposons d’étudier le discours de séduction de Don Juan dans les différentes hypostases dans lesquelles il apparaît. Nous voulons mettre en parallèle le discours de Don Juan devant Done Elvire, et celui devant la paysanne Charlotte, pour mettre en évidence deux aspects: la composante affective, c’est-à-dire l’inserti...

  3. Gender differences in national assessment of educational progress science items: What does i don't know really mean?

    Science.gov (United States)

    Linn, Marcia C.; de Benedictis, Tina; Delucchi, Kevin; Harris, Abigail; Stage, Elizabeth

    The National Assessment of Educational Progress Science Assessment has consistently revealed small gender differences on science content items but not on science inquiry items. This assessment differs from others in that respondents can choose I don't know rather than guessing. This paper examines explanations for the gender differences including (a) differential prior instruction, (b) differential response to uncertainty and use of the I don't know response, (c) differential response to figurally presented items, and (d) different attitudes towards science. Of these possible explanations, the first two received support. Females are more likely to use the I don't know response, especially for items with physical science content or masculine themes such as football. To ameliorate this situation we need more effective science instruction and more gender-neutral assessment items.

  4. Don Yllán and the Egyptian Sorcerer: Vernacular commonality and literary diversity in medieval Castile

    Directory of Open Access Journals (Sweden)

    Wacks, David A.

    2005-12-01

    Full Text Available In this article the author compares the exemplo of Don Yllán and the Deán de Santiago -no. 11 in Don Juan Manuel's Conde Lucanor (ca. 1335- with an earlier Hebrew analogue found in the Hebrew Mešal Haqadmonī (ca. 1285 of fellow Castilian author Isaac ibn Sahula. A thorough analysis of the rhetorical and narrative style of both versions reveals that the two tales shared a common source in Castilian oral tradition. The appearance of the tale in an earlier Hebrew text from Castile (the only other known version in any language calls into question the originality of Don Juan Manuel's most famous exemplo, suggesting a productive interplay between a common oral tradition in Castilian and coexisting literary traditions in Hebrew and Castilian.

    En este artículo, el autor compara el exemplo de Don Yllán y el Deán de Santiago -n.° 11 en el Conde Lucanor (ca. 1335 de Don Juan Manuel- con una versión anterior en la obra hebrea, Mešal Haqadmonī (ca. 1285 de otro autor castellano, Isaac ibn Sahula. Un análisis cuidadoso del estilo narrativo y retórico de ambas versiones revela que las dos comparten una fuente común en la tradición oral castellana. La aparición del cuento en un texto hebreo anterior de Castilla (la única versión conocida en cualquier otra lengua cuestiona la originalidad literaria del más famoso exemplo de Don Juan Manuel, y sugiere un intercambio productivo entre una tradición oral común castellana y tradiciones literarias hebreas y castellanas coexistentes.

  5. La teoría clásica del don y la donación de sangre

    Directory of Open Access Journals (Sweden)

    Casado Neira, David

    2003-04-01

    Full Text Available The Gift Theory exposed by Marcel Mauss is here used to analyze free, not paid and universal blood donation. According to Mauss, every gift involves three obligations: to give, to get and to return. All of them are present in modern blood donation, even gift is still in force as social exchange. But the gift of blood acquires some specific characteristics and, even when it does not follow the conventional patterns of a gift, it is not possible to obviate its validity. Some problems and conflicts generated under this biomedical practice would be understood if attention to the fuzzy persistence of the logic of gift were played, also in strongly institutionalized social contexts.

    La teoría del don enunciada por Marcel Mauss es en esta ocasión utilizada para analizar la donación de sangre (voluntaria, no remunerada y universal. Las tres obligaciones que implica el don (dar, recibir y devolver no están ausentes en la moderna donación de sangre, así como tampoco lo está la vigencia del don como forma de intercambio social; pero en la transfusión de sangre el don adquiere unas características específicas que, si bien no nos permite considerar esta donación como una forma convencional de don, tampoco es posible obviar su vigencia. Parte de los problemas y conflictos generados en torno a esta práctica biomédica no pueden ser comprendidos si no se presta atención a la difusa persistencia del don en contextos sociales fuertemente institucionalizados.

  6. The Influence of Processing by Impulse Pressure on the Productivity of the Don Barley (Hordeum vulgare L.

    Directory of Open Access Journals (Sweden)

    Pavlova Violetta Aleksandrovna

    2014-04-01

    Full Text Available Plant productivity is the important indicator, which determines the amount of yield. The productivity of plants depends on the number of bruchids per plant and on the weight of 1000 bruchids. The article studies the influence of impulse pressure of various magnitudes on plant productivity of Don barley (Hordeum vulgare L.. It was found that the pressure of 17 MPa was the most effective for increasing the productivity. Impulse pressure of other magnitudes also had influence on the productivity of Don barley.

  7. Growth performance, serum biochemical profile, jejunal morphology, and the expression of nutrients transporter genes in deoxynivalenol (DON)- challenged growing pigs

    OpenAIRE

    Li WU; Liao, Peng; He, Liuqin; Ren, Wenkai; Yin, Jie; Duan, Jielin; Li, Tiejun

    2015-01-01

    Background Fusarium infection with concurrent production of deoxynivalenol (DON) causes an increasing safety concern with feed worldwide. This study was conducted to determine the effects of varying levels of DON in diets on growth performance, serum biochemical profile, jejunal morphology, and the differential expression of nutrients transporter genes in growing pigs. Results A total of twenty-four 60-day-old healthy growing pigs (initial body weight = 16.3 ± 1.5 kg SE) were individually hou...

  8. De El burlador de Tirso al Don Juan de Molière. Estudio Comparativo de Fuentes Textuales

    OpenAIRE

    Ortiz Rosado, Irene

    2014-01-01

    He querido reflejar en este trabajo las fuentes o influencias folklóricas, literarias y de ciertos personajes nobles de la sociedad de la época, tanto de El burlador de Tirso de Molina como de Don Juan de Molière, además de realizar un estudio comparativo de ambas obras para analizar los elementos de convergencia o de desunión en una y otra comedia. De forma exhaustiva, he querido mostrar prácticamente todo lo que han escrito sobre el tema de don Juan expertos como Menéndez Pidal, Márquez ...

  9. Effect of storage conditions on post planting water status and performance of Pinus radiata D. Don stock-types

    OpenAIRE

    Mena-Petite, Amaia; Estavillo, José; Duñabeitia, Miren; González-Moro, Begoña; Muñoz-Rueda, Alberto; Lacuesta, Maite

    2004-01-01

    Effet des conditions de stockage sur l'état hydrique et l'établissement de plantules de Pinus radiata D. Don avec motte et à racines nues. L'étude a porté sur l'effet du stockage sur la qualité du plant et sur les potentialités de survie initiale de plantules de Pinus radiata D. Don à racine nue (BR) et avec motte (PR), replantées dans des sols humides et secs. Les plantules ont subi un stress de transplantation, même dans des conditions hydriques optimales. Malgré la diminution de la transpi...

  10. Patterns of dissolved organic carbon (DOC and nitrogen (DON fluxes in deciduous and coniferous forests under historic high nitrogen deposition

    Directory of Open Access Journals (Sweden)

    S. Sleutel

    2009-07-01

    Full Text Available Numerous recent studies have indicated that dissolved organic carbon (DOC and nitrogen (DON play an important role in C and N cycling in natural ecosystems, and have shown that N deposition alters the concentrations and fluxes of dissolved organic substances and may increase leaching losses from forests. Our study was set up to accurately quantify concentrations and flux patterns of DOC, DON and dissolved inorganic nitrogen (DIN in deciduous and coniferous forest in Flanders under historical high nitrogen deposition. We measured DOC, DON and DIN concentrations at two weekly intervals in a silver birch (SB stand, a corsican pine (CP stand and a pine stand with higher N deposition (CPN, and used the SWAP model (calibrated with PEST for generating accurate water and matter fluxes. The input with precipitation was an important source of DON, but not for DOC. Release of DOC from the forest floor was minimally affected by forest type, but higher N deposition (CPN stand caused an 82% increase of DOC release from the forest floor. Adsorption to mineral soil material rich in iron and/or aluminum oxyhydroxides was suggested to be the most important process removing DOC from the soil solution, responsible for substantial retention (67–84% of DOC entering the mineral soil profile with forest floor leachate. Generally, DON was less reactive (i.e. less removal from the soil solution than DOC, resulting in decreasing DOC/DON ratios with soil depth. We found increased DOC retention in the mineral soil as a result of higher N deposition (84 kg N ha−1 yr−1 additional DOC retention in CPN compared to CP. Overall DON leaching losses were 2.2, 3.3 and 5.0 kg N ha−1 yr−1 for SB, CP and CPN, respectively, contributing between 9–28% to total dissolved N (TDN leaching. DON loss from SB and CP was not much higher than from unpolluted forests, and its relative contribution to TDN leaching was mainly determined by

  11. Ecfrasis literaria y arquitectura novelesca: Los cuadernos de don Rigoberto de Mario Vargas Llosa

    Directory of Open Access Journals (Sweden)

    Pedro Antonio Agudelo

    2013-07-01

    Full Text Available Este artículo propone una relectura de la novela Los cuadernos de don Rigoberto de Mario Vargas Llosa, concentrando la atención en la figura retórica de la ecfrasis. Examina la arquitectura novelesca y la representación verbal y visual como pilares de lo que constituiría una genuina novela iconoverbal. El estudio está soportado en las estrategias de análisis provenientes de la semiótica literaria, la narratología y la retórica, que permiten una comprensión amplia del objeto de estudio. This paper proposes a different interpretation of the novel Los cuadernos de don Rigoberto by Mario Vargas Llosa, focusing on the rhetorical figure of ekphrasis. It examines the novelistic architecture and verbal and visual representation as pillars of what would constitute a genuine iconoverbal novel. The study is supported by the analysis strategies from literary semiotics, narratology and rhetoric, which allow a comprehensive understanding of subject matter.

  12. Confirmation of Don Borghi's experiment on the synthesis of neutrons from protons and electrons

    CERN Document Server

    Santilli, R M

    2006-01-01

    Following Rutherford's 1920 historical hypothesis of the neutron as a compressed hydrogen atom in the core of stars, the laboratory synthesis of the neutron from protons and electrons was claimed in the late 1960 by the Italian priest-physicist Don Carlo Borghi and his associates via a metal chamber containing a partially ionized hydrogen gas at a fraction of $1 bar$ pressure traversed by an electric arc with $5 J$ energy and microwaves with $10^{10} s^{-1}$ frequency. The experiment remained unverified for decades due to the lack of theoretical understanding of the results. In this note we report various measurements showing that, under certain conditions, electric arcs within a hydrogen gas produce neutral, hadron-size entities that are absorbed by stable nuclei and subsequently result in the release of detectable neutrons, thus confirming Don Borghi's experiment. The possibility that said entities are neutrons is discussed jointly with other alternatives. Due to their simplicity, a primary scope of this no...

  13. Influence of microstructured carbon materials on Sequoia sempervirens (D. Don. Endl. in vitro culture

    Directory of Open Access Journals (Sweden)

    Raluca Stoiculescu

    2009-12-01

    Full Text Available The present experimental research has been mainly focused on Sequoia sempervirens (D. Don. Endl. as woody plant model system, for testing the influence of some microstructured carbon materials (CCM comparing with the activated charcoal action, on the in vitro growth. The work hypothesis was that CCM can induce effects on the plant culture, similar to those induced by activated charcoal, due to their high absorption capacity. The influence of two types CCM and activated charcoal introduced in the medium culture was evaluated on the fresh weight and on the length of the in vitro regenerants, after 30 and 60 days of cultivation. Our data revealed that the CCM is not toxic and does not inhibit plant growth. We also remarked that S. sempervirens (D. Don. Endl. was more reactive to the activated charcoal into the nutritive medium than the CCM compounds. The use of these new microstructured compounds is an important step both in the study of materials science and in the field of plant biotechnologies.

  14. Influence of microstructured carbon materials on Sequoia sempervirens (D. Don. Endl. in vitro culture

    Directory of Open Access Journals (Sweden)

    Raluca Stoiculescu

    2009-11-01

    Full Text Available The present experimental research has been mainly focused on Sequoiasempervirens (D. Don. Endl. as woody plant model system, for testing the influence of some microstructured carbon materials (CCM comparing with the activated charcoal action, on the in vitro growth. The work hypothesis was that CCM can induce effects on the plant culture, similar to those induced by activated charcoal, due totheir high absorption capacity. The influence of two types CCM and activated charcoal introduced in the medium culture was evaluated on the fresh weight and on the length of the in vitro regenerants, after 30 and 60 days of cultivation. Our data revealed that the CCM is not toxic and does not inhibit plant growth. We also remarked that S. sempervirens (D. Don. Endl. was more reactive to the activatedcharcoal into the nutritive medium than the CCM compounds. The use of these new microstructured compounds is an important step both in the study of materials science and in the field of plant biotechnologies.

  15. Don't play the butter notes’: jazz in medical education

    Science.gov (United States)

    Bradner, Melissa; Harper, Darryl V.; Ryan, Mark H.; Vanderbilt, Allison A.

    2016-01-01

    Jazz has influenced world music and culture globally – attesting to its universal truths of surviving, enduring, and triumphing over tragedy. This begs the question, what can we glean in medical education from this philosophy of jazz mentoring? Despite our training to understand disease and illness in branching logic diagrams, the human experience of illness is still best understood when told as a story. Stories like music have tempos, pauses, and silences. Often they are not linear but wrap around the past, future, and back to the present, frustrating the novice and the experienced clinician in documenting the history of present illness. The first mentoring lesson Hancock discusses is from a time he felt stuck with his playing – his sound was routine. Miles Davis told him in a low husky murmur, ‘Don't play the butter notes’. In medical education, ‘don't play the butter notes’ suggests not undervaluing the metacognition and reflective aspects of medical training that need to be fostered during the early years of clinical teaching years. PMID:27095009

  16. 'Don't play the butter notes': jazz in medical education.

    Science.gov (United States)

    Bradner, Melissa; Harper, Darryl V; Ryan, Mark H; Vanderbilt, Allison A

    2016-01-01

    Jazz has influenced world music and culture globally - attesting to its universal truths of surviving, enduring, and triumphing over tragedy. This begs the question, what can we glean in medical education from this philosophy of jazz mentoring? Despite our training to understand disease and illness in branching logic diagrams, the human experience of illness is still best understood when told as a story. Stories like music have tempos, pauses, and silences. Often they are not linear but wrap around the past, future, and back to the present, frustrating the novice and the experienced clinician in documenting the history of present illness. The first mentoring lesson Hancock discusses is from a time he felt stuck with his playing - his sound was routine. Miles Davis told him in a low husky murmur, 'Don't play the butter notes'. In medical education, 'don't play the butter notes' suggests not undervaluing the metacognition and reflective aspects of medical training that need to be fostered during the early years of clinical teaching years. PMID:27095009

  17. Leakage Current Optimization Techniques During Test Based on Don't Care Bits Assignment

    Institute of Scientific and Technical Information of China (English)

    Wei Wang; Yu Hu; Yin-He Han; Xiao-Wei Li; You-Sheng Zhang

    2007-01-01

    It is a well-known fact that test power consumption may exceed that during functional operation. Leakage power dissipation caused by leakage current in Complementary Metal-Oxide-Semiconductor (CMOS) circuits during test has become a significant part of the total power dissipation. Hence, it is important to reduce leakage power to prolong battery life in portable systems which employ periodic self-test, to increase test reliability and to reduce test cost. This paper analyzes leakage current and presents a kind of leakage current simulator based on the transistor stacking effect.Using it, we propose techniques based on don't care bits (denoted by Xs) in test vectors to optimize leakage current in integrated circuit (IC) test by genetic algorithm. The techniques identify a set of don't care inputs in given test vectors and reassign specified logic values to the X inputs by the genetic algorithm to get minimum leakage vector (MLV).Experimental results indicate that the techniques can effectually optimize leakage current of combinational circuits and sequential circuits during test while maintaining high fault coverage.

  18. Defining Hair Follicles in the Age of Stem Cell Bioengineering

    OpenAIRE

    Chuong, Cheng-Ming; Cotsarelis, George; Stenn, Kurt

    2007-01-01

    One challenge faced by stem cell biologists is the bioengineering of an organ. Ehama et al. (2007, this issue) used cells derived from human and rodent epidermis and dermal papilla to reconstitute hair-follicle mini-organs. Some result in hair follicles; others are hair follicle–like. The challenge calls for the development of a set of criteria to define a hair follicle so that bioengineered products in the future can be evaluated.

  19. Single-cell resolution of morphological changes in hemogenic endothelium

    OpenAIRE

    Bos, FL; Hawkins, JS; Zovein, AC

    2015-01-01

    © 2015. Published by The Company of Biologists Ltd. Endothelial-to-hematopoietic transition (EHT) occurs within a population of hemogenic endothelial cells during embryogenesis, and leads to the formation of the adult hematopoietic system. Currently, the prospective identification of specific endothelial cells that will undergo EHT, and the cellular events enabling this transition, are not known. We set out to define precisely the morphological events of EHT, and to correlate cellular morphol...

  20. Generation and analysis of expressed sequence tags from six developing xylem libraries in Pinus radiata D. Don

    Directory of Open Access Journals (Sweden)

    Dillon Shannon K

    2009-01-01

    Full Text Available Abstract Background Wood is a major renewable natural resource for the timber, fibre and bioenergy industry. Pinus radiata D. Don is the most important commercial plantation tree species in Australia and several other countries; however, genomic resources for this species are very limited in public databases. Our primary objective was to sequence a large number of expressed sequence tags (ESTs from genes involved in wood formation in radiata pine. Results Six developing xylem cDNA libraries were constructed from earlywood and latewood tissues sampled at juvenile (7 yrs, transition (11 yrs and mature (30 yrs ages, respectively. These xylem tissues represent six typical development stages in a rotation period of radiata pine. A total of 6,389 high quality ESTs were collected from 5,952 cDNA clones. Assembly of 5,952 ESTs from 5' end sequences generated 3,304 unigenes including 952 contigs and 2,352 singletons. About 97.0% of the 5,952 ESTs and 96.1% of the unigenes have matches in the UniProt and TIGR databases. Of the 3,174 unigenes with matches, 42.9% were not assigned GO (Gene Ontology terms and their functions are unknown or unclassified. More than half (52.1% of the 5,952 ESTs have matches in the Pfam database and represent 772 known protein families. About 18.0% of the 5,952 ESTs matched cell wall related genes in the MAIZEWALL database, representing all 18 categories, 91 of all 174 families and possibly 557 genes. Fifteen cell wall-related genes are ranked in the 30 most abundant genes, including CesA, tubulin, AGP, SAMS, actin, laccase, CCoAMT, MetE, phytocyanin, pectate lyase, cellulase, SuSy, expansin, chitinase and UDP-glucose dehydrogenase. Based on the PlantTFDB database 41 of the 64 transcription factor families in the poplar genome were identified as being involved in radiata pine wood formation. Comparative analysis of GO term abundance revealed a distinct transcriptome in juvenile earlywood formation compared to other stages of

  1. Las Voces de la Tradicion en "Don Segundo Sombra" a la Luz de la Reflexion Estilistica de Amado Alonso

    OpenAIRE

    Martos Nuñez, Eloy

    1995-01-01

    SE EXPLICA EL PROBLEMA DE LA SINGULARIDAD ESTILISTICA DE DON SEGUNDO SOMBRA, A PARTIR DEL ANALISIS DE AMADO ALONSO. GÜIRALDES OPTO POR LA FORMULA DE UNAS MEMORIAS Y POR CREAR UN LENGUAJE NUEVO QUE INTEGRASE LA RICA PERSPECTIVA DE LA TRADICION Y SUS NARRADORES, Y SU PROPIA VISION PLASTICA Y LITERARIA.

  2. Social and Political Activization of the Don Nobility in the Early 20th Century and “Noble Liberalism”

    Directory of Open Access Journals (Sweden)

    Bratolyubova Mariya Viktorovna

    2014-03-01

    Full Text Available Peculiarity of the social, economic and legal status of the Don nobility influenced its political alertness and the opposition character of activity. The liberal wing supported the creation of a new world outlook system which was expected to provide the basis for transforming the political system by means of gradual transition to people’s representatives or constitutional form of government. The liberal landowners insisted on the introduction of zemstvo (district council, the solution of the Cossack issue; they strived for liberalization of the military and bureaucratic control of the Cossack troops and demanded the withdrawal of civil government agencies from the competence of the Military department. The liberal nobility actively supported the change of conditions of service for the Cossacks and pressed for easier draft obligation and releasing the Cossack troops from police duties which clashed with the military dignity. The conservative type of liberalism prevailed within the noble class of the DHR that distanced itself from both the left and the right political forces. The liberalism of the Don nobles was also a reflection of social and cultural peculiarities of the Cossacks. The social basis of the Don liberalism differed from the common Russian analogs. As the Cossack class was rather numerous at the Don, and its representatives made up quite an important part of the regional liberal corporation, they influenced its tactics and the political programming considerably.

  3. Antioxidant and anticholinesterase investigations of Rumex hastatus D. Don: potential effectiveness in oxidative stress and neurological disorders

    OpenAIRE

    2015-01-01

    Background Rumex species are traditionally used for the treatment of neurological disorders including headache, migraine, depression, paralysis etc. Several species have been scientifically validated for antioxidant and anticholinestrase potentials. This study aims to investigate Rumex hastatus D. Don crude methanolic extract, subsequent fractions, saponins and flavonoids for acetylcholinestrase, butyrylcholinestrase inhibition and diverse antioxidant activities to validate its folkloric uses...

  4. Don't Call Them Dropouts: Understanding the Experiences of Young People Who Leave High School before Graduation

    Science.gov (United States)

    Hynes, Michelle

    2014-01-01

    "Don't Call Them Dropouts" adds to the large and growing body of research about why some young people fail to complete high school on the traditional four-year timeline. While a high school diploma is only a starting line for adult success, it has become increasingly clear that it is crucial for taking the next steps in college and…

  5. Sarmatian Monuments in the Forest-Steppe Interfluve Area Between the Don and the Volga Rivers (Zoning Experience

    Directory of Open Access Journals (Sweden)

    Berestnev Roman Sergeevich

    2015-06-01

    Full Text Available The article is devoted to the zoning of Sarmatian antiquities in the forest-steppe interfluve area between the Don and the Volga rivers. On the basis of the analysis, the authors allocate four areas of burial monuments in the Northern periphery of the Asian Sarmatia (the river in Voronezh, the Middle Don, the Upper Don, the area between the Khoper and the Volga rivers. The stages of the forest-steppe area development by the Sarmatians in the Don River, the Khoper river and the right bank of the Volga river were also studied. The first stage is characterized by the general description of Sarmatian monuments located in the forest-steppe area between the Volga and the Khoper. The authors carry out the detailed review of the distinctive features of the funeral rites and some peculiar details: stratigraphy, structures beneath barrows, forms of burial pits, postures of the deceased, orientation, use of fire, imported and domestic utensils, weapons and ornaments. Archaeological materials are compared with the data of ancient sources (Claudius Ptolemy. The result of the study consists in the comparative analysis of the binding of specific ethnic groups in the allocated areas. The authors attribute the Sarmatians on the Voronezh river with the Gippofagi ancient authors; the Aseyes and the Sakata – with the area of monuments in the forest between the Volga and the Khoper.

  6. Synthetic Biology Outside the Cell: Linking Computational Tools to Cell-Free Systems

    OpenAIRE

    Lewis, Daniel D.; Villarreal, Fernando D.; Wu, Fan; Tan, Cheemeng

    2014-01-01

    As mathematical models become more commonly integrated into the study of biology, a common language for describing biological processes is manifesting. Many tools have emerged for the simulation of in vivo synthetic biological systems, with only a few examples of prominent work done on predicting the dynamics of cell-free synthetic systems. At the same time, experimental biologists have begun to study dynamics of in vitro systems encapsulated by amphiphilic molecules, opening the door for the...

  7. Fluorescence lifetime imaging microscopy (FLIM) to quantify protein-protein interactions inside cells.

    Science.gov (United States)

    Duncan, R R

    2006-11-01

    Recent developments in cellular imaging spectroscopy now permit the minimally invasive study of protein dynamics inside living cells. These advances are of interest to cell biologists, as proteins rarely act in isolation, but rather in concert with others in forming cellular machinery. Until recently, all protein interactions had to be determined in vitro using biochemical approaches: this biochemical legacy has provided cell biologists with the basis to test defined protein-protein interactions not only inside cells, but now also with high spatial resolution. These techniques can detect and quantify protein behaviours down to the single-molecule level, all inside living cells. More recent developments in TCSPC (time-correlated single-photon counting) imaging are now also driving towards being able to determine protein interaction rates with similar spatial resolution, and together, these experimental advances allow investigators to perform biochemical experiments inside living cells. PMID:17052173

  8. Una figura funeraria infantil de madera en el Instituto Valencia de Don Juan

    Directory of Open Access Journals (Sweden)

    Fernández Ahijado, Carmen

    2003-09-01

    Full Text Available Entre las piezas que componen la colección de escultura del Instituto Valencia de don Juan encontramos la estatua orante de un niño, realizada en madera, tela encolada y yeso. Sus medidas son 77,5 cm de alto, 38 cm de ancho y 38,5 cm de fondo. Presenta elegancia señorial, carácter y vitalidad en su cabeza. Aparece ante nuestros ojos con los mismos ademanes y la misma figura rígida y envarada que los adultos. La actitud recogida junto con su sencilla vestimenta acentúan el intimismo religioso del orante. Se consideró que era interesante encontrar otros ejemplos de este tipo de escultura funeraria infantil en madera, material poco usado en este género debido a su propia naturaleza. La madera se asociaba a transitoriedad…

  9. El político Don Fernando El Católico

    OpenAIRE

    Montaner Frutos, Alberto

    2001-01-01

    En la obra "El político Don Fernando El Católico" Baltasar Gracián cifra una reflexión política de hondo calado en un tomito en dieciseisavo que vio por primera vez la luz en 1640. En esta obra podemos encontra además de los problemas bibliográficos y ecdóticos, tres aspectos fundamentales podríamos considerar en el análisis de El Político: el estilístico, el histórico cuyo contenido desborda la fundamental referencia a Fernando el Católico que campea ya desde la portada; y el propia...

