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Sample records for cell analysis technical

  1. Technical Analysis of Installed Micro-Combined Heat and Power Fuel-Cell System

    Energy Technology Data Exchange (ETDEWEB)

    Brooks, Kriston P.; Makhmalbaf, Atefe

    2014-10-31

    Combined heat and power fuel cell systems (CHP-FCSs) provide consistent electrical power and hot water with greater efficiency and lower emissions than alternative sources. These systems can be used either as baseload, grid-connected, or as off-the-grid power sources. This report presents a technical analysis of 5 kWe CHP-FCSs installed in different locations in the U.S. At some sites as many as five 5 kWe system is used to provide up to 25kWe of power. Systems in this power range are considered “micro”-CHP-FCS. To better assess performance of micro-CHP-FCS and understand their benefits, the U.S. Department of Energy worked with ClearEdge Power to install fifteen 5-kWe PBI high temperature PEM fuel cells (CE5 models) in the commercial markets of California and Oregon. Pacific Northwest National Laboratory evaluated these systems in terms of their economics, operations, and technical performance. These units were monitored from September 2011 until June 2013. During this time, about 190,000 hours of data were collected and more than 17 billion data points were analyzed. Beginning in July 2013, ten of these systems were gradually replaced with ungraded systems (M5 models) containing phosphoric acid fuel cell technology. The new units were monitored until June 2014 until they went offline because ClearEdge was bought by Doosan at the time and the new manufacturer did not continue to support data collection and maintenance of these units. During these two phases, data was collected at once per second and data analysis techniques were applied to understand behavior of these systems. The results of this analysis indicate that systems installed in the second phase of this demonstration performed much better in terms of availability, consistency in generation, and reliability. The average net electrical power output increased from 4.1 to 4.9 kWe, net heat recovery from 4.7 to 5.4 kWth, and system availability improved from 94% to 95%. The average net system electric

  2. Fuel Cell Technical Team Roadmap

    Energy Technology Data Exchange (ETDEWEB)

    None

    2013-06-01

    The Fuel Cell Technical Team promotes the development of a fuel cell power system for an automotive powertrain that meets the U.S. DRIVE Partnership (United States Driving Research and Innovation for Vehicle efficiency and Energy sustainability) goals.

  3. Ultrastructural analysis of different human mesenchymal stem cells after in vitro expansion: a technical review

    Directory of Open Access Journals (Sweden)

    M. Miko

    2015-10-01

    Full Text Available Transmission electron microscopy reveals ultrastructural details of cells, and it is a valuable method for studying cell organelles. That is why we used this method for detailed morphological description of different adult tissuederived stem cells, focusing on the morphological signs of their functions (proteosynthetic activity, exchange with external environment, etc. and their comparison. Preparing a specimen from the cell culture suitable for transmission electron microscopy is, however, much more challenging than routine tissue processing for normal histological examination. There are several issues that need to be solved while working with cell pellets instead of solid tissue. Here we describe a simple protocol for the isolation and culture of mesenchymal stem cells from different adult tissues, with applications to stem cell biology and regenerative medicine. Since we are working with population of cells that was obtained after many days of passaging, very efficient and gentle procedures are highly necessary. We demonstrated that our semi-conservative approach regarding to histological techniques and processing of cells for transmission electron microscopy is a well reproducible procedure which results in quality pictures and images of cell populations with minimum distortions and artifacts. We also commented about riskiest steps and histochemical issues (e.g., precise pH, temperature while preparing the specimen. We bring full and detailed procedures of fixation, post-fixation, infiltration, embedding, polymerization and contrasting of cell obtained from in vitro cell and tissue cultures, with modifications according to our experience. All this steps are essential for us to know more about adult stem cells derived from different sources or about other random cell populations. The knowledge about detailed ultra-structure of adult stem cells cultured in vitro are also essential for their using in regenerative medicine and tissue engineering.

  4. Technical analysis and individual investors

    NARCIS (Netherlands)

    Hoffmann, A.O.I.; Shefrin, H.

    2014-01-01

    We find that individual investors who use technical analysis and trade options frequently make poor portfolio decisions, resulting in dramatically lower returns than other investors. The data on which this claim is based consists of transaction records and matched survey responses of a sample of Dut

  5. Humanitarian Assistance in Syria Analysis Technical Review

    Science.gov (United States)

    2014-12-01

    TRAC-M-TM-15-012 December 2014 Humanitarian Assistance in Syria Analysis Technical Review TRADOC Analysis...INTENTIONALLY LEFT BLANK TRAC-M-TM-14- December 2014 Humanitarian Assistance in Syria Analysis Technical Review Author Mr...2014 3. REPORT TYPE AND DATES COVERED Technical Memorandum, July 2014 to October 2014 4. TITLE AND SUBTITLE Humanitarian Assistance in Syria Analysis

  6. 64-row MDCT perfusion of head and neck squamous cell carcinoma: technical feasibility and quantitative analysis of perfusion parameters

    Energy Technology Data Exchange (ETDEWEB)

    Faggioni, Lorenzo; Neri, Emanuele; Cerri, Francesca; Picano, Eugenia; Bartolozzi, Carlo [University of Pisa, Diagnostic and Interventional Radiology, Pisa (Italy); Seccia, Veronica; Muscatello, Luca; Franceschini, Stefano Sellari [University of Pisa, Division of Otorhinolaryngology, Pisa (Italy)

    2011-01-15

    To evaluate the technical feasibility of 64-row computed tomography (CT) quantitative perfusion imaging of head and neck squamous cell carcinoma (SCC). Twenty-nine patients with a total of 29 pathologically proven SCC underwent a cine-mode CT perfusion acquisition covering the lesion site. The acquisition started 10 s after intravenous injection of iodinated contrast material and lasted 50 s. On a dedicated workstation, regions of interest (ROI) were traced within the SCC, on a healthy portion of tissue (H), and on the ipsilateral sternocleidomastoid muscle (M). Blood flow (BF), blood volume (BV), mean transit time (MTT) and permeability-surface product (PS) were calculated. In SCC, BF, BV and PS were higher compared with H (p < 0.0001, p = 0.002 and p = 0.004, respectively) and M (p < 0.0001). Conversely, MTT was lower in SCC than in H (p = 0.0009) and M (p = 0.0003). All datasets were free from substantial motion artefacts and ROI misregistration phenomena. No substantial discomfort or adverse events were experienced by any of the patients. 64-row CT quantitative perfusion imaging allows head and neck SCC to be distinguished from normal tissues. (orig.)

  7. Forex markets: technical analysis and algorithmic trading

    OpenAIRE

    Klepić, Igor

    2013-01-01

    Technical analysis is an efficient way for analysing financial markets such as forex where currencies are being traded. Forex market is the biggest market among all of them which is closed only during weekends. Analysis uses past behaviour of the market and with different mathematical methods tries to predict the future movement of the market. With the help of technical analysis forex market allows individuals as well as large financial corporations to efficiently manage their wealth while th...

  8. Technical Analysis of Hydrogen Production

    Energy Technology Data Exchange (ETDEWEB)

    Ali T-Raissi

    2005-01-14

    The aim of this work was to assess issues of cost, and performance associated with the production and storage of hydrogen via following three feedstocks: sub-quality natural gas (SQNG), ammonia (NH{sub 3}), and water. Three technology areas were considered: (1) Hydrogen production utilizing SQNG resources, (2) Hydrogen storage in ammonia and amine-borane complexes for fuel cell applications, and (3) Hydrogen from solar thermochemical cycles for splitting water. This report summarizes our findings with the following objectives: Technoeconomic analysis of the feasibility of the technology areas 1-3; Evaluation of the hydrogen production cost by technology areas 1; and Feasibility of ammonia and/or amine-borane complexes (technology areas 2) as a means of hydrogen storage on-board fuel cell powered vehicles. For each technology area, we reviewed the open literature with respect to the following criteria: process efficiency, cost, safety, and ease of implementation and impact of the latest materials innovations, if any. We employed various process analysis platforms including FactSage chemical equilibrium software and Aspen Technologies AspenPlus and HYSYS chemical process simulation programs for determining the performance of the prospective hydrogen production processes.

  9. Teaching audience analysis to the technical student

    Science.gov (United States)

    Debs, M. B.; Brillhart, L. V.

    1981-01-01

    Teaching audience analysis, as practiced in a technical writing course for engineering students, is discussed. Audience analysis is described as the task of defining the audience for a particular piece of writing and determining those characteristics of the audience which constrain the writer and effect reception of the message. A mature technical writing style that shows the tension produced when a text is written to be read and understood is considered in terms of audience analysis. Techniques include: (1) conveying to students the concept that a reader with certain expectations exist, (2) team teaching to preserve the context of a given technical discipline, and (3) assigning a technical report that addresses a variety of readers, thus establishing the complexity of audience oriented writing.

  10. Technical analysis of fuel cells applied to cogeneration systems; Analise tecnica de celulas combustivel aplicadas em sistemas de cogeracao

    Energy Technology Data Exchange (ETDEWEB)

    Pulgar, Rafael Girardi; Bazzo, Edson [Santa Catarina Univ., Florianopolis, SC (Brazil). Dept. de Engenharia Mecanica]. E-mails: rafael@cet.ufsc.br; ebazzo@emc.ufsc.br; Martinac, Ivo [KTH - Royal Institute of Tech., Stockholm (Sweden). Dept. of Energy Technology]. E-mail: im@egi.kth.se

    2002-07-01

    In the last years the fuel cell technology has been pointed out as a promising alternative for electricity generation. Compared to conventional energy conversion processes it presents great advantages, such as high efficiency, low gases emissions and the possibility to be applied in cogeneration systems, among others. The fuel cell converts the chemical energy of the fuel directly into electricity via an electrochemical process. The waste heat generated in the process can be recovered in cogeneration applications. In this article, preliminary results related to a Phosphoric Acid Fuel cell (PAFC) applied to hotels for power generation and hot water production are presented. The PAFC shows an electrical efficiency of about 40 per cent for power generation. In the cogeneration system proposed in this work, the global thermoelectric efficiency increased up to 73 per cent, when both electrical and thermal energy are considered. (author)

  11. Officer Overexecution: Analysis and Solutions (Technical Background)

    Science.gov (United States)

    2015-08-01

    Distribution unlimited Officer Overexecution: Analysis and Solutions (Technical Background ) Jared M. Huff and Ann D. Parcell...Deviation SME Subject Matter Expert SWO Surface Warfare Officer SWO(N) Surface Warfare Officer with the Nuclear Subspecialty TFMMS Total Force...affect retention behavior. Some subject matter experts ( SMEs ) have suggested that an excess of junior officers (JOs) on board surface ships may not

  12. Technical report on galvanic cells with fused-salt electrolytes

    Science.gov (United States)

    Cairns, E. J.; Crouthamel, C. E.; Fischer, A. K.; Foster, M. S.; Hesson, J. C.; Johnson, C. E.; Shimotake, H.; Tevebaugh, A. D.

    1969-01-01

    Technical report is presented on sodium and lithium cells using fused salt electrolytes. It includes a discussion of the thermally regenerative galvanic cell and the secondary bimetallic cell for storage of electricity.

  13. 77 FR 50488 - Hydrogen and Fuel Cell Technical Advisory Committee

    Science.gov (United States)

    2012-08-21

    ... Hydrogen and Fuel Cell Technical Advisory Committee AGENCY: Department of Energy, Office of Energy... open meeting (Webinar) of the Hydrogen and Fuel Cell Technical Advisory Committee (HTAC). The Federal..., DC 20585. SUPPLEMENTARY INFORMATION: Purpose of the Committee: The Hydrogen and Fuel Cell...

  14. Stock market prediction using technical analysis

    Directory of Open Access Journals (Sweden)

    Bradić-Martinović Aleksandra

    2006-01-01

    Full Text Available Technical analysis (TA is a form of analyzing market encompassing supply and demand of securities according to the study of their prices and trading volume. Using the appropriate methods, TA aims to identify price movements in the stock market, futures or currencies. In short, TA analysis is the process by which "future price movements are formulated according to the price history". TA originates from the work of Charles Dow and his conclusions about the global behavior of the market, as well as from Elliot Wave Theory. Dow did not regard its theory as a tool for stock market movement prediction, nor as a guide for investors, but as a kind of barometer of general market movements. The term TA methods encompasses all the methods used in tracking prices aiming to clearly predict future events. Many different methods, mainly statistical, are used in technical analysis, the most popular ones being: establishing and following trends using moving average, recognizing price momentum, calculating indicators and oscillators, as well as cycle analysis (structure indicators. It is also necessary to point out that TA is not a science in the true meaning of the term, and that methods it uses frequently deviate from the conventional manner of their use. The main advantage of these methods is their relative ease of use, aiming to give as clear picture as possible of price movements, while at the same time avoiding the use of complicated and complex mathematical methods. The reason for this is simple and is reflected in the dynamics of financial markets, where changes occur during short periods of time and where prompt decision-making is of vital importance.

  15. Formal modelling and analysis of socio-technical systems

    DEFF Research Database (Denmark)

    Probst, Christian W.; Kammüller, Florian; Hansen, Rene Rydhof

    2016-01-01

    -technical systems are still mostly identified through brainstorming of experts. In this work we discuss several approaches to formalising socio-technical systems and their analysis. Starting from a flow logic-based analysis of the insider threat, we discuss how to include the socio aspects explicitly, and show...

  16. Multidirectional analysis of technical efficiency for pig production systems

    DEFF Research Database (Denmark)

    Labajavo, Katarina; Hansson, Helena; Asmild, Mette;

    2016-01-01

    Declining profitability and ongoing structural changes in the pig sector require thorough efficiency analysis of individual production factors. In this study we calculated technical efficiency indices for each input and output using multidirectional efficiency analysis and examined the relationsh...

  17. [Hepatic cell transplantation. Technical and methodological aspects].

    Science.gov (United States)

    Pareja, Eugenia; Martínez, Amparo; Cortés, Miriam; Bonora, Ana; Moya, Angel; Sanjuán, Fernando; Gómez-Lechón, M José; Mir, José

    2010-03-01

    Hepatic cell transplantation consists of grafting already differentiated cells such as hepatocytes. Human hepatocytes are viable and functionally active. Liver cell transplantation is carried out by means of a 3-step method: isolation of hepatocytes from donor liver rejected for orthotopic transplantation, preparing a cell suspension for infusion and, finally, hepatocytes are implanted into the recipient. There are established protocols for the isolation of human hepatocytes from unused segments of donor livers, based on collagenase digestion of cannulated liver tissue at 37 degrees C. The hepatocytes can be used fresh or cryopreserved. Cryopreservation of isolated human hepatocytes would then be available for planned use. In cell transplant, the important aspects are: infusion route, number of cells, number of infusions and viability of the cells. The cells are infused into the patient through a catheter inserted via portal vein or splenic artery. Liver cell transplantation allows liver tissue to be used that would, otherwise, be discarded, enabling multiple patients to be treated with hepatocytes from a single tissue donor.

  18. Technical Challenges in the Derivation of Human Pluripotent Cells

    Directory of Open Access Journals (Sweden)

    Parinya Noisa

    2011-01-01

    Full Text Available It has long been discovered that human pluripotent cells could be isolated from the blastocyst state of embryos and called human embryonic stem cells (ESCs. These cells can be adapted and propagated indefinitely in culture in an undifferentiated manner as well as differentiated into cell representing the three major germ layers: endoderm, mesoderm, and ectoderm. However, the derivation of human pluripotent cells from donated embryos is limited and restricted by ethical concerns. Therefore, various approaches have been explored and proved their success. Human pluripotent cells can also be derived experimentally by the nuclear reprogramming of somatic cells. These techniques include somatic cell nuclear transfer (SCNT, cell fusion and overexpression of pluripotent genes. In this paper, we discuss the technical challenges of these approaches for nuclear reprogramming, involving their advantages and limitations. We will also highlight the possible applications of these techniques in the study of stem cell biology.

  19. Analysis of "Babar Loses His Crown." Technical Report No. 169.

    Science.gov (United States)

    Green, G. M.; And Others

    This report presents the text analysis of "Babar Loses His Crown," a story for beginning readers. (The techniques used in arriving at the analysis are presented in a Reading Center Technical Report, Number 168, "Problems and Techniques of Text Analysis.") Tables are given for a statistical lexical analysis and for a syntactic…

  20. Chilean Nominal Exchange Rate: Forecasting Based Upon Technical Analysis

    OpenAIRE

    Ana María Abarca; Felipe Alarcón; Pablo Pincheira; Jorge Selaive

    2007-01-01

    : This work presents a review of the main indicators used in the technical analysis of the peso-dollar parity. We explain the interpretations carried out by technical analysts of these indicators and perform forecasting analysis of the Relative Strength Index (RSI) to predict exchange rate returns at daily frequency. The predictive exercises are done using both in-sample and out-of-sample techniques, and report a robust forecasting ability for horizons within 7 weeks.

  1. Solar Cell Nanotechnology Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Das, Biswajit [Univ. of Nevada, Las Vegas, NV (United States)

    2014-05-07

    The objective of this project is to develop a low cost nonlithographic nanofabrication technology for the fabrication of thin film porous templates as well as uniform arrays of semiconductor nanostructures for the implementation of high efficiency solar cells. Solar cells based on semiconductor nanostructures are expected to have very high energy conversion efficiencies due to the increased absorption coefficients of semiconductor nanostructures. In addition, the thin film porous template can be used for optimum surface texturing of solar cells leading to additional enhancement in energy conversion efficiency. An important requirement for these applications is the ability to synthesize nanostructure arrays of different dimensions with good size control. This project employed nanoporous alumina templates created by the anodization of aluminum thin films deposited on glass substrates for the fabrication of the nanostructures and optimized the process parameters to obtain uniform pore diameters. An additional requirement is uniformity or regularity of the nanostructure arrays. While constant current anodization was observed to provide controlled pore diameters, constant voltage anodization was needed for regularity of the nanostructure arrays. Thus a two-step anodization process was investigated and developed in this project for improving the pore size distribution and pore periodicity of the nanoporous alumina templates. CdTe was selected to be the active material for the nanowires, and the process for the successful synthesis of CdTe nanowires was developed in this project. Two different synthesis approaches were investigated in this project, electrochemical and electrophoretic deposition. While electrochemical synthesis was successfully employed for the synthesis of nanowires inside the pores of the alumina templates, the technique was determined to be non-optimum due to the need of elevated temperature that is detrimental to the structural integrity of the

  2. Recent key technical barriers in solid oxide fuel cell technology

    Directory of Open Access Journals (Sweden)

    Milewski Jarosław

    2014-03-01

    Full Text Available High-temperature solid oxide fuel cells (SOFCs are considered as suitable components of future large-scale clean and efficient power generation systems. However, at its current stage of development some technical barriers exists which limit SOFC’s potential for rapid large-scale deployment. The present article aims at providing solutions to key technical barriers in SOFC technology. The focus is on the solutions addressing thermal resistance, fuel reforming, energy conversion efficiency, materials, design, and fuel utilisation issues.

  3. Preliminary Technical Risk Analysis for the Geothermal Technologies Program

    Energy Technology Data Exchange (ETDEWEB)

    None

    2009-01-18

    This report explains the goals, methods, and results of a probabilistic analysis of technical risk for a portfolio of R&D projects in the DOE Geothermal Technologies Program (The Program). The analysis is a task by Princeton Energy Resources International, LLC, in support of the National Renewable Energy Laboratory on behalf of the Program. The main challenge in the analysis lies in translating R&D results to a quantitative reflection of technical risk for a key Program metric: levelized cost of energy (LCOE).

  4. 78 FR 18578 - Hydrogen and Fuel Cell Technical Advisory Committee (HTAC)

    Science.gov (United States)

    2013-03-27

    ... of Energy Efficiency and Renewable Energy Hydrogen and Fuel Cell Technical Advisory Committee (HTAC... Meeting. SUMMARY: This notice announces an open meeting of the Hydrogen and Fuel Cell Technical Advisory.... SUPPLEMENTARY INFORMATION: Purpose of the Committee: The Hydrogen and Fuel Cell Technical Advisory...

  5. Content Analysis as a Best Practice in Technical Communication Research

    Science.gov (United States)

    Thayer, Alexander; Evans, Mary; McBride, Alicia; Queen, Matt; Spyridakis, Jan

    2007-01-01

    Content analysis is a powerful empirical method for analyzing text, a method that technical communicators can use on the job and in their research. Content analysis can expose hidden connections among concepts, reveal relationships among ideas that initially seem unconnected, and inform the decision-making processes associated with many technical…

  6. Hydrogen Technical Analysis -- Dissemination of Information

    Energy Technology Data Exchange (ETDEWEB)

    George Kervitsky, Jr.

    2006-03-20

    SENTECH is a small energy and environmental consulting firm providing technical, analytical, and communications solutions to technology management issues. The activities proposed by SENTECH focused on gathering and developing communications materials and information, and various dissemination activities to present the benefits of hydrogen energy to a broad audience while at the same time establishing permanent communications channels to enable continued two-way dialog with these audiences in future years. Effective communications and information dissemination is critical to the acceptance of new technology. Hydrogen technologies face the additional challenge of safety preconceptions formed primarily as a result of the crash of the Hindenburg. Effective communications play a key role in all aspects of human interaction, and will help to overcome the perceptual barriers, whether of safety, economics, or benefits. As originally proposed SENTECH identified three distinct information dissemination activities to address three distinct but important audiences; these formed the basis for the task structure used in phases 1 and 2. The tasks were: (1) Print information--Brochures that target the certain segment of the population and will be distributed via relevant technical conferences and traditional distribution channels. (2) Face-to-face meetings--With industries identified to have a stake in hydrogen energy. The three industry audiences are architect/engineering firms, renewable energy firms, and energy companies that have not made a commitment to hydrogen (3) Educational Forums--The final audience is students--the future engineers, technicians, and energy consumers. SENTECH will expand on its previous educational work in this area. The communications activities proposed by SENTECH and completed as a result of this cooperative agreement was designed to compliment the research and development work funded by the DOE by presenting the technical achievements and validations

  7. Error Analysis in Mathematics. Technical Report #1012

    Science.gov (United States)

    Lai, Cheng-Fei

    2012-01-01

    Error analysis is a method commonly used to identify the cause of student errors when they make consistent mistakes. It is a process of reviewing a student's work and then looking for patterns of misunderstanding. Errors in mathematics can be factual, procedural, or conceptual, and may occur for a number of reasons. Reasons why students make…

  8. Regulatory analysis technical evaluation handbook. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1997-01-01

    The purpose of this Handbook is to provide guidance to the regulatory analyst to promote preparation of quality regulatory analysis documents and to implement the policies of the Regulatory Analysis Guidelines of the US Nuclear Regulatory Commission (NUREG/BR-0058 Rev. 2). This Handbook expands upon policy concepts included in the NRC Guidelines and translates the six steps in preparing regulatory analyses into implementable methodologies for the analyst. It provides standardized methods of preparation and presentation of regulatory analyses, with the inclusion of input that will satisfy all backfit requirements and requirements of NRC`s Committee to Review Generic Requirements. Information on the objectives of the safety goal evaluation process and potential data sources for preparing a safety goal evaluation is also included. Consistent application of the methods provided here will result in more directly comparable analyses, thus aiding decision-makers in evaluating and comparing various regulatory actions. The handbook is being issued in loose-leaf format to facilitate revisions. NRC intends to periodically revise the handbook as new and improved guidance, data, and methods become available.

  9. Preliminary Technical Risk Analysis for the Geothermal Technologies Program

    Energy Technology Data Exchange (ETDEWEB)

    McVeigh, J.; Cohen, J.; Vorum, M.; Porro, G.; Nix, G.

    2007-03-01

    This report explains the goals, methods, and results of a probabilistic analysis of technical risk for a portfolio of R&D projects in the DOE Geothermal Technologies Program ('the Program'). The analysis is a task by Princeton Energy Resources International, LLC (PERI), in support of the National Renewable Energy Laboratory (NREL) on behalf of the Program. The main challenge in the analysis lies in translating R&D results to a quantitative reflection of technical risk for a key Program metric: levelized cost of energy (LCOE). This requires both computational development (i.e., creating a spreadsheet-based analysis tool) and a synthesis of judgments by a panel of researchers and experts of the expected results of the Program's R&D.

  10. Technical Efficiency of Thai Manufacturing SMEs: A Stochastic Frontier Analysis

    Directory of Open Access Journals (Sweden)

    Teerawat Charoenrat

    2013-03-01

    Full Text Available AbstractA major motivation of this study is to examine the factors that are the most important in contributing to the relatively poor efficiency performance of Thai manufacturing small and medium sized enterprises (SMEs. The results obtained will be significant in devising effective policies aimed at tackling this poor performance.This paper uses data on manufacturing SMEs in the North-eastern region of Thailand in 2007 as a case study, by applying a stochastic frontier analysis (SFA and a technical inefficiency effects model. The empirical results obtained indicate that the mean technical efficiency of all categories of manufacturing SMEs in theNorth-eastern region is 43%, implying that manufacturing SMEs have high levels of technical inefficiency in their production processes.Manufacturing SMEs in the North-eastern region are particularly labour-intensive. The empirical results of the technical inefficiency effects model suggest that skilled labour, the municipal area and ownership characteristics are important firm-specific factors affecting technical efficiency. The paper argues that the government should play a more substantial role in developing manufacturing SMEs in the North-eastern provinces through: providing training programs for employees and employers; encouraging a greater usage of capital and technology in the production process of SMEs; enhancing the efficiency of state-ownedenterprises; encouraging a wide range of ownership forms; and improving information and communications infrastructure.

  11. DNDO Analysis Cell Concept

    Energy Technology Data Exchange (ETDEWEB)

    Pagh, Richard T.; Dimmerling, Paul J.; Guillen, Zoe C.; Hoyt, Joel R.; Jarman, Kenneth D.; Kernan, Warnick J.; Reichmuth, Barbara A.; Rohlfing, Kerrie S.; Schweppe, John E.; Sego, Landon H.; Shergur, Jason M.; Siciliano, Edward R.; Woodring, Mitchell L.

    2014-03-12

    The Domestic Nuclear Detection Office (DNDO) has a mission of implementing rad/nuc interdiction capabilities for a managed and coordinated response to threats, integration of federal nuclear forensics programs, and coordinating the development of the global nuclear detection and reporting architecture. In the process of executing this mission, DNDO has generated substantial information, data, technical results, operational workflows and analytical tools. The effective utilization of these resources is an overarching goal of the organization. After nearly a decade of performing work, DNDO faces a challenge in capitalizing on the large amount of data, reports, processes, tools, and people. As new work is being planned, managers and researchers need to have an understating of what information has been collected, what tools are available, the collaborations which can be utilized to propel the work forward, processes to plan and execute, and how to present conclusions and results that can assist the government in making decisions. This type of challenge can be met through the use of a series of organized and connected elements which form a broader structure (cell) that promotes cross utilization of elements such that they can be tailored (analyzed) to fit the context of the problem to be solved. The development of an analysis cell for DNDO will address the challenges of utilizing existing elements, identifying gaps, annually reporting the performance of rad/nuc interdiction instrumentation, and planning the execution of future work.

  12. 78 FR 6086 - Hydrogen and Fuel Cell Technical Advisory Committee (HTAC)

    Science.gov (United States)

    2013-01-29

    ... Hydrogen and Fuel Cell Technical Advisory Committee (HTAC) AGENCY: Office of Energy Efficiency and... announces an open meeting (Webinar) of the Hydrogen and Fuel Cell Technical Advisory Committee (HTAC). The..., DC 20585. SUPPLEMENTARY INFORMATION: Purpose of the Committee: The Hydrogen and Fuel Cell...

  13. Applications and technical challenges of fluorescence in situhybridization in stem cell research

    Energy Technology Data Exchange (ETDEWEB)

    Weier, Heinz-Ulli G.; Chu, Lisa W.; Murnane, John P.; Weier,Jingly F.

    2003-07-02

    Stem cell research, maintenance and manipulations have advanced significantly in recent years, and we now witness successful clinical applications of stem therapies. However, challenges in regard to karyotypic stability and the ploidy status of stem cell lines have been addressed only marginally. Our approach to develop technology to address these highly relevant issues is based on fluorescence in situ hybridization (FISH) using non-isotopically labeled DNA probes. As a single cell analysis technique, FISH is expected to be applicable to a variety of cells and tissues including interphase and metaphase cell preparations as well as tissue sections and biopsy material. Over the last decade, our laboratories generated a large number of probes and probe sets for the molecular cytogenetic analyses of stem cells derived from different species. These probes and the introduction of Spectral Imaging bring us close to be able to perform a comprehensive karyotype analysis of single interphase cell nuclei. It should furthermore be possible to couple cytogenetic investigations of the cellular genotype with analysis of gene expression. This report summarizes our technical achievements relevant to stem cell research, and outlines plans for future research and developments.

  14. Multivariate analysis of identity of imported technical PCN formulation.

    Science.gov (United States)

    Falandysz, J; Chudzyński, K; Takekuma, M; Yamamoto, T; Noma, Y; Hanari, N; Yamashita, N

    2008-10-01

    Chloronaphthalenes form a class of compounds consisting of 8 CN homologue groups and altogether of 75 congeners, which used have been most extensively in 1930--1950. An investigation have been performed on the possible origin of unidentified by name technical chloronaphthalene formulation unlawfully imported recently from the United Kingdom to Japan. Principal component analysis (PCA) and Cluster Analysis of chloronaphthalene congener isomer-specific and homologue classes' compositional HRGC/HRMS data of imported CN formulation and of certain brands of technical CN formulation called Halowax (Halowax 1000, 1001 and 1031) enabled to identify that unnamed product is not Halowax 1001. A less accurate multivariate examination based on CN homologue classes patter did indicate on large similarity between unlawfully imported technical CN formulation and Halowax 1001 (manufactured by the Koppers Ind. Co., USA), while a more accurate based on CN congeners pattern differentiated them as to of various origin mixtures. Based on chlorine content of imported CN formulation (50-52%) and its no similarity to Halowax 1001 it seems reasonable to conclude that unnamed CN mixture examined could be a sample of stockpiled Seekay wax R93.

  15. Pareto analysis of critical factors affecting technical institution evaluation

    Directory of Open Access Journals (Sweden)

    Victor Gambhir

    2012-08-01

    Full Text Available With the change of education policy in 1991, more and more technical institutions are being set up in India. Some of these institutions provide quality education, but others are merely concentrating on quantity. These stakeholders are in a state of confusion about decision to select the best institute for their higher educational studies. Although various agencies including print media provide ranking of these institutions every year, but their results are controversial and biased. In this paper, the authors have made an endeavor to find the critical factors for technical institution evaluation from literature survey. A Pareto analysis has also been performed to find the intensity of these critical factors in evaluation. This will not only help the stake holders in taking right decisions but will also help the management of institutions in benchmarking for identifying the most important critical areas to improve the existing system. This will in turn help Indian economy.

  16. Technical analysis of prospective photovoltaic systems in Utah.

    Energy Technology Data Exchange (ETDEWEB)

    Quiroz, Jimmy Edward; Cameron, Christopher P.

    2012-02-01

    This report explores the technical feasibility of prospective utility-scale photovoltaic system (PV) deployments in Utah. Sandia National Laboratories worked with Rocky Mountain Power (RMP), a division of PacifiCorp operating in Utah, to evaluate prospective 2-megawatt (MW) PV plants in different locations with respect to energy production and possible impact on the RMP system and customers. The study focused on 2-MW{sub AC} nameplate PV systems of different PV technologies and different tracking configurations. Technical feasibility was evaluated at three different potential locations in the RMP distribution system. An advanced distribution simulation tool was used to conduct detailed time-series analysis on each feeder and provide results on the impacts on voltage, demand, voltage regulation equipment operations, and flicker. Annual energy performance was estimated.

  17. 77 FR 65542 - Hydrogen and Fuel Cell Technical Advisory Committee (HTAC)

    Science.gov (United States)

    2012-10-29

    ... of Energy Efficiency and Renewable Energy Hydrogen and Fuel Cell Technical Advisory Committee (HTAC... Meeting. SUMMARY: The Hydrogen and Fuel Cell Technical Advisory Committee (HTAC) was established under... web at: http://hydrogen.energy.gov ). Public Comment DOE Program Updates Congressional Fuel...

  18. 77 FR 2714 - Hydrogen and Fuel Cell Technical Advisory Committee (HTAC)

    Science.gov (United States)

    2012-01-19

    ... Hydrogen and Fuel Cell Technical Advisory Committee (HTAC) AGENCY: Department of Energy, Office of Energy... open meeting of the Hydrogen and Fuel Cell Technical Advisory Committee (HTAC). The HTAC was... EPACT. Tentative Agenda: (Subject to change; updates will be posted on...

  19. 77 FR 18243 - Hydrogen and Fuel Cell Technical Advisory Committee (HTAC); Notice of Open Meeting

    Science.gov (United States)

    2012-03-27

    ... Hydrogen and Fuel Cell Technical Advisory Committee (HTAC); Notice of Open Meeting AGENCY: Office of Energy... announces a meeting of the Hydrogen and Fuel Cell Technical Advisory Committee (HTAC). The Federal Advisory...., Washington, DC 20585. SUPPLEMENTARY INFORMATION: Purpose of the Committee: The Hydrogen and Fuel...

  20. 78 FR 60866 - Hydrogen and Fuel Cell Technical Advisory Committee (HTAC)

    Science.gov (United States)

    2013-10-02

    ... Hydrogen and Fuel Cell Technical Advisory Committee (HTAC) AGENCY: Office of Energy Efficiency and... open meeting of the Hydrogen and Fuel Cell Technical Advisory Committee (HTAC). The Federal Advisory... Avenue, Washington, DC 20585. SUPPLEMENTARY INFORMATION: Purpose of the Committee: The Hydrogen and...

  1. 75 FR 26743 - Hydrogen and Fuel Cell Technical Advisory Committee (HTAC)

    Science.gov (United States)

    2010-05-12

    ... No: 2010-11288] DEPARTMENT OF ENERGY Hydrogen and Fuel Cell Technical Advisory Committee (HTAC... Meeting. SUMMARY: The Hydrogen and Fuel Cell Technical Advisory Committee (HTAC) was established under... title VIII of EPACT. Tentative Agenda: (Subject to change; updates will be posted on...

  2. 75 FR 2860 - Hydrogen and Fuel Cell Technical Advisory Committee (HTAC)

    Science.gov (United States)

    2010-01-19

    ... of Energy Efficiency and Renewable Energy Hydrogen and Fuel Cell Technical Advisory Committee (HTAC... Meeting. SUMMARY: The Hydrogen and Fuel Cell Technical Advisory Committee (HTAC) was established under... posted on http://hydrogen.energy.gov and copies of the final agenda will available the date of...

  3. 76 FR 28759 - Hydrogen and Fuel Cell Technical Advisory Committee (HTAC)

    Science.gov (United States)

    2011-05-18

    ... of Energy Efficiency and Renewable Energy Hydrogen and Fuel Cell Technical Advisory Committee (HTAC... meeting. SUMMARY: The Hydrogen and Fuel Cell Technical Advisory Committee (HTAC) was established under... address: HTAC@nrel.gov or check the Web site at: hydrogen.energy.gov . SUPPLEMENTARY INFORMATION:...

  4. 76 FR 4645 - Hydrogen and Fuel Cell Technical Advisory Committee (HTAC)

    Science.gov (United States)

    2011-01-26

    ... Hydrogen and Fuel Cell Technical Advisory Committee (HTAC) AGENCY: Department of Energy, Office of Energy... the Hydrogen and Fuel Cell Technical Advisory Committee (HTAC). HTAC was established under section 807... posted on http://hydrogen.energy.gov and copies of the final agenda will available the date of...

  5. Stochastic Volatility Model and Technical Analysis of Stock Price

    Institute of Scientific and Technical Information of China (English)

    Wei LIU; Wei An ZHENG

    2011-01-01

    In the stock market, some popular technical analysis indicators (e.g. Bollinger Bands, RSI,ROC, ...) are widely used by traders. They use the daily (hourly, weekly, ...) stock prices as samples of certain statistics and use the observed relative frequency to show the validity of those well-knownindicators. However, those samples are not independent, so the classical sample survey theory does not apply. In earlier research, we discussed the law of large numbers related to those observations when one assumes Black-Scholes' stock price model. In this paper, we extend the above results to the more popular stochastic volatility model.

  6. Feasibility of commercial space manufacturing, production of pharmaceuticals. Volume 2: Technical analysis

    Science.gov (United States)

    1978-01-01

    A technical analysis on the feasibility of commercial manufacturing of pharmaceuticals in space is presented. The method of obtaining pharmaceutical company involvement, laboratory results of the separation of serum proteins by the continuous flow electrophoresis process, the selection and study of candidate products, and their production requirements is described. The candidate products are antihemophilic factor, beta cells, erythropoietin, epidermal growth factor, alpha-1-antitrypsin and interferon. Production mass balances for antihemophelic factor, beta cells, and erythropoietin were compared for space versus ground operation. A conceptual description of a multiproduct processing system for space operation is discussed. Production requirements for epidermal growth factor of alpha-1-antitrypsin and interferon are presented.

  7. Technical analysis of photovoltaic/wind systems with hydrogen storage

    Directory of Open Access Journals (Sweden)

    Bakić Vukman V.

    2012-01-01

    Full Text Available The technical analysis of a hybrid wind-photovoltaic energy system with hydrogen gas storage was studied. The market for the distributed power generation based on renewable energy is increasing, particularly for the standalone mini-grid applications. The main design components of PV/Wind hybrid system are the PV panels, the wind turbine and an alkaline electrolyzer with tank. The technical analysis is based on the transient system simulation program TRNSYS 16. The study is realized using the meteorological data for a Typical Metrological Year (TMY for region of Novi Sad, Belgrade cities and Kopaonik national park in Serbia. The purpose of the study is to design a realistic energy system that maximizes the use of renewable energy and minimizes the use of fossil fuels. The reduction in the CO2 emissions is also analyzed in the paper. [Acknowledgment. This paper is the result of the investigations carried out within the scientific project TR33036 supported by the Ministry of Science of the Republic of Serbia.

  8. Technical and financial analysis of combined cycle gas turbine

    Directory of Open Access Journals (Sweden)

    Khan Arshad Muhammad

    2013-01-01

    Full Text Available This paper presents technical and financial models which were developed in this study to predict the overall performance of combined cycle gas turbine plant in line with the needs of independent power producers in the liberalized market of power sector. Three similar sizes of combined cycle gas turbine power projects up to 200 Megawatt of independent power producers in Pakistan were selected in-order to develop and drive the basic assumptions for the inputs of the models in view of prevailing Government of Pakistan’s two components of electricity purchasing tariff that is energy purchase price and capacity purchase price at higher voltage grid station terminal from independent power producers. The levelized electricity purchasing tariff over life of plant on gaseous fuel at 60 percent plant load factor was 6.47 cent per kilowatt hour with energy purchase price and capacity purchase prices of 3.54 and 2.93 cents per kilowatt hour respectively. The outcome of technical models of gas turbine, steam turbine and combined cycle gas turbine power were found in close agreement with the projects under consideration and provides opportunity of evaluation of technical and financial aspects of combined cycle power plant in a more simplified manner with relatively accurate results. At 105 Celsius exit temperature of heat recovery steam generator flue gases the net efficiency of combined cycle gas turbine was 48.8 percent whereas at 125 Celsius exit temperature of heat recovery steam generator flue gases it was 48.0 percent. Sensitivity analysis of selected influential components of electricity tariff was also carried out.

  9. Trading Volume and Stock Indices: A Test of Technical Analysis

    Directory of Open Access Journals (Sweden)

    Paul Abbondante

    2010-01-01

    Full Text Available Problem statement: Technical analysis and its emphasis on trading volume has been used to analyze movements in individual stock prices and make investment recommendations to either buy or sell that stock. Little attention has been paid to investigating the relationship between trading volume and various stock indices. Approach: Since stock indices track overall stock market movements, trends in trading volume could be used to forecast future stock market trends. Instead of focusing only on individual stocks, this study will examine movements in major stock markets as a whole. Regression analysis was used to investigate the relationship between trading volume and five popular stock indices using daily data from January, 2000 to June, 2010. A lag of 5 days was used because this represents the prior week of trading volume. The total sample size ranges from 1,534-2,638 to observations. Smaller samples were used to test the investment horizon that explains movements of the indices more completely. Results: The F statistics were significant for samples using 6 and 16 months of data. The F statistic was not significant using a sample of 1 month of data. This is surprising given the short term focus of technical analysis. The results indicate that above-average returns can be achieved using futures, options and exchange traded funds which track these indices. Conclusion: Future research efforts will include out-of-sample forecasting to determine if above-average returns can be achieved. Additional research can be conducted to determine the optimal number of lags for each index.

  10. Molten Carbonate and Phosphoric Acid Stationary Fuel Cells: Overview and Gap Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Remick, R.; Wheeler, D.

    2010-09-01

    This report describes the technical and cost gap analysis performed to identify pathways for reducing the costs of molten carbonate fuel cell (MCFC) and phosphoric acid fuel cell (PAFC) stationary fuel cell power plants.

  11. FUNDAMENTAL AND TECHNICAL ANALYSIS ON CROATIAN STOCK MARKET

    Directory of Open Access Journals (Sweden)

    Veronika Čaljkušić

    2011-02-01

    Full Text Available The main subject of this paper is to find the right approach to the evaluation of stock and predicting the moment in which investors should take action by using different approaches and methods during a certain period of time. This analysis has been made by using stock prices quoted on the Zagreb Stock Exchange (ZSE. This paper identifies how fast and to what extent turbulences in the global economy may have an impact on Croatian stock market. In the paper are estimated situations in which it is better to rely more on indicators of fundamental analysis than on technical analysis and vice-versa. It is also estimated which indicators should be used as the support to these two analyses. In the paper were explored the movements of the stock prices and CROBEX index and whether they can be used as the signal of a “cyclone” in the economy or presented as the result of a slow down in the economy.

  12. Analysis of Software Design Artifacts for Socio-Technical Aspects

    OpenAIRE

    Damaševičius, Robertas; Kaunas University of Technology

    2007-01-01

    Software systems are not purely technical objects. They are designed, constructed and used by people. Therefore, software design process is not purely a technical task, but a socio-technical process embedded within organizational and social structures. These social structures influence and govern their work behavior and final work products such as program source code and documentation. This paper discusses the organizational, social and psychological aspects of software design; and formulates...

  13. Biosensors for Cell Analysis.

    Science.gov (United States)

    Zhou, Qing; Son, Kyungjin; Liu, Ying; Revzin, Alexander

    2015-01-01

    Biosensors first appeared several decades ago to address the need for monitoring physiological parameters such as oxygen or glucose in biological fluids such as blood. More recently, a new wave of biosensors has emerged in order to provide more nuanced and granular information about the composition and function of living cells. Such biosensors exist at the confluence of technology and medicine and often strive to connect cell phenotype or function to physiological or pathophysiological processes. Our review aims to describe some of the key technological aspects of biosensors being developed for cell analysis. The technological aspects covered in our review include biorecognition elements used for biosensor construction, methods for integrating cells with biosensors, approaches to single-cell analysis, and the use of nanostructured biosensors for cell analysis. Our hope is that the spectrum of possibilities for cell analysis described in this review may pique the interest of biomedical scientists and engineers and may spur new collaborations in the area of using biosensors for cell analysis.

  14. On The Human, Organizational, and Technical Aspects of Software Development and Analysis

    Science.gov (United States)

    Damaševičius, Robertas

    Information systems are designed, constructed, and used by people. Therefore, a software design process is not purely a technical task, but a complex psycho-socio-technical process embedded within organizational, cultural, and social structures. These structures influence the behavior and products of the programmer's work such as source code and documentation. This chapter (1) discusses the non-technical (organizational, social, cultural, and psychological) aspects of software development reflected in program source code; (2) presents a taxonomy of the social disciplines of computer science; and (3) discusses the socio-technical software analysis methods for discovering the human, organizational, and technical aspects embedded within software development artifacts.

  15. Teaching technical writing in multilingual contexts: a meta-analysis

    NARCIS (Netherlands)

    Winberg, Christine; Geest, van der Thea; Lehman, Barbara; Nduna, Joyce

    2010-01-01

    Teachers of technical and professional writing in Science, Engineering and Technology (SET) Programmes need to understand the particular needs and social contexts of students for whom English is not a first language. The focus of this paper is on technical writing, and the paper presents the finding

  16. Geospatial Analysis of Renewable Energy Technical Potential on Tribal Lands

    Energy Technology Data Exchange (ETDEWEB)

    Doris, E.; Lopez, A.; Beckley, D.

    2013-02-01

    This technical report uses an established geospatial methodology to estimate the technical potential for renewable energy on tribal lands for the purpose of allowing Tribes to prioritize the development of renewable energy resources either for community scale on-tribal land use or for revenue generating electricity sales.

  17. Technical-tactical analysis of youth olympic taekwondo combat.

    Science.gov (United States)

    Tornello, Francesco; Capranica, Laura; Minganti, Carlo; Chiodo, Salvatore; Condello, Giancarlo; Tessitore, Antonio

    2014-04-01

    The purpose of this study was to define the technical and tactical profiles of official youth taekwondo competitions played under the most recent rules of the International Taekwondo Federation. Tactical actions (i.e., attack, defense, and block), technical executions (from 1- to 4-point scores), kicking legs (i.e., front/rear and right/left), and overall technical effectiveness were investigated in relation to match outcome of semifinal and final competitions (n = 50) of youth (aged 13-14 years) black belt athletes during the Italian Taekwondo Cadet Championship. Differences (p technical exchanges showed differences (p technical executions. Winners resulted more efficient (p technical and tactical variables. In general, these findings showed that Cadets tend to adopt an offensive strategy. In considering that the adoption of the new electronic system requires athletes to execute correct technical actions to have a score assigned, coaches should emphasize the effectiveness of scoring techniques and help athletes to effectively improve their defense and counterattack capabilities.

  18. Final Technical Report Advanced Solar Resource Modeling and Analysis.

    Energy Technology Data Exchange (ETDEWEB)

    Hansen, Clifford [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-12-01

    The SunShot Initiative coordinates research, development, demonstration, and deployment activities aimed at dramatically reducing the total installed cost of solar power. The SunShot Initiative focuses on removing critical technical and non-technical barriers to installing and integrating solar energy into the electricity grid. Uncertainty in projected power and energy production from solar power systems contributes to these barriers by increasing financial risks to photovoltaic (PV) deployment and by exacerbating the technical challenges to integration of solar power on the electricity grid.

  19. Towards MOOC for Technical Courses: A Blended Learning Empirical Analysis

    Directory of Open Access Journals (Sweden)

    Siti Feirusz Ahmad Fesol

    2016-12-01

    Full Text Available Massive Open Online Learning (MOOC is one of the rapidly growing and the most trending online learning platform throughout the world. As reported by Class Central up until December 2015, there are more than a total of 4200 courses, which enrolled more than 35 million students and adopted by more than 500 universities all over the world. Thus, the objective of this study is to identify the students’ readiness towards MOOC technical courses based on blended learning approach. This study adapted quantitative based approach to analyse the data gathered.  Descriptive analysis and factor analysis are used to empirically analyse a total of 39 items on student attitude towards blended learning. This study successfully in developing six dimensions of student attitude towards the implementation of MOOC learning. The attributes namely are attitude towards learning flexibility, online learning, study management, technology, online interaction, and classroom learning. The findings summarized that, when students had a positive attitude towards learning flexibility, online learning, study management, technology, and online interaction, the students were more likely to adapt to blended learning and highly ready towards MOOC learning. On the other hand, when students had a positive attitude towards classroom learning, they were less likely ready towards MOOC learning, as they would prefer to meet their lecturers and friends in a physical lecture class compared to on the web-based. Understanding of student’s readiness towards MOOC learning based on blended learning approach is one of the critical success factors for implementing successful MOOC by higher learning institutions.

  20. Accuracy Combination Test of Classical and Modern Technical Analysis: A Case Study in Stock of PT Wijaya Karya Tbk

    Directory of Open Access Journals (Sweden)

    Agustini Hamid

    2016-05-01

    Full Text Available The research aimed to measure the accuracy and combination of Classic and Modern Technical Analysis. PT Wijaya Karya Tbk (WIKA’s stock in two periods is the sample of research. Technical analysis was used to predict stock prices by observing changes in historical share price. Practically, technical analysis is divided into Classic Technical and Modern. Research was conducted by library study and using a computer software. Microsft Excel was used for the simulation and Chart Nexus for analyzing Modern Technical Analysis. The research period started in January 1, 2013 until December 31, 2013 and January 1, 2014 until December 31, 2014. The Classic Technical Analysis used Support, Resistance, Trendline, and Flag Patern. Meanwhile for Modern Technical Analysis used Moving Average, Stochastic, Moving Average Convergence Divergence (MACD indicator. The Classical Technical Analysis gave less result than Modern Technical Analysis. The classical give 14 investment decisions in two periods. The average return of Classical Technical is 15,50%. Meanwhile the Modern Technical Analysis gave 18 investment decisions in two periods. The average return of Modern Technical is 18,14%. Combining Classic Technical Analysis and Modern Technical Analysis gave 20 investment decisions with the average rate of return 20,41%.

  1. Descriptive analysis of context evaluation instrument for technical oral presentation skills evaluation: A case study in English technical communication course

    Science.gov (United States)

    Mohamed, Abdullah-Adnan; Asmawi, Adelina; Hamid, Mohd Rashid Ab; Mustafa, Zainol bin

    2015-02-01

    This paper reports a pilot study of Context Evaluation using a self-developed questionnaire distributed among engineering undergraduates at a university under study. The study aims to validate the self-developed questionnaires used in the Context evaluation, a component in the CIPP Model. The Context evaluation assesses background information for needs, assets, problems and opportunities relevant to beneficiaries of the study in a defined environment. Through the questionnaire, background information for the assessment of needs, assets and problems related to the engineering undergraduates' perceptions on the teaching and learning of technical oral presentation skills was collected and analysed. The questionnaire was developed using 5-points Likert scale to measure the constructs under study. They were distributed to 100 respondents with 79 returned. The respondents consisted of engineering undergraduates studied at various faculties at one technical university in Malaysia. The descriptive analysis of data for each item which makes up the construct for Context evaluation is found to be high. This implied that engineering undergraduates showed high interest in teaching and learning of technical oral presentation skills, thus their needs are met. Also, they agreed that assets and facilities are conducive to their learning. In conclusion, the context evaluation involving needs and assets factors are both considerably important; their needs are met and the assets and facilities do support their technical oral presentation skills learning experience.

  2. 76 FR 60478 - Hydrogen and Fuel Cell Technical Advisory Committee (HTAC)

    Science.gov (United States)

    2011-09-29

    ... Hydrogen and Fuel Cell Technical Advisory Committee (HTAC) AGENCY: Department of Energy, Office of Energy Efficiency and Renewable Energy. ACTION: Notice of open meeting. SUMMARY: The Hydrogen and Fuel Cell... Agenda: (Subject to change; updates will be posted on the website at: http://hydrogen.energy.gov...

  3. 75 FR 59705 - Hydrogen and Fuel Cell Technical Advisory Committee (HTAC)

    Science.gov (United States)

    2010-09-28

    ... Hydrogen and Fuel Cell Technical Advisory Committee (HTAC) AGENCY: Department of Energy, Office of Energy Efficiency and Renewable Energy. ACTION: Notice of open meeting. SUMMARY: The Hydrogen and Fuel Cell... will be posted on the web at http://hydrogen.energy.gov and copies of the final agenda will...

  4. Technical and economic analysis of using biomass energy

    Directory of Open Access Journals (Sweden)

    Piaskowska-Silarska Małgorzata

    2017-01-01

    Full Text Available In the first part of the article were presented the technical possibilities of obtaining solid biomass, biogas, landfill gas, a biogas from wastewater treatment plants, bioethanol and biodiesel. Then processes was described, allowing use of energy from biomass. As first was discussed the incineration which includes drying and degassing of the wood materials, wood gas burning at 1200°C, post-combustion gas and heat transfer in the heat exchanger. Then had been described gasification, or thermochemical conversion process, occurring at high temperature. It is two-stage process. In the first chamber at deficiency of air and at relatively low temperatures (450–800°C, the fuel is being degasified, resulting in creating combustible gas and a mineral residue (charcoal. In the second stage, secondary combustion chamber and at a temperature of about 1000–1200°C and in the presence of excess of oxygen resultant gas is burned. A further process is pyrolysis. It consists of the steps of drying fuel to a moisture level below 10%, milling the biomass into very small particles, the pyrolysis reaction, separation of solid products, cooling and collecting bio-oil. Then discusses co-generation, which is combined production of heat and electricity. In this situation where the biomass contains too much water it can be used for energy purposes through biochemical processes. The alcoholic fermentation results in decomposition of carbohydrates taking place under anaerobic conditions, and the product is bioethanol. Another biochemical process used for the production of liquid biofuels is esterification of vegetable oils. Methane fermentation in turn causes a decomposition of macromolecular organic substances with limited oxygen available. As a result, we obtain alcohols, lower organic acids, methane, carbon dioxide and water. There was analysis of economic increasing of solid biomass energy, biogas and liquid biofuels in the following article.

  5. Are chartists artists? The determinants and profitability of recommendations based on technical analysis

    NARCIS (Netherlands)

    Gerritsen, Dirk F.

    2016-01-01

    The value of technical analysis (TA) has been debated for decades; however, limited evidence exists on the profitability of investment recommendations issued by technical analysts. These ‘chartists’ sometimes claim that TA is an art rather than a science. We evaluated > 5000 TA-based buy and sell re

  6. Combining technical and organisational elements in layer of protection analysis

    NARCIS (Netherlands)

    Boers, M.N.; Gort, J.; Versloot, N.H.A.

    2005-01-01

    Traditionally, in process industry, risks are reduced with technical solutions and organisational measures. The performance of both types of "solutions" depends on many factors and can not easily be compared. Especially the effectiveness of organisational measures such as the use of procedures, educ

  7. Regulation of cell division in higher plants. Final technical report

    Energy Technology Data Exchange (ETDEWEB)

    Jacobs, Thomas W.

    2000-02-29

    Research in the latter part of the grant period was divided into two parts: (1) expansion of the macromolecular tool kit for studying plant cell division; (2) experiments in which the roles played by plant cell cycle regulators were to be cast in the light of the emerging yeast and animal cell paradigm for molecular control of the mitotic cycle. The first objectives were accomplished to a very satisfactory degree. With regard to the second part of the project, we were driven to change our objectives for two reasons. First, the families of cell cycle control genes that we cloned encoded such closely related members that the prospects for success at raising distinguishing antisera against each were sufficiently dubious as to be impractical. Epitope tagging is not feasible in Pisum sativum, our experimental system, as this species is not realistically transformable. Therefore, differentiating the roles of diverse cyclins and cyclin-dependent kinases was problematic. Secondly, our procedure for generating mitotically synchronized pea root meristems for biochemical studies was far too labor intensive for the proposed experiments. We therefore shifted our objectives to identifying connections between the conserved proteins of the cell cycle engine and factors that interface it with plant physiology and development. In this, we have obtained some very exciting results.

  8. 78 FR 27235 - Technical Guidance for Assessing Environmental Justice in Regulatory Analysis

    Science.gov (United States)

    2013-05-09

    ..., Office of Policy, National Center for Environmental Economics, Mail code 1809T, Environmental Protection... From the Federal Register Online via the Government Publishing Office ENVIRONMENTAL PROTECTION AGENCY Technical Guidance for Assessing Environmental Justice in Regulatory Analysis...

  9. U.S. Renewable Energy Technical Potentials. A GIS-Based Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Lopez, Anthony [National Renewable Energy Lab. (NREL), Golden, CO (United States); Roberts, Billy [National Renewable Energy Lab. (NREL), Golden, CO (United States); Heimiller, Donna [National Renewable Energy Lab. (NREL), Golden, CO (United States); Blair, Nate [National Renewable Energy Lab. (NREL), Golden, CO (United States); Porro, Gian [National Renewable Energy Lab. (NREL), Golden, CO (United States)

    2012-07-01

    This report presents the state-level results of a spatial analysis effort calculating energy technical potential, reported in square kilometers of available land, megawatts of capacity, and gigawatt-hours of generation, for six different renewable technologies. For this analysis, the system specific power density (or equivalent), efficiency (capacity factor), and land-use constraints were identified for each technology using independent research, published research, and professional contacts. This report also presents technical potential findings from previous reports.

  10. U.S. Renewable Energy Technical Potentials: A GIS-Based Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Lopez, A.; Roberts, B.; Heimiller, D.; Blair, N.; Porro, G.

    2012-07-01

    This report presents the state-level results of a spatial analysis effort calculating energy technical potential, reported in square kilometers of available land, megawatts of capacity, and gigawatt-hours of generation, for six different renewable technologies. For this analysis, the system specific power density (or equivalent), efficiency (capacity factor), and land-use constraints were identified for each technology using independent research, published research, and professional contacts. This report also presents technical potential findings from previous reports.

  11. Towards MOOC for Technical Courses: A Blended Learning Empirical Analysis

    OpenAIRE

    Siti Feirusz Ahmad Fesol

    2016-01-01

    Massive Open Online Learning (MOOC) is one of the rapidly growing and the most trending online learning platform throughout the world. As reported by Class Central up until December 2015, there are more than a total of 4200 courses, which enrolled more than 35 million students and adopted by more than 500 universities all over the world. Thus, the objective of this study is to identify the students’ readiness towards MOOC technical courses based on blended learning approach. This study adapte...

  12. Technical change in US industry: A cross-industry analysis

    Science.gov (United States)

    Nelson, R. R. (Editor)

    1981-01-01

    The nature of the public policies which have influenced the pace and pattern of technical progress in a number of American industries is studied with the view of assessing the broad effects of these policies. The industries studied are agriculture, pharmaceuticals, semiconductors, computers, civil aircraft, automobiles and residential construction. The policies considered include research and development funding as well as government procurement, education, information dissemination, patent protection, licensing, regulations, and anti-trust policies.

  13. Technical evaluation of Solar Cells, Inc., CdTe module and array at NREL

    Energy Technology Data Exchange (ETDEWEB)

    Kroposki, B.; Strand, T.; Hansen, R. [National Renewable Energy Lab., Golden, CO (United States); Powell, R.; Sasala, R. [Solar Cells, Inc., Toledo, OH (United States)

    1996-05-01

    The Engineering and Technology Validation Team at the National Renewable Energy Laboratory (NREL) conducts in-situ technical evaluations of polycrystalline thin-film photovoltaic (PV) modules and arrays. This paper focuses on the technical evaluation of Solar Cells, Inc., (SCI) cadmium telluride (CdTe) module and array performance by attempting to correlate individual module and array performance. This is done by examining the performance and stability of the modules and array over a period of more than one year. Temperature coefficients for module and array parameters (P{sub max}, V{sub oc}, V{sub max}, I{sub sc}, I{sub max}) are also calculated.

  14. UN Global Technical Regulation for Hydrogen Fuel Cell Vehicles

    Institute of Scientific and Technical Information of China (English)

    He Yuntang; Zheng Jinyang; Ou Kesheng

    2012-01-01

    1.Background Along with the gradual depletion of fossil energy such as petrol,coal and natural gas and the increasing attention paid to the environment problems,hydrogen energy as the important secondary energy source is developing at amazing pace.Since 1990s,the research on the application of hydrogen energy in transport has been increasingly vigorous,which is now one of the most active research areas for hydrogen energy.With the advantages of high energy conversion rate,good economical efficiency of the fuel,zero emission,etc.,hydrogen fuel cell vehicles have attracted the close concern and support from many countries,enterprises and academies.In recent years,our HFCV technologies have experienced rapid development,with joint efforts of production,teaching and research and continuous enhancement of international cooperation.

  15. Patent analysis for competitive technical intelligence and innovative thinking

    Directory of Open Access Journals (Sweden)

    H Dou

    2006-01-01

    Full Text Available Patents are a very useful source of technical information. The public availability of patents over the Internet, with for some databases (eg. Espacenet the assurance of a constant format, allows the development of high value added products using this information source and provides an easy way to analyze patent information. This simple and powerful tool facilitates the use of patents in academic research, in SMEs and in developing countries providing a way to use patents as a ideas resource thus improving technological innovation.

  16. Technical information report: Plasma melter operation, reliability, and maintenance analysis

    Energy Technology Data Exchange (ETDEWEB)

    Hendrickson, D.W. [ed.

    1995-03-14

    This document provides a technical report of operability, reliability, and maintenance of a plasma melter for low-level waste vitrification, in support of the Hanford Tank Waste Remediation System (TWRS) Low-Level Waste (LLW) Vitrification Program. A process description is provided that minimizes maintenance and downtime and includes material and energy balances, equipment sizes and arrangement, startup/operation/maintence/shutdown cycle descriptions, and basis for scale-up to a 200 metric ton/day production facility. Operational requirements are provided including utilities, feeds, labor, and maintenance. Equipment reliability estimates and maintenance requirements are provided which includes a list of failure modes, responses, and consequences.

  17. CIBS Solar Cell Development Final Scientific/Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Exstrom, Christopher L.; Soukup, Rodney J.; Ianno, Natale J.

    2011-09-28

    Efforts to fabricate and study a new photovoltaic material, copper indium boron diselenide (CuInxB1-xSe2 or CIBS), were undertaken. Attempts to prepare CIBS using sputtering deposition techniques resulted in segregation of boron from the rest of elements in the material. CIBS nanocrystals were prepared from the reaction of elemental Se with CuCl, InCl3, and boric acid in solution, but the product material quickly decomposed upon heating that was required in attempts to convert the nanocrystals into a thin film. The investigation of the reasons for the lack of CIBS material stability led to new structure-property studies of closely-related photovoltaic systems as well as studies of new solar cell materials and processing methods that could enhance the development of next-generation solar technologies. A detailed compositional study of CuIn1-xAlxSe2 (CIAS, a system closely related to CIBS) revealed a non-linear correlation between crystal lattice size and the Al/(In+Al) ratios with dual-phase formation being observed. A new nanocrystal-to-thin-film processing method was developed for the preparation of CuIn1-xGaxSe2 (CIGS) thin films in which colloidal Se particles are sprayed in contact with CuIn1-xGaxS2 nanoparticles and heated in an argon atmosphere with no other Se source in the system. The process is non-vacuum and does not require toxic gases such as Se vapor or H2Se. Expertise gained from these studies was applied to new research in the preparation of thin-film pyrite FeS2, an attractive earth-abundant candidate material for next-generation photovoltaics. Three methods successfully produced pure pyrite FeS2 films: sulfurization of sputtered Fe films, chemical bath deposition, and sulfurization of Fe2O3 sol-gel precursors. The last method produced pinhole-free films that may be viable for device development. Nickel, platinum, and possibly carbon would appear to serve as good ohmic contact materials. While CdS has a reasonable conduction band energy match to

  18. Microfluidics for single cell analysis

    DEFF Research Database (Denmark)

    Jensen, Marie Pødenphant

    Isolation and manipulation of single cells have gained an increasing interest from researchers because of the heterogeneity of cells from the same cell culture. Single cell analysis can ensure a better understanding of differences between individual cells and potentially solve a variety of clinic...

  19. Evaluating Technical Efficiency of Nursing Care Using Data Envelopment Analysis and Multilevel Modeling.

    Science.gov (United States)

    Min, Ari; Park, Chang Gi; Scott, Linda D

    2016-05-23

    Data envelopment analysis (DEA) is an advantageous non-parametric technique for evaluating relative efficiency of performance. This article describes use of DEA to estimate technical efficiency of nursing care and demonstrates the benefits of using multilevel modeling to identify characteristics of efficient facilities in the second stage of analysis. Data were drawn from LTCFocUS.org, a secondary database including nursing home data from the Online Survey Certification and Reporting System and Minimum Data Set. In this example, 2,267 non-hospital-based nursing homes were evaluated. Use of DEA with nurse staffing levels as inputs and quality of care as outputs allowed estimation of the relative technical efficiency of nursing care in these facilities. In the second stage, multilevel modeling was applied to identify organizational factors contributing to technical efficiency. Use of multilevel modeling avoided biased estimation of findings for nested data and provided comprehensive information on differences in technical efficiency among counties and states.