  10. El sistema preventivo de Don Bosco en la escuela del siglo XXI

    OpenAIRE

    Navajas-Josa, Alejandro

    2014-01-01

    Este trabajo pretende abordar el método preventivo de don Bosco (1815-1888), sacerdote y educador italiano, fundador de los salesianos, congregación religiosa que cuenta en la actualidad con casi 16.000 sacerdotes que operan en más de 130 países del mundo. En 1877, publicó un opúsculo donde recogía las líneas maestras de su metodología, que se sigue aplicando en los miles de colegios y centros de formación profesional de los salesianos. A lo largo de estas páginas se demuestra cómo este siste...

  11. Metasomatic processes within the fertile lithospheric Mantle beneath Don Camilo, Santa Cruz, Argentina

    Science.gov (United States)

    Ntaflos, Th.; Mundl, A.; Bjerg, E. A.; Tschegg, C.; Kosler, J.

    2009-04-01

    Mantle xenoliths in alkalibasalts from Don Camilo, an area located at the North margin of the Deseado Massif in Patagonia, comprise mainly spinel-bearing lherzolites and dunites as well as less common harburgites, wehrlites, clinopyroxenites and gabbros. Spinel-lherzolites and harzburgites have protogranular textures whereas dunites have equigranular to equigranular tabular textures. There are two kinds of dunites: mantle and cumulate dunites. The olivine mg# in the mantle dunites vary within a narrow range from 90.5 to 91.5 and the NiO content from, 0.39 to 0.42 wt. %, whereas in the cumulate dunites the mg# ranges from 87 to 90.5 and the NiO content from 0.22 to 0.40 wt. %. Both types of dunite contain fine grained interstitial diopside. Hydrous phases, besides one sample that contains amphibole, were so far not found. The spinel peridotites have Primitive Mantle normalized (PM) whole rock REE abundances depleted in LREE [(La/Yb)N=0.34-0.85)] and the dunites are LREE enriched [(La/Yb)N=3.49]. LA-ICP-MS analyses of cpx show that a number of the studied spinel peridotite xenoliths experienced cryptic metasomatism. Three groups of xenoliths have been recognized according to their PM normalized REE and other incompatible trace element patterns in cpx: group I has depleted LREE abundances and group II is highly enriched in LREE (20-30 x PM) and group III has moderate LREE enrichments. Cores of some clinopyroxenes in group II have depleted LREE similar to those in group I, apparently representing relictic cores not affected by metasomatism. In addition, the metasomatized clinopyroxenes are significantly enriched in Sr, Th and U. Evidently, the metasomatic agent was a H2O-rich fluid (high LREE, Sr, Th and U). Mantle and cumulate dunites have experienced modal metasomatism. In both types of dunite interstitial clinopyroxene appear to be of metasomatic origin. The clinopyroxene from cumulate dunites depleted LREE abundances and low HREE indicating that they have been

  12. "Gathering Facts for the End of the World": Don DeLillo's Archive of Global Turbulence

    Directory of Open Access Journals (Sweden)

    Cornelius Collins

    2016-05-01

    Full Text Available Over his career, Don DeLillo grows to inhabit more complex and critical ideas about politics, capitalism, and strategies for opposition than the remote affect of his earlier work might suggest. As part of this project, he developed an advanced analysis of conditions under the global finance regimes gaining strength in the West. The author's papers at the Harry Ransom Center show that he arrived at this informed position through a program of independent research, his newspaper cuttings from this period vividly demonstrating reflections on the meaning of US global power. Once back in New York, his investment in public intellectual debates on the meaning of "terrorism" led to a characteristic but problematic emphasis on free speech as a political issue. More recently, his work has turned to sophisticated theoretical texts for increasingly subtle ideas about globalization and its opponents.

  13. Compte rendu de "Les enfants de Don Quichotte", P. Bruneeaux, Presses Universitaires de Vincennes, 2013

    OpenAIRE

    Frère, Bruno

    2014-01-01

    Les enfants de Don Quichotte est un ouvrage qui présente intérêt non négligeable. Il s’agit de l’une des rares études sociologiques (la seule ?) portant sur un mouvement qui pourtant fit grand bruit dans le monde médiatique et politique il y a quelques années : l’occupation des abords du canal Saint Martin par des SDF regroupés sous tentes. C’est délibérément que les auteurs de ce collectif ont choisi l’optique de la sociologie critique bourdieusienne pour enquêter. À ce titre, le parti pris ...

  14. Don, obligation et reconnaissance dans les Lettres d’une Péruvienne de Graffigny

    Directory of Open Access Journals (Sweden)

    Olivier Delers

    2009-05-01

    Full Text Available « Il n’entrait jamais dans ma chambre sans m’offrir un présent de quelques-unes des choses merveilleuses dont cette contrée abonde » (Lettre 11, 69. Ces petits cadeaux que Zilia, la princesse péruvienne de Graffigny, reçoit régulièrement de son protecteur français Déterville seraient bien anodins si la relation entre les deux personnages principaux de ce roman épistolaire n’était fondée sur l’asymétrie du don. La générosité en apparence désintéressée de l’officier français, qui commence quan...

  15. [Genetic structure of the populations of Pallas pine (Pinus pallasiana D. Don) reforested in extreme conditions].

    Science.gov (United States)

    Korshikov, I I; Krasnoshtan, O V

    2010-01-01

    The paper reports on an intensive reforestation of Pallas pine (Pinus pallasiana D. Don) in post-fire sites in the native Mountainous Crimean populations and around the previously planted seed-producing trees in ore-mining dumps of the Krivoy Rog region. Self-sown progeny growing in the dump is characterized by a better growth and comes to the reproductive development phase earlier compared to that one growing in post-fire forest. Allele variability at 20 allozyme loci is less in self-sown progeny than in the native populations whereas its heterozygosity level is similar. Genetic distance (D(N)) among self-sown progenies in post-fire sites and ore-mining dump is comparable to that of the natural populations. PMID:20608157

  16. NH4NO3 fertilizer induces DON leaching from Sphagnum magellanicum

    Science.gov (United States)

    Hartsock, J. A.; Vile, M. A.; Wieder, R.

    2011-12-01

    Boreal peatlands globally function as long-term sinks for atmosheric CO2. Mosses in the genus Sphagnum dominate the understory of boreal peatlands, especially in ombrotrophic bogs for which the sole sources of new nitrogen (N) is atmospheric deposition and biological N2-fixation. Sphagnum mosses in undisturbed regions generally are thought to be N-limited because of slow nutrient turnover and low rates of atmospheric N deposition. In Alberta, Canada, mean background N deposition rates are less than 1 kg N ha-1yr-1, but increasing atmospheric N deposition is occurring as a consequence of oil sands development in northern Alberta. In some European countries, anthropogenic practices have increased annual N deposition rates to levels in excess of 20 kg N ha-1yr-1. Increased rates of atmospheric N deposition have been shown to alter N cycling processes of Sphagnum mosses. Current hypotheses propose that Sphagnum mosses leach dissolved organic nitrogen (DON) from their tissues to mitigate N saturation stress in high N deposition areas. The goal of this study was to determine whether Sphagnum magellanicum leaches DON when exposed to elevated concentrations of NH4NO3 applied experimentally as synthetic rainfall. Sphagnum magellanicum was collected from a fen in Driftwood, Pennsylvania (41°13'58"N 78°11'44"W). Intact cores (10.5cm x 5.5cm) were grown in 500 mL culture flasks in a controlled environment chamber at Villanova University. Ammonium nitrate was applied to moss treatments eight times over 73 days at concentrations equivalent to 6 kg N ha-1yr-1, 12 kg N ha-1yr-1, and 18 kg N ha-1yr-1 (n=5 replicates per treatment, 400 mL H2O per N addition). Leachates (freely drained water samples) were analyzed for dissolved total nitrogen (TN), ammonium (NH4+-N), nitrate (NO3--N), and DON (TN-DIN) concentrations. Results show that after 73 days, increasing DIN resulted in: 1) a shift from net N mineralization to net NH4+ immobilization (Control: 376.3 ± 15.2 ug/g; High N: -377

  17. Body Control in American Cyberspace: A Study on Don Delillo’s White Noise

    Directory of Open Access Journals (Sweden)

    Ruzbeh Babaee

    2014-01-01

    Full Text Available Don DeLillo’s White Noise (1984 is a cyberpunk narrative commenting on dystopia of late capitalism by illustrating the instability of the apparently safe and perfect life of a small town in North America. The dystopian society in white Noise is not a hierarchal world or a world in another time and space, as it is common in most dystopian narratives. It is a world that we may experience in our daily life. This study attempts to demonstrate that DeLillo criticizes the condition of the late twentieth century in which human beings are entrapped and become disembodied in cyberspace. This study also reveals that human beings live in a state of suspension in cyberspace. Furthermore, it is of great significance for this study to show that cyberspace is a control system that manipulates human beings through their bodies in the contemporary world.

  18. Orthodox Revival in the Lower Volga Area and the Don in 1942–1943

    Directory of Open Access Journals (Sweden)

    Mordvinov Sergey Valeryevich

    2013-02-01

    Full Text Available The paper shows the impact of the Battle of Stalingrad in the revival of Orthodoxy in the Lower Volga and the Don, a comparison of the reconstruction process in the dioceses of the region; its characteristics are revealed in Stalingrad, Saratov, Astrakhan and Rostov regions. The author comes to the conclusion that the battle of Stalingrad was the catalyst recovery Orthodoxy in the province. The pace of discovery of temples was higher in the occupied areas of the Rostov and Stalingrad areas than in front-line areas. Imbalance in the number of active parishes continued to persist after the end of the Battle of Stalingrad. Church patriotic charity in 1942–1943 was the most active in the Saratov and Stalingrad diocese.

  19. The Secret List of Dos and Don'ts for Filmmaking

    Science.gov (United States)

    Kramer, N.

    2012-12-01

    Science is a massive black box to billions of people who walk the streets. However, the process of filmmaking can be equally as mystifying. As with the development of many scientific experiments, the process starts on a napkin at a restaurant…but then what? The road to scientific publication is propelled by a canonical list of several dos and don't that fit most situations. An equally useful list exists for up-and-coming producers. The list streamlines efforts, optimizes your use of the tools at your fingertips and enhances impact. Many fundamentals can be learned from books, but during this talk we will project and discuss several examples of best practices, from honing a story, to identifying audience appeal, filming, editing and the secrets of inexpensively acquiring expert help. Whether your goal is a two-minute webisode or a 90 minute documentary, these time-tested practices, with a little awareness, can give life to your films.;

  20. Analysis of Several Popular Cultivars of Madagascar Periwinkle (Catharanthus roseus (L. G. Don. using Biochemical Markers

    Directory of Open Access Journals (Sweden)

    Owk ANIEL KUMAR

    2013-12-01

    Full Text Available Band designs of esterase (EST, peroxidase (PO and polyphenol oxidase (PPO isozymes in several selected cultivars of Catharanthus roseus by using native polyacrylamide gel electrophoresis (PAGE were investigated in this study. It was confirmed that cultivar differences in isozyme polymorphism can be revealed by applied electrophoretic patterns. Three isozyme systems produced a total of 16 bands with polymorphism ranged from 66.6-100%. Considering the patterns of isozyme variations in the five cultivars of Catharanthus roseus, it is evident that the cultivar ‘First kiss coral’ displayed crimson red petal with large white eye’ displayed demarked profiles of EST, PO and PPO isozymes than other cultivars. This is the first report on isozyme polymorphism in members of the Cathanarathus roseus (L. G. Don.

  1. What We Don't Understand, We Explain to Each Other

    Science.gov (United States)

    Pines, David

    2015-12-01

    "What we don't understand, we explain to each other" was Robert Oppenheimer's 1948 description of theoretical physics as a profession. Because the phrase connects research, teaching, and learning, it seemed the right approach for the talk I gave to the AAPT on receiving the 2013 J.D. Jackson Award for Excellence in Graduate Teaching, on which the present article is based. In what follows I describe how my experiences as a student, researcher, and teacher combined to shape the way I learn and teach physics, from my own courses to inventing and editing Frontiers in Physics, reshaping Reviews of Modern Physics, and experimenting with different approaches to communicating key concepts in physics.

  2. Dons quotidiens dans les espaces sacrés de la zawya

    OpenAIRE

    Naji, Salima

    2012-01-01

    L’office du Saint, anwal, est un vaste espace composé de plusieurs salles dont l’une possède un foyer allumé tous les matins. L’eau de pluie, don de Dieu recueilli par les nombreuses citernes qui entourent l’édifice, est portée à ébullition dans d’imposants chaudrons faisant couscoussiers, pour ces bontés systématiquement servies aux pèlerins. En effet, cette eau réputée pour ses qualités purificatrices, accompagne une assiette de couscous d’orge brut très salé, offerts à l’hôte de passage, c...

  3. Lunar influence on Brunfelsia uniflora (Pohl. D. Don (Manacá-de-cheiro rooting

    Directory of Open Access Journals (Sweden)

    Luciana Leal

    2011-12-01

    Full Text Available This study aimed to determinate the moon phase’s influence on Brunfelsia uniflora (Pohl. D. Don. In January 2008, semi woody cuttings were collected in Curitiba, Brazil. The experiment was conducted in a randomly design with four treatments that represent the moon phases (new; full; waning and waxing. The cuttings were maintained inside a greenhouse. After 90 days the followings were analyzed: rooting percentage, number of roots, length of the three longer roots, un-rooted living cuttings, dead cuttings and cuttings with sprouts. It was also evaluated the amount of reducing sugar and total sugar. From the results obtained it was not possible conclude that the moon phases had any influence on the rooting of Brunfelsia uniflora.

  4. SOCIAL MOVEMENTS OF VOLGA-DON REGION IN THE FIRST RUSSIAN REVOLUTION (1905-1907

    Directory of Open Access Journals (Sweden)

    N. F. Bolotov

    2016-01-01

    Full Text Available The article is an attempt to analyze and provide scientific assessment of the events of the political history of Russia in the early XX century, whiсh took place, in the opinion of the authors, under influence by a number of objective factors. The regional specific of the article (in the example of the southern districts of the Saratov province and Cossack areas of the Volga-Don interfluve aims to search for the general laws of the formation of social and political movements that aim to change the existing system in Russia during the First Russian Revolution. The authors attempt to systematize the vast amount of material, which allows to identify the main causes of the mass revolutionary movement, character and social composition of the political groups and their impact on changes in the socio-political life of the region. Particular attention is paid to the activities of the Russian Social-Democratic Party, which position was strengthened by difficult economic plight of the workers and repressive government policies aimed at curbing the revolutionary movement. In modern conditions of social and political instability in many regions of the world, provoked by political technologies of "orange" revolution, it is highly relevant to study history of the First Russian Revolution of 1905-1907, which the 110th anniversary passed unnoticed. Despite the defeat, the revolution was of great importance for the emergence of civil society in Russia. As a result of mass protests of various social strata of Russian society, the first steps towards the formation of constitutionalism and parliamentarianism in Russia were made. In predominantly peasant Volga-Don region with high industrialized city Tsaritsyn all the contradictions of feudal-capitalist Russia's reality were focused and were clearly manifested, that turned into a powerful social explosion, which became the preparation of the 1917 Revolution.

  5. Transcriptional profiling of endogenous germ layer precursor cells identifies dusp4 as an essential gene in zebrafish endoderm specification

    OpenAIRE

    Brown, Jamie L.; Snir, Mirit; Noushmehr, Houtan; Kirby, Martha; Hong, Sung-Kook; Elkahloun, Abdel G.; Feldman, Benjamin

    2008-01-01

    A major goal for developmental biologists is to define the behaviors and molecular contents of differentiating cells. We have devised a strategy for isolating cells from diverse embryonic regions and stages in the zebrafish, using computer-guided laser photoconversion of injected Kaede protein and flow cytometry. This strategy enabled us to perform a genome-wide transcriptome comparison of germ layer precursor cells. Mesendoderm and ectoderm precursors cells isolated by this method differenti...

  6. DMPD: How Toll-like receptors signal: what we know and what we don't know. [Dynamic Macrophage Pathway CSML Database

    Lifescience Database Archive (English)

    Full Text Available 16343886 How Toll-like receptors signal: what we know and what we don't know. O'Nei...like receptors signal: what we know and what we don't know. PubmedID 16343886 Title How Toll-like receptors signal: what... we know and what we don't know. Authors O'Neill LA. Publication Curr...ll LA. Curr Opin Immunol. 2006 Feb;18(1):3-9. Epub 2005 Dec 15. (.png) (.svg) (.html) (.csml) Show How Toll-

  7. Peut-on penser le travail par le don ? Débat avec la rédaction de la Nouvelle Revue du Travail

    OpenAIRE

    Caillé, Alain; Alter, Norbert

    2012-01-01

    Dans un échange avec la Nouvelle Revue du Travail, Alain Caillé et Norbert Alter soutiennent leur lecture maussienne des réalités contemporaines du travail en discutant les questions centrales du capitalisme, du salariat, de l’entreprise et du travail sociologique. Cet échange fait suite aux contributions d’Alain Caillé et Norbert Alter dans ce Chorus « Peut-on penser le travail par le don ? De la mise en œuvre du paradigme du don contre-don en sociologie du travail. »

  8. Cell biology as the basis of a better understanding of cancer

    OpenAIRE

    Wheatley Denys N

    2005-01-01

    Abstract Clinicians will argue that cancer can only really receive the treatment that is needed through thorough understanding of medicine. However, even empirical approaches to therapy result in experimental analysis of the agencies involved on test cells, usually in culture. From the obverse perspective, cell biologists will argue that until we fully understand cell cycle regulation, tumour management will be too imprecise to make the best advances. A forum is needed whereby the fundamental...

  9. Effects of Pinus flexilis on the dynamics and structure of plant communities on the northern Rocky Mountain front, and, Training biologists for emerging niches in non-traditional jobs

    Science.gov (United States)

    Baumeister, Dayna Marie

    been characterized by invasion of P. flexilis and subsequent shifts in understory species composition apparently facilitated by P. flexilis. Lastly, I examined the role of the emerging science of biomimicry in providing employment for biologists. Biomimicry as a discipline seeks design solutions inspired by nature. Industries employing this tool require biologists with an understanding of biology from a functional perspective, good communication skills, and a background in applied natural history.

  10. Amendment to refuge hunting plan for waterfowl on 5,500 acres of former commercial salt ponds : Don Edwards San Francisco Bay NWR

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This amendment to the 1982 Don Edwards San Francisco Bay NWR waterfowl hunting plan was written to address 5,500 acres of salt pond recently acquisitioned by the...

  11. El mito de Don Quijote en la novela victoriana: The Newcomes, de William Thackeray, y The Ordeal of Richard Feverel, de George Meredith

    OpenAIRE

    Pardo García, Pedro Javier

    2008-01-01

    [ES]El presente articulo trata sobre la influencia que el personaje Don Quijote de Miguel de Cervantes tiene en la novela victoriana. Explorando el “cervantismo” en las obra de George Meredith y de William Makepeace Thackeray.

  12. On the "I don't think..." and "I think...not..." constructions%"I don't think..."和"I think...not..."的句式分析

    Institute of Scientific and Technical Information of China (English)

    方立

    2002-01-01

    本文探讨"I don't think..."和"I think...not…"这两个句式的句法、语义和语用,以及可用于这两个句式的词类.分析表明,此句式可用否定词提升转换规则解释,但该规则只适用于非事实谓词的一个小类.

  13. MENINGKATKAN MOTIVASI DAN PRESTASI BELAJAR MATEMATIKA SISWA KELAS V A SD FRATER DON BOSCO TARAKAN MENGGUNAKAN MODEL PEMBELAJARAN KOOPERATIF TIPE TEAM ASISTED INDIVIDUALIZATION TAHUN PELAJARAN 2010/2011

    OpenAIRE

    Agustinus Toding Bua

    2011-01-01

    "Agustinus Toding Bua. Meningkatkan Motivasi dan Prestasi Belajar Siswa Kelas V A SD Frater Don Bosco Tarakan Menggunakan Model Pembelajaran Kooperatif Tipe TAI Tahun Pelajaran 2010/2011 (dibimbing oleh Samsudin dan Jero). Tujuan Penelitian Untuk meningkatkan motivasi dan prestasi belajar matematika siswa kelas V A SD frater Don Bosco Tarakan pada materi mengidentifikasi sifat-sifat bangun datar dan bangun ruang. Pengumpulan data diperoleh dari hasil observasi, angket motivasi, dan pos tes p...

  14. Karakterisasi Simplisia Dan Skrining Fitokimia Serta Isolasi Alkaloida Dari Ekstrak Metanol Daun Kitolod (Hippobroma Longiflora (L.) G. Don)

    OpenAIRE

    Lubis, Suci Lestari

    2015-01-01

    Kitolod (Hippobroma longiflora (L.) G. Don) is an aromatic plant of the family Campanulaceae. The purpose of this research was to determine the simplex characteristics, and to determine the group of chemical compounds contained in kitolod leaves and the isolation of alkaloidal compounds. The isolates were characterized by ultraviolet (UV) and infrared (IR) spectrophotometries. This research included simplex characterization, phytochemical screening, extraction by percolation with methanol...

  15. Proteomic profiling of proteins associated with the rejuvenation of Sequoia sempervirens (D. Don) Endl

    OpenAIRE

    Chen Yu-Ting; Kuo Ching-I; Huang Bau-Lian; Hsu Ya-Wen; Hsieh Tsung-Ju; Shen Chin-Hui; Chen Peng-Jen; Chang Ing-Feng; Chu Hsiu-An; Yeh Kai-Wun; Huang Li-Chun

    2010-01-01

    Abstract Background Restoration of rooting competence is important for rejuvenation in Sequoia sempervirens (D. Don) Endl and is achieved by repeatedly grafting Sequoia shoots after 16 and 30 years of cultivation in vitro. Results Mass spectrometry-based proteomic analysis revealed three proteins that differentially accumulated in different rejuvenation stages, including oxygen-evolving enhancer protein 2 (OEE2), glycine-rich RNA-binding protein (RNP), and a thaumatin-like protein. OEE2 was f...

  16. MENINGKATKAN HASIL BELAJAR SISWA PADA PEMBELAJARAN IPA MENGGUNAKAN METODE EKSPERIMEN DI KELAS IV B SD FRATER DON BOSCO TARAKAN

    OpenAIRE

    Katarina Gaudentia Radjo

    2011-01-01

    KATARINA GAUDENTIA RADJO. 2011. Meningkatkan Hasil Belajar Siswa pada Pembelajaran IPA Menggunakan Metode Eksperimen di Kelas IV B SD Frater Don Bocso Tarakan. Skripsi. Program Studi Pendidikan Guru Sekolah Dasar Fakultas Keguruan dan Ilmu Pendidikan Universitas Borneo Tarakan. Dimbing oleh: Herdiansya dan Arief Ertha Kusuma. Penelitian ini memakai model Penelitian Tindakan Kelas (PTK) yang dilaksanakan oleh guru untuk memecahkan masalah-masalah pembelajaran yang dihadapi oleh guru dan untuk...

  17. Evaluation of phytochemical content, antimicrobial, cytotoxic and antitumor activities of extract from Rumex hastatus D. Don roots

    OpenAIRE

    Sahreen, Sumaira; Khan, Muhammad Rashid; Khan, Rahmat Ali; Hadda, Taibi Ben

    2015-01-01

    Background Being a part of Chinese as well as ayurdic herbal system, roots of Rumex hastatus D. Don (RH) is highly medicinal, used to regulated blood pressure. It is also reported that the plant is diuretic, laxative, tonic, used against microbial skin diseases, bilious complaints and jaundice. The present study is conducted to evaluate phytochemical, antimicrobial, antitumor and cytotoxic activities of extract obtained from R. hastatus roots. Methods RH roots were powdered and extracted with...

  18. Hypoglycaemic Effects of Methanolic Leaf Extract of Catharanthus Roseus (LINN.) G. DON (Apocynaceae) in Normal and Diabetic Mice

    OpenAIRE

    Ojewole, John A O; Adewunmi, Clement O

    2000-01-01

    In Southern Africa, as in many other parts of Africa, Catharanthus roseus (Linn.) G. Don is traditionally used in forklore medicine to treat diabetes and a number of other human ailments. Consequently, the hypoglycaemic effects of methanolic leaf extract of C. roseus have been investigated in streptozotocin (STZ)-induced diabetic (hyperglycaemic) mice as well as in normal (normoglycaemic), STZ-untreated mice. Tolbutamide has been used as reference hypoglycaemic agent. While tolbutamide produc...

  19. The class specific effects of compliance training with "do" and "don't" requests: analogue analysis and classroom application.

    OpenAIRE

    Neef, N A; Shafer, M S; Egel, A L; Cataldo, M F; Parrish, J M

    1983-01-01

    Two experiments are reported in which the relationship between compliance with "do" and "don't" requests was examined with developmentally disabled children. In Experiment 1, a multiple baseline design across subjects with counterbalanced treatment conditions was used to evaluate a compliance training program composed of four phases: (a) baseline, during which no consequences were delivered for compliance, (b) reinforcement for compliance with one targeted "do" request, (c) reinforcement for ...

  20. Fate of Fusarium mycotoxins in cereal product supply chain: the DON case within industrial bread-making technology

    OpenAIRE

    Bergamini, Elena; Catellani, Dante; Dall'Asta, Chiara; Galaverna, Gianni; Dossena, Arnaldo; Marchelli, Rosangela; Suman, Michele

    2010-01-01

    Abstract Fusarium mycotoxins are a relevant problem in the cereal supply chain at a worldwide level, being wheat, maize and barley the main contaminated crops; mould growth could happen in the pre-harvest phase and also during transport and storage due to ineffective drying conditions. Among Fusarium toxins, deoxynivalenol (DON) is considered the most important contaminant in wheat, due to its wide-spread occurrence. In the last years the European Food Safety Authority and the Euro...

  1. Hellig handling, grusom gjerning - en studie av menneskeofringsmotivet og -tematikken i Goethes Iphigenie auf Tauris og Schillers Don Karlos

    OpenAIRE

    Saxegaard, Jostein

    2009-01-01

    In dieser Masterarbeit werden Menschenopfermotive und -thematik in Johann Wolfgang Goethes Iphigenie auf Tauris (1787) und Friedrich Schillers Don Karlos. Infant von Spanien. (1787) untersucht. Im ersten Kapitel wird das Projekt präsentiert und begründet. Danach wird im zweiten Kapitel die Literaturgeschichte des Menschenopfermotivs dargestellt um die vielen thematischen Möglichkeiten des Motivs anzudeuten. Auf der Grundlage theoretischer Arbeiten von Henri Hubert & Marcel Mauss, Herfried ...