  20. Final Technical Report: Residential Fuel Cell Demonstration by the Delaware County Electric Cooperative, Inc.

    Energy Technology Data Exchange (ETDEWEB)

    Mark Hilson Schneider

    2007-06-06

    This demonstration project contributes to the knowledge base in the area of fuel cells in stationary applications, propane fuel cells, edge-of-grid applications for fuel cells, and energy storage in combination with fuel cells. The project demonstrated that it is technically feasible to meet the whole-house electrical energy needs of a typical upstate New York residence with a 5-kW fuel cell in combination with in-home energy storage without any major modifications to the residence or modifications to the consumption patterns of the residents of the home. The use of a fuel cell at constant output power through a 120-Volt inverter leads to system performance issues including: • relatively poor power quality as quantified by the IEEE-defined short term flicker parameter • relatively low overall system efficiency Each of these issues is discussed in detail in the text of this report. The fuel cell performed well over the 1-year demonstration period in terms of availability and efficiency of conversion from chemical energy (propane) to electrical energy at the fuel cell output terminals. Another strength of fuel cell performance in the demonstration was the low requirements for maintenance and repair on the fuel cell. The project uncovered a new and important installation consideration for propane fuel cells. Alcohol added to new propane storage tanks is preferentially absorbed on the surface of some fuel cell reformer desulfurization filters. The experience on this project indicates that special attention must be paid to the volume and composition of propane tank additives. Size, composition, and replacement schedules for the de-sulfurization filter bed should be adjusted to account for propane tank additives to avoid sulfur poisoning of fuel cell stacks. Despite good overall technical performance of the fuel cell and the whole energy system, the demonstration showed that such a system is not economically feasible as compared to other commercially available

  1. Technical Considerations for the Generation of Adoptively Transferred T Cells in Cancer Immunotherapy

    Directory of Open Access Journals (Sweden)

    Anthony Visioni

    2016-09-01

    Full Text Available A significant function of the immune system is the surveillance and elimination of aberrant cells that give rise to cancer. Even when tumors are well established and metastatic, immune-mediated spontaneous regressions have been documented. While there are have been various forms of immunotherapy, one of the most widely studied for almost 40 years is adoptive cellular immunotherapy, but its success has yet to be fully realized. Adoptive cell transfer (ACT is a therapeutic modality that has intrigued physicians and researchers for its many theoretical benefits. Preclinical investigations and human trials have utilized natural killer (NK cells, dendritic cells (DC, macrophages, T-cells or B-cells for ACT with the most intense research focused on T-cell ACT. T-cells are exquisitely specific to the target of its T-cell receptor (TCR, thus potentially reducing the amount of collateral damage and off-target effects from treatment. T-cells also possess a memory subset that may reduce the risk of recurrence of a cancer after the successful treatment of the primary disease. There are several options for the source of T-cells used in the generation of cells for ACT. Perhaps the most widely known source is T-cells generated from tumor-infiltrating lymphocytes (TILs. However, studies have also employed peripheral blood mononuclear cells (PBMCs, lymph nodes, and even induced pluripotent stem cells (IPSCs as a source of T-cells. Several important technical considerations exist regarding benefits and limitations of each source of T-cells. Unique aspects of T-cells factor into their ability to be efficacious in ACT including the total number of cells available for ACT, the anti-tumor efficacy on a per cell basis, the repertoire of TCRs specific to tumor cells, and their ability to traffic to various organs that harbor tumor. Current research is attempting to unlock the full potential of these cells to effectively and safely treat cancer.

  2. CellTracks cell analysis system for rare cell detection

    NARCIS (Netherlands)

    Kagan, Michael T.; Trainer, Michael N.; Bendele, Teresa; Rao, Chandra; Horton, Allen; Tibbe, Arjan G.; Greve, Jan; Terstappen, Leon W.M.M.

    2002-01-01

    The CellTracks system is a Compact Disk-based cell analyzer that, similar to flow cytometry, differentiates cells that are aligned while passing through focused laser beams. In CellTracks, only immuno-magnetically labeled cells are aligned and remain in position for further analysis. This feature is

  3. SRC-II slurry preheater technical uncertainties. Report for the technical data analysis program

    Energy Technology Data Exchange (ETDEWEB)

    1984-06-01

    This report reviews the performance, and draws conclusions therefrom, the coal slurry preheaters of the Ft. Lewis, Washington, Solvent Refined Coal (SRC) Pilot Plant in the following areas: Coking, Erosion Corrosion, Heat transfer and pressure drop effects. Using prudent engineering judgement it postulates how such conclusions should affect the design and operability of large preheaters in future commercial scale plants. Also a recommendation is made for a small scale research and development effort that should result in a much firmer preheater design for any future facility. This report should be read in conjunction with the Solvent Refined Coal (SRC) Final Report, and volumes 1 and 2 of Slurry Preheater Design, SRC-II Process and also Ft. Lewis Slurry Preheater Data Analysis, 1-1/2 Inch Coil by Gulf Science and Technology Company of Pittsburgh, Pennsylvania. The Pittsburg and Midway Coal Mining Co.'s background is based primarily on a racetrack shaped up-flow coil and these comments pertain specifically to a commercial heater of that type of design. 5 references, 12 figures, 1 table.

  4. The Profitability of the Strategy Linking Fundamental, Portfolio and Technical Analysis on the Polish Capital Market

    Directory of Open Access Journals (Sweden)

    Flotyński Marcin

    2016-12-01

    Full Text Available In the article, several methods of taking investment decisions are described: a fundamental, portfolio, and technical analysis. They constitute different approaches which are convenient for different types of investors with various expectations and time horizons of their investments. The simultaneous combination of these three analyses is not popular. The aim of this study is to test the effectiveness of simultaneous use of a fundamental analysis, portfolio analysis, and technical analysis for shares quoted on the Warsaw Stock Exchange (WSE in 2000–2007. The research hypothesis is advanced that the concurrent-linked application of a fundamental, portfolio, and technical analysis brings better results than the separate use of these analyses. Models of capital market, such as CAPM and APT, have been used, as well as P/E ratio, Return on Equity (RoE, Relative Strength Index (RSI, and Exponential Moving Average (EMA. The combination of a financial analysis, technical indicators, and models of the capital market in order to invest on the stock exchange is author’s own method. In general, the survey has been carried out on the grounds of quantitative methods (financial analysis, regression model, and multi regression model and a comparative analysis. The results of the research have been used to create diversified portfolios on the WSE. It occurs that the concurrent use of the three analyses brings the highest rate of return of a portfolio.

  5. Analysis of occupational accidents: prevention through the use of additional technical safety measures for machinery.

    Science.gov (United States)

    Dźwiarek, Marek; Latała, Agata

    2016-01-01

    This article presents an analysis of results of 1035 serious and 341 minor accidents recorded by Poland's National Labour Inspectorate (PIP) in 2005-2011, in view of their prevention by means of additional safety measures applied by machinery users. Since the analysis aimed at formulating principles for the application of technical safety measures, the analysed accidents should bear additional attributes: the type of machine operation, technical safety measures and the type of events causing injuries. The analysis proved that the executed tasks and injury-causing events were closely connected and there was a relation between casualty events and technical safety measures. In the case of tasks consisting of manual feeding and collecting materials, the injuries usually occur because of the rotating motion of tools or crushing due to a closing motion. Numerous accidents also happened in the course of supporting actions, like removing pollutants, correcting material position, cleaning, etc.

  6. Hydrogen as a fuel for fuel cell vehicles: A technical and economic comparison

    Energy Technology Data Exchange (ETDEWEB)

    Ogden, J.; Steinbugler, M.; Kreutz, T. [Princeton Univ., NJ (United States). Center for Energy and Environmental Studies

    1997-12-31

    All fuel cells currently being developed for near term use in vehicles require hydrogen as a fuel. Hydrogen can be stored directly or produced onboard the vehicle by reforming methanol, ethanol or hydrocarbon fuels derived from crude oil (e.g., Diesel, gasoline or middle distillates). The vehicle design is simpler with direct hydrogen storage, but requires developing a more complex refueling infrastructure. In this paper, the authors compare three leading options for fuel storage onboard fuel cell vehicles: compressed gas hydrogen storage; onboard steam reforming of methanol; onboard partial oxidation (POX) of hydrocarbon fuels derived from crude oil. Equilibrium, kinetic and heat integrated system (ASPEN) models have been developed to estimate the performance of onboard steam reforming and POX fuel processors. These results have been incorporated into a fuel cell vehicle model, allowing us to compare the vehicle performance, fuel economy, weight, and cost for various fuel storage choices and driving cycles. A range of technical and economic parameters were considered. The infrastructure requirements are also compared for gaseous hydrogen, methanol and hydrocarbon fuels from crude oil, including the added costs of fuel production, storage, distribution and refueling stations. Considering both vehicle and infrastructure issues, the authors compare hydrogen to other fuel cell vehicle fuels. Technical and economic goals for fuel cell vehicle and hydrogen technologies are discussed. Potential roles for hydrogen in the commercialization of fuel cell vehicles are sketched.

  7. Optimization of genetic analysis for single cell

    Directory of Open Access Journals (Sweden)

    hussein mouawia

    2012-03-01

    Full Text Available The molecular genetic analysis of microdissected cells by laser, a method for selecting a starting material of pure DNA or RNA uncontaminated. Our study focuses on technical pre-PCR (polymerase chain reaction for the amplification of DNA from a single cell (leukocyte isolated from human blood after laser microdissection and aims to optimize the yield of DNA extracted of this cell to be amplified without errors and provide reliable genetic analyzes. This study has allowed us to reduce the duration of cell lysis in order to perform the step of expanding genomic PEP (primer extension preamplification directly after lysis the same day and the quality of genomic amplification and eliminate purification step of the product PEP, step with a risk of contamination and risk of loss of genetic material related to manipulation. This approach has shown that the combination of at least 3 STR (short tandem repeat markers for genetic analysis of single cell improves the efficiency and accuracy of PCR and minimizes the loss of allele (allele drop out; ADO. This protocol can be applied to large scale and an effective means suitable for genetic testing for molecular diagnostic from isolated single cell (cancerous - fetal.

  8. Technical and tactical analysis of youth taekwondo performance.

    Science.gov (United States)

    Casolino, Erika; Lupo, Corrado; Cortis, Cristina; Chiodo, Salvatore; Minganti, Carlo; Capranica, Laura; Tessitore, Antonio

    2012-06-01

    This study aimed to analyze the technical and tactical aspects of young athletes during official taekwondo competitions. Fifty-nine youth taekwondo athletes (43 boys and 16 girls; age range: 10-12 years; weight category range: 59 kg) with at least 2 years of taekwondo training consisting of three 90-minute training sessions for 3 d·wk⁻¹ participated in this study. Thirty-seven matches (three 1-minute rounds, with 1-minute rest in between) were analyzed to verify the differences (p ≤ 0.05) in offensive and defensive actions in relation to gender (male, female), match outcome (winners, nonwinners), kicking leg (front, rear), and round (first, second, third). No difference emerged for gender and match outcome. With respect to defensive actions (8.4 ± 12.0%), youth athletes engaged more frequently (p < 0.0001) in offensive actions (91.6 ± 12.0%), which showed a significant decrease (p < 0.016) from the first round (42.3 ± 21.8%) to the second (33.1 ± 14.8%) and third (24.5 ± 16.0%) ones. Kicks performed with the rear leg (94.4 ± 7.8%) occurred more frequently (p < 0.0001) than those performed with the front leg (5.6 ± 7.8%). In considering that a high level of coordination is required to perform front-leg kicks and defensive actions necessitate a high level of tactical skills, these findings might indicate a not-yet complete attainment of fundamental coordinative capabilities in 10- to 12-year-old athletes, independently of match outcome. To enhance coordination capabilities in youth athletes, coaches are recommended to structure their training including skill-ability and sport-ability drills.

  9. Single Cell Isolation and Analysis

    Directory of Open Access Journals (Sweden)

    Ping Hu

    2016-10-01

    Full Text Available Increasing evidence shows that the heterogeneity of individual cells within a genetically identical population can be critical to their peculiar function and fate. Conventional cell based assays mainly analysis the average responses from a population cells, while the difference within individual cells may often be masked. The cell size, RNA transcripts and protein expression level are quite different within individual cells and these variations are key point to answer the problems in cancer, neurobiology, stem cell biology, immunology and developmental biology. To better understand the cell-to-cell variations, the single cell analysis can provide much more detailed information which may be helpful for therapeutic decisions in an increasingly personalized medicine. In this review, we will focus on the recent development in single cell analysis, including methods used in single cell isolation, analysis and some application examples. The review provides the historical background to single cell analysis, discusses limitations, and current and future possibilities in this exciting field of research.

  10. STEPS IN MAKING TECHNICAL ANALYSIS ECONOMIC ACTIVITY IN FARM

    OpenAIRE

    Teodora IVASCU

    2012-01-01

    Business analysis of agricultural units, research, information system based on the results obtained in their work, factors which have determined ways to improve them. It has an important role in ensuring the scientific management of farms, are an essential instrument in the exercise of leadership attributes on all hierarchical levels. In the analysis of business units in agriculture must take into consideration the peculiarities of agricultural production. These are reflected in the structure...

  11. Molten metal analysis by laser produced plasmas. Technical progress report

    Energy Technology Data Exchange (ETDEWEB)

    Kim, Yong W.

    1994-02-01

    A new method of molten metal analysis, based on time- and space-resolved spectroscopy of a laser-produced plasma (LPP) plume of a molten metal surface, has been implemented in the form of a prototype LPP sensor-probe, allowing in-situ analysis in less than 1 minute. The research at Lehigh University has been structured in 3 phases: laboratory verification of concept, comparison of LPP method with conventional analysis of solid specimens and field trials of prototype sensor-probe in small-scale metal shops, and design/production/installation of two sensor-probes in metal production shops. Accomplishments in the first 2 phases are reported. 6 tabs, 3 figs.

  12. Technical quality assessment of an optoelectronic system for movement analysis

    Science.gov (United States)

    Di Marco, R.; Rossi, S.; Patanè, F.; Cappa, P.

    2015-02-01

    The Optoelectronic Systems (OS) are largely used in gait analysis to evaluate the motor performances of healthy subjects and patients. The accuracy of marker trajectories reconstruction depends on several aspects: the number of cameras, the dimension and position of the calibration volume, and the chosen calibration procedure. In this paper we propose a methodology to evaluate the effects of the mentioned sources of error on the reconstruction of marker trajectories. The novel contribution of the present work consists in the dimension of the tested calibration volumes, which is comparable with the ones normally used in gait analysis; in addition, to simulate trajectories during clinical gait analysis, we provide non-default paths for markers as inputs. Several calibration procedures are implemented and the same trial is processed with each calibration file, also considering different cameras configurations. The RMSEs between the measured trajectories and the optimal ones are calculated for each comparison. To investigate the significant differences between the computed indices, an ANOVA analysis is implemented. The RMSE is sensible to the variations of the considered calibration volume and the camera configurations and it is always inferior to 43 mm.

  13. Building systems technical condition assessment based on the multilevel probabilistic analysis (rus

    Directory of Open Access Journals (Sweden)

    Sokolov V.A.

    2011-11-01

    Full Text Available Methods based on the stochastic apparatus technical diagnostics are put forward to solve problems concerning determination of the structural elements technical condition. Diagnosis is performed using probabilistic methods of recognition of technical conditions of complex technical systems. The diagnosis is conducted with the help of probabilistic methods of the complex engineering systems condition recognition based on the Bayesian analysis. The aforementioned approach and information theory methods are applied to run the multilevel diagnostics of elements and systems of floor slabs in old urban buildings. Multilevel diagnostics was done in the concepts of information entropy. During the analysis of the results of forward and reverse traces on the hierarchical diagnostics structure the next conclusion was made. The forward trace allows to determine the technical condition and category for the each element of each level in the hierarchical structure and the reverse trace gives a possibility to evaluate the contribution of each element condition to the information about the whole building condition. The last point can be an argumentative basis for the account of physical depreciation of building structures.

  14. Technical- and Environmental-Efficiency Analysis of Irrigated Cotton-Cropping Systems in Punjab, Pakistan Using Data Envelopment Analysis

    Science.gov (United States)

    Ullah, Asmat; Perret, Sylvain R.

    2014-08-01

    Cotton cropping in Pakistan uses substantial quantities of resources and adversely affects the environment with pollutants from the inputs, particularly pesticides. A question remains regarding to what extent the reduction of such environmental impact is possible without compromising the farmers' income. This paper investigates the environmental, technical, and economic performances of selected irrigated cotton-cropping systems in Punjab to quantify the sustainability of cotton farming and reveal options for improvement. Using mostly primary data, our study quantifies the technical, cost, and environmental efficiencies of different farm sizes. A set of indicators has been computed to reflect these three domains of efficiency using the data envelopment analysis technique. The results indicate that farmers are broadly environmentally inefficient; which primarily results from poor technical inefficiency. Based on an improved input mix, the average potential environmental impact reduction for small, medium, and large farms is 9, 13, and 11 %, respectively, without compromising the economic return. Moreover, the differences in technical, cost, and environmental efficiencies between small and medium and small and large farm sizes were statistically significant. The second-stage regression analysis identifies that the entire farm size significantly affects the efficiencies, whereas exposure to extension and training has positive effects, and the sowing methods significantly affect the technical and environmental efficiencies. Paradoxically, the formal education level is determined to affect the efficiencies negatively. This paper discusses policy interventions that can improve the technical efficiency to ultimately increase the environmental efficiency and reduce the farmers' operating costs.

  15. Quantitative Indicators for Defense Analysis. Volume II. Technical Report

    Science.gov (United States)

    1975-06-01

    34*"WTOiw«* piB ^ r- ••’ ’ ’■’.WH""" - "«.JH QUAURANT II Hot War JIoL ]War land i Cold I |Criscs War iThreaten ed - Crisis 1...34The Political Analysis of Negotiations," World Politics 26. 3 (April). ^(1971) The Politics of Trade Negotiations Between Africa and the EEC

  16. Technical analysis of four archaeological andean painted textiles

    OpenAIRE

    2016-01-01

    This project investigates the materials and manufacturing techniques used to create four archaeological Andean painted textiles in the collection of the National Museum of the American Indian, Smithsonian Institution. The textiles are attributed to Peru but have minimal provenience. Building on previous work by other scholars on similar archaeological textiles, the materials and manufacturing techniques are identified and characterized by observation, documentation, and scientific analysis. S...

  17. Longitudinal Trend Analysis of Performance Indicators for South Carolina's Technical Colleges

    Science.gov (United States)

    Hossain, Mohammad Nurul

    2010-01-01

    This study included an analysis of the trend of performance indicators for the technical college sector of higher education in South Carolina. In response to demands for accountability and transparency in higher education, the state of South Carolina developed sector specific performance indicators to measure various educational outcomes for each…

  18. Technical Training in the MNCs in Malaysia: A Case Study Analysis of the Petrochemical Industry

    Science.gov (United States)

    Hooi, Lai Wan

    2010-01-01

    Purpose: The aim of this paper is to gain insight into some of the types of training and development practices that are carried out in the chemical industry for technical workers. A salient focus of the study is to make a comparative analysis of four MNCs, which were selected based on equity ownership, to ascertain whether T&D practices are…

  19. 10 CFR 52.79 - Contents of applications; technical information in final safety analysis report.

    Science.gov (United States)

    2010-01-01

    ... 10 Energy 2 2010-01-01 2010-01-01 false Contents of applications; technical information in final safety analysis report. 52.79 Section 52.79 Energy NUCLEAR REGULATORY COMMISSION (CONTINUED) LICENSES, CERTIFICATIONS, AND APPROVALS FOR NUCLEAR POWER PLANTS Combined Licenses § 52.79 Contents of...

  20. Meta-Analysis as a Choice to Improve Research in Career and Technical Education

    Science.gov (United States)

    Gordon, Howard R. D.; McClain, Clifford R.; Kim, Yeonsoo; Maldonado, Cecilia

    2010-01-01

    A search of the ERIC and Academic Search Premier data bases, and a comprehensive review of literature suggest that meta-analysis is ignored by career and technical education (CTE) researchers, a situation that is regrettable but remediable. The purpose of this theoretical paper is to provide CTE researchers and consumers with procedures for…

  1. An Application of Fuzzy Theory to Technical Competency Analysis for the Entry-Level Electronic Technician.

    Science.gov (United States)

    Chang, Liang-Te; And Others

    A study was conducted to develop the electronic technical competencies of duty and task analysis by using a revised DACUM (Developing a Curriculum) method, a questionnaire survey, and a fuzzy synthesis operation. The revised DACUM process relied on inviting electronics trade professionals to analyze electronic technology for entry-level…

  2. ENHANCED SEVERE TRANSIENT ANALYSIS FOR PREVENTION TECHNICAL PROGRAM PLAN

    Energy Technology Data Exchange (ETDEWEB)

    Gougar, Hans [Idaho National Laboratory

    2014-09-01

    This document outlines the development of a high fidelity, best estimate nuclear power plant severe transient simulation capability that will complement or enhance the integral system codes historically used for licensing and analysis of severe accidents. As with other tools in the Risk Informed Safety Margin Characterization (RISMC) Toolkit, the ultimate user of Enhanced Severe Transient Analysis and Prevention (ESTAP) capability is the plant decision-maker; the deliverable to that customer is a modern, simulation-based safety analysis capability, applicable to a much broader class of safety issues than is traditional Light Water Reactor (LWR) licensing analysis. Currently, the RISMC pathway’s major emphasis is placed on developing RELAP-7, a next-generation safety analysis code, and on showing how to use RELAP-7 to analyze margin from a modern point of view: that is, by characterizing margin in terms of the probabilistic spectra of the “loads” applied to systems, structures, and components (SSCs), and the “capacity” of those SSCs to resist those loads without failing. The first objective of the ESTAP task, and the focus of one task of this effort, is to augment RELAP-7 analyses with user-selected multi-dimensional, multi-phase models of specific plant components to simulate complex phenomena that may lead to, or exacerbate, severe transients and core damage. Such phenomena include: coolant crossflow between PWR assemblies during a severe reactivity transient, stratified single or two-phase coolant flow in primary coolant piping, inhomogeneous mixing of emergency coolant water or boric acid with hot primary coolant, and water hammer. These are well-documented phenomena associated with plant transients but that are generally not captured in system codes. They are, however, generally limited to specific components, structures, and operating conditions. The second ESTAP task is to similarly augment a severe (post-core damage) accident integral analyses code

  3. Technical Analysis on Mechanical Model Based Football Curveball

    Directory of Open Access Journals (Sweden)

    Feng Li

    2013-04-01

    Full Text Available In this study, from the angles the physics and biomechanics, in the case of the curveball generated by the rotation problem analysis and exposition, considering the speed, rotation, the wall, the goalkeeper, goaltender factors and football running track and the theory trajectory deviation factor, making the model as much as possible to simulate the actual effect and using the MATLAB software to draw the flight of the ball trajectory simulation. Reference designed for teaching, training and competition as well as to further deepen the awareness and understanding of football curveball. It can improve the free kick guidance and not only help the shooter to select the best shooting methods, but also for the goalkeeper has targeted to fighting with reference.

  4. Special Operations Forces (SOF) technical analysis and evaluation

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1987-08-31

    In response to Task Order 001, Los Alamos National Laboratory Contract 9-L5H-1508P-1, Betac Corporation is pleased to provide ten quick-response, short-term analytical papers in support of Low Intensity Conflict (LIC) and Special Operations (SO). The papers are study methodologies which provide background, baseline, concepts, approaches, and recommendations in the mission areas identified in the Statement of Work. Although the Statement of Work specifies only nine papers, a tenth paper has been included addressing Command Relationships, since this subject affects all other topics and is of critical importance to USCINCSOC in establishing the United States Special Operations Command (USSOCOM). Each paper addresses the feasibility of further effort in each area of interest. The ten papers address: (1) mission support systems; (2) research, development, and acquisition; (3) headquarters equipment; (4) C3I architecture; (5) intelligence dissemination; (6) intelligence collection management; (7) intelligence support to SOF targeting; (8) joint mission area analysis (JMAA); (9) joint SOF master plan; and (10) command relationships.

  5. Sources of Technical Variability in Quantitative LC-MS Proteomics: Human Brain Tissue Sample Analysis.

    Energy Technology Data Exchange (ETDEWEB)

    Piehowski, Paul D.; Petyuk, Vladislav A.; Orton, Daniel J.; Xie, Fang; Moore, Ronald J.; Ramirez Restrepo, Manuel; Engel, Anzhelika; Lieberman, Andrew P.; Albin, Roger L.; Camp, David G.; Smith, Richard D.; Myers, Amanda J.

    2013-05-03

    To design a robust quantitative proteomics study, an understanding of both the inherent heterogeneity of the biological samples being studied as well as the technical variability of the proteomics methods and platform is needed. Additionally, accurately identifying the technical steps associated with the largest variability would provide valuable information for the improvement and design of future processing pipelines. We present an experimental strategy that allows for a detailed examination of the variability of the quantitative LC-MS proteomics measurements. By replicating analyses at different stages of processing, various technical components can be estimated and their individual contribution to technical variability can be dissected. This design can be easily adapted to other quantitative proteomics pipelines. Herein, we applied this methodology to our label-free workflow for the processing of human brain tissue. For this application, the pipeline was divided into four critical components: Tissue dissection and homogenization (extraction), protein denaturation followed by trypsin digestion and SPE clean-up (digestion), short-term run-to-run instrumental response fluctuation (instrumental variance), and long-term drift of the quantitative response of the LC-MS/MS platform over the 2 week period of continuous analysis (instrumental stability). From this analysis, we found the following contributions to variability: extraction (72%) >> instrumental variance (16%) > instrumental stability (8.4%) > digestion (3.1%). Furthermore, the stability of the platform and its’ suitability for discovery proteomics studies is demonstrated.

  6. Technical Analysis of the Hydrogen Energy Station Concept, Phase I and Phase II

    Energy Technology Data Exchange (ETDEWEB)

    TIAX, LLC

    2005-05-04

    patterns would be most viable for an energy station, TIAX developed several criteria for selecting a representative set of technology configurations. TIAX applied these criteria to all possible technology configurations to determine an optimized set for further analysis, as shown in Table ES-1. This analysis also considered potential energy station operational scenarios and their impact upon hydrogen and power production. For example, an energy station with a 50-kWe reformer could generate enough hydrogen to serve up to 12 vehicles/day (at 5 kg/fill) or generate up to 1,200 kWh/day, as shown in Figure ES-1. Buildings that would be well suited for an energy station would utilize both the thermal and electrical output of the station. Optimizing the generation and utilization of thermal energy, hydrogen, and electricity requires a detailed look at the energy transfer within the energy station and the transfer between the station and nearby facilities. TIAX selected the Baseline configuration given in Table ES-1 for an initial analysis of the energy and mass transfer expected from an operating energy station. Phase II The purpose of this technical analysis was to analyze the development of a hydrogen-dispensing infrastructure for transportation applications through the installation of a 50-75 kW stationary fuel cell-based energy station at federal building sites. The various scenarios, costs, designs and impacts of such a station were quantified for a hypothetical cost-shared program that utilizes a natural gas reformer to provide hydrogen fuel for both the stack(s) and a limited number of fuel cell powered vehicles, with the possibility of using cogeneration to support the building heat load.

  7. Identification of the Key Fields and Their Key Technical Points of Oncology by Patent Analysis.

    Directory of Open Access Journals (Sweden)

    Ting Zhang

    Full Text Available This paper aims to identify the key fields and their key technical points of oncology by patent analysis.Patents of oncology applied from 2006 to 2012 were searched in the Thomson Innovation database. The key fields and their key technical points were determined by analyzing the Derwent Classification (DC and the International Patent Classification (IPC, respectively. Patent applications in the top ten DC occupied 80% of all the patent applications of oncology, which were the ten fields of oncology to be analyzed. The number of patent applications in these ten fields of oncology was standardized based on patent applications of oncology from 2006 to 2012. For each field, standardization was conducted separately for each of the seven years (2006-2012 and the mean of the seven standardized values was calculated to reflect the relative amount of patent applications in that field; meanwhile, regression analysis using time (year and the standardized values of patent applications in seven years (2006-2012 was conducted so as to evaluate the trend of patent applications in each field. Two-dimensional quadrant analysis, together with the professional knowledge of oncology, was taken into consideration in determining the key fields of oncology. The fields located in the quadrant with high relative amount or increasing trend of patent applications are identified as key ones. By using the same method, the key technical points in each key field were identified. Altogether 116,820 patents of oncology applied from 2006 to 2012 were retrieved, and four key fields with twenty-nine key technical points were identified, including "natural products and polymers" with nine key technical points, "fermentation industry" with twelve ones, "electrical medical equipment" with four ones, and "diagnosis, surgery" with four ones.The results of this study could provide guidance on the development direction of oncology, and also help researchers broaden innovative ideas and

  8. Analysis Results for ARRA Projects: Enabling Fuel Cell Market Transformation (Presentation)

    Energy Technology Data Exchange (ETDEWEB)

    Kurtz, J.; Wipke, K.; Sprik, S.; Ramsden, T.; Ainscough, C.; Saur, G.

    2012-06-01

    This presentation discusses analysis results for American Recovery and Reinvestment Act early market fuel cell deployments and describes the objective of the project and its relevance to the Department of Energy Hydrogen and Fuel Cells Program; NREL's analysis approach; technical accomplishments including publication of a fourth set of composite data products; and collaborations and future work.

  9. Hydrogen Monitoring Requirements in the Global Technical Regulation on Hydrogen and Fuel Cell Vehicles: Preprint

    Energy Technology Data Exchange (ETDEWEB)

    Buttner, William; Rivkin, Carl; Burgess, Robert; Hartmann, Kevin; Bubar, Max; Post, Matthew; Boon-Brett, Lois; Weidner, Eveline; Moretto, Pietro

    2016-07-01

    The United Nations Global Technical Regulation (GTR) Number 13 (Global Technical Regulation on Hydrogen and Fuel Cell Vehicles) is the defining document regulating safety requirements in hydrogen vehicles, and in particular fuel cell electric vehicles (FCEV). GTR Number 13 has been formally implemented and will serve as the basis for the national regulatory standards for FCEV safety in North America (Canada, United States), Japan, Korea, and the European Union. The GTR defines safety requirement for these vehicles, including specifications on the allowable hydrogen levels in vehicle enclosures during in-use and post-crash conditions and on the allowable hydrogen emissions levels in vehicle exhaust during certain modes of normal operation. However, in order to be incorporated into national regulations, that is, in order to be binding, methods to verify compliance to the specific requirements must exist. In a collaborative program, the Sensor Laboratories at the National Renewable Energy Laboratory in the United States and the Joint Research Centre, Institute for Energy and Transport in the Netherlands have been evaluating and developing analytical methods that can be used to verify compliance to the hydrogen release requirement as specified in the GTR.

  10. The technical analysis of the stock exchange and physics: Japanese candlesticks for solar activity

    Science.gov (United States)

    Dineva, C.; Atanasov, V.

    2013-09-01

    In this article, we use the Japanese candlesticks, a method popular in the technical analysis of the Stock/Forex markets and apply it to a variable in physics-the solar activity. This method is invented and used exclusively for economic analysis and its application to a physical problem produced unexpected results. We found that the Japanese candlesticks are convenient tool in the analysis of the variables in the physics of the Sun. Based on our observations, we differentiated a new cycle in the solar activity.

  11. TAPPS Release 1: Plugin-Extensible Platform for Technical Analysis and Applied Statistics

    Directory of Open Access Journals (Sweden)

    Justin Sam Chew

    2016-01-01

    Full Text Available We present the first release of TAPPS (Technical Analysis and Applied Statistics System; a Python implementation of a thin software platform aimed towards technical analyses and applied statistics. The core of TAPPS is a container for 2-dimensional data frame objects and a TAPPS command language. TAPPS language is not meant to be a programming language for script and plugin development but for the operational purposes. In this aspect, TAPPS language takes on the flavor of SQL rather than R, resulting in a shallower learning curve. All analytical functions are implemented as plugins. This results in a defined plugin system, which enables rapid development and incorporation of analysis functions. TAPPS Release 1 is released under GNU General Public License 3 for academic and non-commercial use. TAPPS code repository can be found at http://github.com/mauriceling/tapps.

  12. Technical and Feasibility Analysis of Gasoline and Natural Gas Fuelled Vehicles

    OpenAIRE

    Charalambos Chasos; George Karagiorgis; Chris Christodoulou

    2014-01-01

    There is recent interest for the utilisation of natural gas for empowering the internal combustion engines (ICE) of vehicles. The production of novel natural gas ICE for vehicles, as well as the conversion of existing gasoline fuelled ICE of vehicles to natural gas fuelled ICE are new technologies which require to be analysed and assessed. The objective of the present study is to examine the adaptation of natural gas as vehicle fuel and carry out a technical analysis and an economical feasibi...

  13. Electrical circuit analysis and technical english: articulation in a project-based learning environment

    OpenAIRE

    Oliveira, José Manuel; Branco, Denise; Oliveira, João Pedro Estima de

    2010-01-01

    This paper describes the integration of Electrical Circuit Analysis and Technical English in the same curricular module, in the context of a Project Based Learning (PBL) environment. The idea consists in taking advantage of the PBL setting to provide a context for the learning of the two different subjects in an articulated manner. The results of the evaluation of the first iteration of this experiment will also be discussed.

  14. Technical Performance Analysis of Iran Premier League Soccer Players in 2012-2013 Season

    Directory of Open Access Journals (Sweden)

    Mohsen Javani

    2015-10-01

    Full Text Available Background and purpose of study : analysis of IRAN premier league soccer players’ technical performance in season 2012-2013, using a computerized match analysis system (Borhan Mobin Development Management Co, IRAN. Material and methods: in this study, data were obtained from 120 players, who performed in competitions 90 minutes. The players were classified into 3 positional roles: defenders, midfielders and forwards. Technical performance variables analysis included: total passes, total successful passes, pass accuracy, total shots; total shots to target, shot accuracy, ball interception and ball losses. The data were statistically analyzed by one-way ANOVA, Kruskal-Wallis, Mann-Whitney U and Tukey post hoc test. Results : The findings of this study showed that players performed about 45 passes per competition. Midfielders and defenders had significantly higher number of passes than forwards. Pass accuracy was about 67% and there were no significant differences between positional roles. Also, the players performed about 0.8 shots per competition, forwards and midfielders had significantly higher number of shots than defenders. Shot accuracy was about 31%; midfielders and forwards had significantly higher shot accuracy than defenders. Forwards showed significantly lower ball interception and higher ball losses than other positions. Conclusion : The result of this study showed that there were significant differences between some technical actions in positional roles. Therefore, coaches can use this information for individualization of training according to playing positions and for optimization of training in the amateur game.

  15. Meta-analysis of the technical performance of an imaging procedure: guidelines and statistical methodology.

    Science.gov (United States)

    Huang, Erich P; Wang, Xiao-Feng; Choudhury, Kingshuk Roy; McShane, Lisa M; Gönen, Mithat; Ye, Jingjing; Buckler, Andrew J; Kinahan, Paul E; Reeves, Anthony P; Jackson, Edward F; Guimaraes, Alexander R; Zahlmann, Gudrun

    2015-02-01

    Medical imaging serves many roles in patient care and the drug approval process, including assessing treatment response and guiding treatment decisions. These roles often involve a quantitative imaging biomarker, an objectively measured characteristic of the underlying anatomic structure or biochemical process derived from medical images. Before a quantitative imaging biomarker is accepted for use in such roles, the imaging procedure to acquire it must undergo evaluation of its technical performance, which entails assessment of performance metrics such as repeatability and reproducibility of the quantitative imaging biomarker. Ideally, this evaluation will involve quantitative summaries of results from multiple studies to overcome limitations due to the typically small sample sizes of technical performance studies and/or to include a broader range of clinical settings and patient populations. This paper is a review of meta-analysis procedures for such an evaluation, including identification of suitable studies, statistical methodology to evaluate and summarize the performance metrics, and complete and transparent reporting of the results. This review addresses challenges typical of meta-analyses of technical performance, particularly small study sizes, which often causes violations of assumptions underlying standard meta-analysis techniques. Alternative approaches to address these difficulties are also presented; simulation studies indicate that they outperform standard techniques when some studies are small. The meta-analysis procedures presented are also applied to actual [18F]-fluorodeoxyglucose positron emission tomography (FDG-PET) test-retest repeatability data for illustrative purposes.

  16. Analysis and Evaluation of Technical Data on the Photochromic and Non-Linear Optical Properties of Materials

    Science.gov (United States)

    1990-06-25

    t ,, A FILE C’D AD-A2 23 855 Sixth Quarterly Technical Report Analysis and Evaluation of Technical Data on the Photochromic and Non-Linear Optical...the back surface of a transparent support and place an absorbing beam dump behind and at an angle to that of the reflective coating . In this

  17. Endovascular biopsy: Technical feasibility of novel endothelial cell harvesting devices assessed in a rabbit aneurysm model

    Science.gov (United States)

    Bauer, Diana; Sun, Zhengda; Stillson, Carol; Nelson, Jeffrey; Barry, David; Hetts, Steven W; Higashida, Randall T; Dowd, Christopher F; Halbach, Van V; Su, Hua; Saeed, Maythem M

    2015-01-01

    The lack of safe and reliable methods to sample vascular tissue in situ limits discovery of the underlying genetic and pathophysiological mechanisms of many vascular disorders, including aneurysms. We investigated the feasibility and comparable efficacy of in vivo vascular endothelial cell sampling using a spectrum of endovascular devices. Using the rabbit elastase carotid aneurysm model we evaluated the performance of existing aneurysmal coils, intracranial stents, and stent-like devices to collect vascular endothelial cells. Additionally, we modified a subset of devices to assess the effects of alterations to coil pitch, coil wire contour, and stent surface finishing. Device performance was evaluated by (1) the number of viable endothelial cells harvested, (2) the degree of vascular wall damage analyzed using digital subtraction angiography and histopathological analysis, and (3) the ease of device navigability and retrieval. Isolated cells underwent immunohistochemical analysis to confirm cell type and viability. Coil and stent specifications, technique, and endothelial cell counts were tabulated and statistical analysis performed. Using conventional detachable-type and modified aneurysm coils 11 of 14 (78.6%) harvested endothelial cells with a mean of 7.93 (±8.33) cells/coil, while 15 of 15 (100%) conventional stents, stent-like devices and modified stents harvested endothelial cells with a mean of 831.33 (±887.73) cells/device. Coil stiffness was significantly associated with endothelial cell count in univariate analysis (p = 0.044). For stents and stent-like devices univariate analysis demonstrated stent-to-aorta diameter ratios (p = 0.001), stent length (p = 0.049), and the use of a pulling retrieval technique (p = 0.019) significantly predictive of endothelial cell counts, though a multivariate model using these variables demonstrated only the stent-to-aorta diameter ratio (p = 0.029) predictive of endothelial cell counts. Modified

  18. Technical and Feasibility Analysis of Gasoline and Natural Gas Fuelled Vehicles

    Directory of Open Access Journals (Sweden)

    Charalambos Chasos

    2014-03-01

    Full Text Available There is recent interest for the utilisation of natural gas for empowering the internal combustion engines (ICE of vehicles. The production of novel natural gas ICE for vehicles, as well as the conversion of existing gasoline fuelled ICE of vehicles to natural gas fuelled ICE are new technologies which require to be analysed and assessed. The objective of the present study is to examine the adaptation of natural gas as vehicle fuel and carry out a technical analysis and an economical feasibility analysis of the two types of ICE vehicles, namely gasoline and natural gas fuelled vehicles. The technical model uses the physical properties of the two fuels and the performance factors of internal combustion engines including brake thermal efficiency. The resulting exhaust gas emissions are also estimated by the technical model using combustion calculations which provide the expected levels of exhaust gas emissions. Based on the analysis with the technical model, comparisons of the two types of engines are performed. Furthermore, the estimated performance characteristics of the two types of engines, along with local statistical data on annual fuel imports and annual fuel consumption for transportation and data on the vehicles fleet for the case study of Cyprus are used as input in the economical model. For the base year 2013, data of natural gas price is also used in the economical model. The economical model estimates the capital cost, the carbon dioxide emissions avoidance of fines, the net present value and the internal rate of return of the investment of large scale adaptation of natural gas fuelled vehicles for the case study. From the results and comparisons, conclusions are drawn and recommendations are provided for the adaptation of natural gas vehicles which can provide improved performance with reduced pollutant emissions.

  19. Minimal residual disease detection in mantle cell lymphoma: technical aspects and clinical relevance.

    Science.gov (United States)

    Pott, Christiane

    2011-07-01

    The prognostic impact of minimal residual disease (MRD) has been demonstrated for several hematologic malignancies. While in acute lymphoblastic leukemias MRD assessment by polymerase chain reaction (PCR)-based methods has been established as an important tool for clinical risk assessment and is part of clinical management, data demonstrating a prognostic value of MRD in mantle cell lymphoma (MCL) were sparse and results from randomized trials have been published only recently. In the present review technical aspects of different MRD detection methods are discussed, as well as the prognostic relevance of MRD in the context of clinical trials in patients with MCL. Furthermore, recommendations are given for workflow and useful implication of MRD in future clinical trials design.

  20. Technical and Scale Efficiency in Spanish Urban Transport: Estimating with Data Envelopment Analysis

    Directory of Open Access Journals (Sweden)

    I. M. García Sánchez

    2009-01-01

    Full Text Available The paper undertakes a comparative efficiency analysis of public bus transport in Spain using Data Envelopment Analysis. A procedure for efficiency evaluation was established with a view to estimating its technical and scale efficiency. Principal components analysis allowed us to reduce a large number of potential measures of supply- and demand-side and quality outputs in three statistical factors assumed in the analysis of the service. A statistical analysis (Tobit regression shows that efficiency levels are negative in relation to the population density and peak-to-base ratio. Nevertheless, efficiency levels are not related to the form of ownership (public versus private. The results obtained for Spanish public transport show that the average pure technical and scale efficiencies are situated at 94.91 and 52.02%, respectively. The excess of resources is around 6%, and the increase in accessibility of the service, one of the principal components summarizing the large number of output measures, is extremely important as a quality parameter in its performance.

  1. Comparative socio-technical discourse analysis of Water Sensitive Urban Design for Melbourne, Australia and Copenhagen, Denmark

    DEFF Research Database (Denmark)

    Mo Madsen, Herle; Brown, Rebekah; Elle, Morten

    This paper presents a comparative socio-technical discourse analysis of WUSD for Melbourne and Copenhagen. Despite Melbourne appearing to have progressed further, the technology pathways are very similar, suggesting significant potential for transfer of insights and policy mechanisms. However...

  2. Comparative socio-technical discourse analysis of Water Sensitive Urban Design for Melbourne, Australia and Copenhagen, Denmark

    DEFF Research Database (Denmark)

    Madsen, Herle Mo; Brown, R.; Elle, Morten

    2013-01-01

    This paper presents a comparative socio-technical discourse analysis of WUSD for Melbourne and Copenhagen. Despite Melbourne appearing to have progressed further, the technology pathways are very similar, suggesting significant potential for transfer of insights and policy mechanisms. However...

  3. IETM技术插图制作现状简析%Brief Analysis on Technical Illustration Procedure Instance for IETM

    Institute of Scientific and Technical Information of China (English)

    余容; 王峰; 吴家菊; 胥永康

    2015-01-01

    技术插图是交互式电子技术手册的重要组成部分,技术插图制作的优劣直接影响着交互式电子技术手册的整体交互性展现。为解决交互式电子技术手册技术插图制作过程中存在的诸多问题,对交互式电子技术手册标准和插图制作现状进行全面分析,提出合理的对策和建议,并预测未来发展前景。该分析可为有关部门和技术人员提供参考。%Technical illustration is the most important part of interactive electronic technical manual (IETM). The performance of technical illustration has an important effect on interactive electronic technical manual. To solve those problems during the implement procedure of IETM is that we need to try out of complicated technical illustration procedure and summarize current situation experience continuously. This paper is concerned with the technical illustration problems and rationality recommendation. This paper shows the tendency prediction of technical illustration for interactive electronic technical manual. This analysis can supply consultation for relevant portion and technical staff.

  4. Development of Droplet Microfluidics Enabling High-Throughput Single-Cell Analysis

    Directory of Open Access Journals (Sweden)

    Na Wen

    2016-07-01

    Full Text Available This article reviews recent developments in droplet microfluidics enabling high-throughput single-cell analysis. Five key aspects in this field are included in this review: (1 prototype demonstration of single-cell encapsulation in microfluidic droplets; (2 technical improvements of single-cell encapsulation in microfluidic droplets; (3 microfluidic droplets enabling single-cell proteomic analysis; (4 microfluidic droplets enabling single-cell genomic analysis; and (5 integrated microfluidic droplet systems enabling single-cell screening. We examine the advantages and limitations of each technique and discuss future research opportunities by focusing on key performances of throughput, multifunctionality, and absolute quantification.

  5. Aircraft/Air Traffic Management Functional Analysis Model: Technical Description. 2.0

    Science.gov (United States)

    Etheridge, Melvin; Plugge, Joana; Retina, Nusrat

    1998-01-01

    The Aircraft/Air Traffic Management Functional Analysis Model, Version 2.0 (FAM 2.0), is a discrete event simulation model designed to support analysis of alternative concepts in air traffic management and control. FAM 2.0 was developed by the Logistics Management Institute (LMI) under a National Aeronautics and Space Administration (NASA) contract. This document provides a technical description of FAM 2.0 and its computer files to enable the modeler and programmer to make enhancements or modifications to the model. Those interested in a guide for using the model in analysis should consult the companion document, Aircraft/Air Traffic Management Functional Analysis Model, Version 2.0 Users Manual.

  6. Global Spent Fuel Logistics Systems Study (GSFLS). Volume 3A. GSFLS technical analysis (appendix). Interim report

    Energy Technology Data Exchange (ETDEWEB)

    Kriger, A.

    1978-01-31

    This report is a part of the interim report documentation for the Global Spent Fuel Logistics System (GSFLS) study. The technical and financial considerations underlying a global spent fuel logistics systems have been studied and are reported. The Pacific Basin is used as a model throughout this report; however the stated methodology and, in many cases, considerations and conclusions are applicable to other global regions. Spent fuel discharge profiles for Pacific Basin Countries were used to determine the technical systems requirements for alternative concepts. Functional analyses and flows were generated to define both system design requirements and logistics parameters. A technology review was made to ascertain the state-of-the-art of relevant GSFLS technical systems. Modular GSFLS facility designs were developed using the information generated from the functional analysis and technology review. The modular facility designs were used as a basis for siting and cost estimates for various GSFLS alternatives. Various GSFLS concepts were analyzed from a financial and economic perspective in order to provide total concepts costs and ascertain financial and economic sensitivities to key GSFLS variations. Results of the study include quantification of GSFLS facility and hardware requirements; drawings of relevant GSFLS facility designs; system cost estimates; financial reports - including user service charges; and comparative analyses of various GSFLS alternatives.

  7. THE USE OF THE PATENT ANALYSIS METHOD FOR FINDING ANALOGUES AND PROTOTYPES OF RECEIVED TECHNICAL SOLUTIONS

    Directory of Open Access Journals (Sweden)

    Irina Petrova

    2016-03-01

    Full Text Available The research deals with the issue of the patent analysis efficiency, which is a necessary stage of seaching analogues and prototypes to obtain technical solutions. The article presents the results of analyzing the present automation systems for finding necessary information in the patent databases and identifies their advantages and disadvantages. It gives a description of the “Intellect” system, which is an example of software systems for the conceptual design stage support. Materials and Methods The article presents some of the possible ways to organize the patents-analogues search process and specific features of searching analogues and prototypes for the generated parametric structure scheme of the technical solution, which is the result of the synthesis of a new information-measuring and control system element in the “Intellect” system. The description of the proposed search query forming method is given. The article gives the structure of the patent passport, which must be stored in a database to organize the process of searcing analogues and prototypes. There given a description of algorithms for automatic adding a patent to the database, recalculating the weights while adding a patent by experts, identifying the fact of using different physical and technical effects in a patent. Results The final part of the article contains an example of the results of testing the developed subsystem implementing the proposed method. According to the test results it is concluded that the selected software and algorithmic solutions are effective.

  8. Technical problems to be solved before the solid oxide fuel cell will be commercialized

    Energy Technology Data Exchange (ETDEWEB)

    Bagger, C.; Hendriksen, P.V.; Mogensen, M. [Riso National Lab., Roskilde (Denmark)

    1996-12-31

    The problems which must be solved before SOFC-systems are competitive with todays power production technology are of both technical and economical nature. The cost of SOFC stacks at the 25 kW level of today is about 30,000 ECU/kW and it is bound to come down to about 500 ECU/kW. The allowable cost of a SOFC system is anticipated to be around 1500 ECU/kW. As part of the Danish SOFC program (DK-SOFC) a 0.5 kW stack was built and tested during the second half of 1995. Based upon the experience gained, an economic analysis has been made. The tools required to approach an economically acceptable solution are outlined below.

  9. Microfluidics and cancer analysis: cell separation, cell/tissue culture, cell mechanics, and integrated analysis systems.

    Science.gov (United States)

    Pappas, Dimitri

    2016-01-21

    Among the growing number of tools available for cancer studies, microfluidic systems have emerged as a promising analytical tool to elucidate cancer cell and tumor function. Microfluidic methods to culture cells have created approaches to provide a range of environments from single-cell analysis to complex three-dimensional devices. In this review we discuss recent advances in tumor cell culture, cancer cell analysis, and advanced studies enabled by microfluidic systems.

  10. Modern Methods of Multidimensional Data Visualization: Analysis, Classification, Implementation, and Applications in Technical Systems

    Directory of Open Access Journals (Sweden)

    I. K. Romanova

    2016-01-01

    Full Text Available The article deals with theoretical and practical aspects of solving the problem of visualization of multidimensional data as an effective means of multivariate analysis of systems. Several classifications are proposed for visualization techniques, according to data types, visualization objects, the method of transformation of coordinates and data. To represent classification are used charts with links to the relevant work. The article also proposes two classifications of modern trends in display technology, including integration of visualization techniques as one of the modern trends of development, along with the introduction of interactive technologies and the dynamics of development processes. It describes some approaches to the visualization problem, which are concerned with fulfilling the needs. The needs are generated by the relevant tasks such as information retrieval in global networks, development of bioinformatics, study and control of business processes, development of regions, etc. The article highlights modern visualization tools, which are capable of improving the efficiency of the multivariate analysis and searching for solutions in multi-objective optimization of technical systems, but are not very actively used for such studies. These are horizontal graphs, graphics "quantile-quantile", etc. The paper proposes to use Choropleth cards traditionally used in cartography for simultaneous presentation of the distribution parameters of several criteria in the space. It notes that visualizations of graphs in network applications can be more actively used to describe the control system. The article suggests using the heat maps to provide graphical representation of the sensitivity of the system quality criteria under variations of options (multivariate analysis of technical systems. It also mentions that it is useful to extend the supervising heat maps to the task of estimating quality of identify in constructing system models. A

  11. Space solar cells - tradeoff analysis

    Energy Technology Data Exchange (ETDEWEB)

    Reddy, M.R. [ISRO Satellite Centre, Bangalore (India). Power Systems Group

    2003-05-15

    This paper summarizes the study that had the objective to tradeoff space solar cells and solar array designs to determine the best choice of solar cell and array technology that would be more beneficial in terms of mass, area and cost for different types of space missions. Space solar cells, which are commercially now available in the market and to be available in the near future, were considered for this trade study. Four solar array designs: rigid, flexible, thin film flexible and concentrator solar arrays were considered for assessment. Performance of the solar cells along with solar array designs were studied for two types of space missions:geo synchronous orbit (GEO) and low earth orbit (LEO) spacecraft. The Solar array designs assumed were to provide 15 kW power for 15 years mission life in GEO and 5 kW power for 5 years mission life in LEO altitudes. To perform tradeoff analysis a spread sheet model was developed that calculates the size, mass and estimates the cost of solar arrays based on different solar cell and array technologies for given set of mission requirements. Comparative performance metrics (W/kg, W/m{sup 2}, kg/m{sup 2}, and $/W) were calculated for all solar arrays studied and compared, at the solar array subsystem level and also at the spacecraft system level. The trade analysis results show that high-efficiency multijunction solar cells bring lot of cost advantages for both types of missions. The trade study also shows that thin film solar cells with moderate efficiency with ultra lightweight flexible array design may become competitive with well-established single crystalline solar cell technologies in the future. (author)

  12. Space solar cells. Tradeoff analysis

    Energy Technology Data Exchange (ETDEWEB)

    Reddy, M. Raja [Power Systems Group, Solar Panels Division, ISRO Satellite Centre, Bangalore 560017 (India)

    2003-05-15

    This paper summarizes the study that had the objective to tradeoff space solar cells and solar array designs to determine the best choice of solar cell and array technology that would be more beneficial in terms of mass, area and cost for different types of space missions. Space solar cells, which are commercially now available in the market and to be available in the near future, were considered for this trade study. Four solar array designs: rigid, flexible, thin film flexible and concentrator solar arrays were considered for assessment. Performance of the solar cells along with solar array designs were studied for two types of space missions: geo synchronous orbit (GEO) and low earth orbit (LEO) spacecraft. The Solar array designs assumed were to provide 15kW power for 15 years mission life in GEO and 5kW power for 5 years mission life in LEO altitudes. To perform tradeoff analysis a spread sheet model was developed that calculates the size, mass and estimates the cost of solar arrays based on different solar cell and array technologies for given set of mission requirements. Comparative performance metrics (W/kg, W/m{sup 2}, kg/m{sup 2}, and $/W) were calculated for all solar arrays studied and compared, at the solar array subsystem level and also at the spacecraft system level. The trade analysis results show that high-efficiency multijunction solar cells bring lot of cost advantages for both types of missions. The trade study also show that thin film solar cells with moderate efficiency with ultra lightweight flexible array design may become competitive with well-established single crystalline solar cell technologies in the future.

  13. Flight Technical Error Analysis of the SATS Higher Volume Operations Simulation and Flight Experiments

    Science.gov (United States)

    Williams, Daniel M.; Consiglio, Maria C.; Murdoch, Jennifer L.; Adams, Catherine H.

    2005-01-01

    This paper provides an analysis of Flight Technical Error (FTE) from recent SATS experiments, called the Higher Volume Operations (HVO) Simulation and Flight experiments, which NASA conducted to determine pilot acceptability of the HVO concept for normal operating conditions. Reported are FTE results from simulation and flight experiment data indicating the SATS HVO concept is viable and acceptable to low-time instrument rated pilots when compared with today s system (baseline). Described is the comparative FTE analysis of lateral, vertical, and airspeed deviations from the baseline and SATS HVO experimental flight procedures. Based on FTE analysis, all evaluation subjects, low-time instrument-rated pilots, flew the HVO procedures safely and proficiently in comparison to today s system. In all cases, the results of the flight experiment validated the results of the simulation experiment and confirm the utility of the simulation platform for comparative Human in the Loop (HITL) studies of SATS HVO and Baseline operations.

  14. Study on the Technical Efficiency of Creative Human Capital in China by Three-Stage Data Envelopment Analysis Model

    Directory of Open Access Journals (Sweden)

    Jian Ma

    2014-01-01

    Full Text Available Previous researches have proved the positive effect of creative human capital and its development on the development of economy. Yet, the technical efficiency of creative human capital and its effects are still under research. The authors are trying to estimate the technical efficiency value in Chinese context, which is adjusted by the environmental variables and statistical noises, by establishing a three-stage data envelopment analysis model, using data from 2003 to 2010. The research results indicate that, in this period, the entirety of creative human capital in China and the technical efficiency value in different regions and different provinces is still in the low level and could be promoted. Otherwise, technical non-efficiency is mostly derived from the scale nonefficiency and rarely affected by pure technical efficiency. The research also examines environmental variables’ marked effects on the technical efficiency, and it shows that different environmental variables differ in the aspect of their own effects. The expansion of the scale of education, development of healthy environment, growth of GDP, development of skill training, and population migration could reduce the input of creative human capital and promote the technical efficiency, while development of trade and institutional change, on the contrary, would block the input of creative human capital and the promotion the technical efficiency.

  15. Technical Insights into Highly Sensitive Isolation and Molecular Characterization of Fixed and Live Circulating Tumor Cells for Early Detection of Tumor Invasion

    Science.gov (United States)

    Laget, Sophie; Dhingra, Dalia M.; BenMohamed, Fatima; Capiod, Thierry; Osteras, Magne; Farinelli, Laurent; Jackson, Stephen; Paterlini-Bréchot, Patrizia

    2017-01-01

    Circulating Tumor Cells (CTC) and Circulating Tumor Microemboli (CTM) are Circulating Rare Cells (CRC) which herald tumor invasion and are expected to provide an opportunity to improve the management of cancer patients. An unsolved technical issue in the CTC field is how to obtain highly sensitive and unbiased collection of these fragile and heterogeneous cells, in both live and fixed form, for their molecular study when they are extremely rare, particularly at the beginning of the invasion process. We report on a new protocol to enrich from blood live CTC using ISET® (Isolation by SizE of Tumor/Trophoblastic Cells), an open system originally developed for marker-independent isolation of fixed tumor cells. We have assessed the impact of our new enrichment method on live tumor cells antigen expression, cytoskeleton structure, cell viability and ability to expand in culture. We have also explored the ISET® in vitro performance to collect intact fixed and live cancer cells by using spiking analyses with extremely low number of fluorescent cultured cells. We describe results consistently showing the feasibility of isolating fixed and live tumor cells with a Lower Limit of Detection (LLOD) of one cancer cell per 10 mL of blood and a sensitivity at LLOD ranging from 83 to 100%. This very high sensitivity threshold can be maintained when plasma is collected before tumor cells isolation. Finally, we have performed a comparative next generation sequencing (NGS) analysis of tumor cells before and after isolation from blood and culture. We established the feasibility of NGS analysis of single live and fixed tumor cells enriched from blood by our system. This study provides new protocols for detection and characterization of CTC collected from blood at the very early steps of tumor invasion. PMID:28060956

  16. Microfluidic Cell Cycle Analysis of Spread Cells by DAPI Staining

    OpenAIRE

    Jing Sun; Jiayu Zhang; Haibo Yang; Gongzhuo Wang; Yanzhao Li; Xuxin Zhang; Qidan Chen; Ming-Fei Lang

    2017-01-01

    Single-cell cell cycle analysis is an emerging technique that requires detailed exploration of the image analysis process. In this study, we established a microfluidic single-cell cell cycle analysis method that can analyze cells in small numbers and in situ on a microfluidic chip. In addition, factors that influenced the analysis were carefully investigated. U87 or HeLa cells were seeded and attached to microfluidic channels before measurement. Cell nucleic DNA was imaged by 4′-6-diamidino-2...

  17. Comparisons: Technical-Tactical and Time-Motion Analysis of Mixed Martial Arts by Outcomes.

    Science.gov (United States)

    Miarka, Bianca; Vecchio, Fabrício B D; Camey, Suzi; Amtmann, John A

    2016-07-01

    Miarka, B, Vecchio, FBD, Camey, S, and Amtmann, JA. Comparisons: technical-tactical and time-motion analysis of mixed martial arts by outcomes. J Strength Cond Res 30(7): 1975-1984, 2016-The aim of this study was to compare time-motion and technical-tactical analysis between paired outcomes and rounds of mixed martial arts (MMA) matches. The sample consisted of 645 rounds of MMA competition paired by outcomes (first round, winners n = 215 and losers n = 215; second round, winners n = 215 and losers n = 215; third round, winners n = 215 and losers n = 215). The time-motion variables were categorized into low-intensity or high-intensity, stand-up or groundwork situations. Stand-up techniques were analyzed by observing total strikes to the head and body, and takedowns. The actions on the ground were analyzed by observing submission activity, including successful choking and joint locking actions, and also positional improvements, including advances to the mount, half guard, and side and back positions. Chi-squared and Wilcoxon tests were conducted with a significance level of p ≤ 0.05. Results showed that winners had higher values for total strikes and submissions in all rounds, and also positional improvements, over losers. The standing combat with low-intensity comparisons presented differences between the rounds first, with a median of 2:33.5 (P25-P75%: 1:20-3:56) minute, second, with 2:37 (1:24-3:59) minute, and third, with 2:07 (1:06-3:39.2) minute. These data suggest a focus on the intermittent demand presented in combat phases with a special attention to the strike and ground technical-tactical skills; strength and conditioning coaches could emphasize the effort pause ratios for both standing and ground combat that mimic the requirements of MMA, especially during the third round.

  18. Overview and analysis of content of technical drawing in textbooks and working books in courses of Design and Technology

    OpenAIRE

    2012-01-01

    Technical drawing is a subject in primary schools from sixth to eight grades. In my diploma I presented some key words and rules of technical drawing. I also overviewed the content used in current study plan. Overview of textbooks and working books is based on how they are used in study plans and how they apply the rules of technical drawing. Analysis of the results showed that only few textbooks and working books are covering all study plans and that few drawings in textbooks ...

  19. Identification and Stochastic Analysis of Factors Influencing Technical Inefficiency of Nigerian Smallholder Soybean Farmers

    Directory of Open Access Journals (Sweden)

    Otitoju, MA.

    2014-01-01

    Full Text Available Smallholder soybean production is investigated using an econometric analysis otherwise known as stochastic frontier analysis through transcendental logarithmic (translog production function, which incorporates an inefficiency effects model. Ninety-six farmers were randomly selected through multistage techniques in Benue State, Nigeria. Factors (socio-economic and institutional considered in the inefficiency effects model include household size, sex, age, years of schooling, farming experience in soybean production, health status, off-farm employment, non-family labour, credit accessibility, land fragmentation and extension contact. The parameters of the stochastic frontier translog production function are estimated contemporaneously with those involved in the inefficiency effects model. The results indicate that household size, age, non-family labour were significant and negatively related to the technical inefficiency while farming experience, off-farm employment, credit accessibility, land fragmentation, and extension contact were statistically significant and positively related to the inefficiency. The mean technical efficiency of the farmers is 0.84. This means that the farmers can still improve their efficiency level by 16%.

  20. Study on the Technical Efficiency of Creative Human Capital in China by Three-Stage Data Envelopment Analysis Model

    OpenAIRE

    Jian Ma; Yueru Ma; Yong Bai; Bing Xia

    2014-01-01

    Previous researches have proved the positive effect of creative human capital and its development on the development of economy. Yet, the technical efficiency of creative human capital and its effects are still under research. The authors are trying to estimate the technical efficiency value in Chinese context, which is adjusted by the environmental variables and statistical noises, by establishing a three-stage data envelopment analysis model, using data from 2003 to 2010. The research resul...

  1. On sustainability assessment of technical systems. Experience from systems analysis with the ORWARE and Ecoeffect tools

    Energy Technology Data Exchange (ETDEWEB)

    Assefa, Getachew [Royal Inst. of Technology, Stockholm (Sweden). Dept. of Chemical Engineering

    2006-06-15

    Engineering research and development work is undergoing a reorientation from focusing on specific parts of different systems to a broader perspective of systems level, albeit at a slower pace. This reorientation should be further developed and enhanced with the aim of organizing and structuring our technical systems in meeting sustainability requirements in face of global ecological threats that have far-reaching social and economic implications, which can no longer be captured using conventional approach of research. Until a list of universally acceptable, clear, and measurable indicators of sustainable development is developed, the work with sustainability metrics should continue to evolve as a relative measure of ecological, economic, and social performance of human activities in general, and technical systems in particular. This work can be done by comparing the relative performance of alternative technologies of providing the same well-defined function or service; or by characterizing technologies that enjoy different levels of societal priorities using relevant performance indicators. In both cases, concepts and methods of industrial ecology play a vital role. This thesis is about the development and application of a systematic approach for the assessment of the performance of technical systems from the perspective of systems analysis, sustainability, sustainability assessment, and industrial ecology. The systematic approach developed and characterized in this thesis advocates for a simultaneous assessment of the ecological, economic, and social dimensions of performance of technologies in avoiding sub-optimization and problem shifting between dimensions. It gives a holistic picture by taking a life cycle perspective of all important aspects. The systematic assessment of technical systems provides an even-handed assessment resulting in a cumulative knowledge. A modular structure of the approach makes it flexible enough in terms of comparing a number of

  2. Technical Requirements Analysis and Control Systems (TRACS) Initial Operating Capability (IOC) documentation

    Science.gov (United States)

    Hammond, Dana P.

    1991-01-01

    The Technical Requirements Analysis and Control Systems (TRACS) software package is described. TRACS offers supplemental tools for the analysis, control, and interchange of project requirements. This package provides the fundamental capability to analyze and control requirements, serves a focal point for project requirements, and integrates a system that supports efficient and consistent operations. TRACS uses relational data base technology (ORACLE) in a stand alone or in a distributed environment that can be used to coordinate the activities required to support a project through its entire life cycle. TRACS uses a set of keyword and mouse driven screens (HyperCard) which imposes adherence through a controlled user interface. The user interface provides an interactive capability to interrogate the data base and to display or print project requirement information. TRACS has a limited report capability, but can be extended with PostScript conventions.

  3. Technical analysis of boarding equipment%港口登船设备技术分析

    Institute of Scientific and Technical Information of China (English)

    胡思唐

    2012-01-01

    随着邮轮旅游业的快速发展,传统客运港或现代邮轮港对登船设备的技术水平和性能需求都提出了更高的要求.本文在阐述港口登船设备需求趋势的基础上,介绍和分析了国内外多种典型港口登船设备的结构特点、适用范围及其对需求趋势的适应性.通过技术分析比较,总结了现代化邮轮旅客登船桥的最新技术特点和应用趋势,可为邮轮港口码头的规划建设和工艺设备选型提供参考.%With the rapid development of cruise tourism, the higher technical level and performance of boarding equipment are required for traditional passenger port or modern cruise ship port. The paper introduces and analyzes structural properties, scope of application and adaptability to demand trend for various typical types of port boarding equipment at home and abroad based on the description of demand trend of such boarding equipment. Through technical analysis and comparison, the latest technical properties and application trend are summarized for modern cruise passenger boarding bridges, to provide references for planning, construction, and selection of processing equipment of cruise ports.

  4. Lithium-thionyl chloride cell system safety hazard analysis

    Science.gov (United States)

    Dampier, F. W.