  2. CHARACTER AND FORMS OF INDUSTRIAL GOODS DISTRIBUTION ON DON AND THE NORTH CAUCASUS IN THE GREAT PATRIOTIC WAR DAYS

    Directory of Open Access Journals (Sweden)

    E. V. PANARINA

    2016-01-01

    Full Text Available This article is devoted to realization of a state policy of distribution of industrial goods on Don and the North Caucasus in days of the Great Patriotic War. The principles of use of a rationing system of distribution of essentials, her differentiated character, preferential categories of consumers providing allocation are considered. Concrete examples of application of coupons at acquisition of clothes, footwear and other goods are given. Conditions of execution of this order by local bodies of Don and the North Caucasus are analyzed; the attention to limitation of the allocated manufactured goods from the centralized funds is paid. Much attention is paid to the measures for research of additional sources of providing the population of Don and the North Caucasus taken from local bodies by essentials. The attention to especially difficult situation with providing the population of the region, owing to occupation consequences is paid. Data on restoration and development of the local industry, cooperative artels, increase in release of goods, necessary for the population, are provided. The role of the trade-union organizations in supply of the most needing workers with clothes, footwear and other manufactured goods reveals. The assessment is given to results of collaboration of local bodies and public organizations in providing the population with manufactured goods, the remaining difficult situation in questions of providing inhabitants of Don and the North Caucasus by essentials throughout the entire period of the Great Patriotic War is noted.

  3. PERKEMBANGAN LATISIFER PADA KULTUR KALUS CATHARANTHUS ROSEUS (L) G. DON YANG DIINDUKSI DENGAN KOMBINASI ZAT PENGATUR TUMBUH KINETIN + NAA

    OpenAIRE

    NI NYOMAN DARSINI

    2011-01-01

    The development of laticifer on callus culture of Catharanthus roseus (L) G Don in Zenk medium supplemented with combination of plant growth regulator kinetin + NAA was studied. The explants were taken from the second folium from shoot apex. Development of laticifer was observed using descriptive analysis method for callus anatomy and percentage of laticifer was observed during 4–14 weeks of callus development. The percentage of laticifer was determined by counting the average number of the l...

  4. El arzobispado de Don Gonzalo de Aguilar en Santiago de Compostela (1348-1351

    Directory of Open Access Journals (Sweden)

    Sánchez Sánchez, Xosé M.

    2010-06-01

    Full Text Available In the middle of the 14th century the prelature of Santiago de Compostela is occupied by Don Gonzalo de Aguilar, archbishop transferred from Sigüenza and whose pontificate is extended between 1348 and 1351, when he is transferred to Toledo. He is a figure that remained until now in the complete darkness of the history of the Compostellan church, disappearing in fact, in many cases, of the historical considerations, that they avoided him or simply they did not have noticed of his figure, extending the bordering prelatures (Peter V and Gómez Manrique until eliminate his one. It responds to the completely absence of sources and mentions in the Compostellan archives; in order to palliate this fact, we resorted to the bibliography of other seats and, specially, to the sources of the Vatican Secret Archive, with whose aid we can form one first, new and relatively complete vision of its reign in Santiago.

    A mediados del siglo XIV ocupa el arzobispado de Santiago de Compostela Don Gonzalo de Aguilar, prelado trasladado desde Sigüenza y cuyo pontificado se extiende entre los años 1348 y 1351, momento en que es promovido a Toledo. Se trata de una figura que permanecía hasta ahora en la completa penumbra de la historia de la iglesia compostelana, hasta el punto de haber desaparecido en muchos casos de las consideraciones históricas, que obviaban o simplemente desconocían su figura, extendiendo las prelaturas limítrofes, las de Pedro V y Gómez Manrique, hasta eliminar la suya. Ello responde a la completa falta de fuentes y menciones existente en los archivos compostelanos. Para paliar este hecho, recurrimos tanto a la bibliografía de otras sedes como, especialmente, a las fuentes del Archivo Secreto Vaticano, con cuya ayuda podemos configurar una primera, novedosa y relativamente completa visión de su gobierno en Santiago.

  5. Stokes IQUV magnetic Doppler imaging of Ap stars - I. ESPaDOnS and NARVAL observations

    Science.gov (United States)

    Silvester, J.; Wade, G. A.; Kochukhov, O.; Bagnulo, S.; Folsom, C. P.; Hanes, D.

    2012-10-01

    In this paper we describe and evaluate new spectral line polarization observations obtained with the goal of mapping the surfaces of magnetic Ap stars in great detail. One hundred complete or partial Stokes IQUV sequences, corresponding to 297 individual polarized spectra, have been obtained for seven bright Ap stars using the Échelle SpectroPolarimetric Device for the Observation of Stars (ESPaDOnS) and NARVAL high-resolution spectropolarimeters. The targets span a range of masses from approximately 1.8 to 3.4 M⊙, a range of rotation periods from 2.56 to 6.80 d and a range of maximum longitudinal magnetic field strengths from 0.3 to over 4 kG. For three of the seven stars, we have obtained dense phase coverage sampling the entire rotational cycle. These data sets are suitable for immediate magnetic and chemical abundance surface mapping using magnetic Doppler imaging. For the remaining four stars, partial phase coverage has been obtained, and additional observations will be required in order to map the surfaces of these stars. The median signal-to-noise ratio of the reduced observations is over 700 per 1.8 km s-1 pixel. Spectra of all stars show Stokes V Zeeman signatures in essentially all individual lines, and most stars show clear Stokes QU signatures in many individual spectral lines. The observations provide a vastly improved data set compared to previous generations of observations in terms of signal-to-noise ratio, resolving power and measurement uncertainties. Measurement of the longitudinal magnetic field demonstrates that the data are internally consistent within computed uncertainties typically at the 50-100σ level. Data are also shown to be in excellent agreement with published observations and in qualitative agreement with the predictions of published surface structure models. In addition to providing the foundation for the next generation of surface maps of Ap stars, this study establishes the performance and stability of the ESPaDOnS and NARVAL

  6. Don Delillo’s Point Omega; Ecstasy and Inertia in a Hyperreal World: A Baudrillardian Reading

    Directory of Open Access Journals (Sweden)

    Faeze Yegane

    2015-07-01

    Full Text Available This paper aims to present a Baudrillardian reading of Don Delillo’s Point Omega in the framework of Baudrillard’s definition of the contemporary world as ‘hyperreal’ and also his twin concepts of ‘ecstasy and inertia’. According to Baudrillard, the contemporary time is the hyperreal era in which subjects do not have access to ‘real’ primarily because they are supplied with the ‘simulations’ first and then with the ‘real’ entity and probably never confronted with the ‘real’ itself through media, advertisements, and virtual world. Thus, the perception they have from incidents, objects, places and even other people is ‘hyperreal’; edited, censored, beautified and exaggerated versions of reality; more real than real. In this study Point Omega will be examined as Delillo’s ‘hyperreal’ version of Alfred Hitchcock’s Psycho since the movie is screened in the course of the novel and despite the similarities between the novel and the movie they end contrastingly. Symbolically ‘real’ is not found in the novel due to ‘Mobius spiraling negativity’ which is one of the features of Baudrillard’s definition of ‘hyperreal’ age. Baudrillard believes in the triumph of objects over subjects. While the object’s world is perpetually cultivating frenziedly, objects and technologies begin to dominate the stupefied subjects consequently he states when the objects are moving toward their ‘ecstasy’, the subjects are stricken in ‘inertia’. This supremacy of objects and technologies will be displayed in Point Omega regarding Richard Elster’s inert behavior and reaching the ‘omega point’ that Teilhard de Chardin envisions for human race is rendered impossible due to Elster’s destiny in the framework of Baudrillard’s concept of evolution.  Keywords: Ecstasy, Inertia, Hyperreality, Baudrillard, Don Delillo, Point Omega, Omega Point, Teilhard de Chardin, Alfred Hitchcock.    

  7. Stochastic models of cell motility

    DEFF Research Database (Denmark)

    Gradinaru, Cristian

    2012-01-01

    interdisciplinary field focusing on the study of biological processes at the nanoscale level, with a range of technological applications in medicine and biological research, has emerged. The work presented in this thesis is at the interface of cell biology, image processing, and stochastic modeling. The stochastic...... coefficient. By adding a persistence component to simple random motion I introduce the standard Ornstein-Uhlenbeck process. I build on this commonly used cell motility model to address the challenges of working with real-life data: positional (centroid coordinate measuring) error and time discretization (due...... generalizing the Orstein-Uhlenbeck process to study cell motility on anisotropic substrates. I apply the general model to analyze cell motility on a series of anisotropic substrates and discuss the implications of our observations. This work is potentially useful to cell biologists by addressing their need for...

  8. Computational methods for multi-omic models of cell metabolism and their importance for theoretical computer science

    OpenAIRE

    Angione, Claudio

    2015-01-01

    To paraphrase Stan Ulam, a Polish mathematician who became a leading figure in the Manhattan Project, in this dissertation I focus not only on how computer science can help biologists, but also on how biology can inspire computer scientists. On one hand, computer science provides powerful abstraction tools for metabolic networks. Cell metabolism is the set of chemical reactions taking place in a cell, with the aim of maintaining the living state of the cell. Due to the intrinsic complexity of...

  9. Conrad Hal Waddington: the last Renaissance biologist?

    Science.gov (United States)

    Slack, Jonathan M W

    2002-11-01

    Conrad Hal Waddington was a leading embryologist and geneticist from the 1930s to the 1950s. He is remembered mainly for his concepts of the 'epigenetic landscape' and 'genetic assimilation'. This article reviews his life and work, and enquires to what extent his ideas are relevant tools for understanding the biological problems of today. PMID:12415319

  10. Toward Better Physics Labs for Future Biologists

    CERN Document Server

    Moore, K; Losert, W

    2013-01-01

    We have developed a set of laboratories and hands on activities to accompany a new two-semester interdisciplinary physics course that has been successfully developed and tested in two small test classes of students at the University of Maryland, College Park (UMD) in 2012-2013. We have designed the laboratories to be taken accompanying a reformed course in the student's second year, with calculus, biology, and chemistry as prerequisites. This permits the laboratories to include significant content on physics relevant to cellular scales, from chemical interactions to random motion and charge screening in fluids. We also introduce the students to research-grade equipment and modern physics analysis tools in contexts relevant to biology, while maintaining the pedagogically valuable open-ended laboratory structure of reformed laboratories. Preliminary student results from these two small test classes are discussed.

  11. Hilda Cid: physicist, crystallographer, structural biologist

    Science.gov (United States)

    Gutiérrez, Gonzalo

    2016-05-01

    A brief review of Dr. Hilda Cid, Chilean scientist who excelled in the field of crystallography, is presented. Dr. Cid was born in 1933 in Talcahuano, where she completed her primary and secondary education, graduating as a Teacher of mathematics and physics from the University of Chile in 1958. She continued graduate studies at MIT, USA, where obtained the PhD in 1964. Upon her return to Chile, she joined the Austral University in Valdivia, where she dedicated to characterize, by X-ray, biological material. During that time, she was actively involved in the social changes taking place in the university and in the country. Thus, following the bloody coup of 1973, she and her family were persecuted and forced to go into exile to Sweden. In the decade of the 1980 she returns to Chile, and settles at University of Concepción, where she teaches, conducts research and organizes a number of latino-american courses and symposiums. Knowing that Hilda Cid formed a real school, where her students -now distinguished professionals and researchers- cast their teachings through Chile and other countries, we ended the article with a reflection about why Hilda Cid, despite its obvious scientific achievements, remains ignored by most of the scientific establishment in Chile.

  12. Intelligent systems for the molecular biologist

    Energy Technology Data Exchange (ETDEWEB)

    Brutlag, D.L.

    1995-12-31

    This tutorial was one of eight tutorials selected to be presented at the Third International Conference on Intelligent Systems for Molecular Biology which was held in the United Kingdom from July 16 to 19, 1995. In this paper, one objective is to identify properties of DNA sequences that determine their function, by computer-aided statistical analysis and to accurately predict its function, given a new sequence. A related problem is to predict protein structure and function from the sequence.

  13. Genetics of asthma: a molecular biologist perspective

    Directory of Open Access Journals (Sweden)

    Ghosh Balaram

    2009-05-01

    Full Text Available Abstract Asthma belongs to the category of classical allergic diseases which generally arise due to IgE mediated hypersensitivity to environmental triggers. Since its prevalence is very high in developed or urbanized societies it is also referred to as "disease of civilizations". Due to its increased prevalence among related individuals, it was understood quite long back that it is a genetic disorder. Well designed epidemiological studies reinforced these views. The advent of modern biological technology saw further refinements in our understanding of genetics of asthma and led to the realization that asthma is not a disorder with simple Mendelian mode of inheritance but a multifactorial disorder of the airways brought about by complex interaction between genetic and environmental factors. Current asthma research has witnessed evidences that are compelling researchers to redefine asthma altogether. Although no consensus exists among workers regarding its definition, it seems obvious that several pathologies, all affecting the airways, have been clubbed into one common category called asthma. Needless to say, genetic studies have led from the front in bringing about these transformations. Genomics, molecular biology, immunology and other interrelated disciplines have unearthed data that has changed the way we think about asthma now. In this review, we center our discussions on genetic basis of asthma; the molecular mechanisms involved in its pathogenesis. Taking cue from the existing data we would briefly ponder over the future directions that should improve our understanding of asthma pathogenesis.

  14. Nuclear power plant transient diagnostics using artificial neural networks that allow ''don't-know'' classifications

    International Nuclear Information System (INIS)

    A nuclear power plant's (NPP's) status is usually monitored by a human operator. Any classifier system used to enhance the operator's capability to diagnose a safety-critical system like an NPP should classify a novel transient as ''don't-know'' if it is not contained within its accumulated knowledge base. In particular, the classifier needs some kind of proximity measure between the new data and its training set. Artificial neural networks have been proposed as NPP classifiers, the most popular ones being the multilayered perceptron (MLP) type. However, MLPs do not have a proximity measure, while learning vector quantization, probabilistic neural networks (PNNs), and some others do. This proximity measure may also serve as an explanation to the classifier's decision in the way that case-based-reasoning expert systems do. The capability of a PNN network as a classifier is demonstrated using simulator data for the three-loop 436-MW(electric) Westinghouse San Onofre unit 1 pressurized water reactor. A transient's classification history is used in an ''evidence accumulation'' technique to enhance a classifier's accuracy as well as its consistency

  15. E-mail netiquette for the medical practice employee: 50 do's and don'ts.

    Science.gov (United States)

    Hills, Laura

    2011-01-01

    E-mail has at one and the same time become a primary communication tool for many medical practice employees and a source of untold frustration, misunderstandings, and stress. This article provides a total of 50 rules (25 do's and 25 don'ts) for e-mail netiquette to help medical practice employees use e-mail more effectively. It offers suggestions about the optimal format of e-mails, tips for writing concise e-mail texts, and guidance about the most pressing e-mail privacy and confidentiality concerns. Medical practice employees can use the practical tips presented in this article to help them save time and use their medical practices' e-mail system more productively and effectively. This article also offers suggestions for creating and using a medical practice e-mail disclaimer statement. It describes the six legal threats a disclaimer statement can prevent and the typical content for such disclaimers. Finally, this article includes guidance about creating and implementing a medical practice e-mail policy and provides an outline of typical e-mail policy content. PMID:22111286

  16. Stokes IQUV Magnetic Doppler Imaging of Ap stars I. ESPaDOnS and NARVAL Observations

    CERN Document Server

    Silvester, J; Kochukhov, O; Bagnulo, S; Folsom, C P; Hanes, D

    2012-01-01

    In this paper we describe and evaluate new spectral line polarisation observations obtained with the goal of mapping the surfaces of magnetic Ap stars in great detail. One hundred complete or partial Stokes IQUV sequences, corresponding to 297 individual polarised spectra, have been obtained for 7 bright Ap stars using the ESPaDOnS and NARVAL spectropolarimeters. The targets span a range of mass from approximately 1.8 to 3.4 solar mass, a range of rotation period from 2.56 to 6.80 days, and a range of maximum longitudinal magnetic field strength from 0.3 to over 4 kG. For 3 of the 7 stars, we have obtained dense phase coverage sampling the entire rotational cycle. These datasets are suitable for immediate magnetic and chemical abundance surface mapping using Magnetic Doppler Imaging (MDI). For the 4 remaining stars, partial phase coverage has been obtained, and additional observations will be required in order to map the surfaces of these stars. The median signal-to-noise ratio of the reduced observations is ...

  17. Inversion of stellar fundamental parameters from ESPaDOnS and Narval high-resolution spectra

    Science.gov (United States)

    Paletou, F.; Böhm, T.; Watson, V.; Trouilhet, J.-F.

    2015-01-01

    The general context of this study is the inversion of stellar fundamental parameters from high-resolution Echelle spectra. We aim at developing a fast and reliable tool for the post-processing of spectra produced by ESPaDOnS and Narval spectropolarimeters. Our inversion tool relies on principal component analysis. It allows reducing dimensionality and defining a specific metric for the search of nearest neighbours between an observed spectrum and a set of observed spectra taken from the Elodie stellar library. Effective temperature, surface gravity, total metallicity, and projected rotational velocity are derived. Various tests presented in this study that were based solely on information coming from a spectral band centred on the Mg i b-triplet and had spectra from FGK stars are very promising. Based on observations obtained at the Télescope Bernard Lyot (TBL, Pic du Midi, France), which is operated by the Observatoire Midi-Pyrénées, Université de Toulouse, Centre National de la Recherche Scientifique (France) and the Canada-France-Hawaii Telescope (CFHT) which is operated by the National Research Council of Canada, CNRS/INSU and the University of Hawaii (USA).

  18. 60 years of CERN at UNESCO | Don't miss the webcast! | 1 July

    CERN Multimedia

    2014-01-01

    Don't miss the live webcast of the commemoration ceremony, marking the signature of the CERN Convention. The ceremony will be held at the UNESCO Headquarters in Paris on 1 July, from 10 a.m.   To watch the webcast, please visit: http://webcast.web.cern.ch/webcast/ from 10 a.m. Programme 10 a.m. - 11 a.m.: Opening of Ceremony by Maciej Nalecz, Director of the Division of Science Policy and Capacity Building, UNESCO   Address by Irina Bokova, Director-General of UNESCO   Address by Rolf Heuer, Director-General of CERN   Video clip “CERN: 60 years of science for peace”   Address by Agnieszka Zalewska, President of the CERN Council   Hommage à François de Rose, un des pères fondateurs du CERN, par sa fille Laurence Rousselot   CERN as seen by a young phycisist, Claire Lee (ATLAS Collaboration) 11 a.m. - 12 p.m.: Roundtable discussion on “Science for...

  19. Shattering Man’s Fundamental Assumptions in Don DeLillo’s Falling Man

    Directory of Open Access Journals (Sweden)

    Hazim Adnan Hashim

    2016-09-01

    Full Text Available The present study addresses effects of traumatic events such as the September 11 attacks on victims’ fundamental assumptions. These beliefs or assumptions provide individuals with expectations about the world and their sense of self-worth. Thus, they ground people’s sense of security, stability, and orientation. The September 11 terrorist attacks in the U.S.A. were very tragic for Americans because this fundamentally changed their understandings about many aspects in life. The attacks led many individuals to build new kind of beliefs and assumptions about themselves and the world. Many writers have written about the human ordeals that followed this incident. Don DeLillo’s Falling Man reflects the traumatic repercussions of this disaster on Americans’ fundamental assumptions. The objective of this study is to examine the novel from the traumatic perspective that has afflicted the victims’ fundamental understandings of the world and the self. Individuals’ fundamental understandings could be changed or modified due to exposure to certain types of events like war, terrorism, political violence or even the sense of alienation. The Assumptive World theory of Ronnie Janoff-Bulman will be used as a framework to study the traumatic experience of the characters in Falling Man. The significance of the study lies in providing a new perception to the field of trauma that can help trauma victims to adopt alternative assumptions or reshape their previous ones to heal from traumatic effects.Keywords: benevolence, meaningfulness, self-worthiness, trauma, post-traumatic stress disorder

  20. Proteomic profiling of proteins associated with the rejuvenation of Sequoia sempervirens (D. Don Endl

    Directory of Open Access Journals (Sweden)

    Chen Yu-Ting

    2010-12-01

    Full Text Available Abstract Background Restoration of rooting competence is important for rejuvenation in Sequoia sempervirens (D. Don Endl and is achieved by repeatedly grafting Sequoia shoots after 16 and 30 years of cultivation in vitro. Results Mass spectrometry-based proteomic analysis revealed three proteins that differentially accumulated in different rejuvenation stages, including oxygen-evolving enhancer protein 2 (OEE2, glycine-rich RNA-binding protein (RNP, and a thaumatin-like protein. OEE2 was found to be phosphorylated and a phosphopeptide (YEDNFDGNSNVSVMVpTPpTDK was identified. Specifically, the protein levels of OEE2 increased as a result of grafting and displayed a higher abundance in plants during the juvenile and rejuvenated stages. Additionally, SsOEE2 displayed the highest expression levels in Sequoia shoots during the juvenile stage and less expression during the adult stage. The expression levels also steadily increased during grafting. Conclusion Our results indicate a positive correlation between the gene and protein expression patterns of SsOEE2 and the rejuvenation process, suggesting that this gene is involved in the rejuvenation of Sequoia sempervirens.

  1. "Don't know" answers concerning somatic disease status should not be regarded as "no" responses

    Directory of Open Access Journals (Sweden)

    Baumeister, Harald

    2008-06-01

    Full Text Available Background: With regard to patients' self-reported somatic diseases some researchers transformed “don't know (DK” responses into “no” responses. The present study examines the appropriateness of this procedure. Methods: Analyses were based on the nationally representative German National Health Interview and Examination Survey (GHS, which assessed both self-reported diseases and physician-diagnosed diseases (N = 7124. Prevalence rates of persons’ DK responses and the corresponding prevalences of physicians’ diagnoses were calculated for persons with hypertension, coronary heart disease (CHD, heart failure, asthma, chronic bronchitis, thyroid disease, diabetes, cancer, gout, arthrosis, arthritis and osteoporosis. Correlates of physicians' diagnosed diseases of DK cases are reported. Results: Between 1.6% and 9.8% of the participants responded with DK to the question of whether they have the disease. In 3.7% to 29.5% of DK cases, the physicians did regard the respective disease as being present. With regard to persons who responded with DK, the probability of a physicians' diagnosis was increased in the case of increased age and a higher number of somatic comorbidities. Conclusion: The procedure of transforming DK responses into “no” answers does not appear to be recommendable.

  2. Don Draper Thinks Your Ad Is Cliché: Fairy Tale Iconography in TV Commercials

    Directory of Open Access Journals (Sweden)

    Preston Wittwer

    2016-05-01

    Full Text Available When examining the history of fairy tale iconography in advertising, folklore scholar Donald Haase’s fairy tale encyclopedia compared the Pied Piper of Hamelin to a symbol of advertising who could “play his pipe ever so sweetly and the consumers following him without resisting his charming and manipulative music.” In contrast, a 2012 episode of Mad Men, advertising luminary Don Draper shoots down a shoe commercial pitch featuring Cinderella, calling the idea “cliché”. The temptation for advertisers to rely on fairy tale figures and iconography continues today and many ignore Don’s aversion for cliché because it still gets the job done. However, there are some ads featuring fairy tales which avoid cliché and are truly innovative for their time. I’ll examine how, and for whom, these fairy tale figures have been adapted decade by decade in order to examine popular culture’s commercialized and hypnotic relationship with fairy tales in the most direct format available: television commercials.

  3. Effect of Salinity Stress on Seed Germination Catharanthus roseus Don. Cvs. Rosea and Alba

    Directory of Open Access Journals (Sweden)

    Zahra Rezaee

    2012-03-01

    Full Text Available This experiment was carried out aiming to determine the Catharanthus roseus Don resistance against salinity, due to the increasing salinity of soil, and the importance of this plant as an ornamental flower, as well as the little information available on its tolerance against salinity during the germination period. In order to an experiment was conducted in randomized completely design factorial. Sodium chloride was used for induce salinity stress. These factors include cultivar and salinity levels with Electrical Conductivity (EC of 1, 2, 4, 6, 8, and 10 dS/m. The results of variance analysis showed that salinity effect at level 1% on the germination percentage, germination rate, radicle and plumule length, seedling wet and dry weight and ratio of plumule length to radicle length were significant and they were reduced. While the cultivar and interaction of cultivar and salinity treatments were non significant. According to the results, response of two cultivars to the salinity was the same and there was no difference between them.

  4. Synthesis of silver nanoparticles using leaves of Catharanthus roseus Linn. G. Don and their antiplasmodial activities

    Science.gov (United States)

    Ponarulselvam, S; Panneerselvam, C; Murugan, K; Aarthi, N; Kalimuthu, K; Thangamani, S

    2012-01-01

    Objective To develop a novel approach for the green synthesis of silver nanoparticles using aqueous leaves extracts of Catharanthus roseus (C. roseus) Linn. G. Don which has been proven active against malaria parasite Plasmodium falciparum (P. falciparum). Methods Characterizations were determined by using ultraviolet-visible (UV-Vis) spectrophotometry, scanning electron microscopy (SEM), energy dispersive X-ray and X-ray diffraction. Results SEM showed the formation of silver nanoparticles with an average size of 35–55 nm. X-ray diffraction analysis showed that the particles were crystalline in nature with face centred cubic structure of the bulk silver with the broad peaks at 32.4, 46.4 and 28.0. Conclusions It can be concluded that the leaves of C. roseus can be good source for synthesis of silver nanoparticle which shows antiplasmodial activity against P. falciparum. The important outcome of the study will be the development of value added products from medicinal plants C. roseus for biomedical and nanotechnology based industries. PMID:23569974

  5. Synthesis of silver nanoparticles using leaves of Catharanthus roseus Linn. G. Don and their antiplasmodial activities

    Institute of Scientific and Technical Information of China (English)

    Ponarulselvam S; Panneerselvam C; Murugan K; Aarthi N; Kalimuthu K; Thangamani S

    2012-01-01

    Objective: To develop a novel approach for the green synthesis of silver nanoparticles using aqueous leaves extracts of Catharanthus roseus (C. roseus) Linn. G. Don which has been proven active against malaria parasite Plasmodium falciparum (P. falciparum). Methods:Characterizations were determined by using ultraviolet-visible (UV-Vis) spectrophotometry, scanning electron microscopy (SEM), energy dispersive X-ray and X-ray diffraction. Results:SEM showed the formation of silver nanoparticles with an average size of 35-55 nm. X-ray diffraction analysis showed that the particles were crystalline in nature with face centred cubic structure of the bulk silver with the broad peaks at 32.4, 46.4 and 28.0. Conclusions: It can be concluded that the leaves of C. roseus can be good source for synthesis of silver nanoparticle which shows antiplasmodial activity against P. falciparum. The important outcome of the study will be the development of value added products from medicinal plants C. roseus for biomedical and nanotechnology based industries.

  6. Analysis of Multiple Partially Ordered Responses to Belief Items with Don't Know Option.

    Science.gov (United States)

    Ip, Edward H; Chen, Shyh-Huei; Quandt, Sara A

    2016-06-01

    Understanding beliefs, values, and preferences of patients is a tenet of contemporary health sciences. This application was motivated by the analysis of multiple partially ordered set (poset) responses from an inventory on layman beliefs about diabetes. The partially ordered set arises because of two features in the data-first, the response options contain a Don't Know (DK) option, and second, there were two consecutive occasions of measurement. As predicted by the common sense model of illness, beliefs about diabetes were not necessarily stable across the two measurement occasions. Instead of analyzing the two occasions separately, we studied the joint responses across the occasions as a poset response. Few analytic methods exist for data structures other than ordered or nominal categories. Poset responses are routinely collapsed and then analyzed as either rank ordered or nominal data, leading to the loss of nuanced information that might be present within poset categories. In this paper we developed a general class of item response models for analyzing the poset data collected from the Common Sense Model of Diabetes Inventory. The inferential object of interest is the latent trait that indicates congruence of belief with the biomedical model. To apply an item response model to the poset diabetes inventory, we proved that a simple coding algorithm circumvents the requirement of writing new codes such that standard IRT software could be directly used for the purpose of item estimation and individual scoring. Simulation experiments were used to examine parameter recovery for the proposed poset model. PMID:25479822

  7. The potential for detecting `life as we don't know it' by fractal complexity analysis

    Science.gov (United States)

    Azua-Bustos, Armando; Vega-Martínez, Cristian

    2013-10-01

    Finding life in the Universe entirely different to the one evolved on Earth is probable. This is a significant constraint for life-detecting instruments that were sent and may be sent elsewhere in the solar system, as how could we detect life as `we don't know it'? How could we detect something when we have no prior knowledge of its composition or how it looks like? Here we argue that disregarding the type of lifeform that could be envisioned, all must share in common the attribute of being entities that decrease their internal entropy at the expense of free energy obtained from its surroundings. As entropy quantifies the degree of disorder in a system, any envisioned lifeform must have a higher degree of order than its supporting environment. Here, we show that by using fractal mathematics analysis alone, one can readily quantify the degree of entropy difference (and thus, their structural complexity) of living processes (lichen growths and plant growing patterns in this case) as distinct entities separate from its similar abiotic surroundings. This approach may allow possible detection of unknown forms of life based on nothing more than entropy differentials of complementary datasets. Future explorations in the solar system, like Mars or Titan, may incorporate this concept in their mission planning in order to detect potential endemic lifeforms.

  8. Monoamine oxidase B and free radical scavenging activities of natural flavonoids in Melastoma candidum D. Don.

    Science.gov (United States)

    Lee, M H; Lin, R D; Shen, L Y; Yang, L L; Yen, K Y; Hou, W C

    2001-11-01

    Monoamine oxidase type B (MAO-B) activity and free radicals are elevated in certain neurological diseases. Four natural flavonoids, quercitrin, isoquercitrin, rutin, and quercetin, were isolated for the first time from the leaves of Melastoma candidum D. Don. They exhibited an inhibitory effect on MAO-B. These potent flavonoids were purified using bioassay-guided fractionation and were separated by Diaion, Sephadex LH-20, and MCI CHP20P columns. The IC(50) values of the four potent flavonoids, quercitrin, isoquercitrin, rutin, and quercetin on monoamine oxidase were 19.06, 11.64, 3.89, and 10.89 microM and enzyme kinetics analysis revealed apparent inhibition constants (K(i)) of 21.01, 2.72, 1.83, and 7.95 microM, respectively, on the substrate, benzylamine. The four potent compounds also exhibited hydroxyl radical scavenging activity as determined using a spin trapping electron spin resonance method. This suggests that the four flavonoids from M. candidum possess both MAO-B inhibitory and free radical scavenging activities. These important properties may be used for preventing some neurodegenerative diseases in the future. PMID:11714358

  9. CELL-SURFACE BINDING OF DEOXYNIVALENOL TO Lactobacillus paracasei subsp. tolerans ISOLATED FROM SOURDOUGH STARTER CULTURE

    Directory of Open Access Journals (Sweden)

    Yousef I. Hassan

    2013-04-01

    Full Text Available Deoxynivalenol (DON and fumonisin B1 (FB1 are two contaminant-mycotoxins frequently found in food commodities produced under poor conditions. Several methods have been suggested for the detoxification of such mycotoxins. Among the proposed methods, biological detoxification seems to be the most promising and cost-efficient. This study explores the capability of one strain of lactic acid bacteria, identified as Lactobacillus paracasei subsp. tolerans, to bind both DON and FB1 in liquid cultures. Here we report the ability of heat-inactivated cells to significantly reduce concentrations of DON in liquid cultures. Further mechanistic investigation showed that the detoxification process is a result of the physical binding of such mycotoxins to the cell wall of this bacterium.

  10. Immunomodulatory effects of individual and combined mycotoxins in the THP-1 cell line.

    Science.gov (United States)

    Solhaug, A; Karlsøen, L M; Holme, J A; Kristoffersen, A B; Eriksen, G S

    2016-10-01

    Mycotoxins commonly contaminate food and may pose a risk for disease in humans and animals. As they frequently co-occur, mixed exposures often take place. Monocyte function, including differentiation into active macrophages, is a central part of the immune response. Here we studied effects of naturally co-occurring mycotoxins in grain on monocyte function, and effects of individual and combined exposure on the differentiation process from monocytes into macrophages. The THP-1 cell line was used as a model system. The mycotoxins 2-amino-14,16-dimethyloctadecan-3-ol (AOD), alternariol (AOH), enniatin B (ENNB), deoxynivalenol (DON), sterigmatocystin (ST) and zearalenone (ZEA) differently affected cell viability in THP-1 monocytes, with DON as the most potent. AOH, ZEA and DON inhibited differentiation from monocytes into macrophages. Using this differentiation model, combined exposure of AOH, ZEA and DON were mainly found to be additive. However, the combination AOH+ZEA had somewhat synergistic effect at lower concentrations. Furthermore, alterations in macrophage functionality were found, as single exposure of AOH and ZEA inhibited lipopolysaccharide (LPS) induced TNF-α secretion, while DON increased this response. Overall, the mycotoxins affected monocyte viability and differentiation into macrophages differently. Combined exposures affected the differentiation process mainly additively. PMID:27453131

  11. Advances in stem cell research

    Institute of Scientific and Technical Information of China (English)

    2001-01-01

    @@In 1998, biologists Thomson and Gearhart successfully derived stem cells from human embryos. One year later, several researchers discovered that adult stem cells still retain the ability to be differentiated into unrelated types of cells. Advances in stem cell research open a promising direction for applied medical science. Moreover, it may also force scientists to reconsider the fundamental theory about how cells grow up. Stem cell research was considered by Science as the top of the ten breakthroughs of science of the year[1]. This paper gives a survey of recent advances in stem cell research. 1 Overview In the 1980s, embryonic stem cell and/or embryonic germ cell line (ES cell line, EG cell line) of multifarious mammalian animals, especially those of non-human pri-mates, had been established. In 1998, Thomson and Shamblott obtained ES, EG cell lines from human blasto-cysts and gonad ridges of early human embryos, respec-tively. Their research brought up an ethical debate about whether human embryos can be used as experimental materials. It was not appeased until 1999 when research-ers discovered that stem cells from adults still retain the ability to become different kinds of tissue cells. For in-stance, brain cells can become blood cells[2], and cells from bone marrow can become cells in liver. Scientists believe, for a long time, that cells can only be developed from early pluripotent embryo cells; the differentiation potential of stem cells from mature tissues is restricted to only one of the cell types of the tissue where stem cells are obtained. Recent stem cell researches, however, sub-verted the traditional view of stem cells. These discoveries made scientists speed ahead with the work on adult stem cells, hoping to discover whether their promise will rival that of ES cells.

  12. DON QUIJOTE COMO NOVELA MODERNA Y LA CONJUNCIÓN DE GÉNEROS ALTOS Y BAJOS Don Quixote as a modern novel and the mixture of high and low genres

    Directory of Open Access Journals (Sweden)

    Manuel Jofré

    2006-07-01

    Full Text Available Partiendo de los aportes de la crítica cervantina, se indagan las nuevas razones para considerar El Quijote como primera novela moderna, a partir de las ideas de Mijail Bajtín. La gran obra de Cervantes emergería no sólo como superación de la línea monológica sino como intersección entre la línea monológica y la línea dialógica de la literatura universal. Esto se manifestaría en la conjunción de géneros altos con géneros bajos, primero, y segundo, en la interfertilización de los discursos sublimes y caballerescos de don Quijote con los discursos campesinos y populares de Sancho. Este verdadero dialogismo se basaría en la intertextualidad de la novela de Cervantes con el diálogo socrático y con la sátira menipea de la antigüedad.Within the context of the Cervantine criticism, new reasons are discussed in this article to consider Don Quixote as the first modern novel, from the point of view of Mijail Bakhtin writings. Cervantes's great work would not only overcome the monologic line, but more precisely it would constitute an intersection between the monologic and dialogic lines of the Universal Literature. This would be evident in the mixture of higher and lower literary genre and in the opposition between Don Quixote´s sublime and knight-like speeches and Sancho´s folk and popular speeches. This truly dialogic relationship would be based upon the intertextuality of Cervantes’s work with the Socratic dialog and the Mineppean satire of antiquity.

  13. Wound healing properties and kill kinetics of Clerodendron splendens G. Don, a Ghanaian wound healing plant

    Directory of Open Access Journals (Sweden)

    Stephen Y Gbedema

    2010-01-01

    Full Text Available As part of our general objective of investigating indigenous plants used in wound healing in Ghana, we hereby report our findings from some in vitro and in vivo studies related to wound healing activities of Clerodendron splendens G. Don (Verbanaceae. Methanolic extract of the aerial parts of the plant was tested for antimicrobial activity against Gram positive bacteria (Bacillus subtilis, Staphylococcus aureus, Streptococcus faecalis, Micrococcus flavus, as well as resistant strains of Staph. aureus SA1199B, RN4220 and XU212, Gram negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Proteous mirabilis, Klebsiella pneumoniae and Candida albicans using the micro-well dilution method. Survivor-time studies of the microorganisms, radical scavenging activity using 2,2′-diphenylpicrylhydrazyl (DPPH and various in vivo wound healing activity studies were also conducted on the extract. The extract exhibited biostatic action against all the test microorganisms with a Minimum Inhibition Concentration (MIC ranging between 64 and 512 μg/ml and a free radical scavenging property with an IC 50 value of 103.2 μg/ml. The results of the in vivo wound healing tests showed that upon application of C. splendens ointment, there was a reduction in the epithelization period from 26.7 days (control to 13.6 days along with a marked decrease in the scar area from 54.2 mm 2 (control to 25.2 mm 2 . Significant increase in the tensile strength and hydroxyproline content were also observed as compared to the control and was comparable to nitrofurazone. The above results appear to justify the traditional use of C. splendens in wound healing and treatment of skin infections in Ghana.

  14. La sombra del arcediano. El linaje oculto de don Lorenzo Galíndez de Carvajal

    Directory of Open Access Journals (Sweden)

    Baltasar CUART MONER

    2009-11-01

    Full Text Available RESUMEN: D. Lorenzo Galíndez de Carvajal fue un personaje destacado en la Corte de los Reyes Católicos y Carlos V, además de cronista real. Sin embargo, para alcanzar esta posición tuvo que manipular sus orígenes familiares, siendo como era hijo de un arcediano de Coria y de una moza de servicio en una posada. Las manipulaciones de don Lorenzo tuvieron el éxito apetecido, sin embargo, treinta años después de su muerte, las informaciones realizadas sobre un nieto suyo que quería ingresar en el Colegio Mayor de Oviedo, en Salamanca, sacaron a la luz toda la estrategia familiar que tuvo que poner en marcha su ilustre abuelo para alcanzar la posición a la que llegó.ABSTRACT: D. Lorenzo Galíndez de Carvajal was one of the most influential courtiers at the Catholic King's court. He, too, was one of their chroniclers, but he had to forge his familiar origins in order to hold his job. He was an illegitimate son; his father was an archdeacon in Coria and his mother a maidservant. D. Lorenzo Galíndez de Carvajal succeeded in hiding his origins and his descendants became related to nobility by marriage. Howeyer all his grandfather's impostures and the ways followed by aristocracy to manipulate its origins were discovered when one of his grandsons tried to be admitted to the Oviedo College about the middle of the XVIth century.

  15. Characterization of Alkaloid Uptake by Catharanthus roseus (L.) G. Don Protoplasts 1

    Science.gov (United States)

    McCaskill, David G.; Martin, DeAndra L.; Scott, A. Ian

    1988-01-01

    The accumulation of alkaloids by protoplasts of Catharanthus roseus (L.) G. Don var. Little Bright Eye was studied to determine the specificity of uptake and the role of ion trapping in the storage of alkaloids. Accumulation of the indole alkaloids vindoline, ajmalicine, tabersonine, and vinblastine was found to be biphasic, with an initial burst of uptake followed by a slow, prolonged phase of accumulation. The concentration and pH dependence of the initial burst of uptake for vindoline suggested that uptake occurred by simple diffusion. Uptake of nicotine was monophasic, with a half life of 5.2 minutes. The accumulation ratio (Ci/Ce) for nicotine at steady state and for the initial burst of uptake for vindoline and ajmalicine suggested that accumulation was driven by the pH gradient between the vacuole and the external assay medium. The second, sustained phase of uptake of vindoline was sensitive to inhibition by either 20 millimolar NaN3 or 0.5 millimolar Cu2+. In azide-treated protoplasts, the uptake for vindoline conformed to the kinetics of simple diffusion, with a half life of 4 minutes. The second phase of uptake for ajmalicine, although sensitive to inhibition by Cu2+, was insensitive to inhibition by NaN3. The biphasic uptake of the indole alkaloids was not due to any significant metabolism. It is concluded that accumulation and storage of the indole alkaloids is due only partly to ion trapping of the alkaloids by the low pH of the vacuole lumen. In the case of vindoline, there appears to be a specific energy-requiring uptake that is not seen with nicotine (which is not endogenous to Catharanthus). Accumulation of ajmalicine appears to involve both ion trapping and an azide-insensitive component, which may be due to complexation with organic counterions and phenolics. PMID:16666154

  16. Sombreamento de plantas de Catharanthus roseus (L.) G. Don 'Pacifica White' por malhas coloridas: desenvolvimento vegetativo Shading of 'Pacifica White' Catharanthus roseus (L.) G. Don plants with colored nets: vegetative development

    OpenAIRE

    Anderson Adriano Martins Melo; Amauri Alves de Alvarenga

    2009-01-01

    As malhas coloridas têm sido utilizadas para manipular o desenvolvimento vegetativo, melhorando a utilização da radiação solar por plantas ornamentais. Objetivou-se, neste trabalho estudar o efeito da redução de 50% da radiação fotossinteticamente ativa sobre o crescimento vegetativo de plantas de Catharanthus roseus (L.) G. Don, por meio de malhas azul e vermelha e malha preta, em comparação com plantas crescidas na ausência de sombreamento (pleno sol). As plantas foram obtidas a partir de s...

  17. A neural model for transient identification in dynamic processes with 'don't know' response

    Energy Technology Data Exchange (ETDEWEB)

    Mol, Antonio C. de A. E-mail: mol@ien.gov.br; Martinez, Aquilino S. E-mail: aquilino@lmp.ufrj.br; Schirru, Roberto E-mail: schirru@lmp.ufrj.br

    2003-09-01

    This work presents an approach for neural network based transient identification which allows either dynamic identification or a 'don't know' response. The approach uses two 'jump' multilayer neural networks (NN) trained with the backpropagation algorithm. The 'jump' network is used because it is useful to dealing with very complex patterns, which is the case of the space of the state variables during some abnormal events. The first one is responsible for the dynamic identification. This NN uses, as input, a short set (in a moving time window) of recent measurements of each variable avoiding the necessity of using starting events. The other one is used to validate the instantaneous identification (from the first net) through the validation of each variable. This net is responsible for allowing the system to provide a 'don't know' response. In order to validate the method, a Nuclear Power Plant (NPP) transient identification problem comprising 15 postulated accidents, simulated for a pressurized water reactor (PWR), was proposed in the validation process it has been considered noisy data in order to evaluate the method robustness. Obtained results reveal the ability of the method in dealing with both dynamic identification of transients and correct 'don't know' response. Another important point studied in this work is that the system has shown to be independent of a trigger signal which indicates the beginning of the transient, thus making it robust in relation to this limitation.

  18. Stokes IQUV mapping of $\\alpha^2$ CVn & other Ap stars using ESPaDOnS and NARVAL

    OpenAIRE

    Silvester, J.; Kochukhov, O.; Wade, G. A.

    2013-01-01

    New spectral line polarisation observations of 7 bright Ap stars have been obtained with the ESPaDOnS and Narval high resolution spectropolarimeters (Silvester et al. 2012). The aim of this data set is produce a series of surface magnetic field and surface chemistry maps for these Ap stars. We present new magnetic maps for the Ap star $\\alpha^2$ CVn using these new data and the MDI inversion code INVERS10. $\\alpha^2$ CVn is the first Ap star to be observed during two separate epochs using hig...

  19. CHARACTER AND FORMS OF INDUSTRIAL GOODS DISTRIBUTION ON DON AND THE NORTH CAUCASUS IN THE GREAT PATRIOTIC WAR DAYS

    OpenAIRE

    E. V. PANARINA

    2016-01-01

    This article is devoted to realization of a state policy of distribution of industrial goods on Don and the North Caucasus in days of the Great Patriotic War. The principles of use of a rationing system of distribution of essentials, her differentiated character, preferential categories of consumers providing allocation are considered. Concrete examples of application of coupons at acquisition of clothes, footwear and other goods are given. Conditions of execution of this order by local bodie...

  20. Relación interartística en los Cuadernos de Don Rigoberto de Mario Vargas Llosa

    OpenAIRE

    León Restrepo, Ana María

    2012-01-01

    A partir de la lectura de la novela del escritor peruano Mario Vargas Llosa Los Cuadernos de Don Rigoberto, el presente artículo pretende hacer una breve descripción de la relación entre arte y literatura, en específico la intertextualidad pintura y producción verbal, presente en dicha obra -- Este artículo se divide en tres apartados: la contextualización de la novela, sus antecedentes y principales motivos de análisis literario, hasta el desarrollo de la relación mencionada en el párrafo an...

  1. Les dispositifs de sensibilisation au don d'organes : libérer la parole, contraindre l'expression ?

    OpenAIRE

    Masseran, Anne; Chavot, Philippe

    2014-01-01

    Il y a une dizaine d’années, les campagnes officielles et dans une certaine mesure associatives promouvant le don d’organes ont changé de cap. Il s’agissait de transformer le discours, de porter un nouveau regard sur la cible : au début des années 2000 l’individu (isolé) est défini comme un spectateur/donneur, aujourd’hui il est appelé à devenir auteur et acteur de la transplantation d’organes. Les campagnes tendent à susciter un engagement réel et symbolique en incitant les personnes à affir...

  2. Antioxidative Potentials as a Protective Mechanism in Catharanthus roseus (L.) G.Don. Plants under Salinity Stress

    OpenAIRE

    Jaleel, Cheruth Abdul; Gopi, Ragupathi; MANIVANNAN, Paramasivam; Panneerselvam, Rajaram

    2007-01-01

    Antioxidant responses were analysed in Catharanthus roseus (L.) G.Don. under 0, 50 and 100 mM NaCl in order to investigate the plant´s protective mechanisms against long-term salt-induced oxidative stress. The NaCl treatments were repeated in 4 different stages of growth, i.e. 30, 45, 60 and 75 days after sowing (DAS). The plants were uprooted randomly 90 DAS and the non-enzymatic and enzymatic antioxidant potentials were analysed. High salinity caused a decrease in reduced glutathione (GSH) ...

  3. A Glimpse of Stem Cell Research in China

    Institute of Scientific and Technical Information of China (English)

    CHEN Zhi-Guo; ZHANG Y.Alex

    2011-01-01

    2010 has seen rapid progress in stem cell research in China.Not only the major funding agencies had provided extensive funding to stem cell research,the publications by Chinese stem cell biologists also flourished in top-notch scientific journals.In this special review,we highlighted some recent studies that had been published in Science China Life Sciences and Cell Research,two key SCI journals based in China.Not surprisingly,these studies mainly focused on some of the highly pursued stem cell types:embryonic stem cells(ES cells),induced pluripotent stem cells (iPSCs),neural stem cells(NSCs)and mesenchymal stem cells(MSCs).

  4. Colonel Don Benito Machado. A National Guards commandant on the southern frontier of Buenos Aires [1852-1880] El coronel Don Benito Machado. Un comandante de Guardias Nacionales en la frontera sur bonaerense [1852-1880

    Directory of Open Access Journals (Sweden)

    Leonardo Canciani

    2012-01-01

    Full Text Available In this work, we will analyze the National Guard regiments and the role played by their commandants during the process of national organization on the southern frontier of Buenos Aires, from the case study of coronel don José Benito Machado. First of all, our objective will be describe the specific characteristics that adopted the National Guards in the societies of the southern frontier of the province of Buenos Aires. And then, to analyze the mechanisms developed by Benito Machado to build the power bases in the region that enabled the consolidation of its leadershipEn el presente trabajo, analizamos los regimientos de Guardias Nacionales y las funciones que desempeñaron sus comandantes durante el proceso de organización nacional en la frontera sur bonaerense, desde el estudio de caso del coronel don José Benito Machado. En primer lugar, repasamos las características específicas que adoptaron las Guardias Nacionales en las sociedades de la frontera sur de la provincia de Buenos Aires; mientras que, en segundo lugar, analizamos los diversos mecanismos desarrollados por Benito Machado para construir las bases de poder en la región que posibilitaron el afianzamiento de su liderazgo

  5. Stem cell research in China: an intertwinement of international finances, ambition, and bioethics

    OpenAIRE

    Sleeboom, Margaret

    2002-01-01

    Stem cell research promises remedies to widespread diseases ranging from diabetes to paralysis. ‘Stem cell’ is a term used to refer to a range of cells that have the ability to divide into specialized body cells, such as blood cells or new tissue. By studying the processes in which stem cells grow and differentiate, biologists study the causes of many diseases, and hope to use them for therapeutic uses in the repair of damaged tissue and organs for a wide range of currently incurable di...

  6. Re-Gendering the Lbertine; or, The Taming of the Rake: Lucy Vestris as Don Giovanni on the Early Nineteenth-Century London Stage

    OpenAIRE

    Cowgill, Rachel

    1998-01-01

    When Luigi Bassi entered the stage of the Prague National Theatre in 1787 to create the title role of Mozart and Da Ponte's Don Giovanni, he could have drawn inspiration from a rich tradition of theatrical, pantomimic and marionette representations of the legendary Don Juan, to which this new opera was the latest contribution. Previous incarnations had been shaped by the likes of Tirso de Molina, Molière, Shadwell, Purcell and Gluck; yet it is Mozart and Da Ponte's version that has for us bec...

  7. Tradução de Don Juan oder Die Liebe zur Geometrie de Max Frisch e a recepção do autor em Portugal

    OpenAIRE

    Corte-Real, Maria Alexanra Viana de Meneses

    2010-01-01

    O projecto de mestrado Tradução de Don Juan oder Die Liebe zur Geometrie de Max Frisch e a Recepção do Autor em Portugal é composto por duas partes distintas. A primeira parte é dedicada a uma pesquisa da recepção portuguesa do autor. Na segunda parte apresenta-se uma proposta de tradução do drama Don Juan oder Die Liebe zur Geometrie, complementada com uma análise relevante para a tradução que engloba exemplos relevantes.

  8. Los desposorios del gobernador don Francisco de Castellví (1604-1672). Estrategia matrimonial y problemática dotal

    OpenAIRE

    Felipo Orts, Amparo

    2010-01-01

    Este artículo estudia las circunstancias que acompañaron a las sucesivas nupcias del gobernador don Basilio de Castellví con las hijas del regente del Consejo de Aragón don Francisco de Castellví, de los condes de Carlet y de los marqueses de Villasor. Incide en una estrategia matrimonial que participa de la consanguinidad como fortalecimiento de los vínculos en el seno del linaje y de la promoción social mediante el entronque con destacadas casas nobiliarias, así como en el protagonismo adqu...

  9. Comparative Study on the Effect of Different Fertilizers on the Growth and Development of Halenia ellipitica D.Don%不同肥料对椭圆叶花锚生长发育的比较研究

    Institute of Scientific and Technical Information of China (English)

    韩友吉; 李锦萍; 王延花; 张淑玲; 陈桂琛

    2008-01-01

    [Objective] The aim was to study the effect of different treatments on development and growth of Halenia ellipitica D.Don.[Method] Three treatments (urea treatment group, diammonium phosphate treatment group and control group) were set in the experiment with 3 replications to detect plant height,root length, leaf number, branch number, flower number and biomass of Halenia ellipitica D.Don. in all experimental groups.[Result] The leaf number,branch number, flower number and output of crude drug of Halenia ellipitica D.Don. in urea treatment group were more than that of Halenia ellipitica D.Don. in diammonium phosphate treatment group and control group,however,the plant height and root length were not significantly different among all groups.[Conclusion] The urea generated better effect on Halenia ellipitica D.Don. cultivation than that produced by diammonium phosphate treatment.

  10. Why Don't We Already Have an Integrated Framework for the Publication and Preservation of All Data Products?

    Science.gov (United States)

    Accomazzi, A.; Derriere, S.; Biemesderfer, C.; Gray, N.