    1985-03-01

    This system safety analysis for the lithium thionyl chloride cell is a critical review of the technical literature pertaining to cell safety and draws conclusions and makes recommendations based on this data. The thermodynamics and kinetics of the electrochemical reactions occurring during discharge are discussed with particular attention given to unstable SOCl2 reduction intermediates. Potentially hazardous reactions between the various cell components and discharge products or impurities that could occur during electrical or thermal abuse are described and the most hazardous conditions and reactions identified. Design factors influencing the safety of Li/SOCl2 cells, shipping and disposal methods and the toxicity of Li/SOCl2 battery components are additional safety issues that are also addressed.

  5. A review and evaluation of the Langley Research Center's Scientific and Technical Information Program: Results of phase 6: The technical report. A survey and analysis

    Science.gov (United States)

    Mccullough, R. A.; Pinelli, T. E.; Pilley, D. D.; Stohrer, F. F.

    1982-01-01

    Current practice and usage using selected technical reports; literature relative to the sequential, language, and presentation components of technical reports; and NASA technical report publications standards are discussed. The effctiveness of the technical report as a product for information dissemination is considered.

  6. Technical Efficiency Analysis of Container Terminals in the Middle Eastern Region

    Directory of Open Access Journals (Sweden)

    Ebrahim Sharaf Almawsheki

    2015-12-01

    Full Text Available Despite an increasing number of studies on the efficiency of container terminals, their focus has mostly been on advanced and emerging markets. There are limited studies on container terminals in developing countries such as those of the Middle Eastern region, which are located in a critical geographic position in the international maritime route between the East and the West. Information on their potential for development relative to other terminals worldwide is thus not readily available. This study aims to evaluate the technical efficiency of 19 container terminals in the Middle Eastern region. The DEA approach is used to measure technical efficiency, and slack variable analysis identifies potential areas of improvement for inefficient terminals. The results show that the Jebel Ali, Salalah and Beirut container terminals are the most efficient terminals in the region, and that the least efficient is the terminal in Aden. The results provide valuable information for terminal managers, helping to develop resource utilisation for steady development in operational efficiency.

  7. Technical program to study the benefits of nonlinear analysis methods in LWR component designs. Technical report TR-3723-1

    Energy Technology Data Exchange (ETDEWEB)

    Raju, P. P.

    1980-05-01

    This report summarizes the results of the study program to assess the benefits of nonlinear analysis methods in Light Water Reactor (LWR) component designs. The current study reveals that despite its increased cost and other complexities, nonlinear analysis is a practical and valuable tool for the design of LWR components, especially under ASME Level D service conditions (faulted conditions) and it will greatly assist in the evaluation of ductile fracture potential of pressure boundary components. Since the nonlinear behavior is generally a local phenomenon, the design of complex components can be accomplished through substructuring isolated localized regions and evaluating them in detail using nonlinear analysis methods.

  8. Microfluidic Cell Cycle Analysis of Spread Cells by DAPI Staining

    Directory of Open Access Journals (Sweden)

    Jing Sun

    2017-01-01

    Full Text Available Single-cell cell cycle analysis is an emerging technique that requires detailed exploration of the image analysis process. In this study, we established a microfluidic single-cell cell cycle analysis method that can analyze cells in small numbers and in situ on a microfluidic chip. In addition, factors that influenced the analysis were carefully investigated. U87 or HeLa cells were seeded and attached to microfluidic channels before measurement. Cell nucleic DNA was imaged by 4′-6-diamidino-2-phenylindole (DAPI staining under a fluorescent microscope and subsequently fluorescent intensities of the cell nuclei DNA were converted to depict histograms for cell cycle phases. DAPI concentration, microscopic magnification, exposure time and cell number were examined for optimal cell cycle analysis conditions. The results showed that as few as a few hundred cells could be measured by DAPI staining in the range of 0.4–0.6 μg/mL to depict histograms with typical cell cycle phase distribution. Microscopic magnification during image acquisition, however, could distort the phase distribution. Exposure time did not significantly affect the cell cycle analysis. Furthermore, cell cycle inhibitor rapamycin treatment changed the cell cycle phase distribution as expected. In conclusion, a method for microfluidic single-cell cell cycle analysis of spread cells in situ was developed. Factors such as dye concentration and microscopic magnification had more influence on cell cycle phase distribution. Further studies will focus on detail differentiation of cell cycle phases and the application of such a method for biological meanings.

  9. Social pragmatics in technical writing: a corpus-based analysis of thematic articles

    Directory of Open Access Journals (Sweden)

    Carmen Pérez-Llantada Auría

    2003-04-01

    Full Text Available The main objective of this paper is to analyse some sociocultural implications involved in the process of technical writing. In particular, the analysis will focus on those socially and ideologically-related rhetorical mechanisms of linguistic interaction that engineers use when writing thematic articles. As a selected corpus maps out, concepts such as ideology, power, politeness or a persuasive rhetoric prove to be key factors in determining the appropriate linguistic choices in those social interactions within this community. In the light of social pragmatics, the extent to which institutional and cultural factors affect research writing in the field of technology will therefore call for a redefinition of the ‘classical’ objectivity sought in these specialised discourse practices.

  10. Fractional Black-Scholes Model and Technical Analysis of Stock Price

    Directory of Open Access Journals (Sweden)

    Song Xu

    2013-01-01

    Full Text Available In the stock market, some popular technical analysis indicators (e.g., Bollinger bands, RSI, ROC, etc. are widely used to forecast the direction of prices. The validity is shown by observed relative frequency of certain statistics, using the daily (hourly, weekly, etc. stock prices as samples. However, those samples are not independent. In earlier research, the stationary property and the law of large numbers related to those observations under Black-Scholes stock price model and stochastic volatility model have been discussed. Since the fitness of both Black-Scholes model and short-range dependent process has been questioned, we extend the above results to fractional Black-Scholes model with Hurst parameter H>1/2, under which the stock returns follow a kind of long-range dependent process. We also obtain the rate of convergence.

  11. Dosimetric and technical aspects of intraoperative I-125 brachytherapy for stage I non-small cell lung cancer

    Energy Technology Data Exchange (ETDEWEB)

    Johnson, Mark [Department of Radiation Oncology, Allegheny General Hospital, Pittsburgh, Pennsylvania 15212 (United States); Colonias, Athanasios [Department of Radiation Oncology, Allegheny General Hospital, Pittsburgh, Pennsylvania 15212 (United States); Parda, David [Department of Radiation Oncology, Allegheny General Hospital, Pittsburgh, Pennsylvania 15212 (United States); Trombetta, Mark [Department of Radiation Oncology, Allegheny General Hospital, Pittsburgh, Pennsylvania 15212 (United States); Gayou, Olivier [Department of Radiation Oncology, Allegheny General Hospital, Pittsburgh, Pennsylvania 15212 (United States); Reitz, Bodo [Department of Radiation Oncology, Allegheny General Hospital, Pittsburgh, Pennsylvania 15212 (United States); Miften, Moyed [Department of Radiation Oncology, Allegheny General Hospital, Pittsburgh, Pennsylvania 15212 (United States)

    2007-03-07

    Initial treatment outcome data from our institution for stage I non-small cell lung cancer (NSCLC) patients have shown that sublobar resection in combination with iodine-125 (I-125) brachytherapy is associated with recurrence rates of 2.0%, compared to 18.6% with sublobar resection alone. In this work, the technical and dosimetric aspects required to execute this procedure from the radiation oncology perspective as well as an analysis of the dose distributions of patients treated with this technique are presented. In this treatment technique, I-125 seeds in vicryl suture are embedded into vicryl mesh and surgically inserted providing a 2.0 cm margin on each side of the resection staple line. A nomogram is developed to determine the suture spacing in the vicryl mesh, as a function of seed activity in order to deliver 120 Gy at a distance of 0.5 cm above and below the seed array. Post-operative dosimetry consists of a CT-based planning and dose volume analysis. Dose distributions, dose volume histograms and mean dose data for lung are analysed in a group of patients. Dosimetric results show significant lung sparing with only a small volume of lung irradiated for all patients with mean lung dose values ranging from 1.5 Gy to 5.4 Gy. Lung brachytherapy with I-125 at the time of sublobar resection is a highly conformal option of dose delivery for stage I NSCLC patients with compromised physiologic reserve. Patient-related toxicity clinically measured by loss of pulmonary function and radiation-induced pneumonitis have not been linked to this procedure.

  12. Dosimetric and technical aspects of intraoperative I-125 brachytherapy for stage I non-small cell lung cancer

    Science.gov (United States)

    Johnson, Mark; Colonias, Athanasios; Parda, David; Trombetta, Mark; Gayou, Olivier; Reitz, Bodo; Miften, Moyed

    2007-03-01

    Initial treatment outcome data from our institution for stage I non-small cell lung cancer (NSCLC) patients have shown that sublobar resection in combination with iodine-125 (I-125) brachytherapy is associated with recurrence rates of 2.0%, compared to 18.6% with sublobar resection alone. In this work, the technical and dosimetric aspects required to execute this procedure from the radiation oncology perspective as well as an analysis of the dose distributions of patients treated with this technique are presented. In this treatment technique, I-125 seeds in vicryl suture are embedded into vicryl mesh and surgically inserted providing a 2.0 cm margin on each side of the resection staple line. A nomogram is developed to determine the suture spacing in the vicryl mesh, as a function of seed activity in order to deliver 120 Gy at a distance of 0.5 cm above and below the seed array. Post-operative dosimetry consists of a CT-based planning and dose volume analysis. Dose distributions, dose volume histograms and mean dose data for lung are analysed in a group of patients. Dosimetric results show significant lung sparing with only a small volume of lung irradiated for all patients with mean lung dose values ranging from 1.5 Gy to 5.4 Gy. Lung brachytherapy with I-125 at the time of sublobar resection is a highly conformal option of dose delivery for stage I NSCLC patients with compromised physiologic reserve. Patient-related toxicity clinically measured by loss of pulmonary function and radiation-induced pneumonitis have not been linked to this procedure.

  13. Imagining Technicities

    DEFF Research Database (Denmark)

    Liboriussen, Bjarke; Plesner, Ursula

    2011-01-01

    , this article focuses on innovative uses of virtual worlds in architecture. We interviewed architects, industrial designers and other practitioners. Conceptually supported by an understanding of technicity found in Cultural Studies, the interviews were then coded with a focus on interviewees’ references......The actors of the building industry have access to a range of Information and Communication Technologies (ICTs), and are constantly presented with new software and new communications platforms. Through case studies, and inspired by sociotechnical approaches to the study of emerging technologies...... to the elements of taste and skill. In the final analysis those references were synthesized as five imagined technicities: the architect, the engineer, the client, the Chinese, and the Virtual World native. Because technicities are often assumed and rarely discussed as actants who influence practice, their role...

  14. High-temperature fuel cell research and development. Final technical status report, June 1977-September 1978

    Energy Technology Data Exchange (ETDEWEB)

    1978-10-15

    An initial survey of the literature produced a list of ceramic materials with properties which made them potential candidates for use in molten-carbonate fuel cell tiles or electrodes. Seven of the materials in the original list were dropped from consideration because of unfavorable thermodynamic properties; four materials were set aside because of high cost, lack of availability, or fabrication difficulties. Thirteen compositions were tested statically at 1000 K in a Li/sub 2/CO/sub 3/-K/sub 2/CO/sub 3/ bath under a dry CO/sub 2/ atmosphere. Only four of the materials tested showed severe degradation reactions in the molten carbonate. A low-temperature process for forming small diameter, high-aspect ratio ceramic fibers for fuel cell use has been developed. A short-term program to initiate a computer study on the thermodynamic analysis of fuel cell materials was initiated at Montana State University. The report on this program is included as Appendix B. The MHD and high-temperature fuel cell literature was surveyed, and material properties were evaluated to identify MHD materials with potential use for fuel cell applications. A technology transfer report of these findings was prepared. This report is included as Appendix A. Laboratory facilities were established to conduct research on interfacial diffusion processes which could be detrimental to successful long-term operation of the solid-electrolyte fuel cell. A variety of physical and chemical techniques were examined for the preparation of high-density substituted LaCrO/sub 3/ which was to be one component of a diffusion couple with Y/sub 2/O/sub 3/-stabilized ZrO/sub 2/. Hydrolysis of a mixed metal-nitrate solution with urea produced the most reactive powder. A final theoretical density of almost 98% was attained in cold-pressed sintered discs of this material. (Extensive list of references)

  15. Use of technical and economic analysis in production of liquid metal in foundries

    Directory of Open Access Journals (Sweden)

    V. Kafka

    2008-07-01

    Full Text Available The paper in its introduction describes main principles of technical and economic analysis the application of which leads to determination of potential costs savings and subsequently to costs reduction. The application of the method is illustrated by an example of production of three types of steel grades for cast steel produced in five tons electric arc furnace. With the aid of the calculation model, incomplete costs of selective complex of the melts were determined (30 melts within the first phase and 260 in the second one. Incomplete costs and selected physical indicators (e.g. melting time, electrical energy consumption etc. were subsequently compiled with the aid of statistical analysis. After that, the individual selective complexes were compared with each other (according to melters; first and second melt on the shift; melting with one or more charge basket; days in a week. The detailed analysis of these outcomes resulted in many particular recommendations how to reduce the costs in the foundry. The final recommendation for the melting shop is a proposal to introduce continuous monitoring of costs.

  16. Program of scientific investigations and development of solid-oxide fuel cells (SOFC) in VNIITF. Proposals on scientific and technical collaboration and SOFC commercialization

    Energy Technology Data Exchange (ETDEWEB)

    Kleschev, Yu.N.; Chukharev, V.F.

    1996-04-01

    This paper describes proposals on scientific and technical collaborations pertaining to solid oxide fuel cell commercialization. Topics included for discussion are: materials research and manufacture; market estimation and cost; directions of collaboration; and project of proposals on joint enterprise creation.

  17. Technical Advance: Transcription factor, promoter, and enhancer utilization in human myeloid cells.

    Science.gov (United States)

    Joshi, Anagha; Pooley, Christopher; Freeman, Tom C; Lennartsson, Andreas; Babina, Magda; Schmidl, Christian; Geijtenbeek, Teunis; Michoel, Tom; Severin, Jessica; Itoh, Masayoshi; Lassmann, Timo; Kawaji, Hideya; Hayashizaki, Yoshihide; Carninci, Piero; Forrest, Alistair R R; Rehli, Michael; Hume, David A

    2015-05-01

    The generation of myeloid cells from their progenitors is regulated at the level of transcription by combinatorial control of key transcription factors influencing cell-fate choice. To unravel the global dynamics of this process at the transcript level, we generated transcription profiles for 91 human cell types of myeloid origin by use of CAGE profiling. The CAGE sequencing of these samples has allowed us to investigate diverse aspects of transcription control during myelopoiesis, such as identification of novel transcription factors, miRNAs, and noncoding RNAs specific to the myeloid lineage. We further reconstructed a transcription regulatory network by clustering coexpressed transcripts and associating them with enriched cis-regulatory motifs. With the use of the bidirectional expression as a proxy for enhancers, we predicted over 2000 novel enhancers, including an enhancer 38 kb downstream of IRF8 and an intronic enhancer in the KIT gene locus. Finally, we highlighted relevance of these data to dissect transcription dynamics during progressive maturation of granulocyte precursors. A multifaceted analysis of the myeloid transcriptome is made available (www.myeloidome.roslin.ed.ac.uk). This high-quality dataset provides a powerful resource to study transcriptional regulation during myelopoiesis and to infer the likely functions of unannotated genes in human innate immunity.

  18. Technical Advance: Transcription factor, promoter, and enhancer utilization in human myeloid cells

    Science.gov (United States)

    Joshi, Anagha; Pooley, Christopher; Freeman, Tom C.; Lennartsson, Andreas; Babina, Magda; Schmidl, Christian; Geijtenbeek, Teunis; Michoel, Tom; Severin, Jessica; Itoh, Masayoshi; Lassmann, Timo; Kawaji, Hideya; Hayashizaki, Yoshihide; Carninci, Piero; Forrest, Alistair R. R.; Rehli, Michael; Hume, David A.

    2015-01-01

    The generation of myeloid cells from their progenitors is regulated at the level of transcription by combinatorial control of key transcription factors influencing cell-fate choice. To unravel the global dynamics of this process at the transcript level, we generated transcription profiles for 91 human cell types of myeloid origin by use of CAGE profiling. The CAGE sequencing of these samples has allowed us to investigate diverse aspects of transcription control during myelopoiesis, such as identification of novel transcription factors, miRNAs, and noncoding RNAs specific to the myeloid lineage. We further reconstructed a transcription regulatory network by clustering coexpressed transcripts and associating them with enriched cis-regulatory motifs. With the use of the bidirectional expression as a proxy for enhancers, we predicted over 2000 novel enhancers, including an enhancer 38 kb downstream of IRF8 and an intronic enhancer in the KIT gene locus. Finally, we highlighted relevance of these data to dissect transcription dynamics during progressive maturation of granulocyte precursors. A multifaceted analysis of the myeloid transcriptome is made available (www.myeloidome.roslin.ed.ac.uk). This high-quality dataset provides a powerful resource to study transcriptional regulation during myelopoiesis and to infer the likely functions of unannotated genes in human innate immunity. PMID:25717144

  19. Data development technical support document for the aircraft crash risk analysis methodology (ACRAM) standard

    Energy Technology Data Exchange (ETDEWEB)

    Kimura, C.Y.; Glaser, R.E.; Mensing, R.W.; Lin, T.; Haley, T.A.; Barto, A.B.; Stutzke, M.A.

    1996-08-01

    The Aircraft Crash Risk Analysis Methodology (ACRAM) Panel has been formed by the US Department of Energy Office of Defense Programs (DOE/DP) for the purpose of developing a standard methodology for determining the risk from aircraft crashes onto DOE ground facilities. In order to accomplish this goal, the ACRAM panel has been divided into four teams, the data development team, the model evaluation team, the structural analysis team, and the consequence team. Each team, consisting of at least one member of the ACRAM plus additional DOE and DOE contractor personnel, specializes in the development of the methodology assigned to that team. This report documents the work performed by the data development team and provides the technical basis for the data used by the ACRAM Standard for determining the aircraft crash frequency. This report should be used to provide the generic data needed to calculate the aircraft crash frequency into the facility under consideration as part of the process for determining the aircraft crash risk to ground facilities as given by the DOE Standard Aircraft Crash Risk Assessment Methodology (ACRAM). Some broad guidance is presented on how to obtain the needed site-specific and facility specific data but this data is not provided by this document.

  20. Face haulage equipment failure analysis. Volume I. Technical information and conclusions. Final technical report as of November 30, 1980

    Energy Technology Data Exchange (ETDEWEB)

    Patterson, W.N.; Orona, F.

    1980-11-01

    Face haulage equipment used in conjunction with continuous miners (shuttle cars, diesel haulers, battery scoops, and bridge conveyors) was investigated by recording section delay reports for computer analysis to determine the effect of haulage equipment failures and downtime on productivity, pinpoint the causes of machine failures and downtime, and develop the possible design and operational changes required to reduce machine failures and downtime and increase section productivity. For the mobile vehicle type of haulage (shuttle car, diesel hauler, and battery scoop) failure of one unit in multiple unit haulage operations would not normally stop section production. Bridge conveyors as a haulage system provide continuous haulage of section production but when any part of the bridge system fails, the section production is stopped. In the course of this program, it was determined through the use of daily section shift reports on 200 machines that face haulage equipment is responsible for about 40 to 56 minutes of lost section production time per shift. The most prevalent failure for shuttle cars was found to be the trailing cable umbilical. Bridge conveyors had the most trouble with the conveyor subsystem. Discussions of these and other recorded failures are developed with possible solutions outlined for future implementation. This report only covers the face haulage element of the continuous miner system. Companion reports were developed for the continuous miner and roof bolter elements under separate task orders.

  1. Structure in Community College Career-Technical Programs: A Qualitative Analysis. CCRC Working Paper No. 50

    Science.gov (United States)

    Van Noy, Michelle; Weiss, Madeline Joy; Jenkins, Davis; Barnett, Elisabeth A.; Wachen, John

    2012-01-01

    Using data obtained from interviews and program websites at Washington community and technical colleges, the authors of this study examine the structure of community college career-technical programs in allied health, business and marketing, computer and information studies, and mechanics and repair. A framework for structure with four…

  2. Recent Advances in Morphological Cell Image Analysis

    Directory of Open Access Journals (Sweden)

    Shengyong Chen

    2012-01-01

    Full Text Available This paper summarizes the recent advances in image processing methods for morphological cell analysis. The topic of morphological analysis has received much attention with the increasing demands in both bioinformatics and biomedical applications. Among many factors that affect the diagnosis of a disease, morphological cell analysis and statistics have made great contributions to results and effects for a doctor. Morphological cell analysis finds the cellar shape, cellar regularity, classification, statistics, diagnosis, and so forth. In the last 20 years, about 1000 publications have reported the use of morphological cell analysis in biomedical research. Relevant solutions encompass a rather wide application area, such as cell clumps segmentation, morphological characteristics extraction, 3D reconstruction, abnormal cells identification, and statistical analysis. These reports are summarized in this paper to enable easy referral to suitable methods for practical solutions. Representative contributions and future research trends are also addressed.

  3. Proteomic Analysis of Chinese Hamster Ovary Cells

    DEFF Research Database (Denmark)

    Baycin-Hizal, Deniz; Tabb, David L.; Chaerkady, Raghothama;

    2012-01-01

    To complement the recent genomic sequencing of Chinese hamster ovary (CHO) cells, proteomic analysis was performed on CHO cells including the cellular proteome, secretome, and glycoproteome using tandem mass spectrometry (MS/MS) of multiple fractions obtained from gel electrophoresis, multidimens......To complement the recent genomic sequencing of Chinese hamster ovary (CHO) cells, proteomic analysis was performed on CHO cells including the cellular proteome, secretome, and glycoproteome using tandem mass spectrometry (MS/MS) of multiple fractions obtained from gel electrophoresis...

  4. An independent assessment of the technical feasibility of the Mars One mission plan - Updated analysis

    Science.gov (United States)

    Do, Sydney; Owens, Andrew; Ho, Koki; Schreiner, Samuel; de Weck, Olivier

    2016-03-01

    In recent years, the Mars One program has gained significant publicity for its plans to colonize the red planet. Beginning in 2025, the program plans to land four people on Mars every 26 months via a series of one-way missions, using exclusively existing technology. This one-way approach has frequently been cited as a key enabler of accelerating the first crewed landing on Mars. While the Mars One program has received considerable attention, little has been published in the technical literature regarding the formulation of its mission architecture. In light of this, we perform an independent analysis of the technical feasibility of the Mars One mission plan, focusing on the architecture of the life support and in-situ resource utilization (ISRU) systems, and their impact on sparing and space logistics. To perform this analysis, we adopt an iterative analysis approach in which we model and simulate the mission architecture, assess its feasibility, implement any applicable modifications while attempting to remain within the constraints set forth by Mars One, and then resimulate and reanalyze the revised version of the mission architecture. Where required information regarding the Mars One mission architecture is not available, we assume numerical values derived from standard spaceflight design handbooks and documents. Through four iterations of this process, our analysis finds that the Mars One mission plan, as publicly described, is not feasible. This conclusion is obtained from analyses based on mission assumptions derived from and constrained by statements made by Mars One, and is the result of the following findings: (1) several technologies including ISRU, life support, and entry, descent, and landing (EDL) are not currently "existing, validated and available" as claimed by Mars One; (2) the crop growth area described by Mars One is insufficient to feed their crew; (3) increasing the crop growth area to provide sufficient food for the crew leads to atmospheric

  5. Proceedings of the workshop on structural analysis needs for magnetic fusion energy superconducting magnets: a technical assessment

    Energy Technology Data Exchange (ETDEWEB)

    Reich, M.; Lehner, J.; Powell, J.; Bezler, P.

    1976-01-01

    The technical portions of the meeting were divided into three major sessions as follows: (1) Review of methods being presently used by the MFE community for structural evaluation of current designs. (2) Future structural analysis needs. (3) Open discussions dealing with adequacy of present methods, the improvements needed for MFE magnet structural analysis, and the establishment of an MFE magnet structural advisory group. Summaries of the individual talks are presented.

  6. Structural Analysis of Technical-Tactical Elements in Table Tennis and their Role in Different Playing Zones

    Directory of Open Access Journals (Sweden)

    Munivrana Goran

    2015-09-01

    Full Text Available For the purpose of determining the overall structure of technical-tactical elements in table tennis and evaluating their role in different playing zones around the table, a new measuring instrument (a questionnaire was formulated that took advantage of the expert knowledge of top, world class table tennis coaches. The results of the hierarchical taxonomic (cluster analysis showed that the overall structure of the technical-tactical elements forming the table tennis technique could be divided into three basic groups; a group of technical-tactical elements (A used in the phase of preparing one’s own and disabling the opponent’s attack; a group of technical-tactical elements (B used in the phase of attack and counterattack; and a group of technical-tactical elements (C used in the phase of defense. The differences among the obtained groups of table tennis elements were determined by applying the Kruskal-Wallis test, while relations between the groups and their role in different playing zones around the table were analyzed by comparing the average values of the experts’ scores.

  7. Cell mechanics through analysis of cell trajectories in microfluidic channel

    Science.gov (United States)

    Bowie, Samuel; Alexeev, Alexander; Sulchek, Todd

    The understanding of dynamic cell behavior can aid in research ranging from the mechanistic causes of diseases to the development of microfluidic devices for cancer detection. Through analysis of trajectories captured from video of the cells moving in a specially designed microfluidic device, insight into the dynamic viscoelastic nature of cells can be found. The microfluidic device distinguishes cells viscoelastic properties through the use of angled ridges causing a series of compressions, resulting in differences in trajectories based on cell stiffness. Trajectories of cell passing through the device are collected using image processing methods and data mining techniques are used to relate the trajectories to cell properties obtained from experiments. Furthermore, numerical simulation of the cell and microfluidic device are used to match the experimental results from the trajectory analysis. Combination of the modeling and experimental data help to uncover how changes in cellular structures result in changes in mechanical properties.

  8. The Technical Efficiency of Departments of Shiraz Medical School in Research Arena Using Data Envelopment Analysis

    Directory of Open Access Journals (Sweden)

    Delavari Somaye

    2013-11-01

    Full Text Available Introduction: An efficient university should have a real goal and could produce the most output with the least use of inputs. Evaluation systems are an inseparable part of each organization and university. By efficiency calculation, useful information will be created about strengths, weaknesses, opportunities, threats, and opportunities for improvement. Data envelopment analysis is a useful technique for evaluation of efficiency. This technique needs all the inputs and outputs to be measured precisely.Methods and material: The present study is a descriptive, analytical, cross-sectional, and retrospective research which measures the research efficiency of educational departments of Shiraz faculty of medicine by using DEA technique from 2006 to 2011. Extracted data were analyzed by Microsoft excel and DEA master software.Finding: Department of pediatric and psychiatrics were efficient from 2006 to 2011. Other departments have some inefficiency. Neurosurgery department has least average of efficiency. The average of technical efficiency for all departments was 73.873.Conclusion: where the difference between efficiency score of the department and 100 is more, the department’s needs to improve its outputs will be more. Those groups which are more inefficient waste the resources more. This method also could help the faculty of medicine to identify their weakness and try for improvement

  9. Technical Analysis of Projects Being Funded by the DOE Hydrogen Program

    Energy Technology Data Exchange (ETDEWEB)

    Edward G. Skolnik

    2006-02-10

    In July 2000, Energetics began a project in which we performed site-visit based technical analyses or evaluations on hydrogen R&D projects for the purpose of providing in-depth information on the status and accomplishments of these projects to the public, and especially to hydrogen stakeholders. Over a three year period, 32 site-visit analyses were performed. In addition two concepts gleaned from the site visits became subjects of in depth techno-economic analyses. Finally, Energetics produced a compilation document that contains each site-visit analysis that we have performed, starting in 1996 on other contracts through the end of Year One of the current project (July 2001). This included 21 projects evaluated on previous contracts, and 10 additional ones from Year One. Reports on projects visited in Years One and Two were included in their respective Annual Reports. The Year Two Report also includes the two In-depth Analyses and the Compilation document. Reports in Year three began an attempt to perform reviews more geared to hydrogen safety. This Final Report contains a summary of the overall project, all of the 32 site-visit analyses and the two In-depth Analyses.

  10. Determining technical, allocative and cost efficiencies of rice farmers in Kedah, Malaysia using data envelopment analysis

    Science.gov (United States)

    Khan, Sahubar Ali Mohd. Nadhar; Baten, M. D. Azizul; Nawawi, Mohd. Kamal Mohd.; Murat, Rusdi @ Indra Zuhdi bin

    2016-10-01

    This study estimates technical, allocative, and cost efficiency using cost DEA model under both constant returns to scale (CRS) and variable returns to scale (VRS) respectively using survey data of 70 rice farmers from Kedah, Malaysia. In case of cost efficiency only 4.29% of the farmers were 100% technically efficient under CRS while it is increased into 16.90% under VRS. The average technical, allocative and cost efficiencies were estimated at 0.28, 0.878 and 0.255 respectively under CRS while they were increased into 0.61, 0.883 and 0.533 respectively under VRS.

  11. Investigating performance variability of processing, exploitation, and dissemination using a socio-technical systems analysis approach

    Science.gov (United States)

    Danczyk, Jennifer; Wollocko, Arthur; Farry, Michael; Voshell, Martin

    2016-05-01

    Data collection processes supporting Intelligence, Surveillance, and Reconnaissance (ISR) missions have recently undergone a technological transition accomplished by investment in sensor platforms. Various agencies have made these investments to increase the resolution, duration, and quality of data collection, to provide more relevant and recent data to warfighters. However, while sensor improvements have increased the volume of high-resolution data, they often fail to improve situational awareness and actionable intelligence for the warfighter because it lacks efficient Processing, Exploitation, and Dissemination and filtering methods for mission-relevant information needs. The volume of collected ISR data often overwhelms manual and automated processes in modern analysis enterprises, resulting in underexploited data, insufficient, or lack of answers to information requests. The outcome is a significant breakdown in the analytical workflow. To cope with this data overload, many intelligence organizations have sought to re-organize their general staffing requirements and workflows to enhance team communication and coordination, with hopes of exploiting as much high-value data as possible and understanding the value of actionable intelligence well before its relevance has passed. Through this effort we have taken a scholarly approach to this problem by studying the evolution of Processing, Exploitation, and Dissemination, with a specific focus on the Army's most recent evolutions using the Functional Resonance Analysis Method. This method investigates socio-technical processes by analyzing their intended functions and aspects to determine performance variabilities. Gaps are identified and recommendations about force structure and future R and D priorities to increase the throughput of the intelligence enterprise are discussed.

  12. Technical efficiency analysis of yam production in Edo state: A stochastic frontier approach

    Directory of Open Access Journals (Sweden)

    : Orewa, S.I.

    2012-09-01

    Full Text Available The technical efficiency of yam farmers in Edo State was analysed in this study using the Stochastic Frontier Production Model. A multistage sampling technique was employed to select the 180 yam farmers used for the study. A set of questionnaire was used to collect data from the respondents. Data collected were analysed using descriptive statistics and stochastic frontier production function. The result of the study revealed that the technical efficiency of the farmers range from 0.001 – 96.50% with a mean of 69.32%. This indicates ample opportunity for the farmers to increase their productivity through improvement in their technical efficiency. Farm size, yam sett, fertilizer and labour were found to be statistically significant and positively related to farmers output while educational level, household size and farming experience of the respondents negatively influenced farmers’ technical inefficiency. The farmers therefore need to increase their output through more intensive use of land, yam sett and fertilizer input.

  13. A polymeric micro total analysis system for single-cell analysis

    Science.gov (United States)

    Lai, Hsuan-Hong

    The advancement of microengineering has enabled the manipulation and analysis of single cells, which is critical in understanding the molecular mechanisms underlying the basic physiological functions from the point of view of modern biologists. Unfortunately, analysis of single cells remains challenging from a technical perspective, mainly because of the miniature nature of the cell and the high throughput requirements of the analysis. Lab-on-a-chip (LOC) emerges as a research field that shows great promise in this perspective. We have demonstrated a micro total analysis system (mu-TAS) combining chip-based electrophoretic separation, fluorescence detection, and a pulsed Nd:YAG laser cell lysis system, in a Poly(dimethylsiloxane) (PDMS) microfluidic analytical platform for the implementation of single-cell analysis. To accomplish the task, a polymeric microfluidic device was fabricated and UV graft polymerization surface modification techniques were used. To optimize the conditions for the surface treatment techniques, the modified surfaces of PDMS were characterized using AIR-IR spectrum and sessile water drop contact angle measurements, and in-channel surfaces were characterized by their electroosmotic flow mobility. Accurate single-cell analysis relies on rapid cell lysis and therefore an optical measure of fast cell lysis was implemented and optimized in a microscopic station. The influences of pulse energy and the location of the laser beam with respect to the cell in the microchannel were explored. The observation from the cell disruption experiments suggested that the cell lysis was enabled mainly via a thermo-mechanical instead of a plasma-mediated mechanism. Finally, after chip-based electrophoresis and a laser-induced fluorescence (LIF) detection system were incorporated with the laser lysis system in a microfluidic analytical station, a feasibility demonstration of single-cell analysis was implemented. The analytical platform exhibited the capability of

  14. Technical Versus Public Spheres: A Feminist Analysis of Women's Rhetoric in the Twilight Sleep Debates of 1914-1916.

    Science.gov (United States)

    Johnson, Bethany; Quinlan, Margaret M

    2015-01-01

    Twilight Sleep (TS) describes the delivery, via an injection, of an amnestic drug cocktail to a parturient woman throughout labor. In order to understand the development of modern-day rhetoric surrounding childbirth methods and procedures, this article explores the debate over TS between the public and technical sphere in New York City between 1914 and 1916 and examines the ways in which this debate altered obstetric health care for middle- and upper-class White women. The public response to this campaign posed a direct challenge to male obstetricians in New York City, many of whom were ill-equipped, both literally and figuratively, to use this procedure. Using a feminist rhetorical criticism, we examined the pro-TS rhetoric of women writers in New York City, the methods they borrowed from the women's movement, and the ensuing dialogue between the public and technical spheres. For this study, we analyzed journal and newspaper articles, a pamphlet, a collection of pro-TS organizational documents, letters to the editor, and books published about TS and the history of birth. Lastly, we analyzed theoretical notions of childbirth in women's health and communication studies. After examining the TS debate, we found that birth practices for middle- and upper-class women in New York City shifted and the obstetric community gained ascendancy over female midwifery. We also found that in certain instances, the rhetoric of pro-TS activists was more technically accurate than the rhetoric of some physicians. Hence the TS debate emerged from an argument over the right to use technical language in the technical and/or the public sphere. Conclusions and implications offered by this historical, feminist analysis question our current understanding of women's health and birthing practices, doctor-patient communication, and patient empowerment and access to technical knowledge.

  15. Comparative analysis of technical efficiency for different production culture systems and species of freshwater aquaculture in Peninsular Malaysia

    Directory of Open Access Journals (Sweden)

    Abdullahi Iliyasu

    2016-05-01

    Full Text Available This study estimated the bias-corrected technical efficiency (BCTE of different culture systems and species of freshwater aquaculture in Malaysia using bootstrapping data envelopment analysis (DEA. Data were collected from 307 respondents from three states in Peninsular Malaysia using a well-structured questionnaire as well as oral interviews. The findings indicate that all technical efficiency scores for all culture systems and species are below the optimal level (i.e. one. In addition, the results show that farmers’ experience, contact with extension workers and household size have a positive and statistically significant impact on technical efficiency. This implies that farmers who have long tenure in fish farming and also the opportunity to meet with extension workers are operating close to the production frontier (technically efficient. On the other hand, the age of the farmers has a negative and statistically significant impact on technical efficiency. Although educational level and farm status have a positive impact on technical efficiency, they are statistically insignificant. Furthermore, all the inputs used in the production process of different culture systems and species contained slacks and need to be reduced accordingly. Feed, the major input in fish production and constituting over half of the production costs, is equally over-utilized. Thus, the government, in collaboration with research institutes and universities, should design a feeding formula for fish depending on species, culture systems and stages of growth. This could help to reduce production costs, increasing the farmers' income, as well as providing much needed animal protein to consumers at an affordable rate.

  16. Technical and environmental analysis of biofuel dryers; Teknisk och miljoemaessig analys av biobraensletorkar

    Energy Technology Data Exchange (ETDEWEB)

    Muenter, M.; Hagman, U.; Harnevie, H.; Johansen, H.; Kristensson, I.; Westermark, M.; Viberg, T. [Vattenfall Energimarknad, Stockholm (Sweden)

    1999-03-01

    A study regarding technology and environmental impact of the drying process of biofuels has been conducted. In the study two different types of dryers are analyzed. The result from the study shows that emissions of primarily hydrocarbons is high from the direct technic. The emission can be decreased considerably by improving the burner of the dryer. For the indirect technic the evaporated water give rise to problems in the sewage treatment plant. Studies show that biological treatment will result in an acceptable condensate.

  17. Establishment and Analysis of Cancer Stem-Like and Non-Cancer Stem-Like Clone Cells from the Human Colon Cancer Cell Line SW480.

    Science.gov (United States)

    Takaya, Akari; Hirohashi, Yoshihiko; Murai, Aiko; Morita, Rena; Saijo, Hiroshi; Yamamoto, Eri; Kubo, Terufumi; Nakatsugawa, Munehide; Kanaseki, Takayuki; Tsukahara, Tomohide; Tamura, Yasuaki; Takemasa, Ichiro; Kondo, Toru; Sato, Noriyuki; Torigoe, Toshihiko

    2016-01-01

    Human cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) can be isolated as side population (SP) cells, aldehyde dehydrogenase high (ALDHhigh) cells or cell surface marker-positive cells including CD44+ cells and CD133+ cells. CSCs/CICs and non-CSCs/CICs are unstable in in vitro culture, and CSCs/CICs can differentiate into non-CSCs/CICs and some non-CSCs/CICs can dedifferentiate into CSCs/CICs. Therefore, experiments using a large amount of CSCs/CICs are technically very difficult. In this study, we isolated single cell clones from SP cells and main population (MP) cells derived from the human colon cancer cell line SW480. SP analysis revealed that SP clone cells had relatively high percentages of SP cells, whereas MP clone cells showed very few SP cells, and the phenotypes were sustainable for more than 2 months of in vitro culture. Xenograft transplantation revealed that SP clone cells have higher tumor-initiating ability than that of MP clone cells and SP clone cell showed higher chemo-resistance compared with MP clone cells. These results indicate that SP clone cells derived from SW480 cells are enriched with CSCs/CICs, whereas MP clone cells are pure non-CSCs/CICs. SP clone cells and MP clone cells are a very stable in vitro CSC/CIC-enriched and non-CSC/CIC model for further analysis.

  18. An analysis of Technical Security Control Requirements For Digital I and C Systems in Nuclear Power Plants

    Energy Technology Data Exchange (ETDEWEB)

    Song, Jaegu; Lee, Jungwoon; Park, Geeyong; Kwon, Keechoon; Lee, Dongyoung; Lee, Cheolkwon [Korea Atomic Energy Research Institute, Daejeon (Korea, Republic of)

    2013-10-15

    Instrumentation and control systems in nuclear power plants have been digitalized for the purpose of maintenance and precise operation. This digitalization, however, brings out issues related to cyber security. In the most recent past, international standard organizations, regulatory institutes, and research institutes have performed a number of studies addressing these systems cyber security. In order to provide information helpful to the system designers in their application of cyber security for the systems, this paper presents methods and considerations to define attack vectors in a target system, to review and select the requirements in the Regulatory Guide 5.71, and to integrate the results to identify applicable technical security control requirements. In this study, attack vectors are analyzed through the vulnerability analyses and penetration tests with a simplified safety system, and the elements of critical digital assets acting as attack vectors are identified. Among the security control requirements listed in Appendices B and C to Regulatory Guide 5.71, those that should be implemented into the systems are selected and classified in groups of technical security control requirements using the results of the attack vector analysis. For the attack vector elements of critical digital assets, all the technical security control requirements are evaluated to determine whether they are applicable and effective, and considerations in this evaluation are also discussed. The technical security control requirements in three important categories of access control, monitoring and logging, and encryption are derived and grouped according to the elements of attack vectors as results for the sample safety system.

  19. ANALYSIS AND EVALUATION OF VARIOUS SOFT SKILLS TRAINING MODELS EMPLOYED BY PRIVATE TECHNICAL EDUCATIONAL INSTITUTIONS IN INDIA

    Directory of Open Access Journals (Sweden)

    I. K. Kilam

    2013-07-01

    Full Text Available The growth of technical education has been unprecedented since the privatization of higher education in India. Almost all technical colleges attract students who meet the required eligibility criteria for enrolling in an engineering college, but unfortunately they are drastically deficient in soft skills and English language proficiency, which in turn makes these students unemployable, even after they complete their four years engineering degree course.Students who have a positive attitude, effective communication skills, problem solving ability, effective time management skills, team spirit, self-confidence, ability to handle criticism - skills that are also known as soft skills as a whole; have better chances of survival in the tough engineering and corporate world, compared to the students who are lacking in soft skillsThis paper offers to analyze the various aspects of Soft Skills Training Methods adopted by engineering colleges in India. Through analysis and evaluation of various models, the paper puts forward the best suited models for Indian technical colleges. The study is based on experience of imparting soft skills training in technical colleges, feedback from students and faculty members, study of models adopted across, study of Industry expectations and students’ requirement in India.

  20. From the Boom to the Collapse: a Technical Efficiency Analysis of the Spanish Construction Industry during the Financial Crisis

    Directory of Open Access Journals (Sweden)

    Xosé Luís Fernández-López

    2015-03-01

    Full Text Available Despite its contribution to the Spanish economy, as far as the authors are aware, the technical efficiency of the Spanish construction industry has neither been measured nor have the factors influencing it been analyzed. This paper measures the technical efficiency of the Spanish construction sector before and during the current financial crisis and investigates the degree to which factors influencing efficiency levels in this sector have changed. Stochastic frontier analysis (SFA methods are applied to firm-level data (692 constructions firms over the period 1996-2011. The results show that the average Technical Efficiency of the sector is 0.85. Results also indicate that variables affecting Technical Efficiency in the construction sector must be analyzed depending on real state cycle. Based on the findings, policy recommendations to improve the sector efficiency were developed. Important factors affecting efficiency change have been identified, and some managerial recommendations to increase the sector efficiency have been developed. The main recommendations for construction companies include: (1 cooperation and company mergers have a positive impact on companies’ efficiency; (2 accumulation of excessive financial burden damages the business long-term stability; and (3 business strategy should be tailored to economic prospects. Paper Type: Viewpoint

  1. DEMONSTRATION OF FUEL CELLS TO RECOVER ENERGY FROM LANDFILL GAS - PHASE III. DEMONSTRATION TESTS - PHASE IV. GUIDELINES AND RECOMMENDATIONS- VOLUME 1. TECHNICAL REPORT

    Science.gov (United States)

    The report summarizes the results of a four-phase program to demonstrate that fuel cell energy recovery using a commercial phosphoric acid fuel cell is both environmentally sound and commercially feasible. Phase I, a conceptual design and evaluation study, addressed the technical...

  2. [NASA/DOD Aerospace Knowledge Diffusion Research Project. Report 2:] Technical communications in aeronautics: Results of an exploratory study. An analysis of managers' and nonmanagers' responses

    Science.gov (United States)

    Pinelli, Thomas E.; Glassman, Myron; Barclay, Rebecca O.; Oliu, Walter E.

    1989-01-01

    Data collected from an exploratory study concerned with the technical communications practices of aerospace engineers and scientists were analyzed to test the primary assumption that aerospace managers and nonmanagers have different technical communications practices. Five assumptions were established for the analysis. Aerospace managers and nonmanagers were found to have different technical communications practices for three of the five assumptions tested. Although aerospace managers and nonmanagers were found to have different technical communications practices, the evidence was neither conclusive nor compelling that the presumption of difference in practices could be attributed to the duties performed by aerospace managers and nonmanagers.

  3. [NASA/DOD Aerospace Knowledge Diffusion Research Project. Report 3:] Technical communications in aeronautics: Results of an exploratory study. An analysis of profit managers' and nonprofit managers' responses

    Science.gov (United States)

    Pinelli, Thomas E.; Glassman, Myron; Barclay, Rebecca O.; Oliu, Walter E.

    1989-01-01

    Data collected from an exploratory study concerned with the technical communications practices of aerospace engineers and scientists were analyzed to test the primary assumption that profit and nonprofit managers in the aerospace community have different technical communications practices. Five assumptions were established for the analysis. Profit and nonprofit managers in the aerospace community were found to have different technical communications practices for one of the five assumptions tested. It was, therefore, concluded that profit and nonprofit managers in the aerospace community do not have different technical communications practices.

  4. A need analysis of technical writing skill of engineering students in India

    Directory of Open Access Journals (Sweden)

    Evangeline JCK

    2016-01-01

    Full Text Available The development of Science and Technology has bolstered the use of English Language around the world. The purpose of teaching English to non-native speakers of English is to help them in communicating, and more precisely sharing knowledge and information with speakers of other languages. In the field of higher education, the learners need English for Academic purposes (EAP and learn to use it later for occupational purposes (EOP. Students who had their primary education in English, manage to cope up with this way of the world; whereas students from sub-standard schools or vernacular medium of instruction, many times find it very difficult to cope up with the trend. This paper analyses the need to teach technical writing as a course to students of technical education in order to make them competent in academics and later in their work place. A few sample technical writing papers have been assessed and added to substantiate the need for educating students in technical writing skill. A review of literature and available ESP courses have been done to analyse the present strategies in teaching writing. A course was designed and tried to improve technical writing skill of students of higher education.

  5. Technical Data to Justify Full Burnup Credit in Criticality Safety Licensing Analysis

    Energy Technology Data Exchange (ETDEWEB)

    Enercon Services, Inc.

    2011-03-14

    Enercon Services, Inc. (ENERCON) was requested under Task Order No.2 to identify scientific and technical data needed to benchmark and justify Full Burnup Credit, which adds 16 fission products and 4 minor actinides1 to Actinide-Only burnup credit. The historical perspective for Full Burnup Credit is discussed, and interviews of organizations participating in burnup credit activities are summarized as a basis for identifying additional data needs and making recommendation. Input from burnup credit participants representing two segments of the commercial nuclear industry is provided. First, the Electric Power Research Institute (EPRI) has been very active in the development of Full Burnup Credit, representing the interests of nuclear utilities in achieving capacity gains for storage and transport casks. EPRI and its utility customers are interested in a swift resolution of the validation issues that are delaying the implementation of Full Burnup Credit [EPRI 2010b]. Second, used nuclear fuel storage and transportation Cask Vendors favor improving burnup credit beyond Actinide-Only burnup credit, although their discussion of specific burnup credit achievements and data needs was limited citing business sensitive and technical proprietary concerns. While Cask Vendor proprietary items are not specifically identified in this report, the needs of all nuclear industry participants are reflected in the conclusions and recommendations of this report. In addition, Oak Ridge National Laboratory (ORNL) and Sandia National Laboratory (SNL) were interviewed for their input into additional data needs to achieve Full Burnup Credit. ORNL was very open to discussions of Full Burnup Credit, with several telecoms and a visit by ENERCON to ORNL. For many years, ORNL has provided extensive support to the NRC regarding burnup credit in all of its forms. Discussions with ORNL focused on potential resolutions to the validation issues for the use of fission products. SNL was helpful in

  6. A technical study and analysis on fuzzy similarity based models for text classification

    CERN Document Server

    Puri, Shalini; 10.5121/ijdkp.2012.2201

    2012-01-01

    In this new and current era of technology, advancements and techniques, efficient and effective text document classification is becoming a challenging and highly required area to capably categorize text documents into mutually exclusive categories. Fuzzy similarity provides a way to find the similarity of features among various documents. In this paper, a technical review on various fuzzy similarity based models is given. These models are discussed and compared to frame out their use and necessity. A tour of different methodologies is provided which is based upon fuzzy similarity related concerns. It shows that how text and web documents are categorized efficiently into different categories. Various experimental results of these models are also discussed. The technical comparisons among each model's parameters are shown in the form of a 3-D chart. Such study and technical review provide a strong base of research work done on fuzzy similarity based text document categorization.

  7. Evaluating the effect of organization and context on technical efficiency: a second-stage DEA analysis of Italian hospitals

    Directory of Open Access Journals (Sweden)

    Domenica Matranga

    2013-10-01

    Full Text Available Objective: the purpose of this study was to compare the technical efficiency of Italian hospitals at a regional level and to examine if differences could be explained by organisational and contextual factors. Technical efficiency was defined as the ability of the operating units evaluated to use optimal resource levels for their level of output.Methods: the effect of external factors was explored through a second stage Data Envelopment Analysis (DEA. Efficiency scores were calculated for each hospital using the DEA method (Stage I. Through Tobit regression analysis, the estimated efficiency scores were regressed against a set of organisational and contextual characteristics beyond managerial control, which reflected differences in the population demographics and regional health expenditure (Stage II. Stage I and Stage II efficiency scores were compared in order to indirectly assess managerial contribution in relation to hospital efficiency.Results: the highest efficiency (M±SD was observed in hospitals in the North-West (75.7±15.1, followed by those in the North-East (75.5±15.1, Central Italy (73.9±16.4 and then Southern Italy (70.6±17.9. Hospital Trusts (HTs were shown to be more technically efficient than Local Public Hospitals (LPHs. Organisational and contextual indicators were statistically significantly different at Tobit regression analysis for HTs and LPHs. Emilia Romagna and Lombardia were the regions whose management contributed to increased efficiency.Conclusions: in our study, the distribution of regions according to technical efficiency only partly reflected the North-South gradient shown by other studies regarding the gap of expenditure. The important role of organisation and environment in establishing efficiency differences among hospitals was demonstrated. 

  8. Outcomes of Technical Variant Liver Transplantation versus Whole Liver Transplantation for Pediatric Patients: A Meta-Analysis.

    Directory of Open Access Journals (Sweden)

    Hui Ye

    Full Text Available To overcome the shortage of appropriate-sized whole liver grafts for children, technical variant liver transplantation has been practiced for decades. We perform a meta-analysis to compare the survival rates and incidence of surgical complications between pediatric whole liver transplantation and technical variant liver transplantation.To identify relevant studies up to January 2014, we searched PubMed/Medline, Embase, and Cochrane library databases. The primary outcomes measured were patient and graft survival rates, and the secondary outcomes were the incidence of surgical complications. The outcomes were pooled using a fixed-effects model or random-effects model.The one-year, three-year, five-year patient survival rates and one-year, three-year graft survival rates were significantly higher in whole liver transplantation than technical variant liver transplantation (OR = 1.62, 1.90, 1.65, 1.78, and 1.62, respectively, p<0.05. There was no significant difference in five-year graft survival rate between the two groups (OR = 1.47, p = 0.10. The incidence of portal vein thrombosis and biliary complications were significantly lower in the whole liver transplantation group (OR = 0.45 and 0.42, both p<0.05. The incidence of hepatic artery thrombosis was comparable between the two groups (OR = 1.21, p = 0.61.Pediatric whole liver transplantation is associated with better outcomes than technical variant liver transplantation. Continuing efforts should be made to minimize surgical complications to improve the outcomes of technical variant liver transplantation.

  9. FTIR Spectroscopic and Molecular Analysis during Differentiation of Pluripotent Stem Cells to Pancreatic Cells

    Directory of Open Access Journals (Sweden)

    Gustavo Jesus Vazquez-Zapien

    2016-01-01

    Full Text Available Some of the greatest challenges in stem cells (SCs biology and regenerative medicine are differentiation control of SCs and ensuring the purity of differentiated cells. In this work, we differentiated mouse pluripotent stem cells (mPSCs toward pancreatic cells characterizing this differentiation process by molecular and spectroscopic technics. Both mPSCs and Differentiated Pancreatic Cells (DPCs were subjected to a genetic, phenotypic, and biochemical analysis by real-time quantitative PCR (RT-qPCR, immunocytochemistry, and Fourier Transform Infrared (FTIR spectroscopy. Cultured mPCSs expressed pluripotent genes and proteins (Nanog and SOX2. DPCs expressed endodermal genes (SOX17 and Pdx1 at day 11, an inductor gene of embryonic pancreas development (Pdx1 at day 17 and pancreas genes and proteins (Insulin and Glucagon at day 21 of differentiation. Likewise, FTIR spectra of mPSCs and DPCs at different maturation stages (11, 17, and 21 days were obtained and showed absorption bands related with different types of biomolecules. These FTIR spectra exhibited significant spectral changes agreeing with the differentiation process, particularly in proteins and nucleic acids bands. In conclusion, the obtained DPCs passed through the chronological stages of embryonic pancreas development and FTIR spectra provide a new biophysical parameter based on molecular markers indicating the differentiation process of mPSCs to specialized cells.

  10. FTIR Spectroscopic and Molecular Analysis during Differentiation of Pluripotent Stem Cells to Pancreatic Cells

    Science.gov (United States)

    Mata-Miranda, Monica Maribel; Sanchez-Monroy, Virginia; Delgado-Macuil, Raul Jacobo; Perez-Ishiwara, David Guillermo

    2016-01-01

    Some of the greatest challenges in stem cells (SCs) biology and regenerative medicine are differentiation control of SCs and ensuring the purity of differentiated cells. In this work, we differentiated mouse pluripotent stem cells (mPSCs) toward pancreatic cells characterizing this differentiation process by molecular and spectroscopic technics. Both mPSCs and Differentiated Pancreatic Cells (DPCs) were subjected to a genetic, phenotypic, and biochemical analysis by real-time quantitative PCR (RT-qPCR), immunocytochemistry, and Fourier Transform Infrared (FTIR) spectroscopy. Cultured mPCSs expressed pluripotent genes and proteins (Nanog and SOX2). DPCs expressed endodermal genes (SOX17 and Pdx1) at day 11, an inductor gene of embryonic pancreas development (Pdx1) at day 17 and pancreas genes and proteins (Insulin and Glucagon) at day 21 of differentiation. Likewise, FTIR spectra of mPSCs and DPCs at different maturation stages (11, 17, and 21 days) were obtained and showed absorption bands related with different types of biomolecules. These FTIR spectra exhibited significant spectral changes agreeing with the differentiation process, particularly in proteins and nucleic acids bands. In conclusion, the obtained DPCs passed through the chronological stages of embryonic pancreas development and FTIR spectra provide a new biophysical parameter based on molecular markers indicating the differentiation process of mPSCs to specialized cells. PMID:27651798

  11. Lithium thionyl chloride cells and batteries Technical predictions versus 1994 realities

    Science.gov (United States)

    Staniewicz, R. J.

    Lithium thionyl chloride D-cells, when discharged at moderate rates of 50 W/kg, provide an impressive energy density of > 350 Wh/kg; however, multiple cell batteries present serious challenges for thermal management when subjected to discharge to 0 V and overdischarge into voltage reversal at the 50 W/kg rate. This paper describes the important influence electrochemical cell balance and design has upon decreasing the heat generation within batteries.

  12. Technical Training: Technical Training Seminar

    CERN Multimedia

    2004-01-01

    Tuesday 30 March TECHNICAL TRAINING SEMINAR From 9:00 to 12:00 and from 13:00 to 16:00 hrs - Council Chamber, Salle B, Salle des Pas Perdus National Instruments (NI) on Tour 2004 Claudia Jüngel, Evrem Yarkin, Joel Clerc, Hervé Baour / NATIONAL INSTRUMENTS The special event NI on Tour 2004, run in Germany, Austria and Switzerland, will be at CERN on March 30. Technical seminars and free introductory courses will be offered all day long in the Council Chamber, Salle B, and Salle des Pas Perdus (buildings 61 and 503). Technical conferences: 09:00 - 12:00 Data acquisition systems on PCs. Industrial measurement and control techniques. 13:00 - 16:00 Advanced LabVIEW software and PXI instrumentation. Measuring instruments and system components for teststand automation. Introductory courses: 09:00 - 12:00 DIAdem: Data analysis and presentation 13:00 - 16:00 Data acquisition with LabVIEW Language: English and French Free special seminar. Registration is recommended with National Instruments Switzerland (please sp...

  13. Technical Training: Technical Training Seminar

    CERN Multimedia

    2004-01-01

    Tuesday 30 March TECHNICAL TRAINING SEMINAR From 9:00 to 12:00 and from 13:00 to 16:00 hrs - Council Chamber, Salle B, Salle des Pas Perdus National Instruments (NI) on Tour 2004 Claudia Jüngel, Evrem Yarkin, Joel Clerc, Hervé Baour / NATIONAL INSTRUMENTS The special event NI on Tour 2004, run in Germany, Austria and Switzerland, will be at CERN on March 30. Technical seminars and free introductory courses will be offered all day long in the Council Chamber, Salle B, and Salle des Pas Perdus (buildings 61 and 503). Technical conferences: 09:00 - 12:00 Data acquisition systems on PCs. Industrial measurement and control techniques. 13:00 - 16:00 Advanced LabVIEW software and PXI instrumentation. Measuring instruments and system components for teststand automation. Introductory courses: 09:00 - 12:00 DIAdem: Data analysis and presentation 13:00 - 16:00 Data acquisition with LabVIEW Language: English and French Free special seminar. Registration is recommended with National Instruments Swi...

  14. Savremene metode analize ulja u tehničkim sistemima / Modern methods of oil analysis in technical systems

    Directory of Open Access Journals (Sweden)

    Sreten R. Perić

    2010-01-01

    Full Text Available Analiza ulja na osnovu pravilno definisanog programa predstavlja veoma efikasan metod monitoringa stanja tehničkih sistema koji obezbeđuje rane upozoravajuće znake potencijalnih problema, koji vode ka otkazu i zastoju tehničkih sistema. Ova analiza je veoma efikasan alat programa za monitoring stanja tehničkih sistema. Mnogobrojni uređaji i testovi za analizu ulja omogućavaju kvalitetan monitoring i dijagnosticiranje problema koji nastaju u procesu podmazivanja. Korišćenjem programa za analizu motornih ulja: skraćuje se neplanirano vreme otkaza vozila, poboljšava pouzdanost vozila, produžava radni vek motora, optimizira interval zamene ulja i smanjuju troškovi održavanja vozila. / Different technical systems require an appropriate lubricant to be used at an appropriate place, at appropriate time and in appropriate quantity. Determination of technical systems condition has a very important role in the development of theory and practice of friction, wear and lubrication. Lubricant is, as a contact element of tribomechanical systems, a carrier of information about the state of the whole system, from the aspect of tribological and other ageing processes. The analysis of oils, based on a properly defined program, thus represents a very effective method for monitoring the condition of technical systems, which ensures early warning signals of potential problems that could lead to failure and break down of technical systems. Introduction It is not always simple to determine a type of lubricant, frequency of lubrication and the quantity of lubricant to be used. The optimal recommendation would be to follow specifications of technical system manufacturers, experience, lab research or professional recommendation of lubricant suppliers. Rational lubricant consumption can be obtained by timely oil replacement, which then enables a maximum possible period of use as well as high-quality lubrication. Since the primary role of lubricants is to

  15. Isolation and analysis of mouse microglial cells.

    Science.gov (United States)

    Garcia, Jenny A; Cardona, Sandra M; Cardona, Astrid E

    2014-01-01

    Microglia are mononuclear phagocytes that make up about 10% of the central nervous system (CNS). They are known for their surveillant behavior, which involves continuous monitoring of neural tissue by extending and retracting their processes. Microglial cells are derived from myeloid progenitor cells and play important roles in homeostasis as well as inflammatory and immune responses in the brain. This unit describes several microglial cell isolation protocols that can be easily adapted for projects requiring a rapid and efficient analysis of mouse microglial cells by flow cytometry. Methods for visualizing microglial cells using in situ immunohistochemistry and immunochemistry in free-floating sections are also included.

  16. Fuel Cell-Powered Lift Truck Fleet Deployment Projects Final Technical Report May 2014

    Energy Technology Data Exchange (ETDEWEB)

    Klingler, James J [GENCO Infrastructure Solutions, Inc.

    2014-05-06

    The overall objectives of this project were to evaluate the performance, operability and safety of fork lift trucks powered by fuel cells in large distribution centers. This was accomplished by replacing the batteries in over 350 lift trucks with fuel cells at five distribution centers operated by GENCO. The annual cost savings of lift trucks powered by fuel cell power units was between $2,400 and $5,300 per truck compared to battery powered lift trucks, excluding DOE contributions. The greatest savings were in fueling labor costs where a fuel cell powered lift truck could be fueled in a few minutes per day compared to over an hour for battery powered lift trucks which required removal and replacement of batteries. Lift truck operators where generally very satisfied with the performance of the fuel cell power units, primarily because there was no reduction in power over the duration of a shift as experienced with battery powered lift trucks. The operators also appreciated the fast and easy fueling compared to the effort and potential risk of injury associated with switching heavy batteries in and out of lift trucks. There were no safety issues with the fueling or operation of the fuel cells. Although maintenance costs for the fuel cells were higher than for batteries, these costs are expected to decrease significantly in the next generation of fuel cells, making them even more cost effective.

  17. Recent technical advances in thin-film CdS/CdTe solar cells

    Energy Technology Data Exchange (ETDEWEB)

    Omura, K.; Hanahusa, A.; Arita, T.; Higuchi, H.; Aramoto, T.; Nishio, T.; Sibutani, S.; Kumazawa, S.; Murozono, M. [Matsushita Battery Industrial Co., Ltd., Osaka (Japan). PV Research and Development Center; Yabuuchi, Y. [Matsushita Technoresearch Inc., Osaka (Japan); Takakura, H. [Toyama Prefectural University, Toyama (Japan)

    1996-05-01

    CeS/CdTe solar cells have attracted attention recently for their potential as low-cost, high-efficiency solar cells of the future. It is because the CdTe layer (used for photoelectric conversion) has a bandgap energy of 1.51 eV, which corresponds well to sunlight spectra, and the direct transition type energy band structure enables formation of thinner films. We have already industrialized CdS/CdTe solar cells in mass production stage using a printing-sintering process, as large-area modules for electric power generation (Higuchi et al., 1993, Omura et al., 1991), and as cells for indoor applications (primarily in calculators, Suyama et al., 1986). However, this solar cell has a conversion efficiency of approximately 6%. Recently, there has been considerable research into thin-film CdS/CdTe solar cells which have a thinner CdS film formed by CVD or CBD (Britt et al., 1993) process, and thus are photosensitive to light with wavelengths of 500 nm or less. At present stage of our art, in solar cells formed by the CSS with a CdTe film on CVD CdS, a conversion efficiency of 15.05% has been obtained in cells with an area of 1 cm{sup 2}(verified at JQA). (author)

  18. Analysis of Anther Cell Differentiation

    Energy Technology Data Exchange (ETDEWEB)

    Ma, Hong [Pennsylvania State Univ., University Park, PA (United States)

    2015-01-19

    This grant supports research on genes that regulate Arabidopsis anther development. The proposed research largely concerns that functions of two key regulatory genes: SPL and DYT1, which encode two putative transcription factors, as well as genes that interact with these genes. Last year, we reported progress in preparation for ChIP analysis with SPL and DYT1, in dyt1 and ams microarray experiments and initial data analysis, in functional analysis of one of the DYT1 target gene, MYB35.

  19. A Socio-Technical Analysis of Web Content Management: "Content is King."

    Science.gov (United States)

    Eschenfelder, Kristin

    2001-01-01

    Examines how ongoing content management needs and tasks affect organizations that maintain Web-based information systems (Web IS). Investigates how organizational context shapes content management practice and configuration of the underlying Web IS technology. Presents a model of the socio-technical context of Web IS content management. (AEF)

  20. 77 FR 47495 - Final Priority; Technical Assistance on State Data Collection, Analysis, and Reporting-National...

    Science.gov (United States)

    2012-08-08

    ... and 618 of IDEA, which may include the development of open source data system software that addresses... Relay Service (FRS), toll free, at 1- 800-877-8339. SUPPLEMENTARY INFORMATION: Purpose of Program: The... records. For the Privacy Technical Assistance Center Help Desk, email PrivacyTA@ed.gov or call, toll...

  1. Analysis of Influencing Factors of Technical Barriers on Information Product Exports Based on the Fuzzy AHP

    Institute of Scientific and Technical Information of China (English)

    YuyingWu; NaLi

    2004-01-01

    To the actual situation of TBT impacting information product and according to the concept of the triangular fuzzy number, this paper forms the fuzzy matrix of factors of impacting export of information product, then uses the fuzzy AHP to analyze and rate factors. We put forward suggestions on how to keep away and surpass the technical barriers to trade in the information product enterprises.

  2. Success and failure of technical analysis in the cocoa futures market

    NARCIS (Netherlands)

    Boswijk, P.; Griffioen, G.; Hommes, C.; Kyrtsou, C.; Vorlow, C.

    2012-01-01

    A large set of 5350 trend following technical trading rules is applied to LIFFE and CSCE cocoa futures prices, and to the Pound-Dollar exchange rate, in the period 1983:1-1997:6. We find that 58% of the trading rules generates a strictly positive excess return, even when correcting for transaction c

  3. Analysis of GB 6675-2003 National Safety Technical Code for Toys in Detail

    Institute of Scientific and Technical Information of China (English)

    2005-01-01

    @@ Ⅰ Introduction GB 6675-2003 National Safety Technical Code for Toys, which is a compulsory national standard and developed in accordance with the Standardization Law of P. R. China and the Product Quality Law ofP. R. China,has been officially issued on Oct. 9th, 2003.