    2012-09-01

    Astronomy has long had a working network of archives supporting the curation of publications and data. The discipline has already created many of the features which perplex other areas of science: (1) data repositories: (supra)national institutes, dedicated to large projects; a culture of user-contributed data; practical experience of long-term data preservation; (2) dataset identifiers: the community has already piloted experiments, knows what can undermine these efforts, and is participating in the development of next-generation standards; (3) citation of datasets in papers: the community has an innovative and expanding infrastructure for the curation of data and bibliographic resources, and through them a community of authors and editors familiar with such electronic publication efforts; as well, it has experimented with next-generation web standards (e.g. the Semantic Web); (4) publisher buy-in: publishers in this area have been willing to innovate within the constraints of their commercial imperatives. What can possibly be missing? Why don't we have an integrated framework for the publication and preservation of all data products already? Are there technical barriers? We don't believe so. Are there cultural or commercial forces inhibiting this? We aren't aware of any. This Birds of a Feather session (BoF) attempted to identify existing barriers to the creation of such a framework, and attempted to identify the parties or groups which can contribute to the creation of a VO-powered data-publishing framework.

  11. A possibilistic approach for transient identification with 'don't know' response capability optimized by genetic algorithm

    International Nuclear Information System (INIS)

    This work describes a possibilistic approach for transient identification based on the minimum centroids set method, proposed in previous work, optimized by genetic algorithm. The idea behind this method is to split the complex classification problem into small and simple ones, so that the performance in the classification can be increased. In order to accomplish that, a genetic algorithm is used to learn, from realistic simulated data, the optimized time partitions, which the robustness and correctness in the classification are maximized. The use of a possibilistic classification approach propitiates natural and consistent classification rules, leading naturally to a good heuristic to handle the 'don't know 'response, in case of unrecognized transient, which is fairly desirable in transient classification systems where safety is critical. Application of the proposed approach to a nuclear transient indentification problem reveals good capability of the genetic algorithm in learning optimized possibilistic classification rules for efficient diagnosis including 'don't know' response. Obtained results are shown and commented. (author)

  12. ANALYSIS OF HEAVY METALS CONTENT IN PYLAISIELLA MOSS (PYLAISIA POLYANTHA GROWING IN THE CITY OF ROSTOV-ON-DON

    Directory of Open Access Journals (Sweden)

    Varduni T. V.

    2015-02-01

    Full Text Available The problem of environmental contamination by heavy metals is significant problem of urbanization. The search of ways of indication heavy metals pollution becomes actual, because of their simplicity and affordability. A widely used test objects for heavy metals pollution indicators are leafy mosses used for bioindication, because they are highly sensitive to any stress factor. The research shows that bioindication with using leafy mosses as test objects is highly effective method definition of heavy metals pollution. Using of bioindication methods are promising techniques for the assessment of the contamination of ecosystems by heavy metals. Through the use of this method, it is possible indication of pollution of the surface layer of air with heavy metals. The epiphytic moss (Pylaisia polyantha growing in different zones of the city of Rostov-on-Don, was used for the heavy metals pollution biomonitoring of urbosystems. The accumulation features of heavy metals in the epitaphic pylaisiella moss (Pylaisia polyantha in the territory of the city of Rostov-on-Don have been considered. pylaisiella moss (P. polyantha accumulates the largest amounts of the following heavy metals: Zn, Cr, Pb, Sr, Ni (Kc to 1.07, and Cu. According to the Kc values, all the studied elements accumulated by pylaisiella moss form the following series of biological uptake: Zn > Pb > Sr > Cr > V > Ni > Cu > Cо. The results of investigation showed that the epiphytic moss (Pylaisia polyantha can be used as indicator of heavy metals pollution in different polluted zones

  13. ECOLOGICAL STRESS AND ITS EFFECTS ON ESSENTIAL HYPERTENSION AND CHRONIC OBSTRUCTIVE BRONCHITIS DEVELOPMENT IN ROSTOV-ON-DON INHABITANTS

    Directory of Open Access Journals (Sweden)

    L. I. Katelnitskaya

    2015-12-01

    Full Text Available Aim. To study prevalence of essential hypertension (HT and chronic obstructive bronchitis (COB among Rostov-on-Don population, to assess influence of ecological stress on HT and COB development and to find the ways to improve therapy of combined pathology.Material and methods. Single-stage epidemiologic study of Rostov-on-Don population was carried out. The study included questionnaire to reveal main risk factors of chronic noninfectious diseases, a number of laboratory and instrumental indices.Results. Prevalence of HT and COB is significantly higher in patients, living in ecologically unfavorable districts. Rigidity and stretch ability of vascular wall influence the course and development of HT as well as atrial natriuretic factor. Carbonic anhydrase inhibitor, acetazolamide, when is added to standard therapy increases in its efficacy in blood pressure control, decrease in blood pressure in pulmonary artery and correction of disturbed acidbase balance in patients with HT combined with COB.Conclusion. The connection between unfavorable ecology and HT and COB development is demonstrated. Adjuvant therapy with acetazolamide is proposed to increase in efficacy of HT and COB standard therapy.

  14. Chemical Constituents of Picrasma quassioides (D.Don) Benn.%苦木化学成分研究

    Institute of Scientific and Technical Information of China (English)

    祝晨蔯; 邓贵华; 林朝展

    2012-01-01

    Five compounds were isolated and purified from the 95% ethanol extract of Picrasma quassioides (D. Don) Benn. by using column cinematography and recrystallization. On the basis of spectroscopic methods,their structures were identified as tiifolirhran (1) .maackiain (2) ,3',7-dihydroxy-4'-methoxyisoflavone (3) ,umbelliferone (4) ,and dmodin (5). All the Compounds are reported from the genus Picrasma for the first time.%运用各种柱色谱和重结晶等方法对苦木Pierasma quassioides (D.Don) Benn.进行分离纯化,得到五个化合物.通过NMR等现代波谱学方法和技术鉴定化合物的结构分别为:高丽槐素-3-0-β-D-葡萄糖苷(trifolirhizin,1),高丽槐素(maackiain,2),3',7-二羟基4'-甲氧基异黄酮(3',7 -dihydroxy-4'-methoxyisoflavone,3),7-羟基香豆素(ubelliferone,4)和大黄素(emodin,5).五个化合物均为首次从该属植物中分离得到.

  15. Live cell imaging in Drosophila melanogaster.

    Science.gov (United States)

    Parton, Richard M; Vallés, Ana Maria; Dobbie, Ian M; Davis, Ilan

    2010-04-01

    Although many of the techniques of live cell imaging in Drosophila melanogaster are also used by the greater community of cell biologists working on other model systems, studying living fly tissues presents unique difficulties with regard to keeping the cells alive, introducing fluorescent probes, and imaging through thick, hazy cytoplasm. This article outlines the major tissue types amenable to study by time-lapse cinematography and different methods for keeping the cells alive. It describes various imaging and associated techniques best suited to following changes in the distribution of fluorescently labeled molecules in real time in these tissues. Imaging, in general, is a rapidly developing discipline, and recent advances in imaging technology are able to greatly extend what can be achieved with live cell imaging of Drosophila tissues. As far as possible, this article includes the latest technical developments and discusses likely future developments in imaging methods that could have an impact on research using Drosophila. PMID:20360379

  16. Elemental analysis on the Myanma Tinospora Cordifolia (Hsin-Don-Ma-Nwe) by the energy dispersive X-ray fluorescence technique

    International Nuclear Information System (INIS)

    The application of the energy dispersive X-ray fluorescence technique by using high resolution X-ray detector system has been studied. The Myanma indigenous medicine Tinospora Cordifolia (Hsin-Don-Ma-Nwe) sample was analyzed by using quantitative analysis software (AXIL) provided by IAEA with personal computer

  17. "This Is My Body Which Will Be Given up for You": Federico Garcia Lorca's "Amor de Don Perlimplin" and the "Auto Sacramental" Tradition

    Science.gov (United States)

    Badenes, Jose Ignacio

    2009-01-01

    "Amor de Don Perlimplin con Belisa en su jardin" is one of Federico Garcia Lorca's shortest plays as well as one of his deepest. Its theme is love, particularly the interaction between spiritual longing and carnal desire, body and soul, humanity and divinity. These are aspects found in Roman Catholic Eucharistic theology which the…

  18. "Don Juan je psán pro Prahu - více netřeba říci"

    Czech Academy of Sciences Publication Activity Database

    Jonášová, Milada

    Praha : Národní divadlo, 2012 - (Blachut, B.), s. 13-28 ISBN 978-80-7258-383-6 R&D Projects: GA ČR GAP409/12/2563 Institutional support: RVO:68378076 Keywords : Mozart: Don Giovanni * Prague * opera Subject RIV: AL - Art, Architecture, Cultural Heritage

  19. Muusikamaailm : Praemium Imperiale kätteandmine. Vanamuusika päevad Hernes. Uus ooper Don Quijotest. Soome muusikud Münchenis / Priit Kuusk

    Index Scriptorium Estoniae

    Kuusk, Priit, 1938-

    1999-01-01

    Jaapani kunstide auhinna "Praemium Imperiale" laureaatidest. Lääne-Saksa Raadio festivalist "Herne", tänavu pealkirja all "Spiel auf, Europa!". C. Halffteri uudisooper "Don Quijote" tuleb maailmaesiettekandele Madridis 2000. a. veebruaris. Soome muusikute kontsertidest sügishooajal Baierimaal ja Münchenis

  20. Low soil water and nutrient availability below New Zealand kauri ( Agathis australis (D. Don) Lindl.) trees increase the relative fitness of kauri seedlings

    NARCIS (Netherlands)

    Verkaik, E.; Berendse, F.; Gardner, R.O.

    2007-01-01

    Tree species can affect the soil they are growing on and this might influence their fitness. The New Zealand gymnosperm tree species kauri (Agathis australis (D. Don) Lindl.) which grows in mixed angiosperm¿gymnosperm forests has a substantial effect upon the soil. We studied the hypotheses that: (1

  1. The Vanishing Mexicana/o: (Dis)Locating the Native in Ruiz de Burton's "Who Would Have thought It?" and "The Squatter and the Don"

    Science.gov (United States)

    Szeghi, Tereza M.

    2011-01-01

    This article complements the existing body of Ruiz de Burton scholarship by providing the first sustained examination of her literary representations of American Indians in both "Who Would Have Thought It?" (1872) and "The Squatter and the Don" (1885), and by exploring how these representations serve her broader aims of social and political…

  2. Scientific basis for use of Pyrus pashia Buch.-Ham. ex D. Don. fruit in gastrointestinal, respiratory and cardiovascular ailments.

    Directory of Open Access Journals (Sweden)

    Khalid Hussain Janbaz

    Full Text Available Pyrus pashia Buch.-Ham. ex D. Don. has been used conventionally by many communities in the Himalayan region for the management of gastrointestinal, respiratory, and vascular complications. Set against this background, this study was carried out to justify the scientific basis to validate folkloric uses of fruits of Pyrus pashia Buch.-Ham. ex D. Don. (Pp.Cr in traditional systems of medicine.The crude ethanol extract of fruits of Pyrus pashia Buch.-Ham. ex D. Don. (Pp.Cr was tested in vitro on isolated rabbit jejunum, tracheal, and aorta preparations. The responses of tissues were recorded using isotonic transducers coupled with a PowerLab data acquisition system.The Pp.Cr on application (0.01-5.0 mg/ml to isolated rabbit jejunum preparation exhibited relaxation through decrease in magnitude and frequency of spontaneous contractions. The Pp.Cr also exerted a relaxant (0.01-5.0 mg/ml effect on K+ (80 mM induced contractions in isolated rabbit jejunum preparations and caused shifting of the Ca2+ curves (1.0-3.0 mg/ml toward right in a manner similar to that of verapamil (3 μM, possibly suggesting presence of Ca2+ channel blocking activity. Subsequently, Pp.Cr in a concentration-dependent fashion (0.01-10.0 mg/ml caused relaxation of CCh (1 μM and K+ (80 mM induced contractions in isolated rabbit tracheal preparations in a manner comparable to that of dicyclomine, suggesting that the observed relaxant effect is likely to be mediated through antimuscarinic and/or Ca2+ channel blocking activities. Moreover, when evaluated against isolated rabbit aortic preparations, the Pp.Cr in concentrations up to 10 mg/ml exhibited a contractile response that was found to be abolished subsequent to pretreatment of isolated tissue preparation with cyproheptadine (1 μM, phentolamine (1 μM, and losartan (1 μM, suggesting that Pp.Cr may have some α-adrenergic, muscarinic, serotonergic, and angiotensin II activities.The aqueous ethanolic extract of Pyrus pashia

  3. Don Juan Vélez de León, refundidor de Quevedo (a propósito del romance “Don Repollo y doña Berza”)

    OpenAIRE

    Plata-Parga, F. (Fernando)

    2004-01-01

    Se estudian las repercusiones que tiene, para la cuestión de la autoría, el descubrimiento de nuevas versiones manuscritas del romance «Don Repollo y doña Berza» (número 683 en la edición de Blecua). El romance, publicado de forma anónima en 1621, ha sido atribuido en diferentes manuscritos a Góngora, Antonio Hurtado de Mendoza y a otros autores. La inclusión del romance en el Parnaso español (1648) de González de Salas adjudicó el poema de forma concluyente a Quevedo. Sin embargo, la recient...

  4. Isolation, characterisation and antibacterial activity studies of coumarins from Rhododendron lepidotum Wall. ex G. Don, Ericaceae Estudos de isolamento, caracterização e atividade antibacteriana de cumarinas de Rhododendron lepidotum Wall. ex G. Don, Ericaceae

    Directory of Open Access Journals (Sweden)

    Shakeel-U-Rehman

    2010-12-01

    Full Text Available Six coumarins daphnin (1, daphnetin (2, daphnetin glucoside (3, rhodonetin (4, rhodonin (5 and umbelliferone (6 were isolated from the methanolic extract of Rhododendron lepidotum Wall. ex G. Don, Ericaceae (aerial part. The compounds and their acetyl derivatives were screened for antibacterial activity against Staphylococcus aureus ATCC-29213, methicillin resistant Staphylococcus aureus ATCC-15187, Escherichia coli ATCC-8739, Pseudomonas aeruginosa ATCC-9027 by microdilution method as compared to the reference ciprofloxacin. Compound 2 displayed the best antibacterial activity with MIC 125 μg/mL against S. aureus ATCC-29213 and MRSA ATCC-15187 followed by 4 which exhibited the MIC value of 250 μg/mL against all the four tested strains. All molecules showed better antibacterial activity than their acyl derivatives.Seis cumarinas dafinina (1, dafinetina (2, dafinetina glicosídeo (3, rodonetina (4, rodonina (5 e umbeliferona (6 foram isoladas do extrato metanólico das partes aéreas de Rhododendron lepidotum Wall. ex G. Don, Ericaceae. Os compostos e seus derivados acetilados foram testados para verificar sua atividade antibacteriana contra Staphylococcus aureus ATCC-29213, Escherichia coli resistente à meticilina, Staphylococcus aureus ATCC-15187, ATCC-8739, Pseudomonas aeruginosa ATCC-9027, pelo método de microdiluição, usando ciprofloxacina como referência. A substância 2 apresentou a melhor atividade antibacteriana com o MIC 125 μg/mL contra S. aureus ATCC-29213 e MRSA ATCC-15187 seguido pela substância 4, que apresentou o valor de CIM de 250 μg/mL contra as quatro cepas testadas. Todas as moléculas apresentaram melhor atividade antibacteriana do que seus derivados acetilados.

  5. Minerales de mena del depósito epitermal de baja sulfuración Don Sixto, Mendoza Ore minerals from Don Sixto, a low sulfidation epithermal deposit from Mendoza

    Directory of Open Access Journals (Sweden)

    Ana Cecilia Mugas Lobos

    2012-03-01

    Full Text Available El proyecto minero Don Sixto es un depósito epitermal de baja sulfuración de Au-Ag, ubicado en el bloque de San Rafael, en el SE de la provincia de Mendoza. En este depósito, cuyo recurso aurífero es próximo a las 900.000 onzas, se ha identificado una asociación de minerales de metales preciosos que incluye: uytenbogaardtita, stromeyerita, naumannita, acantita, polibasita, stützita y cervelleita. Estos minerales son escasos y se encuentran frecuentemente asociados a oro y plata de variable fineza, diseminados en vetas de cuarzo hidrotermal y en unidades volcánicas-piroclásticas de los Grupos Choiyoi y El Portillo. En este depósito se encuentra abundante pirita, con cantidades menores de arsenopirita, calcopirita, esfalerita, magnetita, hematita y, en forma subordinada, pirrotina, galena, marcasita, bornita y boulangerita, junto con calcosina, digenita y covellina. La presencia de uytenbogaardtita, stützita, cervelleita y polibasita constituye la primera mención de estos minerales para la provincia de Mendoza.The Don Sixto mining project is a low sulfidation Au-Ag deposit, with a gold resource close to 900,000 ounces, located in the San Rafael Massif, in the SE of Mendoza province. Recently, it was found a precious-metal mineral association including uy-tenbogaardtite, stromeyerite, naumannite, acanthite, polybasite, stützite and cervelleite. These minerals are scarce and are commonly related to gold and silver of variable fineness, disseminated in hydrothermal quartz veins and volcanic-pyroclastic rock units from the Choiyoi and El Portillo Group. The most common mineral is pyrite associated with subordinated arsenopyrite, chalcopyrite, sphalerite, magnetite and hematite; lesser amounts of pyrrhotite, galena, marcasite, bornite, boulangerite, chalcocite, digenite and covellite were also recognized. This is the first mention in Mendoza province for uytenbogaardtite, stützite, cervelleite and polybasite.

  6. WOUND HEALING ACTIVITY OF ALCOHOLIC EXTRACT OF SOLANUM ERIANTHUM D.DON IN EXCISION AND INCISION METHOD

    Directory of Open Access Journals (Sweden)

    Ch. Sudha Bhargavi

    2013-02-01

    Full Text Available Solanum erianthum D. Don is an unarmed shrub or small tree with dense indumentums of soft stellate hairs. Leaves are simple, ovate-elliptical; margin entire or slight wavy, base rounded to cuneate, and apex acute to acuminate. The leaves have been extensively used for leucorrhoea, piles, hemorrhoids, scrofula, headache, vertigo, digestive troubles and for wound healing purposes. Wistar albino rats of either sex weighing between 200 and 220g were topically treated with extract formulated in gel (10% and 20% which was applied once daily in excision and incision wound model. Rats of standard groups were treated with 5% w/w Povidone-Iodine ointment topically. The percentage of wound contraction was increased, epithelization period was decreased and wound breaking strength was increased with topical application of AESE (Alcoholic Extract of Solanum erianthum gel in excision and incision wound model. The experimental data revealed that the AESE displayed remarkable wound healing activity thus supports its traditional use.

  7. Don Draper, avatares e twittertainment: o comportamento dos fãs de TV na era transmidiática

    OpenAIRE

    Neves, Sheron

    2011-01-01

    O artigo propõe examinar o comportamento dos fãs de TV no atual ambiente transmidiático, usando como referência a série norte-americana Mad Men e a transformação do personagem Don Draper em celebridade. Pretendo examinar o impacto das transformações culturais sobre as estratégias de marketing das empresas produtoras de conteúdos, já que, à medida que os meios convergem, os fãs se apropriam de personagens e marcas. À medida que os fãs são constantemente convidados a imergir em universos imagin...

  8. Genotoxicity and contamination of natural and anthropogenically transformed soils of the city of Rostov-on-Don with heavy metals

    Science.gov (United States)

    Gorbov, S. N.; Bezuglova, O. S.; Varduni, T. V.; Gorovtsov, A. V.; Tagiverdiev, S. S.; Hildebrant, Yu. A.

    2015-12-01

    The integrated characteristics of urban soils included the assessment of heavy metal pollution linked to the determination of soil genotoxicity, which characterizes the soil capacity to affect the structural and functional state of the genetic apparatus of soil biota. Increased concentration of chromium caused by the high background level was found in soils of the city of Rostov-on-Don. A weak contamination of surface horizons with lead and arsenic was also noted. Maximum permissible concentration of zinc was locally exceeded, including deep soil horizons. It was found that there is no definite correlation between the total content of heavy metals, their mobile compounds, and the parameters of genotoxicity in the natural and anthropogenically transformed soils. It was concluded that soil genotoxicity characterizes the total mutagenic activity associated with the presence of a set of genotoxicants of different nature.

  9. Living in a simulacrum: how TV and the supermarket redefines reality in Don DeLillo’s White Noise

    Directory of Open Access Journals (Sweden)

    Ghashmari, Ahmad

    2010-07-01

    Full Text Available This paper examines the impact of simulation, hyperreality, and consumerism on Don Delillo's novel "White Noise". It discusses how the novel pictures technology and mass media as an empire of signs and codes that erase or implode meaning. TV, radio reports and tidbits, and medical imaging devices are intertwined with many aspects of people's lives in this late capitalist culture. Futhermore, the paper will shed some light on the issue of hyperreality which is generated by simulations. We will see how this new type of reality becomes more real than reality itself. Then, the influence of the supermarket and the emergence of consumer culture will be discussed. We will see how production and consumption have gained a new different meaning in this new "superficial" society and how it reshapes people's undestanding and interaction with reality.

  10. Morphobiochemical Variability and Selection Strategies for the Germplasm of Dactylorhiza hatagirea (D. Don Soo: An Endangered Medicinal Orchid

    Directory of Open Access Journals (Sweden)

    R. S. Chauhan

    2014-01-01

    Full Text Available Dactylorhiza hatagirea (D. Don Soo (Orchidaceae is an important endangered medicinal herb, distributed in subalpine to alpine regions of the Himalayas. Its tubers are important constituents of many medicines and health tonics. Overexploitation for medicinal uses has decreased availability in natural habitats and this species has been enlisted as endangered, making conservation and cultivation studies necessary. Variability studies may serve as an important tool for effective conservation and for a crop improvement program. Therefore, natural populations of D. hatagirea were analyzed for variability on the basis of morphological, biochemical, and isoenzyme patterns. The studied populations were grouped into two clusters. Existing variability among different populations opens up new areas for conservation and perspectives for a genetic improvement program for D. hatagirea.

  11. Geological and environmental observations in the Sunchales Creek and Tonco River , Don Otto uranium mine, Salta Province

    International Nuclear Information System (INIS)

    Results of geological and environmental observations made by professionals of the Comisión Nacional de Energía Atómica (CNEA) down streams Don Otto uranium mine on the Sunchales creek, from Los Guanacos strait down to the Tonco river mouth, and from river mouth down to narrow pass named Salto Grande, are presented in this report. La Yesera, Las Curtiembres and Los Blanquitos Formations of the Pirgua Subgroup, and Lecho and Yacoraite Formations of the Balbuena Subgroup crops out in that area (Salta Group, Cretaceous- Middle Eocene). Water stream analyses have given uranium contents between 890 μg/L and 1,100 μg/L, and uranium in stream sediments between 4 μg/g and 14 μg/g; radimetric anomalias up to 1,500 c/s in discoloured facies of the Las Curtiembres Formation, and 2,000 c/s in dark pelitic sediments of the Yacoraite Formation. A new zone with uranium traces in Curtiembres Formation belonging to the Pirgua Subgroup has been identified in the Alemanía cretaceous basin, which is stratigraphically similar to Cajoncillo, Las Abritas and El Fraile deposits. Those traces in addition to the other ones localized in the upper third of the Yacoraite Formation would have been originated by the geochemical uranium anomalies in waters and stream sediments by uranium leaching of rocks. The geochemical uranium anomalies in water and sediments are not a consequence of the antropic activity of the uranium mining carried out in Don Otto mine (1961-1982). (authors)

  12. Suitport Feasibility - Human Pressurized Space Suit Donning Tests with the Marman Clamp and Pneumatic Flipper Suitport Concepts

    Science.gov (United States)

    Boyle, Robert M.; Rodriggs, Liana; Allton, Charles; Jennings, Mallory; Aitchision, Lindsay

    2013-01-01

    The suitport concept has been recently implemented as part of the small pressurized lunar rover (Currently the Space Exploration vehicle, or SEV) and the Multi-Mission Space Exploration Vehicle (MMSEV) concept demonstrator vehicle. Suitport replaces or augments the traditional airlock function of a spacecraft by providing a bulkhead opening, capture mechanism, and sealing system to allow ingress and egress of a space suit while the space suit remains outside of the pressurized volume of the spacecraft. This presents significant new opportunities to EVA exploration in both microgravity and surface environments. The suitport concept will enable three main improvements in EVA by providing reductions in: pre-EVA time from hours to less than thirty minutes; airlock consumables; contamination returned to the cabin with the EVA crewmember. Two second generation suitports were designed and tested. The previously reported second generation Marman Clamp suitport and a newer concept, the Pneumatic Flipper Suitport. These second generation suitports demonstrated human donning and doffing of the Z1 spacesuit with an 8.3 psi pressure differential across the spacesuit. Testing was performed using the JSC B32 Chamber B, a human rated vacuum chamber. The test included human rated suitports, the suitport compatible prototype suit, and chamber modifications. This test brought these three elements together in the first ever pressurized donning of a rear entry suit through a suitport. This paper presents the results of the testing, including unexpected difficulties with doffing, and engineering solutions implemented to ease the difficulties. A review of suitport functions, including a discussion of the need to doff a pressurized suit in earth gravity, is included. Recommendations for future design and testing are documented.

  13. LA PARODIA ENTRE LA FICCIÓN YLA REALIDAD EN EL INGENIOSO HIDALGO DON QUIJOTE DE LA MANCHA

    Directory of Open Access Journals (Sweden)

    Nora González Gandiaga

    2005-11-01

    Full Text Available En este trabajo se aborda la problemática de la ficción y de la realidad intermediada por la operación paródica en El Ingenioso Hidalgo don Quijote de La Mancha de Miguel de Cervantes Saavedra. Se asedia el texto para ver cómo la parodia y la ironía son estrategias fundamentales para leer la concepción novelesca y cómo se permite al lector avieso adentrarse en la Poética cervantina, partiendo de los paratextos: prólogo, sonetos iniciales antes de adentrarnos en el cuerpo de la novela para luego calar en éste. En todo este cometido colaboran los narradores ficticios para "leer" la novela moderna que postula Cervantes en el libro e incluso se advierte que los distintos narradores dejan al descubierto la parodia y al mismo tiempo permiten un juego crítico-reflexivo en la materia textualThis paper examines the problematic of fiction and reality as mediated by parody in Cervantes’ Don Quijote de la Mancha. The approach to the text is meant to see how parody and irony become fundamental strategies to understand the novelistic concept, and how the perceptive reader is permitted to enter into the Cervantine poetic, starting from the paratexts: the Prologue; the opening sonnets, before getting into the core of the work discriminatingly. In all this procedure fictitious narrators collaborate in the reading of the modernistic novel which Cervantes postulated. It may also be noticed how the different narrators disclose the parodic while allowing a critical-reflexive play in the text

  14. Amperometric biosensor with nanostructured electrodes by using multi-alled carbon nanotubes for glucose detection in cell culture medium

    OpenAIRE

    Boero, Cristina; Carrara, Sandro; De Micheli, Giovanni

    2009-01-01

    The monitoring of metabolic compounds such as glucose is largely reported in literature. The applications of this type of analysis are mainly related to clinical purposes, e.g. in diabetes pathology, where a lot of studies are presented in literature. Recently, some authors presented studies about glucose and lactate detection in cell culture monitoring [1], [2]. A clear identification of medium compounds could be interesting for biologists and biotechnologists, since they may be identified a...

  15. Moderniųjų jausmų sociogenezė: meilė ir draugystė Miguelio de Cervanteso “Don Kichote” ir Williamo Shakespeare’o “Romeo ir Džuljetoje” | The sociogenesis of modern feelings: love and friendship in Miguel de Cervantes’ “Don Quixote” and William Shakespeare’s “Romeo and Juliet”

    OpenAIRE

    Leonidas Donskis

    2006-01-01

    William Shakespeare’s “Romeo and Juliet” reveals modern feelings – love and friendship – and their sociogenesis. Love and friendship emerge here as the feelings of a modern person. “Don Quixote of La Mancha”, the great novel by Miguel de Cervantes Saavedra, to this day keeps providing new insights and hot moral and philosophical issues for debate. “Don Quixote” is closely related to the great paradoxes of life: how to sustain our moral code in a world where morality is vanishing; how to susta...

  16. Peut-on penser le travail par le don ? Débat avec la rédaction de la Nouvelle Revue du Travail Conceptualising work as a gift? Debate with editors of the Nouvelle Revue du Travail ¿Es posible pensar el trabajo a través del don? Debate con el comité de redacción de la Nouvelle Revue du Travail

    OpenAIRE

    Alain Caillé; Norbert Alter

    2012-01-01

    Dans un échange avec la Nouvelle Revue du Travail, Alain Caillé et Norbert Alter soutiennent leur lecture maussienne des réalités contemporaines du travail en discutant les questions centrales du capitalisme, du salariat, de l’entreprise et du travail sociologique. Cet échange fait suite aux contributions d’Alain Caillé et Norbert Alter dans ce Chorus « Peut-on penser le travail par le don ? De la mise en œuvre du paradigme du don contre-don en sociologie du travail. »In an exchange with the ...

  17. Final Environmental Assessment : Opening waterfowl hunting on 5,500 acres of former commercial salt ponds : Don Edwards San Francisco Bay National Wildlife Refuge : Alviso and Ravenswood Salt Ponds

    Data.gov (United States)

    US Fish and Wildlife Service, Department of the Interior — This EA for opening waterfowl hunting on salt ponds recently acquired by the Don Edwards San Francisco Bay National Wildlife Refuge proposes three alternatives....

  18. Electron Tomography in Plant Cell Biology

    Institute of Scientific and Technical Information of China (English)

    2007-01-01

    This review focuses on the contribution of electron tomography-based techniques to our understanding of cellular processes in plant cells. Electron microscopy techniques have evolved to provide better three-dimensional resolution and improved preservation of the subcellular components. In particular, the combination of cryofixation/freeze substitution and electron tomography have allowed plant cell biologists to image organelles and macromolecular complexes in their native cellular context with unprecedented three-dimensional resolution (4-7 nm). Until now, electron tomography has been applied in plant cell biology for the study of cytokinesis, Golgi structure and trafficking, formation of plant endosome/prevacuolar compartments, and organization of photosynthetic membranes. We discuss in this review the new insights that these tomographic studies have brought to the plant biology field.

  19. Endosymbiosis, cell evolution, and speciation.

    Science.gov (United States)

    Kutschera, U; Niklas, K J

    2005-08-01

    In 1905, the Russian biologist C. Mereschkowsky postulated that plastids (e.g., chloroplasts) are the evolutionary descendants of endosymbiotic cyanobacteria-like organisms. In 1927, I. Wallin explicitly postulated that mitochondria likewise evolved from once free-living bacteria. Here, we summarize the history of these endosymbiotic concepts to their modern-day derivative, the "serial endosymbiosis theory", which collectively expound on the origin of eukaryotic cell organelles (plastids, mitochondria) and subsequent endosymbiotic events. Additionally, we review recent hypotheses about the origin of the nucleus. Model systems for the study of "endosymbiosis in action" are also described, and the hypothesis that symbiogenesis may contribute to the generation of new species is critically assessed with special reference to the secondary and tertiary endosymbiosis (macroevolution) of unicellular eukaryotic algae. PMID:17046345

  20. Detrital control on the release of dissolved organic nitrogen (DON) and dissolved inorganic nitrogen (DIN) from the forest floor under chronic N deposition.

    Science.gov (United States)

    Park, Ji-Hyung; Matzner, Egbert

    2006-09-01

    The role of detrital quantity and quality in forest floor N leaching was investigated in a litter manipulation experiment at a deciduous forest under chronic N deposition. Dissolved inorganic nitrogen (DIN) comprised the bulk of nitrogen leaching from the control except a short period following autumn litterfall. The dominance of DIN was strengthened by litter exclusion, whereas the addition of glucose or fresh litter led to a small increase in dissolved organic nitrogen (DON) and either a temporary or gradual reduction in NO(3)(-) release, respectively. Changes in soluble organic C and microbial C in the forest floor implied that increased availability of C sources might have enhanced microbial immobilization of DIN, either temporarily following glucose application or over the longer term following litter addition. The results suggest that detrital quantity and quality can play a crucial role in determining the balance between DIN and DON in N-enriched forest soils. PMID:16406164

  1. Savez-vous à qui vous avez affaire ? De la vengeance au don, de l’offense au pardon

    Directory of Open Access Journals (Sweden)

    Mark R. Anspach

    2009-02-01

    Full Text Available Pardonner, c’est renoncer au désir de se venger, c’est donc éviter de s’engager dans le cercle de la réciprocité négative. Or, il est difficile d’échapper à ce cercle vicieux sans accepter d’entrer dans le cercle vertueux de la réciprocité positive : voilà du moins la thèse que nous avons soutenue dans un premier travail sur la violence et le don. Nous nous proposons ici de partir de cette même thèse pour entamer une nouvelle réflexion sur le pardon. Comment le passage de la vengeance au don ...

  2. Polycyclic aromatic hydrocarbon levels and measures of oxidative stress in the Mediterranean endemic bivalve Pinna nobilis exposed to the Don Pedro oil spill.

    Science.gov (United States)

    Sureda, Antoni; Tejada, Silvia; Box, Antonio; Deudero, Salud

    2013-06-15

    The fan mussel (Pinna nobilis Linné, 1758) is the largest endemic Mediterranean bivalve subject to strict protection as an endangered species. Antioxidant biomarkers in P. nobilis gills for biomonitoring marine pollution were researched after the Don Pedro oil spill. Two sampling locations on the east and southeast of the island of Ibiza (Western Mediterranean, Spain) were selected, one extensively affected by the oil spill and the other unaffected (control area). Mussels were sampled 1 month, 6 months and 1 year after the accident. Polycyclic aromatic hydrocarbon levels and antioxidant enzymes significantly increased as result of the oil spill in all sampling periods (p<0.05). Oxidative damage in lipids significantly increased in the mussels collected in the affected area (p<0.05), though such damage was back to normal after 1 year. In conclusion, the Don Pedro oil spill induced a situation of oxidative stress on P. nobilis that continued a year later. PMID:23623655

  3. "It sure as hell looked like war": terrorism and the Cold War in Thomas Pynchon's Against the Day and Don DeLillo's Underworld

    OpenAIRE

    Eve, Martin Paul

    2013-01-01

    This piece explores, necessarily briefly, the conceptions of terrorism in two novels that stand separated by the calamitous events of September 11th, 2001: Pynchon's Against the Day and Don DeLillo's Underworld, with special focus upon the genesis of these depictions in Cold War politics. While there are cases to be made for many geographico-historical connections in both Pynchon's and DeLillo's work ? for instance, Sam Thomas has recently highlighted the Balkans ? the Cold War presents a loc...

  4. Evaluation of the in vitro and in vivo antioxidant potential of whole plant extracts of Hedyotis puberula (G. Don) R. Br. ex Arn.

    OpenAIRE

    Joseph, Jince M.; Sowndhararajan, Kandhasamy; Manian, Sellamuthu

    2011-01-01

    The total phenolic content and in vitro and in vivo antioxidant activities of the whole plants of Hedyotis puberula (G. Don) R.Br. ex Arn. were appraised. The methanol extract of the plant contained higher levels of total phenolics, tannins and flavonoids content than other solvent extracts. Extracts were screened for antioxidant and free radical scavenging activities using various in vitro model systems. The methanol extract manifested strongest antioxidant and free radical scave...

  5. Short-term and long-term effects of tannins on nitrogen mineralisation and litter decomposition in kauri (Agathis australis (D. Don) Lindl.) forests

    OpenAIRE

    Verkaik, E.; Jongkind, A.G.; Berendse, F.

    2006-01-01

    Kauri (Agathis australis (D. Don) Lindl.) occurs naturally in the warm temperate forest of northern New Zealand where it grows mixed with angiosperm tree species. Below mature kauri trees thick organic layers develop in which large amounts of nitrogen are accumulated. This nitrogen seems to be inaccessible to plants. While litter quality can explain the low decomposition rate below kauri, it is not known what causes the accumulation of nitrogen. We hypothesised that kauri tannins reduce nitro...

  6. USING MINDMAPPING TECHNIQUE TO INCREASE THE ABILITY IN WRITING NEWS REPORT AT THE ELEVENTH GRADE STUDENTS OF IPA AT SMA FRATER. DON BOSCO

    OpenAIRE

    Jerry Mias Rofinus

    2011-01-01

    Jerry Mias Rofinus 2011. Using Mind Mapping technique to Increase the Ability in Writing News Report at the Eleventh Grade Students of IPA at SMA Frater Don Bosco Tarakan. Pembimbing: (1) Yansar, S.S., M. Pd (2) Ridwan, M. Pd) In learning process, there were domains that expected could help in achievement by the objective learning. The three of domain are cognitive, affective and psychometric. The focus given to third domain and expected can improve performance by students. In fact, in lea...

  7. Stratigraphic and tectonic notes on the Villafranca d'Asti succession in type-area and Castelnuovo Don Bosco sector (ASTI reliefs, Piedmont)

    OpenAIRE

    Forno, GM; GATTIGLIO, M.; C. Comina; BARBERO, D.; Bertini, A; Doglione, A; F. Gianotti; Irace, A.; Martinetto, E.; Mottura, A; Sala, B.

    2015-01-01

    Interest in the sedimentary succession in the Asti Reliefs fostered research since the beginning of the science of geology, before the designation by Pareto (1865) of the "Piano Villafranchiano" and its type area at Villafranca d'Asti. The villafranchian sediments have been the subject of many recent investigations, which focused largely on the palaeontology of vertebrates and plant remains. Geological surveys in the type area and in the Castelnuovo don Bosco sector revealed that the sediment...

  8. Evaluating the conservation impact of an innovative zoo-based educational campaign: ‘Don't Palm Us Off’ for orang-utan conservation

    OpenAIRE

    Pearson, Elissa L; Lowry, Rachel; Dorrian, Jillian; Carla A. Litchfield

    2014-01-01

    With significant biodiversity loss occurring presently, increased emphasis is being placed upon the capacity of zoos to contribute to species conservation. This paper evaluates an innovative conservation education campaign ‘Don't Palm Us Off’ implemented at Melbourne Zoo, Australia. This sought to address a lack of public awareness regarding palm oil (the product most threatening the survival of the orang-utan) and to create public support for mandatory labeling of palm oil on food products, ...

  9. Epidemiology of Ancylostoma spp. in the endangered Iberian lynx (Lynx pardinus) in the Don˜ ana National Park, south-west Spain

    OpenAIRE

    Vicente, J. de; Palomares, Francisco; Ruíz de Ibáñez, R.; Ortíz, J.

    2004-01-01

    The epidemiology of Ancylostoma spp. was studied in the endangered Iberian lynx (Lynx pardinus) in the Don˜ ana National Park, south-west Spain. Faecal samples were collected throughout a complete annual cycle (August 1997 to September 1998). The overall egg prevalence of Ancylostoma spp. was 57.8%. The pattern of abundance of Ancylostoma spp. eggs in faeces was overdispersed. Juvenile lynx demonstrated a statistically higher prevalence and abundance of Ancylostoma spp. than i...

  10. Anotaciones sobre la conformación del patrimonio de don José de Castellví, marqués de Villatorcas (1653-1722)

    OpenAIRE

    Felipo Orts, Amparo

    2009-01-01

    En estas páginas presentamos unos apuntes sobre el proceso de conformación y consolidación del patrimonio de don José de Castellví, marqués de Villatorcas. A tal fin, rastreamos los mecanismos de transmisión de los bienes de sus antepasados, indagamos en las adquisiciones personales y profundizamos en el inventario de los bienes que a su muerte en 1722 se conservaban en Valencia. (A)

  11. El canónigo don José de Castellvi y Coloma. Entre el ministerio eclesiástico y la defensa del patrimonio familiar

    OpenAIRE

    Felipo Orts, Amparo

    2013-01-01

    Se analiza la personalidad de don José de Castellví y Coloma y cómo las repercusiones de la Guerra de Sucesión sobre su familia le convirtieron en un personaje clave para la preservación del patrimonio familiar,en el custodio de la importante biblioteca de su padre, el marqués de Villatorcas, y en mediador entre los hermanos en su enfrentamiento por la herencia paterna.

  12. The Islamic Drug Prevention Method among Thai Muslim Youths in Ban Don Khilek, Phawong Sub-District, Muang District, Songkhla Province

    OpenAIRE

    Rutchadee Binwang; Kasetchai Laeheem

    2013-01-01

    This study aimed to investigate a method and effects of community participation in an Islamic drug prevention method among Thai Muslim youths in Ban Don Khilek, Tambon Phawong, Muang District, Songkhla Province. The data were collected through in-depth interviews, group discussions, and practice with four groups of key informants, namely, 20 Muslim youths, 10 parents and guardians, 8 religious leaders, and 3 local leaders. Descriptive content analysis was conducted using comparative logic of ...

  13. Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don

    OpenAIRE

    Shi-Hai Xing; Xin-Bo Guo; Quan Wang; Qi-Fang Pan; Yue-Sheng Tian; Pin Liu; Jing-Ya Zhao; Guo-Feng Wang; Xiao-Fen Sun; Ke-Xuan Tang

    2011-01-01

    The tetraploid plants of Catharanthus roseus (L.) G. Don was obtained by colchicine induction from seeds explants, and the ploidy of the plants was identified by flow cytometry. The optimal treatment is 0.2% colchicine solution treated for 24 hours, and the induction rate reaches up to 30%. Comparing with morphological characteristics and growth habits between tetraploids and the control, we found that tetraploids of C. roseus had larger stoma and more branches and leaves. HPLC analysis showe...

  14. Suitport Feasibility - Development and Test of a Suitport and Space Suit for Human Pressurized Space Suit Donning Tests

    Science.gov (United States)

    Boyle, Robert M.; Mitchell, Kathryn; Allton, Charles; Ju, Hsing

    2011-01-01

    The suitport concept has been recently implemented as part of the small pressurized lunar rover (Currently the Space Exploration vehicle, or SEV) and the Multi-Mission Space Exploration Vehicle (MMSEV) concept demonstrator vehicle. Suitport replaces or augments the traditional airlock function of a spacecraft by providing a bulkhead opening, capture mechanism, and sealing system to allow ingress and egress of a spacesuit while the spacesuit remains outside of the pressurized volume of the spacecraft. This presents significant new opportunities to EVA exploration in both microgravity and surface environments. The suitport concept will enable three main improvements in EVA by providing reductions in: pre-EVA time from hours to less than thirty minutes; airlock consumables; contamination returned to the cabin with the EVA crewmember. To date, the first generation suitport has been tested with mockup suits on the rover cabins and pressurized on a bench top engineering unit. The work on the rover cabin has helped define the operational concepts and timelines, and has demonstrated the potential of suitport to save significant amounts of crew time before and after EVAs. The work with the engineering unit has successfully demonstrated the pressurizable seal concept including the ability to seal after the introduction and removal of contamination to the sealing surfaces. Using this experience, a second generation suitport was designed. This second generation suitport has been tested with a spacesuit prototype using the pressure differentials of the spacecraft. This test will be performed using the JSC B32 Chamber B, a human rated vacuum chamber. This test will include human rated suitports, the suitport compatible prototype suit, and chamber modifications. This test will bring these three elements together in the first ever pressurized donning of a rear entry suit through a suitport. This paper presents design of a human rated second generation suitport, modifications to

  15. Suitport Feasibility: Development and Test of a Suitport and Space Suit for Human Pressurized Space Suit Donning Tests

    Science.gov (United States)

    Boyle, Robert M.; Mitchell, Kathryn; Allton, Charles; Ju, Hsing

    2012-01-01

    The suitport concept has been recently implemented as part of the small pressurized lunar rover (Currently the Space Exploration vehicle, or SEV) and the Multi-Mission Space Exploration Vehicle (MMSEV) concept demonstrator vehicle. Suitport replaces or augments the traditional airlock function of a spacecraft by providing a bulkhead opening, capture mechanism, and sealing system to allow ingress and egress of a space suit while the space suit remains outside of the pressurized volume of the spacecraft. This presents significant new opportunities to EVA exploration in both microgravity and surface environments. The suitport concept will enable three main improvements in EVA by providing reductions in: pre-EVA time from hours to less than thirty minutes; airlock consumables; contamination returned to the cabin with the EVA crewmember. To date, the first generation suitport has been tested with mockup suits on the rover cabins and pressurized on a bench top engineering unit. The work on the rover cabin has helped define the operational concepts and timelines, and has demonstrated the potential of suitport to save significant amounts of crew time before and after EVAs. The work with the engineering unit has successfully demonstrated the pressurizable seal concept including the ability to seal after the introduction and removal of contamination to the sealing surfaces. Using this experience, a second generation suitport was designed. This second generation suitport has been tested with a space suit prototype on the second generation MMSEV cabin, and testing is planned using the pressure differentials of the spacecraft. Pressurized testing will be performed using the JSC B32 Chamber B, a human rated vacuum chamber. This test will include human rated suitports, a suitport compatible prototype suit, and chamber modifications. This test will bring these three elements together in the first ever pressurized donning of a rear entry suit through a suitport. This paper presents

  16. 秋水仙素诱导川贝母(Fritillaria cirrhosa D.Don)愈伤组织多倍体的研究%Induction of Polyploid from Colchicine-Treated Fritillaria cirrhosa D.Don Callus

    Institute of Scientific and Technical Information of China (English)

    王强; 兰利琼; 傅华龙

    2002-01-01

    应用组织培养技术对川贝母(Fritillaria cirrhosa D.Don)进行多倍体诱导.结果表明:组织培养条件下,将川贝母愈伤组织在培养基中添加一定浓度的秋水仙素处理一段时间,或者经一定浓度的秋水仙素浸泡一段时间后再培养,均可诱发川贝母多倍体的产生,但以前者效果较好,在秋水仙素浓度为1 000 mg/L,处理5 d的条件下,诱导率最高达70%.细胞染色体鉴定结果为:四倍体染色体数为2n=4x=48,而二倍体的染色体数为2n=2x=24.

  17. Don't fence me in: Harnessing the beneficial roles of astrocytes for spinal cord repair

    OpenAIRE

    White, Robin E.; Jakeman, Lyn B.

    2008-01-01

    Astrocytes comprise a heterogeneous cell population that plays a complex role in repair after spinal cord injury. Reactive astrocytes are major contributors to the glial scar that is a physical and chemical barrier to axonal regeneration. Yet, consistent with a supportive role in development, astrocytes secrete neurotrophic factors and protect neurons and glia spared by the injury. In development and after injury, local cues are modulators of astrocyte phenotype and function. When multipotent...

  18. Detrital control on the release of dissolved organic nitrogen (DON) and dissolved inorganic nitrogen (DIN) from the forest floor under chronic N deposition

    Energy Technology Data Exchange (ETDEWEB)

    Park, Ji-Hyung [Department of Soil Ecology, University of Bayreuth, D-95440 Bayreuth (Germany)]. E-mail: jihyungpark@yahoo.com; Matzner, Egbert [Department of Soil Ecology, University of Bayreuth, D-95440 Bayreuth (Germany)

    2006-09-15

    The role of detrital quantity and quality in forest floor N leaching was investigated in a litter manipulation experiment at a deciduous forest under chronic N deposition. Dissolved inorganic nitrogen (DIN) comprised the bulk of nitrogen leaching from the control except a short period following autumn litterfall. The dominance of DIN was strengthened by litter exclusion, whereas the addition of glucose or fresh litter led to a small increase in dissolved organic nitrogen (DON) and either a temporary or gradual reduction in NO{sub 3} {sup -} release, respectively. Changes in soluble organic C and microbial C in the forest floor implied that increased availability of C sources might have enhanced microbial immobilization of DIN, either temporarily following glucose application or over the longer term following litter addition. The results suggest that detrital quantity and quality can play a crucial role in determining the balance between DIN and DON in N-enriched forest soils. - Detrital quantity and quality play a crucial role in regulating the release of DON and DIN from the forest floor under chronic N deposition.

  19. Changes in the Subpopulations of Porcine Peripheral Blood Lymphocytes Induced by Exposure to Low Doses of Zearalenone (ZEN) and Deoxynivalenol (DON).

    Science.gov (United States)

    Dąbrowski, Michał; Obremski, Kazimierz; Gajęcka, Magdalena; Gajęcki, Maciej Tadeusz; Zielonka, Łukasz

    2016-01-01

    Zearalenone and deoxynivalenol are secondary metabolites of fungi of the genus Fusarium. The presence of mycotoxins in cereals and the resulting contamination of feeds and foods pose health risks for animals and humans. The dangers associated with high doses of mycotoxins have been extensively researched but very little is known about NOAEL (No Observed Adverse Effect Level) doses or exposure to a combination of mycotoxins (mixed mycotoxicoses). The aim of this study was to determine the effects of six-week exposure to NOAEL doses of individual and combined mycotoxins on the subpopulations of CD4⁺8(-), CD4(-)8⁺ and CD4⁺8⁺ lymphocytes in the peripheral blood of pigs. The experiment was performed on 72 gilts with average body weight of 25 kg, divided into three experimental groups (E1, E2 and E3, administered zearalenone (ZEN), deoxynivalenol (DON) and ZEN + DON, respectively, on a daily basis) and a control group (C) receiving placebo. Changes in lymphocyte subpopulations were evaluated by flow cytometry at weekly intervals (experimental days 7, 14, 21, 28, 35 and 42). A linear increase in the percentage of CD4⁺8⁺ lymphocytes was highly correlated with time (r = 0.682) in group C. The correlations and linear increase in the above subpopulation were disrupted in the remaining groups. In group E3, a statistically significant (p ZEN and DON exerted stronger immunomodulatory effects. PMID:27128894

  20. Nietzsche e Dom Quixote: diálogos entre ‘loucos’ = Nietzsche and Don Quixote: dialogues between ‘mad’

    Directory of Open Access Journals (Sweden)

    Helder Felix Pereira Souza

    2012-01-01

    Full Text Available Partindo das ideias do pensador Nietzsche e caminhando por diversas obras escritas pelo pensador, selecionaram-se algumas passagens para projetar olhares sobre a obra literária Dom Quixote de la Mancha, do espanhol Miguel de Cervantes, sobretudo, em relação à personagem Dom Quixote. Possibilita-se, assim, apresentar outras perspectivas sobre essas duas peculiares figuras: o pensador Nietzsche e o cavaleiro andante Dom Quixote. Destaca-se, por fim, a importância deste entrecruzamento para estimular possibilidades imaginativas-criativas para se pensar a filosofia e a arte mediante a exceção, a loucura e o devir.Building on the ideas of philosopher Nietzsche and walking on several works written by the thinker, some passages have been selected to design looks on the literary work Don Quixote de la Mancha, of Spanish, Miguel de Cervantes, especially in relation to the character Don Quixote. Thus making it possible to present other perspectives on these two unique figures: the thinker Nietzsche and the knight-errant Don Quixote. Emphasizing, finally, the importance of this intersection to stimulate possibilities imaginative-creative of thinking the philosophy and art through the exception, of madness and of devir.

  1. Detrital control on the release of dissolved organic nitrogen (DON) and dissolved inorganic nitrogen (DIN) from the forest floor under chronic N deposition

    International Nuclear Information System (INIS)

    The role of detrital quantity and quality in forest floor N leaching was investigated in a litter manipulation experiment at a deciduous forest under chronic N deposition. Dissolved inorganic nitrogen (DIN) comprised the bulk of nitrogen leaching from the control except a short period following autumn litterfall. The dominance of DIN was strengthened by litter exclusion, whereas the addition of glucose or fresh litter led to a small increase in dissolved organic nitrogen (DON) and either a temporary or gradual reduction in NO3- release, respectively. Changes in soluble organic C and microbial C in the forest floor implied that increased availability of C sources might have enhanced microbial immobilization of DIN, either temporarily following glucose application or over the longer term following litter addition. The results suggest that detrital quantity and quality can play a crucial role in determining the balance between DIN and DON in N-enriched forest soils. - Detrital quantity and quality play a crucial role in regulating the release of DON and DIN from the forest floor under chronic N deposition

  2. The neural crest and neural crest cells: discovery and significance for theories of embryonic organization

    Indian Academy of Sciences (India)

    Brian K Hall

    2008-12-01

    The neural crest has long fascinated developmental biologists, and, increasingly over the past decades, evolutionary and evolutionary developmental biologists. The neural crest is the name given to the fold of ectoderm at the junction between neural and epidermal ectoderm in neurula-stage vertebrate embryos. In this sense, the neural crest is a morphological term akin to head fold or limb bud. This region of the dorsal neural tube consists of neural crest cells, a special population(s) of cell, that give rise to an astonishing number of cell types and to an equally astonishing number of tissues and organs. Neural crest cell contributions may be direct — providing cells — or indirect — providing a necessary, often inductive, environment in which other cells develop. The enormous range of cell types produced provides an important source of evidence of the neural crest as a germ layer, bringing the number of germ layers to four — ectoderm, endoderm, mesoderm, and neural crest. In this paper I provide a brief overview of the major phases of investigation into the neural crest and the major players involved, discuss how the origin of the neural crest relates to the origin of the nervous system in vertebrate embryos, discuss the impact on the germ-layer theory of the discovery of the neural crest and of secondary neurulation, and present evidence of the neural crest as the fourth germ layer. A companion paper (Hall, Evol. Biol. 2008) deals with the evolutionary origins of the neural crest and neural crest cells.