  4. Web-Conferencing: An Analysis of Course Delivery Systems on Student Achievement at a Technical College

    Science.gov (United States)

    Stanford, Roger John

    2012-01-01

    Web-conferencing software was chosen for course delivery to provide flexible options for students at a two-year technical college. Students used technology to access a live, synchronous microeconomics course over the internet instead of a traditional face-to-face lecture. This investigation studied the impact of implementing web-conferencing…

  5. Removal of trace organics from water by pervaporation : a technical and economic analysis

    NARCIS (Netherlands)

    Nijhuis, Herman Henk

    1990-01-01

    Volatile organic hydrocarbons in ground water, surface water or waste water are a continuous threat to the public health. Often traditional purification techniques cannot be applied to remove these components from aqueous solutions. In this thesis the technical and economic feasibility of pervaporat

  6. The Hepaticojejunostomy Technique with Intra-Anastomotic Stent in Biliary Diseases and Its Evolution throughout the Years: A Technical Analysis

    Directory of Open Access Journals (Sweden)

    Demetrios Moris

    2016-01-01

    Full Text Available Roux-en-Y hepaticojejunostomy (RYHJ is currently considered as the definitive treatment for iatrogenic bile duct injuries and the principal representative of biliary diversion procedures. This technique has met many milestones of extensive evolution, particularly the last years of concomitant technological evolution (laparoscopic/robotic approach. Anastomotic strictures and leaks, which may have deleterious effects on the survival and quality of life of a patient with biliary obstruction of any cause, made the need of the development of a safe and efficient RYHJ compulsory. The aim of this technical analysis and the juxtaposed discussions is to elucidate with the most important milestones and technical tips and tricks all aspects of a feasible and reliable RYHJ technique that is performed in our center for the last 25 years in around 400 patients.

  7. Using data envelopment analysis to measure the extent of technical efficiency of public health centres in Ghana

    Directory of Open Access Journals (Sweden)

    Jehu-Appiah Caroline

    2008-11-01

    Full Text Available Abstract Background Data Envelopment Analysis (DEA has been used to analyze the efficiency of the health sector in the developed world for sometime now. However, in developing economies and particularly in Africa only a few studies have applied DEA in measuring the efficiency of their health care systems. Methods This study uses the DEA method, to calculate the technical efficiency of 89 randomly sampled health centers in Ghana. The aim was to determine the degree of efficiency of health centers and recommend performance targets for the inefficient facilities. Results The findings showed that 65% of health centers were technically inefficient and so were using resources that they did not actually need. Conclusion The results broadly point to grave inefficiency in the health care delivery system of public health centers and that significant amounts of resources could be saved if measures were put in place to curb the waste.

  8. CT-guided Bipolar and Multipolar Radiofrequency Ablation (RF Ablation) of Renal Cell Carcinoma: Specific Technical Aspects and Clinical Results

    Energy Technology Data Exchange (ETDEWEB)

    Sommer, C. M., E-mail: christof.sommer@med.uni-heidelberg.de [University Hospital Heidelberg, INF 110, Department of Diagnostic and Interventional Radiology (Germany); Lemm, G.; Hohenstein, E. [Minimally Invasive Therapies and Nuclear Medicine, SLK Kliniken Heilbronn GmbH, Clinic for Radiology (Germany); Bellemann, N.; Stampfl, U. [University Hospital Heidelberg, INF 110, Department of Diagnostic and Interventional Radiology (Germany); Goezen, A. S.; Rassweiler, J. [Clinic for Urology, SLK Kliniken Heilbronn GmbH (Germany); Kauczor, H. U.; Radeleff, B. A. [University Hospital Heidelberg, INF 110, Department of Diagnostic and Interventional Radiology (Germany); Pereira, P. L. [Minimally Invasive Therapies and Nuclear Medicine, SLK Kliniken Heilbronn GmbH, Clinic for Radiology (Germany)

    2013-06-15

    Purpose. This study was designed to evaluate the clinical efficacy of CT-guided bipolar and multipolar radiofrequency ablation (RF ablation) of renal cell carcinoma (RCC) and to analyze specific technical aspects between both technologies. Methods. We included 22 consecutive patients (3 women; age 74.2 {+-} 8.6 years) after 28 CT-guided bipolar or multipolar RF ablations of 28 RCCs (diameter 2.5 {+-} 0.8 cm). Procedures were performed with a commercially available RF system (Celon AG Olympus, Berlin, Germany). Technical aspects of RF ablation procedures (ablation mode [bipolar or multipolar], number of applicators and ablation cycles, overall ablation time and deployed energy, and technical success rate) were analyzed. Clinical results (local recurrence-free survival and local tumor control rate, renal function [glomerular filtration rate (GFR)]) and complication rates were evaluated. Results. Bipolar RF ablation was performed in 12 procedures and multipolar RF ablation in 16 procedures (2 applicators in 14 procedures and 3 applicators in 2 procedures). One ablation cycle was performed in 15 procedures and two ablation cycles in 13 procedures. Overall ablation time and deployed energy were 35.0 {+-} 13.6 min and 43.7 {+-} 17.9 kJ. Technical success rate was 100 %. Major and minor complication rates were 4 and 14 %. At an imaging follow-up of 15.2 {+-} 8.8 months, local recurrence-free survival was 14.4 {+-} 8.8 months and local tumor control rate was 93 %. GFR did not deteriorate after RF ablation (50.8 {+-} 16.6 ml/min/1.73 m{sup 2} before RF ablation vs. 47.2 {+-} 11.9 ml/min/1.73 m{sup 2} after RF ablation; not significant). Conclusions. CT-guided bipolar and multipolar RF ablation of RCC has a high rate of clinical success and low complication rates. At short-term follow-up, clinical efficacy is high without deterioration of the renal function.

  9. Culture medium modulates the behaviour of human dental pulp-derived cells: Technical Note

    Directory of Open Access Journals (Sweden)

    S Lopez-Cazaux

    2006-02-01

    Full Text Available In vitro approaches have extensively been developed to study reparative dentinogenesis. While dental pulp is a source of unidentified progenitors able to differentiate into odontoblast-like cells, we investigated the effect of two media; MEM (1.8mM Ca and 1mM Pi and RPMI 1640 (0.8mM Ca and 5mM Pi on the behaviour of human dental pulp cells. Our data indicate that MEM significantly increased cell proliferation and markedly enhanced the proportion of -smooth muscle actin positive cells, which represent a putative source of progenitors able to give rise to odontoblast-like cells. In addition, MEM strongly stimulated alkaline phosphatase activity and was found to induce expression of transcripts encoding dentin sialophosphoprotein, an odontoblastic marker, without affecting that of parathyroid hormone/parathyroid hormone related protein-receptor and osteonectin. In conclusion, these observations demonstrate that not only proliferation but also differentiation into odontoblast-like cells was induced by rich calcium and poor phosphate medium (MEM as compared to RPMI 1640. This study provides important data for the determination of the optimal culture conditions allowing odontoblast-like differentiation in human pulp cell culture.

  10. Single-cell analysis - Methods and protocols

    OpenAIRE

    Carlo Alberto Redi

    2013-01-01

    This is certainly a timely volume in the Methods in molecular biology series: we already entered the synthetic biology era and thus we need to be aware of the new methodological advances able to fulfill the new and necessary needs for biologists, biotechnologists and nano-biotechnologists. Notably, among these, the possibility to perform single cell analysis allow researchers to capture single cell responses....

  11. Special Analysis: Disposal Plan for Pit 38 at Technical Area 54, Area G

    Energy Technology Data Exchange (ETDEWEB)

    French, Sean B. [Los Alamos National Laboratory; Shuman, Rob [URS Coporation

    2012-06-26

    Los Alamos National Laboratory (LANL) generates radioactive waste as a result of various activities. Operational waste is generated from a wide variety of research and development activities including nuclear weapons development, energy production, and medical research; environmental restoration (ER), and decontamination and decommissioning (D&D) waste is generated as contaminated sites and facilities at LANL undergo cleanup or remediation. The majority of this waste is low-level radioactive waste (LLW) and is disposed of at the Technical Area 54 (TA-54), Area G disposal facility. U.S. Department of Energy (DOE) Order 435.1 (DOE, 2001) requires that radioactive waste be managed in a manner that protects public health and safety, and the environment. To comply with this order, DOE field sites must prepare site-specific radiological performance assessments for LLW disposal facilities that accept waste after September 26, 1988. Furthermore, sites are required to conduct composite analyses that account for the cumulative impacts of all waste that has been (or will be) disposed of at the facilities and other sources of radioactive material that may interact with the facilities. Revision 4 of the Area G performance assessment and composite analysis was issued in 2008 (LANL, 2008). These analyses estimate rates of radionuclide release from the waste disposed of at the facility, simulate the movement of radionuclides through the environment, and project potential radiation doses to humans for several on- and off-site exposure scenarios. The assessments are based on existing site and disposal facility data, and on assumptions about future rates and methods of waste disposal. The Area G disposal facility consists of Material Disposal Area (MDA) G and the Zone 4 expansion area. To date, disposal operations have been confined to MDA G and are scheduled to continue in that region until MDA G undergoes final closure at the end of 2013. Given its impending closure, efforts have

  12. Silicon Based Solid Oxide Fuel Cell Chip for Portable Consumer Electronics -- Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Alan Ludwiszewski

    2009-06-29

    LSI’s fuel cell uses efficient Solid Oxide Fuel Cell (“SOFC”) technology, is manufactured using Micro Electrical Mechanical System (“MEMS”) fabrication methods, and runs on high energy fuels, such as butane and ethanol. The company’s Fuel Cell on a Chip™ technology enables a form-factor battery replacement for portable electronic devices that has the potential to provide an order-of-magnitude run-time improvement over current batteries. Further, the technology is clean and environmentally-friendly. This Department of Energy funded project focused on accelerating the commercialization and market introduction of this technology through improvements in fuel cell chip power output, lifetime, and manufacturability.

  13. Nonchromosomal cytogenetic analysis of mammal somatic cells

    Directory of Open Access Journals (Sweden)

    Kovalova O. А.

    2013-01-01

    Full Text Available The mutational events that take place in mammalian somatic cells influenced with different endogenous and exogenous factors are presented in this review. The nonchromosomal method of research allows taking into account the complex cell characteristics without time-consuming analysis of the chromosomes as such. As a result, the information can be obtained about the mitotic (phases of mitosis, the number of nuclei per cell, micronuclei, pathology of mitosis and vital (mitotic index, apoptosis cell statuses, as well as about the state of chromosomal integrity (the presence of nucleoplasmic bridges, nucleus protrusions, chromosome fragmentation, micronuclei. Depending on the material studied (erythrocytes and lymphocytes of peripheral blood, buccal cells, permanent cell lines etc., a complex of cytogenetic characteristics can be selected for each case which is the most informative for determination of the mutational spectra in mammalian somatic cells.

  14. Final Technical Report, Oct 2004 - Nov. 2006, High Performance Flexible Reversible Solid Oxide Fuel Cell

    Energy Technology Data Exchange (ETDEWEB)

    Guan, Jie; Minh, Nguyen

    2007-02-21

    This report summarizes the work performed for the program entitled “High Performance Flexible Reversible Solid Oxide Fuel Cell” under Cooperative Agreement DE-FC36-04GO14351 for the U. S. Department of Energy. The overall objective of this project is to demonstrate a single modular stack that generates electricity from a variety of fuels (hydrogen and other fuels such as biomass, distributed natural gas, etc.) and when operated in the reverse mode, produces hydrogen from steam. This project has evaluated and selected baseline cell materials, developed a set of materials for oxygen and hydrogen electrodes, and optimized electrode microstructures for reversible solid oxide fuel cells (RSOFCs); and demonstrated the feasibility and operation of a RSOFC multi-cell stack. A 10-cell reversible SOFC stack was operated over 1000 hours alternating between fuel cell (with hydrogen and methane as fuel) and steam electrolysis modes. The stack ran very successfully with high power density of 480 mW/cm2 at 0.7V and 80% fuel utilization in fuel cell mode and >6 SLPM hydrogen production in steam electrolysis mode using about 1.1 kW electrical power. The hydrogen generation is equivalent to a specific capability of 2.59 Nm3/m2 with electrical energy demand of 3 kWh/Nm3. The performance stability in electrolysis mode was improved vastly during the program with a degradation rate reduction from 8000 to 200 mohm-cm2/1000 hrs. This was accomplished by increasing the activity and improving microstructure of the oxygen electrode. Both cost estimate and technology assessment were conducted. Besides the flexibility running under both fuel cell mode and electrolysis mode, the reversible SOFC system has the potentials for low cost and high efficient hydrogen production through steam electrolysis. The cost for hydrogen production at large scale was estimated at ~$2.7/kg H2, comparing favorably with other electrolysis techology.

  15. A socio-technical analysis of work with ideas in NPD: an industrial case study

    DEFF Research Database (Denmark)

    Gish, Liv; Hansen, Claus Thorp

    2013-01-01

    The work with ideas is perceived by both academics and industry to be an important element in new product development and has thus been the subject of investigation in many different research fields. In this paper, we investigate a specific case showing how a successful product was developed based...... on piecing together a number of ideas that were developed and disseminated in a large industrial company. We do this through an in-depth case study of the development of the energy-labeled circulation pump Alpha Pro, developed by one of the world’s leading pump manufacturers, Grundfos. Using a socio......-technical approach, we focus especially on the actors involved and the contextual factors, and less on the detailed development of technical ideas. In our study, we observe that (1) ideas are pieced together from previous ideas and results; (2) ideas are implemented through continuous mobilization of support...

  16. Empirical Analysis on the Impacts of Technical Barrier to Trade on Chinese Agro-products Export

    Institute of Scientific and Technical Information of China (English)

    2011-01-01

    In view of the impacts on Chinese agro-products export caused by various technical barriers to trade,the academic circles analyzed them from the costs and quantity of exported agro-products.On the basis of the status quo of Chinese agro-products,the gravity model is introduced to analyze the impacts on Chinese agro-product export caused by technical barriers to trade.The results show that as long as the quality of Chinese agro-products can achieve the standard set by developed countries,for one thing,the health of Chinese consumers can be ensured,for another thing,the export of Chinese agro-products will be more smooth,which can provide reference for our government to make decisions and solve trade dispute.

  17. ANALYSIS OF THE POSSIBILITY OF INTEGRATING A MINING RIGHT-ANGLE PLANETARY GEARBOX WITH TECHNICAL DIAGNOSTICS SYSTEMS

    Directory of Open Access Journals (Sweden)

    Andrzej WIECZOREK

    2016-12-01

    Full Text Available A key factor enabling the achievement of the required capacity by longwall mining systems is to obtain a satisfactory service life for individual components of such systems. Such components include right-angle planetary gearboxes for armoured face conveyors. An increase in the service life of such equipment can be achieved by ensuring adequacy in terms of design, materials and organization. As a part of organizational changes, the use of individual diagnostics systems may have the greatest impact on the service life of mining gearboxes; however, their widespread implementation is limited by economic and operational barriers. This paper presents an analysis of the possibility of integrating mining gearboxes with electronic systems of technical diagnostics, as well as expanding the scope of the technical condition monitoring by the machines operating together with these gearboxes. As a result of the calculation and design work performed, it has been demonstrated that it is possible to integrate technical diagnostics systems with advanced data transmission capabilities inside gearboxes.

  18. Direct methanol fuel cells for transportation applications. Quarterly technical report, June 1996--September 1996

    Energy Technology Data Exchange (ETDEWEB)

    Fuller, T.F.; Kunz, H.R.; Moore, R.

    1996-11-01

    The purpose of this research and development effort is to advance the performance and viability of direct methanol fuel cell technology for light-duty transportation applications. For fuel cells to be an attractive alternative to conventional automotive power plants, the fuel cell stack combined with the fuel processor and ancillary systems must be competitive in terms of both performance and costs. A major advantage for the direct methanol fuel cell is that a fuel processor is not required. A direct methanol fuel cell has the potential of satisfying the demanding requirements for transportation applications, such as rapid start-up and rapid refueling. The preliminary goals of this effort are: (1) 310 W/l, (2) 445 W/kg, and (3) potential manufacturing costs of $48/kW. In the twelve month period for phase 1, the following critical areas will be investigated: (1) an improved proton-exchange membrane that is more impermeable to methanol, (2) improved cathode catalysts, and (3) advanced anode catalysts. In addition, these components will be combined to form membrane-electrode assemblies (MEA`s) and evaluated in subscale tests. Finally a conceptual design and program plan will be developed for the construction of a 5 kW direct methanol stack in phase II of the program.

  19. TECHNICAL ANALYSIS OF FUZZY METAGRAPH BASED DECISION SUPPORT SYSTEM FOR CAPITAL MARKET

    OpenAIRE

    Anbalagan Thirunavukarasu; Uma Maheswari

    2013-01-01

    This study proposes a Fuzzy Metagraph based Decision Support System (DSS) for short term and long term investment in share market. This rule base decision system will help traders to make correct decision at very low risk. Relative Strength Index (RSI), Moving Average Convergence Divergence (MACD) and WILLIAM- %R are some of the Technical Indicators which are used as input to train the system which is integrated with Fuzzy Metagraph. This approach of incorporating Fuzzy Metagraph with RSI, MA...

  20. Micro and nanoplatforms for biological cell analysis

    DEFF Research Database (Denmark)

    Svendsen, Winnie Edith; Castillo, Jaime; Moresco, Jacob Lange

    2010-01-01

    In this paper some of the technological platforms developed in our group for biological cell analysis will be highlighted. The paper first presents a short introduction pinpointing the advantages of using micro and nano technology in cellular studies. The issues of requiring transient analysis...

  1. Technical and environmental efifciency of hog production in China-A stochastic frontier production function analysis

    Institute of Scientific and Technical Information of China (English)

    ZHOU Ying-heng; ZHANG Xiao-heng; TIAN Xu; GENG Xian-hui; ZHANG Peng; YAN Bin-jian

    2015-01-01

    This article analyses the technical and environmental efifciency of hog production in China using data from the China Agricultural Product Cost-Beneift Compilation (NDRC 2005–2013) and the First National Census of Pol ution:Manual of Discharge Coefifcient of Livestock and Poultry Industry (IEDA and NIES 2009). The empirical results show a great variation in environmental efifciency, ranging from 0.344 to 0.973 with a mean value of 0.672 that declines over time. Southwest China is found to be the most environmental y efifcient region, while the Northeast and the Northwest are the least efifcient. Another ifnding is that technical and environmental efifciencies are highly correlated in hog production;the most environ-mental y efifcient regions are usual y found to have high technical efifciency, and vice versa. In addition, we computed the output elasticities with respect to each factor input. The results show that feed is the most efifcient input, with an output elasticity of approximately 0.551, which is much higher than the elasticity of the nitrogen surplus, other capital or labour. The output elasticity with respect to the nitrogen surplus is 0.287 on average. Final y, the scale elasticity in hog production is slightly higher than 1.

  2. AN ANALYSIS OF TECHNICAL SECURITY CONTROL REQUIREMENTS FOR DIGITAL I&C SYSTEMS IN NUCLEAR POWER PLANTS

    Directory of Open Access Journals (Sweden)

    JAE-GU SONG

    2013-10-01

    Full Text Available Instrumentation and control systems in nuclear power plants have been digitalized for the purpose of maintenance and precise operation. This digitalization, however, brings out issues related to cyber security. In the most recent past, international standard organizations, regulatory institutes, and research institutes have performed a number of studies addressing these systems cyber security. In order to provide information helpful to the system designers in their application of cyber security for the systems, this paper presents methods and considerations to define attack vectors in a target system, to review and select the requirements in the Regulatory Guide 5.71, and to integrate the results to identify applicable technical security control requirements. In this study, attack vectors are analyzed through the vulnerability analyses and penetration tests with a simplified safety system, and the elements of critical digital assets acting as attack vectors are identified. Among the security control requirements listed in Appendices B and C to Regulatory Guide 5.71, those that should be implemented into the systems are selected and classified in groups of technical security control requirements using the results of the attack vector analysis. For the attack vector elements of critical digital assets, all the technical security control requirements are evaluated to determine whether they are applicable and effective, and considerations in this evaluation are also discussed. The technical security control requirements in three important categories of access control, monitoring and logging, and encryption are derived and grouped according to the elements of attack vectors as results for the sample safety system.

  3. Technical and scale efficiency in the delivery of child health services in Zambia: results from data envelopment analysis

    Science.gov (United States)

    Achoki, Tom; Hovels, Anke; Masiye, Felix; Lesego, Abaleng; Leufkens, Hubert; Kinfu, Yohannes

    2017-01-01

    Objective Despite tremendous efforts to scale up key maternal and child health interventions in Zambia, progress has not been uniform across the country. This raises fundamental health system performance questions that require further investigation. Our study investigates technical and scale efficiency (SE) in the delivery of maternal and child health services in the country. Setting The study focused on all 72 health districts of Zambia. Methods We compiled a district-level database comprising health outcomes (measured by the probability of survival to 5 years of age), health outputs (measured by coverage of key health interventions) and a set of health system inputs, namely, financial resources and human resources for health, for the year 2010. We used data envelopment analysis to assess the performance of subnational units across Zambia with respect to technical and SE, controlling for environmental factors that are beyond the control of health system decision makers. Results Nationally, average technical efficiency with respect to improving child survival was 61.5% (95% CI 58.2% to 64.8%), which suggests that there is a huge inefficiency in resource use in the country and the potential to expand services without injecting additional resources into the system. Districts that were more urbanised and had a higher proportion of educated women were more technically efficient. Improved cooking methods and donor funding had no significant effect on efficiency. Conclusions With the pressing need to accelerate progress in population health, decision makers must seek efficient ways to deliver services to achieve universal health coverage. Understanding the factors that drive performance and seeking ways to enhance efficiency offer a practical pathway through which low-income countries could improve population health without necessarily seeking additional resources. PMID:28057650

  4. Final Technical Report: Effects of Impurities on Fuel Cell Performance and Durability

    Energy Technology Data Exchange (ETDEWEB)

    James G. Goodwin, Jr.; Hector Colon-Mercado; Kitiya Hongsirikarn; and Jack Z. Zhang

    2011-11-11

    The main objectives of this project were to investigate the effect of a series of potential impurities on fuel cell operation and on the particular components of the fuel cell MEA, to propose (where possible) mechanism(s) by which these impurities affected fuel cell performance, and to suggest strategies for minimizing these impurity effects. The negative effect on Pt/C was to decrease hydrogen surface coverage and hydrogen activation at fuel cell conditions. The negative effect on Nafion components was to decrease proton conductivity, primarily by replacing/reacting with the protons on the Bronsted acid sites of the Nafion. Even though already well known as fuel cell poisons, the effects of CO and NH3 were studied in great detail early on in the project in order to develop methodology for evaluating poisoning effects in general, to help establish reproducibility of results among a number of laboratories in the U.S. investigating impurity effects, and to help establish lower limit standards for impurities during hydrogen production for fuel cell utilization. New methodologies developed included (1) a means to measure hydrogen surface concentration on the Pt catalyst (HDSAP) before and after exposure to impurities, (2) a way to predict conductivity of a Nafion membranes exposed to impurities using a characteristic acid catalyzed reaction (methanol esterification of acetic acid), and, more importantly, (3) application of the latter technique to predict conductivity on Nafion in the catalyst layer of the MEA. H2-D2 exchange was found to be suitable for predicting hydrogen activation of Pt catalysts. The Nafion (ca. 30 wt%) on the Pt/C catalyst resides primarily on the external surface of the C support where it blocks significant numbers of micropores, but only partially blocks the pore openings of the meso- and macro-pores wherein lie the small Pt particles (crystallites). For this reason, even with 30 wt% Nafion on the Pt/C, few Pt sites are blocked and, hence, are

  5. Enzymology and Molecular Biology of Cell Wall Biosynthesis. Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Dr. Peter M. Ray

    2000-04-01

    The following aspects of enzymology of cell wall synthesis were pursued under this cited grant: (1) Isolation of plasma membrane-localized glucan synthase II (GS-II) of pea; (2) Cloning of genes for possible plant GS-II components; (3) Golgi glucan synthase-I (GS-I); and (4) Golgi reversibly glycosylated protein 1 (RGP1).

  6. Analysis of Aviation Safety Reporting System Incident Data Associated With the Technical Challenges of the Vehicle Systems Safety Technology Project

    Science.gov (United States)

    Withrow, Colleen A.; Reveley, Mary S.

    2014-01-01

    This analysis was conducted to support the Vehicle Systems Safety Technology (VSST) Project of the Aviation Safety Program (AVsP) milestone VSST4.2.1.01, "Identification of VSST-Related Trends." In particular, this is a review of incident data from the NASA Aviation Safety Reporting System (ASRS). The following three VSST-related technical challenges (TCs) were the focus of the incidents searched in the ASRS database: (1) Vechicle health assurance, (2) Effective crew-system interactions and decisions in all conditions; and (3) Aircraft loss of control prevention, mitigation, and recovery.

  7. Final Technical Report: Effects of Impurities on Fuel Cell Performance and Durability

    Energy Technology Data Exchange (ETDEWEB)

    James G. Goodwin, Jr.; Hector Colon-Mercado; Kitiya Hongsirikarn; and Jack Z. Zhang

    2011-11-11

    The main objectives of this project were to investigate the effect of a series of potential impurities on fuel cell operation and on the particular components of the fuel cell MEA, to propose (where possible) mechanism(s) by which these impurities affected fuel cell performance, and to suggest strategies for minimizing these impurity effects. The negative effect on Pt/C was to decrease hydrogen surface coverage and hydrogen activation at fuel cell conditions. The negative effect on Nafion components was to decrease proton conductivity, primarily by replacing/reacting with the protons on the Bronsted acid sites of the Nafion. Even though already well known as fuel cell poisons, the effects of CO and NH3 were studied in great detail early on in the project in order to develop methodology for evaluating poisoning effects in general, to help establish reproducibility of results among a number of laboratories in the U.S. investigating impurity effects, and to help establish lower limit standards for impurities during hydrogen production for fuel cell utilization. New methodologies developed included (1) a means to measure hydrogen surface concentration on the Pt catalyst (HDSAP) before and after exposure to impurities, (2) a way to predict conductivity of a Nafion membranes exposed to impurities using a characteristic acid catalyzed reaction (methanol esterification of acetic acid), and, more importantly, (3) application of the latter technique to predict conductivity on Nafion in the catalyst layer of the MEA. H2-D2 exchange was found to be suitable for predicting hydrogen activation of Pt catalysts. The Nafion (ca. 30 wt%) on the Pt/C catalyst resides primarily on the external surface of the C support where it blocks significant numbers of micropores, but only partially blocks the pore openings of the meso- and macro-pores wherein lie the small Pt particles (crystallites). For this reason, even with 30 wt% Nafion on the Pt/C, few Pt sites are blocked and, hence, are

  8. Final Technical Report Microwave Assisted Electrolyte Cell for Primary Aluminum Production

    Energy Technology Data Exchange (ETDEWEB)

    Xiaodi Huang; J.Y. Hwang

    2007-04-18

    This research addresses the high priority research need for developing inert anode and wetted cathode technology, as defined in the Aluminum Industry Technology Roadmap and Inert Anode Roadmap, with the performance targets: a) significantly reducing the energy intensity of aluminum production, b) ultimately eliminating anode-related CO2 emissions, and c) reducing aluminum production costs. This research intended to develop a new electrometallurgical extraction technology by introducing microwave irradiation into the current electrolytic cells for primary aluminum production. This technology aimed at accelerating the alumina electrolysis reduction rate and lowering the aluminum production temperature, coupled with the uses of nickel based superalloy inert anode, nickel based superalloy wetted cathode, and modified salt electrolyte. Michigan Technological University, collaborating with Cober Electronic and Century Aluminum, conducted bench-scale research for evaluation of this technology. This research included three sub-topics: a) fluoride microwave absorption; b) microwave assisted electrolytic cell design and fabrication; and c) aluminum electrowinning tests using the microwave assisted electrolytic cell. This research concludes that the typically used fluoride compound for aluminum electrowinning is not a good microwave absorbing material at room temperature. However, it becomes an excellent microwave absorbing material above 550°C. The electrowinning tests did not show benefit to introduce microwave irradiation into the electrolytic cell. The experiments revealed that the nickel-based superalloy is not suitable for use as a cathode material; although it wets with molten aluminum, it causes severe reaction with molten aluminum. In the anode experiments, the chosen superalloy did not meet corrosion resistance requirements. A nicked based alloy without iron content could be further investigated.

  9. [Hydroxyproline: Rich glycoproteins of the plant and cell wall]. Annual technical progress report, 1993

    Energy Technology Data Exchange (ETDEWEB)

    Varner, J.E.

    1993-06-01

    Since xylem tissue includes the main cell types which are lignified, we are interested in gene expression of glycine-rich proteins and proline-rich proteins, and other proteins which are involved in secondary cell wall thickening during xylogenesis. Since the main feature of xylogenesis is the deposition of additional wall components, study of the mechanism of xylogenesis will greatly advance our knowledge of the synthesis and assembly of wall macromolecules. We are using the in vitro xylogenesis system from isolated Zinnia mesophyll cells to isolate genes which are specifically expressed during xylogenesis. We have used subtractive hybridization methods to isolate a number of cDNA clones for differentially regulated genes from the cells after hormonal induction. So far, we have partially characterized 18 different cDNA clones from 239 positive clones. These differentially regulated genes can be divided into three sets according to the characteristics of gene expression in the induction medium and the control medium. The first set is induced in both the induction medium and the control medium without hormones. The second set is induced mainly in the induction medium and in the control medium with the addition of NAA alone. Two of thesegenes are exclusively induced by auxin. The third set of genes is induced mainly in the induction medium. Since these genes are not induced by either auxin or cytokinin alone, they may be directly involved in the process of xylogenesis. Our experiments on the localization of H{sub 2}O{sub 2} production reinforce the earlier ideas of others that H{sub 2}O{sub 2} is involved in normal lignification.

  10. Final Technical Report CONDUCTIVE COATINGS FOR SOLAR CELLS USING CARBON NANOTUBES

    Energy Technology Data Exchange (ETDEWEB)

    Paul J Glatkowski; Jorma Peltola; Christopher Weeks; Mike Trottier; David Britz

    2007-09-30

    US Department of Energy (DOE) awarded a grant for Eikos Inc. to investigate the feasibility of developing and utilizing Transparent Conducting Coatings (TCCs) based on carbon nanotubes (CNT) for solar cell applications. Conventional solar cells today employ metal oxide based TCCs with both Electrical Resistivity (R) and Optical Transparency (T), commonly referred to as optoelectronic (RT) performance significantly higher than with those possible with CNT based TCCs available today. Transparent metal oxide based coatings are also inherently brittle requiring high temperature in vacuum processing and are thus expensive to manufacture. One such material is indium tin oxide (ITO). Global demand for indium has recently increased rapidly while supply has diminished causing substantial spikes in raw material cost and availability. In contrast, the raw material, carbon, needed for CNT fabrication is abundantly available. Transparent Conducting Coatings based on CNTs can overcome not only cost and availability constraints while also offering the ability to be applied by existing, low cost process technologies under ambient conditions. Processes thus can readily be designed both for rigid and flexible PV technology platforms based on mature spray or dip coatings for silicon based solar cells and continuous roll to roll coating processes for polymer solar applications.

  11. Cell tracking using {sup 19}F magnetic resonance imaging: Technical aspects and challenges towards clinical applications

    Energy Technology Data Exchange (ETDEWEB)

    Amiri, Houshang [Radboud University Medical Center, Department of Radiology, Nijmegen (Netherlands); Radboud University Medical Center, Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Nijmegen (Netherlands); Srinivas, Mangala; Vries, I.J.M. de [Radboud University Medical Center, Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Nijmegen (Netherlands); Veltien, Andor; Uden, Mark J. van; Heerschap, Arend [Radboud University Medical Center, Department of Radiology, Nijmegen (Netherlands)

    2014-11-06

    {sup 19}F MRI is emerging as a new imaging technique for cell tracking. It is particularly attractive because of its potential for direct and precise cell quantification. The most important challenge towards in vivo applications is the sensitivity of the technique, i.e. the detection limit in a reasonable imaging time. Optimal sensitivity can be achieved with dedicated {sup 19}F compounds together with specifically adapted hardware and acquisition methods. In this paper we introduce the {sup 19}F MRI technique focusing on these key sensitivity issues and review the state-of-the-art of {sup 19}F MRI and developments towards its clinical use. We calculate {sup 19}F detection limits reported in preclinical cell and clinical {sup 19}F drug studies in terms of tissue concentration in a 1 cm{sup 3} voxel, as an alternate way to compare detection limits. We estimate that a tissue concentration of a few millimoles per litre (mM) of {sup 19}F is required for a human study at a resolution of 1 cm{sup 3}. (orig.)

  12. Self-Sealing Wet Chemistry Cell for Field Analysis

    Science.gov (United States)

    Beegle, Luther W.; Soto, Juancarlos; Lasnik, James; Roark, Shane

    2012-01-01

    In most analytical investigations, there is a need to process complex field samples for the unique detection of analytes, especially when detecting low concentration organic molecules that may identify extraterrestrial life. Wet chemistry based instruments are the techniques of choice for most laboratory- based analysis of organic molecules due to several factors including less fragmentation of fragile biomarkers, and ability to concentrate target species resulting in much lower limits of detection. Development of an automated wet chemistry preparation system that can operate autonomously on Earth and is also designed to operate under Martian ambient conditions will demonstrate the technical feasibility of including wet chemistry on future missions. An Automated Sample Processing System (ASPS) has recently been developed that receives fines, extracts organics through solvent extraction, processes the extract by removing non-organic soluble species, and delivers sample to multiple instruments for analysis (including for non-organic soluble species). The key to this system is a sample cell that can autonomously function under field conditions. As a result, a self-sealing sample cell was developed that can autonomously hermetically seal fines and powder into a container, regardless of orientation of the apparatus. The cap is designed with a beveled edge, which allows the cap to be self-righted as the capping motor engages. Each cap consists of a C-clip lock ring below a crucible O-ring that is placed into a groove cut into the sample cap.

  13. On a nonlinear partial differential algebraic system arising in technical textile industry: Analysis and numerics

    CERN Document Server

    Grothaus, Martin

    2012-01-01

    In this paper a length-conserving numerical scheme for a nonlinear fourth order system of partial differential algebraic equations arising in technical textile industry is studied. Applying a semidiscretization in time, the resulting sequence of nonlinear elliptic systems with algebraic constraint is reformulated as constrained optimization problems in a Hilbert space setting that admit a solution at each time level. Stability and convergence of the scheme are proved. The numerical realization is performed by projected gradient methods on finite element spaces which determine the computational effort and approximation quality of the algorithm. Simulation results are presented and discussed in view of the application of an elastic inextensible fiber motion.

  14. Technical foundations of TANDEM-STRAIGHT, a speech analysis, modification and synthesis framework

    Indian Academy of Sciences (India)

    Hideki Kawahara; Masanori Morise

    2011-10-01

    This article presents comprehensive technical information about STRAIGHT and TANDEM-STRAIGHT, a widely used speech modification tool and its successor. They share the same concept: the periodic excitation found in voiced sounds is an efficient mechanism for transmitting underlying smooth time–frequency representation. The tools are also based on the perceptual equivalence of two sets of independent Gaussian random signals. This equivalence makes it possible to discard input phase information intentionally and enables flexible manipulation of parameters.

  15. Technical analysis of US Army Weapons Systems and related advanced technologies of military interest. Final report

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    1991-06-14

    This report summarizes the activities and accomplishments of an US Army technology security project designed to identify and develop effective policy guidelines for militarily critical technologies in specific Army systems and in broad generic technology areas of military interest, Individual systems analyses are documented in separate Weapons Systems Technical Assessments (WSTAs) and the general generic technology areas are evaluated in the Advanced Technology Assessment Reports (ATARs), However, specific details of these assessments are not addressed here, only recommendations regarding aspects of the defined approach, methodology, and format are provided and discussed.

  16. Fluorescence-Activated Cell Sorting Analysis of Heterotypic Cell-in-Cell Structures.

    Science.gov (United States)

    He, Meifang; Huang, Hongyan; Wang, Manna; Chen, Ang; Ning, Xiangkai; Yu, Kaitao; Li, Qihong; Li, Wen; Ma, Li; Chen, Zhaolie; Wang, Xiaoning; Sun, Qiang

    2015-04-27

    Cell-in-cell structures (CICs), characterized by the presence of one or more viable cells inside another one, were recently found important player in development, immune homeostasis and tumorigenesis etc. Incompatible with ever-increasing interests on this unique phenomenon, reliable methods available for high throughput quantification and systemic investigation are lacking. Here, we report a flow cytometry-based method for rapid analysis and sorting of heterotypic CICs formed between lymphocytes and tumor cells. In this method, cells were labeled with fluorescent dyes for fluorescence-activated cell sorting (FACS) by flow cytometry, conditions for reducing cell doublets were optimized such that high purity (>95%) of CICs could be achieved. By taking advantage of this method, we analyzed CICs formation between different cell pairs, and found that factors from both internalized effector cells and engulfing target cells affect heterotypic CICs formation. Thus, flow cytometry-based FACS analysis would serve as a high throughput method to promote systemic researches on CICs.

  17. Single-cell analysis - Methods and protocols

    Directory of Open Access Journals (Sweden)

    Carlo Alberto Redi

    2013-06-01

    Full Text Available This is certainly a timely volume in the Methods in molecular biology series: we already entered the synthetic biology era and thus we need to be aware of the new methodological advances able to fulfill the new and necessary needs for biologists, biotechnologists and nano-biotechnologists. Notably, among these, the possibility to perform single cell analysis allow researchers to capture single cell responses....

  18. Cell Specific eQTL Analysis without Sorting Cells.

    Directory of Open Access Journals (Sweden)

    Harm-Jan Westra

    2015-05-01

    Full Text Available The functional consequences of trait associated SNPs are often investigated using expression quantitative trait locus (eQTL mapping. While trait-associated variants may operate in a cell-type specific manner, eQTL datasets for such cell-types may not always be available. We performed a genome-environment interaction (GxE meta-analysis on data from 5,683 samples to infer the cell type specificity of whole blood cis-eQTLs. We demonstrate that this method is able to predict neutrophil and lymphocyte specific cis-eQTLs and replicate these predictions in independent cell-type specific datasets. Finally, we show that SNPs associated with Crohn's disease preferentially affect gene expression within neutrophils, including the archetypal NOD2 locus.

  19. TECHNICAL ANALYSIS OF FUZZY METAGRAPH BASED DECISION SUPPORT SYSTEM FOR CAPITAL MARKET

    Directory of Open Access Journals (Sweden)

    Anbalagan Thirunavukarasu

    2013-01-01

    Full Text Available This study proposes a Fuzzy Metagraph based Decision Support System (DSS for short term and long term investment in share market. This rule base decision system will help traders to make correct decision at very low risk. Relative Strength Index (RSI, Moving Average Convergence Divergence (MACD and WILLIAM- %R are some of the Technical Indicators which are used as input to train the system which is integrated with Fuzzy Metagraph. This approach of incorporating Fuzzy Metagraph with RSI, MACD and WILLIAM-%R would be a new attempt in decision making on share market investment. Stocks listed in Bombay Stock Exchange (BSE in India are used to evaluate the performance of the system. The results obtained from the proposed Fuzzy Metagraph based model are found to be satisfactory with very low risk. Three most used technical indicators MACD, RSI and WILLIAM-%R integrated with Fuzzy Metagraph are used to support the system. This method reduces the risk factor considerably for both short term and long term investors.

  20. Puget Sound Area Electric Reliability Plan : Scoping Summary Report - Part B Preliminary Technical Analysis Appendix A.

    Energy Technology Data Exchange (ETDEWEB)

    United States. Bonneville Power Administration.

    1991-09-01

    This report describes in general terms the nature of the voltage instability problem facing the Puget Sound area. The following two chapters cover the technical aspects of the problem. It deals with load growth, the root cause of the problem. Also addressed is the capacity of the current power system and the criteria for future system planning. It also explains the technical results of transmission system modeling which confirm the system's vulnerability to voltage instability, the principal symptom of the problem. The results of the scoping process in each of the four measure categories are presented. Included are lists of all options identified, a discussion of the screening criteria, and descriptions of the measures that survived the screening process and are proposed for further evaluation in Phase 2. We discuss the evaluation methodology which will be used to refine the analyses. The next steps in the planning process are outlined. It also describes the short term operational agreements that will assure continued reliable service until a long term solution is in place. 8 figs., 22 tabs.

  1. Technical and harmonic analysis of Carl Czerny op. 299 number 34 etude

    Directory of Open Access Journals (Sweden)

    Mehmet Serkan Umuzdas

    2012-11-01

    Full Text Available In this study, the 34th etude of the book Czerny Op. 299 that is one of the commonly employed books in the piano training was analysed in terms of technique and harmony. The etude was examined in terms of its technical features and contributions to technical development. If an etude is analysed before it is played, time and effort can be amanged much more efficiently. In turn, it may contribute to play the etude or work in accordance with its objectives and to produce outcomes. The aims of this study are to make the students aware of the goals and methods of etudes and to provide them with the suggestions for studying. It is suggested that any etude written with the 2/4 rhythm pattern should be played very vividly and energytically. Any etude written in the octave width of 5.5 is made up of 43 scales in two section. The etude is composed of two sections, each with four sentences and two periods. It also involves 43 scales. Of them, 16 scales are in the first section and the remaining 27 scales are in the second section. The etude has very regular system in terms of harmonic continuity and motives. It has a homogenious pattern in terms of the order of the sentences with half-decsion and those with full-decision.

  2. Imatinib Case: Technical and Legal Analysis of Patent Prosecution in Colombia

    Directory of Open Access Journals (Sweden)

    Luisa Fernanda Díaz Pinilla

    2016-12-01

    Full Text Available Imatinib –marketed as Gleevec™ or Glivec™– is a drug mainly used to treat a condition called Acute myeloid leukemia (AML. Its patent protection in Colombia, as a polymorphic entity of mesylate salt –called Beta polymorphous–, exhibits a divergent procedure due to differences between Colombian Patent Office (SIC and State Council judgment given in order to deny patent right in 2003 and then revoking such denial and ordering patent grant in 2012, respectively. This paper evaluates technical regards associated with each institution arguments and legal topics considered to conclude the instance. Findings show that studies carried out in relation to testimonial evidence and expert advice –that were submitted by two experts –one of them– directly related to the patent application– guided Colombian State Council in order to grant the polymorphous patent, even when there was no clear evidence of inventive step, especially referred as a compound surprising technical effect. Furthermore, the paper includes some comparisons related to legal instances that are in charge of studying patent office decisions in other jurisdictions and protection given –or not– to polymorphous entities in other countries.

  3. Fingertip replantation: Technical considerations and outcome analysis of 24 consecutive fingertip replantations

    Directory of Open Access Journals (Sweden)

    H Venkatramani

    2011-01-01

    Full Text Available Fingertip amputations are one of the most common injuries faced in an emergency department. Finger tip replantation though technically possible, are not regularly done due to the presumed complexity of the procedure and doubts about the outcome. This article deals with our experience of 24 fingertip replantations in 24 patients done over a period of 8 years since the year 2000. Twenty-one fingertips survived. The most common affected digit in the series was thumb followed by index, middle, and ring. The overall success rate was 87%. Both arterial and venous repair were done in all cases. Replantation was not done if no suitable vein was found for anastomosis. Nine patients did not have nerve repair. Seven of them survived and all of them had satisfactory sensation when examined after 1 year. No patient suffered from cold intolerance. All patients were satisfied with the functional outcome and aesthetic appearance. This article highlights the technical considerations and the outcome of these fingertip replants.

  4. Analysis of China's Renewable Energy Development under the Current Economic and Technical Circumstances

    Institute of Scientific and Technical Information of China (English)

    Dan Shi

    2009-01-01

    At present, the development of renewable energy relies mainly on government support. The government invests in a considerable number of projects to improve public welfare and to assist in poverty relief. If China is to replace fossil fuels on a large scale with renewable energy sources, the production costs und prices of renewable energy must he brought down. All countries are facing the challenge of moving to a more secure and low-carbon energy system without weakening economic and social development. In this regard, China is facing an even greater challenge in terms of economic cost, as cheap coal remains the main energy form. Technical innovation and industrialization in the area of renewable energy is an important means of lowering cost. China is in for a period of high-speed development of its economy and the rising demand for energy is irreversible. If the technical progress and development speed of renewable energy lags behind the growth in demand, it will be difficult to realize the improvement of its energy structure.

  5. Analysis of Vertical Architecture Photogalvanic Cells

    CERN Document Server

    Mahmoudzadeh, Mohammad Ali

    2013-01-01

    A new configuration of the photogalvanic cells (PGCs) is proposed that relaxes some of the hard to achieve requirements for efficient conventional photogalvanic cells. Instead of illuminating the device perpendicular to the electrode as was done classically, the vertical alignment of PGCs is suggested so that the light comes in from the gap between the two electrodes. This way, the electron generation extends to the depth of the device and therefore slower kinetics are allowed for deep cells. The depth can also be adjusted according to the concentration of the dyes, which solves the high solubility problem of the electrode-illuminated cells. The configuration is mathematically modeled and the advantages over the conventional cell are shown. A computer model is built for more detailed analysis.

  6. Microfabricated devices for single cell analysis

    Science.gov (United States)

    Gao, Yuanfang

    BioMEMS or lab-on-a-chip technology is promising technology and enables the possibility of microchip devices with higher throughput or better performance for single cell analysis. We have designed and fabricated microdevices for single cell analysis, with impedance based device for fast cell screening and microchannel based flow systems for high throughput, high time resolution quantal exocytosis measurement with automatic cell positioning and reusability. The automatic cell positioning is realized by differential forces of fluidic dynamics. Microelectrodes are patterned at automatic trap positions for electrochemical detection quantal release of hormones like catecholamines secreted by cells. We also developed diamond-like carbon (DLC) microelectrodes onto chip device for low noise exocytosis measurement. The DLC microelectrodes were deposited by magnetron sputtering process with nitrogen doping and a bottom ITO conductive layer. Test results show the developed DLC can detect exocytosis with low noise and a stable background current which are comparable to that of carbon-fiber electrodes. They are batch producible at low cost and can realize high-throughput on-chip measurement of quantal exocytosis. The technology developed in this research can have wide ranging applications in fields such as electrophysiology, cell based sensors, high throughput screening of new drug development.

  7. Laser interstitial thermal therapy for subependymal giant cell astrocytoma: technical case report.

    Science.gov (United States)

    Dadey, David Y A; Kamath, Ashwin A; Leuthardt, Eric C; Smyth, Matthew D

    2016-10-01

    Subependymal giant cell astrocytoma (SEGA) is a rare tumor occurring almost exclusively in patients with tuberous sclerosis complex. Although open resection remains the standard therapy, complication rates remain high. To minimize morbidity, less invasive approaches, such as endoscope-assisted resection, radiosurgery, and chemotherapy with mTOR pathway inhibitors, are also used to treat these lesions. Laser interstitial thermal therapy (LITT) is a relatively new modality that is increasingly used to treat a variety of intracranial lesions. In this report, the authors describe two pediatric cases of SEGA that were treated with LITT. In both patients the lesion responded well to this treatment modality, with tumor shrinkage observed on follow-up MRI. These cases highlight the potential of LITT to serve as a viable minimally invasive therapeutic approach to the management of SEGAs in the pediatric population.

  8. The hegemonic construction of technical institutions in the Argentine agriculture: analysis of AAPRESID and AACREA speeches in the last decade

    Directory of Open Access Journals (Sweden)

    María Dolores Liaudat Landivar

    2015-08-01

    Full Text Available This paper seeks to research on a type of organizations that gains relevance in recent decades in the Argentine agricultural world: the "technical organizations". We intend to trace the ideological nature and the aim of hegemonic construction of the two most representative associations: AAPRESID and AACREA. To advance in this way of research we have worked on the analysis of their discourses, using as sources documents the institutional journals, publications and videos, and minutes of the annual conferences. From this analysis we trace three elements: the roots of local and transnational discourses that they promote, the ways to articulate them and the hegemonic construction operations. These elements allow us to show the strategy of AAPRESID and AACREA to become an intellectual and moral authority in our country.

  9. Texas Hydrogen Highway Fuel Cell Hybrid Bus and Fueling Infrastructure Technology Showcase - Final Scientific/Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Hitchcock, David

    2012-06-29

    The Texas Hydrogen Highway project has showcased a hydrogen fuel cell transit bus and hydrogen fueling infrastructure that was designed and built through previous support from various public and private sector entities. The aim of this project has been to increase awareness among transit agencies and other public entities on these transportation technologies, and to place such technologies into commercial applications, such as a public transit agency. The initial project concept developed in 2004 was to show that a skid-mounted, fully-integrated, factory-built and tested hydrogen fueling station could be used to simplify the design, and lower the cost of fueling infrastructure for fuel cell vehicles. The approach was to design, engineer, build, and test the integrated fueling station at the factory then install it at a site that offered educational and technical resources and provide an opportunity to showcase both the fueling station and advanced hydrogen vehicles. The two primary technology components include: Hydrogen Fueling Station: The hydrogen fueling infrastructure was designed and built by Gas Technology Institute primarily through a funding grant from the Texas Commission on Environmental Quality. It includes hydrogen production, clean-up, compression, storage, and dispensing. The station consists of a steam methane reformer, gas clean-up system, gas compressor and 48 kilograms of hydrogen storage capacity for dispensing at 5000 psig. The station is skid-mounted for easy installation and can be relocated if needed. It includes a dispenser that is designed to provide temperaturecompensated fills using a control algorithm. The total station daily capacity is approximately 50 kilograms. Fuel Cell Bus: The transit passenger bus built by Ebus, a company located in Downey, CA, was commissioned and acquired by GTI prior to this project. It is a fuel cell plug-in hybrid electric vehicle which is ADA compliant, has air conditioning sufficient for Texas operations

  10. Technical, perceptual and motor skills in novice-expert water polo players: an individual discriminant analysis for talent development.

    Science.gov (United States)

    DʼErcole, Alessandro A; DʼErcole, Cristina; Gobbi, Massimo; Gobbi, Fabio

    2013-12-01

    The 4 tasks (A, B, C, and Y) have the characteristic of containing one more element than the task performed before it. In fact, task B introduces the slalom which is not present in task A. Task C introduces the ball control that are not present in tasks A and B, whereas task Y introduces the slalom and ball control in a visual dual task situation developed in horizontal swimming over a distance of 20 m at maximum speed. This exercise not included in task C. These tasks were performed by a group of pre-adolescent players and national under 18 water polo players. The novice players showed that tasks B and C are predictors of task Y. Such characteristics were not present in the expert players. The novice players also had difficulty in performing task Y because of the visual-attention overload, a difficulty that was not present in the expert players. To improve the 4 skills, the coach of the novice players developed a technical-didactic program, which was checked 6 months after the pretest. The posttest was not significantly different from the pretest while the individual discriminant analysis identified the improvements in some novice players, which on elaboration proved significant, enabling us to distinguish 2 subgroups, one with higher learning rates and the other with lower learning rates. In the practical applications, we describe the didactic tools (task analysis) and the different levels of development of technical skills in water polo. Improvements in these skills are explained through computational models like the HMOSAIC (Hierarchical, Modular, Selection and Identification for Control) while the individual discriminant analysis enables us to do a longitudinal analysis that is not possible with cross-sectional models.

  11. Monochromosomal hybrids for the analysis of the human genome. Final technical report

    Energy Technology Data Exchange (ETDEWEB)

    Athwal, R.S. [Temple Univ. School of Medicine, Philadelphia, PA (United States). Fels Inst. for Cancer Research and Molecular Biology

    1994-09-01

    The objective of this research project is to produce panels of mouse/human and/or Chinese hamster/human hybrid cell lines each harboring a single different human chromosome. The human chromosome present in rodent cell will be marked with a dominant selectable marker and maintained by selection. In these experiments human chromosomes first ``tagged`` with a selectable marker in human cells are subsequently transferred to rodent cells by microcell fusion method. Several different experimental schemes have been developed to ``tag`` human chromosomes with a selectable marker. Amphotropic retroviral vectors provide a highly efficient system to introduce selectable markers into normal diploid human cells. The integration of retroviral vector into the cell genome occurs at random by recombination at a defined nucleotide sequence in the LTRs and only a single copy of the vector integrates in a cell. This property of retroviral vectors allows to isolate a segment of the chromosomal DNA flanking the vector integration site by PCR amplification. In these studies the amphotropic retroviral vector pZIPgpt that carries a dominant selectable marker gpt, is used to tag the human chromosomes in normal diploid cells. Human DNA flanking the integrated vector is rescued by PCR amplification and cloned into a plasmid vector. Cloned human DNA is then used to probe Southern blots of DNAs from a panel of hybrid cell lines to identify the chromosome of its origin. This allows them to identify clonal human cell lines, each carrying the marker integrated into a different chromosome. Marked chromosomes are then transferred to rodent cells by MMCT.

  12. Technical analysis and optimization of a high concentrating photovoltaic power tower

    Science.gov (United States)

    Moreno, Karolina Ordóñez; Heimsath, Anna; Wiesenfarth, Maike; Schöttl, Peter; Nitz, Peter

    2015-09-01

    Based on the results of previous research, a technical study on a 1 MW prototype photovoltaic power tower is presented in this contribution. Some options to optimize the heliostat field were assessed to improve the optical efficiency and yearly energy output. Aiming strategies to create a highly homogeneous radiant flux distribution on the PV receiver and additional optimization options were analyzed. A significant improvement compared to earlier results and an annual mean optical efficiency of 54.7 % was achieved. The cost calculation for the improved system resulted in levelized cost of electricity (LCOE) of 0.19 €/kWh for the exemplary location Seville, Spain. Assumptions for further improvements and heliostat field cost reduction showed an additional cost reduction potential to 0.08 €/kWh.

  13. A technical and economic analysis of one potential pathway to a 100% renewable energy system

    DEFF Research Database (Denmark)

    Connolly, David; Mathiesen, Brian Vad

    2014-01-01

    requirements, 5) adding flexible electricity demands and electric vehicles, 6) producing synthetic methanol/DME for transport, and finally 7) using synthetic gas to replace the remaining fossil fuels. For each stage, the technical and economic performance of the energy system is calculated. The results...... indicate that a 100% renewable energy system can provide the same end-user energy demands as today’s energy system and at the same price. Electricity will be the backbone of the energy system, but the flexibility in today’s electricity sector will be transferred from the supply side of the demand side...... in the future. Similarly, due to changes in the type of spending required in a 100% renewable energy system, this scenario will result in the creation of 100,000 additional jobs in Ireland compared to an energy system like today’s. These results are significant since they indicate that the transition to a 100...

  14. Economical, environmental and technical analysis of building integrated photovoltaic systems in Malaysia

    Energy Technology Data Exchange (ETDEWEB)

    Seng, Lim Yun [Department of Physical Science, Electrical and Electronic Engineering, Tunku Abdul Rahman University, 53300 Setapak, Kuala Lumpur (Malaysia); Lalchand, G.; Sow Lin, Gladys Mak [Malaysia Energy Centre, Building Integrated Photovoltaic Project (Malaysia)

    2008-06-15

    Malaysia has identified photovoltaic systems as one of the most promising renewable sources. A great deal of efforts has been undertaken to promote the wide applications of PV systems. With the recent launch of a PV market induction programme known as SURIA 1000 in conjunction with other relevant activities undertaken under the national project of Malaysia Building Integrated Photovoltaic (MBIPV), the market of PV systems begins to be stimulated in the country. As a result, a wide range of technical, environmental and economic issues with regard to the connection of PV systems to local distribution networks becomes apparent. Numerous studies were therefore carried out in collaboration with Malaysian Energy Centre to address a number of those important issues. The findings of the studies are presented in the paper and can be served as supplementary information to parties who are directly and indirectly involved in the PV sector in Malaysia. (author)

  15. Computer Simulation of Technetium Scrubbing Section of Purex Ⅰ: Computer Simulation and Technical Parameter Analysis

    Institute of Scientific and Technical Information of China (English)

    CHEN; Yan-xin; HE; Hui; ZHANG; Chun-long; CHANG; Li; LI; Rui-xue; TANG; Hong-bin; YU; Ting

    2012-01-01

    <正>A computer program was developed to simulate technetium scrubbing section (TcS) in Purex based on the theory of cascade extraction. The program can simulate the steady-state behavior of HNO3, U, Pu and Tc in TcS. The reliability of the program was verified by cascade extraction experiment, the relative error between calculation value and experiment value is 10% more or less except few spots. The comparison between experiment and calculation results is illustrated in Fig. 1. The technical parameters of TcS were analyzed by this program, it is found that the Decontamination factor (DFTc/U) in TcS is remarkably affected by the overall consumption (multiply molarity by volume flux) of HNO3, DFTc/U is

  16. Structural and bio-technical analysis of terrestrial trout farms in Tokat province

    Directory of Open Access Journals (Sweden)

    Nihat YEŞİLAYAR

    2013-06-01

    Full Text Available This study was carried out by the aim of determining the feasibilities, structural and bio-technical problems of aquaculture facilities in Tokatprovince.Total 7 licensed fish farms and located in the fresh water were determined. All of them were visited and interviewed with their owners face to face. The data were collected in order to determine present condition of fish farms and their problems.Although total project capacities of farms were 96 tons/year, the actual production was found 96 tons/year. Average fishproductionof facilities16tons/yearwas found. The average feed conversion rate (FCR as found 1.09. We have figured out that ponds have not been productively used and stock density is very high in Tokat, present aquaculture investments must rearranged considering scientific data

  17. Zinc selenide photoelectrodes. Efficient radiative recombination in a stable photoelectrochemical cell. Technical report

    Energy Technology Data Exchange (ETDEWEB)

    Smiley, P.M.; Biagioni, R.N.; Ellis, A.B.

    1984-05-25

    Photoluminescence (PL) and electroluminescence (EL) from single-crystal, n-type, A1-doped ZnSe (ZnSe:A1) electrodes have been studied. These samples exhibit both edge emission (lambda(max) approx. 460 nm) and subband gap emission when excited at several ultraband gap wavelengths. The latter PL band is particularly intense, with a measured radiative quantum yield of approx. 0.1 to 0.01; the transition seems at least partially self-activated (SA) in origin, based on previously reported PL data. Excited-state communication involving the two emissive states is inferred from time-resolved PL measurements. Stable photoelectrochemical cells (PEC's) can be constructed from n-ZnSe:A1 electrodes and aqueous diselenide or ditelluride electrolytes. Applied potential quenches both of the photoanodes' PL bands roughly in parallel. The extent of PL quenching is consistent with a dead-layer model previously used to describe quenching in Au-ZnSe Schottky diodes. When used as a dark cathode in aqueous, alkaline peroxydisulfate eletrolyte, EL from ZnSe:A1 electrodes is observed.

  18. Evaluating the effects of scaling up on the performance of bioelectrochemical systems using a technical scale microbial electrolysis cell.

    Science.gov (United States)

    Brown, Robert Keith; Harnisch, Falk; Wirth, Sebastian; Wahlandt, Helge; Dockhorn, Thomas; Dichtl, Norbert; Schröder, Uwe

    2014-07-01

    This study focuses on the challenges of the scaling up process of bioelectrochemical systems on the example of a technical scale microbial electrolysis cell referred to as the "prototype". Anodically treating real wastewater and operated in continuous mode at a hydraulic retention time of 1.23 d with an average chemical oxygen demand (COD)-loading rate of 0.5 g O2 d(-1) L Reactor(-1) the prototype on average showed COD removal efficiency of 67% with effluent concentrations of 210 mg O2 L(-1) and an ammonium elimination rate of 17.8 ± 3.9 mg Nd(-1) L Reactor(-1) resulting in effluent concentrations of 30.7 ± 3.7 mg NL(-1) with a removal efficiency of 40% at a current generation of 72 μA cm(-2) and Coulomb efficiency of 11%. A model is described as a method for comparing conventional and BES based technology using the above mentioned criteria and balancing them against the respective loading rates.

  19. Amorphous thin films for solar cell application. Final technical report, March 15, 1979-February 29, 1980

    Energy Technology Data Exchange (ETDEWEB)

    Jonath, A D; Anderson, W W; Crowley, J L; MacMillan, H F; Junga, F A; Knudsen, J F; Monahan, K M; Thornton, J A

    1980-03-01

    Magnetron sputtering, a deposition method in which magnetic confinement of a plasma encourages high deposition rates at low working gas partial pressures, is under investigation in this program as a candidate production technology for large-scale manufacture of high-efficiency, thin-film amorphous silicon solar photovoltaic cells. The approach uses two dc magnetron geometries: (1) a low-cost planar magnetron (PM) system for exploratory and detailed examination of deposition parameter space; and (2) a cylindrical magnetron (CM) system, scalable to production sizes, for deposition of homogeneous films over large areas. Detailed descriptions of these two systems are included. During this first-year effort, amorphous silicon films and device structures were sputtered in both PM and CM systems under a wide range of deposition conditions (i.e., T/sub s/, P/sub Ar/, P/sub H/sub 2//) using both doped and undoped sputter targets. Measured electrical and optical film properties indicate that control over a wide range of conductivity, photoconductivity, conductivity activation energy, and optical and infrared absorption behavior is achievable. Multiple depositions to fabricate simple MIS device structures and simultaneously to deposit monitor samples of individual constituent layers have been successful. Other program highlights are: (1) deposition rates as great as 1500 A/min were achieved in high-power dc magnetron operation at practical substrate-target spacings; (2) p-type and n-type a-Si:H consistently deposited from p- and n-type targets, respectively; (3) demonstrated correlation of argon and hydrogen partial pressure variations with optical, electronic, and structural properties of magnetron-sputtered a-Si:H films; and (4) initial depositions have achieved properties comparable to those in films made by rf sputtering and glow-discharge methods.

  20. Proteomic Analysis of Chinese Hamster Ovary Cells

    Science.gov (United States)

    Baycin-Hizal, Deniz; Tabb, David L.; Chaerkady, Raghothama; Chen, Lily; Lewis, Nathan E.; Nagarajan, Harish; Sarkaria, Vishaldeep; Kumar, Amit; Wolozny, Daniel; Colao, Joe; Jacobson, Elena; Tian, Yuan; O'Meally, Robert N.; Krag, Sharon S.; Cole, Robert N.; Palsson, Bernhard O.; Zhang, Hui; Betenbaugh, Michael

    2013-01-01

    In order to complement the recent genomic sequencing of Chinese hamster ovary (CHO) cells, proteomic analysis was performed on CHO including the cellular proteome, secretome, and glycoproteome using tandem mass spectrometry (MS/MS) of multiple fractions obtained from gel electrophoresis, multi-dimensional liquid chromatography, and solid phase extraction of glycopeptides (SPEG). From the 120 different mass spectrometry analyses generating 682,097 MS/MS spectra, 93,548 unique peptide sequences were identified with at most a 0.02 false discovery rate (FDR). A total of 6164 grouped proteins were identified from both glycoproteome and proteome analysis, representing an 8-fold increase in the number of proteins currently identified in the CHO proteome. Furthermore, this is the first proteomic study done using CHO genome exclusively which provides for more accurate identification of proteins. From this analysis, the CHO codon frequency was determined and found to be distinct from humans, which will facilitate expression of human proteins in CHO cells. Analysis of the combined proteomic and mRNA data sets indicated the enrichment of a number of pathways including protein processing and apoptosis but depletion of proteins involved in steroid hormone and glycosphingolipid metabolism. 504 of the detected proteins included N-acetylation modifications and 1292 different proteins were observed to be N-glycosylated. This first large-scale proteomic analysis will enhance the knowledge base about CHO capabilities for recombinant expression and provide information useful in cell engineering efforts aimed at modifying CHO cellular functions. PMID:22971049

  1. Public and Private Hospital Services Reform Using Data Envelopment Analysis to Measure Technical, Scale, Allocative, and Cost Efficiencies

    Directory of Open Access Journals (Sweden)

    Ali Emrouznejad

    2012-07-01

    Full Text Available Background: The aim of this study was to suggest a suitable context to develop efficient hospitalsystems while maintaining the quality of care at minimum expenditures.Methods: This research aimed to present a model of efficiency for selected public and privatehospitals of East Azerbaijani Province of Iran by making use of Data Envelopment Analysis approachin order to recognize and suggest the best practice standards.Results: Among the six inefficient hospitals, 2 (33% had a technical efficiency score of lessthan 50% (both private, 2 (33% between 51 and 74% (one private and one public and the rest(2, 33% between 75 and 99% (one private and one public.Conclusion: In general, the public hospitals are relatively more efficient than private ones; it isrecommended for inefficient hospitals to make use of the followings: transferring, selling, orrenting idle/unused beds; transferring excess doctors and nurses to the efficient hospitals orother health centers; pensioning off, early retirement clinic officers, technicians/technologists,and other technical staff. The saving obtained from the above approaches could be used to improveremuneration for remaining staff and quality of health care services of hospitals, rural andurban health centers, support communities to start or sustain systematic risk and resource poolingand cost sharing mechanisms for protecting beneficiaries against unexpected health carecosts, compensate the capital depreciation, increasing investments, and improve diseases preventionservices and facilities in the provincial level.

  2. Measuring and Benchmarking Technical Efficiency of Public Hospitals in Tianjin, China: A Bootstrap-Data Envelopment Analysis Approach.