  3. On the metal tolerance and resilience capacity of Helichrysum italicum G. Don growing on mine soils

    Science.gov (United States)

    Maleci, Laura; Tani, Corrado; Bini, Claudio; Wahsha, Mohammad

    2016-04-01

    Heavy metal accumulation produces significant physiological and biochemical responses in vascular plants. Plants growing on abandoned mine sites are of particular interest, since they are genetically tolerant to high metal concentrations. In this work we examined the effects of heavy metals (HM) on the morphology of Helichrysum italicum growing on mine soils, with the following objectives: to determine the fate of HM within the soil-plant system; to highlight morphological modifications at anatomical and cytological level; to ascertain the plant tolerance to heavy metals, and their resilience capacity. Wild specimens of Helichrysum italicum, with their soil clod, were gathered from sites with different contamination levels by heavy metals (Cd, Cu, Fe, Pb, Zn) in the abandoned Niccioleta mine (Tuscany, Italy). Plants were brought to the botanical laboratory of the University of Florence, and appeared macroscopically not affected by toxic signals (e.g. reduced growth, leaf necrosis) induced by soil HM concentration. Leaves and roots taken at the same growing season were observed by light microscopy (LM) and transmission electron microscopy (TEM). Light microscopy observations show a clear difference in the cell organization of not-contaminated and contaminated samples. In particular, the secreting trichomes, which are responsible for the characteristic flavour of the plant, present a different morphology in the polluted specimens with respect to the not-polluted ones. Indeed, the latter present the typical trichomes of the Asteraceae family, with two lines of cells bearing the secretion accumulated on the apical cuticular space. Trichomes of the polluted plants, instead, present a completely different morphology, with a stalk of 3-4 cells and a large secreting apical cell (i.e. they are capitate hairs). Samples from contaminated sites, moreover, present a palisade parenchyma less organized, and a reduction of leaf thickness proportional to HM concentration. The poor

  4. Materials for stem cell factories of the future

    Science.gov (United States)

    Celiz, Adam D.; Smith, James G. W.; Langer, Robert; Anderson, Daniel G.; Winkler, David A.; Barrett, David A.; Davies, Martyn C.; Young, Lorraine E.; Denning, Chris; Alexander, Morgan R.

    2014-06-01

    Polymeric substrates are being identified that could permit translation of human pluripotent stem cells from laboratory-based research to industrial-scale biomedicine. Well-defined materials are required to allow cell banking and to provide the raw material for reproducible differentiation into lineages for large-scale drug-screening programs and clinical use. Yet more than 1 billion cells for each patient are needed to replace losses during heart attack, multiple sclerosis and diabetes. Producing this number of cells is challenging, and a rethink of the current predominant cell-derived substrates is needed to provide technology that can be scaled to meet the needs of millions of patients a year. In this Review, we consider the role of materials discovery, an emerging area of materials chemistry that is in large part driven by the challenges posed by biologists to materials scientists.

  5. Evolutionary cell biology: functional insight from "endless forms most beautiful".

    Science.gov (United States)

    Richardson, Elisabeth; Zerr, Kelly; Tsaousis, Anastasios; Dorrell, Richard G; Dacks, Joel B

    2015-12-15

    In animal and fungal model organisms, the complexities of cell biology have been analyzed in exquisite detail and much is known about how these organisms function at the cellular level. However, the model organisms cell biologists generally use include only a tiny fraction of the true diversity of eukaryotic cellular forms. The divergent cellular processes observed in these more distant lineages are still largely unknown in the general scientific community. Despite the relative obscurity of these organisms, comparative studies of them across eukaryotic diversity have had profound implications for our understanding of fundamental cell biology in all species and have revealed the evolution and origins of previously observed cellular processes. In this Perspective, we will discuss the complexity of cell biology found across the eukaryotic tree, and three specific examples of where studies of divergent cell biology have altered our understanding of key functional aspects of mitochondria, plastids, and membrane trafficking. PMID:26668171

  6. On tensegrity in cell mechanics.

    Science.gov (United States)

    Volokh, K Y

    2011-09-01

    All models are wrong, but some are useful. This famous saying mirrors the situation in cell mechanics as well. It looks like no particular model of the cell deformability can be unconditionally preferred over others and different models reveal different aspects of the mechanical behavior of living cells. The purpose of the present work is to discuss the so-called tensegrity models of the cell cytoskeleton. It seems that the role of the cytoskeleton in the overall mechanical response of the cell was not appreciated until Donald Ingber put a strong emphasis on it. It was fortunate that Ingber linked the cytoskeletal structure to the fascinating art of tensegrity architecture. This link sparked interest and argument among biologists, physicists, mathematicians, and engineers. At some point the enthusiasm regarding tensegrity perhaps became overwhelming and as a reaction to that some skepticism built up. To demystify Ingber's ideas the present work aims at pinpointing the meaning of tensegrity and its role in our understanding of the importance of the cytoskeleton for the cell deformability and motility. It should be noted also that this paper emphasizes basic ideas rather than carefully follows the chronology of the development of tensegrity models. The latter can be found in the comprehensive review by Dimitrije Stamenovic (2006) to which the present work is complementary. PMID:21977516

  7. Clones identification of Sequoia sempervirens (D.Don) Endl.in Chile by using PCR-RAPDs technique

    Institute of Scientific and Technical Information of China (English)

    Manuel TORAL IBA(N)EZ; Margarita CARU; Miguel A.HERRERA; Luis GONZALEZ; Luis M.MARTIN; Jorge MIRANDA; Rafael M.NAVARRO-CERRILLO

    2009-01-01

    A protocol of polymerase chain reaction-random amplified polymorphic DNAs (PCR-RAPDs) was established to analyse the gene diversity and genotype identification for clones of Sequoia sempervirens (D.Don) Endl.in Chile.Ten (out of 34) clones from introduction trial located in Voipir-Viilarrica,Chile,were studied.The PCR-RAPDs technique and a modified hexadecyltrimethylammonium bromide (CTAB) protocol were used for genomic DNA extraction.The PCR tests were carried out employing 10-mer random primers.The amplification products were detected by electrophoresis in agarose gels.Forty nine polymorphic bands were obtained with the selected primers (BG04,BF07,BF 12,BF13,and BF 14) and were ordered according to their molecular size.The genetic similarity between samples was calculated by the Jaccard index and a dendrogram was constructed using a cluster analysis of unweighted pair group method using arithmetic averages (UPGMA).Of the primers tested,5 (out of 60) RAPD primers were selected for their reproducibility and high polymorphism.A total of 49 polymorphic RAPD bands were detected out of 252 bands.The genetic similarity analysis demonstrates an extensive genetic variability between the tested clones and the dendrogram depicts the genetic relationships among the clones,suggesting a geographic relationship.The results indicate that the RAPD markers permitted the identification of the assayed clones,although they are derived from the same geographic origin.

  8. Stokes IQUV mapping of α2 CVn & other Ap stars using ESPaDOnS and NARVAL

    Science.gov (United States)

    Silvester, J.; Kochukhov, O.; Wade, G. A.

    2013-12-01

    New spectral line polarisation observations of 7 bright Ap stars have been obtained with the ESPaDOnS and Narval high resolution spectropolarimeters (Silvester et al. 2012). The aim of this data set is produce a series of surface magnetic field and surface chemistry maps for these Ap stars. We present new magnetic maps for the Ap star α2 CVn using these new data and the MDI inversion code INVERS10. α2 CVn is the first Ap star to be observed during two separate epochs using high resolution phase resolved spectropolarimetric IQUV observations and as such allows us an insight into how stable the surface magnetic structure is over a decade timescale. We show that the new maps give a magnetic field structure consistent with the previous maps obtained by Kochukhov and Wade (2010) from lower quality MuSiCoS spectra taken a decade ago and that the field topology cannot be described by a dipolar or quadrupolar field.

  9. Stokes IQUV mapping of $\\alpha^2$ CVn & other Ap stars using ESPaDOnS and NARVAL

    CERN Document Server

    Silvester, J; Wade, G A

    2013-01-01

    New spectral line polarisation observations of 7 bright Ap stars have been obtained with the ESPaDOnS and Narval high resolution spectropolarimeters (Silvester et al. 2012). The aim of this data set is produce a series of surface magnetic field and surface chemistry maps for these Ap stars. We present new magnetic maps for the Ap star $\\alpha^2$ CVn using these new data and the MDI inversion code INVERS10. $\\alpha^2$ CVn is the first Ap star to be observed during two separate epochs using high resolution phase resolved spectropolarimetric $IQUV$ observations and as such allows us an insight into how stable the surface magnetic structure is over a decade timescale. We show that the new maps give a magnetic field structure consistent with the previous maps obtained by Kochukhov and Wade (2010) from lower quality MuSiCoS spectra taken a decade ago and that the field topology cannot be described by a dipolar or quadrupolar field.

  10. Modelling the impact of riverine DON removal by marine bacterioplankton on primary production in the Arctic Ocean

    Science.gov (United States)

    Le Fouest, V.; Manizza, M.; Tremblay, B.; Babin, M.

    2015-06-01

    The planktonic and biogeochemical dynamics of the Arctic shelves exhibit a strong variability in response to Arctic warming. In this study, we employ a biogeochemical model coupled to a pan-Arctic ocean-sea ice model (MITgcm) to elucidate the processes regulating the primary production (PP) of phytoplankton, bacterioplankton (BP), and their interactions. The model explicitly simulates and quantifies the contribution of usable dissolved organic nitrogen (DON) drained by the major circum-Arctic rivers to PP and BP in a scenario of melting sea ice (1998-2011). Model simulations suggest that, on average between 1998 and 2011, the removal of usable riverine dissolved organic nitrogen (RDON) by bacterioplankton is responsible for a ~ 26% increase in the annual BP for the whole Arctic Ocean. With respect to total PP, the model simulates an increase of ~ 8% on an annual basis and of ~ 18% in summer. Recycled ammonium is responsible for the PP increase. The recycling of RDON by bacterioplankton promotes higher BP and PP, but there is no significant temporal trend in the BP : PP ratio within the ice-free shelves over the 1998-2011 period. This suggests no significant evolution in the balance between autotrophy and heterotrophy in the last decade, with a constant annual flux of RDON into the coastal ocean, although changes in RDON supply and further reduction in sea-ice cover could potentially alter this delicate balance.

  11. Modeling the impact of riverine DON removal by marine bacterioplankton on primary production in the Arctic Ocean

    Science.gov (United States)

    Le Fouest, V.; Manizza, M.; Tremblay, B.; Babin, M.

    2014-12-01

    The planktonic and biogeochemical dynamics of the Arctic shelves exhibit a strong variability in response to Arctic warming. In this study, in order to elucidate on the processes regulating the production of phytoplankton (PP) and bacterioplankton (BP) and their interactions, we employ a biogeochemical model coupled to a pan-Arctic ocean-sea ice model (MITgcm) to explicitly simulate and quantify the contribution of usable dissolved organic nitrogen (DON) drained by the major circum-Arctic rivers on PP and BP in a scenario of melting sea ice (1998-2011). Model simulations suggest that on average between 1998 and 2011, the removal of usable RDON by bacterioplankton is responsible of a ~26% increase of the annual BP for the whole Arctic Ocean. With respect to total PP, the model simulates an increase of ~8% on an annual basis and of ~18% in summer. Recycled ammonium is responsible for the PP increase. The recycling of RDON by bacterioplankton promotes higher BP and PP but there is no significant temporal trend in the BP : PP ratio within the ice-free shelves over the 1998-2011 period. This suggests no significant evolution in the balance between autotrophy and heterotrophy in the last decade with a constant annual flux of RDON into the coastal ocean although changes in RDON supply and further reduction in sea ice cover could potentially alter this delicate balance.

  12. Relationship among soil parameters, tree nutrition and site index of Pinus radiata D. Don in Asturias, NW Spain

    Energy Technology Data Exchange (ETDEWEB)

    Afif-Khouri, E.; Camara Obregon, M. A.; Oliveira-Prendes, J. A.; Gorgoso-Varela, J. J.; Canga-Libano, E.

    2010-07-01

    The relationships among soil parameters, tree nutrition and site index were examined in Pinus radiata D. Don stands in a climatically homogeneous area of NW Spain. Thirty-eight even-aged stands, ranging from 10 to 54 years, were sampled. In each stand, a representative plot of 0.1 ha was selected and different stand variables and parent material were considered. The soils in the study area are strongly acidic, with high proportions of organic matter, high C/N ratios, and low exchangeable base cation and available P concentration extracted by Mehlich 3 method (PM3). Although foliar N was sufficient in every stand studied, widespread deficiencies of K, P and, to a lesser extent, Mg and Ca were diagnosed. The foliar concentrations of P were positively correlated with PM3 and effective cation exchange capacity. The SI values ranged between 9.5 and 28.8 m and were positively correlated with foliar P and extractable K in soil. In the stands developed on quartzite and sandstone lithologies, the SI was negatively correlated with slope and foliar N respectively. The results suggest the importance of site selection and fertilizer treatment in reforestation programmes. (Author) 63 refs.

  13. ’Freud is finished, Einstein’s next’: Don DeLillo’s Cosmopolis, Chaos Theory, and Quantum Entanglement

    Directory of Open Access Journals (Sweden)

    Crystal Alberts

    2016-05-01

    Full Text Available This essay argues that, in 'Cosmopolis', DeLillo returns to mathematical topics and formal structures, like those presented in Ratner's Star, and expands them to reflect more recent developments in science and technology to contemplate time—the subject that permeates many of his twenty first century novels—and to highlight, variously, the unpredictable, uncertain, interconnected, and illusory nature of the contemporary world. Written in between the dot-com bubble burst and the aftermath of September 11, 2001, this essay asserts that 'Cosmopolis' is liminal: it offers a glimpse of the ruins of the future, and as one of its rejected titles suggests, it approaches an omega point. Drawing heavily on archival research in the 'Don DeLillo Papers' at University of Texas, Austin's Harry Ransom Center, this essay contends that 'Cosmopolis' is a thought experiment, of sorts, in which DeLillo incorporates his substantial and ongoing research on strange attractors, quantum entanglement, and the physics of time to consider the "[t]wo forces in this world, past and future." In doing so, this essay suggests that DeLillo calls for a reassessment of the time in which this text is set and proposes an alternate way of perceiving the post-9/11 world.

  14. Hippophae salicifolia D.Don berries attenuate cerebral ischemia reperfusion injury in a rat model of middle cerebral artery occlusion

    Institute of Scientific and Technical Information of China (English)

    Santhrani Thakur; Pradeepthi Chilikuri; Bindu Pulugurtha; Lavanya Yaidikar

    2015-01-01

    Objective: To investigate the protective effect of Hippophae salicifolia D.Don (H. salicifolia) berries extract against cerebral reperfusion injury induced neurobehavioral and neurochemical changes in a rat model of middle cerebral artery occlusion (MCAO). Methods: Rats were pretreated with alcoholic extract of H. salicifolia (250 and 500 mg/kg) for 14 d and focal cerebral ischemia was induced by MCAO. After 60 min of MCAO, reperfused for 24 h, a battery of behavioral tests were assessed the extent of neurological deficits. Infarct volume and brain edema were measured in 2,3,5-triphenyltetrazolium chloride stained brain sections. TNF-α, oxidative stress parameters like reduced glutathione, calcium, glutamate, malondialdehyde and apoptotic parameters like caspase-3, and caspase-9 were estimated in the brain homogenates. Results:Pretreatment with alcoholic extract of H. salicifolia at doses of 250 and 500 mg/kg significantly improved the neurobehavioral alterations and reduced the infarct volume, edema induced by ischemia reperfusion injury. H. salicifolia significantly prevented ischemia induced increase in malondialdehyde, glutamate, calcium, caspase-3, caspase-9 and TNF-αlevels as compared to ischemic animals. Conclusions: Our results indicate that H. salicifolia mitigated the ischemia reperfusion induced neuronal damage.

  15. Repellent Activity of the Essential Oil from the Heartwood of Pilgerodendron uviferum (D. Don) Florin against Aegorhinus superciliosus (Coleoptera: Curculionidae).

    Science.gov (United States)

    Espinoza, Javier; Urzúa, Alejandro; Tampe, Jocelyne; Parra, Leonardo; Quiroz, Andrés

    2016-01-01

    The weevil Aegorhinus superciliosus Guérin (Coleoptera: Curculionidae), which is endemic to Central-Southern Chile and Argentina, is one of the major berry pests in Chile and the most important pest in the La Araucanía Region (38°44'9″S, 72°35'25″W). Due to the poor effectiveness and problems surrounding the implementation of the traditional control methods using organophosphate and carbamate insecticides, new strategies for controlling this pest are needed. In this communication, we evaluated the behavioral responses of male and female A. superciliosus to volatile compounds released from the essential oil (EO) obtained from the heartwood of Pilgerodendron uviferum (D. Don) Florin using olfactometric bioassays. The composition of the EO was analyzed using gas chromatography (GC) and gas chromatography/mass spectrometry (GC/MS). According to these analyses, δ-cadinol (24.16%), cubenol (22.64%), 15-copaenol (15.46%) and δ-cadinene (10.81%) were the principal components of the EO. The Pilgerodendron uviferum EO, which is almost exclusively composed of sesquiterpenes (99.5%), exhibited a repellent effect against A. superciliosus adults, regardless of the sex or concentration used (56.6 mg/cm³ and 1.58 × 10(-2) mg/cm³). The EO has low volatility and greater persistence than the EOs composed of monoterpenes and is considered a good model in the search for raspberry weevil repellents. PMID:27110756

  16. Synthetic biology outside the cell: linking computational tools to cell-free systems.

    Science.gov (United States)

    Lewis, Daniel D; Villarreal, Fernando D; Wu, Fan; Tan, Cheemeng

    2014-01-01

    As mathematical models become more commonly integrated into the study of biology, a common language for describing biological processes is manifesting. Many tools have emerged for the simulation of in vivo synthetic biological systems, with only a few examples of prominent work done on predicting the dynamics of cell-free synthetic systems. At the same time, experimental biologists have begun to study dynamics of in vitro systems encapsulated by amphiphilic molecules, opening the door for the development of a new generation of biomimetic systems. In this review, we explore both in vivo and in vitro models of biochemical networks with a special focus on tools that could be applied to the construction of cell-free expression systems. We believe that quantitative studies of complex cellular mechanisms and pathways in synthetic systems can yield important insights into what makes cells different from conventional chemical systems. PMID:25538941

  17. Synthetic Biology Outside the Cell: Linking Computational Tools to Cell-Free Systems

    Directory of Open Access Journals (Sweden)

    Daniel eLewis

    2014-12-01

    Full Text Available As mathematical models become more commonly integrated into the study of biology, a common language for describing biological processes is manifesting. Many tools have emerged for the simulation of in vivo systems, with only a few examples of prominent work done on predicting the dynamics of cell-free systems. At the same time, experimental biologists have begun to study dynamics of in vitro systems encapsulated by amphiphilic molecules, opening the door for the development of a new generation of biomimetic systems. In this review, we explore both in vivo and in vitro models of biochemical networks with a special focus on tools that could be applied to the construction of cell-free expression systems. We believe that quantitative studies of complex cellular mechanisms and pathways in synthetic systems can yield important insights into what makes cells different from conventional chemical systems.

  18. Low cholesterol? Don't brag yet ... hypocholesterolemia blunts HAART effectiveness: a longitudinal study

    Directory of Open Access Journals (Sweden)

    Míguez María

    2010-07-01

    Full Text Available Abstract Background In vitro studies suggest that reducing cholesterol inhibits HIV replication. However, this effect may not hold in vivo, where other factors, such as cholesterol's immunomodulatory properties, may interact. Methods Fasting blood samples were obtained on 165 people living with HIV at baseline and after 24 weeks on highly active antiretroviral therapy (HAART. Participants were classified as hypocholesterolemic (HypoCHL; 150 mg/dl and were compared on viro-immune outcomes. Results At baseline, participants with HypoCHL (40% exhibited lower CD4 (197 ± 181 vs. 295 ± 191 cells/mm3, p = 0.02 and CD8 (823 ± 448 vs. 1194 ± 598 cells/mm3, p = 0.001 counts and were more likely to have detectable viral loads (OR = 3.5, p = 0.01 than non-HypoCHL controls. After HAART, participants with HypoCHL were twice as likely to experience a virological failure >400 copies (95% CI 1-2.6, p = 0.05 and to exhibit Conclusions The study implicates, for the first time, HypoCHL with impaired HAART effectiveness, including limited CD4 repletion by the thymus and suboptimal viral clearance.

  19. Oligomers of heat-shock proteins: Structures that don't imply function

    CERN Document Server

    Jacobs, William M; Frenkel, Daan

    2015-01-01

    Most proteins must remain soluble in the cytosol in order to perform their biological functions. To protect against undesired protein aggregation, living cells maintain a population of molecular chaperones that ensure the solubility of the proteome. Here we report simulations of a lattice model of interacting proteins to understand how low concentrations of passive molecular chaperones, such as small heat-shock proteins, suppress thermodynamic instabilities in protein solutions. Given fixed concentrations of chaperones and client proteins, the solubility of the proteome can be increased by tuning the chaperone--client binding strength. Surprisingly, we find that the binding strength that optimizes solubility while preventing irreversible chaperone binding also promotes the formation of weakly bound chaperone oligomers, although the presence of these oligomers does not significantly affect the thermodynamic stability of the solution. Such oligomers are commonly observed in experiments on small heat-shock prote...

  20. Estimation of camptothecin and pharmacological evaluation of Ophiorrhiza prostrata D. Don and Ophiorrhiza mungos L.

    Institute of Scientific and Technical Information of China (English)

    Krishnakumar G; Rameshkumar KB; Priya Srinivas; Satheeshkumar K; Krishnan PN

    2012-01-01

    Objective: To carry out the qualitative and quantitative evaluation of camptothecin, estimation of total phenolic compounds and evaluation of in vitro antioxidant activity and cytotoxic activity of Ophiorrhiza prostrata and Ophiorrhiza mungos. Methods: Direct Analysis in Real Time- Mass Spectrometry (DART-MS) was employed for the detection of camptothecin in the Ophiorrhiza species, while high performance thin layer chromatography (HPTLC) was used for the estimation of camptothecin. Total phenolic compounds were estimated by modified Folins-Ciocalteu’s reagent method. Antioxidant activity was evaluated through DPPH radical, hydroxyl radical, superoxide radical scavenging assays and reducing power assay. The cytotoxicity evaluation was performed using MTT assay on MCF-7 cell lines. Results: The presence of camptothecin was confirmed in both the species by the [M++H] peak at 349 by DART-MS analysis. Camptothecin content was estimated as 1.47 μg/gm (dry wt) in O. prostrata and 188.60 μg/gm (dry wt) in O.mungos using HPTLC method. The moderate in vitro antioxidant activities of the methanol extracts corroborates with the low content of phenolic compounds in O. prostrata (9.88 GAE mg/g) and O. mungos (12.73 GAE mg/g). The methanol extract of O. prostrata exhibited remarkable cytotoxicity on human breast cancer cell lines (MCF-7), with IC50 value 1.10μg/mL compared to O. mungos (3.48μg/mL) and standard camptothecin (3.51μg/mL). Conclusions: The application of DART-MS proved to be a simple and rapid technique for the detection of camptothecin in Ophiorrhiza species. The higher cytotoxicity for O. prostrata, despite the low content of camptothecin suggests the presence of other potential cytotoxic compounds in O. prostrata.