    Science.gov (United States)

    Li, Hao; Dong, Siping

    2015-01-01

    China has long been stuck in applying traditional data envelopment analysis (DEA) models to measure technical efficiency of public hospitals without bias correction of efficiency scores. In this article, we have introduced the Bootstrap-DEA approach from the international literature to analyze the technical efficiency of public hospitals in Tianjin (China) and tried to improve the application of this method for benchmarking and inter-organizational learning. It is found that the bias corrected efficiency scores of Bootstrap-DEA differ significantly from those of the traditional Banker, Charnes, and Cooper (BCC) model, which means that Chinese researchers need to update their DEA models for more scientific calculation of hospital efficiency scores. Our research has helped shorten the gap between China and the international world in relative efficiency measurement and improvement of hospitals. It is suggested that Bootstrap-DEA be widely applied into afterward research to measure relative efficiency and productivity of Chinese hospitals so as to better serve for efficiency improvement and related decision making.

  3. Technical Analysis of IEC 61850 Ed 2.0%IEC 61850 Ed 2.0技术分析

    Institute of Scientific and Technical Information of China (English)

    任雁铭; 操丰梅; 张军

    2013-01-01

    对IEC 61850 Ed 2.0的文件构成和技术路线进行了介绍.对比IEC 61850 Ed 1.0,从数据模型、工程配置语言和一致性测试3个方面,对IEC 61850 Ed 2.0的技术特点进行了技术分析.从设备制造商和检测中心2个方面,讨论了如何从IEC 61850 Ed 1.0迁徙到IEC 61850 Ed 2.0.%The profile and technical philosophy of IEC 61850 Ed 2.0 are described. By comparison with IEC 61850 Ed 1.0, an analysis is made of the technical features of IEC 61850 Ed 2.0 data model, substation configuration language and conformance test. A discussion is made of shifting from IEC 61850 Ed 1.0 to IEC 61850 Ed 2.0 with respect to the manufacturer and testing organization.

  4. Technical Agency in Practice

    DEFF Research Database (Denmark)

    Krummheuer, Antonia Lina

    2015-01-01

    The paper combines the discussion of technical agency and hybrid networks of Actor-Network Theory (ANT) with an ethnomethodological/conversation analytical (EMCA) perspective on situated practices in which participants ascribe agency to technical artefacts. While ANT works with (ethnographic......) description of hybrid networks in which human and non-human actants are granted agency without differentiating different kinds of agency, EMCA focuses on the member's perspectives and the situated construction of technical agency that is made relevant within an ongoing interaction. Based on an EMCA analysis...... of three video recordings of situations in which technical agency is made relevant by the human participants, the paper demonstrates different ways in which agency is granted to technical artefacts. Human participants can treat a technology as communication partner, as an active part (and actant...

  5. Multifunctional Prenylated Peptides for Live Cell Analysis

    Science.gov (United States)

    Wollack, James W.; Zeliadt, Nicholette A.; Mullen, Daniel G.; Amundson, Gregg; Geier, Suzanne; Falkum, Stacy; Wattenberg, Elizabeth V.; Barany, George; Distefano, Mark D.

    2009-01-01

    Protein prenylation is a common post-translational modification present in eukaryotic cells. Many key proteins involved in signal transduction pathways are prenylated and inhibition of prenylation can be useful as a therapeutic intervention. While significant progress has been made in understanding protein prenylation in vitro, we have been interested in studying this process in living cells, including the question of where prenylated molecules localize. Here, we describe the synthesis and live cell analysis of a series of fluorescently labeled multifunctional peptides, based on the C-terminus of the naturally prenylated protein CDC42. A synthetic route was developed that features a key Acm to Scm protecting group conversion. This strategy was compatible with acid-sensitive isoprenoid moieties, and allowed incorporation of an appropriate fluorophore as well as a cell-penetrating sequence (penetratin). These peptides are able to enter cells through different mechanisms, depending on the presence or absence of the penetratin vehicle and the nature of the prenyl group attached. Interestingly, prenylated peptides lacking penetratin are able to enter cells freely through an energy-independent process, and localize in a perinuclear fashion. This effect extends to a prenylated peptide that includes a full “CAAX box” sequence (specifically, CVLL). Hence, these peptides open the door for studies of protein prenylation in living cells, including enzymatic processing and intracellular peptide trafficking. Moreover, the synthetic strategy developed here should be useful for the assembly of other types of peptides that contain acid sensitive functionalities. PMID:19425596

  6. Technical Letter Report: Evaluation and Analysis of a Few International Periodic Safety Review Summary Reports

    Energy Technology Data Exchange (ETDEWEB)

    Chopra, Omesh K. [Argonne National Lab., IL (United States). Environmental Science Division; Diercks, Dwight R. [Argonne National Lab., IL (United States). Nuclear Engineering Division; Ma, David Chia-Chiun [Argonne National Lab., IL (United States). Environmental Science Division; Garud, Yogendra S. [Argonne National Lab., IL (United States). Environmental Science Division

    2013-12-17

    and 1988. All of the reports reviewed were the regulator’s assessment of the PSR findings rather than the original PSR report, and all but one were English translations from the original language. In these reviews, it was found that most of the countries base their regulatory guidance to some extent (and often to a large extent) on U.S. design codes and standards, NRC regulatory guidance, and U.S. industry guidance. In addition, many of the observed operational technical issues and OpE events reported for U.S. reactors are also cited in the PSR reports. The PSR reports also identified a number of potential technical material/component performance issues and OpE events that are not commonly reported for U.S. plants.

  7. CT-guided vertebroplasty: analysis of technical results, extraosseous cement leakages, and complications in 500 procedures

    Energy Technology Data Exchange (ETDEWEB)

    Pitton, Michael Bernhard; Herber, Sascha; Koch, Ulrike; Oberholzer, Katja; Dueber, Christoph [Johannes Gutenberg-University of Mainz, Department of Diagnostic and Interventional Radiology, Mainz (Germany); Drees, Philip [University Hospital, Johannes Gutenberg-University of Mainz, Department of Orthopedic Surgery, Mainz (Germany)

    2008-11-15

    The aim of this study was to analyze the technical results, the extraosseous cement leakages, and the complications in our first 500 vertebroplasty procedures. Patients with osteoporotic vertebral compression fractures or osteolytic lesions caused by malignant tumors were treated with CT-guided vertebroplasty. The technical results were documented with CT, and the extraosseous cement leakages and periinterventional clinical complications were analyzed as well as secondary fractures during follow-up. Since 2002, 500 vertebroplasty procedures have been performed on 251 patients (82 male, 169 female, age 71.5 {+-} 9.8 years) suffering from osteoporotic compression fractures (n = 217) and/or malignant tumour infiltration (n = 34). The number of vertebrae treated per patient was 1.96 {+-} 1.29 (range 1-10); the numbers of interventions per patient and interventions per vertebra were 1.33 {+-} 0.75 (range 1-6) and 1.01 {+-} 0.10, respectively. The amount of PMMA cement was 4.5 {+-} 1.9 ml and decreased during the 5-year period of investigation. The procedure-related 30-day mortality was 0.4% (1 of 251 patients) due to pulmonary embolism in this case. The procedure-related morbidity was 2.8% (7/251), including one acute coronary syndrome beginning 12 h after the procedure and one missing patellar reflex in a patients with a cement leak near the neuroformen because of osteolytic destruction of the respective pedicle. Additionally, one patient developed a medullary conus syndrome after a fall during the night after vertebroplasty, two patients reached an inadequate depth of conscious sedation, and two cases had additional fractures (one pedicle fracture, one rib fracture). The overall CT-based cement leak rate was 55.4% and included leakages predominantly into intervertebral disc spaces (25.2%), epidural vein plexus (16.0%), through the posterior wall (2.6%), into the neuroforamen (1.6%), into paravertebral vessels (7.2%), and combinations of these and others. During follow

  8. Spent fuel sabotage test program, characterization of aerosol dispersal : technical review and analysis supplement.

    Energy Technology Data Exchange (ETDEWEB)

    Durbin, Samuel G.; Lindgren, Eric Richard

    2009-07-01

    This project seeks to provide vital data required to assess the consequences of a terrorist attack on a spent fuel transportation cask. One such attack scenario involves the use of conical shaped charges (CSC), which are capable of damaging a spent fuel transportation cask. In the event of such an attack, the amount of radioactivity that may be released as respirable aerosols is not known with great certainty. Research to date has focused on measuring the aerosol release from single short surrogate fuel rodlets subjected to attack by a small CSC device in various aerosol chamber designs. The last series of three experiments tested surrogate fuel rodlets made with depleted uranium oxide ceramic pellets in a specially designed double chamber aerosol containment apparatus. This robust testing apparatus was designed to prevent any radioactive release and allow high level radioactive waste disposal of the entire apparatus following testing of actual spent fuel rodlets as proposed. DOE and Sandia reviews of the project to date identified a number of issues. The purpose of this supplemental report is to address and document the DOE review comments and to resolve the issues identified in the Sandia technical review.

  9. Technical and economic analysis of energy efficiency of Chinese room air conditioners

    Energy Technology Data Exchange (ETDEWEB)

    Fridley, David G.; Rosenquist, Gregory; Jiang, Lin; Li, Aixian; Xin, Dingguo; Cheng, Jianhong

    2001-02-01

    China has experienced tremendous growth in the production and sales of room air conditioners over the last decade. Although minimum room air conditioner energy efficiency standards have been in effect since 1989, no efforts were made during most of the 1990's to update the standard to be more reflective of current market conditions. In 1999, China's State Bureau of Technical Supervision (SBTS) included in their annual plan the development and revision of the 1989 room air conditioner standard, and experts from SBTS worked together with LBNL to analyze the new standards. Based on the engineering and life cycle-cost analyses performed, the most predominant type of room air conditioner in the Chinese market (split-type with a cooling capacity between 2500 and 4500 W (8500 Btu/h and 15,300Btu/h)) can have its efficiency increased cost-effectively to an energy efficiency ratio (EER) of 2.92 W/W (9.9 Btu/hr/W). If an EER standard of 2.92 W/W became effective in 2001, Chinese consumers would be estimated to save over 3.5 billion Yuan (420 million U.S. dollars) over the period of 2001-2020. Carbon emissions over the same period would be reduced by approximately 12 million metric tonnes.

  10. Online Monitoring Technical Basis and Analysis Framework for Emergency Diesel Generators - Interim Report for FY 2013

    Energy Technology Data Exchange (ETDEWEB)

    Binh T. Pham; Nancy J. Lybeck; Vivek Agarwal

    2012-12-01

    The Light Water Reactor Sustainability program at Idaho National Laboratory is actively conducting research to develop and demonstrate online monitoring capabilities for active components in existing nuclear power plants. Idaho National Laboratory and the Electric Power Research Institute are working jointly to implement a pilot project to apply these capabilities to emergency diesel generators and generator step-up transformers. The Electric Power Research Institute Fleet-Wide Prognostic and Health Management Software Suite will be used to implement monitoring in conjunction with utility partners: Braidwood Generating Station (owned by Exelon Corporation) for emergency diesel generators, and Shearon Harris Nuclear Generating Station (owned by Duke Energy Progress) for generator step-up transformers. This report presents monitoring techniques, fault signatures, and diagnostic and prognostic models for emergency diesel generators. Emergency diesel generators provide backup power to the nuclear power plant, allowing operation of essential equipment such as pumps in the emergency core coolant system during catastrophic events, including loss of offsite power. Technical experts from Braidwood are assisting Idaho National Laboratory and Electric Power Research Institute in identifying critical faults and defining fault signatures associated with each fault. The resulting diagnostic models will be implemented in the Fleet-Wide Prognostic and Health Management Software Suite and tested using data from Braidwood. Parallel research on generator step-up transformers was summarized in an interim report during the fourth quarter of fiscal year 2012.

  11. Technical and economic analysis of semimechanized harvest of eucalyptus in different spacing

    Directory of Open Access Journals (Sweden)

    Elton da Silva Leite

    2014-12-01

    Full Text Available The objective of this study was to evaluate technically semi-mechanized forest cutting costs in eucalypt plantations in first rotation, average slope of 26.22º (58.2%, spacing 3 x 2.5 m, 3 x 3.33 m and 3 x 4 m, with volumes of 0,326, 0,361 and 0,370 m3 per tree, in the 1+1 system (one chainsaw operator and a helper. The results showed that the activities that consume more time in cutting and processing with the chainsaw Stihl MS360 are: displacement, bucking and 1st, 2nd and 3rd cut. Spaced at 3 x 2.5 m, average productivity of was 4.69 m3·h-1 at a production cost of 5.16 R$·m-3. In the 3 x 3.33 m spacing average productivity was 5.19 m3·h-1 and 4.66 R$·m-3 and in the 3 x 4 m spacing was 5.73 m3·h-1 and 4.22 R$·m-3. The item of greatest influence on operating costs was labor, with 75% of total costs. The wider spacing resulted in higherproductivity and lower production costs.

  12. Assessment of international reference materials for isotope-ratio analysis (IUPAC Technical Report)

    Science.gov (United States)

    Brand, Willi A.; Coplen, Tyler B.; Vogl, Jochen; Rosner, Martin; Prohaska, Thomas

    2014-01-01

    Since the early 1950s, the number of international measurement standards for anchoring stable isotope delta scales has mushroomed from 3 to more than 30, expanding to more than 25 chemical elements. With the development of new instrumentation, along with new and improved measurement procedures for studying naturally occurring isotopic abundance variations in natural and technical samples, the number of internationally distributed, secondary isotopic reference materials with a specified delta value has blossomed in the last six decades to more than 150 materials. More than half of these isotopic reference materials were produced for isotope-delta measurements of seven elements: H, Li, B, C, N, O, and S. The number of isotopic reference materials for other, heavier elements has grown considerably over the last decade. Nevertheless, even primary international measurement standards for isotope-delta measurements are still needed for some elements, including Mg, Fe, Te, Sb, Mo, and Ge. It is recommended that authors publish the delta values of internationally distributed, secondary isotopic reference materials that were used for anchoring their measurement results to the respective primary stable isotope scale.

  13. Online Monitoring Technical Basis and Analysis Framework for Large Power Transformers; Interim Report for FY 2012

    Energy Technology Data Exchange (ETDEWEB)

    Nancy J. Lybeck; Vivek Agarwal; Binh T. Pham; Heather D. Medema; Kirk Fitzgerald

    2012-09-01

    The Light Water Reactor Sustainability program at Idaho National Laboratory (INL) is actively conducting research to develop and demonstrate online monitoring (OLM) capabilities for active components in existing Nuclear Power Plants. A pilot project is currently underway to apply OLM to Generator Step-Up Transformers (GSUs) and Emergency Diesel Generators (EDGs). INL and the Electric Power Research Institute (EPRI) are working jointly to implement the pilot project. The EPRI Fleet-Wide Prognostic and Health Management (FW-PHM) Software Suite will be used to implement monitoring in conjunction with utility partners: the Shearon Harris Nuclear Generating Station (owned by Duke Energy for GSUs, and Braidwood Generating Station (owned by Exelon Corporation) for EDGs. This report presents monitoring techniques, fault signatures, and diagnostic and prognostic models for GSUs. GSUs are main transformers that are directly connected to generators, stepping up the voltage from the generator output voltage to the highest transmission voltages for supplying electricity to the transmission grid. Technical experts from Shearon Harris are assisting INL and EPRI in identifying critical faults and defining fault signatures associated with each fault. The resulting diagnostic models will be implemented in the FW-PHM Software Suite and tested using data from Shearon-Harris. Parallel research on EDGs is being conducted, and will be reported in an interim report during the first quarter of fiscal year 2013.

  14. Using Data Envelopment Analysis to Evaluate Technical Training Program for Maintenance Units

    Directory of Open Access Journals (Sweden)

    Nai-Chieh Wei and Shui-Cheung Cham

    2015-05-01

    Full Text Available Applying data envelope analysis (DEA, this study focuses on the influences of objectivity and subjectivity on aircraft maintenance supervision evaluation and inspection procedure; the aim is to improve overall logistic operation and training efficiency. Moreover, the results of this analysis will be helpful for making decisions in training, management, capability and competitiveness

  15. A technical and economic analysis of one potential pathway to a 100% renewable energy system

    Directory of Open Access Journals (Sweden)

    David Connolly

    2014-02-01

    Full Text Available This paper outlines how an existing energy system can be transformed into a 100% renewable energy system. The transition is divided into a number of key stages which reflect key radical technological changes on the supply side of the energy system. Ireland is used as a case study,but in reality this reflects many typical energy systems today which use power plants for electricity, individual boilers for heat, and oil for transport. The seven stages analysed are 1 reference, 2 introduction of district heating, 3 installation of small and large-scale heat pumps,4 reducing grid regulation requirements, 5 adding flexible electricity demands and electric vehicles, 6 producing synthetic methanol/DME for transport, and finally 7 using synthetic gas to replace the remaining fossil fuels. For each stage, the technical and economic performance of the energy system is calculated. The results indicate that a 100% renewable energy system can provide the same end-user energy demands as today’s energy system and at the same price. Electricity will be the backbone of the energy system, but the flexibility in today’s electricity sector will be transferred from the supply side of the demand side in the future. Similarly, due to changes in the type of spending required in a 100% renewable energy system, this scenario will result in the creation of 100,000 additional jobs in Ireland compared to an energy system like today’s. These results are significant since they indicate that the transition to a 100% renewable energy system can begin today, without increasing the cost of energy in the short- or long-term, if the costs currently forecasted for 2050 become a reality.

  16. Space station system analysis study. Part 3: Documentation. Volume 2: Technical report. [structural design and construction

    Science.gov (United States)

    1977-01-01

    An analysis of construction operation is presented as well as power system sizing requirements. Mission hardware requirements are reviewed in detail. Space construction base and design configurations are also examined.

  17. Time-frequency analysis in optical coherence tomography for technical objects examination

    Science.gov (United States)

    StrÄ kowski, Marcin R.; Kraszewski, Maciej; Trojanowski, Michał; Pluciński, Jerzy

    2014-05-01

    Optical coherence tomography (OCT) is one of the most advanced optical measurement techniques for complex structure visualization. The advantages of OCT have been used for surface and subsurface defect detection in composite materials, polymers, ceramics, non-metallic protective coatings, and many more. Our research activity has been focused on timefrequency spectroscopic analysis in OCT. It is based on time resolved spectral analysis of the backscattered optical signal delivered by the OCT. The time-frequency method gives spectral characteristic of optical radiation backscattered or backreflected from the particular points inside the tested device. This provides more information about the sample, which are useful for further analysis. Nowadays, the applications of spectroscopic analysis for composite layers characterization or tissue recognition have been reported. During our studies we have found new applications of spectroscopic analysis. We have used this method for thickness estimation of thin films, which are under the resolution of OCT. Also, we have combined the spectroscopic analysis with polarization sensitive OCT (PS-OCT). This approach enables to obtain a multiorder retardation value directly and may become a breakthrough in PS-OCT measurements of highly birefringent media. In this work, we present the time-frequency spectroscopic algorithms and their applications for OCT. Also, the theoretical simulations and measurement validation of this method are shown.

  18. Fuel cell hybrid taxi life cycle analysis

    Energy Technology Data Exchange (ETDEWEB)

    Baptista, Patricia, E-mail: patricia.baptista@ist.utl.pt [IDMEC-Instituto Superior Tecnico, Universidade Tecnica de Lisboa, Av. Rovisco Pais, 1, 1049-001 Lisboa (Portugal); Ribau, Joao; Bravo, Joao; Silva, Carla [IDMEC-Instituto Superior Tecnico, Universidade Tecnica de Lisboa, Av. Rovisco Pais, 1, 1049-001 Lisboa (Portugal); Adcock, Paul; Kells, Ashley [Intelligent Energy, Charnwood Building, HolywellPark, Ashby Road, Loughborough, LE11 3GR (United Kingdom)

    2011-09-15

    A small fleet of classic London Taxis (Black cabs) equipped with hydrogen fuel cell power systems is being prepared for demonstration during the 2012 London Olympics. This paper presents a Life Cycle Analysis for these vehicles in terms of energy consumption and CO{sub 2} emissions, focusing on the impacts of alternative vehicle technologies for the Taxi, combining the fuel life cycle (Tank-to-Wheel and Well-to-Tank) and vehicle materials Cradle-to-Grave. An internal combustion engine diesel taxi was used as the reference vehicle for the currently available technology. This is compared to battery and fuel cell vehicle configurations. Accordingly, the following energy pathways are compared: diesel, electricity and hydrogen (derived from natural gas steam reforming). Full Life Cycle Analysis, using the PCO-CENEX drive cycle, (derived from actual London Taxi drive cycles) shows that the fuel cell powered vehicle configurations have lower energy consumption (4.34 MJ/km) and CO{sub 2} emissions (235 g/km) than both the ICE Diesel (9.54 MJ/km and 738 g/km) and the battery electric vehicle (5.81 MJ/km and 269 g/km). - Highlights: > A Life Cycle Analysis of alternative vehicle technologies for the London Taxi was performed. > The hydrogen powered vehicles have the lowest energy consumption and CO{sub 2} emissions results. > A hydrogen powered solution can be a sustainable alternative in a full life cycle framework.

  19. Analysis of the Madaras Rotor Power Plant: an alternate method for extracting large amounts of power from the wind. Volume 2. Technical report

    Energy Technology Data Exchange (ETDEWEB)

    Whitford, D.H.; Minardi, J.E.; West, B.S.; Dominic, R.J.

    1978-06-01

    The purpose of the program was to analyze and up-date the design of the Madaras Rotor Power Plant concept that had been developed in the 1930's to determine the technical and economic feasibility of this system to be competitive with conventional horizontal axis wind turbines. A four-task program consisting of a series of wind tunnel tests, an electro-mechanical analysis, a performance analysis, and a cost analysis was conducted.

  20. Texas Hydrogen Highway Fuel Cell Hybrid Bus and Fueling Infrastructure Technology Showcase - Final Scientific/Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Hitchcock, David

    2012-06-29

    The Texas Hydrogen Highway project has showcased a hydrogen fuel cell transit bus and hydrogen fueling infrastructure that was designed and built through previous support from various public and private sector entities. The aim of this project has been to increase awareness among transit agencies and other public entities on these transportation technologies, and to place such technologies into commercial applications, such as a public transit agency. The initial project concept developed in 2004 was to show that a skid-mounted, fully-integrated, factory-built and tested hydrogen fueling station could be used to simplify the design, and lower the cost of fueling infrastructure for fuel cell vehicles. The approach was to design, engineer, build, and test the integrated fueling station at the factory then install it at a site that offered educational and technical resources and provide an opportunity to showcase both the fueling station and advanced hydrogen vehicles. The two primary technology components include: Hydrogen Fueling Station: The hydrogen fueling infrastructure was designed and built by Gas Technology Institute primarily through a funding grant from the Texas Commission on Environmental Quality. It includes hydrogen production, clean-up, compression, storage, and dispensing. The station consists of a steam methane reformer, gas clean-up system, gas compressor and 48 kilograms of hydrogen storage capacity for dispensing at 5000 psig. The station is skid-mounted for easy installation and can be relocated if needed. It includes a dispenser that is designed to provide temperaturecompensated fills using a control algorithm. The total station daily capacity is approximately 50 kilograms. Fuel Cell Bus: The transit passenger bus built by Ebus, a company located in Downey, CA, was commissioned and acquired by GTI prior to this project. It is a fuel cell plug-in hybrid electric vehicle which is ADA compliant, has air conditioning sufficient for Texas operations

  1. Technical Network

    CERN Multimedia

    2007-01-01

    In order to optimize the management of the Technical Network (TN), to ease the understanding and purpose of devices connected to the TN, and to improve security incident handling, the Technical Network Administrators and the CNIC WG have asked IT/CS to verify the "description" and "tag" fields of devices connected to the TN. Therefore, persons responsible for systems connected to the TN will receive email notifications from IT/CS asking them to add the corresponding information in the network database. Thank you very much for your cooperation. The Technical Network Administrators & the CNIC WG

  2. Technical Network

    CERN Multimedia

    2007-01-01

    In order to optimise the management of the Technical Network (TN), to facilitate understanding of the purpose of devices connected to the TN and to improve security incident handling, the Technical Network Administrators and the CNIC WG have asked IT/CS to verify the "description" and "tag" fields of devices connected to the TN. Therefore, persons responsible for systems connected to the TN will receive e-mails from IT/CS asking them to add the corresponding information in the network database at "network-cern-ch". Thank you very much for your cooperation. The Technical Network Administrators & the CNIC WG

  3. The Naval Enlisted Professional Development Information System (NEPDIS): Front End Analysis (FEA) Process. Technical Report 159.

    Science.gov (United States)

    Aagard, James A.; Ansbro, Thomas M.

    The Naval Enlisted Professional Development Information System (NEPDIS) was designed to function as a fully computerized information assembly and analysis system to support labor force, personnel, and training management. The NEPDIS comprises separate training development, instructional, training record and evaluation, career development, and…

  4. Fluid Centrality: A Social Network Analysis of Social-Technical Relations in Computer-Mediated Communication

    Science.gov (United States)

    Enriquez, Judith Guevarra

    2010-01-01

    In this article, centrality is explored as a measure of computer-mediated communication (CMC) in networked learning. Centrality measure is quite common in performing social network analysis (SNA) and in analysing social cohesion, strength of ties and influence in CMC, and computer-supported collaborative learning research. It argues that measuring…

  5. Hot Cell Facility (HCF) Safety Analysis Report

    Energy Technology Data Exchange (ETDEWEB)

    MITCHELL,GERRY W.; LONGLEY,SUSAN W.; PHILBIN,JEFFREY S.; MAHN,JEFFREY A.; BERRY,DONALD T.; SCHWERS,NORMAN F.; VANDERBEEK,THOMAS E.; NAEGELI,ROBERT E.

    2000-11-01

    This Safety Analysis Report (SAR) is prepared in compliance with the requirements of DOE Order 5480.23, Nuclear Safety Analysis Reports, and has been written to the format and content guide of DOE-STD-3009-94 Preparation Guide for U. S. Department of Energy Nonreactor Nuclear Safety Analysis Reports. The Hot Cell Facility is a Hazard Category 2 nonreactor nuclear facility, and is operated by Sandia National Laboratories for the Department of Energy. This SAR provides a description of the HCF and its operations, an assessment of the hazards and potential accidents which may occur in the facility. The potential consequences and likelihood of these accidents are analyzed and described. Using the process and criteria described in DOE-STD-3009-94, safety-related structures, systems and components are identified, and the important safety functions of each SSC are described. Additionally, information which describes the safety management programs at SNL are described in ancillary chapters of the SAR.

  6. Low-density subculture: a technical note on the importance of avoiding cell-to-cell contact during mesenchymal stromal cell expansion.

    Science.gov (United States)

    Balint, Richard; Richardson, Stephen M; Cartmell, Sarah H

    2015-10-01

    Numerous scientific studies and clinical trials are carried out each year exploring the use of mesenchymal stromal cells in regenerative medicine and tissue engineering. However, the effective and reliable expansion of this very important cell type remains a challenge. In this study the importance of cell-to-cell contact during expansion has been explored on the proliferation and differentiation potential of the produced cells. Cells were cultured up to passage 5 under conditions where cell-to-cell contact was either probable (40-70% confluence; see supporting information, Protocol A) or where it was unlikely (10-50% confluence; see supporting information, Protocol B). The effect of the two different conditions on expansion efficiency; proliferation rate and tri-lineage differentiation potential was assessed. Differences in immunophenotype, cell size and senescence were also investigated. Protocol B cultures expanded twice as fast as those cultured with Protocol A. In passage 5 experiments low confluence expanded cells displayed a 10% higher overall proliferation rate, and produced 23% more cells in growth, 12% more in osteogenic, 77% more in adipogenic, but 27% less in chondrogenic medium. Differentiation potential wasn't decisively affected at the mRNA level. However, Protocol B favoured bone and cartilage differentiation at the secretional level. Protocol A populations showed reduced purity, expressing CD105 in only 76% compared to the 96.7% in Protocol B cultures. Protocol A populations also contained significantly more (+4.2%) senescent cells, however, no difference was found in cell size between the two protocols. The findings of this study suggest that cell-to-cell contact, and therefore high confluence levels, is detrimental to MSC quality.

  7. Energy Assurance Technical Training and Awareness Program/Energy Infrastructure Training and Analysis Center

    Energy Technology Data Exchange (ETDEWEB)

    Barbara McCabe

    2005-11-15

    This report covers the work completed during Year One (Year One has a 16 month project period) of a five- year Cooperative Agreement (DE-FC26-03NT41895) between the International Union of Operating Engineers (IUOE) National Hazmat Program (OENHP) and the U. S. Department of Energy (DOE) National Energy Technology Laboratory (NETL). This final technical report is being submitted, as required by the Cooperative Agreement, within 90 (calendar) days after the project period ends (December 31, 2004). The resources allocated to Year One of the Cooperative Agreement were adequate for the completion of the required deliverables. All deliverables have been completed and sent to AAD Document Control as directed in the cooperative agreement. The allocation for Year One required 20-25 trainers to be trained in each of five Train-the-Trainer courses and a total of 6,000 workers trained throughout the country. Through cost savings employed for the scheduling and conduct of Train-the-Trainer, instructor refreshers, and direct training classes, 3171 workers have been trained to date. This total incorporates 159 trainers and members from management, local, county, state and federal organizations identified in the Strategic Plan. The largest percentage of personnel trained is heavy equipment operators, and building engineers, which is the largest targeted population identified under this cooperative agreement. The OENHP, using existing curriculum as appropriate, has modified and developed new training modules that have been used to establish four different levels of training courses. The four courses are: (1) EA 500 Energy Assurance Train-the-Trainer, (2) EA 400 Energy Assurance Instructor Refresher, (3) EA 300 Energy Assurance, and (4) EA 100 Energy Assurance Awareness. Training modules cover topics, such as, but not limited to, facility vulnerability and vulnerability assessment, physical security- heating, ventilation, air conditioning, terrorism awareness, weapons of mass

  8. 49 CFR 552.6 - Technical review.

    Science.gov (United States)

    2010-10-01

    ... 49 Transportation 6 2010-10-01 2010-10-01 false Technical review. 552.6 Section 552.6... § 552.6 Technical review. The appropriate Associate Administrator conducts a technical review of the petition. The technical review may consist of an analysis of the material submitted, together...

  9. Development of students' conceptual thinking by means of video analysis and interactive simulations at technical universities

    Science.gov (United States)

    Hockicko, Peter; Krišt‧ák, L.‧uboš; Němec, Miroslav

    2015-03-01

    Video analysis, using the program Tracker (Open Source Physics), in the educational process introduces a new creative method of teaching physics and makes natural sciences more interesting for students. This way of exploring the laws of nature can amaze students because this illustrative and interactive educational software inspires them to think creatively, improves their performance and helps them in studying physics. This paper deals with increasing the key competencies in engineering by analysing real-life situation videos - physical problems - by means of video analysis and the modelling tools using the program Tracker and simulations of physical phenomena from The Physics Education Technology (PhET™) Project (VAS method of problem tasks). The statistical testing using the t-test confirmed the significance of the differences in the knowledge of the experimental and control groups, which were the result of interactive method application.

  10. Technical aspects of nuclear microprobe analysis of senile plaques from alzheimer patients

    Science.gov (United States)

    Larsson, N. P.-O.; Tapper, U. A. S.; Sturesson, K.; Odselius, R.; Brun, A.

    1990-04-01

    Alzheimer's disease, a common form of senile dementia, has been proposed to be caused by aluminium. One of the interesting structures to be studied, senile plaque cores in the brain, have centres of only about 10 μm. We have investigated the possibility of applying nuclear microprobes to sections containing senile plaques. An alternative staining procedure, TMToluidin blue staining using a spray technique, is also presented. An outline is given of a procedure for preparing senile plaque specimens for nuclear microprobe analysis. This includes a technique for accurate ion beam positioning, utilizing electron microscopy-grids. The subject may be of general interest since sample preparation is one of the most important aspects in microprobe analysis of biological matter.

  11. Cost-Effectiveness Analysis of the 2009 and 2012 IECC Residential Provisions – Technical Support Document

    Energy Technology Data Exchange (ETDEWEB)

    Mendon, Vrushali V.; Lucas, Robert G.; Goel, Supriya

    2012-12-04

    This analysis was conducted by Pacific Northwest National Laboratory (PNNL) in support of the U.S. Department of Energy’s (DOE) Building Energy Codes Program (BECP). DOE supports the development and adoption of efficient residential and commercial building energy codes. These codes set the minimum requirements for energy efficient building design and construction and ensure energy savings on a national level. This analysis focuses on one and two family dwellings, townhomes, and low-rise multifamily residential buildings. For these buildings, the basis of the energy codes is the International Energy Conservation Code (IECC). This report does not address commercial and high-rise residential buildings, which reference ANSI/ASHRAE/IES Standard 90.1.

  12. Final Technical Report: Advanced Measurement and Analysis of PV Derate Factors.

    Energy Technology Data Exchange (ETDEWEB)

    King, Bruce Hardison [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Burton, Patrick D. [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Hansen, Clifford [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Jones, Christian Birk [Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

    2015-12-01

    The Advanced Measurement and Analysis of PV Derate Factors project focuses on improving the accuracy and reducing the uncertainty of PV performance model predictions by addressing a common element of all PV performance models referred to as “derates”. Widespread use of “rules of thumb”, combined with significant uncertainty regarding appropriate values for these factors contribute to uncertainty in projected energy production.

  13. Final technical report: analysis of molecular data using statistical and evolutionary approaches

    Energy Technology Data Exchange (ETDEWEB)

    Atteson, K.; Junhyong Kim

    2000-02-15

    This document describes the research and training accomplishments of Dr. Kevin Atteson during the DOE fellowship period of September 1997 to September 1999. Dr. Atteson received training in molecular evolution during this period and made progress on seven research topics including: computation of DNA pattern probability, asymptotic redundancy of Bayes rules, performance of neighbor-joining evolutionary tree estimation, convex evolutionary tree estimation, identifiability of trees under mixed rates, gene expression analysis, and population genetics of unequal crossover.

  14. Experimental Modal Analysis and Dynamic Component Synthesis. Volume 1. Summary of Technical Work

    Science.gov (United States)

    1987-12-01

    Rades. M., "Identification of the Dynamic Characteristics of a Simple System with Quadratic Damping," Serie de Mecanique Appliquee. 28 (4), 1983, pp...Measurement and Analysis Requirements," SAE Paper Number 751066 1975, 17 pp. [261 Prony, R. "Essai Experimental et Analytique sur les Lois de [a...of the Dynamic Characteristics of a Simple System with Quadratic Damping, Serie de Mecanique Appliquee, Vol. 28, Num. 4, 1983, pp. 439-446. 11391

  15. Reliability Information Analysis Center 1st Quarter 2007, Technical Area Task (TAT) Report

    Science.gov (United States)

    2007-02-05

    07 planning conference 14 Dec 06 II Marine Expeditionary Force (MEF) meeting with Major Smith 14 Dec 06 Gulf of Mexico Tyndall Air Force Base Missile...Restructured action item spreadsheet " Reviewed the following storyboards (functional flow, graphics and text): 1. 050101 Main Rotor System components 2... storyboards (functional flow, graphics, and text): o 050101 Main Rotor System components. Reliability Information Analysis Center 6000 Flanagan Road

  16. Federal Assistance Program Quarterly Project Progress Report. Geothermal Energy Program: Information Dissemination, Public Outreach, and Technical Analysis Activities. Reporting Period: January 1 - March 31, 2001 [Final report

    Energy Technology Data Exchange (ETDEWEB)

    Lund, John W.

    2002-03-22

    The final report of the accomplishments of the geothermal energy program: information dissemination, public outreach and technical analysis activities by the project team consisting of the Geo-Heat Center, Geothermal Resources Council, Geothermal Education Office, Geothermal Energy Association and the Washington State University Energy Program.

  17. Case-study application of venture analysis: the integrated energy utility. Volume 2. Technical report

    Energy Technology Data Exchange (ETDEWEB)

    Fein, E; Gordon, T J; King, R; Kropp, F G; Shuchman, H L; Stover, J; Hausz, W; Meyer, C

    1978-11-01

    Application of venture analysis would, at a minimum, need to address issues involving careful definition of the product/service being considered; market needs that the product will satisfy; investment/manufacturing costs; minimum selling price needed to achieve desired ROI or other financial measure; market potential at relevant prices; potential for competitors to obsolete the product before investment is recovered; assessment of companies' resources and capabilities to supply the product. There is clearly no single method for performing every venture analysis, because the economic and structural environment associated with each industry varies widely. These and other factors produce differences in cost structure, marketing organizations, and nature of products which dictate that an appropriate method of venture analysis must be tailored to each industry. The initial chapter of the report presents some brief remarks concerning the important concepts that all venture analyses must consider and then describes in detail the method used for the venture analyzed in this report. The case study addresses IEUS for commercialization. The type of IEUS investigated supplies electricity and thermal energy; the thermal energy distributed in the form of high-temperature water, i.e., water from at least 90/sup 0/C upwards to 200/sup 0/C. (MCW)

  18. Systems Analysis Programs for Hands-On Integrated Reliability Evaluations (SAPHIRE) Technical Reference Manual

    Energy Technology Data Exchange (ETDEWEB)

    C. L. Smith; W. J. Galyean; S. T. Beck

    2006-07-01

    The Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE) is a software application developed for performing a complete probabilistic risk assessment (PRA) using a personal computer (PC) running the Microsoft Windows? operating system. Herein information is provided on the principles used in the construction and operation of Version 6.0 and 7.0 of the SAPHIRE system. This report summarizes the fundamental mathematical concepts of sets and logic, fault trees, and probability. This volume then describes the algorithms used to construct a fault tree and to obtain the minimal cut sets. It gives the formulas used to obtain the probability of the top event from the minimal cut sets, and the formulas for probabilities that apply for various assumptions concerning reparability and mission time. It defines the measures of basic event importance that SAPHIRE can calculate. This volume gives an overview of uncertainty analysis using simple Monte Carlo sampling or Latin Hypercube sampling, and states the algorithms used by this program to generate random basic event probabilities from various distributions. Also covered are enhance capabilities such as seismic analysis, cut set "recovery," end state manipulation, and use of "compound events."

  19. Systems Analysis Programs for Hands-On Integrated Reliability Evaluations (SAPHIRE) Technical Reference

    Energy Technology Data Exchange (ETDEWEB)

    C. L. Smith; W. J. Galyean; S. T. Beck

    2008-08-01

    The Systems Analysis Programs for Hands-on Integrated Reliability Evaluations (SAPHIRE) is a software application developed for performing a complete probabilistic risk assessment (PRA) using a personal computer (PC) running the Microsoft Windows? operating system. Herein information is provided on the principles used in the construction and operation of Version 6.0 and 7.0 of the SAPHIRE system. This report summarizes the fundamental mathematical concepts of sets and logic, fault trees, and probability. This volume then describes the algorithms used to construct a fault tree and to obtain the minimal cut sets. It gives the formulas used to obtain the probability of the top event from the minimal cut sets, and the formulas for probabilities that apply for various assumptions concerning reparability and mission time. It defines the measures of basic event importance that SAPHIRE can calculate. This volume gives an overview of uncertainty analysis using simple Monte Carlo sampling or Latin Hypercube sampling, and states the algorithms used by this program to generate random basic event probabilities from various distributions. Also covered are enhance capabilities such as seismic analysis, cut set "recovery," end state manipulation, and use of "compound events."

  20. Local cell metrics: a novel method for analysis of cell-cell interactions

    Directory of Open Access Journals (Sweden)

    Chen Chien-Chiang

    2009-10-01

    Full Text Available Abstract Background The regulation of many cell functions is inherently linked to cell-cell contact interactions. However, effects of contact interactions among adherent cells can be difficult to detect with global summary statistics due to the localized nature and noise inherent to cell-cell interactions. The lack of informatics approaches specific for detecting cell-cell interactions is a limitation in the analysis of large sets of cell image data, including traditional and combinatorial or high-throughput studies. Here we introduce a novel histogram-based data analysis strategy, termed local cell metrics (LCMs, which addresses this shortcoming. Results The new LCM method is demonstrated via a study of contact inhibition of proliferation of MC3T3-E1 osteoblasts. We describe how LCMs can be used to quantify the local environment of cells and how LCMs are decomposed mathematically into metrics specific to each cell type in a culture, e.g., differently-labelled cells in fluorescence imaging. Using this approach, a quantitative, probabilistic description of the contact inhibition effects in MC3T3-E1 cultures has been achieved. We also show how LCMs are related to the naïve Bayes model. Namely, LCMs are Bayes class-conditional probability functions, suggesting their use for data mining and classification. Conclusion LCMs are successful in robust detection of cell contact inhibition in situations where conventional global statistics fail to do so. The noise due to the random features of cell behavior was suppressed significantly as a result of the focus on local distances, providing sensitive detection of cell-cell contact effects. The methodology can be extended to any quantifiable feature that can be obtained from imaging of cell cultures or tissue samples, including optical, fluorescent, and confocal microscopy. This approach may prove useful in interpreting culture and histological data in fields where cell-cell interactions play a critical

  1. Technical Agency in Practice

    DEFF Research Database (Denmark)

    Krummheuer, Antonia Lina

    2015-01-01

    ) description of hybrid networks in which human and non-human actants are granted agency without differentiating different kinds of agency, EMCA focuses on the member's perspectives and the situated construction of technical agency that is made relevant within an ongoing interaction. Based on an EMCA analysis...... of three video recordings of situations in which technical agency is made relevant by the human participants, the paper demonstrates different ways in which agency is granted to technical artefacts. Human participants can treat a technology as communication partner, as an active part (and actant...... in which the human participants orient towards the artefact in different ways; thereby, the construction of technical agency can shift from moment to moment....

  2. Single cell transcriptome analysis using next generation sequencing.

    OpenAIRE

    Blattner, M.

    2010-01-01

    The heterogeneity of tissues, especially in cancer research, is a central issue in transcriptome analysis. In recent years, research has primarily focused on the development of methods for single cell analysis. Single cell analysis aims at gaining (novel) insights into biological processes of healthy and diseased cells. Some of the challenges in transcriptome analysis concern low abundance of sample starting material, necessary sample amplification steps and subsequent analysis. In this study...

  3. Comparative effects of technical toxaphene, 2,5-dichloro-3-biphenylol and octabromodiphenylether on cell viability, [Ca2+]i levels and membrane fluidity in mouse thymocytes.

    Science.gov (United States)

    Sandal, Suleyman; Yilmaz, Bayram; Chen, Chang-Hwei; Carpenter, David O

    2004-08-01

    Flow cytometric studies of mouse thymocytes show that technical toxaphene (10-20 ppm) and 2,5-dichloro-3-biphenylol (PCB 9-OH) (5-10 ppm) kill cells and cause an increase in intracellular calcium concentration, [Ca2+]i, whereas commercial octabromodiphenylether (OBDE) has no effect. The cell death is not a result of the rise of [Ca2+]i, since the divalent cation ionophore, ionomycin, causes a large elevation in [Ca2+]i without cell death. We have studied effects of these compounds on membrane fluorescence polarization, a measure of membrane fluidity, using 1,6-diphenyl-1,3,5-hexatriene (DPH). We find that toxaphene causes a decrease in membrane fluidity in the concentration range associated with cell death, whereas PCB 9-OH causes an increase in fluidity and OBDE has no effect. These observations suggest that alterations of membrane fluidity of thymocytes, whether it be an increase or decrease, can cause cytotoxicity.

  4. Hazards assessment and technical actions due to the production of pressured hydrogen within a pilot photovoltaic-electrolyser-fuel cell power system for agricultural equipment

    Directory of Open Access Journals (Sweden)

    Simone Pascuzzi

    2016-06-01

    Full Text Available A pilot power system formed by photovoltaic panels, alkaline electrolyser and fuel cell stacks was designed and set up to supply the heating system of an experimental greenhouse. The aim of this paper is to analyse the main safety aspects of this power system connected to the management of the pressured hydrogen, such as the explosion limits of the mixture hydrogen-oxygen, the extension of the danger zone, the protection pressure vessels and the system to make unreactive the plant. The electrolyser unit is the core of this plant and from the safety point of view has been equipped with devices able to highlight the malfunctions before they cause damages. Alarm situations are highlighted and the production process is cut off in safe conditions in the event that the operational parameters have an abnormal deviation from the design values. Also the entire power system has been designed so that any failure to its components does not compromise the workers’ safety even if the risk analysis is in progress because technical operations are being carried out for enhancing the plant functionality, making it more suitable to the designed task of supplying electrically the greenhouse heating system during cold periods. Some experimental data pertinent to the solar radiation and the corresponding hydrogen production rate are also reported. At present it does not exist a well-established safety reference protocol to design the reliability of these types of power plants and then the assumed safety measures even if related to the achieved pilot installation, can represent an original base of reference to set up guidelines for designing the safety of power plants in the future available for agricultural purposes.

  5. Technical Note: A simple generalization of the Brutsaert and Nieber analysis

    Directory of Open Access Journals (Sweden)

    T. L. Chor

    2014-11-01

    Full Text Available The Brutsaert and Nieber (1977 analysis is a well known method that can estimate soil parameters given discharge data for some aquifers. It has been used for several cases where the observed late-time behavior of the recession suggests that the water stream that is adjacent to the aquifer has non-zero depth. However, its mathematical formulation is, strictly speaking, not capable of reproducing these real-case scenarios since the early time behavior is based on a solution for which the aquifer stream has zero depth (Polubarinova-Kochina, 1962. We propose a simple generalization for the Brutsaert and Nieber (1977 method that can estimate soil parameters for aquifers discharging into a water stream of finite non-zero depth. The generalization is based on already available solutions by Polubarinova-Kochina (1962, Chor et al. (2013 and Dias et al. (2014 and can be readily implemented with little effort. A sensitivity analysis shows that the modification can have significant impact on the predicted values of the drainable porosity.

  6. Technical analysis for the establishment of the test and design requirement of FTL

    Energy Technology Data Exchange (ETDEWEB)

    Sim, B.S.; Wu, J.S.; Kim, H.R. [and others

    1999-03-01

    A task force team was made up to establish the design and test requirements of the fuel test loop(FTL) which will be installed in HANARO. The activities of the task force team include the review of the performance of the FTL, the establishment of user requirement, the review of interface between HANARO and FTL, the reevaluation of design concept and finally the provision of the viable design option through the impact analysis on design change. Based on the review, the task force team has finalized the design concept of the in-pile test section(IPS) which accommodates a maximum number of 7 fuel pins. Following the modification of the IPS, we have identified the required design modification of the out-file system(OPS) to reduce the investment cost FTL facility while maintaining the performance and not jeopardizing the safety of system. The result of the analysis will be used for the designchange of both IPS and OPS system of FTL and for the future works of the project. (author). 30 tabs., 104 figs.

  7. Analysis of the economic feasibility of the Kloswall longwall mining system. Final technical report: volume 1

    Energy Technology Data Exchange (ETDEWEB)

    Schroeder, E. W.; Davis, R. P.; Gabrill, C. P.; Pimental, R. A.; Douglas, W. J.

    1978-12-01

    This study was performed to evaluate the economic feasibility of the Kloswall longwall mining system. The Kloswall system utilizes a deep web mining concept. It was studied both to determine its own economic efficiency and to compare it to the economic efficiency of conventional longwall and continuous mining systems. A broad-range of mine scenarios and equipment configurations was studied for each mining system. The principal economic factor used in the analysis was Rate of Return on Investment. This factor was derived through a yearly discounted cash flow analysis. An estimated value for yearly Cost per Ton has also been provided. The Kloswall system is shown to be a feasible concept from an economic point-of-view and to provide an economic advantage over conventional longwall systems in seam heights in the range of 6 to 9 feet. This is predicted to be true even if there are relatively large errors in initial cost estimates for the Kloswall equipment. Additionally, by simultaneously considering the nature of equipment cost variations and production rates, the study was able to ascertain optimal longwall mine layouts for the scenarios studied.

  8. Scientometric analysis: A technical need for medical science researchers either as authors or as peer reviewers.

    Science.gov (United States)

    Masic, Izet

    2016-01-01

    The nature of performing a scientific research is a process that has several different components which consist of identifying the key research question(s), choices of scientific approach for the study and data collection, data analysis, and finally reporting on results. Generally, peer review is a series of procedures in the evaluation of a creative work or performance by other people, who work in the same or related field, with the aim of maintaining and improving the quality of work or performance in that field. The assessment of the achievement of every scientist, and thus indirectly determining his reputation in the scientific community of these publications, especially journals, is done through the so-called impact factor index. The impact factor predicts or estimates that how many annual citations article may receive after its publication. Evaluation of scientific productivity and assessment of the published articles of researchers and scientists can be made through the so-called H-index. The quality of published results of scientific work largely depends on knowledge sources that are used in the preparation, which means that it should be considered to serve the purpose and the very relevance of the information used. Scientometrics as a field of science covers all aforementioned issues, and scientometric analysis is obligatory for quality assessment of the scientific validity of published articles and other type of publications.

  9. Total Quality Management (TQM) in Self-Financed Technical Institutions: A Quality Function Deployment (QFD) and Force Field Analysis Approach

    Science.gov (United States)

    Thakkar, Jitesh; Deshmukh, S. G.; Shastree, Anil

    2006-01-01

    Purpose: To explore the potential for adoption of TQM in self-financed technical institutions in the light of new demands and challenges posed by customers/students and society. Design/methodology/approach: The paper presents use of quality function deployment (QFD) which prioritizes technical requirements and correlates them with various…

  10. Single-cell analysis: Advances and future perspectives

    Directory of Open Access Journals (Sweden)

    Emir Hodzic

    2016-11-01

    Full Text Available The last several years have seen rapid development of technologies and methods that permit a detailed analysis of the genome and transcriptome of a single cell. Recent evidence from studies of single cells reveals that each cell type has a distinct lineage and function. The lineage and stage of development of each cell determine how they respond to each other and the environment. Experimental approaches that utilize single-cell analysis are effective means to understand how cell networks work in concert to coordinate a response at the population level; recent progress in single-cell analysis is offering a glimpse at the future.

  11. Visually Augmented Analysis of Socio-Technical Networks in Engineering Systems Design Research

    DEFF Research Database (Denmark)

    Storga, M.; Stankovic, T.; Cash, Philip;

    2013-01-01

    , but only fairly recently has the study of networks in general become a major topic of research in complex engineering systems. The research reported in this paper is discussing how the visually augmented analysis of complex socio-networks (networks of people and technology engaged in a product....../service-system (PSS) life cycle) may be applied in engineering design research. Network thinking of the kind described in this paper could be fundamental for developing new and effective techniques for solving the problems in the engineering design research related to the interpretation of the huge amount of data...... captured during experiments and observations that are more and more used as a main research method. Case studies that are presented illustrate also the significance of the network based research approach in providing insight into ways of improving the design process for complex engineering systems....

  12. Computer Virus Technical Analysis%计算机病毒技术分析

    Institute of Scientific and Technical Information of China (English)

    刘晋辉

    2012-01-01

    对现有的计算机病毒常用技术进行研究和分析,从计算机病毒的种类和特点入手,阐述当前计算机病毒破坏操作系统所采用的主要技术手段及造成的影响,并探讨计算机病毒检测技术的发展趋势.该研究可为计算机病毒检测提供参考.%Research and analysis of existing computer viruses commonly used technology, from the types and characteristics of computer viruses approach to explain the current computer virus destroyed the main operating system used and the impact of technological means, and to explore the computer virus detection technology trends. The study can provide a reference for computer virus detection

  13. [Geothermal system temperature-depth database and model for data analysis]. 5. quarterly technical progress report

    Energy Technology Data Exchange (ETDEWEB)

    Blackwell, D.D.

    1998-04-25

    During this first quarter of the second year of the contract activity has involved several different tasks. The author has continued to work on three tasks most intensively during this quarter: the task of implementing the data base for geothermal system temperature-depth, the maintenance of the WWW site with the heat flow and gradient data base, and finally the development of a modeling capability for analysis of the geothermal system exploration data. The author has completed the task of developing a data base template for geothermal system temperature-depth data that can be used in conjunction with the regional data base that he had already developed and is now implementing it. Progress is described.

  14. Fluid dynamic analysis of a continuous stirred tank reactor for technical optimization of wastewater digestion.

    Science.gov (United States)

    Hurtado, F J; Kaiser, A S; Zamora, B

    2015-03-15

    Continuous stirred tank reactors (CSTR) are widely used in wastewater treatment plants to reduce the organic matter and microorganism present in sludge by anaerobic digestion. The present study carries out a numerical analysis of the fluid dynamic behaviour of a CSTR in order to optimize the process energetically. The characterization of the sludge flow inside the digester tank, the residence time distribution and the active volume of the reactor under different criteria are determined. The effects of design and power of the mixing system on the active volume of the CSTR are analyzed. The numerical model is solved under non-steady conditions by examining the evolution of the flow during the stop and restart of the mixing system. An intermittent regime of the mixing system, which kept the active volume between 94% and 99%, is achieved. The results obtained can lead to the eventual energy optimization of the mixing system of the CSTR.

  15. From data storage towards decision making: LHC technical data integration and analysis

    CERN Document Server

    Marsili, A; Nordt, A; Sapinski, M

    2011-01-01

    The monitoring of the beam conditions, equipment conditions and measurements from the beam instrumentation devices in CERN’s Large Hadron Collider (LHC) produce more than 100 Gb/day of data. Such a big quantity of data is unprecedented in accelerator monitoring and new developments are needed to access, process, combine and analyse data from different equipments. The Beam Loss Monitoring (BLM) system has been one of the most reliable equipments in the LHC during its 2010 run, issuing beam dumps when the detected losses were above the defined abort thresholds. Furthermore, the BLM system was able to detect and study unexpected losses, requiring intensive offline analysis. This article describes the techniques developed to: access the data produced (' 50000 values/s); access relevant system layout information; access, combine and display different machine data.

  16. 2009 Technical Risk and Uncertainty Analysis of the U.S. Department of Energy's Solar Energy Technologies Program Concentrating Solar Power and Photovoltaics R&D

    Energy Technology Data Exchange (ETDEWEB)

    McVeigh, J.; Lausten, M.; Eugeni, E.; Soni, A.

    2010-11-01

    The U.S. Department of Energy (DOE) Solar Energy Technologies Program (SETP) conducted a 2009 Technical Risk and Uncertainty Analysis to better assess its cost goals for concentrating solar power (CSP) and photovoltaic (PV) systems, and to potentially rebalance its R&D portfolio. This report details the methodology, schedule, and results of this technical risk and uncertainty analysis.

  17. 浅析专业技术人员职称评审%Analysis of Professional and Technical Personnel Titles Evaluation

    Institute of Scientific and Technical Information of China (English)

    薛艳洪

    2015-01-01

    Professional and technical personnel is the important technical support and core human resources of enterprise development. Title Appraisal of professional and technical personnel, is conducive to the value of the development of professional and technical personnel, to promote the continuous growth of talent's team of the enterprise. Therefore, we should review the professional and technical personnel objectively, fairly and effectively.%专业技术人员是是企业发展的重要技术支撑和核心人力资源.对专业技术人员进行职称评审,有利于开发专业技术人员价值,促进企业人才队伍不断发展壮大.因此,应该对专业技术人员进行客观、公正、有效的职称评审.

  18. Dichlorodiphenyltrichloroethane technical mixture regulates cell cycle and apoptosis genes through the activation of CAR and ERα in mouse livers

    Energy Technology Data Exchange (ETDEWEB)

    Kazantseva, Yuliya A.; Yarushkin, Andrei A. [Institute of Molecular Biology and Biophysics SB RAMS, Novosibirsk, Timakova str., 2, 630117 (Russian Federation); Pustylnyak, Vladimir O., E-mail: pustylnyak@ngs.ru [Institute of Molecular Biology and Biophysics SB RAMS, Novosibirsk, Timakova str., 2, 630117 (Russian Federation); Novosibirsk State University, Novosibirsk, Pirogova str., 2, 630090 (Russian Federation)

    2013-09-01

    Dichlorodiphenyltrichloroethane (DDT) is a widely used organochlorine pesticide and a xenoestrogen that promotes rodent hepatomegaly and tumours. A recent study has shown significant correlation between DDT serum concentration and liver cancer incidence in humans, but the underlying mechanisms remain elusive. We hypothesised that a mixture of DDT isomers could exert effects on the liver through pathways instead of classical ERs. The acute effects of a DDT mixture containing the two major isomers p,p′-DDT (85%) and o,p′-DDT (15%) on CAR and ERα receptors and their cell cycle and apoptosis target genes were studied in mouse livers. ChIP results demonstrated increased CAR and ERα recruitment to their specific target gene binding sites in response to the DDT mixture. The results of real-time RT-PCR were consistent with the ChIP data and demonstrated that the DDT was able to activate both CAR and ERα in mouse livers, leading to target gene transcriptional increases including Cyp2b10, Gadd45β, cMyc, Mdm2, Ccnd1, cFos and E2f1. Western blot analysis demonstrated increases in cell cycle progression proteins cMyc, Cyclin D1, CDK4 and E2f1 and anti-apoptosis proteins Mdm2 and Gadd45β. In addition, DDT exposure led to Rb phosphorylation. Increases in cell cycle progression and anti-apoptosis proteins were accompanied by a decrease in p53 content and its transcriptional activity. However, the DDT was unable to stimulate the β-catenin signalling pathway, which can play an important role in hepatocyte proliferation. Thus, our results indicate that DDT treatment may result in cell cycle progression and apoptosis inhibition through CAR- and ERα-mediated gene activation in mouse livers. These findings suggest that the proliferative and anti-apoptotic conditions induced by CAR and ERα activation may be important contributors to the early stages of hepatocarcinogenesis as produced by DDT in rodent livers. - Highlights: • DDT activated both CAR and ERα and their cell

  19. Approach of technical decision-making by element flow analysis and Monte-Carlo simulation of municipal solid waste stream

    Institute of Scientific and Technical Information of China (English)

    TIAN Bao-guo; SI Ji-tao; ZHAO Yan; WANG Hong-tao; HAO Ji-ming

    2007-01-01

    This paper deals with the procedure and methodology which can be used to select the optimal treatment and disposal technology of municipal solid waste (MSW), and to provide practical and effective technical support to policy-making, on the basis of study on solid waste management status and development trend in China and abroad. Focusing on various treatment and disposal technologies and processes of MSW, this study established a Monte-Carlo mathematical model of cost minimization for MSW handling subjected to environmental constraints. A new method of element stream (such as C, H, O, N, S) analysis in combination with economic stream analysis of MSW was developed. By following the streams of different treatment processes consisting of various techniques from generation, separation, transfer, transport, treatment, recycling and disposal of the wastes, the element constitution as well as its economic distribution in terms of possibility functions was identified. Every technique step was evaluated economically. The Mont-Carlo method was then conducted for model calibration. Sensitivity analysis was also carried out to identify the most sensitive factors. Model calibration indicated that landfill with power generation of landfill gas was economically the optimal technology at the present stage under the condition of more than 58% of C, H, O, N, S going to landfill. Whether or not to generate electricity was the most sensitive factor. If landfilling cost increases, MSW separation treatment was recommended by screening first followed with incinerating partially and composting partially with residue landfilling. The possibility of incineration model selection as the optimal technology was affected by the city scale. For big cities and metropolitans with large MSW generation, possibility for constructing large-scale incineration facilities increases, whereas, for middle and small cities, the effectiveness of incinerating waste decreases.

  20. Analysis of an attempt at detection of neutrons produced in a plasma discharge electrolytic cell

    CERN Document Server

    Widom, A; Srivastava, Y N

    2013-01-01

    R. Faccini et al. \\cite{Faccini:2013} have attempted a replication of an earlier experiment by D. Cirillo et al. \\cite{Cirillo:2012} in which neutrons [as well as nuclear transmutations] were observed in a modified Mizuno cell. No neutron production is observed in the recent experiment \\cite{Faccini:2013} and no evidence for microwave radiation or nuclear transmutations are reported. A careful analysis shows major technical differences in the two experiments and we explore the underlying reasons for the lack of any nuclear activity in the newer experiment.

  1. Fabrication, testing, and analysis of anisotropic carbon/glass hybrid composites: volume 1: technical report.

    Energy Technology Data Exchange (ETDEWEB)

    Wetzel, Kyle K. (Wetzel Engineering, Inc. Lawrence, Kansas); Hermann, Thomas M. (Wichita state University, Wichita, Kansas); Locke, James (Wichita state University, Wichita, Kansas)

    2005-11-01

    o} from the long axis for approximately two-thirds of the laminate volume (discounting skin layers), with reinforcing carbon fibers oriented axially comprising the remaining one-third of the volume. Finite element analysis of each laminate has been performed to examine first ply failure. Three failure criteria--maximum stress, maximum strain, and Tsai-Wu--have been compared. Failure predicted by all three criteria proves generally conservative, with the stress-based criteria the most conservative. For laminates that respond nonlinearly to loading, large error is observed in the prediction of failure using maximum strain as the criterion. This report documents the methods and results in two volumes. Volume 1 contains descriptions of the laminates, their fabrication and testing, the methods of analysis, the results, and the conclusions and recommendations. Volume 2 contains a comprehensive summary of the individual test results for all laminates.

  2. A technical, economic, and environmental analysis of energy production from newspaper in Ireland.

    Science.gov (United States)

    Murphy, J D; Power, N

    2007-01-01

    The production of newspaper corresponds to 37 kg per person per annum in Ireland. Newspaper becomes a waste product in a short period of time; only 13% of domestic waste paper is recycled (data on newspaper is not available). Four scenarios, which generate energy from newspaper, are analysed. These scenarios may be summarised as follows: lignocellulosic biomass conversion to ethanol (transport fuel); co-digestion with the organic fraction of municipal solid waste and production of CH4-enriched biogas (transport fuel); co-firing with the residue of municipal solid waste in an incinerator; and gasification of newspaper as a sole fuel. Two of the scenarios involve transport fuel production; two involve the production of electricity and heat. Two of the scenarios involve newspaper as the sole ingredient; two involve co- utilisation of newspaper with another waste stream. Assuming no economic market for heat, then only the transport scenarios have the potential to be economic; indeed the biogas scenario is shown to be extremely competitive generating a potential profit of euro 227/t newspaper. A greenhouse-gas analysis indicates that the biogas scenario generated the best net greenhouse-gas savings. However when a market for heat is available, gasification was shown to be most advantageous.

  3. Vehicle Lightweighting: 40% and 45% Weight Savings Analysis: Technical Cost Modeling for Vehicle Lightweighting

    Energy Technology Data Exchange (ETDEWEB)

    Mascarin, Anthony [Idaho National Lab. (INL), Idaho Falls, ID (United States); Hannibal, Ted [Idaho National Lab. (INL), Idaho Falls, ID (United States); Raghunathan, Anand [Idaho National Lab. (INL), Idaho Falls, ID (United States); Ivanic, Ziga [Idaho National Lab. (INL), Idaho Falls, ID (United States); Francfort, James [Idaho National Lab. (INL), Idaho Falls, ID (United States)

    2015-04-01

    The U.S. Department of Energy’s Vehicle Technologies Office, Materials area commissioned a study to model and assess manufacturing economics of alternative design and production strategies for a series of lightweight vehicle concepts. The strategic targets were a 40% and a 45% mass reduction relative to a standard North American midsize passenger sedan at an effective cost of $3.42 per pound (lb) saved. The baseline vehicle was an average of several available vehicles in this class. Mass and cost breakdowns from several sources were used, including original equipment manufacturers’ (OEMs’) input through U.S. Department of Energy’s Vehicle Technologies Office programs and public presentations, A2Mac1 LLC’s teardown information, Lotus Engineering Limited and FEV, Inc. breakdowns in their respective lightweighting studies, and IBIS Associates, Inc.’s decades of experience in automotive lightweighting and materials substitution analyses. Information on lightweighting strategies in this analysis came from these same sources and the ongoing U.S. Department of Energy-funded Vehma International of America, Inc. /Ford Motor Company Multi-Material Lightweight Prototype Vehicle Demonstration Project, the Aluminum Association Transportation Group, and many United States Council for Automotive Research’s/United States Automotive Materials Partnership LLC lightweight materials programs.

  4. Technical note: Headspace analysis of explosive compounds using a novel sampling chamber.

    Science.gov (United States)

    DeGreeff, Lauryn; Rogers, Duane A; Katilie, Christopher; Johnson, Kevin; Rose-Pehrsson, Susan

    2015-03-01

    The development of instruments and methods for explosive vapor detection is a continually evolving field of interest. A thorough understanding of the characteristic vapor signatures of explosive material is imperative for the development and testing of new and current detectors. In this research a headspace sampling chamber was designed to contain explosive materials for the controlled, reproducible sampling and characterization of vapors associated with these materials. In a detonation test, the chamber was shown to contain an explosion equivalent to three grams of trinitrotoluene (TNT) without damage to the chamber. The efficacy of the chamber in controlled headspace sampling was evaluated in laboratory tests with bulk explosive materials. Small quantities of TNT, triacetone triperoxide (TATP) and hexamethylene triperoxide diamine (HMTD) were separately placed in the sampling chamber, and the headspace of each material was analyzed by gas chromatography/mass spectrometry (GC/MS) with online cryogenic trapping to yield characteristic vapor signatures for each explosive compound. Chamber sampling conditions, temperature and sampling time, were varied to demonstrate suitability for precise headspace analysis.

  5. How measurement artifacts affect cerebral autoregulation outcomes: A technical note on transfer function analysis.

    Science.gov (United States)

    Meel-van den Abeelen, Aisha S S; de Jong, Daan L K; Lagro, Joep; Panerai, Ronney B; Claassen, Jurgen A H R

    2016-05-01

    Cerebral autoregulation (CA) is the mechanism that aims to maintain adequate cerebral perfusion during changes in blood pressure (BP). Transfer function analysis (TFA), the most reported method in literature to quantify CA, shows large between-study variability in outcomes. The aim of this study is to investigate the role of measurement artifacts in this variation. Specifically, the role of distortion in the BP and/or CBFV measurementon TFA outcomes was investigated. The influence of three types of artifacts on TFA outcomes was studied: loss of signal, motion artifacts, and baseline drifts. TFA metrics of signals without the simulated artifacts were compared with those of signals with artifacts. TFA outcomes scattered highly when more than 10% of BP signal or over 8% of the CBFV signal was lost, or when measurements contained one or more artifacts resulting from head movement. Furthermore, baseline drift affected interpretation of TFA outcomes when the power in the BP signal was 5 times the power in the LF band. In conclusion, loss of signal in BP and loss in CBFV, affects interpretation of TFA outcomes. Therefore, it is vital to validate signal quality to the defined standards before interpreting TFA outcomes.

  6. Area 3, SRC-II coal slurry preheater studies report for the technical data analysis program

    Energy Technology Data Exchange (ETDEWEB)

    1984-08-01

    This report reviews the raw data gathered from the Preheater B test runs at Ft. Lewis, and also the Preheater B results presented in the Solvent Refined Coal (SRC) Process Final Report, Volumes 1 and 2 of Slurry Preheater Design, SRC-II Process and the Ft. Lewis Slurry Preheater Data Analysis, 1 1/2 Inch Coil by Gulf Science and Technology Corporation of Pittsburgh, Pennsylvania. attempts were made to correlate several variables not previously considered with slurry viscosity and thermal conductivity. Only partial success was realized. However, in the process of attempting to correlate these variables an understanding of why some variables could not be correlated was achieved. An attempt was also made, using multiple linear regression, to correlate coal slurry viscosity and thermal conductivity with several independent variables among which were temperature, coal concentration, total solids, coal type, slurry residence time, shear rate, and unit size. The final correlations included some, but not all, of these independent variables. This report is not a stand alone document and should be considered a supplement to work already done. It should be read in conjunction with the reports referenced above.

  7. Technical Note: REFIR-PAD level 1 data analysis and performance characterization

    Directory of Open Access Journals (Sweden)

    G. Bianchini

    2008-07-01

    Full Text Available The outgoing long-wave radiation from the Earth's atmosphere in the far infrared spectral region is mostly unexplored, while is well recognized that the water vapour contribution to greenhouse trapping is dominant in this region. The Radiation Explorer in the Far InfraRed (REFIR study has proven the feasibility of a space-borne Fourier transform spectrometer able to perform the measurement in the 100–1100 cm−1 range with a resolution of 0.5 cm−1. Following this work a prototype of the spectrometer named REFIR-PAD (Prototype for Applications and Development has been developed to observe the atmospheric radiance from both ground-based sites and from stratospheric balloon platforms. In this work we describe the REFIR-PAD level 1 data analysis procedure, that, starting from raw instrumental data produces the calibrated atmospheric spectral radiance. Performances of the procedure are also described.

  8. Analysis of human factors effects on the safety of transporting radioactive waste materials: Technical report

    Energy Technology Data Exchange (ETDEWEB)

    Abkowitz, M.D.; Abkowitz, S.B.; Lepofsky, M.

    1989-04-01

    This report examines the extent of human factors effects on the safety of transporting radioactive waste materials. It is seen principally as a scoping effort, to establish whether there is a need for DOE to undertake a more formal approach to studying human factors in radioactive waste transport, and if so, logical directions for that program to follow. Human factors effects are evaluated on driving and loading/transfer operations only. Particular emphasis is placed on the driving function, examining the relationship between human error and safety as it relates to the impairment of driver performance. Although multi-modal in focus, the widespread availability of data and previous literature on truck operations resulted in a primary study focus on the trucking mode from the standpoint of policy development. In addition to the analysis of human factors accident statistics, the report provides relevant background material on several policies that have been instituted or are under consideration, directed at improving human reliability in the transport sector. On the basis of reported findings, preliminary policy areas are identified. 71 refs., 26 figs., 5 tabs.

  9. Genetic Analysis of Snake River Sockeye Salmon (Oncorhynchus Nerka), 2003 Technical Report.

    Energy Technology Data Exchange (ETDEWEB)

    Faler, Joyce; Powell, Madison

    2003-12-01

    A total of 1720 Oncorhynchus nerka tissue samples from 40 populations were characterized using mitochondrial DNA RFLPs (Restriction Fragment Length Polymorphisms). Analysis of anadromous sockeye populations indicated the historical presence of four major maternal lineages. Thirty-five composite mitochondrial haplotypes were observed from the 40 populations of O. nerka sampled throughout the Pacific Northwest. Six of these composite haplotypes ranged in frequency from 7-26% overall and were commonly observed in most populations. The six haplotypes together comprised 90% of the sampled O. nerka. An average of 4.6 composite haplotypes were observed per population. Genetic markers used were satisfactory in separating Redfish Lake anadromous sockeye, residual sockeye and outmigrants from the sympatric kokanee population that spawns in the Fishhook Creek tributary. Outmigrants appear to be primarily composed of progeny from resident residual sockeye, and captively-reared progeny of the captive broodstock program. Thus, residual sockeye may be considered a suitable source of genetic variation to maintain genetic diversity among captive broodstocks of anadromous sockeye. Fishhook Creek kokanee are genetically diverse and during spawning, are temporally and spatially isolated from the residual sockeye population. Eleven composite haplotypes were observed in the kokanee population. The unusually high number of haplotypes is most likely a consequence of periodic stocking of Redfish Lake with kokanee from other sources. Genetic data from Redfish Lake creel samples taken during 1996-1999 putatively indicate the incidental take of a listed resident sockeye.

  10. Technical note: Sampling and processing of mesocosm sediment trap material for quantitative biogeochemical analysis

    Science.gov (United States)

    Boxhammer, Tim; Bach, Lennart T.; Czerny, Jan; Riebesell, Ulf

    2016-05-01

    Sediment traps are the most common tool to investigate vertical particle flux in the marine realm. However, the spatial and temporal decoupling between particle formation in the surface ocean and particle collection in sediment traps at depth often handicaps reconciliation of production and sedimentation even within the euphotic zone. Pelagic mesocosms are restricted to the surface ocean, but have the advantage of being closed systems and are therefore ideally suited to studying how processes in natural plankton communities influence particle formation and settling in the ocean's surface. We therefore developed a protocol for efficient sample recovery and processing of quantitatively collected pelagic mesocosm sediment trap samples for biogeochemical analysis. Sedimented material was recovered by pumping it under gentle vacuum through a silicon tube to the sea surface. The particulate matter of these samples was subsequently separated from bulk seawater by passive settling, centrifugation or flocculation with ferric chloride, and we discuss the advantages and efficiencies of each approach. After concentration, samples were freeze-dried and ground with an easy to adapt procedure using standard lab equipment. Grain size of the finely ground samples ranged from fine to coarse silt (2-63 µm), which guarantees homogeneity for representative subsampling, a widespread problem in sediment trap research. Subsamples of the ground material were perfectly suitable for a variety of biogeochemical measurements, and even at very low particle fluxes we were able to get a detailed insight into various parameters characterizing the sinking particles. The methods and recommendations described here are a key improvement for sediment trap applications in mesocosms, as they facilitate the processing of large amounts of samples and allow for high-quality biogeochemical flux data.

  11. Constraining shallow seismic event depth via synthetic modeling for Expert Technical Analysis at the IDC

    Science.gov (United States)

    Stachnik, J.; Rozhkov, M.; Baker, B.; Bobrov, D.; Friberg, P. A.

    2015-12-01

    Depth of event is an important criterion of seismic event screening at the International Data Center, CTBTO. However, a thorough determination of the event depth can be conducted mostly through special analysis because the IDC's Event Definition Criteria is based, in particular, on depth estimation uncertainties. This causes a large number of events in the Reviewed Event Bulletin to have depth constrained to the surface. When the true origin depth is greater than that reasonable for a nuclear test (3 km based on existing observations), this may result in a heavier workload to manually distinguish between shallow and deep events. Also, IDC depth criterion is not applicable to the events with the small t(pP-P) travel time difference, which is the case of the nuclear test. Since the shape of the first few seconds of signal of very shallow events is very sensitive to the presence of the depth phase, cross correlation between observed and theoretic seismogram can provide an estimate for the depth of the event, and so provide an expansion to the screening process. We exercised this approach mostly with events at teleseismic and partially regional distances. We found that such approach can be very efficient for the seismic event screening process, with certain caveats related mostly to the poorly defined crustal models at source and receiver which can shift the depth estimate. We used adjustable t* teleseismic attenuation model for synthetics since this characteristic is not determined for most of the rays we studied. We studied a wide set of historical records of nuclear explosions, including so called Peaceful Nuclear Explosions (PNE) with presumably known depths, and recent DPRK nuclear tests. The teleseismic synthetic approach is based on the stationary phase approximation with Robert Herrmann's hudson96 program, and the regional modelling was done with the generalized ray technique by Vlastislav Cerveny modified to the complex source topography.

  12. Technical improvements in the DNA analysis of the myotonic dystrophy (DM) mutation

    Energy Technology Data Exchange (ETDEWEB)

    Leblond, S.; Lehev, D.; Barcelo, J. [Children`s Hospital of Eastern Ontario, Ottawa (Canada)] [and others

    1994-09-01

    It has become increasingly clear that widespread clinical application of routine DNA diagnosis requires robust and easily replicated methodologies. Mutation analysis in DM involves detection of a CTG expansion which may increase in size between generations within a family. DNA testing has required two distinct methods: genomic and PCR DNA Southern blotting. Genomic Southerns visualize from E1 (hundreds of repeats) to the very largest E4 (thousands of repeats in congenital DM). PCR Southerns permit detection of the smallest mutations (E0, protomutations associated with minimal if any clinical signs) to E3, but E4 is not uniformly visualized. In order to improve the DM testing such that even the largest expansions are visualized by a single PCR test, we have altered the PCR conditions. Since the PCR conditions do not substantially affect the normal allele of less than 200 bp, CTG expansion must be directly monitored by hybridization with a labelled (CTG){sub 10} oligonucleotide. Unlike PCR of the CGG expansion in fragile X, addition of deazaGTP reduced visualization of the DM expansion. Addition of single-stranded protein and DMSO significantly improved PCR up to ten-fold such that E4s were visualized. The CTG expansion was very sensitive to the denaturing cycle temperature (which does not affect the intensity of the normal allele). Thus, 96{degrees}C on the Perkin Elmer 480 was optimal in our hands, with 98{degrees}C and 94{degrees}C actually causing loss of even the intermediate sized E1 and E2 expansions. This has implications when setting up the DM test on different thermocyclers where digital readings may not reflect actual block temperature. These PCR `tune-ups` will support more reliable and streamlined analyses, as more expansion mutations are recognized and routinely offered for clinical use.

  13. Financial performance monitoring of the technical efficiency of critical access hospitals: a data envelopment analysis and logistic regression modeling approach.

    Science.gov (United States)

    Wilson, Asa B; Kerr, Bernard J; Bastian, Nathaniel D; Fulton, Lawrence V

    2012-01-01

    From 1980 to 1999, rural designated hospitals closed at a disproportionally high rate. In response to this emergent threat to healthcare access in rural settings, the Balanced Budget Act of 1997 made provisions for the creation of a new rural hospital--the critical access hospital (CAH). The conversion to CAH and the associated cost-based reimbursement scheme significantly slowed the closure rate of rural hospitals. This work investigates which methods can ensure the long-term viability of small hospitals. This article uses a two-step design to focus on a hypothesized relationship between technical efficiency of CAHs and a recently developed set of financial monitors for these entities. The goal is to identify the financial performance measures associated with efficiency. The first step uses data envelopment analysis (DEA) to differentiate efficient from inefficient facilities within a data set of 183 CAHs. Determining DEA efficiency is an a priori categorization of hospitals in the data set as efficient or inefficient. In the second step, DEA efficiency is the categorical dependent variable (efficient = 0, inefficient = 1) in the subsequent binary logistic regression (LR) model. A set of six financial monitors selected from the array of 20 measures were the LR independent variables. We use a binary LR to test the null hypothesis that recently developed CAH financial indicators had no predictive value for categorizing a CAH as efficient or inefficient, (i.e., there is no relationship between DEA efficiency and fiscal performance).

  14. Analysis of Aviation Safety Reporting System Incident Data Associated with the Technical Challenges of the Atmospheric Environment Safety Technology Project

    Science.gov (United States)

    Withrow, Colleen A.; Reveley, Mary S.

    2014-01-01

    This study analyzed aircraft incidents in the NASA Aviation Safety Reporting System (ASRS) that apply to two of the three technical challenges (TCs) in NASA's Aviation Safety Program's Atmospheric Environment Safety Technology Project. The aircraft incidents are related to airframe icing and atmospheric hazards TCs. The study reviewed incidents that listed their primary problem as weather or environment-nonweather between 1994 and 2011 for aircraft defined by Federal Aviation Regulations (FAR) Parts 121, 135, and 91. The study investigated the phases of flight, a variety of anomalies, flight conditions, and incidents by FAR part, along with other categories. The first part of the analysis focused on airframe-icing-related incidents and found 275 incidents out of 3526 weather-related incidents over the 18-yr period. The second portion of the study focused on atmospheric hazards and found 4647 incidents over the same time period. Atmospheric hazards-related incidents included a range of conditions from clear air turbulence and wake vortex, to controlled flight toward terrain, ground encounters, and incursions.

  15. Modern technical solutions of gas-fired heating devices of household and communal use and analysis of their testing

    Energy Technology Data Exchange (ETDEWEB)

    Bodzon, L.; Radwan, W. [Oil Mining and Gas Engineering Institute, Cracow (Poland)

    1995-12-31

    A review of technical solutions for gas-fired heating devices for household and communal use in Poland is presented. Based upon the analysis it is stated that the power output of Polish and foreign boilers ranges between 9 and 35 kW. The carbon monoxide content in flue gases reaches (on average) 0.005 vol.%, i.e., it is much lower than the maximum permissible level. Temperature of flue gases (excluding condensation boilers and those with air-tight combustion chamber) ranges between 150 and 200{degrees}C and their heating efficiency reaches 87-93%. The best parameters are given for condensation boilers, however they are still not widespread in Poland for the high cost of the equipment and assembling works. Among the heaters, the most safe are convection devices with closed combustion chamber; their efficiency is also the highest. Thus, it is concluded that a wide spectrum of high efficiency heating devices with good combustion parameters are available. The range of output is sufficient to meet household and communal requirement. They are however - predominantly - units manufactured abroad. It is difficult to formulate the program aimed at the improvement of the technique of heating devices made in Poland, and its implementation is uncertain because the production process is broken up into small handicraft workshops.

  16. Time-resolved ICP-MS measurement: a new method for elemental and multiparametric analysis of single cells.

    Science.gov (United States)

    Miyashita, Shin-ichi; Groombridge, Alexander S; Fujii, Shin-ichiro; Takatsu, Akiko; Chiba, Koichi; Inagaki, Kazumi

    2014-01-01

    Time-resolved inductively coupled plasma mass spectrometry (ICP-MS) has attracted much attention for elemental and multiparametric analysis of single cells, instead of a classical bulk analysis of large amount of cells after a dissolution. In the time-resolved measurement, cells are directly introduced into the plasma via nebulizing or micro drop dispensing, and then ion plumes corresponding to single cells are individually detected with a high time resolution. The sensitivity and cell throughput in the measurement strongly depend on the time resolution. A high cell introduction efficiency into the plasma supports for a reduction of cell consumption. Biomolecules can also be measured through the attachment of elemental tags, and then the amount distribution of elements and biomolecules in single cells can be evaluated, while providing information concerning cell-to-cell variations. By applying ICP time-of-flight mass spectrometry (ICP-TOFMS), multiparametric analysis of elements and biomolecules can be achieved similar to that by a flow cytometer. This article highlights the technical aspects of the time-resolved ICP-MS measurement technique for elemental and multiparametric analysis of single cells.

  17. Thermodynamic analysis of biofuels as fuels for high temperature fuel cells

    Directory of Open Access Journals (Sweden)

    Milewski Jarosław

    2013-02-01

    Full Text Available Based on mathematical modeling and numerical simulations, applicativity of various biofuels on high temperature fuel cell performance are presented. Governing equations of high temperature fuel cell modeling are given. Adequate simulators of both solid oxide fuel cell (SOFC and molten carbonate fuel cell (MCFC have been done and described. Performance of these fuel cells with different biofuels is shown. Some characteristics are given and described. Advantages and disadvantages of various biofuels from the system performance point of view are pointed out. An analysis of various biofuels as potential fuels for SOFC and MCFC is presented. The results are compared with both methane and hydrogen as the reference fuels. The biofuels are characterized by both lower efficiency and lower fuel utilization factors compared with methane. The presented results are based on a 0D mathematical model in the design point calculation. The governing equations of the model are also presented. Technical and financial analysis of high temperature fuel cells (SOFC and MCFC are shown. High temperature fuel cells can be fed by biofuels like: biogas, bioethanol, and biomethanol. Operational costs and possible incomes of those installation types were estimated and analyzed. A comparison against classic power generation units is shown. A basic indicator net present value (NPV for projects was estimated and commented.

  18. Thermodynamic analysis of biofuels as fuels for high temperature fuel cells

    Science.gov (United States)

    Milewski, Jarosław; Bujalski, Wojciech; Lewandowski, Janusz

    2013-02-01

    Based on mathematical modeling and numerical simulations, applicativity of various biofuels on high temperature fuel cell performance are presented. Governing equations of high temperature fuel cell modeling are given. Adequate simulators of both solid oxide fuel cell (SOFC) and molten carbonate fuel cell (MCFC) have been done and described. Performance of these fuel cells with different biofuels is shown. Some characteristics are given and described. Advantages and disadvantages of various biofuels from the system performance point of view are pointed out. An analysis of various biofuels as potential fuels for SOFC and MCFC is presented. The results are compared with both methane and hydrogen as the reference fuels. The biofuels are characterized by both lower efficiency and lower fuel utilization factors compared with methane. The presented results are based on a 0D mathematical model in the design point calculation. The governing equations of the model are also presented. Technical and financial analysis of high temperature fuel cells (SOFC and MCFC) are shown. High temperature fuel cells can be fed by biofuels like: biogas, bioethanol, and biomethanol. Operational costs and possible incomes of those installation types were estimated and analyzed. A comparison against classic power generation units is shown. A basic indicator net present value (NPV) for projects was estimated and commented.

  19. Technical Note: Statistical analysis of defects of tiles´ joints

    Directory of Open Access Journals (Sweden)

    de Brito, J.

    2007-03-01

    Full Text Available The present article addresses tile joint defects in interior and exterior floors and walls. It begins with a classification of tile joint defects along with their likely causes, establishing a correlation matrix between the two sets of parameters. This is followed by the identification of the flaws found most frequently in this type of cladding, based on field data collected during 88 inspections of tile joint material. The statistical analysis of these data is shown in the form of charts representing the frequency of joint defects, their causes and the interrelationships between the two. The field work confirmed the high frequency of joint defects in all types of tiling. This conclusion underscores the need for proper joint design and maintenance planning (including periodic inspection and repair as appropriate to guarantee the durability of this type of cladding.En este trabajo se analizan los defectos de junta que pueden aparecer en las distintas aplicaciones cerámicas actuales. En primer lugar, se propone una clasificación tanto de los defectos como de las causas más probables de los mismos. A continuación se presenta una matriz de correlación de dichos defectos y causas probables. A partir de 88 inspecciones de paramentos cerámicos, se han identificado los defectos del material de juntas más frecuentes, describiéndose los resultados del análisis estadístico de los datos recogidos con motivo de dichas inspecciones. En los cuadros correspondientes se especifican la frecuencia del defecto, las causas observadas y la relación entre ambas en la muestra. Los datos recogidos in situ evidencian una alta incidencia de defectos del material de juntas en esta clase de soluciones cerámicas. Dicha conclusión constata la necesidad de actuaciones preventivas, que deberían incluir el correcto diseño de las juntas cerámicas y la planificación previa de los trabajos de mantenimiento (con visitas periódicas y tareas de reparación. Se

  20. Evaluation of preparation methods for MS-based analysis of intestinal epithelial cell proteomes

    DEFF Research Database (Denmark)

    Hesselager, Marianne Overgaard; Codrea, Marius Cosmin; Bendixen, Emøke

    2015-01-01

    The gut epithelium formed between an organism and the environment plays an essential role in host–microbe interactions, yet remains one of the least characterized mammalian tissues. Especially the membrane proteins, which are critical to bacterial adhesion, are understudied, because these proteins...... are low in abundance, and large amounts of sample is needed for their preparation and for undertaking MS-based analysis. The aim of this study was to evaluate three different methods for isolation and preparation of pig intestinal epithelial cells for MS-based analysis of the proteome. Samples were...... of ease and speed of sample preparation, as well as protein recovery. In comparison, more gentle methods where intestinal epithelial cells are harvested by shaking are more time consuming, result in lower protein yield, and are prone to increased technical variation due to multiple steps involved....

  1. Detailed interrogation of trypanosome cell biology via differential organelle staining and automated image analysis

    Directory of Open Access Journals (Sweden)

    Wheeler Richard J

    2012-01-01

    Full Text Available Abstract Background Many trypanosomatid protozoa are important human or animal pathogens. The well defined morphology and precisely choreographed division of trypanosomatid cells makes morphological analysis a powerful tool for analyzing the effect of mutations, chemical insults and changes between lifecycle stages. High-throughput image analysis of micrographs has the potential to accelerate collection of quantitative morphological data. Trypanosomatid cells have two large DNA-containing organelles, the kinetoplast (mitochondrial DNA and nucleus, which provide useful markers for morphometric analysis; however they need to be accurately identified and often lie in close proximity. This presents a technical challenge. Accurate identification and quantitation of the DNA content of these organelles is a central requirement of any automated analysis method. Results We have developed a technique based on double staining of the DNA with a minor groove binding (4'', 6-diamidino-2-phenylindole (DAPI and a base pair intercalating (propidium iodide (PI or SYBR green fluorescent stain and color deconvolution. This allows the identification of kinetoplast and nuclear DNA in the micrograph based on whether the organelle has DNA with a more A-T or G-C rich composition. Following unambiguous identification of the kinetoplasts and nuclei the resulting images are amenable to quantitative automated analysis of kinetoplast and nucleus number and DNA content. On this foundation we have developed a demonstrative analysis tool capable of measuring kinetoplast and nucleus DNA content, size and position and cell body shape, length and width automatically. Conclusions Our approach to DNA staining and automated quantitative analysis of trypanosomatid morphology accelerated analysis of trypanosomatid protozoa. We have validated this approach using Leishmania mexicana, Crithidia fasciculata and wild-type and mutant Trypanosoma brucei. Automated analysis of T. brucei

  2. Infrastructure of Baltic Region Transmission System: Analysis of Technical and Economic Factors of its Development

    Science.gov (United States)

    Obushevs, A.; Oleinikova, I.; Mutule, A.

    2014-08-01

    The operational conditions of new networks dictate new requirements for the transmission planning, which would include the electricity market figures and a sizable involvement of renewable generation. This paper focuses on the transmission expansion planning techniques based on the calculations of optimal power flows and on the concept of development planning and sustainability. A description is given for the mathematical model of calculations and analysis of transmission system. The results have shown that the Baltic transmission system infrastructure can successfully be analyzed based on the proposed methodology and developed mathematical model Baltijas valstu (Latvijas, Lietuvas un Igaunijas) energosistēmas ir cieši saistītas vēsturiski, un to darbība nav iespējama bez savstarpējas sadarbības attīstības un darba režīmu jautājumos. Ekonomisko attiecību īstenošanu enerģētikas sektorā paātrināja elektroenerģijas tirgus attīstība. Baltijas valstu enerģētikas politika ir integrēta ES enerģētikas stratēģijas sastāvdaļa, nosakot trīs galvenos mērķus: enerģētikas nozares konkurētspēja, ilgtspējīga attīstība un drošība. Visas trīs Baltijas energosistēmas veica lielu darba apjomu iekārtu modernizācijā un standartu saskaņošanā, kuras ir saskaņā ar Eiropas Savienības prasībām, kā arī par tirgus attiecību un tehnoloģiju standartu ieviešanu, lai nodrošinātu energoapgādes drošību un elektroenerģijas pieejamību patērētājiem Tomēr, ņemot vērā strauji mainīgos ārējos apstākļus, it īpaši ģeopolitiskos faktorus, Baltijas valstu enerģētikas politika būtu jāizskata ar mērķi novērtēt, kā šie faktori ietekmē energosistēmas ilgtspējīgu attīstību kopumā. No iepriekš minētā izriet, ka nepieciešama jauna nacionāla enerģētikas stratēģija, kura stiprinātu efektīvu ekonomisko un sociālo pamatu ilgtspējīgu attīstību Baltijas valstu nacionālā ekonomikā. Šī darba m

  3. Single cell array impedance analysis in a microfluidic device

    Science.gov (United States)

    Altinagac, Emre; Taskin, Selen; Kizil, Huseyin

    2016-10-01

    Impedance analysis of single cells is presented in this paper. Following the separation of a target cell type by dielectrophoresis in our previous work, this paper focuses on capturing the cells as a single array and performing impedance analysis to point out the signature difference between each cell type. Lab-on-a-chip devices having a titanium interdigitated electrode layer on a glass substrate and a PDMS microchannel are fabricated to capture each cell in a single form and perform impedance analysis. HCT116 (homosapiens colon colorectal carcin) and HEK293 (human embryonic kidney) cells are used in our experiments.

  4. Congener-specific carbon isotopic analysis of technical PCB and PCN mixtures using two-dimensional gas chromatography-isotope ratio mass spectrometry.

    Science.gov (United States)

    Horii, Yuichi; Kannan, Kurunthachalam; Petrick, Gert; Gamo, Toshitaka; Falandysz, Jerzy; Yamashita, Nobuyoshi

    2005-06-01

    Analysis of stable carbon isotope fractionation is a useful method to study the sources and fate of anthropogenic organic contaminants such as polychlorinated biphenyls (PCBs) in the environment. To evaluate the utility of carbon isotopes, determination of isotopic ratios of 13C/12C in source materials, for example, technical PCB preparations, is needed. In this study, we determined delta13C values of 31 chlorobiphenyl (CB) congeners in 18 technical PCB preparations and 15 chloronaphthalene (CN) congeners in 6 polychlorinated naphthalene preparations using two-dimensional gas chromatography-combustion furnace-isotope ratio mass spectrometry (2DGC-C-IRMS). Development of 2DGC-IRMS enabled improved resolution and sensitivity of compound-specific carbon isotope analysis (CSIA) of CB or CN congeners. Delta13C values of PCB congeners ranged from -34.4 (Delors) to -22.0/1000 (Sovol). Analogous PCB preparations with similar chlorine content, but different geographical origin, had different delta13C values. PCB preparations from Eastern European countries--Delors, Sovol, Trichlorodiphenyl, and Chlorofen--had distinct delta13C values. PCB mixtures showed increased 13C depletion with increasing chlorine content. Delta13C values for individual CB congeners varied depending on the degree of chlorination in technical mixtures. Delta13C values of CN congeners in Halowaxes ranged from -26.3 to -21.7/1000 and these values are within the ranges observed for PCBs. This study establishes the range of delta13C values in technical PCB and PCN preparations, which may prove to be useful in the determination of sources of these compounds in the environment. This is the first study to employ 2DGC-IRMS analysis of delta13C values in technical PCB and PCN preparations.

  5. Technical Update: Johnson Space Center system using a solid electrolytic cell in a remote location to measure oxygen fugacities in CO/CO2 controlled-atmosphere furnaces

    Science.gov (United States)

    Jurewicz, A. J. G.; Williams, R. J.; Le, L.; Wagstaff, J.; Lofgren, G.; Lanier, A.; Carter, W.; Roshko, A.

    1993-01-01

    Details are given for the design and application of a (one atmosphere) redox-control system. This system differs from that given in NASA Technical Memorandum 58234 in that it uses a single solid-electrolytic cell in a remote location to measure the oxygen fugacities of multiple CO/CO2 controlled-atmosphere furnaces. This remote measurement extends the range of sample-furnace conditions that can be measured using a solid-electrolytic cell, and cuts costs by extending the life of the sensors and by minimizing the number of sensors in use. The system consists of a reference furnace and an exhaust-gas manifold. The reference furnace is designed according to the redox control system of NASA Technical Memorandum 58234, and any number of CO/CO2 controlled-atmosphere furnaces can be attached to the exhaust-gas manifold. Using the manifold, the exhaust gas from individual CO/CO2 controlled atmosphere furnaces can be diverted through the reference furnace, where a solid-electrolyte cell is used to read the ambient oxygen fugacity. The oxygen fugacity measured in the reference furnace can then be used to calculate the oxygen fugacity in the individual CO/CO2 controlled-atmosphere furnace. A BASIC computer program was developed to expedite this calculation.

  6. Fuel Cell Technology Status Analysis Project: Partnership Opportunities

    Energy Technology Data Exchange (ETDEWEB)

    2017-03-13

    Fact sheet describing the National Renewable Energy Laboratory's (NREL's) Fuel Cell Technology Status Analysis Project. NREL is seeking fuel cell industry partners from the United States and abroad to participate in an objective and credible analysis of commercially available fuel cell products to benchmark the current state of the technology and support industry growth.

  7. Gene set enrichment analysis and ingenuity pathway analysis of metastatic clear cell renal cell carcinoma cell line.

    Science.gov (United States)

    Khan, Mohammed I; Dębski, Konrad J; Dabrowski, Michał; Czarnecka, Anna M; Szczylik, Cezary

    2016-08-01

    In recent years, genome-wide RNA expression analysis has become a routine tool that offers a great opportunity to study and understand the key role of genes that contribute to carcinogenesis. Various microarray platforms and statistical approaches can be used to identify genes that might serve as prognostic biomarkers and be developed as antitumor therapies in the future. Metastatic renal cell carcinoma (mRCC) is a serious, life-threatening disease, and there are few treatment options for patients. In this study, we performed one-color microarray gene expression (4×44K) analysis of the mRCC cell line Caki-1 and the healthy kidney cell line ASE-5063. A total of 1,921 genes were differentially expressed in the Caki-1 cell line (1,023 upregulated and 898 downregulated). Gene Set Enrichment Analysis (GSEA) and Ingenuity Pathway Analysis (IPA) approaches were used to analyze the differential-expression data. The objective of this research was to identify complex biological changes that occur during metastatic development using Caki-1 as a model mRCC cell line. Our data suggest that there are multiple deregulated pathways associated with metastatic clear cell renal cell carcinoma (mccRCC), including integrin-linked kinase (ILK) signaling, leukocyte extravasation signaling, IGF-I signaling, CXCR4 signaling, and phosphoinositol 3-kinase/AKT/mammalian target of rapamycin signaling. The IPA upstream analysis predicted top transcriptional regulators that are either activated or inhibited, such as estrogen receptors, TP53, KDM5B, SPDEF, and CDKN1A. The GSEA approach was used to further confirm enriched pathway data following IPA.

  8. Onboard Hydrogen/Helium Sensors in Support of the Global Technical Regulation: An Assessment of Performance in Fuel Cell Electric Vehicle Crash Tests

    Energy Technology Data Exchange (ETDEWEB)

    Post, M. B.; Burgess, R.; Rivkin, C.; Buttner, W.; O' Malley, K.; Ruiz, A.

    2012-09-01

    Automobile manufacturers in North America, Europe, and Asia project a 2015 release of commercial hydrogen fuel cell powered light-duty road vehicles. These vehicles will be for general consumer applications, albeit initially in select markets but with much broader market penetration expected by 2025. To assure international harmony, North American, European, and Asian regulatory representatives are striving to base respective national regulations on an international safety standard, the Global Technical Regulation (GTR), Hydrogen Fueled Vehicle, which is part of an international agreement pertaining to wheeled vehicles and equipment for wheeled vehicles.

  9. TECHNICAL COORDINATION

    CERN Multimedia

    A. Ball

    2010-01-01

    Operational Experience At the end of the first full-year running period of LHC, CMS is established as a reliable, robust and mature experiment. In particular common systems and infrastructure faults accounted for <0.6 % CMS downtime during LHC pp physics. Technical operation throughout the entire year was rather smooth, the main faults requiring UXC access being sub-detector power systems and rack-cooling turbines. All such problems were corrected during scheduled technical stops, in the shadow of tunnel access needed by the LHC, or in negotiated accesses or access extensions. Nevertheless, the number of necessary accesses to the UXC averaged more than one per week and the technical stops were inevitably packed with work packages, typically 30 being executed within a few days, placing a high load on the coordination and area management teams. It is an appropriate moment for CMS Technical Coordination to thank all those in many CERN departments and in the Collaboration, who were involved in CMS techni...

  10. TECHNICAL COORDINATION

    CERN Multimedia

    A. Ball

    2010-01-01

    Operational Experience Since the closure of the detector in February, the technical operation of CMS has been quite smooth and reliable. Some minor interventions in UXC were required to cure failures of power supplies, fans, readout boards and rack cooling connections, but all these failures were repaired in scheduled technical stops or parasitically during access dedicated to fixing LHC technical problems. The only occasion when CMS had to request an access between fills was to search for the source of an alarm from the leak-detection cables mounted in the DT racks. After a few minutes of diagnostic search, a leaking air-purge was found. Replacement was complete within 2 hours. This incident demonstrated once more the value of these leak detection cables; the system will be further extended (during the end of year technical stop) to cover more racks in UXC and the floor beneath the detector. The magnet has also been operating reliably and reacted correctly to the 14s power cut on 29 May (see below). In or...

  11. Technical Training: Technical Training Seminar

    CERN Multimedia

    2004-01-01

    TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch Monday 9 February 2004 From 10:00 to 12:00 - IT Auditorium - bldg. 31, 3rd floor ANSOFT High-Frequency Seminar David Prestaux, Application Engineer, ANSOFT F-78535 BUC, France This Technical Training seminar will present two Ansoft application products: Ansoft HFSS and Ansoft Designer. Ansoft HFSS makes use of the Finite Element Method (FEM) to calculate field solutions from first principles. It can accurately predict all high-frequency behaviours such as dispersion, mode conversion, and losses due to materials and radiation. Ansoft Designer is a suite of design tools to fully integrate high-frequency, physics-based electromagnetic simulations into a seamless system-level simulation environment. Ansoft Designer uses a simple interface to give complete control over every design task, by a method allowing multiple solvers, Solver on Demand. • Introduction • Overview of the Ansoft Total solution • Ansoft HFSS 9...

  12. Analysis of fuel cell hybrid locomotives

    Science.gov (United States)

    Miller, Arnold R.; Peters, John; Smith, Brian E.; Velev, Omourtag A.

    Led by Vehicle Projects LLC, an international industry-government consortium is developing a 109 t, 1.2 MW road-switcher locomotive for commercial and military railway applications. As part of the feasibility and conceptual-design analysis, a study has been made of the potential benefits of a hybrid power plant in which fuel cells comprise the prime mover and a battery or flywheel provides auxiliary power. The potential benefits of a hybrid power plant are: (i) enhancement of transient power and hence tractive effort; (ii) regenerative braking; (iii) reduction of capital cost. Generally, the tractive effort of a locomotive at low speed is limited by wheel adhesion and not by available power. Enhanced transient power is therefore unlikely to benefit a switcher locomotive, but could assist applications that require high acceleration, e.g. subway trains with all axles powered. In most cases, the value of regeneration in locomotives is minimal. For low-speed applications such as switchers, the available kinetic energy and the effectiveness of traction motors as generators are both minimal. For high-speed heavy applications such as freight, the ability of the auxiliary power device to absorb a significant portion of the available kinetic energy is low. Moreover, the hybrid power plant suffers a double efficiency penalty, namely, losses occur in both absorbing and then releasing energy from the auxiliary device, which result in a net storage efficiency of no more than 50% for present battery technology. Capital cost in some applications may be reduced. Based on an observed locomotive duty cycle, a cost model shows that a hybrid power plant for a switcher may indeed reduce capital cost. Offsetting this potential benefit are the increased complexity, weight and volume of the power plant, as well as 20-40% increased fuel consumption that results from lower efficiency. Based on this analysis, the consortium has decided to develop a pure fuel cell road-switcher locomotive

  13. Cost-Benefit Analysis of a Bridge to Integrate the Management of Technical Information for Producing Technical Manuals and Training Courses

    Science.gov (United States)

    2010-11-01

    Philadelphia, and all Computer-Based Training (CBT) courses delivered by Navy eLearning (NeL), a part of the Naval Education and Training Command (NETC...Navy Learning Centers • Appendix E. Content Hours and Computer-Based Training (CBT) Courses Deli- vered by Navy eLearning (NeL) 1-3 C. Current Non...Analysis Record NAVSEA Naval Sea Systems Command NAVSSES Naval Ship Systems Engineering Station NeL Navy eLearning NETC Naval Education and Training

  14. Data Analysis for ARRA Early Fuel Cell Market Demonstrations (Presentation)

    Energy Technology Data Exchange (ETDEWEB)

    Kurtz, J.; Wipke, K.; Sprik, S.; Ramsden, T.

    2010-05-01

    Presentation about ARRA Early Fuel Cell Market Demonstrations, including an overview of the ARRE Fuel Cell Project, the National Renewable Energy Laboratory's data analysis objectives, deployment composite data products, and planned analyses.

  15. The Impact of Financial Crisis on the Predictability of the Stock Markets of PIGS Countries – Comparative Study of Prediction Accuracy of Technical Analysis and Neural Networks

    Directory of Open Access Journals (Sweden)

    Katarína Hiľovská

    2011-09-01

    Full Text Available To a degree the financial crisis influenced all European countries but the most affected are the PIGS (Portugal, Ireland, Greece and Spain. We investigated the effect of the financial crisis on the prediction accuracy of artificial neural networks on the Portuguese, Irish, Athens and Madrid Stock Exchange. We applied three-layered feed-forward neural networks with backpropagation algorithm to forecast the next day prices and we compared the paper returns achieved before and after the recent financial crisis. This method failed in forecasting the direction of the next day price movement but performed well in absolute price changes. However, it achieved better results than the strategy based on technical analysis in the period before the crisis. On the other hand, technical analysis performed better during the crisis.

  16. Nationwide survey of glandular tissue doses for the establishment of a breast cancer screening system using mammography. Analysis of technical parameters affecting the glandular tissue dose

    Energy Technology Data Exchange (ETDEWEB)

    Higashida, Yoshiharu [Kyushu Univ., Fukuoka (Japan). School of Health Sciences; Hagiwara, Akira; Asahara, Masaki [and others

    2000-09-01

    The effects of technical parameters, such as screen-film combinations, antiscatter grids, processing conditions and X-ray beam qualities, on the average glandular tissue dose of radiation were analyzed under two exposure conditions: The standard radiographic condition for an RMI 156 phantom at each facility (Standard Radiographic Condition). The radiographic condition needed to obtain a radiographic density of 1.20 to 1.40 with an RMI 156 phantom (Canonical Radiographic Condition). The analysis included 208 dose measurements at 104 facilities. The results showed that the average glandular tissue dose depends strongly on the grid types and screen-film combinations used. There were also significant differences in radiographic density between the Standard Radiographic Condition and the Canonical Radiographic Condition. This analysis of the technical parameters affecting the glandular tissue dose will be useful in establishing a standardized mammographic breast cancer screening system. (author)

  17. Thematic outlook: the technical survey for the fuel cell research network PACO. November 15, 2004 update no. 28; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 15 novembre 2004, no. 28

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    Summaries of several recent articles are gathered here. They deal with fuel cells, hydrogen production and storage. Their different titles are given below: 1)commercialization of fuel cells: technical and economical difficulties to the development of a hydrogen basic equipment 2)public directives to promote the acceptance of fuel cells for automotive industry 3)direct alcohol SOFC: current - voltage characteristics and composition of the fuel gas 4)control by the fuzzy logic method of an autonomous or connected to the electric system SOFC 5)determination of the energetic yield of a DMFC by a fuel circulation system 6)an hybrid system SOFC-Stirling motor of 5 kW 7)control system of PEM type APU feed with gasoline 8)development of a DMFC generator of 2W 8)analysis of the life cycle of the hydrogen production processes 9)hydrogen production for fuel cells by ethanol catalytic steam reforming 10)methanol production from coal: what are the aims of the research? 11)application of a membrane electrochemical reactor to the hydrogen production process by iodine-sulfur thermochemical cycles 12)hydrogen storage in carbonated nano-materials: possibilities and challenges. The references of these articles are detailed. (O.M.)

  18. Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy

    Science.gov (United States)

    Kemper, Björn; Bauwens, Andreas; Vollmer, Angelika; Ketelhut, Steffi; Langehanenberg, Patrik; Müthing, Johannes; Karch, Helge; von Bally, Gert

    2010-05-01

    Digital holographic microscopy (DHM) enables quantitative multifocus phase contrast imaging for nondestructive technical inspection and live cell analysis. Time-lapse investigations on human brain microvascular endothelial cells demonstrate the use of DHM for label-free dynamic quantitative monitoring of cell division of mother cells into daughter cells. Cytokinetic DHM analysis provides future applications in toxicology and cancer research.

  19. A stochastic frontier analysis of technical efficiency in smallholder maize production in Zimbabwe: The post-fast-track land reform outlook

    Directory of Open Access Journals (Sweden)

    Nelson Mango

    2015-12-01

    Full Text Available This article analyses the technical efficiency of maize production in Zimbabwe’s smallholder farming communities following the fast-track land reform of the year 2000 with a view of highlighting key entry points for policy. Using a randomly selected sample of 522 smallholder maize producers, a stochastic frontier production model was applied, using a linearised Cobb–Douglas production function to determine the production elasticity coefficients of inputs, technical efficiency and the determinants of efficiency. The study finds that maize output responds positively to increases in inorganic fertilisers, seed quantity, the use of labour and the area planted. The technical efficiency analysis suggests that about 90% of farmers in the sample are between 60 and 75% efficient, with an average efficiency in the sample of 65%. The significant determinants of technical efficiency were the gender of the household head, household size, frequency of extension visits, farm size and the farming region. The results imply that the average efficiency of maize production could be improved by 35% through better use of existing resources and technology. The results highlight the need for government and private sector assistance in improving efficiency by promoting access to productive resources and ensuring better and more reliable agricultural extension services.

  20. Large-scale differential display analysis of T helper cell differentiation.

    Science.gov (United States)

    Ojala, Pekka; Virtanen, Eveliina; Chen, Zhi

    2007-03-01

    We have developed a novel large-scale multicapillary fluorescent differential display (FDD) platform amenable to further automation. The power of the method is demonstrated by the analysis of T helper cell differentiation. Eight RNA samples from wild type, Stat4 knockout and Stat6 knockout mice were analyzed with 16 anchoring primers and 24 arbitrary primers, resulting in 285 294 sample peaks. Visually selected patterns of differential expression suggest two major regulatory mechanisms: activation and Stat4 genotype. A subset of the findings is reproduced in the confirmatory differential display (DD) that included technical and biological replicates. In a small fragment identification pilot study, we identify Ifi27 and Cct8 to be up-regulated by T cell activation. We present a method for the analysis of electropherogram similarity across large datasets, based on correlation of low-resolution representations of electrophoretic data. We show how it can be applied to analyze experimental and technical variables. Using this method, we demonstrate the effect of activation and genotype. In addition, agreement of our real experimental data to the theoretical basis of DD, as well as issues in anchoring primer selectivity, are studied.

  1. The logic of Technical Standardisation

    DEFF Research Database (Denmark)

    Nedergaard, Peter

    In this paper technical standardisation is understood and explained in a model where economic analysis is coupled with an analysis of the political system as proposed in rational choice theory. The aim is to answer both the question why various countries (e.g. the United States versus European...... countries) let either the market or public intervention determine the mode of technical standardisation and the possible implications of these two ways of organizing technical standardisation from an economic and a political point of view. Based upon the analysis of the paper a couple of general policy...

  2. Multispectral fingerprinting for improved in vivo cell dynamics analysis

    Directory of Open Access Journals (Sweden)

    Cooper Cameron HJ

    2010-09-01

    Full Text Available Abstract Background Tracing cell dynamics in the embryo becomes tremendously difficult when cell trajectories cross in space and time and tissue density obscure individual cell borders. Here, we used the chick neural crest (NC as a model to test multicolor cell labeling and multispectral confocal imaging strategies to overcome these roadblocks. Results We found that multicolor nuclear cell labeling and multispectral imaging led to improved resolution of in vivo NC cell identification by providing a unique spectral identity for each cell. NC cell spectral identity allowed for more accurate cell tracking and was consistent during short term time-lapse imaging sessions. Computer model simulations predicted significantly better object counting for increasing cell densities in 3-color compared to 1-color nuclear cell labeling. To better resolve cell contacts, we show that a combination of 2-color membrane and 1-color nuclear cell labeling dramatically improved the semi-automated analysis of NC cell interactions, yet preserved the ability to track cell movements. We also found channel versus lambda scanning of multicolor labeled embryos significantly reduced the time and effort of image acquisition and analysis of large 3D volume data sets. Conclusions Our results reveal that multicolor cell labeling and multispectral imaging provide a cellular fingerprint that may uniquely determine a cell's position within the embryo. Together, these methods offer a spectral toolbox to resolve in vivo cell dynamics in unprecedented detail.

  3. Large size biogas-fed Solid Oxide Fuel Cell power plants with carbon dioxide management: Technical and economic optimization

    Science.gov (United States)

    Curletti, F.; Gandiglio, M.; Lanzini, A.; Santarelli, M.; Maréchal, F.

    2015-10-01

    This article investigates the techno-economic performance of large integrated biogas Solid Oxide Fuel Cell (SOFC) power plants. Both atmospheric and pressurized operation is analysed with CO2 vented or captured. The SOFC module produces a constant electrical power of 1 MWe. Sensitivity analysis and multi-objective optimization are the mathematical tools used to investigate the effects of Fuel Utilization (FU), SOFC operating temperature and pressure on the plant energy and economic performances. FU is the design variable that most affects the plant performance. Pressurized SOFC with hybridization with a gas turbine provides a notable boost in electrical efficiency. For most of the proposed plant configurations, the electrical efficiency ranges in the interval 50-62% (LHV biogas) when a trade-off of between energy and economic performances is applied based on Pareto charts obtained from multi-objective plant optimization. The hybrid SOFC is potentially able to reach an efficiency above 70% when FU is 90%. Carbon capture entails a penalty of more 10 percentage points in pressurized configurations mainly due to the extra energy burdens of captured CO2 pressurization and oxygen production and for the separate and different handling of the anode and cathode exhausts and power recovery from them.

  4. TECHNICAL COORDINATION

    CERN Multimedia

    A. Ball

    Overview From a technical perspective, CMS has been in “beam operation” state since 6th November. The detector is fully closed with all components operational and the magnetic field is normally at the nominal 3.8T. The UXC cavern is normally closed with the radiation veto set. Access to UXC is now only possible during downtimes of LHC. Such accesses must be carefully planned, documented and carried out in agreement with CMS Technical Coordination, Experimental Area Management, LHC programme coordination and the CCC. Material flow in and out of UXC is now strictly controlled. Access to USC remains possible at any time, although, for safety reasons, it is necessary to register with the shift crew in the control room before going down.It is obligatory for all material leaving UXC to pass through the underground buffer zone for RP scanning, database entry and appropriate labeling for traceability. Technical coordination (notably Stephane Bally and Christoph Schaefer), the shift crew and run ...

  5. TECHNICAL COORDINATION

    CERN Multimedia

    A. Ball and W. Zeuner

    2011-01-01

    In this report we will review the main achievements of the Technical Stop and the progress of several centrally-managed projects to support CMS operation and maintenance and prepare the way for upgrades. Overview of the extended Technical Stop  The principal objectives of the extended Technical Stop affecting the detector itself were the installation of the TOTEM T1 telescopes on both ends, the readjustment of the alignment link-disk in YE-2, the replacement of the light-guide sleeves for all PMs of both HFs, and some repairs on TOTEM T2 and CASTOR. The most significant tasks were, however, concentrated on the supporting infrastructure. A detailed line-by-line leak search was performed in the C6F14 cooling system of the Tracker, followed by the installation of variable-frequency drives on the pump motors of the SS1 and SS2 tracker cooling plants to reduce pressure transients during start-up. In the electrical system, larger harmonic filters were installed in ...

  6. Comparative analysis of dynamic cell viability, migration and invasion assessments by novel real-time technology and classic endpoint assays.

    Directory of Open Access Journals (Sweden)

    Ridha Limame

    Full Text Available BACKGROUND: Cell viability and motility comprise ubiquitous mechanisms involved in a variety of (pathobiological processes including cancer. We report a technical comparative analysis of the novel impedance-based xCELLigence Real-Time Cell Analysis detection platform, with conventional label-based endpoint methods, hereby indicating performance characteristics and correlating dynamic observations of cell proliferation, cytotoxicity, migration and invasion on cancer cells in highly standardized experimental conditions. METHODOLOGY/PRINCIPAL FINDINGS: Dynamic high-resolution assessments of proliferation, cytotoxicity and migration were performed using xCELLigence technology on the MDA-MB-231 (breast cancer and A549 (lung cancer cell lines. Proliferation kinetics were compared with the Sulforhodamine B (SRB assay in a series of four cell concentrations, yielding fair to good correlations (Spearman's Rho 0.688 to 0.964. Cytotoxic action by paclitaxel (0-100 nM correlated well with SRB (Rho>0.95 with similar IC(50 values. Reference cell migration experiments were performed using Transwell plates and correlated by pixel area calculation of crystal violet-stained membranes (Rho 0.90 and optical density (OD measurement of extracted dye (Rho>0.95. Invasion was observed on MDA-MB-231 cells alone using Matrigel-coated Transwells as standard reference method and correlated by OD reading for two Matrigel densities (Rho>0.95. Variance component analysis revealed increased variances associated with impedance-based detection of migration and invasion, potentially caused by the sensitive nature of this method. CONCLUSIONS/SIGNIFICANCE: The xCELLigence RTCA technology provides an accurate platform for non-invasive detection of cell viability and motility. The strong correlations with conventional methods imply a similar observation of cell behavior and interchangeability with other systems, illustrated by the highly correlating kinetic invasion profiles on

  7. Digital Microfluidics for Manipulation and Analysis of a Single Cell

    Directory of Open Access Journals (Sweden)

    Jie-Long He

    2015-09-01

    Full Text Available The basic structural and functional unit of a living organism is a single cell. To understand the variability and to improve the biomedical requirement of a single cell, its analysis has become a key technique in biological and biomedical research. With a physical boundary of microchannels and microstructures, single cells are efficiently captured and analyzed, whereas electric forces sort and position single cells. Various microfluidic techniques have been exploited to manipulate single cells through hydrodynamic and electric forces. Digital microfluidics (DMF, the manipulation of individual droplets holding minute reagents and cells of interest by electric forces, has received more attention recently. Because of ease of fabrication, compactness and prospective automation, DMF has become a powerful approach for biological application. We review recent developments of various microfluidic chips for analysis of a single cell and for efficient genetic screening. In addition, perspectives to develop analysis of single cells based on DMF and emerging functionality with high throughput are discussed.

  8. Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Maxwell, Mike, J., P.E.

    2012-08-30

    The STI product is the Final Technical Report from ReliOn, Inc. for contract award DE-EE0000487: Recovery Act PEM Fuel Cell Systems Providing Emergency Reserve and Backup Power. The program covered the turnkey deployment of 431 ReliOn fuel cell systems at 189 individual sites for AT&T and PG&E with ReliOn functioning as the primary equipment supplier and the project manager. The Final Technical Report provides an executive level summary, a comparison of the actual accomplishments vs. the goals and objectives of the project, as well as a summary of the project activity from the contract award date of August 1, 2009 through the contract expiration date of December 31, 2011. Two photos are included in the body of the report which show hydrogen storage and bulk hydrogen refueling technologies developed as a result of this program.

  9. mRNA analysis of single living cells

    Directory of Open Access Journals (Sweden)

    Ikai Atsushi

    2003-02-01

    Full Text Available Abstract Analysis of specific gene expression in single living cells may become an important technique for cell biology. So far, no method has been available to detect mRNA in living cells without killing or destroying them. We have developed here a novel method to examine gene expression of living cells using an atomic force microscope (AFM. AFM tip was inserted into living cells to extract mRNAs. The obtained mRNAs were analyzed with RT-PCR, nested PCR, and quantitative PCR. This method enabled us to examine time-dependent gene expression of single living cells without serious damage to the cells.

  10. Modeling Technical Change in Energy System Analysis: Analyzing the Introduction of Learning-by-Doing in Bottom-up Energy Models

    Energy Technology Data Exchange (ETDEWEB)

    Berglund, Christer; Soederholm, Patrik [Luleaa Univ. of Technology (Sweden). Div. of Economics

    2005-02-01

    The main objective of this paper is to provide an overview and a critical analysis of the recent literature on incorporating induced technical change in energy systems models. Special emphasis is put on surveying recent studies aiming at integrating learning-by-doing into bottom-up energy systems models through so-called learning curves, and on analyzing the relevance of learning curve analysis for understanding the process of innovation and technology diffusion in the energy sector. The survey indicates that this model work represents a major advance in energy research, and embeds important policy implications, not the least concerning the cost and the timing of environmental policies (including carbon emission constraints). However, bottom-up energy models with endogenous learning are also limited in their characterization of technology diffusion and innovation. While they provide a detailed account of technical options - which is absent in many top-down models - they also lack important aspects of diffusion behavior that are captured in top-down representations. For instance, they fail in capturing strategic technology diffusion behavior in the energy sector, and they neglect important general equilibrium impacts (such as the opportunity cost of redirecting R and D support to the energy sector). For these reasons bottom-up and top-down models with induced technical change should not be viewed as substitutes but rather as complements.

  11. Single-cell analysis in cancer genomics

    Science.gov (United States)

    Saadatpour, Assieh; Lai, Shujing; Guo, Guoji; Yuan, Guo-Cheng

    2017-01-01

    Genetic changes and environmental differences result in cellular heterogeneity among cancer cells within the same tumor, thereby complicating treatment outcomes. Recent advances in single-cell technologies have opened new avenues to characterize the intra-tumor cellular heterogeneity, identify rare cell types, measure mutation rates, and, ultimately, guide diagnosis and treatment. In this paper, we review the recent single-cell technological and computational advances at the genomic, transcriptomic, and proteomic levels, and discuss their applications in cancer research. PMID:26450340

  12. Analysis of Teachers' Adoption of Technology for Use in Instruction in Seven Career and Technical Education Programs

    Science.gov (United States)

    Kotrlik, Joe W.; Redmann, Donna H.

    2009-01-01

    This study addressed utilization of technology in instruction by secondary career and technical education (CTE) teachers in seven program areas in Louisiana. A stratified random sample was utilized, with 539 teachers responding to the survey after three data collection efforts. The data were determined to be representative of all CTE teachers in…

  13. Syntactical and Punctuation Errors: An Analysis of Technical Writing of University Students Science College, Taif University, KSA

    Science.gov (United States)

    Alamin, Abdulamir; Ahmed, Sawsan

    2012-01-01

    Analyzing errors committed by second language learners during their first year of study at the University of Taif, can offer insights and knowledge of the learners' difficulties in acquiring technical English communication. With reference to the errors analyzed, the researcher found that the learners' failure to understand basic English grammar…

  14. Flood Protection in Venice under Conditions of Sea-Level Rise: An Analysis of Institutional and Technical Measures

    NARCIS (Netherlands)

    Munaretto, S.; Vellinga, P.; Tobi, H.

    2012-01-01

    It is widely acknowledged that in times of climate change loss of coastal resources and risk for human life can be minimized by implementing adaptation strategies. Such strategies need to encompass a balanced mix of non-structural (institutional) and structural (technical) measures based on sound sc

  15. Technical-economic assessment of the production of methanol from biomass. Conversion process analysis. Final research report

    Energy Technology Data Exchange (ETDEWEB)

    Wan, E.I.; Simmons, J.A.; Price, J.D.; Nguyen, T.D.

    1979-07-12

    A comprehensive engineering system study was conducted to assess various thermochemical processes suitable for converting biomass to methanol. A summary of the conversion process study results is presented here, delineating the technical and economic feasibilities of producing methanol fuel from biomass utilizing the currently available technologies. (MHR)

  16. Analysis of survival and morbidity after pediatric liver transplantation with full-size and technical-variant grafts

    NARCIS (Netherlands)

    Sieders, E; Peeters, PMJG; TenVergert, EM; Bijleveld, CMA; De Jong, KP; Zwaveling, JH; Boersma, GA; Slooff, MJH

    1999-01-01

    Background To alleviate the shortage of size-matched whole-donor organs, too-large-for-size cadaveric donor grafts are modified by liver resection techniques. These modifications result in technical-variant liver transplantation (TVLTx). Patient and graft survival rates after TVLTx are considered co

  17. The Effects of Vocational Training on Labor Force Experience. An Analysis of the Tennessee Area Vocational Technical School System.

    Science.gov (United States)

    Schriver, William R.; Bowlby, Roger L.

    To analyze the differential benefits from vocational training, a study was made involving 1,701 former students selected at random from 19 area vocational-technical schools in Tennessee. Major objectives of the study were to determine: (1) economic justification of vocational training, (2) wage differences among the vocationally trained and…

  18. Control and analysis of dynamics of technical and tactical actions in defence during the game in basketball players of superleague team

    Directory of Open Access Journals (Sweden)

    Olena Mitova

    2015-06-01

    Full Text Available Purpose: to analyze the dynamics of technical and tactical actions in defense during the match in basketball major league team. Material and Methods: the methods were applied to analyze and summarize the literature, analysis of statistical reports, teacher observation of competitive activity, methods of mathematical statistics. Results: by monitoring and analyzed dynamics of technical and tactical actions in the defense during a game of basketball at the major league team. Conclusions: basketball players show the highest indicators in the third quarter and at the end of the match – in the fourth quarter there is a gradual decrease in indicators such as: the rebounds, block shots, fouls and steels, and the increase – missing the shots, ball loss.

  19. Fuel cells principles, design, and analysis

    CERN Document Server

    Revankar, Shripad T

    2014-01-01

    ""This book covers all essential themes of fuel cells ranging from fundamentals to applications. It includes key advanced topics important for understanding correctly the underlying multi-science phenomena of fuel cell processes. The book does not only cope with traditional fuel cells but also discusses the future concepts of fuel cells. The book is rich on examples and solutions important for applying the theory into practical use.""-Peter Lund, Aalto University, Helsinki""A good introduction to the range of disciplines needed to design, build and test fuel cells.""-Nigel Brandon, Imperial Co

  20. Droplet microfluidics--a tool for single-cell analysis.

    Science.gov (United States)

    Joensson, Haakan N; Andersson Svahn, Helene

    2012-12-03

    Droplet microfluidics allows the isolation of single cells and reagents in monodisperse picoliter liquid capsules and manipulations at a throughput of thousands of droplets per second. These qualities allow many of the challenges in single-cell analysis to be overcome. Monodispersity enables quantitative control of solute concentrations, while encapsulation in droplets provides an isolated compartment for the single cell and its immediate environment. The high throughput allows the processing and analysis of the tens of thousands to millions of cells that must be analyzed to accurately describe a heterogeneous cell population so as to find rare cell types or access sufficient biological space to find hits in a directed evolution experiment. The low volumes of the droplets make very large screens economically viable. This Review gives an overview of the current state of single-cell analysis involving droplet microfluidics and offers examples where droplet microfluidics can further biological understanding.

  1. Analysis of mitochondria isolated from single cells.

    Science.gov (United States)

    Johnson, Ryan D; Navratil, Marian; Poe, Bobby G; Xiong, Guohua; Olson, Karen J; Ahmadzadeh, Hossein; Andreyev, Dmitry; Duffy, Ciarán F; Arriaga, Edgar A

    2007-01-01

    Bulk studies are not suitable to describe and study cell-to-cell variation, which is of high importance in biological processes such as embryogenesis, tissue differentiation, and disease. Previously, capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) was used to measure the properties of organelles isolated from millions of cells. As such, these bulk measurements reported average properties for the organelles of cell populations. Similar measurements for organelles released from single cells would be highly relevant to describe the subcellular variations among cells. Toward this goal, here we introduce an approach to analyze the mitochondria released from single mammalian cells. Osteosarcoma 143B cells are labeled with either the fluorescent mitochondrion-specific 10-N-nonyl acridine orange (NAO) or via expression of the fluorescent protein DsRed2. Subsequently, a single cell is introduced into the CE-LIF capillary where the organelles are released by a combined treatment of digitonin and trypsin. After this treatment, an electric field is applied and the released organelles electromigrate toward the LIF detector. From an electropherogram, the number of detected events per cell, their individual electrophoretic mobilities, and their individual fluorescence intensities are calculated. The results obtained from DsRed2 labeling, which is retained in intact mitochondria, and NAO labeling, which labels all mitochondria, are the basis for discussion of the strengths and limitations of this single-cell approach.

  2. Wide band gap solar cells with high stabilized performance. Annual technical report, 15 July 1995--15 July 1996

    Energy Technology Data Exchange (ETDEWEB)

    Wronski, C R; Collins, R W; Fujiwara, H [Pennsylvania State Univ., University Park, PA (United States); and others

    1997-01-01

    This report describes work on an improved understanding of stability in materials and silicon solar cells. Topics include novel intrinsic materials optimization; solar cells optimized for p- and i-layer performance; novel p-type materials; interfaces; and device modeling.

  3. TECHNICAL COORDINATION

    CERN Multimedia

    A. Ball and W. Zeuner

    2011-01-01

      Operational experience 2011 CMS is approaching the end of a very successful year of operation. Proton- proton running ended in the late afternoon of 30th October with a stunning 5.73 fb–1 delivered by LHC, of which CMS recorded 5.22 fb–1. During heavy-ion operation, which continues until 7th December, both the accelerator and the CMS detector have also performed very well. Despite the encouraging overall reliability of technical operation, several infrastructure failures which occurred since the last Bulletin are worthy of mention, with one leading for the first time to significant data-loss. On 10th July, a CERN-wide power failure brought down essentially all services including the magnet, due to an MCS setting being left in “manual” after the recent technical stop, but there was no significant damage and the detector was operational before the LHC, despite a slow and tortuous recovery (one of several indications this year that there is room for improve...

  4. TECHNICAL COORDINATION

    CERN Multimedia

    A. Ball and W. Zeuner

    2013-01-01

    For the reporting period, the CMS common systems and infrastructure worked well, without failures that caused significant data losses. One more disconnection of the magnet cold box occurred in the shadow of interruptions in data taking, caused by a series of technical faults. The recognition during 2012 that re-connection can only safely be done at around 2 T implies a minimum magnet recovery time of 12 hours and raises serious concerns about the number of ramping cycles of the magnet these incidents cause. This has triggered studies of how to make the cryo-system of the magnet more robust against failures. The proton-proton run ended just before the end-of-year CERN closure, during which CASTOR was installed on the negative end of CMS and both ZDC calorimeters were installed in TAN absorbers the LHC tunnel, in preparation for the heavy-ion run. The installation of CASTOR was an excellent “engineering test” of procedures for working in an activated environment. Despite some technical pr...

  5. TECHNICAL COORDINATION

    CERN Multimedia

    Austin Ball

    Summary of progress since last CMS week. Ten years of construction work have been completed. CMS is closed, in very close to the ideal low luminosity configuration, and performed well in the first tests with LHC beam. Behind this encouraging news is the story of a summer of intense commitment by many teams (from the collaboration and 3 CERN departments) working together, against the clock and despite many minor setbacks, to ensure that the experiment was ready to play a leading role in the excitement of September 10. Following beampipe bakeout and refill with pure neon, a magnificent effort by the ECAL group and the pt 5 technical crew made it possible to install and commission all 4 ECAL endcap Dees before the end of August. In the shadow of this activity, the barrel and forward pixel trackers and part of the beam monitoring were installed within the vac tank. The pt 5 technical teams then succeeded in safely removing the 20t installation tables and their support blocks from beneath the already installed ...