  1. Petrografía y geoquímica de las rocas gondwánicas del proyecto minero Don Sixto, Mendoza Petrography and geochemistry of the gondwanic rock units from Don Sixto mining project, Mendoza

    Directory of Open Access Journals (Sweden)

    Ana Cecilia Mugas Lobos

    2010-11-01

    Full Text Available El proyecto minero Don Sixto es un depósito epitermal de Au-Ag de baja sulfuración ubicado al sureste de la provincia de Mendoza; el recurso aurífero de este depósito es próximo a las 900.000 onzas. La mineralización en el área se encuentra principalmente diseminada y está alojada mayormente en volcanitas y piroclastitas riolíticas de la Formación Choique Mahuida y diques riolíticos del Grupo El Portillo, ambos representantes de la sección superior del Grupo Choiyoi. Los datos obtenidos por el relevamiento geológico y los estudios petrográficos realizados en las volcanitas-piroclastitas del área de estudio, permiten indicar que la Formación Choique Mahuida está representada por riolitas, intercaladas con mantos ignimbríticos y niveles aislados de depósitos lenticulares piroclásticos. Por su parte, los diques riolíticos del Grupo El Portillo, de rumbo general noroeste-sureste y norte-sur, están probablemente vinculados a la evolución de zonas de falla de carácter regional. La geoquímica de estas riolitas gondwánicas, de acuerdo a la distribución de sus elementos traza y tierras raras, indica que están genéticamente vinculadas entre sí y pertenecerían a una suite cosanguínea proveniente de magmas diferenciados más evolucionados que los conocidos hasta la fecha para rocas de la sección superior del Grupo Choiyoi. Las características geoquímicas de estos magmas concuerdan además con los datos conocidos para rocas equivalentes fuera del área de estudio, en base a los cuales se asigna la sección superior del Grupo a un ambiente geotectónico transicional entre uno de arco volcánico y uno de intraplaca.Don Sixto mining project is a low sulphidation epithermal Au-Ag ore deposit located at the southeast of Mendoza province; the gold resource of the deposit is close to 900.000 ounces. In the study area, the mineralization is mainly disseminated and it is hosted by rhyolitic volcanic and pyroclastic sequences of the

  2. Sombreamento de plantas de Catharanthus roseus (L. G. Don 'Pacifica White' por malhas coloridas: desenvolvimento vegetativo Shading of 'Pacifica White' Catharanthus roseus (L. G. Don plants with colored nets: vegetative development

    Directory of Open Access Journals (Sweden)

    Anderson Adriano Martins Melo

    2009-04-01

    Full Text Available As malhas coloridas têm sido utilizadas para manipular o desenvolvimento vegetativo, melhorando a utilização da radiação solar por plantas ornamentais. Objetivou-se, neste trabalho estudar o efeito da redução de 50% da radiação fotossinteticamente ativa sobre o crescimento vegetativo de plantas de Catharanthus roseus (L. G. Don, por meio de malhas azul e vermelha e malha preta, em comparação com plantas crescidas na ausência de sombreamento (pleno sol. As plantas foram obtidas a partir de sementes e tratadas por 180 dias. Foram avaliados o ganho de biomassa e a distribuição de matéria seca nas plantas, o conteúdo de pigmentos foliares (clorofilas e carotenóides e de nitrogênio foliar. A malha vermelha provocou um aumento de matéria seca total e de área foliar das plantas em comparação com as malhas azul, preta e ao tratamento a pleno sol, porém, exceto em relação a esse tratamento, a malha vermelha causou menor conteúdo de nitrogênio e pigmentos foliares. A maior relação raiz/parte aérea e relação clorofila a/b, menores razões de área foliar e de massa foliar das plantas crescidas a pleno sol em relação às plantas sombreadas indicam um efeito mais proeminente da irradiância mais alta do que da alteração do espectro de luz. O sombreamento altera significativamente a distribuição de matéria seca e o uso de malhas de diferentes cores modifica o conteúdo de pigmentos fotossintéticos dessa espécie.Colored shade nets have been used to manipulate the vegetative development, improving the utilization of solar radiation by ornamental plants. This work aimed to study the effect of 50% reduction of PAR on vegetative growth of plants of Catharanthus roseus (L. G. Don, using blue and red nets, and black net, in comparison to plants growing under full sunlight (lack of shading. The plants were obtained from seeds and treated for 180 days. Biomass increment and distribution, pigment content (chlorophylls and

  3. Polimorfismo do gene tp53 no códon 72 em pacientes com suspeita de LMC Codon 72 polymorphism of the TP53 gene in patients suspected to have CML

    Directory of Open Access Journals (Sweden)

    Camila S. Hamú

    2007-12-01

    Full Text Available A leucemia mielóide crônica (LMC é uma doença proliferativa do sistema hematopoiético, caracterizada pela expansão clonal de uma célula-tronco primitiva e pluripotente denominada stem cell. Este tipo de leucemia está associado, em 90% dos casos, à translocação t(9;22(q34;q11. Essa alteração cromossômica estrutural codifica para uma proteína quimérica BCR-ABL, que confere às células leucêmicas uma alta resistência à morte, independente do agente indutor desse processo. A proteína p53 é uma reguladora transcricional induzida por danos no DNA, fato que resulta na parada do ciclo celular com conseqüente ativação de mecanismos de reparo ou mesmo na indução à apoptose. As mutações no gene TP53 são as alterações genéticas mais comuns em tumores malignos humanos. O presente estudo teve como objetivo genotipar e determinar a freqüência alélica do polimorfismo do TP53 no códon 72 (arginina - Arg e prolina - Pro, em pacientes com suspeita de LMC, pela Reação em Cadeia da Polimerase. Desta forma, os resultados indicaram que 73,4% (23/30 dos pacientes apresentaram homozigose para arginina (Arg/Arg e 26,6% (7/30 heterozigose (Arg/Pro. Não foi encontrado nenhum paciente homozigoto para prolina (Pro/Pro. Os resultados obtidos sugerem que o polimorfismo do gene TP53 no códon 72 não é um fator de risco importante para a iniciação, promoção e progressão da LMC.Chronic myeloid leukemia (CML is a proliferative disorder of the hematopoietic system characterized by clonal expansion of a primitive and pluripotent stem cell. In this type of leukemia, up to 90% of all cases is associated to a specific chromosomal translocation, t(9;22(q34;q11. The genomic alteration results in a chimeric protein, BCR-ABL, that confers a high resistance leukemia cells to death, independent of the induction mechanism of this process. Protein p53 is a transcriptional factor expressed after DNA damage which ceases cell cycle progression and

  4. The Do's and Don'ts of Neurofeedback Training: A Review of the Controlled Studies Using Healthy Adults.

    Science.gov (United States)

    Rogala, Jacek; Jurewicz, Katarzyna; Paluch, Katarzyna; Kublik, Ewa; Cetnarski, Ryszard; Wróbel, Andrzej

    2016-01-01

    The goal of EEG neurofeedback (EEG-NFB) training is to induce changes in the power of targeted EEG bands to produce beneficial changes in cognitive or motor function. The effectiveness of different EEG-NFB protocols can be measured using two dependent variables: (1) changes in EEG activity and (2) behavioral changes of a targeted function (for therapeutic applications the desired changes should be long-lasting). To firmly establish a causal link between these variables and the selected protocol, similar changes should not be observed when appropriate control paradigms are used. The main objective of this review is to evaluate the evidence, reported in the scientific literature, which supports the validity of various EEG-NFB protocols. Our primary concern is to highlight the role that uncontrolled nonspecific factors can play in the results generated from EEG-NFB studies. Nonspecific factors are often ignored in EEG-NFB designs or the data are not presented, which means conclusions should be interpreted cautiously. As an outcome of this review we present a do's and don'ts list, which can be used to develop future EEG-NFB methodologies, based on the small set of experiments in which the proper control groups have excluded non-EEG-NFB related effects. We found two features which positively correlated with the expected changes in power of the trained EEG band(s): (1) protocols which focused on training a smaller number of frequency bands and (2) a bigger number of electrodes used for neurofeedback training. However, we did not find evidence in support of the positive relationship between power changes of a trained frequency band(s) and specific behavioral effects. PMID:27378892

  5. The possibility of crop cultivation and utilization of edible gum from herb (Dorema ammoniacum D. Don in dryland farming

    Directory of Open Access Journals (Sweden)

    Gholami Barat Ali

    2015-01-01

    Full Text Available Gum ammoniacum (Dorema ammoniacum D. Don is one of the most important food, industrial and medicinal plants of Iran which is mainly distributed in semi-arid and desert areas. It is endangered due to superfluous and unsustainable harvesting methods. The objective of this study was to evaluate the possibility of crop cultivation in terms of dryland farming and the best operation method for collecting gum resin. For this purpose, the effects of different scarification methods (traditional, concave and staircase and cut-off frequencies (5, 10 and 13 times on gum yield of the five-year-old plants were studied at Toroq farm in Mashhad. The treatments were studied under a split plot structure in time experiment which was arranged in a completely randomized design with three replications. The rate of the root gum production and the rate of plant survival in the years after the withdrawal were measured, and finally the data were analyzed. Results showed no significant differences among cutting methods at p<0.05. The yield of gum was affected by times of harvesting and the highest yield was observed (p<0.01 in 13 cut-off times with 31.67 g/plant. The lowest gum yields were seen in 5 cut-off times with 5.84 g/plant. The traditional cutting method destroyed crown buds and caused the death of the plant (p<0.01. Reasonable gum yields and plant regeneration rates were obtained with the use of stairs method which is an easy method to be applied by the farmers or beneficiaries.

  6. Evaluation of antipyretic activity of ethyl acetate extract of Adenema hyssopifolium G. Don in a rat model

    Institute of Scientific and Technical Information of China (English)

    Aiyalu Rajasekaran; Ramasamy Arivukkarasu; Shanmugasundaram Murugesh

    2010-01-01

    Objective:To evaluate the effect of ethyl acetate extract of Adenema hyssopifolium (AHEAE) on normal body temperature and brewer’s yeast-induced pyrexia rats. Methods:Preliminary phytochemical tests, acute toxicity tests and antipyretic evaluation were carried out in ethyl acetate extract of Adenema hyssopifolium. Two doses of the extract (300 or 600 mg/kg orally) and standard antipyretic agent, paracetamol at a dose of 150 mg/kg were administered to various group of the rats. Mean rectal temperature before and after treatment was noted. Results:The phytochemical analysis of AHEAE revealed the presence of flavonoid and iridoid glycosides as major phytoconstituents. The administration of AHEAE at a dose of 300 or 600 mg/kg produced significant reduction (P<0.001 and P<0.01) of the body temperature in normal and pyrexia rats on a dose dependent manner. The antipyretic influence of AHEAE was comparable to that of standard antipyretic agent, paracetamol (150 mg/kg), and onset of action and reduction in pyrexia towards normal body temperature was delayed when compared to paracetamol treatment. At dose of 600 mg/kg, AHEAE reduced pyrexia to normal body temperature at 4 h after its administration compared to reduction of pyrexia to normal body temperature at 2 h by standard drug. The reduction of fever was consistent in paracetamol group from 2 to 4 h after its administration to normal body temperature compared to AHEAE treatments. Conclusions:Our present results corroborate with the traditional notion of Adenema hyssopifolium G. DON that is being used as an effective cure of fever and add authenticity to claim of indigenous healers that the taxon is a potential antipyretic agent.

  7. M2-F1 on lakebed with pilots Milt Thompson, Chuck Yeager, Don Mallick, and Bruce Peterson

    Science.gov (United States)

    1963-01-01

    After the initial M2-F1 airtow flights, the NASA Flight Research Center used the vehicle to check out other pilots. Bruce Peterson was scheduled to take over as the M2-F1 project pilot from Milt Thompson, while Don Mallick was to be his backup. Col. (later Brig. Gen.) Charles (Chuck) Yeager, then commandant of the Air Force's Aerospace Research Pilots School, wanted to evaluate a possible lifting-body trainer for the school. This photo shows all of these distinguished pilots on or in the M2-F1, with Col. Yeager in the pilot's seat. The lifting body concept evolved in the mid-1950s as researchers considered alternatives to ballistic reentries of piloted space capsules. The designs for hypersonic, wingless vehicles were on the boards at NASA Ames and NASA Langley facilities, while the US Air Force was gearing up for its Dyna-Soar program, which defined the need for a spacecraft that would land like an airplane. Despite favorable research on lifting bodies, there was little support for a flight program. Dryden engineer R. Dale Reed was intrigued with the lifting body concept, and reasoned that some sort of flight demonstration was needed before wingless aircraft could be taken seriously. In February 1962, he built a model lifting body based upon the Ames M2 design, and air-launched it from a radio controlled 'mothership.' Home movies of these flights, plus the support of research pilot Milt Thompson, helped pursuade the facilities director, Paul Bikle, to give the go-ahead for the construction of a full-scale version, to be used as a wind-tunnel model and possibly flown as a glider. Comparing lifting bodies to space capsules, an unofficial motto of the project was, 'Don't be Rescued from Outer Space--Fly Back in Style.' The construction of the M2-F1 was a joint effort by Dryden and a local glider manufacturer, the Briegleb Glider Company. The budget was $30,000. NASA craftsmen and engineers built the tubular steel interior frame. Its mahogany plywood shell was hand

  8. Heavy metal accumulation in Pyrrosia flocculosa (D. Don) Ching growing in sites located along a vehicular disturbance gradient.

    Science.gov (United States)

    Sharma, Alpy; Uniyal, Sanjay Kr

    2015-10-01

    Monitoring of environment is a key contemporary issue that has necessitated search for bio-indicators. The very fact that epiphytes do not have a direct contact with soil and absorb nutrients from the environment puts them among the best indicators of environmental conditions. We, therefore, selected Pyrrosia flocculosa (D. Don) Ching-an epiphytic fern that commonly occurs in the Himalaya for this study. The study focused on analyzing heavy metal concentrations in the fronds of P. flocculosa growing along a disturbance gradient. For this, three sites representing different levels of disturbance viz., least disturbed, moderately disturbed, and highly disturbed, were identified in Kangra district of Himachal Pradesh. From each site, fronds of P. flocculosa were collected, categorized into three growth stages (juvenile, young, and mature), and brought to the laboratory for analyses. After drying and powdering, the samples were analyzed for Pb, Cd, Fe, Ni, Cu, Mn, and Zn using atomic absorption spectrophotometer. The results obtained were statistically compared using the software package Statistica. As expected, concentration of the metals varied among the sites and also among the identified growth stages of the species. In general, concentration of the metals was in the order Fe (639.28 ± 81.63) > Ni (56.03 ± 4.97) > Mn (7.54 ± 0.69) > Zn (6.51 ± 0.36) > Cd (4.01 ± 0.86) > Cu (1.93 ± 0.74). Barring Mn, concentration of all the metals increased with disturbance and was positively correlated to it. However, except for Cd and Fe, none of the metals reported higher than threshold values. Effective monitoring of the environment can thus be done using P. flocculosa. PMID:27591984

  9. La categoría de don en el marco de la economía social y solidaria

    Directory of Open Access Journals (Sweden)

    Susana Presta

    2007-12-01

    Full Text Available La crisis de los años '90 ha revitalizado los preceptos de la Economía Social propiciando distintos emprendimientos cooperativos. Este artículo analiza el caso de una Cooperativa Educacional y su Banco de Horas desde la perspectiva de la categoría de don , para ahondar no sólo en el análisis de la construcción de relaciones sociales al interior de dicha organización socioeconómica, sino también avanzar sobre las formas en que la misma se vincula con distintas corporaciones transnacionales a partir de diversas donaciones.A crise do '90 criou os preceitos da Economia Social como uma guia a projetos cooperativos diferentes. Neste artigo, analisamos uma Cooperativa Educativa e seu Banco de Horas dentro da perspectiva da categoria da dádiva, para aumentar a nossa compreensão sobre a construção de relações sociais dentro desta organização socioeconômica, mas alem tentaremos um analise das relações entre o Banco de Horas e as corporações transnacionais diferentes que lhe fazem doações.The crisis of the 90 th has brought up the precepts of the Social Economy as a guide to different cooperative projects. In this article, we analyze an Educational Cooperative and its Hours Bank within the perspective of the category of gift in order to increase our comprehension about the social relations construction inside this socioeconomic organization, but also we will try to go further into the relations between the Hours Bank and the different transnational corporations that make donations to them.

  10. Which patients don't need a tumor-bed boost after whole-breast radiotherapy?

    International Nuclear Information System (INIS)

    Background: Retrospective dose-response data suggest that a boost of about 15 Gy to the tumor bed following whole-breast radiotherapy reduces the risk of local recurrence (IBTR) by as much as 2-fold. Even if this benefit is confirmed by prospective trials, boost irradiation may not be considered cost-effective in patients having an IBTR risk of less than 1% per annum. Methods: Published prospective trials of invasive breast cancers (National Surgical Adjuvant Breast and Bowel Project; Stockholm Adjuvant Tamoxifen Trial) in which patients received 50 Gy whole-breast irradiation, without a boost, were analyzed to identify subgroups with IBTR risk less than 1% per year. All studies were based on lumpectomy (rather than segmental or quadrant excision) and eligibility required the absence of cancer cells at the margins. It was assumed that clinical and pathological factors, other than those defining trial eligibility, were of negligible importance regarding IBTR risk, with the exception of young age. Results: All patients not receiving adjuvant systemic therapy, including tumors<1 cm, have IBTR risk justifying boost irradiation. Of patients receiving systemic therapy, only patients with node-negative, estrogen receptor-positive tumors have low IBTR risk (3% at 10 years), provided that tamoxifen is administered. Of patients receiving only adjuvant chemotherapy, low IBTR risk seems to be associated with administration concomitantly with radiotherapy. IBTR risk in patients receiving chemotherapy sequentially with respect to radiotherapy probably is high enough to justify a boost. A boost is probably indicated in all patients younger than 40 years, regardless of other factors. (orig.)

  11. A focus on parietal cells as a renewing cell population

    Directory of Open Access Journals (Sweden)

    Sherif M Karam

    2010-02-01

    Full Text Available The fact that the acid-secreting parietal cells undergo continuous renewal has been ignored by many gastroenterologists and cell biologists. In the past, it was thought that these cells were static. However, by using 3H-thymidine radioautography in combination with electron microscopy, it was possible to demonstrate that parietal cells belong to a continuously renewing epithelial cell lineage. In the gastric glands, stem cells anchored in the isthmus region are responsible for the production of parietal cells. The stem cells give rise to three main progenitors: prepit, preneck and preparietal cells. Parietal cells develop either directly from the non-cycling preparietal cells or less commonly via differentiation of the cycling prepit and preneck cell progenitors. The formation of a parietal cell is a sequential process which involves diminishment of glycocalyx, production of cytoplasmic tubulovesicles, an increase in number and length of microvilli, an increase in number and size of mitochondria, and finally, expansion and invagination of the apical membrane with the formation of an intracellular canalicular system. Little is known about the genetic counterparts of these morphological events. However, the time dimension of parietal cell production and the consequences of its alteration on the biological features of the gastric gland are well documented. The production of a new parietal cell takes about 2 d. However, mature parietal cells have a long lifespan during which they migrate bi-directionally while their functional activity for acid secretion gradually diminishes. Following an average lifespan of about 54 d, in mice, old parietal cells undergo degeneration and elimination. Various approaches for genetic alteration of the development of parietal cells have provided evidence in support of their role as governors of the stem/progenitor cell proliferation and differentiation programs. Revealing the dynamic features and the various roles of

  12. Secondary Analysis of the NCI-60 Whole Exome Sequencing Data Indicates Significant Presence of Propionibacterium acnes Genomic Material in Leukemia (RPMI-8226) and Central Nervous System (SF-295, SF-539, and SNB-19) Cell Lines

    OpenAIRE

    Rojas, Mark; Golovko, Georgiy; Khanipov, Kamil; Albayrak, Levent; Chumakov, Sergei; Pettitt, B. Montgomery; Strongin, Alex Y.; Fofanov, Yuriy

    2015-01-01

    The NCI-60 human tumor cell line panel has been used in a broad range of cancer research over the last two decades. A landmark 2013 whole exome sequencing study of this panel added an exceptional new resource for cancer biologists. The complementary analysis of the sequencing data produced by this study suggests the presence of Propionibacterium acnes genomic sequences in almost half of the datasets, with the highest abundance in the leukemia (RPMI-8226) and central nervous system (SF-295, SF...

  13. The cytotoxic effects a of ACA1 on human melanoma cell line

    OpenAIRE

    R. Yaraie; M.R. Jalali Nadoushan; Naseri, M.; S. Shahrokhi; T. Ghazanfari; Kardar, M.

    2006-01-01

    Background and purpose: Different methods such as surgery, chemotherapy, radiotherapy, hormone therapy and immunotherapy are used for treatment of melanoma cancer. Unfortunately they don't always have desirable results and they may have unfavorable side effects. Researchers try to find new, more effective drugs with low side effects. In this study we evaluated the cytotoxic effect of ACA-1, a water extract of a traditional Iranian medicinal herbs on a melanoma cell line SKMEL-3.Materials and ...

  14. Presagios de muerte de como murió mi padre: relato del abuelo don José García de la nación Muinane

    Directory of Open Access Journals (Sweden)

    Fernando Urbina Rangel

    2010-09-01

    Full Text Available Hace aproximadamente un año, en los comienzos de junio, el Abuelo don José García dio remate a una extensa y rica crónica que cubre tres momentos de su ya larga y fecunda vida. Dos de ellos (a y b tuvieron el mismo escenario: los territorios ancestrales de su tribu en las cabeceras del río Cahuinarí, afluente del Caquetá, pero mediando entre ellos un largo lapso de tiempo de no menos de 35 años. El otro (c ocurre en los parajes, para él vados de historias primordiales, a donde fuera llevado compulsivamente con su tribu por los caucheros peruanos, pocos años antes de la guerra con el Perú. La analogía estructural es muy estrecha entre el primero (a y el que tuvo ocurrencia en un ámbito distinto (c; el más reciente (b es un apéndice temático del primero. El básico (a versa sobre la muerte de Kima Baiji Bocade maguaré, abuelo materno de don José, episodio que remata muchos años después (b cuando don José visita, en compañía de su hijo y su nuera, la tumba del ancestro. Los dos, a y b, me fueron narrados en 1976, en el acogedor hogar del anciano, próximo al quebrado Takana, en las vecindades de Leticia.

  15. Changes in the Subpopulations of Porcine Peripheral Blood Lymphocytes Induced by Exposure to Low Doses of Zearalenone (ZEN and Deoxynivalenol (DON

    Directory of Open Access Journals (Sweden)

    Michał Dąbrowski

    2016-04-01

    Full Text Available Zearalenone and deoxynivalenol are secondary metabolites of fungi of the genus Fusarium. The presence of mycotoxins in cereals and the resulting contamination of feeds and foods pose health risks for animals and humans. The dangers associated with high doses of mycotoxins have been extensively researched but very little is known about NOAEL (No Observed Adverse Effect Level doses or exposure to a combination of mycotoxins (mixed mycotoxicoses. The aim of this study was to determine the effects of six-week exposure to NOAEL doses of individual and combined mycotoxins on the subpopulations of CD4+8−, CD4−8+ and CD4+8+ lymphocytes in the peripheral blood of pigs. The experiment was performed on 72 gilts with average body weight of 25 kg, divided into three experimental groups (E1, E2 and E3, administered zearalenone (ZEN, deoxynivalenol (DON and ZEN + DON, respectively, on a daily basis and a control group (C receiving placebo. Changes in lymphocyte subpopulations were evaluated by flow cytometry at weekly intervals (experimental days 7, 14, 21, 28, 35 and 42. A linear increase in the percentage of CD4+8+ lymphocytes was highly correlated with time (r = 0.682 in group C. The correlations and linear increase in the above subpopulation were disrupted in the remaining groups. In group E3, a statistically significant (p < 0.05 decrease in CD4+8+ counts was observed in week 5, which could point to a transient depletion of regulatory mechanisms of immune responses. The noted results also suggest that in mixed mycotoxicosis, ZEN and DON exerted stronger immunomodulatory effects.

  16. Sperm-cell ultrastructure of North American sturgeons. IV. The pallid sturgeon (Scaphirhynchus albus Forbes and Richardson, 1905)

    Science.gov (United States)

    DiLauro, M.N.; Walsh, R.A.; Peiffer, M.; Bennett, R.M.

    2001-01-01

    Sperm-cell morphology and ultrastructure in the pallid sturgeon (Scaphirhynchus albus) were examined using transmission and scanning electron microscopy. Metrics and structure were compared with similar metrics obtained from other published descriptions of sturgeon sperm cells. General morphology was found to be similar to that of sperm cells of the white (Acipenser transmontanus), lake (A. fulvescens), stellate (A. stellatus), Chinese (A. sinensis), Russian (A. gueldenstaedti colchicus), and shortnose (A. brevirostrum) sturgeons, which all shared a gradual tapering of the nuclear diameter from posterior to anterior, unlike that of the Atlantic sturgeon (A. oxyrhynchus). The sperm cell of the pallid sturgeon was similar in size to that of the Atlantic sturgeon, being only slightly larger. The sperm cell of the pallid sturgeon differed from those of other sturgeons chiefly in the acrosomal region, where the posterolateral projections (PLP) have the shape of an acute triangle and are arranged in a spiral about the longitudinal axis of the cell. The PLP were longer than those of other sturgeons, being twice the length of those of the Atlantic sturgeon and 58% longer than those of the lake sturgeon. Also, in cross section the acrosome had the shape of a hollow cone rather than the cap of an oak tree acorn, as was found in ultrastructural studies of other sturgeons. In addition, we were able to confirm that the structural arrangement of the distal centriole of the midpiece is identical with that of the proximal centriole: nine sets of microtubular triplets around the periphery of the centriole. This information is of potential use to fishery biologists, forensic biologists, zoologists, reproductive physiologists, taxonomists, evolutionary biologists, and aquaculturists.

  17. Respuesta a la sequía de Pinus radiata D. Don y su implicación en los procesos de tolerancia

    OpenAIRE

    De Diego Sánchez, Nuria

    2013-01-01

    160 p. (Bibliogr. 141-160) Correo electrónico de la autora: [ES] En este estudio se evaluaron cinco ecotipos de Pinus radiata D. Don y un híbrido de especie (O4, Pinus radiata x Pinus attenuata) con el fin de caracterizar fisiológicamente su respuesta al estrés hídrico y determinar su tolerancia, capacidad de recuperación y endurecimiento. La plantas de O4 fueron incluidas como modelo de tolerancia debido a la alta resistencia a la sequía descrita en P. attenuata (Begle...

  18. Teoría y práctica del teatro: farsa para guiñol: Retablillo de Don Cristobal

    OpenAIRE

    Santos Tudela, Raúl

    2015-01-01

    Se pretende realizar un análisis de la obra Retablillo de Don Cristobal y poder llevarlo a la práctica dentro del entorno escolar. Para ello primero hemos de centrar dicha obra realizado una biografía de García Lorca, hablando de sus obras teatrales menores, de la frasa, del mundo de los títeres y compararemos el teatro de marionetas con el teatro ordinario. Por último analizaremos la obra en profundidad y diseñaremos una unidad didáctica de ella para poder aplicarla en las aulas

  19. A NEW MODULA TYPO-DIMENSIONAL, CONSTRUCTIVE AND FUNCTIONAL CONCEPT OF VIVA DON EXPERT® FLOATABLE FISH CAGES FOR INTENSIVE AQUACULTURE IN INLAND WATERS

    Directory of Open Access Journals (Sweden)

    D. ONEA

    2013-07-01

    Full Text Available This scientific work presents succinct information about the trials which takes place between 2005-2009 in Constanta (fish farm Canalul Rompetrol. This trials includes the fish farming in cages and leads to finishing off and elaboration of a new modular typo-dimensional, constructive and functional concept of viva don Expert® floatable fish cages for intensive aquaculture in inland waters from Romania like an efficient solution for the qualitative and quantitative increase of local fish production’s (by water volume optimizations, plants, fish farms and technologies optimizations.

  20. Los inicios del programa de artes del Instituto Técnico Obrero Empresarial del Polígono Industrial Don Bosco

    OpenAIRE

    Bernabé Villodre, María del Mar

    2014-01-01

    Este trabajo recoge una experiencia en el Instituto Técnico Obrero Empresarial Don Bosco durante el inicio de la actividad musical de dicha institución y los resultados y el posterior camino que esta actividad inicial supuso. Durante un mes se trabajó con todos los niveles educativos de esta institución para introducirles en el mundo de la música, pero desde una perspectiva específica destinada a potenciar su creatividad como futuros empresarios y a mejorar su formación personal cultural. En ...