  6. Technical support document: Energy conservation standards for consumer products: Dishwashers, clothes washers, and clothes dryers including: Environmental impacts; regulatory impact analysis

    Energy Technology Data Exchange (ETDEWEB)

    1990-12-01

    The Energy Policy and Conservation Act as amended (P.L. 94-163), establishes energy conservation standards for 12 of the 13 types of consumer products specifically covered by the Act. The legislation requires the Department of Energy (DOE) to consider new or amended standards for these and other types of products at specified times. This Technical Support Document presents the methodology, data and results from the analysis of the energy and economic impacts of standards on dishwashers, clothes washers, and clothes dryers. The economic impact analysis is performed in five major areas: An Engineering Analysis, which establishes technical feasibility and product attributes including costs of design options to improve appliance efficiency. A Consumer Analysis at two levels: national aggregate impacts, and impacts on individuals. The national aggregate impacts include forecasts of appliance sales, efficiencies, energy use, and consumer expenditures. The individual impacts are analyzed by Life-Cycle Cost (LCC), Payback Periods, and Cost of Conserved Energy (CCE), which evaluate the savings in operating expenses relative to increases in purchase price; A Manufacturer Analysis, which provides an estimate of manufacturers' response to the proposed standards. Their response is quantified by changes in several measures of financial performance for a firm. An Industry Impact Analysis shows financial and competitive impacts on the appliance industry. A Utility Analysis that measures the impacts of the altered energy-consumption patterns on electric utilities. A Environmental Effects analysis, which estimates changes in emissions of carbon dioxide, sulfur oxides, and nitrogen oxides, due to reduced energy consumption in the home and at the power plant. A Regulatory Impact Analysis collects the results of all the analyses into the net benefits and costs from a national perspective. 47 figs., 171 tabs. (JF)

  7. Statistical Survival Analysis of Fish and Wildlife Tagging Studies; SURPH.1 Manual - Analysis of Release-Recapture Data for Survival Studies, 1994 Technical Manual.

    Energy Technology Data Exchange (ETDEWEB)

    Smith, Steven G.; Skalski, John R.; Schelechte, J. Warren [Univ. of Washington, Seattle, WA (United States). Center for Quantitative Science

    1994-12-01

    Program SURPH is the culmination of several years of research to develop a comprehensive computer program to analyze survival studies of fish and wildlife populations. Development of this software was motivated by the advent of the PIT-tag (Passive Integrated Transponder) technology that permits the detection of salmonid smolt as they pass through hydroelectric facilities on the Snake and Columbia Rivers in the Pacific Northwest. Repeated detections of individually tagged smolt and analysis of their capture-histories permits estimates of downriver survival probabilities. Eventual installation of detection facilities at adult fish ladders will also permit estimation of ocean survival and upstream survival of returning salmon using the statistical methods incorporated in SURPH.1. However, the utility of SURPH.1 far exceeds solely the analysis of salmonid tagging studies. Release-recapture and radiotelemetry studies from a wide range of terrestrial and aquatic species have been analyzed using SURPH.1 to estimate discrete time survival probabilities and investigate survival relationships. The interactive computing environment of SURPH.1 was specifically developed to allow researchers to investigate the relationship between survival and capture processes and environmental, experimental and individual-based covariates. Program SURPH.1 represents a significant advancement in the ability of ecologists to investigate the interplay between morphologic, genetic, environmental and anthropogenic factors on the survival of wild species. It is hoped that this better understanding of risk factors affecting survival will lead to greater appreciation of the intricacies of nature and to improvements in the management of wild resources. This technical report is an introduction to SURPH.1 and provides a user guide for both the UNIX and MS-Windows{reg_sign} applications of the SURPH software.

  8. Technical Review Report for the Safety Analysis Report for Packaging Model 9977 S-SARP-G-00001 Revision 2

    Energy Technology Data Exchange (ETDEWEB)

    DiSabatino, A; Hafner, R; West, M

    2007-10-04

    This Technical Review Report (TRR) summarizes the review findings for the Safety Analysis Report for Packaging (SARP) for the Model 9977 B(M)F-96 shipping container. The content analyzed for this submittal is Content Envelope C.1, Heat Sources, in assemblies of Radioisotope Thermoelectric Generators or food-pack cans. The SARP under review, i.e., S-SARP-G-00001, Revision 2 (August 2007), was originally referred to as the General Purpose Fissile Material Package. The review presented in this TRR was performed using the methods outlined in Revision 3 of the Department of Energy's (DOE's) Packaging Review Guide (PRG) for Reviewing Safety Analysis Reports for Packages. The format of the SARP follows that specified in Revision 2 of the Nuclear Regulatory Commission's, Regulatory Guide 7.9, i.e., Standard Format and Content of Part 71 Applications for Approval of Packages for Radioactive Material. Although the two documents are similar in their content, they are not identical. Formatting differences have been noted in this TRR, where appropriate. The Model 9977 Package is a 35-gallon drum package design that has evolved from a family of packages designed by DOE contractors at the Savannah River Site. The Model 9977 Package design includes a single, 6-inch diameter, stainless steel pressure vessel containment system (i.e., the 6CV) that was designed and fabricated in accordance with Section III, Subsection NB, of the American Society of Mechanical Engineers Boiler & Pressure Vessel Code. The earlier package designs, i.e., the Model 9965, 9966, 9967 and 9968 Packages, were originally designed and certified in the 1980s. In the 1990s, updated package designs that incorporated design features consistent with new safety requirements, based on International Atomic Energy Agency guidelines, were proposed. The updated package designs were the Model 9972, 9973, 9974 and 9975 Packages, respectively. The Model 9975 Package was certified by the Packaging Certification

  9. Single-cell transcriptome analysis of endometrial tissue

    OpenAIRE

    Krjutškov, K.; Katayama, S .; Saare, M; Vera-Rodriguez, M.; Lubenets, D.; Samuel, K.; Laisk-Podar, T.; Teder, H.; Einarsdottir, E.; Salumets, A.; Kere, J.

    2016-01-01

    STUDY QUESTION How can we study the full transcriptome of endometrial stromal and epithelial cells at the single-cell level? SUMMARY ANSWER By compiling and developing novel analytical tools for biopsy, tissue cryopreservation and disaggregation, single-cell sorting, library preparation, RNA sequencing (RNA-seq) and statistical data analysis. WHAT IS KNOWN ALREADY Although single-cell transcriptome analyses from various biopsied tissues have been published recently, corresponding protocols fo...

  10. Electrochemical nanoprobes for single-cell analysis.

    Science.gov (United States)

    Actis, Paolo; Tokar, Sergiy; Clausmeyer, Jan; Babakinejad, Babak; Mikhaleva, Sofya; Cornut, Renaud; Takahashi, Yasufumi; López Córdoba, Ainara; Novak, Pavel; Shevchuck, Andrew I; Dougan, Jennifer A; Kazarian, Sergei G; Gorelkin, Petr V; Erofeev, Alexander S; Yaminsky, Igor V; Unwin, Patrick R; Schuhmann, Wolfgang; Klenerman, David; Rusakov, Dmitri A; Sviderskaya, Elena V; Korchev, Yuri E

    2014-01-28

    The measurement of key molecules in individual cells with minimal disruption to the biological milieu is the next frontier in single-cell analyses. Nanoscale devices are ideal analytical tools because of their small size and their potential for high spatial and temporal resolution recordings. Here, we report the fabrication of disk-shaped carbon nanoelectrodes whose radius can be precisely tuned within the range 5-200 nm. The functionalization of the nanoelectrode with platinum allowed the monitoring of oxygen consumption outside and inside a brain slice. Furthermore, we show that nanoelectrodes of this type can be used to impale individual cells to perform electrochemical measurements within the cell with minimal disruption to cell function. These nanoelectrodes can be fabricated combined with scanning ion conductance microscopy probes, which should allow high resolution electrochemical mapping of species on or in living cells.

  11. Sensitivity Analysis of Centralized Dynamic Cell Selection

    DEFF Research Database (Denmark)

    Lopez, Victor Fernandez; Alvarez, Beatriz Soret; Pedersen, Klaus I.;

    2016-01-01

    mechanism and solutions involving cell switching in general. Simulation results show that such solutions can greatly benefit from the use of receivers with interference suppression capabilities and a larger number of antennas, with a maximum data rate gain of 120%. High performance gains are observed...... with two different traffic models, and it is not necessary to be able to connect to a large number of cells in order to reap most of the benefits of the centralized dynamic cell selection....

  12. The influence of biological and technical factors on quantitative analysis of amyloid PET: Points to consider and recommendations for controlling variability in longitudinal data.

    Science.gov (United States)

    Schmidt, Mark E; Chiao, Ping; Klein, Gregory; Matthews, Dawn; Thurfjell, Lennart; Cole, Patricia E; Margolin, Richard; Landau, Susan; Foster, Norman L; Mason, N Scott; De Santi, Susan; Suhy, Joyce; Koeppe, Robert A; Jagust, William

    2015-09-01

    In vivo imaging of amyloid burden with positron emission tomography (PET) provides a means for studying the pathophysiology of Alzheimer's and related diseases. Measurement of subtle changes in amyloid burden requires quantitative analysis of image data. Reliable quantitative analysis of amyloid PET scans acquired at multiple sites and over time requires rigorous standardization of acquisition protocols, subject management, tracer administration, image quality control, and image processing and analysis methods. We review critical points in the acquisition and analysis of amyloid PET, identify ways in which technical factors can contribute to measurement variability, and suggest methods for mitigating these sources of noise. Improved quantitative accuracy could reduce the sample size necessary to detect intervention effects when amyloid PET is used as a treatment end point and allow more reliable interpretation of change in amyloid burden and its relationship to clinical course.

  13. New Metallization Technique Suitable for 6-MW Pilot Production of Efficient Multicrystalline Solar Cells Using Upgraded Metallurgical Silicon: Final Technical Progress Report, December 17, 2007 -- June 16, 2009

    Energy Technology Data Exchange (ETDEWEB)

    Ounadjela, K.; Blosse, A.

    2010-08-01

    This report describes CaliSolar's work as a Photovoltaic Technology Incubator awardee within the U.S. Department of Energy's Solar Energy Technologies Program. The term of this subcontract with the National Renewable Energy Laboratory was two years. During this time, CaliSolar evolved from a handful of employees to over 100 scientists, engineers, technicians, and operators. On the technical side, the company transitioned from a proof-of-concept through pilot-scale to large-scale industrial production. A fully automated 60-megawatt manufacturing line was commissioned in Sunnyvale, California. The facility converts upgraded metallurgical-grade silicon feedstock to ingots, wafers, and high-efficiency multicrystalline solar cells.

  14. A modified release analysis procedure using advanced froth flotation mechanisms. Technical report, September 1--November 30, 1995

    Energy Technology Data Exchange (ETDEWEB)

    Honaker, R.Q.; Mohanty, M.K. [Southern Illinois Univ., Carbondale, IL (United States). Dept. of Mining Engineering

    1995-12-31

    The objective of this study is to reinvestigate the release analysis procedure, which is traditionally conducted using a laboratory Denver cell, and to develop a modified process that can be used for all froth flotation technologies. Recent studies have found that the separation performance achieved by multiple stage cleaning and, in some cases, single stage cleaning using column flotation is superior to the performance achieved by the traditional release procedure. These findings are a result of the advanced flotation mechanisms provided by column flotation, which will be incorporated into a modified release analysis procedure developed in this study. A fundamental model of an open column has been developed which incorporates the effects of system hydrodynamics, froth drop-back, selective and non-selective detachment, operating parameters, feed solids content, and feed component flotation kinetics. Simulation results obtained during this reporting period indicate that the ultimate separation that can be achieved by a column flotation process can only be obtained in a single cleaning stage if the detachment mechanism in the froth phase is highly selective, which does not appear to occur in practice based on experimental results. Two to three cleaning stages were found to be required to obtain the ultimate performance if non-selective detachment or kinetic limiting conditions are assumed. this simulated finding agrees well with the experimental results obtained from the multiple stage cleaning of an Illinois No. 5 seam coal using the Packed-Column. Simulated results also indicate that the separation performance achieved by column flotation improves with increasing feed solids content after carrying-capacity limiting conditions are realized. These findings will be utilized in the next reporting period to modify the traditional release analysis procedure.

  15. Process Development for High Voc CdTe Solar Cells: Phase I, Annual Technical Report, October 2005 - September 2006

    Energy Technology Data Exchange (ETDEWEB)

    Ferekides, C. S.; Morel, D. L.

    2007-04-01

    The focus of this project is the open-circuit voltage of the CdTe thin-film solar cell. CdTe continues to be one of the leading materials for large-scale cost-effective production of photovoltaics, but the efficiency of the CdTe solar cell has been stagnant for the last few years. At the manufacturing front, the CdTe technology is fast paced and moving forward with U.S.-based First Solar LLC leading the world in CdTe module production. To support the industry efforts and continue the advancement of this technology, it will be necessary to continue improvements in solar cell efficiency. A closer look at the state-of-the-art performance levels puts the three solar cell efficiency parameters of short-circuit current density (JSC), open-circuit voltage (VOC), and fill factor (FF) in the 24-26 mA/cm2, 844?850 mV, and 74%-76% ranges respectively. During the late 1090s, efforts to improve cell efficiency were primarily concerned with increasing JSC, simply by using thinner CdS window layers to enhance the blue response (<510 nm) of the CdTe cell. These efforts led to underscoring the important role 'buffers' (or high-resistivity transparent films) play in CdTe cells. The use of transparent bi-layers (low-p/high-p) as the front contact is becoming a 'standard' feature of the CdTe cell.

  16. Analysis of Source Selection Methods and Performance Outcomes: Lowest Price Technically Acceptable vs. Tradeoff in Air Force Acquisitions

    Science.gov (United States)

    2015-12-01

    agencies, the U.S. Department of State is forbidden by law to select anything but the lowest price and ‘technically acceptable ’ offer when awarding...factors are based on an “ acceptable ” or “unacceptable” basis, the award is simply made to the bidder offering the lowest price with an acceptable ... acceptable level of performance. With this information, industry will know what the competitive effect of offering higher performance will be and

  17. Normalized polarization ratios for the analysis of cell polarity.

    Directory of Open Access Journals (Sweden)

    Raz Shimoni

    Full Text Available The quantification and analysis of molecular localization in living cells is increasingly important for elucidating biological pathways, and new methods are rapidly emerging. The quantification of cell polarity has generated much interest recently, and ratiometric analysis of fluorescence microscopy images provides one means to quantify cell polarity. However, detection of fluorescence, and the ratiometric measurement, is likely to be sensitive to acquisition settings and image processing parameters. Using imaging of EGFP-expressing cells and computer simulations of variations in fluorescence ratios, we characterized the dependence of ratiometric measurements on processing parameters. This analysis showed that image settings alter polarization measurements; and that clustered localization is more susceptible to artifacts than homogeneous localization. To correct for such inconsistencies, we developed and validated a method for choosing the most appropriate analysis settings, and for incorporating internal controls to ensure fidelity of polarity measurements. This approach is applicable to testing polarity in all cells where the axis of polarity is known.

  18. Technical presentation

    CERN Multimedia

    FP Department

    2009-01-01

    07 April 2009 Technical presentation by Leuze Electronics: 14.00 – 15.00, Main Building, Room 61-1-017 (Room A) Photoelectric sensors, data identification and transmission systems, image processing systems. We at Leuze Electronics are "the sensor people": we have been specialising in optoelectronic sensors and safety technology for accident prevention for over 40 years. Our dedicated staff are all highly customer oriented. Customers of Leuze Electronics can always rely on one thing – on us! •\tFounded in 1963 •\t740 employees •\t115 MEUR turnover •\t20 subsidiaries •\t3 production facilities in southern Germany Product groups: •\tPhotoelectric sensors •\tIdentification and measurements •\tSafety devices

  19. Technical presentation

    CERN Multimedia

    FI Department

    2008-01-01

    RADIOSPARES, the leading catalogue distributor of components (electronic, electrical, automation, etc.) and industrial supplies will be at CERN on Friday 3 October 2008 (Main Building, Room B, from 9.00 a.m. to 3.00 p.m.) to introduce its new 2008/2009 catalogue. This will be the opportunity for us to present our complete range of products in more detail: 400 000 part numbers available on our web site (Radiospares France, RS International, extended range of components from other manufacturers); our new services: quotations, search for products not included in the catalogue, SBP products (Small Batch Production: packaging in quantities adapted to customers’ requirements); partnership with our focus manufacturers; demonstration of the on-line purchasing tool implemented on our web site in conjunction with CERN. RADIOSPARES will be accompanied by representatives of FLUKE and TYCO ELECTRONICS, who will make presentations, demonstrate materials and answer any technical questio...

  20. TECHNICAL COORDINATION

    CERN Multimedia

    A. Ball and W. Zeuner

    2010-01-01

    Overview Once again, the bulk of this article reviews the intense activity of a recently completed shutdown, which, although quite unforeseeable until a few weeks before it started, proved by its success that our often advertised capability to conduct major maintenance within a two month period is real. Although safely completed, on-time to remarkable precision, the activity was not without incident, and highlighted our dependence on many experienced, specialist teams and their precise choreography. Even after the yoke was safely closed, magnet re-commissioning and beampipe pumpdown showed new and thought-provoking behaviour. The struggle to maintain adequate technical resources will be a pre-occupation over the coming months, in parallel with the start of truly sustained operation, for which various procedures are still being put in place. Planning for future shutdowns must now become a high priority, with many working groups and task forces already in existence to prepare infrastructure improvements and to...

  1. Technical presentation

    CERN Multimedia

    GS Department

    2010-01-01

    10 March 2010 DYNEOS 10:00 – 12:00 - Main Building, Room B, 61-1-009 Dyneos AG is active in the fields of photonics, laser and high-precision positioning. Our highly qualified engineer team has more than 30 years of experience in electro-optical solutions sales. The engineers are supported by a technical and administrative team. We are focused on the Swiss market and represent six suppliers (Coherent, PI Physik Instrumente, SIOS, Nanonics Imaging, APE, Ekspla) in order to give a qualified sales and service support to our customers. Our products are dedicated to the research field as well as to industry. In addition to standard catalog products, we offer custom designs to fulfill the specific needs of OEM customers or specific applications.

  2. Flux analysis of mammalian cell culture

    NARCIS (Netherlands)

    Martens, D.E.; Tramper, J.

    2010-01-01

    Animal cells are used for the production of vaccines and pharmaceutical proteins. The increase in demand for these products requires an increase in volumetric productivity of animal cell culture processes, which can be attained through an increase in biomass concentration and/or specific productivit

  3. Technical Training: Technical Training Seminar

    CERN Multimedia

    2004-01-01

    TECHNICAL TRAINING Monique Duval tel. 74924 technical.training@cern.ch Tuesday 3 February 2004 From 09:00 to 13:30 - Training Centre Auditorium - bldg. 593, room 11 USB (Universal Serial Bus) CYPRESS Seminar Claudia Colombini, Field Application Engineer CYPRESS ActiveComp Electronic GmbH D-85077 MANCHING, Germany As a pioneer in USB, CYPRESS sets the standard for cost-effective solutions without sacrificing functionality, performance or reliability. Having shipped over 200 million USB devices, Cypress is the undisputed market leader and demonstrates unmatched USB expertise. With the industry's broadest selection of USB solutions, Cypress has the right silicon, software and support for every USB application, from Low-speed to High-Speed and USB On-The-Go (OTG). 9:00 - 10:30 Overview of USB systems. USB CYPRESS product overview. Peripherals: Low Speed, Full Speed, High Speed (1.1 and 2.0). Hub Solutions, Embedded Host Solutions, On-The-Go (OTG) and wireless USB. USB Development Tools (first part) 10:30 -...

  4. Micro and nano-platforms for biological cell analysis

    DEFF Research Database (Denmark)

    Svendsen, Winnie Edith; Castillo, Jaime; Moresco, Jacob Lange;

    2011-01-01

    In this paper some technological platforms developed for biological cell analysis will be presented and compared to existing systems. In brief, we present a novel micro cell culture chamber based on diffusion feeding of cells, into which cells can be introduced and extracted after culturing using...... normal pipettes, thus making it readily usable for clinical laboratories. To enhance the functionality of such a chamber we have been investigating the use of active or passive 3D surface modifications. Active modifications involve miniature electrodes able to record electrical or electrochemical signals...... from the cells, while passive modifications involve the presence of a peptide nanotube based scaffold for the cell culturing that mimics the in vivo environment. Two applications involving fluorescent in situ hybridization (FISH) analysis and cancer cell sorting are presented, as examples of further...

  5. Development of cell metabolite analysis on microfluidic platform

    Institute of Scientific and Technical Information of China (English)

    Luyao Lin; Jin-Ming Lin

    2015-01-01

    abstract Cell metabolite analysis is of great interest to analytical chemists and physiologists, with some meta-bolites having been identified as important indicators of major diseases such as cancer. A high-throughput and sensitive method for drug metabolite analysis will largely promote the drug discovery industry. The basic barrier of metabolite analysis comes from the interference of complex components in cell biological system and low abundance of target substances. As a powerful tool in biosample analysis, microfluidic chip enhances the sensitivity and throughput by integrating multiple functional units into one chip. In this review, we discussed three critical steps of establishing functional microfluidic platform for cellular metabolism study. Cell in vitro culture model, on chip sample pretreatment, and microchip combined detectors were described in details and demonstrated by works in five years. And a brief summary was given to discuss the advantages as well as challenges of applying microchip method in cell metabolite and biosample analysis.

  6. SmashCell: A software framework for the analysis of single-cell amplified genome sequences

    DEFF Research Database (Denmark)

    Harrington, Eoghan D; Arumugam, Manimozhiyan; Raes, Jeroen;

    2010-01-01

    SUMMARY: Recent advances in single-cell manipulation technology, whole genome amplification and high-throughput sequencing have now made it possible to sequence the genome of an individual cell. The bioinformatic analysis of these genomes however is far more complicated than the analysis of those...

  7. Gene expression analysis of dendritic/Langerhans cells and Langerhans cell histiocytosis

    NARCIS (Netherlands)

    Rust, Renata; Kluiver, J.; Visser, Lydia; Harms, G.; Blokzijl, T.; Kamps, W.A.; Poppema, Sibrand; van den Berg, Anke

    2006-01-01

    Langerhans cell histiocytosis (LCH) is a neoplastic disorder that results in clonal proliferation of cells with a Langerhans cell (LQ phenotype. The pathogenesis of LCH is still poorly understood. In the present study, serial analysis of gene expression (SAGE) was applied to LCs generated from umbil

  8. Optimization of transparent and reflecting electrodes for amorphous silicon solar cells. Annual technical report, April 1, 1995--March 31, 1996

    Energy Technology Data Exchange (ETDEWEB)

    Gordon, R.G.; Sato, H.; Liang, H.; Liu, X.; Thornton, J. [Harvard Univ., Cambridge, MA (United States)

    1996-08-01

    The general objective is to develop methods to deposit materials which can be used to make more efficient solar cells. The work is organized into three general tasks: Task 1. Develop improved methods for depositing and using transparent conductors of fluorine-doped zinc oxide in amorphous silicon solar cells Task 2. Deposit and evaluate titanium oxide as a reflection-enhancing diffusion barrier between amorphous silicon and an aluminum or silver back-reflector. Task 3. Deposit and evaluate electrically conductive titanium oxide as a transparent conducting layer on which more efficient and more stable superstrate cells can be deposited. About one-third of the current project resources are allocated to each of these three objectives.

  9. Thematic outlook: the technical survey for the fuel cell research network PACO. June 14, 2004 update no. 25; Veille thematique. La veille technique pour le reseau PACO. Actualisation du 14 juin 2004, no. 25

    Energy Technology Data Exchange (ETDEWEB)

    NONE

    2004-07-01

    Summaries of several recent articles are gathered here. They deal with fuel cells, means of transport, hydrogen production and storage. Their different titles are given below: 1)the American plan of de-regulated electric power production with fuel cells 2)application of single wall carbon nano-tubes in fuel cells 3)scenarios of SOFC introduction on the Japanese market 4)advanced SOFC technology and developments at the Siemens Westinghouse firm 5)manufacture and optimal size of a PEMFC cogeneration system for multi-residential application: application to the decomposition strategy 6)analysis of the life cycle of fuel cells using disposal gas 7)technical and economical analysis of a three-generation SOFC system 8)use of APU-FC for an average class tactical lorry, during a use in partial electric power supply or in condition of a silent stand by 9)modelling of the current supply of a lorry at stop: comparison between a idling engine and a APU 10)production of hydrogen and carbon nano-tubes by methane decomposition in a two-stage fluidized bed reactor 11)hydrogen production by auto-thermal reforming of gas-oil 12)simulations of cold starting of a gasoline reformer for mobile applications of fuel cells 13)ethanol production by steam reforming on a Pd/{gamma}Al{sub 2}O{sub 3} trading catalyst 14)control of the CO{sub 2}/(CO+CO{sub 2}) ratio and of the deactivation of the catalyst for the gasoline steam reforming 15)comparison of three integrated processes of partial oxidation producing hydrogen for fuel cells applications 16)search of new methods of the mixture: natural gas/hydrogen production for use in natural gas systems in The Netherlands 17)outline of the energy/hydrogen storage: progress achieved with the different techniques and future prospect of nano-materials 18) hydrogen storage in carbon nano-tubes synthesized by solar way 19)forecast and measurement of the limits of the flammability domain of fuel cells. The references of these articles rae detailed. (O.M.)

  10. Development of molten carbonate fuel cell power plant. Quarterly technical progress report, February 1-April 30, 1980

    Energy Technology Data Exchange (ETDEWEB)

    Peterson, J. R.

    1980-01-01

    Work has been initiated during this first quarter under all four program tasks and by all major participants as described. Task 1.0 activity (establish power plant reference design) concentrated upon definition of user requirements and establishment of power plant subsystem alternatives and characteristics. Task 2.0 work (stack and cell design development and verification) was initiated with a heavy emphasis upon test facilities preparation. A total of 27 laboratory cells were operated during this reporting period and a total of nine cells continued on test at the end of the quarter. Investigation of alternative anode and cathode materials proceeded; a dual-porosity anode was fabricated and tested. Over 10,000 endurance hours on a state-of-the-art cell carried-over from a previous program has been achieved and 1500 hours endurance has been obtained with sheet metal cells. Results presented for electrolyte structure development include comparative data for spray-dried and modified aqueous slurry process powders. Shake-down tests with a rotating disc electrode apparatus for fundamental measurements are described. Concept designs for both prototype and subscale stacks have been identified. Task 3.0 effort (development capability for full-scale stack tests) included preparation of an overall test plan to commercialization for molten carbonate fuel cells and of a functional specification for the tenth-scale stack test facility; drafts of both documents were completed for internal review. Cost-effective manufacturing assessment of available designs and processes was initiated. Task 4.0 work (develop capabilities for operation of stacks on coal-derived gas) included gathering of available contaminants concentration and effects information and preparation of initial projections of contaminant ranges and concentrations. Accomplishments to date and activities planned for the next quarter are described.

  11. Polycrystalline thin film cadmium telluride solar cells fabricated by electrodeposition. Annual technical report, 20 March 1995--19 March 1996

    Energy Technology Data Exchange (ETDEWEB)

    Trefny, J U; Mao, D [Colorado School of Mines, Golden, CO (United States)

    1997-04-01

    The objective of this project is to develop improved processes for fabricating CdTe/CdS polycrystalline thin-film solar cells. Researchers used electrodeposition to form CdTe; electrodeposition is a non-vacuum, low-cost technique that is attractive for economic, large-scale production. During the past year, research and development efforts focused on several steps that are most critical to the fabricating high-efficiency CdTe solar cells. These include the optimization of the CdTe electrodeposition process, the effect of pretreatment of CdS substrates, the post-deposition annealing of CdTe, and back-contact formation using Cu-doped ZnTe. Systematic investigations of these processing steps have led to a better understanding and improved performance of the CdTe-based cells. Researchers studied the structural properties of chemical-bath-deposited CdS thin films and their growth mechanisms by investigating CdS samples prepared at different deposition times; investigated the effect of CdCl{sub 2} treatment of CdS films on the photovoltaic performance of CdTe solar cells; studied Cu-doped ZnTe as a promising material for forming stable, low-resistance contacts to the p-type CdTe; and investigated the effect of CdTe and CdS thickness on the photovoltaic performance of the resulting cells. As a result of their systematic investigation and optimization of the processing conditions, researchers improved the efficiency of CdTe/CdS cells using ZnTe back-contact and electrodeposited CdTe. The best CdTe/CdS cell exhibited a V{sub oc} of 0.778 V, a J{sub sc} of 22.4 mA/cm{sup 2}, a FF of 74%, and an efficiency of 12.9% (verified at NREL). In terms of individual parameters, researchers obtained a V{sub oc} over 0.8 V and a FF of 76% on other cells.

  12. Single Cell Analysis: From Technology to Biology and Medicine.

    Science.gov (United States)

    Pan, Xinghua

    2014-01-01

    Single-cell analysis heralds a new era that allows "omics" analysis, notably genomics, transcriptomics, epigenomics and proteomics at the single-cell level. It enables the identification of the minor subpopulations that may play a critical role in a biological process of a population of cells, which conventionally are regarded as homogeneous. It provides an ultra-sensitive tool to clarify specific molecular mechanisms and pathways and reveal the nature of cell heterogeneity. It also facilitates the clinical investigation of patients when a very low quantity or a single cell is available for analysis, such as noninvasive prenatal diagnosis and cancer screening, and genetic evaluation for in vitro fertilization. Within a few short years, single-cell analysis, especially whole genomic sequencing and transcriptomic sequencing, is becoming robust and broadly accessible, although not yet a routine practice. Here, with single cell RNA-seq emphasized, an overview of the discipline, progresses, and prospects of single-cell analysis and its applications in biology and medicine are given with a series of logic and theoretical considerations.

  13. Technical challenges of sparing infrahyoid swallowing organs at risk in oropharynx squamous cell cancer treated with IMRT

    Energy Technology Data Exchange (ETDEWEB)

    Morley, Lyndon, E-mail: lyndon.morley@rmp.uhn.on.ca [Radiation Medicine Program, Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario (Canada); Tsang, Shirley W.S. [Radiation Medicine Program, Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario (Canada); Breen, Stephen L.; Waldron, John N. [Radiation Medicine Program, Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario (Canada); Department of Radiation Oncology, University of Toronto, Toronto, Ontario (Canada); Maganti, Manjula; Pintilie, Melania [Radiation Medicine Program, Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario (Canada); Dawson, Laura A.; Ringash, Jolie [Radiation Medicine Program, Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario (Canada); Department of Radiation Oncology, University of Toronto, Toronto, Ontario (Canada); Huang, Shao Hui [Radiation Medicine Program, Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario (Canada); Kim, John [Radiation Medicine Program, Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario (Canada); Department of Radiation Oncology, University of Toronto, Toronto, Ontario (Canada)

    2014-07-01

    This study reports clinical performance in the sparing of infrahyoid swallowing organs at risk (SWOARs) in oropharynx cancer intensity-modulated radiation therapy (IMRT) plans. Rates of meeting dose-volume planning goals are reported and compared with geometry-based estimates of what is achievable. This study also develops 3 measures of target-SWOAR geometry and tests their usefulness in providing geometry-based dose-volume planning goals. A total of 50 oropharynx cancer IMRT plans were reviewed. Success rates in meeting institutional dose-volume goals were determined for the glottic larynx (G), postcricoid pharynx (P), and esophagus (E). The following 3 measures of target-SWOAR geometry were investigated as methods of identifying geometry-based planning goals: presence of gross disease in neck levels 3 to 4, target-SWOAR overlap, and a 3-dimensional (3D) measure of target-SWOAR geometry. Locally advanced disease was predominant in this patient population with target volumes overlapping SWOARs in 68% to 98% of cases. Clinical rates of success in meeting dose-volume goals varied by SWOAR (16% to 82%) but compared well with estimated potentially achievable rates in most cases (14% average difference between clinical and potential). Cases grouped by the presence of levels 3 to 4 neck nodes or target-SWOAR overlap did not have significantly different SWOAR doses. Cases grouped using a 3D measure of target-SWOAR geometry differed significantly, providing useful geometry-based planning goals (e.g., mean Glottis dose <45 Gy was achieved 19%, 44%, or 81% of the time in each of 3 groups). This study describes the technical challenge of sparing SWOARs and investigates several potential methods for grouping cases to assist with treatment plan evaluation. Quantifying the 3-D relationship between the targets and SWOARs is a promising way of approaching this complex problem. Data presented in this paper may be useful to evaluate treatment plans using objective geometry

  14. Numerical Simulation and Scaling Analysis of Cell Printing

    Science.gov (United States)

    Qiao, Rui; He, Ping

    2011-11-01

    Cell printing, i.e., printing three dimensional (3D) structures of cells held in a tissue matrix, is gaining significant attention in the biomedical community. The key idea is to use inkjet printer or similar devices to print cells into 3D patterns with a resolution comparable to the size of mammalian cells. Achieving such a resolution in vitro can lead to breakthroughs in areas such as organ transplantation. Although the feasibility of cell printing has been demonstrated recently, the printing resolution and cell viability remain to be improved. Here we investigate a unit operation in cell printing, namely, the impact of a cell-laden droplet into a pool of highly viscous liquids. The droplet and cell dynamics are quantified using both direct numerical simulation and scaling analysis. These studies indicate that although cell experienced significant stress during droplet impact, the duration of such stress is very short, which helps explain why many cells can survive the cell printing process. These studies also revealed that cell membrane can be temporarily ruptured during cell printing, which is supported by indirect experimental evidence.

  15. Single-cell transcriptome analysis of endometrial tissue

    Science.gov (United States)

    Krjutškov, K.; Katayama, S.; Saare, M.; Vera-Rodriguez, M.; Lubenets, D.; Samuel, K.; Laisk-Podar, T.; Teder, H.; Einarsdottir, E.; Salumets, A.; Kere, J.

    2016-01-01

    STUDY QUESTION How can we study the full transcriptome of endometrial stromal and epithelial cells at the single-cell level? SUMMARY ANSWER By compiling and developing novel analytical tools for biopsy, tissue cryopreservation and disaggregation, single-cell sorting, library preparation, RNA sequencing (RNA-seq) and statistical data analysis. WHAT IS KNOWN ALREADY Although single-cell transcriptome analyses from various biopsied tissues have been published recently, corresponding protocols for human endometrium have not been described. STUDY DESIGN, SIZE, DURATION The frozen-thawed endometrial biopsies were fluorescence-activated cell sorted (FACS) to distinguish CD13-positive stromal and CD9-positive epithelial cells and single-cell transcriptome analysis performed from biopsied tissues without culturing the cells. We studied gene transcription, applying a modern and efficient RNA-seq protocol. In parallel, endometrial stromal cells were cultured and global expression profiles were compared with uncultured cells. PARTICIPANTS/MATERIALS, SETTING, METHODS For method validation, we used two endometrial biopsies, one from mid-secretory phase (Day 21, LH+8) and another from late-secretory phase (Day 25). The samples underwent single-cell FACS sorting, single-cell RNA-seq library preparation and Illumina sequencing. MAIN RESULTS AND THE ROLE OF CHANCE Here we present a complete pipeline for single-cell gene-expression studies, from clinical sampling to statistical data analysis. Tissue manipulation, starting from disaggregation and cell-type-specific labelling and ending with single-cell automated sorting, is managed within 90 min at low temperature to minimize changes in the gene expression profile. The single living stromal and epithelial cells were sorted using CD13- and CD9-specific antibodies, respectively. Of the 8622 detected genes, 2661 were more active in cultured stromal cells than in biopsy cells. In the comparison of biopsy versus cultured cells, 5603

  16. Comparative analysis of cell death induction by Taurolidine in different malignant human cancer cell lines

    Directory of Open Access Journals (Sweden)

    Ritter Peter R

    2010-03-01

    Full Text Available Abstract Background Taurolidine (TRD represents an anti-infective substance with anti-neoplastic activity in many malignant cell lines. So far, the knowledge about the cell death inducing mechanisms and pathways activated by TRD is limited. The aim of this study was therefore, to perform a comparative analysis of cell death induction by TRD simultaneously in different malignant cell lines. Materials and methods Five different malignant cell lines (HT29/Colon, Chang Liver/Liver, HT1080/fibrosarcoma, AsPC-1/pancreas and BxPC-3/pancreas were incubated with increasing concentrations of TRD (100 μM, 250 μM and 1000 μM for 6 h and 24 h. Cell viability, apoptosis and necrosis were analyzed by FACS analysis (Propidiumiodide/AnnexinV staining. Additionally, cells were co-incubated with the caspase Inhibitor z-VAD, the radical scavenger N-Acetylcystein (NAC and the Gluthation depleting agent BSO to examine the contribution of caspase activation and reactive oxygen species in TRD induced cell death. Results All cell lines were susceptible to TRD induced cell death without resistance toward this anti-neoplastic agent. However, the dose response effects were varying largely between different cell lines. The effect of NAC and BSO co-treatment were highly different among cell lines - suggesting a cell line specific involvement of ROS in TRD induced cell death. Furthermore, impact of z-VAD mediated inhibition of caspases was differing strongly among the cell lines. Conclusion This is the first study providing a simultaneous evaluation of the anti-neoplastic action of TRD across several malignant cell lines. The involvement of ROS and caspase activation was highly variable among the five cell lines, although all were susceptible to TRD induced cell death. Our results indicate, that TRD is likely to provide multifaceted cell death mechanisms leading to a cell line specific diversity.

  17. Dissection of Cell Division Processes in the One Cell Stage Caenorhabditis elegans Embryo by Mutational Analysis

    OpenAIRE

    Gönczy, Pierre; Schnabel, Heinke; Kaletta, Titus; Amores, Ana Duran; Hyman, Tony; Schnabel, Ralf

    1999-01-01

    To identify novel components required for cell division processes in complex eukaryotes, we have undertaken an extensive mutational analysis in the one cell stage Caenorhabditis elegans embryo. The large size and optical properties of this cell permit observation of cell division processes with great detail in live specimens by simple differential interference contrast (DIC) microscopy. We have screened an extensive collection of maternal-effect embryonic lethal mutations on chromosome III wi...

  18. TECHNICAL REPORT

    Energy Technology Data Exchange (ETDEWEB)

    ADAMS, RICHARD D., PhD.

    2011-04-06

    Cadmium selenide nanoparticles and nanoclusters were prepared and added to polymer solar cells to improve their photon capture ability. These nanoparticles did exhibit some beneficial effects on the photon conversion efficiencies of selected polymer solar cells. Ternary bulk heterojunction systems based on composites of methyl viologen-doped, CdSe nanoparticles blended with poly (3-hexothiopene) (P3HT) and 6, 6-phenyl C{sub 61}-butyric acid methyl ester (PCBM) were also tested. It was found that the devices with methyl viologen-doped CdSe nanoparticles do produce more photocurrent in a region surrounding the absorption peak of the particles (560 to 660nm) when compared to pristine P3HT:PCBM devices. Gold nanorods were also prepared and tested in some solar cells. These nanorods did produce a very small enhancement in photon absorbance, but the observed increase the photon conversion efficiency was not sufficient to make the effort worthwhile. Our goals were (1) to prepare cadmium sulfide and cadmium selenide clusters and nanoparticles to be tested as photon absorbers to enhance the photon conversion efficiency of polymer solar polymer solar cells and (2) to prepare gold and silver nanorods to be added to polymer solar cells to enhance their photon capture capability. The cadmium sulfide and cadmium selenide nanoparticles and some new nanoclusters were prepared. The cadmium selenide nanoparticles were also tested in solar cells and did exhibit some positive effects when they were combined with certain co-absorbing polymers. Due to solubility problems that were not solved in the available time, the new nanoclusters were not tested in solar cells. Ternary bulk heterojunction systems based on composites of methyl viologen doped, CdSe nanoparticles blended with poly (3-hexothiopene) (P3HT) and 6, 6-phenyl C61-butyric acid methyl ester (PCBM) have been examined in detail. The methyl viologen was added to promote charge separation of the initially formed excitons. It was

  19. Final technical report for project titled Quantitative Characterization of Cell Aggregation/Adhesion as Predictor for Distribution and Transport of Microorganisms in Subsurface Environment

    Energy Technology Data Exchange (ETDEWEB)

    Gu, April Z. [Northeastern Univ., Boston, MA (United States); Wan, Kai-tak [Northeastern Univ., Boston, MA (United States)

    2014-09-02

    -surface interactions is essential for the field. To tackle this, we have developed a number of new Bio-nanomechanical techniques, including reflection interference contrast microscopy (RICM) and bio-AFM (Atomic Force Microscopy), for cell adhesion-detachment measurement of the long-range surface interactions, in combination with mathematical modeling, which would allow us to characterize the mechanical behavior from single cell to multi-cell aggregate, critical thresholds for large scale coaggregation and transportation of cells and aggregates in the presence of long range inter-surface forces etc. Although some technical and mathematical challenges remain, the preliminary results promise great breakthrough potential. In this study, we investigated the cellular surface characteristics of representative bio-remediating microorganisms relevant to DOE IFRC (Integrated Field-Scale Subsurface Research Challenges) sites and their transport behaviors in porous media, aiming to draw a groundbreaking correlation between the micro-scale genetic and biological origin-based cell surface properties, the consequent mechanical adhesion and aggregation behaviors, and the macro-scale microbial mobility and retention in porous media, which are unavailable in the literature. The long-term goal is to significantly improve the mechanistic and quantitative understanding of microbial mobility, sorption, and transport within reactive transport models as needed to manipulate subsurface contaminant fate and transport predictions.

  20. TECHNICAL COORDINATION

    CERN Multimedia

    Austin Ball

    2013-01-01

      Since the last report, much visible progress has been made, as the LS1 programme approaches the halfway point. From early October, technical and safety shift-crew have been present around the clock, allowing detectors to stay switched on overnight, ensuring that safety systems are operational and instructions for non-expert shift-crew are clear. LS1 progress Throughout the summer, whilst the solenoid vacuum tank and YB0 surfaces were accessible, an extensive installation programme took place to prepare for Tracker colder operation and the PLT installation, in 2014, the Phase 1 Pixel Tracker installation, in 2016–’17, and the HCAL Phase 1 upgrade completion, ending in LS2. This included pipework for N2 or dry air to flush the Tracker bulkhead region, many sensors to monitor temperature and dew point in the Tracker and its service channels, heating wires outside the Tracker cooling bundles, supports for the new vacuum-jacketed, concentric, CO2 Pixel cooling lines, the PLT cool...

  1. TECHNICAL COORDINATION

    CERN Multimedia

    A. Ball and W. Zeuner

    2012-01-01

      UXC + detectors As explained in detail in the November 2011 bulletin, the bellows unit at −18.5 m from the CMS interaction point was identified as a prime candidate for the regularly occurring pressure spikes which occasionally led to sustained severe background conditions in 2011, affecting dead time and data quality. Similar regions in LHC with vacuum instabilities were observed to be close to bellows, which radiography showed to have distorted RF-fingers — on removal, they proved to have been severely overheated. The plans for the Year-End Technical Stop were adapted to prioritise radiography of the bellows at 16 m to 18 m either end of CMS. Excellent work by the beam pipe, survey and heavy mechanical teams allowed the X-rays to be taken as planned on 20th December, showing that the bellow at −18.5m had an obvious non-conformity. The RF-fingers were found inside the end of the opposing flared pipe instead of outside. In addition, the overlap between fingers and...

  2. Guam Initial Technical Assessment Report

    Energy Technology Data Exchange (ETDEWEB)

    Baring-Gould, I.; Conrad, M.; Haase, S.; Hotchkiss, E.; McNutt, P.

    2011-04-01

    Under an interagency agreement, funded by the Department of Interior's (DOI) Office of Insular Affairs (OIA), the National Renewable Energy Laboratory (NREL) was tasked to deliver technical assistance to the island of Guam by conducting an island initial technical assessment that would lay out energy consumption and production data and establish a baseline. This assessment will be used to conduct future analysis and studies by NREL that will estimate energy efficiency and renewable energy potential for the island of Guam.

  3. Bioinformatics approaches to single-cell analysis in developmental biology.

    Science.gov (United States)

    Yalcin, Dicle; Hakguder, Zeynep M; Otu, Hasan H

    2016-03-01

    Individual cells within the same population show various degrees of heterogeneity, which may be better handled with single-cell analysis to address biological and clinical questions. Single-cell analysis is especially important in developmental biology as subtle spatial and temporal differences in cells have significant associations with cell fate decisions during differentiation and with the description of a particular state of a cell exhibiting an aberrant phenotype. Biotechnological advances, especially in the area of microfluidics, have led to a robust, massively parallel and multi-dimensional capturing, sorting, and lysis of single-cells and amplification of related macromolecules, which have enabled the use of imaging and omics techniques on single cells. There have been improvements in computational single-cell image analysis in developmental biology regarding feature extraction, segmentation, image enhancement and machine learning, handling limitations of optical resolution to gain new perspectives from the raw microscopy images. Omics approaches, such as transcriptomics, genomics and epigenomics, targeting gene and small RNA expression, single nucleotide and structural variations and methylation and histone modifications, rely heavily on high-throughput sequencing technologies. Although there are well-established bioinformatics methods for analysis of sequence data, there are limited bioinformatics approaches which address experimental design, sample size considerations, amplification bias, normalization, differential expression, coverage, clustering and classification issues, specifically applied at the single-cell level. In this review, we summarize biological and technological advancements, discuss challenges faced in the aforementioned data acquisition and analysis issues and present future prospects for application of single-cell analyses to developmental biology.

  4. Studies of Basic Electronic Properties of CdTe-Based Solar Cells and Their Evolution During Processing and Stress; Annual Technical Report, 1 November 2005 - 31 October 2006

    Energy Technology Data Exchange (ETDEWEB)

    Beach, J.; Seymour, F. H.; Kaydanov, V. I.; Ohno, T. R.

    2007-01-01

    We describe the results of our continuing study of deep electronic states controlling open-circuit voltage in CdTe/CdS thin-film solar cells (Task 1). The study includes: (1) analysis of factors affecting trap signatures derived from admittance spectroscopy and capacitance transients measurements, such as activation-energy capture cross-sections and trap-density estimates, and (2) comparative studies of cells received from four different sources and prepared with significant variations in cell structure and processing procedures.

  5. Process Development and Basic Studies of Electrochemically Deposited CdTe-Based Solar Cells; Annual Technical Report, Phase I: May 15, 1998 - May 14, 1999

    Energy Technology Data Exchange (ETDEWEB)

    Kaydanov, V.I.; Ohno, T.R.

    2000-08-25

    The project describes long-term research and development issues related to polycrystalline thin-film solar cells. The general research approach is based on combining activities aimed at improvement of cell performance and stability with activities aimed at increasing fundamental understanding of the properties of materials making up the cells: CdTe, CdS, multi-layer back contact, and transparent conducting oxide (TCO) front contact. The authors emphasize the relation between structural and electronic material properties and various processing procedures, as well as the microscopic mechanisms responsible for the cell performance and its degradation. Major results and conclusions of this project include: (1) Stress tests of the cells under various stress conditions revealed conditions providing the most severe degrading of different cell parameters; (2) Consecutive stress testing under different bias revealed some reversible effects; (3) Preliminary analysis of the data obtained demonstrated a significant role of electromigration of the charged defects/impurities; (4) Some new approaches for the cell characterization and the data analysis were developed and checked experimentally; (5) New stress test experiments were planned for continued studies of degradation mechanisms.

  6. High-efficiency thin-film cadmium telluride photovoltaic cells. Annual technical report, January 20, 1996--January 19, 1997

    Energy Technology Data Exchange (ETDEWEB)

    Compaan, A D; Bohn, R G; Contreras-Puente, G [Univ. of Toledo, OH (United States)

    1997-08-01

    The University of Toledo photovoltaics group has been instrumental in developing rf sputtering for CDs/CdTe thin-film solar cells. During the third phase of the present contract our work focussed on efforts to determine factors which limit the efficiency in our {open_quotes}all-sputtered{close_quotes} thin-film CdTe solar cells on soda-lime glass. We find that our all-sputtered cells, which are deposited at substantially lower temperature than those by sublimation or vapor deposition, require less aggressive CdCl{sub 2} treatments than do other deposition techniques and this is presumably related to CDs/CdTe interdiffusion. The CDs/CdTe interdiffusion process has been studied by several methods, including photoluminescence and capacitance-voltage measurements. Furthermore, we have deposited special thin bilayer films on quartz and borosilicate glass. Interdiffusion in these thin bilayers have been probed by Rutherford backscattering, with collaborators at Case Western Reserve University, and grazing incidence x-ray scattering (GIXS), with collaborators at the University at Buffalo and Brookhaven National Lab. Also, in order better to understand the properties of the ternary alloy material, we used laser physical vapor deposition to prepare a series of CdS{sub x}Te{sub 1-x} films on borosilicate glass. The composition of the alloy films was determined by wavelength dispersive x-ray spectroscopy at NREL. These films are currently being investigated by us and other groups at NREL and IEC.

  7. Analysis of undergraduate cell biology contents in Brazilian public universities.

    Science.gov (United States)

    Mermelstein, Claudia; Costa, Manoel Luis

    2017-04-01

    The enormous amount of information available in cell biology has created a challenge in selecting the core concepts we should be teaching our undergraduates. One way to define a set of essential core ideas in cell biology is to analyze what a specific cell biology community is teaching their students. Our main objective was to analyze the cell biology content currently being taught in Brazilian universities. We collected the syllabi of cell biology courses from public universities in Brazil and analyzed the frequency of cell biology topics in each course. We also compared the Brazilian data with the contents of a major cell biology textbook. Our analysis showed that while some cell biology topics such as plasma membrane and cytoskeleton was present in ∼100% of the Brazilian curricula analyzed others such as cell signaling and cell differentiation were present in only ∼35%. The average cell biology content taught in the Brazilian universities is quite different from what is presented in the textbook. We discuss several possible explanations for these observations. We also suggest a list with essential cell biology topics for any biological or biomedical undergraduate course. The comparative discussion of cell biology topics presented here could be valuable in other educational contexts.

  8. Unraveling cell processes: interference imaging interwoven with data analysis

    DEFF Research Database (Denmark)

    Brazhe, Nadezda; Brazhe, Alexey; Pavlov, A N;

    2006-01-01

    The paper presents results on the application of interference microscopy and wavelet-analysis for cell visualization and studies of cell dynamics. We demonstrate that interference imaging of erythrocytes can reveal reorganization of the cytoskeleton and inhomogenity in the distribution of hemoglo......The paper presents results on the application of interference microscopy and wavelet-analysis for cell visualization and studies of cell dynamics. We demonstrate that interference imaging of erythrocytes can reveal reorganization of the cytoskeleton and inhomogenity in the distribution...... properties differ from cell type to cell type and depend on the cellular compartment. Our results suggest that low frequency variations (0.1-0.6 Hz) result from plasma membrane processes and that higher frequency variations (20-26 Hz) are related to the movement of vesicles. Using double-wavelet analysis, we...... study the modulation of the 1 Hz rhythm in neurons and reveal its changes under depolarization and hyperpolarization of the plasma membrane. We conclude that interference microscopy combined with wavelet analysis is a useful technique for non-invasive cell studies, cell visualization, and investigation...

  9. FINAL/ SCIENTIFIC TECHNICAL REPORT

    Energy Technology Data Exchange (ETDEWEB)

    McDonald, Henry; Singh, Suminderpal

    2006-08-28

    The overall objective of the Chattanooga fuel cell demonstrations project was to develop and demonstrate a prototype 5-kW grid-parallel, solid oxide fuel cell (SOFC) system that co-produces hydrogen, based on Ion America’s technology. The commercial viability of the 5kW SOFC system was tested by transporting, installing and commissioning the SOFC system at the Alternative Energy Laboratory at the University of Tennessee – Chattanooga. The system also demonstrated the efficiency and the reliability of the system running on natural gas. This project successfully contributed to the achievement of DOE technology validation milestones from the Technology Validation section of the Hydrogen, Fuel Cells and Infrastructure Technologies Program Multi-Year Research, Development and Demonstration Plan. Results of the project can be found in the final technical report.

  10. Photovoltage analysis of a heterojunction solar cell

    Institute of Scientific and Technical Information of China (English)

    Xiong Chao; Yao Ruo-He; Geng Kui-Wei

    2011-01-01

    According to the p-n junction model of Shockley, the relationship between the equilibrium carrier concentrations of n-type and p-type semiconductors on the edges of the depletion region of a p-n junction solar cell is analysed. The calculation results show that the photovoltage can exceed the built-in voltage for a special kind of heterojunction solar cell. When the photovoltage exceeds the built-in voltage under illumination, the dark current and the photocurrent are impeded by the peak of voltage barrier at the interface and the expression of the total Ⅰ-Ⅴ characteristic is given.

  11. Potential of BODIPY-cholesterol for analysis of cholesterol transport and diffusion in living cells

    DEFF Research Database (Denmark)

    Wüstner, Daniel; Lund, Frederik Wendelboe; Röhrl, Clemens

    2016-01-01

    Cholesterol is an abundant and important lipid component of cellular membranes. Analysis of cholesterol transport and diffusion in living cells is hampered by the technical challenge of designing suitable cholesterol probes which can be detected for example by optical microscopy. One strategy...... is to use intrinsically fluorescent sterols, as dehydroergosterol (DHE), having minimal chemical alteration compared to cholesterol but giving low fluorescence signals in the UV region of the spectrum. Alternatively, one can use dye-tagged cholesterol analogs and in particular BODIPY-cholesterol (BChol......), whose synthesis and initial characterization was pioneered by Robert Bittman. Here, we give a general overview of the properties and applications but also limitations of BODIPY-tagged cholesterol probes for analyzing intracellular cholesterol trafficking. We describe our own experiences...

  12. Micromagnetic Cancer Cell Immobilization and Release for Real-Time Single Cell Analysis

    Science.gov (United States)

    Jaiswal, Devina; Rad, Armin Tahmasbi; Nieh, Mu-Ping; Claffey, Kevin P.; Hoshino, Kazunori

    2017-04-01

    Understanding the interaction of live cells with macromolecules is crucial for designing efficient therapies. Considering the functional heterogeneity found in cancer cells, real-time single cell analysis is necessary to characterize responses. In this study, we have designed and fabricated a microfluidic channel with patterned micromagnets which can temporarily immobilize the cells during analysis and release them after measurements. The microchannel is composed of plain coverslip top and bottom panels to facilitate easy microscopic observation and undisturbed application of analytes to the cells. Cells labeled with functionalized magnetic beads were immobilized in the device with an efficiency of 90.8±3.6%. Since the micromagnets are made of soft magnetic material (Ni), they released cells when external magnetic field was turned off from the channel. This allows the reuse of the channel for a new sample. As a model drug analysis, the immobilized breast cancer cells (MCF7) were exposed to fluorescent lipid nanoparticles and association and dissociation were measured through fluorescence analysis. Two concentrations of nanoparticles, 0.06 μg/ml and 0.08 μg/ml were tested and time lapse images were recorded and analyzed. The microfluidic device was able to provide a microenvironment for sample analysis, making it an efficient platform for real-time analysis.

  13. Single-cell transcriptome analysis of fish immune cells provides insight into the evolution of vertebrate immune cell types

    Science.gov (United States)

    Ferreira, Lauren; Macaulay, Iain C.; Stubbington, Michael J.T.

    2017-01-01

    The immune system of vertebrate species consists of many different cell types that have distinct functional roles and are subject to different evolutionary pressures. Here, we first analyzed conservation of genes specific for all major immune cell types in human and mouse. Our results revealed higher gene turnover and faster evolution of trans-membrane proteins in NK cells compared with other immune cell types, and especially T cells, but similar conservation of nuclear and cytoplasmic protein coding genes. To validate these findings in a distant vertebrate species, we used single-cell RNA sequencing of lck:GFP cells in zebrafish and obtained the first transcriptome of specific immune cell types in a nonmammalian species. Unsupervised clustering and single-cell TCR locus reconstruction identified three cell populations, T cells, a novel type of NK-like cells, and a smaller population of myeloid-like cells. Differential expression analysis uncovered new immune-cell–specific genes, including novel immunoglobulin-like receptors, and neofunctionalization of recently duplicated paralogs. Evolutionary analyses confirmed the higher gene turnover of trans-membrane proteins in NK cells compared with T cells in fish species, suggesting that this is a general property of immune cell types across all vertebrates. PMID:28087841

  14. Metabolism and cell shape in cancer: a fractal analysis.

    Science.gov (United States)

    D'Anselmi, Fabrizio; Valerio, Mariacristina; Cucina, Alessandra; Galli, Luca; Proietti, Sara; Dinicola, Simona; Pasqualato, Alessia; Manetti, Cesare; Ricci, Giulia; Giuliani, Alessandro; Bizzarri, Mariano

    2011-07-01

    Fractal analysis in cancer cell investigation provided meaningful insights into the relationship between morphology and phenotype. Some reports demonstrated that changes in cell shape precede and trigger dramatic modifications in both gene expression and enzymatic function. Nonetheless, metabolomic pattern in cells undergoing shape changes have been not still reported. Our study was aimed to investigate if modifications in cancer cell morphology are associated to relevant transition in tumour metabolome, analyzed by nuclear magnetic resonance spectroscopy and principal component analysis. MCF-7 and MDA-MB-231 breast cancer cells, exposed to an experimental morphogenetic field, undergo a dramatic change in their membrane profiles. Both cell lines recover a more rounded shape, loosing spindle and invasive protrusions, acquiring a quite "normal" morphology. This result, quantified by fractal analysis, shows that normalized bending energy (a global shape characterization expressing the amount of energy needed to transform a specific shape into its lowest energy state) decreases after 48 h. Later on, a significant shift from a high to a low glycolytic phenotype was observed on both cell lines: glucose flux begins to drop off at 48 h, leading to reduced lactate accumulation, and fatty acids and citrate synthesis slow-down after 72 h. Moreover, de novo lipidogenesis is inhibited and nucleotide synthesis is reduced, as indicated by the positive correlation between glucose and formate. In conclusion, these data indicate that the reorganization of cell membrane architecture, induced by environmental cues, is followed by a relevant transition of the tumour metabolome, suggesting cells undergo a dramatic phenotypic reversion.

  15. KIT mutation analysis in mast cell neoplasms

    DEFF Research Database (Denmark)

    Arock, M; Sotlar, K; Akin, C;

    2015-01-01

    Although acquired mutations in KIT are commonly detected in various categories of mastocytosis, the methodologies applied to detect and quantify the mutant type and allele burden in various cells and tissues are poorly defined. We here propose a consensus on methodologies used to detect KIT...

  16. Hyperstructures, genome analysis and I-cells

    DEFF Research Database (Denmark)

    Amar, P.; Ballet, P.; Barlovatz-Meimon, G.;

    2002-01-01

    New concepts may prove necessary to profit from the avalanche of sequence data on the genome, transcriptome, proteome and interactome and to relate this information to cell physiology. Here, we focus on the concept of large activity-based structures, or hyperstructures, in which a variety of type...

  17. Segregation analysis of urothelial cell carcinoma.

    NARCIS (Netherlands)

    Aben, K.K.H.; Baglietto, L.; Baffoe-Bonnie, A.B.; Coebergh, J.W.W.; Bailey-Wilson, J.E.; Trink, B.; Verbeek, A.L.M.; Schoenberg, M.P.; Witjes, J.A.; Kiemeney, L.A.L.M.

    2006-01-01

    A family history of urothelial cell carcinoma (UCC) confers an almost two-fold increased risk of developing UCC. It is unknown whether (part of) this aggregation of UCC has a Mendelian background. We performed complex segregation analyses on 1193 families ascertained through a proband with UCC of th

  18. Final technical report for Phenomic Analysis of Natural and Induced Variation in Brachypodium Distachyon DE-SC0001526

    Energy Technology Data Exchange (ETDEWEB)

    Vogel, John P. [USDA ARS Western Regional Research Center, Albany, NY (United States)

    2014-12-17

    The goal of this project was to apply high-throughput, non-destructive phenotyping (phenomics) to collections of natural variants and induced mutants of the model grass Brachypodium distachyon and characterize a small subset of that material in detail. B. distachyon is well suited to this phenomic approach because its small size and rapid generation time allow researchers to grow many plants under carefully controlled conditions. In addition, the simple diploid genetics, high quality genome sequence and existence of numerous experimental tools available for B. distachyon allow us to rapidly identify genes affecting specific phenotypes. Our phenomic analysis revealed great diversity in biofuel-relevant traits like growth rate, biomass and photosynthetic rate. This clearly demonstrated the feasibility of applying a phenomic approach to the model grass B. distachyon. We also demonstrated the utility of B. distachyon for studying mature root system, something that is virtually impossible to do with biomass crops. We showed tremendous natural variation in root architecture that can potentially be used to design crops with superior nutrient and water harvesting capability. Finally, we demonstrated the speed with which we can link specific genes to specific phenotypes by studying two mutants in detail. Importantly, in both cases, the specific biological lessons learned were grass-specific and could not have been learned from a dicot model system. Furthermore, one of the genes affects cell wall integrity and thus may be a useful target in the context of biomass crop improvement. Ultimately, all this information can be used to accelerate the creation of improved biomass crops.

  19. Electron-Ion Dynamics with Time-Dependent Density Functional Theory: Towards Predictive Solar Cell Modeling: Final Technical Report

    Energy Technology Data Exchange (ETDEWEB)

    Maitra, Neepa [Hunter College City University of New York, New York, NY (United States)

    2016-07-14

    This project investigates the accuracy of currently-used functionals in time-dependent density functional theory, which is today routinely used to predict and design materials and computationally model processes in solar energy conversion. The rigorously-based electron-ion dynamics method developed here sheds light on traditional methods and overcomes challenges those methods have. The fundamental research undertaken here is important for building reliable and practical methods for materials discovery. The ultimate goal is to use these tools for the computational design of new materials for solar cell devices of high efficiency.

  20. Determination of optimum electrolyte composition for molten carbonate fuel cells. Quarterly technical progress report, October--December 1987

    Energy Technology Data Exchange (ETDEWEB)

    Yuh, C.Y.; Pigeaud, A.

    1987-12-31

    The goal of this program is the development of a molten carbonate fuel cell (MCFC) anode which is more tolerant of sulfur contaminants in the fuel than the current state-of-the-art nickel-based anode structures. This program addresses two different but related aspects of the sulfur contamination problem. The primary aspect is concerned with the development of a sulfur tolerant electrocatalyst for the fuel oxidation reaction. A secondary issue is the development of a sulfur tolerant water-gas-shift reaction catalyst and an investigation of potential steam reforming catalysts which also have some sulfur tolerant capabilities. These two aspects are being addressed as two separate tasks.

  1. Single-cell analysis of growth and cell division of the anaerobe Desulfovibrio vulgaris Hildenborough

    Directory of Open Access Journals (Sweden)

    Anouchka eFievet

    2015-12-01

    Full Text Available Recent years have seen significant progress in understanding basic bacterial cell cycle properties such as cell growth and cell division. While characterization and regulation of bacterial cell cycle is quite well documented in the case of fast growing aerobic model organisms, no data has been so far reported for anaerobic bacteria. This lack of information in anaerobic microorganisms can mainly be explained by the absence of molecular and cellular tools such as single cell microscopy and fluorescent probes usable for anaerobes and essential to study cellular events and/or subcellular localization of the actors involved in cell cycle.In this study, single-cell microscopy has been adapted to study for the first time, in real time, the cell cycle of a bacterial anaerobe, Desulfovibrio vulgaris Hildenborough (DvH. This single-cell analysis provides mechanistic insights into the cell division cycle of DvH, which seems to be governed by the recently discussed so-called incremental model that generates remarkably homogeneous cell sizes. Furthermore, cell division was reversibly blocked during oxygen exposure. This may constitute a strategy for anaerobic cells to cope with transient exposure to oxygen that they may encounter in their natural environment, thereby contributing to their aerotolerance. This study lays the foundation for the first molecular, single-cell assay that will address factors that cannot otherwise be resolved in bulk assays and that will allow visualization of a wide range of molecular mechanisms within living anaerobic cells.

  2. Technical approach document

    Energy Technology Data Exchange (ETDEWEB)

    1989-12-01

    The Uranium Mill Tailings Radiation Control Act (UMTRCA) of 1978, Public Law 95-604 (PL95-604), grants the Secretary of Energy the authority and responsibility to perform such actions as are necessary to minimize radiation health hazards and other environmental hazards caused by inactive uranium mill sites. This Technical Approach Document (TAD) describes the general technical approaches and design criteria adopted by the US Department of Energy (DOE) in order to implement remedial action plans (RAPS) and final designs that comply with EPA standards. It does not address the technical approaches necessary for aquifer restoration at processing sites; a guidance document, currently in preparation, will describe aquifer restoration concerns and technical protocols. This document is a second revision to the original document issued in May 1986; the revision has been made in response to changes to the groundwater standards of 40 CFR 192, Subparts A--C, proposed by EPA as draft standards. New sections were added to define the design approaches and designs necessary to comply with the groundwater standards. These new sections are in addition to changes made throughout the document to reflect current procedures, especially in cover design, water resources protection, and alternate site selection; only minor revisions were made to some of the sections. Sections 3.0 is a new section defining the approach taken in the design of disposal cells; Section 4.0 has been revised to include design of vegetated covers; Section 8.0 discusses design approaches necessary for compliance with the groundwater standards; and Section 9.0 is a new section dealing with nonradiological hazardous constituents. 203 refs., 18 figs., 26 tabs.

  3. 激光标刻系统技术参数分析%Analysis Technical Parameters of The Laser Marking System

    Institute of Scientific and Technical Information of China (English)

    肖彬; 黄焰

    2016-01-01

    介绍了激光标刻的应用领域和加工原理,并详细说明目前三种常见激光标刻方式的特点。以振镜扫描式激光标刻为例,分析激光标刻系统技术参数对加工效果的影响,以及分析影响标刻质量的主要参数。%The application field and the processing principle of laser marking system is introduced in this paper.And the characteristics of three common laser marking methods at present are detailed.Taking the galvanometer scanning of laser marking as an example,analysis technical parameters of the laser marking system about the influences of processing effect.And analysis the main parameters of marking quality.

  4. Energy conservation analysis using Fuzzy logic considering technical and environmental aspects; Analise da conservacao de energia utilizando logica fuzzy considerando aspectos tecnicos e o meio ambiente

    Energy Technology Data Exchange (ETDEWEB)

    Haddad, Jamil; Yamachita, Roberto Akira; Guardia, Eduardo Crestana [Universidade Federal de Itajuba (UNIFEI), MG (Brazil). Centro de Excelencia em Eficiencia Energetica; Reis, Alexandre de Souza Rodrigues dos; Pinto, Luiz Fernando do Monte [Furnas Centrais Eletricas, Rio de Janeiro, RJ (Brazil)

    2008-07-01

    This article presents the results obtained in the Project of Research and Development of Furnas Centrais Eletricas, which was accomplished with the partnership of the Fundacao de Pesquisa e Assessoramento a Industria - FUPAI. This project analyzed technically and economically the pollution caused in the electric net by the use, in growing scale, of nonlinear loads, such as: compact fluorescent lamps, microwave ovens, VHS systems, DVD, sound systems, etc. It was also analyzed the environmental impact caused by these lamps due to the presence of the mercury; and as complement to the traditional theory of economical analysis, a tool of economical analysis was developed using the theory of fuzzy sets for the uncertainty variables or of difficult quantification. (author)

  5. Dielectrophoretic capture and genetic analysis of single neuroblastoma tumor cells

    Directory of Open Access Journals (Sweden)

    Erica L Carpenter

    2014-07-01

    Full Text Available Our understanding of the diversity of cells that escape the primary tumor and seed micrometastases remains rudimentary, and approaches for studying circulating and disseminated tumor cells have been limited by low throughput and sensitivity, reliance on single parameter sorting, and a focus on enumeration rather than phenotypic and genetic characterization. Here we utilize a highly sensitive microfluidic and dielectrophoretic approach for the isolation and genetic analysis of individual tumor cells. We employed fluorescence labeling to isolate 208 single cells from spiking experiments conducted with 11 cell lines, including 8 neuroblastoma cell lines, and achieved a capture sensitivity of 1 tumor cell per 106 white blood cells. Sample fixation or freezing had no detectable effect on cell capture. Point mutations were accurately detected in the whole genome amplification product of captured single tumor cells but not in negative control white blood cells. We applied this approach to capture 144 single tumor cells from 10 bone marrow samples from patients suffering from neuroblastoma. In this pediatric malignancy, high-risk patients often exhibit wide-spread hematogenous metastasis, but access to primary tumor can be difficult or impossible. Here we used flow-based sorting to pre-enrich samples with tumor involvement below 0.02%. For all patients for whom a mutation in the Anaplastic Lymphoma Kinase gene had already been detected in their primary tumor, the same mutation was detected in single cells from their marrow. These findings demonstrate a novel, non-invasive, and adaptable method for the capture and genetic analysis of single tumor cells from cancer patients.

  6. Photovoltaic mechanisms in polycrystalline thin film silicon solar cells. Quarterly technical progress report No. 1, July 30-October 31, 1980

    Energy Technology Data Exchange (ETDEWEB)

    Sopori, B.

    1980-07-30

    Major accomplishments during the first quarter of the contract period are reported. Small area diode fabrication and analysis has been continued. This technique has further been applied to many RTR ribbons. An optical technique for determination of crystallite orientations has been placed in operation. This technique has many distinct advantages. These are: (1) rapid; (2) can be set-up very inexpensively; (3) well suited for polycrystalline substrates of small grain size; and (4) can easily characterize twins. Accuracies obtained with this technique are about the same as that of the Laue technique. A technique to qualitatively evaluate grain boundary activity in unprocessed substrates has been used and valuable results obtained. Further analysis is being done to use this technique for quantitative evaluation. A major study of G.B. orientation effects is underway. Initial results on RTR ribbons have shown a good correlation of G.B. barrier height with misorientation (tilt boundaries).

  7. Acid test of joint technical and biological measures in slope stabilisation - Impact analysis of the heavy rainstorm event in August 2005

    Science.gov (United States)

    Graf, F.; Böll, A.

    2009-04-01

    The persisting and heavy rainstorms from 20th to 22nd August in 2005 resulted in loss of human lives and tremendous damage on infrastructure all over Switzerland. Many of the measures taken hitherto to protect against such natural hazards were stressed to their limits or even beyond due to water saturation of the soils and extreme discharges of the torrents. This particular configuration offered the possibility to investigate the reliability of technical and biological measures taken within the scope of slope stabilisation, torrent and gully control. In the context of a joint project the ancient sliding area "Schwandrübi" in Dallenwil (Switzerland) providing joint technical and biological measures was chosen to address aspects concerning the reliability of technical supporting structures, the development of biological measures in the course of time and their performance under the extreme impact as well as the effects of biological measures on the stability of slopes. During 1981 and 1982 joint technical and biological measures had been taken on a large scale with minor follow-ups shortly after to stabilise the "Schwandrübi". The underlying strategy was based on several pilot surveys as thorough soil analysis, e.g. grain size distribution and determination of the angle of internal friction (Φ') related to the porosity (n) and the dry unit weight (γ), respectively. Basically, the spatial arrangement of the gabions was in accordance with the theoretical guidelines. However, based on the angle of internal friction (Φ') determined on the loose moraine soil material, it was not possible to meet the soil mechanical criterion of inclination between the constructions in all cases. Regardless of the extreme impact during the rainstorm (~100-year event), no serious damage occurred neither on the roughly 25-year old gabions nor on the torrent control structures. The recalculated peak discharge in the outlet channel was ~60 m3s-1 superimposed by high bed load

  8. Advanced energy analysis of high temperature fuel cell systems

    NARCIS (Netherlands)

    De Groot, A.

    2004-01-01

    In this thesis the performance of high temperature fuel cell systems is studied using a new method of exergy analysis. The thesis consists of three parts: ⢠In the first part a new analysis method is developed, which not only considers the total exergy losses in a unit operation, but which distingu

  9. Design, Implementation and Analysis of Learner-Centered Guided In-Service Programme for Technical Teacher Education

    Directory of Open Access Journals (Sweden)

    Tiia Rüütmann

    2014-03-01

    Full Text Available The design of learner-centered guided in-service program for technical teacher education has been launched at Estonian Centre for Engineering Pedagogy at Tallinn University of Technology. The aim of the program has been facilitating the initial in-service teaching experience, involving mentoring, peer support and cognitive apprenticeship. The emphasis has been on teaching for knowledge application, including the choice of content and processes, motivation, reflection, problem-solving, choice of educational technology, effective teaching methods, teaching models and strategies ཿ concepts, tools and procedures of the field of engineering, organized in ways enabling teachers to formulate real world problems, apply and solve them. The program has been implemented since 2012 and covers IGIP curriculum requirements. In the present article the designed program is described and analyzed.

  10. Analysis of concentrating PV-T systems for the commercial/industrial sector. Volume III. Technical issues and design guidance

    Energy Technology Data Exchange (ETDEWEB)

    Schwinkendorf, W.E.

    1984-09-01

    This report provide appropriate guidance for addressing the major technical issues associated with the design and installation of a photovoltaic-thermal (PV-T) system. Nomographs are presented for developing preliminary sizing and costing, and issues associated with specific components and the overall design of the electrical and mechanical system are discussed. SAND82-7157/2 presents a review of current PV-T technology and operating systems and a study of potential PV-T applications. Detailed PV-T system designs for three selected applications and the results of a trade-off study for these applications are presented in SAND82-7157/4. A summary of the major results of this entire study and conclusions concerning PV-T systems and applications is presented in SAND82-7157/1.

  11. Analysis and Methane Gas Separations Studies for City of Marsing, Idaho An Idaho National Laboratory Technical Assistance Program Study

    Energy Technology Data Exchange (ETDEWEB)

    Christopher Orme

    2012-08-01

    Introduction and Background Large amounts of methane in well water is a wide spread problem in North America. Methane gas from decaying biomass and oil and gas deposits escape into water wells typically through cracks or faults in otherwise non-porous rock strata producing saturated water systems. This methane saturated water can pose several problems in the delivery of drinking water. The problems range from pumps vapor locking (cavitating), to pump houses exploding. The City of Marsing requested Idaho National Laboratory (INL) to assist with some water analyses as well as to provide some engineering approaches to methane capture through the INL Technical Assistance Program (TAP). There are several engineering approaches to the removal of methane and natural gas from water sources that include gas stripping followed by compression and/or dehydration; membrane gas separators coupled with dehydration processes, membrane water contactors with dehydration processes.

  12. Transcriptome analysis of mouse stem cells and early embryos.

    Directory of Open Access Journals (Sweden)

    Alexei A Sharov

    2003-12-01

    Full Text Available Understanding and harnessing cellular potency are fundamental in biology and are also critical to the future therapeutic use of stem cells. Transcriptome analysis of these pluripotent cells is a first step towards such goals. Starting with sources that include oocytes, blastocysts, and embryonic and adult stem cells, we obtained 249,200 high-quality EST sequences and clustered them with public sequences to produce an index of approximately 30,000 total mouse genes that includes 977 previously unidentified genes. Analysis of gene expression levels by EST frequency identifies genes that characterize preimplantation embryos, embryonic stem cells, and adult stem cells, thus providing potential markers as well as clues to the functional features of these cells. Principal component analysis identified a set of 88 genes whose average expression levels decrease from oocytes to blastocysts, stem cells, postimplantation embryos, and finally to newborn tissues. This can be a first step towards a possible definition of a molecular scale of cellular potency. The sequences and cDNA clones recovered in this work provide a comprehensive resource for genes functioning in early mouse embryos and stem cells. The nonrestricted community access to the resource can accelerate a wide range of research, particularly in reproductive and regenerative medicine.

  13. Transcriptome Analysis of Mouse Stem Cells and Early Embryos

    Science.gov (United States)

    Sharov, Alexei A; Piao, Yulan; Matoba, Ryo; Dudekula, Dawood B; Qian, Yong; VanBuren, Vincent; Falco, Geppino; Martin, Patrick R; Stagg, Carole A; Bassey, Uwem C; Wang, Yuxia; Carter, Mark G; Hamatani, Toshio; Aiba, Kazuhiro; Akutsu, Hidenori; Sharova, Lioudmila; Tanaka, Tetsuya S; Kimber, Wendy L; Yoshikawa, Toshiyuki; Jaradat, Saied A; Pantano, Serafino; Nagaraja, Ramaiah; Boheler, Kenneth R; Taub, Dennis; Hodes, Richard J; Longo, Dan L; Schlessinger, David; Keller, Jonathan; Klotz, Emily; Kelsoe, Garnett; Umezawa, Akihiro; Vescovi, Angelo L; Rossant, Janet; Kunath, Tilo; Hogan, Brigid L. M; Curci, Anna; D'Urso, Michele; Kelso, Janet; Hide, Winston

    2003-01-01

    Understanding and harnessing cellular potency are fundamental in biology and are also critical to the future therapeutic use of stem cells. Transcriptome analysis of these pluripotent cells is a first step towards such goals. Starting with sources that include oocytes, blastocysts, and embryonic and adult stem cells, we obtained 249,200 high-quality EST sequences and clustered them with public sequences to produce an index of approximately 30,000 total mouse genes that includes 977 previously unidentified genes. Analysis of gene expression levels by EST frequency identifies genes that characterize preimplantation embryos, embryonic stem cells, and adult stem cells, thus providing potential markers as well as clues to the functional features of these cells. Principal component analysis identified a set of 88 genes whose average expression levels decrease from oocytes to blastocysts, stem cells, postimplantation embryos, and finally to newborn tissues. This can be a first step towards a possible definition of a molecular scale of cellular potency. The sequences and cDNA clones recovered in this work provide a comprehensive resource for genes functioning in early mouse embryos and stem cells. The nonrestricted community access to the resource can accelerate a wide range of research, particularly in reproductive and regenerative medicine. PMID:14691545

  14. Analysis of Intracellular Calcium Signaling in Human Embryonic Stem Cells.

    Science.gov (United States)

    Péntek, Adrienn; Pászty, Katalin; Apáti, Ágota

    2016-01-01

    Measurement of changes in intracellular calcium concentration is one of the most common and useful tools for studying signal transduction pathways or cellular responses in basic research and drug screening purposes as well. Increasing number of such applications using human pluripotent stem cells and their derivatives requires development of calcium signal measurements for this special cell type. Here we describe a modified protocol for analysis of calcium signaling events in human embryonic stem cells, which can be used for other pluripotent cell types (such as iPSC) or their differentiated offspring as well.

  15. Technical Readiness and Gaps Analysis of Commercial Optical Materials and Measurement Systems for Advanced Small Modular Reactors

    Energy Technology Data Exchange (ETDEWEB)

    Anheier, Norman C.; Suter, Jonathan D.; Qiao, Hong (Amy); Andersen, Eric S.; Berglin, Eric J.; Bliss, Mary; Cannon, Bret D.; Devanathan, Ramaswami; Mendoza, Albert; Sheen, David M.

    2013-08-06

    This report intends to support Department of Energy’s Office of Nuclear Energy (DOE-NE) Nuclear Energy Research and Development Roadmap and industry stakeholders by evaluating optical-based instrumentation and control (I&C) concepts for advanced small modular reactor (AdvSMR) applications. These advanced designs will require innovative thinking in terms of engineering approaches, materials integration, and I&C concepts to realize their eventual viability and deployability. The primary goals of this report include: 1. Establish preliminary I&C needs, performance requirements, and possible gaps for AdvSMR designs based on best available published design data. 2. Document commercial off-the-shelf (COTS) optical sensors, components, and materials in terms of their technical readiness to support essential AdvSMR in-vessel I&C systems. 3. Identify technology gaps by comparing the in-vessel monitoring requirements and environmental constraints to COTS optical sensor and materials performance specifications. 4. Outline a future research, development, and demonstration (RD&D) program plan that addresses these gaps and develops optical-based I&C systems that enhance the viability of future AdvSMR designs. The development of clean, affordable, safe, and proliferation-resistant nuclear power is a key goal that is documented in the Nuclear Energy Research and Development Roadmap. This roadmap outlines RD&D activities intended to overcome technical, economic, and other barriers, which currently limit advances in nuclear energy. These activities will ensure that nuclear energy remains a viable component to this nation’s energy security.

  16. Development of a Wide Bandgap Cell for Thin Film Tandem Solar Cells: Final Technical Report, 6 November 2003 - 5 January 2007

    Energy Technology Data Exchange (ETDEWEB)

    Shafarman, W.; McCandless, B.

    2008-08-01

    The objective of this research program was to develop approaches for a transparent wide-bandgap cell to be used in a thin-film tandem polycrystalline solar cell that can ultimately attain 25% efficiency. Specific goals included the research and development of Cu(InGa)(SeS)2 and Cd1-xZnxTe alloys with a bandgap from 1.5 to 1.8 eV, demonstrating the potential of a 15% cell efficiency with a transparent contact, and supporting the High Performance PV Program. This Final Report presents results that emphasize the 3rd phase of the program.

  17. High resolution, low cost solar cell contact development. Quarterly technical progress and schedule report, September 28, 1980

    Energy Technology Data Exchange (ETDEWEB)

    Mardesich, N.

    1980-01-01

    The scope of the contract covers the development and evaluation of forming solar cell collector grid contacts by the MIDFILM process. This is a proprietary process developed by the Ferro Corporation which is a subcontractor for the program. The MIDFILM process attains line resolution characteristics of photoresist methods with processing related to screen printing. The surface to be processed is first coated with a thin layer of photoresist material. Upon exposure to ultraviolet light through a suitable mask, the resist in the non-pattern area cross-links and becomes hard. The unexposed pattern areas remain tacky. The conductor material is applied in the form of a dry mixture of metal and frit particles which adhere to the tacky pattern area. The assemblage is then fired to ash the photopolymer and sinter the fritted conductor powder. Effort was concentrated during this period on the establishment, optimization and identification of problem areas of the MIDFILM process. Progress is reported. (WHK)

  18. Cell Stability Analysis of Conventional 6T Dynamic 8T SRAM Cell in 45NM Technology

    Directory of Open Access Journals (Sweden)

    K. Dhanumjaya

    2012-04-01

    Full Text Available A SRAM cell must meet requirements for operation in submicron/nano ranges. The scaling of CMOS technology has significant impact on SRAM cell -- random fluctuation of electrical characteristics and substantial leakage current. In this paper we present dynamic column based power supply 8 T SRAM cell and comparing the proposed SRAM cell with respect to conventional SRAM 6T in various aspects. To verify read stability and write ability analysis we use N-curve metric. Simulation results affirmed that proposed 8T SRAM cell achieved improved read stability, read current, and leakage current in 45nm Technology comparing with conventional 6T SRAM using cadence virtuoso tool.

  19. Cell Stability Analysis of Conventional 6T Dynamic 8T SRAM Cell in 45NM Technology

    Directory of Open Access Journals (Sweden)

    K.Padmaraju

    2012-05-01

    Full Text Available A SRAM cell must meet requirements for operation in submicron/nano ranges. The scaling of CMOS technology has significant impact on SRAM cell -- random fluctuation of electrical characteristics andsubstantial leakage current. In this paper we present dynamic column based power supply 8T SRAM cell and comparing the proposed SRAM cell with respect to conventional SRAM 6T in various aspects. To verify read stability and write ability analysis we use N-curve metric. Simulation results affirmed that proposed 8T SRAM cell achieved improved read stability, read current, and leakage current in 45nm Technology comparing with conventional 6T SRAM using cadence virtuoso tool.

  20. Gene expression analysis of in vivo fluorescent cells.

    Directory of Open Access Journals (Sweden)

    Konstantin Khodosevich

    Full Text Available BACKGROUND: The analysis of gene expression for tissue homogenates is of limited value because of the considerable cell heterogeneity in tissues. However, several methods are available to isolate a cell type of interest from a complex tissue, the most reliable one being Laser Microdissection (LMD. Cells may be distinguished by their morphology or by specific antigens, but the obligatory staining often results in RNA degradation. Alternatively, particular cell types can be detected in vivo by expression of fluorescent proteins from cell type-specific promoters. METHODOLOGY/PRINCIPAL FINDINGS: We developed a technique for fixing in vivo fluorescence in brain cells and isolating them by LMD followed by an optimized RNA isolation procedure. RNA isolated from these cells was of equal quality as from unfixed frozen tissue, with clear 28S and 18S rRNA bands of a mass ratio of approximately 2ratio1. We confirmed the specificity of the amplified RNA from the microdissected fluorescent cells as well as its usefulness and reproducibility for microarray hybridization and quantitative real-time PCR (qRT-PCR. CONCLUSIONS/SIGNIFICANCE: Our technique guarantees the isolation of sufficient high quality RNA obtained from specific cell populations of the brain expressing soluble fluorescent marker, which is a critical prerequisite for subsequent gene expression studies by microarray analysis or qRT-PCR.

  1. Dynamic metabolic flux analysis of plant cell wall synthesis.

    Science.gov (United States)

    Chen, Xuewen; Alonso, Ana P; Shachar-Hill, Yair

    2013-07-01

    The regulation of plant cell wall synthesis pathways remains poorly understood. This has become a bottleneck in designing bioenergy crops. The goal of this study was to analyze the regulation of plant cell wall precursor metabolism using metabolic flux analysis based on dynamic labeling experiments. Arabidopsis T87 cells were cultured heterotrophically with (13)C labeled sucrose. The time course of ¹³C labeling patterns in cell wall precursors and related sugar phosphates was monitored using liquid chromatography tandem mass spectrometry until steady state labeling was reached. A kinetic model based on mass action reaction mechanisms was developed to simulate the carbon flow in the cell wall synthesis network. The kinetic parameters of the model were determined by fitting the model to the labeling time course data, cell wall composition, and synthesis rates. A metabolic control analysis was performed to predict metabolic regulations that may improve plant biomass composition for biofuel production. Our results describe the routes and rates of carbon flow from sucrose to cell wall precursors. We found that sucrose invertase is responsible for the entry of sucrose into metabolism and UDP-glucose-4-epimerase plays a dominant role in UDP-Gal synthesis in heterotrophic Aradidopsis cells under aerobic conditions. We also predicted reactions that exert strong regulatory influence over carbon flow to cell wall synthesis and its composition.

  2. Technical infrastructure monitoring at CERN

    CERN Document Server

    Stowisek, Jan; Suwalska, Anna

    2006-01-01

    The Technical Infrastructure Monitoring system (TIM) is used to monitor and control CERN's technical services from the CERN Control Centre (CCC). The system's primary function is to provide CCC operators with reliable real-time information about the state of the laboratory's extensive and widely distributed technical infrastructure. TIM is also used to monitor all general services required for the operation of CERN's accelerator complex and the experiments. A flexible data acquisition mechanism allows TIM to interface with a wide range of technically diverse installations, using industry standard protocols wherever possible and custom designed solutions where needed. The complexity of the data processing logic, including persistence, logging, alarm handling, command execution and the evaluation of datadriven business rules is encapsulated in the system's business layer. Users benefit from a suite of advanced graphical applications adapted to operations (synoptic views, alarm consoles, data analysis tools etc....

  3. Dynamic transcriptional signature and cell fate analysis reveals plasticity of individual neural plate border cells.

    Science.gov (United States)

    Roellig, Daniela; Tan-Cabugao, Johanna; Esaian, Sevan; Bronner, Marianne E

    2017-03-29

    The 'neural plate border' of vertebrate embryos contains precursors of neural crest and placode cells, both defining vertebrate characteristics. How these lineages segregate from neural and epidermal fates has been a matter of debate. We address this by performing a fine-scale quantitative temporal analysis of transcription factor expression in the neural plate border of chick embryos. The results reveal significant overlap of transcription factors characteristic of multiple lineages in individual border cells from gastrula through neurula stages. Cell fate analysis using a Sox2 (neural) enhancer reveals that cells that are initially Sox2+ cells can contribute not only to neural tube but also to neural crest and epidermis. Moreover, modulating levels of Sox2 or Pax7 alters the apportionment of neural tube versus neural crest fates. Our results resolve a long-standing question and suggest that many individual border cells maintain ability to contribute to multiple ectodermal lineages until or beyond neural tube closure.

  4. Photovoltaic mechanisms in polycrystalline thin film solar cells. Quarterly technical progress report No. 3, April 1-June 30, 1979

    Energy Technology Data Exchange (ETDEWEB)

    Storti, G.; Johnson, S.; Lin, H.C.; Armstrong, R.W.

    1979-01-01

    The vertical junction effect in polysilicon solar cells (due to dopant penetration along grain boundaries) is investigated using a cylindrical grain model. The model shows that for small grain radii the short circuit current can increase above the single crystal value if the grain boundaries collect carriers and that th short-circuit current approaches the single crystal value as the grain size increases. The spatial dependence of the dark I-V characteristic is investigated by fabricating small diodes on a polysilicon wafer. Dark I-V characteristics are measured and illuminated characteristics are projected for each of the diodes. The diode characteristics are given together with SEM micrographs of the diodes and the effect of areal inhomogemety is investigated. The relative orientations of adjacent crystal grains and the nature of the boundaries between grains are studied by x-ray diffraction, optical microscopy and SEM methods. Orientation data is presented along with electron channelling patterns obtained from individual grains. A conclusion is drawn on the origin of sinuous grain boundaries.

  5. Controlled cadmium telluride thin films for solar-cell applications. Final technical report, June 1, 1980-May 31, 1981

    Energy Technology Data Exchange (ETDEWEB)

    Das, M.B.; Krishnaswamy, S.V.

    1981-06-01

    The objectives of this contract were to carry out a systematic study on the preparation and characterization of rf-sputtered CdTe thin films in order to establish reproducibility of the films with good electrical characteristics and to demonstrate the feasibility of fabricating various types of junctions and ohmic contacts with reproducible characteristics and finally to optimize the most promising solar cell structure in order to achieve an efficiency of 6% or higher. Efforts have been directed to the control of various sputtering parameters in order to obtain good quality films. The structure, crystallographic, compositional and electrical properties of cadmium telluride films sputtered over a wide range of conditions have been evaluated. A series of doping experiments have been carried out using primarily Cd, Te, In, as the n-type dopants and Cu as the p-type dopant. Of these dopants, indium doping provided films with which S.B. junctions can be obtained for further electrical characterization. Use of cadmium overpressure during CdTe:In sputtering has improved the film characteristics. Ion Beam Sputtering was attempted as an alternative technique for film preparation. For lack of time and due to a number of mechanical failures, no significant results could be obtained.

  6. Technical Efficiency Analysis of Wheat Farmers in Henan Province%河南省农户小麦生产技术效率分析

    Institute of Scientific and Technical Information of China (English)

    王时雨

    2014-01-01

    Using the farmer’s research data of Henan Hebi,Xuchang,Zhumadian City,using data envelopment analysis (DEA) analyzed the technical efficiency of wheat farmers to produce these three cities,the results showed that the highest technical efficiency of wheat farmers in Hebi City,Xuchang times the Zhumadian lowest,and most farmers three cities have the potential to expand the scale of planting. On this basis,raised funds to increase investment financial support for agriculture,the transformation of a large low-yielding fields,force farmland water conservancy facilities construction.%采用河南省鹤壁、许昌、驻马店三市农户的调研数据,运用数据包络分析法(DEA)分析这三个市的农户小麦生产技术效率,结果表明鹤壁市农户小麦生产技术效率最高,许昌次之,驻马店最低,而且三个市的大部分农户都有扩大种植规模的潜力。并在此基础生提出加大财政支农资金的投入力度,改造中低产田大,力进行农田水利设施建设的对策和建议。

  7. Mutational Analysis of Merkel Cell Carcinoma

    Energy Technology Data Exchange (ETDEWEB)

    Erstad, Derek J. [Department of Surgery, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114 (United States); Cusack, James C. Jr., E-mail: jcusack@mgh.harvard.edu [Division of Surgical Oncology, Harvard Medical School, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114 (United States)

    2014-10-17

    Merkel cell carcinoma (MCC) is an aggressive cutaneous neuroendocrine malignancy that is associated with a poor prognosis. The pathogenesis of MCC is not well understood, and despite a recent plethora of mutational analyses, we have yet to find a set of signature mutations implicated in the majority of cases. Mutations, including TP53, Retinoblastoma and PIK3CA, have been documented in subsets of patients. Other mechanisms are also likely at play, including infection with the Merkel cell polyomavirus in a subset of patients, dysregulated immune surveillance, epigenetic alterations, aberrant protein expression, posttranslational modifications and microRNAs. In this review, we summarize what is known about MCC genetic mutations and chromosomal abnormalities, and their clinical significance. We also examine aberrant protein function and microRNA expression, and discuss the therapeutic and prognostic implications of these findings. Multiple clinical trials designed to selectively target overexpressed oncogenes in MCC are currently underway, though most are still in early phases. As we accumulate more molecular data on MCC, we will be better able to understand its pathogenic mechanisms, develop libraries of targeted therapies, and define molecular prognostic signatures to enhance our clinicopathologic knowledge.

  8. Proteome of human colon cancer stem cells: A comparative analysis

    Institute of Scientific and Technical Information of China (English)

    Jian Zou; Xiao-Feng Yu; Zhi-Jun Bao; Jie Dong

    2011-01-01

    AIM: To isolate and identify the biological characteristics of human colon cancer stem cells (SW1116 cells) and further study their proteome. METHODS: SW1116 cells were isolated and cultured with a serum-free medium (SFM). Sphere formation was assayed to observe the formation of colon cancer stem cell spheres. SW1116 cells were inoculated into a serum-containing medium for observing their differentiation characteristics. Proliferation curve and cross-resistance of SW1116 cells to different drugs were detected by MTT. Percentage of SP cells in SW1116 cells was detected with Hoechst33342 staining. Telomerase activity in SW1116cells was checked by polymerase chain reaction (PCR)-enzyme linked immunosorbent assay. Expressions of stem cell relevant genes and proteins were detected by reverse transcription-PCR and Western blot, respectively. Total protein was isolated from SW1116 cells by two-dimensional gel electrophoresis (2-DE) and differentially expressed proteins were identified by tandem mass spectrometry (MALDI-TOF/TOF). RESULTS: The isolated SW1116 cells presented as spheroid and suspension growths in SFM with a strong self-renewal, proliferation, differentiation and drug-resistance ability. The percentage of SP cells in SW1116 cells was 38.9%. The SW1116 cells co-expressed the CD133 and CD29 proteins. The telomerase activity in SW1116 cells was increased. The expressions of different stem cell relevant genes and proteins were detected. The proteomic analysis showed that the 26 protein spots were differently expressed in SW1116 cells and 10 protein spots were identified as ubiquitin fusiondegradation 1-like protein, nuclear chloride channel protein, tubulin b, Raichu404X, stratifin, F-actin capping protein a-1 subunit, eukaryotic translation elongation factor 1 delta isoform 2, hypothetical protein, glyceraldehyde-3-phosphate dehydrogenase and guanine nucleotide binding protein b polypeptide 2-like 1, respectively. CONCLUSION: SW1116 cells are biologically

  9. Cell Wall Microstructure Analysis Implicates Hemicellulose Polysaccharides in Cell Adhesion in Tomato Fruit Pericarp Parenchyma

    Institute of Scientific and Technical Information of China (English)

    Jose J. Ordaz-Ortiz; Susan E. Marcus; J. Paul Knox

    2009-01-01

    Methods developed to isolate intact cells from both unripe and ripe tomato fruit pericarp parenchyma have allowed the cell biological analysis of polysaccharide epitopes at the surface of separated cells. The LM7 pectic homoga-lacturonan epitope is a marker of the junctions of adhesion planes and intercellular spaces in parenchyma systems. The LM7 epitope persistently marked the former edge of adhesion planes at the surface of cells separated from unripe and ripened tomato fruit and also from fruits with the Cnr mutation. The LM 11 xylan epitope was associated, in sections, with cell walls lining intercellular space but the epitope was not detected at the surface of isolated cells, being lost during cell isolation. The LM15 xyloglucan epitope was present at the surface of cells isolated from unripe fruit in a pattern reflecting the former edge of cell adhesion planes/intercellular space but with gaps and apparent breaks, An equivalent pattern ofLM15 epitope occurrence was revealed at the surface of cells isolated by pectate lyase action but was not present in cells isolated from ripe fruit or from Cnr fruit. In contrast to wild-type cells, the LM5 galactan and LM21 mannan epitopes oc-curred predominantly in positions reflecting intercellular space in Cnr, suggesting a concerted alteration in cell wall mi-crostructure in response to this mutation. Galactanase and mannanase, along with pectic homogalacturonan-degrading enzymes, were capable of releasing cells from unripe fruit parenchyma. These observations indicate that hemicellulose polymers are present in architectural contexts reflecting cell adhesion and that several cell wall polysaccharide classes are likely to contribute to cell adhesion/cell separation in tomato fruit pericarp parenchyma.

  10. Extracellular matrix stiffness modulates VEGF calcium signaling in endothelial cells: individual cell and population analysis.

    Science.gov (United States)

    Derricks, Kelsey E; Trinkaus-Randall, Vickery; Nugent, Matthew A

    2015-09-01

    Vascular disease and its associated complications are the number one cause of death in the Western world. Both extracellular matrix stiffening and dysfunctional endothelial cells contribute to vascular disease. We examined endothelial cell calcium signaling in response to VEGF as a function of extracellular matrix stiffness. We developed a new analytical tool to analyze both population based and individual cell responses. Endothelial cells on soft substrates, 4 kPa, were the most responsive to VEGF, whereas cells on the 125 kPa substrates exhibited an attenuated response. Magnitude of activation, not the quantity of cells responding or the number of local maximums each cell experienced distinguished the responses. Individual cell analysis, across all treatments, identified two unique cell clusters. One cluster, containing most of the cells, exhibited minimal or slow calcium release. The remaining cell cluster had a rapid, high magnitude VEGF activation that ultimately defined the population based average calcium response. Interestingly, at low doses of VEGF, the high responding cell cluster contained smaller cells on average, suggesting that cell shape and size may be indicative of VEGF-sensitive endothelial cells. This study provides a new analytical tool to quantitatively analyze individual cell signaling response kinetics, that we have used to help uncover outcomes that are hidden within the average. The ability to selectively identify highly VEGF responsive cells within a population may lead to a better understanding of the specific phenotypic characteristics that define cell responsiveness, which could provide new insight for the development of targeted anti- and pro-angiogenic therapies.

  11. Computerized image analysis of cell-cell interactions in human renal tissue by using multi-channel immunoflourescent confocal microscopy

    Science.gov (United States)

    Peng, Yahui; Jiang, Yulei; Liarski, Vladimir M.; Kaverina, Natalya; Clark, Marcus R.; Giger, Maryellen L.

    2012-03-01

    Analysis of interactions between B and T cells in tubulointerstitial inflammation is important for understanding human lupus nephritis. We developed a computer technique to perform this analysis, and compared it with manual analysis. Multi-channel immunoflourescent-microscopy images were acquired from 207 regions of interest in 40 renal tissue sections of 19 patients diagnosed with lupus nephritis. Fresh-frozen renal tissue sections were stained with combinations of immunoflourescent antibodies to membrane proteins and counter-stained with a cell nuclear marker. Manual delineation of the antibodies was considered as the reference standard. We first segmented cell nuclei and cell membrane markers, and then determined corresponding cell types based on the distances between cell nuclei and specific cell-membrane marker combinations. Subsequently, the distribution of the shortest distance from T cell nuclei to B cell nuclei was obtained and used as a surrogate indicator of cell-cell interactions. The computer and manual analyses results were concordant. The average absolute difference was 1.1+/-1.2% between the computer and manual analysis results in the number of cell-cell distances of 3 μm or less as a percentage of the total number of cell-cell distances. Our computerized analysis of cell-cell distances could be used as a surrogate for quantifying cell-cell interactions as either an automated and quantitative analysis or for independent confirmation of manual analysis.

  12. Mutational Analysis of Cell Types in TSC

    Science.gov (United States)

    2008-01-01

    associated with epilepsy and autism . The generation of two new model systems permits more in-depth analysis of the developmental pathogenesis of TSC and...disability, and autism . TSC1/TSC2 gene mutations lead to developmental alterations in brain structure known as tubers in over 80% of TSC patients. Loss of...that is associated with epilepsy, cognitive disability, and autism . TSC1/TSC2 gene mutations lead to developmental alterations in brain structure

  13. Microfluidic single-cell analysis for systems immunology.

    Science.gov (United States)

    Junkin, Michael; Tay, Savaş

    2014-04-07

    The immune system constantly battles infection and tissue damage, but exaggerated immune responses lead to allergies, autoimmunity and cancer. Discrimination of self from foreign and the fine-tuning of immunity are achieved by information processing pathways, whose regulatory mechanisms are little understood. Cell-to-cell variability and stochastic molecular interactions result in diverse cellular responses to identical signaling inputs, casting doubt on the reliability of traditional population-averaged analyses. Furthermore, dynamic molecular and cellular interactions create emergent properties that change over multiple time scales. Understanding immunity in the face of complexity and noisy dynamics requires time-dependent analysis of single-cells in a proper context. Microfluidic systems create precisely defined microenvironments by controlling fluidic and surface chemistries, feature sizes, geometries and signal input timing, and thus enable quantitative multi-parameter analysis of single cells. Such qualities allow observable dynamic environments approaching in vivo levels of biological complexity. Seamless parallelization of functional units in microfluidic devices allows high-throughput measurements, an essential feature for statistically meaningful analysis of naturally variable biological systems. These abilities recapitulate diverse scenarios such as cell-cell signaling, migration, differentiation, antibody and cytokine production, clonal selection, and cell lysis, thereby enabling accurate and meaningful study of immune behaviors in vitro.

  14. (18)F-FDG PET/CT quantification in head and neck squamous cell cancer: principles, technical issues and clinical applications.

    Science.gov (United States)

    Manca, Gianpiero; Vanzi, Eleonora; Rubello, Domenico; Giammarile, Francesco; Grassetto, Gaia; Wong, Ka Kit; Perkins, Alan C; Colletti, Patrick M; Volterrani, Duccio

    2016-07-01

    (18)F-FDG PET/CT plays a crucial role in the diagnosis and management of patients with head and neck squamous cell cancer (HNSCC). The major clinical applications of this method include diagnosing an unknown primary tumour, identifying regional lymph node involvement and distant metastases, and providing prognostic information. (18)F-FDG PET/CT is also used for precise delineation of the tumour volume for radiation therapy planning and dose painting, and for treatment response monitoring, by detecting residual or recurrent disease. Most of these applications would benefit from a quantitative approach to the disease, but the quantitative capability of (18)F-FDG PET/CT is still underused in HNSCC. Innovations in PET/CT technology promise to overcome the issues that until now have hindered the employment of dynamic procedures in clinical practice and have limited "quantification" to the evaluation of standardized uptake values (SUV), de facto a semiquantitative parameter, the limits of which are well known to the nuclear medicine community. In this paper the principles of quantitative imaging and the related technical issues are reviewed so that professionals involved in HNSCC management can reflect on the advantages of "true" quantification. A discussion is then presented on how semiquantitative information is currently used in clinical (18)F-FDG PET/CT applications in HNSCC, by discussing the improvements that could be obtained with more advanced and "personalized" quantification techniques.

  15. Hydrogen Delivery Technical Team Roadmap

    Energy Technology Data Exchange (ETDEWEB)

    None

    2013-06-01

    The mission of the Hydrogen Delivery Technical Team (HDTT) is to enable the development of hydrogen delivery technologies, which will allow for fuel cell competitiveness with gasoline and hybrid technologies by achieving an as-produced, delivered, and dispensed hydrogen cost of $2-$4 per gallon of gasoline equivalent of hydrogen.

  16. Technical Development of Gamma Scanning for Irradiated Fuel Rod after Upgrade of System in Hot-cell

    Energy Technology Data Exchange (ETDEWEB)

    Ryu, Woo Seog; Kim, Hee Moon; Baik, Seung Je; Yoo, Byung Ok; Choo, Yong Sun

    2007-06-15

    Non-destructive test system was installed at hot-cell(M1) in IMEF(Irradiated Materials Examination Facility) more than 10 years ago for the diametric measurement and gamma scanning of fuel rod. But this system must be needed to be remodeled for the effective operations. In 2006, the system was upgraded for 3 months. The collimator bench can be movable with horizontal direction(x-direction) by motorized system for sectional gamma scanning and 3-dimensional tomography of fuel rod. So, gamma scanning for fuel rod can be detectable by x, y and rotation directions. It may be possible to obtain the radioactivities with radial and axial directions of pellet. This system is good for the series experiments with several positions. Operation of fuel bench and gamma detection program were linked each other by new program tools. It can control detection and bench moving automatically when gamma inspection of fuel rod is carried out with axial or radial positions. Some of electronic parts were added in PLC panel, and operating panel was re-designed for the remote control. To operate the fuel bench by computer, AD converter and some I/O cards were installed in computer. All of software were developed in Windows-XP system instead of DOS system. Control programs were made by visual-C language. After upgrade of system, DUPIC fuel which was irradiated in HANARO research reactor was detected by gamma scanning. The results were good and operation of gamma scanning showed reduced inspection time and easy control of data on series of detection with axial positions. With consideration of ECT(Eddy Current Test) installation, the computer program and hardware were set up as well. But ECT is not installed yet, so we have to check abnormal situation of program and hardware system. It is planned to install ECT in 2007.

  17. Quantitative and integrative analysis of paracrine hepatocyte activation by nonparenchymal cells upon lipopolysaccharide induction.

    Science.gov (United States)

    Beuke, Katharina; Schildberg, Frank A; Pinna, Federico; Albrecht, Ute; Liebe, Roman; Bissinger, Michaela; Schirmacher, Peter; Dooley, Steven; Bode, Johannes G; Knolle, Percy A; Kummer, Ursula; Breuhahn, Kai; Sahle, Sven

    2017-03-01

    Gut-derived bacterial lipopolysaccharides (LPS) stimulate the secretion of tumour necrosis factor (TNF) from liver macrophages (MCs), liver sinusoidal endothelial cells (LSECs) and hepatic stellate cells (HSCs), which control the acute phase response in hepatocytes through activation of the NF-κB pathway. The individual and cooperative impact of nonparenchymal cells on this clinically relevant response has not been analysed in detail due to technical limitations. To gain an integrative view on this complex inter- and intracellular communication, we combined a multiscale mathematical model with quantitative, time-resolved experimental data of different primary murine liver cell types. We established a computational model for TNF-induced NF-κB signalling in hepatocytes, accurately describing dose-responsiveness for physiologically relevant cytokine concentrations. TNF secretion profiles were quantitatively measured for all nonparenchymal cell types upon LPS stimulation. This novel approach allowed the analysis of individual and collective paracrine TNF-mediated NF-κB induction in hepatocytes, revealing strongest effects of MCs and LSECs on hepatocellular NF-κB signalling. Simulations suggest that both cell types act together to maximize the NF-κB pathway response induced by low LPS concentrations (0.1 and 1 ng/mL). Higher LPS concentrations (≥ 5 ng/mL) induced sufficient TNF levels from MCs or LSECs to induce a strong and nonadjustable pathway response. Importantly, these simulations also revealed that the initial cytokine secretion (1-2 h after stimulation) rather than final TNF level (10 h after stimulation) defines the hepatocellular NF-κB response. This raises the question whether the current experimental standard of single high-dose cytokine administration is suitable to mimic in vivo cytokine exposure.

  18. Monolithic solid oxide fuel cell technology advancement for coal-based power generation. Quarterly technical status report, January--March 1992

    Energy Technology Data Exchange (ETDEWEB)

    1992-04-14

    The program is conducted by a team consisting of AiResearch Los Angeles Division of Allied-Signal Aerospace Company and Argonne National Laboratory (ANL). The objective of the program is to advance materials and fabrication methodologies to develop a monolithic solid oxide fuel cell (MSOFC) system capable of meeting performance, life, and cost goals for coal-based power generation. The program focuses on materials research and development, fabrication process development, cell/stack performance testing and characterization, cost and system analysis, and quality development.

  19. Critical analysis of pyrolysis process with cellulosic based municipal waste as renewable source in energy and technical perspective.

    Science.gov (United States)

    Agarwal, Manu; Tardio, James; Venkata Mohan, S

    2013-11-01

    To understand the potential of cellulosic based municipal waste as a renewable feed-stock, application of pyrolysis by biorefinery approach was comprehensively studied for its practicable application towards technical and environmental viability in Indian context. In India, where the energy requirements are high, the pyrolysis of the cellulosic waste shows numerous advantages for its applicability as a potential waste-to-energy technology. The multiple energy outputs of the process viz., bio-gas, bio-oil and bio-char can serve the two major energy sectors, viz., electricity and transportation. The process suits best for high bio-gas and electrical energy production when energy input is satisfied from bio-char in form of steam (scheme-1). The bio-gas generated through the process shows its direct utility as a transportation fuel while the bio-oil produced can serve as fuel or raw material to chemical synthesis. On a commercial scale the process is a potent technology towards sustainable development. The process is self-sustained when operated on a continuous mode.

  20. Copy Number Variation Analysis by Array Analysis of Single Cells Following Whole Genome Amplification.

    Science.gov (United States)

    Dimitriadou, Eftychia; Zamani Esteki, Masoud; Vermeesch, Joris Robert

    2015-01-01

    Whole genome amplification is required to ensure the availability of sufficient material for copy number variation analysis of a genome deriving from an individual cell. Here, we describe the protocols we use for copy number variation analysis of non-fixed single cells by array-based approaches following single-cell isolation and whole genome amplification. We are focusing on two alternative protocols, an isothermal and a PCR-based whole genome amplification method, followed by either comparative genome hybridization (aCGH) or SNP array analysis, respectively.

  1. Single-cell analysis defines the divergence between the innate lymphoid cell lineage and lymphoid tissue-inducer cell lineage.

    Science.gov (United States)

    Ishizuka, Isabel E; Chea, Sylvestre; Gudjonson, Herman; Constantinides, Michael G; Dinner, Aaron R; Bendelac, Albert; Golub, Rachel

    2016-03-01

    The precise lineage relationship between innate lymphoid cells (ILCs) and lymphoid tissue-inducer (LTi) cells is poorly understood. Using single-cell multiplex transcriptional analysis of 100 lymphoid genes and single-cell cultures of fetal liver precursor cells, we identified the common proximal precursor to these lineages and found that its bifurcation was marked by differential induction of the transcription factors PLZF and TCF1. Acquisition of individual effector programs specific to the ILC subsets ILC1, ILC2 and ILC3 was initiated later, at the common ILC precursor stage, by transient expression of mixed ILC1, ILC2 and ILC3 transcriptional patterns, whereas, in contrast, the development of LTi cells did not go through multilineage priming. Our findings provide insight into the divergent mechanisms of the differentiation of the ILC lineage and LTi cell lineage and establish a high-resolution 'blueprint' of their development.

  2. Analysis of lead toxicity in human cells

    Directory of Open Access Journals (Sweden)

    Gillis Bruce S

    2012-07-01

    Full Text Available Abstract Background Lead is a metal with many recognized adverse health side effects, and yet the molecular processes underlying lead toxicity are still poorly understood. Quantifying the injurious effects of lead is also difficult because of the diagnostic limitations that exist when analyzing human blood and urine specimens for lead toxicity. Results We analyzed the deleterious impact of lead on human cells by measuring its effects on cytokine production and gene expression in peripheral blood mononuclear cells. Lead activates the secretion of the chemokine IL-8 and impacts mitogen-dependent activation by increasing the secretion of the proinflammatory cytokines IL-6 and TNF-α and of the chemokines IL-8 and MIP1-α in the presence of phytohemagglutinin. The recorded changes in gene expression affected major cellular functions, including metallothionein expression, and the expression of cellular metabolic enzymes and protein kinase activity. The expression of 31 genes remained elevated after the removal of lead from the testing medium thereby allowing for the measurement of adverse health effects of lead poisoning. These included thirteen metallothionein transcripts, three endothelial receptor B transcripts and a number of transcripts which encode cellular metabolic enzymes. Cellular responses to lead correlated with blood lead levels and were significantly altered in individuals with higher lead content resultantly affecting the nervous system, the negative regulation of transcription and the induction of apoptosis. In addition, we identified changes in gene expression in individuals with elevated zinc protoporphyrin blood levels and found that genes regulating the transmission of nerve impulses were affected in these individuals. The affected pathways were G-protein mediated signaling, gap junction signaling, synaptic long-term potentiation, neuropathic pain signaling as well as CREB signaling in neurons. Cellular responses to lead were

  3. From pre-registration to publication: a non-technical primer for conducting a meta-analysis to synthesize correlational data.

    Science.gov (United States)

    Quintana, Daniel S

    2015-01-01

    Meta-analysis synthesizes a body of research investigating a common research question. Outcomes from meta-analyses provide a more objective and transparent summary of a research area than traditional narrative reviews. Moreover, they are often used to support research grant applications, guide clinical practice, and direct health policy. The aim of this article is to provide a practical and non-technical guide for psychological scientists that outlines the steps involved in planning and performing a meta-analysis of correlational datasets. I provide a supplementary R script to demonstrate each analytical step described in the paper, which is readily adaptable for researchers to use for their analyses. While the worked example is the analysis of a correlational dataset, the general meta-analytic process described in this paper is applicable for all types of effect sizes. I also emphasize the importance of meta-analysis protocols and pre-registration to improve transparency and help avoid unintended duplication. An improved understanding this tool will not only help scientists to conduct their own meta-analyses but also improve their evaluation of published meta-analyses.

  4. TECHNICAL ANALYSIS BY THE JAPANESE CANDLES METHOD ON THE EXAMPLE OF AGRICULTURAL MARKETS ANALIZA TECHNICZNA METODA SWIEC JAPONSKICH NA PRZYKLADZIE RYNK ROLNYCH

    Directory of Open Access Journals (Sweden)

    ANNA JAKUBCZAK

    2013-03-01

    Full Text Available The key goal of work was to study of basic information possibilities of the Japanese Candles method and to present its usefulness to make decisions on agricultural markets. The method is a result of the evolution of methods of technical analysis, which lasted for centuries. The usefulness of this method to make decisions is high, but significantly affect have the ability to interpret the observed signals and taking into account the specificities of agricultural markets.Celem opracowania bylo zbadanie podstawowych mozliwosci informacyjnych metody swiec japonskich oraz przedstawienie jej przydatnosci do podejmowania decyzji na rynkach rolnych. Zaprezentowano zwiezla ewolucje metod analizy technicznej oraz scharakteryzowano podstawowe narzedzia metody swiec japonskich, takie jak objecie bessy i hossy, pojedyncze swiece i ich formacje dajace sygnal do wycofania z rynku, doji , luki oraz formacje kontynuacji trendu.

  5. Oil field decision analysis based on technical-economic indicators; Analise de decisao baseada em indicadores tecnico-economicos para um campo petrolifero

    Energy Technology Data Exchange (ETDEWEB)

    Ravagnani, Ana Teresa Gaspar; Munoz Mazo, Eduin; Schiozer, Denis [Universidade Estadual de Campinas (UNICAMP), SP (Brazil). Dept. de Engenharia de Petroleo. Lab. de Simulacao de Fluxo em Meios Porosos (UNISIM)

    2008-07-01

    This work presents a case study consisting of a synthetic offshore field in deep water and 28 deg API oil representing a gas solution model with water injection without gas cap. Several alternatives of strategies are proposed, regarding different configurations and number of wells, besides different limits of injection and production rates, as well as, completion layers. The objective is to show that different strategies can be obtained according to the indicator chosen by the decision maker. Even when the Net Present Value (NPV) is a very used indicator in the investment analysis, with the utilization of other technical-economical indicators, other investment alternatives different from those proposed when just VPL is utilized, can become feasible. Additionally, other aspects are analyzed, such as the possibility of changes in the production capacity with other oil prices levels. It is also proposed the production strategy optimization in this work, changing the time of production and the injection/production rate limits. (author)

  6. NASA/DOD Aerospace Knowledge Diffusion Research Project. Paper 42: An analysis of the transfer of Scientific and Technical Information (STI) in the US aerospace industry

    Science.gov (United States)

    Kennedy, John M.; Pinelli, Thomas E.; Hecht, Laura F.; Barclay, Rebecca O.

    1994-01-01

    The U.S. aerospace industry has a long history of federal support for research related to its needs. Since the establishment of the National Advisory Committee for Aeronautics (NACA) in 1915, the federal government has provided continuous research support related to flight and aircraft design. This research has contributed to the international preeminence of the U.S. aerospace industry. In this paper, we present a sociological analysis of aerospace engineers and scientists and how their attitudes and behaviors impact the flow of scientific and technical information (STI). We use a constructivist framework to explain the spotty dissemination of federally funded aerospace research. Our research is aimed towards providing federal policymakers with a clearer understanding of how and when federally funded aerospace research is used. This understanding will help policymakers design improved information transfer systems that will aid the competitiveness of the U.S. aerospace industry.

  7. Analysis methodology for economic technical feasibility studies in offshore electrical generation systems; Metodologia de analisis para estudios de factibilidad tecnica economica en sistemas de generacion electrica costa fuera

    Energy Technology Data Exchange (ETDEWEB)

    Fiscal Escalante, Raul [Instituto de Investigaciones Electricas, Cuernavaca, Morelos (Mexico)

    2007-07-01

    An analysis methodology followed in the development of technique-economic feasibility studies of systems of electrical generation in offshore electrical installations is presented, including the obtaining of the curves of the turbine and generator performance, the technical considerations for the formulation of the operation scenes and the calculations of the economic evaluation of a real scenario. [Spanish] Se muestra una metodologia de analisis seguida en el desarrollo de estudios de factibilidad tecnica-economica de sistemas de generacion electrica en instalaciones electricas costa fuera, incluyendo la obtencion de las curvas de comportamiento de la turbina y el generador, las consideraciones tecnicas para la formulacion de los escenarios de operacion y los calculos de la evaluacion economica de un escenario real.

  8. 甲醇制芳烃技术及经济性浅析%Technical and Economic Analysis of Methanol Aromatization Process

    Institute of Scientific and Technical Information of China (English)

    王程俊

    2014-01-01

    甲醇制芳烃(MTA)是以煤炭为起始原料合成优质芳烃的重要途径,对保障国家能源安全、实现石油替代具有战略意义。综述了MTA生产技术的研究进展和产业化进程,对经济性进行了简要分析,对安徽发展MTA提出建议。%Methanol to aromatics process(MTA) is an important way of producing high quality aromatics from coal as the starting material and has strategic importance in national energy security and oil alternative. This paper summaries the research of methanol aromatization technical and industrialization process, makes a brief economic analysis about MTA process. Some conclusion and advice are given in the end.

  9. 地基基础设计方案及技术、经济分析%Foundation Design Program and Its Technical and Economic Analysis

    Institute of Scientific and Technical Information of China (English)

    梅长骞; 张慧琦

    2015-01-01

    Based on a specific project in Shenyang, the foundation design program and its technical and economic analysis are analyzed, and through the comparison of the foundation programs, the prestressed pipe foundation program is considered as the best one, because its cost is the lowest, duration is the shortest, and it is the most safe and reliable.%本文根据沈阳市某具体工程,对该工程地基基础设计方案及技术经济进行了分析,通过基础方案设计比选认为预应力管柱基础方案费用高最低,工期最短,安全可靠。

  10. Clinical utility of chromosomal microarray analysis of DNA from buccal cells: detection of mosaicism in three patients.

    Science.gov (United States)

    Sdano, Mallory R; Vanzo, Rena J; Martin, Megan M; Baldwin, Erin E; South, Sarah T; Rope, Alan F; Allen, William P; Kearney, Hutton

    2014-12-01

    Mosaic chromosomal abnormalities are relatively common. However, mosaicism may be missed due to multiple factors including failure to recognize clinical indications and order appropriate testing, technical limitations of diagnostic assays, or sampling tissue (s) in which mosaicism is either not present, or present at very low levels. Blood leukocytes have long been the "gold standard" sample for cytogenetic analysis; however, the culturing process for routine chromosome analysis can complicate detection of mosaicism since the normal cell line may have a growth advantage in culture, or may not be present in the cells that produce metaphases (the lymphocytes). Buccal cells are becoming increasingly utilized for clinical analyses and are proving to have many advantages. Buccal swabs allow for simple and noninvasive DNA collection. When coupled with a chromosomal microarray that contains single nucleotide polymorphic probes, analysis of buccal cells can maximize a clinician's opportunity to detect cytogenetic mosaicism. We present three cases of improved diagnosis of mosaic aberrations using buccal specimens for chromosomal microarray analysis. In each case, the aberration was either undetectable in blood or present at such a low level it likely could have gone undetected. These cases highlight the limitations of certain laboratory methodologies for identifying mosaicism. We also present practice implications for genetic counselors, including clinic workflow changes and counseling approaches based on increasing use of buccal samples.

  11. Molten carbonate fuel cells. Modeling, analysis, simulation, and control

    Energy Technology Data Exchange (ETDEWEB)

    Sundmacher, K.; Kienle, A. [Max-Planck-Institut fuer Dynamik Komplexer Technischer Systeme, Magdeburg (Germany); Pesch, H.J. [Bayreuth Univ. (Germany). Lehrstuhl fuer Ingenieurmathematik; Berndt, J.F. [IPF Beteiligungsgesellschaft Berndt KG, Reilingen (Germany); Huppmann, G. (eds.) [MTU CFC Solutions GmbH, Muenchen (Germany)

    2007-07-01

    This book presents model-based concepts for process analysis and control on a generalized basis. It is structured as follows: Part I - DESIGN AND OPERATION: MTU's Carbonate Fuel Cell HotModule; Operational Experiences. Part II - MODEL-BASED PROCESS ANALYSIS: MCFC Reference Model; Index Analysis of Models; Parameter Identification; Steady State Process Analysis; Hot spot formation and steady state multiplicities; Conceptual design an Reforming concepts. Part III - OPTIMIZATION AND ADVANCED CONTROL: Model reduction and State estimation; Optimal Control Strategies; Optimization of Reforming Catalyst Distribution.

  12. Quantitative single cell analysis of cell population dynamics during submandibular salivary gland development and differentiation

    Directory of Open Access Journals (Sweden)

    Deirdre A. Nelson

    2013-04-01

    Epithelial organ morphogenesis involves reciprocal interactions between epithelial and mesenchymal cell types to balance progenitor cell retention and expansion with cell differentiation for evolution of tissue architecture. Underlying submandibular salivary gland branching morphogenesis is the regulated proliferation and differentiation of perhaps several progenitor cell populations, which have not been characterized throughout development, and yet are critical for understanding organ development, regeneration, and disease. Here we applied a serial multiplexed fluorescent immunohistochemistry technology to map the progressive refinement of the epithelial and mesenchymal cell populations throughout development from embryonic day 14 through postnatal day 20. Using computational single cell analysis methods, we simultaneously mapped the evolving temporal and spatial location of epithelial cells expressing subsets of differentiation and progenitor markers throughout salivary gland development. We mapped epithelial cell differentiation markers, including aquaporin 5, PSP, SABPA, and mucin 10 (acinar cells; cytokeratin 7 (ductal cells; and smooth muscle α-actin (myoepithelial cells and epithelial progenitor cell markers, cytokeratin 5 and c-kit. We used pairwise correlation and visual mapping of the cells in multiplexed images to quantify the number of single- and double-positive cells expressing these differentiation and progenitor markers at each developmental stage. We identified smooth muscle α-actin as a putative early myoepithelial progenitor marker that is expressed in cytokeratin 5-negative cells. Additionally, our results reveal dynamic expansion and redistributions of c-kit- and K5-positive progenitor cell populations throughout development and in postnatal glands. The data suggest that there are temporally and spatially discreet progenitor populations that contribute to salivary gland development and homeostasis.

  13. Analysis of high efficiency back point contact silicon solar cells

    Science.gov (United States)

    Luque, Antonio

    1988-01-01

    A model has been developed for the analysis of Back Point-Contact (BPC) cells under variable injection level. The analysis has been applied to an experimental cell from Stanford University to allow the extraction of the recombination parameters of this cell. While the bulk SRH recombination and the recombination in the surface and in the emitters are those expected, the Auger constant takes a higher value (2.1 × 10 -30 cm 6/s), than the one usually accepted, and in agreement with the measurements by the Stanford group, for the carrier density involved here. The analysis indicates that best efficiency results are obtained with cells with finely designed emitter dots and well passivated surfaces, made on high resistivity substrates, leading to an upper limit of efficiency obtained at 20 W/cm 2 of about 30.4%. If our technology prevents us from a fine dot delineation (below 5-10 μm) then the highest efficiency is to be expected from the more conventional Interdigitated Back Contact cells with a limit (with our fitted Auger constant) of about 30%. Finally, if the commonly accepted value of the Auger constant (3.8 × 10 -31 cm 6/s) is used this limit is obtained at 50 W/cm 2 and is of 33.1% with a strongly idealized cell. All the efficiencies are at 25°C.

  14. METHOD FOR AUTOMATIC ANALYSIS OF WHEAT STRAW PULP CELL TYPES

    Directory of Open Access Journals (Sweden)

    Mikko Karjalainen,

    2012-01-01

    Full Text Available Agricultural residues are receiving increasing interest when studying renewable raw materials for industrial use. Residues, generally referred to as nonwood materials, are usually complex materials. Wheat straw is one of the most abundant agricultural residues around the world and is therefore available for extensive industrial use. However, more information of its cell types is needed to utilize wheat straw efficiently in pulp and papermaking. The pulp cell types and particle dimensions of wheat straw were studied, using an optical microscope and an automatic optical fibre analyzer. The role of various cell types in wheat straw pulp and papermaking is discussed. Wheat straw pulp components were categorized according to particle morphology and categorization with an automatic optical analyzer was used to determine wheat straw pulp cell types. The results from automatic optical analysis were compared to those with microscopic analysis and a good correlation was found. Automatic optical analysis was found to be a promising tool for the in-depth analysis of wheat straw pulp cell types.

  15. Cell wall staining with Trypan blue enables quantitative analysis of morphological changes in yeast cells

    DEFF Research Database (Denmark)

    Liesche, Johannes; Marek, Magdalena; Günther-Pomorski, Thomas

    2015-01-01

    with Trypan Blue, which emits strong red fluorescence upon binding to chitin and yeast glucan; thereby, it facilitates cell wall analysis by confocal and super-resolution microscopy. The staining pattern of Trypan Blue was similar to that of the widely used UV-excitable, blue fluorescent cell wall stain...... Calcofluor White. Trypan Blue staining facilitated quantification of cell size and cell wall volume when utilizing the optical sectioning capacity of a confocal microscope. This enabled the quantification of morphological changes during growth under anaerobic conditions and in the presence of chemicals...

  16. A Quantitative Analysis of Photovoltaic Modules Using Halved Cells

    Directory of Open Access Journals (Sweden)

    S. Guo

    2013-01-01

    Full Text Available In a silicon wafer-based photovoltaic (PV module, significant power is lost due to current transport through the ribbons interconnecting neighbour cells. Using halved cells in PV modules is an effective method to reduce the resistive power loss which has already been applied by some major PV manufacturers (Mitsubishi, BP Solar in their commercial available PV modules. As a consequence, quantitative analysis of PV modules using halved cells is needed. In this paper we investigate theoretically and experimentally the difference between modules made with halved and full-size solar cells. Theoretically, we find an improvement in fill factor of 1.8% absolute and output power of 90 mW for the halved cell minimodule. Experimentally, we find an improvement in fill factor of 1.3% absolute and output power of 60 mW for the halved cell module. Also, we investigate theoretically how this effect confers to the case of large-size modules. It is found that the performance increment of halved cell PV modules is even higher for high-efficiency solar cells. After that, the resistive loss of large-size modules with different interconnection schemes is analysed. Finally, factors influencing the performance and cost of industrial halved cell PV modules are discussed.

  17. A Bibliometric Analysis of Publications on Pluripotent Stem Cell Research

    Directory of Open Access Journals (Sweden)

    Changshuan L. Lin

    2015-04-01

    Full Text Available Objective: Human pluripotent stem cells are self-renewing cells with the ability to differentiate into a variety of cells and are viewed to have great potential in the field of regenerative medicine. Research in pluripotent stem cells holds great promise for patient specific therapy in various diseases. In this study, pluripotent stem cell articles published from 1991 to 2012 were screened and retrieved from Science Citation Index Expanded (SCI-EXPANDED. Materials and Methods: In this retrospective study, the publication trend, citation trends for top articles, distributions of journals and Web of Science categories were analyzed. Five bibliometric indicators including total articles, independent articles, collaborative articles, first author articles, and corresponding author articles were applied to compare publications between countries and institutions. Results: The impact of top articles changed from year to year. Top cited articles in previous publication years were not the same as recent years. "Induced pluripotent stem cell (s" and "embryonic stem cell (s" were the most used author keywords in pluripotent stem cell research. In addition, the winner of the Nobel Prize in physiology or medicine in 2012, Prof. Shinya Yamanaka, published four of the top ten most frequently cited articles. Conclusion: The comprehensive analysis of highly cited articles in the stem cell field could identify milestones and important contributors, giving a historic perspective on scientific progress.

  18. Segmentation and Analysis of Cancer Cells in Blood Samples

    Directory of Open Access Journals (Sweden)

    Arjun Nelikanti

    2015-10-01

    Full Text Available Blood cancer is an umbrella term for cancers that affect the blood, bone marrow and lymphatic system. Acute Lymphoblastic Leukemia (ALL is one of the kinds of blood cancer which can be affected at any age in the humans. The analysis of peripheral blood samples is an important test in the procedures for the diagnosis of leukemia. In this paper the blood sample images are used and implementing a clustering algorithm for detection of the cancer cells. This paper also implements morphological operations and feature extraction techniques using MATLAB for the analysis of cancer cells in the images.

  19. Stress analysis for wall structure in mobile hot cell design

    Energy Technology Data Exchange (ETDEWEB)

    Bahrin, Muhammad Hannan, E-mail: hannan@nuclearmalaysia.gov.my; Rahman, Anwar Abdul, E-mail: anwar@nuclearmalaysia.gov.my; Hamzah, Mohd Arif, E-mail: arif@nuclearmalaysia.gov.my; Mamat, Mohd Rizal; Azman, Azraf; Hasan, Hasni [Prototype and Plant Development Centre, Technical Services Division, Malaysian Nuclear Agency (Malaysia)

    2016-01-22

    Malaysian Nuclear Agency is developing a Mobile Hot Cell (MHC) in order to handle and manage Spent High Activity Radioactive Sources (SHARS) such as teletherapy heads and irradiators. At present, there are only two units of MHC in the world, in South Africa and China. Malaysian Mobile Hot cell is developed by Malaysian Nuclear Agency with the assistance of IAEA expert, based on the design of South Africa and China, but with improved features. Stress analysis has been performed on the design in order to fulfil the safety requirement in operation of MHC. This paper discusses the loading analysis effect from the sand to the MHC wall structure.

  20. 2D-immunoblotting analysis of Sporothrix schenckii cell wall

    Directory of Open Access Journals (Sweden)

    Estela Ruiz-Baca

    2011-03-01

    Full Text Available We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70 was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glycoprotein was present only in yeast-like cells. In addition to the Gp70, the wall from filament cells showed four proteins with molecular weights of 48, 55, 66 and 67 kDa, some of which exhibited several isoforms. To our knowledge, this is the first 2D-immunoblotting analysis of the S. schenckii cell wall.